LOCUS       DACCDF010000001        78969 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCDF010000001 DACCDF010000000
VERSION     DACCDF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.001"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            14..1141
                     /locus_tag="IVK51_000001"
     CDS             14..1141
                     /locus_tag="IVK51_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6429630.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1235..3070)
                     /gene="lepA"
                     /locus_tag="IVK51_000002"
     CDS             complement(1235..3070)
                     /gene="lepA"
                     /locus_tag="IVK51_000002"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP6429631.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            3217..4437
                     /locus_tag="IVK51_000003"
     CDS             3217..4437
                     /locus_tag="IVK51_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429632.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            4457..4951
                     /locus_tag="IVK51_000004"
     CDS             4457..4951
                     /locus_tag="IVK51_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6429633.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            4944..5615
                     /locus_tag="IVK51_000005"
     CDS             4944..5615
                     /locus_tag="IVK51_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429634.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(5766..8375)
                     /gene="clpB"
                     /locus_tag="IVK51_000006"
     CDS             complement(5766..8375)
                     /gene="clpB"
                     /locus_tag="IVK51_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP6429635.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8626..9672)
                     /locus_tag="IVK51_000007"
     CDS             complement(8626..9672)
                     /locus_tag="IVK51_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP6429636.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(9683..9955)
                     /locus_tag="IVK51_000008"
     CDS             complement(9683..9955)
                     /locus_tag="IVK51_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP6429637.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(9990..10898)
                     /locus_tag="IVK51_000009"
     CDS             complement(9990..10898)
                     /locus_tag="IVK51_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP6429638.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(11030..12262)
                     /gene="pepT"
                     /locus_tag="IVK51_000010"
     CDS             complement(11030..12262)
                     /gene="pepT"
                     /locus_tag="IVK51_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP6429639.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(12277..13398)
                     /locus_tag="IVK51_000011"
     CDS             complement(12277..13398)
                     /locus_tag="IVK51_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP6429640.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(13395..14096)
                     /locus_tag="IVK51_000012"
     CDS             complement(13395..14096)
                     /locus_tag="IVK51_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP6429641.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(14240..14680)
                     /locus_tag="IVK51_000013"
     CDS             complement(14240..14680)
                     /locus_tag="IVK51_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP6429642.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(14809..15735)
                     /gene="dnaI"
                     /locus_tag="IVK51_000014"
     CDS             complement(14809..15735)
                     /gene="dnaI"
                     /locus_tag="IVK51_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP6429643.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(15735..17108)
                     /locus_tag="IVK51_000015"
     CDS             complement(15735..17108)
                     /locus_tag="IVK51_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP6429644.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(17131..17622)
                     /gene="nrdR"
                     /locus_tag="IVK51_000016"
     CDS             complement(17131..17622)
                     /gene="nrdR"
                     /locus_tag="IVK51_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP6429645.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(17934..18266)
                     /locus_tag="IVK51_000017"
     CDS             complement(17934..18266)
                     /locus_tag="IVK51_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6429646.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(18318..18947)
                     /locus_tag="IVK51_000018"
     CDS             complement(18318..18947)
                     /locus_tag="IVK51_000018"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP6429647.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            complement(18911..19747)
                     /gene="mutM"
                     /locus_tag="IVK51_000019"
     CDS             complement(18911..19747)
                     /gene="mutM"
                     /locus_tag="IVK51_000019"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP6429648.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(19792..22437)
                     /gene="polA"
                     /locus_tag="IVK51_000020"
     CDS             complement(19792..22437)
                     /gene="polA"
                     /locus_tag="IVK51_000020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP6429649.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(22563..23246)
                     /locus_tag="IVK51_000021"
     CDS             complement(22563..23246)
                     /locus_tag="IVK51_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP6429650.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(23239..23724)
                     /locus_tag="IVK51_000022"
     CDS             complement(23239..23724)
                     /locus_tag="IVK51_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP6429651.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(24031..25365)
                     /locus_tag="IVK51_000023"
     CDS             complement(24031..25365)
                     /locus_tag="IVK51_000023"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP6429652.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(25496..26257)
                     /locus_tag="IVK51_000024"
     CDS             complement(25496..26257)
                     /locus_tag="IVK51_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6429653.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(26273..26755)
                     /locus_tag="IVK51_000025"
     CDS             complement(26273..26755)
                     /locus_tag="IVK51_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP6429654.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(26775..28955)
                     /locus_tag="IVK51_000026"
     CDS             complement(26775..28955)
                     /locus_tag="IVK51_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP6429655.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(29099..29830)
                     /locus_tag="IVK51_000027"
     CDS             complement(29099..29830)
                     /locus_tag="IVK51_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP6429656.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(29899..30840)
                     /locus_tag="IVK51_000028"
     CDS             complement(29899..30840)
                     /locus_tag="IVK51_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP6429657.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(31020..31388)
                     /locus_tag="IVK51_000029"
     CDS             complement(31020..31388)
                     /locus_tag="IVK51_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6429658.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            complement(31999..33390)
                     /locus_tag="IVK51_000030"
     CDS             complement(31999..33390)
                     /locus_tag="IVK51_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP6429659.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(33603..33989)
                     /locus_tag="IVK51_000031"
     CDS             complement(33603..33989)
                     /locus_tag="IVK51_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP6429660.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(34045..34668)
                     /locus_tag="IVK51_000032"
     CDS             complement(34045..34668)
                     /locus_tag="IVK51_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP6429661.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(34732..35400)
                     /locus_tag="IVK51_000033"
     CDS             complement(34732..35400)
                     /locus_tag="IVK51_000033"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6429662.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(35412..35852)
                     /locus_tag="IVK51_000034"
     CDS             complement(35412..35852)
                     /locus_tag="IVK51_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429663.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(35911..36582)
                     /locus_tag="IVK51_000035"
     CDS             complement(35911..36582)
                     /locus_tag="IVK51_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6429664.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(36767..37165)
                     /locus_tag="IVK51_000036"
     CDS             complement(36767..37165)
                     /locus_tag="IVK51_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP6429665.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(37162..37935)
                     /locus_tag="IVK51_000037"
     CDS             complement(37162..37935)
                     /locus_tag="IVK51_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6429666.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(37932..38615)
                     /locus_tag="IVK51_000038"
     CDS             complement(37932..38615)
                     /locus_tag="IVK51_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP6429667.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(38782..39669)
                     /locus_tag="IVK51_000039"
     CDS             complement(38782..39669)
                     /locus_tag="IVK51_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6429668.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(39666..40535)
                     /locus_tag="IVK51_000040"
     CDS             complement(39666..40535)
                     /locus_tag="IVK51_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6429669.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(40672..41958)
                     /locus_tag="IVK51_000041"
     CDS             complement(40672..41958)
                     /locus_tag="IVK51_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6429670.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            42231..42809
                     /locus_tag="IVK51_000042"
     CDS             42231..42809
                     /locus_tag="IVK51_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6429671.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            42809..43738
                     /locus_tag="IVK51_000043"
     CDS             42809..43738
                     /locus_tag="IVK51_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6429672.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            43735..45471
                     /locus_tag="IVK51_000044"
     CDS             43735..45471
                     /locus_tag="IVK51_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6429673.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(45534..46370)
                     /gene="pheA"
                     /locus_tag="IVK51_000045"
     CDS             complement(45534..46370)
                     /gene="pheA"
                     /locus_tag="IVK51_000045"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP6429674.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(46367..46876)
                     /locus_tag="IVK51_000046"
     CDS             complement(46367..46876)
                     /locus_tag="IVK51_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP6429675.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(46869..48164)
                     /gene="aroA"
                     /locus_tag="IVK51_000047"
     CDS             complement(46869..48164)
                     /gene="aroA"
                     /locus_tag="IVK51_000047"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6429676.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(48177..49268)
                     /locus_tag="IVK51_000048"
     CDS             complement(48177..49268)
                     /locus_tag="IVK51_000048"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP6429677.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(49285..50451)
                     /gene="aroC"
                     /locus_tag="IVK51_000049"
     CDS             complement(49285..50451)
                     /gene="aroC"
                     /locus_tag="IVK51_000049"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP6429678.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(50453..51517)
                     /gene="aroB"
                     /locus_tag="IVK51_000050"
     CDS             complement(50453..51517)
                     /gene="aroB"
                     /locus_tag="IVK51_000050"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP6429679.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(51550..52572)
                     /gene="aroF"
                     /locus_tag="IVK51_000051"
     CDS             complement(51550..52572)
                     /gene="aroF"
                     /locus_tag="IVK51_000051"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP6429680.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(53231..54205)
                     /locus_tag="IVK51_000052"
     CDS             complement(53231..54205)
                     /locus_tag="IVK51_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP6429681.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(54186..56069)
                     /locus_tag="IVK51_000053"
     CDS             complement(54186..56069)
                     /locus_tag="IVK51_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP6429682.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(56087..58312)
                     /locus_tag="IVK51_000054"
     CDS             complement(56087..58312)
                     /locus_tag="IVK51_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP6429683.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            58427..59461
                     /locus_tag="IVK51_000055"
     CDS             58427..59461
                     /locus_tag="IVK51_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6429684.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(59528..60541)
                     /locus_tag="IVK51_000056"
     CDS             complement(59528..60541)
                     /locus_tag="IVK51_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6429685.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(60619..60807)
                     /locus_tag="IVK51_000057"
     CDS             complement(60619..60807)
                     /locus_tag="IVK51_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP6429686.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(61247..61375)
                     /locus_tag="IVK51_000058"
     CDS             complement(61247..61375)
                     /locus_tag="IVK51_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6429687.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(61378..62751)
                     /gene="rlmD"
                     /locus_tag="IVK51_000059"
     CDS             complement(61378..62751)
                     /gene="rlmD"
                     /locus_tag="IVK51_000059"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6429688.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(62862..63917)
                     /locus_tag="IVK51_000060"
     CDS             complement(62862..63917)
                     /locus_tag="IVK51_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6429689.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(63927..65357)
                     /gene="gatB"
                     /locus_tag="IVK51_000061"
     CDS             complement(63927..65357)
                     /gene="gatB"
                     /locus_tag="IVK51_000061"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP6429690.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(65354..66826)
                     /gene="gatA"
                     /locus_tag="IVK51_000062"
     CDS             complement(65354..66826)
                     /gene="gatA"
                     /locus_tag="IVK51_000062"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP6429691.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(66826..67131)
                     /gene="gatC"
                     /locus_tag="IVK51_000063"
     CDS             complement(66826..67131)
                     /gene="gatC"
                     /locus_tag="IVK51_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP6429692.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(67237..69273)
                     /gene="ligA"
                     /locus_tag="IVK51_000064"
     CDS             complement(67237..69273)
                     /gene="ligA"
                     /locus_tag="IVK51_000064"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP6429693.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(69290..71524)
                     /gene="pcrA"
                     /locus_tag="IVK51_000065"
     CDS             complement(69290..71524)
                     /gene="pcrA"
                     /locus_tag="IVK51_000065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP6429694.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            71679..73238
                     /locus_tag="IVK51_000066"
     CDS             71679..73238
                     /locus_tag="IVK51_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP6429695.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(73349..75280)
                     /locus_tag="IVK51_000067"
     CDS             complement(73349..75280)
                     /locus_tag="IVK51_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6429696.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(75298..76212)
                     /gene="pfkB"
                     /locus_tag="IVK51_000068"
     CDS             complement(75298..76212)
                     /gene="pfkB"
                     /locus_tag="IVK51_000068"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6429697.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(76209..76961)
                     /locus_tag="IVK51_000069"
     CDS             complement(76209..76961)
                     /locus_tag="IVK51_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6429698.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(77121..77330)
                     /locus_tag="IVK51_000070"
     CDS             complement(77121..77330)
                     /locus_tag="IVK51_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429699.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(77341..78669)
                     /locus_tag="IVK51_000071"
     CDS             complement(77341..78669)
                     /locus_tag="IVK51_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429700.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT        21889 a        16996 c        14091 g        25993 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcagaac ggccatgaag gcttcttgcg gaacttcgac ggagccgatt tgtttcatcc
     1321 gcttcttccc ttctttttgt ttctctaaga gtttacgttt acgagaaaca tctccaccat
     1381 aacatttagc caagacgttt ttacgcaagg ctttgatgtc tgaacgggca acgatttttt
     1441 gtccaatagc tgcttggatt ggcacctcaa attgttggcg tgggattagt tttttcagtt
     1501 tttcaacgat tgcttttcca cgttcatagg caaagtctcg gtgaacgata aagcttaatg
     1561 catcgacttt ttctccattc agcaagatgt ccatcttcac taacttgctt ttttgataac
     1621 cagacatttc ataatccaat gaggcatagc ctttcgtgct tgatttcaat ttgtcgaaaa
     1681 agtcaaagac aatttcagaa agcggaatat tatagacgac atttactcgg taatcatcta
     1741 aataatccat cgtaatgaat tctccccgtt ttcgttgaga aagttccatt actgcaccga
     1801 caaaatcatt tgggaccatg atctgtgctt tgacgaatgg ttcttctaca tcctgaatcg
     1861 ttacaggttc aggaaaatct gccgggttat ctactgtagc agtcgtacca tccgttttat
     1921 taacatggta aataacggat ggtgcagtcg tgatcaattc tagattgaat tcccgttcca
     1981 agcgttcttg gacgacatcc atatgaagaa gtccgaggaa tccacagcgg aacccaaaac
     2041 ctaatgcttg agaggtttct ggttcgaatt gtaaagcagc atcattcaat tgcagttttt
     2101 ctaaagcttc ccgcaaatcg ttgtaacgag aagtatcaat tggataaaga ccacaataaa
     2161 ccatcggatt catttttcgg taacctggta aagcttcagc ggcaggattg tctgctaacg
     2221 ttactgtatc ccctactcgt gtatcttgga cagttttgat acttgctgtg atataaccga
     2281 catcaccgac catcaaaaag tctctagcaa ctgcttttgg cgagaagacc ccgacctctg
     2341 taacatcaaa tgttttgcca ttactcatca gtttgatttt gtctccgggt ttgaccatac
     2401 cgtccgtgat ccggacattt agaacaacac cgcggtaact atcataaata gagtcaaaga
     2461 tcaaagcttt tagcggtgca tctaagtctc cacttggtgc aggaacatat tccacgatct
     2521 gctcgagaat atcttcaatc ccaatccctg atttggcact ggctaataca gcttcactag
     2581 catcgattcc gattacgtct tcgatttctt gacggacacg ttcgggatct gctgcgggta
     2641 ggtcgatttt attgatgact ggcaggatct ccaaatcatt gtctaacgct aaataaacat
     2701 tggccaatgt ttgtgcttcg attccttgtg ctgcatcgac cactaaaacg gcaccctcac
     2761 aggcagcaag gcttcgagag acttcatagg tgaagtcgac gtgccctggg gtgtcaatca
     2821 agtgaaagat atatgtttct ccatttttcg ctgtgtaatt gagttctaca gcatttaatt
     2881 taatcgtgat tccacgttca cgttccaaat ccatcgagtc taacagctga tcttgcattt
     2941 cgcgagacgt cacagtgtgc gtcatttcta aaatacgatc ggccaatgta gatttcccat
     3001 ggtcgatatg agcgatgatc gaaaaattac gaatctgctc ttggcgttct ttcattttct
     3061 ttatatccat agcgtctcct cttttcatat aatcagcact ttccattata ccaacgaaaa
     3121 gcagtaattt aaagagtttt taattagtca gctatagaag ttttttttgt agaattttgc
     3181 tatactgtta tcaagctaat aaatggagat gaaaaaatgg atcaaaaaga ttttagagaa
     3241 aatcttgaat taaaaccggt cactcaagaa catatcgatc aattcaatga attgctttcc
     3301 tatgtgttcc aagtgactga agcagacatc gaggaaagcg ggtttgagaa taaacgagct
     3361 tttatccgtt ccaaaaaacc gattttggaa gtatcgaaag tgttcggatg gttccacgaa
     3421 aataaactga tctctcaaat cgctatctat ccttgtgaag tcaatattca cggtgcacgt
     3481 tataaaatgg gtggtgtgac tggagttgga acttatccag aatatgctaa ccatggattg
     3541 atgcaagatc taattatcgt tgcattggat aatatgcgca agaataagca atggatctct
     3601 tatttgtatc cttacagtat cccctactat cggcgtaaag gatgggaaat catgtctgat
     3661 aaactttcct ttaaaatcag agatacacag ctacctaaac aaatcccagt aagtggtatg
     3721 gttgagcgat taccagttga tcatccagac gtattttccg tttatgacaa atttgcccaa
     3781 caaaaccatg gtgctttatt ccgcagtgat tttcattggg aagagtattg gcgtttcgaa
     3841 aatgaagatg agcgcacagc cgctgtctac tacgatagca accatgaacc aagaggtgtt
     3901 ctgttctact gggtagctga agaaatattt cacgtaaaag aaatgttcta tcttgatcaa
     3961 gaagctcgaa atggcttatg gaactttatt tccgcacatt tttccatgat ctactgggta
     4021 catggagata tttataaaaa tgagccttta gcatttttga ttgaagatag tcagatcaaa
     4081 gagcagatcg aaccatattt tatggctcgg atcgttgatg taaaagaatt ccttcaacgg
     4141 tttccgtttg ttggaacagc tgatgctttt catttcatca ttgaagatcc tgtcgctcct
     4201 tggaataatg gtgtcttcgc tttaacatgg gatgaacagg gacaagtccg tgtcttaaat
     4261 gaacctatag gaaaatccgt tcgactggat atccaaacat tgacttgctt gatgatgaat
     4321 tatcgccgtg cttcttattt ggcaagaatc gaacggttag aaacagatga agaaacactg
     4381 aagtcactag agcgtatcat tccaaacatg gaagcttact ttagcgatta tttttgataa
     4441 aaaggatgat gtttagatga aaatttattt tgccgcacca atgtttgcta aaagtgattt
     4501 gttatataac caacagttag ttaaagaaat cagagagctc tcttctgagc tttcgatcta
     4561 tcttcctcaa gaaaatgaag caatcaatga caaaactgct tatgcagaca gtcgaatgat
     4621 tgctcttgct gacacagaaa aagtgttggc tagtgattta atgattgctt tactagatgg
     4681 gctgacgatc gatgccgggg tagcatcgga aatcggtgtg gcttatgcga aaggcatccc
     4741 agtgatcggc ttatatactg atactcgaca gcaaggcgga acccacccga aaaaaatcgc
     4801 tgcactacaa gaaacagctg aaaatcaatt tcactacctg aacttatata caataggttt
     4861 gatcaaattg aacggaaaag tggtttcttc agaaattgag ttgctttcag aagtaaaaag
     4921 atttttagat ggagggactt tcagtgatta aagaaatcaa aaaggtgcaa atagctttac
     4981 ttgcttttgg tgtattcgtt gtctgctata atttttatga atttatcaca caaaaatatt
     5041 cgacttctca aggaatcaca tttatcgtgg aatctttatt agggattgca ttgattttca
     5101 tgccacaagt cattttgaaa gttttcaaac ttaaaatacc agcagcaatc gttttgtttt
     5161 actggttttt cttgttcatc tctgtctttt taggcacagg gatgcatttg atcagtatca
     5221 tctctttttg ggacaaaatc ttacatgcgg tgagtccaat ggtcctgaca gctcttggct
     5281 atggattgat tggttacttg atgaaagatg ctgagatatc aaaaaccagt ccttggttgt
     5341 tcctcttatt cggctttgcg tttgctggac tttgcggcgt gttttgggag ttctgggaat
     5401 tcttatgcga ccagttctta ggcatgaatc ttcaacgttt tgctgcttct gatggcacac
     5461 tgtttgtcgg acgagcagca ttgatggata cgatgggcga cttgctgaca aatacgatag
     5521 gtgctgcctt aatgggattg tttgcatggt ctcaaagtaa aaaagatgag cgttactttg
     5581 aaagttataa attagaaaaa gtgaaaaata cctaataaaa gaggttgtga cagaagcggg
     5641 cagcttcaag aaatttcggc ttatttttcg aagaaactac ttctggaaca ctgtttattt
     5701 gaaaaaagga ggtctgacac tagttgtgtc agaccccttt tttcttttat agtctcaatg
     5761 gaagattaga cgatttcttc tagctcttct gttttgaaat gaagttgtcc atcaagtaaa
     5821 gtaatcgtta cttttgattt aggcataaca tgtcctgcaa cgatttcttt agctagtggt
     5881 gtttctactt ccttagtgat aaatcgtttc aatggtcttg caccatatgc tggttcataa
     5941 gcattttcag caatccacgt tttggcttca tcactgattg tcagcaggat ttcttgatgt
     6001 tccagacgtt gtgccaattg cgccaccatt ttgtcaacga ttcctttcac gttgtctagg
     6061 ctcaatggtg taaataaaat cgtgtcgtcg attcggttca agaattctgg tttgaagttt
     6121 ccacgtaata gcgtgttgac ttgttcagcc actgcttctg gaatcgttcc atcagcagtc
     6181 acgccttcca gcagtaactg tgaaccgata ttgctggtca tgatcagaac cgtatttttg
     6241 aaatctacta ctcgcccttt tgaatcagtc aagcgtccgt catctaggac ttgtaacaag
     6301 atattgaaga catcgggatg cgctttttcg atttcatcca acaagacgat tgtataagga
     6361 tttcgtctga cagcttccgt taattgacca ccttcttcgt aacctacata gcctggagga
     6421 gctccgacta gacgagacac agcatgtttt tccatatatt cactcatatc gatccgtacc
     6481 atatgatctt ctgagtcaaa caagttctca gctaacgctt tcgctaattc ggtttttccg
     6541 acaccagttg gtcctaggaa aaggaaagaa cctagaggac gatttgggtc ttgcaatcca
     6601 gcccgtgaac gaattaccgc gtcactgacc gcatcaactg cttcatcttg accaatcact
     6661 cgtttatgaa gtgtttcatt caatttaatt agtttttctc gttcaccttc tacaagtttc
     6721 gttacgggga tacccgttaa tctgccaacg acttgagcaa tctcattttc ggtaacggac
     6781 tcttgaacca tcttaatctc gctgtctttt gctttagctt ctaattcctt caattctttt
     6841 tctaattgag gtatcgtacc atgacgcaat acagcagcgc gttctaaatc atagttattt
     6901 tctgcatctt ctaattcgtg ttttgcttta tcgatttcag cacgttttgc tgaaacagaa
     6961 ttgacttctt ctttttctgt ttcccactgc atcttcatcg catttgcttc ttcacgaagt
     7021 tcagctaatt cttcttgcaa atttttcaag cgttttttac ttgcatcatc tgattctttt
     7081 ttcagtgcag cttcttcgat ttccaactgc atcaaacgtc tagtaacttg atctaattcc
     7141 gttggcatag agttcatttc tacccgaatc gttgcactgg cttcatcgat caaatcgatt
     7201 gctttgtccg gtaagaaccg atcagtaata taacggtcag ataaagtagc tgctgctact
     7261 aatgcgttgt catggatgtt aacaccatgg tggatctcaa aacgttcttt taatccacgc
     7321 agaatactga tcgtatcttc cactgttggt tctttgacta ataccttttg aaagcgacgt
     7381 tccaacgctt tgtctttttc catatattga cggtactcat ctaaagttgt ggcaccgatc
     7441 aaatgtaatt caccgcgagc aagcattggt ttcaacagat ttccggcatc catacttcct
     7501 tctgtttttc ctgcccctac gatattgtgg atttcatcga tgaacaagag gatacgtcca
     7561 tcactttttt tgacttcttt caagactgct tttagtcttt cttcaaattc accacggaat
     7621 tttgctccag cgatcaatgc acccatatct aatgaaaaaa tcgttttatc ttttaaattt
     7681 tcaggcacat ctttgcgtac gatacgttga gcaagccctt caacgattgc cgttttccct
     7741 acaccgggtt ctcctatcag aacaggatta ttttttgttt ttcgtgaaag aatacggatg
     7801 acatcacgga tttcttcatc acgaccgatg attggatcca tttttccgct tttgacttgc
     7861 tgtactaaat caacgccgta tttttctaat gctttgtatt gttcttcttg gttttgtgag
     7921 gtcactcttt ctcctcctct catctcttcg atgtttttac gcaattcttt ttcagataat
     7981 ccgtttttat tcaaataaac agttaacgga taatttttta atttcatcaa cgctaaaata
     8041 acgacttccg ttgaaagaaa ctcgtcgccg aagctttcac gtatttggtc tgcttcattc
     8101 aataatcgaa agaaattctg gctgaggttt tgtccatatt ggacgtttcc accagaaaca
     8161 actggatact catccagtag acgatcaatt tcatgttcaa aagattcgac atccaatccg
     8221 gcatccgtat agaaatttcg accaaaatga ttaggttgta aaaagatttt ccatacatgg
     8281 gcaatgtcga tatcttgatg ttttcgagtg accgcaatct gttgagcttc agcaatcgct
     8341 tcttgaagcg tcgtagtcat tttctcaata ttcatacagg tataccctcc taattaaagg
     8401 taagatcact tatttctttg atctttacaa aaactattat atcgctttgg tcaattttgg
     8461 tcaaaatata cacatggttt tttgactttt tttctcgact tatataaatc ttaaaaaaac
     8521 aaaattgatt gtatgtatat aaaaaaagag ccaaacaaac gtaaaattat cttatccata
     8581 acgtttttct ttgatttgat aaaaagggta gatcttataa aagacctacc cttgattttc
     8641 tttattctct ttttttactt ctattatttc ttctttgatc actcttcgcc gttcagcaat
     8701 cacatacaca aatccataga gaaatggcaa gataaaggaa ctgaaacgat acagtaaaag
     8761 cgaagaaact gcgtcaactg ttccaacaac tggtctgaat aataacaaat agacaaattc
     8821 aaaggaacca ataccagcag gcgtaggaat aacccctgaa agcactactg caaagctgat
     8881 aaacgaaatg gtcagtagaa aatctatttt aggatggttt tcaatcagaa tcaaataagg
     8941 aagtacatac caaaatacaa gttttaacat attccataga taaatccgca atagcgcagt
     9001 cctgtcttgg ataatggttt ggaccgtttc ccttaaagag tagatctgct gattgcatgt
     9061 atccacccat ttccgcaaac gctcactttt aaataactta ttcgataagc tgacaaaaaa
     9121 tatttgaaca ttcaagctga cggacatcgc caataaaaag gcaacaatca cgatcgtcaa
     9181 aaggatacca gcgatgataa accagatcat tgatggacca ttagaataaa aaagagagaa
     9241 ttggatgacc aagccagcta atgcataagt gatcactgcc agtttgtaca taatcatatg
     9301 gagtgctgtc acccctgctc cctgggatag cttcattcct tttttgttat aaaaataaat
     9361 ctccgagatc agcgttcctg taccaaacga aacgattcgg taaaacgcaa tatagctaga
     9421 ggtcaagaac ccatctttcg ttgtaaaatt cggcgcgaaa ggttcagcaa tttctttgat
     9481 tgagcgccct tcagcgattt ggtaaactgt ccctaatata acagccgctg ccaaaacaac
     9541 aacactggta gacagcaatt cggcaaagat atctgcaaat gattgattga ttaaataata
     9601 aataatggca aaaatcacaa tcagcaaaag cagattcaga atgattttag ttttcgttcc
     9661 tttagacgac atctcctgct ccttaatgga aaaaataaat gagaatcaaa tcaacaagta
     9721 cacctgcagc gaatcctata gtaaacttct ttttccgagt tttatgatgg aaaacttttc
     9781 gagagatcaa tcctcccaaa ccaccgccta acaggctgac aaatatcaaa ttattctcag
     9841 gaacacgcca gccgctagtt ctagcctgat gtttatcata ccccatcaga caaaataagt
     9901 aaaaattcac aatgaaaagg taaaaccaca aaagattgtg taacaataca tccattactc
     9961 ttcttccaac tttctgttaa ctactatttc taagcttttt cttctttctt tggtgaataa
    10021 actgttaatc cttttgatac aacagataat tcaactggaa gatcatctgt cgtgtctcca
    10081 tccgttcgag tgcccacaga tttttcctgc gcttggattt tcatgcgttt ggctgtgaaa
    10141 taagtaatgc cggggatcgt atagaatttg cctctcatga ttctaggcaa ataaaaaata
    10201 aatcggaaaa tatctaagga aggtacgatc gttacatgaa attttccatc atctagggtc
    10261 gctgagtcgt caaaatttgt aaaaccgccc acggagttag acatcgtgac tgtcaacagt
    10321 tgggattttc ctttccaatg ctcctcgtct gtttctaaat ccaaatgata ttttttcttc
    10381 ttcagcaata acttaaaaaa cttcttgata aagatccacg agccgtaacg ctgtttttct
    10441 tcttgactga tccaaactgc tgtatctgct aagattccga ttgtcagtgt agagataatg
    10501 acttcatcat tgacttgacc atagtctatt ttccgtacat tgccttccag gatcacgtca
    10561 gcagcttctt cttttttgag gggaatctca agaacacgtg caaggttgtt gacagttcca
    10621 cctggaagta atccgacatg gtattgagat aatgtttttt ggaacgcttg gacgatatga
    10681 tgaatcgtgc cgtctccacc tataattact aaagtatcag cttctatggc ttctgctttc
    10741 ttgactaatt ccgcatcatc aatcgatggg ccagtttcag ctagttctac atataattct
    10801 ggctgctttt cttttacata atcttgaaac cagcgtgcta atttctcacc ctcatctttc
    10861 cctgaacttt tgttataaaa aactagcagc ttttccattc tttacctcct atttactctt
    10921 caaaaaagag ggcgggacaa ccaattccat tgtcctgccc tttagcctta gacagatgac
    10981 attccatcat tgggacgaac gccttgtcat aagctgttct ttttcgattt taactctttg
    11041 tgttcaattc tgcaattttt acaatcacgt ctgttgcttt ttccattact tgtaaagaaa
    11101 caaattcgaa tcgtccgtgc atattttctc ctccggcaaa tagatttggc gtcggaatac
    11161 ccatgtaaga gatttttgaa ccatctgttc ctccacgaac tggttcgatc actggagaga
    11221 taccaagctc gatcattgct tgtttcgcta attccacgat gctcatatct ttttcaatga
    11281 tttctttcat gttgtaatat tgatcataca tatctacttt gattcgttct tgatcaaatc
    11341 gctgattcaa ctcttcttgg atatgtgtga tttgtgcttt gcgtgcttcg aatttttcac
    11401 gatcatgatc gcgaatgata taactcatag aagcttcttc aggattccca tccaatgcca
    11461 tcaaatggaa gaaaccttcg tatccttccg ttttttctgg aacttcatcg gctggaagct
    11521 gattatgaaa atcgattgct aactgtagag cattgatcat tgtatctttt gccgtccctg
    11581 gatgtacatt tttcccttgg atcgtaatat ttgcttgagc agcactaaac gtttcatact
    11641 gcaactcacc taaaggacca ccatcgactg tataagcaaa atctacatcg aattgctcaa
    11701 catcgaattt atccgctccg acaccgattt cttcatcagg accaaaagcc actttgatgg
    11761 taccgtgagg gatagaagga ttattgatca agatctccat tgcagtcatg atttccgcaa
    11821 tccctgattt gtcatctgct cccagtaatg tactgccatc tgttgtgatc aatgtgtggc
    11881 cttcataatt tttcaaattt ggaaaatctt ttgtattcaa tgtatagcgt tcttccgcat
    11941 ctagtacgat cgtagactcg ccatcatagt tttcaatgat ttgtggattg actcctactg
    12001 cattaaaatc tgctgtatcc atgtgggaaa taaatccaat ggaacgcact tcatgatcaa
    12061 cattgcttgg taatgttccc ataacgaatc cgttatcttc attatagaaa acatcagcta
    12121 agcctaactc ttctaattct tttttcaatt cttgcgcaaa agcaacttgt gtctgagtcg
    12181 atggagtcgt tttgcttgtt gcatcggagc gtgtttctgt tttgacatat cgtaaaaaac
    12241 gtggcaaaag attttcgtac atagtattct ctccttttaa ttaaattgaa atgggtttgt
    12301 atttgtttca gaaacgaaaa agttaatatc ccaattttct tcttgtttcc aagattcaaa
    12361 tttctcgata aatcgttgct tgcataacga ttcaatataa tgaccgggat caattgcaat
    12421 caacccagcg ctttgcatat catgagccgt atgataatag atatctcccg tgatatagac
    12481 atctgctctt tgggcgattg cttgcggata aaatttttca ccgcttccac cgcaaatcgc
    12541 aatgcgcttc acagaggatt ttgcattttt tggctggact attctcagtc catctagttg
    12601 gaacgcttct tttacttgtt ctacaaatgc ctctatagaa atttcttgcg gcaattcacc
    12661 tactcgtcct aacccaaaca tttcgactgg ttcatctata gcgaaaagat cataagcagg
    12721 ttcctcataa ggatgagctg accgcatggc ttgaatcact tgtttttcga tagtttccgg
    12781 caaaataacc tcaacttttg cctcttgaac ttgttctgtc ttccctactt tcccaatcgc
    12841 tggttgtgca ccagcttccg gagtaaatcg accgtgtcca atcgaggtaa aacttgtacc
    12901 ggtataatca ccttgtgtac cagcaccagc tgcagctaat acttcacgca ttttctgtgc
    12961 atgatccaca ggtacataaa cggccagttt tttataatga atctcatgag tctttactaa
    13021 ataactctcc acttcaatac cgagaagttc acaaaaccag tcattcagtc catcccaaat
    13081 aatatccatg ttcgtgtgtg ctgcatagac ggcaatatca tgtttcaaca aatctgcata
    13141 cattttttct tgtggctgat cagtaaccag ccgttttaca ggacgaaaga ttggaggatg
    13201 tttagcaatc agcaaatcga tttttttctt gattgcttct tcgaccactt ctggacgaac
    13261 gtctaatgtc atcattactc gttggattgg cttatccaat gttccgatat gcagtccgac
    13321 tgggtctcct tcttcagcta gccactgagg acaatagctt tcaaactttt ggatgaaagt
    13381 gcgtccgctg attgtcatgg ctttaaaacc tcctcaatca tttggatttc ctgctcgatt
    13441 tgtttttgac gtgcctcttg attagctgct tgtttgagct gttcacggat agcttttctt
    13501 tgttgaagct ctcttgtcca ttttgatttg aagatctcac ttttctcttc aagcagaaat
    13561 ggcccgaaca tcagctccat ttcattgtaa gtaactggtt tttgttcttt ttcggctaca
    13621 atgatttcat atattttttt attttcttca agtatttttt ctgattggat tttatactga
    13681 ttttcttgca gccaagatcg taatgtacgc tctccgatgt ttggctgaag gatcaaacgt
    13741 tcttgtccgc ttaagcgttg attcaacttg ccagcttcta ggatatctcg gatcagtgaa
    13801 cctcccatac ccgcaatcgt gacagctgta ataccatctt gtggctggat agcatctaga
    13861 ccatttgcaa ggcgtacggt gatacggtct tctaatccat cttttttgac ttgcttacgt
    13921 gctgattcat aaggaccttg aacgacttca ccagcaactg catattcgat ttttcctctt
    13981 aatatcaatg caacgggtaa gtaagcgtga tctgatccaa tgtctgctaa acgagcggat
    14041 tcagggacaa agtcacccac tgcggctaag cgctgagata aatctaaatg gttcatagct
    14101 tcactccttc tagataatta tagcattttc caaataaaat aaggagacta ggaataaaaa
    14161 aattcccata tctccttaaa aaatatacct tttcgttcta taaaacaaca ttttcttatt
    14221 tttgaaaaaa tgttattggt tatttgttca atgaaacatc aataatcgaa tctccaggaa
    14281 cttgcggtgg gatagacatc gtcatgatcg gcatctcaac taatgtaaat tggactttgt
    14341 caccgatttt caattcattt atgtccacgc ttattttttc agcagtagca tttaagatca
    14401 ctccgtcatt gctgaatgac gttcccatat tcgctggatc ttctttggcc atgacatcga
    14461 cgagtttcaa ctgaagcgtt tcttcttctg cttctacttg agtaatactt tcaatcgttc
    14521 cgacaaatat gctttgtctc tccgttttcg tactgcttgt ttttactgtt tgacccgcat
    14581 caatcgaatc aacagaacct gtgttgctat ctgcgctacc gcagcctgct aaaagaaaaa
    14641 atagaccaac tacagatata ctagaaaaaa tagccctcat aaaaaatcct ccgtttaagt
    14701 ttctgcatta cttgttgcgg tgattgtctc cggtcatttc tgtccatata atgattcgct
    14761 gacctgcttt tactactagg tcaaatgtca gttggacttg tcaactgttc aaccattgcg
    14821 tcggttgcga ccagtcattt cgatttcttt tgtcaaataa cgtatgcgtt ccattatccg
    14881 tttcgctttt agcggctctt catctccacg ttgagtgacg gacaaatgct gttccaattc
    14941 atccattgta aaattcgatg agaagaaggt tggtaactgt tcctgcatcc gatattgaag
    15001 gataacaccg aatacttcgt ctcgtatcca actactcatc gcatctgcac caatgtcatc
    15061 gatcatcaaa ataggtgaac gtttcactgc atctaacttt tctgccactt gatcttttcc
    15121 gattgcctgt ttcatctcaa cggcaaatgt cggaaaatgg actagcgtcg tggtgaatcc
    15181 agcaatcgct aattctctcg ctgttgctcc taataaatat gtttttccaa taccaaaact
    15241 tcctactaga tacagtcctt tatgataccc ttttggatca gcagtatatt tctctacaaa
    15301 atctagggct tctaagctcg catatcctcg tccatctgta cgttcaaagt tttcaaaaga
    15361 agccgattga atatcttttg gcatatccat tgcttttact cggcttctga tttcttcttg
    15421 tctttgccgt gctaacaatg cttccgttgg tacataagtg acatccacat aatgaaaatt
    15481 caacgtcagc ttcggctcat agccgggagc gatcatcgtt ggatcgtttt tttgatattt
    15541 acgcttttct tgcacgaatt catacaattt agcgtaactt ttttcaatat ccgcttgtgt
    15601 caattcttct tggtgtgcgc ttagaaaagc ttgaacatcc gcatcattta agacttcagc
    15661 gatcatttca gcatactttt cttgataatt gcgtttgctg atgatcctct tcaattcttt
    15721 gcctacgtct tccactagat atcaccctcc ttttttgcca ggtattcttt aaaacggcgg
    15781 tcaatctctg cttttttctc gctgctgatc tgtttttcat cttttggctg gttgacccaa
    15841 tcaggaagct tttcccgtcg aacatttcgc ttgccagttt gataatttgt ttgcttcttt
    15901 tgttttgatt gttttgcttg attacttatt tgccgaatgt gctggatcgc ttcttctgct
    15961 gtggcgattt tcttttgtgc ccattcattt gccactgtat ttacataact ggcatttaag
    16021 ctagcattat tcttgatcac tagtacataa ttcagcaaaa cattgatcac gctactggaa
    16081 agacctgatt gagaaacaag atcttgcagt aaccagtttt cttgtttaga aacatatccg
    16141 cctttttctg acttgattgc ttccagatat ttcatcggtg gaatgctctg tgcttctttt
    16201 atcagctgaa gcgctgcgtt tgtcaaatgt tcgtttgacg agggttcatt actgttttca
    16261 gaaaccgcaa ctgtttcttt tgtttgcttc accggacgtc tctgcattgc caaaatggct
    16321 ttttgcagtt ctttttcaga tactttttgt tcactaaagt cataagattg actgatcagt
    16381 tcgccaagtg tcaattcatc gaaaccgtaa agctgatgat acatgtcgac ttgtttgatg
    16441 attggatcat caagacttgc aatatgtttt tttgccagcc aatcattcaa aaactgccaa
    16501 tcgaatgtcg aatctgcttc aagtaatgct actgattcag attcttcaaa agatttattc
    16561 agcgattcga tgttttcttg attagcagcg tacactgctt cattgaagcg ataaacatct
    16621 aaaaattttt ttgtgatttt ttgatatccc tctgctgtat gccttttagg ttcaaagaat
    16681 tgggtaagct tctggaatcg tcgttcacca actttttcca gaagaagaaa agccagaaca
    16741 gagtctttga aaaatgcttt aggaatgagt ggttctaaca gctcataaat aaaacgaggt
    16801 cccagatcac tatcttcttt aaaataaaca tccagtaagc cgattccttc tagctttttt
    16861 cttgcatcat aaaattgcgg gataccgcag cctagactac taagcagatc tgcatgcatc
    16921 ccttcaggac ctttgccaga ttcttgagag atttctgata acagggtgaa atagatactc
    16981 aaggcttctc cgccgattag tggctgataa agatacagca aggcgttctt gctgtcttct
    17041 ctcagcggag ccgcttttct tccttggtaa atattttttg gttgaagctc tttccaagca
    17101 ttttccaagg aaatgcctcc tttttgtttc ttaattgttt tctttattat tctctttatt
    17161 attttctttt gctttatcca cgatatcttg caactctttt aagaaaacag acatatcttt
    17221 gaactggcga taaacacttg caaaacgaat ataagcgatt tcgtctaaat caactaaatc
    17281 ttccatcaca tattcaccaa ttagattcgt cgatacttcg ttttctccga gactgcgtac
    17341 gcggttttcg acatggttga cgatttgttc catttgttcc atagctacag gtcttttttc
    17401 tgctgaacgg atgagtcctc ttaaaatttt atcgcgattg aactcttcgc gttccccgtt
    17461 tttcttgatt actaataacg gagcagcttc aattcgttca aatgtagtga atcggaacga
    17521 acaattctca cattcgcgac ggcgacggat cgcacgtcca tcatctgctt gacggctgtc
    17581 gattactcgt gaattattat gttgacatct gggacatctc attttgtctc acctcttgca
    17641 tcgcatttta tataaaggat gtgtctttct ttacaactag ttgtattata acacgaaaca
    17701 aatcacaaac gcgactgtcc tacccaaaaa acacggatat aagaacaatt ttttctactt
    17761 tcatgcgtaa ttgtgtcccc tgtccttact aaatttcaat tttccaatac tgatcacctt
    17821 gattactgag aaataactta cacaaaagct gatttagtca tctagcacaa aaaacttgaa
    17881 gcagatgaac accttctcat ctgcttcaag tcacatctcg attttttact attttataac
    17941 ttctcttcga ttcctgtata gtgtttcatt cgctgttcct cgattttttg ctcatcggga
    18001 atgtggtcac tttctgggag cggtgtttta ctacgatcag gtgtcggatt ttttcgttct
    18061 tgagtcaaat cttgccgctt cgcttcttct tctgcctgct ggtcttctat ttcatcgatt
    18121 cttgcttggt actgcttttc tttttcttct gctgtttctg attgcgtacg accttcatcg
    18181 attagatttt ctttattgag catctcgccg attttttctt ttgcgttacc tacgacttta
    18241 tccttttgat tttttaatga tcccatcgct atccctcact ttctatatac tcaggatagc
    18301 aaaattgaca cagatactca aagaaagcga ttagcaaaaa gccaattttc gatttgtccc
    18361 acaagttttt cttttgttcc attgttgtcg aacaaaatat ccgcacgctg ctttttttct
    18421 tcgattggaa gttgactact aattctctgc tgcgcttgtt ctagcgtcag ttgattacgt
    18481 ttcatcaaac gctgaagctg agtttctggc gtagtataaa cgacagctac tgcatccata
    18541 tagtggtcat aatgaccttc gtagagcaat gggatatcta caattactaa aggatgattg
    18601 gaacttagag aggcgatttg atcttttatc gcttttcgga taaacggatc taatagctga
    18661 tcaagttttt gccgtttttc ctttgatgga aaaacaatca tcccaagttt ccctcgatct
    18721 agttgcccat caggctgcaa tacctcttgg ccaaacactg atacaatttt cctcagagca
    18781 agcgttccag gctcaacagt ttctctggca atcacatcgc catcgatcaa aggaaatcca
    18841 tgagactgaa agactttggc tgccgtactt ttaccggtag ctattccacc cgtcaaacct
    18901 aaaacaaatc ctactttgcg atttttagcc tttgacaatt cggacaatag tgcgtccccc
    18961 tttgtgccac ttttgttttc acgatcggcg ttccacaacg tatacatggt ttgccagttt
    19021 gctgatagac atgcagtgct acttgaaatc cgcctgattc tcctaatgca ttcaaataac
    19081 tgcgaatcgt ggtacctccg gcttctattg cacgatccaa aacatcaatg atcgatagac
    19141 gtaattgttc tacctctttg gggcgcagtg tgttcgccgg ttgttctgga tggatcttcg
    19201 cttcccataa tgcttcatct acataaatat tacctagacc agtcactaga cgttggtcta
    19261 gcagcaatgg cttgattgct ttgtgggact ttttcagtcc atttgcgaaa tctgctaata
    19321 agaagctatc tggcaaaggc tcaggtccca atttcatgat tcctcggtaa gttgcacttg
    19381 ctcctttttc gacgattgtc atacgaccaa attttcgtac atcgttgtag cgaagttggc
    19441 ttccatcctc aaaaaagaaa agtacatgcg tatgtttatc gattggtgta ttttctttcg
    19501 taaattgata ttttccttcc attcgcaagt gtgagatcag ttcacaatga tcgagatgaa
    19561 aaattaaaaa cttccctcta cgtccaatgg attggatggt ttcgccaaca agagaagcct
    19621 caaagatggg tgtctctggt tgctcgatga tcttgggcca caagacctga actttttgaa
    19681 tcgtttttcc tactactaac cgttccaatc ctttacgaac ggtttctact tctggtaatt
    19741 ctggcatttt ttcttttcac cttcattata ttgctctgtc ttaaacaatg tttattttgc
    19801 ttcgtaccac gtttttcccc aactgctgtc agtgatcagt ggtacatgaa gagaaacagc
    19861 ttgttccata acttccttca ccaatttatc taatgattca agttcttttt ccggtacctc
    19921 gaagacaagt tcatcatgaa cttgtaacag catcgtagct tgaagacctg tttcttttaa
    19981 tcgtttatcc aattcgatca tcgcgatttt caaaatatca gcagcacttc cttggattgg
    20041 tgtattgata gctgttcgtt ccgcaaaaga acggatattg taatttcttg aattgatgtc
    20101 tggcagatat cgacggcggt gatacagcgt ttcgacgtag ccttggtctt ttgcttcgcg
    20161 tacgattctt tccatatatt ctttgacacc tgggtatttt tcaaagtacg tatcgatgta
    20221 ttgctgtgct gcttttcggc tgattcctag attttgtgaa agtccgtaat cagagatgcc
    20281 atacacgata ccaaaattca ctgcttttgc ctgcctacgc atatttggtg tgacatcctc
    20341 tgccttttcg attccaaata cacgcatcgc tgtgctggca tggatatctt gaccttctag
    20401 gaaggcagct ttcaaatgtt catcatttga gatatgcgca agtacgcgta actcgatttg
    20461 tgaatagtca gaagaataaa tcagccagtc tttttctcta ggaacaaatg cctgacgtat
    20521 ctttctccct tcatctaatc gaatcgggat gttctgcaaa ttaggatcaa cagagcttaa
    20581 tcgtcctgtt tgggtcaatg tctggacata acgggtatgc acttttccat ctgattggat
    20641 cactttcagc agtccctcga cataggtaga ttgaattttt gcaatttgac gataggtgag
    20701 gatatcctct acgattggtg cttgttcacg aagctgttcc agtacatcta cggctgtgga
    20761 atatccagtt tttgtttttt tgataacggg taatcccatt ttttcaaata agatcactcc
    20821 taattgcttt ggtgaattca aattgaattc ttcgccggct tcttcgtaga ttttttgttc
    20881 gatctctctt aagcgagcag agaactcgcc cttcatttct tgtaagcgag tcgcatctac
    20941 tttgatcccg cttatttcca tctctgccaa aatgatagac aatggcagtt ccattttccg
    21001 gaaaagatct tcttggtttt tttctgccag ttcttggctc aattttggtg tcaacgcatt
    21061 gattgcagct acttttcgcg ctaaatgagc aaaaaagagt tcctcttctt ctggtaaacc
    21121 ttttttcgcc cctttgccgt atacagcttc atctgattgg atatcgttgt atccgtaatg
    21181 agcagctatt ccttcgatat ctgtgctttt ttcattcgta tcgagaaggt atgcacctag
    21241 caagacatca aaatcgattc ctttcgtttt tccagtatag cggtttaaag caacataagt
    21301 tcgtttcgca tcatatactt ttttcagccg agtcggatca gtgatccaag aatggaacgc
    21361 tcggttttcg aatacagata gatcgttcgt gacataaatt ttcttttctg tcccccacgc
    21421 tactccgacg atttcctctg tatgataatt gtcgcccatc atttccacgt acaacgccat
    21481 atccgtagta aacatttcct ctttgaattc atgaacgact tcaaacaaaa tatcatccat
    21541 ttttacttct tcttctacga tattcaattt agaaaggaac tgattgaaat ccatttcttt
    21601 ataaaacgga acgagtttat cgagattttt cccttcatac ttcaatgagt cgatagagac
    21661 atccacaggc gctgaagtat tgatggtcgc caattttttc gataagaaag cctgctcttt
    21721 gtcatttatc agattttctt tcattttgct ttgcttcatt tcatcgatat gttcataaat
    21781 tccttcgact gagccatatt gtttcaatag cttgatagct gttttttcac cgattttagt
    21841 cacaccgggg atattgtcag aggcatctcc ggctagtccc ttcatgtcaa taatctgttt
    21901 tggcgtcaac ccatcatatt tttccgctac atgttctggt gtatagcttt cgatatcgct
    21961 gacacctttt acagtgatat ccacttttac ttctttacta gctaattgag tcaaatcccg
    22021 atcacctgaa agcacgacga catcaaattg atctttgtct actttttctg ccaaagtacc
    22081 gataatatca tctgcttcat aattgggcaa ttcgtaatac tgtaccccta gtcctgtcaa
    22141 caaatcacgt atatatggca tttgttcttt gaattcgcca ggtgttttgg atcttccacc
    22201 tttgtactcc gcatacattt ctgtccgaaa agtcgttttt cctgcatcga acgcaaccag
    22261 aacatgagtc ggttgctctt tttgcataac attttcaaac atcgtattga atgcatagat
    22321 tgcatttgta tgaagaccgt ttctattttt gaaacgttca agagaattgt gaagcgcaaa
    22381 gaatgcgcga aaagccacac tgttcccatc aacgagtaat aatttatttt ttgtcatctt
    22441 cttaactcct tactgtaaag ttcttattca ttctaacaaa gaatcctgcg gaatgcgaaa
    22501 agaaaaagcg ggaccatacc aaataattcg gtatagcccc aactgatgaa tttttttctt
    22561 ttttagttgt tccgtccaaa gattctcaga aacgccaaga acaagttgat aaagtccaaa
    22621 tacagcttca atgccatgaa tacagcaatt ccactatgtg ctgttccgtt tgtctggaca
    22681 tagatattac ggatgctttg gttatcataa gctgtaatcc ctgcaaagat gacgactaac
    22741 agtagggaga tgaacagctc aactacgcta cttctcaaaa tgaagatgtt caagactagt
    22801 gcaatgatca ctccgattaa agctgcctgt cctgctcttc caaccccgct aagatctctt
    22861 tttgttgtta ctccgacaac agacatcccc atgaacatca aagtagctgt caagaaagct
    22921 tgcgttactg ttccaatatc gtagtatgca agagtaaccg ctaatgttat gccattcaaa
    22981 agagagtagg caataaatcc accgattgct aagctcggat tcttttgtgc ttttacgcct
    23041 agaaccatta ctaaaatcag ttcaacgatc cacagaccca caaagcctag tggaaaatta
    23101 ttgataaaag ttgctaattg caaaggaaat acccaaagag ccaagtaaga agtgactgca
    23161 ctaagggcaa gtcccatacc taaaaatcca taaacttttg cataaaaacg attgatacct
    23221 gacattgatg tacttggatt attcattcaa ttcaccttca ctttcgattg ctatttccaa
    23281 ttcaggaact tgtggcggtt ggaccataac gacagcatcc aacacacgat cttcgttcgt
    23341 attctccgct tctacagaaa tcgtcaccac atccttcatc ttatctactt ttatgacctc
    23401 aaactgaaaa gtcagtgttt cataatggaa aaccggttca ataaaattga cagaaaaatt
    23461 gacaacatgt gatcctggtc caggaagatg ctttgaaatt gcacttgtga tgatccccat
    23521 cagcatcact gatggaacga ttggctgttg atattctgtt ttttgtgcaa agtcatgttg
    23581 gatatacaaa ggatttgcat cattcgtcaa tcccaaatac agcaaaagct ggttatcttc
    23641 aatagattct gttaaggata acgagtcgcc ttcctcgatt tcatttattg tttttcctag
    23701 ttttctcggc ttacctatgt tcaagataga aaccctccag tttattttta atcatacatt
    23761 attgtaccaa aagtagcgta aaaggcaagt tctgctccct tgatttaaca gattcctata
    23821 gtttacagag gtcgtgaaaa agctgtcctg catcaaataa taagaacagc caagaaaaaa
    23881 cagcttctca tgtttttgat ttgattgggc ttattaccgc agatgcaagc ttttgaacac
    23941 cgtttatcat aagttgcaaa aaagggggct tggcaagatt tttgccactc cccctctttc
    24001 tatttttata aggacactgc agtaattgta ttacagtaca tttttcgtcg tgctgcttaa
    24061 aagtttttca tacgcttgtt caaatttttg aacatctcca gcacccatga agattactac
    24121 tgcatcatga taatctaaca atggagatac attattttct ttgatgactt ctccaccttt
    24181 tttgattttc gctccaaggt cttcaatctt aacgtttcct tgttcttccc gagccgaacc
    24241 aaaaatatcg cataagtaaa ctttatcagc taaatcaagt gcttctgcaa attcatccat
    24301 caatgcaatc gtacgcgtaa acgtatgggg ttggaaaact gcaattattt ctttatctgg
    24361 atatttttgg cgtgcgccat caatcgttgc tttgatctct gcagggtgat gcgcataatc
    24421 atcgactacg gtcatgtctg ctacgatctt ttcactgaat cgacgtttta ctcctgggaa
    24481 agtcagcatt tcttctgcaa cttctttcag atccagtttt tcgaaataag ctactgcaat
    24541 aacacctaaa gcatttaaga tattgtgttt accgaatgct ggtacagtga agtgcccaac
    24601 aaattcatcc ccatgataga catcaaaagc agaaccagaa gttgtgcgtt cgatgtttcg
    24661 tgcttggata tcatcgtttt ctgtgacacc ataataataa atcggcacat ttgcttttaa
    24721 tttgcgtaaa taagcatcgt caccatacgc aaaaatagct tttttcactt gtccagccat
    24781 tgtttggaaa gctgtaaata catcgtcgat acttgtataa taatccggat gatcaaaatc
    24841 gatattggtc atgattgcat agtctggtga ataggcaagg aaatgacggc gatattcaca
    24901 ggcctcaaat gaaaagaatt ccgcttgcgg atcaccatgt cctgttccat ctccaattag
    24961 ataacttgtt ggacggacac cactcaatac atgagagagc agtccagtcg tacttgtttt
    25021 tccgtgagag cctgttacag cgatactcgt ataattttgg ataaaatgac caataaagtc
    25081 gtgatagcga atcacttcca aacctaattc ttttgctcgc tggatttctt catgtgaatc
    25141 aggaaatgca tttcctgcaa tgatcgtcat ccctggtttt acattatctg cgttaaatgg
    25201 cagaatcgtg atatttgctt tttctaaatc tctttgtgta aagaaatatt tttcaatgtc
    25261 tgatccttgg acatttagac cttgttcatg taaaacaaga gccaaggagc tcattcctga
    25321 acctttgata ccaacaaaat gatacaattt attttgattt tccatttgaa tcctcttctc
    25381 ttatctagcg tccattcttt tagctattac attcaacacg ctcctcatta tcttataaac
    25441 gattgaaaat ttcaaccaaa tttatttgga aaggaggatt cgcttgctaa aatggtcaag
    25501 tacgagaaaa atccgttttt attgactctt taattctggt gtaagaagac caaacttcct
    25561 ctgatatatc aatgtgagga ataaaatctt ttcctttcgg accatagccg ttctcatcgt
    25621 catggagacg ttccatttct cctaatacta gcgcatggaa atattcctta ggccattgat
    25681 gattcagttc ttttgctgta aagatgatct cttcagaaaa gtgaaggatt tccggcacaa
    25741 atggcacagc attcgcgaaa atagtttgtt cattttgcca tactttctca aaagttgctc
    25801 gagtcctgcg ttgcagtgtc ggatcattca tcaataagat tttttctggt acagctccta
    25861 aggaatgaag aatctccagt gaaaaagtgg cgttctctcc agaatttgta gacttcgatt
    25921 ctatcagaaa agccttatcg ggcaaatcat agacttcttt tagatactga cgacagattt
    25981 cactttcact catgccatct tcaaaatgaa atccttgctt ttcaaaattc tcataaagga
    26041 ttctagtcgc atgccccacc cctccgacca aaaacaattg atctacttgg ccatttaaat
    26101 acatttgtgc tgctttatca gctaagatag gaagacagtt tccagctaaa ataagtgtag
    26161 gtgccgtcag ttcctcaaaa tttccctctt caggtgcggc taaaaaattt aatagctgat
    26221 tccagtcttt tagattcctc tgctcacttt tttccaaagg ccttctatcc tttcatctga
    26281 ttttttgtgc ttcattaaag tcttctcttt ttgtctcaat ccatttgatt tctccatgac
    26341 atcttccaca acgatatttg cgtacatcga ttcttctctt gcggcagatc gtggcagaac
    26401 aatcttggca tcgataatac tccatcttta tctgtttctt cgtcaaggct ggtgcatagc
    26461 gcaaccctcc tgtttttttc aaaagatact tgaaatccat atctttatgc tgataccctc
    26521 ttccagctag atgaagatga taatggcaaa gttcatgttt gatgattcct gcaatcgtct
    26581 cttcgtctga ctcttcaaat agcttcgcat taaaatctaa atgatgatcg tttaaatgat
    26641 agcgcccgcc agttgtacgt aagcgtggat tgaacattgc ttggtgcttg aaaggaagct
    26701 ggaagctttt taatgatatc tcttctacta actgctgcag ttcttcttgt gtcaattttt
    26761 gttactcctc attgttactc ttcataaggg gaaagcatcg tcaaactgat tctgcctttt
    26821 ttcgtatcga cttgttctat ccaaactgtt acgatatctc ctactgacac gacatctgta
    26881 ggatgcttga cgaatttttt gctgagtttc gatatatgga caagtccgtc ttgcttcaca
    26941 ccgatatcaa caaatgcgcc gaagtcaatg acattacgga ccgttccagt caattccatc
    27001 cctggtttca agtcttccat actcaataca tctgtacgta ataagggcgc tggcatctca
    27061 tcacgcatat ctcgtccagg ttgtgtcagc ccagctaaaa tatcggctaa cgtttcttct
    27121 ccgactgata ggctttcagc cagtttctct acggataatc gtgtcaattt ttctactgct
    27181 tcttctgtac caagctcttt ttctgaaagt tggactgaag ttaatatttc ttttgcaata
    27241 gaataacttt cgggatggat acctgtgttg tccaaaatgt ttttcccacc agggactcgc
    27301 aagaatccga ttgcttgttc atatgctttt ggtcctaagc gtggcacttt tttcaattga
    27361 gttcgtgcgg tgaattctcc gttttcttca cgataaatga caatgttttg ggcagttgtt
    27421 ttattcaatc ctgatatatg ctgcaagagc tgcgggcttg ctgtattcac atccacacct
    27481 acttggttaa ccgctgtttc cacaacaaaa tccaattgtt cagccaaacg tttttgagaa
    27541 acgtcatgtt ggtactggcc tactccgaca gctttaggat cgattttgac taattcagct
    27601 aaaggatctt gcaggcgacg ggcaatagaa acagcacttc tttcttcaac ttgtagatgc
    27661 ggaaattctt ttcgtgcaat ttcgctagcg gagtaaacag aagcgcctgc ttcgttgacg
    27721 atagcgtaat aggttttatg gtcggcggat ttcaactgct cagccacaaa aagttcagat
    27781 tcccgactgg ctgtaccatt tccgatagct accatatcta cttgatattt attaattaat
    27841 tggataaaag ctggtccagc agcttctctt tttgcttgcg ctgcaggttt atgaggataa
    27901 ataacttcga ttgctaaaac tttccctgtc gcatctacga ctgctagttt acatccagta
    27961 cgataagccg gatcaaatcc caggacaact ttacctttta gtggcggttg caacaaaaga
    28021 ttacggaggt tttcgccgaa aatagcgatt gcttgctcat ctgccttttc agtcaattca
    28081 ttgcgcagtt ctcgctcgat tgctggctga ataaagcgtt tataactatc ctgataggct
    28141 tctctgacga atgaagaaga aggagaggca ggatttttga ctatttggcg atccaagtaa
    28201 gctaaaattg ctgcttcatc tacttgaaag aatactttca gaatatcttc tttttctcca
    28261 cgattagtag ctaatattcg atgagaaacc attttatgga cgggttctgc aaagtcataa
    28321 tacatttcat atacgccttt ttcatctgct tgctcatcct tgacattgct gacatacata
    28381 cctttgttat acgtataaga ccgaatccaa gtccgaaatt ttgcattatc gctgacttgt
    28441 tcagcaataa tctcatgagc accgtgcaac gcttcttcgg cagaagagac ttctttttct
    28501 ttgtcaatat atttttctgc ttcagattgc acctctccat ctgtaagctg catcagccac
    28561 ttcgcgagtg gttcaagtcc tttttcttta gcaatcgttg cttttgtccg tcgtttttgt
    28621 ttgtatggac gatacaaatc ttccacttgt tgcattttca cggcttgggt gatttctgct
    28681 tctaactcgg gagtcagttt cccttgttca tcgattagcc gaatcacttc agttttacgt
    28741 ttttctaagt tttcaagata ggcataccgc tcttcgattt cacgaatctg gacttcatca
    28801 agacttccag tcatttcttt tcggtaacgc gcgataaaag gaacagtgtt tccttcattt
    28861 aacaaattaa gtacagtagt caactgctta ggacgatagt ctgaaagttc tttttgtaca
    28921 agctgaatga tcgtctggtt caaattttcc gtcattaaaa tgcctctttt ctgctaaaaa
    28981 ttgcttcttt attctatcat actttttatc ctgggttaaa ctactgaaag aaacaatatt
    29041 ctctataaaa ataaaaagag ccgcaacaaa gtcacagctc cttcattctt tccattagtc
    29101 agattccatg ccaaggtcct tcgttgttct gatattcaat atcttgaaga ataccttcct
    29161 tgtcaaaaac aaccccatgc aatgaccctc cgaatacagc cccaccgtcc attccgattt
    29221 tatgatcctg aatccagagg tctgttgtct gcatatctcc atggagcatt ggtgtaatcg
    29281 tatgtccaaa tacaattgtc ttacctgtat tgtttttccc ttcatgaaat ggttccctta
    29341 tccaaataaa gtctcttaga cttgtcttac gccagtcttt ctttgtcaaa tccacaccgg
    29401 catggacaaa aagataattt tcccattcat aatacaaggg gcgctggact aaaaatttaa
    29461 tcagttctga atatctcgat tggatcatca tcgagatttc agttggggag tattcctccg
    29521 atgcccctgg gtgaatcagg ctttcaaacg tttcttttcc accatttctc atataaggcg
    29581 cataccagtc ctccggtgat tgcagaaatt gcaaaaaata ttcttcatgg tttccccgga
    29641 ggtaaatggc tcctgtttct tcgactaatt gctttcctaa taaaaaacat tcttttgtac
    29701 gaggacctcg atcattgaga tcccctaata aaacaagctg atggatcttt ggatcataat
    29761 agtgaataag tttttcaaaa agctcatgtt tgccatggac atccccaata gcataaatca
    29821 atgatttcat gtggatcacc atcctcaacg ctagtatagc gcatattgga aatggtcgac
    29881 aatcaaaact attgttttct atttaattaa agctgtaaac cgcagttctg cttcttctgg
    29941 agagattcca tggtcttgaa catagcgatt gcgcggatgt ttcgcacact ctatgctaca
    30001 tgctcctaaa tgtttttctt cattttcaat ggaagcaaga atctgacgat tgcactcagg
    30061 attgctacaa ttgatatatc gttcacatgg tgttccgtca aaccagtcgc gaccgacgat
    30121 ttgtttatct acttggttga tatcgacagc gatccgttca tcaaacacat acatcttgcc
    30181 gtcccataat tctccttgga cttcaggatc cttaccataa gtagcaatac cgccatgcag
    30241 ctgtccgaca tctttgaatc cttcacggac aagccaacca gaaaacttct cgcaacgaat
    30301 gccaccagta cagtaggtca ctacccgttt ttccataaat tcttctttgt gctcgcggat
    30361 ccagtctgga agctctcgaa aactgcgtat atctgggcgt acagcccctc gaaaatgtcc
    30421 caagtcatat tcataatcat tccgtgcatc aatcaccact gtctgttcat ctaaaatagc
    30481 ttctttaaat tcttgcggtg aaagataggc acctgtcact tggtttggat caatgtcctc
    30541 ttctagcttt aaacttacaa gctctggacg gtatcttaca aacatcttct taaaggcatg
    30601 tccttcgctt ggatcgatct tgaacatcgt atcagcaaat cgaggatcag ctttcattgc
    30661 tttcatatag gcagtcgtgt tttcaacggt tccagacaca gtaccattta ttccttcttc
    30721 agctactaga atacgacctt taagatccaa agatttgcaa aaggctaaat gatttttggc
    30781 aaattcctct ggttcttcga tttttgtata attgtaataa agcagtactt gataatccat
    30841 gtatttcttc cttccttctt ttcgttccta aacagtataa agaaggcgtt cgcatttgac
    30901 gatttatttg cttgtgtata attacgcatg gaaagttttt tgtttacata atattattat
    30961 ttgaattaaa aagacttcgc tttcctgttt cgcacagaaa aacaaagtct ttatttctat
    31021 tatttacctg taaccgcaaa gtagttgcca tcgggatcag caaagttaaa gacatattct
    31081 tctccaagct gtaccatatc acctaatgtg actgattgcg tctgcatttt tttatatagt
    31141 ccaaagatat ctttactgta aaacatcaaa gatggggcac tagtagctgt gccaggtgaa
    31201 tgggactcaa caaatgctcg atcatataac agaaatgagg tttgactgtc ctcaactcct
    31261 acttcaatta taagcgtccc atccatttct tgacgatctt tttccgcaaa gccaactgct
    31321 ctccaaaacg ttgcattttt ttctacatcg tccacataaa gcatgatttt tatttcattc
    31381 gtaaacatct gtttcctcct aaacgatctg ttctaacatt ctcaactttg tcagtttacc
    31441 ataggaaaat gggaaaatct acgaaagctc tcaacagaat cgtcctactt aaatacgctt
    31501 ttctttaata ataaataagt tggttgtttt tgttatttgc gctttcactt ttcggaataa
    31561 ataatgtgta tttatatgga ataaataaaa aaagaggtcg tgactaaaaa caagtcacaa
    31621 cctctggtaa acggtgttct cctgacaact cctcggtcac aagacacaat attcaacaaa
    31681 catgagaagc tattttttga aattgctctt tgtgactcag agtgaaacgg caggctcacg
    31741 acctctttct taaacggtgt tcaaaagctt gcatctgcgg taataagctc aaccaaacgt
    31801 aaaatatgag gaactatttt tgtttggctg ttcttattac ttgatgcagg gcagcttttt
    31861 cacaacctct tattcacggt gtttcagaag tagcttctcg gaaataagcc gaaatttcac
    31921 aaaaatttga gaaactattt ttaggaaact tcttcttatt tcttaaagct gaccacttct
    31981 atcacaacct ctttttgact atgcattcac atcgatcact ttagaatcat cattgatgac
    32041 atcgcgatct aaatctttga tgcgtgcact tgtcacgata ccagctacca tgctaccgct
    32101 gacgtttact gctgtacgtg ccatatcgat taatggttca acagagataa ctaacccaac
    32161 gattgctact ggcaagttca ttgcccctaa aacgatcagt gaagcaaatg ttgcgccccc
    32221 accgactcca gctacaccaa aagaactgat tgttacaatt gccacaagca tcaaaataaa
    32281 ttccaaacta aagacattca ctccagctgc tggtgcgacg atcgttgcca acatagctgg
    32341 ataaacgcca gcacagccgt tttgtccaat agacaaacca aaacttgctg agaaattagc
    32401 tgttgcatca tctactccta aagcttttgt ctgagtctcg atattcaatg gcaaagctcc
    32461 agcacttgaa cgtgaagtaa acgcaaagct caataccgga aaggcttttt tgaaatagtt
    32521 gattggattg acttttgcac caatcaaaat cagtgagtgg acaatcaaaa cagcaagcaa
    32581 tgctacgtaa gaagcaacga caaatttacc taaattgacg atagctgcaa aatcacttgt
    32641 tgccaaaacg tttgtcatca aagcaaatac tccataaggt gtcaaacgca aaacaagcgt
    32701 cacgatccgc ataatgattt tatacagact gtcgactaaa tctgaaaaga atgctgcttc
    32761 ttttggcgct tttcttttca cgcctagata agcgattccg acaaatgccg cgaaaataac
    32821 gactccaatc gtacttgttg cacgactacc tgctaaatca gcaaaaacgt ttttagggat
    32881 aaaggataaa atctgctgtg gaatcgataa gttttcaact gtttcctgac gtgttgccag
    32941 ttcagagatt cgtgctgttt ctgcagctcc ttgtgtgaat ttagctcctt ggagtccgaa
    33001 cagcattgtt gaaccaattc cgatcaatgc tgcaatagct gttgttccaa gtaaagaagt
    33061 caatacagtc gcactaattt tccctagctt ttcagaaact ttcatttttg tgaatgcacc
    33121 aatgatcgaa ataaacacta aaggcataac tagcatttgc aagaatgata catatccttg
    33181 acctacaatc ccgatccatt ccattgattg agtaacgact ttgtcgtcta caccaaagat
    33241 caattgaagc agaccgccaa agacgatacc aactcctaat gctgtaaaga cgcgtgttga
    33301 aaattttgta tggcgctttt gcatacgata gaacacatat aataatgccg caaaaactag
    33361 aaccactaaa actgtgatga gtgttgtcat ttttctcctc ctcatattat taatgacaag
    33421 tccatcgaag gaagtaaagg ttcccgagaa tgattcttca accatcctcc tgaaaaaatt
    33481 tttttcagtg acttttatat tttagacgat ttttatcgaa atgacattct ttttgcttac
    33541 aattagtaat aaaaaaacat acctgtgata gctttaatct atcgcaggta tgtttttact
    33601 gtctattctt gtttttctga ttgaaaattc aattttaccc caggctgatc taaggcgatc
    33661 tctgtcacct cataagacat tcggcgtacg atatcgaata acggtgctac tagctcgtcc
    33721 acttcttctt gtgtgacatc attttgacct tcaattttcc gattgaggat atgattgatc
    33781 tgactcacac ttccagataa aacatatgta tcaaacacta aacggaactc taagcgtgct
    33841 cctacgatgc tatgttcttt cggatagttt tcgatcgttt cttttaaagg cgagaagcct
    33901 actttcaaat ctgtttctac ttcttggttc ggcaatctca tatcataatg aaatgcttct
    33961 acaacttctt tttgtcttct gatttccata taattttatc actcctttat gaaaattata
    34021 acatatctcc tttgaaaggg gatactactt tttaatgatg ctctgcaagg taaggatttc
    34081 attcagattt cctaccacat aatctgccgg gatctcaccc agaaaatgat cgatgtcgta
    34141 aagacaagta gcgactcctg catttttccc tgcttcgata tctaaacgac gatcgccgat
    34201 catcatcgtg tctgttcttt ctaaatgaaa agtatcgatc aaataattta aagatgctgg
    34261 atcaggttta cgaggaaagt cctgatcgat ccctactact tcctcaatca gatgtgccaa
    34321 tcgatgttct tttaacaggc cccatgttga ttctgtcaaa cggtgggtca ggatgaaatg
    34381 acgaccgccg ttgtctttta gcatttccag tacttgtttc gtttctggaa atggacggga
    34441 aacttccgta ctttcttttt catatcggtg aaataattca ttaaactctc tggcattcaa
    34501 gtttgattca ttgatcaaat aactagtaga atactttttc attttgaagt agatttcttt
    34561 tttgtccata ctgataccaa aatcttttag tgcttgccat gcaccatcta ccattgcggg
    34621 atacgtatca aacaatgtac catcaaagtc ccatatataa tttttcatgt tccttttgtt
    34681 ccattccttt gcttcttttt tctatctctc acttttagat aaaggtgttc tttaaagtat
    34741 ttttttgtcc agaattgtca aatcatctgc taatgtatac tggattggtt gtgcatttgg
    34801 gatatcgatc gtatccattt gatcttcagg aacatcttct aaatattttg tcagtgctcg
    34861 gatgctatta ccatgaccca caactagtaa atcttgccga tcttttagtt ttggtgctag
    34921 atgatcttgc cataagggaa tgatccgatt cactgtcatt tccagacttt ctccgtatgg
    34981 gatcaaatgc ggatccaagt gatcatatct gcggtcaaat ctattttctg cggttaacgg
    35041 cggaacttcc gtatatcctc tgcgccatgt ttttacttga tcttttccgt atatttcttt
    35101 catcttatct ttgtttttac ccacaagatc cccataatgc cgttcattta gacgccatgt
    35161 ttttattata ggaagccata actgacccat ttcttctagt gcgacttgtc cggtaagaat
    35221 cgcacgtttc aaaacagagg tataaacggc atcgaattga agtccggctt caagcatttt
    35281 ttgaccggca actgctgctt gttcttttcc tttttctgtt aaggcgacat ctagccatcc
    35341 tgtccagtaa ttttcaaaat ttgcttcgct ttcgccatga cgtaataata ctaatttcat
    35401 tcttttctcc cttaaacatt cgtttcgaat tttataccac atgttttgca tgtcacacgt
    35461 attcgtcctt ttcctttagg tgcacgctgc ttttgatgac aattcgggca ctcaaaaaaa
    35521 gtataacttt ttcgatccat tttgacttgc tttttgtttt ttatctgatg atatttttct
    35581 ttgactctat caagatattt caaataacgt tgattctcat ttagtctggg gtagattttt
    35641 tttgatagga aacgaaatat catccatccg aaaaagacga tgaacaacaa ttgtccgatt
    35701 ctatagggca accaacgact tagcaaacta aagacaagcg cagcgatcaa tagaccgcga
    35761 ttcaactgat caaatttcgc atatcttccc tgcataagtt tgtgatagtt ctgctgaaat
    35821 cgtattagcc tttctaacca tgtttgcatc atattatctc ctccatcatc taatatttat
    35881 tatgacaaaa aaatccaaaa agaaaaagcc ttaattggcc ttttccattg caacttggtg
    35941 aaggacattc cgatttctgg taaagtcaat cacaatcact ttctttttat cttctctgtt
    36001 gactactcga aatgtgtccg tcaatggaag aaacagcttc ttagatggta gctgttgcat
    36061 acgtataaaa cgatggatca tcgtaatcaa ttgaggaaat tgaatagacg tgtgaagcag
    36121 acctggatat tttttcaaca gctgtgtcgt tttgatcaga ttccgttttt tactgatcac
    36181 ttttaaaaag ctttttagaa tcttttgctg acgtctaaaa atagaaaaag aattttcgtc
    36241 atctatttga taacgcattt gtttttgcag ttctgctgca gtcagatgga tcttcccatc
    36301 gtttgtatcg atcattaaac cttctggaaa cagttcaacc actgctcgat cccaagattc
    36361 aagagaaagc tcgatataat cttttactgg taattccaat tctaccttaa aaaagtgcaa
    36421 aacagccgac agcccttctg cttgatagat ttctcctaat gttagcccct ctagatggaa
    36481 ctctggtgaa aaatgcagca atgttttatt ttcctgcaat tgaacatata gaaatgtaga
    36541 tcgctcaatc agagtatctt ttaaaacaac taaaaaattc atttttctcc cccttttttc
    36601 atcattttta aaatagcata ttttgaaaag aaagtctgat gcttagagta gaatttcata
    36661 aaaatttact atttcataag tattcacgag aaaaatacaa acaaaaaagc ggaaataacg
    36721 ctcatccgcc ttcttgtttg tgtttatttc ctcttcaaac gcttgtctat gattcagact
    36781 tttctctatc gttattttca tctaaaagtt cttgcattcc gataatttct cgatattgat
    36841 gaatcttagt gataccaatg actttgtcta catcaatact aaaacaaata ccattacctg
    36901 gaatatcgat gttgatgcct tgaccaatcg catccattac tttattcgtc aaactatcgg
    36961 gtactaatgt caacacaata tctttttctg gcgaaatgtc caaacctaaa aatttccctg
    37021 aatcatgtac accagaacct cttccatgta agatcgttcc gcctgctgca cctgcttctt
    37081 ttgaaaagtc gataacatct tcgcccattc ctccgtcaac gatcgtcaca atcatgtgaa
    37141 gattcaatgc tacaagatct ttcatttgct ttcccccaat ttttgcaatc tttctttttc
    37201 gattctgtct ttttcatctt ttcgtttata aatatatcct agtactaaag tggataatat
    37261 tggtgtcaat gcaatcattg caaccacacc aaacccgtca atgatcggat ctcttccttc
    37321 aatttgtgca gcgattgcca cgctcattga taaaataaat gtcgaacaca tcgggcccgt
    37381 tgctactcca ccgttatcaa aagccattgc taaaaagatc ttgtccactt ttcgtcctaa
    37441 aataaatgct agcccgtatc ctgggatcaa gaaataataa agagagaatc cgacgagcaa
    37501 acgccacata gacaatccta ctgctgcgcc gatcccaatt gaaattacca tcagcatgat
    37561 tttcgatcgg attctcccct cgctcaattc ttcaacctgt ttgatcatta catgaatcgc
    37621 cggctcagca aaccctacta agaaccccat taaaaagcca aggggaacca gtatataatt
    37681 gtaaggcaat cccgcaatag cactgcctaa atgctgtcct accggtacat aagccatatt
    37741 gactccgtgc aggaaaagga tcaacccaat agtcgttatc aagaaacctt tcataatgtc
    37801 aaagaaacgc tttttgcgca tacgaaaact aatgatattc attaaaacaa ataggattac
    37861 gataggtgcc actgccaaaa tgacttccat ggtaacgatt tcgattccat ctatcacttg
    37921 ctgccagaaa ctcattggaa gatcaccccc aaaatcatca cggcaagaat cggccctaac
    37981 gaagcaatac cgaccattcc aaaactgtca ttttcatttt tcttttgttt gatcatacta
    38041 gtcatccctc cggcaagggc caagataaat ggaacagtta ctggaccagt agtcactccc
    38101 cccgaatcaa aagcaatcgg cataaattcg gaactggtga aaaatgaggc gatcaaaacg
    38161 cctatgtagc ctatagccat caattgataa taggatagct tgaatacgac ccgaagcagc
    38221 gcaaaggcta gaaatactcc agtgccgacg ctgacgatcc caatcagcaa cactctgcca
    38281 atctctccgt cagatatatc atatacttgt tgtcccaaaa cttgcacgga aggttcagca
    38341 atcgtgatca cgatcccgat agcaaatccc aaactgacca aaacggtcaa acttttcttt
    38401 ttgatcatat atttccctac tagatctccg acttccatca tagaataatc tgctccaaac
    38461 aaaaagagcg tcattccgat catcatgatt gccgcaccaa ccagaaatga agcgaggtct
    38521 tctccttcta acggtgcaaa aacaaacgtt aaaatgacaa tcaggacagt catcggcaat
    38581 atagcagcaa tcacttcttt aaaatttttt agcatgtatc ctcctccttt actctttaag
    38641 tttaccttaa aatgaaagag ttttcgagca caaacttaaa acgttatcat ttaatttttc
    38701 tcaacataaa ataacacacg tcccaaaagt atctttttac ttggaaaaat gactttgaag
    38761 gaacgcgctt attgtgtttt tttattttac agagccgatc attccttgaa cgaattgctt
    38821 ttgcaaaaca aaaaatacga tggcagttgg taaagtagcg atgactaatc cagccatgat
    38881 cattccatag tctggtgtat aggaagcacc catagcagat aaaacaagcg gaaccgtccg
    38941 tttatcagga gattgcaaag ctactaaggg ccataaataa ttgttccagc tactcatgaa
    39001 tgtgatgatt gcagccgcgg cataggtatt tttagccgtc ggcatataca ctctaaaaaa
    39061 aatacttagt tcttttagac cgtctaatcg agcggcttct actagatctt ttggaaaaga
    39121 tttaacattt tgtcgaaaga agaagatcaa aaaagcagtg ctaacagatg gcaggatgac
    39181 aaccaagaaa ctgttgattc ccaaaaccgt tccgttcaat tgtgaaaaca ttcgatataa
    39241 agggatcatc aatgccgcaa aaggaaccat catcgatagc agtagaatat tgaaaattag
    39301 gtcttttcgt ttactttgat aaatctcaaa tccatatcct gccattgacg aaatcagcaa
    39361 tgcaaacaca gtggtcacca aagcgatcat cgctgaattc caaagggaac gagtaaaatt
    39421 cagctcattg gaaaacaaat tttgaaaatt gatgatcaag ttactgccaa tctttatttt
    39481 tcccgatgtg atatccacgg gtgtattcgt cattcccaaa accatccaga caaaagggaa
    39541 aatggaaata aatgatagga tgcttaatgc tgtatacttt aatactgacc caattgtcga
    39601 acgaagttct aatgcttgtt ttttgacgtt ggggcgttcg attgtttctt tttctaccat
    39661 atttttcatc gactttctcc tcctactttc gtttggatta tcgacaatat cacaataaag
    39721 gaaacaataa caaaggaaac tgctgccgca tatccaaaat taggcgtgaa tttataactc
    39781 aaattataga tgtattgaga tagggtggtc gttgcatttg ctggtccccc gccagtaata
    39841 ttttgtactt catcaaaaag ctgcaatgtc ccgatcgtcg aagtaatgga agtaaacaaa
    39901 atgattggtt ttaaatttgg gatcgtcact ttgataaact gctgccattt agaagcaccg
    39961 tcaatagatg ctgcttcata aatctccgaa tcaatatttt gcatgcctga aaggaaaaaa
    40021 atcatattgt atccagtcca acgccaagtg atcgaaagaa tgatcagaac tctcgcccaa
    40081 attggatgag tcaaccagcc aatcggtgtg tcaatcatac caatgcccaa caacaagttg
    40141 tttacaaggc cagattgaga aaatagactt ttcatgatca gagaataact gaccatcgaa
    40201 gtgatacatg gaagaaagat agcggttcga aacaaccctt tatatttcaa ctttttatca
    40261 tttaaaaggt tggaaataaa aagtgctaga aacaacatga tcggtacttg tacaagtaaa
    40321 tacaggaatg tattaaaaaa agctttttta aaagttgcat ctgtcaacat ccgctgataa
    40381 ttacctaacc cgttaaatgt catattgaca gggggaccac tttgaaaaga cgtgatcaaa
    40441 gccaaaacca tcggaataaa aaccatgatt gatatcaaaa tgactggcaa gattaaaaaa
    40501 gcatgcccgt ttttgaataa atctttctgc ttcattcttt tccctccaat tctatcattg
    40561 attatctaca tgaaaagaca agaaagacaa tatctgtaag gcagacgtga tcttttttgt
    40621 cttttcgttt gtctgatgaa aaagcgaaga aattcacttt tttaccaccg atcaattagc
    40681 caattgtgct tctacttgtt tttgtgtatc tgctagtacc ttgtctgtct cttctccatt
    40741 gatgattcga tgaagagctt cagctacgac tgcttcaatt gcataggtct catgtccgta
    40801 attcacttct ggtatttcag atgtccatgt tgaaaaatcg tcaaagatct tttgaccact
    40861 gtaaaattca gaagcttctt gataagcttc ttgttcattc gcgctaagca tcgtagatac
    40921 tagtccgatt tctttcgcaa gagtgcccat cagttcttca ttcgaagcaa acgttgcctc
    40981 taggaaatct ttcgctgaat cttctccagc tactcctttg atcacatacc agccagcgcc
    41041 gccaaggtta gaagcttgcg ctttttgcag attttctgga agtgcaggta ttggtgcaat
    41101 cttccatttt ccagattggt tttctgctcc ttggatcgtt gaagaatacc aagcgccagt
    41161 tgggctagaa gctactgctc ctgattgtat cgcagttact ccagcattcc agtcagaggt
    41221 ttgttcgact agatcttctt tcaaaagagt ggcaaacaat tcaagaccat actttaaaga
    41281 tgcattgtct tggatcgtga ctgtctcccc atcttcagat gtataccatt ctcctgcctg
    41341 ttgcataatc atccttactc gaccaaggtc agaaggattg acttcagcga ttttctttcc
    41401 cgtcttttct ttgacatctc gcgctacttg aatatactcg tcccacttca gattctccat
    41461 atcctcttct gtataacctg cttctgccat cagatccgta cgatagaagt ttgctgttac
    41521 gcccgaatcg aaaggaacac cgtatatttt atctcctact ttgtttaccg caaatttata
    41581 cggtgcaaaa tgatcttcct ttacaatagc agacaaatta gcgaatgcat ctggataaga
    41641 agtcaaatag ccttgtatcc tataatcttc aatcaagacg atattgggca atccttctgt
    41701 attcccgctc gctaaagcgg tgttcagttt ctgaacgata tcatcctgag acatcgtaac
    41761 aacttctatc tctgtttctt cgttctcata aacttttttt gcttcattca ctgctttgat
    41821 attgaatgtc tcatcccacg cccagacagt gactttttct tctttgtctg ctgtactact
    41881 agcacttccg catcctgtta agaaacctga tgctgccgct aaacaaacga ctccagcaat
    41941 taactttctg atgttcattt ttatccgctc cttttccatc cattagttgt ttacgtttgc
    42001 tacattcatc ataccaaaat ttgaaaacgg tttctttatt attcagatac ttcaaaaatt
    42061 tctcctaaaa taataaaaaa atacaaatca actatgacaa aaggaataat cttagcatct
    42121 agaccaaaga tcgaaatggt gaggtttctt taaaataaaa atatccttgc attttctcct
    42181 agaaataagt atttttaaat tacaagacta ttcttaaggc gggtaaaaaa atgccaatac
    42241 gcgtaattct tatcgatgat gaacagacga ttttagatgg aatgaaacag ttgatcaatt
    42301 ggtctgagca cgggtttcaa ttaataggga ctttttccag tccgctgaaa gcgcttgatt
    42361 tttctttggt acatgcaatc gatatcgtgg tcacagatgt aatgatgcct gaattaaatg
    42421 gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct
    42481 ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc
    42541 ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcacaaa
    42601 aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga
    42661 atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac
    42721 acaccttgtt tttctgttat ataccgaaaa acggccattc agttcaaaac aaggctctaa
    42781 acaaaaacag tcgatcaccc attcattgat ggatagcgac gattcgttta ctatttatcc
    42841 tgtttctacc ttttcggaag aatccggcta tgtaatcaat actgattcca aagatacact
    42901 taggaagttt gttcaaaagt ttcgtcaaga agtttttcca ggtgattttt ttgttgtttc
    42961 tgattctttt gcaatccaag atatcaatga aatattccaa gagatcagaa attgcaccaa
    43021 aggacaacgt ttctatcata agggaaagaa ccttgcttat cagcatgaac ttcttccgtt
    43081 attaaccaac cacgagcagc aagcaagtag ttatttaaca aaaatcgttc ataaagacta
    43141 cgctttagct gtcaaagaac ttgagaattt cttcacgacg atattggaac agcctattga
    43201 tccagcttat ttgaaacatg aggcaatcaa tcgtttctat gcattactca acgctttggc
    43261 tgacgagcat gttgggaatg aagaactttc tttgctcaag atcgcccttc cttctttatt
    43321 agccgatgct gagtatttcg aagatttttc tattcttgtc aaagaaacac ttgagaaact
    43381 cacccacttt tctgcaaatg caaaaaaaga caatttcgat atcttgactt tgatctacga
    43441 gttcgtccag gaaaattatg agaaagaaat caatttacaa cttttatccg aaaaatatca
    43501 tttttcatat agctatctct cagccatctt tactgaaaaa tttggtatca acttttcaaa
    43561 atatctaaaa aaagtccgta tcagtaaagc caagctcttt ttgacggaaa cacggcttac
    43621 tttgacggaa atctgtgaga aagtcggcta taccgaactg ggttatttct ccagagtttt
    43681 taaagaagag acaggaatga caccttctca ataccgtaaa ggaacttttg taaaatgaga
    43741 aaaaaaatca aagagcatga actctttgcc aaacttctgt tcattttgtt catcggtctt
    43801 tttctgcaag ccatcgtcat ttctttgttt atttatcatc gatcaaggga tgcctatatc
    43861 cagctgttca atcaatcgaa tgatgtggta ctaaaaaaga ttcaatctga atttgaaagt
    43921 ttgaatgata caatcgagaa tacattagcc gcttttgatt ctaatccagc tgtcaaaagc
    43981 tatttttcca atgatccagc ccaacatatg gaacagcttc agcaactaag aacaatccaa
    44041 aaaatgaatg actctctttc aaaaatccat ccaatgatcg attacgatgt attgatcttt
    44101 ggagaaaatg gaagaacttt tgtcggaaac gacatgctca ctgccgtatc tgccgattct
    44161 ttttttcaat ctgcgattgc tcagagagtg aatgaacgtg ctgctgatac acaaatgctt
    44221 tttgcacacc acggattgac tcttcgcgat aagaaagcac ccagcgtctt ctttgtcaaa
    44281 aaactgaaaa atacattgaa tcacgtttac ggatatgctg ttttatcgat ctcatccaag
    44341 caactagcga atctttttca atctgtagta aatcctgaga tatcgaagat ctccttcatg
    44401 aacgaagaac agacgatcat cgcatcaaat gaagaaaata caattgggag ccgatcaatg
    44461 attttcaaaa aactaacagt aggtgaaaca acgatttcaa atggacagca gttgacccgt
    44521 cttcctctgt accgccagga ttgtacatta ctcagtcaag tcgatcttca ttctttatct
    44581 aaccaaatgg gcgtgatttt tccgattatt ttgtataata tcgtgacact tgcttttgct
    44641 ggctcattgg tatttatgta tttggatcga cacacaaaat cgatctaccg cttgattgat
    44701 tcgctaaaac ggctagagga agatggctta aaagctactg ttcctgtaca aggaagccat
    44761 gaagtccaat tgctagggaa tacgatcaat caattgctaa gtaatgctca gcatcattat
    44821 gaacagacga tccaagacga aaagaagaaa cgttcgcttg aaatccaaac tatgcaagca
    44881 cagatacagc cccattttat ttataatacg ctgactagct tgaaattttt gatttggcag
    44941 aaaaaaacag atgaagcagt tcaaggaatc gacagtttca ttactttact aagaagtacg
    45001 attgggaaaa aagaagaagt gattccagtc aaggaagaaa tcaaaagcgt acaaagttac
    45061 atcgatcttt tgagtatgcg atatggcgaa agaatctcgg ctaaaataat gattcctgat
    45121 gaactgcagt cattatctat gccgaatatg atccttcaac caattatcga aaacgcctat
    45181 cttcatgctt ttcacaataa aacgcaagga ttcatcacgg tgtacggcaa aacaaaaaat
    45241 cagcttcttt tattcgaagt gattgataac ggcgatggct tcgatactga aaataccaaa
    45301 caaaaaaatg atcatttttc aggaatcggc atcagccatg tagatgagcg gatacgccta
    45361 atgtatggcg atgcatatgg cttaacgatc caatcagtga ttgggacagg aacgactgta
    45421 acgatttgtc taccgcttct tcctaaaaat caatcagaat atcaaaaata aagaatagcc
    45481 aagcgctgat ttttttattt taagttaaat aaatcaatca gtattcttga ctttcatcta
    45541 cgcgtctctt ctactgtcag cactggataa cagcccaata tttgactttc tcctccgagc
    45601 atcttgattt cttggagcgc attctctact aatgtcattg gacggtccaa caacaaatcg
    45661 atcacgaaaa aatattctcc caagctagtt ttcaatggtc gggattcaat cttacttaag
    45721 ttgatttccc gccagccaaa agctgccagc attttatgaa gcatccctgg ccgattagca
    45781 ggtagtgtca agatgacact catttttact ggagctgact gctggctggt catctttcgg
    45841 tcaccgacga tccaaaaacg tgtctggttc aactcattat cttggatatt ctcagctaag
    45901 atctccaatc caacatgctg tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc
    45961 ggatgttctg ctacatacat tgctgcagca gttgttgatt ctgttgctac taaaggaaca
    46021 ttaggataat gcgtttctaa aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg
    46081 atttttgtaa tctttgttgc tggattgcct aaaagctgct gcttgatagg aagaacgatt
    46141 tcacttttaa cttcgacttc tggatgtttg gaaagaagat cgattgtgtg atgaacagct
    46201 ccttccaatg aattctctac aggaacgaca gccagatcga cttgtttgcg aaacaaagcg
    46261 cgcaaacaaa cagggatgga agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa
    46321 gccgcctgat aagtgaatga actttctggc cctagatagc tgacgttcat atgcttttga
    46381 tcctttctat aatggtatcg atgatctttt ctggtgtttg attggtcgtg ttgattcgat
    46441 gtgtcgcact ttcttcatac caagaaagtc gttgtgccaa taatgctttt atttcttctc
    46501 ttgtttttcc aagagctaaa ggtcgaatag acgtttcatc ttgttggata cgatcaacta
    46561 gacagtccgc ttctgcctct aagtaaagaa caatcggctg tgtttttaaa aaacgccgat
    46621 tttctgcact ttggacaatc cctccaccag tcgcgataat tttgtcttca tttgacagct
    46681 ttcttaaaat atctgactcc acttttcgaa agcttttttc accgtaatag tgaaaatatt
    46741 cggcaatcga caattggata tgttcctcaa tctttgtatc taaatctaca tactcttttt
    46801 gtaaacattg agctaaccct ttaccgattg ttgtttttcc agcgcccatg aaaccaatca
    46861 gcaaaatgct agccatgagc ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat
    46921 gccggataag agattgaaac agcttcagct ccttctagtt cgcaaggtcc atcagctatc
    46981 aaagcagcta cttgcagcat cataccgatt cgatgatcgc ctctgctgga cacttttgcg
    47041 ccatgcagag gtgtcggccc atggatgatc agaccgtctg ctgtcgcttt tatctttgct
    47101 cccattttct gtaattcttc tgccaccgca tcaattctgt tggtttcttt tacttttaat
    47161 tcttctgcgt ctttgatgat cgtgattcct tctgcttgag tagccactaa agctagaatg
    47221 ggcaattcat caatcagacg cggaatgatt tctccaccaa tcgttgcttt cttcaaatga
    47281 ctcgtcttga cacttaaatc agccgattgg ttctgttcat tgtgattcgt ctgggtaatc
    47341 gctgcgccca tctcctccaa cacatctaag ataccagttc ttgttggatt gaccccgaca
    47401 ttttttaaca acagttggct ttccggaaca agaagtcctg ctgctagaaa gaaagcagct
    47461 gatgaaatat cacctggaac agttatctgt tgaccagtta atttttgcgg tccggtcact
    47521 atgatcgttt tatcttccac tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta
    47581 tggttacggg aacgttcttt ttcaacgatt acggatgtgc cttctgcttg taatgccgca
    47641 aacagcaaag cagatttgac ttgcgcactg gctacaggca tattgtatcg gataggtgat
    47701 aaagagcggg ttcctgtcac agtgattggt agatactggt cattttcttc tccttgcaag
    47761 tcagcgccca tctcgcgtaa aggttccatc actcgcccca ttggacgttt agacaacgaa
    47821 gcatccccga acagagtagt cgtaaaaggc tgtccagcca aaatacccat caataaacgt
    47881 atggtcgtac cggagtttcc catgtcaatt gattcggttg gttggacaaa tgagtggcta
    47941 ccgcgaccat gaatcttgat cacctcttcc tcttcttcga tctcgacacc taattgttgg
    48001 aaggcatgta aagtactcag acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt
    48061 ccttcagcaa ttgctccaaa catgatgctg cgatgggaaa ttgatttatc tgctggtatc
    48121 ctaacagtcc ctcttaatcc atgtatttgt tgtaataatt gcatttatcc agcctcctat
    48181 ttgatccagc aatgataact ggtattagac ataatgattc tttttgcttg taacaaatcc
    48241 tgttggtttt taaaactgat ttgcagtatt cctaaaatat cttccctggt ctcttggatc
    48301 ttcaaattaa tcaaagaaac tcccgctttt ccgatcagtc cagttacttc tgcaataacg
    48361 ccaggttcat ctggcacatc gataaataaa tcaaaaaagg caggaatcgc gccttgctta
    48421 tggacaggca actggtctcg actttctttt gcccgattga aaaaagaaaa gatttccgat
    48481 tggttctcag tcatgatcca ttctgctatt tgtgtcattt cttgttgcca atctgccaat
    48541 cgttccaata atatctgctt attgctcaac aaaatatctg tccacatgac aggatctgag
    48601 gaagcgatcc tagtgatatc tcgaaagcct cctgctgcca gttgtttggc tcgcggatac
    48661 tcttgattga attgttctgc ctgattgact aagccagctg ccagaatatg tggaaaatga
    48721 cttagcattc ctgttatacg gtcatgttct cttgctgtaa gagttacata ttttgctttg
    48781 gttccgcaaa atagttcttg cagtttttca acatcttttt tcgtcttttc caaaggtgta
    48841 aagatgtaat aggcattttc aaatagttta gcatctgctg cctgtatacc tgatttatga
    48901 gaaccagcca ttggatgtcc accgacaaac cgaagatttt tctgctcagc ggatgtcacg
    48961 acttgctgct tcgtactgct gacatccgta atgatgacgt gatcagacaa gttgattctt
    49021 tcaaggacat caagataagc taaggttgta cggacgggaa cggctaataa gataacgtct
    49081 gcttgttctg cacctgtttc aaaatcactt gcaacgtaat caacgatctg ctgttctact
    49141 gcttgctcac gggtttttga ctgactatcc caaccaacta ctatcagttc tggattggtc
    49201 tgacgaatac aaagtgccat agaagcacca atcagtccaa gccccacgat aaaaacatgt
    49261 tgtgccatct tatttgcccc ctttttagta atgtttcaag tagtcacggt actccttcac
    49321 tgcagctttc aactcaacaa gcgaatcact tgggaatttt gacaatagct ctgtcgctac
    49381 cacagtggct acaacatttt ccgcaacgac gctagctgca ggaactgctg tactatccga
    49441 acgttctaca cttgctttgt aaggttcttt tgtatcgata tcaacacttt gaagtggctt
    49501 gtacaatgta ggaatgggtt tcatgacccc gcgaatgaca agtggttcgc cattggtcat
    49561 tccgccttcg aagccgccta gattattggt acgtcggaaa tagcctcttt cttcttccca
    49621 taggatctca tccatcactt ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa
    49681 ttctacgcct ttaaaggcat tgatactaac cacagcttgt gcgattttgg catctagctt
    49741 cgtgtcccat tgtacgtagc ttcctaatcc agctggaacg cctccaacga gcacttctac
    49801 aattcctccg atcgtatctc catttttctt tgtctgatcg atcaaatctt ttattctttg
    49861 ctcaactgct ggatcaacaa ctcgtacttc tgacgcttca gaaatttctt tgatttcttg
    49921 aatcgagata tttccaggta tttctgcttg gattccacca agacaaatca cgtgtcctgc
    49981 tacgatgatg tcaagttctt ccagaatttt cttggcaatt gcgccgattg ctacacgcat
    50041 ggtcgtctct cttgcagagg accgttcaag cacattccga agatctcgat gatggtattt
    50101 cataccgcct accaaatccg cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg
    50161 gtctttttca ggcacttctg ctacagacat gacagttgtc caatttttcc aatctttatt
    50221 ttctaccact aaagtgacag gtgaaccaat tgttttgccg tgacggatgc cagacgtgat
    50281 ccgaacacga tctttttcga tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg
    50341 agcgagttcg tgattgattt cttcttctga cagaggtaat cctgctggta gtccttcaat
    50401 aatggctgtt aattctggtc catgggattc tcccgcagtc atataacgca tcttacagtc
    50461 ctcctaagta atctttgaat gtctctgtgg gaacaggaac gatttttgct tgcccaatct
    50521 tttctagcaa gatgatattc aattggcttc ctcttgcttt cttatcatgg gttatcgcgt
    50581 ggaataattc atctggatga cttagatcgt acgtaatcgg cagatggaat ttttgaagta
    50641 tcctttctaa agcagctgtc gatccagttg gagacaagcc gattttttct gcatgagaag
    50701 tgatggctac cattccaata gctactcctt ctccatgact gactacccca tatcctgctg
    50761 tattttcaat tgcatggcca attgtatgac caaaatttaa aataagtcga tttccttggt
    50821 caaagacatc ttcttccact acttttcgtt tgatttccaa cgtagcagta atgattggaa
    50881 cagcatgttc cagaagatct ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg
    50941 catctgctat agctgctgat ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg
    51001 gcaaagtttt tagaacctca ggatcaatca atacaccagc tggctggcta aatgttccga
    51061 caaggttttt cgctttgttt gtattgactg ctgtcttccc gccaatacta gaatctactt
    51121 gtgcaagtag cgtagtcgga atttgaacga aatggatccc acgcatgaac gtcgaggcca
    51181 cgaatgctgc tagatcaccc accattcctc caccaagtgc aagtatccca tcgctgcggg
    51241 taaaatcatt atctgctaaa aaatcgtata actcagctgc ttgttctaaa gatttactgg
    51301 tctcacccgc cggtacgatt gaaaggactg gttcaaaatt agcttgtctc aattggaaca
    51361 tgattttttc tgcatatagt ggagcaacgt ttgtatcagt gatcacagct actttttgag
    51421 gtctccaaat cgtttgaagc catgatccag cctgttctag tgcgcctttt tcaatatgaa
    51481 tatcgtattt ttggttatca acatgtacag taagcattcg tttcacttct ttcttttaaa
    51541 aagctggaat taatcgagtg tctgaatctt ttccattgct tctttcatga tcgcaacttc
    51601 tttcatcatg cgtgagtaag tcttttcatt caacgattgg ggaccgtctg accaagcatt
    51661 tgcaggatca ggatggatct ctacgatcat gccatctgct ccgcttgcca cacccgcacg
    51721 agccattggt ggtacaagtt cccaaattcc tgtaccatga cttggatcaa caatcacagg
    51781 gaaatgagtt aattttttca tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc
    51841 tgtttcgtaa gtccgaatcc cacgttcgat aaaaatgaca ggagatttgt cttcgactgc
    51901 aatgtattct gcagcattaa gccattcatt gatcgtaccg gaaattccac gtttcaatcc
    51961 aacaggttta cctgttttac cgacagcaga tagcagttta aaattttgca tattacgtgc
    52021 gccaatctgt aggatatcgc tgtactcagc caccatatcg atatgccctt catccatcac
    52081 ttcagtaatc actttcattc caaattcatc agccgcttga cggatgtatt tcaatccttc
    52141 ttcttctaaa ccttggaaag catatggaga agtacgtggt ttgaaggcac cacctcgcag
    52201 aatttttgcg cccccggctt tggccattcg cgcacattct ctgatttggt ctaatccttc
    52261 gatagaacaa ggaccagcca tcgtcacgaa actgccgtca ccaattttta ctccgtctac
    52321 atccacgact gtattgccag gatggaattc tcgagaagtc aatttgtatg tgtttaaaat
    52381 acgcacagca ttttctactc cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt
    52441 atcacctttt agtccaatca ctactcgttc tgttccttga tcgattgaga ctttgaatcc
    52501 ttctttttcg atatgatcga cgactttttt tatttgttct tccgttgctt cttttttcat
    52561 aattactatc attgtttttc ccgctttctt ttgtttactt atctatcttg gctgattttc
    52621 tctacttttt caaggtggtt ttacattaat tcctactaaa tgaccgtgtc ctgttgttct
    52681 cattttcgct ccttctttcc tacttatgac ccagataatc aacaaaaaaa gatgtccctg
    52741 taggaaattg cctctacagt gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat
    52801 aaaatagcca ctatagatga atttctctac gtggctgtaa gtcgtgttta aaaatcttta
    52861 gccatcatag atacaaacct ggtttgtttg tacccacgaa gtcatgaata caaacctatc
    52921 taaaatagct aaagtaatac gtattcagtt ttgtaagttt tctagatttc ataaaaaatc
    52981 gctccaaact gatgtaagaa ttgtacctag tatagacata aaaaaatcga ctgtcaatca
    53041 tttttatgaa acttttttta attttctgat agttaacttt gtttttcaga aaaataacaa
    53101 ttggttttat tgaagaaaat agtcatgaac aaaagtatct gagaactttt ttactctctt
    53161 gttcatgaca tatagaataa agcagctttg tcttgtaaac tccgtttttc aaataataat
    53221 ctaaagattg ctactgaata ataaagccga tttcatggtc ctcttcgctg ctgattcggt
    53281 aaggtgactc aacatccgct ccccagctat tgataccccc gactcctctt acttttgcaa
    53341 aaatcgtaag aaccgttcgt ctttctggcg gtagttcttc tatatgtgtt gcctgttcca
    53401 gttcttctgc tttgaatggt aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc
    53461 gcaacgaaaa atcttcttga tctttatccg tattatccaa cgtttgattt cttgttagtt
    53521 ctacccattt cgtttccata tgcatgccgc attcttgggg aacaaggtat ggggaaacgg
    53581 ggagtcctgc cactttataa ataccttcga caccaccagc catccgatcc ggatatgttt
    53641 cccctgatag gccttcatac tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac
    53701 ccaatgcggg taattccggt aatccttttt gtccataata atgcatattg acaagcattt
    53761 taccctctcc agcgatttgg taggaaattg tcacttgggt ttttggtact gtgttgattt
    53821 ccagtgtaaa aatgatttca accgtttcag cgtattcatt acttgaatat tgattattta
    53881 ctggagcaat cggcaaatcg atctttttat tgtccaccag tacttctatt tctttgactt
    53941 ttggatacaa tccggctcct aaccacatac cggatttaaa tccaaatccg ctacctctgt
    54001 cattatcggt cgttgctcgc caaaaaactg gaagtgtttc ccggtatagc cattcttttc
    54061 cgtttttatt gagcgattcc agtccgcctc ttgtataaga aaaaatataa tgcgtattcc
    54121 ccgatttgac acctaatgta cagtcgccga agacgattgc aactttttcc gtatccatat
    54181 gacttttatt tactgtattt ttcataataa tatcttacct cctgcatggc gggtttttct
    54241 tgtccatctg caaaaacgat cccattccct gagaattcat agtcacttgg tttatcgtca
    54301 aagtcaccgc catagcgcat cacttctctg cctgtgattg gatcagtcac ttggatcgcc
    54361 tgatctttaa aatcccatat atagcctcct tggtacattt cataacgatc aagcaacgtc
    54421 atgtaagaat ccattccacc taacgaattc cccatgtcat gcatatattc acataagatg
    54481 aacggttttt ccgctttatt ttcaaggtag tcaatgatct cttctggtgg cgcatacatg
    54541 cagcttttta catctgaaat cgaatcttcg aatgctggat ttggaaaatt cccttcataa
    54601 tggacaagtc gatcggggtc tacttttttg aagtaatcgt tcatttgtcg aatgttttct
    54661 tccgcataag attcatttcc aagagaccaa aaaagaatac ttggatggtt ttttaatgtt
    54721 tcgaaattcg tttttgctcg atcaagaaca gccgcctgcc actgaggtac tgcacctggt
    54781 acgttgtaag aaggctcaaa tgcgcccatt ttttgccatg agccatggga ttcgaggttc
    54841 gtttccgcca tcacatggat tcctgcttgg tcgcataaat aataccaagt gatctggttg
    54901 gggtaatgag atgtccgtac gccgttgatc tttgcatttt ttatgatagc caaatctttt
    54961 tccatatctt ctatagtgat ggcgcgtcct tttttcggat gccactcatg tcgatttacc
    55021 ccgttgataa tcatccgttt tccattgaag tagatgattt tatctttgat ttcgatttta
    55081 cgaaaaccga aggaatagtc aatatactcc gttactcttt tttcttcgtc tatcgtctcc
    55141 aattcaagct tatataaatt gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca
    55201 tcgatgcgaa tcgtcatttg ctcttttaac ggatattgct gttgatagat ttccttccca
    55261 tcaggatcta gtatcgctac attcagctga ctgtttttgg ctacttgctc aaaagacaaa
    55321 tctacttcaa gtttgccatt ttgacatttg tcatctaagc gaggtttgat ccaaagatct
    55381 tctaaatggt tttgtcctcg agcaactaag aagacatctc tgaagatccc aaaaaatcgg
    55441 aaaaagtcct gatcttccaa gaatgcagct gtacttcttt tgtaaacctc cacagctaga
    55501 atattttctt cttccaaata aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg
    55561 gcatacccga taaaatgacc attcaaccaa ataaacattg cttgttctac accagcaaaa
    55621 tgaatcgaga tttgttttcc ctttagacct tcttctaatc gaaaagtcgt caaatacgaa
    55681 ccaaccggat tgtctttagc agctgaaaag atcccttccg cctctttctc agggcttgtc
    55741 gtgaaagggg gacggcgaaa tacatgtccc tcccacggat acatcgtatt gatgtaatgg
    55801 atcttgtcat aatttgctaa ttcaatatga ctaggaacag tgatttcatc aaaagaacag
    55861 gcatcaaaat cttttttata aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa
    55921 aaacgccatg atccatttaa agactgttta agggaggatg tggtttctac ttcttccctt
    55981 gttgcataac aaacatgatc gctatgtgca tttgattggt tcactctaaa aacttgtgga
    56041 tcatctaacc attttatatt tgctttcata cttttttcct tctctctcat aactttttga
    56101 aatgaagcag ttctgtttgg aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca
    56161 tcaattcggc ccccgaaaat atccgcttcg aggtctgttc ctgataaagt gcctcatcat
    56221 ctaaccccat cagatatact tcatggaacg ctggttgagc tgaagctagt atcctcccta
    56281 aaaacacaat agcttcatct tgtttaggag acacaaacat ccatgcggca atattttctt
    56341 caaaaggact cgccaaacga taataatggc catactggat caccggacga atcgtttgat
    56401 aggatataat ttggtctttt acttgctttt tttcttcagg agacagcttt gtcaaatcca
    56461 gttcataacc aaaaacagcg ctcatagctg tttctcctct tgttttcaac gaagtagatc
    56521 tgtcggtctg atgatttggt acagctgata catgagctgt gatggacgaa atcggataag
    56581 caaggcttgt tccatattgg attttcatac gctccactgc atccgtgtta tcacttgtcc
    56641 atgactgcgg catataataa ataaacccgg catcaaaccg tccacctcca ccagaacagc
    56701 cttcccataa gatttccgga taatcagttg tcaaatgttc aagtagttca taaagcccga
    56761 gcatataacg atgcgctact tctccttgtc tctgtgggtc aagggtaatc gaatagacat
    56821 ctgaaagcga acggttcata tcccatttga tgtagtcaag aggaatcgta tctaaaatct
    56881 ttctcatttg tttctcaatc gtctgccgaa catctaacct tgtaaaatcc agtacatgtt
    56941 gtgatctgga agcagaaggc attctacctg gctcctgcat aagaaaatca ggatgtgttc
    57001 gatacagttc agaatcaacc gagatcattt caggttcaaa ccataaacca aatttcaatc
    57061 ctttgctatg agcataatct gctatttcac gtaatccatt tgtcaacttg ccttcatact
    57121 cgaaccagtc tcctaatgaa gaattgtccg catctctttt tccaaaccag ccatcatcta
    57181 atacgaacat ttcgatgcct agctcctttg cttcatcaat gatcgccttg attttttcac
    57241 tattgaagtc aaagtaagtg gcttcccagt tattgaccag aatcggccgt tcagcaaatt
    57301 gatgacgacc tcttgcgatt ctttcacgta gcaactcatg gtggatctga ctcattttgt
    57361 tcaatccctg acttgagtaa gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat
    57421 tcaattccca cgaaaaacga tggctgttga tcccaccgac gactctcaat tgattgatct
    57481 gatctttttc aagttcgaaa gaatggttgc cggaataaac aaattgaagt ccgatcgctt
    57541 ctccagtatt ttctgtcgta tgatgatgaa ctaaagcaat aaagttattc atatggtgac
    57601 tgctcgttcc tcgacggctt tcaaatactt ttgttccaga taaaacagat tgccgtgaaa
    57661 tctgtctttc attgacatgt gctcctggca aagcaatgac ttcatcaaat cgcctagtat
    57721 gggcaaagtc tagctggaaa ctagccgctt tttctagttt gattgtttct ccggtttcat
    57781 ttcttatctt tacagatcgt gtgatcactg ctctatcagt aaatatggta tagaacaaat
    57841 cgaaataaat cgctgtttct ctatccttaa gtgaaatcac caacgtttgt gcctctgact
    57901 tgtccatcac ataagtttga ggcaagcctt cgatctctgg cttcccatct gtaatataat
    57961 ggctgtcata tctcagatcc agtaagtctg atccattctt gcgctgaatg atcgatgcgg
    58021 gcgttcgaaa gtccatgctg ccatgagaag agtattcttg cggtagtgta tctttcgaca
    58081 gagaccggtc aggatttaag ggaacatttc ctgaaaatcc acgatctctt cggggataat
    58141 ttttattgtc tttgtactgc ttgactggtt tgccaaaata aatatgcgcc aatacattgc
    58201 tttcttctat ctttatcaga taagaaagaa atgtatttcg taaatggaag actcggctct
    58261 tttcatcaaa taagatgaaa tttttatctg tttttctatg cgtaatatcc atcatttcac
    58321 tcccaacaaa acggaaaccg tttacttttt tttataattc ctgtatactg tttactaaac
    58381 aattttgtca ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag
    58441 agatgtcgca cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga
    58501 tcagacgatc catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt
    58561 ggattatgac atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat
    58621 ctctacgatc ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg
    58681 gatggaagaa gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga
    58741 taatcctaaa caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt
    58801 tacgaaagaa tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa
    58861 ccctgacatc cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac
    58921 cgtactggaa ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa
    58981 agtcgatatg gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc
    59041 ctatcagcat tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg
    59101 ctggacagaa aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct
    59161 tcctactgcc attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa
    59221 acaaaaaaat atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac
    59281 aatcgcaaac ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca
    59341 agcagctgtt cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt
    59401 gacttatcct gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg
    59461 aaagagaggt tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca
    59521 caacctctta cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt
    59581 cttttagcgt gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa
    59641 acaaggattg cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct
    59701 cctggaatga gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat
    59761 ggtcagccac actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca
    59821 acgaccacgc aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg
    59881 ctgttagctt caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg
    59941 gaagtagctg tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat
    60001 attgcaatgc gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta
    60061 gcaatttgtc gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa
    60121 cttgattagg gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt
    60181 tggcgtctga aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga
    60241 atcctccacc atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt
    60301 agatgtcata agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca
    60361 cgtttttttc tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca
    60421 tatggttgat caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa
    60481 acgcgatccc aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca
    60541 taaggctccc tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat
    60601 caagctctcc tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa
    60661 aagaaatttt ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc
    60721 acattgagga cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg
    60781 gtcaatcact tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga
    60841 caactaattt atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat
    60901 cgtattctaa ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg
    60961 tcatcccccc tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta
    61021 gtgttgaatc atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat
    61081 tgaaataacg aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca
    61141 caactcaaca gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa
    61201 gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga
    61261 ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc
    61321 ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta
    61381 ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac
    61441 aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa
    61501 agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc
    61561 aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat
    61621 ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt
    61681 ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc
    61741 catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga
    61801 ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta
    61861 cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt
    61921 ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc
    61981 taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa
    62041 ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac
    62101 taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg
    62161 tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga tatctctgac
    62221 tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag
    62281 atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg
    62341 gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg
    62401 aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac
    62461 aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt
    62521 gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc
    62581 aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc
    62641 gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac
    62701 gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc
    62761 ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat tacacaacta
    62821 aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt
    62881 gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct
    62941 tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca
    63001 tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc
    63061 atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca
    63121 tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca
    63181 tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc
    63241 ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg
    63301 aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc
    63361 ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct
    63421 ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc
    63481 aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg
    63541 atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct
    63601 ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt
    63661 ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca
    63721 ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct
    63781 gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc
    63841 tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt
    63901 acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta
    63961 ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag
    64021 ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa
    64081 atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt
    64141 tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg
    64201 atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt
    64261 gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc
    64321 atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa
    64381 tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca
    64441 cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga
    64501 atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca
    64561 ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt
    64621 gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa
    64681 gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg
    64741 aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct
    64801 tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa
    64861 gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg
    64921 ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca
    64981 tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt
    65041 tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga
    65101 ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt
    65161 aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg
    65221 cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct
    65281 ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg
    65341 actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt
    65401 ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac
    65461 cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg
    65521 gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat
    65581 cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt
    65641 cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt
    65701 ttttgaagta agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc
    65761 gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt
    65821 tcacattttc agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg
    65881 cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga
    65941 cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt
    66001 ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt
    66061 tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt
    66121 ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg
    66181 tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac
    66241 ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc
    66301 ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac
    66361 caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat
    66421 aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga
    66481 tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt
    66541 aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac
    66601 caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg
    66661 cctgttccaa tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt
    66721 ctttgattcg ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa
    66781 gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc
    66841 cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt
    66901 caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt
    66961 ttcaaccaca ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac
    67021 catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc
    67081 aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct
    67141 tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa
    67201 attgtttatt gaaatttgtc tgaaaaaact catttttcat tcttctactg agcgactttt
    67261 agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt
    67321 ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct
    67381 accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg
    67441 tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa
    67501 ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat
    67561 caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc
    67621 gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc
    67681 gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa
    67741 aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc
    67801 tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc
    67861 agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc
    67921 atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg
    67981 gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc
    68041 acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa
    68101 cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt
    68161 caagcggata tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt
    68221 cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc
    68281 gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc
    68341 tgctgcccat ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt
    68401 cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc
    68461 ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg
    68521 gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc
    68581 gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc
    68641 taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc
    68701 tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct
    68761 tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt
    68821 ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc
    68881 atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac
    68941 tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa
    69001 aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac
    69061 ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc
    69121 gattgctact aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc
    69181 ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt
    69241 agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt
    69301 caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta
    69361 gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt
    69421 gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg
    69481 gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg
    69541 tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta
    69601 caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg
    69661 ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct
    69721 ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa
    69781 tcaggaatac aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt
    69841 gggaagcatc ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga
    69901 ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt
    69961 cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta
    70021 atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca
    70081 attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg
    70141 cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt
    70201 caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc
    70261 cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta
    70321 ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc
    70381 ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt
    70441 gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct
    70501 cttcttgcat ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt
    70561 aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat
    70621 tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt
    70681 ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat
    70741 ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa
    70801 atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat
    70861 ctacatgaat ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa
    70921 ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct
    70981 ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt
    71041 ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac
    71101 ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg
    71161 tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat
    71221 ctcttcttaa aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc
    71281 cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag
    71341 taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt
    71401 gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag
    71461 tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg
    71521 acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca
    71581 ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt
    71641 tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca
    71701 agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga
    71761 caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa
    71821 gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca
    71881 tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg
    71941 ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt
    72001 tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag
    72061 aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga
    72121 gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag
    72181 cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt
    72241 ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt
    72301 actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat
    72361 attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga
    72421 atgtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg
    72481 ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg
    72541 gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg
    72601 gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg
    72661 ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta
    72721 tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg
    72781 gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg
    72841 ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca
    72901 atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata
    72961 ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga
    73021 gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct
    73081 ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag
    73141 atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg
    73201 ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta
    73261 caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg
    73321 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
    73381 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
    73441 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
    73501 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
    73561 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
    73621 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
    73681 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
    73741 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
    73801 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
    73861 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
    73921 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
    73981 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
    74041 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
    74101 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
    74161 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
    74221 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
    74281 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
    74341 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
    74401 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
    74461 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
    74521 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
    74581 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
    74641 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
    74701 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
    74761 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
    74821 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
    74881 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
    74941 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
    75001 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
    75061 taactgcact gtttttcgcg tgaggcatgg cgattccatc accaagacct gtagaagttt
    75121 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
    75181 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
    75241 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
    75301 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
    75361 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
    75421 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
    75481 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
    75541 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
    75601 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
    75661 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
    75721 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
    75781 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
    75841 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
    75901 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttc
    75961 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
    76021 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
    76081 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
    76141 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
    76201 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
    76261 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
    76321 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
    76381 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
    76441 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
    76501 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
    76561 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
    76621 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
    76681 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
    76741 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
    76801 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
    76861 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
    76921 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
    76981 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
    77041 attataaatc agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt
    77101 ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa
    77161 acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac
    77221 atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg
    77281 gataataatg atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc
    77341 tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg
    77401 cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg
    77461 tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga
    77521 ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt catctgttac
    77581 ctctacttct agatattccc taatataata acaataagct tcgattccat tatccaaagc
    77641 aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg
    77701 aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg
    77761 gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacgg gttgcatttg
    77821 tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg
    77881 cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct
    77941 aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag
    78001 ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag
    78061 aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt cccaacacga
    78121 agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac
    78181 tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa
    78241 caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat
    78301 ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg
    78361 cttttctttg aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa
    78421 gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga
    78481 tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa
    78541 agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc
    78601 ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc
    78661 gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa
    78721 gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat agactaagta
    78781 aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat
    78841 aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc
    78901 gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa
    78961 gttaagctc
//
LOCUS       DACCDF010000002        78507 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCDF010000002 DACCDF010000000
VERSION     DACCDF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.002"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(606..962)
                     /locus_tag="IVK51_000072"
     CDS             complement(606..962)
                     /locus_tag="IVK51_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP6429701.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /locus_tag="IVK51_000073"
     CDS             complement(976..1386)
                     /locus_tag="IVK51_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6429702.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1732..3918)
                     /locus_tag="IVK51_000074"
     CDS             complement(1732..3918)
                     /locus_tag="IVK51_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6429703.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNISVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4136..4819
                     /locus_tag="IVK51_000075"
     CDS             4136..4819
                     /locus_tag="IVK51_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP6429704.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4900..5619)
                     /locus_tag="IVK51_000076"
     CDS             complement(4900..5619)
                     /locus_tag="IVK51_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6429705.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5931..6137)
                     /locus_tag="IVK51_000077"
     CDS             complement(5931..6137)
                     /locus_tag="IVK51_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429706.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6382..6984
                     /locus_tag="IVK51_000078"
     CDS             6382..6984
                     /locus_tag="IVK51_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP6429707.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7125..8406
                     /locus_tag="IVK51_000079"
                     /pseudo
     CDS             7125..8406
                     /locus_tag="IVK51_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
     gene            8419..9147
                     /locus_tag="IVK51_000080"
     CDS             8419..9147
                     /locus_tag="IVK51_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6429708.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9216..10739)
                     /locus_tag="IVK51_000081"
     CDS             complement(9216..10739)
                     /locus_tag="IVK51_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6429709.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10991..12172
                     /locus_tag="IVK51_000082"
     CDS             10991..12172
                     /locus_tag="IVK51_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6429710.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IVK51_000083"
     CDS             complement(12232..13860)
                     /gene="treC"
                     /locus_tag="IVK51_000083"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP6429711.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14311..17022)
                     /locus_tag="IVK51_000084"
     CDS             complement(14311..17022)
                     /locus_tag="IVK51_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6429712.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGTIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(17032..17733)
                     /locus_tag="IVK51_000085"
     CDS             complement(17032..17733)
                     /locus_tag="IVK51_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429713.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17919..18518
                     /locus_tag="IVK51_000086"
     CDS             17919..18518
                     /locus_tag="IVK51_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6429714.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18599..19612
                     /locus_tag="IVK51_000087"
     CDS             18599..19612
                     /locus_tag="IVK51_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6429715.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19720..20637
                     /gene="rbsK"
                     /locus_tag="IVK51_000088"
     CDS             19720..20637
                     /gene="rbsK"
                     /locus_tag="IVK51_000088"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP6429716.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20665..21060
                     /gene="rbsD"
                     /locus_tag="IVK51_000089"
     CDS             20665..21060
                     /gene="rbsD"
                     /locus_tag="IVK51_000089"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP6429717.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            21060..22433
                     /locus_tag="IVK51_000090"
     CDS             21060..22433
                     /locus_tag="IVK51_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429718.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22790..24934
                     /locus_tag="IVK51_000091"
     CDS             22790..24934
                     /locus_tag="IVK51_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6429719.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24986..26027)
                     /locus_tag="IVK51_000092"
                     /pseudo
     CDS             complement(24986..26027)
                     /locus_tag="IVK51_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
     gene            complement(26304..28106)
                     /locus_tag="IVK51_000093"
     CDS             complement(26304..28106)
                     /locus_tag="IVK51_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429720.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28120..28872)
                     /locus_tag="IVK51_000094"
     CDS             complement(28120..28872)
                     /locus_tag="IVK51_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429721.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28881..29243)
                     /locus_tag="IVK51_000095"
     CDS             complement(28881..29243)
                     /locus_tag="IVK51_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6429722.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29419..30291)
                     /locus_tag="IVK51_000096"
     CDS             complement(29419..30291)
                     /locus_tag="IVK51_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP6429723.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30342..30830)
                     /locus_tag="IVK51_000097"
     CDS             complement(30342..30830)
                     /locus_tag="IVK51_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP6429724.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30968..31759)
                     /locus_tag="IVK51_000098"
     CDS             complement(30968..31759)
                     /locus_tag="IVK51_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP6429725.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GKSYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31814..32422)
                     /locus_tag="IVK51_000099"
     CDS             complement(31814..32422)
                     /locus_tag="IVK51_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP6429726.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32415..32732)
                     /locus_tag="IVK51_000100"
     CDS             complement(32415..32732)
                     /locus_tag="IVK51_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6429727.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGDALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32888..33616)
                     /locus_tag="IVK51_000101"
     CDS             complement(32888..33616)
                     /locus_tag="IVK51_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP6429728.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33775..34656
                     /locus_tag="IVK51_000102"
     CDS             33775..34656
                     /locus_tag="IVK51_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6429729.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERANRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34893..35936)
                     /locus_tag="IVK51_000103"
     CDS             complement(34893..35936)
                     /locus_tag="IVK51_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6429730.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35939..36607)
                     /locus_tag="IVK51_000104"
     CDS             complement(35939..36607)
                     /locus_tag="IVK51_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6429731.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36749..39085
                     /locus_tag="IVK51_000105"
     CDS             36749..39085
                     /locus_tag="IVK51_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6429732.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39200..39511)
                     /locus_tag="IVK51_000106"
     CDS             complement(39200..39511)
                     /locus_tag="IVK51_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429733.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39692..41167
                     /locus_tag="IVK51_000107"
     CDS             39692..41167
                     /locus_tag="IVK51_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP6429734.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPTMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41214..41798
                     /locus_tag="IVK51_000108"
     CDS             41214..41798
                     /locus_tag="IVK51_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP6429735.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41800..42219
                     /locus_tag="IVK51_000109"
     CDS             41800..42219
                     /locus_tag="IVK51_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP6429736.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42500..42784)
                     /locus_tag="IVK51_000110"
     CDS             complement(42500..42784)
                     /locus_tag="IVK51_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP6429737.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43016..43726)
                     /locus_tag="IVK51_000111"
     CDS             complement(43016..43726)
                     /locus_tag="IVK51_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP6429738.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43723..45195)
                     /locus_tag="IVK51_000112"
     CDS             complement(43723..45195)
                     /locus_tag="IVK51_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6429739.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45333..45806
                     /locus_tag="IVK51_000113"
     CDS             45333..45806
                     /locus_tag="IVK51_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6429740.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45959..46510)
                     /locus_tag="IVK51_000114"
     CDS             complement(45959..46510)
                     /locus_tag="IVK51_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6429741.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46615..47145
                     /locus_tag="IVK51_000115"
     CDS             46615..47145
                     /locus_tag="IVK51_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP6429742.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47628..48071)
                     /locus_tag="IVK51_000116"
     CDS             complement(47628..48071)
                     /locus_tag="IVK51_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429743.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48087..49247)
                     /locus_tag="IVK51_000117"
     CDS             complement(48087..49247)
                     /locus_tag="IVK51_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6429744.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49754..51454
                     /locus_tag="IVK51_000118"
     CDS             49754..51454
                     /locus_tag="IVK51_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429745.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51556..52389)
                     /locus_tag="IVK51_000119"
     CDS             complement(51556..52389)
                     /locus_tag="IVK51_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP6429746.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52693..54858)
                     /locus_tag="IVK51_000120"
     CDS             complement(52693..54858)
                     /locus_tag="IVK51_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429747.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55118..57412
                     /locus_tag="IVK51_000121"
     CDS             55118..57412
                     /locus_tag="IVK51_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6429748.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57405..58070
                     /gene="pgmB"
                     /locus_tag="IVK51_000122"
     CDS             57405..58070
                     /gene="pgmB"
                     /locus_tag="IVK51_000122"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6429749.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58063..59070
                     /locus_tag="IVK51_000123"
     CDS             58063..59070
                     /locus_tag="IVK51_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6429750.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59092..60111
                     /locus_tag="IVK51_000124"
     CDS             59092..60111
                     /locus_tag="IVK51_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6429751.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IVK51_000125"
     CDS             complement(60392..61759)
                     /gene="nhaC"
                     /locus_tag="IVK51_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6429752.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61868..63289)
                     /locus_tag="IVK51_000126"
     CDS             complement(61868..63289)
                     /locus_tag="IVK51_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6429753.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IVK51_000127"
     CDS             complement(63532..65409)
                     /gene="tdc"
                     /locus_tag="IVK51_000127"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6429754.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65700..66956)
                     /locus_tag="IVK51_000128"
     CDS             complement(65700..66956)
                     /locus_tag="IVK51_000128"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP6429755.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            67536..68837
                     /locus_tag="IVK51_000129"
     CDS             67536..68837
                     /locus_tag="IVK51_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6429756.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="IVK51_000130"
     CDS             complement(68930..70318)
                     /gene="ltrA"
                     /locus_tag="IVK51_000130"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6429757.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(70805..71404)
                     /locus_tag="IVK51_000131"
     CDS             complement(70805..71404)
                     /locus_tag="IVK51_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6429758.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            71658..72539
                     /locus_tag="IVK51_000132"
     CDS             71658..72539
                     /locus_tag="IVK51_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429759.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            72552..74162
                     /locus_tag="IVK51_000133"
                     /pseudo
     CDS             72552..74162
                     /locus_tag="IVK51_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
     gene            complement(74239..74898)
                     /locus_tag="IVK51_000134"
     CDS             complement(74239..74898)
                     /locus_tag="IVK51_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6429760.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(75181..75846)
                     /locus_tag="IVK51_000135"
     CDS             complement(75181..75846)
                     /locus_tag="IVK51_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429761.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(75993..76598)
                     /gene="dhaL"
                     /locus_tag="IVK51_000136"
     CDS             complement(75993..76598)
                     /gene="dhaL"
                     /locus_tag="IVK51_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP6429762.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(76626..77609)
                     /gene="dhaK"
                     /locus_tag="IVK51_000137"
     CDS             complement(76626..77609)
                     /gene="dhaK"
                     /locus_tag="IVK51_000137"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP6429763.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(77611..78006)
                     /gene="dhaM"
                     /locus_tag="IVK51_000138"
     CDS             complement(77611..78006)
                     /gene="dhaM"
                     /locus_tag="IVK51_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP6429764.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            78160..78480
                     /locus_tag="IVK51_000139"
     CDS             78160..78480
                     /locus_tag="IVK51_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP6429765.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT        23199 a        15981 c        14029 g        25298 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt acgtttgaat
     1501 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1561 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1621 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1681 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1741 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1801 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1861 tagtcttcgt tcttcttcta tcgtcgttac tggttctgct tttatttcat taaagtcgat
     1921 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1981 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     2041 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     2101 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     2161 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     2221 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     2281 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggatat
     2341 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2401 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2461 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2521 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2581 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2641 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2701 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2761 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2821 ataattagtt aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2881 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2941 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     3001 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     3061 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     3121 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     3181 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     3241 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3301 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3361 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3421 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3481 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3541 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3601 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3661 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3721 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3781 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3841 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3901 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3961 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     4021 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     4081 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     4141 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     4201 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     4261 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4321 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4381 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4441 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4501 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4561 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4621 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4681 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4741 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4801 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4861 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttagttg taaagattcc
     4921 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4981 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     5041 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     5101 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     5161 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     5221 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     5281 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5341 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5401 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5461 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5521 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5581 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5641 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5701 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5761 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5821 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5881 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5941 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     6001 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     6061 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     6121 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     6181 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     6241 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6301 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6361 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6421 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgtcttagaa
     6481 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6541 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6601 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6661 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6721 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6781 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6841 tctctcatta cgttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6901 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6961 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     7021 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     7081 caattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     7141 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     7201 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     7261 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7321 tcttttcgac ttcgcgtcaa caattggttt acgcgctttc tttttgggtt attgacgtcg
     7381 cttttaggag atcacttaac aagcatcggc gatttttttg ttttgaaatc ttcggttatt
     7441 gaaccaggag attcattgca gtattttcac attgtcttat ccgctttttt atctgcttta
     7501 ttagcttttt gtttaaaaaa atcgttagag acttggtttc acactgtgga ccgccgtatg
     7561 atcggtacga tcgggacatt gatattattt acatactata tcattatttt ttatacccgt
     7621 ttttctggtg aaacacctga tgtcttagct ttgaatacga gttttttcat tctatatctg
     7681 tgtgttggcc tgatcatctt ggtttattat tggaaaatct ctaataagaa attagctgat
     7741 cagttattag agcaacaagt caggatgcaa caggattata tcaaagatct agaaaaaaac
     7801 tatcaagact tgagagaatt caaacatgat taccaaaatc tgcttttttc tcttcacagc
     7861 tatatttcag aaggcgactt agaaggatta cggtcttatt atgatcaaac gatattacct
     7921 acacgccaga tgatgaacct ttttccggcc aatctaagta tgttggataa tatgaaagtt
     7981 cctgagatcc gtagtttact gtcattaaaa ctcatgatgg cacaggaaaa agggttgatc
     8041 gtccagcttc attttcctga gccaatcgaa ctggatacga aacatacggt aaaccttgta
     8101 cgtatgcttg gtattatctt ggacaatgcg attgaaggag ctagtgctgc aaagaagaaa
     8161 aaaatcgaat tgaccatact caagaagaat cattccgtga tgattcgtgt gatcaataca
     8221 acaacaaata cattaccgtt aaaccagttg aaacaaaaag ggttctcaac aaaaacaaat
     8281 ccaaaaaacg aaggccttgg cttgtttatc ttggatgaat tgacaaagac caatccttat
     8341 cttttccttg aaacatcaat tgaaaaccag cgtttttctc aaacgattta tatacaaata
     8401 aaataaagga gtaatgatat gattcccatt tatatttgtg aagacgatcc aaaacaatta
     8461 gaagttttat caacggtgat caaaaataga atcatgattg aaaacttaga cagttatgtc
     8521 catatttcta cctctgatcc ggaagaactt ctcaatgcag ccaagatcag aacttcttct
     8581 tttggtattt actttttaga tatcgaacta gaagacagtg aaatggatgg tgtagcgatc
     8641 ggtaaattga tccgtgaatt agatccttta ggaaaaataa tctatgtgac ttcccatacg
     8701 gaaatgtcga tgaaaatctt aaaaagcaac atcgaaccga ctgattatat cgtcaaagaa
     8761 gatctttatg atttaaaaga aaatgtcgag catatcctga gcaaaatttt cgaagatttt
     8821 caaatgtccc aaatccaaaa agatatcttc aaaatagagt ttaatgatga gatcaaattt
     8881 ttaccgatca aagacatcca atatttttct acttcgctag gagcgccaca taaactggaa
     8941 gtccatttga ctcatgccca attacaattc tatgagaaga tcaaagaaat cgagaaaatg
     9001 catcggaatt ttatccgttg tcataaatcc tatgttatca atacacaaaa tgtgcggtcg
     9061 attaataaaa aaaccagaga agtcacgttg gcaaacggcg agaccatccc agcctctatc
     9121 cgtggattaa agaaattgat ttcataaaaa gaaccgaata gtttggaccg actaaatgac
     9181 cagccgatcc aaacccttcg gttctttttt gcctatcagt gcaaaagcat ttcctgtgca
     9241 atttcacgtc catccattgt atctggataa aatgttggcc aattctccat ttctttcagc
     9301 aatacttctt gactttcatt tcctgcatat aaatgaaaat gttcggagtc agccggtgca
     9361 atcgtgtgat cactgaattg aatatatcta aaagcgccac tttccttatc ttctgcttcg
     9421 aatagatacc ttacgccacg gtttcctgcc tcgtaggtca agatttcttt ccctacataa
     9481 cgataatttg aacgtttcgt tgttccattc ttaaacgtaa atgccatctc tttctcactg
     9541 atcttgattg aagcaatatc tgtttgatag cccttcgtat aatattcttt atattcttgt
     9601 gctgtcttat cctgattcaa tttttcttta tattcaaaaa caggatcaaa agtcccatct
     9661 tctacatacg tatctactgt ttgccaagtt cctgcccaat ctgaaagaga gcgatcttgt
     9721 acatcctcgt cttcaaaata accgttgtag acgctctttt cttttttggg ggtcaaagta
     9781 tcttctagcg ggttttctgt ctctgtcgtt tttttcaacg cttcgaggtt tgcttccatg
     9841 atagaaagat agttttctcc attttctatt tgttcattcg tcaatccttc caaaggattc
     9901 aatacttcta aagatacacc tgcttcgttt gctaacgttc tggcaatact atctttggcg
     9961 ttttcttcaa aataaatata gttgattcca tgttctttaa caaagtgttt cagttcagca
    10021 attctcgccg cagacggttc ttcactagaa gaaagaccag caatcgatac ttgattcaat
    10081 ccatattcta acgctagata gttgaatgca gcatgttgcg tgacaaaaga tctttgtttt
    10141 gcttgcttaa aggtttcttg ataaagctga tggagtgctt gaagtttctc ggtataggct
    10201 gctgcatttt ttgtccatac ttcctgtttt tctgggtaag cttcaatcag ctgatcacgt
    10261 atgtttgcta cctgcttgat tgctaaactt ggagctagcc atacatgtgg gtctagctca
    10321 tgtgtatggc cttcttcccc gtgttcatgg tcatgatcat gtccttcttc acttcccgga
    10381 agtaaaagca taccttctgt cgcttttatc gtatggacat tgccttcttg taatgttttt
    10441 tggattgcgg ggacccatgt ttccatgttt tcatcattat aaacaaagat atctgtatct
    10501 tggatctttg ccatgtcttt agcagaaggt tcataatcgt gggaatcagt ccctgctgga
    10561 atcaataact ctacttctcc ttcatccccg acgattgctt ttgtaaaatc atacatggga
    10621 taaaaggtcg tcatcacgct caatttttct ttactgtcct cggctttccc acttgccccg
    10681 caagcagtta acatacctgc taaaaccagc atggccaata tacctatcaa tcgtttcatt
    10741 tatgacactc ctcttctcat cttctttcat ttgaatgttc cgattcgctt ttcgcaaacc
    10801 gtaatagtta cgttttagca acaggattta atttatcata tggttaaaag aactgtcaag
    10861 cgttttctta ttttaattga tgagaaagtt tatatgaatg attgacgata tttttgcaat
    10921 caacctctat cattttcctt ttatacccgc ttttgtctat aatgaagtta tagataaagg
    10981 aggaaaaaga gtgaaaaaag aaacagatac aacgaatcgt ttgatccgct ttttaggagg
    11041 aaaaacctct tactatgttt taggaatagt tatcttactt gcgctgatca tttatttttt
    11101 ccatcagatc tcgtttatat tcaagcctgt cggaatcatt atcgctacta tcttgccccc
    11161 attattgttt gcactgatct tgtattatct tttggaacca gtcatcaaac gtttgagcaa
    11221 gcacctttcg aggaacttgt cagtcattat cgtttatgtg attattttat ttttattggg
    11281 gcttggaagc atatggctta ttccttttct tgaagaacag gcaaagacat tgatcagtag
    11341 tttgccagat ttgtttcaag attttcaaga gagtctccga caatttttgg agaaaacacc
    11401 ttttgccgat tcatttaatc aatttttcgc ttctattgac gacgtgacgt ctgatatagc
    11461 tggatttatt ggaaattatt gggaaagtgg cgcacaacgg atcagcaaca ttttttcaac
    11521 tgttacagct atttttatta ctttatttac tggtccaatt gtcgcttttt tccttttgag
    11581 agatcctcaa aaattttatc agtcagtttt agcgattgtc ccaccagcat ttcgtacaga
    11641 ttttaaaaat ttgacaaaga ttgctaatca acaattgggt tcatttctaa aggggcaaat
    11701 cattgcttca tttatccttg gagcagtcta ctgggtttgt ttcttactga tcggattaga
    11761 atttgctagt gttttagcta tttctgctgg gttgttatgt atcattccat atattgggcc
    11821 gtttatcgcc ttctttccag gtttgatcat tgcattccaa gactctactt ttatggctgt
    11881 caaatttgtc attgtctggt ttgccgtaca acttctccac ggtgacttgg tgattcctcg
    11941 agtaatgggt gatcgtttac agatccatcc gattacgatc ttaatcgttt tacttgtcat
    12001 gggtgatcta atgggaattg tgggcgttat ttttggtatt cctatctata cgttagtcaa
    12061 attactcgtt acttttattt tccgtaaatt caaacaacgc tacaataaat tttatggcga
    12121 taaaggagag tatgaaaact cttcattttc tgaagaagat tattttaaat aaaacacaaa
    12181 acgtcgatct tacgagaata gctcgtagga acgacgtttt gtgtttttgt tttaattttt
    12241 cagcaaagca attgcctggt atggttttag ctccagactt tgatgaagaa cagtatcgga
    12301 ataattatcg ataagaatat tggcttctgt gaatccttct gggatggcga tccttgtatc
    12361 tttatcatag aaatttgtta atacaagcag ctgctgtcct tcaaattggc gaagaaagcc
    12421 ataaacttcc ggatgatttg cttcataagc atgataatct ccttgtgcaa cgattggata
    12481 tgtttttctc agccgaatca atttcttgta ataggacaaa atagaatctt cagaagatag
    12541 ttctttttct acattaatct cttctagctt gcctccaact tttaaccaag gtttccctgt
    12601 agtaaatcct gcttgttctg tgtctgacca ttgcatgggt gttcgagagt tatcgcgaga
    12661 ttttgctttg atccgacgaa acgcttcttt ttctgtgaat ccttgttcaa ggagtaactt
    12721 ataagcattc aaactttcta cgtcaatata gtcggaaatc gatgaatagt ctgggtcgat
    12781 catgccgatt tcttctccca tataaatata gggcgtccca cggttcaaat ggatcatggt
    12841 tgctagcatc tctgctcctt gttttcgata ctgatcgata gctacaaatc gattgagcgc
    12901 acgtggctga tcatgattat taaaaaatag agcattccag ccgttaccct cactcatctt
    12961 ttctccccat gtatgaaata gtcgctttaa ttctgcaaaa tcaaatggag gagtagacca
    13021 tttattccca ttttcataat cgactttcaa atgatggaaa ttaaagacca ttgataattc
    13081 ttctctacta ggttctgtat acaagataca attttcgatc gtcgtagagc tcatttcacc
    13141 gactgtgatg atagtggaat ccgtcccaaa cgttgcttgg ttcaattctt ttagatacgt
    13201 atgggtgatt ggtttgtccg tatattcagg tttgccatca ttttctttat tatttttcaa
    13261 ttgttcgtct ttaccgataa cattgataac atcgaaacgg aatcctttta cccctttttc
    13321 catccaaaaa cgaacaactt tgaatagttc ctctcttact gcttggttcc gccagttcaa
    13381 gtcagcttgt gtccgatcat ataagtgcaa ataatatttc cccgtatctc caaatggcga
    13441 ccaagcactt ccaccaaatt ttgataccca atccgttggc tcttccctta aaatgaagaa
    13501 gtcctgataa tgctggtccc ctgctagtgc ttttttgaac cattcatgtt ctgtcgaaac
    13561 gtgattcagc accatatcga gcatgatttc tatccggtat ttctttgctt cggctattag
    13621 ctgttcgaat tcctccatcg taccaaagat aggatcgatg gctgtgtaat cagagatatc
    13681 ataaccgttg tctttttgag ggcttggata aatagggttt agccaaatca tgtcgatgcc
    13741 tagatcattc agatacggga gtttttcttt gattcctttt aaatctccaa ttccatcacc
    13801 attactgtct ttgtaagatt tgggatagat ttggtaaatg actttgtctt taaatgtcat
    13861 ggatatttac ctccattcaa ctgatttatt acctaccaca acttatatat acaagttatc
    13921 atttattatt ataactgaac cgaaagcgtt tgtaaaattt atctttatac attctaagag
    13981 gtctgttgcc caataaaaaa caagagatca gacatgtatt tgtctatcct cttgtttaaa
    14041 atagcaaaat attcttgttt cttctataaa tggtgttcaa aagcttttat ctacggcaat
    14101 cagtcttctc aaatgtaaaa catgaggagc taattttatt tgagtattct ttttgctcga
    14161 tgcaggacgg cttttttaca acctctgatt catggtgttc aatcagctgt ccttcggtat
    14221 tagctcaaaa aacggcaaaa tatgaaaaac tattttgccg tttgtttctc taatactcag
    14281 gtcaaaccgc tgatttcaca acctctgttt ttattcattc gatttcaacg catcaatcat
    14341 atcgatatgc tttaatttga aatgggtaac aatcatcaca ataaccgtaa atacgatagt
    14401 taatcctgct gataagacat aagcccacca tgtgatcact aatggaaaga tgacattctc
    14461 catagacgct tgttgcaaga tgaagtcagt caatatatat cctacaccaa atcctccgat
    14521 gattcctaaa agagtaaaga tgatattttc tcgaacaatg tacatcgtga cttctttatc
    14581 aaaaaatcca aggactttta ttgtagataa ttcgcgtaca cgctcggaaa tattgatatt
    14641 agttagatta tacaagacga taaaagccag cagtcctgat aatacgacaa agattaagac
    14701 aaccgagtct aagttcgcca ttgattcttc ttgcgtttcg atttgtttgg acatgaaaga
    14761 cgtatttgtc acagcgtctg tggcaagcag tttctcggaa agcatttctt cttcttttgt
    14821 tgtcatctta tctgtacgca gcaaaaatgc attatccatc atcgattgat cagtaactga
    14881 agcataatat tcaggggaca tataaataaa gctacctaga tagtttttca cgatttcttt
    14941 tacttcgact ttccattctt tttgatcttc atcataaagc gaaatatgat ctcctgtaga
    15001 taagtcaaat agttctgcta ctttttgcgt aatagcgatt cctgttttag gcaaggagcg
    15061 tgggttctct tcaagatcag ttaaatggat aaaacgatcc aaatcagctg ttttacttgg
    15121 tacgatcact gtcagacttt gcgaagcatg tccttttttt ctcaattcta cggtttgagt
    15181 atgaatagct aggatctcat ttactttttc ttgcttctgc agcatatccg ctgctttttg
    15241 acgactatct tccgtggatg aatccaatgt aacgattgct tgatagtcaa tgatcgttcc
    15301 aaattgtttg gctgaaacag aacttagaga atctttcaat cccacgcctg caaccatcag
    15361 cccggcacat ccagcaattc cgataatcgc cataagcatt cttgacttat atcggaagag
    15421 atttcggtaa ctgatttttt gattaaagct aaggcgtgac cacagcggag tgatgtattc
    15481 aagaaaaatg cgtttccctg gcttcggtgc tttcggttga agcaatgcag caggccgttc
    15541 tcttaaatct ttgaataaaa cgatcattgc agcgccaaat gcggcaatga agaatgcggc
    15601 agctgcttgt aaaatcggcg gccagtcata aaaaataagc gcactcccaa tatcataccg
    15661 ctcgttagac agctcaaaaa tgatccttgg caaaagattc gttcctaata tcgttcctaa
    15721 aacaatacct atccctgagg ctagacttgc atagattgca tatttttttg cgatttcaac
    15781 tttcgtataa cctaacgcct ttaatgtccc gatttccctg cggttttctt ctaccatacg
    15841 tgtcattgtc gtaaaggtga tcaatgcggc aataaagaaa aagaaaacag gaaaaacatt
    15901 ggcaatcgca gcgatacgat cagacagatc accaaattct tgaaaaccga cattttctga
    15961 acgttcattg tataggtatg atggtttagt taaattttca attttttctg catttcgatt
    16021 caattcttcc tctgcagagg ccagttcacg ttctttctca actaattgcc tttcttgttc
    16081 agcaagttga gcttcttgtt gagataatgt ttgaacagcg attgttcgtt gcgcttcagg
    16141 aagctgctgg atagttgctg actgttgcgc taactgtttt ttagcatctt ctaaccgtgt
    16201 tcttgcttgc gttgtttgtg ctttgccatc cgctattttt tgctgtgccg gttctaattc
    16261 ttcgttggcc gattttttca gctcttctaa gcgttcttcg ggtcgatctt gtaaatactt
    16321 ttctgccttt tcttgatttt gctccatttt atctttgtaa gctttgctgt acgtattcaa
    16381 attctttacg tttgtaaaat caagatataa gacagaataa atatctgttt taaaaacgga
    16441 ttcaggtaaa tagacaaaat aatcgacgct accatttccg acgttcgcgt atcctctctc
    16501 caaattgttg atgaacaatg gagaacggac aaagcccgtg atggtatact ctttttcttt
    16561 caattcatct gaatcaatct gataaatatc accgatttta tacccttcat cttccgcttt
    16621 ttcatccaat acaagctggt ttggtttttc cgggagtttt ccatcagtta attgtagctt
    16681 gttctgcttg ttatttttgt tataagaaaa tatttgaatc acttcgtttt tgccagtatt
    16741 ggaataatag atctgttttc ctgtttctac ttttgcatct gggatctttt ctgcttgagc
    16801 aatatctttt tgtgtcaagc caccagtaga aacgatttgc aaatcagaaa gtcctgctgt
    16861 accaacatag tgatccatcg ttttttgcat gacaggtcca gcagttttga cacctacaaa
    16921 taaaagtgtt cctagcatga caataaggat gattgcaatg aatcgtcctt tagattgccg
    16981 aatttcgcga aaacctgctt taagatatgt tcttgttttc ataaaatccc cctaccatac
    17041 aatctcatca atggatgaag gatgatcatt taattcaaca gaccttactt tagcatcatt
    17101 aatgtgaatg actctatcag cgatgggcgc taaagcagaa ttatgtgtaa tgatcaaaac
    17161 ggtattgccg tgttgtctgc tagcgttttg caataatttc aatacttgct tgcccgtttc
    17221 aaaatccagc gcgcctgtcg gttcatcaca taaaagaagt ttgggatttt tagctagtgc
    17281 acgagcaatt gaaaccctct gctgttcgcc tcctgataat tgggacggaa agttattcaa
    17341 tcgatgagct aggcccactt gcctcaatac ctctacagga tcaagcgcat caggagaaac
    17401 ttccgttgcc aattccacat tttcttttgc tgttagatta ggaacgagat tgtagaattg
    17461 gaaaacgaac cctacatctg ttcgtcggta agcagtaagc tgtttatctg aaaattgcgc
    17521 aatatctgtg tcatcaataa tgatctgacc ttcatcaggt gtatccatcc cacctaaaat
    17581 attaagaatc gttgactttc ctgctccact tggaccaagt ataatcacta attcgccttt
    17641 ttcaattgaa aaagaaatat cgtcatttgc tgtaatggtc gtttctccca ttttgtatcg
    17701 tttgtattca ttttttactt cgacatactt cactctgatt cctccatttt cacgatattt
    17761 ctcgctatat ctatgcatcg aatgcgtttt ctcacactta tttattttac cactgaattt
    17821 ctctagacta aagaaaggaa tcctttaatt tagttgacaa ataaacgatt tatgatttac
    17881 ttacataaaa aacattgtta tttgaaagaa ggaacatcat gaatgattta aaggaaaata
    17941 tgatttttga tttatcaaaa actgcttttg tcgcgatcga catgcaaaaa gagatcgtaa
    18001 ataacggtac actctctcca cacactgcag catctattct gacaaacaat gatttgttag
    18061 taagaacatt gaaacgtacc gctgccttga aagttttagt gaacgtagac atctccactt
    18121 tcccttattt gagtcaacaa actgatatgg gtggaatgac tgcttctgta cctccagaat
    18181 tcactgattt acttttgaag gattctttga aagatacaga taatatgttg actatcacaa
    18241 aatacaatcc aagtgctttt tttggtactt ctcttgatct tcaacttcgc cggcgtggaa
    18301 tcgaaactat cattttaagc ggtgtagcga caacaaacgg tgtatatgct actgcattag
    18361 atgcctttca acacgggtac catatcgtgc ttgctgaaga tgcttgcagt gatcgtgaca
    18421 aagaatccca tcaattgttc ataaaaaaga tatttccgaa aactgcacga gtacgctcaa
    18481 cgaaacaaat aatcgaagct atccaacaaa gcaaataagt actggcacat gtgattttca
    18541 tttacttctt tttgatttca tgggaaaata accacaggat ttagatagga gtatgaacat
    18601 ggtgaaaaaa aagatcacaa tcaaagaagt cgctaaactt tccggcgttt caatcacaac
    18661 tgtctccttg atattaaatg gaaatacgga gaaattcagt ccgaaaactg ttcagaaagt
    18721 tttagctgcc aaagaagaat taggctatca gcctgactat tttgctagac aaatgattac
    18781 caaggaaacg aaaacaattg gtgtactcgt tcctgatatc acgaatccat tcttcagtac
    18841 actgatgcga ggaattgagg atatcttgta taagcagaat ttcgtcacga tattatgtaa
    18901 tgctgattct gatcatcaaa aagaaatcga atatctagcc gagctcacac gtcgaggtgt
    18961 ggatggattc attatcgcga cttctgctgt ctctacagat gcaatcaacg aaaatttgaa
    19021 aaaacaaggc cggcccttca ttgttttgga tcagaaaaaa tctgaaggat ttagtgatgc
    19081 cgtacgaaca gacgattttc gcggtggtta cctagcgggc atgcacttgc ttagtctagg
    19141 acatcaaacg atcgcccttg tctatcctga aaatccaccg gaaaatgtcc acgctcggat
    19201 tgaggggttt aaatctgctc ttgatgttta ccaaatccct cacgaccaac tgatcctctt
    19261 acctacacag ttctcaaaac aaggtggcta ccagatcaca gcagaacttc ttgattctgc
    19321 tgcaactggt gtgtttgctt taaatgatga attagctttt ggattatatc gcggattaga
    19381 ggaagctgga aaaagcatcc ctgaagacta cagcattatt ggctatgata atatagatat
    19441 gtgcgagtat atcaagccga aactgacaac gatcgcacaa ccaatcttcg aattgggaca
    19501 aacatctgcc aagttattgt tagatcggat ccaattccca gaaaaagaat gggaagaaaa
    19561 aagattacct gtacgattag aaaaaagatt ttcaactgca cccttgaaat agctttcaag
    19621 gtgtgctata tttttatcga actattaaaa cgttttacta aaacgtttta aaaaataatt
    19681 ttcccatcaa aataaatgag atagttggag gtttgtaaaa tgaacacggt tacagtaatt
    19741 ggaagtatta atatggacac tacccttcgt ttaaccaaca tgcccaagcc aggcgaaacg
    19801 atgcatgcgc atgagatttt tcacgctggc ggaggtaaag gtgccaacca agcagtggca
    19861 gccaaacgtt caggcgcacg gacatctttt attggaggcg ttggggctga cagcgaagga
    19921 caacagttat tagatctact gacaaaagaa aacatcgata catctgggat agctgaaatc
    19981 caaggcgcca caaccggaca agcaatgatt atggtagacg cagcaggaga aaatagtatc
    20041 ttgatccatg caggggcaaa taatgctttc catgaacaag aagtcctaaa aaacaagcaa
    20101 ctgattacaa acagtgactt tattattgct cagtttgaaa gtagcttaga tgcgacgatc
    20161 ctagcttttt ctattgcaaa agacgcagga aaaacaacga tcctgaatcc tgcaccagct
    20221 cgtgaaacga ttcctactga actacttgaa aagacagata tcattatacc aaatgaaaca
    20281 gaaacagaaa tcattacagg aatcagagtg acagatcata acagtttggt cgcagcagcc
    20341 gaaaaactcc atgaattagg tattggaaca gtaatcatta cactaggcag tgccggcgct
    20401 ttctatcata cagaaaaaga acacggtatc gtccctgctt ttaaagtgga tgccgttgac
    20461 acaacagccg caggtgatac atttattggc gcattaagca gcacacttca gccagacctt
    20521 agtaacttga aagaagcgat cctttatgga aatctagcct cttctgttgc tgtgcagtct
    20581 tatggtgcgc aaccttctat cccttaccgc gaagctttag aacaatcagc agtctaaaat
    20641 taacaatgag aggaagaaac aaacatgaaa aaaggaaaag taatcaattc agatatttca
    20701 cgagtaatcg cacaaatggg gcactttgat aaattaagta tcggggatgc aggcatgcct
    20761 gtcccctttg gtactgaaaa aatagatttg gctgtctcaa acggtcttcc aagtttttta
    20821 tctgttttag aaaacgtttt agaagaactt gaggtacaat cgattgaatt ggcggaagaa
    20881 ataaagacaa tgaacccgga aatattggaa aaaatcaaat tattgttacc agatacaccg
    20941 attagcttta tcccacatga agaaatgaaa aaagagttga ataactgtca tgcatttatt
    21001 cgtacaggcg aaatgacacc ttacgccaat attatattag tgagtggagt ggtcttctaa
    21061 tggaaacgac aatgaatcct gaacagatgg tactgtccga gcagattcat atgctgattg
    21121 aacagttggg aggaaaagac aatattgatt ctgtccttcc atgtgcgact cggcttcgta
    21181 tcaaattgaa aaaacagcca gtagccaatg cagaaaaatt aaaacagcaa atcccttcca
    21241 ttaagtattt cattttactc gatagatatg cagaaataat catggatcag ggattggctc
    21301 ctgcttacaa ggaattgacg cgtcaaatcg atcattcagc tccgaaagaa aagctaaatc
    21361 cgatcgaaaa actatttggt ttgatatcgg aaatctttac gccgatcatt ccgctgtttg
    21421 ccggttctgg tatattaaaa ggtctggttg ttttagcgac tacgattggc tggcttagtg
    21481 aaacaagcgg aacttatcat attttgtctg ctgcgtcgaa tgccgtcttt tattttctgc
    21541 caatctttct tgctttttct gctgccaaaa cgtttaaagt gaatggatat attgcggctg
    21601 tcattgctgc agcactgatt gaaccgaatc tgacctcttt actttcagac agtggcaaag
    21661 cagctactga ttttctgggg attcctgtag tactgatgca gtatacgtct actgtcatgc
    21721 cggcgatttt aggtattttc ttttattctt ttgttgaaaa attcctcaaa aaatatattc
    21781 gcgaaaatat gcaattagta tttgtacctt tactttcatt agttatcacg gttcctttga
    21841 cgttaatggt attcgggcct gcgggtgttt accttggcga aggtttagct gccgcaatta
    21901 cttggatgat ggatattaac ggaccgatca gtggcgcaat catcgctggt ggttggaata
    21961 ttttggtaat atttggtatc caatgggctg taaaccctgt catgatcagt aatatctctg
    22021 cttatgggtt cgaccgaatc gttccgttga caggagcagc caattttgga atggctggtg
    22081 cagctttagg tgtatttctg cgaagtaaac gttcaaaaac ccgttcgatc tccggttctg
    22141 cttttgcttc gattttactt gcgggagtga ccgaaccaac tgtttacgga attgcgatcc
    22201 cattgaaaaa accatttgtt gctgcttgca ttggtgcagc tgctggtgga gctgtaatgg
    22261 gatttgccca agtgaaagcg atcgcttttg tatttggtag tctgaccaca ttgcctgcat
    22321 tcatttccgg tacttttttc tggtacttag caggactggc tgttagtcta gtcgtagcaa
    22381 tgatcactac tctcgttagc gggtttgatg aagacctaat gtcttatgaa tagttaatga
    22441 aaaagactga agtatgagat gtttatcaca tatttcagtc tttttctttt taggatcatt
    22501 atttttataa atttttgtga tactttccaa actttggtat aatttacata tgcataacca
    22561 tttttatccc cacttgttta tccccaaaca aaggtggtct atgcaataaa atgaacgtca
    22621 aagaaaaacg aatgaatgct cgttgcttat ttacaaattg ttttatttag aatacacaga
    22681 cagatttatg aaaagaaaaa gcttttcgac acgattatct gttccaaaaa atgtctaatg
    22741 aatgctgaat aagacagaaa atcaatgaaa ggaaaaggac tagtttatta tgaaaaataa
    22801 aaaaacgata cgtttgctag ctgctacttg gctgattgct ccgtctgttt tgtcgaccaa
    22861 taccgtattt gccgtagata atcaaatagc tgatcaaaca gtcacaacag atacgactac
    22921 atccagctca gctactagtg aaacaacgga ttcttttaca gaagacccaa ctggaacaac
    22981 taactcaaca acaggatcga ccgagccaac tgaaattgat ccggctgatc catcagaaaa
    23041 tgaaaagcca gccgagacat attcccttac aattgctcct tctttgattg gtaagatcca
    23101 actgactgct tcgacagcgg attcccctga acagcaacct ttggatatta cggaaagtgg
    23161 aacggtagaa ggtttattag cggaaacgaa ggtaacttac actattacgc cagctgaagg
    23221 tgaacaaatt gcttcagtcg ctgttaatgg tgccaaagaa gtaaattatg atgcaaagac
    23281 ctttacagta ggtacgaaca atttatctat tactgcagaa tttaaagctg gaccggatac
    23341 ttcaacagag aaaccaactg atcctagtga agaagtccct agtacaccag atccggaaac
    23401 gccaaacaaa ccagatgtgg gtaataacaa caataataat ggttcaaata attcaaacaa
    23461 taataacaat aattcgaaca gcgggaattc ttcgaatcaa acaaacccat ctggatcgaa
    23521 taacaatgga aatactcaga gagaaccaaa ccatccaaac aatggttcat cgaatggcgg
    23581 aacgaacaat gcttcatctc gtccaaatac agcaaatcaa agcggtatag agaatccatc
    23641 ttctgcttct tctgattttg ttgtaaaatc accaattgat gctgtattgc ctacaaatac
    23701 aacgaatgtc caacaagcaa tcgttcgaga agcattcaag catcttggaa aaccatatgt
    23761 atggggagca aaaggtccaa atacttttga ctgttctggt ttgacttatt acgtatatat
    23821 gaaagcaacc ggtcattata tcggtggctg gacaggcgaa cagcaatatg caggtacgca
    23881 gattccagta agtcaggccc aacctggcga cctcgtcttc tggggacctt cttccggtgt
    23941 tactcaccat gtaggtatct atattgggaa cggacaattt atccatgcgc ctcaaccagg
    24001 agataaagtt cgagtaactt ctatttcaga tttcacgcct gattttgctg tccgagtaaa
    24061 cttagcaggt ttacctgctg cttcaggttc gctgacaggc ggctcatcta ttttagatgg
    24121 attagatggc tcgttccatt tcagtcaaaa ccaaacaact gatcagttct tgaaaaagat
    24181 tgcagatgat gcacaggaaa tcgggcaaaa agaaggaatc tacgcatcag tcatgatggc
    24241 tcaagctatc cttgaaagtg gatctggaaa cagcttatta tcaagcgaac caaatcataa
    24301 cctttttggt atcaaaggat catataaagg aagcagcgtg accttcaata cacttgaaca
    24361 agatagttcc ggacaaagct accaaatccg cgcacaattc cgtaaatatc cttcttacaa
    24421 ggaaagtttg gaagactatg ccgacttgat caaaaatgga ctgactggca atcctgattt
    24481 ctacaaacca acttggaagt cggaaacaaa agactacaaa gaagcaacca aatatttgga
    24541 aggacgttat gctacagatc gccaatattc acaaaaattg aacgcgatca tcgaagcgta
    24601 cgacctgaca aaatatgatg aaccgaaaaa agaagcagca acaactgaag aagcaaacgc
    24661 tgaaatcctc aatatgtcta agcgattcga tgtgccaatc aaatggacaa cagatggcgt
    24721 gccaatcaca ggattagaat tttcaacgat aaaacgcgtc aacaacgctt cttccatctt
    24781 ccgtgtcgct tcaatctggg atttgtggaa caattttacg gcaagacaga taccagaaac
    24841 aactactcgt acagttaaag ctgcaactgt tcctgtacta accttgttaa gtttagacaa
    24901 atcattctgg cttgatctgc ctttaaagaa atagaataaa ttaaaaaggg gatgtgacac
    24961 tgtcacactc cctttttaat ttattctatt tctttatatt tattttgtat tccgttacaa
    25021 actcgcgtac ttggttaggt tccagagtga catctccaaa actttcaagt cgctccgctc
    25081 ctggcagttc ttgagtctca aatgtgatac cgccatgatt tactaattta ttcccgcgca
    25141 tttccggtcc atcttctcca aaatttgctg taaaaatcac gattcctgaa gcatctgttt
    25201 ttacttggat cgatactttt tcatctgggc ttgtcaattc cgcagcattt agtaaaccag
    25261 attgtttttt caacaaaaat ggatgatcga tgccgcccac tatcattgat tggcgaaaat
    25321 cactggcaaa gacctcacct aattttttag gacgttggaa gtcaaatgct gtgccactta
    25381 ctgaaactac ttctcctgta ggcgttgtat ctttattgag cgtagcaaat aatcggctat
    25441 tcaaccaaag tatatgttcg tcgattgact gtgctggatc accggttaaa ttgaaataga
    25501 catgatttgt aggattgaat actgttttgc ggtcactgat cccttggacc gtcactcgcc
    25561 atgtatcatc gttcgtcagc gtataccgta cttctacatc taaagttcca ggaaatccgt
    25621 tttccatatc tgggctagtc tgatagaaaa tgaccgatgc ttcgttttca ccattgatga
    25681 ctgtataatg ccactgcttg gcttcaaagc taggtgcacc accatgtaaa ccaatgacca
    25741 gtgtccgggt ctgtttttac ttcgaacgtt tcatcatcta atttgaatgt gccattcgta
    25801 atccgtccag ccatacgtcc gattgaagca ccgatataag ggtctttttc tagatattct
    25861 tttgcttcat caaaacctaa gatcaactct cgtttttcat tatcgattat tgtttgaaaa
    25921 tgaacaaccc gtgccccaag atcactgaca gacaaagatg tcccattttg attcgtgatc
    25981 gtaatcaagt gatagttttc gccaaaattt tgttcattga tttccatgtt ccttgcaccc
    26041 tcttttcttt catttgcact gattccagca tacatggaaa ccgttttctt aacagcttat
    26101 tccgtgagta acgagaaaaa atttactata ttctaataaa agaccgtgaa aaaaaggttg
    26161 tgacaaaaat attgtcacaa cctctttttc cgaacaaacg gtgttcaaaa gcttgcatct
    26221 gcggtaataa gcccaaccaa acgaaaaata tgaggagcta tttttgcttg gctgttctta
    26281 ttacttgatg caggacagct ttttcacaat ctctttttca atacgattcc agtatataac
    26341 catgtcgtca gtacatagta gatgaaatac aatacgaaga atatcgtaaa cggaacccaa
    26401 atatctttgt atggattttg catgaataga ccaaacattt gtaatccgaa taagacatgg
    26461 actacaccta agattcctgg tgctaagaat aacacgccta cttctctacg gatcgattgt
    26521 ttaagcaacc cgcgtcgtgt tcctatcttt tcaagcattt cataacgagc aatgtcactc
    26581 ttgcttcctg acaagatctt gaacatcaaa cagctagcaa gcatggtcaa gaaagctaaa
    26641 cctaggaaga atcccataaa ttcaaaacca gagaatagac cattatacac ttcatacatt
    26701 tcatatttct ggttgctgta ctctactttt tgcaaagatg gattgttcat tttattttga
    26761 gcaacaagtg tttcgattgg tttcaggttt gcttggaagt cttttacctg aactagctgt
    26821 aaagtggatt gtggcaagtt cagttgatca aattctgatt gactgactac cttgatttct
    26881 ttttcttgct gttccggcaa ttcatagctt cgaagttcct cttgtacagt cagttcattc
    26941 atttttttag caggaacttt ttctttcgtg atcttcggcg gtgttccatc ttcatgtttt
    27001 atcatgatca atggttgagc cgcaaattca cttgcattat agtaaatcgt tcccgcatct
    27061 tctttttgag tataagaagc agaaagtgtt ggtgaaagtt ctttgatttt ttgctgatct
    27121 tctgcccgag gattattcat cactagatca tatccagaat tgttttctgt caagtctgca
    27181 atttcatttt taaaacctaa tcctaccgtc aaagcgccca gtgccaaggc aaacagcata
    27241 gcgaccattg agagcatttg tgtatattca cgaatacgaa agtttaactg tgacaatgtg
    27301 aaattgttca attttttcaa tgaaatctgt tctgatttct ttagtaatga taaaaagaaa
    27361 atgatcacag aatggaaaat gaaataagtt cctaatacga tcgtaactaa ggctacccct
    27421 ataccaaaga cttgataggt ccccacgtgt ttcatcatat agtaaccgca gcctagaaaa
    27481 gcaattccca gtactacttc aaggaaaaac aagaatttat gctgcttgat tcttgttggt
    27541 gtctgatccg cacgaatcaa tgctaaaatc ggttttttga caatcgaaaa agcattgaca
    27601 acagcagcta ataaaaacaa aacagcaaaa aacaaaacag tgatcaacaa cccgtgaata
    27661 ctaaaaggtg taaaatgcga aatattgaga tctaactgat ccactaataa tcggtggacg
    27721 atacttgtca atcctacacc gacagctgaa cccactaaag tagctgcgat ccctactaag
    27781 aatgtttcag aaaaaatcat ctgtgcgatt tttctgcctt ttgctcccag catcataaac
    27841 attgcatagt ctttttgccg catcgtcatc aaaaaggaat ttgcatataa aatataaacg
    27901 aatgtgatga tccctagtaa aacagaaccg aatcggaaaa tgataactgt agccgcaacg
    27961 atcgtattac ttttcaaaaa agcttcgttg cttgccattg attcaaacat gtaaaaaata
    28021 gcagatgcca taactaatcc tgagaataac accaagtagt ctcttaaacg gctcttgatc
    28081 ccagtaagtg ataatttcca aagcatagtc aatctccccc tactgttcca agtttccaag
    28141 aatagatagt atttctcgat agaaggaatc acggctttga tctccacgtt tcacttcttg
    28201 atggataccg ccatccttga tgaataaaat acgttggcaa taacttgcag aaaacggatc
    28261 atgcgtcaca agaagaatcg atacatgatc ttttgtattc aattcgtcca tcgtatctaa
    28321 aagatcccgg gcacttttgg aatccagcgc acctgtaggc tcatccccta atagaatcgt
    28381 tggttgtgtg atcaatgcac gtgcggcggc gacccgttgt ttttgtccac cggaaatctc
    28441 agaaggatag ctttccaaaa tatgagagat ggataaacgt tctgcaattt tttggacacg
    28501 ttgcttgatt tcttttggtc tgacgccttg taaagaaaga ggaacagcga tattttcttg
    28561 tgctgtcaag ctttctaata aattgaaatc ttggaaaata aagccaatct cattcgcacg
    28621 aaaatctgct agttgatttc cttttaatgt ggtcacatct ttttgattga ttcggatatg
    28681 cccatctgtt ggtttatcta atgtggataa aatattcaaa agtgtcgact taccagaacc
    28741 agaagcgccc atgatcccaa taaattcgcc tttttctaca tcgaagctga tgccgcttaa
    28801 tgcttgcgtc tttttctcat tgttttttcc gtatactttt gttacattct gtacttcgac
    28861 tattttgttc atttctttcc ctacccttta attagttttt ccagtgctgt tttcggcacg
    28921 tctttttttt cgacttcttc atacgaagtg acacctttat ttttgttcca ggtgattttt
    28981 aaatatgctc ctttacgaag cggtcgagtc aattgtccct tgaagtccag ttctttttcc
    29041 ttaccctctt tcgtatagcc aggcaggata tatctatagt tgactgtttc ttgtccagag
    29101 tctgattttg aaactgtttt tgtaccatct gtcgtaattt gtacatagta ttcttctcca
    29161 cccataacta cgtaatctgc gattttcacc cctgcaagta ccacaacgat gaaaacaagt
    29221 agaccgatca gaagtttttt cactttttcc ccttctctct tactttatat actgattata
    29281 gaaaaaagcg aacaattctg ccttcgaatc aactatcatt cttctttcaa ttttgtcatt
    29341 ttctttaaaa caactaaaaa aagagttcaa gacaataatc cgtcctaaac tccttcttcc
    29401 tatcttacaa aaagtatgtc acttacggtc tttcatgtcg cctgtattga taacaggttg
    29461 cgttcctttg tctgtaatga ttgagacaag taaattattg atcaattgga ctttccgatc
    29521 atcggtgaaa ttgatttctt gtccatcttc gatttgctct aaggccattt gagtgattgt
    29581 gacagctcct tcaacaatgg tttgtcgagc agacaaaatg gcttttgctt gttgacgttg
    29641 gagcatggca ctagcgattt ctgttgcata ggctaaatgg ttcaagcgtg tttcgattac
    29701 ttcgacacct gctactgcta aacgctcttg cagctctttt tgcaactcgt cagacactgc
    29761 cgtcgtgttt cctcgaagcg tcaaatcatt ctcattgaat gtatcatacg ggtattggct
    29821 agcgatatgc cgaatggctg tctcactttg gatttctaca aaatcttgat aatacgctac
    29881 atcgaataat gcctttgctg tatcaacgac tttaaagact actactgctg agatttcgat
    29941 ggggtttcca tccaaatcat tgacttttaa aacagaacta ttgaaattcc gaactttcaa
    30001 cgaaacagtc attttttgtg ccaaaggtat cgtaaggaaa aagccatttt ctcgaatcgt
    30061 acctaaatat tgtccaaaaa acaaaattac ttttgcttga ttaggactga ctacagttgc
    30121 cgaacttaac aacaataacg tgatcaacca aagaaaaata cttaaaaaaa gtgcccaaac
    30181 tttcgcttgc cacataccta aatagaagaa aaacaatcca actaaagcta atacgaccaa
    30241 tccgacaatc cctgcataac cattgatata aaatgctctt ttttctctca tgctacttcc
    30301 tgcgctcctt tatccttcaa taaatatcta atgttacttt tttaattttt ttttcgaata
    30361 cgcgccatta caccttctag ttctaactct ttgtcttcag tgatgtacca accgcttgcc
    30421 tgtgtccctc cggctgcctg cggtaaataa tccaattttg ttcgaaccca gtgcttttct
    30481 tctgtaaaat agtcttctac caagacctct agatccatcc ctgctgaaag aggcaaatct
    30541 tcccgaacag atttaaagat aaatcgttcc ttcttcccat catacttcaa tattcctgta
    30601 tcgataatcg gtaaagaata ataatccagc tctttcttca ccgtcaataa atgtttgaaa
    30661 acttcatggt acacttgctc taattggcga ccttctgaat tgttcgggta attaaccatc
    30721 actttttctt tgtctggatc atagaaggtt tggcttagtt gagtgatata agtatgtaat
    30781 ctttccattt ctgtttttaa cttttctctt tgaccttctt ctaccttcat atactctttc
    30841 accctgtttt ctttttagat attttctttc ttattttact atagttatgt gcgttaagcg
    30901 atgaaaaaca gcaaagattg gaaggatctt tgctgttttt tcttaaaata tccagttgag
    30961 tacaggatca atctgtttca ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt
    31021 tagcagtgat ctttagcttt ttctgtcctt gttctaacaa agtatttgtt attggctggt
    31081 agtctttttc aatataagat ttaccagacg tttggatagt atagccactg ttttccatct
    31141 catgcaaaga taaattgcca ccgtttagga tgacttttcc ttctttgctg atcaaactgt
    31201 cttctagatg gaagttgctg ttttcacctg tgacgatcaa gttatccaca ttggcctcat
    31261 ctaatcgaag tgagcctaat tcatctttcg cagttattgt tcctcctgta aattcattga
    31321 acgataccca gccatcatgt gtcttagtat tcactttttt gattgatact tcgttgatat
    31381 caattgcact attattccct tcgattgtca attcagataa ttcatgatct gcagggacaa
    31441 caatagtgat ttgtgcactt gtgtcttcta aactaatgat tgcacctgtt gattttttga
    31501 ctgcattcac agtcaacacg ccatttttct cctttatttc tggcttttct gtcttatcat
    31561 aagtcgtttt tatctcaaaa tccaatccgc gtatgaccga aactgtttga ttttctgcat
    31621 ggatgacgat tttgtttgca tccttgattt tttttgtctc agtaaccata tcgatcaacc
    31681 gaggaccatt atcccaagtc agactctttt gagcgcctaa tgtataagtt gcacctgcta
    31741 ggaggccacc agtaagcatc agaccaatac cggtaatcaa aaaatgtttc aatctcaatt
    31801 tatgtgtcct ccttcaaacg cccgctgaat gcagatctct ttttgtaata aaacgtcgtc
    31861 ccatccattt aacaaagttc atcatgacat aaaaaagccc ttttgttcct ttcagcaaca
    31921 gtggcgaaat aaatactgcg cttcctgtgg cgaacagacc gacaccgata aagaataatc
    31981 cactcagaat cgattgtcca aacacactga ttcctgagat gatcatgaat aggccaacac
    32041 ctaagatact gatacataaa acatagatac taaaaatgag cgctagtaaa gcaaccaata
    32101 gtcccccaat aaccaatata aaccccagtg ctagtggaat gaggacagga gaagcaaata
    32161 aaccgataat gattaaccaa atcaggcgaa tttgtctttt tggatttttc actaactcgt
    32221 tttctgcttg ttcttcttct gaaaagtaac tgatttttaa ttttaaggca aactgctttg
    32281 gtgtaccata ttcatcaatg atctgatcta atgtttttcc agaatctaaa aaatattcct
    32341 cataatactg gatcaaatca aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa
    32401 cttcattcaa atattcattc atttgtgctc tcctccaata accggtctaa agaatctcga
    32461 aaaacaatcc attcatcaat aatttcttct aaatgacgac ttcccattgg tgtcaacgag
    32521 taatatcgac ggtttctgcc ttcaaaaggt tcatcatagg tctcaagata tccttctttt
    32581 ttcaatcttc tcaaaaccgg atatagtgta gattcactga cagagagact ttcttgtacg
    32641 atttttgtta aggcatcccc atacatatct tccttgctta atgctgctag cacagtacca
    32701 tcaagaagaa cagttggaat ctggatgtgc attacgtcac cttctattct caagtgtaat
    32761 attttatttc aatctatatt atacaacata taatatagtt tcaaaatcag tttgtcaaat
    32821 acaaaaaaac tgactaaaag atttgatcct ttttagtcag caaaaaatgg cttctctttg
    32881 atttttatta taacagtggc ataatccaat ttgtccacat ccagctcagt ggcatgataa
    32941 cgatcattgc aggtatcatg tcagctactg ggaacatttt cacgttgatc attctaaaac
    33001 cggtagcgac catcaagaat ccgccgcaag ctttaaaatc taagatcatg tctggtgtag
    33061 tcaatggata aataaatttt gccaataaga acaaaatata aaaaataatg aattggggaa
    33121 ttgctatcac agaaacgaca taccctaaat tacacgcaaa aatagctgct gtgaaaaaat
    33181 caaggacaga tttagaaatc aaaatcgtac tgtctcctgt catcccagaa tctaaacttc
    33241 catagatccc cgtaccactt gcacaaaata gaacaatgac cgtcactaat gtgctgataa
    33301 attcatcgtg ggtcatacca agcttttcac tagggaagat tttagaaata ggtacttgca
    33361 tcagcgcagc ccccttattt atcccattac ctaaatgaac aatcaaacca atcgctgttc
    33421 ctatcactaa agcaaaaata acagccggca tatttttcat cggcgcaatg gaatagattc
    33481 ccatccccat cgaacacaca ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta
    33541 atttatttcc tactaatccg ccaattagtc cgccaaacaa aacggaacct acattgatca
    33601 aaactccagt tggcatcaaa aaactcctct ctttttctac gaagcgaaaa aaatcagaaa
    33661 aatttctgta attattgctt tcgctgcaag aaagattgta acagacagag aagattacac
    33721 caattcattt tttgtgttaa agtattcttt attggaataa gtaggaggaa tatgatggat
    33781 atccataaat tcgaagtttt tcttgattta gccgaaacaa tgaatttcac caaaactgct
    33841 gatcgacaat tcaccaccca aggaaatatt tctaaacaga ttctttcgtt agaaacagaa
    33901 ttagacgtca aattattcga acgcgccaat agaaaaatcg aattgaccga agctggaagc
    33961 ttattgctcc catatgtaaa aaatgtcgta gaacaatacc atgctatgca agaagtgctt
    34021 gaaacctata cgaaagacaa caatctttca ttaaatatct taacgatacc gacaatgatc
    34081 aattataaag gtttttccaa aatcaccgag tttttgaaac gccatcctga atttacagtc
    34141 cagttgaaag aagtggaaag tgtcgaatta acttacaatg acgccaatga acatagtgac
    34201 acgatccact ttgctagaag tttccaggcc ccaccaagtt cgattgagtg gttgcctact
    34261 gaaacagacg attttgtagc agttctacct aaaaatcatc ctttagctag taaaaagaaa
    34321 ttggatctaa gcgaactgaa aaaggagaac tttttgctcc ttggccccac tacgaacttg
    34381 tatcaacctg tactagatct gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa
    34441 ggagcaagaa tcgacttgat catcaatatg attgcaaatg acatggggat tgctattgtt
    34501 atggaaaaaa cagtcaagaa tctattaaaa gaaaatactg tgattctccc tatttctcca
    34561 acaacagaga gttatcttgc atttactcgt aaagtcggtg agcattcttt ggcttcagat
    34621 acattttggg agtatttaaa tcaagaagaa aactaagccc tacattttct gtccatctat
    34681 ccacccaaaa aaagagagcg acgcatgact ttcgctctct ttcttgcatc atgtccttgt
    34741 ttcaatgaca cctctaacat ttctcgtagt tcaataagaa agcagcgatt tttatgccaa
    34801 aagaaccgta agaacatttc cctaatcaca aaaaaacgac taaaaacccc taaaaacctt
    34861 caatagaatt tttaggggtt ttattctttt tcctattgtt tacttaaccg tatcattgct
    34921 gtcgttccta ctccttcgat actgctataa tcaatacgtc catcatgcag ttctagaatt
    34981 tttttaacca gtgaaagtcc tagcccattc ccagaaattt gacgacttgt atctccttga
    35041 taaaattttt caaacattcg atctctggtc tcttcattca ttcctacacc tgaatcggtc
    35101 acttccagta caatctctgt ggctgtctca aatagtttga tatgaatctg tccattgatt
    35161 tgattgtact ttatggcgtt atccatgata ttcagccaca cttggtacaa aaactcttca
    35221 ttgcctgtgt aatttactgc agcaagttcg atatccaatt ctagctgttc tttctcccat
    35281 ttaggctgaa aaaaaacgat tacttctctg atttgttcat ctaatcgata ctctttcttt
    35341 tccagttgga tacgctggtt ttccagtttg ttcaatttta agatgttctc tgttaattgt
    35401 gaaagctgcg tgatactttc gataatccga tataaaaata tttggcgttc ttcgtcagag
    35461 agatttgggg cttgtaacaa ttgaacatat ccttggattg ttgctagtgg tgttttgaat
    35521 tcatgggata cactggacac aaagtcattc ctcagtgtct cgatactatt gagttcttgg
    35581 accataacgt tgaaatcttt atacagttgc tgtacttctg ctactttctg ctgttcatcc
    35641 attcgaatcg aaaaatctcc cgttgctacc agactcatat ttgtccctag cgcactgatt
    35701 ggatgcagga tacgccgccc aacaaaaatc gatattcctg tacccacgac catactgaac
    35761 cccgcaaaga cgaataacgg gtataacgtt ggttttccag ccgattcagc aggcgagata
    35821 gaatgtcgag acaataagaa aataaaaccg agaaaacaca gcagtgtcac taagatcgaa
    35881 atgaagacaa tactagcaaa atagatccat aattgtgaaa atagcttttt tttcatcttt
    35941 aaataaccgc cttatagcct agtccccgta ctgtgatgat ttgaaaatct tccgtctttt
    36001 caaaacgggt ccttaaacgt ttgatatgta catcgatcgt tcgttcttct gtattcgtat
    36061 ctaatcccca aatatcatcc atcaactgtt ggcgagtgaa gatcttgttg ggataagaca
    36121 gtaatttata cagaagcaga aattcttttt gaggcagaat caacgaatgc tgatcttgta
    36181 acactgtata agcatctagc tctaattttg tttgtccgat tatcaagcta ctgctgtaat
    36241 tgatctttga acgtcgaagc aatgcttcca cacgtaggat catctcattg acatcgatgg
    36301 gttttgtcat ataatcatct gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg
    36361 cctttgccgt aatcattaga acaggtgtgt caaatctggt gctacgtaat gagtctagca
    36421 agtcatagcc atccattttc ggcatcatga tatctgtaat gattaattct atacgtgtcg
    36481 cttctagttt tttcagcgca tcctctccat tcaatgcggt gatgactgaa aagcccgctc
    36541 gttccaacac agaacggtaa agcaactgta attgcggatc atcttctact actaaaatac
    36601 gattcattga tctccctctg ttttttgaaa aatttctaga ttttttcttg tagttcatct
    36661 tcagttaaca tttatttcgt atactacgat cattgttctg atatacttat tagtataccg
    36721 aatatttcaa tacgggagaa gggtgattat gcttcaatta aaagatataa aaaaatatta
    36781 taaagtcggc gaaaccacca ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa
    36841 agaattcgta gcgattttag gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat
    36901 tggtggacta gataattatg attccggtga tatggtaatc aacggaaagt ctactaaaga
    36961 ctttaaggac agtgactggg atgcctaccg aaacaactct atcggtttca tttttcaaag
    37021 ttacaacttg attggtcatc taggcattat tgaaaacgtt gaacttggga tgacattaag
    37081 tggtgtttct aaagacgaaa aacgaaaacg ggcagaagat gcattgcacc gagtcggctt
    37141 gacaaatcat atgcataaaa agccaaatca gttatctggt ggacaaatgc agcgagtggc
    37201 catcgctcga gcactagcca atgacccaga tatccttttg tgcgatgaac caaccggagc
    37261 gttagataca gaaacaagta tccagatcat ggaattgatc caagagttat cgaaagaaaa
    37321 attagtgatc atggtcacac ataatcctga actggcccat caatacgcag atcgaatcat
    37381 tgaattttct gatggtaaga tcttaacaga ttcccatcct cacatcgaac gaccaaaaga
    37441 tgatcaattt aacttacgca gaacaaagat gagtttttgg acagcgttaa agctttcctt
    37501 caacaatatc cgaacaaaaa aaggacgtac tttcttgacc tcctttgctt ccagtattgg
    37561 gatcatcgga atcgcgatcg ttctgtccct ttcttctgga ttccaaaaac aaatcgataa
    37621 cacgcaagct gaaacgatgg cgaaatttcc gattactatt tctaaggtaa cgactaacca
    37681 gactcgtgac gatgccggat tgggtgcaag caaagcggat tatccagaca gcaaaacgat
    37741 tactgcaaaa gtcagcgaag aagataaagc acagcacacg aataagattg atcaaacata
    37801 cgtcgattac gtaacagaca tcgatcctaa cttaagtaat aatatcggat ttacccgtac
    37861 tacaggaatc aatcttttac gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa
    37921 tcaaaatcct gatacggaat ctctttcttt atctagtacg atgtcagcaa tgacaggtgt
    37981 gggagtgtca agtttcccta cacagcttga cactagcaaa gaaaactttt taaaagacaa
    38041 ttattcttta cttgccggaa gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga
    38101 cggcaataat aatactaata tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga
    38161 cgaaaaactt gattttgatg atatcgttgg tacaacattc aaattagtaa acaacaatac
    38221 ttactataca aaacttccaa ctgggaattt tatccctaat acagattatg atgcgatgta
    38281 tcagaatgct tctgatgaat tgaagatctc ggggatccta cgcgtgaaat cttcttctac
    38341 aatgaatctc ttatctccag gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa
    38401 cgaaaacaaa gaatcagaaa tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac
    38461 tactgaaaaa gttgacgaaa atgccaaaca aacattgctt agttatttag gtggagacag
    38521 cttgccttca agtatcatga tttatccgaa caattttgaa gacaaagaaa agatcttgga
    38581 ttatctagat gattacaata aaggcaaatc agatgaagat aaaatcattt atactgattt
    38641 agctggaacg atgactgaac tgactggcgg attgatggat gctatcactt atgtccttat
    38701 tgcttttgca gggatctctt tagtaacaag tatgatcatg atcagtatca ttacttatac
    38761 atctgtgatt gaacggacaa aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa
    38821 agacatcact cgcgtatttg acgcagaaac gtgtatttta ggtatttctt ctgggatatt
    38881 aggtgtgttt attgcatggc tagctacgtt ccctatcaat agcatcttat acaacatgac
    38941 ggatctaaaa aatgttgctc aactaaatcc tgtccatgcg attattctag tgatcgtctc
    39001 tactatattg acgatgcttg gcggtcatct tcctgcacga atggcagcga aaaaagacgc
    39061 cgctatcgct ttgcgtgcag aataaggttg tgacaaccac atttagattt gagtcataag
    39121 agcgctgaaa tagtagaaaa cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc
    39181 tgtttttttt aattattttt tataattctg gtatactttt ctttcctttg taccacaact
    39241 cttctggcgt atacctgtct tttccattta tgaatactgt tccatctgct tcaatcgtta
    39301 cttcagtccc aatccacata ttacgagaaa gtgcgagaag attttttctt tttcctcttc
    39361 ctttttttcc aaattgcaag gcatcggtta tctcttctgt ccaatgagat ttcacgtatt
    39421 ttacttctcc gttttcaaat gtaactttca aaatattcgc tatattttcc acttttactg
    39481 ctttaggaaa tgactcctcc atttttttca ctttccttcc tccaacacta agtatgcgct
    39541 ttcttttagt gataagtata tcatagttta ttctattatc aaaaaaacgc atccattcca
    39601 ttgtagaata ctttaccgaa caagttgaat tgttttcctc tttttcacct tttactatag
    39661 ataggtaaag acaactaagg aggaatctct catgacagat caaccgtatg atttaagaaa
    39721 agtcatcgaa gaactaaaaa cacttccagg tcaatatcac gaaacagata ttgaaatcga
    39781 tcctgaagca gaattatctg gtgtttaccg ttatatcggt gctggcggta cagtaaaacg
    39841 tccgactcaa gaaggaccaa ctatgacttt caacaatatc aaaggtttcc ccggcacacg
    39901 tgtgaacatc ggaacaatgg ctagtcgtaa acgtgtaggt gaaattttgc accatgatta
    39961 tcgaacactt ggtcgtttct tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt
    40021 agacaaagca gacgctccag cacaagaagt cgttcatcta gctaccgatg cagacttcga
    40081 tattcgcaac attttgcctg cacctacaaa tactcctgaa gatgctggtc catacatcac
    40141 aatgggtgta gtcttaggat cagatccaga aaaaacaatg agcgatgtaa cgatccatcg
    40201 tatggttcta gaagataaag atacgatcgg tatgtacatc atgcctggcg gacgccacat
    40261 tggtcacttc caaaaacaat tcgaagcctt gaataaacca atgcctatca ctatcaatat
    40321 tggtttagat ccagcaattg ccattggcac gactttcgaa ccacctacca ctccacttgg
    40381 ctacaatgaa ctatggtgtg caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg
    40441 tatagcagtc gatgaagtgg gtattgctcg tgctgaattc atcattgaag cagaaatcat
    40501 gccaaatgaa acaatgcaag aagatatcaa cacgaactca ggaaaagcaa tgccagaatt
    40561 tcctggctat actggggatg ctaatccagc tgtacaagta gtgaaagtca aagcagttac
    40621 tcaccgaaaa gacaatccaa tcatgcaaac aacgattggt cccagtgaag aacacgtatc
    40681 tatggccggt attcctacag aagcaagtat tcttgccttg gtcgataaag ctattccagg
    40741 aaaagtagta aatgtctaca atcctcctgc aggtggtggt aaattgatga ccatcatgca
    40801 aatccgtaaa gaaaacgaag cggatgaagg tattcagcgt caagcagcta ttcttgcttt
    40861 atcagccttc aaagaattga agacagtcat tttagtcgat gaagatgttg atattttcga
    40921 catgaacgat gtagtttgga ctttcaacac tcgtttccaa ggagataaag atatcatcgt
    40981 gatcccagga atgaggaacc atccattgga cccatcagaa ttaccagaat ataatcctgg
    41041 ctctattcgt ttccgtggga taagtgcaaa aactatcttt gacggtacag ttcctttcga
    41101 tatgaaagaa cattttgtac gtgcaaaatt ccaagaagta gaagattggg aaaaatattt
    41161 gaaataatga atgatctgca actgttttga catcataaaa aggagtgcgg aaaatggaac
    41221 aattggcaca acggaagaaa aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt
    41281 atgccatcaa cctagtgaag aaactaaaag aatatccgat gatagaaaca cacggaatca
    41341 tcagtccatg ggcaaaacag aacttaaagc tggaatccga cttatcacta actgaattcg
    41401 aaagcctgtt tgattaccat tattcaaaca aagatttagg tgctgctgtc gccagcggat
    41461 catttttgac cgatggaatg atcatcgtac cggcaagtat gaaaacagtc gcaggtatct
    41521 ctgttggttt aggcgacaac ctgattgcac gtgctgctga tgtaacaatg aaggagcaac
    41581 gaaagttagt cattgttcct cgtgaaacgc cattaaatgc cattcacttg gaaaacttga
    41641 ctaaattagc acgactaggt gtgcaaatca taccaccaat tcctgctttt tataatcagc
    41701 ctcaaacgat ccaagatctt gttaatcacc atacaatgaa acttttggat caatttgaaa
    41761 ttgtaaatga gcattcaaaa cgttggcagg gtgattaata tggaaaaaga aacgatagtt
    41821 gaagtaacca aactaggttt cacgatccaa gctgctgtta aagtaatcgg taaagactta
    41881 ttgatcactt taacaggcgg agatacacca catatcggaa cagtcactac attttctcgt
    41941 gatacagaga tgcaaactgt acgtttccct agccacgatg gtcggttcca taaagacgat
    42001 gtcttagccg aaaaaatagc tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc
    42061 acttccggta tccatgttga ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg
    42121 tcagaagagt taggttacaa gattttagat tggttggaaa aaatagattt ccatatcaaa
    42181 gaaccagttt attataaaga tggtgaaaaa ccattatgaa agaagaggtt gtgaaatcag
    42241 cgttatgacc tgagtatgag agaaaaaaat ggaaaaatag cttctcatat tttgccgttt
    42301 ttgagctaat accgagggac agctgattga acgccgttta tcaagaggtt gtgataaagc
    42361 cgcttcgttt cgagtagtaa gaaaaatttt gtaaacatgg gtttccacat ttttgcataa
    42421 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcaagagg ttgtgacacg
    42481 tcttttgtca caacctcttt tatttttgct tcactgtaac tgctacaata tcaccgattt
    42541 cttttccgat gtttttacga atatcttttc gtagtccaag gatgtagcag actgaaccat
    42601 ctggattttt cacgcccata ttcacgatac tgccttcata tggcacattg tcaaaagttg
    42661 cttggacttt aactcttcca gcaccgaatt cttttcttat atcatacgga aaaataatat
    42721 atgccccgcc cttgccgact tcggaagcat agatttttga ttcaaaagaa taaatttccc
    42781 tcattttatt tctcctattt tttacatcta gtatagcata gcaaaaaaaa gaaggaactg
    42841 tgacaaaagt cttgtcgcag ttcctttttt cgaataaacg gtgttctcct gccaactcct
    42901 cggtcacaag tcacaatagt caacaaacat gagaagctgt tttttgaaat tgctccttgt
    42961 gactcagagt taaacggcag gctcacaacc tcttccttaa acggtgttca aaatattatt
    43021 cgccttttgc ttctttcact aattttgcga atgcttgttg ttggaattca gcagctaagg
    43081 ctttcgtacg ttgttcttgc tctgctttgc tcatttttgg attttcagtc attgctttta
    43141 ccataccgtc ttgaacataa gcttttgctt cactttccat cgtactttgc tgtttcttca
    43201 ctaattcagc taacttggct gcttggtccg tacttaattc tacccatgtc tctgggattt
    43261 tgaatgtatt cactcggctg tcatattcgt gattgttatc agcaatattg aaccagaatt
    43321 tatacatgtc gtttttgtat tcccaataag tagttttcgt cactttttca gcatcgccgt
    43381 ctgtcttttc tactttattt gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt
    43441 tttcatttgt tcggtaaaga tacacttttt cttttccatt ccctaaagct ttatacaaaa
    43501 gcatatctgc tccttgggaa tcagcactgg aaacaatttt ttctgttttt gtttccgtta
    43561 ctttttccat tccaaagtga ttggacaaat tagcgacgat caaacttagt gaagcgacga
    43621 agattagtcc gaaaatcaaa gataaaaagg tttgccatgt ttttttagcg aacacgttcg
    43681 ttaaagcaaa agcaagcaag ctgacaatca aaactccaat aatcatgcat tatcccccgc
    43741 tttcattaga acaggttctt ttttcttcaa taaaaaggca ataataaagc caagaatccc
    43801 aaagatgatc gctacgataa acgcggcatg gtaacctgac agtgtggcat tgattgcctg
    43861 atctttataa gctaatggcg tattatccaa tacagatttg cttgggatat ttgcttttgt
    43921 cacattcgtc aacacactga tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa
    43981 tgtattattt accgctgtcc catgactgat caagttattt ggcaatgcat tcataccaga
    44041 agtcgttacc ggcatcatga ccatggagat cccaaacata cggatcgcat aaaggatcac
    44101 gatatataaa actggagtcg tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa
    44161 aatgaacatc cctgtgatgg ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt
    44221 cacaggcatc atagccccca tgatcaatgc acccggcaac aacatcaagc cagaatggaa
    44281 tgctgattct ccacgaatat tttgaatata caatggtacg accatttctg cccctaccat
    44341 cgccatattt gttacaccag ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac
    44401 acgaagttcc aagaatggat gttccaaatg taattgacgc catgagaaca aggcgatgaa
    44461 aatcacacca acaactaaga aaccaattac tttcaagctt ccccagccgt catctcctac
    44521 actagaaaat ccgtaaagta aactaccgaa tccaatcgtt gataaaatag cggataaaat
    44581 atcaatgcta ggatttgaca atggaataac acttttcatt aggaagaatg ctaaaaccaa
    44641 gacaagaaca acgattggca gtaccatacc aaacaagtca cgccaatggt agttatcaat
    44701 gatataacca gataatgtag ggcctaaagc aggagcaagt ccaataacga tccctgacat
    44761 ccccattgct gatcctcttt tttctggcgg gaaaatagat agcatgatat tttgcaataa
    44821 tggcatcgat actcccacac cgcaagcttg gatcaaacga ccgattagta atgtactaaa
    44881 attaggtgct gtaaagcaag taatcgttcc gatcaaaaag atcgtcatgg cagaaagata
    44941 taaatttctt gaactgaatt tattgatcag ccatgctgtt attgggatca tgatcccgtt
    45001 gactagcaag aaaccagtcg ttaaccattg cacatcagac gctgaaatat cgaaagcttt
    45061 catcaatgta ggaaaagctg tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc
    45121 aattaataag acaacaacta aaagtgtccg attatatggt tttccgtaaa tatctacggg
    45181 ttggttgtta ctcatatttc tccttctttc tgaatttttt ctgaaactac tagaatactt
    45241 tagtcctaaa ttcgctattt gtcaacctac ttgacacata agttaatacg gatataatta
    45301 agggaagcaa gaggacaaaa ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt
    45361 tggaaaatga tcaattggtt gttggcatca gtgaattaag tgatatcgta ggtgtgtctc
    45421 cacgtcaatt aaggtattgg gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga
    45481 attgtcctag aaaatatcgt ttacctacag tcataaaagt agaactgatc aaaaaatatt
    45541 tagatgacgg atttactttg tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa
    45601 tgcaccatgt ccgtaaagtt ttttcaaaag caatcaaaga tatcgaattg atcgacgaac
    45661 gctatacgat tttacaaatc ggtaacttca aagaagagaa tgctaagatg tacatcattc
    45721 atgacaacga gacagacgaa ttaacttaca agctccttcc tgtcgatgaa aaagctgact
    45781 acgaacagtt gttaaaagaa ctctaaacgc cactaatctc ctaagtacca agctttttaa
    45841 taagtagatg ctacataaat acttgaaaaa aggcctgttt gaacataaga atatccgaac
    45901 ggtgatctgc gtttctttca tatagaaagg aattctccat cactgttcgg atattttttt
    45961 atttttttcg gttactttct tctaatacat ctgttagcca atttgcatat tctgtttccc
    46021 gattgatcgc atgcgctaaa atcaggtagt gtccgtaatt ttcatgtttt tctttttcag
    46081 taggaaagat cgtttttttt cgctctaata gatgaagtcg tttttcttca tgaaggtcac
    46141 gctgctgctg aataatttct actaatgccg gatcattcac gtctttgacg aaatacaatt
    46201 tcagaacaaa ttcgtctcga ttaaccggta gttcattggt gggtgtatga atccactctg
    46261 ccaattgttc cttcccttct tctgttaatt cataaacttt cttttccaat ttttcacctg
    46321 tgatctctac ttgatgctga atgattcctt gctcttccat ttttgcaagt tcaggataaa
    46381 tctgactatg ttttgcttgc caaaattctc ctatttcatt tttgaacgat tgattcaatt
    46441 catatcctgt catcttcttc ttacttaata accctaacaa aatatatcga agtgtatttt
    46501 ttcttgccat cagttttccc tccttgacaa atctttaaat ccattctatc ataaatgagt
    46561 aatcgaaaac atgtagttta ttacatgttt taatttataa ggagatgctt aaacatgaaa
    46621 gaatttaaag aattggaaga ttttattgga actcacttta tctatacgta tgacaatggt
    46681 tgggaatatg aatggtatgc caaaaatgac catactgttg attaccgtat ccatggcgga
    46741 atggtcaaag gccgttgggt aaaagaccaa gaagctgata tcgttaaatt gacggacggc
    46801 gtattcaaaa tcacttggac agagccaaca gggacagacg tggcattgga ctttatgcca
    46861 aatgaaaaga aacttcacgg aatgatcttt ttccctaaat gggttgaaga acatccagaa
    46921 atcactgtta ctttccaaaa cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat
    46981 gaaacttatc caaaattact tgttcctgaa tttgctacga tcacttatat gggtgatgca
    47041 ggtatagaca atgaagatgt tattagcgaa gaaccatacg atggaatgcc agatgatatc
    47101 cgtagcggaa aatacttcga tgaaaattat aaacgagtaa aataaggttg tgaaagagag
    47161 gttgtgaaat cagcgttctg acctgagtat tagagaaaga aacggaaaaa tagttcctca
    47221 tgttttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcaaga
    47281 ggttgtgaaa tcagcgttat gctttgagta ataagaacgc tagttagaaa atagcttttc
    47341 atattttcta ggctagtggg cttattaccg agaagcagcc gtttgaacac cgtttatcaa
    47401 gaggttgtga aatcagcgtt ttgacctgag tattagagaa acaaatggaa aaatagttcc
    47461 tcatattttg ccgttttttg agctaatacc gagggtcagc tgattgaaca ccgtttatca
    47521 aagattgtaa caactgcata ttcacacagt taaattagtg acttcaaaat gaaataatgc
    47581 tcccagcaaa aaattaggct gggagcatta tttcgttatt ctgttcatca aaaatcagcg
    47641 ttttgattcc atttcatcac aagttcctcc tcatttactg cttcatcttt ttgctgtgtt
    47701 tgaattgtaa aaccttgtga aagatagaaa tgaatagctg attgattttt ttgatagact
    47761 gtcaaagtca gtgttttatt ggtttctttt atttttttca ttagttgcgt accgattctt
    47821 tgttgccggt attccctttt cacaaagatt cctgcgataa actgatcaat caccccagca
    47881 aaaccaataa tttcttgatc tatttctgct aagtaaatca ctgcatctgg taaagctttc
    47941 ctaacagatt ctacttgatt ctcccaataa gttgatggaa tgaattcatg agaatccaag
    48001 ttagcatgca gccaaatcga tagaacagat tccagttctt tggcagacaa ttttgttgtt
    48061 gtgcggatca ttttttctct ccttacttat tcattgattt taaaataaaa accatttttt
    48121 tgaagctctt ttacaagttt tcgcccctca aaaaagtata tcttctcttg ttttactttt
    48181 ctaataccaa aagaaacagc aactttagga tcatcagctg aaacgattcg ataagttcca
    48241 ccaggcaatt gatccagctt gactttccca tgatggtcgc tttgtactga aaacggcaca
    48301 ccatttctag taagtgtttt tttcttatgt ttcaaaatga atgaactacc atttaccggt
    48361 ttccctgacg aatcactaat aataaatgaa aaatcaagca cttttttact taatctatac
    48421 tgatagatta gtcgtccgat aaacactaac agtatgacta cagctattat cgttcctgca
    48481 agaataaacc attgccgcat ggttctttcc tgatttcctt ttccttcttc tttttttacc
    48541 gattcgttat aaggaatccg atgtcctgtc actagcaaac gatgcgtgtt gatcatatac
    48601 ggcgtacatg taagcaaggt aactaagtct tctccttctg aaatcgttag aacagacgtg
    48661 tcatctggca gtaccacttg tatattttta acttgataag ccaatttctc ccccaaaaca
    48721 gtcaaaataa acgtgtctcc gttttctaaa cgatctaatt cggtaaataa ccgtcgatta
    48781 ggtaaaccgc tgtgagcaga gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag
    48841 gttccttgca agacgcctgc tccattttca aggatcctac cattgagccg atcataaagt
    48901 gaaatattta attggatctt aggtatattg atacttccga tcagccattc ctctaattgg
    48961 ctggatgttg tactttcatt tctgttcttt tcattgaaag gatcagtacc aggattgaat
    49021 ccttggtcag ttaatcgttg gtttttctcc tccattttct ttcgctgcac ttcaatttca
    49081 gctgcggtcc gtttttgtac ttgttcgata cgtttttgat caataaaact atttaaagaa
    49141 tccacataaa atggatataa agcgatcagt aaccctacga cgatacatat taataaaaac
    49201 aaacgaaaca aaagtcgacg agactgtttt tgcttactct gcttcacatg tgtcctccct
    49261 tgcaagtttt tttgttcttt cttttattat atgggttcag gaaaaaaata gaaaatgaaa
    49321 gccgatgagg ttgtgaaaaa gctgtcctgc atcaagtaat aagtacagcc caaaaaaata
    49381 gctactcata ttttttgttg ggttgggctt attaccgcag atgcaagctt ttgaacaccg
    49441 tttaaggaag aggttgtgaa aactgcgtta tgctttgagt aataagagcg ctagtcagaa
    49501 aatagcttct catattttct aggctagtga gcttattacc gagaagcagc tgattgaaca
    49561 ccgtttaccg agaggtcatg aaaaagctac tttgtttcga gtattaagaa aaattctgtt
    49621 tcaaaacaag gagatgcaac aaaacttatg tcgcatcctc tctccttaga actttcactt
    49681 atgtttttct tgtaaaattc ataaatattt tcatgaattt tctaatttct tgcctacctc
    49741 tcgcatagga tatatgatga atttatctaa agtttgcaaa ggagttcgac aattgaaaaa
    49801 atatggccca tttttagcaa tttttttcat tgctttggca tttctcatgc ctcatttatt
    49861 cacacataaa ctaattttgg gtgctgatgc cccattccat tacaatcggt tctatgatgc
    49921 cgcgcaacaa attaaatatg gggatttcca ttattttcct tccctttatg gattccagca
    49981 aagtggcagg atcgtgaatg ccgtttacgg cccatttttc gcttatttac aaggattgat
    50041 cgtgttgctt gcaggtagct ggtttcatta tcagattctt tcaaatttca ttctttatct
    50101 tatttcagga ttttctatgc tgattttact gagatatgtc aatgttcgaa attgggtaag
    50161 tgtaccagta gccgttttat tcatgagtac gtattctatt caatactggt ctatcaacca
    50221 aggattcact agctggggga cttgttttta tcctttatgt atgatcccta ttgctgattt
    50281 cgtccttcat aaaaagttcc ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca
    50341 gatccattta ctgtcttcga tcatgttagc tttgatgtat atcccttttt atctttatta
    50401 cttttttaac cagactgaaa aaggaaaagc actgcttcaa ctgattaagt cggtcttgct
    50461 ttattttgtt ctgacagcaa atatttgggc aggattagct attgtttaca gtgggaataa
    50521 gattcttcct ccctttgtta acagagaaat gagtcaaaag gcgatagatc tggatggcag
    50581 ttattggctc cattacccta aggcctttcc tatattgata ttatcaggtt ttttgctttt
    50641 tattttcttt caacaaaaga acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt
    50701 tttctttttt ctttctacta gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc
    50761 ttttattagt ttgatccagt ttccatttcg atttttcgtg ccatttacgc tattgcttct
    50821 actttacctt gccctttcac tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag
    50881 tatatttggc attcttattt tttcagcaca gacgatccag aacttatacc agcatctgga
    50941 aaagtgggaa aatgaaacat ttgtcagtcg acatacttat ctattcgata cgccggaaga
    51001 agcaaggaaa acattcttca gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac
    51061 cacacctgac tatctgccaa tctataagga gacaaaagac aataaatacg accggtataa
    51121 cgaagaaata ctagaaaacg aaacaacttt tataaaaaca catccaggcg gtcttttgac
    51181 aatcaagtgg aaaaacactg gggacaaaac tgttcatatc cctgttatcg tctacgatcg
    51241 gactgtgcta cagcaaaatg gaaaaacatt gactgattat gaagtaacag atatcgggac
    51301 cccaatcgtg aagcagcaaa aaggaatcaa cgagctcacg cttcattatc aaacgccaat
    51361 ctatttctat ttcattcttt ctcttacttt gattggctgg tttaccttgc tatgtttatt
    51421 tatttatcat agatacaagc ttttacgtgc ttaggcaaaa gggataaata catccgatca
    51481 aaaaactctc caaacggtat aatcgtttgg agagttttat tttaatcagt ttttttatgt
    51541 tatatgttat attttttatt cgttcacatc taattcgatt tccaccccgt agtgatcact
    51601 gatgattgga ctatcgatcc catcaaaaat ccgttgatat tttttaacct gcattccttc
    51661 agggacgaaa ataaaatcta tacgcaattt ttctgtattt tgttcccaac catctatttt
    51721 cttttccaca gtagctgctt ttcctttttc attcgcgaca acatacgaat cttgaagaag
    51781 aaggcaactt tctcttacag tataataacc ttgagagtct actggattat tgaaatcacc
    51841 taggaaaaaa agtggtacgc gagtttccaa aaagtagttt tccaagtttt tccactcaaa
    51901 ataaaagcca ctttttttat ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc
    51961 ctgatcatct atctttgttt tcccaatcaa gatcttgcgt gtctgataat cttctttctt
    52021 ttgaaattgg gagacggtaa ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt
    52081 tccttcttca tatttatcat aaccgatgtg gctcatttcc caactccaaa aatactcttt
    52141 tccgtgttcc tttaaaagct gaactaaacg gtaggcaaaa ttatctttac ggataggtac
    52201 ttgattcttt accggacaga acttcattag cttcgcaggt tctacttctt cagattccag
    52261 taattggttg atttcttgta aagcaatcac ttcataatct tctaacagga tcttatctgc
    52321 taattcttgc aattttttct ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat
    52381 gaatttcatt ggttttctcc ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt
    52441 tgtcacaacc tctccagcaa atagtgttct cctgctaact cctcagtaat aagctcaatg
    52501 gaccaggagc tattttttgt ttggctgttt ttatcacttg gtgcagggca gctttttcac
    52561 gacctctcca ctaaacagta ttcaaactgc tgcttctcgg taataagccc aaccaaacga
    52621 aaaataggag gagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    52681 cacaacctct ccttacattt taatggagcc gatcacttta ccagctgtca ctgtacctaa
    52741 ttgcttgatc actacttctt ctactttatc gtttgtaaat acgatcatga tcgttggatc
    52801 tttttcttcg ttttggatca catcaagatc catctcagct aatttcgttc ctgttcctac
    52861 ttcttggcct tctttgacag caatctcaaa agctggctga ttcatttgga ctgtatcgat
    52921 tcccatatgg atcaaaactt cgataccatc tttcgtttcc agcccaattg catgtttgct
    52981 agggaaaata cttgtcactt ttccagcgat cggcgcaaca acttctcttg atgttggaat
    53041 aacagcaaag ccgtcaccca tcattttttg tgagaatacc ggatctttta cttcagataa
    53101 aggaatcact tctccagaag ctgcggaaac tagttcttgt tctataccta agtattgctt
    53161 actacctgct tgtccatttc ctgtagaaac catttctggc tctggaatct ctgctcctga
    53221 ttgcagcaga tcttcgatat ctgatttcaa gacatctgct tttggtccat aaactgcttg
    53281 gactccattg tctttgacga tcaatcccat cgcccctgct cgtttccagg cttcttcagt
    53341 ccctactttt tctcgatcat ttacacttac gcgtaaccga gtcatacaag catcgacatc
    53401 tttgatattt tcttttcctc ccagcaaatg aatgatctgg accacttgct gatctgctgt
    53461 tcctgttccg ctgttagata caacggtttc ttcactgtca ttgtcgtaat ttccgttacg
    53521 tcccggtgta gcaaagttga attttttgat caagaaattt gctaagaaat acgtaacaac
    53581 accaaaagcg atcacacaca acacgaagtt gataagatct ccacctagac ccgctttgat
    53641 agctaacggt gtccgagtca gcaattcgat attcccaaat gaatggaccc gcaatggcaa
    53701 gatatctgcc atcgcaaatg cagccccttg gatcactgaa taaatgacat ataatggcac
    53761 agcagcaaac ataaacatga actccaatgg ttctgttaca cctgttagaa agacagccaa
    53821 cgctgctgaa aaatacattg atttatatct tgctttctta tcaggatcga cattacgata
    53881 catcgcaaaa gccattccca tcaagatacc tgaagaacca atcatttgtc ctactttgaa
    53941 acgagccggc gtccagttat gcaacacaaa ctcatattgc gacatatccc ctgcaccttt
    54001 aagattcact aaatcagtgg cccaagccaa ccataacgga tcttgcccaa atacttgtgt
    54061 ccccgcctgt gcgcctgata aaatctcata cgatccgcca agctgtgtat agttgatagg
    54121 aatcgtcagc atatgatgca atccaaatgg caatagtaaa cgttctaatg ttccaaataa
    54181 aaacggtgct agaatcggtg cgctttcttg tgattctgca atccataaac caaaattatt
    54241 gattccagcc tgtacgtttg gccacaccac agcaagaatc aatgccacga ttgttgacca
    54301 taagataaca acaaacggaa caaagcgctt tccattgaaa aatgacaaag catccggcaa
    54361 ttttctataa ttatagtact tattataagc cattgccccg acaaatccag cgatgatccc
    54421 tacgaatact cccatattca aagctggtgc ttccaataca cttgtaaaaa atcctttaac
    54481 catgatcttc gtaccgaaca gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact
    54541 tgtgacacca aagatagccc cagtgatccg attgattaaa ataaatgaga tcccagctgc
    54601 aaatgctccg cctgctcttt cttttgccca acttcctcct attgctaaag caaataaaat
    54661 atgaagatta ccaataattg cccaaccaat attttcaatt actccgcccg ttgtgacaag
    54721 cggcgtccaa tcaggattga tcaagggaat tgatttccct atactgatca tcaacccagc
    54781 tgccggcata actgctacca caaccattaa agcctttcca aatttttgcc aaaactcaaa
    54841 actaaatatc tttttcattc cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt
    54901 atagcgtgcg ttttcttttt tgtaaatgct ttatttttta tttttctgtc tataccattc
    54961 tataaaacct ataatacgtg tgttcccttc tataattttc tcctaaaaat ttattttttt
    55021 aagctttttt ctatgaaata ttttcttttg tctattgact tgtgcaatcg gttgcgttta
    55081 ttatgaacgt ataaaacagc tcattaggag gagaaaaatg aaacagatca aacgactatt
    55141 tgctattgat ccatggaaaa tcagcacgac acaattagat aaaaaaaatc ttcgactgca
    55201 agaatcttta acaagtattg gaaacggtta tatggggatg cgcgggaact ttgaagaaac
    55261 ttactcaggg gaccaccatc aaggtactta tttggcgggt gtctggtatc ccgataagac
    55321 acgtgtcggc tggtggaaaa atggttatcc agaatacttt ggaaaagtga tcaacgcctt
    55381 gaactttatc gcgatagata tttcgatcaa cgatcagctg atcgacttgg cttctttgga
    55441 accagaagac ttttactggg aattagatat gaaaaatggt gtattatctc gtacattcac
    55501 aatcactact tctactaata aagtacgcat ttccttcgaa cgttttctga gcattgtcaa
    55561 aaaagaatca gcttatatct atttaactat agaaatgctt gaaggtagcg gaacagtcaa
    55621 actcgtatca aaattggatg gaaatgttca aaacgaagac agcaactatg atgaacattt
    55681 ttggaatgag attgatcgtg gaactataga agaaatcggc tatttgacga ctaaaactat
    55741 tccaaacaac tttgggattg aacaatttac tgttactgca gcaatgcgcc atttcgttaa
    55801 tggaaaatca gtcatccctg actatcacga agatacttta gcattacatg gttgcttgtc
    55861 tgcagaatta tctcaaggtg agacatggtc agtggaaaaa gaaattatcg ttcttactag
    55921 tagagatatt ccagaagcgc agcaagcaga aaaagctgtc tcagaattaa cgaaccagac
    55981 aaacaattat atccaagcaa aagaagagca aacagcggct tgggcagaac gatggaagct
    56041 tgccgatgtg gtcatcgaag gagacgaaga agcacaacaa ggtattcgat tcaatttgtt
    56101 ccaactattc tctacttact acggcgaaga tgaacgattg aatatcggcc ccaaaggatt
    56161 cacaggagaa aaatacggcg gagccactta ctgggataca gaagcgtatg ctgtccctct
    56221 ttatcttgct ttagccaaac ctgaagtaac taagaatctg ctgaaatatc ggcataatca
    56281 actgccgcaa gcaatccaca acgctcagca acaaggatta aaaggcgcgc tttatcctat
    56341 ggtcaccttc actggtgtcg aatgccataa cgagtgggaa atcacattcg aggagattca
    56401 ccgaaatggc gcgattgcct atgcaatcta caattatacg aattacaccg gcgatacgga
    56461 atatttgaaa aatgaaggac tggaagtttt agcagaaatc gcacgcttct gggcagaccg
    56521 tgtccatttt tcaaaacgag caaataaata catgatccac ggtgtgaccg gcccaaacga
    56581 atatgagaac aatatcaata acaattggta tacaaatacg atcgcagctt gggtcttgcg
    56641 ttacacgcga gaaaattatt taacttatca aaaagaagct gctatcacta tcagcgaaga
    56701 agaactagcc aaatggaaag atatcgcaga gaacatgtac ttcccagttg acgaagaatt
    56761 aggaatcttt gtccagcatg acacattctt agataaagat ctcatgcctg tatccgatat
    56821 tcccacaagc gatcttcctc tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg
    56881 cttcatcaag caggcagatg tactacaggg gatttatttc ttcaatgata cttttacaaa
    56941 agaagaaaag caaaaaaact ttgatttcta tgaaccaatg actgtccatg agtcttccct
    57001 ttcgccaagt atccattcga ttttagcagc agaattagga atggaaagca aagctgtaga
    57061 aatgtaccag cgaacagctc gcttggattt ggataattac aataacgaca cagaagacgg
    57121 cttgcacatc acttccatga ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat
    57181 gaaaactgcc catgatcagt tgagattcgc accatttctg ccaaaaactt ggaaagctta
    57241 ttctttccat atcaattatc gcggacgttt attgttcatc tcagtcacag atgaacaagt
    57301 tactttcgag ttattagaag gttctcctct agaaatttac gtctatgaac agtcttattc
    57361 tttagcggat cgtctagaaa taacgctaag aaaggaggaa gcaaatgttt aaaggggtat
    57421 tgtttgattt agacggtgtc attaccgata ctgctgaatt ccattatcgt gcttggcgaa
    57481 agcttggtca ggaaatcggc attacgatcg accacacatt caacgaacag ctaaaaggcg
    57541 tcagccgaga agactcactc tctttgcttt tagcttatgg cggaaaagaa cactcttttt
    57601 caaaggaaga atttgcagaa ttagcgaaaa gaaaaaacga ttattattta gagatgatcc
    57661 agacgatcga accaaaagat gtcttccccg gggtcgtgcc tttactggat tctttaaaag
    57721 aagaaaaaat caagattgct cttgcttctg caagtaaaaa cgggccattc ctacttgaaa
    57781 aaatgggtct tacaccttac tttgatgcta ttgccgaccc ggcagaagca acaaacggaa
    57841 aacctgcgcc agatatcttc ctccttgcag caaaagctgt cggactaaaa gcagaagaat
    57901 gtatcgggat cgaagacgcg caagccggta tccaagccat cctctcaagt ggcgctcaac
    57961 caattggcgt gggacgtgct gaagaccttg gagaggagat cccgcttgtc cctgatacga
    58021 cttttcttac gatcgattac ttgaaagaaa agtggcatga ccatggataa tccagagatc
    58081 tccgtcgtac cagttccaga gactgaaata aagaaaatca cattgaagaa cgactactta
    58141 gaagtcgttc ttctcaatta cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa
    58201 ggaaaatcag aaaatgtttt attgtcttac gatcgtttcg aagatgttct aacagacaaa
    58261 agcttttttg gagcaactgt cggacctgta gccggaagga tccgggatgc ttcttggggt
    58321 gagcatagat tggagaaaaa ctgcggatca caccatatcc acggtggcac gaaaggctgg
    58381 tctttccaat tttgggaagt agaagttttc aaaactccat catcaatagg tgtggtcttt
    58441 tacttaaaag atgaattttc tacctatcca ggaccaatca ctgctacgat cacttaccgt
    58501 ttaacaaaaa acgaattaga gatgacaacc tctgctagta gccttgttga aaccatctgc
    58561 aatccgacca accatgccta cttcaacctt tcaggaaatg gaacaagaga catctatgaa
    58621 catcagttaa cggtcttttt agatggtgtg cttgaattag atcaagaaaa actgcctact
    58681 ggaaattgga caaaaaaaga aaaactgcca atcgacttta gaaaatcccc aacactgcaa
    58741 gagatccttg catgttatcc agatggacta gatgatgtat ttcttttgca tcatcctagg
    58801 ctttccaaaa cttccttaca attattcgag aagcattctg gtcgccaaat gactatagca
    58861 acttctaata aaagtatggt attattttct acgactggat tcgaagctga tttctctgtc
    58921 aatggaaaac agatgcattc aaactacgga ttagcaatcg aaccgcaaga aatccctgat
    58981 atcgttcatt tccccaaatt tggatcgatc aacctccatc ctggtcaaga acggatcagt
    59041 cagacgattt atcggttctc tgcacaataa caagagaaag gtgtttttat catggctatt
    59101 accgtaaaag atgtggcgaa aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc
    59161 aacgatcatc ctagtatttc cgagacaaca aagaagaaag tccgaaaaat tatggatgaa
    59221 ctaggttatg ttcctaacat gactgcccgc aatctaggaa aacgcatttc cagtgccatc
    59281 ggcgtaatct taccccctct tgattcaaaa gagcggatcg gtaacccatt ttatcttgag
    59341 atcatggaag caatcaacga agaagcgcgg aattatgata tgactacagc aatcgctact
    59401 gctaaaaatt ttgaattgct gttagagaac gtccagcgta tgcatttaca aaaacaagta
    59461 gatggtttta ttctagtgta ttcagatagt caagatactg tcattgatta tttataccaa
    59521 aaccacatcc ctttcacttt gatcggacag ccttatcatc atgagaacga aatcgtttat
    59581 gtagataatg ataaccagct tctaggtaaa caagcaacag aatttttgat ccagaatgga
    59641 cataagaata tcctgtttgt gacaaacacg actcatgaaa gtttgtattt cgaacgatat
    59701 ttcggttatc aaaaagcaat gatgatggct ggtcttcctg cgttatccag tgtagtacta
    59761 gagcatccgg aggattatct gtcctttgag aaaacgttga aagaaaacga agccacagca
    59821 ttggtggtca ttgacgatat cttcgccctt cgcacgatgc agttggcgca aatgtatggc
    59881 tatgtcattc cagagacact ctccgtcatt agcttcaaca actcgatctt ctcaacatta
    59941 acacaccctt accttacaag tatcgatatc gatatctccg aattaggacg tgtcgctatg
    60001 caaaagctgc gcgaattgat ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct
    60061 catcgcttga tcaaaagaga gacagtcgtt cctttagaaa acccatacta aatagctgac
    60121 tcactaaata cgctgaccat ttatcctatg gtcagctttt tatgtaaaag aagatactac
    60181 ttctaacaca ttgtttatct gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt
    60241 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg aaataagctg
    60301 aaattcacaa aaatttaaaa agcaattttc ttgagtttct tcttatttct tggagttaaa
    60361 cgcttctgtc ccagcctctt ttttccttcc ttcaattttt tacacgtcga cgattcaata
    60421 aaaaaccgcc taatattgta aagacaggtg caagaatcga gaaaaaggcg aatggcaagt
    60481 aatgcaacgc gctaactttc atcgttccca taatgaatgt gccgctgact ccccacggta
    60541 cgagagaatt gacggtcgca cctgaatccg ctagtgttcg tgtcaaatag gtttttgata
    60601 agccaacgtc atcaaaagaa cgcttgaatg tttctcctgg aagaatgatg gacagatact
    60661 gttctccaac gatcagattg attccgatac agctcaaggc agtaaacccg actaaacgag
    60721 aaggtcggtc catcttttct tttaattctt gaacgatggt atcaacgatc agatatttga
    60781 tcagtaaccc acctaatgcc aatgcaagta aaatcaaact agcagaacct aacatactca
    60841 tgatcccgcc tcgagaaagc agttcatcta gctttggatc aggtgtattt gcgacataac
    60901 cattcatcaa gatgtctgct gcttttccga ccgtcaaact tttctcatgg ataaaagaaa
    60961 gaacgagagc agctgtcgat cccgctagta atgtagggat agccggaatt tttttgattg
    61021 cccccccaaa taaaataact agtggaatca atgtccaagg agagatccaa aagctcgctt
    61081 tcatgttttg gatcatttca ttgatactag ctagatcggc tgaatgactg ttatgtccta
    61141 atatcgtata aaaaatcaac gaaaggacga atgctgggaa atccgtataa cgcataccag
    61201 cgatatgttc gaatagatta actcctccaa tacccgctgc caagttggtc gtatctgata
    61261 aaggcgagca gttattccct aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat
    61321 cattgaatcc aagtaaatga ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg
    61381 taaatgaact gccgacagtc actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa
    61441 agaaacgcac agaaacaagt ttaaatccat aaaccataat cgtagggatg gttccagata
    61501 aaatccatga agcaactaag acaccaatca aaagaaaaat caggattgga atgatccctg
    61561 gagtgattcc tttgatgatc ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc
    61621 cataaaacaa caaacacatg aaagctgcaa ggatcgggat atgtggcgag agctggaatc
    61681 cgatgatcag tactcctagg agtgccaaca acacgatcaa tacagcgata ctttctttta
    61741 aagaaatatt tttattcatc gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa
    61801 aaactattca cttggcagga cctcctacta aatcagcgac aaaacgtcgg ctgaaagact
    61861 tattttttta tttttcttct aaactttttt ctacctcaat cccccaagaa tctttcttga
    61921 agtgattgat gatcaatgga ataacgaaca tgaccactgt gatagctgct actaataatg
    61981 catcactcaa tgtcgctgta cctaatgtag caaatacgac aacgatgata gagaagatca
    62041 gcatgcagct gactacccaa gctaatccgt ttccgttttt accaattcgg taaggacgtt
    62101 cgaattctgg atgtttctta cgcattttaa gcaatgaaac agcaatcaaa atgtacacga
    62161 tacaataaat aacagttgta gcatttgtaa gtgtcaagaa tacagcactt acgtcatcca
    62221 ttacaccata aagcaaagca aataatgaaa taacgattga ttgtgttaga acgacatttc
    62281 tagatacgcc gtatttgttt tctttatgga atccgaattt tggtggaagg aatccttcac
    62341 gagctacttg gatgattgtt tttgatggtc cagtaaccca agctgataat tgtaaaagca
    62401 caccaataaa taccatgaag ctgaagatat ttccgatcac ttctggtaag cctaggattt
    62461 gacagtcaat caagataggc tgcgtgatat ttgctaattc catttttcca tctggtacga
    62521 catttgctac aagcatcgca ttgatgacat tcaataaaac taatccgatc aaagcgataa
    62581 atacgccttt tgtataattt tttgttgcgt ctttcaaacg aggcatgtaa acagaactca
    62641 tttcgatccc aacaaaaata aatgtgatac cagctaagta tttcaatgaa tcaatatgtt
    62701 ccaagtcagg gaaagctttt gaccatgaaa agtcacctag atagccgttt gagactagtc
    62761 cgactttgaa ggctgccaaa acacctaaaa cgaacatgat gactactggg atatataccc
    62821 ctagccatac accgatgttc ccgccgactt ttgccatatc gaatttcaag ttcaagatcg
    62881 tgatgatcca gtagataact aaaatacaac ctaaaacgaa ccagtgatta ttgccaagtt
    62941 ctacattacc aaatgtgttt cctaacaatg gtcctaaagt agaagcgacc attaccatac
    63001 ctgggaacat ttgcacccat aatagccatg caacgacaaa tccccatttg ctgcccagtg
    63061 ctgtttttac ccataactga ggaccgcctt cttgaggaag catcgtggaa agctcaccgg
    63121 aaaccattga tatcggaccg gcaaaaaaca aaactgcaaa aattgaataa aaaatcaatg
    63181 tccaaccgac tgcggctaac gtaggaatac tccgaaccgt tgcacacagt gccattgtca
    63241 gaccgataaa ggtcccaaga cccattttac ttacttcaga tttactcacc gttgacactc
    63301 tccttgattt tttggataaa ataacggtgt ctttgaagta gtttcagcaa taaacgaaac
    63361 aattcagcga atatttgagg ggattctttg agtttattgc ttgaagggtt tcttcaaaga
    63421 caacctcaaa acaaagggat cgaagagctt aggtctcccc ttgctcttca gatccaaaca
    63481 tgcgtcagaa acagttagta ttcaagctgt tttctaacat gattaccttg cttattttgc
    63541 ttcgcttgcc aataattggt ctgcgtagat tttttccaat ttttcttgaa gagctgcttt
    63601 gattttttca gcatattctt caaagtgttc ttctttgttc atgtatggtg tcattacgct
    63661 ggcacgcaat acagttactt ttccggcacg gttccattct tcatctgaga atcctagttg
    63721 gttcacaaat tgtaatgggc tgttgccata gtctgggata gcaaagtcag tatgtgaagt
    63781 taagaactca ttaccgtaaa tgcttccttt cacgtatgaa gaataatcat atacgtcatg
    63841 gttcaattta ttcattgcaa ctaagtcatc gttgcctttt tctttgaata catagtctac
    63901 catattgaaa tctggataag ttaatggatg aacttcgatt tctttgtctc ctactttaaa
    63961 tgacaagtca ttcaagaagt tgtagaaacg atgtgcacct tcgattgatg cacccattaa
    64021 tttaccatat cctgtgacat tcaaaggtaa gacatggtgt gctgcccaaa cgctggctgc
    64081 tgttgcccct gcttttgaac cttccaagat ataagcccca agtaatgctg ggatatctgc
    64141 gcctttttca aatacatatg tcgcaaagta agagataacg tctctcatgc ggatatcttt
    64201 gatgacgata ccaccggcag aatatggaac atagcccatt ttatgtgggt caatcgttac
    64261 agattctgct tcttcgattg ctttatatgc actgtgtact tcttctaaga tataatcttt
    64321 gttttctgta aagacattgt atttgtaatg aacatctttt aaatcttcaa atggaatgaa
    64381 gttgttgtct tcgtctaaga aaatcgcacg gccgtatccg ccataagcag catctacgtg
    64441 taagtagaag taaataccgt ctttttctaa tacgcgacgc aatgcaacga ttttatcgat
    64501 accgtcgatt gctccttctt ctgttgatcc tacaacaccg actacaccta ggattggtgt
    64561 tttttcagct gctagaccac gaacgatttt ttcaagttcg ttgatgtcca tgcgatagtt
    64621 gtggtcaact ggcactggaa tcacttggtc taaaccgata ccgatgatgt cggcagcttt
    64681 tagccatgaa tagtgtttag tttgtggaac caaccatttt cctaattttt ctaaatgttt
    64741 tccgctacgt gctgagtgag ctttgatttc gtcgattttt tctgggacgc tgtctaacag
    64801 attcatgatt tcttctgttg acaagttcat taattcccaa tcgctttttc cagcaactag
    64861 ttcaggtgtc acttctttca ttgcaagcgg caatgatttg atgttgcggg cataccaaag
    64921 tccttctaag tttgctaaag aaccatcagc aacgatgtgg ccccagccat ctttatagct
    64981 cattaatttt gcaaattcca ttcctacttc ttcttccatt tggcttgttg caggagaaga
    65041 ttcataggca acgttattac cattccatag catagcaaaa ttgtacgcta gtaaagatgg
    65101 catcaatgtt tcagagttca tatgacccca gtaacgtccg gcattatgcc aaggaacgga
    65161 atgggtacgc atccgcgctg aaatttctga tagaacatcc tttgttttat tcacagtgtg
    65221 ttcaaaacta gcactgcttt tttcttctgg cgtaataata ggcatgtctt gaggcatgta
    65281 gttttgacgc cagcctaaat gttcatccac caattcattc aacaaagctt tatagatttg
    65341 gccgttctca gctttgtccc caatgaatag ggcatttagg tttagatctt tcgacaatga
    65401 ttcactcata gtgaaaccct cctaaaaaaa tatttaaatt ttgtcgagcc ctacattaca
    65461 cctgttcttc tattctgtaa actaagtttt ggcctgattt ttaatcatat tttaatatgt
    65521 atcaattatc cgaattgttc acacttttga aataattaaa aaaaacgagg gaatttacaa
    65581 atgtaaactc cctcgcaaag attgtattaa cgacaaccag cgttctattt taggatgggt
    65641 ttcaaaattg gaatgttgta atagtatttc cctttttcaa ggtaaaaact tactaaattc
    65701 taatcaatta ctttggccaa aaagtaattt ttcttacctt ttcgaataac cacaaactta
    65761 ccatcgaatt catcggatgg atttatgaca aaatttaaat cggtaacgcg gtcaccgtta
    65821 atacttatag ctccgtttga aacatcttca cgtgcttgac gtttagatgg ttcaattttt
    65881 gtggaaacaa gcagttccac gatattttca ggtgtgcttg aaatttcaac gttcggcatt
    65941 ttaccgaatc cttgcgcgat ttcttccgca tttagttctt taatatttcc agagaacaat
    66001 gcttgcgtta ttttttgcgc ttctttcaag tcttcttcac tatgcacaaa tcttgtaact
    66061 tcttctgcca aacggcgttg tgcttcacgt ttctcaggtt ctgtctctac ttttttcgct
    66121 aagtcttcga tttcttcttg agacaagaaa gtaaagaatt tcaagtattt cactacatcg
    66181 cgatcatctt gattcaacca gaattggtag aactcaaatg gtgaagtttt cttcggatca
    66241 agccaaacgg caccgcctgc agtttttccg aatttcgttc cgtcagcctt cagcatcaat
    66301 gggatcgtta aaccgaacgc ttctgcttct ggtccttcta attttcggat taggtctaat
    66361 ccagctgtga tatttcccca ttggtctgct ccaccgattt gcaattgcac atcgtatgtt
    66421 ttatgcagat gcaaaaagtc gatagattgc aggatttggt aagtaaactc tgtaaatgag
    66481 attcctactt ccaaacggct ggcaacgata tcttttgcta acatcgtgtt gatgttgaag
    66541 tttttaccgt agtctctcaa aaattcaagt aaagagattt ttgacaacca gtcgtagttg
    66601 ttcacaaatg tgatatttgc atctttacca aacaattttc tcatttgatt ggaaagtgcg
    66661 tccacgttat gttgcacttg ttccatcgtt tgcaacacac gttctgtttt gcggccgctt
    66721 gggtcgccga tcgttcctgt tccgccgcca atcagaatat atggatgatg gcctgccaat
    66781 tggaatcttt tcatcatcat aaatggaatc aaatgtccga tatgcatcga atcaccagtt
    66841 ggatcaacgc cgcaataaag tgaaatgctc ttttcttcaa cgagctcgcg aagtctttct
    66901 tcgtttgttt gctgattaat ggcgtcgcgc caagttaatt catcaataat attcatttta
    66961 ctgacctccg tgtagttttt ttatgaaagc aggaacctgc tagcatgtgt aattaattat
    67021 ttcttcagga taggatagga aataaaaaaa gtcccttggc atataagcca agggacgaaa
    67081 tctcgcggta ccacccaatt tgtatgtcta gatatccaac atacctctcg aacatttaac
    67141 gctgtcgcgt ggcaatcact gccaaagact cctgaaatgt aattcgcttt aaagcatgta
    67201 ctggtttgca gcgaccacca gctttctgaa acattggctt taacaactac ttcgtttcga
    67261 tcttcgtctt gttattatta atatgtgtct atcatagagc gcaacttttt ctttgtcaac
    67321 agaaaaagga tatttttttt aaattcctca ttttttcttt tttgaatccg ctctaattcc
    67381 gaacatttta ttttcaatta ttgttaaaag aaataacttt ttctgtattt tttctgcaat
    67441 ttatcgagaa gtcactttac tttgcctctt attctttctt cgttacaata aggaatggga
    67501 caagtaaaaa aatgaaaaag caagggacga ggtatatgga aaaaaagcaa aaaattattt
    67561 taggagccac ttctgcttta actatcgtta ttttaattct aagtttgttt tttgtcatcc
    67621 gatcatctac aaaaaaagaa ccaatcattc ctgaaaaaaa aataacagaa aaagcaaaag
    67681 aaccgacaga tgataagcaa caactagtta ctcaagcaaa aaatctggct caaagctacg
    67741 attatgacca agcgattgcg ttattggaaa aagatagtaa gaacggatct actgaaacac
    67801 aacagcttct tcaaacacta aaaaaggaga aagaacaact gatcgactgg tctgatccta
    67861 ctcagatctc tcatgtcttt ttccacagct tgatcgtgga cccgcaaaaa gccttccact
    67921 ctcaacaggc acaaggatac aaagattata tggtgactgt tgaagaattc aaccgttcga
    67981 tcgaacaact ttatcaaaat gattttgttt tagttaattt gaatgatttg atccaaaaag
    68041 acgaacaagg aaatctttcc tttaacgggc ttgcgctacc tgaaggaaaa aaaccattgg
    68101 ttctttcaca agatgatgtc agctattatg aatatatgga taattccggt tttcctagcc
    68161 gattggtcgt atcaaataac aatgaaatca aaaatgtcta taaagagaaa gataaagaag
    68221 aaatcggtga ctttgacatg gttcctctga tcgatgcctt tgtcaagaaa catcctgatt
    68281 tttcttatcg aggcgcaaaa ggtaccttgg ccctgacagg ttacaacgga gttttaggtt
    68341 atcgaacatc aaaatctcaa tatggagaca atgaaaaaac aaaaaaagag attaaagaag
    68401 ctaaaaaagt ggcggaacag ttgaagaaag acggctggag ctttgcttcc catacatggg
    68461 gtcacttgaa tatgactcaa gcttcactag aagatgtaaa aaaagacaat gaattgtggc
    68521 agcaagaagt tgcacctatc ctaggaaaaa caaatattct gatttatcca tttggtgcag
    68581 acatcagcga ctggcagcct tattcagcag aaaatcaaaa atttgcttac ttgaagagtc
    68641 aaggtttcga tattttctgt aatgtcgatg cttctacacc agcttgggga caattaggta
    68701 cggatttcta tcgaaatgct cggatcaata tcgatggcat tcgttttcaa tctgatttag
    68761 acggaaaaaa tcctattctt gatccattca tcaatgtcca tgatgtctat gacaaagaag
    68821 cacgcgaatc tgcttgattt tacgtcattt atgaatcgag tagaggggaa agccaactga
    68881 ccttccgccc ctctcacacc accgtacgta cggacccgta tacggcggtt caataagttg
    68941 agtgtacgaa ctggtacttc aaggagatgt cctcatatcc ttggcgggcc agttctttgt
    69001 ctgttaatga tcgatgaaga atatgacttt tggagactga ccaaatacct tttcttgtat
    69061 ttgaccattc atacgctttc gctttatcga tccctaaacg aatcagattt tttcttttgg
    69121 ttcttggatt cttccattgt ttccaaatat attgtctgat tcttcgcttc aaccaaccgt
    69181 ttaactccct catgaattct ttcatctcac caattccata gtagttgatc caaccagtca
    69241 ttagttgctt gatttctttt aaaataagtt ggatacttct tccacggttt cttttagtga
    69301 gttgtttgag cttgcttttc acacgctgtt ttgcttttgc gtgagggcga attttaatac
    69361 ctttctttgt tggcagaatg cagaatccaa gaaactttcg tttcaatgga cttcctaccg
    69421 cactcttttc tcgatttacc gtcaacttta gttgttcttc gagaaattga atggatttct
    69481 tcatcacacg ctgtcctgct cgtttgcttc taacatagat attgcagtca tcggcgtatc
    69541 gaacaaattt atgtcctaga ttttctagca cttgatcaaa ttcgttcagg tagatgttac
    69601 ttaacagggg agagagattg cctccttggg gcgttccttc ttcagttgtt gtgatgagtc
    69661 cattttccat tacaccactc attaagtatt gacggatcag ttttaggact tgtttgtcct
    69721 ttactttccg ctcaatgaaa tacatcagct tatcgtgatt tacggtgtca aagtaggctt
    69781 tcatatcaat gtccaccaca tatcggtaac cttgttcata ataagatttt gctttcttca
    69841 ctgcgtcatg tgcgctgcga tttgggcgaa acccataact attatctgag aattctggtt
    69901 caaatagcgg ttgtaacact tgattgattg cctgttgaag catacgatca ataaccgtgg
    69961 gaattcctag ttttcttaca ccgccgtttg gtttcggtat ttctactcgc aaaacaggct
    70021 gaggtttata gttcccttgg cgtaaattgg atagtaattc ctcgcggtgc tctttcaagt
    70081 aaggaagcag ttgatcgacg gtcatcccat ctgcaccagc cgccccttta tttctgacta
    70141 cttgaagata cgcttgattg agattattcc gatctagaat tctcccaaac agattttcta
    70201 caccatcttg tttcctctgt tcaccgcaag aagtgctgcg cacttcatca tacccttcgt
    70261 gttccacact agctctttga tgatagccaa gttttcttgt tttctgcggt cgtcgcatct
    70321 tctcacacct cctaaattat tcaagattct tattgttcag tccttcgtaa cggtcagtgt
    70381 cgttactact atgacctcgg ctgacttctc atgattcacc atcatgtcgc catgatcgtt
    70441 gtgctggttt gttttcattc aacgcccaca acaccctcat gagatctccc ccggtaagag
    70501 tgaaaacttt cctcccatgt aactgctaga tttactgtat aggtttcggg cagtattgga
    70561 ctttgtcttg tgatgaagac ttatccgacc taattcagcc ttgtatctag tttctgttcg
    70621 tcagttcagg agtttgcgtc cgacttcctt cagccacaac ctcacgattg ccgccttgtc
    70681 tttcgctaac agttcctact gccaagcctg tagtggactt tcaccaccaa gttttcaccc
    70741 atgcagggcg cacaccaaaa aagccgaact gacattttat cgtcagtttc ggctttttta
    70801 tgctttattt cttcttcggt gcgattccct tgatcaggac ttctgtccat tctgctgtat
    70861 atggaagcag ttttgcgtat atctccggct tagaaattcc ttctaccatt gcttgaaaat
    70921 agaggatcaa gaactcatcc gaatatttca aatccacttt tccttctttc cgccctcgat
    70981 ggaaaagatc aagcatgata ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa
    71041 attctttttt ttcttttttt gtatagtatg tcatcaaatc gatataaaat tgtttgttca
    71101 ctcgattgag ataacggatt ttgttttcac tcatggcaat cagtgtttct tcaaatggaa
    71161 gattttccgc aatcaacgct ttcgcttctt gccctaaatc attcataaat gcaaaaaata
    71221 cttcataaat cagcccttcc ttgctgccaa aatatttaaa aatcgtcgct ttacttacct
    71281 gagcgttctc agctacctta tccattgtca ctccagcggt tcctatgtct gtgttcatca
    71341 agtcaaaagc tgcttgtaaa atctctttct ttttctcttg tgctctcttt tcaaaaccgt
    71401 tcatttgtag agcctcccgt aattttaaac ttttataata tatatagttc aaatttattc
    71461 tgaggtttat tataactatc tattgagaaa gtatcaagct agcaattcct tgtttatgaa
    71521 ttcattgaca gaaatttata aaaagaatat tatgtactaa aataataaaa aaagttttat
    71581 aaaaaggtat aaaacctttt tattaaacta tttaatcttg tttagtatat atttttagaa
    71641 aaaggagtgg ggtttttatg gcagaaatcg tcaaagtcac acatttgaaa aagagctttg
    71701 gtcgtttcga agctttaaaa gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat
    71761 ttatcggacc aaatggttct ggaaaatcca cgacgattcg gacactttta gggattataa
    71821 aaaaagacgg cggaacggca acgatttttg ggcaagatgt atgggaagat agtttatcta
    71881 tccatgaacg tctttcttat gttccaggcg atatctcttt gtggggaaat ttgactggag
    71941 gagcgatcat cgatctcttc atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg
    72001 aactgattca acgtttctct cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa
    72061 atcgccaaaa agtgggcttg attgctgctt tggctgttga ttcggatctc tatttatttg
    72121 atgagcctac ttctggttta gatcctttga tggaaacgat tttccaagaa gaagttgaaa
    72181 aattgaaaga acagggcaaa tccatcctac tttcttctca tattctcagt gaagtcgagc
    72241 gactagcgga taaagtcgtg atcatacgcg aaggaacact cgatgaagaa ggaacactcg
    72301 atgaattgcg tcatttgact cgttcgacca tcactataga aacagaagca gagaatagtc
    72361 acttagcaca attgaacggt gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg
    72421 ctgctgataa cgaagctttc gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa
    72481 aatttgaatc tattccaccg actcttgagg atttattcat gagacattat gaaggatgag
    72541 gaggaagcga aatgactgat aaattttcac gctggtccac ccttttttct caaaacctta
    72601 gccgtgactg gaaaaagatc ctgatatggc tagtcggtgt cactgctttt tctggtggtt
    72661 ttgtccctgc gtttgaagaa ttgacttccg gacaaggatt agctggatta tacgaaacct
    72721 tgcaaaaccc ggcaatgatt gcaatggtcg gaccaactcc agcttcgaat gctgctgagt
    72781 atactttagg agcgatgtat gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg
    72841 tgatctctgt tttacacgtg gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg
    72901 aacttattcg ttcctttcga gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa
    72961 gtattctgat caatttgctt ttagcaatat cgatcagttg tacgatgatt tttttccaag
    73021 cggattcgat cactgcagaa ggtgcttggc tttttgggct atctgtaggt agtatgggga
    73081 ttttggggag tgccgttgca ctgtttgctg cacaactgat gccaagttca gccagttcga
    73141 atggtctgtc tttagcgttt ttaggtggac tttaccttct acgagcaata acagatgtag
    73201 ctgattcctc cctttcgatg tggaaacctt taggctggtc ttatttgacc tatccattta
    73261 caactaacaa ctgaattcct ttgctttatt tgtttttgtt cagtctaatc ttgataatca
    73321 gttcatttgt tttagaggaa catcgagatt tgaacaccgg ctatatcccc gaacgtgaag
    73381 gaaaaccgta tgctaaacgt tctcttttat ccttgcgtgg tttattattc tatctaaata
    73441 aagggatgac aatcagttgg cttatcactt ttggtttgat gggggcggca tacggctcta
    73501 tctacgggaa tatgcaacag tttttagaaa gtaacgactt aatgaaacag atgtttatcc
    73561 aatctggcgt ctcaattgaa aaaaacttta caagcacgat catggtcgtc ttgatttgtc
    73621 tagctgctat tttaccaatt gctagcatca atcgtttatt tacagaagaa acccacggac
    73681 gtatgaatca gctgttcgca acaaaaattt ctaggaacca actttattgg accaatatgt
    73741 tactagctat tctatttgct atattggcaa tcctgattag ttctggtgca ctagggcttt
    73801 ctgctttggc ttcgatgaag gatgcgaaag acatggatct attgatgttt cttaaagccg
    73861 ggttcaattt cctgcctgcc gttttagtgt ttatcggaat tagtggcttt ctattcggat
    73921 ggcttcctcg aatcggtaaa cttgcttata tctacttagc ttactccttt gcactcaatt
    73981 actttggcgg aattttggat ttaccagaat ggttttccaa aacagctgtc cctagctggg
    74041 ttccacggat gccaaacgat tcttttgatc tttccgtgtt ccttacgtta tcatgtatca
    74101 gccttgcact gttcgtcctt ggttctattg gttataggcg aagagactta ctagaaggat
    74161 aataaaaaag catgtacaat ttcatgctag cctccaaatc tgggtcaaaa atccaacatt
    74221 tggaggtcat ttttttattt acaagtcttc taactgctct ttatcaatga tccagacttc
    74281 tcgattcttt ctttttacat aaccattctc ctctaataat ttaaattttc tagataaagt
    74341 ttcgggggtc gtaccgataa atgcagcaag ttctttcatt ttcattggca ggaaaaagtg
    74401 atccttccct gtcgctttag ctaagtcaag cagatagaca gcgactcgtt cttctacttt
    74461 ttccatcatc aaaaaagttg tctgctgctg tacttttgtt aatttctcaa cgttgattgc
    74521 taaaagtttc agactaaggg cgggataatg cagtaagagt tccgagaaat ctttttgtct
    74581 caatatgcaa atttccgttt tttctagtgc ttcgccaaat aaagactcat ttggaacgcc
    74641 tagcaactgg ctctctccct catatccgcc cggctctatt actcgtaaaa gctgttcctt
    74701 tccgctagca gataattgat acactttcat actgccggag gccacgatca ctaattgagg
    74761 ttctccattc ggcgcaagaa tctgttctcc tttttccaca tagtgatgtt tgacaagtgc
    74821 gtgtactttt tcttgatctt gctgtactaa gtgattgaac aaaggaacaa atgaaacaca
    74881 taaatgatca tgactcataa tctctcctcc ttctaaacag ctatttctaa taaagaaaca
    74941 taacttccct tattccaact gataattttc ttttatcata gcataaacgt caagcgaacc
    75001 agataccagc aaatagagtg gatttctttt tgtcccttgc tttattttcc ttcttttgag
    75061 accttaagta attgattcct atcaattttt taaatattta ctttttgaat aatcaaaaac
    75121 aaaccttcac tcgcaagccg agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt
    75181 ctattgaaat ttttccgcac ctagcttttg atatagtggt gctagttctt gtaaggtttc
    75241 agcggttatt tgctcaacgt gttcgatttc ttgcggatag aagaatagtt tacccatttg
    75301 aaaggttcgg tctctcatcg cttttttcaa ctcttcttgt ttctttgtat cggacaaata
    75361 gtcagacaaa gctaagtggt cgtcaagttc atgttctggt tgcggccata tgtaataatc
    75421 cttgatatcg actgttttgc tccactcttt cagtatcgca agcaactggt tatacgctgc
    75481 aatgctacca tatcgttctt cgcttttata atgttggtac atcaaataga tttggtattg
    75541 tttttgattc aacaaaactg ctagacaggc attctcgtct tgacgagatt cgcttcggta
    75601 cgcgctccaa aaatgactgc gtaaattcca gccattcgtc cagctttcga ttttaggttt
    75661 gctcatgccg tacatatcag gcaaaagtgc agctgtctgg agctgaatct ctttccattt
    75721 ttgccagtgt tctttatatt ctttcttgat ttgttcaatc ttatctgttg gatggctctc
    75781 tttgagctgt ttgaatgcgt agattggtcg atcaaacaac tgataatctg attttccaag
    75841 ttccattgtt tcgcctcttt ccgttatctt ttgtattcta cctgaatttt gcgatatgga
    75901 caaatagagg ttgtgaaaaa gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa
    75961 ctgcgatgag catcaccgca gttttattga acttaaggtt gattgatcac atctaaaagc
    76021 gttgaaaata aaatggcact agaagcagct cctgggtcga tatgtccgat tgcacgctct
    76081 cctagatagg aagctcgtcc ttttttagct accaagactt tggtgtgttc ttttgcctgg
    76141 tcaatgattt cttttgttaa attccctgcc ttcaatgcct ctgtgactgg cagccaaaca
    76201 tctaacatcg ttttatcgtc agcttctgcc tttcctcggc tttgaatacc cgctgttccc
    76261 tgctcgatca cttcgcctaa tttttcttga gaatcaatag aatcgacctc ttttgtcgct
    76321 ttcatcatgt tcatgaatgc agatccgtat aacggaccag aagccccacc gactttcgat
    76381 atcatcgcca tagagaatac tttgaatgta tcgctgattg tttcaggatg atcggcttga
    76441 aaagcttctt cataagctga aacccctctt cccatgttat ttccatggtc tccatcgcca
    76501 atcggggtat ctagatcact gagatattgt ttgttctgat tgattgcctc tgcaaattgc
    76561 gtgagccatt cttggatttc ttgaacggtt agtttcatat tatcaccctt ttatcttgag
    76621 attttttacc atgaaatcgt ctgtacattg ctttctaaag aatctttcca ttgtgtctcc
    76681 gtcaagtcga tcagtgttag agaaagacct tgcatatcta gtgaggtcat aaaattaccg
    76741 acttttcgga aaactacatt gacgcctctt tcttccaaca gagctaaaac atcattcatg
    76801 aacacgaact gttccatcaa aggcgtcccg cccattccgt tgactagtac acctgcttct
    76861 tttggtaaat tcgagtaatc atctaaaatc ttagtaacaa gttcctttgc cagtacttta
    76921 gaaggctgca tcttttctcg acgatagcct ggctcaccgt ggataccaac accatactcg
    76981 atctcatctt ctcctagttc gaatcctggt ttccctactt ctggtacagt tgctgctcga
    77041 agcgctagac cgatcgtctt agttgcctgt acgacctttt ctcccagttc tttcaactca
    77101 gctagggaag caccgtttct tgctgcatcg cccacgattt tgtgtacaag gacagtacct
    77161 gcaactccgc gttttccagc agtatacgta ctattttcaa cggcgatatc atcatctacg
    77221 atcacacttt ctacttcaat atcgtccata gcagctaatt cctttgccat atcgaagttc
    77281 aatgcatccc ctgtataatt cttaacgatc agcaaaactc cttttccact atcggcttcc
    77341 ttaatagccg tctgaatctg atctggagtt ggagaagtaa acacatcacc aagcaccgct
    77401 gcacttaaca tcccttcccc tacaaacccg gcatgggatg gttcatgtcc gcttcctcca
    77461 ccagaaacca aaccaacttg ttccgctttc tgattcttgg ctacaacacg ggaagattct
    77521 acacgatgaa caagttctgg atagcttttt actaatcctt ccagcatttc ttcgacaata
    77581 ttgcttggat cattcatgat ttttttcaat ttattcccat cctttctttc cggcattttg
    77641 tgcttctgtt aagatttgct gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa
    77701 agctccttcc actaaaggcg catccaccag atgataacgt tgctgctgct cttcttccaa
    77761 catgtctttt gccagttccg agttcaatac cgcgctaccg atatccgcaa aaacaaaata
    77821 actagctgca tcgcttgatt cattgactgc ttctacgatt ttcatcgggt ctgatccgat
    77881 ctcgccattt attccgccga gagaaaaaat ttgcacctct tcggattttt gcatctgctc
    77941 aatcatttct ttaataccat ctgttacttt ttgactatga gaaataagca aaatactttt
    78001 tttcacaaga atcttccttt cttttacata gttgctaacg attggtttcg ctttcattaa
    78061 tgaacaattc aagtatataa taaatttcta attttgggaa aatatgtgta gtgcttcttg
    78121 aagtttgtta tactttaatt aaaagagagg agtgatcgaa tgattacaac aacaacgaac
    78181 agtgtagagg gaaaagccgt agtagcttat aaagggatcg tttttggtga ggtcattact
    78241 gggatccatg catttaaaga cttggaggcg gggttgcgaa atatctttgg cggccgctca
    78301 aaaagctatg aaaacgaact aatgggtgca agagaagaag cacttgccga aatggccgaa
    78361 cgtgcaaaag cattaggagc tgatgcgatc attggcgtaa aaatggacta tgaagtatta
    78421 ggcagcgaca atggcatgct gatggtcaca tgtagtggaa cagctgtctc cacgagataa
    78481 gtaggggaca tctgttttaa taactaa
//
LOCUS       DACCDF010000003        71669 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCDF010000003 DACCDF010000000
VERSION     DACCDF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.003"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..552)
                     /locus_tag="IVK51_000140"
     CDS             complement(<1..552)
                     /locus_tag="IVK51_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP6429766.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(678..2606)
                     /locus_tag="IVK51_000141"
     CDS             complement(678..2606)
                     /locus_tag="IVK51_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6429767.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(2912..3628)
                     /locus_tag="IVK51_000142"
     CDS             complement(2912..3628)
                     /locus_tag="IVK51_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6429768.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IVK51_000143"
     CDS             complement(3629..5452)
                     /gene="glpO"
                     /locus_tag="IVK51_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP6429769.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IVK51_000144"
     CDS             complement(5466..6962)
                     /gene="glpK"
                     /locus_tag="IVK51_000144"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP6429770.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7098..8558)
                     /locus_tag="IVK51_000145"
     CDS             complement(7098..8558)
                     /locus_tag="IVK51_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6429771.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSSFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8548..9843)
                     /locus_tag="IVK51_000146"
     CDS             complement(8548..9843)
                     /locus_tag="IVK51_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6429772.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10175..10852)
                     /locus_tag="IVK51_000147"
     CDS             complement(10175..10852)
                     /locus_tag="IVK51_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP6429773.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(10882..11973)
                     /locus_tag="IVK51_000148"
     CDS             complement(10882..11973)
                     /locus_tag="IVK51_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP6429774.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12074..12508)
                     /locus_tag="IVK51_000149"
     CDS             complement(12074..12508)
                     /locus_tag="IVK51_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6429775.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IVK51_000150"
     CDS             complement(13092..14549)
                     /gene="ascB"
                     /locus_tag="IVK51_000150"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6429776.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14546..16441)
                     /locus_tag="IVK51_000151"
     CDS             complement(14546..16441)
                     /locus_tag="IVK51_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429777.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16499..18274)
                     /locus_tag="IVK51_000152"
     CDS             complement(16499..18274)
                     /locus_tag="IVK51_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6429778.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18324..19820)
                     /locus_tag="IVK51_000153"
     CDS             complement(18324..19820)
                     /locus_tag="IVK51_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6429779.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20014..21636)
                     /locus_tag="IVK51_000154"
     CDS             complement(20014..21636)
                     /locus_tag="IVK51_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6429780.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(21964..23277)
                     /locus_tag="IVK51_000155"
     CDS             complement(21964..23277)
                     /locus_tag="IVK51_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6429781.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23522..23746)
                     /locus_tag="IVK51_000156"
     CDS             complement(23522..23746)
                     /locus_tag="IVK51_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429782.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(23766..24134)
                     /locus_tag="IVK51_000157"
     CDS             complement(23766..24134)
                     /locus_tag="IVK51_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6429783.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24330..25040)
                     /locus_tag="IVK51_000158"
     CDS             complement(24330..25040)
                     /locus_tag="IVK51_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429784.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25058..25555)
                     /locus_tag="IVK51_000159"
     CDS             complement(25058..25555)
                     /locus_tag="IVK51_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429785.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25592..26044)
                     /locus_tag="IVK51_000160"
     CDS             complement(25592..26044)
                     /locus_tag="IVK51_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6429786.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26179..26784)
                     /locus_tag="IVK51_000161"
     CDS             complement(26179..26784)
                     /locus_tag="IVK51_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6429787.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27234..27854)
                     /locus_tag="IVK51_000162"
     CDS             complement(27234..27854)
                     /locus_tag="IVK51_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6429788.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(27856..28725)
                     /locus_tag="IVK51_000163"
     CDS             complement(27856..28725)
                     /locus_tag="IVK51_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP6429789.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(28748..29155)
                     /locus_tag="IVK51_000164"
     CDS             complement(28748..29155)
                     /locus_tag="IVK51_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6429790.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29174..30463)
                     /locus_tag="IVK51_000165"
     CDS             complement(29174..30463)
                     /locus_tag="IVK51_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6429791.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30490..30792)
                     /locus_tag="IVK51_000166"
     CDS             complement(30490..30792)
                     /locus_tag="IVK51_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6429792.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(30817..32910)
                     /locus_tag="IVK51_000167"
     CDS             complement(30817..32910)
                     /locus_tag="IVK51_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6429793.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33399..34163)
                     /locus_tag="IVK51_000168"
     CDS             complement(33399..34163)
                     /locus_tag="IVK51_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP6429794.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34274..35143)
                     /gene="mdcB"
                     /locus_tag="IVK51_000169"
     CDS             complement(34274..35143)
                     /gene="mdcB"
                     /locus_tag="IVK51_000169"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP6429795.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35168..36244)
                     /locus_tag="IVK51_000170"
     CDS             complement(35168..36244)
                     /locus_tag="IVK51_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP6429796.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36268..36861)
                     /locus_tag="IVK51_000171"
     CDS             complement(36268..36861)
                     /locus_tag="IVK51_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP6429797.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(36865..38541)
                     /gene="mdcD"
                     /locus_tag="IVK51_000172"
     CDS             complement(36865..38541)
                     /gene="mdcD"
                     /locus_tag="IVK51_000172"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP6429798.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(38531..38830)
                     /locus_tag="IVK51_000173"
     CDS             complement(38531..38830)
                     /locus_tag="IVK51_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP6429799.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(38932..40578)
                     /gene="mdcA"
                     /locus_tag="IVK51_000174"
     CDS             complement(38932..40578)
                     /gene="mdcA"
                     /locus_tag="IVK51_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP6429800.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(40585..41496)
                     /locus_tag="IVK51_000175"
     CDS             complement(40585..41496)
                     /locus_tag="IVK51_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6429801.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            41616..42473
                     /locus_tag="IVK51_000176"
     CDS             41616..42473
                     /locus_tag="IVK51_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6429802.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(42660..43022)
                     /locus_tag="IVK51_000177"
     CDS             complement(42660..43022)
                     /locus_tag="IVK51_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429803.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43368..44807
                     /locus_tag="IVK51_000178"
     CDS             43368..44807
                     /locus_tag="IVK51_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6429804.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(44814..47366)
                     /locus_tag="IVK51_000179"
     CDS             complement(44814..47366)
                     /locus_tag="IVK51_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP6429805.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47480..48811)
                     /locus_tag="IVK51_000180"
     CDS             complement(47480..48811)
                     /locus_tag="IVK51_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6429806.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(48777..50255)
                     /locus_tag="IVK51_000181"
     CDS             complement(48777..50255)
                     /locus_tag="IVK51_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6429807.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(50560..52395)
                     /gene="tdc"
                     /locus_tag="IVK51_000182"
     CDS             complement(50560..52395)
                     /gene="tdc"
                     /locus_tag="IVK51_000182"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP6429808.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            52865..54292
                     /locus_tag="IVK51_000183"
     CDS             52865..54292
                     /locus_tag="IVK51_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6429809.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54384..54749)
                     /locus_tag="IVK51_000184"
     CDS             complement(54384..54749)
                     /locus_tag="IVK51_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6429810.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(54746..55111)
                     /locus_tag="IVK51_000185"
     CDS             complement(54746..55111)
                     /locus_tag="IVK51_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP6429811.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55303..55551)
                     /locus_tag="IVK51_000186"
     CDS             complement(55303..55551)
                     /locus_tag="IVK51_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429812.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            55550..55678
                     /locus_tag="IVK51_000187"
     CDS             55550..55678
                     /locus_tag="IVK51_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP6429813.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            55735..57429
                     /locus_tag="IVK51_000188"
     CDS             55735..57429
                     /locus_tag="IVK51_000188"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP6429814.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            57433..57552
                     /locus_tag="IVK51_000189"
                     /pseudo
     CDS             57433..57552
                     /locus_tag="IVK51_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            57634..57825
                     /locus_tag="IVK51_000190"
     CDS             57634..57825
                     /locus_tag="IVK51_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429815.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            58203..59336
                     /locus_tag="IVK51_000191"
     CDS             58203..59336
                     /locus_tag="IVK51_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP6429816.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59367..59687
                     /locus_tag="IVK51_000192"
     CDS             59367..59687
                     /locus_tag="IVK51_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429817.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(59716..60180)
                     /locus_tag="IVK51_000193"
     CDS             complement(59716..60180)
                     /locus_tag="IVK51_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429818.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60311..61564)
                     /locus_tag="IVK51_000194"
     CDS             complement(60311..61564)
                     /locus_tag="IVK51_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6429819.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(61593..61910)
                     /locus_tag="IVK51_000195"
     CDS             complement(61593..61910)
                     /locus_tag="IVK51_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6429820.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(61936..62256)
                     /locus_tag="IVK51_000196"
     CDS             complement(61936..62256)
                     /locus_tag="IVK51_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6429821.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(62720..64135)
                     /locus_tag="IVK51_000197"
     CDS             complement(62720..64135)
                     /locus_tag="IVK51_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6429822.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64152..65468)
                     /locus_tag="IVK51_000198"
     CDS             complement(64152..65468)
                     /locus_tag="IVK51_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6429823.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(65618..66316)
                     /locus_tag="IVK51_000199"
     CDS             complement(65618..66316)
                     /locus_tag="IVK51_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP6429824.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(66605..67594)
                     /locus_tag="IVK51_000200"
     CDS             complement(66605..67594)
                     /locus_tag="IVK51_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP6429825.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(67674..68507)
                     /gene="msrA"
                     /locus_tag="IVK51_000201"
     CDS             complement(67674..68507)
                     /gene="msrA"
                     /locus_tag="IVK51_000201"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6429826.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(68682..70316)
                     /locus_tag="IVK51_000202"
     CDS             complement(68682..70316)
                     /locus_tag="IVK51_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429827.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(70303..71637)
                     /locus_tag="IVK51_000203"
     CDS             complement(70303..71637)
                     /locus_tag="IVK51_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429828.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        21132 a        15102 c        11980 g        23455 t
ORIGIN      
        1 ttttctttga aacatctttc ggtactcgac agcatacaag acaaaagatt gtaaaagacc
       61 gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt ggtaattctg ggaaaagcag
      121 caaaaggatc atgccaacat agatgacagc agtcgctact ttgccatacc acttcgctcc
      181 acttaatttg atatttttag atagcccgat taatccagct acaaacataa agccttcttt
      241 tatcaagaat agaccaaaca acagccaaag ccacggtcga taccatgcca tagacaaaat
      301 cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc accttcccta agtctgttac
      361 ctgattatat ctccgagcaa tgaccccgtc tagataatca gacaaacctg agacaacaat
      421 gaccgctcct gctatataaa atgactgtct cagcacaaaa taaagataaa tcggcagcaa
      481 aaaaatacgg aatatcgata aaagattcgg aatcgtttgt atttctttac gccagtctgt
      541 gcttaatttc attttatcgc cctacctcct cagcactctt ttctttttat tttagcttac
      601 acggggaata aaaccaatga tttcactgtt tttatttttc tttacagcca ctgatattat
      661 gagctaattt gagataatca cttgctgttt tcatcgttta ctcttctttc ttcctgtcgt
      721 ttgcgcattt cttttactag tactttatga gctttttttg atcctcgctc taggtcacgc
      781 tgccgtttac gtgttttata atcgataaac agactgtcta aatcgtaacc tgtcgggtac
      841 agatcttctg ctgaacgcac cagacgaaca cgctgacgag gcacgtggat tatttttttg
      901 tctaaatata catcaatcgt atcttcacct atatcttcat aaactaacgc ttctttttgg
      961 tattcattga caaatatccg atcacctttt gcaaagagct ggcgttcttt tttattctcc
     1021 ttcctatttg taactgctgt cttaggaaga aattcttttt tctgtgtccg ataggctttt
     1081 tccgccaagt aacgctctgc ttgatggatc aatttcatcg acatatccat tttatgagca
     1141 atccacaaag cttggctttc tcctgtttct ccaacccgca actgatattt aggacgaagt
     1201 gcttcccgat cgaaagccat agcagccggc acaaaatctt catgatctcc ggcgaatttc
     1261 ttgatttctc cataatgggt cgtggcaatg atcaatgccc cttgctcata catagattcc
     1321 atgattgcga tcgctagtgc agccccttca tttggctctg ttccactgcc aatctcatct
     1381 aaaagtacca acgtattcct ttttatcttt ttcaaaatag cagcgatgtt ttgcatatga
     1441 ccagaaaatg tactcaacgc attttctaaa ttttggtggt caccgacatc gacgaaaatc
     1501 tcatcaaaga cagcaagctc tgtcccttct tttgccggaa cttgcatacc gaacatcgcc
     1561 atcaaagtca ataaaccgac tgtcttcaac acgactgttt tgcctccggc atttgctccg
     1621 gtgataatca agcctcgata gtctttaccg atttcaacat ccaatggtac cgcatgctca
     1681 ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt ccgatttatt gattctaggt
     1741 gtgcttccat tgatttctcg gctaaatttt gcccgagcaa agatgatatc taacaccgtg
     1801 atcgtttcga tcaataaatc gatcacttct tcgttttctg cgatagcacc agtcaatgcc
     1861 gccaggactt gatattcttc actgatttct tcagctttta acaactgata gtgttcattc
     1921 aatttagaaa tcacagttgg ttcaataaaa accgtcgttc ttttatttga ttcatcgata
     1981 atcgttccat ctattttatt tttatagctg gctttgatag gaatcgtata gtattcgcct
     2041 ttttgaacaa tcattgcttc ttggatcatt tctttgtttt tcggatgacg caagaatttc
     2101 agtaattttg actggatttc tttttcgatt agttgtaatt gtttgcgtac tttacgcaaa
     2161 ttacgtgacg cgtcgtcact gactttatta ttttttattt tctggtcaat caactcttct
     2221 acatttatca aatcaggaag atgtttgcta taagcaaaca acaagggtgt ctggtattga
     2281 ttcttatcaa aaaactttgt gatcatccga ctgcttctaa gaaaatccgc atattcgatc
     2341 aaatcggttg gctgcagaat caaccctttt ttgacttgtt ctgtcagtgc atcgatcctc
     2401 ggcaacccca taaatggaac atgctgactg ctttctaaga tcaagcgtgc ttctcttgtt
     2461 tcttcttgac gatccaagac agtttgtagg tttgtcgaga ccgtcatatc gctgattcgt
     2521 tttttgctat agtttccaat tgctcttgct tggatttcct ttttgatccg atcaaattgt
     2581 aattgactaa tcatttcgtt gtccatattc tctccttttc ttgaagagat acctgtccca
     2641 aaataaataa gggcctagag ataaataaaa ggatagaaca cagcttaaaa aagtcatgtc
     2701 ctatccttat tagaatacaa agatattgtt ttcctgacag gtaccgtttc ataagcacac
     2761 caaataaact ttgtttattg gctattcgac ttactgatta gataccagct acactcacaa
     2821 aacatacccc attcttctag gtcttattta ttttccactt aactatagca agacacattt
     2881 tggttgtcaa actttagtat gttttataga tttacaccat taatgaatag acacctaccg
     2941 cgataattgc tccgatcatt ggacctgcga ttggtaccca tgaatatgcc caatctgaag
     3001 ttcctttatt agctattggc aataccgcat gtgctaatcg aggaccaaag tcccgagcag
     3061 ggttgattgc atatcctgtt ggccctccta atgaaagacc gatggctaag atcaatccac
     3121 ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg tccaaaagct agcaacccaa
     3181 gaactaatac aaatgtacca attaattctg tgatcacatt tgctggataa ttccgtatag
     3241 caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc tttcgttgca ttccaatgtg
     3301 gcaaataaga aagccaaaca agaatggccc ctgcaaaagc tcctaaaact tgcgcaataa
     3361 tgaaaggaac gacaagattc caactaattg cccctgtcat cgccatagct agagagactg
     3421 caggattcaa atgagctggg cccatgaatc cagaaatata gacagccatt gttacagcga
     3481 gtccccaacc aaatgcgatg acaatccagc cagaagcctg tgctttactt ttgtttaaat
     3541 tcacagccgc acacacacca tctccaagca aaactaggat cagtgtccca ataaactccc
     3601 ccatgatttg agtcatatca ctgttcattt atttctcccc ctttagttcc cgcagatctg
     3661 attctgcaat caatgcttct aaacgtttag tctgttgcgc cttctcctct tctgtccatt
     3721 caaaatatcc agcaattgca tcgatcacag gttgcttgat ctcatcaagc tgatctcgtt
     3781 caaacaacag atgatttgta cgacgaatga gataatcgcc tggggccaaa accatttctt
     3841 cttctaaacc ataacgtaac cgagctgact cagcgagact caaaccagga taaggtgcca
     3901 tttcttttgc taattcaaag atacgtcggg cattagtccc gtaaaaatca gcgatataag
     3961 tagcatcttc ttcctctaaa ccggctgcta ctccttcttt tgctaattct gtcactgttt
     4021 cttctaattt agttggatcg aagtttccac ctgaaatctg atatttttta gagtcgattt
     4081 ctttcgtctc tattccgtat tcttctttta ataattggcg tatcaatcgc aaagctcctt
     4141 ctgccatctt acggtaatcg gtgattttcc caccagataa agtcagcaaa ccatctggtt
     4201 ctctttctaa agaactacct ctggaaatcg tagaaggtgc tttttcatca cggctgtttt
     4261 ccaaatggtt caacacgtct tctacttcag cacgagaaac tttgttttcc ttatattcac
     4321 ttactgtatc gacaacttta ttgaagctct tgtctgaaat cgatccatta tctccaccat
     4381 tgtaatcaga accagaatta ccaatcaaca gtggacgaag tcctgcccag cttgcttcga
     4441 tatcattcaa tgtgatattt gcttctggat agcgatgatt gatcacatcc aatagataat
     4501 ctacatcttc ttgtgtgact ttcggatccg taaaatctcc ttggtaatcc gtatctgtcg
     4561 taccaaagta agtcttgttt tctcttggaa tagcaaaaac catccgccca tcttgctttc
     4621 ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt cgcatctaca actaaatgga
     4681 tccctttagt tggacgcatt ttaggagaga ctggacgcgt aaaattcaag tttcgtactt
     4741 tatctaccca aggaccactc gtattgatca ctaattttgc tttgatttcg atcacttcat
     4801 ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc gccatcatat aaaaagccaa
     4861 ccgctttcat tttacttact agataagccc catcttcggc agcctttttg atattatcaa
     4921 tcactaaacg ggcatcattg ttgcggaaat ctagatagac acccgcgcct tttagccctt
     4981 cttttttcaa aaacggttct ctttccaata cttcttctgg tgtcagcgta taattctcat
     5041 atttggttcc tgtcacattt gccagtttgt cataaaggtc cattgctact ttgaccgaga
     5101 acatattgaa ggttgttgct ccttcgccct catagattgg caaaagcatt ggatctggtt
     5161 ttgggatgtg tggggcaata ctttgtacga ctgcacgttc accaactgtg tcagctacta
     5221 cttccacatc aaatgtcttc agataacgaa taccgccatg aacaagtttg gtcgagcgag
     5281 aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc tgttttgatt cctgatgctg
     5341 ctgactggac tgccacaccg gcaccagtga ttccgccacc gatgatcaac agatccaatt
     5401 cttcttgctg cattttttga atgctatctt gtcttgtctt gtttgaaaac atatggattt
     5461 cctccttatt caaatgcttg ggtagctttt actgcttttt tccaaccgtt gtaaagtttt
     5521 tctcggcgct cttcctccat ttgaacttca aatagcttgc cagcttcata aaattcgcgg
     5581 atctcgtctg tatctttcca ataaccgaca gctaatcctg ctaaaaatgc tgcccctagc
     5641 gccgttgttt ccaaattttc tgctcgttgg atcggtacat tcaagatatc tgtttggaat
     5701 tgcatcagat attcattgtt tgctgcaccg ccatctactt tcaatacagg aatcttgatt
     5761 ccagtgtcat cttgcatcgt atcgatgata tctctcactt gataagcaat cgactgtaaa
     5821 gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca agccaaacat agcccctcgt
     5881 gctgcctgat cccaataagg agcacctagc ccaacgaatg caggaacaac ataaacctca
     5941 tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc tagctttttg caacatttga
     6001 agtccatctc tcagccattg aatcgaagaa ccagccacaa aaatacttcc ttctaaagca
     6061 tagtaaacct ttccatttat gccgtaacca attgtcgtca ataaattatt tttggataat
     6121 tgcggttttt caccggtatt catgacaata aatgaacctg taccataagt attcttgacc
     6181 atgcctggtt cgaatgccat ttgtccgaat aatgcggctt gctggtctcc tgccatccct
     6241 gaaattggtg cttcgcttcc ataaaaatga taaccttgcg tataaccata aacttctgag
     6301 ttagaagtga cttttggtaa catcactcga ggaatattta gtaaatctaa aatctcctga
     6361 tcccaatcta gctgatggat attgaacaac atcgtccgac tggcattcga gtaatcagta
     6421 acatgtgctt gtccatcggt cagtttccat actaaccaag aatcgatcgt tccaaacatc
     6481 aattcgcctt tttcagctcg ttcttgcgcg ccttctacat gatcaagaat ccagcgtatc
     6541 ttcgttgctg agaaataggc atcgatcact aaccctgttt tttcgtggaa aaattcttga
     6601 tagccttcat tttttaagtt atctgcaatt ccagctgact gtctggattg ccaaacaact
     6661 gcatgataga ttggacgtcc tgtatctttt tcccatatta ctgttgtttc acgctggttc
     6721 gttatgccaa tcccagcaat ctgatttggt ttgatccctg attcgatgaa cgctccggca
     6781 ataactgact gtacagaatt ccagatttca tttgcatcat gttccaccca actttcttgc
     6841 gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt gtcttccttt cttgtcaaag
     6901 ataattgctc gtgagctagt tgtaccttgg tcaatcgcca tgatatacgt tgattctgtc
     6961 attgtgattc cctccattat atgcaaacgc tttctttaac tactcttatc atactactgt
     7021 tttaaaaaaa gaaactgtca aaattttatt ctttttttct ttttttgaca gttttttatt
     7081 atgaagctat tttttaatca attcttttct tttttcagcg tctctattct tcttttcaat
     7141 cgaataatgt cgtaaggtga cgcaaattct gacagataat attcacactg ttttttgttt
     7201 ctctggcaaa atgtgatcac caaatcatat tctttacctt cttcataact tccaatctca
     7261 ataccgccga ttcccctcag ttctctgatc agatattgtt gaaaagcaat acgataaatc
     7321 ggcagacttt ctaaatcgat tccaatcact actggttttg caatataaaa atcgatcatc
     7381 aaaagaacat tggcatactg gatcatcagg taactaagct cactatcttc agctcttttc
     7441 ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca gtaactgtgt taaggaatgc
     7501 ccgagcattt tctgctgctg ttccaaaagc cgatcacgct catagacttc gatctttcca
     7561 acaaagaaaa accattcatt gttgatctgg gaaatttggt agcctactgc cttttcttct
     7621 ttgattttca aacgacgagg acgataatgc cataacatcg tttcccgaat gtaagtatct
     7681 aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat actgataaaa tatttcttcg
     7741 tctactatcg aaaaagaaac aaaagagctg taaaacatca ttgtttcgta tacattcagt
     7801 tctgctgctg ttcgactaag atgcatcacg atgattggat cgattgcttg ataaagccgg
     7861 tccgactgat agagtaattt cttttctttc aaagttgcgt acgtactttt ttcattcaat
     7921 agtctttttt tcgtaatccc gagccagcaa gcaatctgtg cttctgctga tgccgtaaac
     7981 gttgtttcta atacacggtc caatcctaga atgaacggtc ttttttctgg tgtattttgc
     8041 aaaaaaagcg gtcgctgatc ttccgggata tattggaata gttcataata aaaatatcgt
     8101 atctgtagct cttctccgta taattgaccg tttttgattt gtaatccaaa ttcctctaag
     8161 agctggttca attctttgat ctttcgaaaa acagaagact cactgatatt aaacgctgtc
     8221 gctaatcgaa cgatggatac ttgctgatgt tctaataaat agacgagcat cttaaacttc
     8281 aagctttcct gcaataaaaa caatagaatt ttttccaaac ttacatcttc gtccaacaca
     8341 agcagcgcct gatactcatt tcgctgtact tctacttttt tttccatcat tcggccaatc
     8401 aattcaatat cttccaaata ttgatcaaac gaggcttttg acagttggac atgctctctc
     8461 aaaccattga tagaggctgt tcccccttgc agaacaaggt ttcgcaaaat caggatctgt
     8521 aactgctctt ttctttctaa taatgcttca atcttcatag gcagtattcg ccacctttga
     8581 ccacaccata tctggcacac ggtattctgg ctggaaaaat agctcatgtt cttctaactg
     8641 ttcaaacgtc catccttctt ttactgcaat tgtcagttgg tcaagaaggt ggaacatctc
     8701 ttttttgctt tggagctgtg ctccaagaat ctttttttca gaatcaaata gaaatttgag
     8761 tttaactttg ctttgattta ttgctgtaag cttgtagtct acgtatcctg tttcaatttt
     8821 ttggggataa aaaatgctct cttcttctgt caaacctacg cttcctaaga aatagccaaa
     8881 ccactgattg ccgactggac gataggttcc ctgatccttt gtctgtgagc cactaattgt
     8941 cttgctgaca tttcgtgctg tacggacagc gttatttact agagaaacat acattttttc
     9001 atttgttgga cgaaaagtta cttggatcgc atcgccaatc gcataaacgt ctttttgaga
     9061 cgtttgcaag tagtcatcta cccagatcgt tccatcatca ttcaatgtta attgttcttc
     9121 ccacatctga ttgtcggggc gagaatgatt agccagcaaa acaagatcat agttctcctt
     9181 tcttttttcc gtctctacaa tagcggtttc attttcggtc aaagacacaa cttgttcttc
     9241 taaaaaaagt gaaagccctt gatttttcat ttcctttact aacggttctg tcatttcagg
     9301 atctagataa cgaaaaagaa ttgactttcg gctttcgtaa agatggattt gtttttttct
     9361 tgatgccaac ccttcagcta tttcgattcc tgcttgtcct gcaccaataa ttgcaatttt
     9421 ctttgcattc atgatctttt gctgaagagc cgttacgttt tgtatctttt taactgggtg
     9481 aataagcgtt gattctgcag aaatatttcg aaacttttga tttgaaccag tcgctagaat
     9541 caaacgatca aaatccaatt tttttcctcc tgctaaaacg actttttttt ctttcaatcc
     9601 tattactgtc ttttctgtgt aaacatggat tcgatagcgg tcagtcagtt cttctttcgt
     9661 gatccaagaa agttgttttt cactggtaac ctttcctttt aatatcaaat tgaacccgct
     9721 gggaatccat cctaacttcg attgcttttc aatcaaatat atttcacttg ttggcatcaa
     9781 ttggcgaaga taaatagccg catgaatccc tgcaaacgac ccgccgacaa ttacaatgtt
     9841 catctgcatt cctctttctt ataaggtgtt ctactgctaa cttctcaatc agaaaacaca
     9901 gtattcataa ctcgctttca accttatgat acatagcgat gcagcagaat caaccctctc
     9961 catcataccg tgttctaggt ctgcttctca ataatgtact agtggaccta attgaagagt
    10021 atcatttctg aagagatgtt tcaaaaaatg tactcttata aaagaaaaaa actcgccttt
    10081 tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa agacgagttt tagcgtgtgt
    10141 aatgtaatag ttagaataga cacatcattt atttttaatt ctttaatgct tcaacaactg
    10201 cttcgcctaa ttgatgagca gattgaggat tttgccctgt aagaagccgc ccatcaattt
    10261 gaaccacttc tgaaaaagct gctccagact gatagtttgc tcctttttct ttcaaagcat
    10321 cttctgctaa gaatggtact tcattcgttg taccatttgc ttcctcttct tcattactga
    10381 acccagtcac tgtacgtcca gctaaaatag attttccaga ctcatctgta actggcaaca
    10441 atcctacaac accgtgacaa acagctgaga ccagcccttc atttcgatag atttgtgaag
    10501 cgactttccc aagatctatt gcttctggaa aatcccacat tgttccgtgg ccaccagcat
    10561 aataaaccgc tgcataatca tttgcatcca catcatcagg acgtaaggta tttcccaagg
    10621 cccgatttct gaaatcttca tcttcataat aggcccaatc gatttcatcc atattcaaaa
    10681 cactttgcgg ttctaaaggt acatatccgc ctttaggact gacaaaatct gcatcgatcc
    10741 cattttttac taatacatca taaaaatgag tcagttcact taaccacaaa cctgtaggac
    10801 gctcgatctt ttcgtacttt tctacatttg ttaatacgat caatactttc attttcccat
    10861 cctctttcta taaaaattgc gttattgata acgtgctttc aagaaattat ataatgggct
    10921 tggcaaataa agctctttat atgcttcttc aaaaggtttc gttacaaatt catcctcacg
    10981 gttttccaat aatttctctg cccagtcagg atcgacgatc atttgctgtc caattgctgc
    11041 aagttctgca tatttcaata cctgctctac atcttcgcga ttgcggatcc caccgacacc
    11101 aactaaaggg atctttcctt gaatcgtgtt atggaccact tcaagaatcg atttatcgtt
    11161 gtatttatca gaacgagcta ttcgatcata catatttaat gaaacatgca gataatctag
    11221 ttttgactca cgtaatcgtt caagaagcca gatcgtgtct tcaaaacgga tacctggtgt
    11281 ttcatattct tctggtgaga aacgataccc tacgatgaat ggttttttag catattgttc
    11341 aaccgctgag aaaaccgttg ttaataattc ttcaataaaa cgataacgat tttccaagct
    11401 accgccccat tgatcctctc gtctattcga atgcggagag aaaaattgtt ggatcaaata
    11461 cgtatttgct ccatgcaact cgattccatc aaatcctgat tcaatcgctc tacgtgtggc
    11521 tttgccaaat gcttggattg tttcaacgat ttcttcttca ctcattgctc gtggtgtttc
    11581 cgcatcagga cgttttgctg caactgcact tgcagataca ggctgttctc cagataaagt
    11641 tgctcgactt gtcattcgtc caccgtggaa aatctgaata atcgcttttg cgccttctcc
    11701 ttggattgct ttcgctagct cacttaatcg aggaatcatt tcatcatgag cgatgctgag
    11761 ttcacctggc cagccttttc cgatatcttg gacatttgca gcaccagtaa taacagcacc
    11821 aagaccttta gaacgttgtt tataataagt gatctcatct tcggtgatca ctccattgaa
    11881 aaagctgagt tgtgtcgtca taggcgacag catcaatcgg ttcttcaatt gaactcctga
    11941 aggtaaagta attgattgat caaattgtga cattgtcatt cctcccataa ttcgctctga
    12001 ttaacctatc gagaatcatc tatcattaaa ccactcgatt agttgtacat gcaactaatt
    12061 tgcttaaaaa attttatttt ttttgtaact tatctgttgc atcattcatt gtctgggcta
    12121 attctttact tgcttcattt cccaaaactg ctttgtatga ctgatgaagt tcttcccagc
    12181 taccatcgac ttccttagca aattggcgtc cttgttctgt aagataaact aatgacaagc
    12241 gaccttcgct aactgttttg acgtatcctt tatattctaa tttttcaaca aaacgagcaa
    12301 tcgttgatgg ggcggaatag agcatttgtg caatatcttt ttgtggtatt ccatcttttt
    12361 cttttaatat caataaaata aaaccataag ttggtgccag acctgtcctt ctaaacgctt
    12421 catcagctaa tttattcaac atgcgatcta gctttttcac gctaaagaaa agacattcat
    12481 ctaacgcttt ttcaattggc ttttccattt catcccctcc attggcgtac atatcatagt
    12541 atgattttta ttttttgtca aaaaacacgg aataatgtcc gcctcctctg tctagatttg
    12601 aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt ttcgacaaaa ctccttctgg
    12661 aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt tgagtatgaa gaaaaatttt
    12721 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgaaaa actggctttt
    12781 gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg catcaagtaa taagaacagc
    12841 caaacaaaaa tagttcctca tattttgtcg ttttttgagc taatgccgat ggtcagctga
    12901 ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga actccgagtc acaaggagca
    12961 atttcaaaaa acagcttctc atgttttatg actatggtga cttgtgatcg aggagttggc
    13021 aggagaatac tgtttattcg taaagaaggg gctgtgacaa gacttttgtc acagtccctt
    13081 atttcctttt attataggat ttcgccattt gattggatga cttgtctgta ccaatcaaat
    13141 gattttttct ttaaccgttt ctttgtgcca ttcccttcgt catcttgatc aacatagata
    13201 tatccataac gttttgacat ttgtgaagta gaagcgctga cgatatcaat gcagccccac
    13261 gacgtataac caaataaatc tactccatct ttgatagctt ccttcatctg ctcaatatgc
    13321 tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc catcttcttc tactttgtct
    13381 atagctccga gtccgttttc agctatgaac agtggttttt gatacctatc atataattga
    13441 ttcaaagcaa ttctcaaacc cttaggatca atctgccagc cccattcact tgatggaaga
    13501 tacggatttt taattcctcc agttattaag tttccttgga ccccttccca attttctgga
    13561 tgagcactga ctgcactaga catgtagtag ctaaaagaaa caaaatcaac agggtaggct
    13621 gctaagattt cttcatcacc tacttctatt tttaagttga cacctttttc ttcaaacatt
    13681 cgttgcgtat acgctggata atatcctctt gcttgaacat cactaaagaa caaagaagtt
    13741 ctttgctgct caattgtttt ttgaatatca tccggatcac aagaatacgg aacaagaagc
    13801 tgatagctga tcatacaacc gacttgtgaa cctttgataa tttcatgaca agcttttgta
    13861 gctaaagcac ttgctataaa ctgatggtgt gcaccttggt atttcgcttg ttccaaatgg
    13921 ggatggtttt cttcaattac tcctacgcta acaaaagggt gatgttttac acagttgatc
    13981 tcattgaaag tcatccaata ttttacttta tttttatagc gtttgaatac tgtttcagcg
    14041 aaacgaacgt aaaactgaat aagctttcga ttaggccatc caccatattc tgtaaccaaa
    14101 tataacggca tttcataatg ggaaagagaa acgactggct caatcccata ttttagacat
    14161 tcgtcaaaca cttgatcata aaaaaataat cccgcttcat tgggtgtttc ttcatcacca
    14221 tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc atttaaaacc catttcagca
    14281 aataaacgga tgtcttcttt aaagtgatga tagaaatcat ttccgtgtct tttaggataa
    14341 taatacttgc tgttgggatc ctgcgccgct tttacttgct gatgcgtcat catatgccat
    14401 tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg tatacacatc agacacagat
    14461 aatccttttt catctacatc ccatgcacct tcacattgat tggccgctgt cgctccgccc
    14521 cataaaaatc cttctggaaa ttttttcata tcgatccccc taaacttgct agttttcttg
    14581 tttgattatt tttcttatga ctagtaatga tatttttgaa tctactacta tttgtaacaa
    14641 taaccattgt ggtcggatca tatccctctt tgattaattc ttgcaaatct acttccatca
    14701 ataaatcgcc tttacgtact ttttttccat cagatactgc actttgaaag tatttgccat
    14761 ttaaattcac tgtatcaata ccgatatgga tcagtatctc tgctccttct tcactgacta
    14821 ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc cccgtttaca gaactgtaga
    14881 tttttccgtc cgttggaatg atcgcaaacc cttctcctaa agatccacta gcaaatgttg
    14941 gatcgtttac ttcagataaa tcaattattt cacctgctgc aggagtactt aacactatat
    15001 cctttgagga ttcttcttcg atttcatcct tgtagccgaa aagaaaagtt agcatacagg
    15061 aactgatcat cgctaagaaa taactagcaa ctagccaaac aaatccttca ccaatataaa
    15121 tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc accggctcct gcggcaccag
    15181 ctactccacc aaatatagct ccacatatac aagcaatgta aaatggtcgt ttatatttaa
    15241 gagtcacacc ataaacagca ggttcagtga ttccaaataa tgctgtgata aatgcagata
    15301 aagataaact ttttactttc ttatttttcg ttttcaaaaa taccccaagt actgctcctg
    15361 cttgcgcagc tacggcagtc atacatgctg gacctagcgt atcacgaccg tagaaagaaa
    15421 gattgttggt cattactggt acaagtcccc aatgtaaccc aaaaatcact aacacttgcc
    15481 aaaatccgcc gataacagca ccagataaaa cagggctaaa gttataaaca gctaaatatc
    15541 cagctgcaat cagctgactt gcataatctg caattggacc tacaactaat aaaataattg
    15601 ggaccatcac gatgatacaa atgaatggtg tcaataaatt tcgaatactt tcgtgcaagc
    15661 ttttgcgaat cttaggctca atataagaaa gaacccatac agctaaaatt gctggaataa
    15721 ccgtacttgt ataccttgct agaatcacgg gcatccctag aaatcgcaag gttatgctat
    15781 cactatatgc actgacaatc gtaggataaa cgagagctgc tgcaataacc atagcgatga
    15841 aacgatctgt attgaacttt tttgcagcag tatatgccaa aaatataggc aagaaataga
    15901 aaaatgcatc agctgctgca ttcaaaatga tataagttcc tgattcagta gttaataaat
    15961 tcaaactcgt acataaggcg agtactcctt tgatcaaacc acttcctgcc aagacgccta
    16021 aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga gtttaaaatg ccttttttct
    16081 cagttcctct ctttccacta gatgcatcat cttctagttt catttgtttg gataatacgt
    16141 catacacttc atttactgca tttcctacta cgacttgaaa cattcctcca gcttcgacta
    16201 cagtgatcac accttctagt tgctctacct tctccttatc tgccttctta ttatctttta
    16261 gggtaaagcg taaccttgtt gcacaatggt aaacattgct gacattttct tcgcctccga
    16321 caaatcggac aatatctttc gctaattgtg aatagtccat ttttacttcc cccttgttaa
    16381 ttaatgccgt agcaatcctt cttacaagat aagtatagtt cttagaaaac ggtttctcca
    16441 ttcaattagt accccttcta atagcgaacg aaactgtaca atagtgtgta tggtttactt
    16501 agccgagcaa atcactcact gcttgaaaga aaacaagtat atcgtttctc ttatagtcaa
    16561 aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg tataagtggt atctcaagct
    16621 gatactctgt gatttttgtt tctacttggc ttttgataag acagcacaac aaagtctcgg
    16681 catttaacgg aagcataact aggtcagaac taggagagat tagttgctga atggaatcta
    16741 tctttttatg gatttttatt tgattttctt ctttgtagta atgatcaagc agttttgttt
    16801 tctttttttc ttcaactatg gtcagctttt cacgcaaaat ctgataaatc tcccaagatt
    16861 caaactcatc tagaacacta ggtagataaa tcaaatcatc ccactcaaaa ataagctctt
    16921 gctcagtagt caaaattgca tcatattccg ttattttgtt tgattgattt ttgaaaagat
    16981 acactggttc tatccggcat tctttgatcc acatgttcat actttgttga atctgttttt
    17041 ttagtgtatt caaaatacta attggctggt tactaacaat cagtatctcc ccttgttttc
    17101 gaaaaggatt caacgcttcg gcaagataca aaacaaaata agcgctttca gctaaattgg
    17161 gacgtaattg aaaatgtatt gggaagaaac gccgaattaa ataaatttct aaaggataac
    17221 gggagatgat cttttgtaca aaatgattca tggtattatg tccggcatcc atcctcgttt
    17281 tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg gaacaataac gaaacaggaa
    17341 gatctaaaaa acgaataatc gtttgctcaa atgctcttag attcttctct atttctttgt
    17401 ttttttcatc attaatcatc aaataattta actctaatag tcgattttca attgcttgtt
    17461 tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa cttttttgtg agggtctcta
    17521 ccatcggaat aaataatgta tacctgtttg ttttttctaa aagttcttct cttcgaagaa
    17581 tggtcaaaac gagataccga gaaaatcgta tgaagtcctc tcctgaaaca atataattat
    17641 atgatataag aacctctttt aatgtctttt ggacagtcgg catgatattg gaaaaaattg
    17701 tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt ttctgttcca acaagagata
    17761 agaaaattct ttttgaattt tcttcagcaa tgatttttag tccttttgtc gacgaaactt
    17821 ccagttctaa tttaggattg cgctcgatcc aacgcaaaat cgtttttatt tgtaatgata
    17881 cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg catactgaca tacccttttt
    17941 gaaacaataa gtacgtcaca atagttgaag ctcggaaatc catgctggaa tagattttat
    18001 gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga ttcaattgat tctaaaaaat
    18061 aacctgaacg tttatttgac tggataatga ttttcttctc gtagatagca agcatatttt
    18121 tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc ttcagctaat tcttcgcttg
    18181 ttatccaatg attcttattt cttaatacta caagaatttg gagttgtcgt ttatttaagt
    18241 ccatttgctt acacctcctc tttgttataa acatacagga aaaacctctc aaatgacaat
    18301 agtcacttgc agaggtttaa aaatcatttg aagaaagatc tatattcaag gtttttacca
    18361 ttagactgga taatttgttt ataatattca aaactatctt ttttcaatct cctaaagctt
    18421 ccgctcccat cattttgtct atccacataa ataaagccat atcgcttcct catttcccct
    18481 gttcctgccg atacgatatc gattggtccc caccataagt aaccaatgat ttggcaacca
    18541 tcttttattg cctcagcaat ttcctgaaga tgtattttta gatactcaca acgttttgga
    18601 tcgtggattc gattcaattc atctggtgct tcatctagtc caatgccatt ttcaactatg
    18661 aataatggta attcataacg gtctgtcaat tcattcatga cataacgaaa ccctagagga
    18721 tcaattggcc aactccaagg ttcgggagat ttttccttca aaaagggatt atcgaaacca
    18781 tttgctccgc cagtatctac gcgcatttct gcttcttttt tatagacagc gctacgataa
    18841 taactaaatg caatataatc tgccgtgtta gcttgcaagc actgttcgtc tccatctaac
    18901 attactggtg cacagtcttc ttccttccat gtcctgtaaa tataagctgg ataatgccct
    18961 cggcacatga catcggagaa aaaccatgaa ttgcgtttaa attgcagtga accaaataca
    19021 ttctctggat cacagtcata tgcataagtc gctaatgcag aaaagctaca cataataccc
    19081 atcttagcat ttggatcgat tttctttaat aattgagtag cacgggcgtt cgctagaaac
    19141 atatgatgac aggcttgata aatcactttt tttgttagtg tagcaatggg ttcaagttta
    19201 tctctagcag ggaatgtaac caatccagca gctgcataag gcatacgaaa agcattattg
    19261 atctcattaa aggtcatcca atatttcact ttcccttggt agcgttcaaa gacggtctgc
    19321 gcataaaatt caaaaaagtc gattaatttt cgattttgcc accccccata ctctcctacc
    19381 aatgctaagg gggtttcata atgactaaga gtaattactg gctcgatatc atatcgattt
    19441 aatgtattga ttagtctgtc ataaaatacg agaccagcct catttggctt ctgttcgtct
    19501 cctcttggaa atattcgtgc ccatgagatg cttgttcgaa aagcttttaa acctaactcc
    19561 ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat caatcgcttc atgagaaaga
    19621 taatggagtg attcgtctaa agcaatctcc caaatctttt tttcttcatt ccactgaata
    19681 ctaggatgct ggttcatgat cccgataagc gtatctgtca cattaggtaa tttgccgtct
    19741 tccaaccatg caccttcaca ttgattagca gctactgctc cgccccataa aaaattttct
    19801 ggaaatattt cctgatccat aactatctct cctcgctatc aacaatttct tacttgaagt
    19861 atagctcgat tcatcaaaac aagatattct aactatttcg gcagaagaga agaaaaaaat
    19921 tgaatgctac tcttcatttt taatatttat atataacaaa tacataagac gatccccgaa
    19981 agaaaacact cgttaaccat ctaagcaaac gatttatttt acttccaaca caaatgcttc
    20041 ataaggtcgt aaatcaactt tgccgtctgg taactgtttc agaggataat ttgacacaat
    20101 aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt tcactggaca tattacaaat
    20161 aacgagccat tgctggtctt catatatgcg agaataggca aagacatgct caggcgtctt
    20221 ttccactaat tcatagtctc cccagacgat gatgtcatgt tttctacgca aatcgatcaa
    20281 tttcttgtac atataaaaaa cagaatcttg atctgctaac gcttcttctg cattgatttc
    20341 tttataattt ggattgactc gaagccatgg aattccagta gtaaacccag catgttcacg
    20401 ggcgttccat tgcataggcg tcctagcatt gtctcttcct ttcgccttga ttgattccaa
    20461 gatttcctct tttgtaaatc cagaagaaat cctttcgtta tacatattga ttgtctcaat
    20521 atcttttgct tccgcgatat catcgattgg gcaattcgtc atgcctaact cttctccttg
    20581 atagatataa ggggtgcctt tcatcatatg gagcaagata gcaaacatct tggcactttc
    20641 ttttcgatac acatcgtcat ttccccaacg agaaacaatt cgcggaagat cgtgattatt
    20701 ccaaaataaa ctattccatc cttcatcacc taaagatgtt tgccatttgg ataatacttt
    20761 tttcagttca gagatactca agggtttcaa atcccatttg tctttgcctt cttgctgatc
    20821 taatcctatg tgttcgaatt ggaagaccat ggataattca tttcttttag gattggaata
    20881 aagtttagcg atttcggtag ttgctcccca tgtttcgcca acagtcatca gatttttgtc
    20941 accaaaagtt gcttgattca tttcctgaag atactcatgc agtttaggtc cattacttgt
    21001 gatttcttta tccggttctt tgccaatcat atcgatcacg tccatacgaa agcccccaat
    21061 acctttatcg atccagaaat tcatcatttg ataaatctct tggcgaacct gtttattttt
    21121 ccaattcaaa tcaggttgac gcttgctata caagtgcaga aaatattgcc ccgttttttc
    21181 atccaactgc caagcagaac cactgaatat tgagcgcaat ctattaggct cttttccatt
    21241 gaccggatca cgccaaatat aatagtcccg ataaggattt tctttatttt tacgggcctc
    21301 aatgaaccac ggatgttcat cagaagtatg attcaccacc agatccatga tgatacggat
    21361 atttcttttc tttgcttctt caatcagttc ttccatttct tccatcgtac cgaattctgc
    21421 catgattgct tgataattac tgatatcata cccattgtca tcattcggag actcgtatac
    21481 tggagaaagc caaattgcat caatacctaa atacgccaga taatccaacc tggaaataat
    21541 cccccgcaaa tctccaatac catcttgatt tgaatcttga aaacttcttg gatagatttg
    21601 ataaacaact gaatggtgcc accatttttg ttccatgatt ttcctcctat aaatggatgt
    21661 ttctattttt tgcttaatca tttattttag tctctctcac taaaaattgt aacgctttca
    21721 aatagctatt gcaccttgtt atcggtaaca gaataaaaca aaaaaacagc tgaggaaatt
    21781 ccctcaactg ttttttgaaa ggattgattt atgcagggaa gaatgctgat ttcacaactt
    21841 ctttataaac ggtgttcact cagctgtccc ttggtattag ctcaaaaaac ggcaaaatat
    21901 aagaaactat tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc
    21961 tctttatttc acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat
    22021 gattggagat acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa
    22081 acctaatgca atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa
    22141 tgaagggatc gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa
    22201 tactcctacc ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg
    22261 agaaaagaac aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg
    22321 tcctccaacg gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa
    22381 taaggaaccc gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat
    22441 cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc
    22501 aaagaaagtc acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc
    22561 ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat
    22621 ttcagaaggc attggaatca aacctgtgac cagacctaaa atcgttgtag ctgccatccc
    22681 gatgaagatg ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa
    22741 aatcgtcaac caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt
    22801 cgaaacgatg atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc
    22861 agaaatcgca tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa
    22921 gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca
    22981 tggaatcccc attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg
    23041 tgccgttcca attggataac gggctaatac ccccattaaa acacagccga tcgcactggc
    23101 taatgcagtc gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg
    23161 atttacaaat aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg
    23221 ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt
    23281 ccatccccct aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa
    23341 aacaacgtgt tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta
    23401 agcaaaatcc gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca
    23461 ctttggatta caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa
    23521 atcaatagtg tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg
    23581 tatcgacagg attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc
    23641 cttttgtctt cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg
    23701 ctcgccgata cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt
    23761 tttattcaat cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa
    23821 aaattgaaag tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg
    23881 tagatagttt caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct
    23941 ggtgaaaact tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg
    24001 ctgagtacaa tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca
    24061 attttttcta caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt
    24121 gttttttgta tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact
    24181 gattccatta tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa
    24241 agcaatagtt tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaacggcaa
    24301 tgaatgccgc tcttcttatt tcgattcttt tataattctg cttctgattc atctttggtt
    24361 agtgtgtgtc taacactagt tacgccaaat gtttgatcga ttttttcctt caacgcttcg
    24421 attgcctgtg cgccacgtgc cgcttgttca gcattgatgg attcaatgac cagcaccgct
    24481 gcttgtgttt cttccgtcag catcgttctt tgggcttcac gccagttgag acagcgacag
    24541 atcgctcctt gttcatcata gtagatgatt tcttccggaa gtgccggagc atcggaatct
    24601 gcacctaaag gcaggaatga ttcgccacct tgggcttttc ctaaatgcag atttcctgcg
    24661 atcttttcga tatcttctcc gccgcaaggt acgccaaaag acaaggaaat actattatag
    24721 atatctacaa gcggattgat tgggaaaaat tcccgttctt gatttgcacg tttcagtaaa
    24781 gcttcaatag aagaacgtgc acctttttta gtcttgaatg tggtaaatgc ctgacgccac
    24841 tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa ggtaatttct tgcttctttt
    24901 gctgcctcat tcaataattg atgatacgtt tcttcacttt cttttgttac gtggttatcg
    24961 ataccattta cggataagat ttcgatggtt gcttcaggaa atacttccca gaaattggta
    25021 tccactatta cttttttcat ctgttcacac ctcttgatta gaataattgc ttgatgaata
    25081 gatagtctgt ttggggatca tctcccatat aaaaaacatg ttcgctgtat ttgataaatc
    25141 cttttttacg ataaaaagcg agcgcaacat cgttgtgttc ccaaacaccg agccaaatgg
    25201 ctgttttttg cttttctttt gctttttcaa tagctgtatc tagtaaaaat gtaccgatac
    25261 cttgatgttt aaattttttt cgtacataga ttcgctcgat ttctaaaaaa tgatcagcca
    25321 tttcctcaga ttgactcgtt cctgtattta gtttcaggta acccgcaggc tcttgatcga
    25381 tactcactag gaaaaaatcg gtttctttat cttttagctc ttctgtaagt ttttctagtg
    25441 cataagcttg tgtcaagtaa tcagctaaat gctccggtga attatctttt ccaaatgtat
    25501 ctgtaaatgt ttcgatacta atttgttgta gtaagtctac atctgaaggc gtcatttttc
    25561 gaatttcgta tttattcacg gcttcttcct ttcaatagat ccttttattg ccttttttga
    25621 caaaattcca atcttcgcta acattcttgc atactttatt taaaagatat tccaattgtt
    25681 ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt ggagtagttg ttttctcgaa
    25741 tgatgaaagg atagatttct gctccttttt tagtcggata gagtttcttg atttttttat
    25801 tttctttatc ttccaaacgt tcgatcatcc cattggattc taatttcttg attgctcggg
    25861 cagtcgttgt acgatctact ttgatcattt ctgctagttt ttcttggatg attccaggat
    25921 tttcgcaaat acgtactaaa taaagatact gtcctcttgt taaatcgact tctttgaatt
    25981 cgatattgct aatagaatct agcgctcggg caatcattcc gatgtctcga aggatttcag
    26041 ccatttcatc ccacctttat tttgtttgta agactagttc acctcttttt gttgcaaatg
    26101 caactatttt caataaaaaa atcaagttct tttttaataa ctagtaaagt atattctttt
    26161 cttcttttac gaaaaagctc aatcacaata gatctggttt ttgttttcta aaataagtaa
    26221 ctgtttcttc gttgttcgct tttctgatag ctttggcaac tcttctatac taaaaaatcc
    26281 catgttggaa gtttcattat tctcaataaa cttggcctct tcaccgtgga tcgcacaagc
    26341 aaaaacaagt ttataatact gaaaaagctg tggaatatct ttttgtttat tcgtatcaaa
    26401 aactgctcgc aactgagtag tttctactgt taatcctgtt tcttcataga cttctttacg
    26461 cacattttct tcaggagaca atcctatttc agcaaatcca ccaggaagtg accattcttt
    26521 cgtccgttta tcttcgatta gtaggacctt gccatctttt ttgatatagg cacgaacatc
    26581 tacttttggt gttggatgtc cttctccaat cgttgtgagc gcttttattt cttcgaaagt
    26641 ttcttttcct atttcactga tcagttccaa tgaaatcgtc cggatctcct caaaacgctc
    26701 tttatcaaat acatcttttc cataatatag accagcttga gaaagtgcga gtagtctttt
    26761 gtattgagcc aacttcttgt tcattctctt cccttctttt ttagcaaatg tatgactatg
    26821 taatcaagta tagaagactg gtttaattac ggcaaataaa tataatgaaa tctgcaataa
    26881 ataaaagaat gttaaaaaac tcgagaacaa aagacattcg acttctctgg ggctgtgaat
    26941 aaatggcgtt caaaagcttg catctgcggt aataagcccc accaaacgaa aaatatgacg
    27001 agctattctt gtctggctgt tcttattaat tgaggcagga cagctttttc acaacctccc
    27061 cactaaacgg tgttctccta ccaactcctc ggtcacaagt cacaatattc atcaaacatg
    27121 agaagctgtt tttttgaaat tgctccttgt gactcggagc tgaacgacag gctcacgacc
    27181 tctcggtaaa cagcgttcaa aagccaattt ttcgatatta agccatttct ttgttactta
    27241 ttgtcattga tttctttttc taatttatcc aatagtggag tgtaatccac ttgagtatag
    27301 tctgttacat caaattcata tagtgttcct aactggaatt tgtcatattt tcggtcggcg
    27361 atgatctttt tgaatgcttc tttcgtaata tgattggtcc ctttcgttcg atcttccaat
    27421 acgtcaccat agtgataatg atccatgata tagctcaaat gacgatctga ttcacgatca
    27481 cgtttgtacc gacgttgcca taacacttca aagtctgctg ttaggcgaat cgtgatcacc
    27541 tgatatccgt atctatctgc taattcttgg agtcgtgctt tttgcttatc actaaaagga
    27601 tactcggaca caatcacttt ctttcctgct tccatataca aatcaagtgc tttgtaatag
    27661 aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt ccaaattgtt aaacccgatg
    27721 gattctgcat agtattcctt gatttcatcg agcgtgatgg catacatatc tggaaatcgt
    27781 tcccgtagtt tattcataag atatgttttt cctgtaccag gactaccagc cagaagaatg
    27841 aaatactttt tcatctcatt tatttcctaa gttaattgga ctttccaagt tcataaacag
    27901 tccaatctta tgcttgacaa tttcttttac tgcttcattt gctggaatca cattatggcg
    27961 aagagacttg ttgatttctg tttcactaaa ggtttcttta gctactttcg tccagttgac
    28021 taaaagctcc gttccaacat tgaatttgcg gatcccgtta ttgatcaatt caggataatc
    28081 ttcttctttc acacctgttc ctccatggat aactaggggg acatcaacta ctgcatcgat
    28141 ttctttcagc aagggaatgt ttacttctgt ttttgatttg aattgtccat gattcgtacc
    28201 gatagcaata gctaatgcat ccacgcctgt agcttctaca aatttcactg cgtcttccgg
    28261 tcttgtatat accttgtcat tttcagcgac atggattcct tcttctgtac cgccgattgt
    28321 tccaagttct ccttctacgg aaacaccgtg cgcatgggca tattctacta ctgcttttgt
    28381 cttcaggata ttttctttga aaggcagatg ggacccatca tacatgacag aagtatatcc
    28441 gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg tggtctaagt gcaagactgc
    28501 atcaaccatt tcttcttctg caatagctgt cattacattg actaacacat gcatgccgat
    28561 atatttagcg gtatcgacac ttgtttgaat aatgatcggc gaacccattt cttttgctgc
    28621 gcctagcata gcaggaagca tttctaggtt atgcgtatta aaagctccaa ctgtaaaatt
    28681 caactcttct gctgtttttg ttacttcttt caatgttgta tacatctttt attgccacct
    28741 tatcttttta tttgtttcct ttgatcgtta cttgttttgc aatttgtcgc atctcatcca
    28801 ttttgcccat gtagtaatcg atcccttgat catcaccagc acgagcagca tctgcacgct
    28861 tctggttata gaggctcatc agttttttat acccatctcc gatcgtctgt ttattttcca
    28921 aactcttttc taaatattgg atggcttgat cgttcacata caattcagaa aatttaagtc
    28981 caacttgttc ataaagtttc gctaatttat gcttatcttg ggtagtggaa atctcttgtt
    29041 tcaattgtgt aatctctgat tgaatctttt cggtcgcttc tggcgatagg gctttttctt
    29101 cttttgccag aacttttttc ggttcttttt cttttttctt aaaaaatcca aacatcattt
    29161 tccctacttt caattaaaat gctttcaaaa ctgggcttaa gatccaggca tataagagtg
    29221 ctgcggctac tgtatctaca tcggttgctg tagcattgtt gaatcccatc gaattaaaga
    29281 tcgttactag caaagcgggc aataatgtaa taaagaaacc atgagcaata ccgccaatga
    29341 tcgctcctcg gcgtccgcca actgcgttgc caaatatccc tgctgtccct ccggcaaaga
    29401 aattggttag cataccaggt aaaatcatcg ctaagccgaa catgggcaaa gtaaacatgg
    29461 caataatcgt accaatcgtt gtcgtaatga aacctaaaat gacagcattt gggctataag
    29521 gaaagaaaac tggacaatct aaggcaggga tcgcatcagg aactaatttc atagctatcc
    29581 ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac accagctagc aatacataca
    29641 cacccactac gaattggatg gcttgtaaaa aggcaaacat gatatagttt tgatctcctg
    29701 ataattcaga agcgaatggt tcgcctgcaa ataaaacagt gatgatatat agcgggacca
    29761 tcacgaccat gacagacaaa taagtatctt gcagaaattc gaaatgtgtc ggcaacttga
    29821 tatcttcgat cgacttttta ttttcgcctt tctcaccaaa aagcttagca acaccggctt
    29881 caaataagta cccgatcgta caaaaatgac ctaaagcaat atcatttgat ccagtgattt
    29941 tacgaatgat cggttgcgct actgccggca tagcaatggc gaaacaagct ccgataaaac
    30001 caccgaccaa gatcaatacg atccctcgca gaccggcaaa atagccaaag actgtcgtca
    30061 tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat gtacttccat tttgtgaaac
    30121 gagccaaaat aatattgaat acaaaaatgg ctaagaacgt cagtgcaata tctctaccta
    30181 aacccaaatc attcattgct tgaccattga tagattcaat agaagggatg atcccttgca
    30241 tatgaaatcc ttcagtaaag attttcccga aataagttaa acttccaacg atgatgctcg
    30301 aaccggcact caacacttga aatcctagga gtgtctttaa cgtgccagag atgacttgtc
    30361 ctgctgattt cttctgcagt agtagaccca acatagcaat caatgcaatg gtgatcgatg
    30421 cctgagtcaa aatattttcg atgataaaat tgataacgcc catgttttta ccctcttttc
    30481 tttttgtatt tagataactc ccttatcttg cagaactggg gtgatttttt cttcaatttc
    30541 attttttgaa acaatatttt tcaaatagat catcgccgtt ttaggatcaa ctgcgaactt
    30601 ttcgaactgc gaacgaaaat tttctgctgt gatgatcaca tctggtttca tggaaactgc
    30661 ggaagaaata tcacaatggt ccagtttggc ttctactcct aatttgttca aaacatcctc
    30721 agcagacatc tgtgctgcaa aactacttcc taaacctgca ccgcatacga ataaaatgtt
    30781 caatttgttc atctttgttc tcccccgtat catttattat ttctcaacta ctgaaaaaag
    30841 cagttgtttg aaatcttcga tatttgtgca ttcaatcaat cgatccaact tctcttcgtt
    30901 gttgatcaat tcaattagtt ctttcatgat gttcagatgc gagtaggagt ctactgctgc
    30961 caaacaaaaa ataatcttta ctggatccat ttcaggatta ccgaaatcga ttggttgctc
    31021 aatcgtagct acgctgactc ctaatttatt gaccccatct tctggtcggg catgggctag
    31081 tgccatgtgt ttgccaatca cgatatacgg tccgtaatca ttcactgcac gaatcatcgc
    31141 ttcgacatag cgctcttcga caatgttttc tttaaccaat ggtctagcag ctagttcaat
    31201 cgattcctcc caagtagtgg ctttttcttt gatcatgata ttgctgtccg tcaaaatatc
    31261 ttttaacata ggctggatct cccttttgtt gatttctaaa ttgtttgttt cgaataattt
    31321 ttctaacttg ccatagatga ttcgatctac tcggccacca ctttctttca tgacatctat
    31381 tatttgagtg aataactccg tcgattcatg actgttaggc tctaaccgct gtagttcttc
    31441 atttcttttc aaaaagtctt ctataaccaa gatattcgtc tcagttaaaa tcggatcgac
    31501 aactaaaagc ggcttatttc gataattcaa ttgaaaggtg ctgaaaacca gattgacatc
    31561 caatttatct actaattcga tttcttttga acttaaaact gcagtaatct cgatctgagg
    31621 aaacttttct ttcagattct ctgctaacag atttccagta gccatcccat ggttacagac
    31681 aacgactgat ttataaacat aattcaagtc tcgcttgatt acactggcga tcgtcgaaaa
    31741 gtggatgaca agaaaagcta tctcgtcctc cagtatcttg ccaccaatta cttcttcgat
    31801 cgtaggaaca aaatgagtga ctgcgcggta gattgggaaa taatttgttc ggatgttttc
    31861 tttcaatgga ttcatgactt ggatatgatt cttgattctt acaatcagtg cagccatgtg
    31921 cttatagaga ttttcgaata aagcttcttc ctttagatgg aaaggtacgt gtgtctcgtt
    31981 ctctacaaat tggatcaatt ggatcgttag taattgagct tgtacccact cgattgaatt
    32041 ggagatatct ctagcattga acgtctcaat agtgaaacga agataatttc tttctacttg
    32101 cgggacattg tccaattgaa attcctcgat catgtggtca ataaatgcag aaaagctact
    32161 gtcttttatt tctgtatttt tccaattaat ggcagagata aattcatgtt tcctgattcg
    32221 ctggatccag atcaatgtaa aaagcaggat ttgtttttta taaatatcat cttcttgttt
    32281 caacaactta ttggaataga tctcctgtat ttttttgatt tccaccaaag gaaaataatg
    32341 gagaaagatt ttttgagccg gtgttaattg tgcatcttga ttcataaaga agtcggtcat
    32401 tcccagattc tgattgataa aatcatagat catcgtacgg atagatcgtt ctgaaccttt
    32461 ataaaccagt ccttgctttc cataactgac gatctctatc ccataactga ccagtgttgc
    32521 tcgtaaccgt ctcatatctt catctaatgt acttttagag atttgaaatt cttcttcttt
    32581 tttgtttaga tggatcggga ttcggctgta agcaacggct aaaactagat caaaaatacg
    32641 ttcgcgccga ttcaaaaagc cgtctttcac atcttttatt tcagtttcta atcgagcttg
    32701 cttttccttt tccagcttca acaaaaaacc tttgttgcga attgtacgga tctccggaag
    32761 ctttttactg ctcaaccaga tattcgcttc aagaacttcc tgacggatcg tccgctgact
    32821 gacattgaac tcacttgcta gatgttttgc ttttactgga tattgggctg tcatgattct
    32881 gtgaagtatt tcaattgttc ttgttttcat agaaatgatc tccttctgat ttcaagtata
    32941 tggttccgct tacataataa aaagtagata tttctgccac ttgaagtgga aagaaagtaa
    33001 gcaaagcaaa caaaaaataa gccatatgct ttttcatctc ctcaacgaaa aagacatacg
    33061 cctattatta caaaaatttt gcccatctat acaaaaaaga ggttgtgaaa aagctgtcct
    33121 gcatcaagta ataagaacaa ctaaactaaa atagtgcttc ctatttttcg tttggttgtt
    33181 cttattatcg cagatgcaag cttttaaaca ccatttagtg gagaggttgt gacagagatg
    33241 ttcagccttg agcgataaga agcgattttg aaaaatcgct tcccatattt tacaaaatcg
    33301 tgccttattg ccgataggct atctctggca caccgtttat tcagaaaaag gagctgcgac
    33361 aaggcttttg tcacaactcc gttcataaat atttcctgtc aatccagcat aatcggctga
    33421 ataaaatcgg cttgccatct tttttctttc tcagaaaaat caaaatacca tatctgagaa
    33481 aaatactgtc gttgtgtctt acttcctgag atcaatcgcc catcttttcg atcgactgaa
    33541 taatcacagc atctgaaatc aattcggatg gcaaaactat tttcgtggat ctgtcgataa
    33601 tttcctactt cctgaatagt tattttttta gtatgatttc ttacaccgtc tgctttgttt
    33661 ttttgcagaa ctttctgatg ttcggcaaac agcttgtctg tataacagct tctggctttt
    33721 tcaggagttt cttcttccca agcggattgg atcttcatga atatcttttg gatttccaga
    33781 aactgtgctt tcttttcttt agccgttcct gacatgcggg acaatatctc cgctttcagc
    33841 cttttctttt gtcttttatt cttgacgata gaaaaagcag aaacccctcc tactgacaaa
    33901 acagctaatg caagaaatcc cgtttgctca taactagaag gacctgtacc tggaccataa
    33961 tacccataag aatctctgcc gtaagaacga ctgccaaaat aactgccccc atatgaacga
    34021 ccaccaaatg aacctctgcc cgaacttcga ccgctagatg tacctccacc atgaccaccc
    34081 gcgacagcga atccttgggt cggcagagca aacaaacata ggcccactaa caaaatgatt
    34141 gctaaataat tgattttttt cattgtcttc ctcgttatct tttattacaa aaaactattt
    34201 ttatgatagc cttgctaaaa ggaagagtca ataaccagat gcataaactg tttttttagc
    34261 aattttgtct tatttagtca ttgagtaaca tcggacaaat gtatgtgtcg gcagtccata
    34321 attcctctat cttcagcatg aacaagcttg cagacaagag atctgcgctt cctccaggag
    34381 agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa tggacgattt ggtaaagcac
    34441 tttgactagc agcatgagca agtttttgca tttccactaa cgttttctga tcactgcgat
    34501 gcaatacgca tgtgtcatct agcgaactca tcaaaactaa caacatatgc atttgtttct
    34561 gtacatatga atcattatct agctccttag ctgcttgaat tgcaagcctg atatgcggat
    34621 accctaatat cgcttcttca tatgcaccat ttacgccata tttttgcttc accctatgac
    34681 catgagtggt ttttttatga cgatattttg tatcaggaaa agcagcaagc ttgctgacat
    34741 catcaagtat tttcgataaa gaaaaaggct gttccatact gattttttgt gcaatgacac
    34801 tgatcaatag ccccattgcc caaatcgctc ctttatgggt attgatgcca tttgtagcaa
    34861 tcagcattgc cttttccgct tttcgcccaa tctcagcaat tctttctctt aactcctgat
    34921 caatagcatg atgccatgct atttgagcca tttcttcaaa aaaaggagtt agcgtcttag
    34981 cagacttaag aaataaagca tagtccatat cggaatgtgc acccgcatcc agtgcatcca
    35041 ctaatccagg ttttggactt aatgttactt ctgcgatcaa tgcttcctct accaacgcag
    35101 cgagttgctg agtcactgct ttttttcgtt cgtcatttat catgacactc actccttctt
    35161 cctactatta cttgctgaaa ccttttttga tagctaccca aatgatatga agtaaccagc
    35221 ttaaagcaaa tgcacaactt gccgtcatta gataagctac aagaatcatg actcctgaag
    35281 gtaactgcag ccaactatct ttaaacaata ataacagtac ttggaaaaca aatacattgg
    35341 ctaaatacgt acgataagca taaacagaca accatttgaa caatggcaat tttggcgaat
    35401 cgtttacaat cattttggaa gcgcctatga agacagcaaa gatgatgact aaactgtata
    35461 aactttggat cgtattcaag taaggtgcat tcgcaaagct caagtctcct ggatgtgcga
    35521 gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac aggtaagaaa agataacgta
    35581 ctctgtccaa atattgatag actttttcgt gataaacaaa tgcggctacc ccataaatca
    35641 tgaaactagc aacaaaccga tctaataagt accagctgtc atgataaggc ccagtcaata
    35701 cttgtgtttg atagaacaga taccatgcga catataaaat cgttgaacca atcaatactg
    35761 gccatacata ttttttatta gcaaatactt tgtaaccgag ccaaacaaaa aacggcatga
    35821 acaattggat ctgcagcatc ataacggtat accataaatg aggcgctcca tcacctgtga
    35881 catatttcag taaaaagctt gcagtatccg tatacggagt ttgttgttgg actgctggaa
    35941 atagccaaag ataagccgtc gtccatagaa gatacggaac gactagttca aaaaactttt
    36001 ctttcaaaaa ttcccaataa gacaaatgct gacttgtttt taccatatta taaacaattg
    36061 caaaaataaa cattggtgct gtgtatttaa cgatgacgta aaaggtacca agaaaagctg
    36121 tatttgttgc gctgtggctg tttgccaaag caaagctcaa tatcgtttgc atcataacag
    36181 ccgttacagc tgatactttc tggaagtcac ttacgttgat tttgattttt ttgttttcaa
    36241 acatctcttt ctctctcttt ttctttatta ccaaagttct ttggttaatt taggtccttc
    36301 gactgttttc aatagaattt ttttctctgg ctctctcaaa tactctttta gtgaaaaacc
    36361 gccttgccga gtcactactt gggtatcgaa ttgctcaaaa aaagcaggat gcgattgaat
    36421 tacctgaatt ggcaattcaa tcggtgaatt tgcgtacagc agcaaatcaa aatcactggt
    36481 ttcttttact gccggaatcc cagtcgataa ttcaaatcct aagctgccgc cgattcccca
    36541 tttacatcct gataatatgt ttctagcagc atgaaattgc tgataaacag gataatccat
    36601 gattttctct tgtctatacg attcttgttc gatgatgtcc cacggatgaa tgacttgttt
    36661 gacggcatgt tttgagattt ctgttgacca tctctgattg cgggcaggtc cacgtaaacc
    36721 aatcggtagt atttctgcgt gatccgtcga cgcacgacga actgttccga acatcttttc
    36781 agttagaacg acccattcag gcaaggtgaa cccttctatt tcatgtttat caaaaaggac
    36841 aatatcatgt gcttgaatca ttttttatgc atcccattct tcgtccattt tggcacggac
    36901 tttatttgtt tcgacacgtc cgaatgtttt ggctgtttcg tttgtgtatc gatttgtcag
    36961 catcgtgtca tttgtattgc ggacatcttg aatggcttca ttgattgctt cgatgacttt
    37021 gctgacattt tgttctgaag cagcttggtc ggtgatatct tctaggaaat gatagatcgc
    37081 tcctagtttt gtataattat tgatatcgta agcgatagct ggaacttttt cagcagctgc
    37141 ttcgatttca gcaaccgaac gttttgtcac acgtgcagca ctttctttag acatcgcttg
    37201 gacggtaatt tctttattgt ttaaagcaat caagcggctt gattgtaatc catgtgctaa
    37261 aaatgcacct gaaatagcat ttcctgcaat caatccaata actggatgtc cagcttgacg
    37321 gagttttgca taagcagcag cactattagc caaagccaca tggataccga tcaattcttc
    37381 gttatagccg tatgcttggc ttggtacatc gatcacaacg atgattggcc gtttcacttt
    37441 tttgtcttta tcttcttcgt aaacgtgatc caaaatttgt cccatgcgga aaccttcaac
    37501 taaaccaact tcaccgtttc gtacacggta catcgggttt ttaggatcag gtacgattga
    37561 tgtcacgatg gcttcttcta tgaatttact tgatttacca taatacacag tactaataga
    37621 agaagttgga tttttcatgc cagttagcgc cgcaaaccaa tcgtaaccaa cacttgctgc
    37681 agctggttca tttccttctg ctacttctgg taagtcaatt gattttgctt gatggttagc
    37741 aattgcttca ttgtaatcgt caatggccat tggttttgaa agatctaaag aatcaagtaa
    37801 tgataaataa aatgctgagc tttcactgcg atgcgtgtct tttcgttcgt ccattgctgc
    37861 gatgacagca gctttgattg cttctacact atcagtcact aattcatcaa tgatttttga
    37921 ttctacccgt tgacgagatc ctaatgtatt ccaaatcaag tctttgtcac tagaatcaaa
    37981 ttctcgaacg cctgcttctt gttcaataac ttctggtcca ttcaaaccga ctctcgcttt
    38041 ttttgttgcg attaaataac tcattaatgc tgaagtaatc gacataccgc caaatgatcc
    38101 tacacgtcct ggaatcaaac cgatgactgg tacatattgt tttagagcaa tgattacatt
    38161 tccgatttct gaaatagata acagtccgta gtttgcttct tgcaaacgaa caccacctgt
    38221 atctagtaca atgactggaa agatctcatt tcctttttta ttttcttcta acacatgttc
    38281 aagtgcagca acgatttttg caccggaaac ttcaccgata ccgccacctt ggaattttcc
    38341 ttccatagcg attactactg cttgtttgcc attgatcgtg ccacggctga caacgatccc
    38401 gtcatcactt tcagggacga ttccttgtgc tggtaaactt ggcgcgatga tttgatcgaa
    38461 tggatcaatc aattcacgtc cattgtcaag tagagcgttc actcgttctc tggcgtttaa
    38521 ttctacaaaa ctatttttca tctgaaagtt cctccattgc ttgcgtcaat cgtaggtaca
    38581 caactcctgg tgtagcaccg aagtcattga tcacgatatc tgctgaaatt ggataacgat
    38641 caaaaaatct cgttaataca ttgttccata cttcgccgaa gccatcgctt cctgtacaaa
    38701 tttttacagt agattctgtt ttttctgttg gaaacatcaa gatttccaaa tctcctgaac
    38761 cgaccactcc tacatggatt ttcttattga tcggctgttt cgttggatat ttataagcta
    38821 attgttccat ggatctctcc cctttttgta aaacagagca tacacgttct gcttattcgt
    38881 ctttcttctt ccatctgggt aaatggcgac aaaaggtttt tctcgccaat tttatgacca
    38941 agttctgaat ttcattggtg gttcatacag gccatctgac cattcgatca aatcatcgat
    39001 ggtttgtgca gctaataatg aacgtttcgc ttcactacgt ttgatgccta gatcttctgg
    39061 taatttcacg atgccggctg cacgcaagct atctagtgat tctttcgtgc tagtcattcc
    39121 aagaggtgta acaccgccaa ttgctgcaat tgctgcttgt cgttcttctt ttgtttttgc
    39181 tttgtacaaa taagcaattc cttcttctgt cacgatatgt gtagtatctt cgctataaat
    39241 catcactggt gcattggcta agcctgcttg tttttgaaca gctagtgcat ctaattcttc
    39301 gacaaagact ggttttttat ttgaaccata tgtttcaacc atttgaacaa ctaatttttg
    39361 gcctttccct aatggatcat cattgtcgcg aagtgattgc catgctggtg tagaatgacg
    39421 acgaccgcca ggattatgtc ccatgttagg cgcaccgcca aaaccagata aacgtccttt
    39481 tgttacggta gaagagtttc cttcgtaatc taattgcaag gttgaaccga taaacatatc
    39541 gatggcatat tgtcctgcag cttgtgataa tgttcggtta ctgcgcatcg tgccgtcttt
    39601 accgacaaaa aagacatctg ggcgagcagc aatatatttt tccataccaa cttcgccacc
    39661 aaaactatgg atgctttcaa cccaacctga ttcgatggct ggaatcatcg ttggatgtgg
    39721 gttcaatacc cagttcttcg cgattttccc ttttaaccct agtgattcac cataagttgg
    39781 cagtaacaat tcgatagctg ctgtattgaa tccaataccg tgattcaaag attgtacgcc
    39841 gtgttctgcg tagattcctt tgattgccat cattgccatt agaatttgca attctgtgat
    39901 gttttgagga tctcgtgtga ataaagcttc taattgataa ggtttatctg ctttcacgac
    39961 aacgtctacc cagtcaccag ggatatctac acgtggtaaa gaatcgacaa cttcgttaac
    40021 ttgaacgatc acgataccat ctttgaaagc tgcagcttcg atgattgttg gtgtttcttc
    40081 cgtattgaaa cctgtgtaca agttgccgtc tttatctgct ttatctgcag ctactaaaac
    40141 aacatttgga atcaaatcga tgaataagcg tccataaagt tccaaataag tatggatatc
    40201 acctaatttc attgtgccgt cttcaatcat ttgggcgaca cgcaagcttt gtgcaccagc
    40261 ataagagaaa tcgatcttgc ttgcgattcc tttttcaaaa atattcaaat gttctggtcg
    40321 tgagatacta gacatgatca aatgcagatc attcactttt tctgcatcta ctttttcaag
    40381 actttctgaa agaaaacttg cttgtttttg attgtcgcct tctaatacaa ctttgtcgcc
    40441 gggagcaatc agtttttcca ttacttctac gattttttca gtttctacat acttgccatt
    40501 gagcaagttg ctgatttttt ccaatcgctg cgcttttttt tcgcgtttag aagcccagtt
    40561 gcggtttggt ttttccataa ttagtcatct ccatttttga tatagaaacg tggcgtcatc
    40621 aataccatgt tggtctagat tgatcgtacg gatcccttta tctacaccaa attttgtatg
    40681 aatcagcttt gtcaaagtat tgccgggggg aaattcaatc gtcagatcca ttcctaattc
    40741 ttttgccaag tccatcattt ggttccactg cacagggaaa gctaggtttt ccaacaaatc
    40801 tttacgaatc tcttctgcgt cctgagtggc tcttccatcc gtatttttta tatacaaaca
    40861 tctagcatca gatagatgga cagactgcat atacggcttg aatttcttga ccgtatcatt
    40921 catcaagaga cagtgagatg gcacagggac tttcagcagc ttcgctgttt gcgcttggtg
    40981 ccgtttggct tctgccaacg taacggataa accttcgata ctcccagata acgtatgttg
    41041 catcggacaa ttttcatttg acagataaac tggatggtca ggatcaaatg tttttgctac
    41101 aatttcctca gcttcgcttc tcgtcacacc tactactacg cccattgcat aaccgcttgg
    41161 ataagcttct ttcattaaga ctgcgcggtg atagactagt tccgctgcat tctcaaatct
    41221 caaagatcga caagcaacag ctgcacaaaa agcaccaatc gaatgtcctg ctgcaatatc
    41281 aggctgaata ccgttttttt ccatttcatc taaataaaat actgcttttg tcagtaatgc
    41341 tagctggata aaaaccgtat cttgatagtt ttcttcttta tcaactaacc gataccccgt
    41401 cacattttct aaacgttcca tgtacatttc agggatatcc gctaacatcc ctaacttctg
    41461 actgccttgt ccaggaaata agaaacctgt actcatgagc ctcgcctcct cagcacttac
    41521 tttttccact gctatcttag tcaatttctg ctataataaa aaatgccaaa aaaagaaaaa
    41581 accataacaa aaaggtatgg atgggatgga taattatgaa attgcttcat ctgcagtatt
    41641 ttaaaaaagt attcgaaaca aaaaatgtta cccaagcagc caaagaatta tttatttctc
    41701 aacctgcctt gagccgtgcg atcaaacatt tggaaaacga attaggtgtc cctttgtttt
    41761 accatagcgg cagaaacatt gagacgaccg tttatgcaga agaattttat ccatatgcag
    41821 tcaagacatt agacacatta gaagaaggaa tcaaattatt gtcttcggtc aatgagaaga
    41881 tcgttacttc cgtcatattg tatttagagg tagcctctgt ttctattccg aacttggtcc
    41941 gaatttttat ggaaaagcat cctgatatcc aattaacgat catgcaacat catttaccag
    42001 atgatattac cgatcctgta ctgtacatca cttcggagat gaagcctggc atgaccaata
    42061 ttcctatcat gaaagaacct gtttatgtcg ccattcctaa gaaacatcct cttgctaaaa
    42121 aagaacatct gcaattagaa gatatccaac gcacccccat cttgatgctg tcaaagagca
    42181 acgccttccg tcatacactg gatgcagcac ttgaagcgaa aagtatcgaa ttgacgatca
    42241 gttcgacgac agatgatcct gcgacattac gttctattgt caagcaagga ttagctgtta
    42301 gctttttccc aaaagtttct tggtcttacg ataaaaatga tcctttcgtc ttgcgggaaa
    42361 tcaaagattt gcccttgaca cgaacaattt atttatcttc tagcttcaca gaagatcatc
    42421 ctttgatcaa aatgattgca aaaacgatgc gggaatttta tttaggaaaa taaaaaaaaa
    42481 agagctggga caaaattctt gtcgcagctc ttttttcgaa caaacggtgt tccagaagta
    42541 atttcttaga aaataagccg aaatttccca aaaatttgag aagcaatttt cgtaaattcc
    42601 ttcttatttc ttgaagctaa acatttctgt cacaacctct tctctattgt cctagacatt
    42661 cattgcgttg atttaaaact tccatcaatc aaatctgcta gttcatctag cttttctatt
    42721 ttacgtagat caatagagat ccaatgttct ttattcatgt gataggctgg aaaatacccc
    42781 tcttttcctc gcaaaattcc gccaagttca ggatcgacct tcaaatcaag aatattgatg
    42841 atcctatggt cacttgcatc atcatagatt ttttcttctg gaacagacat caggacccca
    42901 aaccattttt tgcttttttg atgtctgaat acgcaataat caggaaattt tttaaaaata
    42961 tattctggag acaaattgta tgtttcattg atgtgatcga ttagttcttt tttcgttagc
    43021 atctgcttcc atctctttct tattgattcg ctttattcaa agataccacg taagaataga
    43081 aaaatgaata caagtagttg ctaatcaaat acttcgatat ttttcttttt tatcaactct
    43141 taaatgttca atccgcctgt gttggtcttg ctaccatcta ctattaaaat gtttattatt
    43201 gtttgcccac ctattttttg attttagtgg tatcattttt caagttcatg atgaaattct
    43261 tatcatcagt aaatgctcca gaaaaataaa caatgattga aaaatcagtt gaaacttgac
    43321 caccgtttat tttctccgta ttcttagaat agaagggagt ctgattgatg tattcactaa
    43381 tgaaaaaaat cattaccgaa aaagatattc gacgtcatgt ttctttagta gaacaattgc
    43441 tgaatcatac aaagataacc gtaaatgaat tagctgaaat catagggaca accgaacgaa
    43501 cgattttttc tgaccttcaa tctattcgct ctcagctgcc cgaaggctgg gacatcatca
    43561 gcgatcaagc aggaatcagt ttgcaaaacc agcaaaattt attaacaaat gacctttggg
    43621 agattttttt caaacaatca gtcagtgtcg aactgctgaa aaatctgctt tttacaaaaa
    43681 aagttgctgt ccctgatttt ctagccgacc atgggctttc ttatgggaca ttgaaaagac
    43741 atgtgactaa aatcaatcag cgtttagcaa gttacgactt gcagatcgat ttgacgaaat
    43801 atacagcttg tattcttgga aaagagcgtg cgattcgtac tttttatcat cgattgctga
    43861 ttcctttcac tcataacaac tattttttcg aggattattc gatccatgaa tcccattatt
    43921 ttcaattttt aagaaacctc agtcaaactg aactagcggt tgaaacagaa gagattttcg
    43981 gaacatgttg gttctttatc aatacgatcc gtataaaagc gaattgccga ttagattctt
    44041 ctatccatac caacagcaca ctctcctccc tgtatgactc tgcattgaaa aaattgtatt
    44101 taacggaagg tatttatctt aaagacactg agctttcttt tgcttctttc tgctttctgg
    44161 aaagttggaa ttacaacaat aactatggac aagaaaccgc tcgctgcctg catcattctc
    44221 catttttaga ggtcctcgaa acgtttgtag aggaactagc ttcagagctg tctcttgatc
    44281 aactaaaaaa aacatctctg actgataact tggctctcct catattaaaa tatcatgaat
    44341 cgccgatatt gattgaagca ttggaccgtc aataccatcg ttttttagaa acatatgatc
    44401 agcaatatgc acacttgtat caacagaaag agaatttact gaacgatcta aaaaaagaaa
    44461 tagaaatcaa tgaaccaaat tactttctcc aactcttgtc tctactgatc caacaaacga
    44521 ttttttctat caaaccgaat ttgttcaatg tctattttat tttccaagga gaaccttcat
    44581 ggaaggcgtt cttgcagcag gaattaaaag attacttagg aaaacgtgtc tgcttcttac
    44641 ctatcgaact aaccgatctt tccactattt cttttgaaaa aacagatatc ttgatttcca
    44701 atttccctct agattctcta gatatcccta ttgtatacat ttcttctatt ccaacgaaaa
    44761 atgagctcaa tcgtttgact gaactcactt tcaaaacttt tttatagtaa cggctagcga
    44821 ctgtattctc tagctgtttt ttcttttttg cgaaacagtt tgttcatgat ccctttttct
    44881 atttctacaa ctaagaatag aagaatcaca tttgctccaa tcaataactg ttgtgtcatc
    44941 gttaaactta ctgtaccgat cagctgctgg ccgactggta gaaaaataac catcccttgc
    45001 aataaagcaa ggatacctaa tgaaagaaaa agcgctttgt tttgcaaaag accttggttc
    45061 aatgacggct tggacaactc tctgcaattg atcatgtaaa cagcttgagc taagacgata
    45121 ttttgcagaa gaatggtttg ctgtaagctt tgcccttcaa actgcatagc caataaatag
    45181 gcaggaacca tgatcagtag ggagacatag atgatccgaa acaaatgata accagatagg
    45241 attccttgct ttggatctct tggtgggcgc ttcatgattt ctggatctgg cgactcgaac
    45301 cctagtgcat aagataaagt gattgtcgtt accatattga cccataaaat ctgtactggg
    45361 gacaaaggta gcgggcgatt cagcataagt gcccaaatca agatcaatcc ttgagctaat
    45421 gcagttggca agaagaaatt gatggttttt tgcaaattat ccaaaatccg ccggccctct
    45481 tttacggctt ttgcaatcgt atggaaatta tcatctccta aaaccatatc agctgcctgt
    45541 ttgcttactt cactaccttt gattcccata gcaattccta catcggatct tttcaaagca
    45601 ggtgcatcat ttacgccgtc tccggtcatt ccgacgattt ctccattttt ctgcaaagct
    45661 ttaacaattc gcagtttatg ttctggtgtc gttcgagcaa agacatcgac tattggcact
    45721 tgctgaatca actcttcatc agacataaga tccaaatcga tcccttctaa aactttggca
    45781 gtatgtttta agccaatttg ttccgcaatc gcttttgctg tgtctttgtg atcccctgta
    45841 atcattttca ctgagatccc tgcttgttgg cattccttta cagctttgac tgcactttct
    45901 tttggcggat cgatcaatcc ggcgatccct gcaaaagtta attgggttaa agtctcatgc
    45961 gtgagttcct gtttcgaatc tactttttta taagcaaatc ctaaaacacg ctgacctttt
    46021 ctcgcaaatt ctgcagctgt ctgactccat gcttcttgtt ctgcagaaga aagagttgat
    46081 ttagcaaata atacttctgg tgcacctttg acgtaaatca ccgctccttt ttcctccgca
    46141 tgcatcgttg ccatatattt gtattcagaa ctgaacggaa tctttgcttc tatttttttc
    46201 aaaggtagat tggctgtttc tgtgtgtcga atcagcgcaa cttctgtcgg attgccactt
    46261 aaattttctg ttttctgttc ttcttttgtt tttaattcct gacagttttt cataatggac
    46321 aacacgcagg cttcttcttt tgcagctaca tccacaactg tcatttcatt ttttgtcaat
    46381 gttcctgtct tatctgaaca gatcactgtc atagaaccta atgtttctac cgaaggaagt
    46441 cctttgatga tcgccttttc ttttgccatt tcatgtactc caacggatag aatcatggtc
    46501 aaaacagctg gcaatccttc gggtaccatt gacaccacca aagcaatcgt agccgaaaga
    46561 aggatatgcc aatccatacc atatcgaaat gtcgtaaaaa taactaagaa taggctcaaa
    46621 caaagaatgc cttgaaaaat ttgtttgttt aatcggttga tttttttaat cagaggcgtc
    46681 gtttgttgtt cgaccgattg caatgcttga ttgattttac cgatttctgt cgtgtttcct
    46741 gtttctacaa cgacaccgac tgcagaacca gattgtacaa gcgttcctga aaaacccata
    46801 tttttctggt cgccgatcga ttcttcttct gtcaaaacgg catgactttt ttcagatgct
    46861 tctgattctc ccgtcaaaat cgattcttcc actaataaat catgtgcttc gattaatcgg
    46921 atatctgctg gaacgacatc tccggcacgt aagacaatga catctccttt taccagttct
    46981 ttcgcaggga ttttttcttg catcccgttt gatagtacta tcgcttcttg tcccatcagc
    47041 tgcttcaaac cattcaatga ttcttccgct tttctttctt gccagtaact aacgagtcca
    47101 ttgacgatca ctacgaataa aataatactt ccttcaatat attcactaga agcaaatttc
    47161 aaaaaagagg caatgatcaa cacgatcatc agcagatcag taaaatgctt tgctattttt
    47221 ttccatcgtt ttgtctgctg ccttgctttt atttcattat atccttgatc agtcaggcgt
    47281 ttctgtcgtt cttcttgcga caagccttct gttgatgttt gatagttttt caatacatct
    47341 tctaccgtca atctgtacca gttcatccta tcccctcctg tttaattgat actgttatta
    47401 tacgcaggga agttttttca ggcgtatcga ttatttcagg ggggtctgaa ataattcgtt
    47461 ttgcaaaatg tatctgggcc taactgatat ttctattgct tattctttta tatacaaaat
    47521 ttaagaatac aaaccagata accaataaaa aaggaataaa gcgtgtcgtg ctttcgaata
    47581 acaagctgaa agcaattgaa gtaaaaaaca ccaacaaagc aagatcttta tatctttgtg
    47641 tagtgttccg tttcttttct ttaataaatt gaacatgtgc acacaagatc gaagaccaca
    47701 caaatagaaa acaactcgtt gtcatgcctg ccaaaagctg aaaaaacaat cgactatcag
    47761 ctacaaaaaa atgtagacaa agaccggcaa aaaataacat tcctgtaatt aaaataccgt
    47821 gtaaaggtac gcctttctta gaaagcgcac aaaaccaatg gggcaaactt ttttcttttg
    47881 ctgtttgata taaaatcctg ctagtactgt agactgcact atttgctgag gacaaagaag
    47941 caaccaaaat cattagattg acaattgctg ctccgccttg gcaatggatc acttgcagca
    48001 tagtcactaa aggactttct tttaaatcta aacaatgcca tggaacaaca aataaaatcg
    48061 acaaaatcgt tcctatatag aaaaagagaa tacgccaagg cagacagttg attgcttttt
    48121 tcaaattctt ttgcggatca cttgtctcgc caacggttaa gccgatcatt tcaattccag
    48181 caaaactgaa taaaaccata ggaaatgaga ctaccattcc agcaaagcca aacggtagta
    48241 attcatcgat ctgtccaaaa ttttctttga ttccgataga ctgggtatga aaatctgaga
    48301 cactcaatcc tatacccaat aagataaaaa ggaaaatccc caagatcttg atgattgaga
    48361 aaaaagattc caattcacca aaccacttga ctgcaacaag gttgactgcc attaagatca
    48421 ttagtgcgct acctccgctt atcaaagctg gtacatgtac aaaccagaaa tggatataca
    48481 atcctattgc tgttaattcc gcaagtccag ctccaagcca gcaaagccaa taagtccaat
    48541 tgacaaaaaa tgcccacttt ttgcctaaat aatagtcaac gaaatcactg atagagttaa
    48601 atgtactatt tgctaaaaat aaatcaccca tggcccgcat caaaccataa atagccaaac
    48661 cgcatatcaa ataaacgaga ataacagctg gccctgctga tgcaattgcc tttgctgaac
    48721 ctaaaaataa tcctgttcca attgcgccac ctaatgcgat taactggata tttcgattag
    48781 ttagagtccg tttcattgcc ggtttttcca tcaagttcac ctttttcttt ttcaagcagc
    48841 atggcgactt cctgttccca agaaggtttc tttatttttt caatacagag tggtactaca
    48901 aacaaaatga cactgatggc tgtgactgca attagattga tccatgttcc agctttcatc
    48961 gtcagtccga cagaaaatcc gattgccgca aataaaacaa taacgacagt ccaaactcca
    49021 agattgctct ttttctcttt ttttccaatt ctaaatggtc gattcaactt tgcatgtgta
    49081 taacgcaatc tcaaaactgc gatccccatg atgacataga cgatacagta gataacagta
    49141 gttgaattta cgagcatcaa aaatgcttga ttgacgccag gaatcagcaa ataaaaaagt
    49201 gcaaagattg agataatgac agactgagtc aaaataacgg atttggatac gtcaaattga
    49261 ttcgtcttcc aaaaattgaa ttttggtgga tacgcacctt ttctagcact ttctgtcact
    49321 gtctttgctg gtcctgaggc ccaagcagat aattggactg ctacaccaat gaagacaagt
    49381 aaactaaaca gattcacaat aagatgtggc aaaccaagaa tttggcaata aatcgaaata
    49441 gattgcgcga tattattcaa ttccatttgt ccttttggta cgacatttgc tacaactaaa
    49501 gcattcaaaa tattgacgaa aaatataaaa attaacgcgg caaatactcc gcgaatatac
    49561 gttttaactg gattttccaa tcgagtgata tacacagagg acatttcgat accagtaaaa
    49621 ataaacatga tcggcgcaaa ataaacaaat gaggatgcag tcgtagtatc tggtattaat
    49681 ttgtcccatg aaaaactgcc taatgttccg gttggtacga tcccaacttt gatccacgca
    49741 gcaaacccta ataagagcat catcgtcaaa ggcacgtaca atcctaacca aacaccgact
    49801 tttccgccga tttttgccat atcaaatttc aaattcaaaa aagtgatgat ccaataaacg
    49861 actaaaatga caactaatgt aaatttgctg ttcaaaccaa gcggcacatt atcgataata
    49921 ttaccaaaca acggagcgag tgctgaagcg accatcacca tcccaggaaa catttggacc
    49981 cataacagcc atgagacaac gaacccccat ttttttccta gagcattcga tacccaaagt
    50041 tctggaccac ctttttgggg aaacattcca gcaaattcac cagagatcaa tgagattgga
    50101 aaagcgtaca ataatactgc aaccagcatg taaaaaatca tcgtccagct cgttgctgct
    50161 acatctggaa tatttcgtac acttgctaca agggcgcagg tcattccgat aaaggtcatg
    50221 gtcgtgattt gctttgatga accagtatct ttcatcttta ttccttcttt caatagcgac
    50281 atatgttttt gttttctttt atttttgttc aagaacagaa atgacaaggt ttcatcgatt
    50341 cagtacaaaa cccagtatca ccgaaacatc cattgaacga tcttagtgaa ctgggtttga
    50401 tacatgaaac gaacgatcca acttatagaa cgttttataa aaaagaatat cttggcgagg
    50461 aatatatgaa aaacaggggg aaatacaatt gttttgatga agcaaataat tgtctgttct
    50521 ccatatgtct cttgttattc tttcacacaa aaatcagttc tattggatga tttcgtttag
    50581 tttcttttcc attgctttgg cgattttttt tgtataaaaa ttgaaaattc gatcatcatt
    50641 caaataagga gtcatgatag ccgcacgtag cagagtgatt ttctgttctt ttttccattc
    50701 ttctttcgtt agacccattc tttcaacaaa acgaatcgga gaatcaccgt aatcttcttg
    50761 tgtaaatgta gtatgagaag taataaaccg ttcaccataa acatcgccat ccatataaga
    50821 agaccgatcg aacatttttt catttaattc attgatagcg ttcaaatctg tacagccttg
    50881 ttctttgaat acccaattga ccatattaaa atcaggatga ttcaatggat agacttcgat
    50941 tgtttttcct ttaacagtaa aagtcagatg atcgagaaat tctctgaatc tttgagctgc
    51001 ctcgattgaa gccccaatca attgaccata tccggtcaca ttcaatggca atacacgatg
    51061 tgctgtccag actgcggcgg cggttgctcc agcttttgat ccttccaaaa tataagctcc
    51121 taacatatct ggagccttta ctgatttttc aaacacatat ggcgcgaaat aagaaatgat
    51181 atttctcata tttttatgtt tgattacgat tcctccagca gcataaggga catagcccat
    51241 cttatgagga tctatcgtga cagaatccgc ttctgaaata gctctgaacc cttcatatac
    51301 ctctttgtca atcgttacgc agtggtggaa aacatgatgt tcttcaaaga attcagctaa
    51361 ggaagcatac ggcacaaatt ctcctgcttc gtttagaaac agtgaacgcg catagccgcc
    51421 atatgcggca tcaacatgta aatagaaata gatcccttca tcttttaacc tttctcttaa
    51481 ttggacgatt ttatctacac tgtctacttg accttcttct gtcgttccta cgacagcaac
    51541 cacacctaga attgggattt tttgtccagc taattcacga attgtttttt ccaaagcatt
    51601 aatatccatt cgataatcct cttgaactgg gatagcaacc atctgatcta agccgactcc
    51661 gcaaatatct aacgccttca tccaagaata gtgttttgtt tgagggacca gccatttccc
    51721 taatctttgg atattttttc cgctacggga agaagctgct ttgacctcat ccatttcttc
    51781 gtccgtaaag ctttcggtca tttccaaaat ttcttcaaca gataaattca aaagttccca
    51841 ctcagacatt tttttcactt tttcaggcag cacttccttg acagctaaag gaatcgattt
    51901 gatgcaacgg gcgtaccata atccttcaag attagcaata gaaccatctg ctgcaatgtg
    51961 tccccatccg tctgtcatat tgaataatga agcaaaatcc tgtcctactt ctgcttccat
    52021 ttgcgaagtt gccatagaag attccaatgc aacattattt gggttccaca gcatcgcata
    52081 gttgtaagct aataaagcag gcatcaatgt ttcagcattc atttgtcccc agtaacggcc
    52141 tgctgaatgc catgggatcg atcctgcgcg taatcgctga ctgacttcat ccaagacctc
    52201 tatcatatgg tcgcgggtgg ctagataatc tggagtgagc ttatctcctt cgctaattgc
    52261 aggcatatct gaaggaatat aattttctct ccatccaaga tgttcatcta ccatcttgtt
    52321 caacaacatt ttataaactg gtccattttc agctttgtcg cctataaaga cggccttgat
    52381 atccatatct ttcatttttc ttccctccat attttgttgg tcaatgtctt gcagatacat
    52441 gtgtttatct gtaacacctc ttcactatag cgccaaaaat gagaattttt tttttataac
    52501 atcaaatcca cgtagatttt tacaaacaaa aataaataaa gtcggatttt tctgataatt
    52561 caacgcaaaa aagcgaacaa tttttcaata aatcagtctt ctaaaataga taacctaaaa
    52621 aaagcgaaca aacaaataaa atgactgtcc ttcattcact aaacatattt ttattttttt
    52681 atattgttaa aaattcgcaa tttatttttt aatagaatta tctgttttcc aaattttata
    52741 acataaaaaa ccttttttaa cggcatttat atgctttttc agaaattaac tgatgatttc
    52801 aaaaagaaaa catgtcataa tatttcccat attgtgcaaa aaaatacaag gcggaggctc
    52861 tactatgatt gaagaatata ttgagaaaaa tattttaaga cagctgtttt tatgtgggca
    52921 attctatgta aataaagaag tcaatctaga aaagctctcc aatctcctgc atgtttgcaa
    52981 aaccacttta ctcaatgata tcaataacat aaaaaaagaa tttgaagaac agatcgctta
    53041 tacacatcgg gaaaaggacc gttacacttt atatttttca gaacatatac ctcgatgcaa
    53101 gatcatgcag caattgtctc aaaattcgtt gtttttaaaa acttgtctgc tttatttgga
    53161 agaagatgaa cctgattatc ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc
    53221 ttattcatta aaaaaacaag tattagctta ttttaatgac tgcgggatcg aaatcgaccg
    53281 ctactcccct cgattcactg aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg
    53341 tttaggcggt tttaattctt gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt
    53401 cattgaatcc gtaacggaaa atagtggcag attttatgat aaagaaaata aagaaatctt
    53461 atcgattggt tttgctatca gtttcctacg ccagcaagtg tgtgctgtga caatagattc
    53521 aaaatttatc gaagaaatca aaaaacgtcc tatttacaat tatgtcgaat cagcatggga
    53581 aaacactgat ttccaaacct attataaaaa agaagaattc gcctttattt tgacactatt
    53641 caatctttgt aattatggtt tccattctta tcagttgatt gcggaagact ttcaacagct
    53701 acaccaagtg tttattgaca atactcccga aataaaagaa ctagttgcta cctttgaatc
    53761 tcacttcaat caagaattat tcggaaacca accgttcgaa cgtgcgctga ttcatctgat
    53821 gcgttcggct tgggataact atcaattatt catgccagaa aaattttatc ttttaaatga
    53881 ggaacagaca aatttatata aagaagtcca gacaattttt tccagctggt ccagccagtt
    53941 accttatgat ctacggttga atcctaattg catgcgggct tttgtgatcg aattgagcgg
    54001 aattctccgt ctgacaaaag aacatctgac tatctatatc gtcacaaatt cggatgttca
    54061 ttatctgatt tatcgggaag ccttggaagc tgtaactact ttcgattttc aagttgcacc
    54121 aacaatttat tcaagtatat ctgatataaa gaagtatgcg cagcagtcct caaaccgggt
    54181 attatgtgaa cgtacattat atacaccaga tgcggtccaa tatgaaaata ttattcctat
    54241 ttcgatcaat acgatcgatc gagctatcat ttcagctgtg caaaacaaat aaggggctgt
    54301 gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc ccatttctgt aacaatctct
    54361 tgatacacgt caggctcaca acctcactta ttttggacat ctacgggtcc gatctcttta
    54421 tttaaaaaat aagaaatgat ggttcggata actaccaacg ccgctagttg gaaaatatcc
    54481 tgcaaggtag gtttgataat cgattcgatg atcccagctg aaacaagaat ttccaaactt
    54541 agaagtatat agcctcccaa catcttttta atagcattgt tctcgctgac tcggtcgctt
    54601 tgcacttttg tatggacaaa taaatatttc aaaaaatcct tgattgatag acaaacaccc
    54661 caaaccaata aacagatagc aaagatatca agtcccagta caagtttttc gaaatatgga
    54721 agaagaaact gcatcaactc tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt
    54781 cttctatttc ctgattcaag aaataagaaa tgactgttcg aatgataata ataagtgcaa
    54841 gcttcaaaat atctgtccaa gtcggcttga taactgactc gatgatatca gcaacgacta
    54901 gtacttccaa gcttaacaaa atataacttg ctagcagttt tcttgtatct gcattacgag
    54961 tattacgttc tgcatagttt ttcttgacat gagaccaatc aaaaagtttg aatagaacaa
    55021 gaacgatccc gacaattaag ataccgatag aacaaatatt taaaatagca atgatccatt
    55081 ctagaaattc aattaaattt gagcgaaaca tatacgctac ctactttctt ttaatttgtt
    55141 cctattcttt tattgtaaaa gaaagttacc ttgttcgtct atttaaaagt tgttctaatg
    55201 ataacggttc gcttaaattt ttctttttca ctctcccatt tttctccctc catccataaa
    55261 tcgtggaagt atccaaaaca acgtagcaag ttaaaataag gcttagtccg tcatcagctt
    55321 ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa tttctaaaaa tagaaccata
    55381 cacctcctca aatcttttct tcttacctac cctattttta tttacaggaa aatcagacac
    55441 ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct atcatttggc attctaataa
    55501 aaacgaacta tccttggata tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa
    55561 attaatcaaa gtcggcatcg gtttaggttt gcttgccctt ggcgctgcat atctaggaaa
    55621 aaagacagga ctattcgaag atgacagtca tttatatgat gaattcgaat caatctagca
    55681 tcctatttag ttgaatcact acggacatac ttggaagaca ttggaggaat cacaatgaga
    55741 aaaagtaaag caataatgat tggtgccggt attgccaata tggcagcggc tgtttatttg
    55801 attcaagaag gcaaatggaa aggggatcag atcacctttt attcattgga tgatcacggt
    55861 tcaaatgatg gtgcaccgac cgacactgta gtggatgaat attggaataa aaaccatcca
    55921 ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca tgcttaatta ccgtacctat
    55981 gtagatttga tggatctgtt aagtcgaata ccttctgtta ctgagccagg gatgactgct
    56041 gaagaagata cgagagattt tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg
    56101 gaaggtggca aaggaatcat tgatggcgga aaactgggat tcaataataa agaccgtatt
    56161 cttctgacta aactgattgc tatgcctgat tcagaagaag aaaaactaga caatatcact
    56221 atcgaagaat atttcaaaga tgatccacac ttttttgaaa caaacttctg gtttatgtgg
    56281 gaaacaacat ttgctttcag aacacgtagt tctgctcaag aattgcgtcg ctatatgcat
    56341 caaatgattt atgaattcac tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac
    56401 aatcaatacg aaagtattat gcttcctttg atcgagtact tgaaagaaca aggatgcaaa
    56461 atcattttga accgccgtgt cactgactgg gaattcaaag aaacaccgat gcaagatgaa
    56521 atcactgtga ctggtttgaa aattaccaat acactgactc aagaagaaga atacgttgtg
    56581 gtcgatgaag atacagcagt tattttcact aatgggtcca ttacagattc agctacttta
    56641 ggagattacg aaacaccagc tgtagaaaat atggattatg gtgcagcttc tagtctctgg
    56701 aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg acaaattctt tgctgatcga
    56761 gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa accatttgtt gttgaatgaa
    56821 atcacccgta tcacgactca agaaccagga aatgccttga attcatttat ttcaaccaca
    56881 ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta tcgtcgtgca tcatcaaccg
    56941 cattttacgt ctcagaaacc aaatgaaaca gttatttggg gttatttcct ttatccaaga
    57001 cgacgtggcg aatttgtgga taaacagtat attaaaatga atggaaaaga aatgctagaa
    57061 gaactgattg gacaactttc aaaagtagat cctggaccag tgaatattcg tgaaaaagaa
    57121 acagaaattt tcgacagcgt aataaataat atccctgtct atatgcctta tgcttccgcg
    57181 ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat
    57241 ctggccttta caggagaatt tgtcgagcag ccttatcaga tgatctttac tgaacaaagt
    57301 gctgtacgtt ctggagaaat cgctgctttc cattttgcgg gtattcctat gtcccgtctt
    57361 gtcaaaacac cgcgttacga caaagatatt cctactctag ctcgtgccgc aaaaaaaatg
    57421 tttgaataat caagaggttg tgaaaaagct gccctgcatc aagtaataag aacaaccagg
    57481 ctaaaacagc ttcttatgtt tttgatttgg ttggacttat taccgcagat gcaagctttt
    57541 gaacaccgtt taccaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaccc
    57601 aagctaaaac tgcttcttat gtttttgact tggttggact tattaccgca gatgcaagct
    57661 tttgaacacc atttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca
    57721 gccaagctaa aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa
    57781 gcttttgaac accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga
    57841 aaaattttgt gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac
    57901 tgatcccttc tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt
    57961 ttttcaacgt ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg
    58021 attaccatta cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat
    58081 agaatgtttc atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa
    58141 atgaaattat ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc
    58201 caatgaaagc agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc
    58261 caaaaatatt agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct
    58321 ccgatctgca tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac
    58381 acgaaccaat gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc
    58441 aacgtgtggt tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca
    58501 tggaaagcca atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg
    58561 gcaagttgaa cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag
    58621 actctacttc atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat
    58681 cagatatttt gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata
    58741 cagttgtgat tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg
    58801 ctggagcaga gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta
    58861 attacaatgg ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt
    58921 atgaactggc taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag
    58981 agcctgtcaa agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat
    59041 tacagatggc atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata
    59101 tgactccagc ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc
    59161 atggacaggc accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac
    59221 tattcgatcc tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat
    59281 atgagatctt tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc
    59341 agtaaaaaat acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg
    59401 aaataggaaa agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa
    59461 atcgttacac attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg
    59521 gaagccttgc ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg
    59581 gtgctatttt cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg
    59641 acgaaaaaaa agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat
    59701 ctcatgcctc ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact
    59761 ttcttccttg accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc
    59821 tgtaattaag taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa
    59881 tatccgcttg gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt
    59941 tggtgtcaga tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat
    60001 ggtttctttt tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc
    60061 agtacgaata ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt
    60121 cgcgctttgt tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa
    60181 tgtttaaacc tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat
    60241 tagcaatgac aacaatagtg atgtaaaaaa aaaacggaaa acaatcactt gttctccgtt
    60301 ttttgattaa tcaagcatct aaagcttctt ttcttctttc ttggatcgta gctaccttga
    60361 cgaacggtag atagatcaaa atagaaagaa ccaaattgac agcggctagt acaccacctg
    60421 caatactttt tgttgccaat acaccgccaa taattggtgg agttacccat ggaggcataa
    60481 cggtagctgc tggcactaaa cctgtagaag tcgcaaagta agcaacacta actaagacca
    60541 ttggtgtcaa aataaatgga atgaacatga tcgggttcaa cacgataggt aacccaaaca
    60601 tcgttggttc attaatattg aagacacctg gcgcaatcga caagtttgtt acaaccatat
    60661 atggtttgtt tttacgtcct actaaataaa tagctaacag taaacctaaa gttgcacctg
    60721 ttcctcctaa gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac ggtgcgctat
    60781 gcccagctgc gattgcatta acgtttgctt caatggctgg cgcattgatt gtttgcatca
    60841 aaggatcgac catattcgct ccgtgtaaac caaagaacca taagaatggt gtgataaatg
    60901 caagcagtaa ggcagatgga taagagtttg caagtcccat aaatggttct tggaccactg
    60961 tatagaaaga acctacgata tcatcaacac cgaatgcagc aaaaattgca gcaactaaag
    61021 caaacgcaga gatagtgatc attgctggta gtaatgaagc aaatgctttt gcaactgccg
    61081 gtggcacacc atcaggcatt ttgatgatca atttttcatt tccgcttaaa cgtacaaaga
    61141 tttctgttga aataatccca acaataatag caataaacaa accgtttgag ctcataccta
    61201 atgctccgcc taaagcgaag aaagagccta aagaaatcac acctgcggaa acaccgtctt
    61261 ttcggtagcc gttagctaaa tgataagcaa gcaagaaagc aatcaagaca gaaagaatgg
    61321 caaatgtacc gttccaagca gcagccccga atcctttcca tgattcacca ccaaaaattg
    61381 agttcatcaa ttcttggaac ccgggaatcg gtaagttatt gatcattact gctaatgctc
    61441 ctaagatcat taacggcata ctcaccataa atgagtcccg aatcgcaact aaatgccgtt
    61501 gtgcaccaat ttttgatgct acaggtataa aatgtttctc cataaatgca acaaatttat
    61561 ccataaatct tccctctttt ctttactgta gattacgcgt tcaatcgacg atacagatca
    61621 atgatctctt ttgctaaatc tgtaaaagct atagatgtca tcagatgatc ttgactgtgg
    61681 accgtcaaaa gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag catctctgtt
    61741 tgagagtgat gtgcttggac taaagattcc tctgcatctt tgattttttc atctgctaaa
    61801 gtgaaatcac cttttttcgc agcagcaatc gcttccattg catcgctttt tgcatttccg
    61861 ccaaacatga tcagacccat gatggcttct agattttttt gatcttccat ttattgtatc
    61921 ctccgataat ttttattatt tgtttattag tttttctgct tggtcgagta ctttttcacc
    61981 attcatcatg ccatagtcag ccatattgat tacatctaaa ggaatgcctt taggtgctag
    62041 cttttcagca aattgatttt gcataaagcg tacttgcggt cctaaaagta aaacgtctac
    62101 atttttagat tctagttgat tatccgcatc tgaagcagat acagcaaaga tgtctgcttc
    62161 cattcctttt gcttctgctg ccttttgcat ttttgtgact aggagacttg tactcattcc
    62221 agctgaacat acaagcatga ttgttttctt ttccatttta aacacgttcc tttctatact
    62281 tacacttgta tataagcaag tagcgtgcca actttaaaag cgctttaaaa agttgatatc
    62341 atcacattta aggtctatac caagttgcat acacactaaa aaagaaagcg cacactaaac
    62401 acacaaatta atttttatta ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca
    62461 aaacttgtca caactttttt caaataaacg gtattcaaaa gcttgcatct gcggtaataa
    62521 gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg
    62581 caggacagct ttttcacaac ctctcaataa acggtgttca atcagctgac cctgggtatt
    62641 agctcaaaac gctggattca caacctcttt tatttcaaat aaccagtaaa tacaatgaac
    62701 atctacaaaa aacttctatt tatttataga tttcgtcagg caaatccaat tctctcttat
    62761 caatcaaagt ctgataccac gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca
    62821 attcgattcg aactaatcca tatcgattct taaatgcatt cattggtgac acacagtcag
    62881 tgaatgccca taacatatag ccttcgcaat ttgcgccttc ttctgttgct ttgatcaagt
    62941 aatgcaaatg ctgtccgata aattcgatac ggtaatcatc ttgcacgatt cctttctcat
    63001 ccatatactg ttcttcatat tctcttccca taccgttttc tgttatcagc caagggatat
    63061 tatgataatt ttcttttaga tacatggcta tatcatacat gattttcgga tagatttccc
    63121 atcccctgga attattcatt ttcttcccag gtaaatcaaa gacttcataa tatcgttcag
    63181 gatggaaagg tgtctcttca ttccattgat aacgaggggc gcgaacgcgt ttaggataat
    63241 attgattgat tccaaggaaa tctactgtat gatgctgaat cgtttctaaa tcctctgttg
    63301 caggatcgaa ataaatcgat tgtttttgac acaactccag gaactcttct gtgtagtatc
    63361 cttttaccat cggatcaaaa aatactcgat tataaaacaa atcatacata taagcagctt
    63421 tttggtcttc tggagaattg gatcgagcat agaccatttc aggatttaaa atacagccaa
    63481 tccttccacc ataatttccg ctgcggtaaa tctctactgc tttagccgtt gctaaaactt
    63541 tatgataatt ccacatcatc cacttttttg tgttttgttc atgcggccag cgaacagcat
    63601 ccaaatagca acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc
    63661 tatcaccgta acgatcaaag gcaatttgag catacttagc ataaaaatca actacttttc
    63721 ttgaagacca gccatcatat ttttccaaaa gaaaagctgg gacttcataa tgttccaaac
    63781 aaatcatcgg ttctacgtct gcttcgatca aagagtcgat catttcactt atatgctgag
    63841 catattcttc atctactata cagttctcat aatcagtaaa aaaacgagcc caattgatag
    63901 aagtccgata atgtgtcaga tggatggatt tcatcagatc cacatcctgc tggtattgtt
    63961 ccataaaatt agttgctacg gctgggccat acccattatg ccaaacaaaa tgttcatttt
    64021 tataccagct atctataaac gaatcctgtc cttctttttt tcctttccaa ccttccgttt
    64081 gccaagcaga agatgcagca ccaagaataa aattttttgg aatagtaatt ttcatgattt
    64141 cctcctgttt tttagatccc atcattttcc acacgatttg ctacttttac aaatggcaga
    64201 tagatcaaaa tagaaacaat aatacaaaca agttgcgtaa taactgcacc gatatttcct
    64261 gctgttgcca gataagcatt gacgatcggc ggcattggcc aagggaccat tacaactgct
    64321 ttcgccgcaa atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat aagaggcgtt
    64381 aagataaacg gaatagctaa aattggattc agcatgatcg gcataccaaa aataactggt
    64441 tcattgatgt tgaagatccc aggagcaagg gataacttgg tgatttgttt catatcttcc
    64501 cgttttccaa ctaacaatac tgcaatcagt aaaccgatcg tgactccaga cccgcccata
    64561 ctcatataca tatcccaaaa tggcatagta ataatgttcg gtacttcttt tccagcttca
    64621 aaagcttccg tatttacagc aattgccgct aaaagcaacg gttcccgtat cggcttcacc
    64681 atttgatttc catggatacc aatcacccaa aagacttgcg caataaacat caatagcaaa
    64741 atacccggta acccttgtac gacaccttct aatgggcgtt gtacgacatt gtaaatgata
    64801 tcataaaggt acattcctgt aagttctttg atgataaatc ccgcagccgc gatacccgat
    64861 acagtcagaa tcgtcgggaa taaagcagaa aaactagcag aaacattcgt tggcacggta
    64921 tcaggcattc tcagctgtag tttcttctgt ttcctcaacc agcaataaag ttcaatagaa
    64981 aggatggaga ccaacattcc caaaaataac cctttcgtat ctgtatattg tttagccaat
    65041 acgttttcta ctataacagt ctgattttca ttcactaatt cataaacagt tggattaaca
    65101 gataaataag acatcactgc gatgatacca gggaatacac catccacttt attcagcttg
    65161 gctaattcga tccctatcag aaaaacagca tagatggtca agaaacttag tgtgacatag
    65221 ctgatggcag atgcgatagg ctttaattct tctaaaaaac gcaagaaact tatctgtgct
    65281 aagccatttt ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc acccgtaata
    65341 attaccggaa tcaatgcagc aaatgcgttt tttatcacca tgatatacct taaattattg
    65401 attctcgttg ccacgttccc taaaacattg gctaatttat ccataaaact cattccagat
    65461 cccctcattc ctgcttcttc tccttgacgt catctctttt tgtctattag tcagcgctta
    65521 catttcgatg aacagaaact atttactcct cttttttacc aaatataaaa caaaaattca
    65581 tctgatgtat ttatcataaa atacatcaga tgaactgtca attagtttct ttttctccta
    65641 ttattttctg taattctctc cgatttattg ccatatggac agtcattgca tcaaacgcag
    65701 caagtgcatt ccgcctttcg atggcctgaa cgatctctgt atgaatatcg atcgtctcga
    65761 ttttcagatg ctggatatca taatttttat taaacaaagc aattgattca ttaatgattg
    65821 ggataatatg cagcaaggct acgtttccgc tagcttctgc aatcatcgta tgaaaatcga
    65881 tgtctaattg aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc ttttgcgtta
    65941 attttttcat ttggacgatt tgattcttcg ttgcatattc cgctgccaaa cctgccatct
    66001 taggctccaa taaatatctc agttcaaata agtctcgtat catccgatcc tgatcttcaa
    66061 tcaaagaaaa accgagtggg tcattggcaa tccctcgctt atcactgaca aatgtccccg
    66121 ctccttgccg aacttcaagg atattcctgg aagctagagc ccgcacagct tctcgcaccg
    66181 tgcttcgccc cacctctaaa taatcggaca attcatattc attcggcagt ttatctccta
    66241 cttgcaactg catttccttt aaatatctaa ggatcttgtc agtagttact tctactaggg
    66301 ttttattttc ttccaaacca catacctcct tttcatctga tgtatttttt cgtaattatt
    66361 ataacacgtt tgaatcatct gatgattttt tgtatacaaa attgattaat ccgttcatag
    66421 acatacaaac gaagaagata tttaccataa aaaagactag gataaaattc actcctagcc
    66481 ttcgatcatt tttattttga acgacaaaaa atatgatgtt caataacaga cgatcttcat
    66541 tcgattcttg atgtcatctt ttgttttttt taatatatgg ataaaaagtt tgattacatc
    66601 gtttctattc tagaatttgg tcgattctca attcttcttc tggtgagaaa tctaaattat
    66661 ccatcattcg aacactttct tgtaactgac ttaaccgact tgctccgacc agtacacttg
    66721 taacagattt ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat tgtcctcgtg
    66781 atacggcgat tgtttgcagt gctttgattt tttccagagt tgatccaact tgttcttctg
    66841 aaaggaatgg tatttcctta cgatgtgctc tagaatcttc aggaatacca tgtagataac
    66901 gatttgtcag caacccttga gcaagcggac tgaacgtgat tgcccctagc ccttcttctt
    66961 ctaatactgg agatagatcg tcttctagta gtgcacgaga aaacatattg tagcgcatct
    67021 gatgaataat gaacggtgct tcctttcttt ttaaaatttc agtcattttt tttgtatctt
    67081 ctccattata gttggatata ccaatatata atgcttttcc ttgtcttaca agttgcatca
    67141 aagcttctgc tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga tagaagatgt
    67201 caacataatc tagccccatt ctttggagac tttggtcaca acttgaaatg atggatttac
    67261 gagagcccca ttccccatat ggcccttccc acatatgata acctgctttg ctagaaataa
    67321 tcagttcatc acggtaggct tgaaaatctt ctctaaaaat acgcccaaag tttttctctg
    67381 cagctccagc agggcctcca taattattcg caaggtcgaa atgggtaatt cccatgtcga
    67441 atgcgcctct taaaatctct ctttgattat ggataggatc ataatcacca aaattttgcc
    67501 ataatccgag agataaaatc ggtaatttca aacctgaatg accggattta cgatattcca
    67561 ttttttcata acgtgcacta tctgcaatat acaatttttc tcctcctcat caaatctttt
    67621 taaaacgctt tcttttttct atttttagtt tactacattt taaagaaaag gagttaatca
    67681 aaatgatttt ttcgtatatt tttttgccat ttgtttttta ctcgttgata gatcagaaat
    67741 tgattacgtg cttgctggat ttttttataa cgtttcggtt gttttttgta aaattgctga
    67801 tgatagtttt ctgctggcca aaacgtaccg gctggttcga tttttgtgac gattggttgg
    67861 tggaaagttc ctgaactgtc tagtttctgt tttgattgtt ccgctaattc tttttgtcgt
    67921 tcgccttgat aaaaaatcac tggacgatat tgcttccctc tatcttgaaa ttgtccaaat
    67981 gtatctgtgg gatcagtaat ttgccaatag attgtcagca actcgctata acttatttct
    68041 cttgtgtcat aaatgatctc cacagcttcc acatgacctg tgtaaccacc acttacttga
    68101 tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacaga taggatccct
    68161 tttttcgatt caaacggctc gaccatacac cagaaacatc cccctgcaaa tactgcacgt
    68221 tcttgatagt cagcatcttg catggcatgc ttggaaaagt catatttttg ctccttatca
    68281 aatccttccg taatcaataa ataaaagtcc attacatcag gcgtcagatt cattcggata
    68341 gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttaacgga tatattttct
    68401 cttatgtcat tctttgtctg aatcaatact tttcgttccc aatctcgtgt attcggattc
    68461 aaaatcagat tataaagtgt ttgaatgaat atttgttcct catccataga ttctcctcct
    68521 ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc
    68581 gattacttca tccccctaca gatttttcgt ataaaaaaga gagaatcctt atcgacagat
    68641 tctccttttt aatcaattta ttttataaat gaaaagttaa tttacctttt atggaaaact
    68701 attttaaaaa ataaacataa atatgcagtt aatgtaacaa gataagaaat aatagtcgtt
    68761 actgtataac ttgtgcttac tttaatatca gcaactcctt tgactccgct tatttgtacc
    68821 aatccattag cattcacctg ctcctttaca ggttgatgat ttatttctac attcgttcct
    68881 aaataagcat atacaggaag tgtgattact tggctcttct cattagttac tctatatatg
    68941 atagaactac cagttgtttt ataaggaagg tttacccatt gactgccaat ctttgcttcg
    69001 tgttttatta tatgctcctt attctccaat gaagcgatag gagaataatc atataaaaaa
    69061 taatccattc ctttagtggt atacgcctct actgtttctt tgtttacaac aatatgtccc
    69121 ggctgagaaa ttattttttg attgaaattt tttttactag aaacagttaa agtgattaat
    69181 aacaaaacag tcataaataa ataaaatagt tctcttgtct tattgtatct tctatttttg
    69241 aaaaatatca tcgttaatat cagagaagag aatattattt gcaagcttaa gatccgccaa
    69301 ggaaattgta acaatttggc tggcgtattt tgcagaagat accatggcac caaactagtt
    69361 gtagctagcc aagtcaaaca cgtaccataa taaatctttt tttctatgtt tgttaatttt
    69421 ttaaataaaa tcaggggaat acataaagat aacagaccaa ctagtcccat tgagtaagta
    69481 gttaagtcgt tgttcatagc ttcagtcaga ctatcgaaca cattagatgc atgagcatat
    69541 aaatttgtca cgctaggttt gttgattttt tggtagagca tttgttcaaa catcggatac
    69601 caaaaagcac tcgtcagaac caatgtcatt cctgccgcac taaataattt tttaaatata
    69661 gcaataaaat ctgcattctt gaaaccttta gaaaccaaac gaataaaaat aaaaatgata
    69721 attaatgctg tactcattaa tgttgatagt acatggctgt acactagcaa actcatactc
    69781 aatgctagct ttacagacgg atacttttct tcttttccag agatgatttg ataagcataa
    69841 aataatacag ggataacaag tgttattgcg atagcttcgg caattgcgga acgatagtag
    69901 atgtctactg tccgataagc agagaaaata tagagacaac taaataaaaa tgccgctttt
    69961 ttttgtttta aaaaccgctc gccataatga taagctacga gtgttgtaca caagttcaat
    70021 aaccaaacgt aaataatata gcttacaatc aaattttttg tcatccaata taacattaca
    70081 gctggaaaaa aagtcagaaa gggataaaaa taatttacac catatccagt ataattgaat
    70141 gtctcataat taatcggact ggtaaaaata gtcgataacc ccttgattcg agataagtga
    70201 aaagaaagat ctccagtggt ataaatactc tgtgtatttt tgaaaaagat gaaagaatat
    70261 atactactaa ctataattaa tccactcaat attactactt ttttatttat tttcattctt
    70321 cttgtttatc tcctttttag tcacatatcg agaatctttc aacttattag gtgcaaatcg
    70381 tttaaaactt gaatacacca ctaaacaata agtcagaata ctgatccata aagccatgag
    70441 agcagttgga gtatattttg ttgtaatttc tatcttattt tctccagcat ttacatccac
    70501 attcactaag ccatgttttt tataagagtc atttacgact ttaccgttta cttttacctg
    70561 tgtacctaaa taagcatata ctggtactgt cactttttgc tttttgttgc ttgccactgt
    70621 gtaagtaatg ttattatctg cagctttata cttccctttt tcccacgatt ctcctacttt
    70681 tacttcatgt ttttctaagt gaggcctttc ttttatcgct tctgttgggg cgtaatcgta
    70741 taatcctccc atatctgatg ttgtataacg tgggagtgtt tcttcattga taacaatatg
    70801 accgtgtttt gattggataa cttttgcgta attttctttt gctgctatag acgtgacgag
    70861 tagaaacacc acagtagcgc caatgattaa ccatgaatat tttttattca tagtcttttt
    70921 cataaaaaca agcgttatga tcattgaaga aaaaaagatt tggataccta agatacgcca
    70981 aggaaactgg agcaagctta tcggtgtttt ttgaaacaaa aaccatggaa ataaattagt
    71041 tacgcatacc catgtagaaa ctacgccaac atagatgatt ttttcttttt tctccatttc
    71101 tttcagtatg aataaaggaa aaatcaacgc aaccaatcct aataacccca tagtgtatgt
    71161 tgtaatatcg ttatttaacg cttgaatgat actatctgaa atagaaagtg cttctagctg
    71221 caaatctgtt ttgaagggac tgtgtatttc ttgataattc atctgctgca acataggata
    71281 ccaaaaatag ctagttaatg cacaagtcaa aaaaacagcc tttactaaat tcacgaccac
    71341 aaattttata tcgtttttta tttgcttgct taaaaacaaa cgaactataa tcatacaagc
    71401 aatcaatgca ctacacaata gtgtagttaa gacatgagta tacacaagta gacttatact
    71461 gacagataaa gcgatgactt ttttttctct attgaatatc aatccataag cataatataa
    71521 aacagctggg ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat aatagatatc
    71581 aactgttcga taagttgcaa aagtatataa acaactaaat aaaaaagcag catctatctt
    71641 tttaaaaaac ttttctccat aataaaaac
//
LOCUS       DACCDF010000004        71017 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCDF010000004 DACCDF010000000
VERSION     DACCDF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.004"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            369..1472
                     /locus_tag="IVK51_000204"
     CDS             369..1472
                     /locus_tag="IVK51_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6429829.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1756..2436
                     /locus_tag="IVK51_000205"
     CDS             1756..2436
                     /locus_tag="IVK51_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6429830.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2526..3560
                     /locus_tag="IVK51_000206"
     CDS             2526..3560
                     /locus_tag="IVK51_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP6429831.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3574..3909
                     /locus_tag="IVK51_000207"
     CDS             3574..3909
                     /locus_tag="IVK51_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429832.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3893..6373
                     /locus_tag="IVK51_000208"
     CDS             3893..6373
                     /locus_tag="IVK51_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429833.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6429..7988
                     /locus_tag="IVK51_000209"
     CDS             6429..7988
                     /locus_tag="IVK51_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6429834.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7985..9349
                     /locus_tag="IVK51_000210"
     CDS             7985..9349
                     /locus_tag="IVK51_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6429835.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9520..10590
                     /locus_tag="IVK51_000211"
     CDS             9520..10590
                     /locus_tag="IVK51_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429836.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10605..11273
                     /locus_tag="IVK51_000212"
     CDS             10605..11273
                     /locus_tag="IVK51_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429837.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11293..11871
                     /locus_tag="IVK51_000213"
     CDS             11293..11871
                     /locus_tag="IVK51_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6429838.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12274..13185
                     /locus_tag="IVK51_000214"
     CDS             12274..13185
                     /locus_tag="IVK51_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6429839.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13441..14358)
                     /locus_tag="IVK51_000215"
     CDS             complement(13441..14358)
                     /locus_tag="IVK51_000215"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP6429840.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14560..14997
                     /gene="zapA"
                     /locus_tag="IVK51_000216"
     CDS             14560..14997
                     /gene="zapA"
                     /locus_tag="IVK51_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP6429841.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15055..15603
                     /locus_tag="IVK51_000217"
     CDS             15055..15603
                     /locus_tag="IVK51_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP6429842.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15718..18078
                     /locus_tag="IVK51_000218"
     CDS             15718..18078
                     /locus_tag="IVK51_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP6429843.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18165..18479
                     /gene="trxA"
                     /locus_tag="IVK51_000219"
     CDS             18165..18479
                     /gene="trxA"
                     /locus_tag="IVK51_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP6429844.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18661..20439
                     /gene="uvrC"
                     /locus_tag="IVK51_000220"
     CDS             18661..20439
                     /gene="uvrC"
                     /locus_tag="IVK51_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP6429845.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20584..21483
                     /locus_tag="IVK51_000221"
     CDS             20584..21483
                     /locus_tag="IVK51_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429846.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21476..22738
                     /locus_tag="IVK51_000222"
     CDS             21476..22738
                     /locus_tag="IVK51_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429847.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTILLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22901..24799
                     /locus_tag="IVK51_000223"
     CDS             22901..24799
                     /locus_tag="IVK51_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP6429848.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25098..25817)
                     /locus_tag="IVK51_000224"
     CDS             complement(25098..25817)
                     /locus_tag="IVK51_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP6429849.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26154..26486
                     /locus_tag="IVK51_000225"
     CDS             26154..26486
                     /locus_tag="IVK51_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429850.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26473..28476
                     /locus_tag="IVK51_000226"
     CDS             26473..28476
                     /locus_tag="IVK51_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP6429851.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKHQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28495..28965
                     /locus_tag="IVK51_000227"
     CDS             28495..28965
                     /locus_tag="IVK51_000227"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP6429852.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28986..29570
                     /locus_tag="IVK51_000228"
     CDS             28986..29570
                     /locus_tag="IVK51_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP6429853.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29585..30571
                     /locus_tag="IVK51_000229"
     CDS             29585..30571
                     /locus_tag="IVK51_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP6429854.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30561..30872
                     /locus_tag="IVK51_000230"
     CDS             30561..30872
                     /locus_tag="IVK51_000230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP6429855.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30894..32675
                     /locus_tag="IVK51_000231"
     CDS             30894..32675
                     /locus_tag="IVK51_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP6429856.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32668..34044
                     /locus_tag="IVK51_000232"
     CDS             32668..34044
                     /locus_tag="IVK51_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP6429857.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34051..34686
                     /locus_tag="IVK51_000233"
     CDS             34051..34686
                     /locus_tag="IVK51_000233"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP6429858.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34716..36071
                     /locus_tag="IVK51_000234"
     CDS             34716..36071
                     /locus_tag="IVK51_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP6429859.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36263..37657)
                     /locus_tag="IVK51_000235"
     CDS             complement(36263..37657)
                     /locus_tag="IVK51_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6429860.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFNSPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37749..38720
                     /locus_tag="IVK51_000236"
     CDS             37749..38720
                     /locus_tag="IVK51_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP6429861.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38717..39499
                     /locus_tag="IVK51_000237"
     CDS             38717..39499
                     /locus_tag="IVK51_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP6429862.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39668..40039)
                     /locus_tag="IVK51_000238"
     CDS             complement(39668..40039)
                     /locus_tag="IVK51_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP6429863.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40173..40616
                     /locus_tag="IVK51_000239"
     CDS             40173..40616
                     /locus_tag="IVK51_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6429864.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40659..41468)
                     /gene="yidA"
                     /locus_tag="IVK51_000240"
     CDS             complement(40659..41468)
                     /gene="yidA"
                     /locus_tag="IVK51_000240"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP6429865.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41487..42854)
                     /locus_tag="IVK51_000241"
     CDS             complement(41487..42854)
                     /locus_tag="IVK51_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6429866.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42838..43668)
                     /locus_tag="IVK51_000242"
     CDS             complement(42838..43668)
                     /locus_tag="IVK51_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP6429867.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43831..44268
                     /locus_tag="IVK51_000243"
     CDS             43831..44268
                     /locus_tag="IVK51_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP6429868.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44344..44964
                     /locus_tag="IVK51_000244"
     CDS             44344..44964
                     /locus_tag="IVK51_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP6429869.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45153..45659
                     /locus_tag="IVK51_000245"
     CDS             45153..45659
                     /locus_tag="IVK51_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP6429870.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45676..46575
                     /locus_tag="IVK51_000246"
     CDS             45676..46575
                     /locus_tag="IVK51_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP6429871.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46662..47546
                     /locus_tag="IVK51_000247"
     CDS             46662..47546
                     /locus_tag="IVK51_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6429872.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47738..47809)
                     /locus_tag="IVK51_000248"
     tRNA            complement(47738..47809)
                     /locus_tag="IVK51_000248"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47774..47776),aa:Arg,seq:ccg)
     gene            47869..49203
                     /gene="rpoN"
                     /locus_tag="IVK51_000249"
     CDS             47869..49203
                     /gene="rpoN"
                     /locus_tag="IVK51_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP6429873.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49406..50443
                     /locus_tag="IVK51_000250"
     CDS             49406..50443
                     /locus_tag="IVK51_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP6429874.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50484..51485
                     /gene="gap"
                     /locus_tag="IVK51_000251"
     CDS             50484..51485
                     /gene="gap"
                     /locus_tag="IVK51_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6429875.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51595..52785
                     /locus_tag="IVK51_000252"
     CDS             51595..52785
                     /locus_tag="IVK51_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP6429876.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52889..53644
                     /locus_tag="IVK51_000253"
     CDS             52889..53644
                     /locus_tag="IVK51_000253"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP6429877.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53760..55058
                     /gene="eno"
                     /locus_tag="IVK51_000254"
     CDS             53760..55058
                     /gene="eno"
                     /locus_tag="IVK51_000254"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP6429878.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55458..56597
                     /locus_tag="IVK51_000255"
     CDS             55458..56597
                     /locus_tag="IVK51_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP6429879.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(56664..57239)
                     /locus_tag="IVK51_000256"
     CDS             complement(56664..57239)
                     /locus_tag="IVK51_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6429880.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(57244..58218)
                     /locus_tag="IVK51_000257"
     CDS             complement(57244..58218)
                     /locus_tag="IVK51_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP6429881.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(58242..58286)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            58501..59468
                     /locus_tag="IVK51_000258"
                     /pseudo
     CDS             58501..59468
                     /locus_tag="IVK51_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            59581..59859
                     /locus_tag="IVK51_000259"
     CDS             59581..59859
                     /locus_tag="IVK51_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6429882.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            59977..60213
                     /gene="secG"
                     /locus_tag="IVK51_000260"
     CDS             59977..60213
                     /gene="secG"
                     /locus_tag="IVK51_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP6429883.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            60325..61071
                     /locus_tag="IVK51_000261"
     CDS             60325..61071
                     /locus_tag="IVK51_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6429884.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            61074..63431
                     /gene="rnr"
                     /locus_tag="IVK51_000262"
     CDS             61074..63431
                     /gene="rnr"
                     /locus_tag="IVK51_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP6429885.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWATELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            63453..63917
                     /gene="smpB"
                     /locus_tag="IVK51_000263"
     CDS             63453..63917
                     /gene="smpB"
                     /locus_tag="IVK51_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP6429886.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            64219..64938
                     /gene="atpB"
                     /locus_tag="IVK51_000264"
     CDS             64219..64938
                     /gene="atpB"
                     /locus_tag="IVK51_000264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP6429887.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            64991..65206
                     /gene="atpE"
                     /locus_tag="IVK51_000265"
     CDS             64991..65206
                     /gene="atpE"
                     /locus_tag="IVK51_000265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP6429888.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            65297..65821
                     /gene="atpF"
                     /locus_tag="IVK51_000266"
     CDS             65297..65821
                     /gene="atpF"
                     /locus_tag="IVK51_000266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP6429889.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            65808..66350
                     /locus_tag="IVK51_000267"
     CDS             65808..66350
                     /locus_tag="IVK51_000267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP6429890.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            66373..67929
                     /locus_tag="IVK51_000268"
     CDS             66373..67929
                     /locus_tag="IVK51_000268"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP6429891.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            67943..68845
                     /locus_tag="IVK51_000269"
     CDS             67943..68845
                     /locus_tag="IVK51_000269"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP6429892.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            68868..70274
                     /gene="atpD"
                     /locus_tag="IVK51_000270"
     CDS             68868..70274
                     /gene="atpD"
                     /locus_tag="IVK51_000270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP6429893.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            70288..70710
                     /locus_tag="IVK51_000271"
     CDS             70288..70710
                     /locus_tag="IVK51_000271"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP6429894.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT        23564 a        12441 c        15097 g        19915 t
ORIGIN      
        1 aataccgagg gtcagctgat tgaacaccgt tttatcgaga ggttgtgaaa aagtcgctta
       61 gtttcgagta gtaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttga
      121 cttaataccg agaaaatggc ttttgaacac cgtttattca aaaaaaggga ctttgacact
      181 acttttgtca aagtcccatc ggttttttat ctatttttat tacaatcatt tcttccagag
      241 tagtgacatc tggaatggct gaaatgtaaa cagattattt gcctgtatca atgtcgaaga
      301 gtaatataaa aagtgtaaac gctatcatat tgttcatcat tcaaatttta tagagagaag
      361 gcattgagat ggaaaaaaca tatcaagcag gtacaaatga aggaatagta gattttatca
      421 atatggaaga tttagaaatc gcggcttcac aagtgattcc agctggtggt tatggttaca
      481 tcagcagtgg tgctggcgac ttatttactt atcaagaaaa tgaacgggca ttcaatcatc
      541 gtttgattat tcctcatgta ttaagagatg tagaattacc cgatacaact acacattttg
      601 acgaagaaat gctgactgca ccaatcatca tggcaccagt tgctgctcat ggattagctc
      661 atgtgaaggc ggagaaagct tctgcaaaag gtgtagcaga ttttgggacg atctatacag
      721 caagttctta cgcttcttgt actttagaag agataagaga agcaggcggg gagaaagcac
      781 cgcaatggtt tcaattttat atgagcaaag ataatggaat caatttggat attcttgaag
      841 tggccaagcg taatggagca aaagcaatcg tattgacagc ggatgcaaca gttggcggaa
      901 accgtgaaac agaccgcaga aacgggttta ccttcccgct gccaatgcca atcgttcagg
      961 cttatcaatc aggagtaggc caaacgatgg atgctgtata taaatcttcc aaacaaaaac
     1021 ttagtccaaa agatgtagaa tttatcgctg ctcattctga tttgccagtc tacgtgaaag
     1081 gtgttcaatc agaagaagat gtctatcgat cattagaatc tggtgctggt ggaatttggg
     1141 tatctaacca tggtggccgt caattagatg gggggcctgc tgcgtttgat tcgttgcaat
     1201 atgtggcaga agcagtagat aaacgtgtac caatcgtgtt tgacagcggt gtacgacgtg
     1261 gacaacatgt tttcaaagca atcgcatcag gtgccgatct tgttgctatt ggcagacccg
     1321 ttatttatgg attgtcttta ggaggaagta caggagtcca tcaagtgttt gatttcttta
     1381 aaacagaatt ggaaatggtc atgcaattag ctggaacaca aacagtagaa gatatcaaaa
     1441 aaataaaatt gcgtgagaat cgttttattt aagaaataaa tgacgaaaaa aataggagtg
     1501 cggcagatga tgccgtactc ctatttttat agttcaagga tgtcactaat agaataattt
     1561 gaaacgaaaa aataaaattg aaatattttt tcatacttaa taaaaaagga agaatattca
     1621 tgtaaacgat tttattgttt ttttctcttt tatacaaaaa gggtcataat ttcaagtaag
     1681 cggatgaatt catgaggatt ttttgcatac tgtctctact atacttaggt cactaagaaa
     1741 aaggagtgat ttattatgaa aaagaacatg attgggacat tattactttc aacacttctt
     1801 ctaggcggct tggcaacacc agcttttgct gagggtcaag cgacttcaaa aggagatatt
     1861 acctttactg aaccaacaaa taccgttgag cctcttaacc ctactgatcc ttcaaaacca
     1921 gtagagccag cagatccgga aaatccagca actggccaga caggttcttt aacattggat
     1981 gtagttcctg aattgccatt tggtacacac gagattgaaa gcggaacaaa aacgtatcaa
     2041 gtagacgctt caaaaaacga tacaccttat ctccaagtat cagatcgtcg aggtgtaggt
     2101 gcagatggcc aagcacaagg gtggaacgtg acagtttcgg tttcagattt tgtaaacggt
     2161 tcacaagttt tgcaaggcgc tgaattagat tttggaacat ccactgttaa aagtacgtca
     2221 gataacgaat ccactggacc aactagtcaa actgtaactg ggttgagtaa agcaagtgca
     2281 gcgacaccaa tatttacagc agcaaaagat caaggtctgg gaacatggct ttctgtttat
     2341 gatccagcaa atatcacact gaaagtgccg aaagcagcag ctggaacatt cacggctgat
     2401 ttgacttgga acctcgtggc tggcccagta gcatagagta tgaaataaat tcaacaaact
     2461 gcggtgaaaa tactataggt gaaggatttt ttaccgcagt tgttgttgaa aggatgtggt
     2521 ggacaatgaa tcaaagtaaa cgactgtttg tttcgttttt ttctatttta agtctatttt
     2581 tcatttttcc gtcaatcagc caagcaaagg attcagcagg agactttgga atcaaaccgg
     2641 tatttccaga gaaccaaata gataaagcaa tcggttattt tgatttatta gtggcaccgg
     2701 aacaaaatca gacactagaa gtaattatca gcaattcttc agatgaagaa cgaacatttg
     2761 aggtctctgt caatccggcg gtaacgagtg atggtggaac gatcgattat agtcaaaaaa
     2821 acccaacgtt agatgagaca ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa
     2881 aggaaatcaa tgtatcagca catgcagaaa caactatacc tatagaagtg aaaatcccag
     2941 caaaaagctt taagggacgt gtattagcgg gtattcatgt ttcacctaaa gaagaagctg
     3001 aaacagaaaa tgctacagaa ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag
     3061 tcgttttaca agaaagtcaa gagacgatag aaccggatct gaaactttta tcaggagatt
     3121 tggacgaggt caacgcaaag ccaactgttc agcttcgttt ccaaaatcct cagcctagga
     3181 ttatcagcaa tttgatattt acgagcaaga tcttttatga aaatcagctt tatattgaaa
     3241 atacgtctaa tgaattttta gttgcaccaa attcgaattt tcacttgaat cttgatttag
     3301 ctggtgataa agcaaaagcc ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca
     3361 gtaatgaatg gcgatttaca caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa
     3421 atgaaaattc cgtttttgca gtacaagaac aatctttccc atggctttat gcaggattgg
     3481 ttgctgcagt tctagtgtta tggttgattt tgctgttttt gtgggtccac aagaaaaaga
     3541 aaaaaggaga aacaacatga tgaggtgatt tcgttgaaaa catcgatctt attgggaggg
     3601 attattgcaa gtttgctgtt cttcagctgg caagagccag tccaagcaga taccaaaagt
     3661 tcaggtacga ttacatttta tgatctaagt ccagttccgc atccaagtgg ttcagtcaat
     3721 ggcgcatcaa aacagcagac acagctaccg atttataaag aatctaatca acatggcgta
     3781 ttcccggaga cgaatgaaga aaagatccgt tttctttatt tgatcattgg tatagaatta
     3841 tggcttcttg cgtggttgtt gttatggaga agaaaggaaa aggaggatga acatgttgct
     3901 acaaaataaa ttttcatttt ttcgctcctt cttttttact tcgctttttg ttttaagtat
     3961 atttatcttt tcaccttcgg cagaagctgt tggagcaacg cctcctgcca tttcttatga
     4021 tccatacacg tatgaaaata tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg
     4081 gaagcttgtc caactaactg gggctaacca attgcctaaa ttgactgcta cttatttgaa
     4141 cgatggttca aatgatcctt ggaaaggtgg cacggtccgt tttcattggg aaagccatgt
     4201 gaaactaaca aatgtacttg atttggtgaa tatcactgca caaggatttg catttgtggc
     4261 gatcatcaat ttgccggaac atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa
     4321 caacgcctac ttgtatattg ccggtgcccc catcaaggtt actagcggta gtttattatc
     4381 agcaggaagc cataccttgc gttttgggat ggggtcgtgg gatacaaata ctctattggc
     4441 agatattatt gctattatta cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt
     4501 tgatgttaca gctaatgtcg cgaagatgac agaaaacgga gataaatatg atacgagtcc
     4561 aaacaaaatt ttgaccaagg gtaaattaca accttctatg tctaaaaagg ccgttttttc
     4621 tgttgatttt tatgattcga ataatattat ccaagatcgt atgttcaatt cgattttagc
     4681 tccagctgca ggtagatttt atcctaaatt aactggcgga aacttgaatc caggagcata
     4741 tgcattttct gcaagtgcag agataaatac gtggaatagt tacatctcaa caagtgattc
     4801 aacaggaaaa tataatacat ttgctgatac cacagaatac gtggatggaa ataaccagaa
     4861 ccttccaggc tctaccaata gtcggacatt gacgatgggg ctgactgaag gacgttttga
     4921 tcagttgcca tttagtcgct ttgatcgcgt agtcaactat tttacaggaa aaaacgttac
     4981 acaaggagca atactgactc atactccagc aagcacaagt ggattgaatc agacagccaa
     5041 tatcgtgtac agtgggaaag atgcgcaagg cggattgctt tctcctgttg ctttaaaggt
     5101 attggagcag tacaagctag acggaacggt tttaccaaca aacttaacag ccgatagtca
     5161 gtggggaaag ctcctttctt ctgcatcgta ccaagtcacg gccaattggt cagcaactga
     5221 actaaaaaag ggttcgatcc attacaggct ttattcgaaa ggaacccaaa aattagcgac
     5281 aggatggaca gatcaacttt tccaaacgat ccaatcaaat aacgggggtg taaatacagc
     5341 tacttctaca ctacctgcat tggcttcagg gcaatattat tttgattatc gcttggtgga
     5401 cgatgtactt actaacatgt atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg
     5461 aatcaccact cttcctcaaa taacagtggc taattttcca tctgtgagtg cagatagtca
     5521 gctaaaaaac cttagcagac aacctgagac aggagatccc ctgtctgctc tatcaggaga
     5581 tacgattcaa gaaaatgtga cattcacgtt aacaaaatct ggggatgcga taaccgacaa
     5641 aaaaatcacg atctcacttc ctgaaaatac tacttatatc aatggttcct taaaattaaa
     5701 tggtacaact atcgcagata ccggcattca acaaggagtt tctgtcccag cagatctatt
     5761 gagcaaaata ggagatacca ttcatcttac ttacaactat caattaaata ctgtagatac
     5821 ttctgtacaa tccgtcagca tactgacaaa ggcagctgta ctttccagca atattacatt
     5881 agcagatggt gctaagcttc ctaacccagt tgtccaaaca tctgctaaaa caattcttgt
     5941 ccccaaacaa gaattaacat tagttaatgt accagatgat tttacatttg gtaacgatct
     6001 ccccaaaccg ttgaagacaa gttattatga agcaaaaggc gatttttctt ttgatgtgcg
     6061 ggatactcgg cttccaagca cgtctccttg gcaactgaca gggacattga cttctttatt
     6121 taagaatgat caaggtcaag aattaagcgg aacaaaatta tacttcaatc attctggatc
     6181 aaaacaattg attcagcaag gtcaaaacac gttgatttat gagagtgatg gaacagctaa
     6241 aggagaggtt ctcgttgact ttcccgatac cgatggttta ctcttagagg taaatagcag
     6301 tacgaatgct caacctggcg caacgtatca aggcatggtc acatgggaac tgacagctgg
     6361 gccaaccagc taagtgatta gaatttgtat ggatctgtga taactgtcac agatcctctt
     6421 tttcaaggat gagaaggaaa cttctgctgt taaaatcaga aatgcttgga ggagtggact
     6481 tggaactttt cagtttgctt gaaaaaaatg aacaacggca aatcaaactt ttccaacttt
     6541 tattagagga aaaaggagag atttccgtta ataacgtact tacccgctta tcagttgatc
     6601 gggctacctt gaaagaagat ctgttttatt tacaagctaa cttggaaaat atcaaacaat
     6661 tggaaattgt tatccaagaa gggaatattt tattaatcag acatggaaat atagcagcag
     6721 tcgatgttta ttatcattgc tacttgcgct attctacaaa atatcagata cttctttatc
     6781 tgttggaaaa tggctcattt gatcggcaga aacttgtcat ggaacttaat atcagttcgg
     6841 ctactttgtc tcgcagagtg aaggaattaa atgattcttt aaatgagttt catctgaaaa
     6901 taaaaagcgg gcggctgacc ggtccagaaa gtcaaatttg ttatttttat ttccaattat
     6961 tttggtttgg acgtccttat cttgaaaaca aaaaagaata cagtgattta tcaggaaaca
     7021 cttttttaga tctgctgcaa aaagagttta gttttactat tacagaagaa ggacgattga
     7081 aatttgaaat atggctttcc attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac
     7141 cagattatag caatcttcct gttggattgt ttcaaacgac acaattttta ttggacctcc
     7201 atacattttt gacccgttac ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt
     7261 tggtttttta tcttttcatg atcagtaact ttacattaga tacacataac tcgcatgtcc
     7321 aggagttttt gataagcgaa aaatacaaag acgacgtagt agctcaattg aatcgaacgt
     7381 ttaaacaatc gatagaagaa tatttctctt tttatgtttt tccagaagag atttcgcaaa
     7441 agattgcgtt gacagtgatg caaaatcacg ctcattttgc ctattttaga ggatggatga
     7501 atatttttgg ccaagaagga ttaaaacagc ggttaactga agtagaagat aaacaattcc
     7561 tggttttatg tgaaagattg gtcagacaaa catttgctat tgttgatttt actgaagtag
     7621 acgaggaaaa tgcccgcttt gaattatttg ctcgctatgc atctattttg cggacgctga
     7681 tcaagcagtt agaattacgt ctgcaaattg cttatgatct tccttatgaa aggctaatgg
     7741 cggatatcgt gaaagaaatc atccaggaac aattgctgaa tacgatcaaa tatgatttag
     7801 tcgaatacga gaaagaaaag cagtatgata tcattttgac tagccaattg aaagagtacc
     7861 cgtcacagaa gtccgctaaa gtttttgtat tcactagcaa cgaatataat tatgatttcc
     7921 cctatttgaa ccaattttta aaagaatgtt atttagaaaa attgaattta aggatgaata
     7981 aaacatgaaa atcgtgatca ttggtgcttc ccatgcaggc atcacagctg ccctaaatct
     8041 aagaaaactt caaccgaaag cagaagtttt actgatcgat gaagatcata aagatggact
     8101 aggctatgtt tcaaatggaa tcaaccttta tttaaaagga aagatacgta gtttagctga
     8161 agttgcccat aacatgcgga ctcttcaaga aagtggggca aaactgatca ccgaatggag
     8221 agtaactgaa cttgatccag acaaacatca gctcatcctc gcatccaaag aaggaaaaaa
     8281 cgagaccata acctatgata aattaatcgt agcaacaggt tcctctcctg tcaccttgta
     8341 taaacaaatt gaagctgaaa atgtctacac ctataaaaat ctagtgcaat ctaagcaagt
     8401 acttgccgct ttaaaagaag caaaagaagt agtgatcttc ggtgctggtt atattggatt
     8461 ggaattagca gatgctttga gaaataaagg atacattatt catctagttg attatatgcc
     8521 gaatgtttta tcaagatatt tcgataagga tatgatcaac agttttcaaa atcagctgca
     8581 aactaagcaa atcaattttt atccaaatga attcttgatc gattggaaaa aatcagaaga
     8641 gaaagtagta tccgtacaac ttctatctca agcaataaag gcagatatgg tcattttttc
     8701 tgcccaaact cgtcctaata cgacattgtt aaaagaaaaa gtggctttgt atgaggatga
     8761 gactgtgata gtcaatgaat atctgcagac aagtgatccg gacatctatg cgattggaga
     8821 tatcgtccca gtcagttttg acaaaaataa acggcatctc tttttacctt tagttactcg
     8881 agctgtacat atggcaagag cagtagcgct aactttgagt ggtcagccaa ccgcttacga
     8941 cctcagacaa aagataacag cagcagtcat tatagactac tttttaggaa ctgtagggct
     9001 gacagaagat gaagcgccat tcttggaaca aaataccggt tcgtgttcag gagaatttga
     9061 tctatttcca caatacgatg aagaaaacaa aacagtaaat gcaaagctga tctaccatcc
     9121 ggatacatta gaaataattg gcggacagct gataagccaa gaattcttat tatcagattt
     9181 gaacctgctt gcggatatcg tgaaacacaa aactacgatt ccacagctag cagttgagga
     9241 ttttggattt ctgtctgaac acacctctcg ttttcactac ctcaatgagt tagcttttaa
     9301 agtactagct aagagagcaa atagcaatag agtagataaa agactgtgag aaatatcacg
     9361 gtcttttatt gtgtggggat ataataaata ttaatcaatc gagaataaaa aattcatata
     9421 atgaaatagc ggaagaattt tttgagcata atattcgcca aaaatttatc aagtgataaa
     9481 attgttgtat aaaatgatta tttcaggagg aggagttaaa tgtttttagc attaaatgaa
     9541 attatgcatt caaagttacg ttatgcgtta gttgcagggg tgatgttttt gatcgcctac
     9601 ctggtatttt ttttgactgg gttagcttat ggcttagctc aagataatcg aacagcagtc
     9661 gacaagtggg aggcagattc gattgtgctt tcgaaagatg caaattcaaa tctagggatg
     9721 tccatgatta ctaagaagat agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc
     9781 cagacacctg gagtcgtgac tagtaaagac agtacagaag aaggaaaaat caatgttagc
     9841 ttcttcggta tcgataaaaa tcaattcatc atgcccaatc ttgttgaagg aaaagctttt
     9901 gataacgatg atgaggcagt aggcgatatt agtttgaaag aagaatatgg tctagccgta
     9961 ggagatacag ttaagttatc tggtagtgac aagacgttta aactaactgg ctttacggat
    10021 catgccaaat tcaatgtctc accagttctc tatacaacca tcaatgctta ccaagagatc
    10081 cgcttcgaaa aagaagatac aagtgaaaat gcacggatta acgcaatagt tgtcagaggt
    10141 aaaatcagta atttacctga agatttagaa cagatcaaaa tttctaaatt catcaatgaa
    10201 ttacctggat ataatgcgca agtattgact tttggattta tgatcggatt tttgatcgtt
    10261 attgcggcta ttgtgatcgg gattttcatt tatgtactga cgatgcaaaa gattaatatc
    10321 tttggtgtaa tgaaagcaca agggatcaca ggaggattta ttgcgcgttc tgtagtggca
    10381 caaacattca ttttatcatt tgttggtatt cttcttggtc tattaggaac agtgggaaca
    10441 tcccttgttc tacctgatgc ggttccattc caaagcaatt ggctgttttt tggcgtcatt
    10501 agcttgttga tgctggtagt agctgtgtta ggcgcactat tttctgttcg gacaatcgta
    10561 aaaatcgatc cgttgaaggc aattggataa gaaggaggaa gagcatgaaa gcaatagaat
    10621 ttaaagaagt agaaaaaagt ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa
    10681 caaccatgag tatcgataaa ggggaatttg ttgcaatcat cggtccttct ggttcaggta
    10741 aaagtacttt tttgactttg gcaggcggat tgcagacgcc tagcagtggc tctgtattta
    10801 tcaatgatca agaattcagt aatcaaaaag aaagtgaacg cgttaaacta cgttttaaag
    10861 aaattggctt tattttgcaa gcttccaatc tggttccttt tctgacagta aaaaagcagt
    10921 tattattagt agataagatc aaacatgaac atcgtcaaaa agaagcaaaa gaattatttg
    10981 agcagcttgg cgtggataag ttgatcaaca agtatccaga agaactatct ggtggagaac
    11041 ggcaaagggt cgcaatcgct cgtgcattgt acaatgatcc gtccattatt ctagcagatg
    11101 aaccaactgc tagtttggat tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag
    11161 aatcaaaaga caaaaataaa gcaacgatca tggttactca cgatacacgg ctgattgaca
    11221 aatgcgatag aatatttatg atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa
    11281 gcaggtggaa aaatggtcca tcgatacaac aaagatacac agcaaaaaat tttgaaagaa
    11341 gcgactgact tgtttatgtc caagggatat ctcggcactt caacaagaga gatcgcacaa
    11401 aaagttggga tcacccaacc caatctctat cattatttcg gtgataaaga aaaactatat
    11461 acggcagttt tagaaaacca cttaaaagat gttggaaaag ctttgagaga aatcgttcaa
    11521 acaagcgaaa cgggatttca agtaaccttg acaaaaatgg cgcagttttt gattgagaca
    11581 catctggtcg atttgtttct gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca
    11641 agagatcatc ttttctttct atggaagaaa aattatcgtg aaccttttga agaaatcttt
    11701 tcagcaaacc aatctgtgct aagaaatggg atttcaaaag aaatcgcagc aaggcatttt
    11761 ttcttagtgt tagcacctta catcactcgg tcgaaaagac ctgtggaaga accgctaaat
    11821 gtggcgcaat tgatcgattt atatttgaat ggtgtattag gagaagcgta aagctgttat
    11881 atagggagaa aaatagttag gtgaagaagc aagatatgac gaagatcgtt atatcttgtt
    11941 ttttttatat aggtagtttt tcataaatac aaatttgtta ttctctcatg attatcaggg
    12001 tatattttta tttattatgt cggtgttcca ggaagtgttt taatttaata acgaagaaat
    12061 taattgtttg tagaatgaag gaaactattt attgggtaga tgaatcacca cgaatagtca
    12121 aaaggagatt tcaggatatg ccaaaaatga aaaaaatcat tttagcagta atgttatttt
    12181 ctacggttat atcttttaca gccccttttt tgcgtactta agctgatttt aagttttgac
    12241 ccttagtctg gatctacgag gtgagagtca agaatgaaaa aaataattgg agcttttgta
    12301 tttgcaatca ttatcattat tatctgtttt caaatagatg tttttggtca aaaaaagata
    12361 gttggacaaa cggtcagcag tacgaacagt acggttacaa agacaaagga atcatttgat
    12421 gatacagaac aagagacatt aatcaaaatc ggaaaaaatg accgtacact atatacagat
    12481 gactcgctaa ctaccccact tgcaactata aacggcggag agctggcgga gtttctttct
    12541 gaaacaaaaa atgcttatca gatcaagaca aatgatggct atactggata tttagccctc
    12601 gaagatggaa ccaaagtcac aaagaatata caaacaaaac cggaagagct atctgatgca
    12661 gtgattgtat tagatcctgg gcatggcgga aatgatactg gggcattaag caatgatgaa
    12721 caaacagaag aaaaagatat aacattaagt acagccataa aagtaaaaaa ggcgctagag
    12781 gatgctggag caacggttta tttgacccat actacagatg aattagtaca gcttggagat
    12841 atatgcgact acagtgaaga aaaaaaggca gatgtcttta tcagtttaca cgcagactct
    12901 acggaatatg cgaatgaagc aactggaatc acgacatatt attactatgg acaagaggaa
    12961 acattagcac agacaattgc tgactccttc actgatctgc ctctggattc aagaggaatc
    13021 tccactggga actatcaagt tcttcgagaa aatcttcaac cttctatttt aatcgagatg
    13081 ggttatatga ataatgatag tgatttaaag gaattagtaa cagacagtta tcagcagcag
    13141 attgctgcaa gtttgacaca agcattgacc acttattttc agtaataaat aagcccagcc
    13201 aaacaaaaat agctcctcat atttttcgtt tggccgggct tattatcgca gatgcaagct
    13261 tttgaacacc gtttaaggaa gaggttgtga gcctgccgtt taactccgcg tcacaaggag
    13321 caatttcaaa aaacagcttc tcatgtttga tgactattgt gacttgtgac cgaggagttg
    13381 gcatgagaac accgtttatt cggaacaagg gactgtgaca tgacttttgt cacagttcca
    13441 ttattttttt agcagcttca aagctttttc tgtattggca aaatgaagtt tggcatattt
    13501 ttcaagcata ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt
    13561 tccagctcct gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa
    13621 aaagctggca cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt
    13681 agtaacgaag tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa
    13741 atttgcttcc ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt
    13801 ttttagcagc aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta
    13861 ttttggttgg atctcattgt atttttctgg agataagaca agcagtttat aggggatggt
    13921 tttttttaag atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct
    13981 aacaccaagt ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt
    14041 tagcggacca aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact
    14101 gatatttttt ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc
    14161 cccccattta tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt
    14221 ataagcggta atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt
    14281 atttaacaaa taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt
    14341 gataatgacg ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc
    14401 acgacttttc gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata
    14461 atagttaagt atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca
    14521 ctaaataaaa ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc
    14581 tacaaagcag tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat
    14641 atggatatcg taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca
    14701 caaatggata atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg
    14761 aagaaacaag aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg
    14821 atcaaattta cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct
    14881 cgggaagtat tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca
    14941 caacagattt taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat
    15001 ccttttatca aaataagaaa aatttaaaaa agttagaaga gaaaggaacg tagcatgctg
    15061 agtttgctga ttttattttt attgctaatt gcgttttttt ctggagcacg tcgcgggttt
    15121 gctttgcaaa tcatattcgc gattggatat gtattgtcat ttattgctgc ccagcatttt
    15181 tacaaaccat tagcaagtca tttggaatta tacattccat acccagctgt caccccaaca
    15241 tcaaagttag cattttttga tcaggtattt gcttttcacc tagatgaagc tttttatgca
    15301 ggaactgctt ttctgattat tttgctaatt ggctggctgc ttacccgatt tgtgggggtt
    15361 tttgtccatg gattgacgta tgtgccgatc ttgagacagg tagactggat tgcaggaggc
    15421 atcctgtcac taatcatggc ttacgttact atatttttga ttttgcagtt acttgccttt
    15481 gttccattag atgtggtcca aaatcaattc ggcgagcata gtttcgctcg ttttatcgtt
    15541 gagagaacgc catttcttac caacaagatg tatgagcttt gggtaacgaa tattttgaat
    15601 taaggaaaat agtgctggaa aatatttcgt tttttgtaca gagtagttta tttaagaagg
    15661 ctgcgtagcg tatgtccggc acagctcttt tttcatagtg aaagataagg tgataaaatg
    15721 aataaccgaa tccttgaaac attggaattt gaaaaagtca aacagatggt cagacaattt
    15781 gtagtgactg cacaaggaaa agaagaactg gcagaattgg taccagtgag tgagaaacaa
    15841 acgatcacaa actggcttca agaaactgag gatggattga aagtgcaacg tctgcgaggc
    15901 gggatcccta tacctaaact tgaaaatatc cgtccccata tgaaacggat cgagatcgga
    15961 gcagacttga atggagtgga gcttgcacaa gtttccagag tattgtctac gacaagcgaa
    16021 ttgaatcgtt tcattgatga tctttctgac agtgaaatcg aatttgcacg tttgtatatg
    16081 tgggcagatc agttagtcac gatccctgtt ttgagccgcc gtttaaagga agcaatcgat
    16141 gaagacggac gcgtgactga tgacgcttct cctgagttaa agagcataag acagaacatt
    16201 cggcgaagtg aacaagctgt tcgtgaacag ttagatggga tcgtccgtgg gaaaaatgca
    16261 aagtatttaa gtgatgcgat catcaccatg cgaaatgacc gctacgttat cccagtcaaa
    16321 caagaatacc gcggtgtatt tggcggtgta gtccacgatc aaagtgcttc aggacagact
    16381 ttatttattg aaccaaaaca agtggtggat ttgaataatc gtttgcgtca ataccaaatc
    16441 gctgaacgaa atgagattca acgtatttta agtgaacttt ctgctgaact tgttccccat
    16501 cgtcaagaaa tcatccacaa tgcttatgtg attggtaaaa tggatctgat gaatgcaaaa
    16561 gcacgatttg gtaaagaagt aaaagccatc gtgccgggaa tcagtgaaga caatcatgtc
    16621 gtcttgaaac aagcaagaca tccactgatc gatcaggaaa aagtagtttc taatgacatc
    16681 acaatcggaa aagactatca agccatcgtc attacaggac ctaacacagg tgggaaaacg
    16741 atcacgttga agactttagg tctgttacaa ctgatggggc aagcaggatt accgattcct
    16801 gcaggtgaag aaagtcaaat cggtattttt gaggaagtat ttgcggatat aggagacgaa
    16861 cagtcgattg agcaaagtct atcgaccttt tcttctcata tgacgaacac tgtagatatt
    16921 ttatcaaaag tcaatgaaaa aagtctggta ttatttgacg agctcggtgc aggaacagat
    16981 ccgcaagaag gtgctgctct agcaatcgca atcttagatg atcttggtaa aaagtctgct
    17041 tatgtgatgg caacaacgca ttatcctgaa ttgaaagttt atggatacaa tcgggcaaat
    17101 acgattaatg ccagcatgga gttcgatgtg gatacactaa gtccaacgta tcgtttgctg
    17161 attggcgttc ctggacgaag caatgcgttt gaaatctcta gccgattagg tcttgataca
    17221 gaagttattg atgaagcgaa gcagttgatg aatgatgaga gccaagattt aaacgaaatg
    17281 atcacagatt tagagaatcg ccggaaaatg gcagagaccg aatatctgga aatgcgtcat
    17341 tttgtttctg aggcacaaga acttcacgat gatttgaaag aagcttacag ctatttcttt
    17401 gaagaacgtg aaaaagaaat ggaaaaagca aagaaaaaag caaacgaagt cgtgtcagag
    17461 gcggaagaaa aagcagaaaa aatcattgct gatattcgta aaatgcaaca gcagattggg
    17521 caaggaaatg tgaaagagca tcagttgatc gatgcaaaga cccaattagc aaatctacat
    17581 caagaagaaa ctttgaagaa aaataaagta ttgaagaagg cgaaagaaca aaaaacgttg
    17641 aaaccgggag acgaagtgtt ggttacgact tacggccaaa gagggacgct tttacgtaaa
    17701 aacgggaatc agtggcaagt agaaatcggt atattaaaaa tgaatgtgtc cgaagacgag
    17761 ctgacgcctg ttgctcctca aaaagagccg acacaacgag ttatccatgc tgtacgatct
    17821 gaatctagta gtcacgtgcc gaatcaattg gaccttcgag gaaaacgata cgaagaagcg
    17881 ctaagtgagg tagatcaata tttggattct gccattctag ctggttatcc tcaagtgact
    17941 attgtccacg gaaaaggaac aggcgcactt cgcaagggaa ttactgatta tcttaaaaac
    18001 catcggagcg tcaaaagctt tgagtttgca ccggcaaacc aaggcggaaa tggtgcgaca
    18061 attgttaaat ttaagtaaag cttactaaag gtaagcagat ggttcgctat ccaagtatag
    18121 aaatgctata ctaatcatag attttaaaaa tggaggtctt tgatatgtca caagtaatta
    18181 cagataagac atttaatgaa gaaacagata aaggacttgt attgattgat ttttgggcaa
    18241 cttggtgtgg tccatgtcgt atgcaagcac cgattttgga tcagttggaa caagaatatg
    18301 atgaagaaga attcagaatt gctaaaatgg atgtggatga aaatccagaa actcctcaac
    18361 aattcggtat catgagcatt ccaacattaa tgttgaaaaa agatggtcaa gtagtagaaa
    18421 aagctgttgg tgttcattca aaagaacaac tacgtcaaat gatcgatcaa tatttataaa
    18481 aacttacagc aaaaataagc tgagggtggg actgaatcca gttccgtcct cggttttttt
    18541 gcggttgagt taaggttatt caaattaata ttttttgagt ataattacgt tgtcaggctg
    18601 gattgttttt ttgacagaca tgctataata actttaattg aatatgaagg ggagtaccag
    18661 atgaacgaac gaatcaaaaa caaattagct ttacttcctg atcagcccgg ctgttacttg
    18721 atgaaagata aaaatggaac gatcatttat gtcggcaaag ccaaaatatt aaaaaatcgt
    18781 gtccgctcat attttactgg cagccataat acgaaaacgg aacggcttgt tagtgaaatc
    18841 gaagactttg aatacatcgt tacagaatcg aatatcgaag cattgctctt agagatcaat
    18901 ctgataaaga aaaatgatcc taaatacaac attatgttaa aggatgataa aacctatcct
    18961 ttccttaaga taacgaatga aaaatatccc cgtttggtga ttactcgtaa agtactaaaa
    19021 gacaaagcac tgtattttgg accatatccc gatgtcggtg cggcgaatga gacaaagaag
    19081 atcctcgatc gattatttcc tctgagaaaa tgcaaaccta gtcagacgaa ggagccttgt
    19141 ctttactatc atttgggaca atgtctgtgt ccttattatt tcgacgtaga tccgaaagtc
    19201 tatacgaaaa tcgttgatga agtgaaaaag tttttaaacg gaggacacga aaaaatcgag
    19261 tcagagatcc atcagaaaat ggaaaaagct gctgaaaaca tggagtttga aaaagctgca
    19321 gaataccgag accagatccg tgccattgag acaatcatga ctcgccaaaa aatgacaaat
    19381 actgatttac tggatcgaga tgtatttggt tatgcgattg acaaaggatg gatgtgtgtc
    19441 caagtgtttt tcgtaagaca aggaaagcta attgaacgag atgtttcaat gtttcctttc
    19501 tataacgaag cagaagaaga tttcttgact tacctcggtc agttttatca ggaaaatgag
    19561 catttcattc ctaaagaagt cttgattcct gatacgatcg acaagcccag tgtagaagct
    19621 ttgttagcta cgaaagtgct gcaaccaaaa cgaggagaaa agaaaaaatt agttgaattg
    19681 gcgaataaga atgcctcagt tgctttgaat gaacggtttg atctgattgc cagaaaacag
    19741 gaacggacga tcggagcagt tgaacgttta ggaaacgcga tgaatatccc gaccccaata
    19801 cgtatcgagt cttttgataa ctctaatatc atggggacga atcctgtggc tgcgatggtc
    19861 gtattcattg atggaaaacc ggcaagaaaa gaatatcgga aatataaaat aaaaacagtc
    19921 aacggaccgg atgattatgc atcaatgaga gaagtcatct atcgcagata ttcacgagtg
    19981 attcgcgaag ggttgccttt acctgatttg atcttgatcg atggaggaaa aggacaagta
    20041 gatgtggcaa aagatgtctt agaaaatcaa ttaggaatcg atattcctat tgctggcatg
    20101 gcaaaaaacg ataaacataa aacaaatgag ttgctattcg gtatagattt ggctgtagta
    20161 ccgcttgagc gaaattcacc agagtttttc ttactccagc gaatccaaga tgaagtccat
    20221 cgattcgcaa tcactttcca tcggcagcta cgcagtaaaa acagttttgc ttcccgatta
    20281 gatgaaattg aagggttagg accaaaacga aaaaaaatgc tgcttaaaga gttcaaatcg
    20341 ttgaaaaata tcacagcagc cacgacagaa gaattacaag aaatcggact gccgaaaaat
    20401 gtcgcacaaa acgtatttga taaattacat caggaatgag gaaagggcac tttacaaaga
    20461 aagtaaagtg tcctatcatt ttatgtacaa caaaaagagc gtttacgttt tgttggtaaa
    20521 taataaatcg cttacgtctg gtgtctaagg atatatgtga tttcttgaag aaggagtgaa
    20581 gaaatgttag aagtcaagga tcttgttaaa acttttggga actacacagc tgtcgatcat
    20641 gtctctttcc aaatccccga tggaaaaatc atggggctaa ttggccaaaa cggggctggg
    20701 aagacaacga cttttcgttt gatcttggat tttttgacac aagaccaagg agaagtgtta
    20761 tggaatggcc atcatcttgg ggagaaagat tatgatgtca tcggttatct tccggaggaa
    20821 cggggacttt atccgaaagt aacgattcaa gaccaactgc tttattttgc agaacttaga
    20881 gggaaatcaa gaaaagagat tgaaccaaaa atcgattttt ggatggaaaa atttcaggta
    20941 aaagggaaaa agacggataa agtaaaatct ttgtctaaag gaaatcagca aaaagtccag
    21001 ctgatcgcca cgttaatcca tgaacctaaa ttgatcattt tggatgagcc attcagcggg
    21061 ttagatccgg taaatgctga attgctaaaa gatggaatat tggaattgaa aaaacatggt
    21121 tcatgtgtca ttttttctag ccacaatatg gataatgtag aaaaaatctg cgatcatttg
    21181 atcatgttgc gaaacggaaa aatggttttg aatggaaaag tacatgagat tcgtgaatca
    21241 tttggccgta cgaagttatt cttggaatct ggtttaagcc aacaggaagt agaagaaatc
    21301 gaaggcgtga aaaaagtgat ccttcatgaa gacaatacgt tagagattac cttagaagat
    21361 cctgaagctg ggaaagaaat ctttactcgt gcgactcagt tcggctacat tcctatgttt
    21421 aaccagcaac cgccaacttt ggaagagatt ttcaaattga aagcaggtga aatcgatgcg
    21481 taagttttgg attattacca aagatgtcta tttaaaaaat gttaagtcga tttctttctt
    21541 gatcatgata ttagtccctt ttatcatgct gggtatcatt tatgtagcag gaaactttgc
    21601 tcaacaaaat agtgatgtca ataaaatcgg cgtattggct gataatgagc aaatcagtca
    21661 gcaaatgagc caagtcaaaa cagatgattt tgagttcaaa gcagttgatt ctgaagaaaa
    21721 agcacaaaaa gagctttcgg aagaaaaaat cgatgcgtat ttagtagtct caactgagaa
    21781 tgataagata aatgggatgc tttatagcga aaattcatta ggacaaagta cacaactact
    21841 gatacaacaa caactgactg gcttgcaatc gatgcttcga gcaagtcaat taggcatttc
    21901 accagaagaa gtagctagct tgagccaaca agcaagtttg actcgacaaa aagtcaattt
    21961 tgattcaaaa ggaaaaatga cagttggaga agataactcg acagtccaat atgtcgtaag
    22021 ttatgtagca acgattgtct tgttcattat tattttgacg tatgcgcaga tcattgcaca
    22081 agagatcgct tctgaaaaag gcacgcggat catggaagtg atcctgtcca gtacgactgc
    22141 acaaaagcat ttttacggaa aactaacagg tgttttactt gttgcattaa cgcaaatggc
    22201 attatacggt attatttttg gtataggata cagtcaattc aaagatatgg aaatggtcaa
    22261 aaatatatta gacggtatct cattggataa cttgattgga ccattcttgt ggtattctct
    22321 tctattcatg atctttggca ttttgatttt ttctgtttta gcagctttat gtggctcttt
    22381 ggtcaacaaa gcagaagata cagcaaaagc aattttgcca gtgacttatc tatctctagg
    22441 cggatatatg cttggattga tactaggagc ttcagatcca aacaatatcg ttattcggat
    22501 cacttcgtat attccattct tatcttcata tattatgccg gttcgcttag caaatgagac
    22561 agtcggtatg ggtggagcat tgatttcctt gttggtactt gtagccgtaa caatcttatt
    22621 gatgatcttg tctgcaaaca tgtacaaatc gaatgtactc gtgtatagcg aaggcggatt
    22681 atggagttca ttgaaacaat cgatttcaat tatgagaaat gaacgaaaaa aagcataaaa
    22741 tatagaaaaa tatagaagga agctggacat ttattgttcc cagcttcttt tttgtataga
    22801 cctatttcta tttttttatt atattggata tatatattca ttgaaaatag agcataacaa
    22861 ctttataatg ttgagtatac caatttttgg aggcgtttta atggaccgct atttagattt
    22921 actttcagag aagttcccta caacggaaga tgtcatcacg gaaatcatca atctcgaagc
    22981 aattttgcag cttccaaaag gaacagagtt atttttgagc gatatacatg gcgaatttcc
    23041 tgcttttgat catattctgc gtatcggttc aggaaatctt aaagaaaaga tcaaagacct
    23101 tttctctgaa caaatgacgg aaacggagat cagccaattc actattttta cggcttatcc
    23161 agaatatgtg ttgacgactg attggtacaa aaaacaagat aaatcccagc tgatccatcg
    23221 tttgatcgat cttttacggt ttacgacagt caaatacact cgatcaaaag ttcgaaaagg
    23281 attgccgaaa gaatatagct atatcattga ggaactcttg tatatcgatg atcgtatcaa
    23341 cgggaaaaaa gactatgtaa aaaaaatcat tgaacaactt gtcttgatga aggaagaaga
    23401 aaaattttta acaaaacttg cgcagacgat ccaaaaatct gtcatcgatc atttgcatat
    23461 cgtgggtgat atttttgatc gaggaactca agcagccaaa gtaatggatc gaatcatgga
    23521 tttttcctct gtagatatcc agtgggggaa tcatgatatt ttgtggattg gtgcgtattg
    23581 cggctctgag gcatgtttat tgacgctttt acggattgcc gcacgctaca actatttatt
    23641 tgaattagag aaagaatatg gattaaattt acgtccatta ttttcttttg cccatcaaac
    23701 ctatcagaag aatccagtat ttacccctaa aaacaggaaa gatctatctg aaagagagca
    23761 agaagaatta gaaaagatcc atcaagcact ttctatcctc caattcaaat cagaacatca
    23821 gttgttgaat cgacgacctg aatttaaaat ggacgatcgt aaattgttgg aaaaaatcaa
    23881 ctatgaggaa tcaacgatcg ttttagaagg tcagctctat gagttgaaag ggacttgttt
    23941 tcaaacgatc cagccagatg atcctaaaaa attcttggaa gaggaacaaa aagtgatcga
    24001 tagtttgatg tattcattcc agcactcgtt aagaatgcaa aaacacatga cgtttttgat
    24061 cgaaaaaggg ggaatgtatc tcacgaacaa tcagcatgta ctgttccatg gctgtatacc
    24121 tgtagatgaa aaggggcagc tcttagcatt tgaattagac caatcttatc gcggaaaaga
    24181 actgctggga ttttttgaaa aacagataac tgaaagcgca aaagatttga ctgcaaatga
    24241 agatttggcg accgatttga tttggtatgc gtggagcggg ccattttcgc ctttattcgg
    24301 caagagtaaa atggcaactt ttgagcgcta ttttctcaca gagcctcata cgcacgtcga
    24361 aaaaagcaat ccctattatc acttgcgaga tgaagagtgg ttcagtgaaa aaattttggc
    24421 agaattcgat gcaaaagaag aaggttcagc aatcatcaat ggacacacgc cagtaaaagt
    24481 aaaaaaagga gagtcaccga tcaaagcaaa cggaaaagca tttgttattg acggaggtct
    24541 atccaaagct tatcaaaaaa caaccgggat tgctggctac tcattactat gcaattctta
    24601 cggtttccaa atcgtgacac attatccgtt cttgcctatt gaacaacaat ttgcaaaaaa
    24661 atctgatcaa acgaatgtaa aaaaagtgat cgagcagcag ctcccacgca aacttaatcg
    24721 gaatacgaca aaaggaatgg aattacagca acagattact caactccaaa ggctgttgga
    24781 ttatcgaaag gacgattgag aaaaatgcta gccaacattg ctcaatagac taaaaagcga
    24841 ctgcaacaaa aggcgtgtcg cagtctcctt cggaattagc tcaaaaaacg gtaaaatatt
    24901 agaagctatt tttctgttta tttctctaat actcaggtca gggcgctgat ttcacgacct
    24961 cttccttaaa cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccaacaaac
    25021 atgagaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc aggctcacga
    25081 cctaatcaga aaaacgatta aaattcattc acaaagaaat cgaataaacg ctgttcagat
    25141 gaatctacac gatgagaaag ttccggatgc cattggacac caaggatttt gtgcatatta
    25201 tctttacttt ggactgcttc aatgagtcca tcgttggaaa aggcgatggc ttttaacaaa
    25261 ggagaaagat ctttgactgc ctgatgatga taggaattca caaagtatga atcagggaga
    25321 aggtcactta ataaactgtc cgaaacgatt ttgacttcgt gagttgcaaa ctgcggggca
    25381 gtgggctgtt gttcgtgttt tacggaccat tttggatata atgaaagatc ttgataaagt
    25441 gttccctcca acacgacatt caatagttga agtcctcggc agactgcaaa aatcggtttt
    25501 ctttgtttca atgcttccaa gatcaatgcc tgttcgaatt ggtctcgttg aatatttgtt
    25561 tccgtcagtt ttggatgagg gtcttccatg taaagttgcg gactaacatc ctgaccgcct
    25621 gcaagaatga gtttgtcgat tttctcaatt agttgaggcg ctgaatctgg agacatgact
    25681 ggcagaataa gaggtaatcc ttgagcatct aaaactgcat ccacaaatcc ctgcggtgta
    25741 tagcttactt gatttccttg gaaagtatct gtcgctcgaa tcagttgatt cccggcaatg
    25801 ccgatgattg gtttcatttt tattccccct aaagtagact aattgtaaca aggatttaaa
    25861 aaaatttaaa ctatgtaaga cttgccccta tacttatctg ttcttgctgt gttactatct
    25921 gtaaagagaa ttttcgtgtg aaattcatga cgatagttga aaatattgct taatttgtta
    25981 taataaaaag taagcagaaa taaaaaggtt gggcgtaagc ttcaaggatc tagttcttca
    26041 aggggtgaga aggacatttt attgaagggg cctgcctata ctaagttatc tttagaggta
    26101 catactttca atgaaaaaag aaagcatgta cctctttttt aaatcgaaag gaagtgaagc
    26161 aagtgttaga agctttggaa caaatccgaa aagcagaaga agaaaatgag aagcgtgctc
    26221 gaaccctacg gcagcagttg aagacctatg cagaagaaaa agagcaagaa ttggagataa
    26281 tccaaacgaa cagtcggcaa cagctgaaac agttggtaga agaaaaagaa caattcgaaa
    26341 agcaagaatt agaaaaagaa aaaatgaaat tgaaagcaga agcagacaag gtgcaagcaa
    26401 ctttgaaaga ccaatatcaa aagcatcaga gtcaagcaat cggtgcaatc attgaaaggg
    26461 tgaaagaaac ttatggccgt cactaagatg gaaaaagtga ccctcatttc agataaaaga
    26521 aatcaggaga ggattttaca agcagtacaa ggaatccaaa atgttgaaat ccgggatctg
    26581 tatcaaaata gaacaaacaa ccagtgggta gaaaaatatt ttccagatac cacgattatt
    26641 gataaagaag caaaattaag cgctttaggc aatcggctga aagacatcca agaagctatc
    26701 caattcatac aacaccacgg cgatagccgt caaaagaaac tccatttgaa acatcaagaa
    26761 ctttccttac aagacttgga agcaaattat tccgaagaaa atttcatgaa taaattagaa
    26821 gaagtccttg atttaaaaga aaagtgggaa cagctaactg aaaaacgtga tcgtttagaa
    26881 gaagaggaag aatggcttct tcattggcaa cagctggata ttgctccaag tatgtatgca
    26941 agtaagacga ccatttttga gatggggact gtgagtgcag caaattttga aagtttcaag
    27001 gaagaactaa gtaagcttga tgacgtttat atggaagaaa tagattatac gccaaaacaa
    27061 gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg ctatagaaga aattgctggg
    27121 aaatacgggt tcaataaaga aacgtatcct tatgaacaaa atcctaaaga aaaattgcaa
    27181 gaaaccaaac agcaattatc cattgttcac gagcagcaaa aaaagataac gacagcgtta
    27241 ggtcagtgca gcggctatat ccaagaattt gaatgggtag aagaagtcac acttgccgtt
    27301 gcagaacggg aaaaagtaaa agaacggttt atccatgcca cttatctgat cgttttgcaa
    27361 ggttgggtag atgcagaaga aaaacagaat ttgatgcatg tgttaaacga aactgtatca
    27421 gaagaagata tatacgttag tttcgaaaca ccaaactcag aagagattca gcaggaagtc
    27481 cctactaaat tgaaaaacca tccgcttatc gaaccttttg aattgctgac tgaaatgtac
    27541 agtctgccaa aatatgaaga agtagatcca acgccttgga tgacaccatt ttatcttgtc
    27601 ttcttcggga tgatggtcgc agatgttggt tacggattgc tgatgctcat tgggacaata
    27661 ctagctcaaa aactatttgt tttaccccgc gggatgaaac gtttcgtaaa attctttgag
    27721 attctctcgg taccatcgat catctgggga ttgatctaca gttcattttt cggacaggca
    27781 ttaccaaaag aactatttgg tatccgcttg cctttcccaa tcctatctac gacggatgac
    27841 gtcaatacga ttttgatttt atccgtgatt ttcggtctga tacagatctt ggttggatta
    27901 ttcgtggcag caaaggaaaa tatcaggagg aaagattact tgagtgctgt cagtgatggt
    27961 tttgcctggc aagggatcct gattgggatc gttattgcat tgattggtgt catgttgata
    28021 aaaaatacag catttgttta tcttggcgcg gggctatcga tcattgctgc tttatgtatc
    28081 ttaatcgtgc cgattatcca atcgccgtct aaagtcaagg gtgctgccaa aggagcgtat
    28141 aatctttacg gattgactgg ttatatcggt gatttagtca gttatacacg tttgatggca
    28201 ctaggtattt caggcggaag tattggcgcg gcattcaata tgctagttgc tttcatgccg
    28261 ccagccgctc gattcagtgt agggatcctt ttgatcatcg ctttacacgc actgaatatg
    28321 tttttgacac tgttaagtgc ctatgtccat ggtgcacgat tgcagtatgt cgaatttttt
    28381 ggaaaatttt atacaggagg cggacgtgcc tttgacccgc tgaaaacggc ggaaaaatac
    28441 gtcaacatca atcataagaa aaaaaacaat aaataattgg aggattttta tacaatgatg
    28501 gattacttaa ttagtcaaaa tggtggaatg gtatttgcag tattagcgat ggcaacagcg
    28561 acgatttttt cagggattgg ttcagctaaa ggtgttggaa tgacaggtga ggcagcagca
    28621 gcgttaacaa caagtcaacc cgaaaaattc ggacaggctt tgattctgca attgcttcca
    28681 ggtacgcaag ggttgtatgg attcgttatc gcctttttga tttttatcaa cttaggaaat
    28741 gatatgtcag ttgtacaagg attgaacttt ctaggcgctt ctctgccgat tgcttttaca
    28801 ggtttgtttt caggtatcgc ccaagggaaa gttgcagcag caggtatcca aattttagcg
    28861 aaaaaacctg aacatgctac aaaaggaatc atttttgctg cgatggttga aacatatgcg
    28921 attttaggtt tcgttatttc cttcctatta gtattgaatg cctaagcata aaggaggaaa
    28981 agcagatgaa cgccattgaa aaaatcatct ctcagatgaa tgaagcagca gagcaagaaa
    29041 gagcggcgtt agagcaagaa gagcgaatga agatcgatca aaactttgaa caaaaacgta
    29101 cgcaaatcga aacagaacat caaaaacaaa aagaaaaaca aatcgaatta cttgaaaaaa
    29161 aataccgtca gcttaggaat cgccaacaag tagaagtacg acaagaaaat ttgaatgcga
    29221 agcaggaatt tttacgacga ttgtttgcag atgcaatagc tgaaatggag aattgggatg
    29281 aatctgaaca aatccagttt atcaaaaatt cattgtattc attacctttg acaggcaaag
    29341 taacattcat tgctggagaa aaatcagcag cttatctttc tcagacattg ctggatgaat
    29401 ggaacaatga acttcccttt atgatggttt taagtgatga aactgttgct gatcaagcgg
    29461 gttttctgat aaacgatcaa ggagttcagt ataattttct tttcagcagc cttgtacaag
    29521 atatccaagg aacgatgagt tttgagattg ccaatcaatt attcgaataa ggagggatca
    29581 gtgtatggaa taccacgaac taaatccctt gattcgcgga agagagcttg aacttttgtc
    29641 caatgagaca tttgaacgga tgattcaaac aaattctgtc gaagcacttg gtgaaatatt
    29701 gaaatccacg atttacagtc catatattta tgacggatat gaatatgact tcgaacagaa
    29761 tcttttaaag gagcagagcc gtctatttcg ctggttaaag gaatcagcac cggagcctga
    29821 aatcgtttgg atctatacga tgcgctatac tttccataat ctgaaggttt tgacaaaagc
    29881 cgagatgaca aatcaaaatc tggaccattt gtatatcgac gatggatttt attcattaga
    29941 aaccttgaag gatgccattc atacgcaagt ttcaaatgaa ctccctgaaa gcattatgga
    30001 cagtatacga gaagtacgtg attattttga agagtctacc attcttcagg gaattgacgt
    30061 gatttatgat cgacagtttt tgacagagca gcgacggctt gccgataagc taggatatca
    30121 agaattgctg acagaagtca ttgcttttat tgacttgacc aatatcacaa cgatggctcg
    30181 aggaatattg cagaaaagaa catatgggtt catgacgacg gtactttcca gctcgggaag
    30241 tatcgaaaaa gaagtatttt tgaactttgt agataaagat atggaagatt acacgaattt
    30301 tctattagag acagattatg ctgattttct gcgccctgtc atccgtgagg gaaatcttga
    30361 tctcgtaaag cttgaacagt tgaaagatga ttatctaagt tcattgtatc aagaagcaca
    30421 aacccaggct ttcggaccac tgccactact agcctttttg aacgcaaaag aggtagaaag
    30481 caaaaacctt cgtttactgg ttatcggcaa aagaagccat ttcgaaccag aaacaatcag
    30541 aaagcgggtg agacaagtct atgacgcata agatcggtgt agttggcgac aaagattctg
    30601 ttttgccatt caaattgttt ggctttgatg ttcgttatgg tacaaccaag caaaccgttc
    30661 gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt gatttatgtc acagagcaat
    30721 gtgcagcatt gagcccagaa acgatcgaac gttacaagga acaaatgata cctgctatcg
    30781 tattgattcc caatcatcaa ggatcattag ggatagggct ggaagaaatt caaaaaaacg
    30841 tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa ggagatagaa attttgcaaa
    30901 ttggaaaaat cataaaagtc tccggacctc ttgtaatggc tgagaacatg tctgatgcca
    30961 gtatccaaga catgtgtcta gtgggagatc taggagttat cggagaaatt atcgagatgc
    31021 gtggcgatgt ggcgtctatt caagtatatg aagagacttc tggaattggt ccaggcgaac
    31081 cggttcgctc tactggtgaa gctttatctg ttgaattagg accagggatc atttcacaaa
    31141 tgtttgacgg aatccaacgg ccattagata cctttatgga aatcacacaa agtaatttct
    31201 tagggcgcgg agttcaattg cccgcattgg accatgagaa aaaatggtgg ttcgaaccaa
    31261 cagtagaagc cggagaaacg gtgagtgctg gagatgttat cgggatcgta gaagaaacaa
    31321 aagttataaa acataaaatc atggtgccaa atgggataaa aggaacgatc aaaacaatcg
    31381 aaaaaggttc attcactatt gatgaaacag tatgtgtcat tgaaaccgaa aatggtgaca
    31441 aaaatttgac gatgcttcaa aaatggccag taagacgtag tcggccaatc aaagaaaaat
    31501 taaacccgga tgcacccatg atcactggac agcgagtgat tgatacattc ttcccagtga
    31561 caaaaggagg agcagcagct gtacctggtc cttttggtgc agggaaaaca gtcgttcagc
    31621 atcaaattgc aaaatgggcc gatgtggaca tggttgtcta tgtgggatgt ggcgagcgtg
    31681 gaaatgagat gacggatgtt ttaaatgaat tccctgaatt gattgaccct aatacaggag
    31741 aatcgttgat ggaacgtacg gtattgattg ctaatacatc aaacatgcca gtagcagctc
    31801 gagaagcttc tatctatacg ggaatcacaa ttgcggaata tttccgtgac atgggttatg
    31861 acgttgctat catggcagac tccacttcac gatgggcaga agcacttcgt gagatgagcg
    31921 gacgtttgga agaaatgcct ggtgatgaag gttatccagc ttatcttggt tcacggcttg
    31981 cggaatacta tgaacgatct ggaagagtca ttgcattagg atcagaaggt cgcgaaggaa
    32041 gtataaccgc aatcagtgct gtatccccgt ctggagggga tatttcggaa ccagtcacac
    32101 aaaatacatt gcgggtcgta aaagtattct ggggtcttga ttccagttta gcccaaaaaa
    32161 gacattttcc ttcgatcgat tggatacaaa gctattctct ttattcaaca gaagtaggga
    32221 agtatatgga tcatatcctt caagctgact gggcatccat ggtcaccgag ggcatgcgta
    32281 ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt ggtagggatt gattcccttt
    32341 cagataatga ccgtctgaca ttagaagtgg caaaatccat tcgtgaagat tatttgcagc
    32401 aaaatgcttt tgatgatgtc gatacattca cttcaagaga gaaacaattc aaaatgttga
    32461 agatgatctt gacctttggt gaagaaggac gccgtgcgtt gtcgttaggt gcttatttaa
    32521 gtgagatcat ggacggtacc gtatcgattc gtgaaaggat cggacgaagc aaatatatcc
    32581 cggaaacaga attagaaaaa ctagatcaaa ttattggtga aatcaagcaa acgatgcaaa
    32641 cgatcgtttc agaaggaggc atgagcaatg attaaagaat atcgtacgat cggtgaagtc
    32701 gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca aatatgaaga attaatcgaa
    32761 gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat tggaagtcca agaagataaa
    32821 gcaatggtcc aaatattcga aggaacaagt ggtatcaatt tacgtgactc ttctgtacga
    32881 tttttaggcc atcctttaga actaggtgta tcggaagaca tgatcggtcg agtattcgat
    32941 ggtttgggac gaccgaaaga taacggacca gagatcctgc ctgaaaagca tttggatatt
    33001 aacggagaag taatcaatcc agtagctcgg gattatccag atgagtttat ccaaacaggt
    33061 atctctgcaa tcgatcattt gaatacatta gttcgtgggc aaaaattacc agtattttct
    33121 ggctctggtc ttcctcataa agaattagct gctcagatcg cgcgacaagc aacggtactc
    33181 gattcatcag atgatttcgc cgtagtcttt gctgctattg gaatcacttt tgaagaagct
    33241 gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg accgttctgt catgtttatg
    33301 aatttagcaa acgaccctgc aatcgaacgt atcgctactc ctcgtatggc tttgactgct
    33361 gcagaatatc ttgcttatga aaagggaatg catgttttgg ttatcatgac agacatgacc
    33421 aactatgcag aggcattgcg agagatttct gcagcacggc gagaagttcc aggaagaaga
    33481 ggatatcctg ggtatcttta cacgaactta gctactttat tcgaacgagc aggaagaatc
    33541 cgcggactaa aaggatcggt tactcaaatt ccgattctaa caatgccaga agacgataaa
    33601 actcatccaa tccctgactt gactggttat attacagaag gacagatcat cttgactaga
    33661 gagctttaca agagtgggat tcagccacca attgatgtac tcccgtctct ttcgcggtta
    33721 aaagataagg gaactggacc cggaaagaca agagaagacc atgcagctac gatgaatcag
    33781 ctttttgcag cctatgcaca aggaaaacag gcaaaagaat tagccgtagt tttaggtgaa
    33841 tctgctttat ctgatgtaga caagatctat gcgaagtttg ctgagcggtt tgaaaaagaa
    33901 tatgtcaatc aaggattcta cacgaaccgt tcgattactg aaacacttga tctagggtgg
    33961 gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa tcaaagacga catgctggac
    34021 aaatatctgc cagaagggaa gtgagttctg atggcacgat tgaatgtgaa tccgacccgc
    34081 atggaattga ctcgattgaa aaaacagttg acaactgcca caagagggca taaattatta
    34141 aaagataagc aagatgaact gatgcgtcaa ttcatcctgt tgattcgaaa gaacaaccag
    34201 ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt ctgattttgt attggcaaat
    34261 gcttcgatca cggaaccttt catcgaagaa ttatttgttt tgccggcgga aaacgttgat
    34321 ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac ctgtgatgaa ttttgaatat
    34381 gatgaacagc tgacagacac acctttggaa tacgggtatt tgaattccag tgccgaactg
    34441 gatcggtcaa tcgatcggtt tactaccctg cttccaaaac ttttggaact gacagaaatc
    34501 gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga cacgtcggcg agtcaatgca
    34561 ttagaatata tgacgatccc tcaattagaa gaaacgatct attatattcg tatgaagctg
    34621 gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag taaaaaatat gggtaccact
    34681 gactaagtgg tatctttttt taaaagagga aaacgatgat taaacaaagc aaacaaataa
    34741 agaaacgttt gccggctgtt cagcttctcg cattaggatt ttttctcctg attttagttg
    34801 gcggcggcct acttacccta ccatttttta gtaatagtgg gcatgggaca aattttatcg
    34861 atgcgctttt cactgcaact tctgctgtct gtgtaacggg attgacaaca ttgaatacag
    34921 cagagcattg gaacagtgct ggtcaatttt tgatcatgct tttgattgag atcggcggtc
    34981 ttggttttat gatggttccg attctatgct ttgcattgat aaagaagaaa gtcagtctca
    35041 gcacacgtat tatattaaaa gaagcgctga atctagaaga agtatcaggt gtgatgaagc
    35101 tcatgcttta catcctaaaa ttggcctttg ttatccaatt gattggtacg gttgctttat
    35161 cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat ctggtacagt ttgttccatg
    35221 caatctctag tttttgtaat gcagggtttg atttgttagg agacagttta gccaatcacc
    35281 agcaaaatgt ttatttgatc atggtggtat ctgctttgat catttctggt gggttaggct
    35341 tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa gaagatgacg attcattcaa
    35401 aaatcgcatt aaccgttaca ggtacccttt tattaggcgg attcgttgtg ttctttttca
    35461 cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt tcaacgattg gcaaacacct
    35521 tttttatgag tgtgacacct agaacggctg gatattattc agttgattac ctccatatga
    35581 gccatgcagg tctgatttta acgatgtttt tgatgtacat cggcggaaca tctgggtcga
    35641 cggccggcgg attgaaaaca actacgttag gtgtgttgct gatccagatg cgttcgatgt
    35701 tgaaaggacg tacgagagcg gaagcatttg gccgtaccat ccgacaaggc gcagtgttgc
    35761 gggcacttac tttatttttt gttacgttga cgttgtgtgt agtcgccatc atgattttat
    35821 cggtgactga aacgatacct aaaacttctg gcatcgaata tattgctttt gaggtcttct
    35881 ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc agacttgact gcaataggga
    35941 aattgatcat tatagcgctt atgtacattg gacgagttgg tgtattgact gtggtcttct
    36001 ctctgatgac aaaagcacaa agaacacaat caaaatacaa atatccagaa gaaagtgtca
    36061 tgattggata atttcatctt ctgacagaaa cctagtgatt tagtaaaata agaaaataaa
    36121 ataatttttc ttgcttcttg gatactttaa gtgcaaaatg ttcgtttatt ttttgaggca
    36181 ctacccgttg ttgaacctcg ggtagtgcct cttttttatt gaaaggaatc aataagattt
    36241 gattccttta tttatcaata ggttataatt catatttttt tattttttct tttacgatac
    36301 ttagtccaga agctaaaatt tcaaggttat tagaggatag ggaaattcga acgaatgcat
    36361 ctggttgcgc acgtgtagta aagcgtctgg aatgataaag tcggataccg ttagcaagaa
    36421 agtcattctc aattatctta gggtcaatct caggatcaac tggaagaatt cgatagtaag
    36481 ggacacgatt cgttggtttt tctactttga aaagagaatc aaaaagctga ttggctcttt
    36541 ttgtaagact taatttagtt tctaagatcc tatttgcttc atttgaatgg aggatttgag
    36601 tgatgacttc cacatctaat ccagaagttt tcacatttat attaaaaatt gcttgttcta
    36661 tcttgctttg gacatgtact ggaactacaa gataagcaac tcgcaatcca gaacaaataa
    36721 attttgtcat tccagctaag tagattgttt ggttaggtaa taaattttgg aaaggaggaa
    36781 tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat atcatcttca ataacccata
    36841 gaccttcttt ttcaatcact tcggctaatt gctctcgtct tttgtgagag atttgaaacc
    36901 ctataggatt attacaagaa ggcataagaa agacaccatg aatcttcttt tttctacatt
    36961 cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa atcaattgga acgatctcta
    37021 aatgatataa ctgagccaat tcaatgaaat tagcgtatgt atatcggtct gttgctattc
    37081 tatcacctgg agagaaaagt gcagaaagtg tgatagccaa tccattttga acaccggaaa
    37141 caatagatag agattctaag gacggaatga ctccttgagt ttttaaccaa tttgctgcag
    37201 tagtcaattg aaaatttgtg cctttgggat cttggtaatt aagcaaacca acaagatttg
    37261 ggttattact agttgattga ataaacggga caacgaattt attacattct tcaaaagaac
    37321 taaccagacc aaaatcaatg atatgttcgg ttacttcatt ggtagagatg gtgatggact
    37381 caaagacatt gggagaatta aatgtgccac ttccaatatt cgtatacagc aaacctttct
    37441 caattccata tttataagcc ttgccaacgg tagtaaaatt taaatctaaa taatcagcta
    37501 gctctctttg ggagggtaac tgagtgtttt tctgtaattt attctttaaa atatcttgtt
    37561 ctaattgttg aatcaacgat cgataataag gacgcttcaa tttttgtttg tctggtcgcc
    37621 aagttaacgg gtaattgtca taagaattaa taggcatttt ctccactcct taaaattgta
    37681 taccatacaa ttttaccatt gattgtattg attttagaag ttaatctttt gtgaggaggg
    37741 aattgtctat gaaaattatt aattttgagg aagtagtagc ttcttttagt tttgaagaag
    37801 cgattcaagt aatgaaaaaa tgtttttttg attatgaaag tggtaaaatc agccaaaatc
    37861 ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa tatgtttgcg atgatgcctg
    37921 cttatttggg ggagaacaga tattttggca caaaagtcat taccgctttt ccggataatc
    37981 atcagtatca tttaccttct catttaggag agattttctt gtttgatgct caaaacggac
    38041 tacccaaagc attgataaac gcaaatgcag tcacctggat tcgaacagct gctgtatctg
    38101 cgctggcaac agattactta gcaaatccgc atgcagaaac acttgcttta attggagctg
    38161 gtcaacaagc attgtcgcat ttgactgcga tttctaccat tcgtcccatc actacagtgt
    38221 ttgtttacga tattttagaa gaaagacgaa atacttttat cgctaaagct aaaaaacagt
    38281 atcctaaaat aacgtttata aatgcaatat cagtagaaga tgcagtacgt ccagcggaaa
    38341 tcatttgtac gttgacctct agtaaagagg catttcttca aaaagagtgg gtaagggaag
    38401 acgcacatat caatgctgtg ggcacattta ctcctgatac aagagagata agtacatcgt
    38461 tgatggaaac ttctcaaatc tatgtagatg attactcagc tgccttaaca gaaagcgggg
    38521 acctgcttat agccatggca gaaggagatt tagaggaatc ttgtattgtt ggtacattag
    38581 gcgaattagt ttctgagaaa aaaacatttt ataaaggaga atcgaggccg acgattttcg
    38641 acgcagttgg tctagctatt gaagatcttt gttgtgcaga atatatttat aacaaattag
    38701 agaaaggggc tttaaaatga acttagatta ttacgtagtt gatgcatttg cagatgaggt
    38761 gttcaaagga aatccagcag cggtttatgt gctagaagaa tggctgccag aaggtacgat
    38821 gcaaaaaatt gccattgaaa acaatttatc tgaaacagca tttacagtga agaaaaacca
    38881 agaatttgag cttagatggt ttacgccaga tcgagaaatc gatctttgtg gtcacgcgac
    38941 attagcaaca gcatttgttt tatttaacta ttacaaaata cctgatgaaa cgattaaatt
    39001 ttctactcaa agcggtaatc tatttgtgac aagaattaaa gattattatt acatggattt
    39061 tcctagtatt atgccgaaaa aagtgccgat attgtctgaa tatgaagaag cgattggggc
    39121 taagattaaa gaagcctact tagcacgtga tttatttttc gtattagaag atgaaaaaac
    39181 ggtggctaaa ttgcagccag attttacagc tatcaaaaat tttgaattag gaataggagt
    39241 catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct agaaccttct ttccaaaatt
    39301 aacaatcaat gaagatccag tatgtggatc agcacattct aatcttattc cttattgggc
    39361 agaaagaatg aataaaacaa aattgtcagc ttatcaaatg tcatcaaggg gcggggatct
    39421 agaatgtgaa ttaaaagaag atcgtgtgat aataggagga aaagcagttt tgttttctaa
    39481 aggtacctcg tttatttgat ttctttcaaa tgaattaaaa ataaattggt ggagagtggc
    39541 aaccttatag ctacaatggt tgttaaaaaa agacgcaaac tttttcaatt taatgaaaag
    39601 tttgcgtctc attttaatta ttggtgtttg ttttttattc aatcaagagt aaaatgcccc
    39661 atactaacta tcctcggatg acttcgattt tataaccatc agggtcaatg acaaagtagt
    39721 aagaaggcgc agtgccaggt aatcctttta agtcagtaat ctcaagaccg gcagctttat
    39781 gttgctcgtg taatttttct agatcatcag ctgctatagc gatatgacca tagccgtctc
    39841 ctagatcata cgcaccatga tcatagttgt aagtcaactc taattcatag tcatccccag
    39901 gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa atcacggcgg cggctttctt
    39961 caaaaccaaa tgccttttga taaaattcga ttgatgcttc aagatctttc actcgaacac
    40021 atgtgtgagc cattttcata gattcatctc tccttttcct tttactcaat cataacaaaa
    40081 agaagtaagg aaatagttga tttgtgcttg ttcatgagaa atttctcatg taaaatggat
    40141 agaaaataaa actgttaagg tggaaactat tcatggataa accaattttt ggtgaaaaaa
    40201 agcaatttgc tgattatcaa acccgttatg cagcatatat tgtcatagaa agagatcagc
    40261 agattgcagt aatcgaggct ccaaatggtg ctttttttct tcctggcggg gaaattgaag
    40321 gagaagagac gaaagaagaa gcgattgtcc gtgagctgat agaagaaatg ggaattgtgg
    40381 cagaaatttc ttcttatctg ggagaagctg atgagtattt ttactcgaat tatcgtgcaa
    40441 cttattatta taatccagga tatttctttg ttgctgattc ttggaagcgt ataggagaac
    40501 caactgaaaa gaccaataaa atttggtggg tgagccctca agaagcgctt gtgaaattga
    40561 aacgtggtag ccaccgatgg gcagtcgaga gatggatcga aaaaaataaa aactgaaaga
    40621 aagcgagtag atcagctttc tctcagtttt tttatttatt acagtacata ttttttgatt
    40681 gcttcagcta caccgtgctc gtcgtttgat gaagtgatga catttgctgc ttctttgaca
    40741 agagaaacgg catttcccat tgctactcct agaccagcat attcaatcat ggataagtcg
    40801 ttctcgttat ctccgatagc catgatttca tcttgatcaa taccaaggtg ctgtgcgaga
    40861 ttcgcaactg ccgcaccttt acttgcttct ttatttagta cttcgaagta atagggcgca
    40921 cttttgaccg ttgtgtattt atcacggaaa gtttgcggta aacgcgcaat tgctgcatct
    40981 aaaatttctg gttcgtcgat catcatcatt ttgacgatgt tcatgtctgg tgtcatttct
    41041 tcaggtgttc gatattttag cggcatcttg actaaacttg cttcgtggat cgtataagcg
    41101 ctgatatctc gattaggcgt gtagatcgct ttgtcatcga tcgaatggag atgactgccg
    41161 acacggcgtg ccatcacttc gatctctagg aaatcttcat gtgtcagtcc gtgacgggcg
    41221 atgattttac ctgatttcgt ttgttgtacg agtgccccgt tataagtaat aacgtaatca
    41281 ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga cacctggtaa gggacggcca
    41341 gtgcataaaa cgatcgaaac accttttgca acagcagcgg caatcgcttc ttttacttct
    41401 tttgttactt ctcttttctc atttaataaa gtaccatcga tgtcgatagc gactagtttg
    41461 atagacataa tttcctccaa tatattttaa tttatttcaa tcaatgcgcc attacggata
    41521 tggctggcaa attcttgata agtttcgtca aataaatcat aatgatcacg caaggacgga
    41581 tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt gttcttgtcc cgctaatgca
    41641 gcaacaagct gactaacctg tgataattct atcaaagttc cgtcatttcg caaaatctcg
    41701 atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat cataaggtag atcgtagcta
    41761 gaattcacag cagtgtaata aacaggatta tatccgacct tttctactaa caaagtgagt
    41821 tcttggacta attttgaatc ccggttgcca gaaaaagtag cagatttcag tggtttacga
    41881 ttcaaaaaac gtttagctaa atcatttaaa ataggatcag cagattcaga ccattgagta
    41941 aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt cttctaaagt aaagcttccg
    42001 tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac tgctggcatc ttcttgatac
    42061 aactcgtgtg cgcgatgaag cagatgctcc aaaattactt ccataccacg ggaaacagga
    42121 tggaagtaca cttgcacata catttgatat cggctaacga tataatcttc cactgcatgc
    42181 atgccactca tggaaaaagc gatcccgcct tcataaggac gaatcacacg taatattcgg
    42241 gtaagatcaa atgttccata ttctgttcca gtgaaataag catcccgaag caaatagtcc
    42301 attcgatctg catcgatctg actagaaatc atttggacaa cttgtgggtt ggggtaagtt
    42361 ttttggatca cactagctac cttttcagga aaaccttctt ctactcggtt aagaatctga
    42421 tacacttctg tttctggaga agtgatgatc tctacagtga tggcttcatg attcgtatgg
    42481 aagatatgtt caaatgtatg agagtaagga ccatgaccga catcatgcaa cagtgccgca
    42541 cataaagtca ccagccgttc ctgatcatcc cagcctccgt tgcctatttt ttcttttgaa
    42601 aaatttcgag aaaagatatc gcagattcgt cttgaaatct cataaacgcc taatgaatga
    42661 gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag aagtgcctaa ttgtttaatg
    42721 cgtcgtagac gttggacttc tttcgaattg atcaaatcta agattacttg atgctggaca
    42781 tggatatagt tatggactgg atcacgaaaa actttttcca taggcagcat ttgatattta
    42841 taaggggttg tcataatgta gctcctttat gttattacgt tgttccattc tctggatttg
    42901 gttgttccat tgctccagct ggatttcttt ttttagccag tcggtagaat cctgtaaatc
    42961 acttgcagaa tacagctttt caaaagcttt tgcaaatgct tctttgactt cttttacaaa
    43021 aaggggttgg tcaagcagtt cttcaagtgt tgccattgta gcaggatcca ctgcaggata
    43081 gccatttgta ccaaagtctt cttttaaagc agattgataa aatcgcttca tgacttgccc
    43141 gcgtttttcc tgattaccgt tgacacttaa gtacatcata acagaaatcc catttttgat
    43201 ccgacgctga gcgataccgg caaatttttt gccatggatg cttaaatcat aagttccagg
    43261 acaataagaa tgggtgattt cgaatgcttg tatcggtgcc tttggaaaag cgtatttagt
    43321 caataggagc atttgctcgt aagcgtcatc tatggttatt ttttttgtgt cactttgggg
    43381 gaaaatcaaa gaaatattca aaattccttc atctgatacg atccctaatc cacctgaatt
    43441 tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg atcccgtcac ttagaaaagg
    43501 agttcgaaca tccttcatac ctaagatgac tgtgcagtca tattgccaga aatgtaaaaa
    43561 gatttgctgg tgttctttag aatattccgt taatatatct gttaatgcaa aaggggaaaa
    43621 gtcgtttttt tgataaatgt gctgatctag tcgaatcgca gggttcattc aaccggctcc
    43681 tttgtataaa ttcatatttt ctacaatatt atactctaaa atcaaagagt tgttttggaa
    43741 aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa caaaagaaac taggaacaat
    43801 gtagctatga gaagtaaagg agaatgaata atggaattat cacaaggaac accgatttca
    43861 atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc aagattttta ttttgatttg
    43921 aaagggcaaa tggtgacaat tggcgatacg ctgtacatac gttataaaga gattcaggag
    43981 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
    44041 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
    44101 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
    44161 agtttgaaag accgtcccac aggagggaag gttcgaatcg attatgatct gttcatggga
    44221 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
    44281 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
    44341 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
    44401 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
    44461 atccaaaact atcttgggaa atcagacagt gaaattcgtg attcactcgc acaattttat
    44521 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
    44581 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
    44641 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
    44701 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
    44761 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
    44821 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
    44881 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
    44941 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
    45001 tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat
    45061 aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac
    45121 gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac
    45181 ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa
    45241 tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag
    45301 atagttttca taaagccctg aactattttg aaaaagaaaa agagatcgag cattttagtg
    45361 catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga
    45421 cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg
    45481 ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc
    45541 tctacttttt tgaaggattt cgtttgaaag aaattgcgac tatcttggat atgaatgaaa
    45601 acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa
    45661 ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat
    45721 aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa
    45781 gagcaatcat ttataaaagc aaagcggaga aagagacgtc tccaagggtt cggagtatct
    45841 ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt
    45901 gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact
    45961 gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa
    46021 aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa
    46081 aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat
    46141 cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca
    46201 gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc
    46261 tcacttccac ttttattcaa agatgacacc tatctatctg ttttgacaaa agaagtcaaa
    46321 cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat
    46381 attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg
    46441 aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg
    46501 ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat
    46561 tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac
    46621 aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag
    46681 ttatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct
    46741 tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac
    46801 aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca
    46861 ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca atcaacacaa gacagtacaa
    46921 aaacgagccg agatccggct gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag
    46981 cgaccacaac accgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gccacttttt
    47041 tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag
    47101 ggcacgagat tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt
    47161 cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaagtcact ggcaaaaccc
    47221 ccacttttgt tcgtccgccc tatggttctg tcacgaatgt aggagcaacg atcatccagc
    47281 gaccgatcat cgaatggtct gttgattcag aagattggaa gacaagaaat cctgatttga
    47341 ttttgcagaa gatccaagca acagtatacg atggggcaat tattttattc catgatatct
    47401 atcctgaaac gattcgtgcc gttcctcaag tgatcgatta tctgaaagag caagggtatc
    47461 gcatcacgac agttggggat ttattagggc atccaacggc tgtcgagaac tattatggca
    47521 gaaacgatca tcggccagtt cagtaagtga gacaagtatc tttaataaat aacacatttt
    47581 aataagtagt tgaacacata actaccttac ttgcaaaggc ttaacaatat ctatacaggt
    47641 acaacaaaaa actgtccacc aattttcttt ggcggacagt tttcacttaa aaataaagaa
    47701 gcaactattc gtaaattatg ctaaaatagc agtagaacgc gtcctgaggg aatcgaaccc
    47761 ccgtctcaag aaccggaatc ttacgtgata tccactacac taaggacgca agcactggga
    47821 taattgtaac taaacagata aaaaattaca aggggattgg acaaaaaaat gaaatttgag
    47881 caaaattttt cacaaaaaca gcagcagaca cagaaactag ccatgacgca acagttgcaa
    47941 cagtccatcc aagttttaca atataacagt gaggagctgt tggcattcgt tgagaatcaa
    48001 gcaatggaaa atccattggt ggaagttgtt gagccggaat ggcagccaga ttacataaaa
    48061 gcttcttcat cttcttatga aggagaagag acaaattacc taaatcagat tcctgataca
    48121 aaagggtctt tatttgattc attgatcgaa caagtccatt tgaattaccg agatactttt
    48181 ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt tgaacggatt tttgacgatt
    48241 agtttagaag aagccggaaa agagattgga gctacgccta tccaaatgct cgatgcgttg
    48301 actctgattc agcaattaga accagctgga gtaggtgcac gtagtttgca agaatgtctc
    48361 atgcttcaaa cagaacgaga tgattatgcg ccggaattag cttatatcgt gttggaagaa
    48421 tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg cacaacgttt ttctgttgat
    48481 ctacatgctg ttcagcagat attcgactat ttacaaacgt tgagtccaag ccctggccgg
    48541 attttcgacc gtagcagcga attgtacatt attcctgatg tccgggtatt agtggacgac
    48601 gataaaaatg tacaagttat ctcaaaccga aaaaatcagc caaatatacg ctttcaagag
    48661 agttacttca aacaaatgtc acaacaagca gataaagaaa ctgaaaatta tttaaaagaa
    48721 cggaaacagg aattcgaatg gttgaagaaa acgatcctac aacgtgggga tacaattttg
    48781 cgggttgctc aagtcattgt ttcccggcaa aaagctttct ttatcgacaa ggaacgtcca
    48841 atcaagcctc ttacgttaaa ggaagttgca actgagatag atgtgcatga atccacggtc
    48901 agtcgtgcag tcaacgggaa atatttggaa acagacttcg gtgtatttga attaaaaaaa
    48961 tttttcacta cacgaattcc tacgaatagt acagaacaga cagaagacct ttcagcagat
    49021 acagcgaaga aaaagctgca ggaattagtc gatcaagaag acaaaaacaa gccgttgtct
    49081 gaccagaaat tggttgaatt gctgaaaaaa gatgagatag cgatctcaag acgaacagta
    49141 gcaaaatatc gtgatttatt aggaatcccg agttcatcaa aaagaaaacg ttatgataac
    49201 taatcagtca aaagactaga aaaagtattt cactttttct agtcttttta gttgaaaaac
    49261 gagagcaaaa ctgttagact tacagtcgtg aggttgagat ttccctttat tgatgtgaaa
    49321 ttcgaatagt taatttcttt ttttatcgta tatgggtcgt tttaagtcta cattggacgt
    49381 aaagtgtcca actaaggaga tcgctatgtt ggatgaaata aaaatgattg aatctgttgc
    49441 accagatatt atcgaagtgt tgcaagaacg ctataaaatt ttacgaaata tctattggat
    49501 gcagccgatt ggacgtcgaa gtttatcgga aagtatgggg atcacagaac gagtattgcg
    49561 aacagaaaca gatcttttga aaaatctgaa tttgatcgac acctcaaaaa gcggtatgac
    49621 gctgacaact aagggcgaag aagtttatca aagccttgaa aacttcatgg atcagctttt
    49681 aggtacgcat caaaccgaac aacagttggc agaatatttt ggtaaccaac gctgtatcgt
    49741 tgtttcagga aacagcgaag agcagaccaa agtggctgat gcattcggcg aagcgctgag
    49801 cgaagcccta gaccgactac ttccagaagg cgagaatatc atcgctgtga tgggaggaac
    49861 tactatggct gtggtagccg aacaattgtc taatctggaa aataagaaac ggcataacct
    49921 gtttgtccct gcaagaggcg ggattggtga agcgataact gtacaggcaa attctgtcag
    49981 tgcaagaatg gctgctaaag caaacggtaa ccaccgtgca ctttatgttc cggaacaatt
    50041 aagtctggca acttacaatt ctttactcaa tgagccatct atccaagaag tcttaaactt
    50101 gattagtgaa gcgaattgtg ttattcatag tattgggcgt gcattacaca tggcagcacg
    50161 tcgtaagatg actgaaaaag aattagtcat gctgaaacaa gcaaatgctg ttgcggaatc
    50221 ttttggctac tttttcaatg aaaaaggtga agtcgtttat aaggtcccta gaatcggcat
    50281 agaattgcaa gatttggaaa aagtcccgat catcatggca atcgcgggcg gaaaatcaaa
    50341 agccaaagca attcgagctt atatgaaaaa tgcaccaaaa caaacgtggc tcatcactga
    50401 tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt taaaataaat atttttttga
    50461 ttttcataag gaggaaatct taaatgacag ttaaagtagg tattaatggt tttggacgta
    50521 ttggacgtct agcattccgt cgtatccaag atgtagatgg aatcgaagta gtagcaatca
    50581 acgacttgac agatgctaaa atgttggcac acttgttaaa atacgacaca actcaaggac
    50641 gtttcaacgg aacagttgaa gttcatgaag gttcattcaa cgtaaacgga aaagaagtta
    50701 aagtattagc taaccgcaac ccagaagaat taccatgggg cgaactaggc gtagatatcg
    50761 ttctagaatg tactggtttc ttcacttcta aatcagcagc tgaaaaacat ttaacagctg
    50821 gtgctaaacg cgttgttatc tctgctcctg gcggaaacga tgttccaaca atcgtttata
    50881 acacaaacca cgaaacatta actggtaaag aaacagttat ctcaggtgct tcatgtacta
    50941 ctaactgttt agctcctatg gctaaaacat tgaacgacaa atttggtgtt gttgaaggat
    51001 taatgacaac tatccacgct tacacaggtg accaaatgac tctagacgga cctcatccta
    51061 aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat cgttcctaac tcaacaggtg
    51121 ctgctaaagc tatcggttta gtaattcctg aattgaacgg taaattagac ggagctgctc
    51181 aacgtgttcc tgtaccaaca ggttcattaa cagaattagt aacagttctt gaaaaagaag
    51241 taactgttga cgaaatcaat gcagcaatga aagaagcttc aaacgaatct tacggataca
    51301 acacagacga aatcgtttct tcagatatcg ttggtatgac ttacggttca ttatttgatg
    51361 ctacacaaac taaagtaatg acagttggcg acaaacaatt agttaaaact gttgcttggt
    51421 acgacaacga aatgtcatac actgcacaat tagtacgtac tttagaatac ttcgctaact
    51481 tataagatag acaaacgtac aagtgtaaat tatagacatg tacatttaaa agcggggaag
    51541 caatcgcgct tcttccgctt tttttgataa taagtttatt caggaggaaa taaaatggct
    51601 aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag ttcttgtccg tgttgacttc
    51661 aacgttcctt tgaaagacgg cgtgatcact gacgacactc gtatcaaagc agcattgcca
    51721 acaatcaact acgttctaga acaaggcgga aaagcaatcc ttttctctca ccttggacgt
    51781 gtaaaaacag aagaagataa agaaggaaaa tctttagctc cagttgcaaa acgtctaggc
    51841 gaattattag gtaaagaagt aacattcgta cctgaaactc gcggagaaca attagaagaa
    51901 gcaatccgca acatgaatga cggcgacgtt gtcgtattcg aaaatacacg atttgaagat
    51961 attgacggta aaaaagaaag caaaaatgat acagaactag gtaaatactg ggcttcatta
    52021 ggcgatgtat ttgttaacga tgctttcggt acagctcacc gtgcacatgc ttctaacgta
    52081 ggtatcgctt caactggtat cccaacagtt gctggattct tgatggaaaa agaaatcaag
    52141 ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga ttgcaatcct tggtggcgca
    52201 aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc ctaaagcaga taaaatcttg
    52261 atcggtggcg gaatgactta cacattctac gaagcaaaag gaatcaagat cggtaactct
    52321 ctagtagaag ctgataaagt ggaactagca aaagaattaa tcgaaaaagc aggcgacaaa
    52381 cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt caaatgatgt tgaaacacaa
    52441 gtaatcgaag gagacattcc tgatggtttg atggctttag acatcggccc agcatcagta
    52501 aaattatttg ctgatacatt acaaggtgca aaaacagttg tttggaacgg acctatgggt
    52561 gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg tttgtgaagc aatcgctaac
    52621 ttagaaggcg caacaacaat catcggtggc ggagactcag ccgcagcagc agaacaatta
    52681 ggatttgctg acaaattcac acacatctca acaggtggcg gcgcaagctt ggaattgctt
    52741 gaaggtaaag aattaccagg actagcagca atcaacgata aataaacgac caagtttgct
    52801 caattctgac gagtaatcga tcaaaaggtg aagggagttt tcatgactcc ctagccattt
    52861 ttcaataaat taaggagtgt gttcctcaat gcgtaaacct attatcgcag gtaactggaa
    52921 aatgaacaaa actgcatcag aagcaaaaga atttgctgaa gcagtaaaaa acaaaatccc
    52981 ttcaaatgac aaagttgatt cagtcatcgg atctcctgca ttattcttac aagaattaat
    53041 gacatcagct gaaggtacag aactaaaaat ctcagcacaa aactgctact gggaaaactc
    53101 aggtgcgttc actggtgaaa cttcaccagc agctcttgct gatctaggtg ttcattatgt
    53161 gatcatcgga cattcagaac gtcgtgaata tttccatgaa acagacgaag acatcaataa
    53221 aaaagcgaaa gctatctttg caaatggtat gacaccaatc ctatgctgtg gcgaatcttt
    53281 agaaacctat gaagcaggca aaactgctga atggatcgaa ggacaaatta cagctggttt
    53341 agctgatctt tcagctgaac aagtgtcaaa catggttatt gcttatgaac caatctgggc
    53401 aatcggtact ggtaaatcag ctgacgcaca aatcgctgac gaaatttgtg gagttgtacg
    53461 tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa gcagttcgta tccaatacgg
    53521 cggttctgta aaaccagaaa atattgctga atacatggca aaagaaaatg ttgacggtgc
    53581 tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg tcattgttag acgctgtaaa
    53641 ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg cactaatgag caaaattaac
    53701 taaaatcgta tatgagggaa atcccttagg aaaaaacaaa ctcaaaggag agacaaaaca
    53761 tgtcaattat tactgatgtt tacgctcgcg agatcttaga ctcacgtggt aacccaacaa
    53821 tcgaagtaga agtatacaca gaaagcggag cttttggccg cggaatggtt ccttcaggtg
    53881 cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg agacaaagct cgttatggcg
    53941 gtaaaggtgt aactaaagct gtcgacaacg taaacaacat cattgctgaa gcaatcattg
    54001 gctacgatgt tcgtgaccaa atggctatcg acaaagctat gatcgcttta gacggaactc
    54061 ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt ttctatcgct gttgcacgtg
    54121 cagctgctga ctacctagaa gtacctttat accactacct aggcggattc aacacaaaag
    54181 tattgccaac accaatgatg aacatcatca acggcggatc acatgctgat aactcaatcg
    54241 acttccaaga attcatgatc atgcctgttg gcgctccaac attcaaagaa gctctacgta
    54301 tgggtgctga agtattccac gcattagctt caatcttgaa agctcgcgga ttagcaactt
    54361 ctgttggtga cgaaggtgga tttgctccaa accttggttc aaacgaagaa ggttttgaag
    54421 taatcatcga agcaatcgaa aaagctggct atgtacctgg taaagacgtt gttcttgcta
    54481 tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg tgtttacgta ctagctgatt
    54541 caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt ctacgaagaa ttagtttcta
    54601 aatatccaat catctcaatc gaagacggat tagacgaaaa cgactgggac ggattcaaga
    54661 aattaactga cgtattaggc gacaaagttc aattagttgg tgacgacttg ttcgtaacaa
    54721 acactcaaaa attatctgaa ggtatcgaaa aaggaattgc taactcaatc cttatcaaag
    54781 tgaaccaaat cggtacatta acagaaactt tcgaagctat cgaaatggct aaagaagctg
    54841 gctacacagc agttgtatct caccgttctg gtgaaacaga agattcaaca atctctgata
    54901 tcgctgttgc aacaaacgct ggccaaatca aaactggttc tctatcacgt actgaccgta
    54961 ttgctaaata caaccaatta ctacgtatcg aagaccaact tggtgaagtt gcagaataca
    55021 aaggtttgaa atctttctac aacttaaaaa acaaataatt cttttctaat gaagatacat
    55081 gatcgactat tgaaagatag tcgagtatga tcacttagtt aagtaatcga actctctact
    55141 tgctgaaata tcagcaggta gagagttttt tttgcaacta gcttctgtat atgatgttta
    55201 caaagaatgg tacaagaaaa gatcatcctt gaaaaaggaa agctaacaat aactagcatc
    55261 atataagagc ttttaaggat agaacagtag aataggaatc tatgagcaag tcctctcgta
    55321 taaataaaat aggagggaag tagcagcata tttttcataa agtggctctc tttcttcatt
    55381 tttactttgg ttgctcctat ttgaaaattc gagcacactc aaatacaata aaaagaaatg
    55441 gcaagggaga ggattagatg aaaaaagaag aaaaaatcgc gattttacaa gagatcatcc
    55501 gaattaaatc agtgaatgga aacgaaggtg aagtagcagc ctatttgaat aagcttttgg
    55561 caaagcatga cataacagga gagattgttt cttacagaga tggtagagat aatttgattg
    55621 ctcgttacca aaagggccaa tcaggaaaag tgttaggcct atctgggcat atggatgtcg
    55681 tggcggctgg tgatgaatcg tcttggacct atgcaccctt tgcagcagaa atccatggga
    55741 accgtttgta cggacgtggt gctacagata tgaagagtgg cttggcagca atggtgattg
    55801 ccatgatcga actgaaagag tcaggaaaac catttaacgg aacagtcaaa ttattagcca
    55861 ctgtcggaga agaagtgggt gaactaggtg gggaacagct cacgaaagca ggctatgtag
    55921 atgacttaga tgccttgatc atcggagagc caactaatta cagtttgatg tatacgcaca
    55981 tgggatcaat caactataca gtcacctcgc acggaaaaga agcgcatagc tctatgccag
    56041 accaaggtta taatgcaatc aatcacctaa atgaattcat tacgaaagca aatgctgaaa
    56101 tgaatcacct cgctgaaaca atcgagaatc ctgtcctagg aaaaacaatc cataatgtta
    56161 cattgatcag tggcgggaat caagtcaaca gcatcccaag ccatgcgcag ctccaaggga
    56221 acattcgatc gattccagaa tacccaaatg ataaaatcat tgcattgctg caatcaatcg
    56281 tcaatgaatt gaatcaagag acagactatc atttggaatt gatgatcgat tacaacaaaa
    56341 tacctgtcaa agctgatcca gattctcctt tgattcattg cattcagcaa caattcagcc
    56401 agccattacc tttagttgga gcggcagcaa caacggatgc agccgaattt acgaaagcta
    56461 accattcctt cgactttgtc gtctttgggc ctggtgtcgt tactttgcct catcaagtag
    56521 atgagtatgt tgaaatcgat aattatttgg atatgattga aaaatatcaa ggaattattt
    56581 tatcctactt agcgtaagat ttttttaata aatgaaaaaa cagagccaga ttagcattat
    56641 tttgctaatc cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgt
    56701 ggcaacatag tgatgactaa tgaaacaacg acgatagaca acaaacgatc aaggtagtct
    56761 gtacctgcct gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc
    56821 tgaacgatca tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc
    56881 gaagaaacga tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg
    56941 tttttatctt tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt
    57001 tggattggcg aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg
    57061 gacaatcctg tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg
    57121 atagaatcga gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg
    57181 atcccaatac acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt
    57241 ttgttagccc atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa
    57301 aagaacggtt aaaaactttc ggaaaaaaag acgagtatct acttttgtca taattttact
    57361 gttagcaggt ttctgccaaa attcataacg atcaaccaat gtttgtccac gacttattcc
    57421 ttgagtttcc acagcagtga agcttttgaa accttcacag atggccccct cgataaaata
    57481 agcaacagct aacggatcat tgatgacaca tcctaaaatt ctttcttgtt tccagtggaa
    57541 gtcaaaataa aaacgagtga tcgcttttag atactctccc tcttcaggat tcatttgaca
    57601 gcagtattcg aggatagtcg gtgtcaagac gatttctctt gtcacatcta acccaaccat
    57661 ttctatcgtt tgtttgagat tctcgaatac ataagaagct gcttcaggat cacaccagta
    57721 attgtattca gcaactggcg aacagttacc atggcttttg taagttccac ccatcgagac
    57781 aaaacgatcc atatgtttcc caatatttgg gttgactttc aaagcaaaag cgatattggt
    57841 caaaggaccc aaagcaatga ctgatgtatt tgtcttttct ttgaaataat cagccaaaaa
    57901 atcaacggca tggagaggtt ctgcctgttt tctcaagatc atcgggaaat ttgtttcccc
    57961 taaaccatcc ataccatgag tgtcttgtgc actgatgaaa ggttgcttca aaggtttttc
    58021 cattccttga taaacaggaa tgtctaaacg atcacagcgt tccaggcatt tcagcacatt
    58081 ctgcgtacct atatctgcag ggacattccc acaaactacg gtaatagcaa ctacttcgat
    58141 atcaggggat ttgacagcta atgataaagc taaagtgtcg tcgatccctg gatcgcaatc
    58201 aataattact tttcgcatta tttccaatct cctttcttta gagatactta gttttttatt
    58261 ctgggaagtt tccgaaccag tccaagacga gcgtcttgat tataatgcac gaaattagta
    58321 tactggcttt tattgcgaat gtaaatgctc tcttacaagt aaatctattg aggagaactt
    58381 taagtaaatg atacaatgaa aaagaaatgg aatttacgtg tgccgaaaat aaagaatcct
    58441 atggatcggt acattggata atagttagat tatagtttgg cagaaagcgg cgggtgtaca
    58501 atggttattt tttatttcat tcttattctg ataattatag ggatcagtta ccattttctt
    58561 tcttattttt atgcaaagaa attagtaaga gcgggagtac agagaggata tgactggtat
    58621 gaagacactg gtcatcgttt gatggaccct gctgcagtca cgtcagtaga aaaaaacgac
    58681 aaaaattaga agaagagtgt gaaagctttt gggagcaggg aacacccaaa acaatcaaaa
    58741 gttatgacgg gttgaaacta gctggtcaga tgttcatcca tccaaataag cagaataaat
    58801 gggtcatttg cgtacatgat taccgaagca cagggaaaag agacatgtct catataggta
    58861 aaagatatgc agagaaagga ttcaacgttc ttgtacctga tttacgtgcg cacggagaaa
    58921 gtgaaggaga aatcatcgga atgggatggc tagatcggct agatttaatt gcctggatcc
    58981 aactcatctt agatgaacag ccgaatgcgt ccattatttt acacggcgga tcaatgggag
    59041 cttctacgat catgatggca agtggtgaaa aacttccatc agcggtcaaa ggatttattt
    59101 tagacagcgg atatgtttct gtttatgcag aattccgata tatgttaagt aaaatcactg
    59161 tctttcctaa aaaaatggtc atgagatatg cgaatcatta tgcccagaaa tacgccggtt
    59221 actccttaaa gcaagcatca gctacgagac agcttggcag caaccatctg cctttattgg
    59281 tcattcatgg tgaaagagat cattttgtcc ctacagaagc agcgtacacg attcaaaatg
    59341 ctacggcagg ggataaagca ctgctgcttg ttccagaagc agaacacttg gaagcatcga
    59401 tgaaagatcc gaaaacctat tggacagtga ttttttcatt tattgaacaa agaataacga
    59461 tgaattaatt ttctcttctt ttttgtaagt aattcgagga taaataattg tttatagatc
    59521 aggagactgc taaaataagg taggaataaa aaaatatcag ttatttgagg agtgcggcat
    59581 atggaaatca aagaagaaaa aaatcgtctt gctttattca atgatgaaca acaagagatc
    59641 ggagaaatga catggtcaga tgcgggccca gacatcatga tcattgacca tacatttgta
    59701 gatccagcat atcgtggtca aaagttagct gaaaagctag tgtatactgg agtagaatta
    59761 gcaagacgag acgggaaaaa gatcattcct ttatgccctt atgcaaaaaa agagtttgaa
    59821 aagaaaccag aatatcaaga cgtcttacgt cagtcataag aataacagct gaaagttttg
    59881 aactttcagc tgttattcca tttatataga gttgtcacgg acttttatct atgataaagt
    59941 taaagagacg atatcttttg ggaaggaggc tgcgatatgt ataatttaat tttgggaatc
    60001 gtgatcgttt tatcgattgt tatggtcatt gcgattatga tgcagccaag caagcaaaat
    60061 agtgcagcga gtgcatttac cggcggtgcg gatcaactat tcggcaaaca aaaagctcgc
    60121 ggctttgaag ctgtcatgca gcgatcaact gcagttatgg gagctatctg gatgatctta
    60181 ttatttgtcc tagcatattt atcttcgaag tagttgaata agcctctcgc aaaacggcgg
    60241 gcggcttttt ttattgcaaa gagaacgctt aagaagaagt tatgaaagaa actatggtaa
    60301 aatgaggcga aagaggtgag acgagtgaga caattaccaa aaccccttta tgcaaaacat
    60361 ggaaagcgag cggttttgct gcttcacgct tactcaggaa gtccaaacga tgttcgaatg
    60421 ctcgcacgat ttttggaaaa agcagattat acagtttatg cgccgctttt taaaggacac
    60481 ggaacaatgg agccatatga tattttgcaa gaaaaagcag aaagctggtg ggcagacact
    60541 aaaaaagcca tccatttttt acagtctgag cagttttctg atattgccgt attaggatta
    60601 tcaatgggcg gcatctttgc tgtacgagca ctagaagaag aatcagtgat tggcggcggt
    60661 tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc ctgaaaactt tgaaaaatac
    60721 gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag aaatcaatga aaaagcagtt
    60781 gcttaccgct cgttagctga acaacaatta atggatatac aagatcaggc agcaatcgta
    60841 gaaagtaggt tgtcagatat ccaacagccg atatttttag ctcaagctgg gaaagatgaa
    60901 atgatcgacc ctaatggcgt atttgaaact gcacgcaagt tgagccgtca gcgagtgaca
    60961 cttcgatggt atcctgaaag tggtcatgtg attacagttg gtacagcaag acgcgaatta
    61021 gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga atgaggaata aatatgacaa
    61081 aagaaacgat aaaagagaaa attctctttt tcatggaaag cagtcgcaaa cgcagttttt
    61141 ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag cgatgatttt aaattactag
    61201 tacaaacgat tgctcaaatg gaaagagaac agagtatagt tttcaataaa aaaggaaaag
    61261 tgaaacttcc tttaaaacca atcttgatcg aaggaacatt tcgagcaaat gaacgaggat
    61321 ttggatttgt aacgattgat cccgaagaag atgacatcta tatcccaaaa gaagcgactg
    61381 gttttgcaat ggatggagac accgtagcaa tcgatatcat gaagcgagca aatacagata
    61441 tggatcgcgg agcagagggg aaagtagtcg agattcgaga ccgggcgact actcagttag
    61501 caggggaatt cgttgcttat tcagaagaag aaatacaaga aacggatctt tacggcgttg
    61561 ttatcccgaa agatagaaag ctcaaccaat ttaaagtata tgcagcagca gagggagttc
    61621 gtccagttga cggaagtatt gtgctgatcg aactgaccca ttaccctgaa aaagggtatg
    61681 cgactagttt agaagggata atcaagcaag tgattggtca taagaatgac cctggcatgg
    61741 atatcctctc aattgtcgtg gctaatggta tccctacgaa atttcctgat gatgtattgt
    61801 ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga tataactgga cgtagagatt
    61861 taagaaatca gctcattgtt acgattgatg gagaagatgc aaaagatcta gatgatgcag
    61921 ttacggttaa aaaattagat aacggtaatt attttcttgg agtacacatt gcagatgttt
    61981 cttactatgt gacacaaggc agtcaattgg acagagaagc atatgaaaga gggacaagtg
    62041 tatatttgac tgatcgagtg attccgatga tcccgcaacg tttatcgaat ggtatctgtt
    62101 cactgaatcc ccatgttcca aggctgacca tgagttgcga aatggagatt gatccaaatg
    62161 gacaagtagt ctctcatgat attttcccaa gtgtgatcca aacaacagaa cggatgacct
    62221 acacagctgt caatcaaatc ttagaagatc aagacgaaga aacgatgcaa cgctatgctc
    62281 agctcgtacc tatgttccaa gaaatgggtg aattgcatcg aatccttgaa acgatgcgaa
    62341 tcaaacgtgg cgcgatttct tttgaggatc gcgaagcaaa aatccttgtt gatgctgaag
    62401 gacacccaaa tgatatccag ctaagaagca gaggaattgg tgaacgcttg atcgaatctt
    62461 tcatgcttgc ggccaatgaa acggtagctg aacattataa taaattaaat ctgccgttta
    62521 tttaccgaat ccatgaacag ccaaaagaag aaaaaatgca gcgcttcttt gattttgctt
    62581 cagctttggg cattctggtc cgaggaacga aaggaacgat cacaccaaaa gatttacagc
    62641 atgtgatcga aggagtagaa gacaaaccag aagctgcagt tatcaatact atgttgctaa
    62701 gaagcatgca acaagcacgc tattcagaag acaattttgg gcattatggc ttagcggcag
    62761 attactacac acactttaca tctcctattc gacgctatcc cgatctaatc gtccatcgtc
    62821 tgatccgttc ttacagtgaa gataaaagtg aaagcaatca aagttcgtgg gctacggaac
    62881 ttcctgaaat tgcagatcat agttctaaga tggaacgtcg tgcagtagaa acagaacgtg
    62941 aagtagattc catgaagaaa gcagaattca tgatggacaa agtgggggaa gaattcgatg
    63001 ggatcatcag ctctgttgtg aaattcggcc tgtttatcga actgccgaat acagtagagg
    63061 gattgatcca tatcaatgaa ttgaaacagg actacttcca ttttgttgaa aatcatttag
    63121 ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg acagaaagtg cgagtaaaag
    63181 tagtcaaagc tgatccagat gaacgagcag tagattttga acttgtttcc gcagaagagg
    63241 ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc aggaaaacgc gaaggaaaac
    63301 aagcaggaaa acgcccagaa agaaatggca agaagaacaa acgatatagc gatagtaagc
    63361 aaacgttcgg taagaaaaaa gggaagaagc cattttacaa aggtgttaaa aagaagaaaa
    63421 agaaaaaata aaatgagttg gaggaaacag ctatgccaaa aggcgaggga aaattaattg
    63481 cacaaaacaa gaaagctcgc catgattatt cgatcatcga cacgatggaa gcagggatgg
    63541 tcttgcaagg aaccgagatc aagtcgatac gaaacagccg gatcaatcta aaagatggat
    63601 ttattcgcgt ccgcaacgga gaagctttct tgcataatgt tcatatcagt ccttatgaac
    63661 aaggaaatat ttttaatcat gatccgttgc gcacgagaaa gttattatta cacaaaaaac
    63721 aaatcatccg gcttgaaaat gaattgaaaa atactggaat cactgttgtt cctttaaaag
    63781 tctatattcg taacggctat gccaaggtat tgattggtct ggcgaaaggg aaaaaatctt
    63841 atgataaacg ggaagatttg aaacgaaaag atatcgatcg acaaattgat cgaacattaa
    63901 aaaatttctc tagataattt cattatcctt tttcgcatat tcggtgcttt tgcttttact
    63961 taatgataaa atttgttagc atttaatctg ataaaacatg attatcatag gttttttctt
    64021 agatcaggag caccgtgtat aaagaaaaat tcgcttcgtt tttgactgaa ataaaaaagt
    64081 gtctaatttt catgaaaatg ttttgaattt tctgtttcat ggtggtatga taccgtgtgg
    64141 ttagaaaccg attggaaaaa accagatacg cacatgaatt gattttatgt gaatttcggg
    64201 gaaaagaggt gaaatggatt ggatgaacgc tcgctaacgt tccacattgg accaatttgg
    64261 tttgatggta cagtttgtat aatggtgtta ctgacctgtt tgattgtttt cttcttagct
    64321 tactttttta caagaaatct taagttgaaa ccaacaggca aacaaaatgc gatagaatgg
    64381 gtcattgatt ttagtcgagg aatcgtaaca gataatctgc ctagaaaaga attgagcaat
    64441 ttccatttgt tggcatttac attgttttta tttgtttttg ttgcaaacaa catcggttta
    64501 gtaacaaaaa tcgttttacc taacgaaaca acactttgga aaagcccgac agcagacccg
    64561 tttgttactt taactttggc atttataatg atcttactta ctcacttatt tggggtgaaa
    64621 aagttaggct tcaaaggata tttcgtaaat tcgttcttga aaccatatag ttttatgttt
    64681 ccgatgaaac tgattgaaga atttacaaac ttattaacac ttgctttacg tctttatggg
    64741 aatatttatg cgggggaagt tttgctaacc ttgattgcaa aaatggtaat aagcttaggc
    64801 tggttctcat tgccgttagc aattccatta gagatggtct ggatcgcatt ctccttgttt
    64861 atcggaagca tccaagcatt tgtcttcgtt actttatcta tggtttatat gagccataag
    64921 attgaagtgg aagaataata ttcgttttaa aaacaaacta ctatttatct aatttaggag
    64981 gaaaacaatt atgaattata tcgcagcagc aatcgcaatc atgggagcag caatcggagc
    65041 cggttatggt aacggtcaag ttatctctaa aacaatcgaa tcaatggctc gccaacctga
    65101 aatgtctggt caattccgta caacaatgtt tatcggggta gctctagtcg aagctgttcc
    65161 tatcttaggg gtagttgtcg ccttgattct agtttttggt gtttaattga atcagtatta
    65221 aggcagaaac aatttggttt gttactgctt ttttacatga aaactagaag tacttcaaag
    65281 aaaggaaggc tcgcctatgc tgaatcaatt ggcaattgca gaagttggca atgcgatgtt
    65341 aggaaatgtc atcgttgtca gcggctcatt tttgatacta ctggctctct tgaagcattt
    65401 tgcttgggga ccaatcagcg atattttgaa aaaacgtgaa gacaagatcg ccaatgattt
    65461 ggattctgca gaacaatctc gcatcaactc agcgaaaatg gaacaagaac gcgaacaaca
    65521 attgttagcc tctcgttctg atgcagctga tatcatcaaa aatgcgaaag aaagtggaga
    65581 attaagccgc caaaatattt tgaaggatgc tcaagaagaa gcagctcgtc taaaaagtaa
    65641 agcccaagct gatatcactg tagaacgtga ttcagcgctg aactctgtaa aagacgatgt
    65701 tgcagaacta tctcttcaaa tcgcggaaaa aatcttgaac aaagaattat ctccagaaat
    65761 gcacgaatca ttaatcaatc aatatatcga aggtctaggt tcttcaaatg aaactagata
    65821 aatatacagt aggaagacgt tacgggaaag ctttatttga actggcgatc gattcaaata
    65881 aagcagaaga aatttatcaa gatctcgtaa gcttacgtca aatctatgaa caagtacctg
    65941 gattaggtaa tatgttaagt gatgttcgtc tagaacctca tgaaaaacga gaaatcatgg
    66001 acaagcttgt tagtggctat gatggaatcg ttaaaaactt tttagaagtt gtttatgcgt
    66061 ataaccgaat ggatgatctt ttgttcatga tcgaagaata tgaacgtcga tatgatgaac
    66121 atcaaggttt gctgttggga agtgtcacaa cagcagttcc actttcagac gaacaacgaa
    66181 tacaattaga aaagaatgtt gcaaaaacaa tgaactatca atcagtcgaa ttaaaacaaa
    66241 tcgttgattc ttctattatt ggtggcgcga ttgttgaagc taaccatcga gtaatcgacg
    66301 gaagcattcg cacgcaatta gaaaatatgc gtaatcaatt aaatagatag tacaaagagg
    66361 taggtgaatc gaatggccat caaagcagaa gaaattagtg ccttgattaa agaacaaatt
    66421 gaaaattatc aaaacgtgct ttcagtcgaa gaaatcggta ccgtaactta tgtaggtgac
    66481 gggatcgctc gtgcacatgg tttagaaaac gcgatgagtg gtgagttgct tgaattttca
    66541 aatggctcat acggaatggc gcaaaactta gaatcaaatg atgtaggtat cattatcctt
    66601 ggcgatttcg agtccattcg tgaaggagat aaagtaaaac gaacaggaaa gatcatggaa
    66661 gttccagttg gagaggcctt gattggacgg gtagttaatc cgctaggtca gccaattgac
    66721 ggactaggtg aaatcgttac tgacaaagct cgtccagtag aagcaatggc accaggcgtt
    66781 atgcaacgta aatctgttaa cgaaccaatg caaacaggtc taaaagcaat cgacgctctt
    66841 gtaccaatcg gacgcggcca acgtgaatta gttatcggtg accgtaaaac agggaaaact
    66901 tccattgcga tcgatacgat catcaaccaa aaaggtcaag atatgatctg tatctatgta
    66961 gcaatcggac aaaaagattc tacagttcgt acacaagttg aaacattgaa aaaatatggc
    67021 gcaatggatt acacaatcgt tgtgaatgcc ggtgcgtctc aaccagcgcc gttgctttat
    67081 atcgcaccat atgctggtac tgcaatgggt gaagagttca tgtacaacgg gaaacatgtc
    67141 ttgatcattt ttgatgatct ttcaaaacaa gctgttgctt atcgtgaact ttccttgctg
    67201 cttcgccgtc caccaggtcg tgaagcttat ccaggggatg ttttctactt acactcacgc
    67261 ttattggaac gtgcagcaaa attaagtgat gatttaggtg gcggttcaat gactgcctta
    67321 ccatttgttg aaacacaagc tggagatatc tctgcttata tcccaacaaa cgttatttcg
    67381 atcactgatg gacaaatctt cttagaaagc gacttgttct acgcaggtac tcgtccagcg
    67441 gttgatgccg gactatctgt atctcgtgta gggggttctg cgcaaatcaa agcaatgaaa
    67501 aaagttgctg ggactttacg tctagactta gcaagttacc gtgaattaga agcatttaca
    67561 caattcggtt ctgacttgga tgcagcaaca caagcaaaac taaatcgtgg acgccgtaca
    67621 gtagaaatct tgaaacaaaa attgcatgca ccgctagctg ttgaaaaaca agtagtgatt
    67681 ttgtatgctt tgacacatgg attcttagat agtatccctg tagacagtat tttagacttc
    67741 gaacatgaat tattcgaata tctggatacc aatcattcag atatctttga aacaatccgt
    67801 acaacaaaag atcttccaga agaagaagcg ttgaacagtg cgattcagga atataaagac
    67861 atgttcttag ctacaaaagg aaataattcc tctacagaag ataaattgaa atccattcaa
    67921 aatgcatagt gaggtgagat gaatgggtgc ttcattaaat gaaatcaaga cacgtatcgc
    67981 ttcaacgaaa aaaacgagcc aaatcacgcg ggcaatgcag atggtgtctg cttctaagtt
    68041 gacaaaatct gaagcttctt cacagaagtt tcaggtttat gcaacaaaag taagagaaat
    68101 cgttacgcat ttgactgcta ctcagttaaa tgatattgcc tcagaaaatc ctagagggga
    68161 tatcaattac aacagcatgt tgctcagccg tcctgttaag aaaacgggtt atatcgtcat
    68221 cactgcagat ggcggtttag tcggtggata taacagctcg atattgaaac aaacgatgtc
    68281 tattttagaa gaagaccacc aatcacccga tgattacgtg atgatcgcaa ttggcggaac
    68341 tggtgcagac ttctttaaag cacgcggtat caatctagca tatgagctgc gcaatctatc
    68401 tgatcagcca agctttgatg aagtccgcaa aatcgtaaat atggcaacaa ctatgtacca
    68461 aaacgaagta ttcgatgaat tatacgtatg ttataaccat catatcaatt cgctaactag
    68521 tcagtttcgt gtagaaaaaa tgctgccaat ctcagactta gatccagaag aagcaactac
    68581 tttcgaacaa gaatatattt ttgaaccttc aaaagaagaa atcttgtctc agttattgcc
    68641 tcagtatgct gaaagtttga tctacggtgc gatcgttgac gccaaaacag cagaacatgc
    68701 tgctggtatg acagcaatga aaacagcaac tgataatgct gctaatatca ttgatgagct
    68761 gacggtttct tacaaccgtg caagacaagg ggctattacc caagaaatta ccgaaattgt
    68821 tgccggagct tcggcactag aatagtattg agattggagg aaaaaagatg agttcaggca
    68881 agattgttca agtaatcggt cccgttgtcg acgtggaatt ttctttagac caatccttac
    68941 cagatatcaa caacgcatta gttgtttata aaaatgacga aaataaatca aaagttgttc
    69001 ttgaagcagc gttagaatta ggggacggag tgatccggac cattgcgatg gaatcaacag
    69061 atggtttaca aagaggaatg gaagtcattg atacaggcaa agcaatctct gttcctgtag
    69121 gtaaagaaac attaggtcgc gtattcaacg tactaggaga tacgatcgat ttagaaacac
    69181 ctttcccaga agatgcggaa agaagcgaaa ttcataaaaa agcaccagcc tttgacgaat
    69241 taagtacaag tacagaaatt ttggaaacag ggatcaaagt tatcgatttg cttgccccat
    69301 atttaaaagg tgggaaagtc ggactattcg gtggtgccgg tgttggtaaa accgtactga
    69361 tccaagaact gatccataat atcgcccaag aacatggtgg tatttctgta tttaccggtg
    69421 taggtgaacg tactcgtgaa ggtaatgact tgtattatga aatgaaagat tcaggagtta
    69481 tcgaaaaaac agccatggtg ttcggacaaa tgaacgaacc accaggtgca cgtatgcgtg
    69541 ttgctttgac tggtttgacg attgcggaat atttccgtga tgtagaaggt caagatgtac
    69601 tgttgtttat cgacaacatt ttccgtttca ctcaagctgg atctgaagta tcagccttgt
    69661 taggacggat gccttctgcg gttggttatc aaccaacatt ggcaacagaa atgggtcaat
    69721 tgcaagaacg tatcacatct acgaaaaaag gttcgatcac atcgatccaa gcaatctatg
    69781 tgccagccga tgactacact gacccggcgc cggcaacagc attcgcccat ttggatgcga
    69841 caaccaactt ggaacgtaaa ttaacagaac aagggattta tccagcggta gatccattgg
    69901 cttcttcttc aagtgcttta gcacctgaaa tcgtgggaga agaacattat aaagtagcaa
    69961 cagaagtcca acacgtattg caacgatatc gtgaattaca agatatcatt gctatcttag
    70021 gtatggatga attatccgat caagaaaaag tgttagtttc tcgtgcccgc agagtccaat
    70081 tcttcttgtc acaaaacttt aacgtggctg aacaatttac tggtcttcca ggttcatatg
    70141 tccctgtaga agaaacagtg aaaggattcc gcgaaatctt ggaaggcaaa tacgatgatc
    70201 ttcctgaaga agctttccga agtgttggcc gaatcgaaga cgtcgtagaa aaagcgaaaa
    70261 cactaggcta ctagaaaggg gtgccccatg gatcaatatt taacagtaaa tgtggtaact
    70321 cctgccggtt tggtctatga tcaccatgca gctatcgtcg ttgcacgtac gacagctggt
    70381 gagcttggga ttttgcctaa acatgcacca atcatcgttc cattgacgat tgatgaagtt
    70441 cgtgtaaaac gaacagattc agatacccac gtcgattgga tcgctgttaa tggcggaatc
    70501 atggaagttc gtgataatgt tgtttcaatc gttgctgata gtgcggaacg tgaacgtgat
    70561 atcgatgtat ctcgtgcgga acgtgcaaaa caacgcgcag aacgccaaat cgaagaagca
    70621 aaagcaaaac aagatacgaa cgaattgaaa cgggcaactg ttgcgttgca ccgagcaatc
    70681 aatcgtatca aagtatctaa gcactcataa aatgagatgc ggatgtttat ccgtgtttaa
    70741 gaacagtaaa aagaggttat gacagaagtg gtcagcttca agacataaga aggaatttac
    70801 gaaaattgct tctcaaattt ttgtgaaatc tcagcttatt tttcgaagaa gctacttctg
    70861 gaacaccgta tatcggaggt tgtgaaatca gcgttttgtc ctgagtatta gagaaacaaa
    70921 tggaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagatgattg
    70981 aacaccgttt atttggaata agggactgcg acaagac
//
LOCUS       DACCDF010000005        68907 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCDF010000005 DACCDF010000000
VERSION     DACCDF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.005"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            365..1276
                     /locus_tag="IVK51_000272"
     CDS             365..1276
                     /locus_tag="IVK51_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6429895.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            1493..1930
                     /locus_tag="IVK51_000273"
     CDS             1493..1930
                     /locus_tag="IVK51_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429896.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            2025..2909
                     /locus_tag="IVK51_000274"
     CDS             2025..2909
                     /locus_tag="IVK51_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6429897.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            3133..6300
                     /locus_tag="IVK51_000275"
     CDS             3133..6300
                     /locus_tag="IVK51_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429898.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            6320..6865
                     /locus_tag="IVK51_000276"
     CDS             6320..6865
                     /locus_tag="IVK51_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP6429899.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            6893..7348
                     /locus_tag="IVK51_000277"
     CDS             6893..7348
                     /locus_tag="IVK51_000277"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP6429900.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            7345..8103
                     /locus_tag="IVK51_000278"
     CDS             7345..8103
                     /locus_tag="IVK51_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP6429901.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(8189..8929)
                     /locus_tag="IVK51_000279"
     CDS             complement(8189..8929)
                     /locus_tag="IVK51_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6429902.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            8990..10243
                     /locus_tag="IVK51_000280"
     CDS             8990..10243
                     /locus_tag="IVK51_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6429903.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            10565..10792
                     /locus_tag="IVK51_000281"
     CDS             10565..10792
                     /locus_tag="IVK51_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6429904.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            10915..11241
                     /locus_tag="IVK51_000282"
     CDS             10915..11241
                     /locus_tag="IVK51_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6429905.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            11309..12022
                     /locus_tag="IVK51_000283"
     CDS             11309..12022
                     /locus_tag="IVK51_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6429906.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            12332..13183
                     /locus_tag="IVK51_000284"
     CDS             12332..13183
                     /locus_tag="IVK51_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6429907.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            13304..15169
                     /locus_tag="IVK51_000285"
     CDS             13304..15169
                     /locus_tag="IVK51_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429908.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAEADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHRGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            15217..16626
                     /locus_tag="IVK51_000286"
     CDS             15217..16626
                     /locus_tag="IVK51_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP6429909.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            16734..17435
                     /locus_tag="IVK51_000287"
     CDS             16734..17435
                     /locus_tag="IVK51_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6429910.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            17594..18073
                     /locus_tag="IVK51_000288"
                     /pseudo
     CDS             17594..18073
                     /locus_tag="IVK51_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            18921..19496
                     /locus_tag="IVK51_000289"
     CDS             18921..19496
                     /locus_tag="IVK51_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP6429911.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            19496..19936
                     /locus_tag="IVK51_000290"
     CDS             19496..19936
                     /locus_tag="IVK51_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP6429912.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            19952..20653
                     /locus_tag="IVK51_000291"
     CDS             19952..20653
                     /locus_tag="IVK51_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6429913.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(21141..21566)
                     /locus_tag="IVK51_000292"
     CDS             complement(21141..21566)
                     /locus_tag="IVK51_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429914.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            21940..22911
                     /locus_tag="IVK51_000293"
     CDS             21940..22911
                     /locus_tag="IVK51_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6429915.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            22943..24340
                     /locus_tag="IVK51_000294"
     CDS             22943..24340
                     /locus_tag="IVK51_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6429916.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            24499..25107
                     /locus_tag="IVK51_000295"
     CDS             24499..25107
                     /locus_tag="IVK51_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429917.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQK
                     NESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(25333..25857)
                     /locus_tag="IVK51_000296"
     CDS             complement(25333..25857)
                     /locus_tag="IVK51_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429918.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(25850..26323)
                     /gene="tsaE"
                     /locus_tag="IVK51_000297"
     CDS             complement(25850..26323)
                     /gene="tsaE"
                     /locus_tag="IVK51_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP6429919.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(26446..27426)
                     /gene="pta"
                     /locus_tag="IVK51_000298"
     CDS             complement(26446..27426)
                     /gene="pta"
                     /locus_tag="IVK51_000298"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP6429920.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(27579..28259)
                     /locus_tag="IVK51_000299"
     CDS             complement(27579..28259)
                     /locus_tag="IVK51_000299"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6429921.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            28385..29254
                     /locus_tag="IVK51_000300"
     CDS             28385..29254
                     /locus_tag="IVK51_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6429922.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            29280..29795
                     /locus_tag="IVK51_000301"
     CDS             29280..29795
                     /locus_tag="IVK51_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429923.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(29842..30405)
                     /locus_tag="IVK51_000302"
     CDS             complement(29842..30405)
                     /locus_tag="IVK51_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6429924.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(30460..30639)
                     /locus_tag="IVK51_000303"
     CDS             complement(30460..30639)
                     /locus_tag="IVK51_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429925.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(30716..31225)
                     /locus_tag="IVK51_000304"
     CDS             complement(30716..31225)
                     /locus_tag="IVK51_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429926.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(31321..31701)
                     /locus_tag="IVK51_000305"
     CDS             complement(31321..31701)
                     /locus_tag="IVK51_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6429927.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            32031..32780
                     /locus_tag="IVK51_000306"
     CDS             32031..32780
                     /locus_tag="IVK51_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP6429928.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(32964..34241)
                     /locus_tag="IVK51_000307"
     CDS             complement(32964..34241)
                     /locus_tag="IVK51_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429929.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(34320..34964)
                     /gene="pcp"
                     /locus_tag="IVK51_000308"
     CDS             complement(34320..34964)
                     /gene="pcp"
                     /locus_tag="IVK51_000308"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6429930.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(35163..36107)
                     /locus_tag="IVK51_000309"
     CDS             complement(35163..36107)
                     /locus_tag="IVK51_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6429931.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(36109..36795)
                     /locus_tag="IVK51_000310"
     CDS             complement(36109..36795)
                     /locus_tag="IVK51_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6429932.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            37269..37589
                     /locus_tag="IVK51_000311"
     CDS             37269..37589
                     /locus_tag="IVK51_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6429933.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            37743..38648
                     /locus_tag="IVK51_000312"
     CDS             37743..38648
                     /locus_tag="IVK51_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6429934.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            38645..39418
                     /locus_tag="IVK51_000313"
     CDS             38645..39418
                     /locus_tag="IVK51_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6429935.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(39599..41023)
                     /locus_tag="IVK51_000314"
     CDS             complement(39599..41023)
                     /locus_tag="IVK51_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6429936.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            41234..41680
                     /gene="nrdI"
                     /locus_tag="IVK51_000315"
     CDS             41234..41680
                     /gene="nrdI"
                     /locus_tag="IVK51_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6429937.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(41745..43727)
                     /locus_tag="IVK51_000316"
     CDS             complement(41745..43727)
                     /locus_tag="IVK51_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429938.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(43741..44637)
                     /gene="murQ"
                     /locus_tag="IVK51_000317"
     CDS             complement(43741..44637)
                     /gene="murQ"
                     /locus_tag="IVK51_000317"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6429939.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(44640..45701)
                     /locus_tag="IVK51_000318"
     CDS             complement(44640..45701)
                     /locus_tag="IVK51_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6429940.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(45912..46439)
                     /locus_tag="IVK51_000319"
     CDS             complement(45912..46439)
                     /locus_tag="IVK51_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6429941.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(46506..48008)
                     /locus_tag="IVK51_000320"
     CDS             complement(46506..48008)
                     /locus_tag="IVK51_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6429942.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(48266..49387)
                     /locus_tag="IVK51_000321"
     CDS             complement(48266..49387)
                     /locus_tag="IVK51_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6429943.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            49513..50454
                     /locus_tag="IVK51_000322"
     CDS             49513..50454
                     /locus_tag="IVK51_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6429944.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(50604..51500)
                     /locus_tag="IVK51_000323"
     CDS             complement(50604..51500)
                     /locus_tag="IVK51_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6429945.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            51769..53109
                     /locus_tag="IVK51_000324"
     CDS             51769..53109
                     /locus_tag="IVK51_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6429946.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            53327..53770
                     /locus_tag="IVK51_000325"
     CDS             53327..53770
                     /locus_tag="IVK51_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6429947.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(54274..55692)
                     /gene="nhaC"
                     /locus_tag="IVK51_000326"
     CDS             complement(54274..55692)
                     /gene="nhaC"
                     /locus_tag="IVK51_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP6429948.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(56095..57318)
                     /locus_tag="IVK51_000327"
     CDS             complement(56095..57318)
                     /locus_tag="IVK51_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP6429949.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(57349..58188)
                     /locus_tag="IVK51_000328"
     CDS             complement(57349..58188)
                     /locus_tag="IVK51_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP6429950.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(58205..58885)
                     /locus_tag="IVK51_000329"
     CDS             complement(58205..58885)
                     /locus_tag="IVK51_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429951.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(58887..59960)
                     /locus_tag="IVK51_000330"
     CDS             complement(58887..59960)
                     /locus_tag="IVK51_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6429952.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(60607..61278)
                     /locus_tag="IVK51_000331"
     CDS             complement(60607..61278)
                     /locus_tag="IVK51_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6429953.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(61365..64043)
                     /gene="mgtA"
                     /locus_tag="IVK51_000332"
     CDS             complement(61365..64043)
                     /gene="mgtA"
                     /locus_tag="IVK51_000332"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP6429954.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            64702..65139
                     /gene="ndk"
                     /locus_tag="IVK51_000333"
     CDS             64702..65139
                     /gene="ndk"
                     /locus_tag="IVK51_000333"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP6429955.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(65161..66027)
                     /locus_tag="IVK51_000334"
     CDS             complement(65161..66027)
                     /locus_tag="IVK51_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6429956.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(66187..68280)
                     /locus_tag="IVK51_000335"
     CDS             complement(66187..68280)
                     /locus_tag="IVK51_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6429957.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        21220 a        13302 c        13038 g        21347 t
ORIGIN      
        1 tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt
       61 gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac atttgttcta
      121 agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta acaaaagaag
      181 tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa ctttctggat
      241 tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg ttcttgatga
      301 ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt aggagaattg
      361 ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct tattgtcagg
      421 ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa tcagcgaaca
      481 agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc aacagttgat
      541 cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag aaatcgattc
      601 tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat tgaaatctgc
      661 aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct ttcaagaagg
      721 tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg aatctttcca
      781 tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa
      841 gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt caactgatac
      901 tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa cggttccggc
      961 agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg tgatcacttc
     1021 taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa
     1081 caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa
     1141 attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag acgctaatgt
     1201 aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag attctagtac
     1261 agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc aataaaactg
     1321 ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta cttgatttag
     1381 ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat atttttctgt
     1441 ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat aaatggagtg
     1501 gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg tttttctcac
     1561 aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc atcaattttc
     1621 cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta ttcaaggccg
     1681 tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc ctctgatcgc
     1741 tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc tatttttgtg
     1801 tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat cattttcttt
     1861 tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt tttatcttgt
     1921 caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt ttttctttat
     1981 gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa aaagttttat
     2041 tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa ttagcgatag
     2101 caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa gcaggggatg
     2161 caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt gtcatgggcg
     2221 gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat cggccaaatt
     2281 tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg ataccgctag
     2341 aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg gatattggaa
     2401 aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct gaatcaatca
     2461 atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc atgtcaggaa
     2521 tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat ggaaagaagg
     2581 aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc ggttttgaaa
     2641 cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat ttaaaagatt
     2701 cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta gatgaatcta
     2761 cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct gatgaggaag
     2821 aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca atcaaagtat
     2881 tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga gttggcggga
     2941 gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc cttttttttg
     3001 tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca tattttaaat
     3061 atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa acgtcagaga
     3121 ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt gtttgtcttc
     3181 cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga atcaacgcaa
     3241 tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac ttctgaaact
     3301 acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa gacaagtgag
     3361 acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc aagagctgtt
     3421 tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct tctcgcagac
     3481 gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga attagagatc
     3541 aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac acaccatatt
     3601 tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac agctgcacat
     3661 ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta tatgaccgat
     3721 ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac acttgatttg
     3781 tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac attagatggt
     3841 gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc tccgggtaaa
     3901 gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact ttatggaccg
     3961 tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag tggaaaaaca
     4021 gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga tcgggcagat
     4081 ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat gtttgaagtc
     4141 agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa tgacaacaat
     4201 cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa tggtgggaaa
     4261 atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc tagtcaaatc
     4321 catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga tcaacccggg
     4381 aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg agccggatca
     4441 gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga gaatggtacg
     4501 caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg tgatggcaaa
     4561 tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga cgcttctggt
     4621 tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc aaatatcgat
     4681 atacgcgcag attccggagc aactgtagat acaactggag atattgattt aaattttacg
     4741 gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa gaccggcatc
     4801 atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga tttccaaaac
     4861 aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg aaatggagat
     4921 tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg cgcaacattc
     4981 gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc gatcaatggg
     5041 accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga atttgctcag
     5101 acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct cccgatgatt
     5161 gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt cgttgttccc
     5221 gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt gactttacgt
     5281 attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac gcaaggacaa
     5341 acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca aggcggcttg
     5401 ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt gcaagttgtt
     5461 gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc caaaacgaca
     5521 gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa tgcagcttct
     5581 cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa aaatcaagct
     5641 gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat cccagaaaat
     5701 caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg agaagctact
     5761 gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca caatccaagt
     5821 gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa gatcgtagta
     5881 gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat caaagcaact
     5941 ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg gatttatgat
     6001 cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc tttcacaaat
     6061 gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga gtatcaatta
     6121 gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt gcaagcagat
     6181 tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa agaaaatcag
     6241 atagctggta attactcagc agagataaca tggacattag cagatgcgcc gacagaataa
     6301 agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa acaactgctc
     6361 tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc atgcctccga
     6421 attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga acaaaacata
     6481 tataaatgta aggatggagc ctgggaaaac acaggaaatc ccctacacgg tctttaaccg
     6541 aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga attcaaacgg
     6601 tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt atgatatgaa
     6661 aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg tcactgagac
     6721 actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag ggatcacgtt
     6781 tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca atcagttttc
     6841 tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag atttgaaatt
     6901 aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga atcttcaaaa
     6961 tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca aaaaagaaac
     7021 aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca atacaaattt
     7081 tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc aagcggttgt
     7141 gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa taactgatga
     7201 tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag aaacatttga
     7261 ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg gattatttat
     7321 tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac cgttctagct
     7381 ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc agccactaca
     7441 gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt tgttccagtt
     7501 cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc aaatcaggga
     7561 acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac aatcaagtta
     7621 ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac tggagcacaa
     7681 acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc cggttggaaa
     7741 ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa actaactggt
     7801 tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag cagtggaatg
     7861 ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga tcaggcagta
     7921 gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc attaggagag
     7981 aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa ttatgctgta
     8041 gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga agaggaagcg
     8101 taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc cctgaaatct
     8161 gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg aagtcatgga
     8221 aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca ccgttgaaaa
     8281 attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg atacagtaag
     8341 attttaaaag taaaaagtaa gcatactttt ctaactctgt cttcaattgt tttaaagaag
     8401 tattcactaa tttatcttct gtagttgttt tgaccataac ttggtttttc aaacctttga
     8461 tactttcaat atataaaatt tcccgaggat acaggacgag acttccacct ttttgatcaa
     8521 taaagatttt atcgacattt acgatctgct gttctttcca attgatctgt tctagcactt
     8581 gacgaatttg ttcagataaa ttcctgtctt ttctgatata tccaatcgga aggattttcc
     8641 gattgatgat ctctactgca aaattcgtat aagcggtgaa aaaaacaata ttactatgtg
     8701 catttttttt acgtatttgt tcggcgacat caatacctgt gagtgattgc ttcaagtcga
     8761 tatcgataaa gaaaacatcg tgattatcaa tagtgcccaa ctggatcaac tgattgattt
     8821 gagagataga agtgagctgc acgatattct cgatatgatg attgaccaat tctctgatga
     8881 tccattcgct gtgaaactgg ttatcttcta tgataaaata ctttttcata tgcaggctcc
     8941 tattaaaaaa atatgtgtac attttgaaac aaaacaatgg aggaataaga tggtcgcaac
     9001 agtgacactt tatttgacct ttactttgat tttagattta ggtatctatt tattcttttc
     9061 gaaacaactg ctagtaaaaa acagctttgg ttgtatagct ttgatggtgg tgacactttt
     9121 acttcctatc cctaaagtga gctttcctta cctagttggc ttattattga tgtttctttt
     9181 ttattttact acgaaaaacc tgtttatcag ctgtttgatc agtaatcttt tttttgctat
     9241 cactggactc agcttttttc tggtttttga tctgatggat tatttaggaa agagacatct
     9301 tggaacagca agtttttttg cactacttat cgcatttatt ttctatctat tagtaaaaag
     9361 tatagacaaa cagacgagat ttctcaactt gctttttact tatccaactg aacactattt
     9421 tggaggtatt tgtattttat taagctgggg cgtgatttac ttggtattgg gaagtgttca
     9481 cttccgctca tctaattatc tgcttctatc tcttttgtcg atagtcatca gttttttttc
     9541 tttgtttttt tccattatgt tgatcagttt ccaagtaaaa gaaagacaac atcaggaatc
     9601 gcttcaactg tatcaaataa atgaagaata ttaccatcat ctagaggaat tcaaacatga
     9661 ttataaaagt ctactgtttt cattaaaaat tactcttcag gaaaataaaa aagaaacgtt
     9721 ggaattttta gaaatattag aagatcattc tgcagctatt tttgaaaaac cgcaaataca
     9781 acagctaaca aatattgtat ccccagctgt caggggtgtc tttttaaaat ttctagaaaa
     9841 agcagaatcg gagaaaatcc cgatccaagt agttattcct gtcgaagtga aaacaatccc
     9901 gattgatctg gttgttttta ttcgctgttt atctattttt atttctaacg cattcgatca
     9961 tcgtgtagcc gatcagagtc cgattacgat caatctgctg gaagaaaaag agggatcacg
    10021 ttttagtata gcgaacaagg caaatccaac gaatttgacg ctatcggaga tggtcaaaag
    10081 aggaaaaacc tctaaaaaaa atggcgggtt aggtctatac agtgcgaaaa aaatgttgga
    10141 tgtttacgaa aatgcagaac taaaaatcga attttcaaaa cagaatcact atttttttgt
    10201 agaaatgtat ctggcatcta gaaagagcaa atcagcctac tgacttactg tcaaaggata
    10261 agtttgagga aagtctacac ggagatagag acaacaacac agcatattca aaagaaacag
    10321 aagcgtgatg accgttgtca cccttctgtt tcttttgcta tgtagttaaa caaagtagtt
    10381 gacacttccg ctacttagta ttagtacgaa agtatacttc atgatactaa gtagataaga
    10441 agtgaaaagc ggaacccacg atcgtcaaaa tgctaactgc tttgcttaca ccagataaag
    10501 ggaagagcaa tatcgcttat tttaggcgtg gagaagcaag caaatcccta cgatcacaaa
    10561 aaaaatgctg acccagcagt taagagagtt agaacaagat gaaagtatcc atcgaaaagc
    10621 ctataaccaa gtcccgccta aagtagagta ctcgctcacc gaagaaggaa aatcattaag
    10681 agagattcta gtagcgttgt ctgtttggga agaagaacgt atcgaaaaac aaaaagcctc
    10741 aagggaagaa gacacgctac tgcatacaca tggtggctac ctaaattatt gattttctat
    10801 gacatttatt ttaaataaaa gaaaacagga gtttttttaa aagcatcatc tattttttat
    10861 gtaaaaaaat ggtatgatta atcaagtaag aagtgattgg aagaaggcaa gcaaatgaat
    10921 agtatggaaa aagtcaacca gtttagagat gaacgtaatt ggcgacaatt tcataacgaa
    10981 aaagatttgg ctatctctat ttctttggaa gcatctgaat tgttagaact ttttcaatgg
    11041 aaaacgccgg aagaagtaaa agaaacacaa ttagaacgaa tcaaagaaga actggcagat
    11101 gtattgatct attcatacat gatggcagac aaccttcacc tggatctaga tgagatcatt
    11161 gaagaaaaat tgatcaaaaa taatttgaaa tatcctgtag aaaaaagcgc aggtgtacga
    11221 aaaaaataca cagagttgta gaaatgtgtc attcggctgt tggaaagagc aaaagaaaaa
    11281 cagtaaaatg agtctggagg gagagaaaat gaaaaaaaaa ggaaaagcaa aaagatggct
    11341 agtcaatatc ctcttgttcc tcttgttatt agtaggacta gctttgattt tcaatgaaca
    11401 gatcaaggat tattttgtca gagagacagg agataaatat gctatagcga atgtaacaaa
    11461 agaagatctg aaaaagaata acgataaaga tgtcagtttt gactttgatg cggtagaacc
    11521 aatgacgaca gaaggggtca tgaggtcgca aatgagagga acagatctac cagtgattgc
    11581 tagtatcgca gtcccttcag tttcaatcaa tttaccggtt ttcaaaggct tggataatac
    11641 gtcactactt tacggagcag gaacattatc tccagatcaa gaaatgggaa aaggaaatta
    11701 tgccttagca agccatcgtg caacgaaccc tgaattgctg tttacaccgc tagaaaattt
    11761 agagatgggt gccaagatct atttgacaga tttagaaaat gtgtatacat acaaaacatt
    11821 ttttaaagaa aaagttgctc ctacagatac ccaattactg aatgaagtcg agggaaaaga
    11881 aattgtcaca ctaattactt gtggcgacat ggacgccgtc acgcgactag ttgtacaagg
    11941 agaactggaa agtgttacat cgataaaaga tgcgacagat gatatgagat ctgctttcaa
    12001 tctagagaca aaaacatttt aggatactgg gacataagtc gttacagatt aaaaacagcc
    12061 aaaggatatg aatcgataag gaaaaaccaa tttgctgaaa aaaagagaaa cgcaaagagg
    12121 tgcatctctg atttggttgt tttctagata gacttgtgtt ccagtttttt tattttttat
    12181 atgaaaaggc ttgcataaat aaaaattcga tggtatgatg attttgaata agaaatggat
    12241 tgttactgta cggcaggcaa aaccagaatt cagctaagaa acagtaggcg aattctaggt
    12301 ttttttattt ttcttgagag ggcgaagcaa aatgaaaatt gataaaattt tgaataacaa
    12361 tgtcgtcatt tcgaaaaatg gattcgggga agaagtcgtt tgtatgggaa aaggactagc
    12421 ttttcaaaaa aagattgggg acgagatttc gccagaagcg gtccaaaaag aatttgtttt
    12481 aagagattct tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga
    12541 gatcgaatgg gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc
    12601 gcctaatatt tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga
    12661 aaatatccaa ctgcctaatc cattattatt tgaaacaaaa aaattttatc cgaaagaata
    12721 tcaagcagcg aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg aactttctga
    12781 ggatgaagcc ggttttattg cctttcattt tgtgaatagc caacaaggaa acgaagacat
    12841 gcaagtgacg atgaccgcta ccacaatggt aagagatata ctgagtatca tcagtaaatt
    12901 cttcggcatc gtgttcgatg aagaatcttt gaattatcag cgtatcatta ctcatctgca
    12961 gttttttgcc cagcgttatc tcaaagggga aggatctgac gagcaagatg aatttctcta
    13021 cgcattagtt caaggaaagt atccaaaagc gtttcattgt gtcgaacgga tcaatgagta
    13081 tcttcgacaa acgcaaaaac aggaaatggg gatcgcagag cagatttatt taacgattca
    13141 tattcaaaga gtagtaagtg agaaaaaaag catccaatca taaggattgt tactgtatgg
    13201 caggcaaaac ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc
    13261 tgggttttta ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt
    13321 tggtcgtcgt gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt tagtacattg
    13381 tgctacgcgg ttgcgattca gattaaaaga tgaatcttta gcagatacgc aaaagttaaa
    13441 agaagacccg gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag tagtgattgg
    13501 cagtaatgta gcagatgttt atcaatcgat cgtggatgag gaaggtcttg cagtttcgga
    13561 ggatggaaaa gaagagtcca aaaatatttt gaatcgtctg atcgatatta tctctagtat
    13621 atttacacct tttctagggg cgatggcagc cgccggggta ttgaaaggat tcttatcatt
    13681 ggcaacggtg atgggctggc tgacagctga tagcggtact tatcaaatat tgtttgctga
    13741 agcagatggt gtctttacct tcttacctgt catgcttgct tttacagcag ctaaaaagtt
    13801 caaagccaac cagtttttag cagtagcaat tgctatggca cttgtgtatc cagctatcac
    13861 agcagttgct ggtgcgggag aagctatcag tttctttggg atcccagtga ttctttcacc
    13921 atctggttat acttcttctg tcataccaat cattctagcc gtatgggttc aaagtaaatt
    13981 agagccattc gtaaaaaaag tgattccgca atttctgcaa atgattttag ttccgcttgt
    14041 cgttttagta gtgatggttc cattaacatt tcttgcttta ggaccaatcg ggacggttgc
    14101 cggtaatgct ttaggcggtc tcttcaactc gatttatgga tttagtccaa tcgtagcagg
    14161 tctaatcatg ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatc ggggattcgt
    14221 accgatcatg tttttgaata tcgaacaata tgggtttgat gtattgatgc caatgcttct
    14281 tccagccatc ttggcacaag gtggggctgc tttagctgta gcattgcgaa caaaagatac
    14341 gaaacttcga gcattaggga tttcttctac agtgacttcg ttatttggaa tcactgaacc
    14401 aactgtgtat ggtgtgacat tacctttgaa gaaaccattc attgccgctt gtatttctgg
    14461 aggtattggt ggagctatta tcggattttc cggagtaaaa gctttctcca gcagtttggt
    14521 cagcttattg acgattccga ctttcattaa tacggtggat ggtgtagagt caaatgtgac
    14581 agtagcagta atagctacag gaattgcttt tgttcttgca ttcgtaggta cattgatttt
    14641 aggatttgac gaacaaacac aagacaatca attagaaaac aaacatgcga atgcaggaga
    14701 accgattact tcagcacggc ataccttaaa aagtccttta acgggtaaag tactgccatt
    14761 gagtgaagta ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta tcgctattga
    14821 tccagaagta ggagaactag ttgcacctgc agacggagag atcacgacta tttttccaac
    14881 tggacatgct gtagggatca caacgactga cggagcggag attttgattc atatcgggat
    14941 ggatacagta gaattaaatg ggaatggctt tgagatctta gtcaaacaag gggatctcgt
    15001 caaagcagga gatttattga ttcgttttga catcgaagca attagagcag ctggctatag
    15061 cgtaatcact ccagttgtca ttacgaatac tgatgcattt gccgatatac ttgaattaga
    15121 ccaaaaagag attattgcaa acgaagatgt tctggcaatt gtaaaataag gaggaaatca
    15181 ggatgcaatc acgtttttca gaaaaatttt tatggggtgg agcaactgct gccaaatcaa
    15241 ttagaagggg cttatgatcg agacggaaaa ggactatccg ttgcagatgc tatgcctgga
    15301 ggtaagcagc gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa
    15361 aaatatattt acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt
    15421 gccttgtttg cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt
    15481 tttccaaatg gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg
    15541 attgatgagt gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg
    15601 cctcttcatt tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat
    15661 gaacgatttg ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact
    15721 ttcaatgaaa tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc
    15781 aatggagcag gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt
    15841 agcaaagccg taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt
    15901 atctatgcaa caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc
    15961 caaaaccaag aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca
    16021 tacacagcgc gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa
    16081 aaagacttcc agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg
    16141 tctacggcaa tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga
    16201 ggagtaaaaa atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg
    16261 ctgagaatcg cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa
    16321 aacggtcttg gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg
    16381 atcgattatt tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat
    16441 ttgatgggct atacgccttg gggatgtatc gatcttgtca gtgcatcaac aggagaaatg
    16501 agtaaacgtt acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat
    16561 cgatcaaaga aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac
    16621 ttgtaattaa agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt
    16681 ttttattttt tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa
    16741 ggacacgtgt actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa
    16801 aatatccagt cggttcgatg ttgccgacag aaagtgaatt agaagaactg tttcaagtca
    16861 gcaaaatcac tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga
    16921 aaagtggtcg tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg
    16981 caagctttac gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt
    17041 tgcaattggt ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg
    17101 tttgttttaa gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc
    17161 ttccacaaga aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt
    17221 tgctgacgaa aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt
    17281 taacagaaga agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac
    17341 ggaaatcgtc tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg
    17401 agctgcatcc ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc
    17461 tgccctgcat caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg
    17521 gttaggctta ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg
    17581 gggtgtggca cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc
    17641 gatggtaacc ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa
    17701 tatactcaat cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca
    17761 gatcacagag ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac
    17821 gggagtcggt gggacagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat
    17881 tccggcatcg ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc
    17941 attgttagct ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga
    18001 tccgaatgca gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt
    18061 gacaattagt taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg
    18121 agacaggaat ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt
    18181 aggagcgatc gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa
    18241 atgagaacct ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga aaggaaacaa
    18301 aaaaactttc ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa
    18361 ttattcttca tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg
    18421 caggcgcaac aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata
    18481 tttttacaaa tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca
    18541 aaaatgatca aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt
    18601 atcagtggaa cataacacga gaacaaaatg attttttcgt taataaaaga aacaagtatt
    18661 tgttgtttaa aataatacac gttattggca tataggacga aatcccagta tcttctataa
    18721 gtaagtcatt tcatatcgat gaaaacacgt tatttatttt tgttttatct ataaaatggc
    18781 tgaaaaatga tttttttcta aatactatat atagtggcgt ctatcctata taccactaaa
    18841 aaactacaaa atctagtctt ttattttaaa aaaacacgtg tagaatgaag atagactttt
    18901 gagttaggga gtgttttttt atgaaactta ctgttttttg cggttcccgt tttggtaata
    18961 aagaaagtta caaatttatt gcgcaaacgt taggaaaata catggcacaa gaagaaatcg
    19021 aactggttta cggtggttca tactccggga ttatgggagt gattagtaaa acagtactgg
    19081 aaaataacgg aaaagtcaca ggtatctatc cgaatgggct ttttgaaact gaattgcctg
    19141 gaaaagatgt tactacattt attcctacag aaacgatcga tgaaagaaag gtattattat
    19201 ttgaaaaggg agatgctgtc ctcgttttcc ctggaggtct aggtacattg gaggaattct
    19261 cacaacttct ttcttggata gcaatcggtc tgacgcccga caagccaatc ggaatattga
    19321 atattggcgg ttattacagc ggcttgcaac aattattaga aacttttgca aaagaggatt
    19381 ttatggataa aaaatggttg gatcaagtaa tcttttccaa caatccattg gaactggtca
    19441 atgaattacg agcagtatct attaaaaatc agttattatt gaaggaggca aaataatgag
    19501 tcaaatatat ttagcaggac catttttttc agaggagcaa atcgatcgag taagtcgtat
    19561 cgaaaaagcg ttggaggaaa acaaaacagt cacaagtttc tactctccac gtcatcacca
    19621 agaaagcaat tatgaactat tcagcgctgg atgggcgcag gaagtatacg aaaaagatat
    19681 ggaagaactg acaaatgcag aatttgttgt tgctatcctt gactttgagc atcaaacaat
    19741 cgatccagga acggcttacg aattaggtgt ggcaactatg ttgaaaaaac cgatgatcat
    19801 tgtacaagaa gagacggtac ctacgaactt gatgattaca caaagtctcc atacgtattt
    19861 aaaatcagac caagctgtac gtgaatatga ttttgaaaca ttgcctgttg aaacttacgt
    19921 aggcgagtac ttatagaaga ggaagggttg tatgtcagtg attttgattt caggaacgat
    19981 tggtgcaggt aagagcagtt tgacggatat gcttgcaaaa gagattgatt ctaaaccatt
    20041 ttatgaaaat gtcgaagata acgaagtatt gccgctcttt tattcaaatc ctgagcagta
    20101 cgctttttta ttgcaaatct tctttttgaa caagcgattt ttagcaatga aaaatgcttt
    20161 agtgaacgat gacaatgtgc tagaccgatc catctatgaa gatagcctgt tgttccatct
    20221 aaacgctgat ttaggtcggg taacggatat cgaagtacag cagtacgata gcttattaga
    20281 tacgatgcta aacgagttag atgatgttgc tccaaaaaaa cggcctgatt tgatggtcca
    20341 tatcaaagtt tctttggaca cgatgctaga acgtatcaaa aaacgtgggc gtgactatga
    20401 acagctagaa agcgatgaga cgttatatac ctattatgaa acgttgaaca cccgctataa
    20461 tcaatggtat gaagattttg atgtatgtcc taaaatacaa gtggatgggg ataaattcga
    20521 ttttgtagaa aatccagaag atgcaaaaaa ggtgatccgt cagatccaag aaaaattagc
    20581 tgaattggaa ggaaagcctg ctggaaaata ttttacggat aaccagcatg caaaaggtca
    20641 agaattaggc taaaaataaa aaaccaaacg acgggaaaga aaaattacta cgtgatgtag
    20701 aataagcctt tctatcgttt ggttatttta tattcaaata tttgaaataa ataaatcttt
    20761 actctttgat ttgtaaactg aaatgatcca ctgtttgttt tactgatgaa gcttgatcaa
    20821 aggaagaagc tgagtttgaa gttcataaaa atcagccata gtgagttttg cttggatttg
    20881 tttatcttct tctacttttt acataatttt gaacaggata agattcaaat gttttaaatg
    20941 aatctttgac tagtttgtga cagttgtcgc atatgctcaa gcttccttgg tcattaaatt
    21001 ctgtatggat atttttgttg ttattacatg tacaataatc aggactatcg atcatccatt
    21061 tcgtctcctt tacaatcatc ttgcttcttt actatagcag aaaagaaggg gagaaacaaa
    21121 agaagatcca acagacggca ctatagactg tagatgttgc catcctcatc cttcaaacgg
    21181 taattaccga tttttgtaaa tatgactgtt gtttcttttt tattttcttt tcctgaaata
    21241 atatattcgt catctttggt agtgatcttg atatctgtca gcactgtttt ttcttcagat
    21301 gattcagaag aatcagttcc ttgtgtctga tacgtgcgtg tccattccat ttttttatct
    21361 gtaaatgtca actgtcccgc ggataaatcc atatctttac gtacttttag cgtatataca
    21421 ctgttggtgg tatctgcttt ttccgctttt aagacagaag aatcggttgt ttcgtccgag
    21481 gattggtcag agccgccaaa caaatcacag ccagtcaatg tcaaaagtcc cgcagacaaa
    21541 ataaatagta cgattaattt cttcaaaata cttcccccat ttgataggat aaagaaatta
    21601 taccattgga atgtaaaact tcaaacggat ggaagagaaa aacgcacaaa acacccacaa
    21661 atagagtaaa ttttagaata aaattttaaa aaggcaaaaa atccgccact ttcagaaagc
    21721 gttttaaaaa agtgttgcaa tcttttaaaa aaatgctata ttaaaaatga accggttcaa
    21781 atacttgttt aagtataatt aatagtgaga ggaagttgct ctactgaaga ctttagttat
    21841 atggatattg atgacaagca acactatgca aagaatgcgg tcatcataat cgaacaacaa
    21901 gaaagagaaa tcaaatgtaa ctggcaggag tgagaatgta tggcatcgat ccgtgatgtg
    21961 gctaagatgg ctggtgtttc agtgggaaca gtttcccgtt acttgaatgg tcagcaactg
    22021 aaggaaaaga acatgaaaaa aatagcagaa gcgatttcag cgttggacta taaagaaaac
    22081 atcattgcta aagggttgaa gaataatcga agcttctcta ttggactttt gataaacggg
    22141 atttccagcc gttttgggtc ggaagttgtc tcagggatcg aaagagtagc tgaagaaaat
    22201 gggtatagct tattattaag cgggtttgcc gatcagccag aaaaaattga acaaaaaatt
    22261 gaatacctga tgaagcatgt gatcgatggg ctgattgtct tcttatccga agaagagtgg
    22321 tctggatttg aaatgctcac aaaaatgagt attccagtta tcgcattgaa ttgtccagaa
    22381 ggaccagcag gtattgatac aatattagtg aatgatcgag aaagtgtagc aaaagtgatc
    22441 gctcatcatg gtgaacaagg acataaaaaa atcggattta ttgctgccac tcaaacagac
    22501 tacgtagcaa gagaacgttt agcaggagcc aaagaagcgg tccaaaatga tccatcgatc
    22561 caattagagg tctttacagg agactattct agaaaaagcg gctattacgg ggcaaaagca
    22621 ttattagaaa aaggggcaac tgctattttt gtcagcaatt acaatatgtc gattggtgcc
    22681 atcgaatgtt tcaatcaaga aggtgtcaga atcgggaagg atattgcgtt cagtcactat
    22741 gattatttag ataaaaacga tctgagtatc cttccgaaaa tcacgatcac gccaccaaca
    22801 gataaaatcg gggaacgagc agcagaacag ctgttaaaac gaatcaaaaa agaagaggaa
    22861 gaaaatgaaa aagtgattgt gatggaaaat atcatacatg gaatcaacta aaacgaacta
    22921 aaaaagaaaa agaggtgctt tgatgaaaaa atatcaattt ccagcaaatt tttggtgggg
    22981 ttctgcagca agtggcccac aaacagaagg aaggatttca ggcgatggaa aaggagagaa
    23041 catttttgat tattggtaca aaaaagaacc gcaaaagttc tttgatcaag taggacctga
    23101 aaaaacctcg caagtttata cgaagtatca agaggatgta cagttgatga aacagactgg
    23161 gcataattca tttcgaacat ctatccaatg gagccgtttg atacctgatg aatctggaac
    23221 aatcaacgaa gaagccgttc gtttctatca tcgttatttt gatgagttga ttgaaaacgg
    23281 tatcgaaccc tttgtgaatc tgtatcattt cgatatgccg atgtatctgc aagaaaaagg
    23341 cggatggttg aaccgagaaa cggttgaagc ctatgaaaaa tatgccaaaa gttgttttga
    23401 attgtttggt tcgaaagtaa aaaaatggtt tactcataat gaaccaatcg ttccagtcga
    23461 agcaggttat ttatatcgat ggcattatcc agaagagtca gatatgaaaa aagcgatcca
    23521 agttggctat catgaagcgt tggcttcagc gttagccatc aaatcttacc atgaaatgga
    23581 gcaaggtggt gttattggta tcatcttgaa tctgacacca agctatccaa gagacgaatc
    23641 aaaccctgaa gatgttaaag cagcgcaaat agcagatgcg ttcttcaatc gttcattcct
    23701 tgatccagct gtaaaaggcg agtttccaag tgaattgatt gagatagtaa aagaacttga
    23761 tctcatgcct gtgatccatg cagaggattt gacggttatc aaagaaaata cagtagatct
    23821 tttaggtatc aattattacc agccacgccg ggtaaaagca aaagaaacac caatcgatac
    23881 taaaaacggt ccaatgcctg aggattattt cgacaattat gaaatgcctg gtcgtaaaat
    23941 gaatccttat cgcggatggg agatctatga acaaggaatc tatgatattt tgatcaatgt
    24001 acgagatcat tatggaaata tcgactgctt tatttctgaa aacggtatgg gtgtagaaaa
    24061 cgaagcacga tttatcaaag aagatggcat gatccacgat gattatcgga tcgaatttgt
    24121 ccaatctcat ctgaaatatg tccatcaagc cattcaagaa ggtgccaatt gtttaggcta
    24181 tcatatgtgg acatgtatgg ataactggtc gtggaccaat gcatataaga atcgatatgg
    24241 ctttatttca gtagacttag cacaagatgg aaaaagaaca atcaaaaaat cgggtcattg
    24301 gttcaaagac gtatcagata aaaacggatt tgaggcttaa gaaagaatag ttgagagcaa
    24361 atgcgtgaaa ttctccattc ttaccatttt gtgaaaaaga agttcaatta tttacgatct
    24421 ttttcaactt ttttgtcttt ctatttcatt ttttgtatat aatagaatag tcaaaattaa
    24481 ataggagtgg aggaaagcat gaggaaaaaa agaccgtttc ttctcttttg gttattcggt
    24541 tttttcttac tgcttatttt atccgcagca ttttcaggaa aaatgacagc agatcttgct
    24601 cagaaagaaa cagaaaaaat cgtagcaagc aaagaatcag tagataattt caaaaaagaa
    24661 ttctcaactt attggtgggc ggagtataaa ataactgcca attacgaaaa aggcgaattg
    24721 gacatttatt tgccaaaagg aaaagaagaa aatcaattta ttaatatcga tgacgtactt
    24781 agtgcgattt ctctcagaca atatcaattt aaagacaagc atttgaccgt tttgcttttg
    24841 actcatgatg gcgacaaagt agcccgtttg atcaccgctt attctgaaaa agtagacaaa
    24901 aaagtggtcg ccgatcgttt agcggaatgg aaaaagacaa atgaacaagt caagaaaaca
    24961 actgtctctt ctcaaaaaaa cgagtcgaca ggaacaagtc cttcagattc tggcagtgga
    25021 caaacgataa cgggaagtga agaaaatcaa gcctcaatta ttacgaatga gtcatcacaa
    25081 ggacaatatc aatcctatgg acaatgaaaa acagaggttg tgaaatcagc gttttgacct
    25141 gagtattaga gaaacaaacg gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat
    25201 accgaaggtc agctgattga acaccgttta ttcaaaaaaa atggactgcg acacgacttt
    25261 tgtcacagtc ccttcgtgtg tttttctgcc ttctcaaaag gatgacagtt tctcactctt
    25321 ttcctatttc ttctattcaa tcaaataact cattttatat acatctaaaa attcacctag
    25381 atctgttctt gcccctctag gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag
    25441 gtgtacggct ctttcattac gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat
    25501 ttccttcgcc caactgatga tttcttctag cataagagtg cctagtccca ttccccaata
    25561 ttcctgcaga atactgatac cgacttctcc cacgtgagac aaacgaaatt gactgtctgc
    25621 cttgacagat gctgttccaa taatcgtccc ttcatagatt gcaagcagca ataaattatt
    25681 attgctttcg tatatataat ccaactcttc ttctaaggtt tctggtggta acaggagttc
    25741 tgcttcatcc aaaactaaaa attctgtctc cttattaact tgtcccatca tcaaaagaat
    25801 gtcttttgca tctcctggca ctgcttcccg caagatgatc ccaacttctt cactcatgat
    25861 tttcagccca tttttctaga attcgctgtt tgaaggtttc actttgctcc ccgtatgaga
    25921 ttagtttcac ataccgcttt tctaaatctg tatcactttt ttctaaaatc agttccaaat
    25981 aatcctccgg cagtgcttct tctaatagat ttccccattc aacaacggaa agcccatcac
    26041 cttcaaaata ttcatctaac cctaaatctg ctccgctagc tgctacccga taaatatcca
    26101 tatgatacaa cggaatccga ccttgactat attctctaat aatcgtgtat gtcggacttt
    26161 tgatcatttg cgttatccct aatccttggg caattccttt cgtcaacgtt gttttacccg
    26221 cacctagatc accagtcaat atcagattgt cacctggcat tgcggaatga ccaattgcct
    26281 ctgcgaattg ttctgtcatt gttaatcctg atagttcgat catttttcat cttctcccgc
    26341 tcttctttct ttttgccagt atatcgcgta atgaagtttt tttaaagaaa aaatgaacta
    26401 gtcaagaaca gcagctgctg tttttggcta gttcgcgtgt atttgttaat caatcaatgt
    26461 ttgtgctgct gtgataatcg ataatttata tacatcttct tcatttgctc cacgagacaa
    26521 atcagaaacc ggtttgttca agccttgtag aattggtccg attgcttcaa agttaccgaa
    26581 acgctgagca attttatagc cgatatttcc tgactgcaat tctggaaata caaagactgt
    26641 tgcttgacca gctacttctg aatctggcgc tttcagttgt gcaactgaag gaacataaga
    26701 agcatcaaat tgcaattcgc cgtcgatcat gtattgtggc gctagttttt tagcgatttt
    26761 agttgcttca actactttgt ctacttctgg cgatttcgct gagccttttg tcgagaagct
    26821 catcattgct acttttggtt cgatatcgaa caattctgct gtttttgcag aatctacggc
    26881 gatttcagcc aattcttgcg cagttggatt cacgttgatc gcacaatccg aaaagacata
    26941 tttttcttga tctcttccac gtaccatgat catcgctccg cttgtccgac tgatgccagg
    27001 ctttgttttg atgatctgaa gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc
    27061 agagaccatt ccgtctgcaa tccccatata cgtaagcatc gtaccaaagt aattcacatc
    27121 ttttaaaatc ttttctgctt gttctttgga tgcctttcca tttctacgtt caacaaaagt
    27181 atccaccatt tcatcccact tatcgtaatt atcagggctg atgatcgtaa agctagatgt
    27241 tttgattcct ctggcatgcg ctgcatttct tatttcttct tcgttcccta ctaaaatggg
    27301 ttccacaagt tcttctgctt tcaaccgggc agctgcgcct aaaatacgag catcagtagc
    27361 ttccgggaaa acgattttga tcccacggcg aacaatttta aatcttaaac tatcaaacaa
    27421 ttccacaaga tgacctccag acgtttttat atgtattatc atacactatt ccagaaaaaa
    27481 agaacagttt attttatctg tatcagtcaa aaatgctgga aaaattaagt ttttcacata
    27541 caaagcgaac tgcactataa caggaaagcg ctgttttatt acactgtttc aggcagttgc
    27601 caatcaatcg gttcttctcc taacgcaagg agcgcatcat tcgttttcga gaagggtttg
    27661 gaaccaaaaa agccacgact tgcagataac ggactgggat gtggagatgt tatgatgata
    27721 tggcgactct tatcgatcat tttgattttt tcttgtgccg gttttcccca caagataaac
    27781 acgattggtt tttcccgttc atttaatttt tcaatgatcg tgtctgttaa tctttcccaa
    27841 cctttcccgc gatgagaata cgcttgccct tcccgaacgg taagaacggt gttgagtaaa
    27901 aggactccct gtttagccca tgacactaga tttccatgtt gaacaggtgc gattccaaga
    27961 tcatcataaa gctctttata gatatttctt aacgatggcg ggatctttac gccaggttgc
    28021 acagaaaaag ataagccatg agcttgattt tctccatgat aaggatcttg tcctaaaatc
    28081 accactttaa cttcttcata aggagtcaat tccaatgctt catagatatg atacatatct
    28141 ggatggatct tttgggacgc atattctttt tttaaaaatt ctcttagatg cagataataa
    28201 tcttttgaaa attctgagga caaaacttct tgccaactgt tatgaatgat ttctttcaaa
    28261 aactccacct cctaataaaa agaataactc acattttctt caatcacaag ggacaatctt
    28321 caccctcagc acttgattaa tatggtaaac taggaacaag aagcagaaag gaacgataat
    28381 cattatgatc aaattaattg catcagatat ggacggcaca ttattagatg cccatatgag
    28441 tgtttctcct gaaaatgccg aggcgatacg ttttgcaaat gatgccggtg ttgagttcat
    28501 ggtcgctact ggtcgaaact atcaagaagc tcgcgcagct ttagatgagg taggaatcga
    28561 ttgtgcgatg atcactttga acggggcaca ggtatttgat aaagaaggaa attcattgtt
    28621 taccgtgtcg attccaaata atcaagcaat ctctgtttta gatattctag atgctaacgg
    28681 tatttactat gaagtagcaa ccaacgatgg tctttattct gaaaatcaag cgaaacgaat
    28741 tgaaagtttt gcttccatgg tggctaccca tttacctcac cttacgtata aaatggcgat
    28801 tgccatggcc tcagctaagc tggagttatt gcacatcaca tacgtggaca gtattcgtgc
    28861 tattttggaa gaaaagaatc tggaagtttt aaaaattatt tgttttcata cagaagggcc
    28921 agctgtatta ggtccagttg gaaagcagat ttctctttta ggcgatctag ccgttacttc
    28981 ctctggtcaa aacaatatcg aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc
    29041 ccatgtcgct cacgagcgtg ggatttcttt agaagaagtc atgacgattg gcgataactt
    29101 caatgatgtc agcatgctgc aagtagcagg agtcagtttc gcaatgggaa atgcagaaat
    29161 cgaagtaaaa gattatgcca aatatacaac ggacacaaac cttgaatctg gcgtaggaaa
    29221 agccatattg cgcgcgattg cagaagactt gtagttttat ctagaaaaga gggatataga
    29281 tgtcagaatt tttcattcag gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa
    29341 acgaaaaagg acattccctg tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat
    29401 tgtctcttta tgccatgaat ggcgatctcg ttgctcacat caagcagata agcctgacat
    29461 tcggtacgcg ttttgagctg tataaagagt tcgaaaaggt gggaaccatg cagaaaatat
    29521 tcaattggcc cggtgacttt tattatattc gtagacttca ctggacagtc caaggtgata
    29581 tctataatca tcaatatcaa atccatcatt tcaatcagca aatcatgaaa atggataaag
    29641 caacactttt ttcaggagat tactatgtat tggatgtctc aaatgatgaa gatgctccag
    29701 tttgtatttg tattgctgcg gttttagatt actggctcta ccgtaaagac aaaagtcgcg
    29761 ataagcctgt ttcttaccga cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat
    29821 acgcatttca ggtacatctt tttagatatt cttttgcata tgatcataaa gatgaccact
    29881 gatcatccgc tgaccgacaa ctttgaagtc atggcgttcg tataattttt tagcctttgg
    29941 atttttttca tccacactta gaccgatgac ggagcgatta gctttcttcg ccaatttagg
    30001 taatgcttct aataatcgcg aaccgatgcc ttgtcctcta aaatcttctg atacactgat
    30061 agagtccaaa taccattcgt tgggaaacgt ttctttatca atgaacaaac gtgtagacgc
    30121 atctagctga tatttctcta agataggtac cagtggctga tcgatgattg attcttcttc
    30181 atctgtataa ccgtaagcaa ctcctgcaac tttaccgtct atttcttcta cgatacctcg
    30241 tgaataactg taacgataat ttggatcagt gattgcttct tctaaaatct ctaaagtctt
    30301 ttgttcccct gtctgcttca aaaatggcag ttccatatct tttagaatca ctaagatcaa
    30361 tggggcgatt tctggagcat cttcttttct cgcaaaacga atcatagttt cctctccttt
    30421 tctccattct aacatgcaga aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat
    30481 cttttcagta atgaaataac catgagtaac gattcttgcc gcttcagttc ggcaataagg
    30541 gcagacgact ggcatttctt tatcattatg ttcgactaca ttattgactt cttcgtatgc
    30601 aaattttttt ccgcaattag ggcatttcat tttttccatg ttatctcaac ttcctttcat
    30661 ctttactctc ttagcttact cttatattgt aaaaaagaga agcaatatcc ttcttctatg
    30721 cagatatcca tacctgacgg tcctgttctc catctattaa tcggacttta taataaggac
    30781 gttcatcttt gactagaagt gtccactctt tcacttttgc ttttttcccg tagtagctga
    30841 ctgcttcctt catcgctgct tgcggttgtt tgaactcttc cgttttttct atatcaaaaa
    30901 gatgttctgc ggccttttcc gtgtcatttc gtacggttgt tttcccagtg gatggattga
    30961 ctgctatgac ttgtccttca caaaaaaact ggtattcata tgttgcattc gtaggattct
    31021 ccaaatcttt ttcaactgca aaatgaatag cagatatgtt atgttctttt gtttcgtttt
    31081 ttacgattgc tgctgctttt ttcaatgaga tccgatattc ctggtcacta atatcatcca
    31141 tgtgaaaagc ggcattgagc agtcccgtca taccggtaat ccctattacg atgagggcag
    31201 caacaccaaa catcattttt ttcattcttc attctcctgt ttcttttttt taaagaatat
    31261 caagaaatga acgaatgaaa aatcgaatga aacgaaaaaa aagtgacaat tttgttatct
    31321 ttattcgtgc agttctaatg gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc
    31381 tgtaaaggta tcttctctaa tcggttctga ttcgatccct aaggactgca attctgcaat
    31441 tgtttcttca atatcgtcta ctttgaaagc aagatgacgc aagccacaag cttctggata
    31501 gtttggtcgt tgtggcgcat tagcaattcc aaaaatctcc aattcagcac ttcctagttt
    31561 cagatctaat ttatagtcat ctcgttcttt gcggtaattt tctctgatta cttcaaatcc
    31621 taatttatta acataaaaat cacgtgattt ctgataatct gaaacaatga tggctatatg
    31681 atggatcgtt gtaaggttca tatgagcact cctattcttc gtttaagctc ggatctatat
    31741 taatctccag ttccattgta aatgtgtcag attgaaaaaa atagctgttt ttccttttta
    31801 taagaaaaaa ggcaaaaaaa acagaaagta ggtcctccaa gacactcttc ctgttcccaa
    31861 tataataaat gaaagtaaga aattatattt tcaagaaaac aaacagaaaa ttatcataac
    31921 acaaattatt tataattgaa agactagttt tttttcaaga aaagcataca atagattgtg
    31981 ttagtaaata cagaacacac tacatttagt gtcttaagga gtcctcatac atgaagtcat
    32041 attttcagtt tttagctcat cagctaaaag gctggccgca acaaaattat tatcttttct
    32101 ttttcagtct tggctgccag gtcatgactc ttgtcaatag tcccatcact tggctgtccg
    32161 ttattacatt tatcggtacc acgcttggtg tattgtgtat tttatccatc aatgcggcaa
    32221 aatcagtcaa cggtatttta ggtattttgt ctgcgatttg ttttattatc gttgggtttt
    32281 cagcaaagaa ctatttaagt atcggtgaac agattgctta tatgatcaca ttggatatcc
    32341 ccgtattgtt aagtaaagaa tggaatcata atatggcttc aaaaatccga aaattcactg
    32401 gaaaaagctg gtttatcgca atcatcagta ctttccttgt ctatcttgtt tcaggattcc
    32461 tgattggtcg ttatacagat gatcctcggc cgtgggtaga tgccattgct ttttctatta
    32521 gcttgactgc tggtatcatc agtttcttgc gttacaacaa ccaatattac tggtggttag
    32581 cttcagggct tgctcaaatg gtcctttggt atatttcatt tacgcatgga tctgcaagtt
    32641 tggcgatgtt cgtgaacagc tctgtttact tgctgaatga tgtccttgca tttaccgttt
    32701 caccttggta taaccaaaaa gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca
    32761 acgcacaaca acacgcataa aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc
    32821 tcacgctcta aacacgtata aacggcggaa tcagaagcaa ctccttcgga aataagctga
    32881 aatttacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
    32941 gcttctgtcc cagcctctat tttttatagt aattccttca gatactttct tgcctcatca
    33001 ttgaaacgca gtgattcttc taacttctga taatattcca acgcttgttt ctgagcttca
    33061 gtaaaacgat aatgttttcc tttccatttg tcgattgtct cttgcacatt tccagattgg
    33121 atatctttca ctactagttg ccatttttct tttgagactg tacggtcttt cttatcagca
    33181 gattgtatag cttctcgttc tttacgcaat acttttaaaa aaccatttct gactatccga
    33241 ggaatgctgt cgaagaaccc ttctgtaaaa ccatagcgac aatattgctt ccaaataaat
    33301 agatagattt ttggttggat gaatcgttgg tttttcgcaa aatattccat ctgagcagtg
    33361 acttctggtg ttcgttgacg ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa
    33421 gcattaggat gtactggcac ttcttcatat acgatggatt gccaatcttt acgtaaaaat
    33481 gcgcgatagt tgatcaagaa ttcttctaca tcctttacta attcttctgc aaagtcgtca
    33541 aatagttctt tttttatctg tcgctttttt gactgcccat tgatgtgttc acttattttc
    33601 ttgatttcat tttccaatgc ttgataatcc gctatgtaaa tcggcatttc tacttggcga
    33661 aattctgcga gccgctgagc taattttata tccggtaaat atggctgttt catccgacga
    33721 ttttggtaca agacaaaaca gtatttttgc catagtcctt caggaaaatc gatattcttc
    33781 tgcttaagcc ataataattc gtctaaggcc agatcaactt ttgtcattcc ctgatggatc
    33841 gaagctgcca ctgtcggaga gacatgcagg tgatcggcaa agtggttgat gcctagtttt
    33901 aggatctttc cggtttttgg cgatttaacg atgtattgca cttttaattc tctaccacac
    33961 tcacaataca atttttgctg gcttcgtttt gttcgaaaaa acggattgat cttgaagtca
    34021 gaaaacaccc aatctgttgc agctaaataa tgttcttcca taaaacgaga gctgattaga
    34081 tatcggcggt gcttatcgac tagttcccgc tgttttttcg ttaattttgc atagatctcc
    34141 caacgttgac tgcttgtgta ttgtttagaa attttgatgg gatagctttt gctgatttct
    34201 tttggaaacc attcttcaaa gacggattgc cattcttcca cggctgtttc tttcctttct
    34261 aattacaaaa agctggctac taacataacg ttagcaacca gccagtgttt cttgctaaat
    34321 tagtgagttg ttccggcatt tccggaaata tcttctttat tttctactgc tgcttcaact
    34381 gcataagtca aagaagaagc aatgtcttct aaagacatag aaggcgtacc cactggacga
    34441 tcgatcactt gatcgggtga gaatgacacg tggatgaacc cacctcgaat tgccgggaat
    34501 tgtgtttcga tcaaatgaag caaacgatac atgatatcat tgcagacaaa tgttcctgct
    34561 gtataagaga tgtacgctgg tagaccatga tcttgtacat tttttgtcat tgctttgatt
    34621 ggcaatgagg taaagtaagc ggctggaccg ttttcttcta aagccgtatc gaatggctga
    34681 tttccttcat tatcagggat tcttgcttct gctagattga ttgcgacacg ttcgaacgag
    34741 actgctgacc gcccacctgc ttgtccgaca cagatcacga catctggttg gtgctgttgg
    34801 atggcttctt tgactacttg actgcttttt tcgaatacgg tcggtacttc cactttgatg
    34861 atttcagcac cgctgatttc atctggcatt ttttttactg cttcataggc tggattcact
    34921 ttgtccccac caaatggatc aaaacctgtt actagtactt tcatctgttg ttcctccgtc
    34981 cgattgattt aacatatggt tatcttttat cataccattt tccttgaaca gcgtaaataa
    35041 aggaaacaat agtttaaata acataacaga tcaaacttaa tatattctta taaaaaaata
    35101 gtgttccact acaatgttag aagaggttgc gacaattatt ctgtcacaac ccctctcttc
    35161 tattatttaa atagaatcat ataagcaatt tggaaaacta gcataaataa cgcaataaac
    35221 aactggtttt tgatcactcc gtatttgtct ttcatctcca gcatcgctac aggcacgata
    35281 ttgaagttag cagccatcgg tgtcagcagg gtcccacaaa aaccgcaagt caatgcaacc
    35341 attcctatta acgctggatt cgctccatga ctaaatacga atggtgcacc gatcccaacc
    35401 gtcatcacgg tgatggccgc aaatgcattc cccatgatta ccgtaaatat caccatgcca
    35461 agagcgtaaa tgatgatgcc gatgttcaca tttccttctg gaacgatcgt acctactgca
    35521 ttggaaatca ctgtcccaac tccagcgctt gtaaataccg cacctagaga agctaaaagc
    35581 attggcaaca tgcttaacgg ccctactgtg cccagcatat ctgctgcatc atctaaaaat
    35641 gtacttggtc gattatctcg agaaaagaac atcaagatta gcatggctgc aaaaacaccg
    35701 acgcccacgc cgactaaagc acctaaattg gtaaatagag caaacaagat cgcaaagaca
    35761 ccgatactca aagctggaat aaaaattttc attcctagtt tgtctgacat tttttccata
    35821 tattcttttg atggaagccg gctttctcct ttgctgactt tcttagcaat tgctggtatg
    35881 gtcatcgcaa agatcaagcc accgttcacg attgccggaa gccatcttcc tcctgcaata
    35941 actaccccta agaccgtcca gaaaaaggct gacccataac gatgaggatt ggttttatct
    36001 gttaaatttc ttactccagt gtaaatcaat acgagtccca tcacgatata aaggatctcc
    36061 agtaatttat cgcctaataa aatatcagga tttgtaaaaa agtccatctt attgcccctt
    36121 tcctggataa tatttcttcg ctaatttctt ttctttaaga tagaagtaga cacttgccac
    36181 aactaaagcc gtgatagcca ctggaacttc cactaaagca agctgtccca atgttacgtc
    36241 atatcctaga tctttcagtg ttgattggac tagcaacgcc cctgctcctc cgatgaatag
    36301 tacttggcca aagaaccagc agacattttc catagctgaa gccatacctt ttagttcttc
    36361 ttcatgctta ggatggactt ctaacccttt tgattcgatg gtccccaatg ccatcggtaa
    36421 aatgattgga cgaacaaatc cagcaactcc accaaaactc acgttaaaag ccgcaaagat
    36481 cattcgaaag atcccataaa tatctacaat cataccagca gatactttct tgaatcgctt
    36541 gatcaaggaa gccgctgatt ctttcagacc atttctttca agcgttcctg ttgccaacat
    36601 aatgatgata aaaatcgcca tcccgcgatt gtttacaaaa cttgtaccta aaagttctaa
    36661 cattccttga atgccaagac cgccagtcaa agctgtaaca actaaagcaa ccattacgat
    36721 caatatcgag tctaatttaa atgcaaaccc gagaataaca atcaagacgc ctaaaaggct
    36781 aagataattt tccatatatt ttcctccata aaatgacaat atttttttaa ttataaagca
    36841 aaacacataa aaaatatagt tgttttctca cgaaatagac actattttta aatatttttc
    36901 gttatttttt tatcacaaaa attatcttac ttattgtaag gtaaagcttt ttttaattta
    36961 ttgcccgata atatatagaa ataaggtcta gttactgttt tttttaaatg attaataaat
    37021 aacaacttct ttctatgaaa ttgcaaagca ttcatcttct tttccgctaa cgatatatcc
    37081 gtcagtgctt ggtacacttt actaaaaagt atgagattgc cgatatctgg ataggtgtgc
    37141 tatactacag aaaaagatat acaatctgcc accgggtagg ataaagagga tgatcattcc
    37201 attaggtctt tgaagggatg attttcctct ttttgtgtat ttttttatag ttaaggagga
    37261 atgtaagtat ggcatggatg gaattaatca tagcaggaat tttggaagta ttctggtcaa
    37321 cgatgatgaa atggagtgat ggtttttcta aaatcaatta ttctttttac acagtgattg
    37381 gcatgattgc tagtttttac tttttgtcaa aggcaatcaa aagtttgccg atgagtctct
    37441 cttatcctat ctggacaggg attggcgcag ttggttcggt cataattggt gtggtcttgt
    37501 ttcatgataa gctgaatctt tccacttggt tctttgtggg gatgctactc atcagtatca
    37561 ttggcatcaa gattactagc ggccactaga cacttggaat aagtactgat cagaaaaaat
    37621 aagccttttt atttaatatt caattttttt atgaaaaaat aagaaataag tccaatgacc
    37681 tagcgacttc tccactcatc aggtgtatgc ttgtactatc tatagaaaag gagtgaatat
    37741 ttttgaaaag tctttcggtc caagaattat ccaagaactt tggtcacaaa caagccctca
    37801 gcgatgtcag ttttagctta tcccctggca aaatcgttgg attagtaggt cctaatggcg
    37861 ctggaaaaac aacgatcatg aaagctattt taggactgat ccattactca gatggaacaa
    37921 tctgtattgg tgaaaatgag atttctccct ctagtcataa agggttggaa caagtaggag
    37981 cattgatcga atacccgggt atctatccat tcttaacagg ctatgatcat ctgaagttat
    38041 tctctgaaac caacgatgta tctgctatcg acactatcgt gaatcagctc aagatggaaa
    38101 agtacattca caaaaaagcg aaatcctact cgttgggaat gaaacaaaaa ttgggaattg
    38161 ctcttgcctt actgaaccat ccgtctatcg ttattttaga tgagccgatg aacggtttgg
    38221 atccacaagc aacaagagat gtgcgaaaaa tcattaccga cttggctact cagggcacct
    38281 ctttcttgat ctccagtcac ttactcagcg agcttgaaaa aatggtcgat gagctgatcc
    38341 tgatcaatca aggaaagatc atcaaacaat gtacaatggc tgaattgaac caaacagcgc
    38401 aagatttcat cgttatctct acaacaaatg atctagctgc tcgaacgatc ttgacagaag
    38461 ctggatatac tttggaagat cctgataaaa tcaagctttt aaaagaaaac gaagaattat
    38521 taggaaccgt gatccagcta ttgtctgcac aaaatatcca tgtgacagac gtccagcatc
    38581 tgacgaatga tttggaatct tctctattgg aaattctgga ttcagtaaag ggggaagaag
    38641 aaaaatgaca ttgcttgtaa aacaagaatt atttaaattg atcaagaaaa agtcaactgc
    38701 tgttttatct gttctattag ttgttttgct cataggaaca gcacttctag ccaaaaaata
    38761 cacgacgatc attgatccag ttgaaatgac tgctcagctt tttagtgcca catcttggat
    38821 tgtctttatc atgattgcag ctgcaagcac gatcatttcg atggaagcac aatatggtac
    38881 attgaaaaat ctcctttatc gaaagtattc tcgaggagaa atattagtaa gcaaatggat
    38941 cacattagtt atttattctg tttatttata ccttttagct attattgtca ctgtgttgat
    39001 gaaactgatt cttttccctt cgatttcctt cacggagaag gtttctactg gtcaaacact
    39061 gatccaaagc ttgttcactt atactttggg tagttatatc ggtttatggc tgattttaag
    39121 tttagtgttg atgattgctt gctttatcaa tagttccggc gcatccatct cagcaggaat
    39181 cgtcttttat tttgcttcgt caatcatatc tggtatcctt attgcactca tccaaaaatg
    39241 ggagtggatc aaatggaatc cgatcagtat gttgaatctg caaaaccaaa ttggaaacga
    39301 agagatcatg aagcaattga ctcatctttc tactaatcaa atgctattcg gtaatttagc
    39361 ttacatcgtt ttgttcttag cattaggata tctcgtattt aagcgaaaaa atgtatgatg
    39421 actcatcaaa aaagaggctg tgaaagagac gttcagcctt attgccgatt ggctgtctct
    39481 taaacaccgt ttattctgaa aaaggagctg tgacaacatt tgtgtcacag ctcctttcct
    39541 agtatgtttt tgaataaacg acaggctcac aacctctctt tatacttctt ttatacttct
    39601 atacgtgaag catcccagtg gcaaataaaa ccattgcaat cactccgacc atcgtcacac
    39661 ctataagtaa aatcttatct aagtggatat gttgtacagg ctcgttttgc tccttacgtg
    39721 ctttgaggta aaaataaatg cctggcaaga aaagaacaag aacaagcatt acttcattaa
    39781 aggctacgac tagcatagcc catacttgaa aggcacttgc taaaagccct actaaaaatt
    39841 ggacccattc tttccgctga taagaaagtt tcatttggta caatcctaag aaaagccacg
    39901 tgaagaaaat agctgctgta ccaagtgtgt aagcaaagtt ataagcactg tcagtgaata
    39961 ataatgaaag taaaaagaca ttcgtacata acgtcgtcaa tacgatagca taagttggtg
    40021 ccccattttt gttgagtttt ccccatgctt taggcaaaag tccgtcttca gccatgtttc
    40081 ccattgtttc aatcggtaag atcgtccatg agagccaaac accaactaaa gaaatgatca
    40141 aaccgccgtt gatcacgact gctccccatt taccaaccat catttcaaac acatagccca
    40201 tagcaggctg cgccttgatc gtttgcagct gattcatcgt cattgtccca tatggaagca
    40261 tggaaaccaa gacgtaaata ataatcaagc cgaatatccc tagaatcgtt gctttcccaa
    40321 catctgattg tttttttgct cgtgaaccta cgacagaggc tccttcaata ccaacaaata
    40381 cccaaagcat caccatgatg caactcaata cttgactgga aaacgggatc ggctgtccat
    40441 tcgcattcaa tataatgtcg ctagaaaagt tatcaatgaa ttgttggatc gaaaacgtgc
    40501 tgatagctac tacaataaat aaaaataatg gcactaattt acagaatgtg acgatagcat
    40561 tgacgaaact agcattttcg actccccgat tcactagcca tgcatatccc cataaaaaga
    40621 ctgtactcat cacgatcgat gccagatttt gcccaccttc aaatactggg aagaaatatc
    40681 ctaatgcgct catcaacaat gttgcaaaag ctacgttgcc taaccagcat gtgagccagt
    40741 aaccccaacc gctgataaaa ccagcgaaac ggccaaaacc ttcttctgcg taactaaaga
    40801 tcccgccttt taaatctgga cgttttttat tgatattatt gatcgacaag gagagcatca
    40861 acattgctgt tcctacgagt acccaactga aaatagctgg gccgccagcc gatccggaga
    40921 tgtccgtcat aataccaaat acgccacctc caatcgctga accaattact aaggcagcaa
    40981 gtggaattaa accaattcct tttttctgtt gctctgtatc catgtaagca tcctccaaaa
    41041 taaaaccgct tttatcaaaa agtataacaa acctttttta tttttactta attaataaaa
    41101 actttaatag aaatataatt ctaccggctt ttttctcatt atacaagcaa agcgcctagt
    41161 ccctttccaa attacaccac caaaaaactt aaaaaagatg ctatactaaa aggaaagaat
    41221 tggaggatga tccatgaaca ttttatatat atcgatttca ggaaatacgc gtgcctttgc
    41281 caaacatcta gcagaatatg ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt
    41341 tactttaaaa gaaatccatg aaaatagtga ttttacgaaa gaaacagaac cattttttac
    41401 tttcgttcct acttatttag acggcggaaa tggtttagat aacggtgata cagaaatctt
    41461 gaccgaaacg atgcgtgaat atctggaaca tgaagacaat cataaacttt gtcttggtgt
    41521 agtcggtagc ggaaataaaa acttcaataa tcagtattgt ctaacagcca aacaatatgc
    41581 acaaacattt ggctttcctt tcttggcaga ttatgaatta cggggaacac catctgatgt
    41641 gacacgaatt tatcagattt tagcagaaaa caataaataa taaaaaagaa gaagctgaaa
    41701 aatagatttc agtttcttct ttcgtttcta ttgtttctat catattaagc tttgacatcc
    41761 atcacaggtg cttttgcttt tgctttacct gttttttcca agacaaagtt ttttacacga
    41821 tccatattgg ttaccaagac gatcatggat gtttctttgc ctgctgcttt gatcgcttct
    41881 aagtcagctt ctgctacaag cgtatcaggt gtcacttgct gcccttctgt tactttcaca
    41941 tcaaatggcg taccgcctaa gtcaactgtg ttgattccca tatgaagcaa gatttctaaa
    42001 ccatttggcg ttgtgatccc aatagcgtgt ttcgttggga agacagagct gactacccca
    42061 gtaactggag aatagatatg tccgttttct ggttctacaa agtagccttc acccatcatc
    42121 ttttgtgcaa aaactggatc acttgcttta tctaaagaaa caactgttcc gtcagcaact
    42181 gatgagagga tagtcacact ttcagctgaa gtttgaggta ctactttttc aggcacttca
    42241 tttactgtgg ctgtttcagt cgctaattct tctttttcta aacggtcttt aggaataccg
    42301 aagaagaatg tagcaacgaa gccaccagca taagctgcca ataatccaag gatgtagcct
    42361 aaccatttac cgtcagagat caatgggatc aaagcggctc ctgaaggtcc aatagcgata
    42421 gccccgacat ttccgatcat tccgatcact gccccgccga tacctccacc gatacaagca
    42481 gtaataaatg ggcgtcctag aggtaatgtt actccgtaaa tcaatggttc gccgattcct
    42541 aggatcccga ctggtaaagc tcctttgatc atttctgtta attctttatc ttttttgcag
    42601 cgaatccata atgccaatgc tgcacccact tgtcctgcac cagccatcgc aagaattgga
    42661 agcaatagcg tacgaccagt ttcatcaatc atttgcacat ggattggagt caaaatttga
    42721 tgcagtccga acataaccat cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct
    42781 ccaccaacgc caagtaccca gttgatccca ccgaccatgc catcagaaat gaaacctgct
    42841 aaaggcatga tgaagaaaat ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta
    42901 acgatgatat ctaccgcatc aggtactact ttatgcagtt ttttctcaac gattgacaat
    42961 agccaaacag cgaagatgac acctaggatc ccaccttgtc cagctgcaag agcttcacca
    43021 gtaaataagt ttttcagtgg tgcttccgga ttcatccctg tcagcatcac aacggccccg
    43081 ataactccac caagagttgg tgttgcacca aaaacttgtg cagcattgat acctgtatag
    43141 ataactaggt agctaaacat cccgtttttc aagatgttca tcacatcgat atattgttgc
    43201 catgttgctg catcaagtgt tccggctgtg actaagtttg ctaaaactgc ggcaatccct
    43261 gccacgatcc cagtaccgac aaatgcagga atcattggca caaagatatt agaaatgtct
    43321 tttaataccg atttgatttt agatggtttt tgtttcgatt tttgtgccgc tttcatttct
    43381 tgtgcccgtt cgtttacacg gtcttttcca gatgctgatg aatttgctgt agctgtactt
    43441 acagaagaaa gtttttctcc taatttcacg cctgccaaat caaccatttg ctgtgctact
    43501 ttgttcactt tcccaggtcc gatgatgact tgaagctgtt catcttctac tacaccaagc
    43561 actcctggaa tcgcttttaa ttcagagaca tttacaagat catcattgat gatcttcata
    43621 cgcacacgag tcatgcagtg gacaatgctt tctacattgc ccatgccgcc tgccccttta
    43681 taaatttgtt ttgccagttc ttccaaagtc agttcgtttt ttgccatggt cttttccccc
    43741 tcataaggtc ccttttacaa atccgtttgc ttcagctaat tttgcttgag cttcctcttt
    43801 atcactattt gtcaaaatca tgacgatagc cagcttaaca ttttgatctg cttcttcaaa
    43861 gtacttttca gctgtttcgt aatcagcttc tgtcgcttgc atgatgattc gttttgagcg
    43921 ttcgattagt ttttcatttg ttggtttgac atcgaccatc aagttattga atactttacc
    43981 tataccaatc atactgatag tagaaatcat attcaagata agtttttgtg ctgttcctga
    44041 cttcaatcga gtagagcctg tcaaaaattc tggacctgca tcgacttcga ttgggaaatc
    44101 cgcgtgttta ctgatttcgg cttgtttatt gcaagaaatc gttcctgtga cagcaccgat
    44161 cgattttgcg taatctaatc caccgatcac atatggtgta cgaccgctag ctgcgatacc
    44221 aataactata tcattttttg tcaaattcag cttgattaga tcttcttgcc ctagctcata
    44281 agaatcttcg gccccttcga cagctacagt cattgcttct tctccaccag caattagacc
    44341 tatgaccatt tccggttcta caccgaatgt cggtacgcat tcagcagcat ctaacacacc
    44401 tagtcttcca cttgttccgg ctcccatata aatcagacgt ccaccttttt taaaggactc
    44461 aatcgtgtta gcaatcactg gttcgatact tgtcagttct ttttcaaccg cagcggccac
    44521 tttctgatct tctttgttca ttagttgaac ggcttgagaa atactcatct catctaatcc
    44581 aaaagtttct tggtttctgc gttccgttgt taggttctct aaattgattt tgctcatttt
    44641 tatcttcctt cttttcttat aaattgatag ttcataccag ctttgatttg gttgatcaat
    44701 ggtagctctt ctttgataac tttagctact acattcactt tttcatccgc tggcaaatca
    44761 tatttggtta attggatctc acccatatag cgcaaatact tttcgttatc taaagtaata
    44821 gaccctttca tacgcgtagc tgtatttctt gctgggatag gcggaatctc tcgaaaacgt
    44881 gcatctacgc ttcgtatgac atctcttgca tcgtcttgcc gattcgtatg cttccctaat
    44941 atatattcgt aaaattcttc atctacaggt tccgtatgga gcaagactgt ctgttctttt
    45001 tggaaagaag caaattgctc ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca
    45061 atatccgtgt tacttgctaa tagatctaga gcgcctgcaa gaggatggat ccctcgatgt
    45121 tcttccaaag ttggcaatcc ttgatatagt gggcctctca actctgtatc tcctggtaca
    45181 aaaccttgga caaggagacc ttgaattttg agccattgat tttttttctg ataccattct
    45241 ttgtccagcc ctgtttcggg acgtggataa taattatgcc aagcttcaat ctgtgagaaa
    45301 tcagcttctg cttctttcaa ttcatcgata tctttaggcg tgatcgtgct agcattcaaa
    45361 ctgattttca attgatgtga ccagtcggca atctgctgat tggaaatatg ataatccatt
    45421 cttaaacctg ttacaccaat tgctttcaaa ggcgttagat cttctaatga aaaacccgct
    45481 cgttccaatg cctctcctga aatatcgacc atcaattcta gtttattctt tttcgcttcg
    45541 cttcctagtt cgatcaatcg cttttgatat gccgaaacat catcttctgg tatatgcatg
    45601 gaagtgaaaa taccgacaaa gccattggat gccatttggc ggatatactg tttcttctct
    45661 tctgacaaag aatcgttcat aaatattgaa aagccaaaca tattatccct ctttttctgt
    45721 atccgtcttc atttctatga ctagagtata tatctaaaga aacaaaattt caatactttt
    45781 attaaaataa ttaaatttca tttctaaagg ttgagtttat attatcaaaa gaattgtcat
    45841 aacctaaaaa aggcactgct aagcaaaagc agtgccttga aatattttat ttaaataaca
    45901 caaaaacata attactcata agaaaccaat tctgtattat ctaccatgaa tgtaaagaac
    45961 ccctcattag gcatcggatg aaaataagca ttgatcactc ttatcaaggc attatggcaa
    46021 actaacagat agacattttc ttcgtcttcg atgcattctt tcaataaagg aacgatccgt
    46081 gcatacacat ctaagactga ctccccattt gggaatcgta cagcgaacgc taaacgtgct
    46141 ttttgaaagt tctcatcttt gcttggcagt ccatcataat ccccaaaatc catttctacc
    46201 aatcgttcat caacggtcag cggcaagctg agacgatcag caactgcttg tgctgtatct
    46261 cttgctcgtt gaagagggga atggatgatt ttcgtgattg gaacaggcaa atctactacc
    46321 ttttctgcta gtttctgtgc ctgcatctgt cctttttcag tcagcggcac atctgcccgt
    46381 ccgcaaacta atccttgtgc gttccaagtt gtttctccgt gtctcgttac ataaagcatg
    46441 tgattctctc cttcgttggc aaagttcccc ttaaccatac cttgttgttc ttgagattac
    46501 aagggttaga ttctctcaag agaaataatc tcagtagata ctttatccag gaaggtttga
    46561 tcatgttcca ctagtaacat ggcaggtttc acgttcaaga tcagttgttc taattgttct
    46621 tgattgaaga catccaaata attcaatggt tcatcccatg tatatagttc agctggctgt
    46681 gataaagatt tagccaattc cacttttttc cgttggccca tactcatctg ctcgatcttg
    46741 ttatgaaaaa catctctttc catcccaagc tttcggaggt tgttcaaaaa tgcttggtag
    46801 tctacttggt ttttctctgc aaattccgcc aacgttcctc gattgtcttc ataattttgg
    46861 cttgcataac taatgctcag atgttttggc tggtattttt ctcctataac cttgccgttg
    46921 aatgccccca gaagataatg gataatggat gactttcctg caccgttagg acctgaaatc
    46981 gccacccgct gatgaggctc gattgtaaaa tgaattggct cgaataacag attttcatac
    47041 cctaattgaa gatcttctac gcttaaaagt cgcttatggt gagacgcttg gctattcatc
    47101 gtcaaagaat cgatatattc gatatctttt aatagttttt ctttctcgct gatctgggtc
    47161 tccatccgat tcacgattgc tttggatcgt ttcatcgtcc gtgcagcatc agcacccact
    47221 gctcctttat tcactcgtct agattcagta tcgatgaatc cgacttgttt ctttgtttta
    47281 tctccttctc gggaacgaga ccattcggct ttttcagctg ctgttttctt tagcctactg
    47341 acttcttttt tcaatttttc attttgagcc atttcaaatt catcacgaag ttttttctgt
    47401 tcttcataga tagagaaatt cccttgataa agttccagtt gacttttttc gattgctaaa
    47461 acatggtcca ctacttcatc gataaatccc cggtcatggc tgaccacgat gaagccttgt
    47521 tttttctttt tcaaataagc cgctacttgt tttctaccag agatatccaa atgattcgtt
    47581 ggttcatcga ttaacgggaa atgagtgtca tccacaaata aaagtgccag taggactttt
    47641 gtcttctctc cccccgatag tgtgctgaat tctctccata agatttcagg atctgtttgc
    47701 atcaattgga gctctctttc gatttcccat atctcaaaat ccgtaatatc atttaacacg
    47761 taataggtta aacgttcttt atcttttgtc tgttgcggga aataggcaaa ttcttgctga
    47821 tggattactt gcccttgata aggtagtttg ttttgtagaa tattcagtaa agttgtcttt
    47881 cctcgaccgt ttcgtccgat aagtcctagt ttccattgtg tgtcaaaatt tagatttgct
    47941 tgttcaaata ataatgtgcc ttggctgtcg tagccgaatg tcagattttt tatttcgatt
    48001 ttagacattt tccatcacct cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag
    48061 aggatgcaag ggttggatac acacaaaaag acccatacat cgaaaaaacc aagccaatcc
    48121 cgaaaatctg cacactgctt ccttgtctcc acaaggtaca gtctgaatgc agctttcatc
    48181 aatcgactta atttttcaat gcacggatcc ctcactttgt cctatatttg aaattacgat
    48241 agcataaaga aaatgatttt gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg
    48301 tctgtcctaa gatttttgtt ttttctaatg tttgcctgtg ttccattgct tcttctaaac
    48361 ctattggacc caaatgaata cttaagacaa ttgggagaaa atcagaaagt ccttctagct
    48421 cttggtctct acttccgcaa atagcgatta ttggttttcg atattttttt gcaagcactg
    48481 ctacaccgta tggaactttt ccgtggatcg tttgctgatc gatccgccct tctcctgtaa
    48541 tgatgacatc tgcttgttcg atcttttttt ccaatccagt gatttggctg actaaatcga
    48601 aaccagaaac catcttcgct cccaacaaaa aaagggctcc accaataccg cctgctgctc
    48661 cacttcctgg aatatcatct aaatccacct cccagaaatc ttttatttgc tgagcaatct
    48721 ttcctgcttg tttatctaag aaagctagcg tcgtatctat tcctcctttt tgtcttccaa
    48781 attgccaagc agctccttgt tgacctgtat atggattggt aacatctgca gcgataatca
    48841 attttacttt agaaacaaga acgttttttc gatcaaaatg aagtttttta gtagataata
    48901 aaggatttcc ttctgtgtac ccttctaaag aagcacccaa ggattggagt aatcctagac
    48961 caccatcgct acacccgctt cctcctaaag tgacaataat ttgttctact tcttgaagca
    49021 gtgcatcttt aataagttct cctaaggcat aactggaagc aagtcggata gtttgatccg
    49081 ttggtttatc aagtagatct aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt
    49141 ttccttgcca agttgtcaat aaatagctga tttttcgctg acggaataag agatccatcc
    49201 catcaatggt acgccatgtt cccctgagcg cgtaatagat tgcttctgca gtattttctc
    49261 cgccatctgc aatcggcaca ttgatttttc ttcctttacg atcttcccag ccatttagaa
    49321 ctgcctgatt tagttcaaat gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa
    49381 tatccatctg tatccctcac tttcttctta agtaagacac tttttttcat ttttttcaaa
    49441 caaaacaatg ctattttctt ccatttttct atatactaaa agggaactca ttgaataaag
    49501 gagagaaaaa acatgaaaat cgcaatagct ggggccggag caatgggcag ccgaatcgga
    49561 ttgatgctac atcaaagcgg aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt
    49621 gaagccattc gtacaaatgg gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa
    49681 ctgccaatct attcaccaga agaaatcatc gaaagcaacg aacacgtgga cttgatcgta
    49741 gcattaacaa aagccaatca attagatgat atgttttgtt cgatccaatc cattattact
    49801 gacaatactt atgttttgtg tttactaaat ggattaggtc atgaagatgt actggaaaaa
    49861 tatgtgccaa agaaaaatat cttatttggg atcacgatgt ggacagctgg tctagctggt
    49921 cccggaaaag tcacattgtt aggtgatgga gaaatcgaac tagagaactt ggaaccagaa
    49981 ggcgaagcat ttacaaaaaa agttgtagaa gtctttcaag aagcaaattt aaatccgatc
    50041 tatagccata acgttcgtta ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat
    50101 ggtttatgca cgattttgga ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag
    50161 agcatggttc gaacaatcgt atctgaattt gcttctatcg cagcaaaaga aggaatcata
    50221 ctagatcaag aagaagtgta ccaacatatt gcttctactt atgatcctga caacatcggg
    50281 ttgcattacc catctatgta ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac
    50341 tacatcaatg gtgccatttg gcgaaaagga caaaaatatg atattgctac accatattgt
    50401 gcctttttga cccaattagt ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct
    50461 ctctgatgag aatcagctga atcagcagct ggttctcttt ttttaagccc aataaaagat
    50521 caaaaagaaa acacaactct catgatgcca taagaattgt gcttgtttgt ttcagtacat
    50581 aatctcttca aaatgtaggt tttttatttg ttttcaacag aggagaggtc atattcgtat
    50641 tgctcataaa tagtggaatc gttagtgata aatcgttttg cccaattttc tgcatcataa
    50701 aattccaata gctctgatag gattggctta aatggtgtat gcagaaattc cggtaattca
    50761 tctaaatcac gttctcgaag caaaaaaata ttttcaggac gataaaaatg ataatgagca
    50821 atcgcttgat tatccgtcac tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa
    50881 gtcactcctt gctgaccctc ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt
    50941 ttcaggtact cttcataagt aagatatcca ggttgttcat tccctcgatg tggaagaatg
    51001 aaatagttgg tcgtaacatc tctttttata aattcctgct tatagctttc tgctcgttct
    51061 tttctcccat tattcgttgc accaaaaaat agatccgtct ttggttcgct agaagaaaaa
    51121 ttcatatgtt tcgaataaaa tgaactatta tgtttcagcc caagttcttt tgcttccaaa
    51181 aaatcaaaat gataaatctc atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc
    51241 tcgtgttctg ctttgaagtg attccaaaaa aataacacaa tttttgcatt ggtaaatggg
    51301 cgcacgcgtc gagccatctc catatcaaat ccataatcta aaaaaacgat cagctgataa
    51361 tttgccaact gttgtctagc tgatttaatg aattccgact gtagtaaatc ttttccggac
    51421 caaacatcta aagagcggat ggaaggcaaa aaataaagtg atttattttt tgcaactagc
    51481 agtacgtcat ttttgttcat cttttccgcc tcactttctc tgtcactttt tcctatgata
    51541 gcctgtttaa atagtaaaaa gccagtaaaa cgtttcctat atttgtatga aaatttaaca
    51601 tttgtatgaa aatatgatga aataaatcat tcttctatta aaattcgcct aaaagttttc
    51661 tgaatagttg taattgtctg aaaatttgtt atcattagtc attgttaaat attgctattt
    51721 attcttaata taaattttga ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa
    51781 aaattgaaaa aacaacttag ctccagacat atcaccatgt tagctttagg tggagcaatc
    51841 ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga tcgccggccc ttctgtattg
    51901 attgcttttt tgattggtgg gctgatttta tatatcgtga tgaaaggtct gggaaaaatc
    51961 gttttaagcg gtggcgacac gcatcacgga ttatctggtt tgatacgccc ttatctaggt
    52021 gctcattctg ctgattttac tgattgggtc tactggtcta tgtggattat caatatcatc
    52081 gctgaagctg ttgcagctgc ttcctttttg cagttgtggt tcccgcacat tccagcctgg
    52141 gtatttgttt ttatgttggc tgttttaact acgatcatca atctttattc ggtacggtta
    52201 ttcgcagaaa cagaatattg gctggctttt gctaaaatca gtgtcattat cttactgatc
    52261 atttttgcta cttatcttgt cggtcagcaa atgttgggca caggcgtttt ccctaccctt
    52321 cagcaattga ctgaccatgg tgggtttact cctcatggta tgaagggaat catcagttct
    52381 ttgcttgtag ttatctattc ttacggcggt tctgaactga ttgctatcac ggtaagcgaa
    52441 gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt
    52501 tccttttaca tcattccttt gtttttgctt ttgattcttt ttccttggaa tactttagca
    52561 ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg
    52621 gatatcgtca attttgtgat tgtccttgct ttgttctcat ctatcaattc gggtgtgtat
    52681 gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa aaggttcctc tagaaaacta
    52741 gctgttttaa acaagcatca agtaccgcaa cgtgctgttt tattttgtac cagtgttctt
    52801 tatcttggtg tagctctttc ttattttgtc ggtgacaaat tatttggcta tctggcaggt
    52861 tccctttcgt atacagtatt attgatctgg atcattatca gtgcggcatc ctttgtactg
    52921 gcactaagaa aaggaacatt atgggataaa ggaatcagct tttttgcatt agctgtctta
    52981 ggattgattt tccttggtat cctgttcacg aactccattg gggtaactgt attgactggc
    53041 ctgctttacc tgttcattta tttcagttat caaaaaaaga atgatgcgtt cgtattgacg
    53101 aatgaatgaa atcgatagaa aattttattt tatttatgtg gaatagatgg agctattgaa
    53161 gaaaggagtt gtgacaaaag tcttgttgca actccttttt ccgaataaac agtctcatga
    53221 cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat aattgattgt tctccatgct
    53281 catgacatga tagaagaaag gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg
    53341 gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg tttggtatga tgtacttgaa
    53401 tgtctatgcg ctcgatcata tcttcttcag tcgtacacga gtattcatgg cgttgatgat
    53461 gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa
    53521 gataaatgtt atgatcttag ccgctagtct tcttgttttt gccggttcct tgtttatggt
    53581 acgaagccaa actactgtca acgacacagc ttggatgaag gccatgattc ctcatcattc
    53641 tatcgctatc ctggcaagta aacgagctga tttaaaagat cctgaagtaa aaaaattagc
    53701 ggaagaaatc attcaggcac aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga
    53761 aatggaatga ccattatccg tccaaataaa aaaagaggat gttccagaag ttatcagctt
    53821 caagaaataa gaagaagcta cttctggaac gccgtgaata aggggttgtg aaaaagctgc
    53881 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcctatttt tcgtttggtt
    53941 aggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtaaaatcag
    54001 cgttctgacc tgagtattag agagacaaat ggaaaaatag ttcctcatat tttgccattt
    54061 tttgagctaa taccgagggt cagctgattg aacaccgttt atcgagaggt tgtgaaaaag
    54121 tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg ctttccacat ttttgtataa
    54181 atttggctta ataccgagaa actggctttt gaataccgtt tattcgtaaa agggattgtg
    54241 acaaaagtct tgtcacaatc cctttttatg tttttattgt tctaattgcg ctgtcttgct
    54301 tactgcttct tgcggtttca caaatttttt tgagattccc caacctgtga aggcactgac
    54361 catcgaaaac acaggagaaa gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg
    54421 aataccaaat gtacttgcta cgaaacttcc ggcaattccc caaggcacta agtagttgat
    54481 tactgttcca ccatcttcta gcgttctacc taatacagtt ggatcaaatc ctgctttttc
    54541 ataagtttct ttaaatgcat taccaggcag aataacagat aggaattgtt caccaataaa
    54601 aatatttact ccgatacttg tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat
    54661 ctttcttgat actcgttcca tcacggttga gatcaagcca aattcgatca acaatccacc
    54721 taatgacaaa gttagaataa tcaatgacac agtaggcatc atactttcga ttcctccgcg
    54781 gctaagcagt gaatcgattt gttggtttcc agtatttgaa acaaaccctg attcgatgac
    54841 cccagccaac ttcgttaagg aagtttgtgg gttttgaata aaaatcattc ctactgctac
    54901 agcgatattc aacaaaatcg ttaaaattgc cggcaccttt ttccatgcac acagtaacat
    54961 taaaaataaa ggaatcagtg accagattga aatagtaaag tttgcttcta gaacatcagt
    55021 cacttcacga atagatgaaa gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa
    55081 aaagaaagaa acaacaaatg ccgggacagt tgaccacatc atatgcttga tgtgtttgaa
    55141 caaatcggca tcaacaatag ctgctgctag atttgttgat tcggataatg gagacatctt
    55201 atctccaaaa acggcacctg aaacaatagc tccaacaacc agcgctggat ttaagcctag
    55261 tgttgtccca ataccgaaaa atgcaatccc aatcgttgac ataacagtaa aggcactccc
    55321 tacagcgcta cctacgattg cacacacaac aaatacagaa gggacaaacc actggacact
    55381 gatcaatttg aacccaatga ccattagagt tggaatgatc cctgcttgta tccatacagc
    55441 aatcaatgaa ccgactaaaa taaaaataaa gattgggatg attcctggtt tgatgccatt
    55501 gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt atcactgcat acaacatgac
    55561 cattccgatc gctgctaaga tcgtgatatt aggtgagacc tttaatccga caacgccaat
    55621 cgcaatgcct aaaacaataa ccattaatac aagtgttgct tctaaaaaac ttggtgttcc
    55681 tttttttccc atgataatct ctcctttttt agttgttctt atgttctaaa aaagttaaga
    55741 tatcggatta ctccgccgca attcatccgc gattcctcct ttataaaaat aaaaagacct
    55801 gttttaaact aaggacgaat gactccgcgg taccacctta attaaaaaca gatctgtttt
    55861 tcactttcaa acttttattc aaatgtatgg actttccatg taattcgtca attaggcttg
    55921 atcggttcac agcgaccacc gactttctga acaaaacacc taaccgctac tgcgtgaaaa
    55981 gttgacttgt acgaagttta ccttccataa gatcatttgt caatgaaaaa gaaaatattt
    56041 tttcattttt ctgaaaatta tttttataac tttcacacac aagtttattt tttactagaa
    56101 agtagcgccc tcttgttttt ttcttgctac tatttcttga tgttcttgct tgattctttc
    56161 taatagcttt ggctctttca acagatcaag agctgtattg gcgagtagtt ctgctgcaat
    56221 cgcaatagaa gcaaggcctt tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc
    56281 ggcaatatat gtctcagaga ttgctaccgt aggctggatt gttggtatga cttggctgac
    56341 atttcctaca tcagaagaac cgagacttgt tctttgttcc gtttctattt cttcatctgg
    56401 aacacctgct tcttttgcat gctggaagaa taagtcatca aaagaaggag taacgatgac
    56461 atcatcaact gcattttgga acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc
    56521 acctcgtacg atattttcca ctttttcata tactttatct aatgtttgcc gatttcctgc
    56581 acgtaaatag aaacgtccag cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc
    56641 tgtaatgatc ccatggatat tgacatcttt tggcaattgc aagcgtagag cgttgattcc
    56701 attaaacact tggatcaatg cttccaaagc atttactcct ttttcaggtg ctgctgctgc
    56761 atgggaagcg acaccgtaaa atttgatgtc gactggatca ttcgctaaag attctgtcgt
    56821 tttcccataa cgaaaagctg gatggacgca taacgctaca tcgacatcct caaaaaaacc
    56881 ttctcgaaca aagctgcctt tagctgatcc gttctcgccc ccttcttccc ctggggtgcc
    56941 atatacacgg atttctccac cagtttcatc aatgaattgc ttcactatgc tggcggctaa
    57001 tacggagtaa gtaccaaaca aattatgccc gcatgcatga ccaatacctg gtaaagcatc
    57061 gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg gaaatataac gtgcatcaaa
    57121 ggctgttcgg tgtccagcta cttctttttc tacccaaaac ctttcttttt ctaattgttt
    57181 aatcaacgct gctgaggaat aaaactcata gttacttact tcaggatgtt cgtggatatc
    57241 tagagcaagt gcttgatatt ctggtaaacg atattggatt agatccttca attggtttgt
    57301 tgagattgtt gttgtcataa aaaattcctc ctatgctagt catttttctt attttctccc
    57361 gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt
    57421 gtcttctgtt tgataagcgt ctactatttt ttgatacgtt ttattgtttt catcttcttt
    57481 tcttgcaacg atgatattca cataaggacg tgcatggtca tccactggtt ctaagaagat
    57541 ggcatcagaa gcaggattaa atccagcatc tacagctaca ccgctattga tcactgaaat
    57601 gtctacatct gacaaagcac gtgctgtttg ggaagcatct aattcagaaa tgtctagatt
    57661 ttttctattc tcggtaatat cagaaacagt gggcgcttgt ccttttgcct tatccacttt
    57721 gatcaatcca gcagtttgca ataacagaag agctctgccg ccatttgtaa catcattagg
    57781 gattgctact tttgctccgt ctttgatatc agctacatcc ttcaaagtgt tagaataaat
    57841 tcccagcggt gcattgacag tatttccgat cgatactaaa tcagttccgt gttctttatt
    57901 gtaattgtct aaaaagaatt gatgttggaa agaattcaaa tcaatcgatc catctgctaa
    57961 agccacattt ggttgattgt aatctgtaaa tttcacgtat tccaaattaa tgttctcctt
    58021 tttcaaacgg tctttcacgt tgtcccatac atctgtatct tctccaataa tccctagttt
    58081 tacgatagtt tcttttttgt cagttgtatc tgcttttcct tggctacacc ctgccaacca
    58141 aataattgct acaaatgtaa tgactacaaa acgaataatt tttttcattt tttctcctcc
    58201 actttcaatg tgtaattttt ttaatcagtt gattactgat aaattgactt aaaaaaacta
    58261 agattagaat caataatgta gccacaatcg ttacatctgt ttggaatcga ttataccctc
    58321 tcgatatagc aagattccct aatccgcctg cacctactgc tccagccatg gtagtcagtc
    58381 cgatcacgtt gatgatagtc aacgccgaga cccgaatgat accaggcaaa ccttctaaca
    58441 aatacacacg taaaatgata ccgattggac tggttcccat cgcttctgcc gcttcgatca
    58501 ccccaggatc aacttctagc agtgcgttct cgatttgacg agcaaaaaac ggaacaactc
    58561 caaaaatcaa tggcactaaa gctgctttcg caccaatcgt cgttccaact aagaaacgag
    58621 tagtcaatgc taaaagagaa agcaagataa tgaaagggat ggaacgaatg atattgatca
    58681 gcttatcaag tatttcaaat agcagacggt tagctagtaa tccattaggg cgtgtcacta
    58741 ctaacgtcac acctaagatg attccaatca tcccgccaat gaatgctgtc caaaaagaca
    58801 tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg aatcaaataa acattgggca
    58861 aatatgtttt aaagaaatca gtcattttat cttcctcctt cgatcacttt gatacgacta
    58921 tctaaatcgt ttttttgtaa aatatttatt tttacaccag atttgatcaa ataatccaat
    58981 gcttttttcc gctgtttggt ttctccagct aaagtaacga tcaaactccc taacggcgtt
    59041 tcttgtaata actctacatt gccatataaa atattcgctg ttacttgata tttgctgtac
    59101 agatgagcga ttagaggttc gttcgtctga tttccaatat aacttaattc caccagccat
    59161 ttattggcga tctgatcggc aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca
    59221 agatgagtag ctgttcgaat aaaatctttg gtcaattctt cttcaggatt gctgaagatc
    59281 tggatgctgc tacctttttc aacgattctt ccatcttcca taaccgctac cttgttgcag
    59341 atttctttga caacttgcat ctcatgagtg atcaggacga ttgttaaacc taactggcga
    59401 ttcaattttt ttaacaatgc aaggatctga agggtcgttt tcggatcaag tgcactggtt
    59461 gcttcatcac ataataatac cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt
    59521 tgtttttgtc cccccgacag ttgtttagga taagcagatg ctttttcttc caaaccgaca
    59581 agttccagta gttcattcac tttttgacga cgttcctgtt tcgattttcc agaatacttt
    59641 aaagaaaaat caacgttgtc aaaaatagtt cggctttcca ttagattgaa atgctgaaag
    59701 atcataccga tggcctttct ttcttttcgc aattctttgg gagagagctg actcagattc
    59761 gtctgattga caatgacttc tcctgctgtt ggtttctgca gcaaattcat cacacgaact
    59821 aacgtacttt tccctgcacc tgagtaaccg acgattccat aaacatcgcc tttttcaatc
    59881 gttaaatcta cagaatctac tgcctgtatc tctttttctt tttgtttgaa acatacagaa
    59941 acatctcgca attcaatcat taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa
    60001 tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt
    60061 gttcacacac aactcctctt caagtacgat catactctag caagatatcg gtcgcagccg
    60121 tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt
    60181 ccttgcttct tctcagctac cgaagctctc tgtcaaaggt tcttctatcg tacttaccat
    60241 ttcaatgcaa ataatattca aaaaaaaagt ccttttacta acataaacta tgtcagtaaa
    60301 aggacgacgt atcgcggtac cacctttttt cggaagtttc cttccctcaa gcccaacatc
    60361 tcaaaaagag agattggcgt tcgttttaac ggtcgaacag ttccgaattt gcctacttat
    60421 cgacaaatta ttctccaagg ccattttcag cttttcttac ttgactcttc tcaccagcca
    60481 gagcctctct gcaaagtttc caaacttact ttccttttca acgaaatcga tattttgttg
    60541 ttgggattaa tctacatgag gaaaactgaa aagtcaagta cgaaattcaa attatttttt
    60601 ctatatttat ttttctgttt ttttatttaa catttcaata acagttcgaa gatcttttcc
    60661 ttccgcaaga agtttcaatt tttccttttc ttcgacggta taatttcgga caaaactgga
    60721 atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga
    60781 atcttccact gcaaaggctg aactagcttc taatcctgtt cgttccaaag ctgtcagata
    60841 aggaatcggt gaaggcttgt tctccgacac ttcgtctccg aatgtaaacg tgtccaagta
    60901 atcagctaat ttaaagtgat ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc
    60961 aatgcctagt ttcatatgac gttcttttaa gtctgccaat atttcttttg catatggttt
    61021 taagtggatg gctccgcttg tcagctgttg ataaaaatat tcttcacgct tcgctcggac
    61081 ttcctgtact ttttcagggc tacctgctgc tttcactaaa atatcaaaac tttgtttcca
    61141 gttttttccg ccccaagcgt caacgatttc ttttgggata tggatatcaa attgctgaaa
    61201 tccccaaagc cagccatcac gatacgtttt ttctgtgtca actaacaaac catctaaatc
    61261 aaatattgca cctttcatct atacattaat cctttccaaa acaatacaca tagtttagca
    61321 cgactggtcc aaaaagaaca gaagcaaaga accagccttc tcgtttataa gagttcttgg
    61381 aatcgacgga tatagatttt cttcacagct gtcacaagcg ccagataagc gatgactgta
    61441 gcaaataacc ataaccagta attgccagga agtggcatca agcctaattc agcaccaaaa
    61501 cttgtaaaag gaaggacaga accgacaata attcctaatg tagtgataac aaacatggca
    61561 aatgttgcgt tgctttggat aaatggtact ttcggtgtac gcagtgcatg gagcactaag
    61621 gtttgtgtcc atagtgattc gacaaaccat cctgcatgga ataatgcgat gaaaatcact
    61681 cgctgtgaac tatccaatgt atggaaactt ccgcccacta cagcaggaca aataacaaaa
    61741 tacatcaaca agtaagtagt aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg
    61801 aattccccaa tactagatgc attccatttt tttggctcgt ccaaatattc tttatccatc
    61861 cgatcccatg gcaaggaaat gcaggaaaca tcatagatca gattcaaaaa caaaagttgt
    61921 aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa tcagcacgga aaacatattg
    61981 ccaaaattag aacttgctgt agccttgata tatttcatga tattcccaaa agtttctcgt
    62041 ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca
    62101 gattctttcg caatatccac agctgtatct acagaaatac cgatatcagc cactttcatt
    62161 gcaggagcgt cattgatacc atctcctaaa aatccgacag tatgaccatt tttacgtaaa
    62221 gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta cgaatacttc atggtcttca
    62281 gctacttgtg ctaattcatg atcactcatt tccgagattt tctcaccggt aaccaattca
    62341 ttgatctcta gacctacttg acggcacact gaacgtgtaa ctaattcgtt gtccccagtc
    62401 agaactttta cagctacacc atgttctttc agagctttca acgcttgttt cgttgtttct
    62461 tttggtggat caaggaaagc aagataacca atcaaaacca tgtctgactc atctttgact
    62521 gaaaattctc caacgacgct tggattggtc ttttgtgcga caccaatcac tcgtaagcca
    62581 tcttcattaa gtgactgtac ttttgcaagt acggcttctt ttcgttcttt tgttaatggt
    62641 gtaacagtac cgccaagatc aataaattta gaaatgttca gcatttcttc aatcgcgcct
    62701 tttgtgatca tttgtgtttt accagctgga tcagcaacca caacactcat acgacgacgt
    62761 tcaaaatcaa aaggaatttc atccactttt tgataattga tcttatctgt atctaaagtt
    62821 tctgttgctt catcaatgat agccacatct aacaaatttt tcagccctgt ttgatagtag
    62881 ctatttagat acgcatggcg caaaacacga tcatcttcat ttccttcaca atccatatga
    62941 tattcaagaa tgattttatc ttgtgtcaat gtccccgttt tatccgtaca taacacatcg
    63001 attgcaccaa agttctggat ggcattcaaa tttttgatca ctgttccttt tttggccatc
    63061 acacttgctc ctttgaccaa attcgtcgta acgatcattg gaagcatttc aggtgttaga
    63121 ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc aatccccttt tgtaagccca
    63181 ttgatgacga taactgctgg agccatcaat gacataaatt tgatcagtag catggatgtt
    63241 ttatggatgc ctaattcaaa gcttgattga attggttttt cagatagcgt ttctgctaca
    63301 tgaccaaata gtgtttcatt tccaacagaa acaatgattc cttccgcact tccactgatg
    63361 acattacttc ccataaagac aagattttcg taacttgttt cgctttgctg atcgtcttca
    63421 catactttcg catgcttttc tactggatag ctttcccctg tcaatgctgc ttgggaaatg
    63481 aataaatcct ttgcggacaa taaacgcata tccgctggta tcatatcccc tgctgaaagc
    63541 cgaacaagat ctccacagac gatttcttcc ataggaatct ctgtgtactt tccattacgt
    63601 tttacagctg ccgttacttt cacaagtgat ttcagttttt cagccgcttg attggaacgt
    63661 acggactgaa tcaacgtcat tgtcccgctg acgataacca tagtgaagat aatggctaca
    63721 ccgaaaagat ctttatctcc aggagctgct aatacatatt ccgtgatgaa tgaaataatt
    63781 cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg
    63841 aatggtgttt ttttgccata tgaaatcgca ttttcaccgt aattttctct tgaacgttcg
    63901 atttgttctt ctgtcaatcc atttgctgat gcatggaata gttcaaataa ttcttgtttc
    63961 gtttttccag caaaagctgc atagattttt tgtgtatctt tctttttttc catctcaatc
    64021 tctcctttgt gtccaaaaga cagctgttct cgctgctttt ggtctttagc tattcatcgg
    64081 atcgatagag aaaaaactga aaggggctga gaacagatag ataatagccc tggcctttcc
    64141 tctagatcct tgtttttcga atgactgtca tccatttgtt ctcatctcct ttactaagta
    64201 attttaattc atgacttcca atcatttttc gaatagttat ttttccaatt agaccacctc
    64261 ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa aacctctcgc caaatactca
    64321 gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag atctcatcgt tgagttttga
    64381 cactgtacaa cgtaaaaagc agatacctgc ttttagctac cttatgaaaa gccttatctc
    64441 gacaattcct gttctaccca ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg
    64501 tataggagcc tcacctaaca aatcttattc ggttttcctt cgttaatata acaactaaaa
    64561 aagaggatgt caaatcaatt ttccatgttt ttcatacttt ttttaaaata cacacgttat
    64621 attcctgtct cacgcggttt tatttgtctc attttccacc tcccgctata atagaaatat
    64681 agtaggaaac gaggtgctgt tatggaaaga acacttgtta ttatcaagcc agacggcgtg
    64741 agacgccatt tagtcggaag catcatccaa cgttttgaag caaagggact ggccattgct
    64801 gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag aacattatca gcacttgact
    64861 gagcgcagtt tttttgatga actgatcgat tacatgactt ccggtcctgt cgtttatctt
    64921 gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga tggttggagc aacaaaagct
    64981 gccgatgctg ttccaggtac gatccgagga gactacgcgc tgccggggac tgaaaacatc
    65041 attcacgctt ctgattcccg tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca
    65101 ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat acaaattcta aactgctggt
    65161 ctagttgctg ggttcagcaa tggccagcag ttttttctct aaggacctcc gttgattctt
    65221 tcctaacata tattcgagca ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc
    65281 taaagcagca gaaatcaatc tttgataata atctgccatc aatgtttcat agatcactgt
    65341 acttctttct ttatgtgcta agctccgttt catatcttca ttcacactac gtgccttttt
    65401 caaccgacca ttttctatat aaaacgaagt caggttcatt aaaaaagtaa agagatactg
    65461 ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt ttgattttca aataattgtt
    65521 ctcaatgaac cgatcactga atatccaagg aaaatcagct agaatagata gttcaaattg
    65581 accatagtct ggtatctgca tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa
    65641 ttcaaattct gtctcagata cttctgtttt ttcgaccatg tgaagattca aaaccaaata
    65701 atacagatct tctgttttct taaaatgcct acggttattg ctgatctctt tataaggatc
    65761 aaagctgtct tcttgtaccc gatctagcat tcgtcttttt ttctttaact ttattggttc
    65821 atccagctgg tttcgcatcc attcatattc tgcaagtgtc atgttcattc gctctaaaaa
    65881 tgatctcaat gttacaaatg acattttttt cactgaattt gtctcaatcc cgactagaga
    65941 agtcttaaaa cagattcctt ccgctaattg ggactgtgtc aacttcttgt tgactcgaag
    66001 tttcttgatc aattcaccat catacataat agttctcctt tttaatttaa atttttccac
    66061 attcaataaa tagatacgtt aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt
    66121 ttcataaaaa ataccaacct tcaagcatta gaatgttgtc taactactga aggttggttt
    66181 gattgtttat tgattatttt tttgctgttc aacgtctgtt cttgttactg gatataagtt
    66241 ggttgttgtt ttattaccat tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt
    66301 ttcgattgct tcattacgtt ccgcttcatt tttatagtca tattttttcg gatcaatcgt
    66361 ctcaaatccg tttggcacat aaaaccgcaa gagattttcc tgattcagct tatctgaaag
    66421 acttaacgca gtcttgactt tttcctgatc tttggagatt tcctcatcga ttcctgctgc
    66481 tttcgtatcg atgatttctc ctgtctgatt attgtaagct tttcctccta ggacggtgta
    66541 tttgggtgtc acaaagttcc catttcgaaa agcaactact tgatcatgct gagaagataa
    66601 taagtctgta ccaaaatgaa gataatcttt atcatcgatt cccaatagat gtagtaatgt
    66661 cggcaaaaca tcgatttctc caccgtattc atgattgact gtccctttag tataaccagg
    66721 catatggatc atcaatggca ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc
    66781 tggatcttta ccaagagcgc ttgctaaatc tttattatct gtattcgaga tcccaaaatg
    66841 atccccgtaa atcacaaaga ttgaattttg gtaaatacca gattttttca aatacgtaaa
    66901 aaattgttcc aatgattgat ccagataatg tgccgttcga acataattat ctactgtgct
    66961 attgccagta ttcaaagatg gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg
    67021 gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc
    67081 agattctctg aataaatctt tatctaatat cccataacct aacgtttcgt cggattcatc
    67141 aaaataagat cgatcaaaga aattgtcata acctaagttt ttatatacat gatctctatt
    67201 ccaaaaacta gctacattcc catggaaaac agcgcttgta tagccttgct tttgtccaag
    67261 gatcgctggt gcggcttgaa agacattgtc tgacccaagt tctgtgaata atgagccttg
    67321 aggtaaacca aatgtgccag tttccaacat attttcagca tcacttgtct ttccctgtcc
    67381 tacttcatga aagaaattgt caaagctgat cgttgcttga ttctggaaaa tactatttaa
    67441 aaacggtgtg acttcttgtc cgtctacctt catattaatc aaaaactgct ggaaactttc
    67501 taaatggaga acgatcacat ttttcccttt agcgatccca aatgtttcgg gatttggctc
    67561 tgcataatgt ttctttgtat aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc
    67621 gcgaacactg ctagtcattc ctgttttgat tccgtcataa atagtatacg catcgattcc
    67681 tagatatttg acaatatatg aacggtcaaa tgttcgctgc agcaattgtg gacggttggc
    67741 ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc
    67801 catcggtttg ccgacagggt tgcttttgat aggtattttc ttgaccttca accaaaccag
    67861 tagcccaatg aacacagcga tatccagcca gtagatgaca tcatggggct tcatcaaaga
    67921 aaaggagctt cctgacaatc cttgcgacac tttcgaaaag cctaatacag atttgactgt
    67981 aatgaaatct gtgaattccc gataaaaaat aatattcaag tacaacaaaa gcgtatttaa
    68041 aatatcaatg atcaaaagaa tagctaacgc tggctttggc tttttaatat acaaagaaag
    68101 gccaaaaaac aataaggtag tcgcaattgg gttgatccac aagataaagt actggatcgt
    68161 accttccaca cctaatgaaa aatctgcata gtaggcaatg atcgtcttca gccagaaaaa
    68221 gaaaacagct gtcaaaacga ttgctaaacg attttggcac aattttttaa gttgtgtcac
    68281 gtatatcact tctctctatt cacatttttt cttattttgc tcctgaaaaa tgaaaatttc
    68341 ctaaaaaagt tgttatctta agattttttt attctttcta aagattattc cgtccacttt
    68401 tcacagcata aaaccgctgc gatgtaagcg attaaactaa tgaagagata aaagtcttgt
    68461 ggtgaccgct tcactccacg gtattcaaaa gccagtttct cggtattcgt acaaaaaaag
    68521 aggctgtgac aaaattcttg tcgcagctcc ttatttcttg atgcaggaca gctttttcac
    68581 aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg ccacaagtca caatatttat
    68641 aaaacatgag aagctgtttt ttgaaattgt tccttgtggc tcagagttaa acggcaggct
    68701 caagacctct tgatacacgg tgttcaatca gctgaccctc ggtattagat tatgtcagga
    68761 cgctaatttt acaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc
    68821 ccaaccaaac gaaaaatata aggagctatt ttttgaaatt gctccttgtg gctcagagtt
    68881 aaacggcagg ctcaagacct cttgata
//
LOCUS       DACCDF010000006        63318 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCDF010000006 DACCDF010000000
VERSION     DACCDF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.006"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            121..984
                     /locus_tag="IVK51_000336"
     CDS             121..984
                     /locus_tag="IVK51_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6429958.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1113..2735)
                     /locus_tag="IVK51_000337"
     CDS             complement(1113..2735)
                     /locus_tag="IVK51_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6429959.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2984..3505
                     /locus_tag="IVK51_000338"
     CDS             2984..3505
                     /locus_tag="IVK51_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429960.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3691..5421)
                     /gene="spxB"
                     /locus_tag="IVK51_000339"
     CDS             complement(3691..5421)
                     /gene="spxB"
                     /locus_tag="IVK51_000339"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP6429961.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5586..6404
                     /locus_tag="IVK51_000340"
     CDS             5586..6404
                     /locus_tag="IVK51_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP6429962.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6507..6704)
                     /locus_tag="IVK51_000341"
     CDS             complement(6507..6704)
                     /locus_tag="IVK51_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429963.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6873..7451
                     /locus_tag="IVK51_000342"
     CDS             6873..7451
                     /locus_tag="IVK51_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6429964.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8496..8999)
                     /locus_tag="IVK51_000343"
     CDS             complement(8496..8999)
                     /locus_tag="IVK51_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6429965.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9357..10382
                     /locus_tag="IVK51_000344"
     CDS             9357..10382
                     /locus_tag="IVK51_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6429966.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLVGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10705..11583
                     /locus_tag="IVK51_000345"
     CDS             10705..11583
                     /locus_tag="IVK51_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429967.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12059..12175
                     /locus_tag="IVK51_000346"
     CDS             12059..12175
                     /locus_tag="IVK51_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6429968.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13103..13441
                     /locus_tag="IVK51_000347"
     CDS             13103..13441
                     /locus_tag="IVK51_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429969.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13535..13765
                     /locus_tag="IVK51_000348"
     CDS             13535..13765
                     /locus_tag="IVK51_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6429970.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14165..14536
                     /locus_tag="IVK51_000349"
     CDS             14165..14536
                     /locus_tag="IVK51_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP6429971.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14810..15502)
                     /locus_tag="IVK51_000350"
     CDS             complement(14810..15502)
                     /locus_tag="IVK51_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6429972.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15505..16290)
                     /locus_tag="IVK51_000351"
     CDS             complement(15505..16290)
                     /locus_tag="IVK51_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6429973.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16527..17927
                     /gene="lacG"
                     /locus_tag="IVK51_000352"
     CDS             16527..17927
                     /gene="lacG"
                     /locus_tag="IVK51_000352"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6429974.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17952..18269
                     /locus_tag="IVK51_000353"
     CDS             17952..18269
                     /locus_tag="IVK51_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6429975.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18282..19955
                     /locus_tag="IVK51_000354"
     CDS             18282..19955
                     /locus_tag="IVK51_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6429976.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19969..21600
                     /locus_tag="IVK51_000355"
     CDS             19969..21600
                     /locus_tag="IVK51_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP6429977.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22091..23656)
                     /gene="guaA"
                     /locus_tag="IVK51_000356"
     CDS             complement(22091..23656)
                     /gene="guaA"
                     /locus_tag="IVK51_000356"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP6429978.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23715..24638)
                     /locus_tag="IVK51_000357"
     CDS             complement(23715..24638)
                     /locus_tag="IVK51_000357"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP6429979.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24834..25439
                     /locus_tag="IVK51_000358"
     CDS             24834..25439
                     /locus_tag="IVK51_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6429980.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25747..26763
                     /locus_tag="IVK51_000359"
     CDS             25747..26763
                     /locus_tag="IVK51_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6429981.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26904..28205
                     /locus_tag="IVK51_000360"
     CDS             26904..28205
                     /locus_tag="IVK51_000360"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP6429982.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28251..28913
                     /gene="deoC"
                     /locus_tag="IVK51_000361"
     CDS             28251..28913
                     /gene="deoC"
                     /locus_tag="IVK51_000361"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP6429983.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIVETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            28934..29332
                     /locus_tag="IVK51_000362"
     CDS             28934..29332
                     /locus_tag="IVK51_000362"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP6429984.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29398..30480
                     /locus_tag="IVK51_000363"
     CDS             29398..30480
                     /locus_tag="IVK51_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6429985.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30692..32254
                     /locus_tag="IVK51_000364"
     CDS             30692..32254
                     /locus_tag="IVK51_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6429986.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32247..33380
                     /locus_tag="IVK51_000365"
     CDS             32247..33380
                     /locus_tag="IVK51_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429987.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33380..34336
                     /locus_tag="IVK51_000366"
     CDS             33380..34336
                     /locus_tag="IVK51_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6429988.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34435..35601
                     /gene="deoB"
                     /locus_tag="IVK51_000367"
     CDS             34435..35601
                     /gene="deoB"
                     /locus_tag="IVK51_000367"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP6429989.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35625..36443
                     /locus_tag="IVK51_000368"
     CDS             35625..36443
                     /locus_tag="IVK51_000368"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6429990.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36460..37167
                     /gene="deoD"
                     /locus_tag="IVK51_000369"
     CDS             36460..37167
                     /gene="deoD"
                     /locus_tag="IVK51_000369"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP6429991.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37465..38151)
                     /gene="gpmA"
                     /locus_tag="IVK51_000370"
     CDS             complement(37465..38151)
                     /gene="gpmA"
                     /locus_tag="IVK51_000370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP6429992.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38323..38904)
                     /locus_tag="IVK51_000371"
     CDS             complement(38323..38904)
                     /locus_tag="IVK51_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6429993.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39037..39720)
                     /gene="rpiA"
                     /locus_tag="IVK51_000372"
     CDS             complement(39037..39720)
                     /gene="rpiA"
                     /locus_tag="IVK51_000372"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP6429994.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40039..40452
                     /gene="rpsL"
                     /locus_tag="IVK51_000373"
     CDS             40039..40452
                     /gene="rpsL"
                     /locus_tag="IVK51_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP6429995.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40536..41006
                     /gene="rpsG"
                     /locus_tag="IVK51_000374"
     CDS             40536..41006
                     /gene="rpsG"
                     /locus_tag="IVK51_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP6429996.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41100..43187
                     /gene="fusA"
                     /locus_tag="IVK51_000375"
     CDS             41100..43187
                     /gene="fusA"
                     /locus_tag="IVK51_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP6429997.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43359..44546
                     /gene="tuf"
                     /locus_tag="IVK51_000376"
     CDS             43359..44546
                     /gene="tuf"
                     /locus_tag="IVK51_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6429998.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            44953..45261
                     /gene="rpsJ"
                     /locus_tag="IVK51_000377"
     CDS             44953..45261
                     /gene="rpsJ"
                     /locus_tag="IVK51_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP6429999.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45292..45921
                     /gene="rplC"
                     /locus_tag="IVK51_000378"
     CDS             45292..45921
                     /gene="rplC"
                     /locus_tag="IVK51_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP6430000.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            45949..46572
                     /gene="rplD"
                     /locus_tag="IVK51_000379"
     CDS             45949..46572
                     /gene="rplD"
                     /locus_tag="IVK51_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP6430001.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46572..46862
                     /gene="rplW"
                     /locus_tag="IVK51_000380"
     CDS             46572..46862
                     /gene="rplW"
                     /locus_tag="IVK51_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP6430002.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46901..47734
                     /gene="rplB"
                     /locus_tag="IVK51_000381"
     CDS             46901..47734
                     /gene="rplB"
                     /locus_tag="IVK51_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP6430003.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47777..48055
                     /gene="rpsS"
                     /locus_tag="IVK51_000382"
     CDS             47777..48055
                     /gene="rpsS"
                     /locus_tag="IVK51_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP6430004.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48078..48425
                     /gene="rplV"
                     /locus_tag="IVK51_000383"
     CDS             48078..48425
                     /gene="rplV"
                     /locus_tag="IVK51_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP6430005.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48439..49095
                     /gene="rpsC"
                     /locus_tag="IVK51_000384"
     CDS             48439..49095
                     /gene="rpsC"
                     /locus_tag="IVK51_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP6430006.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49098..49532
                     /gene="rplP"
                     /locus_tag="IVK51_000385"
     CDS             49098..49532
                     /gene="rplP"
                     /locus_tag="IVK51_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP6430007.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49522..49710
                     /gene="rpmC"
                     /locus_tag="IVK51_000386"
     CDS             49522..49710
                     /gene="rpmC"
                     /locus_tag="IVK51_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP6430008.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49735..50001
                     /gene="rpsQ"
                     /locus_tag="IVK51_000387"
     CDS             49735..50001
                     /gene="rpsQ"
                     /locus_tag="IVK51_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP6430009.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50059..50427
                     /gene="rplN"
                     /locus_tag="IVK51_000388"
     CDS             50059..50427
                     /gene="rplN"
                     /locus_tag="IVK51_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP6430010.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50464..50772
                     /gene="rplX"
                     /locus_tag="IVK51_000389"
     CDS             50464..50772
                     /gene="rplX"
                     /locus_tag="IVK51_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP6430011.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50800..51339
                     /gene="rplE"
                     /locus_tag="IVK51_000390"
     CDS             50800..51339
                     /gene="rplE"
                     /locus_tag="IVK51_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP6430012.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51358..51543
                     /locus_tag="IVK51_000391"
     CDS             51358..51543
                     /locus_tag="IVK51_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP6430013.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51579..51977
                     /gene="rpsH"
                     /locus_tag="IVK51_000392"
     CDS             51579..51977
                     /gene="rpsH"
                     /locus_tag="IVK51_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP6430014.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52009..52545
                     /gene="rplF"
                     /locus_tag="IVK51_000393"
     CDS             52009..52545
                     /gene="rplF"
                     /locus_tag="IVK51_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP6430015.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52706..53062
                     /gene="rplR"
                     /locus_tag="IVK51_000394"
     CDS             52706..53062
                     /gene="rplR"
                     /locus_tag="IVK51_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP6430016.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53083..53583
                     /gene="rpsE"
                     /locus_tag="IVK51_000395"
     CDS             53083..53583
                     /gene="rpsE"
                     /locus_tag="IVK51_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP6430017.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53598..53777
                     /gene="rpmD"
                     /locus_tag="IVK51_000396"
     CDS             53598..53777
                     /gene="rpmD"
                     /locus_tag="IVK51_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP6430018.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53821..54261
                     /gene="rplO"
                     /locus_tag="IVK51_000397"
     CDS             53821..54261
                     /gene="rplO"
                     /locus_tag="IVK51_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP6430019.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54261..55556
                     /gene="secY"
                     /locus_tag="IVK51_000398"
     CDS             54261..55556
                     /gene="secY"
                     /locus_tag="IVK51_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP6430020.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55618..56265
                     /locus_tag="IVK51_000399"
     CDS             55618..56265
                     /locus_tag="IVK51_000399"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP6430021.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56456..56674
                     /gene="infA"
                     /locus_tag="IVK51_000400"
     CDS             56456..56674
                     /gene="infA"
                     /locus_tag="IVK51_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP6430022.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56707..56823
                     /gene="rpmJ"
                     /locus_tag="IVK51_000401"
     CDS             56707..56823
                     /gene="rpmJ"
                     /locus_tag="IVK51_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP6430023.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56841..57206
                     /gene="rpsM"
                     /locus_tag="IVK51_000402"
     CDS             56841..57206
                     /gene="rpsM"
                     /locus_tag="IVK51_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP6430024.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57234..57623
                     /gene="rpsK"
                     /locus_tag="IVK51_000403"
     CDS             57234..57623
                     /gene="rpsK"
                     /locus_tag="IVK51_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP6430025.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57703..58641
                     /locus_tag="IVK51_000404"
     CDS             57703..58641
                     /locus_tag="IVK51_000404"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP6430026.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58669..59049
                     /gene="rplQ"
                     /locus_tag="IVK51_000405"
     CDS             58669..59049
                     /gene="rplQ"
                     /locus_tag="IVK51_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP6430027.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59321..60160
                     /locus_tag="IVK51_000406"
     CDS             59321..60160
                     /locus_tag="IVK51_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6430028.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60136..61005
                     /locus_tag="IVK51_000407"
     CDS             60136..61005
                     /locus_tag="IVK51_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6430029.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            60995..61792
                     /locus_tag="IVK51_000408"
     CDS             60995..61792
                     /locus_tag="IVK51_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6430030.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            61995..62741
                     /gene="truA"
                     /locus_tag="IVK51_000409"
     CDS             61995..62741
                     /gene="truA"
                     /locus_tag="IVK51_000409"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6430031.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT        20069 a        11306 c        13494 g        18449 t
ORIGIN      
        1 gtcacaacct cttattttga ttagaacttg cgaaaataaa ccggttaaag tgtgtctagc
       61 gcaagagatt tgctataata gacgataagg gtaaaatgta acgagaaggg gttgaaaaat
      121 gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat accatgtatt agcaaaagaa
      181 aaagttgatt ttttatcaat gaatctccaa ttgaatgatc aagagttagt agatgacctc
      241 ggaaagacat tcaattatga ccgctacatg aatcagctga aaaatggtgc gatgcctacg
      301 acatcacaaa tcaatgttgg ccgatattta gaatttttcc gttcttatgc ggaaaaacag
      361 attcctgttt tatatgtggc tttttccagt ggattaagcg gctcttacag tagtgcgctt
      421 caagcggttg aattgctgaa ggaagaatat gaagaccctg agatctatgt ttttgataca
      481 aaagctgcaa gtattgggga agggctttta gtattggaag ctgtaagatt gaaaaaagaa
      541 ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta attatttgaa agtccattca
      601 tgggtaacag tagacgatct gaaatatcta gaacgaggcg gtaggatttc caaagcaagt
      661 gctactcttg gcagtctatt gagtgtaaag cctattatta cggtggataa agaaggaagg
      721 ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga tccagacaat cgtcaatgag
      781 acggtaaaaa caatcgttgc accacttgat caaatcgttt atgtggctta cgctggggat
      841 ctagagagcg cggagaaggc aaaacggctt ttggaagagc agataaagat gaaagatgtg
      901 aagctttatc ccttaggacc aacgatcgcc agtcacacag gctatggctg tatcgcgatt
      961 ttttcgatgg gaatcagtcg ataaaagcag taactcattc tgtaaaaaag cccagaaatt
     1021 gattgtaaca aaaacagcga ctcttaaaag atagatgagc atcctatctc ttagaggtcg
     1081 ctgttttttt attttttaag ctaaataggc cattatgccc ataactcgtt caattgtttt
     1141 tgaatatctg cattttccaa aaattcatca tatgtcgtat cagctcgatc gatcacacct
     1201 ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga taaattgatg gtcatgtgac
     1261 gcaaataaga ttgagccagt aaatgccatc aagccgtcat tcaatgctgt gattgattct
     1321 aagtctaaat ggttcgttgg atcatctaat acaagtacgt tagctttcga aagcatcatt
     1381 tttgaaagca tgcaacggac tttctctcca ccagataaaa cattcactgg tttcaatact
     1441 tcttcaccag aaaacaacat tcttcctaag aagcttctta agaaggtgtt atcatcttct
     1501 tctttacttg catattgacg caaccagtct aaaatcgtta gatcagtatc gaattctttc
     1561 gttgtatcct ttggtaaata agaattactt gttgtcacac cccaacgtac tgttccagta
     1621 tctggcgtaa tttcacccat gataactttg aatagcgttg ttgttgttat atcattttct
     1681 gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag atatatcatc taaaattttc
     1741 tttccatcaa tcgttacact tacattttct acctgcaata agtcgtttcc aatctcacgt
     1801 tcaggtttga atccaacgaa tggataacgt ctagaagaag gttggatatc gtctaaagta
     1861 attttttcaa gcattttttt acgtgaagtt gcttgttttg attttgatgc attcgcacta
     1921 aagcgggcaa taaattcttg gagttctttg atttgttctt cttttttggc atttgcgttt
     1981 gcttgaagtt tagccgctag ttgactagat tccaaccaaa aatcataatt tcctacataa
     2041 agtttgattt taccaaaatc tagatccgcc atatgtgtac atactttgtt caaaaagtga
     2101 cggtcatggg atacaacgat cacagtattt tcaaaattga tcaaaaattc ttccagccaa
     2161 ttgatcgatt ggatatccag accgtttgtt ggttcgtcta gcaaaagaac atcaggttta
     2221 ccaaatagtg tttgtgctaa taacactttt accttttgtc caccagttaa ttcactcatt
     2281 tgctgatggt gcaattcttc agggatgttc agaccttgca gtaaaacagc tgcttctggt
     2341 tcagcttccc atccattaag ctcagcaaat tctccttcta attcagctgc tcgaatacca
     2401 tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt tttctttcag cacttcatac
     2461 agacgcttgt gtcccatgat gactgtttca agtacagtat agtcttcata gtcgtaatgg
     2521 ttctgtttca atacggccat ccgttcatct ggtcccatag taacggaacc tgtggaaggc
     2581 tgcagatccc ctgataacac tttaaggaac gttgatttac ctgcaccatt tgcaccaatc
     2641 aagccatagc agtttccggg ggtaaattta atattcacat catcaaatag tttgcggtct
     2701 gaaaagtgca aactcacatc atttacagta atcaattttt ttcctcactt ttatcattat
     2761 tagttttgac aagtcttata gaattatata ggggaaaggt aaaagattca aggcaatcga
     2821 acattctttg tgtttcacgt gaaacagaat gaaaaaaata aaaataaaag cggagcgaaa
     2881 atagctacta taggatttat tagtttgcaa cagcataatt tttgacgtta tttataagag
     2941 ggagtactat aaaactgtaa aagaaatgaa agaaggattg aaaatggatg aaaaaagaac
     3001 catcgactgg ggctcattga ttttgggaat tttatttgta cttgtctcat tgttatcatt
     3061 tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc tttgcgattt ttgcctttat
     3121 taaagggata tttgaactgt ttgtacggaa cagattaaaa gagctaacag gatataaagg
     3181 aaagatgccc ttaataatcg ggatcatcga tattctagtt ggcgtctttt tcttattcaa
     3241 tattggtgca ggagtcgcag cattgccttt tgtttttgca gtatggttta ttgctgattc
     3301 agttttagcg ttatttactg ctgacttggc aagaggagta agtgaaggat attattggtt
     3361 tacgatcatc gtcaatattt taggaatttt attaggaatt ttcttattgt ttaatccaat
     3421 ttcttctgcg ttgacattaa gctttttagt tggattctat tttatgttat ttggtatcac
     3481 acatatcgtc tatgcattta gataagtaaa atcaaacaaa taaaaaagcc ggcttccgat
     3541 tacagtttta ctgttgtaat tggaagccgg cttttttatc atggacaaat atgcacgagg
     3601 tgatttttag gtgtcgagac aaatttatgc aaaaatgtga gaagctatat acacaaattt
     3661 tatcttaata ctcgaagcat agcgcagttg tcacaacctc ttttcatata attcattcaa
     3721 tactggcaaa tcatggactt catatttttc tttgaatgct tggatttctt cttcagaata
     3781 ttttcttgga tctaaatgca gatcttctac tggcaatggg cgcttgttat tgatcttcac
     3841 atcgattact acaggacgat ctgattttgc agctgcatca aaggcaaatc gaagttgcga
     3901 gtattctgtg actgtaaagc tatttgcccc gagcgcttct ccaactttac cgaaatctgc
     3961 atcttctaaa aatacaccaa atttcttttg ttccgtatct tcttgttctg cttcgatgaa
     4021 accaaaagaa tcattcgaaa agacaacatt aataattgga agctgatatt tgacttgtgt
     4081 aatgatatct tgcatgacca tagcaaaccc gccatcacca cttaaggtaa atacttgttt
     4141 ttctgggtag cttagttgtg cggcaattcc tcctggcaca ccattaccca tagttgcgaa
     4201 ccatccagat gtcgtatgtt tttgtttacc gttcatattc aataaacgaa tagaatggat
     4261 agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct tcagcaatac gattgatttc
     4321 tttatagaca gcctctggac gaatcggttc ttcctctcga tcttcaaaag atttcaacca
     4381 atttacccat ttttctttat tttcttgatt tgctaggtac cagctatcag cagggcgagc
     4441 atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca tcaccgagga tcgacaaaga
     4501 tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg atttggataa attcagcagt
     4561 tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg acaaataaga tcagatcggc
     4621 ttctgccaat gcttcatttc cgggtttagt agcgacacgt ccagcaaaac caagaaagtt
     4681 ttcataatca tcaggaataa ttccttttgc tagaacggaa gcgacaacag gaactgaaaa
     4741 atgttctgag aattctttga tttgcggaaa tccgtttcgc gttccttgac caacataaag
     4801 aactggtttt tttgcttttt caagataagg caaagcagct agcaaatctt tttcttctgg
     4861 taatatcaca cttgttttgt gagtgtgagc ggtggagaaa ggttgttcgt caatttcttc
     4921 aaaaccaaga tcaacaggga tagtcacgat agccacgcct ttatgctcat aggcggcttt
     4981 aatggcttca tctactacat gcggaagact ttgtggtgtc atgactgtac gattgtatac
     5041 gctaacatct gcgaaaatag gattttcatt tagttcctgg aaagagttat agttcatgga
     5101 tgtagaagca acttgtccta agaacgctac tagaggcaca tggtccatct gtgcatcata
     5161 caaaccattg atcaaatgtg tcgcaccagg tcctgcagag ccgaagacag cgccgacttt
     5221 ccccgttaat tttgcatctg cagcggctgc gagtgcgccg acttcttcat gacgaacttg
     5281 aatgtagttg atttcggttt tttcttgata tagcgcatcc ataattgaat tgaaagatcc
     5341 tccgggaatg ccgtaaatat ggtcgatacc ccagctttca agaactttta ccattgcgat
     5401 tcctgcattg atttttgaca tggtgtttca ctcctttagc aatgatgata cataaatttt
     5461 ttgtatctag agtatagaac tttttatcga aaatcccaac taattcgtct tttcacaaaa
     5521 atgtagtcat gtttttggga atatgctaga gtagtacata gatagattca agagagggga
     5581 atggcatggt tgaatggcta ttgagacttg aaccttggca gcaagcactt gctgggacag
     5641 cttttactta ttttatgaca gctcttggtg ccgggttggt ttttttcttt aaagaaatca
     5701 aaaaagaagt gctaaacttg atgttaggat ttgcttctgg tgtcatgatt gctgcaagtt
     5761 tttggtcttt gttagatccg gctattacaa aagcagaaga aaatggagat attgcttggc
     5821 tagttgtaag tattgggttt ggtttaggcg gtgtgttttt atatatggca gataaaacac
     5881 tgccccatat gcattttgga ccacaacatg aagcagaagg attgcctacc catttaaaac
     5941 ggacgatctt gcttgttttt tctattacat tgcataacat tcctgaaggt ttagccgtgg
     6001 gggtagcatt cggtgcagca gcaactgcgg ataatccgac agccgctata ttagccgctg
     6061 tttcggtggc gctaggtatt ggtatccaaa acttcccaga aggagcagct gtatcaatcc
     6121 cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt ttacggacaa gcttctggaa
     6181 tcgttgaacc aattgctgga gtgattggag cgctactagt aacgaaagtg gagctgctgc
     6241 ttccttatgc acttgctttt gccgcaggag caatgattta tgtcgtggtg gaagaattga
     6301 tccctgaagc acagcagaca ttgtccagca aacgtcattt tgcagttttt ggcgtcatga
     6361 gtggatttat cattatgatg attttggatg tggcattagg ataaataaaa aaaatatata
     6421 tgatatatca aatagggccg cgaacttata tcgcgaccct atttttttag taagttatat
     6481 cataatgaag tgaatggtat catacattaa taatagtttt ttctatgtgt atttcctttg
     6541 atccagcgaa caagccagat gataagtgcg attgggatta gaactttgac cgcaaaccat
     6601 aaaagcccgc ttaggacact gacaaaaaga ctgatgacga agctagctgc aattagcact
     6661 aaaagaacca tgaagatatt taagaacata tttttttgac gcatacaaaa ccctcctctt
     6721 gttcattctt tactatagaa aaagtataac agggattgtt taaaaacacc tccaactgaa
     6781 gactgatttt tacttcatct acttaaatga tcaaacaatt gctttttctc tggtttatca
     6841 tctaaaatag agcggaaaag gaggagaata tcatgcctaa aaatcgtctt gaagcattta
     6901 cagatgctgt aatcgcgatt atcatgacta ttttagtttt agaacttcat gaacctaacg
     6961 gagacacgtg ggaggcgctc gctgcattag gttaccgatt ttttatctat attgtcagtt
     7021 ttatcatttt agcgatttat tggaacaatc atcatcattt atttcaagtt gtacataaga
     7081 taaatggacg ggtcttatgg gcgaacagtt tctttgtttt tgcgctttcg ctttttccgt
     7141 ttgcaacttc atggatcagt gagtacatca atagcctggc tccagaaatg acttatggtt
     7201 ttgttacctt gaccgctaat attgcgtatt atatcttggc aaaagaactt gtcaaagcaa
     7261 ataaaggcca cgagaaagca ttgaaggcga tgttgaataa ttacaagaaa tcgtatctat
     7321 cgattggttt gaatgtgtta ggaattgtct tagggtattt agtagcgcca gtagccgtgc
     7381 tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc aagtcgtacc atcgaatcac
     7441 aatatcggta aaatgatccg tgtttaaaat ttaagagaaa aatatagaaa aaataaataa
     7501 acgatcaaat atccgagtga tactagtatt caaagaggga tgcagatgga tcaaaaaaga
     7561 attcatgtag gactgattac tagttttgtc acgatgctta tttatttgga tcaaggtcat
     7621 ttgacttgga cagtagttgg tgtcgtagga tttttgacat tattcacagc aataaaaaaa
     7681 taaacatctt tatgtaatct gatgttctat ctatttaaga aaagcatgtt tacagcagta
     7741 tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta atttttagaa aagtgtatcg
     7801 aaaaatagat agttagtttt tccacagtgg ataacttcgt aagagataaa aataaaaaat
     7861 tagtcaaaga agggcgtata gatttctttt gataggagat atacaacaga aatgaataaa
     7921 tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt tggaataaaa aaataacata
     7981 tctcatttta cacttttgtt attttatact taaaaaataa cgacacagaa aataccgtgt
     8041 ctcagatttc tctactactt ctaaaaacta ctgttttttt cctttgaaat gccttcaaaa
     8101 agtaggatca ggcagaatat actggcagaa tcaaggagaa gggagcctaa ataaggagaa
     8161 aaaccataga aaatataaag taattactta taaaacaagc tttttataga aaaataatat
     8221 acctgagtag ggtataaatt aagacggaat tcctttctag attgagatat tgactaggaa
     8281 gaatgttaag attttaagcc tttaagctaa aaaaaaaccc tttttgttaa ataaggggaa
     8341 tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca ataatatagt agattagaga
     8401 tgaaaataga tttttttaaa ccaatgactg aataaaaaga ccgtcataga tacacattgg
     8461 aatgtgtcta ttacggtctt tttttacgaa cgattttatt gaagacggac agttcgggtc
     8521 aaaaataaaa aagctacgat cagaaataag aataaaatgt atcccaaaat aatggctaga
     8581 cggtctaact tatttgtcga tagatgtttg tttagaagtt gataggccca taaacctatc
     8641 agtcctcctg ttgtattagt tatcaaatca gtgatgtccg ctacgccaat accgaatagg
     8701 aattgaatca attctgccaa gaagcttaca aggaaaataa tccctaattt tttagaaaaa
     8761 gaaagtctct tgaaattgac attcaaaagc accccaaaag gaaaaaagac aatcaagtta
     8821 taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa aagtagaaaa agggacgaga
     8881 ttgacattgc tgtaagaata agtgagaaat tgtggaagat taaatgatag cttgaataaa
     8941 atcaaccata atagaaagaa taaataaatc cctaataaaa attttgatac ataccgcata
     9001 gacatctcct ccatttaaaa agtataccat cgaaaaccgc tgaaaaatca taaaaaagta
     9061 tgaagtttaa ggaccaacgt agttatcgta cgaaaattag aagctttttt tgttgggaat
     9121 ccggtttctt taacaaagct aagatagcga gttgcttgga aaagttgcta tcttttttct
     9181 gtcaaaacga ctcggaatgt tcgttttttg ttctaatttc tccaagttgt ttactttttg
     9241 atactaagtt tatgaaaaat caagaaatag ttgttatact aggtaatgct gacaaacggg
     9301 attggcattt catcttggtt cagctttttt tccaattact aggaaggagg gatagattgt
     9361 tcaagactta ttttcgtttc aataaaatac aagataatcc ttttcgtatc atgaatctga
     9421 tgctacttat ctttattgta ttatggttag gttacataaa tcatgatttt ttgatttcaa
     9481 gtttttcatc attagggatc tttacttttt tctattatca aaacataccg atgaaatatt
     9541 tgctgaaacg tatgtttctg gtcggttgcg gactgttggt tgctttcgtt ttggggatct
     9601 tatcgactta tgttttatgg cttgaaccat ttgccgtagc tttggttgct ttttccagca
     9661 gattcgtgtt gcgattattc catatttcta aaccaggtgg cttgtttttt gcgatgcttt
     9721 ctgccatggg aacgagcatg cagctgccaa tcgctcaatt accgatcgtc tcactttatt
     9781 tttttatggg tgtcgttttt gccctgattg cggcagtgat tacaaaatta ctggattcac
     9841 ggcctgagca gacaattgaa aaagcaactt taaaagaaag atttcacgaa gagcctttag
     9901 tgatcattga ttctgttttc tacagcgctg ccttatttct ttccgtttat gtcagtcacg
     9961 gcttgaatct gcataatcct tattggttga ccttgtcatg tgcatcgatt ttgctagcag
    10021 aaaacttgga tgcgatgaag catcgacaag tccaatatct gattggatcg atgggtgggt
    10081 tatgtgtgtc ggcattcttg tcatttgtgc cgtttacgca gttgcagacc atttttctga
    10141 tcacttttct atatggaatc gcacaatttc tagttgctag aaattatgca gttgctaata
    10201 tttttcttaa tccgatggcg ttgatgctat ctacacttat tcgtggagca tacttgattt
    10261 ctttgatcga atatcgtttt ttagggatcg tcataggaag tttcatcggt ttaggtgttg
    10321 cgtgggtaat gacggttggt ttgcaacatt atttaacggt tgttcgtcaa aaattagaat
    10381 agaaggacaa atatatgtgc caaaagaggt tatgacaaga tttttgtcac aacctctaat
    10441 tcaaggtgtt caaaagccag attctcagca ttaagtcaaa ttcacacaaa aatgtggaaa
    10501 ggaactttca aaaaaatttt atatttattc tatcaaaaaa ttttgtttat gatggtaact
    10561 gaataaactt tcctgagatg gtcaatgctt tccttgaaag tcttacagat attctttctt
    10621 catagaaagt ttaagtttta tagttagtgt acaataaaag tatgtttata ccatatggtg
    10681 tccggataat cagtcattta aacaatgagg ttcaaagata tgaaaagggg aaatttgatg
    10741 aagaaaataa gcaatgaaat ccaaattctg gcagaaactt taactcttcc caaagtcgtt
    10801 atagagcaaa tgattgtaca agcaaaaaac atccttgatg aggtgtttga gcaatatagt
    10861 catgaactaa tggatcctaa taaagcaaag gcgactgcaa agaaaataga caaagtctat
    10921 gaagaggaac cttttgcagc attagctatt tatctatatg cagcttctca aagttggaaa
    10981 aaaatttatc aaccattgaa gattccaaga actatctatt tggcaacaat gaatgctttt
    11041 actagattta ttcatgaaca tttccaagta acaggaagct atcgctttga ccgtggtttt
    11101 tggatttggc attacactag tggcttgatc tttcggattg gtgaattgga gtacgaaatg
    11161 acatattttc cgaaagggta cacggatcta agattagaag gaaagcagtg cctttctctt
    11221 catattcctt ccgatgcaga tttatctcag aataagataa gagatagtta cgaaagtgcc
    11281 attcgttttt ttgaacttta ttttcctgat taccattacc aagtgatgta cacagatact
    11341 tggttactat cacccaatct tactaaatgg ttgaaaaaag agtcaaaaat ctgtcttttt
    11401 gcagccgatt atcgattatt atcagttgat gaacaggacg atagtggagt cccttggatc
    11461 tttggacgag tagatgccca aatacatgat tatccagaaa gtacgagttt acagcgacaa
    11521 gcaaaagagc aattgcttgc aggcgagcac attggctctg gcttaggtat tcttacttca
    11581 taagtttagt acttcgaaat agtttaaaag agcgtgagac aaaagtaaag aacacttttg
    11641 tctcacgctc taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct
    11701 gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc ttcttatttc ttggagttaa
    11761 acgcttctgt cccagcctct tttttagtgt tctatcgaac acacagtacg ttcaataggt
    11821 aaactagcta taacaataga tattaaagtt aacggacaag agggcctttt ttaggtagct
    11881 aaaacgtaat aatgaaaatt tatttccaga gataggagtc aatcagctag tctgatcgac
    11941 tgttttaaca agttgacttt cattttaact tttgatacca tttaaaacac aaatcgtaat
    12001 tgtttcgatt tgtgtttttt agcatgacta caatcaaaga agaaagtggg tcgtccatat
    12061 ggaaaaaaca gatatctcaa gtgcataccg ccgcttgaaa agcccaaata tcaaaacacg
    12121 aaaacgtgcg ctgaagatca tcaaagatgt gaaacggaat agtgggaaaa ggtaagaaga
    12181 aggaaactta attaaaaaaa tagccgaaaa acgtttttcg gctattttca ataatcggtt
    12241 tttgtgtaag gtttccgaaa gaactgctgt tgattttcca tttttaacat atgataaatt
    12301 ggtagaatct ttcttttcaa attgatacca ttccctaatt ccagttatat agttaactac
    12361 agaaccacaa agcacgtacg tttataaaag tattatctgt agttattttt caattccctt
    12421 tcaacctctt attttgacca gtagtattga tcactttacc ttttaacacc tttgttcgtt
    12481 tttgaaacat ttttagtcaa gaatggaaac gtaactacat aatcaaaggt tttgcgaaat
    12541 atttcatgaa atatttcatg tagaagcgag caatacggtt gcaaataacc attatcgttt
    12601 aataagcagt tacgaaagct cccaatatct ctaacgttca gctcactcca tttcttttct
    12661 tcaaaaaaat cagtgattcg tatctctttc tatttgtcga aaaagttaac tttaaaaagt
    12721 aacgccgaaa aaatatgcaa aaaaataaaa aagcagttac tattgctaga ataacatcaa
    12781 ggaataataa gttttggttt tcagataata gtcgataagt tgtgtattgg aattttagat
    12841 acagtaataa agtatttttc atgaaatttt gtgagaatca ctattacaag tatctttaat
    12901 aaaattaatg gttaaggagg aacttaaagt agaatgtaaa tgaatagttg tagtcagtcg
    12961 attgtgatac tagactcaaa gttgcaggaa taagtttttc aatttgtgat tggaagataa
    13021 aaaatgaaaa agaagggctt ttttttatac tttgttgatt gtagtgttta ttttccctct
    13081 gtttagccaa gtaacatttt ctatgactca accagtttca agtgaggaac atgataacct
    13141 ttttcgtgct acagaagagt ttaagaaata tcaggaaaat attgttccgg agactccatt
    13201 agctagtgag ccaattactg atattgatgg aaaccaaata ggatggtata tacagtacga
    13261 agttatggtg gtaaataatg actcaatagt aaaaaataca gtaggcttag agctaacacc
    13321 gattgaaaag gtgagttcat tgttaacgta tatttatact gatgaaagtg acgagactga
    13381 gatcatttta acggattact caaaaattgc tactgataaa aagtatatat tgtggatgta
    13441 gacactagtg aaatacttta tgatatagaa attgatgaaa atttactaga tgtagcacaa
    13501 gaaatcgatc atcaaaagca ggaagctatt cgatatgctg gtagaaaagg gaaaagaaaa
    13561 tatttattac gtgaatgtag caaaagttag agaagatgaa aatgagtgga aagaattcaa
    13621 gagtcgctac tcaattaact cgactccgac atttacagtt tacagagaag gatcgataga
    13681 aaaaactgtt ttttggacta aagaaagtgg aatgtcttta gctgaagtag aagagttttt
    13741 ggattatgtt agtatgcaac agtaagggag ttcttattat gaaaaatttt gacacatatt
    13801 atgttattgt tttttcgata tctttgctgt taatattact aactttaatg cagtataaag
    13861 agtatccaga gtattttatt ttaacttttg tatatttagt aagtagctac acacttctat
    13921 ataaattcag aagttcttct agagtttcta aagtttgttt cttaattagc ttattgattc
    13981 tataatattt ttgatttata taggaaatta aaaatattat agaatcaaca ctcagagaac
    14041 gggttggttt ttaatagatt taataagtac tgataatgaa atttctcctg gttgggaaaa
    14101 cgaagttggt taccctgtag aagaattttt tcaagattac tttgcggagt atgccgatag
    14161 gataatgaga gattacgtaa cagataaaac tcaagaagtc atcaatcaat cccagtcatt
    14221 aaatgaatta tctgataagg cgaaagaaga agcagttgcc attatttctt cagggattag
    14281 tgactatatt gatatgagtg gtgaaatcaa ttatggcgat aaagacaaaa ttgtacgcat
    14341 gataaaagct tttatcaaaa cttttgatgt gaaggcagat cttaagttta atgatactta
    14401 cctagtagga aaattaatta atatccttgg gacagagaaa gatacggaaa cgtttatttc
    14461 ctctttgtct atgttgttta cagataagca tttttcaact cagcttggtg gagaggaact
    14521 agtggaaatc ttttaaccat tacgcccagc attaataaaa gtatatcaaa aaagtactcc
    14581 tgatgatttt taagattggt ttgagaaata atgtgaaaac actattttta atgttaatta
    14641 tagatagaga tgaacgcgtc atatggttaa aagaatgaaa aagtcaaaga tacactgcta
    14701 tatttcacta aggatatagc agtgtatctt tcatgtcgtt tcactggcag ttttttataa
    14761 aaggaggtgt gcatttcagt tgcttttctt tggtagttag ttgaatcgtt taagaattaa
    14821 cggatgtggc agacaggtgc tctgtccaaa aaccagtaaa atctttgatg gttccatctt
    14881 tatttagctg tgcctgttca aatactgttt tgtttttacc gaatggatca attgcaataa
    14941 ttgttccgat ccccgcacgt ttggcagcaa gtaatcctga gtaagcatct tcgataacaa
    15001 gacaatcagc cggagctaag ctcagtttgt ctgccgcttt aagaaaaata tccggttgcg
    15061 gtttcccagg gaaagagccg tcgtcataga caatcttgtc ccagtcaaac cagcgggcta
    15121 aatcgaaaat atcaaaataa aaagcaacat tctctttgac agtagccgtg gcaatcgtta
    15181 atggaatctg ttggtcagtc agctggtcaa gagtcgcttc aagccctttt gtcaactgaa
    15241 gctcgttttc attttgcaag caaagcatac gataatagct ttctttttct tctgacaata
    15301 cggcgatctc agaggcggtc aaatgctctg aaatgaaatg tttcaaaatc tccgtattcg
    15361 tccgaccatg aatatttttt aaaatatcct cgtctgataa accatctttt gtatgatcat
    15421 gtatcatatg cagccaagca gcttcatgca gatgagagtc caaaaacatt gttccgttga
    15481 aatcaaatat aatccctttc atagttacgc tcctatagtg gtattttcgg ttgaatcagc
    15541 aatttgcaga tcgacatatt gtgcatagtg ctcttgaacc tcccgcgaga tctggcgatc
    15601 cgtaatgagc agatcgaaat catgcaaatt ataaaacaca taaaaatcat ctcgattgaa
    15661 ttttttatta tcaacgagta gatatttctt acgggaatta tttagagcga tctgctgtgc
    15721 ttctccttct tcggtactgt aagtagagat cgcttcgttg tgaacgccat ttgaactgat
    15781 aaatgctttt gtaaacttta gtcttcttaa attgtcgttg gtgatcggtc cgacaaatgc
    15841 gccggtattt ttccggaaat caccgccaat cagtagaatt tctggtccat ctgcttgagc
    15901 aagaatatca aaaactggat aattatttgt aatgatccga attttcttgt tcagtaaatg
    15961 cgtcgccaat agttcgattg ttgtacctgg tcctaagaaa atcgtttctc cctcgtgaat
    16021 caaggaggct gctgtggcag caatttcttt tttttcttga atctgcagag tggctttttc
    16081 agtgtgagaa agttcttgat ctaaagtgta ggaaagactt tgagcccctc catggacacg
    16141 cagcagcttg cccgattttt ctaattcatc taaatctctg cgaacggtca tatcagaaac
    16201 atctaattga ttgatgattt cattaacggt aaggattcct tttttattga ccatttctac
    16261 gatagtaagc aatctttctt ttttcaacat tgtcttcacc tctaatttaa ggataatcta
    16321 tcatataaac ggacaacagt caatctaatt cgaacaaaag taaacattta gggtggtttt
    16381 tatgtttgtt acaatagatt ttattgctgt tttctggttt ttatgattat ttgtgtttgt
    16441 ttttgttgac aaatgtttcg gatgcgttta caatgcaatc agttagaaag agcgaaaact
    16501 gattatagat ttgaggagag atgtacatgc ttaaattacc agaagatttt atttttggcg
    16561 gagccacagc agcctatcag gttgaaggag ccactaagga aggtggcaaa ggcgctgttg
    16621 cttgggatga ctttttagag gaacaagggc gattcagtcc cgatccggca agtgattttt
    16681 atcatcaata tgcaaaggac attgaattgt gtgaacgttt tggtgtaaat ggtctaagat
    16741 tatccattgc ttggagccgc atttttcctg atggagctgg gaaaccaaat ccagaaggaa
    16801 tcgcttttta tcatcgtgtg ttcgaagaat gtaagaaaag aaacgtaacg ccgtttgtga
    16861 cgttgcatca ttttgatacc cctaaacgtt tgtttgacca tggagatttt ttgaatcgag
    16921 aaacaataga agcatttgtt tcctatgcca ttttttgttt tcatgaattc aaagaagtca
    16981 aggtgtggag tacctttaat gaaatctatc cagttgcaac caatcaatat ttgttaggtg
    17041 ttttcccacc aggaatcaaa tatgatttta caaagatcgt cgcctgtctt cataatatga
    17101 tggttgctca tgcccgtgta gtgaactatt tcaaagaaaa cgaacttccg ggtgaaattg
    17161 gtgtagttca ctcattagag acgaaatatg cggcaacaga cgcgccggaa gataagcatg
    17221 ctgccttttt agacgatgca ttgtcgatcc gtttcttatt agatgctacc tatttaggct
    17281 attattcaac tgaaaccttg actgctcttg atgagatatg tgaagcaaat caggccagct
    17341 atcattttcc tgaagaagat ttcgttgaat tgaaaaaagc atccacgcgt aatgactact
    17401 tagggatcaa tcactaccaa tgccattttg tcaaagctta cgatggagaa aatgcaatcc
    17461 atcataacgg tacgggtgaa aaagggacct ctgtttataa agtgaaggga atcggagaac
    17521 gtatttataa agaaggaatt cctcgaactg attgggactg gctgatttat ccagaaggtc
    17581 tatatgactt gttgctgcga attaaaagcg attacccaca ttacaacaaa atctatatca
    17641 ctgagaacgg gatggggtat aaagatcagt ttgaagatgg cattattatg gatcagccga
    17701 gaatcgacta tttgagagtc tatttggaat ccttaagcaa ggcgataaca gccggggtaa
    17761 atgtgaaggg gtattttttg tggtcgctta tggatctttt ctcatggaca aatgggtata
    17821 acaaaaggta tggcttgttt tatgttgatt ttgaaaccca gaagcgttac ccaaaagaat
    17881 cagcttactg gtacaagttg gtcagtgaaa ccaaaacgat tatttaataa gtgataatgg
    17941 aggaagaaaa gatgaataaa gaggaattac aaatgcttgg ttttgagatc gttgcatatt
    18001 caggcgacgc tcgcagtaca ttattgaaat tattgaaaga agtaagacaa ggaaactttg
    18061 aacatgttga ttcggcgttg aaagaagccg acgaaaattt aacccttgca cataattcgc
    18121 aaacaaaaat tttagcggaa gaagcatctg ggaaagagat ggagatggga ttcattttta
    18181 tccatgggca ggatcattta atgaccactt tattgctgag agatctgatt caagacttta
    18241 ttgtactgta cagacagaat caaggatagg agaagaacgc tatggatgga atcattaaac
    18301 aaatcgaaaa agggaaacca ttttttgaaa aaatctcacg taatagttat ttaggagccg
    18361 ttcgtgacgg atttttaaca gcgatgccag ctattttatt ttcaagcatt ttcattttga
    18421 ttgctgcggt tcctgagatt tttggttacc aatggcctga atctgtctcc acatggctat
    18481 ggaaagtcta taattattcg atgggtgttg tagggttact cgtttcagca accactgctc
    18541 gctgcttggc tgattcgatg aatcgtcgct taccgaaaaa caaagtaatc aacaccacct
    18601 ctgtgatgct ggcctctgtg gtaagtttcc tgttgttaag cgtctctccg attgaaggtg
    18661 gattttcaac ggaatatatg gggacaaagg gactactggc ttcctttgtc gctgcatttt
    18721 taacagttaa tttatataag ttttgtgtgc tgaaagatat taccattaaa atgcctaaag
    18781 aagttcctgg aacgatttcg caaaccttcc gagatatttt tcctttctct ttttcagtat
    18841 ttgcggccgt tttaattgat ttaggcattc gacaaatctt ccaaatctca tttgcagaag
    18901 caatcattac ggtgctgcag ccgctattta cagcagctga cgggtactta ggaatcgcta
    18961 ttatctgggg agcgatggcg ctgttttggt ttgtcggtgt tcatggaccg tcgatcgtgg
    19021 agccggcaat tgcggcaatt atctatgcca atgtggaaac caatctgcaa ttgttcaatt
    19081 ccggtgaaca agcttctaaa gtactgacgg tgggtttagg gaattttgta ggaactatgg
    19141 gaggaactgg ggcaacattg gttgttcctt ttatcttcct actctttgct aagtctaagc
    19201 agttgaaggc agttgggaaa gcttccttta ttccagtaag tttcgctgtc aacgaaccat
    19261 tgctatttgc ggcacccatg attttgaacc cttatttttt tattccattt ttgttaacac
    19321 ctgttgtaaa cgtttgcctg ttcaagtttt ttgtcgatgt attgggcatg aacagcttta
    19381 tgtacgtttt gccttgggca actccagcac cgatcggttt ggttctagga acgggcatgg
    19441 gtatcttagc ttttgttcta gcagcggttc taatcgtcgt tgacttcctg atctatctac
    19501 cattttgcaa agcatacgat gctgtcttag tagagaaaga agcacaaaaa gcagaggtga
    19561 tcgcaaccga ggaaggtgct tctgccgatg tacgtgagga agtacctgtg gcagcgagtc
    19621 aagttatcag cgatgcaaat aaagaaatca atgtgttggt actttgtgct ggcgcaggga
    19681 caagtgcgat gttagctaat gctttaaaaa aaggtgcatc tgaatcgaaa gcaccgatca
    19741 gtgcaatggc gggtgcttat ggttctcatt acgaaatgct gcaggatttt gatatggtgg
    19801 tcttagcacc gcaagtcaat tcttactatg atgatatcaa agaagacacc gatcgcttag
    19861 gaatcaagct ggttgcgaca aaaggagcag aatatatcgg attaacgaga gatcctgaaa
    19921 aagctgttca gtttattctg aaccaatttc aataaaggag gagccataat gaaaatggga
    19981 atttggttgc gtaaagctgt tttatttgca gctgtgttag gggctttggg ggctgtttca
    20041 aaaactgttg aagcatctgc cagcgaattt attcgcggag cagatatttc gatcctgtca
    20101 gatatggaaa aatctggtgc aaaatattat gaagacgata tttcaaaaga tgccttagag
    20161 attttaaaaa ataatggtgt caactatgtt cgactgcgtt tgtggcagga tccttataat
    20221 agtaatggag aaagttatgg agcagggaca aatgatctgg aaacgacgat tgaccttgca
    20281 aaacgagcaa aaaatctggg acttaaggtg ctgctggatt ttcactacag tgatttttgg
    20341 gttgatcctg ggaaacagaa tcttcctaaa gcatggcaag gtctgacctt tgaagaaatg
    20401 aatacggcat tgtacgacta tacaaagaac gttctgtcgg aaatgaagca gctggacgtt
    20461 tatcctgata tggttcagat cggcaatgaa ctgaatagcg gaatgctctg gccttatggc
    20521 aagtcttggg gagaaggcgg gggagaattt gatcgattgg ctgctttttt gaagtctggt
    20581 atccaagctg ttcgagacac tcaacctaaa acaactccag taatgctcca cttagctgat
    20641 ggtggcgata caggagcatt tacttggtgg tttgatgaaa tcacgagtcg aggtgtgtca
    20701 tttgatttaa ttggagtgtc gtattatccg tattggcacg gttcaatgaa tgaacttcaa
    20761 accaatatga atgcgatcag ccaacggtat ggtaaagaag tgatcgtagt tgaaacagct
    20821 tatgggcata caacagcgaa tgccgatact atgccaaatg cttttggtga agccgaagca
    20881 gctgcggggg gatatgaacc aacaccgaca ggacaagcag agtatttgct ggatcttgcc
    20941 gatcggattc aggcagtacc gaataatcgc ggggcgggct tcttctattg ggaacctctt
    21001 tggtacaacg gaaatgtgag ttgggcaaca caagcaggta tgaattatct cagtgttcaa
    21061 tccactatgg gaaacgaatg ggacaaccaa gcgatgtttg attttgctgg aaacgccttg
    21121 ccaagcttgc gtgcgtttaa acaagcaggg gcgcaaataa atctagttaa aaatgcaagt
    21181 ttcgaaagcg atggaccaac gaccagtcct tccaattggc aaacctggtt tcaacaagga
    21241 accgctaagg gtacgatcaa aacggaagcg ggcacttttt ttggggacta caaactaact
    21301 ttttggcagg atcaagcata cgaggcatca gcttatcaaa atgttagtgg cataagcaaa
    21361 ggaacttaca cattgagtgc ctatgtaatg tctgggggag agtttgatac taatcagtta
    21421 tatatcaaaa attatggtgg agcggaacta aatactgcgg ttgttaaaac aagtgaatgg
    21481 acaaaaatta caatcaaaga cattccaatt acaacaggtg tttgtgaaat tggggtttat
    21541 agcaaggctt caggaaatgc ttggacgaac gttgattttg tttcattggt gaaacaatag
    21601 actgcggatc gtttcgattc aagatcggag gttcccttca gccacatcgt tagcgtagaa
    21661 ggcaggaatc gaacctgcac ctcgcctttg ccaaaaataa ccagaaacga tttccagtca
    21721 tttttaccaa aacgagtaaa gcaccagact tcatctacac tttgtattat gctaaagaga
    21781 gttcaacgat ttcgtaagaa gcaatgaaaa aattgcaaaa agttgtgcag ataacataaa
    21841 gtttgtttaa gaaagggtaa ccttagaatt ccgtcgtgaa tatgatttga gcacttagat
    21901 ggatagaaaa aagttctcgg aaaacagttc gtttttcgag aacttctttt tatgataaga
    21961 atgaaacaaa ttaacttata ttatagtctc ttttggtagg gaaccatctt atttttaatg
    22021 aaaatcaagc attttagcta ttgaagaatg ttgatttata gggattcccc ctccatatta
    22081 tgttggctct ttattcccac tccactgttg ctggtggttt agaagtgatg tcatacacga
    22141 tacggttgac gtgggctact tcgtttacga tacgtacaga gattttttgc aagacttccc
    22201 atgggatacg ggcaaagtca gcggtcattc cgtcgattga agttacagca cggataccga
    22261 ctgtgtagtc gtaagtacgt ccgtcaccca taacaccgac agagcggatc cctggaagaa
    22321 cagtgaaata ttgccagata tcacggtcaa gtccagcagc tgcgatttct tcacgcaaga
    22381 tagcatcaga ttcgcgtacg atttctagct tgtcttcagt gatttcgcca agaacacgaa
    22441 taccaagacc aggaccaggg aatggctgac gccaaacgat tgaatcaggc atgcctaact
    22501 gagtccctaa ttcacggact tcgtctttga ataatgtgtt taatggttcg atcaattcaa
    22561 attgcatatc ttctggcagt ccacctacat tatggtgaga tttgatcgtc tgtgctgttt
    22621 ctgtaccaga ttcgataacg tcagtataca acgttccttg tgctaggaaa gagacgcctt
    22681 cttcaccagc aagttttgtt gcttcgtcat caaaaacata tacgaattca ttcccaatga
    22741 ttttacgttt ttgttctggg tcagatacac ctttcaactt gtctaagaag cgatctcttg
    22801 cattgacttt gatgatgttc aagccgaatt ttccgcctag actttccatt acttgttccg
    22861 cttcgccttt gcgtagcaga ccgtggtcaa caaagataga tgtcaattgg tcaccgatcg
    22921 ctttttgtaa aagaacccca acgacactag aatcaacgcc acctgaaaga ccaagcaaga
    22981 cttttttgtc accaacttgt tcacggattt tttctacttg catatcgata aagttatcca
    23041 ttgtccagtt accttcacag tgacaaacat caaaggcaaa gtggcgcagc aattcgattc
    23101 cgtattcgga atgacgtact tctgcatgga attggatacc ataaaaatta cgtgttgtgt
    23161 cttggataga agcaatcgga caatctttgc ttgttccgac tgtttcaaag ccttcaggaa
    23221 ctttagttac caagtcacca tggctcatcc atactgtttg ttgttttggt gtttctttaa
    23281 acaagacagc ttgatcgctt gttacttcta acaaggcttt cccatattcg cggtttttag
    23341 caggctctac ggaaccgcca agtttgtacg tgatcaattg cataccatag caaatcccta
    23401 atacaggaat acctaaattg aagatctcag gatcgatccc aaatgcattc tcatcatata
    23461 cactgtttgg tccgccagaa agaatgattc cctttggatt catcgctttt acttcttctg
    23521 cagtaatgcg gtggctcaat aattcggaaa agactccaag atcacgaata cgtcttgtga
    23581 tcaattggtt atactgacta ccgtagtcta atacgataat cttttcaacg ttcgtcatat
    23641 cggcaacgtt tgtcaaattt attaccccta tccctttaaa ttttatctca aaatagtcaa
    23701 actattttaa aatgctaata ttttcgcatg aaaatcgaat cgatgatatg attttcagtt
    23761 ttatggagaa ttatatcggc tcttcctcgt gtaggcaaga tatattcctg taaatttttc
    23821 aaattcacat ttttccatac atcttttgcc attttaaagg cttcttcacg atctccgata
    23881 gcaaaacgat aataataatt ctctggattt tggaaagccg tatccagtaa cgcaccaaaa
    23941 cgatccaaat accatttttc gatcagcacg ggatcggcat cgacatagac agaaaaatca
    24001 aaaaagtcgc tgacgtagat ttgctgattc gcaggtaatt gaagcgtatt gatcccctca
    24061 acgatcaaga tatcaggctg ctggatcaat tcgtacttgt ctggaatgat atcatacaca
    24121 tcatgtgagt aaacaggtac ttttatctct tcctgaccag attttacacg gttcaagaaa
    24181 tcgatcagca tctccatatc ataactttca ggaaatcctt tacggtccat gattccacgc
    24241 tcttttaata tacgatttgg ataaagaaat ccgtcagtcg tgatcagctg aacgtttctt
    24301 cgtttgaagg tacgagacaa aatcgtctgc agcagtctgg ctgttgtact ttttccaacg
    24361 gctacacttc cggcgatccc gataataaaa ggcggtactt ctacgtattt atgaagaaat
    24421 aaacctttac tcaagaacaa agattcaaat tctttcatat aaagataaat cagatgagca
    24481 agaggtacat agatatctcg aacatcctgc atggaaatgc ggtcattcaa acttttgata
    24541 ttatctagtt catcttgtgt caaaggtgct tgcccattac gataaaattc tcgccattct
    24601 tctcttgcca cacggtagta gttcattgga tcattcatat tagcctcctg cgttctccca
    24661 tcaatggaac tattttaaca caaaataaac gcaaacccca ccacttaaga gagattgccg
    24721 atctttttgt ataatcttta taatagaaag aaacggagtc ttattcttga cttgcgcacg
    24781 gatacataaa aggatagact gttgtcatga aagaaatgag aaggatggaa gacatgacaa
    24841 atcattacta ttctgaacgc ccagatacgg cgcacgattt tgaacaatgg gcttttgagt
    24901 taaaaggcag aacctttcaa tttgtaacag attctggtgt attttcacgt gaaacagtgg
    24961 attatggttc acgtgtcctg attgatgctt tcgattggga agagttacct gatgaaggaa
    25021 agatcttaga tgtgggatgc gggtatggac cgattgggtt agctttggca tttgcgacac
    25081 agcggtttgt tgaaatggtc gacattaatg aacgagcggt aagtctagcg caaggaaacg
    25141 caaagcgtaa ccaaatcgaa catgttgata tccatcaatc gaatatctat gaagcagttc
    25201 atgaagaaac ctatgcagct atcgtaagca atccgccgat ccgtgctgga aaaaaagttg
    25261 tacatgaaat tttgacaggc gcatatcctc ggttgaagaa gggcggtaca ctgacaattg
    25321 tgatccagaa gaaacaagga gcgccgagtg cgcaaaagaa aatggaagaa acatttggta
    25381 atgcagaaat cgtcacaaaa gataaaggat attatatcat caaaagtgtg aaagaataac
    25441 tagctgaaaa atagtggagc gacgaaagag tggaactggg cgtgcatgta cgggtacgta
    25501 tcagttcttt ttttgttgag aagtttttaa aaacaaagaa ctattttttc ttcaaaaaga
    25561 aatagtagga acagagtcgt tgttttttac gataaaataa ataagataca gaacttgtat
    25621 agaaggctgc caagtcggta gataaaggag gtttttatca aaaaacatac tttccttttt
    25681 tctctattct tttcttgggg gttttggtac actgataggg aatttagata gaagggagcc
    25741 aataggatga agctgaccat cagagatatc gcagaaatgg caggcgtatc agtaacaact
    25801 gtctcacaga ttttgaataa caaaggaagc agatttagtg ataagacaag aaaaaaagtg
    25861 ttgtcgatcg tgaatgaaca tcattataag cccgattatt tcgcttcaaa tgtgatcaat
    25921 cgacattcaa agaccatcgg aatgatcgtc cctgatgtca ctgatttctt cttctccaaa
    25981 ctgatcgaag gtgtagagag ctatttgaat cctttgggct atgtgatcat gttatgcaat
    26041 tctcgtcata gccaagaaaa tgagatcaag tatttacaag agctttccca ccgctcagtg
    26101 gacggaatct tgctggccac accaaatatt ttacctgaga aatatgcatt agacagcggt
    26161 ttttatcaaa aaatgccaat cattttaatc gatcgcggat tgaatcgtcg ggataacgga
    26221 cggcttatcg taaaggaata tgagggcgcc tatcaagctg tttcgctgtt tattgaaaat
    26281 ggccatacga agatcggcat gctgaaggaa accactggat actatcaatt agaagaacgt
    26341 ttcaacggat atcgtcatgc gctgaaagat catggcctgc cttttaatgg gaaatttgtt
    26401 gaacatggag agttgactgt ccaaggtgga tatactgcat caaaaaaatt actcaaacat
    26461 aaagatatca cggctatttt ttgcgggaat gatgccatgg cgatcggatg ctatcaggcg
    26521 atcgatgaat tgggcaaaaa aatcccagaa gatatctcag tcataggatt cgatggatta
    26581 aagctgtcag aatacatgat tccgcaattg acgacagtgc agcagccaag ttttgatatt
    26641 ggattttatg ctgcacgttt cctgatcgat acgatcgaat ttcctcaaag aaaagttccg
    26701 aacaaagtgt tcgaaacaaa attaattatt cgtgagagcg taaaagtatt gacaaagggc
    26761 taactcggcg gtatattgct tatgtaagca tttacaaagg ctagttagtg ctttttttat
    26821 ttttagtaaa ccagtttact aaagtggttt acctttccta aaaataatgt ataataccga
    26881 atgaagacaa ggaggatcag gcaatgcgaa tggtggatct gattgaaaaa aagagggatg
    26941 gaaacgagct gtcgaaagag gaaatcgagt acatcgtcac gaactatacg aatggaaaga
    27001 ttccagatta tcaagtcagt gcattattga tggcaatttt ttatcaagac atgacaaatg
    27061 aagaaatcac gaacttaaca ttggcgattg ctaattctgg tgatgtcatc gatttgagtt
    27121 ctcttgaagg aataaaggta gataagcatt cgacaggtgg cgttggtgac acgaccacat
    27181 tgattttagc ccccctagta gctagtgtcg gagtgacagt ggctaaaatg tctggacgcg
    27241 gtctaggtta cacaggtggt acactagaca agctagaagc aattcccgga tttcagatcg
    27301 agctttcgga tgaagaattt gtacgcattg ttaatgaaag taaagtagcg gtaattggtc
    27361 aatcaggaaa cttggcaccg gcggacaaaa aattgtatgc cttgcgtgac gtcacagcga
    27421 ccgttgattc tcttccgctg attgccagtt cgattatgag taaaaaaatc gcggcaggag
    27481 cagatgcaat cgttttggat gtgaccactg gagacggtgc tttcatgaag aacatcgaag
    27541 atgcaagacg tctggcaaag accatgacga gcattgggaa actggctaac cgagaaacag
    27601 tggcggttat ctcagatatg tcagaacctc ttggcgaagc tatcggaaat agtctagaag
    27661 ttgtcgaggc aatcgaaaca ttacaaggaa atggtccaga agatctagta gaaatgtgct
    27721 acgccttagg aagccaaatg gttgttcttg ctggcaaagc taagacaacc gatgaagcaa
    27781 gaacgttgct tcaagaagct ctggaatcag gtaaagcgtt agcaaaattc aaagaaatga
    27841 tccaaaatca agggggagac ccagcgatcg tggaacatcc agaacgaatt ttgactgcac
    27901 gctacacaat ggagcttcct gctaaacaaa gcggagttgt gtcaaaaatc gtggcaaatg
    27961 aattaggaat tgctgcaatg atgctaggtg ctgggcgtaa aaccaaagaa gaagatattg
    28021 atcacgctgt tggattgaaa ctgcataaaa aaattggtga cactgtgaca aaaggtgagt
    28081 cactattgac gatctatagt aacgacgaag aaatcacttc tgtcattgaa ttattatata
    28141 aaaatattga aattggtgaa tcggcaatga agccaacgtt gattcatgac atcatcacag
    28201 aataaaggaa aaaagcggac tttaccgttt taaagaaaag gggaaaagaa atggaactaa
    28261 accgaatgat cgaccatact attttgaaag cagacgcatc taaagaagct gtgatgcaaa
    28321 ttatcgaaga agcaaaaaaa taccattttt attctgtctg catcaatcca gcatgggttg
    28381 cgctagcgaa agaacaatta caaggtgagc cagtagccgt atgtacagta atcgggtttc
    28441 ctctaggagc aaacacaccc gagacaaaag catttgaaac aacagatgcc atcaataacg
    28501 gtgcagatga agtcgatatg gtcatcaata tcggtgcttt gaaatcaaag gatgatcaac
    28561 aagtccaaaa agatatcgaa gcagttgttg aagcagcaaa agatcgtgcg cttgtcaaag
    28621 ttattgttga aacatcgtta ttagatagag aagaaatcat ccgagcttgc gaaatagcaa
    28681 aagctgcagg cgctgatttt gtcaaaacat caactggttt ctcaacaggc ggagcaaagg
    28741 tagaggatgt ccgtttgatg cgtgaaactg taggaccaga aatgggtgtc aaagcttccg
    28801 gcggtatcca taacgaagaa gaagcaatgg caatgatcga agctggtgct acaagaatcg
    28861 gaacaagcgc aggagtagca atcgtatctg gttcaactgg tgaaggctat taattttttc
    28921 aaaggagaag ctcatggcag aactcaaaca agaatggatc aacacagcta ttgaagcgtt
    28981 agataaagcc tatgttcctt attcccattt tccggtaggc gcttgtctcg taacggaatc
    29041 aggcaagatt tatcaaggca tcaacattga gaatgcatca tttggattaa cgaattgtgc
    29101 cgagcgcaca gcttttttca aagcagtatc tgaaggagaa aaaacattta cgcatctagt
    29161 cgtagcgggg cacacccccg atcctatctc tccttgtggt gcatgtcgac aagtaatggc
    29221 agaattctgt tcccctgata tgccggttac gctagtagga gacaatggtg tgacgaaggc
    29281 aacgaccgtt cgtgcactgc tgccttatgc atttactgaa aaagatctat aactcatgtt
    29341 cattcagtca gctgactgtt gaaaaataaa acaattattt taggaggctt tacagaaatg
    29401 aaaaaagcaa aattatttgg tttaggtgct gtcgcactag cagcagggtt atttttaggt
    29461 gcatgcggca acaacggctc tactgattca tccggtggca aaagctcatc agacacaaca
    29521 actgcagcat tgatcacaga cacaggtggt gtagacgacc gttcattcaa ccagtcagct
    29581 tgggaaggac tagaaaaatg ggggaaagat caaggacttt cacgaggaaa tgatggcttc
    29641 caatatttcc aatcttcaaa tgaatcagat tacatcccaa atattgacca agcattgaat
    29701 gctggattca aaacgatttt tggtattggt tataaactaa aaccagcgat ccaagaacaa
    29761 gcaacaaaca atacaggtac gaactttgtt attatcgatg atgtcatcga tggattagat
    29821 aacgttgtat ctgcaacttt caaagacaac gaagcttctt atcttgcagg tgttgcagca
    29881 gcttatacga ctgaaacaaa cgtagtcggc tttatcggcg gtgtaaaagg tgaagtaatc
    29941 gatcgttttg atgctggatt caaagccggt gttgatgcag gtgcgaaaga attaggaaaa
    30001 gaaatcaaag tattgaatca atatgctggt gacttctctg ctccagataa aggacgttca
    30061 attgctcaag gtatgtatgc acaaaacgca gatatcatct tccatgcttc aggcggtaca
    30121 ggtaacggtg tattccaaga agcaaaatca ttgaacgaat caggcgacaa aaaagtttgg
    30181 gttatcggtg ttgaccgcga ccaatcagac gaaggcgaat acacattaaa cggcgaaaag
    30241 aaaaacttca cattgacatc tacactgaaa gcagttggaa cagtagtaga agatttggca
    30301 caaaaatcag cggacggtaa attccctggc ggcgaacaca ctgtttatgg attgaaagaa
    30361 gacggtgttg gtctaacaga aggacaactt tctgacgaag cgaaaaaagc agttgatgaa
    30421 gcaaaagaaa aaatcatttc aggtgacgta aaagttccag aaactcctga agaaaactaa
    30481 aaccatttct atagccttgt tagacacatg tttgacaagg ctatggtttg attagaacaa
    30541 gaattgttca tttatccaag ctgccttgtg acagtttgga taagggaaca actccctgaa
    30601 gtaatgtttg tttcaaaaga agaacgctca ctaagcgtga atcttagtca atcgttgccc
    30661 tagatagaaa tcgttaggat gtgaaaaaaa ggtggttcaa gaaaatattg tcatagaaat
    30721 gcagaatatt accaaagaat ttggaacatt caaggcaaat gacaacatca atttacaagt
    30781 aaaggcagga gaaattcatg cgttgcttgg agaaaacggt gctggcaaat ctacattgat
    30841 gaacgtgctt tccggattat tagagccgac atcagggaaa attttgatgc gtgggaaaga
    30901 agtaaagatc acaagtccga ccaaagccaa ccaattaggg attgggatgg tccatcagca
    30961 ctttatgctt gttgatgcct ttactgtaac agaaaacatc gtgttgggaa gcgagcctag
    31021 tcgcgcaggg atgcttgacc ataaaaaagc acgaaaagag atccaaaaag tttctgaaca
    31081 gtatggattg tcagtcaacc cagatgctta cgttcgtgat atttcagttg gaatggaaca
    31141 acgggtagaa attttaaaaa cactttaccg aggagcagat gtactgattt ttgatgagcc
    31201 gacagctgta ttgacccctc aagaaattga tgaattaatc gtgatcatga aggaattagt
    31261 caaagaaggc aagtcgatca ttttgattac gcataagtta gatgaaatca aagcagtagc
    31321 tgaccgctgt acagttatcc gccgtggaaa aggaatcggt acagtcaacg ttaaagacgt
    31381 aacctcacaa caattagccg atatgatggt tggaagaacg gtttcattca aaacgatgaa
    31441 aaaagaagcg aagcctcaag aagtcgtttt gtctattgaa aatctagtgg tgaaagaaaa
    31501 tcgtggatta gaagccgtga aaaacttgaa cttagaggtt cgtgctggcg aagtacttgg
    31561 tatcgctgga atcgatggaa acgggcagtc ggagttgatc caagctttga ctggtttgcg
    31621 aaaggcagaa agcggacata tcaagctaaa aggggaagac atcactaata aaaaaccccg
    31681 aaaaatcact gaacatggtg taggacacgt gccagaagac cgtcataaat acgggttagt
    31741 cctagatatg acattatctg aaaatatcgc actacaaacc tatcatcaaa aaccttacag
    31801 taaaaacggc atgctgaatt attcagtaat gaatgaacat gccagagaat tgatcgaaga
    31861 atatgatgtt cgaacaacga atgaacttgt tcccgcaaaa gctttatcag gcggaaatca
    31921 gcaaaaagca attatcgctc gtgaagtcga ccgagatcct gatctgttga ttgttgcaaa
    31981 tccaactcgt ggactggatg taggtgcgat cgaatttatt cataaacgtc tgatcgaaca
    32041 aagggacaaa tacaaagcag tgttactgat cagtttcgaa ttagaagaaa ttttaaatgt
    32101 ttcggatcgt attgctgtta tccatgaagg agaaattgtc ggtatcgttg atccgaaaga
    32161 aacatctgaa aatgaattag gtctattgat ggcgggctat accttagaag aagcacgaaa
    32221 agagttagaa aagggggctg gcgcacatga atgatcgcag cgaaaaattc cgtaatttat
    32281 tagtgcctgt actttcagtt gttttaggat tgatcttagg cgcgatattg atggctgctt
    32341 ttggttttca gcccatccaa ggttattctt ctatgctaga tgcagccctt ggttcgcaaa
    32401 gagggatagg tgaaacgtta aggcaagcaa cgccgttgat ttttacagca ttaggttttt
    32461 ctgtagctaa ctcagctggg tttttcaata ttgggctttc tggacaagca ctttgcgggt
    32521 ggatcgtcag tgtctgggtg gcacttgcgt ttccagatct gccaaaagtt gttttgcttc
    32581 cgttatgcgt gatcttaggt gccttagctg gtgcgatggc agcggctgtt ccaggtttac
    32641 ttcgagcatt ttttggaaca agtgaagtga tcgtaacaat catgatgaac tatatcttat
    32701 tatacgtaag cacatttgct ttacatgaag tgatgcctga aagctatcgc tcaagtttgg
    32761 actcttccaa tatgatcagt caaaatgcct cactgcggtt accatggtta acacaagctt
    32821 ttggcggctc tcgcgtaaac tcaggattat ttttagcaat catcgcgctt attttcgttt
    32881 ggctgctgat gaagaaaacg actttaggat ttgaaatccg ttctgttgga ttgaatcctt
    32941 ttgcttctga gtatgcagga atgagtagta aacgtacgat cgttttgtcg atggtcattt
    33001 ctggcgcttt ggcagggctt ggcggtgtgg ttgaaggatt aggaacgtat caaaacttct
    33061 ttgttcaaac gacttcgcta tctattggat ttgacggaat ggctgtttcc ttgcttggtt
    33121 ctggatcagc aatcggtatt cttttatcag cgatcttgtt cagtattttg aagatcggtg
    33181 gacttggcat gcagacagga gcaggtgttc cgtttgagat cgtcaatgta tcgattgctt
    33241 tgattatctt ctttgtcggg atcaacttcc tgattcgttt cgtcatggct aaagtcttcc
    33301 aagggaaaaa acaagaagag atcgttgcga caatagaatc gaaaccaacg aatcaaaacc
    33361 atgaaggggg cgaactataa tgtcgactat cgaattaatt gccttgatcc ttacttctac
    33421 actggtttat tcaacaccgc taattctaac gtcactaggc ggaacttttt ctgaacgaag
    33481 cgggatcgta aacgtagggc tagagggaat catggtcatg ggtgctttta gttccatcgt
    33541 tttcaacttg acgatgagtc catcacttgg ttctatgaca ccatggatcg ctattttagt
    33601 gggtggcgtc gtaggtatcc tattttctct gattcacgca gtagcaacag tcagtttgcg
    33661 tgctgaccat attatcagcg ggacagtaat taatctgatg gcacctgctt taggtgtttt
    33721 cttgatcaaa gcatggtatg gtaaaggtca aactgataac atcacgcaga atctggggta
    33781 tttttctttc ccaggattag cagatattcc ggtcattgga ccaattttct ttaaaaatac
    33841 attcttacct gcttattgtg ctattttgat tgctgtccta gcttgggtga tcatcttcaa
    33901 gacgaaattc ggcttgcgcc tccgttcagt tggtgaaaat cctcaagctg ccgacacatt
    33961 aggtatcaat gtatatgcta tgcgttattc aggcgtactg atttctggct tgttaggcgg
    34021 tatgggcggt gctgtttttg ctcagtcgat ttccggtaac ttttcagctg gtacgatcgt
    34081 tggacaaggg ttcatctcca tggctgctat gatctttggt aagtggaatc ctttaggggc
    34141 aatgggtgcc tcattgttct ttggttttgc tcaaagcttg agtatcattg gcgcacaatt
    34201 accagttatt tcttcgattc cgccagtatt gctgcaaatt gctccatatt tgctgacaat
    34261 cattgtgctg gttgttttct tagggaaagc ttctggtcca aaagcaaacg ggaagaacta
    34321 tatcaaatca aaataatctt ttagcgaata aaaagggtgg gttacgggaa gtgtttttcg
    34381 tctcgccctt tttgttttaa aggcaataaa aataaaaaga aagaaggaat aatcatgttt
    34441 aaacgcgtac atctgatcgt catggattca gtaggaattg gagaagcacc agatgccgat
    34501 aagttcggtg atgttggttc tgatacatta ggacacattg cgaaagaagc aggattgaac
    34561 gttccacatt tagagcagct tggtttagga acgatccgtc ctcttgaagg tgtggaatca
    34621 gtgaaagatc atcaaggcta tgcgacgaaa ttagaagaag tttctgtagg gaaagatacg
    34681 atgacaggcc actgggaaat catgggattg aatatcaaaa ctccatttcg tgtgtttcct
    34741 aatggattcc ctgatgatct gatcaaacaa atcgaagaat tttctggccg taaagtcgta
    34801 tgtaacaaac cttatagcgg aacagctgtt attgacgatt acggtgaaca ccaaatgaaa
    34861 acaggcgatt taattgtata tacttctgcc gatcctgtcc ttcagatcgc ggcacatgaa
    34921 gaaatcattc ctttagaaga attatatcgg atttgccaat tcgttcgtga tataacaaaa
    34981 gatgatccgt atatgattgg ccgaatcatt gcacgtccgt acgtgggtga accaggagac
    35041 ttcacacgaa caagcaatcg ccatgattat gcgttgaatc cattcggaca cacagtcctt
    35101 gattctttga aagaagcagg aaaggacgtg attgctgttg gaaagatcaa cgatattttc
    35161 aacggacaag gaatcactga atctgtccgc acgaaaagca atatggatgg cgttgaccag
    35221 ctgctgaaag ttatgaaaaa cgatttttca ggaatcagct ttaccaattt agtagatttt
    35281 gatgcattat acggtcatcg tcgggatgta gtcggctatg cgcatgcgat tgaagaattt
    35341 gacttacgaa ttccagaatt gcttgaagcg atggaagaag acgacttgct aatgattaca
    35401 gccgatcacg gaaacgatcc gacgtttact ggtaccgatc atacgcgaga atatgtaccg
    35461 ttattggttt acagtaaaaa aatgaaagaa caaggggcat tgcctcaagg atattattca
    35521 gatatttcag caacgattgc tgagaacttt agtgtttctt cgactgaaaa tggcgaaagc
    35581 ttcttgaaat tattaaaata atctttttaa ataggagaaa gcaaatgacc aagttaagcg
    35641 agatgttaca agaaacgacc cagtttatcc ttgataaagg agtaaaagaa attgattttg
    35701 gtttgattct aggttcagga ttaggtgaat tagcagatga aatcgaagat gctgtaatga
    35761 tttcttatga tgagatcccg ttctttcctg tttcgacagt agttgggcat gcaggaaagc
    35821 tagtttatgg gtctctggca ggcaagaaag tgttagccat gcaagggaga ttccactact
    35881 atgaaggtca ttccatgcaa acagtgacct atccagtacg tgtgatggcc ggactagggg
    35941 cccattcagt cattgtgact aatgcatgtg gtggtgtcaa cgaatcattt gtaccaggag
    36001 atttgatgct gattaccgat catatcaatt ttactggtga aaatcctttg atcggtccga
    36061 atgaagatgc aatgggccct agatttccag atatgagtca agcttatacg cctgactatc
    36121 aagcaacagc aaaagcagca gcagaaaaac taggtttgga tcttaaagaa ggcgtatata
    36181 tgggctattc tggaccaact tatgaaacgc ctgcggagat ccgaatgtct cggacaatag
    36241 gagccgatgc agtagggatg tcgactgttc cagaagtcat cgtagcagct cacagcggat
    36301 taaaagtgtt aggaatctct tgtattacca acttagcagc aggtatgcaa gcgaatctga
    36361 gccatgaaga agttgtagaa acaactcaga gagtcaaaca atcttttaaa gcattggtta
    36421 aagaaacatt gacattattg taataggagg aacaataaaa tgagtgtgca tattgaagcg
    36481 aaagaaggcg agatcgccga taagatttta cttccgggag atcctttgcg agctaaatat
    36541 attgcggaga cttttttgga agatccagtt tgttacaatc aggtacgcgg tatgttaggc
    36601 tttaccggca aatataaagg agaacgtatt tctgttcaag gaacaggaat gggaatgcca
    36661 tcagccacca tctatgccca tgaattgatc cagtcgtatg gtgtaaagaa attgattcga
    36721 gtaggtacat gcggtgcatt gtcaaaagac gtccatgttc gtgatttagt actagcacaa
    36781 ggtgcagcga ccagttcttc gatgatcgaa aaaaatttcc aagcctttca ttttcctcca
    36841 atcagtgatt tcaatctatt actgaaagca tatgagatag ctaaagaaaa gggctatacg
    36901 gttcatgtag gaaatgttct ttcggaagat tctttctaca aagatgattt gacagagacg
    36961 ttccaattag ctgaacttgg cgttttagga gtggaaatgg aagcagcggc tctttattac
    37021 ttaggcgcga aataccatgt gcagacatta agtttgatga ccgttagcga ccatttgatc
    37081 accggtgaag aaacaacagc tgcagaacgt caatctacgt tcaatgaaat gatcgaagta
    37141 ggattggaaa cagcgatagc agaataagaa ggaaatgaaa aagctgccct gcattaagta
    37201 ataagaacat ccaagcaaaa atagctcctc atgtttttga cttggctgga cttattattg
    37261 caaatgtaag cttttgaaca ccgtttacca agaggttgtg aatctgccgt ttaactctga
    37321 gtcacaagga acaatttcaa aaaacagctt ttcatgtttg atgaatattg tgacttgtga
    37381 ccgagaagtt ggcaggagaa aaccgtttat acgaaaaaag ggactgtgac aaatcgtgtc
    37441 acagtccctt ttttatagtg ttttttatag gtagtatttt ttagcaactg ttaagtcatc
    37501 atttaattca taaacaagtg gttgacctgt tggaatttca agatccatga tgtcttcgtc
    37561 agagatacct tcgatatgtt tagctagggc gcgtaatgag ttaccatgtg cagctactaa
    37621 aacagttttg ttgtctagta atgctggagc aatttcatct tgccagaatg gcaaagcgcg
    37681 ttctaatgtc actttcaagt tttctccacc aggaatatcg cgtttgtcta acattgcgta
    37741 acgacggtca tttgctgcag aaccttcgtc agtttcttcc attaatggag gaagagtatc
    37801 gtaagaacga cgccagatgt gcacttgttc gtcgccgtat ttttcagctg tttctttttt
    37861 gttcaaacct tgtaatttac catagtgacg ttcgtttaag cgccatgatt tgatttgtgg
    37921 tacccaaagt tgatctgaat attctaagat caagttacat gttttgattg cacgagttaa
    37981 aacagaagtg taagcaacat caaattcgat tccagcttct ttgattttgc gtccgccttc
    38041 ttttgcttct tcgattcctt ctggtgctag atttacatca gcccaaccag taaattggtt
    38101 caatgcattc cattcactaa gaccgtgacg agaaaaaact aattttggca tgtttagctc
    38161 tccttttaag ataggtttat atttctgaaa ttttcagttc tgttttattg tacaattttt
    38221 tggaagagat ttccatcgaa aaattagata agattctatg agaaaacaag tagagaatat
    38281 aaaaaaacga acagtgccca cgcaactgtt cgtttttagg tattagatgc ataattgctc
    38341 gatttctttt tcaggtaaat tgatatattt ataacatgtt ccaactgaaa caccgcattt
    38401 tgaagaaata aattggatcg tttctttttt ttcataatag agacgtcgga tttttttgat
    38461 cgtttttgca tcgatttttg gtcttccgcc gatttttcct tttttacgag cttcatctaa
    38521 tccaactaat gtacgttctt tgatcaatgc gcactccatg tttgctaggt tttccattaa
    38581 ttggaaatag atttttccca tttcgcgcgt atcgattcct tcatcaagac taatcaagtg
    38641 cagcccgcga tctttgaata attgcgtgag ttctgttaat tgccgcgtag atttgcctaa
    38701 tcgatgaagt tggcaaatga ctagtgcatc gccttgttgc atgtcagcga tcactgactc
    38761 caaatgaaca gcagtagaat cattttgttc cacttcatat gtctcatgga aaatttcttg
    38821 acaaccaaag tcagataatt gatcaacctg agttgttaaa tcaacgtcat ttatggttgt
    38881 gcgtgcataa ccaataattt tcatggtgat tcctttctag cagtgatttt ctaactggat
    38941 ataacagtaa aaatgtagca taaagtacgt caaaaagata gttttttcgc aaattttccg
    39001 ctgtcttttt ttattggatg atcaagcagt ttttctttat ttacgacgga ttgcgtcaag
    39061 aatctcaacg cctttttctg agccgacaac tactttagaa accatgttta ggaaaagccc
    39121 atgttctact accccaacaa gttcatctaa ataggtagct aagctttctg gctgttcgat
    39181 gacttctaga tgcagatcaa tgatgtaatg accaccatct gtggttaaga cttctccgtt
    39241 ttcgtctgta cgcaagacgg gttggaatct tttttcttca aaaatatgaa gtagctgttg
    39301 ggagccataa gggatcactt caactgggag tgggaacttc ccaagtttat cgactaattt
    39361 agaagaatca acgatccaaa tcgtttcttt tgagtacgta gcgactattt tctcgaaaag
    39421 caaagcagcg ccgccgcctt tgatcccttg aaaatcagca ctgatttcat cagcaccgtc
    39481 tattgtcaaa tcgactacgg ggacttcatc aatactcttt aacggtattc ctaatttttc
    39541 cgcttgttct tttgtcgcat ttgaagtagt aactcctgtg atacgtaaac cttcttcacg
    39601 catacggcga ccaatctctt ctaccatata ataggcggtt gaaccggtcc cgagacctac
    39661 gatcatcccg tcctttacga atttcgcggc ttcaatgcca gctaattgtt ttaaattcat
    39721 ctttgcgtcc accttttttt agtttgagca tcgtgctttc ctcactattt tagcgtgatt
    39781 tacggtaaaa agataccatt cttttttatt ccaataaagc tttttcaaaa aaaattgcat
    39841 ctcttgttga catgtttttt cattcgtgat attctaacaa aggtgcttta tctacaggtt
    39901 tctgtgaaga aacgtgctga ctgtttatca aagcatattt gataaggatg cagacattgc
    39961 atttattttt tatcacaaaa aaagaaccac ctggatgtgt ggaactagac gcaaatcgtg
    40021 ggaaggagga aacaaggaat gcctacaatt aaccaattag tacgtaaacc tcgtaaatca
    40081 aaggtggaaa aatctaattc acctgctttg aacaaaggat ataacagctt taagaaaact
    40141 caaactaacg taaactcacc tcaaaaacgt ggggtagcaa ctcgtgtggg tacaatgaca
    40201 cctaaaaaac cgaactcagc tttacgtaaa tatgcacgtg ttcgtttgtc aaacttaatt
    40261 gaagttactg cttatatccc agggattggt cataacttac aagaacacag tgttgtatta
    40321 ttacgcggtg gtcgtgtaaa agacttacca ggggtacgtt accatatcgt tcgcggtgct
    40381 ttagatactg ctggtgttaa cgaccgtaaa caaagccgtt ctaaatacgg aactaaaaaa
    40441 cctaaagctt aattttaatt attaaacaaa tgaaacttgc ttctactata tagaagtaat
    40501 tactatgaac aatatttcgg aaggaggagt tacggatgcc acgtaaaggt cctgttgcaa
    40561 aacgtgatgt tttaccagat cctatttata actcaaaatt agtaactcgc ttgatcaacc
    40621 gtgtaatggt tgacggaaaa cgcgggattg cagctaacat tatctataat gcctttgact
    40681 tgatcaaaga atctacaggt aacgatccat tagaagtgtt tgaacaagca atgaaaaacg
    40741 ttcaacctgt tcttgaagtt aaagcacgtc gtgttggggg ttcaaactac caagtaccag
    40801 ttgaagttcg ccctgaacgc cgcgttactc taggtcttcg ttgggtagta aactacgctc
    40861 gcttgcgcgg tgaacacaca atggaagaac gcttagcaaa agaaatcatg gatgctgcaa
    40921 acaacacagg tgcttctgtt aaaaaacgtg aagacactca taaaatggct gatgccaacc
    40981 gtgcgtttgc tcactatcgc tggtaagatt ctctctgttt ggtcggcttt tataagtcga
    41041 cctcagatag tgtccttatt tttagcaact agatagtaaa gagaggagaa acaaggagaa
    41101 tgaccagaga attttcttta caaaacactc gtaatatcgg tatcatggcc cacatcgatg
    41161 ctggtaaaac gactgctact gaacgtatct tgtactatac tggtaaaatc cataaaattg
    41221 gtgaaactca tgaaggagct tcacaaatgg actggatgga acaagaacaa gaacgtggta
    41281 ttacgatcac ttctgctgct actacagcac aatggaaggg tcaccgaatc aacatcatcg
    41341 acacaccagg acacgtagac ttcacagttg aagttgaacg ttcattgcgt gtacttgatg
    41401 gtgctgtaac tgttcttgat gcacaatcag gtgttgaacc tcaaactgaa acagtttggc
    41461 gtcaagcaac aacttatggt gtacctcgta tcgtatttgc aaacaaaatg gacaagatcg
    41521 gtgctgactt cttatattca gtatcaactt tacatgaccg tttgcaagca aacgctcacc
    41581 caatccaatt accaattggt gcggaagacg acttcacagg aattatcgac cttgtaacaa
    41641 tgaaggctga aatgtataca aacgatcttg gaacagagat cgaagaaaca gaaattcctg
    41701 aagaataccg cgaattagct gaagaatggc gcgaaaaatt ggttgaagct gtagctgaaa
    41761 ctgatgaaga acttactttg aaatacttag aaggtgaaga aatcactgaa gctgagttaa
    41821 aagaaggtat ccgccgcgca acagttaacg tagaattcta cccagtacta tgtggttcag
    41881 catttaagaa caaaggggtt caattactat tggacgctgt tcttgattac ttgccatcac
    41941 cgttagatat cccagctatc aaagggattg accctaaaac tgatgaagaa gtagaacgtc
    42001 ctgcagacga cagcgctcct ttctcagctt tagcatttaa agttatgact gacccattcg
    42061 taggtcgttt aactttcttc cgtgtgtact ctggtgtttt acaatcaggt tcatacgtac
    42121 aaaacgcaac aaaaggcaaa cgtgaacgtg ttggtcgtat cctacagatg catgccaact
    42181 ctcgttcaga aatctctgaa gtttacgctg gagatatcgc tgctgctgtt ggtttgaaag
    42241 acacaactac tggggacaca ctatgtgatg aaaagagtct agttattctt gaatccatgg
    42301 aattcccaga accagttatc caagtcgcta ttgaacctaa atcaaaagct gaccaagata
    42361 aaatgggtgt ggctttacaa aaactatctg aagaagatcc tactttccgt gctgaaacaa
    42421 acgtggaaac aggcgaaaca atcatcgctg gtatgggtga acttcactta gatatcatcg
    42481 ttgaccgtat gcgtcgtgaa ttcaaagttg aagctaacgt aggtgcgcct caagtatctt
    42541 accgtgaaac tttccgtgct ggcactcaag ccgaaggtaa gtttgtacgt cagtctggtg
    42601 gtaaaggtca atacggtcac gtatggattg aatttacacc aaatgaagaa ggtgctggtt
    42661 tcgaatttga aaatgcgatc gtcggtgggg ttgttcctcg tgaatacatt ccagctgttg
    42721 aaactggttt gaaagatgct atggaaaatg gtgtcttagc tggctatcca ttagtagata
    42781 tcaaagctaa actttacgat ggttcatacc atgatgtcga ttcaaatgaa actgccttcc
    42841 gtgttgctgc ttctatggca ttacgtgcag cagctaagaa agctaatcca gtaatccttg
    42901 aaccaatcat ggctgtagaa gttgttatcc ctgaagatta tttaggtgat gttatgggtc
    42961 acgtaactgc tcgtcgtgga cgtgttgaag gtatggaagc tcgcgctggc ggccaacaag
    43021 ttgttcgtgc gatggttcca cttgctgaaa tgtttggtta tgctacaact cttcgttctg
    43081 ctactcaagg tcgtggtaca tttacaatga catttgacca ctacgaagat gtaccaaaat
    43141 cagtacaaga agaaatcatt aagaaaaacg gcggaaaagc tgaataattt cttaaaaccg
    43201 gatagtcctg caaagagatt atccaaagag actatatttt taatatagtt tcgtgtaaaa
    43261 taattatcga tgatatgggc atgtggtgta ccttgtgtac atcatatacc tatcagacaa
    43321 ataaaaaata tattgtattt ttaggaggac attttaaaat ggcaaaagaa aaatttgacc
    43381 gttctaaacc acacgttaac attggtacta tcggacacgt tgaccatggt aaaactacat
    43441 taacagctgc aatcacaact gtactatcta agaaaaacgg cggccaagct atggcttacg
    43501 atcaaatcga tggtgctcca gaagaacgcg aacgcggaat cacaatctca acagctcacg
    43561 ttgagtatga aacagataca cgccactacg ctcacgttga ctgcccagga cacgcggatt
    43621 acgttaaaaa catgatcact ggtgctgctc aaatggacgg agctatcttg gtagtttctg
    43681 ctgctgacgg cccaatgcct caaactcgtg aacacatcct attgtctcgt caagttggtg
    43741 ttccttacat cgttgtattc ttgaacaaag tagacatggt tgatgacgaa gaattactag
    43801 aattagttga aatggaagtt cgtgacctat taacagaata cgaattccct ggtgacgatg
    43861 ttcctgtagt tgctggatca gctttgaaag ctctagaagg cgacgcttca tacgaagaaa
    43921 aaattcttga attaatggct gcagttgacg aatacatccc aactccagaa cgtgacaacg
    43981 acaaaccatt catgatgcca gttgaagacg tgttctcaat tactggacgt ggtactgttg
    44041 ctacaggtcg tgttgaacgt ggacaagttc gcgttggtga cgaagttgaa gttgttggta
    44101 ttgctgaaga aacttcaaaa acaacagtta ctggtgttga aatgttccgt aaattgttag
    44161 actacgctga agctggagac aacattggtg ctttactacg tggtgttgca cgtgaagaca
    44221 tccaacgtgg acaagtttta gctaaaccag gtacaatcac acctcataca aaattctctg
    44281 cagaagtata cgtgttgaca aaagaagaag gtggacgtca tactccattc ttcactaact
    44341 accgtccaca attctacttc cgtacaactg acgtaacagg tgttgttgaa ttaccagaag
    44401 gaactgaaat ggttatgcct ggcgacaacg taacaatgga agttgaatta atccacccaa
    44461 tcgctatcga aaacggtact aaattctcaa tccgtgaagg cggacgtaca gttggtgccg
    44521 gtgtcgttac agaaatcaaa gcttaattca cttaagtttt aaagtaaagc aacatttatt
    44581 cggacgtaat cgctcggaac gaaggtctta ggatctttgt tccgagcttt tttatttatt
    44641 gcaggatcag gaaaaaagac aggagtatta taaatagaag aaacacgaaa taaaagagtc
    44701 agtattttct aaatgaaact ttatgaaaac aaaatgtttt ttacaagaaa aagccttgct
    44761 ttgtgagttt atatatatta taatgacaaa ggtgcgttta ctttagtgaa ttgcacagac
    44821 gtaatcagaa tgatttcgtc ggctaagaaa cgagaggttg cgacacgccc ggacgctttg
    44881 ccacgaacga gcgtgacgga aattttcgtg gagctatgtc tacttttcaa aataggcgaa
    44941 ggagggaaca agatggcaaa acaaaaaatt cgtatccgtt taaaagcgta tgaacaccgt
    45001 attttagatc aatcagcgga taaaatcgtg gaaactgcaa aaagaactgg agctagcgtg
    45061 tctggtccaa ttccattacc aactgaacgc agtctttaca caatcatccg tgcgactcat
    45121 aaatacaaag attcacgcga acaattcgaa atgcgtacac acaaacgtct tatcgacatt
    45181 gtgaacccaa caccaaaaac tgttgatgct ttgatgaagc tagacttacc atctggtgta
    45241 aacatcgaaa tcaaattata aaactaaaac aaaaataatg gaggtgtact catgaccaaa
    45301 ggaatcttag ggaaaaaagt gggaatgaca caaatcttca ctgaatctgg tgaattaatt
    45361 ccagtaactg ttgtagaagc tactccaaac gtagtactac aagtgaaaac aatggaaacg
    45421 gatggctacg aagctattca agttggttac caagatatgc gtgaagtttt atcaaacaaa
    45481 cctgcgaaag gtcatgttgc aaaagcaaac acggctccta agcgcttcat tcgtgaattc
    45541 aaaaatgttg agctaggaga atatgaagta ggaaaagaaa ttacagtaga tgttttccaa
    45601 gcaggagaca ttattgatgt aacaggtact acgaaaggta aaggattcca aggggttatc
    45661 aaacgccacg gacaaagccg cggaccaatg gctcacggtt ctcgttacca tcgtcgtcct
    45721 gggtcaatgg gtccagttgc acctaaccgt gtatttaaaa acaaacgtct tgctggccgt
    45781 atgggtggca accgcgtaac tatccaaaac ttggaagttg tacgtgtcga tgcagaaaga
    45841 aatgttatct tgatcaaagg taatgttcct ggagctaaaa aatctttaat tactatcaaa
    45901 tcagctgtga aagctaaata attgtaggaa gaaaggagga actaaggaat gccgaatgta
    45961 gcattattca aacaagatgg aagccaaaac ggtgaaatca cacttaacga agaaatcttc
    46021 ggaattgaac ctaatgaaag tgttgtttac gatgcaatcg taatgcaacg tgcgtcatta
    46081 agacaaggaa cacacgcggt taaaaaccgt agcgctgttc gcggcggcgg acgtaaacca
    46141 tggcgtcaaa aaggaacagg tcgtgcacgt caaggatcta tccgctcacc acaatggcgt
    46201 ggaggtggcg tagtctttgg accaacacca cgttcttaca gctacaaact tcctaaaaaa
    46261 gttcgtcgtt tagcaatgaa atctgtattg tcagaaaaag ttgctgaaaa caacttggta
    46321 gcaatcgaag gcttgaactt cgacgcacca aaaacaaaag aattcaaaca agttcttgct
    46381 aacttatcta tcgactctaa agtattggtt gttctagaaa aaggtaacga ctttgctgct
    46441 ttatcagcac gtaacttacc aaacgtttct gtagtaactt ctgataatgt gagtgtgcta
    46501 gacgttgtat caaatacaaa agtgttagca acacaaactg ctcttactca aattgaggag
    46561 gtgcttgcat aatgaactta ctagacgtaa tcaaacgccc agtgatcact gaaaaatcaa
    46621 tgcttgccat ggatgacaag aaatacactt ttgaagtgga cactcgcgca aacaaaactt
    46681 tagtaaaaca agcggtagaa gctgcttttg acgttaaagt caaaaacgtg aacatcgtga
    46741 acgtacgtgc gaaattcaaa cgtatgggca aatatgcagg atatacaaaa aaacgtcgca
    46801 aagcgatcgt tacattgact gaagattcaa aagaaatcca attattcgaa gctgctgaat
    46861 aagcactaat agataactca ttaaattagg agggaaaaac gtggcgatta aaaagtacaa
    46921 acctacctca aatggtcgtc gtaacatgac aggttctgat tttgctgaaa tcacaacatc
    46981 aacacctgaa aaatcattgt tacaaccatt gaaaaaccat gctggacgta acaacaacgg
    47041 ccgcatcact gtacgtcatc aaggtggcgg acacaaacgc caatatcgcg tgatcgactt
    47101 caaacgtaac aaagataacg ttgttgcaac tgttcaaacg atcgaatatg atccaaaccg
    47161 ttcagctaac atcgcgttag tacactatga agatggagtg aaagcttaca tcttagcacc
    47221 taaaggacta gaagtgggta tgaaacttgt ttcaggtcca gaagcagata tcaaagtcgg
    47281 aaatgctctt ccattagaaa acattccagt tggtactgtt atccacaaca ttgaaatgaa
    47341 acctggtaaa ggtggacaat tgatccgttc tgctggtaca agtgctcaag tacttggtaa
    47401 agaaggcaaa tacgtattga tccgcttgaa ctctggtgaa gttcgtatga tcttagctgc
    47461 atgccgtgct acaatcggtt ctgtaggtaa cgaacaacac gaattaatca acatcggtaa
    47521 agctggacgc tctcgttgga tgcgtaaacg cccaactgta cgtggtagcg taatgaaccc
    47581 taatgatcac ccacacggtg gtggtgaagg taaagctcct atcggacgta aagcaccagt
    47641 ttctccttgg ggtcaaccag ctcttggtta caaaactcgt aacaaaaaag ctaaatcaga
    47701 caaacttatt gttcgtcgtc gcaaaaataa ataattactt tgaccatgtg tcgcacattt
    47761 tgagaggagg ttcaccatgg gtcgtagttt aaagaaagga ccttttgtcg atgagcattt
    47821 aatgaaaaaa gttgaagctc aagcaggggt tgaaaagaaa aaagttatca aaacttggtc
    47881 acgccgttca acagttttcc caagttttgt tggatatacc atcgcagttt atgatggacg
    47941 taaacatgta ccagtttaca tccaagaaga catggtagga cacaaattag gtgaatttgc
    48001 acctacaaga acttatcgtg gacatgctgc tgacgataag aaaacgaaac gctaatttga
    48061 ggggaggata aatccaaatg gcagaacaaa tcacatcagc taaagcaact gcaaaaacag
    48121 ttcgcacttc acctcgaaaa gcccgtttag taattgacct tatcagaggt aaaagtgtcg
    48181 cagatgcaat ttcaatcttg aaattcacac caaacaaatc tgctggaatc attgaaaaag
    48241 tattgatgtc agcagttgcg aatgcagaaa ataactttga cttagatgtt gaaaacttgg
    48301 tagtaagcga agcttttgtt aacgaaggac ctacaatgaa acgtttccgt cctcgtgcaa
    48361 aaggatcagc ttctccaatc aacaaacgta caagtcatat cacggtagtc gtatcagaaa
    48421 aataaggagg gataatcagt gggtcaaaaa gtacatccaa ttggaatgcg tgtaggcatc
    48481 atccgcgact gggatgctaa atggtatgct gaaaaagagt atgctgagtt cttacacgaa
    48541 gatttaagaa tccgtaaatt tatttcaact aaacttgctg atgctgctgt atcaacagtt
    48601 gaaatcgaac gcgcaacagg tcgcgtaaac atttcaattc acacagctaa accaggtatg
    48661 gttattggta aaggcggttc tgaagtcgaa agcctaagaa aagaattaaa caaattaact
    48721 ggtaaaagaa tccatatcaa tatcgtggaa atcaaaaaac cagatctaga tgcaaaatta
    48781 gtaggtgaag gaatcgcacg tcaattagaa aaccgtgttg ctttccgtcg cgctcaaaaa
    48841 caagccatcc aacgctcaat gcgtgctggc gctcaaggga tcaaaactca agtatctggt
    48901 cgtctaaacg gagcagatat cgctcgttca gaaggatact cagaaggaac tgttcctctt
    48961 catactttac gtgcggacat tgattacgca tgggaagaag cagatacaac atacggaaaa
    49021 ctaggagtta aagtgtggat ctatcgtgga gaaattcttc caactaaaaa aaacactgag
    49081 aaaggaggga aataatcatg ttagtaccta aacgtgtaaa acaccgtcgt gaattccgcg
    49141 gaaaaatgcg cggggaagct aaaggcggaa aagaagtagc attcggtgaa tacggtttgc
    49201 aagctgttga ttcacattgg atcacaaacc gccaaatcga agctgctcgt atcgcaatga
    49261 ctcgttacat gaaacgtggt gggaaagtat ggattaaaat tttccctcac aaatcttata
    49321 ctgccaaagc aattggggta cgtatgggtt ctggtaaagg ggcacctgaa ggatgggttg
    49381 caccagtaaa acgtggtaaa atcatgtttg aaatcgcagg cgttcctgaa gaagtagctc
    49441 gtgaagcgtt acgtctagct tctcacaaat taccaatgaa aactaagatc gtaaaacgtg
    49501 aggaaatggg tggtgaatcg aatgaaggtt aaagaaatca gagaattaac cactgccgaa
    49561 atgcttgatc aagaaaaaca attaaaagaa gaattgttta atcttagatt ccaattagca
    49621 acaggtcaat tagaaaacac tgcacgtatt aaagaagtac gtaaatcgat tgcacgcatc
    49681 aaaactgtgt tgcgtgaaca agctaagtaa ttatggaagg aggccattaa gcgtatgact
    49741 gaaggaagaa atcaacgcaa agtttatcaa ggtcgcgtgg tttcagacaa aatggataaa
    49801 acaattaccg ttgctgtcga aacaaagaaa aaccacccta tctatggcaa acgcatgaac
    49861 tattctaaga aatacaaagt gcatgatgaa aacaacacag caaaagttgg cgacatcgtg
    49921 agaattatgg aaactcgtcc attatcagct acaaaacgtt tccgtttgtt ggaagtagtc
    49981 gaagaagcag ttattatcta ataacacgag attttcctat atagattgaa tgcaaaatct
    50041 gaaaggagga tacacaacgt gatccaagca gaaagtcgtt taaaagtcgc tgataactca
    50101 ggcgcacgtg aaattttgac gatcaaagtc cttggtggat ctggtcgtaa aactgctaac
    50161 atcggtgatg tgatcgttgc tacggttaaa caagcaacgc caggtggggt tgtcaaaaaa
    50221 ggtgacgtcg ttaaagccgt tatcgttcga acaaaatcag gagctcgtcg tacagacggt
    50281 tcatatatta aatttgatga aaatgctgca gtgattattc gtgatgataa aagccctcgt
    50341 ggaacacgta tctttgggcc agttgcacgt gaactacgtg aaaacaactt catgaagatc
    50401 gtttcactag caccagaagt tttataatct tgagacactt atcaaaggag gtgcgaaaca
    50461 gttatgtttg ttaaaaaagg cgataaagtt aaagttatca ctggaaaaga caaaaacaaa
    50521 gaaggcgttg tactagcagc gttcccgaaa aaagataaag ttattgtcga aggtgtaaat
    50581 gtcgttaaaa aacaccaaaa accttctcaa gctgctcctc aaggtggaat tgttgagatg
    50641 gaagcgccaa ttcatgtttc taacgtgatg gtggttgact ctactggtgt agctggccga
    50701 gttggctaca aagaagtaga cggcaaaaaa gtccgtgttt ctaaaaaaac cggtgaagtt
    50761 ttagataaat aaaagtttaa ggaaggaggg tcttactgaa tgaaccgcct aaaagaaaaa
    50821 tatcttaaag aagtaactcc atcattgatg gaaaaattta actatagctc tgttatgcaa
    50881 acacctaaag ttgaaaaaat cgtcatcaac atgggtgttg gtgatgcggt atcaaacgct
    50941 aaaaacttag ataaagcagt tgaagaatta actttgatca ctggtcaaaa accaatgatt
    51001 acaaaagcta aaaaatcaat cgctggattc cgtttacgtg aaggaatgcc aattggtgca
    51061 aaagttacat tgcgcggaga aagaatgtac gaattcttag acaaattggt ttctgtttca
    51121 ctaccacgtg tacgtgactt ccatggtgta agtaaaaaag cctttgacgg tcgtggaaac
    51181 tatactttag gtattaaaga acaattgatc ttcccagaag tagattatga tttagtagat
    51241 aaagtacgtg gtatggacat cgttattgta acaacagcaa actctgatga agaatctcgt
    51301 gagttgttga cacaattagg catgccattc caaaaataat taaaggaggc gaactacgtg
    51361 gctaaaaaat caatgattgc taaaaacaaa cgtcctgcaa aattttcaac acaagcttat
    51421 actcgttgcg aacgttgcgg acgtccacat tcagtttatc gtaagttcca tctttgccgt
    51481 atttgcttcc gcgaacttgc ctataaaggt caaattcccg gcgtgaagaa agctagctgg
    51541 taaaaagtaa attcgcataa gaaggaggta aaagatcaat ggtcatgaca gatccaattg
    51601 cagattttct aactcgtatc cgtaacgcaa acatggttaa acatgagtct ttagaagtac
    51661 ctgcatcaaa aattaaacgt gacatcgctg aaatcttgaa acgtgaaggt ttcgttcgtg
    51721 atgttgaata catcgaagat gacaaacaag gcgtgatccg tgttttccta aaatttggta
    51781 aaaacggcga acgtgttatc acaaacttaa aacgtatctc taaaccaggt ttgcgtgctt
    51841 atgtcaaagc tgacgaagta ccaaaagtat tgaacggttt aggtatcgca atcatttcga
    51901 cttctgaagg tgttgtcact gataaagaag ctcgtgaaaa aaatatcggc ggcgaagtta
    51961 tcgcttacgt atggtaatca agtagaatct acaaggaggt gtctttaagt gagccgtatc
    52021 ggaaataaag tcgttgttat ccctgaagga gtaacagtta ctcaagacgg aaacaacgtt
    52081 acagttaaag gaccaaaagg tgaattgact cgtacttttt cagcagatat caaaatgaat
    52141 atcgaaggaa acgaagtaac tttcactcgt ccaaacgaca gtaaagaaat gaaaacaatt
    52201 catggaacaa ctcgtgcgaa cttcaacaac atggttgtag gtgtcagcga aggtttccaa
    52261 aaagcgttag aactaatcgg ggttgggtac cgtgcacaat tacaaggatc aaaacttgta
    52321 ttgaacgttg gttactcaca tccagtagaa atcacaccac cagctggagt aactgttgaa
    52381 gtaccagcta acacacaagt aattgttaaa ggtgcgaaca aagaagaagt tggcgaatta
    52441 gctgcaaaca ttcgtggaac tcgtcctcca gaaccttata aaggtaaagg tattcgttat
    52501 gttggcgaat atgtacgccg taaagaaggt aaaactggta aataatcgtt tagaaaaatt
    52561 gcatagaagc cagcagcagg aaaaaggcat ttggctgaag tttaccttca aaaaaataca
    52621 tttttacttg tgccgcagct tctatttttc gaaacgtatt tactgcttga aatagtagta
    52681 caataaaaat aaagaggtga caattgtgat tacaaaacca gataagaaca aaacacgtca
    52741 aaagagacat cgtcgtgtac gtaacaatat ctctggtact gctgagcgcc cacgcttgaa
    52801 cgtttttcgt tctaacaaaa acatctacgc tcaaattatt gatgacgtag cgggtgtaac
    52861 gctagcaagt gcctctacct tggataaaga aatttcaggt ggaacaaaaa cagaagcagc
    52921 acaagctgtc ggtaaactag ttgctgaacg tgcagctgaa aaaggcatta aagtagtagt
    52981 ctttgaccgt ggtggatacc tttaccatgg ccgtgtgcaa gctttagctg aagctgctcg
    53041 cgaaaatgga ctagaatttt agaagaagga ggaataccat tcatggttta tatcgatcca
    53101 aaacatttgg aattagaaga ccgcgttgtt gcaattaacc gtgtaacaaa agttgttaaa
    53161 ggtggacgtc gtctacgttt tgctgcttta gttgttgtcg gtgacaaaaa cggacacgta
    53221 gggtttggta caggtaaagc tcaagaagtt cctgaagcaa tccgtaaagc aattgaagat
    53281 gcgaagaaaa acttagttga agtacctatg gttggttcaa caatcccaca cgaagtaatc
    53341 ggtgctttta gtggtggtag aatcctaatg aaacctgctg tagaaggttc tggggttgct
    53401 gctggtggac cagttcgtgc cgtattagaa ttagctgggg tagcagatat cacttctaaa
    53461 tctttaggct caaatacacc aatcaacgtt gttcgcgcaa cagttgaagg tttgaaacaa
    53521 ttgaaacgtg cagaagaagt tgcagaactt cgcggcaaat cagttgaaga attaatcgga
    53581 taaggaggac aacaataatg gctgaattaa aagttacttt aaaacgcagt attatcggac
    53641 gtcctcaaaa ccaacgcgat acagttaaag cgttaggtct tacaaagatt aacagttcag
    53701 tggttaagcc ttctaatgaa gcaattaaag gcatgatcaa tactgtttct cacctagtgg
    53761 acgttgaaga aatttaattt agaaaaatca taactagaac attaaggagg tgccaaacct
    53821 atgaaacttc atgaattaaa acctgctgaa ggttcacgcc aagtacgtaa ccgtgttggt
    53881 cgtggtactt catctggtaa cggaaaaact gccggacgcg gacaaaaagg acaaaaagct
    53941 cgttcaggcg gcggtgtgcg tttaggattt gaagggggac aaacaccatt gttccgtcgt
    54001 ttaccaaaac gaggattcac aaacgtgaac cgtaaagact atgcagtcat taatcttgat
    54061 gtattgaacc gctttgaaga cggagctgaa gtaactcctg ttgctttagt tgaagctgga
    54121 atcgtgaaaa acgaaaaagc tggaatcaaa gtattagcta atggggaact aaacaagaaa
    54181 ttaactgtga aagcagcaaa attctctaaa gcagctcaag aagcaattga agcggctggt
    54241 ggatcaatcg aggtgatcta atgtttagac tattaaaaga tgcttttaaa gtaaaagaca
    54301 ttagatcgaa aatcctattt actgtatttg tgttgtttgt atttcgccta ggtgcacata
    54361 ttactgtacc aggcgtaaat gctgcttcat tgcaaaacct ttcttctttg ccatttttca
    54421 acatgttgaa cttggtaagt ggtagtgcga tgcaaaactt ttccattttc tcaatggggg
    54481 tttcaccata cattactgct tctatcgtca ttcagcttct tcaaatggac atcgtgcctc
    54541 gatttgttga atggtcgaaa caaggggaag ttggtcgtaa aaaactgaat caagcgactc
    54601 gatatctgac attagtattg gcatttatcc aatctgtcgg tatcactgct ggattcaatc
    54661 aatacgctca attaggtttc gtgaataatc ctaacgtagc aacttacgta acgatcggtt
    54721 tgattctaac agccggtacg atgtttgtta cttggctagg agaacagatc acagaaaaag
    54781 gtatcggaaa tggcgtatca atgatcattt ttgcaggtat catttctaga ttgcctgaaa
    54841 gtatcagaga aatttatgaa gattacttta taaatattga tccttcagac atatggaaat
    54901 ctgctatttt tgtggccatt ttactagtag cggtcttgtt gatcgtgaca ttcgtaacat
    54961 ttttccaaca agctgaacgc aagatcccga tccaatatac gaaacgcgta gctggcgcgc
    55021 ctacaagcag ctatctgccg ttgaaagtaa atgcagctgg agttattcca gttatctttg
    55081 ctagttcgtt tattgctacg ccaaatgctg ttttacaagc attgagcggt agattcggtt
    55141 ctgaaaactg gtacaaagtg atgatggaga tctttaacta taatacagca cctggtgcta
    55201 cgatttatac gattctgatc gttgccttca ctttcttcta cgcctttgtt caagtaaacc
    55261 ctgagaaatt agcggaaaac ttgcaaaagc aaggaagtta tataccaagc gtacgccctg
    55321 gtaaggggac tgaagagtat gtctcaagat tactgatgcg tttaagtaca gttggtgcag
    55381 tcttccttgg tttagtggca ttgcttccaa tcattgctca aatggtttgg gatcttccac
    55441 aatcaatcgg attaggcggt acaagcttac tgatcgttat cggtgtagct ttagaaactg
    55501 cgaaacagtt agaaggctta atgctgaaac gtaaatatgt tggattcatt gagtaagaaa
    55561 gcgtgttgag gtttcccctc aacacacgtt ctcacacatt tattaggagg gaaaacaatg
    55621 aacctcattt taatggggct gccaggcgca ggtaaaggaa cacaagctga aaaaatcatt
    55681 gatgcttatg gtattccgca catctcaaca ggagatatgt tccgcgcagc gatgcaaaac
    55741 gaaacagctc ttggcctgga agcaaagtct tatatggata aaggtgcatt agttcctgat
    55801 gaagtaacaa atggaatcgt gaaagagcga ctagctgaac cagatacaga gaaaggcttc
    55861 ttattagatg gctttccacg tacgctagat caagcaaaag ctctggacgc aatgctaaaa
    55921 gatttgaata aaaaaattga tgctgtcatc gatatccatg ttggtgaaga aatcttggtc
    55981 gaacgtctag ccggccgatt tatctgcagc aattgtggag caacttacca taaaattttc
    56041 aaccctacaa aagttgaaga cacatgtgat cgttgcggtg ggcatgaatt ctatcaaaga
    56101 gaagatgata aacctgagac ggttaaaaat cgtctggcta tcaacatcaa aaatagtgaa
    56161 ccaatcttgg cttattataa agaacaaggt ttgctaaaca ctattgatgg tgctcgtgaa
    56221 atcgatgctg tatttgcaga tgtacaaaaa atcattgaaa aatagcacga ttgcccgtag
    56281 ccgacttgca ttatgcagtt actcttgtca agttcaataa attatgatag aatatgtcag
    56341 tccgacttct agagaatcct ctagatgatc cattttgaga attttgaggt gggaactgca
    56401 gtttccgcca ttttatcgtg tgaaaatgcg aaacaagtac aaggaggtac tgtgcgtggc
    56461 aaaagaagat atgattgaag tcgaaggtac agtcgtcgaa actttgccga atgcaatgtt
    56521 taaagttgaa ctagaaaacg gacaccaagt tcttgctact gtttcaggta aaattcgtat
    56581 gcattacatc cgtatcttac ctggtgacaa agtaacggtt gaattgtctc catacgatct
    56641 taaccgtggc cgtattactt atcgctttaa ataattgtac tccgtaaaac tacaggaggt
    56701 attatcatga aagtaagacc atcagtaaaa ccaatgtgcg aacattgtaa agtaattcgt
    56761 cgtaaaggac gcgttatggt gatttgccca gcaaatccaa aacataaaca acgtcaagga
    56821 taattggagg tgctataaat atggctcgta ttgcaggagt agatattcct cgtgataaac
    56881 gtgtagtagt ttcacttact tatatctatg gtattggtaa cactactgct aaaaagatct
    56941 tagctgatgc tggtgtatct gaagatgttc gcgttcgtga attaacgaat gaacaaacag
    57001 atgctatccg tgcagaagta gataaattaa aagttgaagg ggatcttcgt cgtgaagtga
    57061 acttgaacat caaacgtttg atggaaatcg gttcttaccg tggaatccgt catcgccgtg
    57121 gattgcctgt tcgtggacaa aacacgaaaa acaatgcacg tactcgtaaa ggcccagctc
    57181 gcgctatcgc tggcaagaaa aaataattct taagtgaagg aggttaaaac ttcatggcag
    57241 caaaaaaagt gaatcgtaaa cgccgtgtga agaagaatat agaagcaggt attgcacata
    57301 tccattctac attcaataac actatcatca tgatcactga tactcacgga aatgcattag
    57361 cgtggtcatc agctggttca ttaggtttca aaggaagcaa aaaatcaact ccttttgccg
    57421 ctcaaatggc agcagaaact gctacaaaag cagcaatgga acacggatta aagactgttg
    57481 aagtaacagt taaaggacct ggttctggac gtgaagcagc aattcgttca ttacaagcta
    57541 caggtttaga agtgactgca attcgtgacg tgactccagt tcctcataat ggtgcccgcc
    57601 ctccaaaacg ccgtcgtgtt taatgagtgc cgctcatttt gagttttatc agtaacgtca
    57661 ttgaacaaga cacttttcgt tttgaaaggg gtaaagataa gaatgattga gttcgaaaaa
    57721 ccaagaatcg caaaaattga tgaagaaaaa gattatggca agttcatcgt tgaacctctt
    57781 gaaagagggt atgggactac tttagggaat tccctacgtc gtattttatt atcttctttg
    57841 ccaggtgctg ccatcactaa tattcaaatc gatggtgtac tgcatgaatt ctctaccatc
    57901 aaaggtgtta gagaagacgt cactcaaatc atcttgaaca ttaaaggctt agcgctgaaa
    57961 atgtatggcc aagaagaaaa aacccttgaa atcgatatta ccggacctgc tacagtaact
    58021 gctggcgata ttatcgttga tagtgatgta gaaattctta acaaagatat gtacatctgt
    58081 agtgtctctg aaggagctac gttccatgct cgcttaacag tgaaacctgg acgtggttat
    58141 gttcaagcag atgaaaataa aaaagaagat atgccaattg gcgttcttcc agtcgattca
    58201 atctacacac ctgttcgtcg tgtgaactat caagttgaga acacacgtgt tggacaccgt
    58261 gaagattttg ataaattgac gatggagatt tggacagatg gttcaattga accgttggaa
    58321 gcaatgagtt tgtctgcgaa aatcatgact gaacatttgg atatttttgt gaacttaact
    58381 gatgaagcaa aaaatgctga aatcatggtc gaaaaagaag aaacacaaaa agaaaaaatg
    58441 ttggaaatga cgatcgaaga gttagacttg tctgttcgct cttacaattg tttgaaacgt
    58501 gcaggaatca atactgtaca agaacttaca aataaatctg agcctgaaat gatcaaagtc
    58561 cgtaacttag gacgtaaatc attagaagaa gttaaagcaa aattgcacga cttaggttta
    58621 ggtttacgta aagaagatta aaacctttta cgaaggaggg aaaccaacgt gagttatcgt
    58681 aaactaggac gcacatctag ccaacgtaaa gcgatgttgc gtgatttaac aactgattta
    58741 ttaatcaacg aacgtattgt gactactgaa gctcgtgcga aagaagttcg ctcaactgca
    58801 gaaaaaatga tcactttagg taaacgcgga gatttgcatg cacgtcgtca agctgcggct
    58861 ttcgtacgta atgaagttgc cagcgtgcgt gaagaaaatg aggacatcgt cattgaatca
    58921 gctttacaaa aactgttcaa cgatattgca cctcgttacg cagaacgtca aggcggatac
    58981 acacgcattt tgaagacaga accaagacgc ggtgatggtg caccaatggt tgtcttagaa
    59041 cttgtctaaa atcacactaa taacatcact ttatagatga ttcttttaga gtgttatgat
    59101 gttggaagat atttttccga gtctagctca gcgctgctcc tcaagcgaat atcgcttggg
    59161 gagtaagcac tcatcataaa gtgttttttt ataccttcaa ttacctgtca tgacagatat
    59221 gtttgaaggc ataaaaaaac attttaagcg agaaataatt gaaaattttg tctttttttg
    59281 atagtgttag taggaacttt aggatgaaga aggagtaatc atggagccaa ttattgaatt
    59341 agaaaagata aattataaat accagccaga tgatcttcgc cccgcattga aagatgtctc
    59401 ctttacaatt gataaaggtg agtggatcgc tattattgga cacaatggtt caggaaaatc
    59461 gacacttgcc aaaacgatca atggattgct tctgccagaa tcagggatcg tgaaagtggg
    59521 aaatcaaata ttggatgaag aaaatatttg gacgatccga cagatggttg gaatggtttt
    59581 tcaaaatcca gacaatcagt ttgttggttc gacagtagaa gatgacgttg cttttggtct
    59641 tgaaaatcaa gggattccca gagaagaaat gctagtgcgt gtgaaagatg cacttgagaa
    59701 agttagaatg gctgagtttg cttcacgcga gcctgctcgt ttatcaggag ggcaaaaaca
    59761 acgtgttgcc attgccggcg tggttgcctt acgacctgat atcattattt tagatgaagc
    59821 aaccagtatg ctagatccag aaggtcgtga agaagtgatc tctacgatca agaagatcaa
    59881 agaagaaagt caattgaccg ttatatctat cactcatgat attgatgaag cagctaatgc
    59941 caatcggata ctagtgatga gacaaggcga acttgtccgt gaaggtacac caaaagaaat
    60001 tttttctgct ggtcctgaac tgatcgactt aggtctagat ctaccatttc ctgaaaaact
    60061 gaagagcgca ctgaaagaac gtggggttga tgtgcccagt gaatatatga cagaagaaag
    60121 gatggtggat tggttatgga catccgtttt gaacaagtag acttcactta tcagccgaat
    60181 acaccatttg aacaaagagc cttatttgac atcaatatga cgatcaaaga aaacagttat
    60241 actgcattag tagggcatac cggaagcggg aaatcaacct tacttcagca tttgaatgcg
    60301 cttgttaagc caacaagcgg tacagtccat attggagaac gagatattca gccggatacg
    60361 gataataaga atttaaagcc catccgaaaa aaagttggca tcgtctttca gtttccagaa
    60421 gcacagctat tcgaagaaac ggtagcaaaa gacattgctt tcggtcctaa aaactttggt
    60481 gtcagcgaag aagaagcact agtcctagca aaagaaacat tagaacaagt tgggctggat
    60541 gaaagctatt tggaacgttc gccatttgaa ctttcaggag ggcaaatgcg tagagtagca
    60601 atcgctggtg tgcttgccat gaggccggaa gtacttgtat tggatgaacc aacggcagga
    60661 cttgatcccc agggtagaaa agaaatgatg gagatgtttt ggcgtcttca taaggaacag
    60721 cagatcacta ttgtattagt tacgcattta atggatgatg tagccaattt tgcagattat
    60781 gtctatgtgt tggaaaaggg acgaatcgtt aatagcgggg agccgcaaga ggtctttcaa
    60841 aacattgaat ggctaaaaga aaaacaacta ggcgtaccta ctgccaccga gtttgcagaa
    60901 gaattaatgg ctaaagggat gaactttgct accttgcctt tgacagctga agaattagca
    60961 gacgctatcg tccattatgc tggaggaaat gcccatgatg aataaattga tatttggtcg
    61021 ttatatgcct ggagattcct tgatccatcg attggatccc cgagctaaat tattagccag
    61081 tttttatttt atcggaatca tttttttagc aaataattgg gaaacgtacc ttttgttggc
    61141 atttttcaca ttactttctg tcttcttgtc aaaagtagat ttgaaatttt ttatccgagg
    61201 cattcgccca ttgatctggc taatcctctt cactgtagct ttgcaggtct tgtttacaag
    61261 tggcggagaa acttattgga gttggggtgt tttccatatc actgaatttg gtgtgatgaa
    61321 cggcctcttt attttctgtc gattcgtgtt gatcattttt atgtcgactc tactcacgct
    61381 tactacaccg cctttggaat tatcggatgc gattgagtac ttgcttcgtc cattgaaagc
    61441 cgtacggttc ccagtccacg aaatttcatt gatgctgtcg attgctcttc gttttgtgcc
    61501 tacattgatg gacgaaacag aaaagatcat gaacgctcag cgcgctcgcg gtgttgattt
    61561 tggtgaagga aacctagttc agaaaatgaa agcagtcatt cctttgctga ttcctttatt
    61621 tgtcagcagc ttcaaccgtg cggaagatct tgcgactgct atggaagcaa gaggctacaa
    61681 aggtggggaa ggaagaacga aatatcgtgt attgcattgg cataatagag acacgatggt
    61741 tatcattgct tttgtattat taactgtgct actgatcttt ttgagaggat aaacgttcag
    61801 tgggccgcta tctattcttt tcttgctgat ctgtttgcaa gaaaggatgc gagcagtgta
    61861 tgacaagaaa tattagggtc tgtggcagac taaggtcaca ggcctctttc ttttatctga
    61921 aaagaaagaa cgaaaaaatt ttataaaaaa atcattattc cagataacct tttaggaaca
    61981 aaggagagaa aaacatggta cgttacaaag caatcattgc atacgacgga acaaatttta
    62041 atggattcca aaaacagcca aatgggcgta ctgttcaaga agaagtagaa aaaacattac
    62101 aaaaaatggc aaatggaaaa gaaatcacgg tctttggttc gggaaggaca gatgccggag
    62161 tacatgcgat tgggcaagta atccattttg attatccaga ggagcgtccg ttagaacgaa
    62221 tgcgtttcgc gctagatacc cagtcaccag aggatattgc tgtaagacaa gtagaaattg
    62281 tttcagaaga cttccacgca agatatttgg tcaaagaaaa gacgtatcaa tttcgagtgg
    62341 atatagggaa accacgcagt ccttttcgac gccattatgc aagttatttt ccttaccctc
    62401 ttgatttgga aaaaattcag cgggcgcttc ctgatttatt agggactcac gatttcacct
    62461 ctttttgtgc atcaggaagc tcgatagagg ataaagtacg tacgatctac gaggctaaga
    62521 tggaggtaaa tgaaacaaaa gacgaattgt tgtttacatt taggggaaat ggcttcttgt
    62581 ataagatgat acggatctta gtaggaacgc ttttgaaaat tggcaatggc cgattgcctg
    62641 aagatagtat cccagatatt attgcgaaaa aagatcgtaa tgcagcagga cctacagcac
    62701 atcctgaagg actttatttg tatgaagtga aatatgaata agaggttgtg acagaagtgg
    62761 tcagcttcaa gaaataagaa ggaatttacg aaaattactt ctcaaaattt tgcaaaattt
    62821 cggcttattt tcgaaggagc tacttctgga acaccgtgaa tcagaggttg tgaaaaagct
    62881 gccctgcatc aagtaataag aacaaccaaa caaaaatagc tcgtcatatt tttcgtttgg
    62941 ttgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat
    63001 cagcgttttg acctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg
    63061 tttgttgagc taataccgaa ggtcagctga ttgaacaccg tttaagaaag aggtcgtgag
    63121 cctgtcattt aactctgagt cacaagtaac aatttcaaaa aacagcttct catctttgag
    63181 gaatatcgtg acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt
    63241 ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc cttaatgagg acagtcggaa
    63301 caggtttgtc atttttat
//
LOCUS       DACCDF010000007        56296 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCDF010000007 DACCDF010000000
VERSION     DACCDF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.007"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..189
                     /locus_tag="IVK51_000410"
     CDS             <1..189
                     /locus_tag="IVK51_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6430032.1"
                     /translation="NSNGILRRNGLPKSIDFREVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
     gene            complement(1055..1585)
                     /locus_tag="IVK51_000411"
     CDS             complement(1055..1585)
                     /locus_tag="IVK51_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6430033.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1582..2199)
                     /locus_tag="IVK51_000412"
     CDS             complement(1582..2199)
                     /locus_tag="IVK51_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6430034.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
     gene            complement(2781..3002)
                     /locus_tag="IVK51_000413"
     CDS             complement(2781..3002)
                     /locus_tag="IVK51_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HAP6430035.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
     gene            complement(3180..4712)
                     /locus_tag="IVK51_000414"
     CDS             complement(3180..4712)
                     /locus_tag="IVK51_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6430036.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            complement(4848..5933)
                     /locus_tag="IVK51_000415"
     CDS             complement(4848..5933)
                     /locus_tag="IVK51_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6430037.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            complement(5948..6667)
                     /locus_tag="IVK51_000416"
     CDS             complement(5948..6667)
                     /locus_tag="IVK51_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6430038.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            complement(6664..7974)
                     /locus_tag="IVK51_000417"
     CDS             complement(6664..7974)
                     /locus_tag="IVK51_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP6430039.1"
                     /translation="MRISIKRWSGADWTLFTLAFFAISIVLVYTFNAPRAILYLGDII
                     NIFVLLNALSFRRRKGIYASKNEMLIVLFLLVSICSSVINLENIGLIIWGIRNNTRMF
                     IVYINCATYLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFWN
                     YSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELFI
                     IFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLLE
                     YASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKSF
                     GNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSVF
                     FFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            complement(8001..8591)
                     /locus_tag="IVK51_000418"
     CDS             complement(8001..8591)
                     /locus_tag="IVK51_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HAP6430040.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(8909..9970)
                     /locus_tag="IVK51_000419"
     CDS             complement(8909..9970)
                     /locus_tag="IVK51_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6430041.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(10538..11605)
                     /locus_tag="IVK51_000420"
     CDS             complement(10538..11605)
                     /locus_tag="IVK51_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6430042.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(11625..12104)
                     /locus_tag="IVK51_000421"
     CDS             complement(11625..12104)
                     /locus_tag="IVK51_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HAP6430043.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(12121..12582)
                     /locus_tag="IVK51_000422"
     CDS             complement(12121..12582)
                     /locus_tag="IVK51_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6430044.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(12596..13846)
                     /locus_tag="IVK51_000423"
     CDS             complement(12596..13846)
                     /locus_tag="IVK51_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAP6430045.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(14224..15675)
                     /locus_tag="IVK51_000424"
     CDS             complement(14224..15675)
                     /locus_tag="IVK51_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6430046.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(15996..16760)
                     /locus_tag="IVK51_000425"
     CDS             complement(15996..16760)
                     /locus_tag="IVK51_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP6430047.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(16788..17486)
                     /locus_tag="IVK51_000426"
     CDS             complement(16788..17486)
                     /locus_tag="IVK51_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP6430048.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(17498..18277)
                     /locus_tag="IVK51_000427"
     CDS             complement(17498..18277)
                     /locus_tag="IVK51_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP6430049.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(18293..19237)
                     /locus_tag="IVK51_000428"
     CDS             complement(18293..19237)
                     /locus_tag="IVK51_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6430050.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(19283..20062)
                     /locus_tag="IVK51_000429"
     CDS             complement(19283..20062)
                     /locus_tag="IVK51_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430051.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(20442..22517)
                     /locus_tag="IVK51_000430"
     CDS             complement(20442..22517)
                     /locus_tag="IVK51_000430"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP6430052.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IVK51_000431"
     CDS             complement(22519..23436)
                     /gene="glyQ"
                     /locus_tag="IVK51_000431"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP6430053.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IVK51_000432"
     CDS             complement(23822..24640)
                     /gene="recO"
                     /locus_tag="IVK51_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP6430054.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(24783..25682)
                     /locus_tag="IVK51_000433"
     CDS             complement(24783..25682)
                     /locus_tag="IVK51_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP6430055.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(25697..26098)
                     /locus_tag="IVK51_000434"
     CDS             complement(25697..26098)
                     /locus_tag="IVK51_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP6430056.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IVK51_000435"
     CDS             complement(26076..26552)
                     /gene="ybeY"
                     /locus_tag="IVK51_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP6430057.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(26581..28773)
                     /locus_tag="IVK51_000436"
     CDS             complement(26581..28773)
                     /locus_tag="IVK51_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP6430058.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(28797..29768)
                     /locus_tag="IVK51_000437"
     CDS             complement(28797..29768)
                     /locus_tag="IVK51_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP6430059.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            30473..31852
                     /gene="brnQ"
                     /locus_tag="IVK51_000438"
     CDS             30473..31852
                     /gene="brnQ"
                     /locus_tag="IVK51_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6430060.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(32008..32454)
                     /locus_tag="IVK51_000439"
     CDS             complement(32008..32454)
                     /locus_tag="IVK51_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP6430061.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(32481..32657)
                     /locus_tag="IVK51_000440"
     CDS             complement(32481..32657)
                     /locus_tag="IVK51_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP6430062.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(32819..33247)
                     /locus_tag="IVK51_000441"
     CDS             complement(32819..33247)
                     /locus_tag="IVK51_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6430063.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            33339..34199
                     /locus_tag="IVK51_000442"
     CDS             33339..34199
                     /locus_tag="IVK51_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP6430064.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(34293..34586)
                     /locus_tag="IVK51_000443"
     CDS             complement(34293..34586)
                     /locus_tag="IVK51_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP6430065.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(34659..36386)
                     /locus_tag="IVK51_000444"
     CDS             complement(34659..36386)
                     /locus_tag="IVK51_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP6430066.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IVK51_000445"
     CDS             complement(36599..37525)
                     /gene="trxB"
                     /locus_tag="IVK51_000445"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP6430067.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IVK51_000446"
     CDS             complement(37670..39103)
                     /gene="ascB"
                     /locus_tag="IVK51_000446"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6430068.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(39103..40506)
                     /locus_tag="IVK51_000447"
     CDS             complement(39103..40506)
                     /locus_tag="IVK51_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP6430069.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            40736..41638
                     /locus_tag="IVK51_000448"
     CDS             40736..41638
                     /locus_tag="IVK51_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6430070.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(41679..43565)
                     /locus_tag="IVK51_000449"
     CDS             complement(41679..43565)
                     /locus_tag="IVK51_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6430071.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(43662..44514)
                     /locus_tag="IVK51_000450"
                     /pseudo
     CDS             complement(43662..44514)
                     /locus_tag="IVK51_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(44849..46633)
                     /locus_tag="IVK51_000451"
     CDS             complement(44849..46633)
                     /locus_tag="IVK51_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430072.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(46633..48384)
                     /locus_tag="IVK51_000452"
     CDS             complement(46633..48384)
                     /locus_tag="IVK51_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430073.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(48526..48750)
                     /locus_tag="IVK51_000453"
     CDS             complement(48526..48750)
                     /locus_tag="IVK51_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP6430074.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            49001..50587
                     /locus_tag="IVK51_000454"
     CDS             49001..50587
                     /locus_tag="IVK51_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP6430075.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(50653..52428)
                     /locus_tag="IVK51_000455"
     CDS             complement(50653..52428)
                     /locus_tag="IVK51_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP6430076.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(52639..53541)
                     /locus_tag="IVK51_000456"
     CDS             complement(52639..53541)
                     /locus_tag="IVK51_000456"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP6430077.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            53820..56165
                     /locus_tag="IVK51_000457"
     CDS             53820..56165
                     /locus_tag="IVK51_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6430078.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
BASE COUNT        16683 a        11715 c         9118 g        18780 t
ORIGIN      
        1 aattctaacg ggattctgcg tcgtaatgga ctgccgaaat caatagattt tagagaagtg
       61 aatcagacat ttatttccag tgtcagcaat caacgcaatc atattccaag aaaatcattg
      121 aattacagaa caccaatcga aatatttttg agctatgtac aagaagcatt ttattctagc
      181 ttaatttgac aaatcatata atttaaaatt aacattgatt ctcctatgaa aatatctttt
      241 acctttctcc tgtgaaaagg aaatttcctt ttttatagtg ttattgatat aatcagaata
      301 ttcatcaaac tctgtatctg gagttaaaaa tctacagtaa catctataag ctttgctagt
      361 aatcttcttg ggttctctct ccattgtcaa gcaacatcaa aacacctact tactattcga
      421 ttcttttctg ccaaaaacga gtcagcatca ctcacaactt atgtgttgct gactccgact
      481 agtcctttgt tcaatatcaa cttattaaac agatataata tttttcttta tttcgtatac
      541 tccaagactt ccatctttct ttcatcttat ctctgaaaaa aacacagcaa agtgccctta
      601 ataaatatac attagaattt gcaaattttt tcatcttaag atatatccta ttcatattct
      661 ttacaatcct aatgggtgat tacaaatcta tttagttcaa gttctacatt ataaacacct
      721 ttcttgatag tatcatctgc tgcacaagcg atgtcgatcc acttattagg ggatttacgt
      781 gctcaaaagt ggctattcct tttgggaagt ccatccataa taatggtcca tggatttttt
      841 tagggtcttg tcgttcaaat ttctcaaaaa ctttattata cttccatagt tttataatta
      901 tttggtttca ctttggctcg ttcaaacacc aacttgtatt tcttttcaac gttcgaactg
      961 gagccccccc tataatctca ttattatttt catgactttt tactaccgtg gaattacaag
     1021 caacaacaat attatctgct aaatgaacac ctttctatat cacgcttttt gctccaatcc
     1081 aacaatgttt acctataact attttattat ttttattcaa aatcctatga gtattcaatt
     1141 caaaaatcgt atgtgaatcg gtatccataa ataatgtatc ccacgatact gtacagccat
     1201 ctttgaattg aatttcctta aagcaaatta tactcatttt tcctgtcgaa ttaaactttg
     1261 acccaatatc taaaactgca tttttcctta tgtcaatatg agccccttgt gcgataaacg
     1321 ctggcccttg tattcttagt attccttcaa tacttataat cgtttcttca tttcccgcag
     1381 tatttaatac tggaaagccc aaataaacta tttttccaga ttgtaatact atctcaattt
     1441 tccctctcga tttgcgtgta atccgtactt tacgatgcac caaaataggt aatctccacg
     1501 catctgaaat atgaagaaat ttataattga agtatatcgt tttaggtaaa tttaatatat
     1561 tctttattat caatccgagt ctcatctttc accatctctt tttgtaatca aaattagttt
     1621 ataaaggatt acacccaata taccgcccaa cgtattatat acaaaatccg aaatttgaaa
     1681 cgttcctagc ctcaaaaaaa gttgtaaaaa ttcaattatt gctgaaaaat aaaatgcaat
     1741 ttttcctcca tatactattg aggaccatat tttgggtttc tttatatttt catatttaat
     1801 agacattacc atataaacat atggaataaa aagaataata ttttctaaac acgctgtaga
     1861 taactctttt gttccagttg ttgtattata cttataaatc caccagtctc ccataacatc
     1921 agataaagga tttaaccaaa ttgttcgatt gattaatgtt gtaaataaaa tcatcgcagt
     1981 atagaatatc aaagcacaaa cttttctata ttttctatcc ttctttaaat gaagataaag
     2041 ttccattagt atacgtttat agccctcacc tgcagcaatg ccatctgttg catacataaa
     2101 atagaccagt cctaaaattg tcaataaaac agcaaaccca aagggttcat ataatttata
     2161 taatatatct gtaataattt ctgcaatcac tattatcata attatttctc caatatcaag
     2221 tatacactta tcgcttccaa ttacacatga tcatattaga gtgtattatc acattctcta
     2281 atattcaatt tatttttcct aaaatattcg aaataatcgt cttgataacg agtaagtatt
     2341 tttatacgat tgataacata gtaacaatat ttttaattat agtaaaaaat catctgcctt
     2401 tttcatccac ctaatcttat ttttcatttt attcaacatc cttaaataaa cttccgttcc
     2461 taagctctat aatatcttct taaaaaataa tattatggtg tcgttttcaa actcgtaatc
     2521 ttttacattg gaaaaataca tacttttctc aatgtagcaa ctcctatctc acagtattca
     2581 aaaattcttc aatattatga aatacttgtg catgttcttt aaataccttg tcagtctgtt
     2641 tataatcccc tactaaattt tgcattgctc ccaactcttc gacaaattgt catctccatc
     2701 tgtaaacagc cctaaaaact catataatgc tttttcatca atatttttcc catattattc
     2761 tcggtatagt ttcacaagtt ttacggattc ttcatattgt ttatcttttt ataatattca
     2821 tctagggaca taattacttt tgctagattt cctgcaacaa cacaatcgtc tgatacgtcc
     2881 ttgtttacta aactatttgc acctataact acattatttc ctatatgtac accctttagt
     2941 attctagact tcattcctat aaaaacatta ttacctattt tgatcctctc cgatgatccc
     3001 aaagcttctc catactactt ttaaaaccga tcaatcataa acatgagtca aaattgtaac
     3061 tccttcatat catctccaat ttcaacaaga gtcttcgttg gaacaaaaac tatacatcgt
     3121 tcgcctattc ggcagcctaa aaattgtagt tactttatat atcattctga tgtctcttct
     3181 catcttttta ccaataccga cgtaagaact ttcaaaatat acttaaactt cttattacgg
     3241 atattaaaca gaagaataac taaaatcgat atgacaaccg atataactaa atttatcact
     3301 aaacttatca ctttgaaatt tccgatatta acatacgaac aagcaaaata acttaaataa
     3361 gaagcaacaa aagctattac acacaaactt atgagcttcc aaacatattt acctgccgat
     3421 tcaaatttaa atatattttg gaaaacatta tataataacc agggtaaatc aaccaacaat
     3481 actgaaacaa tggttgccgc caaaactcca tataatccga accaattaac gaggatgata
     3541 tttaatacaa tgtttatcat tgctgtaatt aatggtctaa acctgtctgc gtaccatatt
     3601 ccagcagcat ctttgtataa cccaattaac tgatcaattt catataagaa aaaatgaact
     3661 atcataaata taacaaatga aatactcata agattatcac ttcccaccca gataatcatg
     3721 aacggctgaa ataatgtaat aaagcatgca caactaattg ccagaaacca acctataatt
     3781 gttgtaatat cacaaaaatc tatataattt ttttcttgac tttctacaat aagactgtta
     3841 cctatacctg ctatacatga ttcataaatt attgcaaata tactcattat ggcagaaata
     3901 ataaagtaat aattctgata aatcgcaagt actgaaagac ctaagaaaga tgaaataata
     3961 attgaatctg aagatcgtaa tattgtaccg cctaccttgt ttgtaactaa tccttttact
     4021 ttttttataa tatcgtcaat agtttctttc tctaactcac caacaggagt ataatcagga
     4081 tacatctttt gagaaacaac agcacacatt aagttaagaa ctatttgtcc caaaatagaa
     4141 gccaataaat acaaatagta attttttaag aaaatcagaa catatgcctg cattacaaat
     4201 tggaatgtaa aagtaattag catagacacg ttaattacat catttctttg atgtgcatat
     4261 attagactct ttctatatga aaataaccaa tacgacaaaa cagtatttat caaatacatt
     4321 aaatacaaaa ctgtcatatt taaatttcca ggaatcgaac ctttaactaa aaatctcaaa
     4381 aaaggaatgc ataacaatcc taatgtaaac actatccatc caataattga aaagtacttt
     4441 ttatatgcgt tcagtaaagc acaaatctct tttctattgt cttctgctat aggtttatac
     4501 atacttacag ttagtgcagc acctattccc aattccgcta aatttaacat ttgaaatata
     4561 gatgtgaaca ggttgttcaa tccagcatac tccattccca tctgataaat cataagtgta
     4621 cgcataaaaa agggaatgat aatctgatat aacttaaaaa ttgttccagt aattatattc
     4681 cttttagcat tttgtttacg attaattatc atatcatatg gctccaaact ttctattttt
     4741 tattgttacc taatactata taatttctaa aatcttgaaa tgtaataggt ttcttctagc
     4801 tctttgatta attacgctaa ttttcatgtt tggacaacca caaaaaacta tacattttca
     4861 atcaaagcaa cccattgttt tgtaatttct ttcaaattaa atttttgtac attattatat
     4921 gcgttcagtg acattctcat tgctaaatca gtattcttag taaacattat catgctctta
     4981 gctaattggt ctatattctt atcttcaacc aacatcccat taaaattatg atgtaccaaa
     5041 tctctcggac cagttttaca atcataagaa ataactggta atccataaga ctgtgcttct
     5101 aatataacca taggaaatcc ttcataaaca gaactaagca cataacaaga tgcagattta
     5161 taatattcct ccacatggtc tgaatgccct gctatagtta ctctttgcag atttaaatct
     5221 tttatctgct ggcgaagatc tttttctaat tcaccttttc ctacaatgat aagatcccaa
     5281 tcatctactt ttttttctac aattttccaa gcacttatca tgtcagcaaa atttttctct
     5341 tccactaatc gccctactgc aagaaatctc tttttatcat aatgatattc gtactcgata
     5401 ttagatatac acggatttgg catagttata acaaaatctt ctgataaact atattcatca
     5461 atatgttttt gacgatcttc ttctgtgaga accacaagcc tctttgcata tttttttatc
     5521 aattttttag ccttgaatct cttcttttct ttcagcattt ccattgtcca gtaattaaaa
     5581 tgttcccaag aaatcaattt gcactttgtt cctttaattg cccttgtagt atacaaagac
     5641 aacaaagtat caatatcaat tactatatca aattcatttt tgcatatata cttgtgcaat
     5701 ttccaaaccg gatataaata agttctatat aattttccct ccgttttagg atttaacata
     5761 taatcaacct taatcgtctt atctaaccca aaataaggct ctccactatt ccaaaagctt
     5821 aaaatagtga catcataacc taaatcagac aagctattgg caataatact ccctactctt
     5881 tcagttcccc cagaacgaga aattacacca ctaatgaaac aaattttctt caaagtactt
     5941 ttctccttta taagtgtaaa aattttttca gtgcaagtaa cctgttaaat tttttttgtc
     6001 caattgttga ttttaatcta taaacaaatt tttgtttagg tgttttccaa gatgcattat
     6061 attgatgaat gcttattgta ttactagttt tataaatttt tccagtatca taatccttgg
     6121 cagcaaaata atccgtagga tagatatgca ggccatttat tatttgttcg ctgttatcgc
     6181 gaaccaatcc aatagattca agataatctg taatataata cggactcggt ttcaagttca
     6241 atgtaccatc attttgataa aaacttaatg aatcgtaaat atcccttaaa cctttaataa
     6301 cagaatgtcc ttcttctgca ccaaaaccaa taccgccatt aacccagatt tccccttcaa
     6361 atcccatata ggcatgattt tcacgaagta tatctaacga cttaattagc tcaacatctg
     6421 tatctaaata aaatccacca tgattataaa taatatcaaa tcttgcataa tctggaacaa
     6481 aaccccattt acgttcttta tatgcatcgg ccatataacg gttcttattt aaatcataat
     6541 ttgattcatt ccattcaata atctcataat cagggcaata ctttttccag ctttctatgc
     6601 actttctatc ttttttcgga agtggatttc ccccgaacca acagtaatgt atcttcttag
     6661 gaatcataat atctctcctt ttgtattttt ttcactataa taatgctctt tccttttcac
     6721 aacttcaggc atggctaaaa tcatataaat cagtggcccc gttgtttcac ccctaagtgt
     6781 tgtgttatac acaaaaaaga ataccgacat aattgcaata actttaacaa gacttgcatc
     6841 tttatccttc ataaaagatc tttttgcatt gaaaaatgtc tttattataa aaaataggaa
     6901 tatcgaaact cctaatattc caacttctat aaataaccaa atatgatgaa aatactgata
     6961 atgaagatta ccaaacgatt tataaaactc actgacaaaa aaattggtaa actctgcatt
     7021 gccaatgcct actcccatta ggactaatct aaaattatta ttaaatattt catctactac
     7081 tctagagata ccagttattc gatttaaatc accaacacca tcatatccag aatctcttga
     7141 cgcatattct aaaagacctt gaattgtgaa attatcagca tagctttgtc cattatcgtt
     7201 caatgtaata aatattgtta taaaaaagga taatactatc gacacaagta ttacgcatga
     7261 taatatcttg atcactactt taaaatcaac ttttaaagtg aatactattg caacaaatat
     7321 tataaataat tcaggaataa aaactttcaa ttcagctaat gcagacataa aaagtgctgc
     7381 acaaacacat aaaataaaat ggatgaaatt tttttctccc ttgaaatatc cgattgtaac
     7441 aacagtaact gtcacacaca aaattaaatt caatggcata ttacaacctg aataattcca
     7501 aaaaactcca cctatcatat ctcctacaat tgttccagtt ggataggtga ccatgaatct
     7561 ttcaattaaa caaagtggca gtgaaatcca gaataatttt tcaattatag cgaatacttt
     7621 atagtaatct ttaattgtta aatatgttgc acaatttata tatactatga acatacgagt
     7681 gttatttcgt atcccccaga taatcaatcc tatattttct aaattaataa ctgagctaca
     7741 aatagaaact agtaaaaaca aaactatcag catctcattt ttgctagcat atattccttt
     7801 tctgcgtcta aagctaagtg catttaataa cacaaagata ttaattatat ctcccaaata
     7861 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
     7921 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
     7981 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
     8041 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
     8101 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
     8161 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
     8221 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
     8281 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
     8341 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
     8401 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
     8461 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
     8521 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
     8581 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
     8641 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
     8701 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
     8761 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
     8821 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     8881 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     8941 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     9001 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     9061 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     9121 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     9181 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     9241 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     9301 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     9361 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     9421 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     9481 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     9541 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     9601 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     9661 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     9721 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     9781 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     9841 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     9901 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     9961 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
    10021 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
    10081 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
    10141 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
    10201 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
    10261 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
    10321 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
    10381 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
    10441 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
    10501 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
    10561 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
    10621 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
    10681 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
    10741 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
    10801 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
    10861 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
    10921 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
    10981 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
    11041 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
    11101 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
    11161 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
    11221 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
    11281 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
    11341 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
    11401 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
    11461 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
    11521 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
    11581 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
    11641 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
    11701 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
    11761 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
    11821 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
    11881 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
    11941 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
    12001 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
    12061 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
    12121 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
    12181 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
    12241 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
    12301 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
    12361 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
    12421 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
    12481 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
    12541 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
    12601 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
    12661 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
    12721 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
    12781 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
    12841 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
    12901 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
    12961 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
    13021 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
    13081 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
    13141 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
    13201 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
    13261 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
    13321 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
    13381 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
    13441 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
    13501 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
    13561 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
    13621 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
    13681 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
    13741 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
    13801 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
    13861 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
    13921 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
    13981 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
    14041 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
    14101 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
    14161 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
    14221 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
    14281 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
    14341 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttgatagc
    14401 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
    14461 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
    14521 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
    14581 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
    14641 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
    14701 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
    14761 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
    14821 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
    14881 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
    14941 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
    15001 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
    15061 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
    15121 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
    15181 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
    15241 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
    15301 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
    15361 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
    15421 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
    15481 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
    15541 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
    15601 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
    15661 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
    15721 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
    15781 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
    15841 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
    15901 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
    15961 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
    16021 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
    16081 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
    16141 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
    16201 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
    16261 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
    16321 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
    16381 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
    16441 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
    16501 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
    16561 tctaattcgg cttgtagcga agcgactaga gaaatgactt gtgatttttc attgctgtat
    16621 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
    16681 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
    16741 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
    16801 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
    16861 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
    16921 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
    16981 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
    17041 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
    17101 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
    17161 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
    17221 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
    17281 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
    17341 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
    17401 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
    17461 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
    17521 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
    17581 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
    17641 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
    17701 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
    17761 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
    17821 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    17881 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    17941 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    18001 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    18061 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    18121 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    18181 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    18241 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    18301 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    18361 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    18421 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    18481 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    18541 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    18601 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    18661 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    18721 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    18781 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    18841 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    18901 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    18961 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    19021 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    19081 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    19141 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    19201 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    19261 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    19321 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    19381 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    19441 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    19501 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    19561 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    19621 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    19681 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    19741 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    19801 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    19861 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    19921 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    19981 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    20041 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    20101 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    20161 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    20221 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    20281 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    20341 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    20401 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    20461 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    20521 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    20581 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    20641 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    20701 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    20761 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    20821 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    20881 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    20941 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    21001 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    21061 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    21121 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    21181 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    21241 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    21301 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    21361 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    21421 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    21481 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    21541 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    21601 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    21661 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    21721 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    21781 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    21841 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    21901 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    21961 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    22021 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    22081 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    22141 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    22201 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    22261 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    22321 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    22381 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    22441 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    22501 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    22561 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    22621 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    22681 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    22741 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    22801 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    22861 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    22921 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    22981 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    23041 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    23101 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    23161 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    23221 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    23281 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    23341 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    23401 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    23461 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    23521 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    23581 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    23641 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    23701 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    23761 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    23821 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    23881 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    23941 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    24001 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    24061 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    24121 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    24181 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    24241 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    24301 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    24361 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    24421 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    24481 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    24541 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    24601 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    24661 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    24721 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    24781 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    24841 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    24901 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    24961 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    25021 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    25081 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    25141 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    25201 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    25261 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    25321 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    25381 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    25441 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    25501 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    25561 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    25621 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    25681 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    25741 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    25801 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    25861 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    25921 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    25981 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    26041 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    26101 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    26161 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    26221 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    26281 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    26341 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    26401 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    26461 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    26521 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    26581 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    26641 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    26701 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    26761 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    26821 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    26881 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    26941 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    27001 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    27061 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    27121 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    27181 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    27241 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    27301 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    27361 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    27421 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    27481 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    27541 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    27601 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    27661 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    27721 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    27781 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    27841 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    27901 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    27961 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    28021 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    28081 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    28141 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    28201 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    28261 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    28321 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    28381 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    28441 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    28501 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    28561 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    28621 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    28681 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    28741 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    28801 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    28861 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    28921 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    28981 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    29041 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    29101 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    29161 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    29221 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    29281 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    29341 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    29401 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    29461 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    29521 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    29581 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    29641 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    29701 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    29761 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    29821 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    29881 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    29941 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    30001 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    30061 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    30121 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    30181 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    30241 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    30301 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    30361 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    30421 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    30481 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    30541 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    30601 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    30661 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    30721 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    30781 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    30841 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    30901 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    30961 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    31021 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    31081 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    31141 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    31201 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    31261 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    31321 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    31381 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    31441 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    31501 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    31561 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    31621 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    31681 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    31741 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    31801 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    31861 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    31921 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    31981 tgattgtccg cagtttaaat gataaaatta agattgtaat agttctttca caatggcatt
    32041 tacttggttt ccatctgctt ttcctttgac tttaggcatg atcgcaccca tgactttgcc
    32101 aaattctttc attgatgaag caccagtttg tgtgattgct tcttgaacaa gttgtcgaat
    32161 ttcttcgtcg gaaagttgtt ccggcatgta tttttcgact atcacaattt cactttttac
    32221 tttttcagct aaatcgtcac gtcctgcttc ttcaaactca tgcaaagaat cacgtcgctg
    32281 tttcatttcg cgagacaaca ccgttaattc ttcttcttct gtcaagtcgt gaccttcttt
    32341 aatctgctcg ttttgtatag atgacttgat cattcggatg acttgtaaag attccttatc
    32401 tttcgctttc atcgctgttt tcatgtcatc gtttaatcta cttagtagtg acacactacc
    32461 aactccaatt catcacagaa ctagaatttt ttgcgttttc tagctgcttc agattttttc
    32521 ttgcgtctta cacttggttt ttcgtagaat tcacgtttac gagattcttg taaagtaccc
    32581 gcttttgaca cggaacgttt gaagcgacga agagcatcgt caagtgattc gtttttgcga
    32641 acgactgttt ttgacatgtt aattccctcc ctccgagctt aaaaagtaac cgtaattgtt
    32701 attgaaatat atctcaaaaa acaagactgt cctgtaaata ttatatctaa tgctgttttg
    32761 accgtcaagg aaatcttgct tttttttaaa attatcaaat gttattttat ttaaatcctt
    32821 attgacaatt ttcacatctt ccgaaaatct cgaatctatg cccttcgatc gtacatccac
    32881 cgagctgttc ttcgaaataa ttcattggac acatatggat ttctttcgtt gttccgcaca
    32941 ccgtacagat aaagtgatgg tgatggccga tttccccttg acagcaatga aaacgaaact
    33001 tcatctctcc atttaaatcg gtttcttcca gtaaatcgat ttctgcaaaa tcatggagat
    33061 ttcgataaat cgtatcgtaa cttactcctt tgaattcctg attcatatag tcaaaaactt
    33121 ctttagcgga tacgtagcga ttgcgcttca ctaaataagt taacaatgct tctctttttt
    33181 tagtatattt aaaaccgtta tttttcaatg tttctagtga ttgttcaatc aatgtctgtt
    33241 ttttcatgac ttattcctcc aaatcaaaat gattacgaag tacacattct agagtatggt
    33301 ataataaaca taaaaaaaat gcaagtgagg ttttatccat gtcttccgat tctacagaaa
    33361 tcattacttt ttttgtcatc tctgactctg ctggagaaac tgcgacaaaa ttagcgcaag
    33421 ccactatggc ccaatatcct accgttgagt tcaatttatt ccgcagaaca tttgtgacag
    33481 atgagaaaac attgatgcag gcactgcaag atgcactaaa agaaaaagcc cttgtcctac
    33541 acacattgat caatcaagaa ttgatcgaga tgacgcgttc tttttgtgaa gaacatcaat
    33601 tatttagttt tgatgtgatg actccgccga tatcagaaat cgagcgctta actggtatca
    33661 agcccattcg gcaacctggc gcccttcatt tattgaatga gaactatttc aaacggatca
    33721 aagcaatgga atttgctgta aaatacgatg acgggaaaga tcctcgtggg tttctagaag
    33781 cagatgtcgt tttgctaggc gtatccagaa cttccaaaac gcctttgagc ttgtttttag
    33841 ctaacaaaaa tttaaaggtt gccaatctac ctttgatccc acaagcacat attcccaaac
    33901 agctttggga gatcgatcct aaaaaaatcg tgggactgac gaataaccca gatattttaa
    33961 acaatatccg taaagaacgg atgcgctctt acggattaaa tcctgatact gcttattccg
    34021 atatcgaaaa aatacgagct gaactcgatt ttgcgaatga tctttatgaa aaattgggct
    34081 gcgtagtaat caatgtcgct tctttatcta tcgaagaaac agcttcactt attttgaatg
    34141 ctttagacct agaagatcat agttattatg gaactgaaac caccgaagaa acagactaac
    34201 agtttttctt gtctttttct taaaaaaact ggactgtgtc agcgaagaag attcgttgat
    34261 acagtccagt ttgtctctat ttgcacctat cgttattgat gttccacatg tttgagcgtc
    34321 tggttcaaaa tatccagtac gtgagaatca tcaagactat acaaaatcgc ttttccctca
    34381 cgatgggact tgacgacatg gttttctctt aataaacgca gttgatgcga tacagcagat
    34441 tgttccattt ctactgcttg ggtgattgct gtcacatttt gttccccgtc tttcagatac
    34501 agcagaattt tgagacgagt ggggtcactc aacattttga atagttggct gactttctct
    34561 acttttgttt tttgcgtgtc tggcaatcca ttcacctcca acttgaaaaa agactgactt
    34621 ctaaagttta ccactttttc catcagtctc aagttttttt attcttctgt catttttttg
    34681 ataatcgctt cataagcggc tactttttca gctgcgtctt ctgaagagtt tccttttaca
    34741 ccgatataaa atttgatttt cggctcagtt cctgaaggac ggatagcgat ccagccattt
    34801 tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg ttagtttttc tacgtttcct
    34861 tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt ggatgacttt tgctccggca
    34921 aattctgtcg gtgcttcttc acggaatttt tccatcaaag cagagatttt tgcagagcct
    34981 tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt agtagccata tgtttcaaaa
    35041 atcgtttgca agccgtcata caatgttttg ccttgttttt tatagtaagc tgctacttct
    35101 gctaataaga gcaatgcttg aatcgcgtct ttgtcgcgaa cgaaaggttt aactaaataa
    35161 ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat gatcttcttc aaattgttga
    35221 attttttcag caatgaattt gaatcctgtc aatacattga acatggtagc cccatagtgt
    35281 tccgcaattg ctgtaggcaa ttcgctagaa acgattgatt tcaataccgc tgcattttca
    35341 ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt gagcaaacag ggctccgatt
    35401 tggtttcctg tcaatacttc ataggagcca tttggcaaac gaacggctgc gcctaaacgg
    35461 tctgcatcag gatccgttgc aatcagtaag tcagcgcctt ctttctcgcc aagtcgcatc
    35521 gcatactcga aagcagagtg ttcttctgga ttcggtgatt tgactgtagt gaaatccggg
    35581 tctgcgacag cttgttctgg aactagtacg aattgttcga atccagcttg tttcaacgca
    35641 cgttcgccca acatttttcc tgttccgtga agtggtgtgt acacaagctt caaatcttta
    35701 cccattgttt ccaccaattc atgatcaatc gttactgctt tgatttcttc tagataagcg
    35761 gcatccactt cttctccgat gatcgtgatc aatccgctat gttcagcttc gtcgtcagat
    35821 aatacttcaa cttttaacgg attgctgact tcacggacat atttcgtcaa tgcatctgcg
    35881 tctgcaggag gcatttgtcc cccatcttct ccgtagactt tatatccgtt gtaagctgct
    35941 ggattgtggg atgcagttac catgattccc gcaaacgtct tcaaatgacg gactgcaaaa
    36001 gaaagctctg gtgttggacg taaactttcg aatacataag aagggatatt gtgatgtgct
    36061 aatgttttgg ctgcttccat ggcaaattct ggtgattgat ggcgtgagtc ataagcgata
    36121 gcgacacctc ggcgttttgt ttcttcacct tgtgtatcca tgaaacgtgc aagtccttct
    36181 gttgcttgtc ttaccgtata gatgttcatt cgattgatcc caggtcctaa gattccccgc
    36241 atgcctgcag taccgaattc caacggtgca taaaaagcat cttcaagttt ttcaggatct
    36301 tcacccagat catttaattg tttttttaag ttttcttcta aattttcttc atgtacccat
    36361 tgttcataca cttgttccca agacatgaaa ataacacctc tttctgaaat atggttctta
    36421 ataagtatag cattcactta ttagatatga aagtatgagg accaaaaaac gcttaaatca
    36481 agaaaagatt cttttatcaa gcacggttac aagtattcgc ataaatcgga ttcagtagat
    36541 ttcacgcaaa aaaaagagta aaagaacaat tgaaaccgtt ctttcactct ttttaaactt
    36601 atgcagttgt ttcttctaaa gattccaagt atttatacac ttgttgtcca gcaatcccac
    36661 catcaccaac agcagtagtg atttgtcgca aatctttttc acgaacatct ccgatggcaa
    36721 aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg aatccagccg gcttcgttcg
    36781 tcaacccagc ttctctaaat ggtgcagtta atggatccaa tcctacatag atgaatactc
    36841 cgtctgctgc caaatcaaac tcattgccag ttttgacatt acgtgctttc acacctgtca
    36901 ctaccatttc atttccaacg atttcgtcaa cgactgtatc ccacataaag gaaatttttt
    36961 cattcgcaaa tgcacggtct tggatgatct tttgtgcacg taattcatct ctacggtgga
    37021 cgatgactac ttcagaagca aattgtgtca aatagatggc ttcttctact gctgagtctc
    37081 ctcctccgat cactactaga cgtttgttac ggaaaaatgc accatcacaa actgcacagt
    37141 aagaaacgcc acgtccagca aattcttcct cgccaggtac gcctaattta cggtggatac
    37201 agcctgttgc gataatgact gtttttcctt cgtaagtttt atcttcacaa ataacttctt
    37261 tgtggctgcc gtgatccttg atatcttgca cgattccata agcattttct gtcccaaatc
    37321 ttgaaacacc ttcatacatc ttataagcta gatcaggacc catgattgaa tcgaaaccag
    37381 gatagttttc gatttctgcc gtattgttca tttggccgcc aggagcgcct tgttcgatca
    37441 tcaatacgga taagtttgaa cgtgaagcat ataaagcagc agtcatccca gcaggccctg
    37501 cgccaataac gattacatca tacatatgta gattctcctt tacttaatca agtaagcata
    37561 ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg cgtttctcga atagctttat
    37621 tatactcgaa aaatccgctg aaaaacaata gacttttata aatcattgat caatcgttcg
    37681 aaagattttc tccatttgaa tcaattactt ttttatacca gttgaatgat ttcttcttcg
    37741 aacgggctaa tgtgccattt ccttcgttgt cacgatcgac ataaatgaat ccgtaccttt
    37801 tcttcatctc acctgtacca gctgatacta aatcaataca tccccaagtc gtatagccta
    37861 atagatcgac tccatctaat tctactgcat ctttcattgc ctggatatgt gctgccagat
    37921 agtcgattcg ataatcatct tccacataac cattctcatc gggcgtatcg actgcaccta
    37981 agccgttttc tacaataaat agtggttttt gataacgatc ataaatatca ttcatcgtta
    38041 ttctcaagcc taatggatca atttgccaac cccattcact tgcttccaga tatggatttt
    38101 tgacagaagc aaagatattt cctgcagtct gttccaagag ttcgttgtca gtagtcgaaa
    38161 ctcgagaaga ataataagaa aaggagataa aatcaacagt atgttctttc agcaatgcgg
    38221 catccccagg ttccattttg atctgaatgc catttcgttc catttctttc aatgcataag
    38281 cgggatattc tcctcgtgac tggacatcaa tgaagaaata attttctcga tctgactttc
    38341 tagaagccca aacatcttca ggattgcaag tgtatggata atatgctcct gctgccagca
    38401 tacagcctac tttgttctcc ggatctactt catgagcgat cttcgttgcg atggcacttg
    38461 caaccaactc atgatgtgcc gcttgatatt tgatttgttc ttcattttct ccttcttcaa
    38521 aatacagccc tgcgcccatg aatggtgcat gaaggatcat attgatctca ttgaatgtca
    38581 accaatattt gacaagtcct ttatagcgat tgaagatgac tcggcacaga ttttcataaa
    38641 aaccaacgag ttctctggaa cgccacgcac catattcttt gatcaaatgc atcggacaat
    38701 caaaatgtgt aatcgtcaca agcggctcga tcccgtactt ttgacattct ttaaagacat
    38761 cctcataaaa tttcaaccca gattcatttg gctctttttc atcgcccatt ggaaaaatac
    38821 gactccaagc aatggacatc cgataagtct tgaaccccat ttctgcaaat aaagcaatat
    38881 cttccttata atgatgatac atatcgatcg caatttttgc tggataaaaa tgattttcat
    38941 caaattcaaa cattttcttt tttccagtaa tgacaggaaa acggtcttct ccgattggca
    39001 caacatctac gttcgctaac ccacgtccgc cttcatcata accgccctcg cattgattgg
    39061 ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc catcatactt cctccattac
    39121 atgtgtattg cttgataatt ctcttgccag ttcatcttgg acttcgattc cttctgcttt
    39181 taaccggctg attgtttcct tgaattgcgg taaatactgc tgatgtgcaa taaacaactc
    39241 atccatgatc cgttttgctg tctttcctga tcgtataagc ggattgatcg taaacgcttg
    39301 caaagctgtt ccataatcac cagtcacggc tgcttcgatc gtcagcaatt ccatattctt
    39361 cattacttgc aaccagcctc tttcagcagc aggaagggaa ccaaacgcta catttcgtgc
    39421 gccttgtgcg ccaacatagg cagtgacttc tactacacaa tctgccggca gatcaggaac
    39481 agctccatca ttctttgtag ataccacgat ctgtgtttct ttatttccat agatcgaagc
    39541 aatcgtttca caggcagcat ctgaataata agcaccacct cgtttttgca attgttcagg
    39601 tttatgatcc aattctggat ctttgtataa ttcaaacagc tctgcttctg tttctttcac
    39661 ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa gcatgttcca gcatttcttc
    39721 ttcacgatag taatagcggt ggtagccaca agggatcatg ttcatttgtt ttaattgttc
    39781 tttgaaaaac gggatgtcaa agatattttt cggcaatcca ttgtcttcat cgtataaacg
    39841 atcaatcaat tctgccgtgc ggtcatttcc tttattatct gctactttgt gccaatggaa
    39901 gtgatttaga ccggcaaact tgtagatcag ctcttctttt ttcttgccaa gcattgttgg
    39961 ttctgtcatc atcgccatta ctggcacatt gcataatccg atcactttat cccatttgcc
    40021 atatctgata actgcttctg tcaccatccc gcttgggttc gcgaaattaa tcagccaagc
    40081 atttggacat aaacgcttca tgtcttcaac gattgccaaa accactggaa cggtacgaaa
    40141 agctttgaat atcccgccag caccatttgt ttcttgccca atcatcccat aataactagg
    40201 gatccgctcg tctttgattc gtgcatctaa ttgaccgaca cgaaactgag tcgtaacaaa
    40261 atccgcatct ttcaatgctt cttcccgatc cagagttgca tagattttga cttcatacgg
    40321 agaagcatcc cacatacgct gagacatctg actgacgatc tcaagctttt cttttccttc
    40381 ttctacatct acgagccaga tttctcgtat cggtaattcc tcatatcttt tgataaatcc
    40441 ttccatcaac tcgggggtgt aactgctgcc tccaccaatc gtagcgattt tgattccttt
    40501 tgccatgttc tcactccttt actttttaca cttttattat cttttttgta aacaaaacaa
    40561 acaatagaaa ttcgtttctt tccagtaaca tttacaaaag ttagtgtttt cttctggatt
    40621 tttgtaaata tcttgtcaaa aaatgtcttt attaagctat ataacataaa tccgtttaca
    40681 aatcattttc aaaaaagtta taatttacgt aaaaaaacaa gaagaaggta tctacatgtt
    40741 attacgtgaa aaaatcaaag aaatctcctt ttctccttca gaaaatgaag tgattcagtt
    40801 tctcttaagc gattctccag cacttggcga aatgaccatc caagagattg ctgagcgatg
    40861 ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa atgggttttg atggctgggt
    40921 cagttttcgt aatgcttttt tagctgaaac agcctatctt gaagggaatt ttcaagacgt
    40981 agatgcgaat cttccttttt ccaccacaga cggtattatg acgatcgcaa ataaaatggc
    41041 ttctttagaa cagatgacga ttcaagatac actttctctc attcatcatg accaactgca
    41101 aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg attttcgcca gtaactcgaa
    41161 tactttgatt tctcaagatt tcatgctgaa aatgaagcgc atcaaaatgg atgtcacgat
    41221 tgtcaccact tttggtgaat cgttctatga agcgtacaat tgcacaagtg atatgtgtgg
    41281 gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt cgtaccgcag aaatattgaa
    41341 acaaaatcat gtgccaattt tagctttgac cagtatcggt gattcaacgc tatcttcaat
    41401 gagtacagcc gctttgcgac ttacgacacg cgaacgactg tattcgaaaa ttggtaattt
    41461 tacgatcaat acatcgattt gttatcttct agacgtgtta tatagttgtg tttttgcaga
    41521 aaattatcaa cagaatttag tccatctaat ttctgtagga caaaaatttg ataaacgccc
    41581 tatcagctca gccatcatga aagagccttt caattttgaa aataaagaag tggactaaaa
    41641 agaatagtta agcctagtcc attctttcac ctaaccgttc aggtcagtgc ggtgataagt
    41701 gcatcgtctg gcccgaccgt ttccccttga ttttccaaaa tatccaaata atccatgtaa
    41761 ttgatgacaa tcacaggtgt ttctaaactg tatccttcct cttcgatttt ctttttgtcg
    41821 aagcgaagca acagatcgcc ttgtttcact ctttctcctt gctgcaccaa gctttcaaaa
    41881 tatttccctt ccaactgaac agtatccaat ccgacatgaa tcagcaattc cagacctgct
    41941 tcagaaacca tcccgattgc atgtttcgtc ggaaacaggg acatgactgt tccgtcaaaa
    42001 ggggcacgta cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt
    42061 gcaaaagctg catcctttgc tgcattcagt ggagcgattt gtccttgtat cggtgttttt
    42121 acaatttctt tacctgatct tttctgtcta tcatctgttt catctgcttc ctccttccaa
    42181 aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg cgatcccaat caatacatta
    42241 atcacatctg ttaaatcacc atctggtcta atatagttgg gaattgtaaa gatgcctagt
    42301 ccaccttgat tccaagaagt taaattcatt aacccactat acgcgccccc taacgcacca
    42361 ccgacacagg taaaaataaa tattttttta cgtggcaatg taaacccata aatagctggc
    42421 tctgtaatcc ccacaatacc agaaacaatg gctggtattg caattgcttt gtcttttgga
    42481 tttctaagct taaagtagag tgcggtaagt gctgcagttt gtccaaaaga cactactgca
    42541 acaggtgtca taatattttg ccaaccattc acagaaaatt gcatcaatcc cattggtacg
    42601 attgcccaat ggagaccaaa catcaccaga atttgccaga agaagcctac gattgctcca
    42661 taaataactg gcgaaagtgc taaaagtgtt tcaaatccag ccattaaaat atcagttgcg
    42721 aatgtgaata ctggtccaac gagtaaaaaa cctaaaggca ttgtaataag aacggtgaag
    42781 aatggaacga agaaattcgc gatcatctca ggtactaact ttttaattgg tttctgaatt
    42841 tggctagcta acaacacaat aaagataatg ggaatgacag aagaagcgta gttacgcgca
    42901 atccagggaa tgccgaatac tgtttgataa ataggtgctt caaaaatagt gccagaaaat
    42961 atggtcgtta gtggtgcttc tgctgcacca ctcaaagctt ccagcgacaa gctaggattc
    43021 attaatgctg cgccaagcaa catccctacg aattcattta ctttaaattt acgggcagct
    43081 gttaaaccga taataacagg cataaattgg aatacagaat caccgatatt atcaaacata
    43141 acaaatgtgc cagaaccgcg gtcaaacgcg cctaagaaga caaggaatgc tgtcagtcca
    43201 cgaatcatcc cgtcagcagc taggatagca aggaatggtt ggaaacagcc cgatattcca
    43261 tcaataagca tatcaaacgg tctcattttt tctgttgaaa cagcctcatc aaaagtaagg
    43321 tttcctgctt ttataacctc ttcaaacacg actggcacat gattcccgat gacaacttga
    43381 tactgccctc ccgctcgcat taccgttaca ataccgtcca tatttttcaa gatctcatca
    43441 tttgcttttt cttcgttttt taatttgaaa cgaagccttg tgatacaatg tgtcaaacca
    43501 ttgatatttt ccttcccgcc gacattttct acgatttttc tcgctaattc ttggtatttt
    43561 cccatttcta ttccctccaa tttattagtg gaaggtttag ccaactgaat gtcactaccc
    43621 ttatttcata cgttcttgtt aatttatcct ccttttttta tttatttaca acgattctca
    43681 tgcactaaac gcgcagtatg gatcgttaaa tacatttttt cgtccgtgga catctggtag
    43741 ttgaacgtct cggctaaaaa gtctgctatt ttctcaacgc aaccaaacgt atccttatat
    43801 tttttttgaa taacctgaaa caattcctca gaaacttctc ccacttctga tttttctcca
    43861 tctaggacgc gtgtcagaaa aaaacgtaag tgtgtgacga atcgaaaaaa atggactgaa
    43921 tcttcatcaa atgtaatctt atagtaatag cggacgatat ttagaatatc ctgtattgtt
    43981 atagtgaaca ggtaggtatt gtcttgttgg ctttgaatgc cttgtgcatt gactaaatgc
    44041 aatgcgataa atccggcttc atcttctgat aacttgatgt ggtatttatc cgagattttc
    44101 ttcactgttt tttttcccga tggcatactc tttaggaaat agttgtttga tatcatacag
    44161 caatacattt ggtacaaata ttcccatttt gtgacgttcg atagccgtat acaaatgatc
    44221 cgtcaatgca atatacacag aatcattcaa ctggacttgt agagcctctt ttgtatattc
    44281 aatgatttca caagaaattt ctaagtactc aagaggaatc gcttgtgcca attcttggaa
    44341 ctgttgtgaa agcccgtcat ttgtcaactg aaattttttg tcgatgcatg agtcaaaaat
    44401 cgggtcaccc actttctttt tgaatgccaa accttttccc atcacgatca tttcctgatt
    44461 tctttcatcc ctggttatta cgacgttgtt attcagtact ttctcaatca gcatgttttg
    44521 ctcctttcca tgatatatag ggctgtatcc aaaacaaaaa gaaaaaccca aaaagccgtc
    44581 catggaacca tgggagactc atcaggttta gcttgcatca ggcaatcact atccaagttg
    44641 tatcgttttc tttatattat cgaaagcaaa cgcttttgtc aacgctttct tagtggttat
    44701 tagccgcttt tgactagctg tcgtcgtctt ttttaaatcg ggtgactacg ccactaacgc
    44761 tcacagatga agaatctgcg aagctctgtt ttattttcac tctagcgctt agggcaaaat
    44821 gctactatgc tactgtcaca acctcttttt acacaccact gctttgcagt ttgtacatat
    44881 ctgcatacaa accattttgc gctagaagtg tttcatgatt cccacgctct actatatgac
    44941 ctttgtctaa aactaaaatc aattctgcat cccgaatcgt ggacagccga tgggcaatcg
    45001 ctatcgttgt ccgtccttgt cgcatttttg ccaatccttc ttggatcaat ccttcggttt
    45061 cagtatcgat atttgctgtt gcttcatcca atactagtat tttaggatct gtcacgattg
    45121 ttcgcgcaaa agtgatcagc tgtcgttgtc cactagaata gctggcaccg tgttcgatca
    45181 cttttgagtg atatcctttt ggtaattcat cgatgaactt gtttgcttga acaaactcag
    45241 ctgcatggcg aatctgttcg tctgtgatag ctggattcat catccggata tttcctgcaa
    45301 tatctccata aaacaagaag gcatcttgca agaccaatcc cattttctta cgtaactctt
    45361 ccattggata ttctcgaatg tcatgatcat caatcaagat ttggccttca taaaattcgt
    45421 aaaaccgcat caatacgttg atgatcgagc tttttccgct tccggtgtgc cccactaatg
    45481 caacggtctc acccggatta gcgacaaaag agatattgtt caatacattg tgctctccat
    45541 cataagaaaa gcttacattg cgaaactcga tcttacctga agtaatcgct tgatctgctc
    45601 caggattttg ttgcggtgca tattcttctg tatccatgat tttcaaaata cgactaccgg
    45661 caaccatccc atctgtaaag gtgctcaaaa agtccatcat ttgagacatt ggattaaaaa
    45721 attcctggac ataggtcgta aatgcataga tcagtccaac ttctacggga gaatgcaacg
    45781 catcgatccc aaacaaagtc aacgccaacg caatcgccaa tgcatagaga aaattgatga
    45841 ttggacttaa cagaatcgaa ttcgttttga tcatcgcata gcgcgtgtct aaatactcgt
    45901 tgttcacttg ttcaaattct ttagctaaac gagcttcttg gcgaaactgc tggataatac
    45961 gcatgccgga aatcgattca ttcaatttcg tgttcagctg acttaatttt tcccgcatat
    46021 ttcggtaaat acgagaactg aatttctggt aataccagat gacgattcct aaaatcggca
    46081 agaacaccaa agtccacaag gcaatttctc cattgatttg aaacatggca acgaaagaag
    46141 aaacgactgc aaagatccca gtcaatacca tcaaaaagac ataccaaaat tcaaatagcg
    46201 tttctgtgtc attcgtcact cgagaaacgg tcgatcctgc cggtgtctgg tcaaaataac
    46261 gcatacctag tgtatgcagc ttttcaaata atcttacccg aacatactga taggtcttca
    46321 atgaagccat ggaatacaaa taccattgga aaaaccaaat gatacttttg actatgactc
    46381 caaacaagta cacaccagca aaaaacaaaa taacttgtgt cgtagctgac tgtttagcca
    46441 gatgatcatc cataaaagtt tggatgatcc ttggcaatag gacattgatc acagacaggg
    46501 caaacgcaaa taagatagca acgatgaacg ttcttctaaa aggtttggcg aacttcatca
    46561 gtcgtttgat gatcttcttc tgttctttaa gtggaatcga ttttgtccat tccgattgat
    46621 aattttcatc catcaggcct cgcctccttc tatcttcgct tctaattgct gtttttccca
    46681 cattcgttca taccagccac ctaatgcaag cagctcgtgg tgggttcctc gttcaatgat
    46741 tttcccttga tcgacaacaa tgatttcttt tgcatgcatc acgctgctta aacggtgagc
    46801 tgcaatgatg gttgttttct cttttcttat ttcttttaaa ttatgcaaga tagcttcttc
    46861 tgttttggca tctactgcag aaagtgcatc atctaagatc agcaattcag gatttttgat
    46921 caatgctcga gcaatcgaaa tcctctgttt ctgtccgcca gataaagaga ctccacgttc
    46981 tccaactaag gtatcataac cttttggcaa ttccttgatt tcattgttga taaatgctaa
    47041 agaagccgca tgctctactt cttcttctga aagttttgga ttagaaaaac ggatattatc
    47101 tcgaatggtc atcgaaaaca ggaagtgatc ctgaggcaca taaccgattg atcctaataa
    47161 agcattcaat gaatattctt tgatcgaacg tccgccaaat tggattttcc cttgataatc
    47221 atcgtattca cgcatcagta atttgatgat tgtcgttttt cccgcaccag ttttcccaac
    47281 aatacctaat gtttctcctt cttggatcgc aaatttgatt tgttctaatg ctggtgattc
    47341 ttctttcgga tagttaaaag aggaaatagc tacattcagc tcgccttttg ccggggtctg
    47401 cactgcaccc agttgttcca cgatatgcgt cttttcatgc aaaagttcat tgacgcgatc
    47461 ataagaagca tttcctcttt ccaatacgtt gaacaatcga ccaatcgcaa acatcggcca
    47521 aacaagcata ccgatatagc taataaatga gaccatctgc ccgattgaga tcgtgccttc
    47581 caagacatac tttccaccta aaataattgt tgcaacataa gacagaccga taatcaaggt
    47641 aatgaatgga tcaaataagg agtctaaaaa attgactcgt ttgtttttaa taattgcatc
    47701 ttcaatcttt tcttcaaaat ccgcgagatc ttctttttct tgtccaaatg ttttaatgac
    47761 tttgatccca gtgatacttt cttgtgtctt atcattgata ctagaaaaag cttcttgcga
    47821 atcacgaaaa gcatcgtgga gttttgaccc cagcactcgt gaagtaacgg ctagcaaagg
    47881 cagaggaatc agcgcaatca gtgttaagcg ccaatcaata aacaaaatca tcgcaatgat
    47941 cgttgttccg cccgtaatca ctgaatctgc aaaggtcaag attccagctc ctgctacatt
    48001 ctggattgca ttcaaatcat tcgttgcatg agccatcaaa tctccagtcc gatatttttg
    48061 gtaaaagaga ttgtccattt ctgtaaaatg tttgaatagc tgctgccgaa gttctctctc
    48121 caaccgagct gcacttcccc aaatattcgt ccgccagata taacgaaaaa gatattgtaa
    48181 gaaagctgca aggatcaata ataagatcca ccaggaaatg atctttattg ataaattctt
    48241 atcggcgatc tcatcaatga tgatcccaat cacttttgga ggaactaact gtaccaaggc
    48301 cactgcgaat agggagaaaa tcccaattaa ataatgtttc ttttcctgac gaaaaaacca
    48361 acctaatttt ctaaaaatcg acattttact cctcctttgt ctttatcaag agcataggat
    48421 tgtctgtgct tgtctttttt gctgaatagt ctgaatagtc taacaaatta cgaaataaaa
    48481 aacggactga aaaaatcagc ccgcaaaaag tctttgggtc agaacctatt ttttcccttg
    48541 gttcttcatt tgctgcatca tctggcgaat tttcttctca gatggttttt gccccattga
    48601 catcatcatt gatcgcaaca tgtcctcatt aacgggagga tttttttcta aatagtcttt
    48661 catatattta cgcgctagga aaaagccacc tactgcgccg attagcaaag caataatagc
    48721 aataagtacg acaatacctg ttgataccat ataaatctct cctttctatt tccgaattgc
    48781 tctcaatgat ttattttact aaagtttatt gaaaaaagaa aggcgtattc tttagtttca
    48841 tgcaaaaaat agtggttttt ttagagaaga acaaaatttc tttcattaac cagcaagaca
    48901 caccacgaaa aaaaattagg cataccgaat ctttttcttt aattttttct ttctgaggtg
    48961 tagaatgtaa tcgtagataa ataggaaagg tggttttttc ttgaataatc aatccaacaa
    49021 acaatttcat aagcatcgac ttattgaaca tacaaatggt ttatctttag ctgaagtaaa
    49081 tggaacagtg gatgtaccaa agggaaaaag tttttttaaa acacttcttg cttactctgg
    49141 accaggtgca ttagtagcag ttggttatat ggatccaggt aactggtcca cttcgatcac
    49201 tggtggacaa aatttccaat atttattgat gtcagttatt ttgatgtcta gtctaattgc
    49261 tatgctttta cagtatatgg cagcaaaatt aggaatcgtg acccaaatgg atttggccca
    49321 agcaatccga gcacggacaa gtaagacatt gggtgtcatt ttatggatct tgactgaatt
    49381 agccattatg gcaacagaca tcgcagaagt cattggtgca gcaatcgccc tctacttact
    49441 ttttgatatt ccattggtga ttgccgtctt tattactgta ttagatgttt tcgttctctt
    49501 actactgaca aaagtcgggt ttcgtaagat cgaagcactg gttgtctgct taattttcgt
    49561 cattttattc gtttttgtct atcaagttgg cttgtctaac ccagattggc acagtttatt
    49621 tgctggcttt atccctaaca gtcatacatt ctcagactca ccggtcatcg gcggcgaaac
    49681 tccgctgact ggagctttag ggattattgg tgcaacagtc atgccgcata acttatatct
    49741 tcattcagca gtttcacaaa ctcggaagat cgatcaccaa aacgaagaag atgtagcaca
    49801 agctgtacgt ttttctactt gggattctaa tatccagttg tcaatggctt ttctagtcaa
    49861 tgcactgcta ttgatcatgg gtgtcgctgt cttcaaaacc ggttctgtcc aagatccttc
    49921 cttttttggt ctgtatgaag ctttatctga tccatcagct atgagcaatg gtattttgat
    49981 tgctgtagca aaatctggta tcttgtccac tttatttgct gtagcacttc ttgcttctgg
    50041 ccagaactct actattactg gtacgttgac cggtcaagtc atcatggaag gattcatcca
    50101 tatgcgtatc ccgatttggc taagaagact ggtcacacga ttgctttccg tgattcctgt
    50161 attgatttgt gttttgttta caagtacaaa aggaacgatc gaagaacatg ccgctttgaa
    50221 taacttaatg aatgaatccc aggtgttctt ggcttttgcg ttgccatttt cgatgattcc
    50281 tttactgatg atgaccaaca gccaagcgga gatgggtagc cgatttaaaa attcattcat
    50341 cgtaaaactt cttggctggt tctctgtgat tagtctaact tacttgaact tacgaggatt
    50401 accaggacag atcgaaagtt tcttcggcga gcaagccact tccgctcaac tagcattggc
    50461 tgatcacatc gcttatgtaa tcattgttct tgtccttctc ctgttggcat ggacgattgg
    50521 tgatttatat aaaggaaaca aaaaatatga acagcttttg caggaaacgg tagaagaaat
    50581 cgaataaaaa ctgatttttc cacacaaaga cccttgatag ttaggttaaa aatctagcta
    50641 ccaagggtct ttttatttag aagataaagc ttttttcaga tttaatttac tgaaaactag
    50701 catgcttgtt actacgatga attgcgttaa tggcacgcct gcaccagtgt agacacttaa
    50761 aaaatagtcc tttttttctg gcgcataaga acaaaaccat gagacaccga aaagaaaaac
    50821 caattgtgtc agtattaatc cggtgaccac aatcaaagag gaactataat aggcacgatc
    50881 agcttttgca aatacggttc ctagtatcat aagtaatatc cctgctattg aagcaatcag
    50941 cacgggaata gccagaactt ggtaaatttt tatgatgaca ttcgcgactt ttgctgattt
    51001 tgttaattgg aaattaggtg ttctcggttc attactatcg atccaattct ccattagcgg
    51061 aaggtttaat aattcttgag cctgtttgac ttgttcttgc gttccgaaac tataatttcc
    51121 tccttgatgg aaatctttat atcgccatgc ataagcgtat cctgattgaa ggaaatcgaa
    51181 tacgattggc agatcactca tcgtctttcc atctcctgat cccatcagat agaactcttt
    51241 tttctttttg atggttccct tgtgataggc ttcttctaat tcttggttga cttttttcca
    51301 aaaatcattg gtttcttttg cattgttttg gtaataacca gattcgctta ccacatctcg
    51361 caatgcccaa aagatgatat ctccgggtaa ttcttttact tttccacctt gagtccacgc
    51421 gtgagtcgta tataattcgt caatacgggt agaaacagta tggaatgtag gagatgcggc
    51481 taatgcttta tttagtgcag catgtgagac ccatatctca ctgttttctt tattcaagtt
    51541 cgtttcatca tctattcgga tcagttgttt catcaaacgt ccaaattctc cactggaacg
    51601 gtcattgatg gttgcaatac cgtatgttgt ctcattttta tattcgatcc actgatagac
    51661 gatggaaaag ccaactatag gaagtaaaaa caatgaaagt ttcagccatt cccgtttcga
    51721 gagaagaaag agtcgttttt ttaacttctt tacttctttg aaagaaaagt cttgccctag
    51781 caagatctgg acgaaactga tacccaaagc tactacagta aaaggaagaa gccagataga
    51841 atcttcgcga atgaaccaaa agaaaggaaa agcgacagaa agccctaaaa accatggcag
    51901 caattttctg ctagaaccgt atttgcggta ataaatagct aataaacaag cgataatgat
    51961 ctcaacagcc ggaatcacga gggcattacg atatgttcga gtggaatatt catgtgtaaa
    52021 actaatcgga gaaaacaaaa aataaaaata gatacttaaa agaagatact tgttcttcac
    52081 aatcggttta aacgcgagtg caaccaatat actggcaaaa atactgaaca tgccgaacaa
    52141 aactgcataa gaaagatgca gtttatgtgc aagtactaaa aataagctgt aggagattcc
    52201 tttggaaagt gttgtagtcg tatactcccc tagccattta ccatccaaaa tattttgagc
    52261 atagtgaatc agcagctgat catcataggt cgccgacata tctgttccat agattgcttt
    52321 actatataaa tacaaccgaa taattgtaaa taatacggct atgccaagaa taaagtatgt
    52381 atgtttcttt tttagttggt cgatcaaggg gtttaaaatg ttcagcatct tttcgtcctt
    52441 tcaatcacgt tttgctactt gtttgtgaaa cttttcgttt tttacttttc tatcagtata
    52501 ccctgtttgt ctgaatttaa aaagaggggg caaaaggagc atggcgaaaa cgaatgcaaa
    52561 aagcactcct ttgtttctta aacaaaaaag gacaaggcag actgcccaat ccttttttgt
    52621 tgctgtcagg tagaaaactt aaagtgtgac ttcttctgct aataactcag ggaacacttc
    52681 gggatcgaat gcttgcgctt tgaacatgga aatttctttt ccatatggtg cgtaagcttt
    52741 ctttttatct ttaccgacat atggggtttc taatattttc gaaatgttgt caaacattgg
    52801 atcatggacg attttgttca atgtgtcaaa tccaattgtt ccaaaaccga tattggcatg
    52861 ccgatcttta tgagaacctt gtggattttt agaatcattg atatgcagta ctttaagccg
    52921 atctaatccg atgaccttat caaactcttc cattacacct gaaaaatctt cttttatatt
    52981 atatccggca tcattcgtat gacatgtatc aaaagtaacg gataattttt cattcaattt
    53041 tactccatcg atgatttgcg ctaattcctc aaatgttcga ccgatttccg tacctttccc
    53101 tgccatcgtc tccaaagcga tctgtgccgt ctgttctttt gtcaaaactt cattcaaccc
    53161 ctcaataatt ctagcaatcc cagcttctgc ccctgctcct acatgagcgc ctgggtgaag
    53221 ggtgatttga gtagcaccta aagcttccgc acggacaatc tcttctctca gaaattgtac
    53281 agcaaatgga aacatttctg gtttgatcgt atttcccaaa ttaatgatat aaggcgcatg
    53341 aacgacgata tttgttagtc cgttttctgc cataaatgct tttccggcat caatattcat
    53401 ctcttcgata gcttttcttc tagtattttg aggagcgcct gtatagatca taaaggtcga
    53461 agatccgtaa ctggctgctt cttctgccgc gcctaacagc attttctttc cgctcatgct
    53521 gacatgtgag cctaataaca tggatggatt cctccttgaa aggcaattgt ttcgaacaat
    53581 tcctgtatat tgataagatg atgtacaaag aaaatggttt gtttttcttt gccctgttct
    53641 ccatagtaac tgaaactttc cctaaagaaa agcttaagaa ctctttcata aaaaagatca
    53701 tttcctcacg ttttatctca ttcgaaaaag aaattcctgt caaaaacagc cttgtctgtt
    53761 tttttgtcct attatttgct ataattagcg aaataaaacc attagttgag gtgacccttt
    53821 tgccacgcaa aaatactcga aaaaaacaga atagaagaaa aaaagaaaaa tggtttgttc
    53881 ctaagattat ttttagagtg tttcaatcat tgactgtttt tattactgtt cttcttttgt
    53941 tgttcgcagc tctaggtatc ggaataggtg ctggctactt tgcctatctt gtcgaagaca
    54001 cacaattgcc gactaaaaaa gaactgcaga cagaactagg aaacattacg gaaacgtcaa
    54061 agattgttta tgcagataat acagagatat caaaaatcca gacagatctg atgcggacga
    54121 ctatttcatc tgataagatt tcgcctttat tgaaaaccgc gattatcagt acagaagatg
    54181 aatacttcga taaacatcaa ggttatgtcc ctaaagctgt tttacgagca ctagtttcag
    54241 aagcgactgg catcggctcg tctggaggat ccactttgac acagcaatta gtcaaacaac
    54301 agatcctaac cgatgaaaca acattcaaac gtaaagcaaa cgagatcgtt ctttctgcac
    54361 aagtagaaaa acacttttca aaaaatgaga tcattactac gtatttgaat gtctcaccat
    54421 ttggtcggaa taataagggg caaaatattg ctggagtcca ggaagctgct agaggaatct
    54481 ttggtgtaga tgcaaaggat gtgactctgc cacaagctgc ttatatcgct ggtttgcctc
    54541 aaagccctat cacgtatagt ccatacacga atacaggcgc gctaaaagaa gatctttctg
    54601 ccggtcttgc cagaaaagat tttgttttat tcagtatgta ccgtgaagga caaattacga
    54661 aagaacaata cgaagaagcg aaagcctatg atcttacaaa agactttctc ccacaacaaa
    54721 tcgcggaaca gactgacaga gaattcttgt attataccgt gatgaacgaa gcaacaagga
    54781 ttattgcaca acaattggca gaaaaagata atgctgatat gtcagattca tcggtcagtg
    54841 aagcttatta tcaaaaagca cagcaaacga tccaaaacaa aggatatacg atccattcga
    54901 cgatcgataa agatatctat gctgcattac aaaccggcgt agaaaattat ggctacatgt
    54961 tagatgatgg tgctggtagc caagtggaga ctggaaatgt attgatggat aacagaaccg
    55021 gacgtatcta tggcttcgtg ggtggtcgga attacagcca gaatcaaaat aaccacgcct
    55081 ttgatacaga acgtcaagct ggttcatcca tcaaaccggt tcttgtttac ggtcctgcaa
    55141 tcgatatggg acttgtcgga tcagaatccc gtgtatcaga ttatgcaacg acttggcaag
    55201 aaggaaaaaa tgctggtgag aaaatcgtca atgcgaccaa tgaagggtcg aacaccttcc
    55261 aaaccgtccg tgagtcactg gaatggtcca acaatatccc agcttatcac ttatatcaag
    55321 atgtgttgaa ttccggtgga tcgaaacaat acgcttacga gcactacttg gcgaagatga
    55381 actacccagc aaatgataat tggggcgttg aatcggctcc tttagggaca gtagatgtaa
    55441 cgacgcttca acagacaaat ggattccagg ccttagcaaa tggtggagtc tacgaagaag
    55501 gctatatcat tgattccatc acagataatg caggtaatgt gatttataaa catgaaagta
    55561 attctgtccg tatttattcc gaagcaacgg catcgatcat gaatgacatg atgcgtagtg
    55621 tgatcaatga aaaaatcact acaccattca aagatgtcat ctcaagctta aacggaaatc
    55681 taggaaatgc agattgggtc ggaaaaactg gttctaccaa tgaataccga gattcatggc
    55741 tgatcgtctc aactccatct atcactatca gtagttgggc aggacacgat gacaatactg
    55801 gaatggacag ccaagcaaga atacgttcgg ctaattatct tgccaatcta atcaatcaag
    55861 cttaccaagc aaaccctgag attttcggca ctgatgagaa atttgactta tcatccgatg
    55921 tgatgaagaa aaaagtatct gggttcacag gacaaacagc tggaaaagtt actgtcaata
    55981 aaaaatccat ccaaacgcca ggaaaaacag tggagtcact ttgggcaaaa aatggtccga
    56041 agaaaagcgc ctttaaattc ggcgtaggcg gtactgatga aaactataca gattactgga
    56101 acaccgcttc agcttatgct agagcgaatc cagagaccaa agaatcagac gataaagatg
    56161 attgatctaa tagattaatc agaattaaag ggagctgcga caaaaatctt gtcacagctc
    56221 cctttttccg aataaatggt gttcaaaagc ttacatctac gatattaaga caaatttatg
    56281 taaaaatgtg aaaaac
//
LOCUS       DACCDF010000008        54447 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCDF010000008 DACCDF010000000
VERSION     DACCDF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.008"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..590
                     /locus_tag="IVK51_000458"
     CDS             <1..590
                     /locus_tag="IVK51_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430079.1"
                     /translation="FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYF
                     GNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLI
                     VGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMA
                     VKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(830..913)
                     /locus_tag="IVK51_000459"
     tRNA            complement(830..913)
                     /locus_tag="IVK51_000459"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Leu,seq:gag)
     gene            1296..2513
                     /locus_tag="IVK51_000460"
     CDS             1296..2513
                     /locus_tag="IVK51_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6430080.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2697..3533
                     /locus_tag="IVK51_000461"
     CDS             2697..3533
                     /locus_tag="IVK51_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430081.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3609..4757
                     /gene="nagA"
                     /locus_tag="IVK51_000462"
     CDS             3609..4757
                     /gene="nagA"
                     /locus_tag="IVK51_000462"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP6430082.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4978..6036
                     /locus_tag="IVK51_000463"
     CDS             4978..6036
                     /locus_tag="IVK51_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP6430083.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            6055..7074
                     /locus_tag="IVK51_000464"
     CDS             6055..7074
                     /locus_tag="IVK51_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP6430084.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7176..7892)
                     /locus_tag="IVK51_000465"
     CDS             complement(7176..7892)
                     /locus_tag="IVK51_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6430085.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(7861..8646)
                     /locus_tag="IVK51_000466"
     CDS             complement(7861..8646)
                     /locus_tag="IVK51_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6430086.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8637..9386)
                     /locus_tag="IVK51_000467"
     CDS             complement(8637..9386)
                     /locus_tag="IVK51_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP6430087.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9684..10208
                     /locus_tag="IVK51_000468"
     CDS             9684..10208
                     /locus_tag="IVK51_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430088.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10224..11723
                     /locus_tag="IVK51_000469"
     CDS             10224..11723
                     /locus_tag="IVK51_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP6430089.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11811..12413)
                     /locus_tag="IVK51_000470"
     CDS             complement(11811..12413)
                     /locus_tag="IVK51_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6430090.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12517..13473
                     /locus_tag="IVK51_000471"
     CDS             12517..13473
                     /locus_tag="IVK51_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP6430091.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13778..14734)
                     /locus_tag="IVK51_000472"
     CDS             complement(13778..14734)
                     /locus_tag="IVK51_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6430092.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14908..15468
                     /locus_tag="IVK51_000473"
     CDS             14908..15468
                     /locus_tag="IVK51_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430093.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15461..16285
                     /locus_tag="IVK51_000474"
     CDS             15461..16285
                     /locus_tag="IVK51_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP6430094.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16295..16912
                     /locus_tag="IVK51_000475"
     CDS             16295..16912
                     /locus_tag="IVK51_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP6430095.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16930..17613
                     /locus_tag="IVK51_000476"
     CDS             16930..17613
                     /locus_tag="IVK51_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6430096.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18296..19579
                     /locus_tag="IVK51_000477"
     CDS             18296..19579
                     /locus_tag="IVK51_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6430097.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            19595..20482
                     /locus_tag="IVK51_000478"
     CDS             19595..20482
                     /locus_tag="IVK51_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430098.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20499..21335
                     /locus_tag="IVK51_000479"
     CDS             20499..21335
                     /locus_tag="IVK51_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6430099.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21338..21961
                     /locus_tag="IVK51_000480"
     CDS             21338..21961
                     /locus_tag="IVK51_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430100.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21958..23250
                     /locus_tag="IVK51_000481"
     CDS             21958..23250
                     /locus_tag="IVK51_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6430101.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23609..23706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23822..24403
                     /locus_tag="IVK51_000482"
     CDS             23822..24403
                     /locus_tag="IVK51_000482"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP6430102.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24406..25713
                     /locus_tag="IVK51_000483"
     CDS             24406..25713
                     /locus_tag="IVK51_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP6430103.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            26128..26616
                     /gene="purE"
                     /locus_tag="IVK51_000484"
     CDS             26128..26616
                     /gene="purE"
                     /locus_tag="IVK51_000484"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP6430104.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26600..27733
                     /gene="purK"
                     /locus_tag="IVK51_000485"
     CDS             26600..27733
                     /gene="purK"
                     /locus_tag="IVK51_000485"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP6430105.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSIEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27746..29041
                     /gene="purB"
                     /locus_tag="IVK51_000486"
     CDS             27746..29041
                     /gene="purB"
                     /locus_tag="IVK51_000486"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP6430106.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(29221..29598)
                     /locus_tag="IVK51_000487"
     CDS             complement(29221..29598)
                     /locus_tag="IVK51_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430107.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29854..30582
                     /locus_tag="IVK51_000488"
     CDS             29854..30582
                     /locus_tag="IVK51_000488"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP6430108.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30582..30836
                     /gene="purS"
                     /locus_tag="IVK51_000489"
     CDS             30582..30836
                     /gene="purS"
                     /locus_tag="IVK51_000489"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP6430109.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30838..31509
                     /gene="purQ"
                     /locus_tag="IVK51_000490"
     CDS             30838..31509
                     /gene="purQ"
                     /locus_tag="IVK51_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP6430110.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31510..33732
                     /gene="purL"
                     /locus_tag="IVK51_000491"
     CDS             31510..33732
                     /gene="purL"
                     /locus_tag="IVK51_000491"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP6430111.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33717..35156
                     /locus_tag="IVK51_000492"
     CDS             33717..35156
                     /locus_tag="IVK51_000492"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP6430112.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            35188..36231
                     /locus_tag="IVK51_000493"
     CDS             35188..36231
                     /locus_tag="IVK51_000493"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP6430113.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            36228..36806
                     /gene="purN"
                     /locus_tag="IVK51_000494"
     CDS             36228..36806
                     /gene="purN"
                     /locus_tag="IVK51_000494"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP6430114.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36803..38338
                     /gene="purH"
                     /locus_tag="IVK51_000495"
     CDS             36803..38338
                     /gene="purH"
                     /locus_tag="IVK51_000495"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP6430115.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38354..39628
                     /gene="purD"
                     /locus_tag="IVK51_000496"
     CDS             38354..39628
                     /gene="purD"
                     /locus_tag="IVK51_000496"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP6430116.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39750..40664
                     /locus_tag="IVK51_000497"
     CDS             39750..40664
                     /locus_tag="IVK51_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP6430117.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40797..42362)
                     /locus_tag="IVK51_000498"
     CDS             complement(40797..42362)
                     /locus_tag="IVK51_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP6430118.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42355..43806)
                     /locus_tag="IVK51_000499"
     CDS             complement(42355..43806)
                     /locus_tag="IVK51_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6430119.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTIEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43820..44836)
                     /locus_tag="IVK51_000500"
     CDS             complement(43820..44836)
                     /locus_tag="IVK51_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430120.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45056..46144
                     /locus_tag="IVK51_000501"
     CDS             45056..46144
                     /locus_tag="IVK51_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6430121.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            46198..47724
                     /locus_tag="IVK51_000502"
     CDS             46198..47724
                     /locus_tag="IVK51_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP6430122.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47726..48931
                     /locus_tag="IVK51_000503"
     CDS             47726..48931
                     /locus_tag="IVK51_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430123.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            49188..50165
                     /gene="guaC"
                     /locus_tag="IVK51_000504"
     CDS             49188..50165
                     /gene="guaC"
                     /locus_tag="IVK51_000504"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP6430124.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50429..51853
                     /locus_tag="IVK51_000505"
     CDS             50429..51853
                     /locus_tag="IVK51_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP6430125.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52314..53423
                     /gene="pdhA"
                     /locus_tag="IVK51_000506"
     CDS             52314..53423
                     /gene="pdhA"
                     /locus_tag="IVK51_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP6430126.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53426..54403
                     /locus_tag="IVK51_000507"
     CDS             53426..54403
                     /locus_tag="IVK51_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP6430127.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17402 a         9316 c        11922 g        15807 t
ORIGIN      
        1 tttttttaga catgaatatc ttatttttat tgctattttt cttaattttc cttctaacat
       61 ataggaaatg tcaaaactat tatgtgcgta tattgactgc attaccgttt ttcttaaacc
      121 tgattattta ttttggtaat acaatgggtc aaagctttac ttacgtcaat ggaaataaaa
      181 gggcaatgat ttggagtagt agtaacctga ataatctctt tacagagtta ggaacgaaac
      241 tgtcactttt ttatccaggt acttggatag ctactttagt agtcttggca ttattactat
      301 gtttaattgt tggaatttat ttaagttttg acaataagaa aacttctatt tttttagtta
      361 ttttgatgat aatgggattt tgttcaaggt taattatggg attttctccc acagtatggg
      421 cttccggaat gcggacgtac tatatacttt atgttgttat cgccatctta gtactaatgg
      481 cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa tgaatttatg caatttggat
      541 taaccgtgct tggtatttgt acctttataa taacggtgat aaacagataa tatacaaaaa
      601 atccctcgct ttagtattag gtgagggatt ttactcgttt ttcgattaat tgttttaaca
      661 cttgcaagtt tctacgagta gcttgtttcc gaccttcata tactcacctc aggctgttta
      721 catgattgta cgttcttttt gtaaaggata cagtgatatg tatataatgg cataaagaaa
      781 cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac gagttgtagt gcagatgatg
      841 ggactcgaac ccacacgagc taacgctcac atgaccctca atcatgcttg tctgccaatt
      901 ccaacacatc tgcgtaataa taacacacta atgatagcga ggttttacat agttgtcaag
      961 ctcaaaaatg ggaggactta ttaagatgga gaaatatgac ggagaatttt caggactggg
     1021 aatgatctta ggtgtattga ttggacttgc attcggacgt tttttgcttg gttttatgct
     1081 aggaatcatt tgtggaattg caatggattg ggcagcgaat ctatggaatg actatcatga
     1141 caattaagtt ctcaacgctt gtttgtgttt ttttaatact tacgtttttc ctaaataagt
     1201 ggcaaaattt tctaggatgg agtatgatat tgaaatagta aataacaaaa aatttggaaa
     1261 gttctaactt tttgaaatga gggattggct aagtgatgag agaatttgaa aaatccaaca
     1321 aattagaagg tgtcagttac gatgttcgcg gtcccgttct agaagaagcg gagcggatgc
     1381 aagaagaggg cattagtatt ttaaaattga acacgggaaa tcccgcacct tttggatttg
     1441 aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc ccgtgactca gaaggctact
     1501 cagattccaa ggggatcttt tctgctagaa aagcaattga gcaatattgc caactgaaac
     1561 attttccgaa tgtgacgatc aatgatatct acaccggcaa cggagtgagc gaattgatca
     1621 cgatgtgtat gcaggggttg ctggacaatg gagatgaagt actggttcca atgccggact
     1681 atccgttgtg gacggcttct attgcattag ctggaggcac acctgttcat tatatttgcg
     1741 atgaagaggc agaatggtat cctgatattg atgatatcaa atcaaaaata acgagtcgga
     1801 caaaagctat tgttatcatt aatcctaata atcctactgg ggcactttat ccaaaagaat
     1861 tattagaaga aatcgttgaa gtagctcgac aaaataacct gattatttat tccgacgaaa
     1921 tctatgatcg cttagtgatg gatggtctag aacatattcc gattgctaca ttagcgcctg
     1981 atttgttcgt tgttacgctg aacggtctat ccaaatctca tcgtgtagca ggatttcgag
     2041 taggctggat ggtcctaagt ggagataagt cacacgtcaa aggctatata gaagggctga
     2101 atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc tcagcaaatt atccaaaccg
     2161 cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc aggcggaagg atctatgaac
     2221 aaagagaata tatctataat gcaatcaatg atattcctgg tctttcagcc gtaaaaccaa
     2281 aagctgcttt ttatatattc ccaaaaattg atacaaagcg attcaatatt ttggatgatg
     2341 aaaaattcgt tttggacttt ttacatgaac atcatattct acttgtacat ggtggtggat
     2401 ttaattggaa ccagccagat cattttcgaa tcgtttatct tcctaaaatg gatgatttga
     2461 aacaaacagc taagaaaatg agagaattcc tagcaaccta tcgccaaaaa tgattttaaa
     2521 aaccaaattt ttcagtagga gggccacaga caaacacact gaggctctct ttctagtgga
     2581 gttttcttaa ataactaaag tatattattt agttgcaagt cttttcttga agtttgcgac
     2641 ttcctaaagt aaaatacgaa tagaatgcga atacgcatat tttggggagg atacttatgg
     2701 aaacgttaat cgagtccttt ggaacgatca ttaaagagat tcgaaaagaa caaaaaatga
     2761 cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt attaagtcgg atcgaaaata
     2821 atgaggaact gccgaatgta ttggtgatga tgcagatttg tcaacggcta ggggtaacga
     2881 ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac aaacgataaa ttatttgaac
     2941 tgttggatag ttgtttccaa aaacgtgatt atcggaagtt agaacaagta ctaaaatctt
     3001 ctgatatttc tgaaaatctg tatcaagcaa ctgactttca acgctattat tattatctcg
     3061 gtgtttgcga attcacgttg cgtcaaaata tctctcagtc cctttattat ttgaaagaag
     3121 cattaagcta ctctagccaa aaagaaagaa tccatgtttc tgacacagaa atacaactga
     3181 ttagttgtat cggaaaaata tatggtgtgg caggaaaaac ggctgaagca cgctactatt
     3241 tgagtcgaag catccaactg ctgcatcaat cagctgatga acgatcaaaa agtgaatttt
     3301 cgcaaatttt ttataattat ggaaatttct tattccatca aaatgaaatc gacgaagcgt
     3361 tagaacaggt gaatcaagga atctattggg ctcaagagaa gaatagctac tattacctca
     3421 atgatttatt tgtgcttaaa agcttaatat acaagcgtaa agaagaacca gaaagagcag
     3481 cattctatga aggcttagca caagctgtga aacaaatttc aaaaaatttg taaatggtgt
     3541 agaccattct tgacttcttc tctaaataag agtactttag aggtagaaaa acaaagggag
     3601 agaatagaat gagaactttt atctatgctg acaaattctt tttagcgtca ggtgtcaaac
     3661 atgcaggcta tcttgaaatt accgatggac tttttggtga atacaccaaa gaactacctg
     3721 agggagaagt caacatcatt gaccaaagcg gcaaatggat tgctcctggt ttggtagata
     3781 cacatattca cggctatatg aatcatgatg taatggataa tgatgcagaa ggcatcaaag
     3841 tgatgtctga aggtctgtta tcttgtggag tgacttcatt tttaccaaca acattgacgt
     3901 caagcaaaga acgtttaaaa gatgtagctg agacaatcgg caaaatgtat caggaagcac
     3961 caggagctaa aatcaaagga atctattttg aaggtccttt cttcacagaa gaacacaaag
     4021 gggcacaaaa cccaagttat ttcggggatc ctgatttgga tactttccat gaatggcaag
     4081 aagcttcagg tggtttaatc aaaaaaattg ctttagcacc ggaacgtcaa ggtgtagaag
     4141 agtttgtcaa acaagtgact gaagaaggag tagttgtttc tctaggacac agtaatgcta
     4201 cactagaaga agcgcaaaaa gctgttgaag caggcgcaag tgtatttgtc catgctttca
     4261 atggtatgcg tggattgaat catcgcgaac caggtatggt gggtgcgtta ttatctttag
     4321 aacatgtctt ttctgaattg atttgtgatg gccatcatgt acatccaaat gctgcgggaa
     4381 tcctaatgga aaaagcagga catgatcatg tcgcattaat tacagactgt atgatggccg
     4441 gtgggatgcc tgatggaaat tacaatttag gcgaatttcc agtagtagta aaagaaggaa
     4501 cagcacgtct agaatctggc aacttagctg gaagtatatt gaaattgaaa gaagcaatta
     4561 aaaatgttgt agactgggga ttagcaacac cagaacaagc agtcatgatg gctagtctga
     4621 ttccagcaat cagctgtaaa atcgatgata catgtggaaa aatcgctcaa ggacgcgatg
     4681 cagactttat cgtattgaac cctgatatga cattggaagc aacttatcta gatggcgtgg
     4741 aacgtttcca cgcataatat aatttaagtt aaccttaaaa ctaaacttct taagtttggt
     4801 tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca taagtttcaa aagtttcttt
     4861 aatttcctat tgttcaataa gtaacaatgt aaagataaaa ttaaaaatgt taatacgtat
     4921 tagcaaaata aaagcttttt ttattgtgaa taaatgaaca taattaggag gagaaagatg
     4981 aacttatttg acatttttcg atcgactttg acagatatgt cgattgtcag tgcaatcact
     5041 tcaacagttt ttattatatt attagggttt gtttgtagaa aaaaggggat tttttctgaa
     5101 tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg ctttgccagc actggcattt
     5161 cgagctttca tgcaagatat caatacagaa ttactgatgc aaggaatcaa tgtcttgatt
     5221 tggggattag ttatttatat tattcttatt ctagtttcaa aacctttttt cttaagctat
     5281 aaaggggata agcaaacaac gttgcgtgtc ctgacgatct ttggttctac gacctttttc
     5341 ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg tgatgtattc ctcaattttc
     5401 aatattggct accgaatctt tttgtattct tatggataca taaaaatgag tggcttgaag
     5461 atggaagcta aaaatatcaa agcgatgttc gtcaatccaa tcattatcgc tacttttgcc
     5521 ggccttttca tttggctgtt ccaagggtat ctgccacaag tgtcagttac aggtgcagaa
     5581 ggagagataa gccaagtagc gtttttgcga attgaccaga ctgcaccgtg gctatttcag
     5641 ccgatggatt atttagctgg tttaagttca ccattggcgt ggctagcaat cggcgcaaca
     5701 ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa cttcatggta ttacagtcta
     5761 gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc tagcagtttt gacggcaacg
     5821 aatattctac cagtcaattt tgaagcactg gcaacaatcg ttatcatgat ggctacacca
     5881 gctgcaacag tggcagcagc ctatgctatc agttttgata gggaggcact tttagcctcc
     5941 aatgcatcct tactgtcaac tatttttgct gtggtactga cacctgtatg gatcgttgta
     6001 ttacaaatga ttcatggcat aggtttattt tcataagaaa tggaggaaga aagtatgaga
     6061 atcgcatgtt acggtgttcg tccgaacgag atagattttt ttgaaaaatt gaatatttac
     6121 cattatgatc tcagtcttta tgaagaactg ttgacacatg acaatatcgt gtcagcaaag
     6181 gaccatgatg ctgtattgtt gaggggaaat tgtatagcag atgagaccaa tcttgcaaaa
     6241 atgcaggaat atgggattcg ttatgttttt acacggactg tgggagtcaa tcatatcgat
     6301 ctacaagcgg cagctgattt tggtatgaca gtagcccgtg taccatcgta ttcgccaaat
     6361 gcgatagcag agttatcttt gacttttgcc atgatgcttt tgagaaatac tgcgtatacg
     6421 acgatccgaa catctttcaa agattttcga gtggatgagc aaatgtttag tcgtgaaatc
     6481 agaaattgca cagtagggat cataggaaca ggcaggatag ggttgacgga agctgttttg
     6541 tttaagggac taggagccaa agtgatcggc tacgatattc atcagacaga agctgctaaa
     6601 aaagtattga cgttccactc gcttgaagag ttattagcca aaagcgatat tgtcagtatt
     6661 catgtacctc atatcccagg tgaaaacgat caaatgatca atgaaggatt tttagctcaa
     6721 atgaaaaaag gtagcatctt gattaataca gcccgtggag agcttcaaga taatgaagct
     6781 attttaagag ctttgaaaag caatcattta gctggttttg caaccgatgt ttttgcaaat
     6841 gaaacagagg tttttttccg atccttcaag ccatgggaga cgattccaga ccctgctatc
     6901 cagcaattag tagaaatgta ccctagagtt cttgtaaccc cgcatgtagg ttcaaataca
     6961 gatgaggcat tatcaaatat gatttcgaca agtttcgaga actttcgtga aataatagaa
     7021 actggaaaaa cgaaaaatga ggtatctctt cctaaagccc gtcaattaag ataagaaaac
     7081 atggatattc gtcaataaaa tgtttaaaaa tagtccaagt aaacagttgt tacttctgta
     7141 tacttggact attttgtatg ggataattcg atgactcaac agtaatctgt gagctttttc
     7201 cgcccattct ttgtcaattc ataacaggtt agcggccggc cgatttccaa atatttgatt
     7261 ctttcgttca atatatcttg ttctactaaa aaattcaaat atttcttgac ggatatacgt
     7321 gacagtgagc ttttttgagc cagttctttt gttgaaaaaa tagtatcttc ttttagaata
     7381 aatgcactta ttttttttaa agtcagtttt gacagacctt taggcaaatc gatgaatgat
     7441 ggtatttcgt gaatgggagt ggtgtggttg aatacttgat cgatcaattt ctgatcggct
     7501 ttttcagtcg tttggattgt ctgagagaaa gcaagatact tttcgatcgc cagctgaaaa
     7561 cgttgcaaag tgaacggttt gatcaaataa tctaaggcac cgtagcggat cacattcttt
     7621 atggtttcga tatcatctgc agcagtgatc aaaatcactg ggatatcatt tccttgctgt
     7681 ctaagccaag ataaaaaatg gataccactt tcgccaggca tgtatacatc aagcagcagc
     7741 agatctgcgt ttttttgtct gaaatatttt tttccttctt ctacatttct gaaactttcg
     7801 attacgttca tattagggat gctttccaca aaagtacgat tcaattctgc aaccattgga
     7861 tcatcttcga ttatcaggac atttgtcatc attttgatct tccttttttg gtaattctag
     7921 atagacgatt gtaccgccat ttgtttgttt ggaaaattct aaaaatccac cgtggcgttt
     7981 gacgattgaa tggatgagat tcaatccaaa cccacgattg attcccttcg tcgaaaaacc
     8041 tagcgtaaat attttttctg agatttcttc tgatatcccg acaccgtcat cgcagacggt
     8101 cacgatgaat acttcagctt ctgtgtcata gtttaaatga atccggacct gtccgtttct
     8161 ttcatgtttt tgtagcacgg cttcttttgc attatcaagt aagttcccta acacttgcaa
     8221 aagtgaatgg atggagtcat ccatctccat tttagggata aaagaatcgc tttctaacag
     8281 catttcaaca gattgttctt tggcttcgtt tattttgcct aataaaaaac cggcaattgc
     8341 aggtactttg atcagacttg agacttggcc aatctctttt ttataatttt gatttagctg
     8401 ctggataaaa tgggtgactt cttcatactt cttcaaatca atcaaaccag aaatgacatg
     8461 aagtttattc atgaattcat gggtttgagc tctcatggag tttatatatt gtttcgtccc
     8521 cccaagctcc tccattaatt tcattacttc tgatcgattt cgtaaagttg cgactgctcc
     8581 taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa atagtttcta tcccatttaa
     8641 atacatggat tgagtccgaa cggtcgtttg atgctcaatc gtctctttaa acaaaacgtc
     8701 atatagatta tcactgattt ttttcctact tcgaaatcca atagctgctc tgtttggtag
     8761 aaaatgtttt ttgcaggtgt attgatcaag aggatagttt tatcagaaga gatggctaag
     8821 attccttcat ctacttcatt ttcgataatc gttttttcac ttaactgttc tgctatttct
     8881 tgtggttcaa gtccaagcag tacttttttc attttttggg caagaaaaac ggcgcctatg
     8941 atgcctacgc taagtccaat aatcaatgaa aaaacaatca ttttttgtcc atctttgact
     9001 tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg caccaataat ctcttgatca
     9061 taataaatag ggacgaaata gcggaagccg aaccctaaca ttccttcttt ttcagagaaa
     9121 aattcttgtc ctccaatcac ttttttcaca tcttcatgac ttgagaaagg aagaccgatg
     9181 taacttgggt ctgcgtgaga atatcgaatg ccttcagggt ccatgacaac gataaaatct
     9241 aaattcgatg caatcatgat ttcatttgtt attccttgaa tcgcatgtgt cgggtcacca
     9301 gtctgcagct ttttttggat gagtttatca ttggcaacga ttttagcaat gctagagatt
     9361 ttatctgaca cactagatcg gttcatcgaa agaataaaat tcctgatcaa tatggcagaa
     9421 acaatcaaag aaacgaatgt ggagaaacaa acgacaagga tgatcgtgtt ctggagcttt
     9481 aatggttttt tcttaaaaaa aggaaacata ggctacctcg aaagaattta ttgtgaaaat
     9541 tttaacatga aaacaaaaat ttaacaaatt gtcaacgata aacacaacct tcctataaaa
     9601 aacaccactg agtcggtaaa agaagctata cagaattttc aaacattggt atgatagacg
     9661 tagaaatgga aggaggcgtg tatatgatag ggggaatcgc acttgagacg atttattggt
     9721 atacattgct gatttgtgca ggattggcag tcctattgat tatttttgga gatgtattcg
     9781 attttgatgg cccgcttgat ccgatgctta ttatcccttg gttaactttc acaagcttat
     9841 ttggttttct aggtgagcgt ttaacagatc aatcatcttt atttttattt attgctagtg
     9901 gaggcatatc gatagtgttg gtatttttgc ttaatttcta tatattgatg ccgatgaaac
     9961 atgccgaatc aacgatgtcg tcttctgaaa aaactctgga aggacaagta gctgttgtga
    10021 tcacaccgat ccctgtacga ggtatgggag aaattcagtt gaaaagtgtt accggttcca
    10081 ttagtcgtcc agcttgtttt tacagccccc aagaacagac agctgagcgg ggaacgaaag
    10141 tattgatcat tgagatcaag gaaagaatct gttatgtcat gccttatgaa agcatgataa
    10201 aagcttaaag gagcgtgtgt taaatggaaa taccagttgt ttttatcgcg atcgcaatcg
    10261 cagctttgat tttactgatg ttgctgatcg tctttgtagc gaagtaccag acagcaaaac
    10321 cggatgaagc gttgatcatc agtggaagtt atttaggaag caaaaatgtg cacattgatg
    10381 aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc attcgtcttg ccagtttttc
    10441 aacgttcgaa tcggatcagt ctattatcga gtaaattgga cgtctccacc cctgaagtgt
    10501 atacggaaca aggcgtaccg gtgatgtgtg atggtacttc gatcatcaaa atcggttcat
    10561 ctgtagaaga gattgctaca gcagcagaac agtttttagg caagacaaga gaagaactgg
    10621 aaaatgaagc aagagaagta ttagaaggac acttgcgctc gattcttggt tctatgactg
    10681 tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag cgtacaagaa gtcgcttctg
    10741 tagaccttgc aaaaatgggg ttgatcatcg tttctttcac tatcaaagag gtcagagaca
    10801 aaaacggtta tcttgactct ttagggaaac caagaatcgc ccaagtcaaa agagatgcag
    10861 atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa aaaagctcaa gcagaaaaag
    10921 aatcacaagc agctgaatta cagcgacaaa cagagatcgc agaatcatta aaagaaaaag
    10981 aactgaaatt agcaacctac aaacaagaac aagatgttgc taaagcaaaa gcagaccaag
    11041 cctacaattt agaaagtgcc cgagcacaac aacaagtaat cgaacaggaa atgcagatta
    11101 aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa agaaatcaca cgtcgtgaaa
    11161 agcaatatga ttcggaagtg aagaaaaaag cagatgccga tcggtacgct aaagaacaag
    11221 aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga agcagaacga ttccgtgtgg
    11281 aagcactggc agaagcggaa gccaataaga cacgtttagc tggacaagca gaagccgaag
    11341 cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca aaagatcgcc aatgccttca
    11401 aggaatatgg cgaagcagct gtcttgagta tggttatcga tatgctaccg caattgatgc
    11461 gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc tgttgtcgat acaggaagca
    11521 gttcaggaga aaccagtgga gcgaaccgag tgacaaatta tgccacaaat ttgctttctt
    11581 ctactcaaga aacactaaaa gcgacaacag gacttgactt gaaggaaatc gtagagaatt
    11641 tgtcgacacg tgggactact agccaagtaa atatgtatga agcgccaaaa gaggcagaac
    11701 aaaactcgat gaaggaagaa taaagtagta cacttcaccg ttcaatcaaa aatggaggaa
    11761 gactttttaa agttcttctc tccatttttt tattttattg caaaagaaaa ttagtcctcg
    11821 ctatttttct tttgtttttg gatcttagct tctatctctt gtaaatttgt tcttagcaat
    11881 cgttctttct tttggagttc gaccaaatct tctgatagtt cttccttctt ttctgtttca
    11941 ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg tccctataaa agtatctagt
    12001 acacgttgga tagcatacaa agcggattcg ccttgaggga tgctcaacgc aatcagcaac
    12061 aacgtagcta ttgcggaaat gataccggaa ttattgttaa taccatctga cacgacgatc
    12121 acaataataa ctagaatagg aagcaagatc aattctacca aaaaatcatt gtgaaaatag
    12181 cttttgacaa aaaaataaat gatagctaat gtaccaccta atgtattgcc gatgatccta
    12241 gactttccaa aagaaacggt tgtagtcaag tcctgacgca aagaaaaaac agcagataat
    12301 gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa acaagataca taaaaaaaca
    12361 gctaaggcac ttttgatcgt acgcatacct agtcgaaaac ggccaaattg catagagatt
    12421 ctcctttttg gtctttttac tatcatacct aaacttttgt ctgatagcta gtttttcatt
    12481 attcaagtat aattaaaata aaaaaggggg aagtaaatgc gtataggaat gcgaacagtc
    12541 aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg gtagcttatc gctgttgtat
    12601 tggccagctg cagggatcat tgcattatta agtgtaggaa ataccaagcg ctctacattg
    12661 cgtacgggca tgaatcgaat cgcagccttt atattagcta cggcaacagc actgttcagt
    12721 ttttttgctg ttgggtatac tatttttggc ttcagtcttt ttttattcct gttcatcccg
    12781 cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa attcggtttt ggtcactcat
    12841 tatttagtgg aggaaaacat gtcttggcag ctgatcggca atgaagcggg attaatggcg
    12901 attggattag tatttgcttt gcttgcaaat gtttacatgc cagatcgtac gaagcagctg
    12961 aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata ttttgagaca aatggcagaa
    13021 tttcttttat cagaaaataa agaagatgtg cagatcaaat gcgaacattt actgacattc
    13081 attcgcgaaa gccaagaaga tgccagagaa catcaagaaa actactggct cagacagccg
    13141 ctatactacg aaacttactt ctctatgaga agagcacagg ccaatgtgat taaagacatg
    13201 ctggaaaatc tagagagaat ccagcaacca gcttattatg gaaaacatat ctatggttta
    13261 ttgatttata ctgcagaaac attttcagag tcaaatgatg gcagacaaat tttaacacgt
    13321 attgaagaag tttatgtatt gtatcggcaa atgcctctgc cgactagtcg ccctgaattc
    13381 gaggatcgag cagaattgtt ccagtttttg cagtctttta aaagttttat tgaaatcaag
    13441 gcagaattct cccaacaaaa aatccaaaaa tagaggttgt gaattcagct aatattcagg
    13501 tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt ctcctgtcaa ctcctcggtc
    13561 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
    13621 ggagctgaac ggtaggctca caacctcttt cttaaacggt gttcaaaagc tttcctctgc
    13681 ggtaataagt ccagccaaac gaaagatagg aggagctatt tttgtttggc tgttcttatt
    13741 acttgatgca gaacagcttt ttcacaacct tttaaaatta catatttttc catagaactt
    13801 gcatttgatc caagatttcg atgacatctt ttgtcaagaa gagagagcga ttgggcaagt
    13861 caccttcaat catgtgtacc atattcttta tttcgtaatt cattgcttca gatgtattcc
    13921 cgctttcgat gaattccttt gtaccatctg taaataagat ctccgcacga tcagcacgcg
    13981 ggtaatcagc aatcgtcaca taaccgttct caaaggcaac gataccgact tttggcattt
    14041 ttgcctgaaa agtcaagctg acagcagcaa gttcattttc tttgttacgc aagatagtga
    14101 cggattgttc gtccacgcct gtttcaaaag gaaccattgt agaggcaact acttcaggtt
    14161 gagagctcaa aaagaaacga gcgaaagata cagcatacgt accgatatcc aacagagctc
    14221 ctccagctaa ttctggattg aagaaccggt ttttagggtc tggttctttg taactgccga
    14281 aaggtgcttg gatcattttt aaagccccca gttttcctgt atccattatt gagcgtaact
    14341 gctgataaag tggcatgttg aagattgtca tcgcttctgc taagatcaaa ttcttttctt
    14401 ctgctaagcg catagcgtca gccagttctt ttttattcat cgtgatggct ttttcacata
    14461 gaacgtgttt gcctgcttcc aatgctgcca agatatggtc gatatgctgt ctatttggta
    14521 cagcaatgta cacgatgtcg atttcagcat cgtctaacat ttcttgatag cttccgtaag
    14581 cttttggaat attgtaacgg tgagcgaagt tttccgcttt ttctgaagtt ctggaagcaa
    14641 ctgctgttag ttcgctcgtc tcttgattga agctttcggc aaactcatgt gcgatggtac
    14701 caagaccaac gattccccat tgatattttt tcatctgaaa acgctccttc atagttggat
    14761 tgagtttctt gtctgtctat tattataaca aaaatcacga agaataaaga ggagaggact
    14821 tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg ctttttgatc aaaacttaag
    14881 tagaatagtt aaaaggaggg gataaacgtg catcaagtat atattgatct tattatgagt
    14941 gtattattag aacagtttga aactgaaaaa aattttttag atgaatactt gctgatcagt
    15001 ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc tgccatctga agcgatgcag
    15061 aaagtaaaaa atcttttcac tgattatgaa tggatgctaa cgcaaaagat tttgcgtcaa
    15121 acgattattt ttcccgaaaa acgaaacgtt gccgttgcag agtacaagca aatcaaaaca
    15181 cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag tcgagctgat ccctttgaaa
    15241 aataatcagc aggctggtag ataccttgac ttgaaagtaa gtattcaata tgatgaatgg
    15301 ggctatgatg atattttgaa ttttcgtctg ccagcagatg ttcaaaaaca gatcgaagga
    15361 gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg aagaaactta cgttcatgag
    15421 gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa gtgaataaat ttttaggatt
    15481 tggagcagtt gtgattatga tcgcagcaat gggctatgtt atttttagcg atgatgattc
    15541 gcatgttcaa gccggcaatc aagcagtcga acgaacaagc accaaaacaa ctgctagtaa
    15601 agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac aaagaaacaa aagatcgtac
    15661 gtccaaacaa ttgccagatg ttaaaacaga cgattggaag ctagttttgg ttggtccaga
    15721 tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg gcatcattaa gtaacggata
    15781 cttgatcgac aaacgcattg ctccagaata tgaagcattt gccaaggctg ctgaagcagc
    15841 tggatttcca ttagtgatgg tctcagctta ccggtctgta tcatcccaac aacaagtttt
    15901 cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta tcagaagagg aggcgacaaa
    15961 gattactaaa gaaacgatta cggagccagg atacagcgaa caccacacag gtctggcagt
    16021 cgatgtcgtc gatcaaaact ggtacaacag ttatccttct actgtattgg atgcaagtta
    16081 cggagatcag cctggagcta aatggatcgc agataatgct gcgcagtatg gatttatcgt
    16141 tcgttatcca aaaggacacg aagatataac taagatcaca tatgaacctt ggcacttacg
    16201 ttatgtcggt aaagaaaacg ctgagtatat caccaaacac caattgacgc tagaagaata
    16261 tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg aaaaaaaagt ggaaaaagca
    16321 aaagcgcaag aatatccgtt ggccagcagt ggttgccggt gtagcagtga tactcttagc
    16381 ctttgttttt tccttgaaga atctgtcttc tccttttact gataatcagc aagaaaatga
    16441 agcgcaatcc catcagcaat ttattgaacg gcttgttccg catgcccaag aactacaaga
    16501 tggttatgga gtgcttccta gtatcatttt aggacaagct atattagaat caaattgggg
    16561 aaaaagtacc ttggctagca aatacaataa tcttttcggg atcaaggctt atggggatca
    16621 gaagaaagta agcctggaaa caaaggaatt tgttaacgaa gaatggataa ctatccaagg
    16681 agacttcaaa gtgtatgatt catgggaaca gtccatggat gaccatacac aactgttcgt
    16741 ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg attacggcca ccaactatca
    16801 ggaagccgca caagcattgc aagatgcagg ttatgcgaca gaccccggtt atgcgcaaaa
    16861 aatcatccag gtcatcgaga cgtatcaatt gaatcagtat gaccggagat agcaaaggag
    16921 aagtggctga tggcagaaca atttacgcca gaaatcatga cggaattgag aaaagatatc
    16981 gtaagagtac cggaaatcat tttagaagca agcgggatca agatatttgg aaaattgatc
    17041 cgttcgatca tctttactac ggatattgcg atcatacgta atacaaatgc gaatgcagta
    17101 attgctgtgt atccttttac acctcatcca gcgattacta aaagcattat tgaggcagct
    17161 gacattccag ttttctccgg tgtaggtggt ggtttgacac aaggtgctcg ttcttcctac
    17221 atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg ttttgaacgg tcctacgcct
    17281 ttagagacag taaaagaagt ttgtcgagta gttgatattc cagttatcag tacggtaacg
    17341 tcgaaatata cagaaataga tgaaaagtta gcagctggta tttcgattat caatatcagt
    17401 gcaggtaaag aaactgcaga agcagtacgc cattttcgta aaaaatatcc tgaacttcct
    17461 atcattgcga caggtggtcc aacggaagaa agtattgctg agaccattca agcaggagca
    17521 aatgccatca catatacgcc accaagtagc ggagagcttt ttagtcgtaa aatggaaaaa
    17581 tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga gaatcaaaag acgaggaaga
    17641 gcagatagtg gctcttcctc gtcttttgta ctccagtact atttgttcag aattcaaaaa
    17701 aaagagaaat ttttttgttt ttttacctgt aatgtacata agaagagtta aaaccttttg
    17761 ttattattag cattctaaca aaaaaatgtc ttgatttatt gaagcgcttt ctatagaatg
    17821 aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag tagcagctaa attgtcgggg
    17881 cttgaaaagt cagcgggaac tgaccaatca agttgccata gtcaatgctt gtgcatcgac
    17941 gtgtatattg tacctaattt catacagaat tactagttat ttttctgttt tttagggaag
    18001 agcgcacacg aagaagcatg cttatttagc tatgtcaagg atattgggca gttgtctgat
    18061 atcctttttt ctttgcgtaa ttatcctccc atcattgaag caagaagtgt ttttagcgat
    18121 gaaagaagaa tcagcactat ctaatttttc ttgaaagtat tgagttgttc atcctaggca
    18181 gaacgataag gcaagtcttg gtttttattc cccttgaaaa agatcgttta ataaatatgt
    18241 tctgtataaa aatacagtat attgaaaaat aacaatggaa gtagaggtgc agagaatgaa
    18301 gaaaagaatg agaatcatct tagctttaag tgttatgtta ttcttcttga cggcttgtgg
    18361 gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg tggggttcgc aagtccgaca
    18421 cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa aatccccatg tgacgatcga
    18481 ttatgaattt tatgattatg aaggatatta cacaaaatta ggtacactag cagcatcaga
    18541 tgatatctgg gacattttcc aactgaacaa tcaatttcct caatacatca atcagattga
    18601 accattagat gaatatgtgg agcaagggat aattgatatt tctaatgcag atgaatccta
    18661 tttagcaacg acgacttacg acgggcaatt agttggactg tctaacgggg tgaattcgtt
    18721 tgcgattgct tacaataaaa atatatttga acaattaaat atccctgaac cagcttcaaa
    18781 ttggacatgg gatgaatttg aacaaatcag ctatcaaatc actgaagaat taggaatcta
    18841 tgctatttca agattcgaag attttatagc tggctgtatt attcaaatac cacaagttga
    18901 gaaaggattg aatttttata atcgggaaga tctatctaac agcttgggat ttgaaaatcc
    18961 agactatcta acagattttt tccagctgcg taaaaatctg gtagacaaag gggcccatcc
    19021 agaaccaggt ggtggagctg caagtacgac ggatctgtct tttcaggcgg ttagggatag
    19081 agaagcggca atggtgtttc tatcaagtaa ccagttgaca gaaatattgt caggggctcc
    19141 tgaagatatg gagattcagt tgattaatcc acctcgcaga aaagcagatg gagaatctgg
    19201 tatcccttta cgttcttcgc aaatgctgag tatggcaaaa agctcagccc ataaagaaga
    19261 agctgctaag tttattgatt tctttcaaaa cagtgaagaa gccaatgaaa ttttaaaagg
    19321 agaacgtggt gtaccaatca tgtcgaatat tcgagaacag ctcgctcgtg cagatgatgc
    19381 aactttgcaa gcgacatttg attatttaga aatgatttcc gatatggaca acggcgaagt
    19441 gaatgtcttt gatagtgctt tgaatccgga aattcaagac caatacaatc ttttagttga
    19501 aaaagtcatt tatgaagaag taacagcaga agaagcagca gagagtcttt atgagtttgc
    19561 gtcaaacatt ctaggatagc agcaggagga aggcatgaaa aaagaaaaac taaaacggtt
    19621 cttggatcaa gacaatgtgg tcggttacgt atttgcttct ccattcatcc taggatttct
    19681 attgataaca gtgataccga tgatcttgtc actttattat tcgttcaccg attatcgttt
    19741 gggaaatcca gtctcatgga tcggcttgga taattatata aagatgctga gtgatgcccg
    19801 ttttttgaac tccatccgag tcacttttct ctatgtattt gcatctgtac ctgtgaaact
    19861 gatctttggt ctattgattg cttatctttt aacgaggaat atcaaaggaa cgactttctt
    19921 tcgttctttg tattatgtgc ctagtttgat cggtggcagt atttctatcg ctctagtttg
    19981 gcgggagttg ttttctaaaa acgggttagt gaatattttc ctggaacgat taggcgcacc
    20041 tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc cctcttgttt tgatgtctgc
    20101 ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt atcaaagaga tcccaggctc
    20161 gtattatgaa gcggcagaga tcgatggagc gaacaaatgg cagacgtttt acaagatcac
    20221 gttacctctt ctttcgccga tcattttgta taacttggtt atgcagacaa tcgcggcgtt
    20281 tatgactttt acgcaggcat ttatcattac ccaaggtggt ccgaatgatg ccacgaactt
    20341 ttatgctctt tatgtgtata accaagcttt ttcttttaat tctatggggt atgctagtgc
    20401 tatttcgtgg atcatgctgt tgattatggg agctgtcacc tttctgattt tcaaatcatc
    20461 aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat gaataataca gtaacgaaat
    20521 ggtttcaata cggttttatt ttgctattag gtctagtcat gatttatccg gtcttatgga
    20581 tgatcgcagg ttcttttaaa acggatcaag agattatgag tggttcgctg aatttgattc
    20641 cagagacttt tcaatgggaa aattacgcaa aaggatgggc ggggtttgct ggaatttcat
    20701 tttttacttt ctttaggaat tcgtttgtca tctctgcagt ttcgacgata gggacagttc
    20761 tgagctcgac ttgtattgct tacgcattat cgagaatcga tttcaaagga aaaagattct
    20821 ggttcgtcgt catgttagta acgatgatga ttcctgcaca agtgattttg attccgcagt
    20881 ttattattta caatcggctg aatttggttg ggacgtatgt gccgctgatt ttaccacatt
    20941 tttttggaca agcatttttc atttaccaga tcatgcaatt catggtcagc atccccaaag
    21001 agttagatga atcagcgatt attgacggat gcagcaagta tagtgtcttt accaaaatta
    21061 tttttccttt gctgaaacct tcgatcatca cgacgatcat catccagttt tattggaaat
    21121 gggacgactt catgggacca ttgatttatt taaacaagcc gagatcttac acggtatcga
    21181 ttgccatcaa attatttgca gacgctggat ctagtacgac ttacgcgagt atgtttgcga
    21241 tgtctacgtt gtcgctgcta ccggtttttc tgattttctt attcttcaat aaatatcttg
    21301 tccaggggat cagtacaagc ggtctgaagg gctgaatatg aaaaagccaa tagaagagat
    21361 gtttctttac aagctcaact actatgtctg tcggattttt ttaaccagtc ttatcagtaa
    21421 tgctcttttc gctttagttc taggatgttt gttcacttta ggattagtgc tgtctgctcg
    21481 tgttgtctat ctgatttatt ttttgatgat gtatccttct tattatctat ctgtccatta
    21541 cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc aaacggatgt gtttcatcat
    21601 tacgttgttt cagctgtttg tcattatttt tgcagttgat tggtatttat tagtgggaaa
    21661 gattggagct tggtatctga ttccttttct attgtgtctg ttgctttttg tatcccatag
    21721 tttttttagt acgctaattg atatctgcaa agaagataaa gcagatagtc ttgtttattt
    21781 gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag gggtttctgt ggtatttatg
    21841 ccttttgtat ggactgttct ggtgtataca gcatgatttt ttactcactg cattactgta
    21901 tccagggctg ccatttgtca cagtattttg cttaaaagaa aaggagaatg actttaaatg
    21961 aagaaaaaat atgtacaagt cggacttggt gggagagcgc gtttttttta tgaggcaatt
    22021 gcctctacgt atcatcaaac ttgtgaacta gtcggttttt gtgatgtatc tcaaacaagg
    22081 atggattatg caaatcggat acttgtgaac aagtatggac agaaaaaagt aaagacatgt
    22141 atgccatcac aatttgagca actactggat gacgtacggc cggctgaagt cattattacg
    22201 tcagtagatc gaacgcatca tgattatatc attcgtgcaa tgaaaaaagg atatgatgtt
    22261 atttgcgaaa aacctctgac tatcgatgag gaaaaagctc aagaaataat aacggtccaa
    22321 aaacagacgg gaagaagcct gcgagtcact tttaattatc gttatgcacc gtatcacacg
    22381 aagataaagg aactgataga aagtgatgtg attggtgatg tgttttctat ccacttcgaa
    22441 tggctgttaa atacgcaaca cggggcagat tatttcagaa gatggcatcg aaataagcac
    22501 aatagcggcg ggcttcttgt tcataaatct acccatcatt ttgatctagt gaatttttgg
    22561 ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat taaactttta tgggatgcat
    22621 aatgcggaga aacgcggagt agaaatattt tacagtcgat gctatggaag cgaactagct
    22681 aaagctgatc catttgctat agacctgtca gccaatgagg aattgcgaga aatgtattta
    22741 gaagcagaaa aagacagtgg atatattcgt gaccgcagtg tattttccga tgatatctct
    22801 attgaagata cactaggcgt aacggtcaaa tatgaaactg gtgcgatttt gacgtattcg
    22861 ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt tcaatggcag caaaggacgg
    22921 atcgaaatga aagtgatcga aaagtcttac atcaatgcag gtggagataa aaaggatgaa
    22981 ggagcagccg ttcttcagtc catccatgtc tatccgatgt tcggtgagcc atatgaggta
    23041 atcgtcgaac aagaagcggg cggacatggc ggaggagatc cgcgtttatt agatgatcta
    23101 tttggtgaag aaaaagaaga agatccgtgg aatcgggcag caactcacgt agatggtatt
    23161 ttgtcgattt tgacaggaat cgcagcaaat cattctattg cttcaggaaa agcagtagtg
    23221 attgacgagc ttgtcagctt aaaagaataa gaatagattt acggaaatta cttcccgtag
    23281 tttataaaat cttgccttat tgccgatagg ctgtcttttg aacactattt attcggaaaa
    23341 aggggctatg acatgctttt tgtcatgcct tctttttatg tgggctgctt ttttagatag
    23401 gagaaaataa aaataactat ttaaacccga acaaaaagga ataatgcatt aaaaatcatt
    23461 agattttaca aaaaaataag taaaccacgt tgtcagtttc agaaagtagt gttagactgt
    23521 tttcaatatt tgaaacaaca ttttttattc aaatatggca aactgccttc acgtgcaaaa
    23581 cagttgcaag aaaatgaatt tacactatta ttagttttac atacctactt atatatcgtc
    23641 ataatatgga tgacagtttc tagccagtac cgtaaatgct ggactataag taaaagattg
    23701 gctatttcac ccttcttttc ttatgataaa gtggagggtg tttttcttta acagaaacat
    23761 agtaggtttt tgcacaaaag cggttatttc ggtattaata aatgataaag gagcaaataa
    23821 agtgaaagaa ttagttgaac gtattcaaaa agacggacgt atattaggcg agggcgtatt
    23881 aaaagtcgac agcttcgtca ctcaccaagt agaccctgta ttgatggaac aaatgggaaa
    23941 acggtttgca gaagtattta aagaacaagg aatcacgaaa gttgtgacca ttgaagcttc
    24001 tgggatcgca ccagctttat ttgctgcaag agaattagat gttccaatga tcttcgctag
    24061 aaaagcaaaa agtttgacga tggatgaaga attattgaca gcttctgttt attcttttac
    24121 gaaacaagtg acaagtacga tttctatttc tcgtaaattt ttatcttcag aagacaaagt
    24181 attgattatc gacgatttct tggcaaacgg acaagcggca aaaggattga tcgaattatg
    24241 ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc atcgaaaagt ctttccaaga
    24301 tggacgtcag ttacttgaag aaatgggatt gagagtcgtt tctcttgcgc gtattgcttc
    24361 cttgacgaat ggacaagttg agtttcttga ggaggacgca taatcgtgga aaaagaaaca
    24421 caaaatggaa aagctgcggt tttagggctg cagcatctgc tagcgatgta tgcaggagca
    24481 gtagcggtgc ctcttttgat cggtacagga ctaggcttta atcaagaaca gatgacttat
    24541 ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat tattgcaatt gacagttaac
    24601 cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg cgatccaagc agtagctcca
    24661 ctgatcttga tcggaaccaa tgaaggagta ggagctatct acggatctat tatcgcttct
    24721 gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa ttaaaaaact attccctcct
    24781 atagtaacag gaacggttat caccgttatt ggtttgactt tgattcctgt agcaatcgag
    24841 aaaatgggtg gaggtaatgc aacagcagct gattttggtg acaaaacaaa tctgctactt
    24901 gcatttgtga cgattctcct aattgtaggc gtgcaaatgc tggcaaaagg gttcattcgt
    24961 tcaatcgcgg tattgattgg tttagtcgga ggaagcatct tagctgcttt catgggaatg
    25021 attgatctga gcgcgattgg agatgcgccg ttgttccatg ttccacaacc cttttatttt
    25081 ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga tcatcatttc catggtcagc
    25141 atggtagaat caacaggtgt atattttgca cttggtgaca ttactgggaa aaatgttgga
    25201 gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg ccgttatctt aggcggtatt
    25261 ttcaatacat tcccttatac tggcttttct caaaatgtcg gattggttca actttcggga
    25321 atcaaaacaa gaaaacccat ctatttctct gcattcttct tgattgtttt aggattattc
    25381 cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg tacttggtgg aggaatgtta
    25441 gtcatgttcg ggatggtggc agtccaagga atccgtatgc tgttggaagt tgatttcagc
    25501 aatgataaaa atctattgat tgttgctgtt tctgttggat ttgggctggg tttcaatgtt
    25561 acgccgacat tattcagtca aatgccgcaa acattgcaaa tgttcactgg aaatggcatc
    25621 gtaatgagta gcattacggc tatcatattg aatttgatct ttaatggatt gaaaaaagat
    25681 gaagaaatat tggaagaagc cttcaagaag taatagagca aaagaaaggt tgtgaaatca
    25741 gcgttttgac ctgattgtta gagaaacaaa cggaaaaata gttcctcgta ttttgccgtt
    25801 tgtttgagct aataccgagg gtcagctgat tgaacaccgt tgaaggaaga ggtcgtgagc
    25861 ctgccgttca tctccgagtc acaaggaaca atttcaaaaa aaagcttctc atgtttgttg
    25921 aatattgttc cttgtgaccg aggagttggc aggagaacac cgtttatcca taaaaaggaa
    25981 tgtgacctaa cttttgtcac attccttttt tttatcttta ataaacacga acaataaaat
    26041 gttttctttg ttaacattcg aaatttcttt gacatcaaaa aaagtgattg ttaaaatatg
    26101 aatgtagaaa aactgggagg aaaaacaatg acagcaaaaa tctctgtgat tatggggagt
    26161 acttcagatt gggaaacaat gaaagaagca tgtcaaatat tggatgaatt cggtgtgtct
    26221 tatgaaaaaa aggtcgtttc tgctcatcga accccagatt tgatgtttga atatgcacaa
    26281 aacgctcgaa aaaaaggaat caaaatcatt attgcaggag cagggggagc cgcgcatctg
    26341 ccgggtatgg tcgcatcaaa aacaactttg ccggttatcg gtgtaccggt ccaatcacga
    26401 acattaaatg gtttggattc gcttctttcg attgtccaaa tgcctggagg agtacctgta
    26461 gctactacag caatcggaaa agcgggagca attaatgctg gactacttgc gatacagatg
    26521 ctttccatgt atgatttaga attggaggct aaattggctg aaagacgttc aatgttggct
    26581 aaaactgtta tagaaagtag tgatcaactt ggttaaacct ttgttgcctg gttcaacgat
    26641 cgggatcgtc ggtggcggtc aattaggtcg tatgatggcg attagtgcaa aagaaatggg
    26701 ctttaaagta ggtgtgctgg atcctgtgag cgattgtcca gccgcacaag ttgcagattg
    26761 gcacattgaa agtgtctatg atgacacctt tgcgctgaaa gagcttgcca gaagaacaga
    26821 tgtcattacc tatgaattcg aaaacgtcag tatagaggcg ttaaatgcga ttttacccat
    26881 gtcatttatt cctcaaggga cagacttgct tgcgattacg caagaccgcc tgttagaaaa
    26941 atcttttttg gaaacgaata acatcgtgat tgctccttat gcaacgatcg tcagtccgac
    27001 ggatatccaa gatgcgattg acgggatcgg ctatccttgt gttttgaaaa ccactcgagg
    27061 cggctatgat ggcaaggggc agtatgtttt gaaaagtcgg gcggatctag ctcctgccat
    27121 ggacctttta agagaaggaa cctgtgtttt agaagcatgg atcccgtttg aaaaagagat
    27181 ttcgatcatg gtggcaggaa atggtcaagg agatttcacg acttttccgg tcgtggaaaa
    27241 catccaccac aacaatattc ttcatgaaac gattgctcca gccgcgattg atcaggatgt
    27301 gatcgaggag gctgagcgta ttgcccgtgt gattgcagaa gcagtgagcc tttcaggtgt
    27361 tttaggggta gaaatgtttt taacaaaaac aggcgggcta tatgtgaatg aattagctcc
    27421 tcgtcctcac aattctggac attattctat tgaagcttgt tctatgagtc agttcgatgc
    27481 ccatattcga ggtatttgtg gttggccatt aggagaaaaa gtacgtttga taagcaatgc
    27541 agtaatggtc aatgttttgg gagaacaact gctggaaagc tatcgcttga ttgagaataa
    27601 gccagactgg caattccatt tctatggaaa aaaacaagca aaaaaaggtc gcaagatggg
    27661 acatatcact atcttgacag aagacatcca gcaaacgctg gaagaaatca aacaaacgaa
    27721 tatttgggat tagaggagaa aaaacatgtt agaacgttat acacgccctg aaatggggaa
    27781 tatctggacg gaagagaaca aataccgtgc gtggctcgaa gtcgagattt tagcagatga
    27841 agcttgggca gaacttggtg aaatcccgca agaagatgtg aaaaaaattc gtgagcatgc
    27901 atcatttgat atcgatcgga tcttggaaat cgaaaaatta acacgccatg atgtcgttgc
    27961 gtttacacga gcggtttctg aaacattggg agaagaaaga aaatgggtcc attatggtct
    28021 aacaagtacg gatgtcgtag atactgctta tggctatctc atcaagcaag caaatgatat
    28081 tttacgggaa gacttgaaac gttttacgga aatcattggc gaaaaagcaa aagaacacaa
    28141 atatacagtg atgatgggtc ggacacacgg tgtccatgct gaaccaacta cttttggatt
    28201 gaaacttgcc acatggtatt cagaaatgaa acggaacatc gaacgatttg aacatgcggc
    28261 aaaaggagta gaagcaggaa aaatctcagg agcagtggga acgttcgcaa atatcccgcc
    28321 atttattgaa gagtatgtat gtaaacaatt ggggatccgt ccacaagaaa tttctactca
    28381 agttctccct cgggatctac atgctgaata tttctcagcg atggcgctta ttgctacaag
    28441 tattgaacgt tttgcaacag agatacgcgg attgcaaaaa tccgaaacac gtgaagtaga
    28501 agaatttttt gcgaaaggtc aaaaaggttc ttctgctatg ccgcacaaaa ggaatccgat
    28561 cggttcagaa aatatggctg gtctagccag agtggtgcgc ggacacatgg tcactgccta
    28621 tgaaaatgtc acattatggc atgaaagaga tatttctcat tcttcagcag aacgaatcat
    28681 cttgcctgat accacgattt tattgaatta catgttaaat cgttttggca atatcgttaa
    28741 aaatctaacg gtctttcctg aaaacatgaa gcggaatatg tctgcaacat ttggtttgat
    28801 ttatagccag cgcgttatgc tgaaattgat cgataaggga atgacaagag aagaagctta
    28861 tgatcttgtt cagccaaaaa ctgcgtacgc ttgggacaac caagtgcagt tccgcccgct
    28921 gttagaagaa gatccaacca tcacatcggt attatctaaa gaagaaatag acgatgcctt
    28981 tgattatcat catcatttga aaaacgttga cgtgatattc aaacgagtag gtttggaata
    29041 aagaagagtg agttgcaaaa ttcaattaaa atagaatagc aaaaataacc aaaaaagaag
    29101 cagatccgtt agcggttctg cttcttttag tttattatat tttcttatca ataataggaa
    29161 cctgatttta aaagcggcta cctctttacc gtgcctcttt acatacaaaa aatagaggaa
    29221 ttaagcgtaa atggcttggc tattataact aaaatgctgg ttatggagat gttgcagcaa
    29281 gttttcgaga aaagcgtatt ccatctcttg tccattgaat aagatctgga tacgataatg
    29341 atttttgaaa cgcttctgtt catgaatctg actgattgaa agttcgcctt tgtgttctgt
    29401 ctgataaaga aaagtccttt tacggcattc agcacagcgc ttggttgctc ggcggatttt
    29461 tcggaatgta taaaaactag tatctttatt gcataaataa acgtctttga tcgacagcaa
    29521 atcattatat agacaggaac gttcttcagc tgttaattct gataaaaaaa gctgaccttg
    29581 ttttattgca tcttccatcg gcaagtgcct cctcgaaccc attatccaaa tttttagtta
    29641 attctaatat accatcatta agtataaatg taaacgcttg ttgatggtat aagattttcg
    29701 tttctgataa aacacgaaca ttaaaagaat ctaatgcctt aaatgtacat atttttgttg
    29761 atttataaaa agatgattgc taaaatttaa agtgaaaaaa cgaacaaaaa acgatgtatt
    29821 tgcttttaat gtaaataaaa aggagagacg tacatggaga agaaagaact tttgtatgaa
    29881 ggaaaagcca aaaaaatata tcttacttct gatgagaact tgatttggat agaatattta
    29941 gatcaagcaa ctgcgttgaa tggcgcgagg aaagaccaga taagtggaaa aggagaattg
    30001 aataatcaaa tcacaagtgc aattttccgt tatctgactc aaagaggaat aaaaaatcat
    30061 tttattgaac aattgtctaa aaccgaacaa ttagcaaaaa aagtacaaat tatccctctg
    30121 gaagtggtta tccgaaatat tgcagcagga agtttctcaa aacgtctagg tgttcccgaa
    30181 ggtaaagaat tcatcaaacc aatcattgaa ttctactata aggatgacca gttggatgat
    30241 ccatttatca atgaagagca tatctatttc ttacaacttg caaagccgga agaaatcagt
    30301 gagctgaaag cccaagcgtt ggctattaat gaaattttga ccgaattatt tgctaaggtc
    30361 ggtttggttt tagtagattt caagctagaa tttggccggt cgaatggaca aattttgctt
    30421 gcggatgaag taacaccgga tacttgccga ttatgggatg aacgtacgaa tgaacatatg
    30481 gacaaagacg tttaccgcag agatctaggt gatttgatcc cagtctatca agaagtatat
    30541 aaccgtctat cggaactaac tgaagaaaag gagcgagtat gatgtatttc gtaaaagtat
    30601 atgtaaccta taaagattca gtgttagatc ctcaagggga ggcagtaaaa aaagcagtcc
    30661 atcgtatggg atatgaaaca atcgaagata tccgaatagg aaaatacttt gagatacaac
    30721 ttgctgatgc agaagaacca atcgatacag tgattgaaac gatttgtgac aagctgcttg
    30781 caaatgtaaa tatggaaaca taccgatatg aaatcaaaaa aatggaggaa gtataacatg
    30841 aaatttgcgg tcatcgtttt tccaggttca aattgtgacg cagatatgtt atgggcagtt
    30901 cgcgagatca tgggcgctga tgccgaattt gtacggcatg atgagtcctc tttagaagga
    30961 tttgatgggg ttttactgcc gggaggattc tcctatggtg actatttgag atgcggtgcg
    31021 attgctcggt tttctacaat catggaagaa gtgattcgtt ttgctgaaga agggaaacca
    31081 gtattcggta catgcaatgg attccagata ttgacagaag caggactttt gccaggtgta
    31141 ctgagacgaa atgagtcttt gcattttatt tgcaaaactg taccattgaa agtcaacccc
    31201 aagacggcct ttacttcaga gtatgaagcc gacgaaataa tctatcttcc agttgctcat
    31261 ggggaaggaa attatttctg tgacgaagaa acacttcaag aactaaaaaa aaataatcag
    31321 attgccttta cttataatga caacatcaac ggcagcgtgg aaaacattgc agggattacg
    31381 aacaaagcag ggaatgtgtt aggaatgatg cctcatccag aacgtgcagt agaagacttg
    31441 ctaggatcag aagatgggaa acggttcttt gcttccattt taaaaaactt cggaaaggtg
    31501 accgtataga tgaaaacatt gaaagaaccg acagctgaag agatcaagaa aaaaagaatc
    31561 tatgccgaat ggggattgac tgatgaagaa tatcgaatga tcgaagaaga tatcttagga
    31621 agaatgccta attatacgga aactggactg ttttctgtta tgtggagcga acattgttcc
    31681 tataaaaatt ctaaaccggt tctacgcaaa tttcctacta gtggacctca tgtgttgcaa
    31741 ggccctggag aaggcgcagg catcgtcgac atcggtgatg gactagcagt tgtcttcaaa
    31801 gcagaaagtc acaatcatcc ttcagccgtg gagccttatg aaggagctgc gacaggagtt
    31861 ggcggaatca tcagagatat tttcagcatg ggggctcgtc caattgcgtt gctagattca
    31921 ttgaggttcg gcgaattaga taatccaaga acgaaatatt tactagaaga agtagttgca
    31981 ggtatcggcg gatacggcaa ttgtatcggc ataccgactg taggcggcga aattgcattt
    32041 gatccttgtt atgaaggaaa cccattagtc aatgcaatgt gcgtaggttt gattgaacac
    32101 aaacatatcc agaaagggca agccgctggc gtcggtaatt cgatcatgta tgtaggtgca
    32161 aaaacaggtc gcgacggtat ccacggagca acttttgcct cagaagaatt taatcaagaa
    32221 gaagaacagc aacgttctgc tgtacaagta ggcgatcctt ttatggaaaa gctgctgctt
    32281 gaagcgtgtt tggaattaat cttgcatcat tcggattctc taataggtat acaggatatg
    32341 ggagcagcag gacttgtttc atctagtgcg gaaatggctt caaaagctgg ctcaggactt
    32401 atattgacat tagatgaagt cccacaaaga gaaactggga tgacacctta tgaaatgatg
    32461 ttatcggaat cacaagaacg gatgttgatt tgtgttaaat caggagagga agaaaaaatc
    32521 caagaactat ttcagaagta tgatctagat gcagtcacta tcggtaaagt aacagacgac
    32581 gggcaatatc ggctatatca tcaaggagaa gaagtagcaa atctgccagt cgatgcatta
    32641 gctgaagatg caccagttta tcataaagaa atgaaagaac cagctcgaat cgtagaattt
    32701 cagcagttag ctccttatca gccagttatc gaagaacctg gacaagtatt gcttgatctg
    32761 ctgcagcaac cgacgattgc ttcaaaacgt tcaatctacg aaacttatga ttcacaagta
    32821 caaaccaata cagttgtccg tccaggaagc gatgctgctg tgatgagggt tagagggacg
    32881 aataaagcgt tagctatgac cacagactgc aacgcgcgct atctgtattt ggatccaaaa
    32941 atcggaggac aaatagcagt ggcagaagct gctagaaata ttattgctag cggtggtaag
    33001 cccttggcga tcaccgactg cctgaactat ggttctcctg ataaaccaga agttttttgg
    33061 gaattatcga cttcagctga cggaattgct gctgcgtgtg aaacgttagg aactcctgtt
    33121 atttcaggaa atgtctcttt atataatgaa acggatggtc aagcaatcta tccaacacca
    33181 atgattggta tggtcggatt gattgaggat cataaacata tcactactca ggaattcaaa
    33241 aaatcagggg atctaatcta tatattaggt aagacgtttg cagattttga tggcagtgaa
    33301 ttacaaaaga tgcagcttgg aagaatcgaa ggtgtgatcc gaaactttga tctatccatt
    33361 gaaaagagaa accaagagtt agtattgaca gccatccaaa acggattgat tgaaagtgct
    33421 catgactgtt cagaaggtgg cttggcaatt gcactggcag aatcagcttt caaacatcag
    33481 ctagggatat ctgtacagtt tgaattgtcc tctgctcaat tatttgcaga aactcaatca
    33541 agatttgtgt tgacagtcgc accggaaaat aagacacgat tcgaagaaat gatgggagat
    33601 gctgctgttt tagcaggtaa ggtgacggat gaagcgataa tcgaaatttc agcaactgac
    33661 ggtcagatca aaatagaaac tgcagtagca agaaagtgct gggaggatgc aatcgtatgt
    33721 cttatgaagt gaagagttta aatgaagaat gcgggatctt cggtgtttgg ggtcatccag
    33781 acgccgctag tgtggcatac ttcgggctcc atagtcttca gcatcgagga caagaaggtg
    33841 caggtatcgt gtccaatgat cgaggaaagc ttaaggggca tcgagatttg gggctgcttt
    33901 ctgaggtatt caaagatcaa agaaaattgc agcagttaac aggagaagct gcaatcggac
    33961 atgtacgtta tgcaacagca ggcaatggaa gtgtggacaa tattcagcct tttctattca
    34021 aatttcatga tcagtcgatc ggcttagcgc ataatggtaa cttgatcaat gctcgttccc
    34081 ttagaaaaga gttagaggaa aatggggcga tcttccattc taactccgat acggagatat
    34141 tgatgcattt gatccgacga agtgaagaaa cagatttttt aaataaatta aaaagtgctt
    34201 tgaatgaagt caaaggtggc tttgcctatt tattaatgac agaaacggca atgattgccg
    34261 ctttggaccc aaatggtttt cgtccattag caatcggaca aatgaaaaac ggagcatacg
    34321 tgattgcctc ggaaacttgt gcattagaag ttattggggc acgttttata agagatgtcc
    34381 agcctggtga gatagtgatc atcaatgacg aaggaatcca gattgattgc tttacgaagg
    34441 acacgcagct ttctatttgt tcgatggaat atatctattt tgccagacca gattcaaaca
    34501 tcgcagggat caatgtgcat actgcacgga aaaatatggg acggaattta gcgaaagaat
    34561 cgccagtaaa agcagatatg gtcgttggcg tacctaattc ttctttgtca gccgcaagcg
    34621 gttatgcgga agctagtgga atcccttatg aaatcggatt agtaaaaaat caatacattg
    34681 cccgaacatt tatccagcca acgcctgagc taagagagca aggtgttcga atgaaattat
    34741 ctgctgtacg aggagtagtg gaaggtaaaa aggtgattat ggtcgatgat tcaattgttc
    34801 ggggtacgac aagcagacgg atcgtccatc ttttgaaaga agcaggggca aaagaagtcc
    34861 atgtgaggat tgcttctcct cctttgaaat atccatgttt ttacgggatt gatattcaaa
    34921 cgcgtaaaga actcattgcg gcaaatcaca cgattgatga aatcaaagaa tgtatcggtg
    34981 cagattcttt aggtttttta agtgaagagg gtctgatcga atcgatcggt ctggaaaaag
    35041 atgctccgta ttcaggattg tgtatggctt atttcaacgg agattatccg acgccgttat
    35101 atgactatga agaaaaatat caggaatcac taaaagaaaa agtatcgttc ttttaaaagc
    35161 agaccaagaa gggagtatat gaattcaatg gcaaatgcat acgcaaaagc tggcgtagat
    35221 gtcgaagctg gatatgaagt agtagaacgg atcaagaagc atgtcaaaaa aacggaacgt
    35281 ttaggtatga tgggcgctat aggaggattc ggcggttgtt ttgatttatc ggtctttcaa
    35341 ttaaaagagc cagtccttgt ctcggggacc gatggcgtgg ggacgaaatt agcagtggca
    35401 atcgaagcag acaaacacga tacaatcggg atcgactgcg tagcgatgtg tgtcaatgat
    35461 atcttagcac aaggcgcaga gccgttatat ttcttagact acttagcaac ggggaaaaat
    35521 gatccgttac gtttggaaaa tgtagtaaaa ggtgtagcag atggatgtgt tcaaggaaat
    35581 atggcgctga ttggcggaga aacagctgaa atgcctggta tgtactcagg taatgattat
    35641 gatctagccg gatttgctgt agggatagct gaaaaatctc aattattgac aggagaaacg
    35701 atcaacccag gagatgtgtt gcttggaata gcatctagcg ggattcattc aaatgggtac
    35761 tctcttgttc gcaaaatttt ctttgagcag cagcagtttt ctttatcatc aaaactgcca
    35821 gaattgaaaa aaacacttgg cgaagaattg cttgagccaa cgaaaatcta tgtttcttcc
    35881 ttgcttccgc tgatcaaaga aaagaaaata aaaggagctg cacatatcac tggtggaggg
    35941 tttgtagaaa atatcccgcg aatgttacca gaatctatgg ctgcacaaat caaacttggc
    36001 tcatggccga ttcttccaat attcggctgt ttacaaaaat atggtgaaat tcctgagatg
    36061 gagatgtatg aaatcttcaa tatgggcatc ggtatggtcc tggcagtcga agaatcgaaa
    36121 gtaaaagaaa tagtacacga aattgagcaa gctggcgaaa aagtttatga aatcgggaaa
    36181 gtgatcgcca aagaaaaaca tcgtgtggtg tttataggag aagaacgatg agaatcgctg
    36241 tttttgcttc tggaaacgga agcaactttc aagcgctagc cgattatcta tcgaaaaaag
    36301 gattggaatc ttcgattgac tggttgtttt gtgatcagcc tgaagcatat gttttgaaac
    36361 gtgcaactgc gttgagtgta ccggcagatt gtttttcacc taaagaattt gattcaaaaa
    36421 aagagtatga agaagcaatt ttgcataaat tgaaagaaaa aaagatcgat ttgatcgtat
    36481 tagctggata tatgcgaatc attggaccgg tattgctgaa aaattatgat aaaagaatca
    36541 tcaatatcca tccgtctctt ttacctgcct tccctggttt gcatggcata cgtgatgcct
    36601 ttgaagctgg agtaaaagaa acgggtgtca caattcatta tatcgaccaa ggtgtagata
    36661 ctggaccaat catccggcag gaaaaagtga gaattgaaca ggaggacacg ttcgattctt
    36721 tagaagaaaa aatccataga gtagagcatc gaatctatcc agaagtgatt agtgaaatta
    36781 ttgaaaatgg aggctatctg aaatgacaag agcattgatc agtgtatcag ataaaactgg
    36841 gattgtagca ttcgcccaag gtttgattga acaaggagta gaaatcattt caacaggagg
    36901 aacaaagcat gcgttggata aagctgggat caaaacgctt tcgatcgaag aagtgacagg
    36961 gtttcctgaa atgatggatg gccgtgtcaa aacacttcat cctaaaatcc atggtggtct
    37021 tttaggaaga cgcgatctcc ctagccatat ggaggcaatg gaaacggaaa atatccaacc
    37081 aatcgacttt gtttgtgtca atttatatcc tttcaaagaa acgattatga aaccagatgt
    37141 cgaaatagca gaagctattg aaaacattga tatcggtggt cctagtatgc tccgtagtgc
    37201 agcaaaaaac catgaatttg tcacctctgt tgttgatcca tctgactatg aagaagtact
    37261 ttcagaatta cgtcagtatg ggcatacttc actttctttc agacaaaaac tagcggcaaa
    37321 agtctttcgt catactgcgg catatgatgc actgatcgct caatacttaa cggattgggt
    37381 ggaagaaaaa gaaccggaaa aattgacact tacgtatgaa cgaaaacaaa cgcttcgtta
    37441 cggtgaaaat agccatcaag ccgctgcctt ttatcagtcc gctcttctgg aaaaatattc
    37501 gatcgcagca gcaaaacaac tccatgggaa agaactttct tataataata tccgagatgc
    37561 agatgctgcg ctacggatca tgaaggaata tgacgagcca acggtagtcg ccttgaaaca
    37621 catgaatcct tgtggtattg ggacagcatc tacgatttta gaagcgtggg aagcagcgta
    37681 tgaagcagat cctgtttcaa tctttggtgg gatcatcgtt ttgaatcgag aagtagatct
    37741 gccaacagca gaacagatgc ataagctgtt tctagaaatc atcattgccc cttcttatac
    37801 acaagaagct ttggaattac tgcaaaaaaa gaaaaacatc cgattactga cagtagactt
    37861 tgcgcaggaa gaaaatcaaa atcgagaaga aacggtctca gtcttaggtg gacttcttgt
    37921 acaagatcaa gatctggcaa tagaaaatga agaagagtgg aaagtcgtga caaaacgtaa
    37981 gccgactcca gcagaagcaa aagcattagt gtttgcttgg agagctgtca aacatgtgaa
    38041 atctaatgcg atcgtactgg caaacgaacg tcagacagta ggaatcggag ccgggcagat
    38101 gaatcgagtc ggttcagtaa aaattgcgat tgaacaagcg caagagaaaa tggaaggtgc
    38161 agttttagct agcgatgctt attttccaat ggatgattcg gttgaatatg cagccaaaca
    38221 tggtataaaa gcaatcattc agccaggagg aagcatcaaa gaccaagatt ctattgatat
    38281 ggcgaataaa tacgatctag caatggtctt tactgatgtt cggcatttta gacattagag
    38341 aggagtcgga taaatgaaca ttttggtgat cggtgcaggt ggtcgtgaac atgcaataag
    38401 taaaaaactt ttagccagtc cgaaggtgga tacggtgtat tgtgcgccag gaaatccggg
    38461 gatgacaaaa gatggtatca attgtgtcgc tatcagtgag cgagatcatt cagcgttaat
    38521 ccattttgtt aagaaagagg ctattgattg gagtatcgtt ggtcctgaag ttcctttgtt
    38581 aaatgggatc gtcgatgatt ttatggcgga aggattgaaa atcttcggtc cgacaaaagc
    38641 agcagctctg atcgaaggtt cgaaaagttt tgctaaacaa ttaatggata cttatcaaat
    38701 cccgacagct aaatccagaa gttttgaatg ttacgaaaaa gcagcagcgt atatcaaaga
    38761 aaatggtgcg ccaatcgtag tgaaagcgga cggcttagca gctgggaaag gtgtagtcgt
    38821 ggctaccaca gtgaaggaag cgctggatgc ggcagaacaa atgttgaacc agcatcgatt
    38881 tggggatagt agcaaaaaaa tcgtcgtaga agagtttttg gcaggagaag aattctccct
    38941 tttggcgttt gtccgtaata ccaaggtgta tccgatggtc atttcgcaag atcataagcg
    39001 ggcatttgat aacgataaag ggccaaatac aggcggaatg ggtgcttatt caccagtacc
    39061 gcaaatttca aaaaagatga ttgaaaaagc tgtggaagaa gttttaaaac cagcggcttt
    39121 gggaatgaaa gaactaggac gctctttcac gggtgtttta tatgcgggtt tgattgctac
    39181 gaaggaagga ccaaaagtga tcgaattcaa tgcgcgtttc ggtgatcctg aaacgcaagt
    39241 cgttctttct cggttgaaga gtgactttgc gcaagtaatc gatgatttat tagagaatcg
    39301 tacagtagag ttaaagtggc agcaagaagg atacaatgta ggcgttgtgg ttgcagctgc
    39361 cggttacccc gaagaatatg agacaggaat ggttctacca gattttctgg aagaagagct
    39421 ttctgtttac tatgctggag tttcctcaaa taacggcaaa ctagttggag caggaggacg
    39481 tctttattta gtagaagcat acggtgagac gatcaaagaa gcacagaaaa aaatttacac
    39541 cgcgttaatg gataaaacga cagatggaac attttatcgt acagatattg gagaaaaagc
    39601 tgccaatagc gaactgatcg tgaagtaaat cagctgcttc catttataaa aaatacaaca
    39661 aacataatcg gaagcgccga ttttagtgaa gccttttcat aaagagtgtg gtacactttt
    39721 gttgtgtata taaataagga ggagttcaga tgaaaaaaat tcaattaggt acttctgatc
    39781 agaaagcagc tgcagtgatc ttaggctgta tgcgtatcaa caatgcggaa gatccagtaa
    39841 aagtgattga gacagcttat gaaaacggca ttgatttttt tgaccatgca gatatttatg
    39901 gaaacggtga atgtgaagaa atctttgcag atgctctagc aaaaacatca atcaaacgag
    39961 aagatctttt tattcaaaca aaatgtggaa tcgttccagg tgtgatgttt gatttttcta
    40021 aaaaacatat cattgaatct gtagaaaaaa gtttgacccg tctgaaaatg gattatgtgg
    40081 atgctttact tcttcatcgt ccagatacat tggtagaacc agaggaagta gctgaagcgt
    40141 ttgatgaatt ggaacggtca ggaaaagttc gttattttgg cgtcagcaat caaaaaccaa
    40201 tgcaaatcga attgctgaaa aaatatgttc gtcaaccatt agtagctaat cagctgcaat
    40261 ttggcttgaa acatactgga atgatcgatc aagggattca tgtcaatatg acagatgaag
    40321 caagtatcga tcatgacggt agtgtacttg attattcccg attgcatgat atgacgatcc
    40381 aagcatggtc accataccag tatggatttt ttgaaggtgt atttatcggg aacgaaaaat
    40441 tccctgaatt aaaccaaaca ttagaagaac tagcagctaa atatcaaacg acaccaactg
    40501 gcttggcttc tgcttggatt ttacgccatc cagccaatat gcaagtgatc gccggtacga
    40561 tgaacgtagg gcgaatcaag gaaatcgctc aagcttcaga aattaatatg tctcgggaag
    40621 attggtacga attgtaccgt gctgctggaa acattttacc ttaaagattg tcataagcag
    40681 gtcaaaaggg ctataaatta aaaagacaga ataacttgaa agctgttttc agcttcgaga
    40741 tattctgtct ttttttattg caaatggaaa gagtatcatt gtacggattt cgtttattag
    40801 ttatttttgt ttttaaggaa aagctgtgtc cgatattctt ttggagaaat cccttgtttt
    40861 ttcttaaata aataactgaa atagttaggg tcattataac caatttcaaa agcaatatct
    40921 gaaatacgat agtctgattg ctgcaataat ttttttgcta attcgattct ttgttctgtc
    40981 agataatcga tgaatgtttg gttaaacgat tgagaaaaaa tcgtactgaa atgagaggga
    41041 cttaatgcaa cttgttgagc gacccaagtt aaggacatat ctggatcggt aaaattttct
    41101 ttgataaaat ccaatgcatg gttcaaaatt ggctgatatt tgttccttga aggctgtgag
    41161 ggcagagcag ttaagaaaga aatgaacata taaactgtcg aacggtaaag cgagtcgtcg
    41221 gcgctgattg ctaagcgttg actggtggat gtgcaatgaa ccatttcttg cgggattttc
    41281 ttttctttca ggcagacatg ctggagtagc ttagtcaaga caaataaacg atataatcgt
    41341 gttcgagttg gtgtgccttg tggtttcgtc aagtgttcga tgtacttaga tacttcgttt
    41401 ttcgataatg aagacaaatc aagtgagagg tcaaagtaaa agacagaatc aactggaagt
    41461 ttcgcttctt gaatcaaatc ctcatagctg ataatttgtt ctttaaggga actggcggtt
    41521 tcaagtatca tctgcgtgat ctcaaaagat gcaggtaatt cgctgattct ttctgcgaca
    41581 ggaccaatgg cgacagaaaa attatttttt tgtccctttt ctaattcatg gaccaacgtt
    41641 tgggctacct gataactggt ctcgatcaca ttttcttcaa gagaatcaac tattaaaaat
    41701 ttgatgaatc gagaggaaac agcagagaag atcacttgtt gattctccct aaataaaaac
    41761 ttcaaataat cactgagatg agcgtaatct tcaaagtctg tactgacttg gtttgctgct
    41821 aataatacgg tcattttttt ccctgctaaa gagcgattga aagattcagc aagtttcaat
    41881 gtttcactga tcgtaagtga gcctttgaac aaaccgttca agaaatgatt ttttttcaat
    41941 tcgaaaacaa gatcagaaga agtgtgcgtt tctttttgct gcaaatctaa agcagttact
    42001 gctttttcta ttgcttgttt caattcgcta cttttaatcg gtttaagcag ataatcatct
    42061 gcctgtacct ggattgctcc tttcatataa tcgaaatcgt cgaatccact aataaatatg
    42121 agacgagtcc aaggaagtag tttccttgct tcttttgaaa aagtcaaccc gtccatgaaa
    42181 ggcatttgga tatcagtcag aatgatgtcg ggctgtaagt cgataattga agacagtgcc
    42241 atctcaccat ctccagcttc gccgacataa gtgattggat aataagaacc aaggacagtt
    42301 atttgttccc gcaagttatt gcgaatgagg tattcgtctt cgatgataaa aactttatac
    42361 atgtttgttt cctccaattg ttttgggaga tagagggatc atcagtgtga cagttgttcc
    42421 ttctcggtat ttgctgtcaa tcgtgatagg tttttgatca tttccgtaat aaagaaggag
    42481 acgcttgttt acgttgtaaa gtccgtagcc gatactgaaa tctgaatcga tccctttggc
    42541 taactcttta cgtatttctg ccagtttttc tgcagggatg ccaatcccgt tgtctataac
    42601 tttgaattgg atcatctcat caattttgca tgtggtaata cgaataaatc ctttgtgacg
    42661 gacgtacttc gttccatggt atacagcatt ttcaatcaat ggctgcaaga tcatttttaa
    42721 aatagaaaca ggaaaaagtg tttctggtat atcgatctta tagtcaagca tatggccgta
    42781 tcttattttt aagatgacta gataatcatt gatatggcta atttcttttt caagaggaat
    42841 ccagtcttgt cctttgctta aagacgtacg aaaaaagccg gaaagtgcat gggtcatagt
    42901 cagagcttct tcaaagcagt cttgttcgat caaagaaata atggcatcca aactgttata
    42961 aataaaatgg ggagtaatct gagcttgcaa tacacgaact tcactttgtg ctaaatggta
    43021 ttgtttcaaa gcgttctccc gtaaagactg gtttagatct tccgccattt tattcatgct
    43081 agcggtaagt gtttgtaatt ccagtgtttc cggcagggtt aaacgaaagc caaggttccc
    43141 tgaagaaagt tgttctgaca tctcgatcag ctgattgatc ggttcttgga cctttctctc
    43201 taaaaagcga cgattctttt tcgcatagaa aaaaatggta ctaagaactc caagttccac
    43261 tattgttaat agaatgagcg agcggacaag tgtcgtattt tttttgcttg ctaagtcgat
    43321 ctccacacga acaaaatctt gtaagatatc tgacagcagt tgagtgacag aatctacctg
    43381 attgatgatc tcttcaatgg tatccataaa atcttttgcc aagatattat tgatcagctc
    43441 gttttgataa tcttccagtg tatcgatcgt acgtagcgat acatctaacg tactaatttc
    43501 tttaagtgta tttgtattat ttttgatctc tcgaatatca ttcctaagtt cttctaggat
    43561 attttcctca ttgaactcat ctacggttac ttggccagaa atcaagtccc acatactttc
    43621 caaaactttt tgatcaacct tcaaagcaat tgcatctgct ttttggatat ttgcgatcgt
    43681 ctgttgataa atcaaaagct gttgcgaata taacagtatg ctcagtagca aagggataac
    43741 agcaaataga agcatggctt tgtttgcttg ggcaaaaatc tgttttatgc ttaaattttt
    43801 tgtcatgata cttctccagt caataatttc tggttgggat gttagctata ttctgctggg
    43861 aaaagacagt ctcggaaata aaaatatctt taggtaatgt ttttcctgtt tgaatatttc
    43921 caaaataacg agtaacggta ttggctacat accagccgac atcaggttta cactcgacta
    43981 cacaattgac gattcctgag cgtagcttct cgatcatatc ttcttgtcca tcaatagtta
    44041 caatgatcaa atcttctcca ggtttgattg atgtttcttt tatcgcagct aaagcaccgt
    44101 gagtcatttc atcactatgg ctgtataata catcgatttc ggataaatca tttgtttcta
    44161 aatatcgttt catctggtct tttcctttca tccgaataaa atcaccgaca attcggtgaa
    44221 caatagtcat cggaggttta tggtcgatgg ttccatcttt ttttagggtt ggtttacgat
    44281 tgattgattc caaaaatcct tcggagcgta gcttggtcgg tgtggagttc tctgaaccag
    44341 caagttctag gattctgatt tcctgtttct ttgaatttgt gaagtgattg ctgacaaata
    44401 aacccgcccg atttccttct gctttgaaac tggggccaat gtgggtgacg aaaagctcgg
    44461 gatcattcgt ttgaatatgt ctgtcgacaa gtatgacagg gatccctgct cgctttgctt
    44521 cttttaatac agagtcccac ccggattctt gcaaaggagt aaaaatgatc atatccactt
    44581 tataggcaat gaacgtccgt atatcttgga tttgccgttc ctgattcatg tagccatttc
    44641 ggtagagaac ttggtaatct tctttttcaa gcgcagtgat aattgattca gtatgctttt
    44701 tccgccagct actctccgtt cctgattgcg aaaaaccaac gactagtggg tcaggtgctt
    44761 cctttgtatt aggtgtacac cccatcattt ggtaagcgat tacaacaagg gctaaaaaaa
    44821 ataaaacacg tttcacttta aatcactcca tctggatttg ttagtactat cttatcagat
    44881 aaaagggaaa atcaaagtca aaaggaatca atcgaaaaaa atccaagaaa cttaaaaaaa
    44941 tccaagacaa aaaaagtaca cctaaaaaat ttaaagtaaa tcccataaaa aaatcaatgt
    45001 taacgctttc tttattagct atactcggtg tatcaattga aatgggggat aaaaaatgaa
    45061 aaacaaattt agaaagattg ttgggactat cgctattttg accgcaagca ttctattaag
    45121 tgcgtgtggc aattcaggat ctgcagatga tagtacgggg tatgtaggaa tcgctatgcc
    45181 gaccaaatca gcagaaagat ggatcgctga tgggaacaac atggtctcgg aattagaaaa
    45241 actgggttat aaaacagatc ttcaatatgg agaagataaa gtagaaaatc aagtagcaca
    45301 aatagaaaat atgattacaa aaggggtaga tacacttgtg attgcttcta tcgatggttc
    45361 ggctttaact gatgtgttag ctaaagcaaa agaagcagat atcaaggtga tcgcctatga
    45421 tcgtttactg atgaattcag aaaatgtcga ttattacgcg acttttgata attttggagt
    45481 aggtgtatcg caagcagctt atattgaaga gcacttagga ttgaaagaag gaaaaggacc
    45541 attcacgatt gaattatttg gtggctcgcc tgacgacaac aacgcactaa tcaattacaa
    45601 cggagtgatg tctgttttac aaccatatat ggacaacggt cagttggtcg tgccatctgg
    45661 acagacgagt ttcagtcaaa tcgctacgct tcgctgggat ggttctacgg cgcaagcaag
    45721 gatggataat ctgttaagcg caaattatac tgaccaaaca ttggatgctg ttttgtcgcc
    45781 atatgatcca ataagtttag gaatcatttc gtcattaaaa ggcgtggggt atgggagtga
    45841 aagtaaacct ttacctgtga ttactggaca agatgcgaca gttgctggcg ttaaatcgat
    45901 cattgccggg gaacaaacac aaacgatctt taaagacaca cgtatcttag caaaaaatac
    45961 gattgaaatg atcaaagcaa tcagtgatgg agaagaggtt ccggtcaatg atactgaaac
    46021 ttatgataat ggggtgaaaa ctgtccctac gtatttagca aatacagtat ctgtcgataa
    46081 agataattat caagcagagc tgatcgacac cgattattat aaagaatccg atctgaaaaa
    46141 ttaagaggaa ccaaacaagg aactaaagag atctaagtca gagagaggat aagaattatg
    46201 gcggattgta ttttaaaaat ggcagatatc accaaaaaat tttctggaat ccgagcgtta
    46261 gattgcgtga atctgatgat tgaacgtggg gaaatccatg cattgtgcgg agaaaacgga
    46321 gcgggaaaat caacattgat gaatgtcttg agcggcttgt atccatatgg ctcctatgac
    46381 ggggaaatct tttatgatgg aaagctgtgt caatttaaag atttgaaaga tagtgaagaa
    46441 aaaggcatcg tcatcattca tcaagaatta gcactaagtc cttatctttc aataaaagag
    46501 aatatttttc tcggaaatga acaagcagaa agaggcgtga ttgattggga actgacagaa
    46561 aagaaaacat atgaactgct gaataaagtc ggtttgtcta tcgacccgaa tcttctagta
    46621 tcccagattg gcgtagggca acagcaacta gttgaaatag ctaaagcatt atcaaaatcc
    46681 gtccgtctgt tgattttgga tgagcctacc gctgcattga atgaagaaga aagcgcgaat
    46741 ttgctgagat tgatccgaga gttgaaagag caaggtgtaa cctccatcat tatttcacat
    46801 aagctgaatg aaatcgtgga tatcgcagat cgaatcacca ttttacggga tggacagacg
    46861 attgagacac tagaaaatga aatgatcagc gaagagcata ttattcgcgg aatggtgggc
    46921 agagatttag ttgatcgtta tcctgaaaga aaaccggata ttggagaagt ttattttaaa
    46981 gtagaagact ggacggtcta tcaccctctt gatcacgagc gaatcataaa cgaccacatc
    47041 aacttgcatt tgaaaaaagg agagattgta ggaatagctg gactgatggg agcgggcaga
    47101 actgaattcg ctatgagtct atttggacat tcttatggaa gcaagatatc cggacgtata
    47161 ttaaaggaag gggcagagat atcagttaag accgttcctt cagccattgc aaacggactc
    47221 gcttatgtct cggaggaccg aaaatcttta ggactgaatc tgctgatgga tattcgggaa
    47281 aacgtgacgc tgagcagttt ggataaaata agtcaaaaag gagtattaga taaagaaaaa
    47341 gaagtaactg aggcagaaaa tttccggaaa aagatgcgta taaaaacaaa ctccatccat
    47401 caaattgtca gtagtcttag tggtgggaat caacaaaaag tcgttttatc caagtggctg
    47461 atgacacaac ccgatattct ttttttggat gagccaaccc gtgggataga tgttggggca
    47521 aaatatgaga tctacacgat tatcgaagaa atggcagcaa atggcaaagc tgtctgtctt
    47581 atctcttctg aacttcctga aatattaggg atgtgtgatc gaatctatac gatgaacgaa
    47641 ggacgtatta caggagaagt ctcgcgagaa gatgcagatc aggaagtctt gatgaaatta
    47701 atgacgaaag aagaggtggg ttaagttgga acaggcaaaa aatcttacgg ataaaaaaga
    47761 gaaaaaacta aatttgaaaa gtagagcagc tgatcttttt ggtcgctaca gcatggtgat
    47821 cattttagcg ggactactta ttgcgtttca attgatgacg gatggtattt tctggcgtcc
    47881 attgaatata actaaccttg ttctgcaaaa tagccatata ttagtactag ctgcagggat
    47941 gttattagtt gtgctcttag gtcatgtgga tttgtcggta gggtcagtga tggcgtttgt
    48001 tggtgcaatt gcgggcatga tgatggtcaa tcgccaaatg aacccgtgga tgacagttat
    48061 cgcatgtctt gcagtaggcg ggctgatcgg cgcatggcaa ggtttttggg tagcttatgc
    48121 agggataccg gcgtttatcg tgactttagc aggattgctg atgtttcgtg gactgacaca
    48181 agttgttcta ggaggccaat ctctagcacc ttttccagct gttttccaaa aaatgtctac
    48241 gggttattta ccagacttaa gcgaaggaag cagccttcac ttcttgacat tgattttagg
    48301 aatgatcatc acgatcgtgt tagttgttgg tcaatggcga acaagaaatc gtcggaaaaa
    48361 tcatttattt gaagtggaat ctccaacagc tttcttggta aaggcggtat ttacagcagt
    48421 cgttgtacta ggaataactt atatctttgc ctcttatcaa gggtatccga ttattttgat
    48481 cattcttggt ttgatcgttg cagcatatgg gtttttgaca aataaaacag tagcaggtcg
    48541 acagatttat gcgacgggcg gaaatcgaaa agcggctgaa ctatcaggga tcaaaacaaa
    48601 gcgaatcacg ttctgggtat tcgtcaacat gggagtcatg gcagctttag ctggattagt
    48661 tttagctgct cgattgaatg cggcaacacc tcaagcaggt acatcgatgg aactggatgc
    48721 aatggcggca gtatattttg gtggtgcttc gacttccggc ggcattggaa caatcatggg
    48781 tgcgattgtc ggtggcttgg tcatgggtgt attgaacaat ggcatgtcga ttttaggcgt
    48841 cggggtagac tggcagcagg caatcaaagg attgatcttg cttttagccg ttgtgctaga
    48901 cttgtataat aagaaaaaac gaacaacgta ggagaacgcc ggttattcag aaaaaagact
    48961 gtgacaagac atttgtcaca gtcttttttt cgctaaaagc gaacattcat tgtttcgttt
    49021 gtatagacta aaaataatat atgttttttt accattcgta taaattctct ttttgctctt
    49081 gatttttttg cttaattgag ttaaattaca tagggcgcta aaaaaagcct tgattaaatt
    49141 aataatgttc gtgttttgct ggatgtgaac atcaggagga taatgtaatg aaagtatttg
    49201 attacgaaga tattcaatta attcctaata aatgtatcgt aaatagccgt tctgaatgtg
    49261 atacaacggt cacattaggt aaacatacat tcaaaatgcc agtagtgcct gccaacatgc
    49321 agacgattat tgatgaaaca atcgcagaat tcttagcgga aaatggttat ttctatatca
    49381 tgcatcggtt tgatgaagca gctcgaattc cgtttatcaa aaaaatgaaa aagcgtggat
    49441 tgattacctc gatcagtgtt ggcgtcaaga aagaggaata ttcctttata gagaagttgg
    49501 cagaagaatc gctaaatcct gattatatca ctatcgacat cgcgcatgga catgctaact
    49561 cagttattga tatgattcag catattaaaa aatatttgcc tgaaactttt gtgattgccg
    49621 gtaatgttgg tacaccggaa gccgtccgtg aattggaaaa tgcgggcgca gatgcaacaa
    49681 aagtcgggat tggtccagga aaagtatgta ttacaaaaat caagacaggc tttgggactg
    49741 gaggctggca actggcagct cttagatggt gtgcgaaagc agctcgcaag ccgatcatcg
    49801 ctgacggtgg gatcagaaca catggcgata ttgcaaaatc tgtccgtttt ggtgcgacaa
    49861 tggtcatgat tggttcattg tttgccggtc acgaggaatc tcctggcgaa acaaaagtcg
    49921 aaaatggaat cgtgtataaa gaatactttg gttctgcttc agaattccaa aaaggcgaaa
    49981 aacgaaatgt tgaaggaaag aaaatctgga tccaacacaa aggatctttg aaagatacac
    50041 tagtggaaat gcaacaagac cttcaatcat ctatttctta tgctggagga agagatctgg
    50101 aagccattcg aaaagttgac tatgtcatcg tgaaaaattc gatttttaat ggagacacaa
    50161 tctaaaaaat aaataaaata ttaggatgtt gtgaaaaagt tgtcctgcat taagtaataa
    50221 gaacagccaa acaaaaatag ttctttatat gttttgtttg gctggaatta ttatcgcaga
    50281 tacaagcttt tgaacatcat tattcagaat aagggactgt gataagattt ttgtcacagt
    50341 cccttatgtt ttgcaaaaaa agtttttctt ttttctcgta aattcggtaa aatcaaaaga
    50401 aaaaatagac aatagaaaag agaagcttat gtgggaaaaa gtagagaaaa aacaaggacc
    50461 gatttatcgt caaattatgg aacagattat gagagaaatc gaaaacggaa ggatcaatcc
    50521 tggaagtcaa cttgaatcag aacgaaaact agggcaactt tttggtgtga atcgttccac
    50581 cgttgtccat gcattggaag agcttaggga gcttggggta ctaacgagca aacgtggcag
    50641 cggatggttt gttaatcaga ctgaatgggg gaaattcgct gttccaagaa tcgattggcg
    50701 gcaaatgatc tctcctcgtt atgagcagac ggactggtat gagcaaaaaa taagagaagc
    50761 aaagcagatc caaaaaacat cgtttcttga tttatacgct agcgagatgc cagatgagct
    50821 tttgccgaat tttgagtttc cttcttattc tcttgaagaa atcttagcgg aagaaaaaga
    50881 aatggatctt ttaggctata ctccgttacg taacaaaatc aaacggtatt tggaaagtaa
    50941 atttgaattg gcattttccc ccaaccagct tcttgtgact ggtggcggtc agcaagcaat
    51001 ctttttgatt ttacaaacga ttctttcagc tggagagggg atagctgtag agtcaccttc
    51061 ttttttttac cgattacctc ttttcaaagc ttcaggaacg cgtttatttg ggataccgat
    51121 ggatgaagaa gggatcgact tagaaaaact gaaagaatcg atccataaaa ataaattgaa
    51181 agcagtgctt atcaacccga attttcaaaa ccctacagga aaagtgatga gtcaaaaacg
    51241 acgagaaaag ctagtagaat tgtgtcgaca gtatcggctt ccaattattg aagacgatgt
    51301 atttacggat ctttcatttc taaaccaaga agaagtcctt cctatacgaa cgattgatcc
    51361 ggataatgtg ttatacgtcg gttcgttatc tagggtttta ggaaaaacaa cgaaaatcgg
    51421 ttgggtgata ggacctgaga cttttatctc gcaactagct caagcgcaag aaatgatgga
    51481 gttttcgatg aatatcctta cacaaatcac agtggctaat gtatttaatg aagcaattga
    51541 tttgaaaatg caagatgtaa gaaaaaagtt gaaagaaaaa agccgactga taagcgcttg
    51601 gggagattca caatcgtttt tcaagcttca ttcaatttta ggtggttact atgcctggat
    51661 tacttgggat gggaaaaaac tagaccccga actagcagaa aaagtagtag atatgggtct
    51721 tggaattgca ccaagtctac tttttgggca agatataaat ggaatcagaa tcaattacag
    51781 taggctggat gaaaaccaaa taccgttgtt ttattcaaag atgaaaattc tagaaaagtg
    51841 gttaacagaa taatttcaca aagtaaatag attaaagaat ataacagctt ggatttaaat
    51901 ttcaagctgt ttcttttttt tcggaaaaag aatacgatat aaagaaacta agaaaaaacc
    51961 attatttgta cgtccgttca cgatgtgtaa ataaaataaa tgtttgcaac aaaactgtat
    52021 aacaccaacg tttttaaata gaaacagatg gttgtaagtt tgattagaaa atagaaaaaa
    52081 tgcagatgaa atataaaacg ctttccttat tgtgatttta ttcactaatg ctgttaatat
    52141 gcggattggc ataatttcct ttcatcgtga aaatttgtgc ttttagcttg tatttctcat
    52201 aaatccatga taatttatta atgaagacgg ttaaatagtg atacagtttt attcactgtt
    52261 tcaatcgcag aatgaagaga ttttcttata gatgagagag gaatgtgtga caaatggcaa
    52321 acaaaaaagc accgatcgac ttccaagcac tgctagatga agtcaatgca gatttcccag
    52381 tatatcaagc acttgatcaa gatggaaacg tagttaacaa agatttagta cctgatttat
    52441 ccgatgatga actcgttgaa ttaatgactc atatggtatg gtctcgtgta ttagaccaac
    52501 gttcaacagc gctgaaccgc caaggacgcc taggcttttt tgcaccaact gctggacaag
    52561 aggcaagtca gctggctagt gcttacgcat ttgataaaga agatgttctt cttccaggtt
    52621 atcgggatgt acctcaacta gtaaaacatg gtttaccttt atctcaagct tttttatggt
    52681 ctcgaggaca tgctgcaggt aattcttatc cggaagaatt gaaagctcta ccaccacaaa
    52741 ttatcatcgg tgcacaatat gtacaagcag ccggtgttgc attaggtttg aagaaacgaa
    52801 acaagaaaaa tgtcgtcttc acttatactg gtgatggcgg ttcatcacaa ggtgattttt
    52861 acgaagcaat caatttcgct ggcgcttacc atgcaaatgc cgtattttat atccaaaaca
    52921 acgggtatgc gatctctaca cctcgtgaaa aacaaactgc agcaaaaaca cttgcacaaa
    52981 aagctgtcgc agctgggatt cctggtattc aagtcgatgg aatggatccg cttgcagtat
    53041 atactgtatc taaaatggca cgggaatggg cgatcagtgg aaatggacca gtcctaatcg
    53101 aaacactgac ttatcgttat ggaccacata cactatctgg tgacgatcca acccgctacc
    53161 gtacaaaaga cacagatgac gattggcaga aaaaagatcc attgatccgc ttccgtaaat
    53221 atttgacgga aaaaggactt tggtctgaag aaaaagaaga acaagtcatc gaagcaacta
    53281 aagaagaaat caaagcagct attgctgaag ccgacaaagt accaaaacaa aaggtttcag
    53341 atttcttgaa aaacatgttc gaagtatctc cacaatcaat caaagaacaa attgcaattt
    53401 atgaagcgaa ggagtcgaaa taatcatggc acaaaaaaca atgatccaag caatcacaga
    53461 tgccttagca cttgaactag aaaatgacga gaatgtcgtt gtttttggtg aagatgttgg
    53521 gaaaaacgga ggagttttcc gggcaactga aggattgcag gaaaagttcg gagaagaccg
    53581 agtattcgat actcctttag ctgaatctgg aatcgcagga ttgagtttcg gtcttgcgtt
    53641 agaaggattt cgtccagttc ctgaaatcca attctttggt tttattttcg aagcaatgga
    53701 cgaagtagta gctcaaatgg ctcgtacaag atatcgaatg agcggcacta gaaatctacc
    53761 catcacaatt cgttcgccat ttggtggcgg tgttcataca ccagaattgc attcggataa
    53821 tttagaaggt ttgatcgctc agtctcctgg tattcgagta gtgattcctt ccaaccctta
    53881 tgatgcaaaa ggactattga tttcttctat tagaagtaac gatccggttg ttttcctaga
    53941 acacatgaaa ctatatcgct cattccgaga agaagtgccg gatgaagctt acgaagttcc
    54001 tttggataaa gcagcagtta ctcgtgaggg aacagatgta tctattatca catacggagc
    54061 aatggtgcgt gaagcaatca aagcagcaga caatcttgca aaagaaaata tatctgttga
    54121 aatcattgat cttcgtacag ttgcgccatt agatgtagaa acgatcattc aatccgtgga
    54181 aaaaactggt cgcgtagttg ttgtccaaga agcacaacga caagcgggcg ttgccgcgca
    54241 agtagtttct gagatctcag aacgcgctat cctttcatta gaagccccaa tcggacgagt
    54301 ttctgcacct gatacagtct tcccatttgg acaagcagaa aatgtctggt tgccaaatgc
    54361 aaaagatatc gaagacaaag taaaagaaat cgcggaattt taagaaaaga agggacgaaa
    54421 caaagtcatg gcttatcaat ttaaatt
//
LOCUS       DACCDF010000009        53700 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCDF010000009 DACCDF010000000
VERSION     DACCDF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.009"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..970
                     /locus_tag="IVK51_000508"
     CDS             83..970
                     /locus_tag="IVK51_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6430128.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTITSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IVK51_000509"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IVK51_000509"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP6430129.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IVK51_000510"
     CDS             2272..2718
                     /locus_tag="IVK51_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6430130.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IVK51_000511"
     CDS             2991..4343
                     /locus_tag="IVK51_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP6430131.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IVK51_000512"
     CDS             4514..4801
                     /locus_tag="IVK51_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430132.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5027..5470
                     /locus_tag="IVK51_000513"
     CDS             5027..5470
                     /locus_tag="IVK51_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6430133.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5476..6660)
                     /locus_tag="IVK51_000514"
     CDS             complement(5476..6660)
                     /locus_tag="IVK51_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP6430134.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6928..7626
                     /locus_tag="IVK51_000515"
     CDS             6928..7626
                     /locus_tag="IVK51_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP6430135.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7633..8310
                     /gene="nth"
                     /locus_tag="IVK51_000516"
     CDS             7633..8310
                     /gene="nth"
                     /locus_tag="IVK51_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP6430136.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8436..8969)
                     /locus_tag="IVK51_000517"
     CDS             complement(8436..8969)
                     /locus_tag="IVK51_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430137.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9266..11647)
                     /locus_tag="IVK51_000518"
     CDS             complement(9266..11647)
                     /locus_tag="IVK51_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6430138.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK51_000519"
     CDS             complement(11637..12284)
                     /gene="recU"
                     /locus_tag="IVK51_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP6430139.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12348..12887
                     /locus_tag="IVK51_000520"
     CDS             12348..12887
                     /locus_tag="IVK51_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP6430140.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK51_000521"
     CDS             12977..13396
                     /gene="gpsB"
                     /locus_tag="IVK51_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP6430141.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13418..13785
                     /gene="rnpB"
                     /locus_tag="IVK51_000522"
     ncRNA           13418..13785
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVK51_000522"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13994..15160
                     /locus_tag="IVK51_000523"
     CDS             13994..15160
                     /locus_tag="IVK51_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP6430142.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15206..16702
                     /locus_tag="IVK51_000524"
     CDS             15206..16702
                     /locus_tag="IVK51_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP6430143.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16783..17466
                     /locus_tag="IVK51_000525"
     CDS             16783..17466
                     /locus_tag="IVK51_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6430144.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18111..19301
                     /locus_tag="IVK51_000526"
     CDS             18111..19301
                     /locus_tag="IVK51_000526"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP6430145.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19403..20356
                     /locus_tag="IVK51_000527"
     CDS             19403..20356
                     /locus_tag="IVK51_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP6430146.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20425..21774
                     /locus_tag="IVK51_000528"
     CDS             20425..21774
                     /locus_tag="IVK51_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6430147.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21957..22280)
                     /locus_tag="IVK51_000529"
     CDS             complement(21957..22280)
                     /locus_tag="IVK51_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430148.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22409..23572
                     /locus_tag="IVK51_000530"
     CDS             22409..23572
                     /locus_tag="IVK51_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430149.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23642..24991
                     /locus_tag="IVK51_000531"
     CDS             23642..24991
                     /locus_tag="IVK51_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6430150.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25036..25467)
                     /locus_tag="IVK51_000532"
     CDS             complement(25036..25467)
                     /locus_tag="IVK51_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430151.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25855..28497
                     /gene="alaS"
                     /locus_tag="IVK51_000533"
     CDS             25855..28497
                     /gene="alaS"
                     /locus_tag="IVK51_000533"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP6430152.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28697..29293
                     /locus_tag="IVK51_000534"
     CDS             28697..29293
                     /locus_tag="IVK51_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6430153.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            29415..30563
                     /locus_tag="IVK51_000535"
     CDS             29415..30563
                     /locus_tag="IVK51_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6430154.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(30601..31014)
                     /locus_tag="IVK51_000536"
     CDS             complement(30601..31014)
                     /locus_tag="IVK51_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP6430155.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            31093..31524
                     /locus_tag="IVK51_000537"
     CDS             31093..31524
                     /locus_tag="IVK51_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP6430156.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(31623..31856)
                     /locus_tag="IVK51_000538"
     CDS             complement(31623..31856)
                     /locus_tag="IVK51_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP6430157.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            32039..33709
                     /locus_tag="IVK51_000539"
     CDS             32039..33709
                     /locus_tag="IVK51_000539"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP6430158.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            33856..34242
                     /locus_tag="IVK51_000540"
     CDS             33856..34242
                     /locus_tag="IVK51_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430159.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            34229..34927
                     /locus_tag="IVK51_000541"
     CDS             34229..34927
                     /locus_tag="IVK51_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6430160.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            34932..35396
                     /locus_tag="IVK51_000542"
     CDS             34932..35396
                     /locus_tag="IVK51_000542"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP6430161.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            35393..36304
                     /locus_tag="IVK51_000543"
     CDS             35393..36304
                     /locus_tag="IVK51_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6430162.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK51_000544"
     CDS             36539..37078
                     /gene="pyrR"
                     /locus_tag="IVK51_000544"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP6430163.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            37092..38375
                     /locus_tag="IVK51_000545"
     CDS             37092..38375
                     /locus_tag="IVK51_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP6430164.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            38388..39314
                     /locus_tag="IVK51_000546"
     CDS             38388..39314
                     /locus_tag="IVK51_000546"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP6430165.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            39333..40613
                     /locus_tag="IVK51_000547"
     CDS             39333..40613
                     /locus_tag="IVK51_000547"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP6430166.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            40618..41694
                     /gene="carA"
                     /locus_tag="IVK51_000548"
     CDS             40618..41694
                     /gene="carA"
                     /locus_tag="IVK51_000548"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP6430167.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            41699..44884
                     /gene="carB"
                     /locus_tag="IVK51_000549"
     CDS             41699..44884
                     /gene="carB"
                     /locus_tag="IVK51_000549"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP6430168.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            44938..45714
                     /locus_tag="IVK51_000550"
     CDS             44938..45714
                     /locus_tag="IVK51_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP6430169.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            45711..46643
                     /locus_tag="IVK51_000551"
     CDS             45711..46643
                     /locus_tag="IVK51_000551"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP6430170.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK51_000552"
     CDS             46640..47356
                     /gene="pyrF"
                     /locus_tag="IVK51_000552"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP6430171.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            47363..47992
                     /locus_tag="IVK51_000553"
     CDS             47363..47992
                     /locus_tag="IVK51_000553"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP6430172.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            47980..48294
                     /locus_tag="IVK51_000554"
     CDS             47980..48294
                     /locus_tag="IVK51_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430173.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            48332..48973
                     /locus_tag="IVK51_000555"
     CDS             48332..48973
                     /locus_tag="IVK51_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6430174.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            49004..49708
                     /locus_tag="IVK51_000556"
     CDS             49004..49708
                     /locus_tag="IVK51_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP6430175.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            49705..50607
                     /locus_tag="IVK51_000557"
     CDS             49705..50607
                     /locus_tag="IVK51_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6430176.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(50765..52471)
                     /locus_tag="IVK51_000558"
     CDS             complement(50765..52471)
                     /locus_tag="IVK51_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP6430177.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            52661..53284
                     /locus_tag="IVK51_000559"
     CDS             52661..53284
                     /locus_tag="IVK51_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430178.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT        17594 a         9321 c        11518 g        15267 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaat cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccctttttt tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa
     4981 taggaaacaa aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca
     5041 actgctaata gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga
     5101 aatggaaaaa gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg
     5161 gaaaatccct gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc
     5221 catcagtaag ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga
     5281 agagaataaa aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag
     5341 acacgagcaa ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc
     5401 aaaagaagaa tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag
     5461 tagcaaatga agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa
     5521 tccgaatacc gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc
     5581 gatgacgcgc gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt
     5641 atctgagaca gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa
     5701 accataacag ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt
     5761 gatgaataga taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga
     5821 aaattttttg agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat
     5881 caaagagaag cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg
     5941 agaaaagata gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa
     6001 aggaataacg gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga
     6061 gaaacttaaa tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc
     6121 ataaacggca tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa
     6181 ctcagctaat agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc
     6241 catccatcca aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata
     6301 taaggtggag atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca
     6361 tatcgaattt cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat
     6421 aaggatcgga tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa
     6481 ggcagctaga gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga
     6541 gtgagaataa ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc
     6601 agtgaataag acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat
     6661 gtaaaaactc ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt
     6721 gtttccttgt caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag
     6781 tgaagatacg catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg
     6841 accgatcagt gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt
     6901 atactgtgat tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga
     6961 caaacagtca tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa
     7021 gaatttttat ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat
     7081 ttgcagatga ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat
     7141 cgtctcgtaa caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca
     7201 gatcgatata atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa
     7261 aaaaaatacg aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa
     7321 caagaatttg gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa
     7381 gaagaccatt atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag
     7441 gcttatagtt ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt
     7501 acaaaacaac aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa
     7561 gtagaacagt cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag
     7621 gagtagcgga atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga
     7681 atgtttcccg aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca
     7741 gtgatcttaa gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt
     7801 gcctcttttc cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa
     7861 ataaaaacga ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag
     7921 ctgattgaac gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct
     7981 ggtgtaggca gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc
     8041 gcagtagata cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct
     8101 acggtcatgg aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag
     8161 acacatcata cactgatttt ttttggtaga tatcattgca cggcaagaaa tcccaagtgc
     8221 gaggtatgtc ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa
     8281 aaggcgctaa aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta
     8341 tgaattatct tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt
     8401 aaatgtacat actcagtagc taggatggta acttattaca atttattgcg ataaaaataa
     8461 aagttgttat ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct
     8521 ataaaataag tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt
     8581 gtaataaaaa tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg
     8641 gatttgggcg gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa
     8701 aaagtgagaa tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt
     8761 aaaagtacag aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat
     8821 aataaacaaa atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa
     8881 aaaagatggt gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag
     8941 ccttctttca aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg
     9001 ggattattaa gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata
     9061 aacaagtaaa tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta
     9121 agctaaatgt tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac
     9181 agcttggtac atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac
     9241 gcttgtagac gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg
     9301 gtgtatggtc atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag
     9361 gagcagatga agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag
     9421 acgctggctc gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc
     9481 tttgactagt agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct
     9541 ggacatagct ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat
     9601 attgatcttt gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt
     9661 ctgtatttgt cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta
     9721 cgtgtgacga ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa
     9781 tcgaataatt tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac
     9841 caagttttgc atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata
     9901 acccagcgat ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg
     9961 tgatcatata agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt
    10021 ctgttccgtc ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat
    10081 tggcgaaagc cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat
    10141 cttgttcttc cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc
    10201 caagattttt taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg
    10261 ctggaacatt acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat
    10321 tatcccagtt tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag
    10381 tttttccagt agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca
    10441 tcgtagaacc aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt
    10501 ctgcgatgtg acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg
    10561 tggaggcaac ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat
    10621 catatagttt cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt
    10681 agacattctt atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga
    10741 cgatcttagt attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat
    10801 taacagcttg atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa
    10861 cagtatctcg tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg
    10921 cttgaggcat cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac
    10981 caaagtacat ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat
    11041 ttacgtagta ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc
    11101 acgcttcttg ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa
    11161 gtttgatcaa ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag
    11221 agagggcaga accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat
    11281 cttctaccga cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt
    11341 cacgcttttc tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag
    11401 atacggtagc gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga
    11461 aaagtcctac acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct
    11521 tgataaataa tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct
    11581 tcttcacact ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat
    11641 ttgccattct tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc
    11701 aaataaggaa tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa
    11761 gataaaggta gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga
    11821 acactattca aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc
    11881 ccattttgtt cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag
    11941 ggaaaagatg ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag
    12001 acgccattat agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca
    12061 cttctcttag gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc
    12121 gccaacccgc gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc
    12181 ccccgattgc cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag
    12241 gcattcttat aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct
    12301 aggccacatt atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa
    12361 aagtaatcta tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc
    12421 ctaaaatcca agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag
    12481 ggctggaatg ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag
    12541 tagcggaatt gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg
    12601 attttggtaa taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag
    12661 cagattatgt cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc
    12721 acactcgttt tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc
    12781 ctggaaaaac gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg
    12841 aaattcggct gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt
    12901 tgatttatca acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa
    12961 aactagaggt gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga
    13021 attcaaaaca aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat
    13081 catcaaagat tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg
    13141 attgagtgca aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca
    13201 acaaacagag gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc
    13261 aaatcttgaa cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc
    13321 agcacaacct aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa
    13381 aacacgtcaa ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga
    13441 cttgatcagg ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc
    13501 ttagcgaaac cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct
    13561 tcggctatgc ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat
    13621 ccggtggaac gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt
    13681 gtctggaaga agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg
    13741 ttttttctcc ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag
    13801 ggaattaggt cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta
    13861 tttttcaata cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta
    13921 ttcggaattt gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat
    13981 aaggagattg tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg
    14041 gctagaagca ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa
    14101 cggacgtgca cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg
    14161 tacagcagat cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt
    14221 gtttgaaaac gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc
    14281 tgtagctgga aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac
    14341 aaaaaaagca atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact
    14401 gacggaaact ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt
    14461 aactctggat acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg
    14521 agcaccattg aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga
    14581 ccgtccattt tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat
    14641 aggccataat atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt
    14701 cagtcaagaa atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat
    14761 tgaattagat attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa
    14821 tgcagaagaa atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt
    14881 caaaacggat aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg
    14941 agaagaagaa actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa
    15001 aacatggagt aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa
    15061 agcaaccaaa aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg
    15121 gggaactcgt ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc
    15181 aattggatgt ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga
    15241 aatcaatctt ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc
    15301 tgaaaaagct agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc
    15361 aaaaaaaatc ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga
    15421 attgtcggaa gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca
    15481 tcgcgtaccg gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca
    15541 atggcaaaaa gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa
    15601 tattgaatta acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga
    15661 tgttttgctg aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga
    15721 tcagcttaaa gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc
    15781 tgaaacagac tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa
    15841 agtaatcgaa caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc
    15901 atttatgact ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga
    15961 tttcaaaatg gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga
    16021 catcgatgat aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga
    16081 atcacaatcg ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca
    16141 atatcctttc tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt
    16201 ttatcgttca ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac
    16261 ttaccctctc catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt
    16321 agatgttgct gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg
    16381 accaacaaat gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg
    16441 ttattttccc tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca
    16501 gcaagaaata gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg
    16561 gatgtcagag catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga
    16621 tgcaacacat gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt
    16681 taatgtatat caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct
    16741 atagatgttt tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg
    16801 atcgctgatt atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat
    16861 accaaaagag ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc
    16921 tttgtgacgc ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat
    16981 gagaacgagg caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa
    17041 cagatctcga tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa
    17101 gcggccgaac tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta
    17161 ggagcaatcg gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt
    17221 gatcctgaac tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca
    17281 gtgatcaatc atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac
    17341 ggatatgaag aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt
    17401 ctggaatggt ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa
    17461 aaatgatgga ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta
    17521 aaagttactt ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt
    17581 cacaaggaac aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt
    17641 cggaattggc aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc
    17701 acagtccttt tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt
    17761 aaaaattgct gataaacgaa gatggaaatt ttatgtattt ttattggtct atgcaataaa
    17821 taaattaaat attcagaata caattgattt taacgaaagg gcttctatca atggttgtgt
    17881 tgtatttatc tgcccaggta gagtaaaata gatcattatc taaaaggtaa ttgttcatac
    17941 tatataagtc atttgaaagt aaaaagatac tgtaaataaa aaatgtggtt aaataattct
    18001 cataatcagt gtaagcgtta tcaaaaatat agcatacttg ctttgtagat gaatgacacg
    18061 atatatgttt ggtatgttct gcaagacaga tgaatatgga ggtggagaca atgtcttcca
    18121 aagaactaac aatgtattta gagaaagaat tggatcaact gaaagaaaag gggttataca
    18181 atacgatcga catactagaa agtgaaaatg gtgcttgtat catcgtagac ggaaaaaaga
    18241 tgatcaatct agcatccaac aattatcttg gatttgccaa tcgcgaagaa ttgaaaaaag
    18301 catgtattga agcaaccgaa acttatggtg taggagcagg agcagtccga acgatcaatg
    18361 gttcgctgaa aatccatcag caattagaag aaaaaatcgc agaattcaaa gggacagaag
    18421 cagcaatcgc ctttcaatct ggattcaact gcaatatggg cgctatctct gcagtaatga
    18481 caaaagaaga tgctatctta tcggatgaat taaaccatgc atctattatt gacggttgcc
    18541 gcttatccgg agcaaaaatc atccgaatta aacatcaaga tatgaaagac cttgagaaaa
    18601 aagcaaagga agcgatagaa agtaagaaat ataaaaaaat catgtatatc acagatggtg
    18661 ttttctccat ggatggagac attgcacgat tgccggaaat tattccgatt gttgaaaaat
    18721 atggactgat cacctacgtc gatgatgcgc acggctcagg cgtaactgga aaaggtgcag
    18781 gaaccgtcaa gcattttgga ttaagcgaca aaatcgatat gcaaatgggc acgttatcga
    18841 aagctatagg tgttgttggg ggttatgtag ctggatcgaa aacactaatc gactggttga
    18901 aagctagaag tcggccattc ctattttcta cttcgctaac tccaggtgca gctgcagctg
    18961 cattggcttc gattactttg atgcaagaac atccggaact tgtcgaaaaa gtttgggaaa
    19021 atgctaatta ttttaaagaa gaattgaaaa aagtaggcta taacatcggt atgtctgaaa
    19081 cacctattac tccagtcatt ttgggagacg aaaaagtgac tcagactttc tctaagaagc
    19141 tgatcgaaca tgggatttat gctaaaccaa tcgtttatcc aactgtgcct cttgggacag
    19201 gcaggatcag aaatatgccg actgcggaac atacgaggga aatgcttgat gaagcagtag
    19261 cagtttatca aaaaatagga aaagaaatgg aaattattta actaaattta gttttttcgt
    19321 ctatttaaaa ttagagaaca tgttcacgca tatattgtta atcatctgaa gatagtagta
    19381 aaaaagatca gggaggatca tcatgaaaaa agtattggtg acaggatgtt taggacaaat
    19441 tggttcagag ttgatcacac ggctacgtca agaactaggc agtgaaaatg tcattgcgac
    19501 agatatacgc atgcctgaag tcattgagaa aaacgatatc tttgagatcc tagatgtaat
    19561 ggattacgaa aaaatgcgca gcatagcaga acaatatcaa gtggacacat tgattcattt
    19621 ggctgctctt ttgtcagctg ttgcagaagc aaaaccacaa ttagcctgga atttgaacgt
    19681 gactgggctg gtcaatgcat tggaagtcgc tagagaattc cagctgaaat tttttacgcc
    19741 aagttcaatc ggcgcgtttg gtccaaatac tccaaaagat cataccccac aagatactct
    19801 tcagcgtcct acaaccatgt atggggtaac gaaggtagct ggagaactac tatgtgatta
    19861 ttattatgtg aaatatggcg tagatactcg tggtgtccgt ttccctggat tgatttctta
    19921 tgcaaccttg ccaggaggcg gtacgacaga ctacgcagtc gatatttatt attcagcaat
    19981 caaagaagga aagtacacta gtttcattga tcaaggtaca tcaatggata tgatgtatat
    20041 gccagatgcg atcgatgcca ttataaaact catgaatgca gatcctgatc gattggttca
    20101 tcgaaatgcc ttcaatatat cggcgatgtc ttttgacccg gaagagatca aagcatctat
    20161 tcaaaaaatc ataccagatt ttgaaatgga ttatgatgtg gatcctgtac gtcagtcaat
    20221 agcagaatct tggccaaatt cactagatgt ttcttgtgcg caagaggaat ggggcttttc
    20281 gccgaaatac gacttagatg tgatgacaaa ggatatgttg gaaaaactga aagaaaaatt
    20341 ggtagagacg gtataaaaca agaaccacat ccaaaaagtt ttttatactt ttgggattgg
    20401 ctatggtatg aaaggggaca agggatggaa gaatatcaag ccacgccagt aaaagaagta
    20461 aaaatagaaa atgaattaaa acgtacgatg ggatttttta ctgctttatc gactgttatg
    20521 ggcacagtga tcggtgcagg ggtatttttt aaagccgcaa gcgtagcaga agttacagga
    20581 tccgccagtt tgcatatgtt ttcttggttt ctcggtggta tgatttctgt ctgtgcagga
    20641 ctaacaggag ctgaactggc agctgctatt ccagaaacag gagggatgat taaatatatc
    20701 gaacggattt acggaaatac agcagctttt ttattgggct gggcgcaagt cgtgatttat
    20761 tttccagcaa atgtagcagc cttgtcgatc attttcggta cacagttcgt caatctattt
    20821 ggtctttccc agtcaatgat tgttcccgta gctgttacag ctgctgtatc gatcctgttg
    20881 atcaattttt taggttctaa agctggaggg gcttttcagt cgattacgct tgtctgcaag
    20941 cttattcctt tgtttgtgat cgtcattttc ggtttgttcc gacaagaagg agtagacttc
    21001 caactgtttc cgatccaagc aggagaaaat ctttcatttt tctcagcctt gggagcgggc
    21061 ttgctagcaa cgatgttcgc atacgacggt tggatacacg taggaaatat ttcgggagaa
    21121 ttgaaaaagc cggcgaaaga tttgccaaaa gcaatttctt taggaatcat cggaatcatg
    21181 atcgtttatt tattagtaaa tgcggtgttc ttaagaaccg catctatcga cggagtcgca
    21241 ggaaacagca atgcggcaag tgacgttgca aaaatgattt ttggcggctt cggtggaaga
    21301 ctggtcacag ttggtatctt gatttctgtt tatggaacca tcaatggcta tacccttact
    21361 ggaatgcgtc tcccttatgt tatggcaaaa gaaaacaacc tgccttttag caagctcttt
    21421 gctaaacttc atgataaaac taaagttcct gtagccgcag gtatcttaga gctagtcatc
    21481 gctattggca tgatgatgat cggtggtttt gatacactaa cggatatgct gatctttgtc
    21541 atttggattt tttatacgat ggtctttgtt ggtgtcattc ttcttcgtaa aaaagaacct
    21601 gatttgtttc gaccatacaa agttccaatg tatccgttta ttccactagt agcaatcatt
    21661 ggagggactt ttatcgtatc aagcacgctt atcactcaga cctttttagc ttcgatgggg
    21721 atagtattga cacttgctgg tatcccgatt tatctctatt taaaacgtag atagctaaaa
    21781 cattcgaatt ttattagaac tgcacttctc tgccaaacaa aatattttat ttaaatgagg
    21841 agtacgtgga agaaatgtca aaagtactcg ttaattttga tcacgtaaat gtcgttttgt
    21901 ctacgatgaa atagcaaagt aaaagacaga aatcgggctc aaccgatttc tgtcttttac
    21961 tttgatttgc gtaactgaac tattcggcgt acgccgcgat aaatatttaa taaaaccaag
    22021 caaccagaaa tgaaaatggg aagccaaatc agatagacag ggtagccaag cactttagac
    22081 aaagcatgtg gagtcatttt atccatttca agaagtgctt gctgccaagt ctgaagattg
    22141 aagactagca tgaaacccga aataatcagg agaataaatc ctaaataatg aattgtagtt
    22201 ctttttctta cataagcgat cgtagcataa aggaacagta aaaaaatagg tacgccaaaa
    22261 acaaagtaca tattcagcat ctcgacaccc cctttgtgtt tatcttactt tactttgaaa
    22321 gaaatgagaa ataatagcct cgttacttgc ttgaaacagc aaaggactgc taagataaga
    22381 gagatacaaa gaatcaagga ggagtcatat ggcaagttat atcgaaccga ttcgttttgc
    22441 gctgattgtt tttccatttt tagccttagc gatctcatct gtttttttta tttatgaata
    22501 tcgaaagtat ggaacatttc tagtaacaag ggcagttatt ttgtattctt tcgtttttta
    22561 tttattgtgt gcgtattttt tggtgatctt accacttccg gcaaaggaga cggttgccca
    22621 aatgacaggg ccaaagatgg agctgcattt atttgcttca tggcaacatt tcagagacaa
    22681 cacggtattg gtcttgtctg aacctagtac ctacctgccg gcaatgaagc agagtgtatt
    22741 tttggaacca gcttttaatg ttttattact cataccattt ggcatttatt taagatatta
    22801 tttccggctt tcttttatga aaacgatgat tgctagtttc tgcctttctc tattttttga
    22861 attgacgcag ctaagtggac tttattttat ctacccaaga ccataccgct tatttgatgt
    22921 gaatgattta gtgacaaata cattgggtgg aatcattggt tttgtcgtta cgccgttatt
    22981 tacatttatg cttcctacaa gaagacgaat ggatgaactt tcttatgaaa aaggaaagac
    23041 tgtctcattg actcgacgct tggtcgctta tttgatcgac tggacatttt tatctcttat
    23101 taatttggta gcggcgattt tctttaaaat tgcgttcgat tcaggggcag ttacttctca
    23161 tatctggtgg cttttattag aagtaggggt atactttatg attgtaccgt ttataaccaa
    23221 tggatggaca cttggcaaaa aaattgtgcg tatccgtttg gtggaagaag accgcgaacg
    23281 aatttcttta aaagcattga tgatccgcta cgggtatctg tactttcttt tttatggttt
    23341 aagctggttg gggcagttgc ttcagcaaat catgaattcg acaacgaata cggtgttgca
    23401 gatgagtgca gtattcattt ctctcggtgt cttgttacta caaggtattt ttattttaaa
    23461 tatcttgcta tcatttttga gaaaaaaacg gattctgttt tatgaaaaag ctagtcacac
    23521 atatactgtt agcacgattg gccaaacaaa ggatgatgat tctcgaaatt agctgagcaa
    23581 aaacgctttg tctgtgttat aatgccaaag tcgtgataat ttaaagagaa gaggtgcacg
    23641 aatgaataat tttgatatat tccgcttcaa accgtttatc aatgaagcgt taaccgcaaa
    23701 gggcttcaaa cagcccacag aggtccagga acgtttgatt cctttaattg caaaaggaaa
    23761 aaatatcatt gggcaatctc aaactggtac aggaaaaaca catacgtttt tgttgccttt
    23821 gtttgaaaaa atcaatgcag aaaaagagga agtacaagtt gttattacag ctccaagtag
    23881 ggaactagct gcacagatct atgaagcagc tacacaaatc gctaaatttt ctacaccgga
    23941 gatccgtgtc tcgaattttg tcggcggtac agacaaacag cgtcaattaa atcgtctgca
    24001 tcaccaacag ccacaggttg tagtaggcac gcctggtaga atattagaca tgatgaatga
    24061 acaagcgctg aagattcaca ccgcatttgc atttgttgtc gatgaagcgg atatgacatt
    24121 ggatatgggt tttttaaatg aagtagacca aattgcaagt cgattaccag aaaagctgca
    24181 gtttttagtt ttttctgcca ctattccaga aaaacttcgt ccattcttac gaaaatacat
    24241 ggaaaatcca atcattgaag aaatcaaacc aaaagcagtt atttctgaag acattgaaaa
    24301 ttggctgatt tcaacaaaag gtaaagatga gaatcaaatg atttatcaac tgctgacaat
    24361 tggtcatcct tatttagcaa tcgtatttgc caatacaaaa cagcatgtgg atgaaattgc
    24421 cgattacttg aaaaaccaag gcttgaaagt agcaaagatt catggagaca tcaccccaag
    24481 agagagaaaa cgagtgatgc gacaagtaca gaatctggag tatcaatatg ttgtagcgac
    24541 tgatttagct gctagaggga tcgatatcga aggtgtctct catgttatta atgcagaaat
    24601 tccagaagat ctagacttct ttatccatcg tgtcggacgt actggacgta atggcttaaa
    24661 aggaacggcc attacgttat attcgccaaa tgatgagcaa gccattgatg cgatagaaaa
    24721 attgggcgtc tcatttgaac caaaagaaat caaaaacggg gaaatcgtca aaacatacga
    24781 tcgtaataga cgtgccaaac gagaaaaatc aaaagatgta ttagatccca cgctgattgg
    24841 tcttgtgaag aagaaaaaga aaaagatcaa gcctggttat aagaaaaaaa tcgattgggc
    24901 aattgcagaa aaaaataaaa aagaacgcaa aatcgaacgc agacagcaaa gccgtactgc
    24961 tcgtaaaaat aagaaaaatt cacataaata aaaaacaaga ggttgtgaca aaaggcgtgt
    25021 cacaatctct tgtttttaag ctagactttt tttcattccg atacaaagta cttcatcttc
    25081 aataaaaggt tctgtgcaag tatgataccc ttgtttttca taaaaatcta cagccgtcat
    25141 ttcagctact acataactag tctgacaacc ttctgaaacc gcccgttttt cagcagcttc
    25201 aagcaactga ccgcctaacc cttttcctcg gtattctttt gctacgcaaa atcgttccgg
    25261 cttgaagcaa gtaggtgttt tcttttcata acgcaaagta gctactggtc ttttttgatc
    25321 aagaaataga taataggttt gttgcgggat atcttttccg tcgaattcta gttctgcagg
    25381 aatcccttgt tctaatacaa aaacttgata tctcaaaaag cagctagctg cgcgtatcca
    25441 ttcatccgct ccaactattt ttttcatcat attcctcctt gtcatgaata gcagagatgt
    25501 attgattcct ttattcttat cgtaccaaaa atcgtggagt atgaccaaaa ggaaatgaaa
    25561 ataaaaattg agatattgac agtgatcaat agaatgcgta tactaaataa gtacgaaaag
    25621 gatatgattt gttttttaag actcacagaa aattctttct tggctgaaaa aagaatagtc
    25681 actaatcaaa ttgctccctt tttctatttt ggtagaaata ccgacgcagc tcagcgttat
    25741 cgagtttaag aggtaatgat tacacatcat tgcaatcaag gtggtaccgc gagttttcgt
    25801 ccttggctgt gatggtgttt ttttgtattt aatttataga aagcaggtaa tgacatgaaa
    25861 caattaagta gtgcacaagt tcgacaaatg tttcttgatt tcttccaatc taaaggacat
    25921 acgattgaac ctagtgcgtc tttgatcccc gtgaacgatc caaccttatt gtggatcaac
    25981 tctggtgttg caacattgaa aaaatatttt gatggttcag ttgtgccaga aaatccaaga
    26041 atcacaaatg cccaaaaatc tattcgaaca aatgatatcg aaaatgtcgg taaaacagca
    26101 cggcaccata cgatgtttga gatgctgggt aatttttcga ttggtgacta tttcaaaaat
    26161 gaagccatcc actgggcatg ggaatttttg actgatccaa aatggatggc attcgatcca
    26221 gaaaaacttt atgtaacagt ttatccaaaa gatacagaag caaaacgcat ctggcatgaa
    26281 gaagttggcc tgccactaga tcacattatt gatgtagaag acaacttctg ggatatcggt
    26341 gcaggcccat gtggtccgga tacggaaatt ttctatgacc gtggagaagc ttataacgat
    26401 ttagcagaag acgatcctga aaactatcca ggaggagaaa atgaacgtta tttagaaatc
    26461 tggaacttag tattttcaga attcaaccat aagcctgata acacatttga accacttcct
    26521 cataaaaaca ttgatacggg gatgggattg gaacgcatgg tctcaatcgt tcaagatgcg
    26581 tcaacgaact ttgagacaga cttgtttatg cctattatcc acgccgtaga aaagctaagc
    26641 aaacaagtaa aatatggtga aaacccagta acagatattt cttacaaagt aatcgcagat
    26701 catattcgcg ccctttcatt tgctattggc gacggtgcat tgccatcgaa tgaagggaga
    26761 gggtatgtct tacgccgttt gcttcgtcgt gctgtcatgc atgggaaaaa attaggtatt
    26821 gaacaagcat tcttatatcg tttagtccct gttgttgggg aaatcatggt aagttattat
    26881 cctgaagtac ttcaacaaaa agaatttatt gaaaaagtta tccgtacaga agaagaacgt
    26941 ttcaatgaaa caatcaatga aggtttggaa atcttaaatc aagtgatcca agaagtaaaa
    27001 aacgaaaacg ggacgactct agcaggaaaa gacatcttca aactatacga cacatatggc
    27061 ttcccggtag aattaacgga agaggtggca gaagatgaag gactgaaagt tgatcatgct
    27121 ggttttgaaa aagaaatgga tgcacaacgc gaacgtgctc gctctgcccg cagtactgaa
    27181 aaatcaatgg gggtacaatc tgctttattg acagatatca aagtagaaag taaattcatt
    27241 ggatacacag aacaagaatc agatagtcaa ttattggtca tcgttaaagg tgagtctctt
    27301 ttgaatgaag tcgcagaagg tgaagctcaa atgatctttg atcaaacacc tttctatgca
    27361 gaaatgggtg ggcaagtagc ggatcaagga tggatcatga atgaagtagg cgaaatcgta
    27421 gcagttgtta cagatgttca aaaagcgcct aatggtcaat ttatgcacac cgtagaagta
    27481 aaagcaccaa tgagtgaagg acaaacctat catctattga tcgatcaatc gatgcgcaat
    27541 cggatcatca aaaaccatac agcaacccat ctactgcaca aggcattgaa agaagtactt
    27601 ggcgagcatg caaatcaagc tggttcttta gtcgctcctg gacatttacg attcgacttt
    27661 acccactttg gtcaaatcac ttcagatgaa ttagctgaaa tggaaagaat cgtcaacgaa
    27721 aaaatctggg aagctatccc agttgaaaca attgaaacag atatcgatac agccaaaaac
    27781 atgggtgcaa tggctttgtt tggtgaaaaa tacggacatg acgttcgagt agtaaatatt
    27841 ggcggctggt caatcgaact gtgcggaggc acacatgtag caaatacaga aaacatcgga
    27901 atcttcaaaa tcgtttctga atcaggaatt ggtgcaggcg tgcgacgaat cgaggctgta
    27961 acaagtaaag aagcgtacga actacttgaa gaagaagaaa aacaactaaa acaagtagca
    28021 acaattgtta aatcacctca attgaaagaa gttgtttcaa aagcggaaca attgcaacag
    28081 caagtaagag agctacaaaa ggaaaatgaa caattagcta gcaagttagc gaatcaacaa
    28141 gctggtgatg tattcaaaga tccagaaacg atcaatggaa aaacaatcat tgcagcacaa
    28201 gtaaaagtga aagatatgaa tcagctgcgt caattagctg accaatggaa acaaaaaaat
    28261 atttcagata ttcttgtttt agcaaacgaa caagaaggaa aagtcagtct gctagcggca
    28321 atgacgaaag aatcaaatga agctggacta aaagctggcg acttgattaa agcaatcgca
    28381 ccgaaagtag gcggtggcgg tggtggacgt ccagacatgg cacaagctgg cgggaaaaat
    28441 cctgctggta ttccagatgc acttaaagct gcgaaagaat gggttgcgca acaatagggc
    28501 gtttacgttt gacagaaaaa caaaagttca aacattgaac gaaacatgat tatgggggcg
    28561 aagacaaaca agtcttgccc tcatactttt ttcaattgga tggcgcatct aatcaaatgg
    28621 tcggattcgg atggtcttta gttgacgaaa gtgacagaaa tttttacgat tagtataaat
    28681 aaaaaggggt gaaaaaatga atagtagaaa taagaccttt cgactagtct tacgtgcgat
    28741 cttgttagca atcatcatcg tacaagcgat ggttccttgg ctggggttta ttccattagg
    28801 attcattagt ttaacaatta tccatatcac agtaattatt gctgcagtag tattaggccc
    28861 aaaagatggc atggtgattg gcttgttttg gggaatcgct acaattgttc gtgcctatgc
    28921 catgccaaca acgccatttg atacattagt gttcacgaat ccgattatct cggtagttcc
    28981 cagagtactt gttggcttag tagcaggctt ggttttccac tggatttatc aaaaaaataa
    29041 atcaataacg atcagctcag cctttgcagg ggtactgggt tcattggtca atacagtctt
    29101 agtgttagga ttcatgggat tattttatac aggtgcaacg gcagatgcct atggtgtaga
    29161 ctctgctctt ttatttaaaa ccttagcagg ggttgcagca atcaatggga taccagaagc
    29221 aataggtgcg ggaatcataa caccattact agcaaaagcg ttatttgcag caacaccgtt
    29281 gaaaccagaa taatgaccat cttttaatag taataggacg cacgatagat agaaaaagga
    29341 agagaggaga ggaaaacaat cgtcctctcc atctttttac ctgagcaaca agcaaactag
    29401 aaggggtaaa aaacttggct aagaaacaag aagaaaaaaa gaaacaaata ccgcaacagc
    29461 ggacaaaaaa aggagtacta gcgggtatct taggattggt tacgaatctg atcttgtttg
    29521 tctctaagct aatgatcggg cttttttcag gaagtgtctc cattatggct gatgcagtga
    29581 acagtctatc agatactgct tcatccatat tgactttgat cggatttaaa attgctgcaa
    29641 agccagcaga tcaggaacat ccttatggac acgaacgctt cgaatatatc agtgggttat
    29701 ttgtttcgat catcattact tatgtaggat ttcaattttt agagtcttct gctgaaaaaa
    29761 tcatccatcc agaaaatgtc tctttaacac ccatcgtctt ttttgtccta atcatttcca
    29821 tctttttgaa atttgcacaa ggcaaaatgt atcaggttat tgcgcaatcc attgattctg
    29881 aaacattgag agctacagca acagatagtt ataatgatgt ctttactaca ctggctgtat
    29941 tgctttcagc agcaatcgaa tgggtaactg gatggcgtat tgatggatat ataggttttg
    30001 ctttagcatt atatatcctt tatagtggat tgacgatgct tcgaaatttt gtatacgaac
    30061 tattaggaag tcgcccaacg ccagcagaga ttgaagaaat ggaagagcat ttaaatgctt
    30121 gcgatgatat acttggctat catgatttat tggtgcataa ctatggaccc aataaacgct
    30181 ttgcatcagt ccacatcgaa gtagatgaat caatgaattt gaatgtggct cataagatta
    30241 ttgatctgat agaaaaagac tttaaaaagt ctttggatgt ggaacttgtc tgtcacttgg
    30301 atccaatgcc tgttgataac gaaaaatact ataatatctt gcggcagtta aagcaaattc
    30361 ttcttgaaac aggtacaggg cttaatattc atgattttag agtaattcat caaggaaaaa
    30421 cgctgcaatt tgatgtagtg gtccctgaga aattccagtt tgttgataca gaactagaaa
    30481 gaatcttacg tagtcaaata gaagaaaaaa tcggagatta ccaattggaa attacgtttg
    30541 atcacaatta tcttttatat taaatgagaa actataaaaa gactccttaa agtttttaat
    30601 ttaaagagtc tttttttctt ttaattgttt atggagagcc tgacgtgcaa gattatctgc
    30661 tcctttattt ttgttttcgg gcaaccattg caggatcaat aaagaaaatt cggatagaag
    30721 ttcttggatt ctttgaaggt agggaaggaa gagctcgttc ttcgttgcgt ttttgtcgac
    30781 cgtagaaact agtgttttgc tgtctgaaaa acagaaaatc gtttcattct gtaagtcgtt
    30841 tttctttaga aattctaaag tttttaaaaa gacttcgaac tctgcttgat ggtttgtccc
    30901 tatagaaagc gggatagata gctgttcttg tttaccttga tagacaatca agattccacc
    30961 tccactagga ccagggttgc cttttgtaga ggcatcaatg tatactttta acataaaaag
    31021 agattctcct ttttgttcaa actaatgtta tggtaagatt atatcattac tgaatcggtg
    31081 agaggttaga gaatgaaaaa aaatattcga tggcagcctg aactggcaca tacgatcatc
    31141 tattggtcat tgaccttgat cattctgttt tttagtctga ttctttcttt agaaaacaca
    31201 ggtccttatt ggaaaagcaa ttttgtttta ggtatttttc ttgtttttct atttctaggt
    31261 ttgcggcgtt cttttatttt aaaatcaaac tctgtgatta tacgttatgc tgctttttgg
    31321 aaagaccgag aaatatctgt cggacagatt cgatctttga cgccaactaa tactggggtg
    31381 caaatgatgt tgcaagatga gaaagcccca tctgttttca tgatgcgaaa aaaagatcaa
    31441 gtagtccttt ttgatcattt tgaaaaaaac ggacccaaat cagaatcaat tccttatcct
    31501 tatacgaaaa aaagaaaaag ctaattttaa aaatcagaac ctaaaaaaac gaatttttac
    31561 aggaaaagaa aagagtgaga cgtacggttc atcccgttta gcgtctcact ctatttgttt
    31621 atctattcat gcatactagt atcggtagtc tgaacgttgc ctttcttgac atgggcagct
    31681 tgtgttcctc gattcccttc gatgatctca aattcgacca cttgattttc ttccaaagtt
    31741 ttgaatccct cttcttcaat cgcagtaaaa tgtacaaaaa tttcttctgt gtcatcataa
    31801 ctgataaagc cgtatccttt tttgttgtca aaccatttta caattcctgt ggtcaaagca
    31861 ctcacgacct ttctaaagtc tgatcatagc cttcattata aagagtattt ataagtacgt
    31921 caaataaatt aaaaacctgt aattttatgt tttttttctg aataaaaaaa ggtataattg
    31981 gcatatctta acaaagaaga tattttgttg aataaagttc gtgaaaaggg gtaatcaaat
    32041 gaagaatgat ctgcaaattt cacaagaaac agagctagaa ccgattgttg aagtggctag
    32101 taaattagat cttaaggaag acgatttgga actgtatggg aaatataagg cgaaaatcaa
    32161 tttttctgga atcaatcgat caaaggataa cgctgagggg catttgatct tagttacttc
    32221 catcaatcca acaccagctg gcgaaggaaa gtcaacgatc actgtagggt taggagacgc
    32281 gctcaataaa atcaataaaa aatctgttat tgcattgcga gaaccctcac ttggtcctgt
    32341 gatgggaatc aaaggtggtg ctgcaggcgg aggatatgca caggtattgc ctatggaaga
    32401 tatcaacttg cattttactg gtgacatgca tgctatcaca acagctaata atgcattatc
    32461 tgctctttta gataatcata ttcatcaagg aaatgaattg aacatcgatg cccgccgagt
    32521 gatttggaaa cgggtagtcg atctcaatga tcgtgaactg cgtaaagtaa ttgttggctt
    32581 gggtgggcca atccaaggtg ttccaagaga agatgggttt gatatcacag ttgccagcga
    32641 aatcatggct atcttgtgtc ttgcggcaga ccttgaagat ttaaaggctc gtcttgcaag
    32701 gatcgttgtc ggctatacat ttgaccgtca gcctgtgact gctggagact tgaaagcaga
    32761 aggcgctctg gcattactgc tgaaagatgc aatcaagccg aatcttgtac aaacaattta
    32821 cggaacacca gcttttgtcc acgggggacc gtttgccaat atcgctcatg gctgcaatag
    32881 tattttagct accaagacag cattgagatt agcagattac gttgtcacag aggcaggatt
    32941 tggtgcagat cttggcggag agaaattttt agatatcaaa gtacctaact tgaaaaaagc
    33001 gccggatgct gtggtgattg tcgccactgt ccgtgcattg aaaatgcatg gcggaatgaa
    33061 aaaagatgaa cttaaaaatg agaatcttga tgctttgaaa gtaggttttg caaatttaaa
    33121 gcgccatata cgtaatatgg aacaatatca gctacctgtg atcgtcgcaa tcaatgaatt
    33181 cgtaacagat acagacaatg aactcacact gttggagcac ttgtgtgaaa atcaggggat
    33241 tcttgccaag agagcgagtg tatgggcaga tggagcagaa ggcggcgttg atttagcaaa
    33301 agctgtagta cgattgattg atagaaaaga agctaattac aagccattat accgcctaga
    33361 agaaacgata caagaaaaga cagaaacaat cgtcaagaaa atttacggag gaaatggtgt
    33421 tgttttcagc aaaaaagcgc aaaaacaaat agaagagttt acgaagaatg gatgggacaa
    33481 actgccgatt tgtatggcaa aaacacagta ttcattttct gatgatcctt cactgctagg
    33541 tgcaccggaa gatttcacca tcacgatccg cgaaatcatc ccaaaattag gggcaggatt
    33601 tttagtagcc ttgactggag atgttatgac gatgcctggt ttgcctaaaa aacctgctgc
    33661 attgaatatg gacgtgacaa atgacggaga agtgttggga ttgttttaag cttgattata
    33721 atctaccatc gaaggagctg tattttgact acagcttctt tttttagacg atagggaatt
    33781 aattttgttt acactaggat cattcttttt ctttattgta ttttgagcta tactaaagca
    33841 taataggagg gtatgatgaa aacgacattt ataagaaaaa ccggttttta cggtatgggt
    33901 agctcgattc agctaagaat caacaatgaa aaacgcagtg ttctcagtca tgaacaaaag
    33961 ataacgcttg atctggaagt gccctttacg atgcaggtca ccttttattg gctaaaaagc
    34021 cccgtttata cagtgaaaga agcaaagcct gcttatgtga tcacaatgaa ttacttgatt
    34081 cttcaaatct atccgttttt atttctagca actgggttgg gggccgtttt cctacaaaat
    34141 ttatttttca gtttttttgc agttattgtg ttgattgcgt tctttttcta tataaaaaac
    34201 cgggcatatt tgatcaagga ggaaaattat ggcaaactct gatctttttt taatcagttt
    34261 caatcgaatc gaaaaatgga tgagagacga gatgggaaat gcacgaaata tgggatttac
    34321 cgaacttgtc agacgcctag ctcaaagaaa acaattaatg atcagaaaat acgaagatga
    34381 tcttttacag cttgctcagt taaggaatgc gatcgtccat gatcggatcg cagtcgattt
    34441 tgtcattgca gagccaaatg attggacagt caagcgaatc cagctgatcg aacaagaact
    34501 gattcagcct gaaaccgtgc tgcctcgatt tgcaaaacgt gtaacaggtt ttgaacaaga
    34561 tcttccgtta cttgaactgt taaagatagt agcagaaaaa aggtattcac aatttccgtt
    34621 atacaataaa ggaaaatttg tagcattgat tactttacgt aatatcgggt tttggctagc
    34681 aaaagaaagc caaaaaggtc cggttgattt aacaaataaa cgagcgttgg atttgatcat
    34741 ccaaaacgga aagtacacaa attatcattt tgttccagct aaaacacata tttacgaggt
    34801 tgaagcgatg tttcgtgagc aagggacttt ggaagccgta cttatcacta aagatgggaa
    34861 tcccgatggt aatctgctcg ggatcgtacg acctcgtgat atttataaac aaatagaaaa
    34921 ggattgattt tttgttaatt atttattgga tattcagcgc tttgctagta ggagtggatc
    34981 agttggtcaa atggtggatc actgataatc tagcgcttgg agagacgaaa aatcttatac
    35041 caggtatttt gtcactgaat catattcgaa acactggagc cgcatggagt atgctagaag
    35101 ggaagatgtg gttctttact atagtgacac tcattgcagt cgttgtgatt ctcacattga
    35161 tgattaaaaa tcgtgaaaaa ggtaatcgct ggttcatgat tggattgagt ttgattctag
    35221 ctggtgcgat tggcaatttt atcgaccgta ttcgtcttgg ctatgtagta gatatgttcc
    35281 agacagattt tatgagcttt ccgattttta acgtagcaga tgtgacactt gtatgtggag
    35341 tgatttgtat gttaatttat attattttag atgaaaagga tcaaagaaaa aaatgacaga
    35401 acttaaaacg acgattcacc atgaatctgg taggatcgat aaagtattga ctaatttatt
    35461 caccgattac agtcgttcgc aaattcagct gtggctgaaa aatggagcgg tttctgtcaa
    35521 tggacagcca gtcaaagcaa attacaaagt aaaacaagga gacgagatta ctgtcttgat
    35581 ccctgaacca gaaacattag atgtggtccc agaaaatatc ccgttgacga tcgtttacga
    35641 agatgaagat gtagcggtga taaacaaacc tcaaggaatg gtcgttcatc cttcagcagg
    35701 tcatacgcat ggcacaatgg tcaatgcgct gatgtaccat atcaaggatc tttctacgat
    35761 caacggcgtt attcgtccag gtattgttca ccgaatcgac aaagatactt ccggactgct
    35821 aatggttgcc aaaaatgacc aggcacacga atcattagct cagcaattaa aagataagac
    35881 ctctttgcgc aaatatgttg ctttagtcca tggagagatt cctcacgaaa aaggaaccat
    35941 caatgcgcca atcggccgtt cgaaggaaaa ccggaaaatg caggcgataa gagaagatgg
    36001 gaagtcggct gtcacacatt ttactgtatt ggaacgtttc gaaggattta ctctggttga
    36061 attaaaacta gaaaccggaa gaacacacca gatccgtgtg catatgaagt atatcggtta
    36121 tccgttagct ggagatccag tatacggacc gaagaaaact ttgaaaggca atggtcagtt
    36181 tctccatgcg caaatgttag gttttacgca cccgactaca aacgaaaaga tgatctttga
    36241 agcacctctt ccagaagttt ttgaaaaaac tttagaaaaa ttaagaaaaa acattgcatt
    36301 ttaatttaga taacggtata gtaattaaca acgaaagcat aaaacttaat agaattcctt
    36361 taagtttagt cccgtgaggc taagaagggt gggagcaagg atacgtatgg tgcgccataa
    36421 gtagattgct cattgacata gtatagctat cagtgtatca gtatacgctc agtctaaaca
    36481 ttcctcctgt cctcttaggg caggagtttt tttatgcaaa aaagtagggg gaatgaaaat
    36541 gcaagcaaaa gaagtagtag atcaggtaac catgaagcga gcattgactc gaattactta
    36601 tgagattatc gagagaaacc atagtatcca agatatcgta ttggtaggta tcaaaacacg
    36661 aggaatctac attgcatcaa gaattgccga acgcctgaag cagctagaag atatcgatat
    36721 tcctgtagga gaactggata tcactttata ccgagatgac aaaaaagaaa atccagaaga
    36781 accggaactt cattcttctg acatcccagt ttccttagaa ggaaaagaag ttattttgat
    36841 tgatgatgtt ttgtatacag gacgaacgat tcgagcagca atggacgcag taatggattt
    36901 tggcagacca agaaaaattt cattagcagt cctggtcgat cgtgggcatc gagaattgcc
    36961 aatccgtgca gattatgttg ggaagaacat tccaacatct cgggcggaag agattttagt
    37021 agagatgcaa gagcttgatg gacaagatcg catcatgatt ttgaaagagg aagattagag
    37081 gagagagaaa catgtcggag aaaaaaggat ttcataatga tgacgtggtg ttagatatcc
    37141 acgatcgccc aaccccagga cattgggtag gattaagttt acaacacttg ttcacgatgt
    37201 tcggagcaac cgtgttagtc cccatcttag taggtatcga tccaggtatt gcgctagtca
    37261 gttcaggact aggtacattg gtgtacttat ttactactaa aggaaagata cctgcgtact
    37321 taggaagcag cttcgctttc attgcagcga tgcagctttt gatgaaaaca gatggttatc
    37381 cagcgattgc acaaggagca gtcacaactg gattagtcta tttgattgtt gcgctgatca
    37441 tcaaaaagat cggttcagca tggctggata aaatcttgcc tccaatcgtc gtcggtccag
    37501 tcatcatggt tatcgggttg ggacttgcgg caaatgcagc aacaaatgct atgtataatc
    37561 agggcacgta tgatttcaag tatattgctg tagcgctgat cacattagga ttaacaattt
    37621 tcttcaacat gtggctaaga ggttttctcg gattgattcc gattttacta ggtattatta
    37681 gcggatacat cgtagccttg ctgtttggaa tcgtggatac taagccaatc gtagacgctg
    37741 cctggtttgc tgttcctaac tttgagattc cctttgtaca atatgaacca aagctatatc
    37801 ttggagcaat cacaacaatg gcacccatcg cttttgtgac gatgacagag catatcggcc
    37861 atcttatggt attgaataaa ctaactaaac gtaatttctt tgaagaaccg ggtcttcata
    37921 agacgttgac aggtgatgga ttagcacaaa tcgtggcagc ctttgtcgga ggtcctcctg
    37981 taacaagtta cggtgaaaac attggtgttc tggctatcac acgtgtccat agcgtgtttg
    38041 tgatcggtgg agctgcagta tttgcagtca ttttaggatt cgtaggaaaa ttaagcgctt
    38101 tgattttaag tatccctgga ccagtcatct caggaatcag ttttatctta tttggagtaa
    38161 ttgccgcaag tggaatgaag attttaatcg agaacaaaat cgattttgat aaaaagaaaa
    38221 atctgttgat tgcttcagtt atcttagtag taggaattgg cggactcgtc tttgaagtag
    38281 gcacatttac attgtcggca atggcattag caactgtact tggcatcatt ttaaatctta
    38341 tcttaccaga aacttcacga agcgaagaaa actaaggagg aaacattatg atcatcaaat
    38401 cagaacgtat cagcttaaag catttattga ccgttgaggc acttactgat caagaagtta
    38461 tgggcttgat ccgcagaggg caggaattca agcaaggtgc taattggact ccgcaaaagc
    38521 gccagtactt tgctacaaac ctctttttcg aaaatagtac tcgcacacat aaaagcttcg
    38581 atgtagcgga gaaaaaacta gggctagaag tgattgaatt tgaagcaagc acaagctctg
    38641 tccaaaaagg ggaaacacta tacgatacag tactgacgat gtcagcatta ggcgtagacg
    38701 tagcggttat ccgtcatggt gatgagaact attatgacga actgatacaa agtaaaacaa
    38761 tccagtgttc gattatcaat ggaggagacg gaagcggaca gcatccaacc caatgcttgc
    38821 ttgatttgat gaccatttat gaagaatttg gtcattttga aggtctgaaa gttgccatcg
    38881 taggtgatat cactcactct cgcgtagcta aatccaatat gcagatgtta aaacgtctag
    38941 gcgcacaagt attcttctca ggtcctagag aatggtacga cgatgaattc gaagtgtacg
    39001 gacattatat gccattggat gaactactag atcaagttga tgtcatgatg atgcttcggg
    39061 tgcaacacga acgccatgat ggaaaagaaa gtttctcaaa agaaggctac catcaggaac
    39121 atgggttaac agttgagcgt gcgaagaagt tgcaaaaaca tgcaatcatt atgcacccag
    39181 caccagttaa ccgtgatgta gaattagctg attcattagt ggaaggatta cagtcgcgga
    39241 tcgttcagca aatgagcaat ggagtattcg tcagaatggc aatcttagaa gcagtgctaa
    39301 atggaaaagc gtaattatag gaggatgaaa aaatgaaaac actcattaaa aacggtcaga
    39361 tcaatacacg aaaaaatatg acgacaccag cagaaatttg gatagaagat gggcgaatca
    39421 aagcaatcgg tacaggattc tcagaagccg aatttgatga agtgtttgat gcaaagggac
    39481 aattgatcac acctggttta gtggacgtcc atgttcattt gagagagcca ggatttacat
    39541 acaaggagac gatcgaagct gggactcgct cagctgctcg tggcggtttc actactgtat
    39601 gcgcgatgcc gaatttaaat cctgtacctg atacggcaga aaagctgaga caagtatatg
    39661 acatcattag aaaagatgca gttgtgaagg tattgcagta tgcgccaatc acagaaaatc
    39721 tgcgaagtga gaaattagtt gatcaagaag cactgattga agaaggtgct tttgctttca
    39781 caaatgacgg tgtaggtgtc caaacagctg ggacgatgta tctggctatg aaagaagctg
    39841 caaaaaacaa taaagcccta gtagcccata cagaagatga atctttatta tttggcggcg
    39901 ttatgcacgc tggcaagaag gcagaagaac ttggtcttcc cgggatctta agcgtaaccg
    39961 agtcttctca aattgctaga gatctgttgc tagctgaagc aactggtgtt cattaccatg
    40021 tttgccatgt ttctactaaa gaaagtgtcc gtgtaatcag agacgcaaaa aaagcaggaa
    40081 tccacgtaac agcagaagtt tcaccccatc atcttatttt gatcgacgaa gatattcccg
    40141 aagatttcgg cttttggaaa atgaacccgc cgctaagagg acgggaggac agagaagctt
    40201 tgatcgaagg cttgcttgac ggtacgatcg attgcatcgc tacagaccat gccccccatg
    40261 gattagaaga aaaaagccaa agtttcatga aatcaccatt tggtatcgta ggaagcgaaa
    40321 cagcgtttca actgatctat actcattttg tagaaactgg tcgattcacg ttggaacaag
    40381 tgatcaattg gttggcagtt aaaccggctg agatttttgg tttgaatgca ggaacgctaa
    40441 cggttggtgc ccccgctgat gtagcagttt ttgatattac acaaacttgc acgatcgata
    40501 aagaagactt tttatcaaaa ggggagaata caccttttat cggctggaaa gtaaaaggcg
    40561 aaacacaaat gacttttgtc aacggaaaac ttgtgtggca gaagggagaa taaaagtatg
    40621 gaacgtttgc ttatattaga agatggaaca gttttcgaag gaaaggcttt tggtgcagat
    40681 gctagtgtct tcggagaaat cgtttttaca acaagtatga ctggttatca agaaactatc
    40741 actgatcaaa gttttaacgg tcaaatcatt acatttacct atcctatggt gggtaattat
    40801 ggggtaaata gagatgatta tgaatcgatt tctccaacat gcaaaggagt agtagtaaaa
    40861 gaacatgcac gtgtagcttc aaactggcga aaccaaatga cgctagattc atttttgaaa
    40921 caaaaaggaa ttcctggaat atcaggtatc gatacgcgcg cccttactcg caaaatccgt
    40981 gaagtcggaa cgatgaaagc tagtatagtt gatgctggag attccttcga acatgcattt
    41041 gatcagctga aagcaaccgt tctagcaacc aatcaagtgc agcaagtctc aactaacaaa
    41101 ccttatccaa gtccaggaac aggtcggaaa gtcattgtca ttgacttcgg cttgaaacat
    41161 agtattttac gagaattgag taaaagaaac tgtaacctca cagtacttcc ctataacaca
    41221 gatgccgaga cgattttagc gctatcacca gacggagtca tgctgacgaa tggacctggt
    41281 gatccaaaag atgtgccaga agcaatcgaa atgatcaaaa aaatccaagg aaaagtaccg
    41341 atttttggta tttgtctcgg tcaccagtta tttgctttag ccaacggtgc agatacctat
    41401 aaaatgaaat tcggacatcg aggattaaat cacccggtac gagaagtagc gaccggaaga
    41461 attgatttta cttctcaaaa tcatggctat gcagtcgatg aagccacgat tgatccagaa
    41521 aaactcatcg taactcatgt agaagtaaat gacggaacag tagaaggagt caaacacaga
    41581 gattatcccg ctttcagtgt gcagtttcat ccagatgctg cccctggtcc gcatgatgca
    41641 ctacatttat ttgatgaatt tatggaaatg atggacgcag gaaaggagca gtaaacagat
    41701 gccgaaacgt acagatatca agaaaattat ggtgattgga tctggtccga tcatcattgg
    41761 tcaagccgca gaatttgact acgcaggaac acaagcctgc cttgccttaa aagaagaagg
    41821 atatgaagtg gttttagtaa attctaatcc tgcgacgatc atgacggata aagaagtggc
    41881 agatcaagta tatatcgaac cgatcacact tgaatttgta tcaaggattc ttcgaaaaga
    41941 gcgtccagat gctctccttc caactttagg cggacagaca ggcctcaata tggcaatgga
    42001 gctggcagca tcaggtattt tagaagaatt gaacatagaa cttctaggaa caaagctttc
    42061 tgcgatcgat caagcagaag atcgtgatct attcaaacag ttgatgaaag aattgaatca
    42121 gccaatcccc gagtctgaaa tcgtcaatac agtagaagaa gctgtagatt ttgcaaataa
    42181 aatcggctat cctgtcatcg ttcgtccagc ctttacttta ggcggaactg gtggaggtat
    42241 gtgtgaaaat gaagaagaat tacgtatcgt atcagaaaat ggattaagtc tttcccccgc
    42301 tacccaatgt ttgattgaac gaagtattgc aggttttaaa gaaattgaat atgaagtgat
    42361 gagagattcg gctgataatg caatcgttgt ctgcaatatg gaaaattttg acccggtggg
    42421 tatccataca ggtgattcta tcgtatttgc ccctagtcaa acattgtctg accatgaata
    42481 tcaaatgctt cgtgatgctt ctctttcgat tattcgagca cttaagatcg agggaggctg
    42541 taatgtccaa ctcgctcttg atccacatag ttttaattat tatgtgattg aagtcaatcc
    42601 gcgtgtttct cgttcatctg cgttagcaag taaggcgact ggctatccga tcgctaagct
    42661 agctgctaaa atagcgcttg gattaacatt agacgaaatg aaaaatcctg ttacaggaac
    42721 gacttttgca gaatttgaac ctgcattaga ttatgttgta gctaaaattc ctcgctggcc
    42781 gtttgataag tttgaaaacg gagaacggct tttaggcacg cagatgaaag caactggtga
    42841 agtgatggct atcggtcgga atatcgaaga gtctttattg aaagctgtgc gttctttaga
    42901 aatcggtaca caccatatcg aacttcctga gttggcagac atcacggacg atgacctaat
    42961 ccaaaaagtg gttcgagcac aagatgatcg cctgttttat gtagctgaag ctattagaag
    43021 agggtacacc atagaagaat tagcggattt gacaaaaatc gatttattct ttttagataa
    43081 actgttgcat atcgtagaaa tcgaacagca gttgaaacag cagccgttgg atgacaagat
    43141 attgaaaaca gcgaaacaaa acggttttac agacaaaaaa gtggcggagc tgtgggaaat
    43201 aaacgaagaa gccgttcgcc agatgagaaa agaaaatgat ttgattcctg tatataagat
    43261 ggttgacact tgtgcagcgg aattcgaatc aaaaacacct tatttttata gttcctatga
    43321 atatgaaaat gaaagttcac gatcagaaaa gccctcaatt ctagtactgg gatctggtcc
    43381 tatccgtatc ggacaagggg ttgaatttga ctatgcgact gtgcattctg tcaaagcaat
    43441 ccagcaggca ggatacgaag caatcatcat gaacagcaat cctgaaacag tttcgactga
    43501 cttttctatt tcggataaat tatattttga gccattgacc ttcgaagatg tgatgaatgt
    43561 gatcgagcta gaacagccga taggagtaat cgttcaattt ggtggtcaga cagcaatcaa
    43621 cttagccgaa cctttgactg ctgctggtgt aaaaatctta gggacaacga tcgaagactt
    43681 ggatcgtgcg gaaaatcgtg atttattcga gcaagcgctg aaagagttgg atattcctca
    43741 acctttaggg gatactgcta caagtaaaga agaagccgtg gttatcgctt cgagaattgg
    43801 ttatccagtt cttgttcgtc caagctatgt attaggcgga cgtgcgatgg aaatcgttga
    43861 aaaccagcga gatttagaag attacatgga acatgctgtg aaagcatctc cagaacatcc
    43921 agtactagtg gaccgttact tgcttggtgg tgaatgcgaa gtggatgcga tttgtgacgg
    43981 tgaaacagta ctcatcccag ggatcatgga gcatattgag cgtgctggtg tccattcagg
    44041 ggattcaatg gctgtgtatc cacctcaaac attatctgaa tcgatcaaga aaacgatcga
    44101 agattacacg atccgcttag cattaggttt gaattgtgtc gggatgatga atatccagtt
    44161 tgtcatccat gaaaatgagg tttatgtgat cgaagtcaat cctcgagcaa gtcggactgt
    44221 tccattctta agtaaggtga caggaattcc aatggcacaa attgcgacga aagctatttt
    44281 gggagagaaa ctaaagacac ttggttatga aaacggactt tatccagaaa caaaagaagt
    44341 tcatgttaaa gcacctgtct tttcttttac gaaattgcag aaagttgata cttacttagg
    44401 gcctgaaatg aaatcgactg gagaagtaat gggatctgac agacaattag acaaagcttt
    44461 gtacaaggca ttcgaagctt ctggattaca cttgcctgaa tatggagccg tattatttac
    44521 gattgcggac gaaacaaaag aagaagcact agaattagct aaacgtttta cagaaattgg
    44581 ctatagtctg ataggaacga aacatacagc tgcttatttc gaaaaaaatg gtctggtcgt
    44641 tgaaacagta gcgaaaattt ctgaggaagc agcagaaaaa aatgtagtcg acttaataag
    44701 agatggtaaa acacaagtgg ttgtcaatac gattgataaa gatcggggaa atgcttctaa
    44761 agacggtttc atcatccgaa gagaagcagt tgaacatggt attccattgt ttacttcttt
    44821 agacacagca gatgcgatcg ttcgagtgat ggaatcccgt gctttttcaa ctaaccctat
    44881 ttaacggcga gcacatgtta agacaaaaag acgaaaaacc gttgcaggag gggacagatg
    44941 aaacaagaaa tgatggaaat cgttcagcaa aaacagctgg caccaaagat ttttgaaatg
    45001 acattgaaag gtgatctagt aaaagaaatg cagcaaccag gacagttctt gcatattctc
    45061 gtgccgcgtg cagacttgct attacgccga ccgattagct tgaatcatat cgaccacgaa
    45121 acaaatactt gccgtatcat ttatcgggtg gaaggtgagg ggactagagt cttctctact
    45181 ctttctgccg gagaccattt agatgtcatg ggtcctttag gcaatggttt tgatttgtcc
    45241 aatcttcata caggagacga agtatttata atcggtggag gaatcggtgt ccctcctcta
    45301 tatgagttat caaaacagtt agtcgaaaaa ggaattaagc caacgcattt tttaggtttt
    45361 gccacacatg aagtcgttta ttatgaaaaa gaatttaatc gtctaggaga aacaagaatc
    45421 gctactgacg atggaacata cggtatgcac ggaaatgtcg gtaatttatt gttaggaacg
    45481 aagacggaac ctacagctgt ttttgcatgt ggttcaaacg ggttattgaa gacagtggaa
    45541 cagctgtttt cttcgcatca aaatgtccag ttatcattag aatcccgcat ggcatgtggg
    45601 ataggggctt gctatgcttg cgtttgtcat acgccggaag atgaaacagg aacaaaaagt
    45661 gtgaaagttt gtgacgaagg accgattttt aaaataggag aggtaattat atgaatgaga
    45721 atcctttagc ggtcaaactt cctggcctag aattaaaaaa tcctatcatg ccggcaagcg
    45781 gctgtttcgg ttttggcaaa gaatacggaa aatactatga tttgaatcag cttggatcga
    45841 tcatggtcaa ggcaacgact ccaaatgccc gctttggcaa tcccacgcca agagtggcgg
    45901 aaacaccatc tggcatgttg aatgcaattg gtttgcaaaa tcctggtcta gacgttgtta
    45961 tgcataaact tcttccagaa ttagaggagt acaatgagtt acccatcatc gcaaatgttg
    46021 ctggtgcttg tgaagaagac tatgtagaag tttgtagcaa aattggcgaa gcaccaaatg
    46081 tgaaagcaat cgagctgaac atttcttgcc caaatgtcaa acatggcgga attgcttttg
    46141 gaacagattc tgatgttgct tttcaattaa cacaagcagt aaagaaagtt tcttctgttc
    46201 ctgtctatgt caaattgtcc ccaaatgtaa cagacatcgt tccaatcgct caggcaatcg
    46261 aagctggagg ggcagatggg tttacgatga tcaatactct tcttggtatg cggattgacc
    46321 ttaagacaag aaaaccgatt ttagccaatc aaacaggtgg actctccgga ccagcaatca
    46381 agcctgtcgc tattcgtttg attcaccaag tagcaagtat atccagcctt ccaatcattg
    46441 ggatgggcgg cgtgcagaca gtggatgatg ttcttgagat gtttatggct ggagcaagtg
    46501 ctgtagctat agggaccgcg aatttcaccg atccgtatat ctgtcctaaa ctaattaaag
    46561 agctccctgt gcgaatgagc gaactaggca tcgaatcgct ggaaagatta atcaaagaag
    46621 tgagagagga acgtgaacga tgaatcaact agaccaacga ccaattattg cattagattt
    46681 ttcaactagg caagaggtag aagactttct tcgatttttc ccgaaagaag aaaaattgtt
    46741 tgtgaaaatc ggaatggaat tgttctacca agaaggacct gaaatcgtac gttatctaaa
    46801 agaggctggt cataatgtat ttttagatct gaaacttcac gatattccga atacggtcga
    46861 aaaagcaatg cggggattgg caaagcttgg tgttgatctc acctgtgttc atgccgctgg
    46921 cggtattcgt atgatggagg cggcaatgcg aggattagaa gaggggactc cagaaggtgg
    46981 aaaacgtccg ctacttctag ctatcactca gcttacttct acaagtgaag aagaaatgca
    47041 tgccgatcaa ctaatcgaag tttctttaga aaaaagtgtg atccattatg ctagctgtgc
    47101 caaaaaagca ggattagatg gtgtggtttc ttctgcttgg gaagtagaag cgatcaaaga
    47161 aatggcagga gacgaatttg tttgcttaac tccgggtata agaccagaag gaacagtcgc
    47221 aggagatcag acaagagtag tcacaccttc tcaggcaaaa aagatcggct caacttttat
    47281 cgttataggt cgaccaatca ctcagtcaac taatccttat aaagcatatc aaacgattca
    47341 aacagaatgg agctaaccta aaatgaatgt tgaacaaagt atcgcaaaag acttattaga
    47401 aattgaagca gtatttttaa atccaagtaa tccttttaca tgggcaagtg gaatcaaaag
    47461 cccaatttac tgtgataacc gaatcacgat gagctatcca aaagtccgaa aagagatcgc
    47521 aaaaggatta gcaagcaaaa tcaaagaagc ttaccctgaa gtacaagtga ttgctggaac
    47581 tgcaacagca ggaatccctc atgccgcatg ggtagcggaa atactggatt tgccaatggt
    47641 ctatattcga agtaaggcga aagatcatgg gaaaggtaac caaatcgaag gacgtatatt
    47701 tgaaggtcag aagatggttg tgatcgagga tttgatttct acaggcggaa gtgtcttgga
    47761 agcagctgaa gcagcaaaaa gagaaggtgc ggatatcctt ggtgttgcgg ctatctttac
    47821 gtatgagttg ccaaaaggaa aagcaaattt cgaaaaagcc gaaatccctc ttatgacgtt
    47881 gaccaattat tctgtattga ttgaagcagc tttagaagat cgttatatcg atgagcaaga
    47941 attaactctg ctaaaagaat ggaaaaaaga tcctgaaaat tggcagattt aagcgcgctt
    48001 gatgaagata gagaaatccg atttgtgtct ccagaatcat caattggaat agcaaaaatc
    48061 caaaaactga ttgcgagaag catcttttca tcaggtatga aaagaaactt ctatcgtgat
    48121 cagtttttga tttttttaaa attcattttt ctaagactgg ttagcagaaa gaagagaata
    48181 atctcttctc ttttttctat tatatataga ttttctctat tttttggtaa gggagctgtt
    48241 tcaaaaatgg taaagagcgg aaatgttgcg aatatcagtt atttaatgat atgatattcg
    48301 gaaaaagaaa tctcaaaggg aggtaacttt tatggggttg aatgaattta ttttaggatt
    48361 gaacaattta gagacgatca caagggcacc tggatttttt aaatatacag agcatacagt
    48421 tgcagcacat tcgtatcgtg tagcatctat tgcgcaagtt ctaggagaca ttgaagaagc
    48481 gaatggacaa ccgatcgatt ggaaactgct ctatgaaaaa tcattgaatc atgactacac
    48541 agagcgtttt attggtgata tcaaaacgcc ggtgaaatac gcaagcaaag agttaagagg
    48601 gatgctgcaa acagttgaag aaaagatgac ggacgaattc atcaaccaag agattccagc
    48661 tgagttccaa gaagtttatc gccgtcggtt gttcgaagga aaagatgaaa ccgtagaagg
    48721 agaactatta gccatttcag ataaggttga ccttctttat gaatcttttg atgaaatcat
    48781 caaaaataac ccggaaaaag tttataagga gatgttcatt gaggcagtga ctacgattaa
    48841 aagctttgaa catcggcctt ctgttcagta tttctttaaa gagatatttc cggaactatt
    48901 gaaccaggaa ttttatggaa aagaggcgtt tttagaaacc gtctcaacga ttgtaaagaa
    48961 agaagaaaaa taaaaatgat atgagatgca gggggaagta tagttgaaaa agaatatgga
    49021 tgtcgaatgg ggctattctg gagacaaagg tccggaaaat tgggcgagtt tatgtgattg
    49081 gtttgcgaga ggtgcggaat ttccattgca gtcgcctatt catttgacca agggggaaga
    49141 aaccaaaata gaaggagaca cgctttcctt tcattatcaa aagcaacggt ttactgacaa
    49201 ggaattcaaa aacacgattc atttagtacc ttttgaccaa ctcagttatg tagagttcaa
    49261 gaaagaacgc tattatttga cagatattca tttccacttg ccttctgaac atatcatcaa
    49321 cggaaaacag gaagatattg aatttcattt tgttcatatg aacagcaaaa aagagaatct
    49381 agttgtgggt gtcatgtacc agctaatggt acaggaaggt tggctgtata atcaagaaaa
    49441 tggggaaagt tggaacttag aaaaacatga gcattgggta aatccggatg tgttctttcc
    49501 ggagcaaaaa agccatttcc attatattgg ctcattgaca actccgccta ccagcgggcc
    49561 aatccaatgg tttgtaatgg atcatgtagg aaaactgaac caagagtttc ttctcccctt
    49621 cgatggtcag tatgctcgac cgaacaaccg accaatccag cccttgaatg gaagagaaat
    49681 ttattatttc gaggagtttg agcaatgaat attcagcaga tgaaatacgt ggtcgctgta
    49741 gcaaacaatg gtagtttcag agaagcggca aagaaaatgt ttgtctccca gccaagtctt
    49801 tcagctggta tcaaagaatt agaaaccgag ttagggatca cgctgtttac tcgaacgaat
    49861 cgaggggcat atttaacaga agaagggcaa gacttcttaa atcgtgccga gaaaatattg
    49921 gtccagcttg atacattaga aactcactat ctaacgagtg aaaagagaga caatttttcg
    49981 atcgctgccc agcattatga tttcttaggt cctttgaccg cacagctact cgaggaattc
    50041 aaagacgaga tcaaacaatt tcggatcatg gagacgacaa cagcgagagt gatcgaagaa
    50101 gtcaaagagc agcacagtga gatcggcatt atttatatga atgagcataa tcaagctggt
    50161 atcagaagat atttacaaca aggagagcta agtggccgca gcattggaaa attcaaaacg
    50221 catatttttt taaggcaagg acatccatta gctgaacacg cagagatcac caaagaagaa
    50281 ttgttatctt atcctcaagt tcgttttaca caggatggaa ataatttccc gtatttttat
    50341 gaagacctta tcgagatccc tgatcaagaa acagtgattt atacgagtga tcgaggtact
    50401 ttgatgaata tcgttcttga aactgatgct tacgcttcag gatctggtat tgtgattgga
    50461 gggattcgcg agcacttgcg gttaatccct ttggcagaag gcgaactaaa tgaattttat
    50521 atcatttatt cggcgaaacg tccgctcagt gaaatcgctc aaagatttat caaagagctt
    50581 actcggttac tggaccagca gagctaagaa aagcagatga gaacgctgtt tattgaacac
    50641 cgttgatcaa aaaagagggg ttgtgacaga attttgtcac aactcctctt tttgtagcaa
    50701 acaatttata actaattgct gttgtgggaa agtacgcaaa tatgattcta gaaacactca
    50761 ttgattacct gttttgtttc atagctgtaa ttttttcttc ttctggtgtc acgataatcg
    50821 tcttttggtt ttcataaata acataaccag gcttggcgcc atttggtttt cggatatgct
    50881 ttacctgtac aagatcaacc ggcacttggg cagagtatcg gtattttgaa aaataggcag
    50941 ctaactcggc agcttctgta attgtttcat ctgagggctt gtcactttta atgatgacgt
    51001 gcgaaccggg tatatttttt gcatgaagcc aatagtcggt ttttttagct gtcttcattg
    51061 tcagctggtc attttgaaga ttgtttttac caacaagaat gagcgttccg tcagaagaaa
    51121 agtattgatc aggctgagat ggtttcttac gtttttgttt ttttaagttc ttctttttca
    51181 ggtatccttc tgaagtcaat tcttctttaa ttgcttcgat atccattggg ccggcaattt
    51241 ccagttgaga taagacagat tcaagatatt ggatttcatc tttggcttct tggatttgtt
    51301 cgccaatcag cttaacagcg ttcttcagct tttggtatcg atggaaatat ttctgtgcat
    51361 tttgatttgg tgtcaatgcc ggatctagtg ctattttgat tggccgatct tcttcataat
    51421 aattaggtaa aacaacttct tttgcgccgc gtggtacttg tgtcagaaaa gtagtaagta
    51481 gttcgccatt tctacggtaa ttctcagcgt tttcggattc tttgagtgtt tgttcacgtt
    51541 ttttcaattt atttttattt cgttttagtt cattttcgat ttttcgaatc agttctccgc
    51601 cttgttgttt tgcccgatct ttctctgctt tttcgtgata atagctatct aataattgac
    51661 tgagtgttgg ataagctgtt acagcagatg cctgttcgga caatacctga taagcaatcg
    51721 gtgtgaaaaa atctttattt tccgtttcgt aaaaagtagg aataggctga tggctgatgg
    51781 ctgagaaaaa ttcgtcccat acaaccatct tttcattagg acgttccagt aatcttttgg
    51841 tcagctcttg agcgatatcg aaaccgattc cctgaaactg acgttggatt gcttttggat
    51901 ctagttcagt ttgggaaagc cgctgaaaga ttttttcttt ctcggagcta aatgggttca
    51961 agacctcttg ttttggcgga gcaacatatt ctacaccagg aagcaaagaa cgataagtgt
    52021 tttgagagct tccaatgtgt ttgatcgcat ccagtatttt tccagtttct cgattaacta
    52081 agacgatcgt actatgacgt cccattaatt caacgatcaa tatgatattt tgtagatcac
    52141 ccaactcgtt tcgtttagca aagtggaaat ggatcactcg atcattttcg atttgttcga
    52201 tgtcttctaa aatagcgcca tccaaatatt ttcgaagcat catcacgaag ttaggcggat
    52261 tatcaggatt ctgataatcg atttgggtaa tctgtacacg agcataactt gggtgcgcag
    52321 ataataataa tcgatggttt ttgccgcgtg aacgaatcac cagcacgatt tcattttcat
    52381 aaggctgatg gattttcgag atccggccag atagtaacgt ttcacgcaat tcgttgacca
    52441 tcgcgtgggt aaatacacca tcaaaggaca tggttcattc ctcatttctt ttcataagta
    52501 tattcattat aacgaaaaaa agaagaaaaa ccaatttggc cgaatagtaa agcaaaagtc
    52561 tagaaaaatc ttggactaat tacataaaaa acagtgtttc tatcgttgaa atccaaattt
    52621 ttaggtataa taaaaaatag gagaaaaagt agaaaagaag gtgtttgtta tttgggaagt
    52681 agttaaatcg agagtacaag aaatgaatga acatttctca gtgtcattcg cagaaaatcc
    52741 aagtaagtat tttcgacgtc ttgtttttct gctcatctta tcattgctgt taacagttgc
    52801 aatgttttta ttgccggaaa aaatccgaga agagcaagta aatagtctcg tttattcgtc
    52861 cataccaacc agcaaattga aaccttttga ttacagcaaa gctgatcaga aaatcaaaca
    52921 aagccgggcg atatcggtga tgttcagcaa accgaacggg aaaacctatc aggacatgct
    52981 tgatgttttt aatgatcctg agcttatgga agaattgaat cgaccgatct tttattatcc
    53041 gatcgtttat gatgtacatg aactggaaca aaaatataac atacgaacgg atgaagtgac
    53101 atttattttc tttgatggag gaaaagaagc aaatcggata actgcgggta agggaggaat
    53161 aactgatttt gataaggagt tgatccctga actgaatcga cttccgttag ccaatatcaa
    53221 acaattagaa gaagaattgg atagaacggg agtaacttcc caaaattccg agattgctaa
    53281 ttgagacatt ggcgcgatga aacggtgggt ctatatagcg ctatttttaa ttatcagaac
    53341 aagtgatgtt tgatcttcaa aataacaaat aaaaaattaa aaaatatagt tgacgaaatt
    53401 caaaaattcg attagactta aatcaattct taaggaaagg gtgacctgta atgaacttat
    53461 caaaacgtat ttcagcagaa aacaaaaaaa attggcgcag ctggcgtaga taagttgtat
    53521 taatggtgag actttcccat agatacaact ttttagagtg catttctaaa gcttgtatct
    53581 atgctagaca tgaacattga ctgccgcata gattagaatc tatgcggttt tttgtgtaaa
    53641 gacaatgacc gctttggcca ttgtcttttt ttgtagactg gatacttgcc tgattataat
//
LOCUS       DACCDF010000010        52140 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCDF010000010 DACCDF010000000
VERSION     DACCDF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.010"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            330..1175
                     /locus_tag="IVK51_000560"
     CDS             330..1175
                     /locus_tag="IVK51_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6430179.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IVK51_000561"
     CDS             1456..2676
                     /locus_tag="IVK51_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP6430180.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IVK51_000562"
     CDS             2673..5393
                     /locus_tag="IVK51_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6430181.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IVK51_000563"
     CDS             5377..6321
                     /locus_tag="IVK51_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6430182.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IVK51_000564"
     CDS             complement(6390..7400)
                     /locus_tag="IVK51_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6430183.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IVK51_000565"
     CDS             complement(7580..7924)
                     /locus_tag="IVK51_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6430184.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IVK51_000566"
     CDS             complement(7917..8351)
                     /locus_tag="IVK51_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP6430185.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IVK51_000567"
     CDS             8489..9226
                     /locus_tag="IVK51_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430186.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IVK51_000568"
     CDS             9219..10430
                     /locus_tag="IVK51_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430187.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IVK51_000569"
     CDS             10637..11416
                     /locus_tag="IVK51_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP6430188.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IVK51_000570"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IVK51_000570"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP6430189.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK51_000571"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IVK51_000571"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP6430190.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK51_000572"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IVK51_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP6430191.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IVK51_000573"
     CDS             15018..16067
                     /locus_tag="IVK51_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP6430192.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IVK51_000574"
     CDS             16064..16342
                     /locus_tag="IVK51_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP6430193.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IVK51_000575"
     CDS             16317..16787
                     /locus_tag="IVK51_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP6430194.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IVK51_000576"
     CDS             16804..17106
                     /locus_tag="IVK51_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430195.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IVK51_000577"
     CDS             17123..17494
                     /locus_tag="IVK51_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP6430196.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IVK51_000578"
     CDS             17466..17825
                     /locus_tag="IVK51_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430197.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IVK51_000579"
     CDS             17997..19004
                     /locus_tag="IVK51_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6430198.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IVK51_000580"
     CDS             19029..20213
                     /locus_tag="IVK51_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP6430199.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IVK51_000581"
     CDS             complement(20650..22233)
                     /locus_tag="IVK51_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6430200.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IVK51_000582"
     CDS             complement(22811..22954)
                     /locus_tag="IVK51_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP6430201.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="IVK51_000583"
     CDS             complement(23176..23655)
                     /locus_tag="IVK51_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6430202.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="IVK51_000584"
     CDS             complement(23728..24012)
                     /locus_tag="IVK51_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430203.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="IVK51_000585"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVK51_000585"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="IVK51_000586"
     CDS             complement(24356..25642)
                     /locus_tag="IVK51_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP6430204.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="IVK51_000587"
     CDS             25780..26451
                     /locus_tag="IVK51_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP6430205.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="IVK51_000588"
     CDS             26417..26905
                     /locus_tag="IVK51_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP6430206.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="IVK51_000589"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="IVK51_000589"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP6430207.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="IVK51_000590"
     CDS             27866..28630
                     /locus_tag="IVK51_000590"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP6430208.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITKITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="IVK51_000591"
     CDS             28863..31076
                     /locus_tag="IVK51_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP6430209.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="IVK51_000592"
     CDS             31087..31533
                     /locus_tag="IVK51_000592"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP6430210.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="IVK51_000593"
     CDS             31704..32963
                     /locus_tag="IVK51_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP6430211.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="IVK51_000594"
     CDS             complement(33052..33999)
                     /locus_tag="IVK51_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6430212.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="IVK51_000595"
     CDS             34393..35685
                     /locus_tag="IVK51_000595"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP6430213.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="IVK51_000596"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="IVK51_000596"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP6430214.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="IVK51_000597"
     CDS             37578..38453
                     /locus_tag="IVK51_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6430215.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="IVK51_000598"
     CDS             38719..40602
                     /locus_tag="IVK51_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP6430216.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="IVK51_000599"
     CDS             complement(40677..40961)
                     /locus_tag="IVK51_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6430217.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="IVK51_000600"
     CDS             complement(40951..41685)
                     /locus_tag="IVK51_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP6430218.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="IVK51_000601"
     CDS             41814..42437
                     /locus_tag="IVK51_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP6430219.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="IVK51_000602"
     CDS             42629..43750
                     /locus_tag="IVK51_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP6430220.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="IVK51_000603"
     CDS             43757..46885
                     /locus_tag="IVK51_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP6430221.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="IVK51_000604"
     CDS             46904..50098
                     /locus_tag="IVK51_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6430222.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="IVK51_000605"
     CDS             complement(50244..50951)
                     /locus_tag="IVK51_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6430223.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51716..52114
                     /gene="spxA"
                     /locus_tag="IVK51_000606"
     CDS             51716..52114
                     /gene="spxA"
                     /locus_tag="IVK51_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP6430224.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17113 a         9068 c        10787 g        15172 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacgaagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgcaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttccgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttatttttt taatacagtc tcaatataac
    51241 tattcaaaca taaaataacg ttatttgatt tatttgttta tagtaaattt tatttagtaa
    51301 tcattgcttt aaaatactac gcttatataa cagagaaaat aagcgttata ataaagaaca
    51361 gtattattta aaatgacaga aaacattact attaaagaaa taaaaaacat aaaaaatctt
    51421 aagaaatcgg tttcatttag cttttgagat aaaataatag tacagtttta tacgctgttt
    51481 catatgttta gaaaatttga attagatttg aaaactctaa tttttaaatg ataaaaaaag
    51541 cgagaatgct ggttttactg tatattaacc ttagataact ttcatttttt taaaaaaatt
    51601 atgaaaaaaa ggtagctatt taaaagttga ttatgtataa tagaaacaga tgggagatta
    51661 ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa aaggagtggt gcacgttgtt
    51721 aacattatat acttccccaa gttgtacttc ctgcagaaag gctcgtgcgt ggttgcaaga
    51781 acatcagatt ccatttgttg aacgtaatat tttttcagaa ccattgaaca gttctgaatt
    51841 aaaagcgatt ttacaaatga cagaagacgg aacagaagaa atcatctcga cacgttccaa
    51901 agtattccaa aaactgaaca tggatttaga tgatctgccg ttacaagaat tattggaact
    51961 tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg attgatgata aacgtctgca
    52021 agttggtttt aatgaagatg aaattcgtcg gttcctaccg cgcgatgtac gacaattaga
    52081 attacgtcaa gcacaattga tggctggatt atagaaggat aatacgaaac ttggaaaaga
//
LOCUS       DACCDF010000011        51804 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCDF010000011 DACCDF010000000
VERSION     DACCDF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.011"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..100)
                     /locus_tag="IVK51_000607"
     CDS             complement(<1..100)
                     /locus_tag="IVK51_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP6430225.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVF"
     gene            complement(115..1362)
                     /locus_tag="IVK51_000608"
     CDS             complement(115..1362)
                     /locus_tag="IVK51_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6430226.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(1438..1584)
                     /locus_tag="IVK51_000609"
     CDS             complement(1438..1584)
                     /locus_tag="IVK51_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP6430227.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(1688..1999)
                     /locus_tag="IVK51_000610"
     CDS             complement(1688..1999)
                     /locus_tag="IVK51_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6430228.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(2001..2198)
                     /locus_tag="IVK51_000611"
     CDS             complement(2001..2198)
                     /locus_tag="IVK51_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP6430229.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(2592..4319)
                     /locus_tag="IVK51_000612"
     CDS             complement(2592..4319)
                     /locus_tag="IVK51_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6430230.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(4312..5505)
                     /locus_tag="IVK51_000613"
     CDS             complement(4312..5505)
                     /locus_tag="IVK51_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6430231.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(5692..6336)
                     /locus_tag="IVK51_000614"
     CDS             complement(5692..6336)
                     /locus_tag="IVK51_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6430232.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(6430..6564)
                     /locus_tag="IVK51_000615"
     CDS             complement(6430..6564)
                     /locus_tag="IVK51_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP6430233.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IVK51_000616"
     CDS             complement(6566..8698)
                     /gene="feoB"
                     /locus_tag="IVK51_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP6430234.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(8695..9168)
                     /locus_tag="IVK51_000617"
     CDS             complement(8695..9168)
                     /locus_tag="IVK51_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP6430235.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            9569..9793
                     /gene="nrdH"
                     /locus_tag="IVK51_000618"
     CDS             9569..9793
                     /gene="nrdH"
                     /locus_tag="IVK51_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP6430236.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9952..12111
                     /gene="nrdE"
                     /locus_tag="IVK51_000619"
     CDS             9952..12111
                     /gene="nrdE"
                     /locus_tag="IVK51_000619"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP6430237.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            12161..13126
                     /gene="nrdF"
                     /locus_tag="IVK51_000620"
     CDS             12161..13126
                     /gene="nrdF"
                     /locus_tag="IVK51_000620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP6430238.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(13215..14354)
                     /locus_tag="IVK51_000621"
     CDS             complement(13215..14354)
                     /locus_tag="IVK51_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6430239.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(14663..15376)
                     /locus_tag="IVK51_000622"
     CDS             complement(14663..15376)
                     /locus_tag="IVK51_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP6430240.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IVK51_000623"
     CDS             complement(15630..16331)
                     /gene="nagB"
                     /locus_tag="IVK51_000623"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP6430241.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            16565..18097
                     /locus_tag="IVK51_000624"
     CDS             16565..18097
                     /locus_tag="IVK51_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6430242.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQINWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(18288..19490)
                     /locus_tag="IVK51_000625"
     CDS             complement(18288..19490)
                     /locus_tag="IVK51_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6430243.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(19572..20261)
                     /locus_tag="IVK51_000626"
     CDS             complement(19572..20261)
                     /locus_tag="IVK51_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6430244.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IVK51_000627"
     CDS             complement(20367..20735)
                     /gene="rplL"
                     /locus_tag="IVK51_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP6430245.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20788..21291)
                     /locus_tag="IVK51_000628"
     CDS             complement(20788..21291)
                     /locus_tag="IVK51_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP6430246.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IVK51_000629"
     CDS             complement(21701..22390)
                     /gene="rplA"
                     /locus_tag="IVK51_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP6430247.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IVK51_000630"
     CDS             complement(22494..22916)
                     /gene="rplK"
                     /locus_tag="IVK51_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP6430248.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            23259..23927
                     /gene="sdaAB"
                     /locus_tag="IVK51_000631"
     CDS             23259..23927
                     /gene="sdaAB"
                     /locus_tag="IVK51_000631"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP6430249.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23940..24812
                     /gene="sdaAA"
                     /locus_tag="IVK51_000632"
     CDS             23940..24812
                     /gene="sdaAA"
                     /locus_tag="IVK51_000632"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP6430250.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24903..25922)
                     /locus_tag="IVK51_000633"
     CDS             complement(24903..25922)
                     /locus_tag="IVK51_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP6430251.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(26095..26982)
                     /locus_tag="IVK51_000634"
     CDS             complement(26095..26982)
                     /locus_tag="IVK51_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6430252.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IVK51_000635"
     CDS             complement(27059..27604)
                     /gene="nusG"
                     /locus_tag="IVK51_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP6430253.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IVK51_000636"
     CDS             complement(27705..27875)
                     /gene="secE"
                     /locus_tag="IVK51_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP6430254.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IVK51_000637"
     CDS             complement(27899..28051)
                     /gene="rpmG"
                     /locus_tag="IVK51_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6430255.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            28213..28857
                     /locus_tag="IVK51_000638"
     CDS             28213..28857
                     /locus_tag="IVK51_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6430256.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28913..29416)
                     /locus_tag="IVK51_000639"
     CDS             complement(28913..29416)
                     /locus_tag="IVK51_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6430257.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(29429..29475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            30013..30978
                     /locus_tag="IVK51_000640"
     CDS             30013..30978
                     /locus_tag="IVK51_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP6430258.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(31077..32249)
                     /locus_tag="IVK51_000641"
     CDS             complement(31077..32249)
                     /locus_tag="IVK51_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430259.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(32236..33381)
                     /locus_tag="IVK51_000642"
     CDS             complement(32236..33381)
                     /locus_tag="IVK51_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430260.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(33395..34319)
                     /locus_tag="IVK51_000643"
                     /pseudo
     CDS             complement(33395..34319)
                     /locus_tag="IVK51_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            34436..35515
                     /locus_tag="IVK51_000644"
     CDS             34436..35515
                     /locus_tag="IVK51_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP6430261.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            35547..36140
                     /locus_tag="IVK51_000645"
     CDS             35547..36140
                     /locus_tag="IVK51_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430262.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(36250..36477)
                     /locus_tag="IVK51_000646"
     CDS             complement(36250..36477)
                     /locus_tag="IVK51_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6430263.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(36580..39168)
                     /locus_tag="IVK51_000647"
     CDS             complement(36580..39168)
                     /locus_tag="IVK51_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP6430264.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(39188..39847)
                     /locus_tag="IVK51_000648"
     CDS             complement(39188..39847)
                     /locus_tag="IVK51_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6430265.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IVK51_000649"
     CDS             complement(39896..40399)
                     /gene="trmL"
                     /locus_tag="IVK51_000649"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP6430266.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(40620..41486)
                     /locus_tag="IVK51_000650"
     CDS             complement(40620..41486)
                     /locus_tag="IVK51_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6430267.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            41595..42236
                     /locus_tag="IVK51_000651"
     CDS             41595..42236
                     /locus_tag="IVK51_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP6430268.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IVK51_000652"
     CDS             complement(42298..43659)
                     /gene="mgtE"
                     /locus_tag="IVK51_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP6430269.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(43677..44576)
                     /locus_tag="IVK51_000653"
     CDS             complement(43677..44576)
                     /locus_tag="IVK51_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6430270.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(44578..45375)
                     /locus_tag="IVK51_000654"
     CDS             complement(44578..45375)
                     /locus_tag="IVK51_000654"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP6430271.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(45353..46057)
                     /locus_tag="IVK51_000655"
     CDS             complement(45353..46057)
                     /locus_tag="IVK51_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP6430272.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            46188..46763
                     /locus_tag="IVK51_000656"
     CDS             46188..46763
                     /locus_tag="IVK51_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP6430273.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            46881..47558
                     /locus_tag="IVK51_000657"
     CDS             46881..47558
                     /locus_tag="IVK51_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6430274.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IVK51_000658"
     CDS             complement(47637..49445)
                     /gene="pepF"
                     /locus_tag="IVK51_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP6430275.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(49621..50619)
                     /locus_tag="IVK51_000659"
     CDS             complement(49621..50619)
                     /locus_tag="IVK51_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP6430276.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(50845..51498)
                     /locus_tag="IVK51_000660"
     CDS             complement(50845..51498)
                     /locus_tag="IVK51_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP6430277.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15619 a        10777 c         8970 g        16438 t
ORIGIN      
        1 taaatacttt atcgtgaaaa agaatgaaat tatcaataat tcgttctaac tgatttaatt
       61 gtataatttg atcttcacat aatattattg gataactcat aaatcctcct tccctcaaaa
      121 caacaaaata acttgagtag taaattgata cggatcgttt tgatagttca caaacatatt
      181 gggattcttt ttattgattt cctctatact agataaacct aacccttgat gtcccttttt
      241 tgttgttatt ccttttttca ttagagtatc tatagagagt agtatctctc tacaactatt
      301 cgatatcgaa atttctatct gtttatcatc tcggtaaata gctagagaca gttctctatc
      361 ttgactctct atagatgctt caattgcatt atcgagaata attccaagca ccctaataca
      421 gtcaaatatt ggaacaggaa cttcaataat agtacttgga cattcgaatt tacaacaaat
      481 cttattgtta tttgcttggt atagcttcgc tatcaacaaa cttttcaaat attcattttt
      541 tatatttttt aaataacgat attcaaactt attggttaga taagaattag aatagatctc
      601 taattctttt atttcttttt ccaatatcga atcatggttt tgattagcga tttcttttaa
      661 actcagcaat aaatttttat agtcatgtct aaatctggca aatttttctt gatctttttc
      721 cagtgaatca gtatattttt ttagataaac caattctttt cttttcaatt gctgttcata
      781 ttgttctcgt tgcctaatag ttattctaat aaataaaaat agaacaacaa acatttgaat
      841 gattaaaaat attaagacac caaacacaaa atgatcaaat acttgatatt gatgagcaac
      901 ataagaaaca aataaaccaa ttgtaagtaa ataaatcatg aatatagcag taagtttaga
      961 actggactgt tcaacaatcc tatttatagc taattttcta tataaccaaa taaacatcag
     1021 caaaaacaaa gaatcaagca ttatctggaa tactacataa ccataccctt ttatcccttc
     1081 aaatgaaaac atatacgtca taaccatgga agtaataata gatctgaaat catcaataag
     1141 catacataat agtaatttat ttaaaagcac tatattagtt ttcttctttt cctttaatat
     1201 aaaataactt ccgattatag gtatagctgc ggaaacatca gtaaataatt caacaaaaaa
     1261 agtgagtggt atcaataaac atacaaaaag atattttctt ttgttaagat cagtattagt
     1321 catataaaat aagagaaaaa agaaaaagtt tcccacgaac attcttaata gataagcttt
     1381 tgtatcgata gtaatcaaga gatcccctcc tgattttagg atatcataaa aaaaatttta
     1441 gttacacttt ttaaataatg aaaaaacaca ttccactaaa tttgatgccg gttttccttg
     1501 aggttttgtt ccagcacctc ctttaatttt tttaagctct tgatctgaac attgtttagc
     1561 atttagtcta tttttttctt ccattacgat tccccctaca aactataaat acttttatgt
     1621 cactgattca atatacctat ttttctaatc aaaaaatttt gagacatatt taatattata
     1681 agtatcatta aaattgagat ttatctccat aatctgctcg atataaacca tttattccag
     1741 cagttcttcc aatttcactc aaatttctta ttaattcctc ttgatattca gtgaatctta
     1801 ttttattagt ataagcagta aaatatagat aattcaccaa tctatttatt aaaggaccgg
     1861 gatctaattc atatttttgt ttttctaatt ttaaatatac cttttgtaaa acctcaagaa
     1921 tatcagaata tttaggatct ttacttatag atatatcatt atataattca tgaacaattt
     1981 gcttagcatt ttttttcatt ttagcacttc cctggaattg ctccacctaa aaaaccacct
     2041 atagacattc ctgcaataca agtagttgcc ttggcccaat cgaccgtaca tttattttta
     2101 gtgcaataca ctccatttcc ataatatttt ccactatgag tggtaccccc atagataagt
     2161 tgtgtctctt taatagacaa aatttttaaa tgtttcataa tctaatcacc cctaaattaa
     2221 tgtttttaga ctattattct aacattcatt aaatttcaaa aaacaattcc ttcttgaaac
     2281 gtagcaaaat aattcctgaa taagaattta atcactttta agtatataaa ctatgtatat
     2341 tctttaatat tttttgagaa tgcacttcgt acataataaa ctttaatcac attagtcttt
     2401 catacctacg atcgatctct ttgactcgca cccataaatt cataatagtt tgtcggcgtc
     2461 tttggacgtt tcgaagatga gctacgcatt ctcttttttt atagacaaaa aagatagaga
     2521 cgaacatgga gcttccaaat ttggatattt gatccaattt tggaagctcc atttatttcg
     2581 tgtctgtctt tttatttttt ccagctttcg actaattctt ggttctcttt gatccagtct
     2641 tttgctgctt gctgtggatc ttttccattg ttgatttcca acataacttt ttccatatct
     2701 ttttctgacc aatggaaatt gtctaatact ttgtaagctt ctggctggtc ttctttcaat
     2761 ccttttcgaa ccatcgtatg gatctgctct tcgcttccca ttccgttttc aggatcttct
     2821 aagtatttca gatcatattt ggcaaacatc caatgaggtg tccatcctgt aactacaatt
     2881 ggttcgtgtt ttttgatcgc ttgacctagt gcaacggtca ttgctcctga agaagatgtc
     2941 tctactttcc agtctttcaa gttgtcatat ttttggatcg tgttttcagc agccgtaact
     3001 acaccagctc ctggctcgat tcctatgatt ttcttacctg cctgatcggt taactcatcg
     3061 atcgaattta catccatgta ggctggcact gctaaaccaa cttttgctcc tgtcaaattg
     3121 gcacctaggt cttcgacttg atctttatat tgtgcatatt gtgaaccatg agttttaggt
     3181 agccaagctg aaaccatggc atctgactcg ccatttgaaa cagatttcca catgattgaa
     3241 ttatctaatg gcgtcatcgt tacatcatag cccatttgtt tcaatacttc accgactaca
     3301 tttgttgaag caacttctgt atcccattca acataagata agttgatacg ttttgtttct
     3361 ttggcagatg aaaaactccc tataccaatc gcacctaaga tcactactac tacagcacca
     3421 atgatcagtc cttgacgttt tttacttttc tgtgttgttg ctcctgcagc ttttttattg
     3481 tttggctggt tcaaatgctg tgtaaaacgg tcaatgatga ttgctaagat aactaatgca
     3541 accccgttga caaatccatt cccgacttgt gctctttgca atgctgataa tacgccgcgt
     3601 cctaagccgg gtgcaccgat catagaggca ataacgacca ttgaaagtga cagcattgtt
     3661 gtttgattga tccctgccat gatcgtactt ttagctaaag gcaattctag tttgaatagt
     3721 ttttgacgac ttgtgctccc gaatgaatca gaagcttcta ctaattcttt aggaacttgt
     3781 cgaatcccta aattcgtaaa acgaacggtt ggtggcagag caaagatgac tgaggcaaat
     3841 acccctggaa ccatcccgat accgaaaaat gcaactgctg gaatcaaata aacgaatcct
     3901 ggcattgtct gcataaagtc aaggatcggt ttgataatac tttgggcttt actgcttttt
     3961 gccatcaaga tccctaatgg taccccaatg atgatcgaga tcacactaga taaaagaaca
     4021 agtgtgacag tattcatcaa atcattccat agaccttggt tataaataaa ccatagaccg
     4081 attaatgtga acaacgtaag tcctggacgt ttcttagaaa taaagaaagc tgctaccgtc
     4141 aataacaaga taaacaaagg agctggaatc actaacagta aatttgtaat ccccgacatc
     4201 actgcctgtc caatcgtttg taaaaatgaa aataggccag aaaaagttgt cgtaaaccat
     4261 tctgtgatat tttctaccca gtttgccact ggtaattgat agttcaatag attattcatg
     4321 ttcactcacc tcgcttgttt ctgctaatgc ttcaatcaca ctacctcgga tgacgacacc
     4381 ttttaatttt ccatcctcta caactgcaag tggagtcggt gagtcataaa taatattgaa
     4441 aatatcactt accaccatgt ctttcccaat caccgtcaca ttttcatcta ggaagtcgat
     4501 caatggacgt ttttgcttcc tagcttctaa cgcttgatct gcagtgatga tccctttcag
     4561 gtgtctcttt ctgtcaacag ccatcagcat gctgacttct tcattacgca tccttgtcaa
     4621 tgcaacgttt ggtccatcag attcgatatt cgtcgtcaat gcaggtacca tgatgttttg
     4681 agccgtaagc actttggaac gatcgacttc ttccacaaat tcacgtacat aatcatttgc
     4741 tggattggtc aatatttctt cacctgttcc gatctgcatg atttcgccgt ctttcatcaa
     4801 ggcgattctg tccccaatcc gtaaagcttc attcaaatca tgggtaatga aaataatcgt
     4861 tttttgaaca ttggcttgca ggtccagtaa ttcgtcttgc atttctcgcc ggatcagcgg
     4921 atcaagagcc gagaacgctt cgtccatcag caagatctca ggatcatttg ctaaagctct
     4981 ggctaggcca acccgttgtt gcatcccacc agataactga ctaggatatt ggtctttaaa
     5041 tgaaagcaaa ctagaatttt ctaatgcttt ttctgctttt tcttgtcgtt cttctttagg
     5101 tacaccacgg acttctaacc cgtattctgt attttccaag atcgttcgat gtggaaacag
     5161 accgaagttt tggaaaacca tattgatttt atgacgacgg acttctcgca attcttcttt
     5221 gcttaacttc gatacatctt ctccatcaat atagatactg cccgaagttg gctcaatcag
     5281 acgattcaat agacggatca atgtggattt cccactcccg gaaagcccca tgatcacgaa
     5341 gatttctcct tcttttacgt caaaattcac gtcatacaca ccaaccgttg caccggtttt
     5401 ctttaaaatc tccgttttcg gctggtgatc attcatcata tctaaagcgg cttgtgtctt
     5461 tttaccaaat atctttgtta aatgttttac ttctactttt accaaacaaa ttctcctttt
     5521 ttatgtaaat tcatttttct ttctgaaaaa agtgaccaca ctatgttaac atcatgtagt
     5581 cactttgtca aaaatgaaag aaatttgctt gtccaaactg aaaaaaactt ggctaaatct
     5641 ttgtaaataa aaggtttagc caagttaata tttttttaat atttcacaca atcattctgc
     5701 aagctgatgc tccgggtaaa aaaagtttgt catacgctgt agtgtatatt catttccaat
     5761 aaaatgaacg atgtcccctg ccgtaaattt agcatagggg ccaggtgaaa caagcagttc
     5821 ttcattatga gaaattgcga taactgtggc gccagtcgcc tgccagatat tcaactcgct
     5881 aattgaacgg tccagatgca ttgctttttc tgataaagat aattcaaacg gaagtaaagg
     5941 attcatctta tgtattcgct tcgtttgatt gaccagctga tccaataatt gagaaaaatg
     6001 attcagctca tcttgttgct tctccacact tgccatgatt tctgcttttg tatcttggat
     6061 ggattgtaca tcttgatatt gcaataggaa atcttttgct ttttccttcg aagcaacaaa
     6121 tgccccactt ccatgccgta cttccatgat tccaagatct accagcacat tgatagcctt
     6181 gcgggtagtc tcaggagaag cattgtacgt actggcaagt gttgagcggg catggatctt
     6241 ctcacccact ttatatcggt tttcaacgat acgttcggct aaatcaacag caatctgctg
     6301 gtagcgcggg cggacgattt ttttctttcc agacattttc tttcctcttt ctttttttac
     6361 tttccctcat cttactatgc tctagcaaaa taagcaaaaa aggggagaat agcctcttgc
     6421 aacgactgat catcgcttga tctgttcctt ttttactggg caagctgttt gacagtcatc
     6481 acaattctta ccggatttta aacgagtata gacaattgcg cctgcacttc caaagattaa
     6541 cacacttaat aagaatgtcg ccatcctatt cctctccttc taatgacaat atagtataaa
     6601 actggttctt ggattttggc tgacggaaca aaacataaac gagtcctatc atcagtaaaa
     6661 tagcaagata cgtccctaac gctacctgtc caccctccaa taagtgtccc aactgataaa
     6721 tcacaaaact aactaaataa gctaaaccgc attgatagcc tatggcgatc caagtccatt
     6781 tcttggcatt catttcccga tggatagccc caattgccgc aaaacaaggc gcacaaagta
     6841 aattgaaggc taacaatgaa taagctgctg cgggagtata tgtgtgttgt agcacttgcc
     6901 agatttctct gccattttcg gatacttccg catgaccaaa caaaatacca aatgtgccaa
     6961 taatattttc tttagcgacc aatcctgtaa tcgctgcgac tgttccttgc cagtttcccc
     7021 aaccaagcgg ctgaaaaaca actgccagga ttcgcccgaa aaaggctaag atactttggt
     7081 cttcagaaac aggttgagca taaaaattat aagaagagct gaaccagatc acgatactag
     7141 aaacaaagat aatcgttcct gcttttttaa caaaagactt gctgcggtca taggtttgtc
     7201 ttaggaccgc tgaccattga ggaaaatgat atgcgggcaa ttccatgata aaaggagctg
     7261 tatctccagc aaacaaacgt gtttttttca agctgattcc agagaaaata attgcagtca
     7321 taccaagaaa ataggcagaa ggagctaccc aagattgatg aggaaaaaag gcccctgcta
     7381 ctaaggcaat aatcggcaac tttgcagaac aaggcatgaa ggttgtgacc atgatcgtca
     7441 aacgacgatc ttgctcgttt tcaatcgtgc gactggccat gacgcctggc actccacatc
     7501 ctgtagcaat cagcatagga atgaaggatt ttcctgaaag accgaatttc ctgaaaagtc
     7561 gatccatgac aaaagcgatc ctcgccatat atccgcagtc ttccagaaag ctcaagcata
     7621 aaaacaatat aatcaattgc ggtagaaatc ctaacactgc tcccacgcct gcgatgatgc
     7681 cgttcaagat cagctcctgc atccatcccg ctacttgcca ttcagctaac aatctgccaa
     7741 catgttctgg taaccatgta ccaaacaatg tgtcattgat ccaatctgtc cccattgttc
     7801 ctactgtttg aatagccaga taatagatca gccacatcac aaatgcgaag atcggcaagg
     7861 ctaaccaacg attcgttgca atctgatcga ttttgtcact catcgataat tggaaagaat
     7921 catttgacac aacacagagt gtacatagtt gggtaataaa ttcataacgt tcattgacca
     7981 gaatcgtctc actatcgtcg tccaatagtt tctctgttaa ctggatcagt tgctcgatct
     8041 ctttttgctg agaaactgct atctcgtaat cctctcttac cttctgatct cgttcaaaat
     8101 atttcattgc ataccagcgt ttcttgccgc tttgttctcc tagtacatct tcaatttcat
     8161 gtaaagctgc ttctaaacgt ggatcatatg tacagggaac aggaatagaa ggccgctttt
     8221 ttgcagactc ctgaatcttt tcgattagtt tttcgattcc ttgctttttc aatacgctga
     8281 ttggtaaaac tggtacacca agaccatagg aaagcttttc tagattgatt ttttttcctt
     8341 cttttttgat gacatccatc atattcagac caatgatcat aggaatgtct aaatccaata
     8401 actgcgtagt taaataaaga tttcgttcta aattggtagc atcaataatg ttgatgatca
     8461 gttcaggctg ttcctgaacc aaataatctc ttgcaatcac ttcttctggt gtataaggag
     8521 acaaagaata gatgccgggt aggtcctgaa tatgaatgtt cggatttttt ttcaacgatc
     8581 cttctttttt ttctactgtt acgcccggcc aattccctac atattgattc gaaccagtca
     8641 agagattgaa caagctagtt ttcccgctgt ttggatttcc gactaacgcg atattcattc
     8701 tttttcctcc ctttgtatca agattagttc ggcctctgac tttcgtaacg tcagttcata
     8761 ccctcgcact gtcagttcga ttggatctcc cagaggggct agttttcgga taactatttt
     8821 tacatttcgc gtcaatccca tatccattaa ccgtcgcttc actgcgcctt gtccgtggat
     8881 tgctacgacg atcccagtct ctcctgcttt taattgatca agagaagtcc agctttcaga
     8941 ggaagcagtc tttttattga caaagatttg acctagtatt tcccgactta ctgccactcg
     9001 atgacttttt accagtaaaa tggcattgtt gcctgataaa ttgacgacga cgattttact
     9061 tccggcaacg atcccgatat tttgcaaatg gcgttttgtt ttttcttctg tttcgattcg
     9121 ttcgacttga taaacgaaat tctgctttgc ctgcgctaag gtctgcatat tgattcctct
     9181 cttgtgaaat aaatcatgta ttaaaatcat tcattatatg ttctaatttt cttacgtgtt
     9241 acgcaaataa atgagaatca ttctcacata gaatactctc ggtaattgag aatgtcaatt
     9301 atacttgtga tattatgagc gaatctttag tattgataga taaaaatgag aaaaaaatat
     9361 ttcttcatag ttttccacaa ttttttctct attaaatttg ctattttccc acacgatact
     9421 tgttttcagc tcttttttca aaaacacaaa acttagtatt ccacagtttc cacaacacaa
     9481 tatgtagtgt ttgttgtttg acttttttac taagagtttt atactatcta ttatagataa
     9541 taagaatgtg ttagaggagg cactcaccat gaacgtaaaa atcttttcta aaaataattg
     9601 tatgcaatgt aagatggtca aacggttttt aacagaaaat caaattgctt ttgaagaagt
     9661 caatatcgat gaacagcctg aagcagtaga ctggttgaaa gcacaaggat tccaaagtgt
     9721 tcctgtcatt acttctgatg cagaaacagt tatcggattc cgtcctgatc agttgcgtaa
     9781 acttgccagc taacatgact aaaagaatca aaacgtgata catggatttg aagggcttga
     9841 gacagcacca gtttttgtcg caaacttcca aatccttttt taaagcttaa aaacaatgca
     9901 atgatttgca atcaagcatc atcaaatacg aaatgaaggt tgggcgtacg tatgagtctt
     9961 aaaaatttac aagatgtcag ttatttcaag ctgaataatg aaatcaatcg gccagtcaat
    10021 ggtcaaatcc ctttgaataa ggataaagaa gctttagttg ccttttttga ggaaaatgtc
    10081 aaaccaaata cgatgacttt tctatctatt atggaaaaaa tcaattactt gatcgatcaa
    10141 gaatatatag aagcagaatt cattcaattg tattcaccat catttattga aaagctatat
    10201 caatttttgg acgagcaaca tttttctttc aaatctttta tggctgctta caaattttat
    10261 tctcaatatg cgttgaaaac caacgacggt tccgcttatc tggaaactta tgaagatcga
    10321 gtagccttca atgcgcttta ctttgcgaac ggggatgaag aattagcact tgctcttgca
    10381 gacgaaatga tccatcaacg atatcagcca gctacacctt ctttcttgaa tgctggacgt
    10441 aaacgtcggg gcgaattggt gtcttgtttc ttgactcaag tcactgacga tatgaactct
    10501 atcgggcgct caatcaactc tgctttgcaa ttgtcccgta ttggtggcgg cgtagggatt
    10561 actttatcaa atcttcgtga agccggcgct cctatcaaag gatatgaggg tgctgcaagt
    10621 ggtgtcgttc cagtcatgaa attattcgaa gatagtttca gctattctaa tcaattaggt
    10681 cagcgtcaag gagctggcgt ggtttatttg aacgttttcc atccagacat tattgctttc
    10741 ctttcagcaa aaaaagaaaa tgcagatgaa aagattcgcg taaaaactct ttctcttggc
    10801 gtgatcgtac ctgataaatt ttatgaattg acacgtaaaa acgaagatat gtatttattt
    10861 agtccatata gtgtcgaaaa agaatatggt gtcccattct cttatgtaaa tattacagct
    10921 gagtatgatc atctagttgc aaatccaaac attcgcaaac aaaaaatcaa agcgcgtgat
    10981 cttgaaaatg aaatctcaaa attacaacaa gaatctggct atccttacgt aatcaatatt
    11041 gatacagcga accgcgagaa tccaatcgat ggcaagatca tcatgagtaa tttatgttca
    11101 gagattttac aagttcaaac gccttctgtg attaacggca aacaggaata tgaagtatta
    11161 ggaacggata tctcatgtaa cttaggctcg acaaatatcg tgaatttgat gcaaagccct
    11221 aactttggtc aatctgtgcg tacgatgact cgtgcattga catttattac agatgcatct
    11281 gatatcgatg tcgtaccacc gatccaaaac ggaaataagc tgaatcatac gattggatta
    11341 ggtgccatgg gcttacacac ttatcttgca aaagaacaaa tggaatatgg ctcagaggat
    11401 tcattagact ttacaaatat ttacttcatg ctgttgaatt attggacact tgttgaaagc
    11461 aacaatatcg ctcgtgaaag aaaacaagtt ttccataatt ttgaaaaatc agcttatgct
    11521 gacggcactt atttcgaaaa atacgtaact ggagaattcc agccaaaatc agaaaaagtc
    11581 aaagcattgt ttgaaggcat ttttatccca actgctgaag attggaatgc tttgaaacaa
    11641 gcagtaatga aagatggact gtatcatcaa aatcgcttgg cagtagcacc aaatggctca
    11701 atttcatata tcaacgatac gagtgcaagt attcatccta tcactcgcat gatcgaagaa
    11761 cgccaagaga agaaaatcgg taaaatttat tacccagcgc cttatctatc caatgagacg
    11821 attccgtatt acacttctgc ttatgatatg gacatgcgta aagtaatcga tgtttatgca
    11881 atggcacaac aacacgtcga tcaaggcatg agtttgactt tattcatgcg ttctgaaatc
    11941 ccagaaggca tttatgaatg gaagacaaca acgaagcaaa cgacacgtga tttgaatatt
    12001 ttaagacatt acgctttcca caaaggaatc aaatccattt attacgtacg tacctttact
    12061 gatgatgcag aagaaatcgg aagcaatcaa tgtgaaagct gcgtgatcta atatcataga
    12121 tagattcgct tattcatatt ttaattaaag gagcattctc atgtcagaaa catattatga
    12181 agcaataaac tggaatgaaa ttgaagatat cattgataaa tccacttggg agaagctgac
    12241 agagcaattt tggctagata cacgtatccc actttcaaat gacctagatg attggcgtac
    12301 attgtctcaa ttggaaaaag atacagtagg acatgtattt ggcggtttga cactgctaga
    12361 tactgtccaa tctgaaagtg gcatggatca gttgcgtaaa gatgtccgca ctccccatga
    12421 agaagccgtt ttgaacaata ttcaattcat ggaatccgtt catgccaaaa gttattcttc
    12481 tatttttagt acactgaata cgaaaaaaga aatcgaagaa atttttgact ggacaaatac
    12541 gaatccttat ctgcaaaaaa aagctgagcg tatcaatgaa atctacaaac acggtacacc
    12601 gctagaaaag aaaattgcca gcgtcttttt agaaacattc ttgttctatt caggttttta
    12661 tactcctctt tattatttag gaaacaacaa attggctaac gtagcagaga tcatcaaact
    12721 aatcatccga gatgaatctg tacatggaac ctacatcggt tacaaattcc aattaggatt
    12781 caacgagtta cctgaagcag aacaagaaac gttaaaagac tggatgtaca atcttcttta
    12841 tgagctttac gaaaacgaag aacgttatac agaagaatta tacgatccaa tcggctggac
    12901 agaagaagtc aagacattcc tacgctataa tgccaacaaa gcattgatga acctagggat
    12961 ggatccactc tttccagata ctgccaatga tgtcaaccca atcgtaatga acgggatttc
    13021 aacaggcaca agcaaccatg acttcttctc acaagtcgga aacggctatt tgttaggtca
    13081 tgtagaagcg atgaaagacg acgattattt gattggattg gaataaggat ttattttaat
    13141 tcaaaaattt ttcgaaaaat aaaaagagtt tagcgtacag atattttaaa tgtacactga
    13201 actctttttt atgcttattc acttgtttct tccagcactt ttggactgtc taacttttcc
    13261 gaaacatgtt ccattcttct caataaatag attttccgga tatttaatat tatcgtctta
    13321 atcactgcat agacaggtat gcaaatcagc atgcctaata ttcctgcgac acttcctgct
    13381 ccaatcaata ccaagatgat cgtcaaagga tggatattca ttgatttccc aaataacaac
    13441 ggcttgatca aatttccatc taattgttgg atcactaaaa tcgacaacgc catatacaaa
    13501 gcttgaaatg gagagataaa caaaccgaca atgattgctg gtaccgctcc aataaaaggg
    13561 ccgacatacg ggataatatt cgtgattccg caaatgaccg caagcaacaa gctataaggc
    13621 tgatcgaata ttctcattgc caaaaagctc attacaccaa taatcaaagc atctaagatc
    13681 gtactgctga tataggcaga taatgtttcg tttaattctt ttaccgtttg gcgtaactct
    13741 ttacgaatcg ctttcggaaa gaagtgagac aacgcatcaa taaatttgtg cccatccttg
    13801 aacataaata gcaaaataaa tggaacggta aataacaaaa taaaaaattg catcataaat
    13861 gaaatgattt ttgagacact tgcagataaa ctgactaata tgatattgaa caaattacta
    13921 agagataaat ttgctgatgc taattgttct tccaaattga aatttttaaa ctcttcccgc
    13981 tgcgccaatt catttaacca tttgcttgtt tcatctgcat acattggcaa gctctgggtc
    14041 aattgaattg tctgttccat taactgaggc actacattca tgacagttaa aacaactaaa
    14101 atgattacga ccaaaaacgt caataaaaaa ccgaacactc tcgggatccg atgcttttcg
    14161 agaaaaacaa cgattggatc gaacatataa tagataaaac cagcaacaag caatggtaaa
    14221 aaaactgccg aaagcatttt caatattgga ctaaaaatat tcggcatttg taaaataaag
    14281 tacacgccga tgatcgtgag tactagctcc actgtccaga aaaataattt tgattgttta
    14341 aaacgtgatt gcatacatct cctccaaggt cctttgatac ttctattcta tcatttaaca
    14401 aagagttacg cgctgagatt ttcatttgct taaaaaaaac aaaaaaaata aacatatttt
    14461 aatgagtaga cgagtactta aacaaaaaca cggtttggag tagtgacaaa aattatactg
    14521 cagtcccttt ttccaaataa acggtgttca aaagcttgca tcttcgtaat aagcccagcc
    14581 aaacgaaaaa tataaggaac tatttttatt tggctgggct tattacttga tgtaggacat
    14641 ctattttaca acctcagatc atttattgcg caatactgac taaatgcaac tttgtgatat
    14701 ttttgtgtac ggacaactcc tcgatcacat cggcataagt gagccctttt ggaagatcaa
    14761 tcgtatagat atttttgtac actcggtagt catcaacgaa ttttacgtca aaattcacat
    14821 cttcgattac gatatttttc tcttcaaaat aatcggtaag aaaatctttg gtttcttttc
    14881 tatggataaa ttggatttct aactttttag tcgtaggtac atggatcacg cgtttcacta
    14941 ctgtgagtgc aatagcaatc ccgataaaac tgatgatcgc aatggaatag taacccatgc
    15001 cgatggcgat ccctaatcca gcaattgccc ataaagaagc ggcagtcgta agtcccgtca
    15061 ccgattgttt cgtgacgatg atcgttcccg cgcctaagaa accgacacca ctcaccactt
    15121 gagcaatcag ccttgcttca tctgaacgaa tcgttccagc aagctgtgga tactctaaag
    15181 catctcttaa tgcaccacta gcgatctcaa cttggatcaa tgcaatgata catgctccca
    15241 tacaaactaa aatatgcgta cgcatacctg ctggacgatt tttatattgt cgttcaaaac
    15301 cgattgctcc gccaaatagc atagcgatcc ctaaacgtaa aataatctct ggaattgtca
    15361 ataccatatc attcatctaa acaccctcca gtccaatata tcatgtctga aaggagcaga
    15421 caaaaaaagt gcaattttta cctcttacga aaaaaggact gtgacaaaat ttttgtcata
    15481 gtccttttta tgaataaacg gtgttcaatc agctgaccct cggtattact caacaaacgg
    15541 caaaatatgg ggagctattt ttcctttgtc ttctctaata ctcaggtcaa aacgctgatt
    15601 tcacaacctc tttagcctac tattgaaaat tacaacttac ttgctgcttc ttcatcgatg
    15661 atcacaacaa cattgtcgtg attttgcaat acacttgctg gcaaatgatt cgttacaggt
    15721 ccttcaatca tccctttgat tgcatcagct ttgctttcgc caaatgccat caagacgatt
    15781 tctttacttt tcataattga accgattccc attgaaacag cttttgttgg tacatcttct
    15841 gctttatcga agaatcgttt gtttgcattg attgttgatt ctgttaagtc aactactgaa
    15901 gtcgttccgt caaatgaagc accaggttca ttgaaaccga tatgtccatt ttgcccgatt
    15961 cctaagattt ggatatcgat tggatgtgca tcgatgattt gatcatactt tttgcatgct
    16021 gcttccagat cttctgcttt tccatctggt acaaacgttt ctttgaatgg ttttttatca
    16081 aataattgga catccatgaa atgacggtag ctttgttcat ctgaaccgcc tagtccaacg
    16141 tactcatcta agtttacaga agtcatttca ctgaaatcca aatcgcttga agtcatttct
    16201 ttatataaag taactggtgt gcttcctgtt gccaatccta atacttttgc acctttgttc
    16261 ataccttctt tgatgatttc aaatgctttt tttccgcctt cttcagcgtt ttttacacga
    16321 atgatttcca tagtgattgc tcctttatat tttggtatag cccaattata gcattctttt
    16381 tttcttttta caataattag tatagaccaa attgcatttt tttcattttc ttgacaataa
    16441 ataggaatcc gtgttaggtt tcataagatg gttcaatcaa acaatccatg ttgcatgaaa
    16501 accaagcaag tgattcactg tggacgcttt atgtctgcag tttttttatg aaaaaggagt
    16561 tattatgagt atactgacat taaacaacat tacccagcaa tttggcgaca aaatcctcta
    16621 tgaaggtgtc gatctgcaat taaatgctgg cgaacatctt gggctgattg gacaaaacgg
    16681 agccggtaaa agtacattga ttaaaatcat cactggtgag atcttaccag atgacggaca
    16741 gatcaattgg caaaaaaata ttcacaaagg ttacctcgat caatatgtgg aagtcgatga
    16801 aacattaaca atcgaagaat ttctaaaaac tgcgtacgca aaagaatacg aaaaggaaga
    16861 aaaaattgct tttctttacc aaacatatag cgagaccttc gatgattcct tgctggaaaa
    16921 agctgggaaa ctccaaacgg aattagacca aggagtgttt tatcaaatcg acaccttagt
    16981 tgctgagatg agcagcgggt tggggatcga tgtcttaggt ttagattcac tgttgaaaaa
    17041 tctaagtggt ggacaacgtt caaaggtgat tttagcaaaa ctacttttag aaaacccaga
    17101 tgttctgatc ttagatgaac caaccaatca cttagatgat cagcatatcc aatggctgac
    17161 gcagtttctt caggattttg acggtgcgta tatcgtgatt tcccatgacc aagaatttct
    17221 tgatcagatc accacccata tcgcagacat cgagtttgga aagattacta agtatactgg
    17281 ccatctaaaa caagcattga aacaaaagga acaaaatcgc gaaagctatt tgcgccaata
    17341 tcatgctcaa caaaaacaaa tcgaaaagac cgaagcttat attcgaaaat acaaagccgg
    17401 ttcccgatca acgatggcaa aaagccgaca aaaacaatta gataaaatcg aacggctgac
    17461 gcctccagct tcagcaagta aagctgtctt tgattttcca ttctcgccta ttgtcacgac
    17521 attagcagtg gaaacaactg atttagtcat cggatatgat cgaccgctac ttgcacccat
    17581 caatttgacc atccgctttg gtgaaaatat tgccattcga ggcttcaacg ggattggaaa
    17641 atctacttta ttaaaaacac tgatcggtga gattgaaaaa ataagtggcg atttccactt
    17701 cccagataac acaaaaatca attacttttc acaagatctg tactgggaaa atccattaga
    17761 aacaccacta caatatttaa gcagtcaatt tccaaaagcg actatcaaag aattacgtcg
    17821 ccaactagct aaagcagggt tagttaatca attagcaagt gaacctctca ctacattaag
    17881 tggtggggaa caaactaagg tgaaacttgc acagttgaca atgaaccaag gtaatttact
    17941 gattctcgat gaaccgacga accatatcga tcaagaaacg aaagaaagct tacaagacgg
    18001 gatacagaaa tttccaggaa cagtaatcat cgtctctcat gaacaagaat tttatcaaga
    18061 tcttgtgcaa cgagtcattg agattgaacc aaaataaacg tttgattgaa tagttgaaaa
    18121 aaaggagctg tgacaaaagt tttgtcacaa ctccttcttc tgaataaatg gtggccaatt
    18181 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggagctatt tttatttggc
    18241 tattcttatt acttgatgca gaacagcttt tcacaacctc ttttcgtcta ttttttattt
    18301 tcgagatttc tctttccttt aaaaacaact gtttgaaaga agaacgcaca tccttgaaaa
    18361 acaagcccgc taaccgttac acctaaccag ccatactgct gccacattgt cgtccctagc
    18421 aatgaaccag ccgcacctcc aataaagtac ataaacataa atacagtatt atttcggttg
    18481 cttgctgctt cacctagccc ttgtactcgt gtttgattgg cgacttgacc aaattgattc
    18541 ccgacgtcta ataaaataat agctaaaagc aaaatggcca aatggcttcc gccaaccaat
    18601 aaagcaataa agcttattgt ttgcataaca agtcccatat acacgatttt tctttcagaa
    18661 taagaatctg ccagtcgtcc cacgataggt gcagcaactg ctccagacaa accaaaaatc
    18721 gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg gactgctgat ataaaaaatc
    18781 aacgtagacc aaaagatcga aaatgttcca aacatgaaaa atccactgac tgccgcttct
    18841 cttaatagac gttgagaagc aactaaatgc ggcaaacttt taagtgaatc aaggtatgaa
    18901 acagctgtac tactttgctg gacagaacgc ggcattttcc agtaaagcag aagtgtaaga
    18961 attccgactg cacataaagc aaataaatag atcatccgcc aagaaaaaag actggcaaca
    19021 ataccagata ccgtccgaga aagcaaaatc cccacgagta aaccgctaag caatgtaccc
    19081 atgaccttcc ctcttgcttt tggtcccgct agtaccgcac cgtaaggaat gatgatttgc
    19141 ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg caaataacgt aaacgaaggc
    19201 gcaaaaaaag cggccaacag agagacggca gataacgctg ccattcgaat gatcaatttt
    19261 ggacgatcca ccacgtcccc taaaggaact aaaaacagta accctaacgc atagcctaat
    19321 tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa cagataaact agctgctact
    19381 tttgttccaa tcggctggat gtaatacata ttggcaacaa caacgccaca agtgattgat
    19441 aatagtaaaa taagcgaagt tgttaaaggt tttttttcat tactcttcat tgatttcccc
    19501 catttcctat cttagtttac aacttatgga aaagattgga aatagaaaaa tacaactatt
    19561 cgccacactt attatttgtg cttttgtttt ccttgttttt tcttgcgatc tttataaatg
    19621 aagacaacta accctactag cacagtaaca cttgcaacca acgtgactgc agttttgtta
    19681 tatccgtgaa acagagcttc tccaccacac gcatacaaca cagacgaagg gatgactcca
    19741 atcacgatcg acaatatgat ttgctgtaga ggcaatttca tcgcatctgc agcaaaattg
    19801 accatagaag atggaataac cggtatcata tagcctaaca ttacacctat ttttggatgt
    19861 ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg ttttcttatc taaaaatttt
    19921 aagcgatgca taaggaaaat tgcagtgatg ttaccaagag cattacccaa aatgttgatg
    19981 atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga ctccaactac tgaagtagga
    20041 atacctggaa cagcacacat caccgctgtc aatacgatca agagtatcga tgcaaaagtc
    20101 ccatgcgaac gaatatgtgc gagtagctct gccttactcg ctctcggatt taaaaataat
    20161 tggatgtctg tctgatattc taaatacaac cgatagccca ctaccagtat cagaatgatt
    20221 cccaacgaga tcaatatcaa tcggattatt ttctttttca tatttcctcc tgaaaaaaat
    20281 ctatattcat taaataatag tttaacataa aaacaaaaaa gcaccgaaat ccaacatagg
    20341 atctcagtgc ttttaaatag taaaaattat ttaacagtta cagatgcgcc aacttcttca
    20401 agtttagctt tcaattcttc agcttcttct ttagatacgc cttctttaac tggtgcagga
    20461 gcgccatcaa ctacagcttt agcttctttc aagcctaaac cagttgcttc acgaactgct
    20521 ttgataactt taactttttg gtctccagct gcagttaatt ctacagtgaa ttctgtttgt
    20581 tcttcagcag cagcagcgcc accagcagca gctacagcta caggagcagc agcagatacg
    20641 ccaaattctt cttcaatagc tttaactagg tcgttaagtt ctaggatagt tgcttctttt
    20701 aactcagcaa cgatgttttc aatgttcaat gccattataa tttcctccgt ttaattgttc
    20761 ttttttattt aatgctttat gcggcaatta agctgcatct ccatcttctt tgtctgccac
    20821 tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt agtacagata gcaacataga
    20881 tagaagtcct tcgcgatttg gcaattttgc caatgctgtg atttcttcca atgaagagac
    20941 tttaccttcg atgataccgc ctttgatttc taatgctttt gcttctttag caaattcgtc
    21001 catgattttc gctggtgcga ctacatcttc gttactgaat gcaacagctg taggtccagt
    21061 gaatacttcg tccatgcctt caaggccagc ttttttagct gcacgtgaaa gaatagaatt
    21121 tttgataact ttcatttcta cgttagcatc acgaagttgt ttacgtaaac gtgttacttc
    21181 atcaactgtt aatccgcggt agtcaacgac tactactgat gcagctgctg taaatttttc
    21241 gcttacttca tcaacgattg ctgctttttt tgcgataatt gcttcactca tttttgagtt
    21301 tcacctcctg aattttgatg gaagagtttt ttgcactgaa aaggcaagtc acttttatag
    21361 ttactttgct caaccagtat tattcatcac ctattcactt cgttcatagt gattctactc
    21421 gctttgctcg aaaggaaact cctttcgctt tgctcaacca gtattgttca tcatctcttc
    21481 acttcgttca tagtgattca caataaaaaa ctctatgcca ccgcagacat agagggacta
    21541 ttgaatatat tcaataaacg tcctcggtag gaaattaagg cttgatgcca cctactgtct
    21601 tcggtacaag ttaattatta acctcaacta gcttagcatg ctaaactagt tgagtcaaga
    21661 attatttttc agttggatca atcaacattc taatatttaa ttagaaagaa gcttggtcaa
    21721 catggatacc aggtccaaat gttgttgtga tcgttacatt tttcatgtat tgacctttag
    21781 ctgctgatgg tttagctttt agcaatacat catggattgc tgcaaagttt tcaactaatt
    21841 tttcatcgtc aaatgaaact tttccgattg gtacatggat atttccagct ttgtccacac
    21901 ggtaagttac tttaccagct ttcacttcgt ttactgcttt tgttacgtcc attgtaactg
    21961 taccagtttt agggtttggc attaagcctt ttggtcctaa tactcgtcct agacgaccta
    22021 cagtagccat catatcaggt gttgcaacaa ctacatcaaa gtcaaaccat ccgttttgga
    22081 ttttttgaac catgtcgtcg tcacctacga aatctgcacc agctgcttca gcttcttttg
    22141 ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt accagttccg tttggtaata
    22201 caacagcacc acggatttgt tggtctgctt ttttagggtc tacgttcaaa cggtatgcaa
    22261 cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt tgctaaagct actgcttcgg
    22321 caacaggata aactttactt gaatcaactt tttttaatgc atcttgcatt tttttgctct
    22381 ttttagccat tttggttcct ccttgattgt ggttataacg gttgtacctc ccacgtgttc
    22441 tatatctttc aatatagagc caactgagaa gctgctgtct tagggtgtat agattattct
    22501 acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc gcattgctgc ttcaacgtta
    22561 gctgcgttta ggtcttccat ttttaattca gcgatttctc ttacttgatc gctagaaact
    22621 ttcgcaactt tatttttgtt tggttcgcct gaaccttttt cgattttagc tgcttttttc
    22681 aatagaacag cagctggcgg tgtttttgta acgaatgtga atgaacggtc ttcgtataca
    22741 gaaataacaa ccggaatgat caaacctgct tgatcagctg tacgagcgtt gaattctttt
    22801 gtgaatccca tgatattgat acccgcttga cctagtgcag gacctactgg tggagctgga
    22861 gttgctttac ctgcaggaat ttgcaattta acgatttttt ctactttctt tgccacgaga
    22921 catacctcct taagtccgtg atgtggttaa ttggagatgc atatttctcc tcccacgttt
    22981 tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac aatgcacact ggtacagtat
    23041 agcaaattta tttgtttttt caagattttt ttctgctatt tttacatttt gcttgaataa
    23101 acgctaaatt tattctaaaa aaacaacaat ttttcgttct catttcttct tttatgttac
    23161 acttgcgcta gcatggttca acagatgaaa gcaaacaata acacggtttt ttagaccctt
    23221 gtacaaagat tcctacataa caaaatgagg tggagaaaat gaaagattta aggtatagaa
    23281 gtgtctttga tattattgga cctgtgatga ttggtccaag tagctctcac actgccggag
    23341 ctgctagaat cgggaaaatc atacgtagta tatttggcga acagcctgat tctgtagata
    23401 tttatctata tgaatcattt gcaaaaacct atcgggggca cggaacagat attgcattag
    23461 ttggcggcct gttagggatg gaacccgatg atgaacgttt agctgattct ttgaaattgg
    23521 catatgaagc cggtatggaa gtcttgtttg ttcctaagag tgaaaaagcc gaacatccaa
    23581 actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct ttcagtcact ggcatttcga
    23641 tcggcggtgg caatatccag atttctgaat tgaatggatt caaaatttct ttaagcatgg
    23701 gtactcctac cttgatcgtt gtccaccaag atgttccagg tatgattgct aaagtgacta
    23761 gtatactttc tgagaccaat acgaatatcg gcactatgac agtgacacgg gaatcaaaag
    23821 gtgaaaatgc cattatgata attgaagtag atgatcctca agtagaagaa acggtaaaaa
    23881 gactcaaaca gctgccaaat atcgacagcg taaattattt cgattaagga gctaaaaaaa
    23941 tgtttttatc gattgaagaa ttagtagatc aagcaaaagc ttatccaagt gtagcagaat
    24001 tgatgattgc agttgaaatg gaaatgagtg gccgttcccg tagtcacatc atcgagatca
    24061 tggaaaaaaa cctctcagta atggaacaat caatcaacga gggaaaagaa ggtgttacct
    24121 ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga tgattattta aatgaggcag
    24181 actttttaag tggagaaaca atcttaaaag ctgtacgcaa tgctgtagct gttaatgaag
    24241 tcaatgcaaa aatggggttg atttgtgcta caccgactgc cggaagcgcg ggtgttgttc
    24301 ctggtgtgct gatggctgtc cgagatcggc ttgatttgac acatgaacag caattggaat
    24361 tcttatttac cgctggcgct ttcgggttag tcatcgcaaa caacgcttct atcagtggtg
    24421 cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc aatggcagca gcagctttgg
    24481 tttgtgcaaa aaatgggact cctcatcaag cagcacaagc agtagccatt acattgaaaa
    24541 atatgatggg actgatttgc gatcccgttg caggcttagt tgaagtcccg tgtgtcaaac
    24601 gtaatgcttt aggctcttct caagctttta tctctgctga tatggcatta gcgggtatcg
    24661 aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta ccaagtcgga cgtcaaatgc
    24721 cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt tacgccaact gctaaacgac
    24781 ttgcggaaga gattttaaat aagagggcgt gataaagctc cttaatttcg agtattaaga
    24841 gattagttat cttcataaca aaaaacgaag agaggactaa ttgcccttct cttcgttttt
    24901 tattaatcca tcaatttcgt attttttact tcatcgatcg tttttgttcc tgcaagttgc
    24961 atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag atgttactcc ttctgcccca
    25021 cctagattca acccataaat cactggtcgg ccaatcgcaa ctaagtcagc accacttgcc
    25081 agagctttga agacatgctc tcctcgacgg acaccagagt cgaaaatcac tggtacacgt
    25141 ttgttgacta cttcggcaat ttttggcaat acttcaaagg acgcaggacc tccatctagt
    25201 tgacgtccac catgattgga tacccagata ccatctgccc ctgcagagat agcgattact
    25261 gcatcttctg gcgattgtat tccttttacg ataactggaa gatgggtgat ttctttgatt
    25321 gttttgatgt catctggtgt caagccttgt tttgcagctg cataaatttc tgcgattcct
    25381 tttccttcgc cattaccact tgcactttgt tcgctatacg cagctaagtt aggcattggt
    25441 aatggaaatt ggaattgatt gatgacgtct tcttcacggt aaccaccgag cgttgaatct
    25501 gccgttaaaa tgatagcttt agcccctgct tctactgctt tgttcaaaat gaactcattg
    25561 aatctgtcgt ctttactcat atataattgg aaaaactgcg gtgcgcctgg cgctgcatcg
    25621 gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg aaaagattga tccagcagca
    25681 gctactcctt tagctgtatc agcttcccct ttttcatgtg ccaatccttg ggccgctgat
    25741 ggcgcttgga taatcggtgt atctaattca attccgaaaa cagatgtatg caaatcggct
    25801 gagtcgattc cgcgtaatat ccgtggcatg attttctttg tattaaaaga agtcgtattt
    25861 tctttcatcg tccactcatc ttcagaacca ccgcgaatat agccaaaagc tcctgcttcc
    25921 atgcgctctt ttacacgacc ttctagttag gcaatgttta caatatcgat tggatgttct
    25981 tcattacttg tatgataagt catagtagat ccttctttca tcttcttgat tacatataaa
    26041 tcatactaaa cttacaaaaa gtaagcaaac atttactctc agatactttt atcgctattc
    26101 tttccaaata aatcgactta caagctgatc tacttcttgg ttttcatgaa gcatactatg
    26161 agaggcattt tcacctgtca ctactttttc ctctatttca tttccgcgtt gcttcaacaa
    26221 cgcatagacc gctaatgcac tgtttaacgg tactgttcca tcactcaagt ctgtttcatc
    26281 tagttgacca gcaatcaaga aaaatcgagt ggttatagga atattaccga tcatttgctg
    26341 gtaatcttga tagcggttag aaacgacagc aggccccttt gctaactcat ctgtcagaga
    26401 ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca attgctgtta atgtctgaac
    26461 ttgtggcaat gacgtatcct gtccatacgt tcctaaatag cgtagtgcac taactcctcc
    26521 catggaatgc cccactatgt tgactttatc tatgccgtag ttttctttca gatacaaaag
    26581 acaagttttg atccattctg cttgattcca ctcgttattt ttattatctt caaacaatac
    26641 ttggaccatc ggattgtctg cttttccaga caaatctcct gttgcttgta tttgtccatc
    26701 tgttcctact gttaaaacca attcttttac tgcttgctga tttttttcca aacgccgcaa
    26761 catcccttta aaggaatttt ctgtcccgct gtagccgtgg aaaaaaagtg ttggaatagt
    26821 ggttttttct atcactttac tggattctgc cgttttttct ttatcagaaa cttcagtagt
    26881 atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa ctgataagga ctaaagctgc
    26941 tgcagttgca cgtttgcata aatttttgat tggttctgtc atacacctct tcctttccat
    27001 atagaaaaaa gaaagacttg aaaggccaat catatgatcg acatttcaag tctctttatt
    27061 aatcgatttt atctacttga tcgaaatcaa gttctgtact ggtttcacgt ccaaacatat
    27121 cgatgttgac tttcaatttt tctttttctc cgtcaacttc tgttacttgt ccttctaatc
    27181 cagcaaatgc gccttcgatg attttcacga cttcgccaac ttctacttct agatcatttt
    27241 gtcgcaggct cataccgata gaacgcaaga tatgattgac ttcttctgga agtaatggtg
    27301 ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt cacaccaggt gtgttgcgga
    27361 caacatacca tgaagcgtct gtcatgatca tttcaacaag tacgtatcct gggaatgttt
    27421 tatgaacgat ttctttttct ttcccatttt tgacttcagt ttctttttct tcaggaacaa
    27481 cgacacggaa aataaagtcg gccattttca tactttgcgc acgagattcg atatttgctt
    27541 ttactttgtt ttcatatcct gaatatgtat gcaagacata ccagtttttt tcaaaagatt
    27601 ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc tcagtacacc cgaagttttt
    27661 ctttcgcttc attatagcac taaaagtacg ttgtggctag cgagttattt taaaatccag
    27721 ccaaataagc tttggatggc tgtatccatt acgtaaaaca tcaatccgaa aataatagaa
    27781 gtttcgatga ctactaacgt atctttacgg agttgttttc tgcttggcca agttacttgt
    27841 ttgatttcat cttttacgct ttttagaaat ttcatgagac aacctccctt gctactgttt
    27901 atttcgtttc tttatgtaat gtatattgat tacaatattt acaaaactta ttgatttcca
    27961 aacgttcgcc gctttttcct tcactgactg attttgtgta gttgcgagac ccacaaacgg
    28021 aacaagcgag tgctgctttt ttctttgcca tttacttctg cccccttgct gagaatcaga
    28081 cttatacttc ataacagtat cacgtaactt tttacttgtc aatttgtgaa ataaactatc
    28141 tattgttttg gataaaatta tgatatgatt aatttgaaat taagctaaaa tgagacaatt
    28201 ggaggtatag cgatgctatt aaaaacactt gtatataaga aaaaagatct gacaaccgtt
    28261 acagagacta gtacattaga agaagcatta aaaattttag aaacttccgg ataccgttgt
    28321 gtgccgatct tagattcttc aggtaatatt ttccgtggaa acatttacaa aatgcatatc
    28381 tatcgtcata aagcaaatgg cggtgatatg acgcttcctg tcactcatct tttgaaaaac
    28441 gctacaaagt tcatttacct ggatacttct ttcttcaaag tattctttac gatcaaagag
    28501 cttccttata ttgctgtcct tgacagtgaa aatcattttt atggtatttt gacacatagt
    28561 acgttgctta atatgctctc tcaatcttgg agcgtagaac aaggaagcta tgtactgaca
    28621 attgcttcca caggaaagca aggagatttg gctgctattg caaaaatcat ttcaaaacac
    28681 agcagcattg ccagctgtat tactcttgat atcgaaaaag acgaatacat tcgacgtaca
    28741 ttgatcacgc taccggctaa cactgaagcg aaagtatgtg aaacgatcgt caaccactta
    28801 gagaaaaaga attataaagt ggtagaaatt gaaaatttgc aaaaccaaga agcataatca
    28861 aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt cgctttttat aattagacac
    28921 gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa tggtgctgaa acagccatga
    28981 cgataaactc acttaatgca agcgtgccat aggttgtcca aaatgcttgg ccacctgttg
    29041 gaactaacat ataagcaatc agtcccatgc tgatggtaaa gaagatcgtg ttcaatacta
    29101 aacgcatctt tacatttttc actttgtttt gcaataaagc tgtcaggcct aaagaaagca
    29161 atgtttggcc gccaccaaaa accacatcca tgataccaaa accaaagaag gcattataga
    29221 cgatgacccc gcctaaaact ccccaaagca atttacggtt aaagacaact aaatgattca
    29281 aactttctga aatacgaaac tggatcggcc ctgaggatac aggcgcaact aaaatagtta
    29341 acgctagata aagagccatg atgatgccat ttacaacgat tacggataac cgttgtctgc
    29401 ttttcgtttt ttgcattttc tctcctcttt tctcctagtt tttttaacgc gggatggttg
    29461 atgaaccgcg gttatgtgaa acaaacaata attataacag acttttcatt tatttcaatc
    29521 cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg taaacgtttg ctaaaaatcg
    29581 tgggatatgg tataaagtaa gtgtaagggg tcataagaat tcccagtaaa gcagacgaag
    29641 taggatctgc aagaacagaa ttgttgtgaa acttaaataa ataaaaaagg atgtgggaat
    29701 ttgagcaacc tcttactaga gaacggtcta gcaagcagct gaagatctaa gtggacatgc
    29761 cgaaccttgg aacttctcct tacgtaagta atggatcatg cgcttgctag gcttttttgt
    29821 gtttttcggt cttcggacct ttttcatttt cctagacttc tgctataata accaagaatt
    29881 aattttgtaa cagatttttt tgaaaaaaca agtttctttc attcaaaaaa tagaaaaagc
    29941 ttgctaatta ttggttaacc agcaaacagg agtattaaaa tatcttgttt tattgaatag
    30001 caggaggaaa tcatgaaatt ccattatcat atattgatca atccatcggc aggcagcgga
    30061 aatggctata aagtagcaga aagaatttta ccggtattaa aaaataagca tattgactat
    30121 acacttcatt atagtgaata caaaggacac gatgctgaga ttgctgaaac tttggcaaaa
    30181 gaaacattga ttccttggat agaagaagaa cagaaaacaa tcgatacttt cccccttttg
    30241 atcatcgtag gaggtgacgg caccttacat caggtacttg atactttcta tcagatggaa
    30301 gtagaattcc cagttgctta tattccagca ggttctggag atgattttgc tcgaggggca
    30361 ggccttccaa aaaatcccaa aaaaggactt cagctgattt tagcagctca atcaccagaa
    30421 aaagtccaca taatggctta tgaagaaaaa ataagtgaaa aaaaaggaat agctgttaat
    30481 aatttcggta taggattaga tgcagctatc gtccatgcga caaatcattc ttccacaaaa
    30541 aagcgattga acaaatacaa cttaggatca ttttcgtatt tattctctat tctccgtgct
    30601 ttattcactc aaaaaggatt tccaattctg gtagatgcag gaggaaaaca attaagtttt
    30661 tcaaacgcat ttttatgtac agcaacgaag catccttttt ttggcggagg aatcgcgatt
    30721 gttccaactg ctgatgcgtc taaaccagtc attgattttg tcgtggtcga acgaatcaat
    30781 ttctttaaaa tcttgtggct cattctttta cttatacaga aaaaacaatt gaaatcgaaa
    30841 tatttccatc actacacaac aaacagatta cgtatcgttt caacaacacc gcaacatggt
    30901 caagaagatg gagaagaaat ggaaaaacga ccttttgata tcacaataac cacaactaaa
    30961 cagttgtttt ggtgttaata aaagaggttt tgtcagaagc tacatctgga acaccgttta
    31021 ttcggaaaaa aggactgtga cacgactttt gtcacagtcc tttttttact atgcagttaa
    31081 ttctttgccc aattgggttg ccatcgatag tttctttctt tacccaaaat aaattggatc
    31141 attgaaaaca ccgcagcaaa agcaactaag acacaaccat tgaaccaaaa agattctgca
    31201 aatgtttgat tgtaactgag tgtttcactg atcaagttat tattttgaac aaaccaatat
    31261 gttggtgtga atgccgctat tttgctaacc atttctggca agtattctgc tggtacaaac
    31321 accccaccga taaagcaact gcctgtgata aaaatatctt tgatcccacc gctggtttct
    31381 gagtttttta ccaatgaagt gatacaagcc gaaaagctaa tgatcggcaa taaaaacaag
    31441 aaagtattta aaatatgata ccataccgtt atatcccacg aggtgtggat cactaacaga
    31501 accattagaa gtaagaagcc actgaacaac agcagcgagt aactaatcaa actaaaggtt
    31561 actttgcgat tcaagtttcg tttggtaatg ggcgcactct ggtttcgttt actgatctct
    31621 ttgcgattaa atgctagatt gatgacgctg aaaccgctaa aaatggatag aaacaaccca
    31681 taagacaaga aattaaaggt tgtgcccgtc aacagctttt tcatccgctg actatatccc
    31741 gcacttagct caacttctcc agtaaccgag agtgccattt gtgtctgttc catagctgca
    31801 tcgattgttt tatcttcttc ttgaaacaga atcagtgttt gcaaaaaggt gttcacttgc
    31861 tgtgtaacaa ctgttttaga aaaggcatct gggcgactct gtatcttgat cgtcggcatc
    31921 tttcctactt cgacttgttc ggtaaagtct gcaggcagat gtaagataat gtttacacga
    31981 ttaaaataaa gagcatcatc gatttctttt tgtgaggttc catccaatgt aacgactgtt
    32041 tccttgtcat tcaaatattt acgaaggcca tcgactacag gcgactcttc ttcactaatc
    32101 aaggcgattt tcgcatcttg cagagaggta ttttcaacat caatattttg cgcaaaaaag
    32161 aatgtggcaa caacagtgat aattacacct atcagtaaat tcgatttatt tgctttaagc
    32221 aatgtccaaa tcgttttata aagcgtcata acgcacccct ctttccatcc aagcatttcc
    32281 taacgcaaaa aggaaaataa atccgatcaa ccatagaaga ttggtataaa aactagccaa
    32341 atcagtgtag aaataaagtt tatacaaact ttcgctgatc aagttgacga gatttaattg
    32401 accaacaatc ggaatatgaa gatcaatcca atacttcact tgttccgacc ccatcatacc
    32461 agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg ctatcctttt gcaccagatt
    32521 catttttggt atcaaattac caaccaatgt acctaacaaa atcgcacaca acgaccccag
    32581 agcggtaaca atcatcaacc attgccaacg ttcaccaaag tctacttgat agataaaacg
    32641 aaagacagct aacataatat aaatctctgt caaaacaatc gtaaagcttg ctgccaaatt
    32701 agcgattgaa actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga
    32761 ttgatttgct tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc
    32821 cattccaatc agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac
    32881 ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc
    32941 cacttctgcc actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt
    33001 tttcatcaac gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc
    33061 aatgagataa aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga
    33121 tagttcctgt acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt
    33181 aaaattttcc gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc
    33241 gagattgcca ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat
    33301 tggaaaggcc aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata
    33361 tttataaaaa tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa
    33421 tctctaaaaa gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct
    33481 tttcttcttg taagattgta agaatcggca acaatactgt atttagttca gtagttgtta
    33541 cttggagctg acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg
    33601 ttttctggga gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt
    33661 agagattctt tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg
    33721 caaagaattt ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga
    33781 ttcagctcct tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc
    33841 ggctcatcta aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga
    33901 cgtttcaagc caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact
    33961 aattgaatca cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg
    34021 caaaaataat cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga
    34081 acgaccccaa tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat
    34141 ttaatggttc ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc
    34201 ccactgccgt ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa
    34261 tgattcaatg ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc
    34321 atgaaaaact ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa
    34381 ctagtgacga ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca
    34441 taaatctcgg ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt
    34501 aagtcaagga ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg
    34561 tgtcagttat ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt
    34621 atgtgccttt ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc
    34681 tgaagaaagc caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg
    34741 taatcatccc cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt
    34801 acgaaaaaaa gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac
    34861 tcattgggaa aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga
    34921 aacacaatta gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga
    34981 caatgtcggt catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa
    35041 tcaagaacca aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat
    35101 ggatcggatt cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc
    35161 cttagagttt ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga
    35221 aggattgaat gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa
    35281 cagtatcaaa catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt
    35341 ttatcgcttg caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat
    35401 tggtttgaaa acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca
    35461 agaaaaccag caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca
    35521 gtagtcatta ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca
    35581 cgaaagagat ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg
    35641 aaacccatcg tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg
    35701 atgctgccaa agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat
    35761 tcaaagatga agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca
    35821 aacagaactt gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat
    35881 ttggtaatga aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata
    35941 gtcatgctta tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg
    36001 ggaaaaacaa taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt
    36061 acatcagcca attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt
    36121 tttatcagca tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg
    36181 catcattcag gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga
    36241 ttgaaagaat caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg
    36301 cggaaaaata ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg
    36361 ttctacatca ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa
    36421 gtcattgcca gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa
    36481 gtaacgcccc ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc
    36541 taaccaacga tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat
    36601 aaaatcgtag ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat
    36661 cacttctgct gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc
    36721 ttctccaaca aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt
    36781 ttcattcgtc ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt
    36841 tgtatagttc ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag
    36901 tttggtccgg agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt
    36961 catccggctt gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa
    37021 aatcaacagt tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg
    37081 ctgatattga tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg
    37141 gattgagcaa caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc
    37201 atttgcatca aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat
    37261 gatacccgtg atttctccca tatctccatt gaatacattc aattcgggag tattgactaa
    37321 ttggaggact ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc
    37381 aggtttccct ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg
    37441 tccccgatac attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc
    37501 tttttccaca attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga
    37561 acgatctttt tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc
    37621 aagaggaata atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg
    37681 aatttgttcg atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg
    37741 atctttgtct cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt
    37801 agccagccat gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc
    37861 tttcgtactg actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt
    37921 actggcaggc agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc
    37981 aagtagaata ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa
    38041 ctctgcaaaa agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc
    38101 tgttaaaata aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc
    38161 atactgtatg ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg
    38221 ataatggatt tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc
    38281 agaaaagtac aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc
    38341 aacaagttgg ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt
    38401 ttctaaaata cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt
    38461 ttgaactgca tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc
    38521 tgctgcaata ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat
    38581 gtcttcgact agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc
    38641 ctggtagata ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac
    38701 ttgatccatc ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt
    38761 caaaccggag atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc
    38821 tcccagcaat tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct
    38881 ggaaagatac gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag
    38941 tttaagctgc cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc
    39001 attttcttga atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc
    39061 tgtggtttca ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa
    39121 aatcgcggca actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct
    39181 ccttcttcta tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc
    39241 atactttcag ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac
    39301 tactattgaa caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt
    39361 gaatggctgc agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt
    39421 gggcaaccgc atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc
    39481 cgtgaaaagg agaaggatca tacagatcta aatgattacg caaattcggc agattatccg
    39541 gatatttctg gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc
    39601 ttagcttatc tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg
    39661 ctgtatcacg tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta
    39721 cctttttccc aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat
    39781 tgctcccttg aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata
    39841 gttccatacg cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat
    39901 ccagcttgtc attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa
    39961 gtgcttcata cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat
    40021 cattcatagg gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac
    40081 cggttgtttc ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac
    40141 tatatacttt atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta
    40201 aaaaagcttc taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg
    40261 cccgttttag atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag
    40321 tattcgtggc tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa
    40381 ataaaacgat atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct
    40441 gacaatttga tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg
    40501 ttacgttagt gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa
    40561 tgaaaaaaca acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat
    40621 taaaaactgc tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta
    40681 atcttcttca aaggattctt atataagtct gctttgattc gctcttctac ctgccattct
    40741 aaaggtgaca tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt
    40801 acacgctgac gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg
    40861 gaataactct gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa
    40921 ttttcttgtg gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct
    40981 tggtaatgag aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc
    41041 aaattggtca aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag
    41101 actccttctt ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt
    41161 tcattcaaga agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa
    41221 tgctgcgcaa tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt
    41281 ggaacgaaca ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt
    41341 ccttttttgg aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt
    41401 tttacttctt cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg
    41461 cgtcctcggt caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta
    41521 gatgctcact ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata
    41581 aaggagaaga ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag
    41641 caattgctag aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa
    41701 ccccgagtac tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg
    41761 taatgacgat tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa
    41821 tcatgcatac tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt
    41881 gtcttactcc ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag
    41941 aagggttgca gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg
    42001 aagcaatcga ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg
    42061 ctgacaaaac tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg
    42121 gccgcttaat cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta
    42181 aagaattgaa cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt
    42241 ataaacaaaa aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta
    42301 cataaaatag cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc
    42361 gctcaaagtc gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat
    42421 tagcatcggg attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat
    42481 ccctatgaca aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt
    42541 gatccccgta acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac
    42601 aaatccatga tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg
    42661 ggttccagca ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc
    42721 ttcacttacc aagtcttcat agtgactgat taaagaagcg gtcgacatac ctaaaaacaa
    42781 cagagtgatc agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc
    42841 ttccacattg accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc
    42901 gatgatatca tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag
    42961 agctaagaag tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg
    43021 aactgaaagc actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat
    43081 taggtcgcgc agagaaataa cccctactag ctgaccttct tgattaacga catagatgta
    43141 atagattgtt tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt
    43201 ttgattggct acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc
    43261 gtaatggaga agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt
    43321 agcaatttgc tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc
    43381 agaaagcata tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc
    43441 gttgtcttct tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata
    43501 gatcagctgc ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg
    43561 catctctaaa aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa
    43621 ctgggcaaat ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat
    43681 tcttcaaacc gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc
    43741 aactcttctg taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt
    43801 attcccagat ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg
    43861 gaaaaatgaa ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg
    43921 atttctgtta gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag
    43981 accattcgct tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt
    44041 gccgataaat ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt
    44101 tcacgaagtt gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa
    44161 ccagatgtat cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt
    44221 tttttataat aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga
    44281 atagacgaaa cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata
    44341 tcgatcggtg tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg
    44401 gttaccttat catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg
    44461 atttgaccgc cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt
    44521 aaaaaatatt ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca
    44581 atcctcacta ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc
    44641 aaaatggatc cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac
    44701 attcaattga tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca
    44761 ttcatcatgg gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga
    44821 agcaatctct gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact
    44881 tttattatat gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact
    44941 ttcaaacagc tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat
    45001 cgttgattca ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg
    45061 aacatccagc aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag
    45121 ttcttccagc tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc
    45181 caagaaacgc acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc
    45241 gtctccgccg actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg
    45301 ttctaaaagt gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac
    45361 gatcgctact ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat
    45421 agtagacatc atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt
    45481 cttcacggat actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa
    45541 gtcgttcact catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa
    45601 tcgttgccca gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct
    45661 caccgctgat cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg
    45721 cttttttatt tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga
    45781 tgatctcaac aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa
    45841 tatcagacat ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta
    45901 caatactatc gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt
    45961 gttcacggaa ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt
    46021 catatggtgc taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa
    46081 cattcattgt ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg
    46141 accaaaaaaa gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc
    46201 ttgaaatcga atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact
    46261 atcaattaac agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg
    46321 aacagctgaa gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat
    46381 atacattaaa aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag
    46441 aagaagcaga agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa
    46501 aactttctga tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa
    46561 aacgggctga attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca
    46621 atctacacga ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt
    46681 ttataaattt tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac
    46741 gtatgcttga agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt
    46801 tttacttttt tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat
    46861 ctattttggg ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat
    46921 actgtttcga aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat
    46981 caaaagaaaa gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg
    47041 tcatgcaaca aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa
    47101 tgacttattc tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc
    47161 gccgtttttt gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag
    47221 aacttgctga atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc
    47281 gtcagtctga cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg
    47341 gcatcacaaa acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg
    47401 ttttagtaga agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa
    47461 cagataactc atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc
    47521 attcaagaac tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac
    47581 aagagagccg acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat
    47641 aaacgatcga tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct
    47701 tcaatatatg cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa
    47761 tcgctgttac ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt
    47821 agtattttct tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag
    47881 taattataat aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg
    47941 actgctggac cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta
    48001 gtcaatggca cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt
    48061 gtctggcgga aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt
    48121 acacgagggt ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc
    48181 gaagcaattt ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga
    48241 gtgaaataac tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc
    48301 gtatcatgcc aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca
    48361 ccgcttcgtt tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct
    48421 acgatggcca tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct
    48481 tcttcgtacg taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg
    48541 tgcagttttt ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc
    48601 aaatgcttgt ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg
    48661 ctcaaagacg cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct
    48721 tttatatgtg tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac
    48781 aatcccttaa atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac
    48841 acgccatgag ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga
    48901 tctgcattat tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca
    48961 gcaagaagag attcctgctc agcagataag acatgggctc gattatctac tatttgctgg
    49021 atataatggc gatagacttc caattttggt tcttctttga aatattgcca aatctggtca
    49081 tctgacaatt gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac
    49141 agactgcttg ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt
    49201 ttaaggtgcg catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca
    49261 atcgcattta aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt
    49321 tttaactctt ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc
    49381 ttggtcaagt cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact
    49441 tccatgaaat ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta
    49501 acatccacat gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt
    49561 ttagcataac taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta
    49621 ctattttttc aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc
    49681 ttcatcaatc ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga
    49741 gttgaaatca ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa
    49801 aacctgttct tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg
    49861 caaaagatgt ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt
    49921 gtgaataagt tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt
    49981 catttggttc attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa
    50041 agtccagcag ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata
    50101 caaataaagc ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt
    50161 atagtagcag caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc
    50221 aagtatccac catacttggt aactgttatt caaataattt tgggttcttt caataaatct
    50281 ctggtgagac agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg
    50341 cctttgccgc aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag
    50401 ccaatccatg aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac
    50461 cgaacagtta tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa
    50521 aatcactctt tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc
    50581 aaaacagcac ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt
    50641 tacgtctaag aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag
    50701 taataagaac agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac
    50761 cgcagatgca agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc
    50821 acagtctctt actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt
    50881 acgcgccatc agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc
    50941 agcaaactcc aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat
    51001 ccagaagtga acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga
    51061 ctctagttcg acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt
    51121 tgcttgtctt tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc
    51181 aataatctgc tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga
    51241 ttgagaaatt tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt
    51301 tggcaaaact tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc
    51361 ttccaaaata ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa
    51421 tgaaacgccc cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt
    51481 aatatgttcc atttccattt tgctacacct cactttgcca caatgtttaa tatagattca
    51541 ttgtagcgaa atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata
    51601 aaaccagcgg atttttcaac ctttcaagct atcttattta actataacat ttttggcaga
    51661 cgaatactat atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc
    51721 atcttctact acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact
    51781 tggaaaaact atctttgtaa tagt
//
LOCUS       DACCDF010000012        49860 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCDF010000012 DACCDF010000000
VERSION     DACCDF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.012"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(524..751)
                     /locus_tag="IVK51_000661"
     CDS             complement(524..751)
                     /locus_tag="IVK51_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430278.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="IVK51_000662"
     CDS             complement(760..1233)
                     /locus_tag="IVK51_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP6430279.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="IVK51_000663"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="IVK51_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6430280.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="IVK51_000664"
     CDS             3042..3230
                     /locus_tag="IVK51_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6430281.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="IVK51_000665"
     CDS             3243..3800
                     /locus_tag="IVK51_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6430282.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="IVK51_000666"
     CDS             3832..4074
                     /locus_tag="IVK51_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6430283.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="IVK51_000667"
     CDS             4415..5629
                     /locus_tag="IVK51_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP6430284.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..7087
                     /locus_tag="IVK51_000668"
     CDS             5660..7087
                     /locus_tag="IVK51_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6430285.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            7110..8036
                     /gene="glsA"
                     /locus_tag="IVK51_000669"
     CDS             7110..8036
                     /gene="glsA"
                     /locus_tag="IVK51_000669"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP6430286.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            8244..8699
                     /locus_tag="IVK51_000670"
     CDS             8244..8699
                     /locus_tag="IVK51_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430287.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKIKKSLSQNLTRGTSC"
     gene            8771..9703
                     /locus_tag="IVK51_000671"
     CDS             8771..9703
                     /locus_tag="IVK51_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430288.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9924..11687
                     /locus_tag="IVK51_000672"
     CDS             9924..11687
                     /locus_tag="IVK51_000672"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP6430289.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11771..12229
                     /locus_tag="IVK51_000673"
     CDS             11771..12229
                     /locus_tag="IVK51_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP6430290.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            12338..13021
                     /locus_tag="IVK51_000674"
     CDS             12338..13021
                     /locus_tag="IVK51_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6430291.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            13168..13782
                     /locus_tag="IVK51_000675"
     CDS             13168..13782
                     /locus_tag="IVK51_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP6430292.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13798..14004
                     /locus_tag="IVK51_000676"
     CDS             13798..14004
                     /locus_tag="IVK51_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430293.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14545..15675
                     /locus_tag="IVK51_000677"
     CDS             14545..15675
                     /locus_tag="IVK51_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6430294.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15767..16522
                     /locus_tag="IVK51_000678"
     CDS             15767..16522
                     /locus_tag="IVK51_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6430295.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16580..18619
                     /locus_tag="IVK51_000679"
     CDS             16580..18619
                     /locus_tag="IVK51_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP6430296.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18728..19879)
                     /locus_tag="IVK51_000680"
     CDS             complement(18728..19879)
                     /locus_tag="IVK51_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6430297.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(20033..20563)
                     /locus_tag="IVK51_000681"
     CDS             complement(20033..20563)
                     /locus_tag="IVK51_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430298.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20662..21012)
                     /locus_tag="IVK51_000682"
     CDS             complement(20662..21012)
                     /locus_tag="IVK51_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP6430299.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(21169..22698)
                     /gene="ahpF"
                     /locus_tag="IVK51_000683"
     CDS             complement(21169..22698)
                     /gene="ahpF"
                     /locus_tag="IVK51_000683"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP6430300.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMVMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22708..23271)
                     /gene="ahpC"
                     /locus_tag="IVK51_000684"
     CDS             complement(22708..23271)
                     /gene="ahpC"
                     /locus_tag="IVK51_000684"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP6430301.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23415..24077)
                     /locus_tag="IVK51_000685"
     CDS             complement(23415..24077)
                     /locus_tag="IVK51_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6430302.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            24261..25907
                     /locus_tag="IVK51_000686"
     CDS             24261..25907
                     /locus_tag="IVK51_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP6430303.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25898..26980
                     /gene="uxuA"
                     /locus_tag="IVK51_000687"
     CDS             25898..26980
                     /gene="uxuA"
                     /locus_tag="IVK51_000687"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6430304.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(27088..28641)
                     /locus_tag="IVK51_000688"
                     /pseudo
     CDS             complement(27088..28641)
                     /locus_tag="IVK51_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28789..29930
                     /locus_tag="IVK51_000689"
                     /pseudo
     CDS             28789..29930
                     /locus_tag="IVK51_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(30056..31135)
                     /locus_tag="IVK51_000690"
     CDS             complement(30056..31135)
                     /locus_tag="IVK51_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6430305.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(31238..31678)
                     /locus_tag="IVK51_000691"
     CDS             complement(31238..31678)
                     /locus_tag="IVK51_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6430306.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31735..32145)
                     /locus_tag="IVK51_000692"
     CDS             complement(31735..32145)
                     /locus_tag="IVK51_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6430307.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(32325..32759)
                     /locus_tag="IVK51_000693"
     CDS             complement(32325..32759)
                     /locus_tag="IVK51_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6430308.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32859..33707)
                     /locus_tag="IVK51_000694"
     CDS             complement(32859..33707)
                     /locus_tag="IVK51_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6430309.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            34096..35862
                     /locus_tag="IVK51_000695"
     CDS             34096..35862
                     /locus_tag="IVK51_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP6430310.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35964..37223
                     /locus_tag="IVK51_000696"
     CDS             35964..37223
                     /locus_tag="IVK51_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6430311.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            37370..38671
                     /locus_tag="IVK51_000697"
     CDS             37370..38671
                     /locus_tag="IVK51_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430312.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38674..39525
                     /locus_tag="IVK51_000698"
     CDS             38674..39525
                     /locus_tag="IVK51_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430313.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            39538..40353
                     /locus_tag="IVK51_000699"
     CDS             39538..40353
                     /locus_tag="IVK51_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP6430314.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            40504..41670
                     /locus_tag="IVK51_000700"
     CDS             40504..41670
                     /locus_tag="IVK51_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6430315.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41900..42547
                     /locus_tag="IVK51_000701"
     CDS             41900..42547
                     /locus_tag="IVK51_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP6430316.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            42631..43575
                     /locus_tag="IVK51_000702"
     CDS             42631..43575
                     /locus_tag="IVK51_000702"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP6430317.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43710..45161
                     /locus_tag="IVK51_000703"
     CDS             43710..45161
                     /locus_tag="IVK51_000703"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP6430318.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            45182..46018
                     /locus_tag="IVK51_000704"
     CDS             45182..46018
                     /locus_tag="IVK51_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6430319.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            46018..46677
                     /locus_tag="IVK51_000705"
     CDS             46018..46677
                     /locus_tag="IVK51_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6430320.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46803..47492
                     /locus_tag="IVK51_000706"
     CDS             46803..47492
                     /locus_tag="IVK51_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430321.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            47498..48454
                     /locus_tag="IVK51_000707"
     CDS             47498..48454
                     /locus_tag="IVK51_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP6430322.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            48498..49010
                     /locus_tag="IVK51_000708"
     CDS             48498..49010
                     /locus_tag="IVK51_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430323.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLAIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(49266..49814)
                     /locus_tag="IVK51_000709"
     CDS             complement(49266..49814)
                     /locus_tag="IVK51_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6430324.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
BASE COUNT        16749 a         8715 c         9654 g        14742 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcgt ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggcgg
     5881 tgtctatact tgggtcagtc gcacattggg aaaacgattc ggttttgccg caatcttttt
     5941 ccaatggttt gagataacag ttggctactt gaccatgctg tatttcttaa ctggtgcatt
     6001 atcctatgca acgggtattg gtgctataca aaacaataaa tatctgaaat tggcaatcct
     6061 attgattatt ttctgggcta ttttactctc acagttaaaa ggaaccaaat acacttcatt
     6121 gattgctcgg atcggttttg tagctggtat cttgttgcca gcattagtct tgtttggctt
     6181 agggatccat tatgtcgctt caggcgctcc actacaaacg atactgtcta tgaaaacatt
     6241 gattcctgat ttttcgaaat tgcctacgct ggttgttttt gtttccttta ttttagcata
     6301 tatgggcgta gaaacatctg ccagtcatgc aaatgaaatg gaaaacccta aaaaggatta
     6361 tccattagca atgtttgtat tggttattct ggcaatcatt ttagatactt ttggcggatt
     6421 gaccgtcgct actacagtac ctcaacatgg tttgtctctt aatacagggg tgattcaatc
     6481 gttagaattc ctattaagac acattgatcc aaatcttgca tggattgcga aaattcttgc
     6541 gattcttgtt tgtttaggtg tcatcggaga aattgcttct tgggtaacct ctccttcaaa
     6601 agcacttcac gtagcagcag aagaaggatt acttcctgaa tattttgcaa aagaaaacac
     6661 acacggtgtt cctgctcatt tgatgatcgc aaatgggatc gttgcaacaa tctgggcagc
     6721 cgtctttaca ttaagcggcg gggggaataa tctttcattt cttgcagcga tgtcattgac
     6781 tgttgtaatc tacctgatgt tgtactttat gtttttcgcg ggctatttga agctggtttt
     6841 taaagatgac agtcttgaaa gagcctatca ggttccaggc ggaaaagttg taaaagtaat
     6901 cgttgcttgt ttaggtcttt tgacttcact ttttgctttt tgtatctcat ttgttccacc
     6961 tgctagtatc gcagctggac agcatgggat ttatgaaacg attctgattg tttcttatct
     7021 tatcgtctta gttattccat ttacgatcta cgcatgccgt cataaattcc agcatgcaga
     7081 aaaataaaaa atgaaagctg gttattcaaa tggaagtaaa agaattagaa caattgattg
     7141 gaaaaaattt ccactattat caaacaggac aagtggcgga ttacattcca gcattagctg
     7201 aagtggatcc caaacaatta ggtatggcga tttatgattt ggatacaaaa caactgattg
     7261 agggaggaga cgccgatgtg cgctttgcca tcgaaagcat gtcaaaagtt cccgtattgt
     7321 tattagcaat caaagacaac ggaatagaaa aagtctttca aacaatcaac acggaaccaa
     7381 ccggttttgc cttcaattct atcatgaata tggaaatcaa tcatcgcaaa catccaatga
     7441 atccttttgt taatgcagga gcgattgcaa caacctcgtt gattgccggt aaagatgcag
     7501 atgaaaaatt tgagcgaatc ttgtcattta tgaaagagat ctgtgatgat cctaatatca
     7561 cattgaatga agaaatctat cattctgaat caagaacagg tgatatcaat cgctctcttg
     7621 cttactatat gaaaggtaat caaatgatcg aaggagacgt accggagata ctcgatgctt
     7681 atttcagaca atgctccctc aatgtcaccg ctacaggcat agcaaaatta gctgctgtgc
     7741 tggcaaataa aggaattgct ccttggaatg gcaaacaaat cattactgaa gaaagtgcta
     7801 caatcgtcaa atcaattatg acaactgctg gtttgtatga tgaatcagga gagttttccg
     7861 tccatgttgg tgtacctgca aaaagcggtg taggcggcgg gttgatggca gctgtgccaa
     7921 atcgttacgg cataggtgtc ttcagcccgg cactagatcc atttggtaac agcgcagctg
     7981 gtatccaatt gttgaaagat gtagtcaaag aactagacgc tgacatcttt gaataaccat
     8041 tcttgaaaga ggctggggta aaagtccaaa cggctcaaag ctacctacaa tgaggctgaa
     8101 attgtgagct tgtttgactt ttgtcccagt ttcttttttt attggttttt gaaagggctt
     8161 ttctaaataa aaaggaaaca aaaatacgat ttacagatat gattaaataa aatcataaga
     8221 aaaactggag gcgtgtatca ggaatgctgg aaaattacat cgagcgaaat atatttagaa
     8281 aagtgtatct gtgtgaacaa ttatttgaat tccaagaaat agacatcgaa caaacagcta
     8341 tttctctaag ggtgactaca ccaacgatcc ttcacgatct agaaagtcta gccgagtgtc
     8401 tagaatattg tataaaagaa caagtaagag agaagcataa gtataaactc gtattcaaac
     8461 atggtattgc tctttctgag ctcacacagt ttctgtacgg tcaatcttat tttttaaagt
     8521 ttttaagtta ttattttaat ggtatattca cgagtacaga actggctgat cttgaattta
     8581 tttctttgag taaagtatat acaatcaaaa aaatcgtatt ggattttttt aaagaaaatg
     8641 attatttgaa aataaagaaa tcattatccc agaatttgac tcgcggaaca tcttgctagc
     8701 tttagtacgt tatgtcaatt gggaaggcta tgaaaataaa aatccgtatg tagaagaagc
     8761 ttgtaacgag ttgattgatt atgtagagac tcattttttt aaaagaagat attcggaaga
     8821 tgaaaagtcc tttatcatac gagaaataga gatcgcaatc ggaagaaagg catttcccct
     8881 tggactgaaa gaagaagata agaaaagcgc agaaacgaag ccgttatttc aattagtctg
     8941 tcaaggattg gaagagcaaa aaggaattct agacttacaa gaagaagatg tctattatat
     9001 tttttctttg ttcaacagta gaaattatac caatgagaac atggagctgt taagaaaaga
     9061 tttcgaagtt gtttataaaa attttgttct caatgaccta tcactgaatg aattgatttt
     9121 acagattatt agtatcgctg aaatcgacga tgcagaaaat tttattttca agcaatcctt
     9181 tttacaattt gtccgtacgt tgtgggcaga tggacaagta tttttaccgg aaaggatcta
     9241 tcttctatca gaaaaagaac aacttctgta cggaaaaatt gtagaaatat tggaagagtg
     9301 gaaaaagaaa aatcaaatcc aactaagatg gaatgaaaat ttcattcgaa aattcgtcaa
     9361 aagtctttct ttgatcaaca gtgaacaagc ggggagacag gaaatagaaa tttttgttgt
     9421 ttcagaaagt tcagtcaaac aattttttta tcgaacccaa tttcatttgt atgcggaaga
     9481 aggcatcgct gaaataaatc ctattttatt tcgttcatta gaagaattgc ctgatgaatg
     9541 tctatgtgcg aaaaaaagag tggttttgtg cgatgctgct agctatcaaa atggattaga
     9601 aactgaaaag acaaagatct atccaatctc attaaaaggt gcaaacatcc atttgtatca
     9661 tattgctcag gaacttcacc tattaaaaaa acatatttct tgatgagacc ttaattagga
     9721 gaccttctac aaagcttttt aattgtctgt agaaggtttt tatttgagtc ctgattcgtg
     9781 cgaagatcta cttatataac aagctttctc tgaaattttt ttaaaatgct attgacttag
     9841 agtcaactct aagtagtaga atagataaag tgtaacaaat ctattttcat taaaagaaga
     9901 tagtacggag gagagaatgt attatgagta cagtattaca tgtattggat gcaatcaata
     9961 gccccgcaga tgtcaaaaaa ttatctgttg aggaaatgaa acagttggca gaagagatgc
    10021 gccaaaaaat cttgcaaaag gacagcgatg taggtgggca cgttggtccg aatctaggtg
    10081 tagtagaatt aacgattgcg tttcattatg tatttgattc accaaaggat aaaatcgttt
    10141 gggatgtttc ccatcagtct tatggtcata agcttttaac tggtcgaaaa aatggttttg
    10201 aaaaaggaca ttaccgtgaa gtgagcggat attccaacca acatgaaagt aaacatgact
    10261 attttacagt ggggcacaca tctacatcta tcagcttagc aacaggttta gctaaagcga
    10321 gagatctgaa gaaagaatca ggaaatgtgg tagcatttat cggtgacggc tccctaagtg
    10381 gtggcttagc ttttgaagga ttgaacaatg gtggtgtttt acaatccaat ctaatcgtat
    10441 tagtaaacga taatgagatg tctattgatg agaatcatgg cagtttatat aaaaacttag
    10501 ctgagcttcg tcaaaccaac ggtaaaagca gcaataacct atttaaagca atgggatttg
    10561 attatcgtta cgtggataat ggaaacgatc tggaaacggt gatccgttta tttgaagaag
    10621 taaaagatat tgatcatccg attgtacttc acgtccatac ggaaaaaggt cgcggttatc
    10681 agccggcaat cgataataaa atgaaatatc attggcatgt accatttgat ctgtccaccg
    10741 gggaggcaaa gcaagtaact tctggagaaa catttaatat gatcatcatg gattatttgg
    10801 ataagaaaat gcaaacaagt acagctccta ttgccgcagt caatgcagcg ataccaggcg
    10861 cctttggact gaaaacattc caagaaaaac atccagatca ttattttgac gtggggatcg
    10921 ctgaacagca ctctatcact ttcgcttctg ctttggcttc tcagggagta cgtccagtcg
    10981 tcttccaatc aagtactttc ttgcaacggg catatgatca attatcacat gatttagcga
    11041 tcaacgagaa tccagtgatt attttagtcg gtggcggaac gatctcagct agtgaccgaa
    11101 cacaccaagg aacatttgac attccttact tatcaagtat cccgaacatc aaatatcttg
    11161 ctccagcaac aaaagaggat atgatctcta tgctagattg ggcgttcgaa caaacagcgc
    11221 agcctgtagc aatccgaatt cctacacatg gtgtagaaca tggtacagct atcgcaaatg
    11281 attatagtat tccagcctat caaacagtaa ataatggagg aaaagtagca attttagctt
    11341 taggcggttt tcttgaatta ggaaaagaaa cagtaaaagc ttttagaaaa aaaggaatcg
    11401 aagcaacgtt gatcaatcca ggaacgatca cagaaaatga cgtagaaaca ttagataaat
    11461 taaaagaaac acacgaatta gtcatcacat tagaagatgg tagtttgtca ggaggatttg
    11521 gcgaaaatat cagtcgttat tatggtgatt cttcaatgaa cgtattaaat tttggtgcag
    11581 agaaagaatt cacggatagc gttcctgtag ggcagcttta tgaacgttat catttaacac
    11641 caaaacaaat cgttgaagat agtctgcgtg tcttgaatat tcaataattc attatgctca
    11701 ccatccagat aattgtttat actagtatct ggatggtttt ttcattataa aaagcaaagg
    11761 ggtggatgtt atgtatcagc aagtagaaga atatgtcaaa aagttccaag aaaaatatcc
    11821 tagttatcaa tcagaaaaat taggtttctt ttcatttggg gatacagaaa aaatggcaga
    11881 cgagttgagc gaactagttg taaaaaagat aaagactggt acatcaagtg gttacgatct
    11941 gtataaacaa gagaatgagc ctttaccaaa agttggtcaa ttagacgtgg tcctgaatgg
    12001 aaaaaatgaa ccagtttgta tcatcaaaaa tataaatgtg accatcttgc cttttagtga
    12061 agtcacgaaa gagcatgctt ggaaagaagg agaaggggat cggacattag cttattggcg
    12121 gaaagtccat gctgagtttt ttaaacatgc ttataaagaa gcgttgaaca tagaatttac
    12181 agaagagaag ctggtcgtat acgaagaatt tgaagtaatt ttcttttagt aagaaaggtc
    12241 tgctgaaaga aatagctcat tattgtctcc tgaatagaaa agggttacat ttttatatga
    12301 agctgtattt ttatataaca ttactggagg agatattgtg gatacactgg aacaaatcaa
    12361 aggcggagtc gtcgtttctt gtcaagcttt ggaaaacgag ccgctacaca gttcttttat
    12421 tatggggaga atggctttgg ctgcaaaaga aggcggagca gttggtattc gagcaaatac
    12481 gacagaagat atcaatgaaa tcaagcgaat cgttgattta ccggtaatag ggattatcaa
    12541 aagagattac gatgggtcag aagtgtttat tactgcgaca gaaaaagaag tggaagaact
    12601 tttggagacg caagcagaaa tcattgcact ggatgcaact tctcgtaaac gtccgacaga
    12661 aaaaacaact gctgaacttg tccaaatgat tcacgaacac ggtcggttag ctatggcaga
    12721 tatttcgact tttgaggaag cgcttcaagc gcaagaagat ggttttgatc ttttgtctac
    12781 gactttagca ggctatacag aatattcacg aaagacgcag acacctgatt ttgatttatt
    12841 gaatcaaatc atagaacaag tcgatatccc agtaattatg gaaggacata ctgactctcc
    12901 tgaacaagta gagaaagcat atgaattagg tgctttttca gtcgtagttg gatctattat
    12961 cacccgtccg caattgatca ctaaaagata tgtcgaagcc gcaagtaaag caaataacta
    13021 aaaaacacgc acggcacaga ttcttgacaa aaaagaaaaa ttgatagata gtttaaagca
    13081 gattctaaga agatgaaaaa agcattactt ttcattttgt cttcaagatc tgctttttaa
    13141 cgaattttat agaaagaagg cgaaaccatg tttgaacgga gtccttcaga attgacagaa
    13201 cgtgaaaatt acaagttgct gattggttca gtcatcccta gaccagtagc agtagtaacg
    13261 actaaatcag caaaagatat agtgaatatc gcacccttca gttacttcaa tatcgtcagt
    13321 tcgaatcgac ccatcttatc cttggcgatc cagagaaaac aagatgcagt aaaagacaca
    13381 gcaaagaata ttcttgagac aagagaagct gttatccata tccttgatga ggacaacgta
    13441 gaagaagtaa ataaaacagc agccaatctg cctaatgagg aaagcgaact gtacctgact
    13501 gacttgactt taactgattc agtaatcatt tcagtaccag cattgacaga agcaaaagtt
    13561 cgttttgaaa catccttata tgaacatgtc gagatacgga ataaaaataa agtaacagct
    13621 gatttatttt tgctagaagt acagaaatat catttaaatg aaacagtcta tgatgataag
    13681 acaggaagga tcgatccaag aaaattgaat gctgtaagcc gtttggcagg aaatgattat
    13741 gcagcaatag gagaaatttt cacgattgca cgtcccgaat aaaaaggagc actagttatg
    13801 ttaaaaataa gctataaacc atcgacagat tcaaaagaaa tgaaaaaaga atatgaaaca
    13861 gtgaatgatt ttctgcaagg gcagtactta gaagttccgc cattgcaaga ccattttgtc
    13921 gttactactg ttactttaga tggtaaggaa atagaaatgc cagatcaaac gattagcgga
    13981 ttgtttaatt actttaacaa ataaatattt attccgaaaa gtgaaaaaac atataaaaaa
    14041 acacataaaa ctaaaaaatt atcaaagaat cctggtgcaa ctgaaagaat ttgcaacggg
    14101 attctttcaa cttatttgtt atttttttct aaaaaatatt aaagattgtt aaatttcact
    14161 atttcatttc aaattccatt gcctaatcat taggaaaagt ctatcatgaa ctcgtaaata
    14221 gaatatagta ataaccttgt taggtgaggc tcctatacaa acataggctg ttgcccagaa
    14281 acgtcgaaag tcgccaatgg gtaaaacaga aactgttgga ttaaggcttt ttccaatagc
    14341 taagaatgtt ttctttacgt tgtatagtgc taaaactcgg acgatgagat gatgctaggt
    14401 cagaattctt gttcgaaaag gtttctgacc ttttctttat atttttgtga ctgacttatt
    14461 tgatgagaca aactcgctcc tcacctgaca agtggattgc tgtgtgagga ggagatgcgg
    14521 tgaaaaaaga tcgattggtg gattttgggt ccttatttga ctttgtccat gatcggtctt
    14581 ttagaagtat acagtgccag ttcttatcgt ttattgcaag ccgatgaaaa tacaaaaagt
    14641 ctattattgc gtcaactaat tttcatattt ttgagttgga gcgtgatctt cttagctcgt
    14701 tcagtcaaac tacactattt acttcaccct aaaatagcag gatacggttt agccttatcg
    14761 attttctttt tagtattagt aagaataggg atattcggtg tcactgtcaa cggcgcacaa
    14821 cgttggatct ctctgtttgg cattcaattc cagccttctg aactggcaaa tctttttttg
    14881 attttttatt taagctggtt ttttcgtgac ggaaatagta gcccaaaaga tctaaaaaaa
    14941 ccattcctga ttacagtagg tataactttt ctgattttat ttcagccaaa gattgctgga
    15001 gcattgatga tcctttcgat tgcgtgggtc atattttggg cagcggcggt tccatttaaa
    15061 aaagggatct atctaatcgt tactttttct gcattgctga ttggagcagc aggcggggta
    15121 ttatatttag gaaataaagg ttggcttcca cagatgttta atcatgctta tgaaaggatc
    15181 gcgactttaa gagattcttt tatcgatagt catggtgcgg gctatcaaat gacccattcg
    15241 ttttatgcac tatataatgg tggcatattt gggcggggat taggaaatag tatcacaaaa
    15301 aaagggtatc ttccagagac agaaacagat tttatttttt cgattattac agaagagctt
    15361 gggctaatcg gtgcattgtg tgtgcttttc ttattgttct cattatgcat gagaatcttt
    15421 tgtttaagca gtcgctgtaa aaatcagcaa gctggattgt ttcttttagg attcggaact
    15481 ttgctattcg ttcagacgat aatgaacgtg ggaagtatcg cggggctgat gccaatgact
    15541 ggtgtgccat tgccatttgt tagctacgga ggaacaagtt atctgatcct ttctttagga
    15601 atcggaatca cgttgaatat ttcttcaaag atacaggccg aagagttggc gctctatcga
    15661 cctgaaaaac aataaagaga aactgagaaa ctctcagttt gaattatcat aaagtacaat
    15721 cttttatttt tcaagcgaga ggttctttta gaaagaggaa ttcgcaatga aagtatctca
    15781 aaaaatcata ttatctattt tgttactgct tacattagta gttggctatt tttcgattga
    15841 gtttatccat ggattttcgt ctgcaaaaca gagttcaact gtgaaaaagg tagagccgaa
    15901 aagtatccct accacactaa atgtggcttt gattggttca gatgctcgtt cgaaagacga
    15961 aaacggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac aacaagcaaa
    16021 actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa tgaataagat
    16081 aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa aggaaaattt
    16141 caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg attgcgttaa
    16201 tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt tagatggcgt
    16261 atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt atgctcgatt
    16321 tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag tgatcaaagc
    16381 tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag cggttgggaa
    16441 attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt gtggtatgga
    16501 tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa
    16561 gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc
    16621 ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca
    16681 gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca
    16741 agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa
    16801 taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc
    16861 tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta
    16921 cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta
    16981 taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt
    17041 ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg
    17101 gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg
    17161 agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc
    17221 ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca
    17281 acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg
    17341 agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct
    17401 gattggttac gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag
    17461 taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac
    17521 aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga
    17581 acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac
    17641 agcttttgac agtctaggag gaaaagctgg atcaactgtt gcgacaacgc caaaaaccgg
    17701 tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat
    17761 ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt
    17821 tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga
    17881 caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga
    17941 cagttcttgg ggatcttatc aagcaacgcg tgtcagtagt gatgtatcac aagtagactt
    18001 aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg
    18061 tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc
    18121 aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct
    18181 agctgatact ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat
    18241 gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga
    18301 gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct
    18361 gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc
    18421 gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga
    18481 gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt
    18541 ggaaaataaa gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct
    18601 caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat
    18661 cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt
    18721 gatttactca cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa
    18781 gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac
    18841 tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac
    18901 cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa
    18961 gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa
    19021 agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat
    19081 atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta
    19141 tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca
    19201 ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac actttttctg
    19261 caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa
    19321 ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa
    19381 aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca
    19441 aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa
    19501 tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt
    19561 ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa
    19621 cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta
    19681 aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga
    19741 cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg
    19801 ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata
    19861 aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca
    19921 taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag
    19981 atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta
    20041 tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa
    20101 taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga
    20161 atccgccaat caacaacaga atactactat aaacactcca acttttagct tttgtattca
    20221 tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga agaatcggca
    20281 ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa
    20341 gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa
    20401 aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa
    20461 aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta
    20521 ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt
    20581 gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg
    20641 agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc
    20701 tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc
    20761 ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg
    20821 ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt
    20881 ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt
    20941 tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg
    21001 attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat
    21061 aagaaaaaga gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct
    21121 tgtcaccatc cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat
    21181 cagataatcg aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg
    21241 tttgtatgga ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc
    21301 atttttctcg gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc
    21361 cgtattaggg agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatcac
    21421 catcgtacgg cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt
    21481 cgtcgctgca tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat
    21541 ttgatctgct tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc
    21601 caaatcgatt gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc
    21661 tttaaataaa gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt
    21721 ttcgcctggt acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc
    21781 tttaagtgat ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga
    21841 aacggcctgt tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg
    21901 ctgcatcaat tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt
    21961 ttcaacgact tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc
    22021 tgcatagata gctgcactac tagctgctgg tccaccgcca atcactaata catcataagg
    22081 ttctttttca aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc
    22141 gatcgtcatg cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat
    22201 gattttttta tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat
    22261 atttggattt aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca
    22321 agtcaagcta acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact
    22381 caaagattca ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga
    22441 ggaaaattca tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg
    22501 attgatagaa aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga
    22561 catttctgct atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa
    22621 actaacagaa aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt
    22681 agtttctttg tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt
    22741 caatgtttcg ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac
    22801 atattgagca gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc
    22861 attgatttcc atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc
    22921 taatccttgc tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc
    22981 gatcatcacg tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt
    23041 aaagtgggta tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta
    23101 ttgttcttgt aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa
    23161 gcaaagaata ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg
    23221 atgataagca tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc
    23281 tccaataata attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg
    23341 atattttgtt atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag
    23401 gtcgcaagaa aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt
    23461 tctttcagca tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta
    23521 tcaactaaag ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc
    23581 tgaatggtca ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag
    23641 ttccaaactt cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca
    23701 cggaaaaaag cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt
    23761 tctagcacta ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct
    23821 tccaactgtt ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca
    23881 tacgtaccac tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct
    23941 tcgcgaatgg gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc
    24001 cttccaggtt ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat
    24061 tgcaagctgc ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga
    24121 agaggtctga aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa
    24181 attttggttg acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat
    24241 gttagtttag gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat
    24301 tatctcaaaa agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac
    24361 aaaaaaatcc tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag
    24421 aaatcgctca acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa
    24481 cattcgatga agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag
    24541 tggtcatgcc aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc
    24601 tgtattgtca tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc
    24661 ctagtttaca attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa
    24721 ataataaata tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac
    24781 atacaatgag tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga
    24841 ttgcacttgt ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc
    24901 taacgatcgc aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact
    24961 tatcgaatga agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc
    25021 cttccttgtc agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc
    25081 aaaccgcaaa aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa
    25141 tcatggaaga tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga
    25201 ctgatcgtgc aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt
    25261 tgcatacgtc tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga
    25321 tgaaagatcc agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag
    25381 ttgtggagtc tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc
    25441 gcttttctaa tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa
    25501 aggtggcgat tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc
    25561 cgcagacact tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca
    25621 ttgatgatca aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc
    25681 ataagctttc cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg
    25741 ctcctatttt gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca
    25801 gaaaaataga agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat
    25861 tacaggatta cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta
    25921 cggatcacag gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg
    25981 gattgtaggc acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa
    26041 agaactaaaa gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa
    26101 tgtgcatgat gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa
    26161 gcagacaatc cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc
    26221 tgtatttgac tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt
    26281 agcttttata tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca
    26341 ttccagcaat gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc
    26401 cttgcttgaa gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt
    26461 tctccaatca atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga
    26521 tgatccgcca tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga
    26581 ttggttgtgt cgcatagtag acagccctta taacggaatc actctttgta cagggagtat
    26641 tgcagaagat ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg
    26701 aatcccattt gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc
    26761 accacataaa tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga
    26821 caacggattt aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg
    26881 aagaccagga tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg
    26941 ggaagcaatc gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta
    27001 ttatgtaaat aagaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag
    27061 cattcggatt tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg
    27121 atccatgagt gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt
    27181 tttccagtct tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg
    27241 caattgtttc aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg
    27301 tattttgatc gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat
    27361 ataaaaatca cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc
    27421 atacaaatgt actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg
    27481 taacaagtgt tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac
    27541 ttctttcaat acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg
    27601 attgaagtta agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat
    27661 cttttctttt tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct
    27721 tgaataaaga acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt
    27781 ttcttccgat tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg
    27841 ttcgcgacaa attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc
    27901 aatatattca tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt
    27961 ctctttacat atttttaata gaaaatataa tttatctatc ataccgctat ttagtcggtc
    28021 aagtacttct tttttaaaaa aagtattcat cataaaagcc actacttgcg gatcttcatc
    28081 ataaaattct gtagcagtac ttgaaaagat gttccaataa atatctaatg ctgcatattg
    28141 aatttgtacc tcttttccag aaaaagactt tctgccatta ttagaaaagg agatagacaa
    28201 accgtactat gctaaaaatg cattaatctt tttcattttc gtatacaaat aagaggcact
    28261 atagtttgtt tgctgactga aactgataat cgaatttgtc tgacagctga acaaagaagt
    28321 caagatctga taactgactg attcgttgca gtatcttcca aatagacgat aatagcagta
    28381 agccggatca attccttcaa aattcaactt taaaaaaggt ttttgcttat caatatgcag
    28441 ttctgttaga ttgagacgct caaagtcttg ttctaattct ttgatgtata gtaaaaaatt
    28501 tgattcagac atatggaaat gatctgctaa atattgtgta gtaataaaga aacgatgttc
    28561 agataggaaa agaaaaatat ctaattttgt attgcttttt ttcttcaaga taaacgtttt
    28621 aatcgcttct tgagtttgca tgaaagaggc tcctttttac ttaaattgtt aaaacttgaa
    28681 aatattactg tttttttcat aaatttagac aaaaaaacag taaacatata ggtagaatct
    28741 ctactatctt atttcttgca tacaaaaaaa tcaaggagtg agagagttat gagatattca
    28801 accatcttaa tatcaacatt tgcagcctta agcttatcgc ttactataaa tcagacagtc
    28861 cacacagagg agacagaaac agccaaatgg acagaaacca agtgggccaa cgtcaatcga
    28921 attacctttc aggatgaatt ttcatccttc aattcaggag tcgcaaaatt aagaaaagga
    28981 caaacagcga ccatcaaagc aaaaattgac tattcgggag acttagcacc tatactacaa
    29041 tcagctgtta catttgagaa tgttgatccc actttgtcga ttggctatga cgagaacact
    29101 ttgactatcc aacacaagca agcgatattt accttcaccg ttacattaaa tcaggacttg
    29161 ttaaaaccag cgctttttac tgtcaaggta tcagatggac taccgaacga tcatcattta
    29221 acgccgtata gtcagcggca gacaattgag caagattcag ccatcgactc aggaggagat
    29281 cttgttgaag aaccaacaga taaaccagaa aatgaaaata agccagaagt accgcctaca
    29341 gaaaatccag acggagaaca gaaaccggaa atagaacctg gagaagaacc cgatacagaa
    29401 actcaaccag agccagacaa taaatcaaaa cctgaaataa cccctggaga aaaaccagat
    29461 gtaactccag aaccagatac ggattctgga aaccaaacag taccggaaac aaatccagac
    29521 acagataatg aaactgaaaa tccagaaaaa ccagaggtag atccagaaga aaaaccagat
    29581 gtgactccag aaccagatac ggacgcaaga gaccaaggaa taccagaaaa aataaacaaa
    29641 aaaacaattc aagaggatgg aaaaaaagag agtaaaaaat caaatctagc aattctaaaa
    29701 ataaatgaag agcagctgaa taaaaaatca cgaatatttg atagcgccca atcagctgaa
    29761 accttaaaaa gtagcaagga tacaaccttt gctgtcctga aacgaaaaat aaacagctac
    29821 caaaatccgg agagtcacaa aacaaagtta tactgtggag cggaatcatt ctattaagta
    29881 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
    29941 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaatgacc ccaaaatacc
    30001 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
    30061 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
    30121 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
    30181 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
    30241 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
    30301 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
    30361 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
    30421 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt atttaaaaga gcgacaacaa
    30481 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
    30541 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
    30601 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
    30661 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
    30721 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
    30781 gatgctcgta aaaattaata acggactgga tcatgtcaaa tgtttgatga ataagcatcg
    30841 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
    30901 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
    30961 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
    31021 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
    31081 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
    31141 gatggaatat gaaaataaga aagagcaaaa agaaaaaccg ctaaatgacg ttatcttatg
    31201 agttggtttt cctcataaaa tcgttcatct agcagtttca gccctttttc tggtcttgat
    31261 gaaaattatc tagcattttt cgggtcagat gttgcagtgt atccaattct tcctgttcaa
    31321 gtggaagata agacttgatc ttttgtggaa taccttctgc ttgtttttgc aacgcagcac
    31381 cttgagcagt cagcgtaaca tttacgattc tttcatcacc cgtttcacgc tgacgattaa
    31441 tataattttt ttctgctaac ttttttagta agggagtcag cgtaccagaa tccagacaca
    31501 aatcatttcc tagctctttc actgaagtct ttttcttttc ccataaaact aacatcacaa
    31561 gatactgggt ataggtaaga ttcagcggct tgaggagggg agtgtagtgt ctaatgattt
    31621 ctttagaggc ggcatataaa ggaaaacata cttgattaac taaatataga tcttccattt
    31681 ttccacctcc gctgttttac tatagtgtat catagcggat ttacacaaaa aagtctattc
    31741 actaactgct ttcagagtaa cttctatatt tccagctgta gcttttgaat acggacacac
    31801 tttatgcgct gcctctaata attcttgcgt ttcttccaga gaaagacctt cgatagatcc
    31861 ttcaatctct acacctaaga caaagctcgt ttcactttct gaataaaggg aaacttgtgc
    31921 actgacagta gaagccccag agatattttt ttgctgtttg atcaattcta atgcactgtt
    31981 aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg ttggttgcat tttcgacgcg
    32041 tttaccagga gctgcaatat tcaattcgaa tgtacgatcg ggattatgca cttctccaga
    32101 gcgtccgcct gtgttgatca ttttcgtaga atacattttt ttcataaaat taccaccttt
    32161 aattatattt tatacaattg aattttacac aatataattg tggaaagcaa agaaatggat
    32221 tggcataaga taaataagag aaagcacatg aaacagacgc gctttctcga atagcaaaca
    32281 tatacaaaaa atatcagaga caaaaaataa tctcatcata aacattacat atatttctca
    32341 aaaagttgtt ttctttcttc aaaaattact tcacgatgga cctgatgaag catccataaa
    32401 taaccaaaag gatctctgaa cattgagtta gtcacaccaa agtccatcat ttctgtcatt
    32461 tcttgaatct ctacacagcc atttcgtaat gctttattat gtgtgtcctc gatatcagag
    32521 acgatgatat tgaaccagat cgtatttgga tgttcagcag taggagcagt caaaccaaga
    32581 gacgtgtttt catccagcat atggaatctc gtaccataca aggtaaaaat gacttcatta
    32641 ttgcctctag ggtaatctgt tgcctcaatc agttgtattt catcaaagat tgtttgatac
    32701 agttctagtg ctttcaaact atcatttaat acaaaatcga tttccgttcc tttatgcata
    32761 aagatctcct ctccttttca gtcgaacagt tactagattc ttttattata ccagctaaag
    32821 aaaacgcatt caaaaaaata gcggatttac ttaccaactc attcgattgg atgataagta
    32881 aatccgcgtc ccattacttc tgctacgctg ctgacaatga caaaggcact tggatcgatt
    32941 tcttctatca atggtttcat tcgggaaaat tcatgatcag tcacaacgat caataaaacc
    33001 tcttttgact ttcgattata cccgcctttt gcctctagca gggtaaggcc acgtccaatc
    33061 tcagcactta agcgaaggcg gatgtcttcc aaataatgtt cactcatgac cataacagat
    33121 ttctttcgat tgatacctgt ttcgatataa gtcatgacaa ttgaagtgat tgcaatagat
    33181 aaaatagcat aaagaaactc ttctattcca aaaataaaca gattaagaga aacaacgaat
    33241 gcgtctgtaa aaaataagcc aatcgaagta ttcaagtgga aatatttttt aaaaatgagg
    33301 ggagggatcg tcgtaccgcc gctagaagag ttgtttttat ataaaatcgc aactcctaaa
    33361 gcaaaaatcg cacttccaaa aatgacagac aacaaccgat ctgaagtaat cattgtttct
    33421 ggaactattg caatgattaa aggaaaaacc aagcttccga aaagtacttt aaaaaagggt
    33481 tttttaccta aaaatagaag agctagtacc aacataacga tattcaacac taaaataact
    33541 atagctcggt caaagcctaa agcagcttcc agtaatattc ctagaccgct gacacctcca
    33601 gcagcaatat gatgaggccc taaaaacata ttgatagcga ctcctaatat gaagagggca
    33661 aaaataatct taatgtatgt ccatataaat tgtctcgttt ctttcattta ttcacttcct
    33721 taattcatta ataatactct tatcttatta taacttactt ttcaccttaa gttaacttta
    33781 attaataaaa aaattaggct agtctaaatt attatgtttc attcttctag aatatacttc
    33841 tacgccaaaa actgttaaaa aacttttact ttagaaaatt ggagaaattg atcgcttaag
    33901 ataaattcag gacactaaat agtacaggaa gtagactaaa acttttaaaa gttttaagtc
    33961 tatctatttg gcaagtttaa aaaataaaaa ttcggtatca aaaaactgtt accgataaca
    34021 tttttgaaag cgttgtcttt tgcttctcta tcacttatag tgatgacgtc atattaaaac
    34081 acaaggagga aatcaatgga tacagctgca atttatcatc gaccggaaag tgaatatgca
    34141 tttctatata cgaatacaag atttcgtatt cggcttcgta ctaggaaaaa tgatatcaaa
    34201 aaagtatatg tgctatgtgg agatccatat accataacaa ctgaaaaatg gtatcaaaaa
    34261 caacgaccaa tgaaaaaatg tctaagtacc aatgttcatg attattggga aattgaagtg
    34321 acgagcgaaa caagtcgctt acagtatgcg ttccatgttg ttggggaaga taacacggac
    34381 tgtttttacg gtgatcaagg aatttttcct tatcgagaaa atgtgattac tgaaccaaat
    34441 ttttattttc gaatacctta ttttcatcaa atcgatcgat tcactattcc agaatgggta
    34501 aaagaaacag tatggtatca aatttttccc gaaagatttg caaacgggga caaagcaaat
    34561 gaccccgaac acactttacc ttggggaagt aaagatccag accgagaaga ctttttcggg
    34621 ggagatttgc aaggtgtgat ggatcatttg gattacttga cggaccttgg catcaatggc
    34681 atctattttt gtcccatatt taaagccaca tccaatcata aatacgatac gattgattat
    34741 tatgaggtgg atcccgcttt tggcgataaa aaacttttaa aaaacttgat tgatgaagca
    34801 cataaaagag ggatacgtat catgttcgat gctgtattca atcatatggg tgtccattca
    34861 ccacagtggc aagacgttct caaaaacgga gaaaaatcga tctataaaga ctggttccac
    34921 atccgttttt tcccagtaga ttcttatcag atgactgact taccagaaac tgctgaaaat
    34981 atcccatatg atacttttgc ttttactcca ttcatgccga aactaaatac agcaaatcca
    35041 gaagtgcaaa aatatttgct ggatatcgca acttattgga taaaagaatt cgatattgat
    35101 ggatggcgtc ttgatgttgc taatgaagtc gatcatcatt tttggaaaaa attcagagaa
    35161 gcagttacag aaatcaaacc agacatctat atccttggag agatttggca ttcctctcaa
    35221 gcttggctgc aaggggatga attccatgca gtgatgaatt atgcatttac agattcgatt
    35281 aaagattatt ttgcaaaaaa gaaaatcaca gcttcacaaa tggtcagtgg aatgaatcat
    35341 cagcaaatgc tttacagaga tcaggtgaac gaaggaacat tcaacttatt agattctcat
    35401 gatactgcca ggatcttgac cctttgccaa ggtaacaaag aattgatgaa gtctgtgatg
    35461 gcctttatgt ttcttcaaaa agggtctcct tgtatctatt acgggacaga aatagggatg
    35521 actggtgaag atgacccgga ttgccgcaaa tgtatgatat gggagaagga ggaacaagat
    35581 ctagagctat tcggttttat aaaagaactt gtttctctaa ggaaacagtt gtctaagatt
    35641 atttctgaag gttctactca gtggctaata gtaaatgatc gtgaagataa gctctatttc
    35701 acacggaaac tagagggaca gatcatctat gtttacttta atcagtcaaa agaaccttgg
    35761 gtagtagaac aggaaaatga ggtgattctt agtcaaaatt gtcagttgtt agaagacggg
    35821 aaagcagaga tacgacaata cggatttttg atcatgtgtt gaaaactgtt accggtaaca
    35881 acaagaaagc gtttgcaaat gaagatttgt gcccattata ataagggtgt aacagcacgt
    35941 tgaatacaaa ggaggaaatg aagatgaaaa aaagaagtgt gaaacagttg ggtttagggt
    36001 taatctcttt aggaatctta ggcggattag cggcttgtgg caatggtaac tcttcagcag
    36061 aaggggaagc cgatgaagga aaaacgttaa cagtttccgt tgattctgga tataaagatt
    36121 atataaacga aataaaaaac actttcgaaa aagaaaacga tgtgaaaatc aagctagtag
    36181 aaaaggacat gttcgatcag ttggaatcat tagcgttaga tgggcctgct ggaaagggac
    36241 ctgacgttat gatggcagca tatgaccgga tcggtgcgtt aggacagcaa ggacatctgg
    36301 cggaagtaaa actgggaaat gaagcagctt acgatgaaac agacaaagcc caagtgactt
    36361 atgatggaaa aatctacgga gaacctgcag taatcgagac ccttgttttg tactataaca
    36421 aagatttagt cgatacagca ccatcaacgt ttaaagattt ggagaatcta tccaaagaca
    36481 gtcgttttgc ttttgagtca gaagcaggaa aaaatacagg atttttagct aaatggactg
    36541 acttttatta ttcctatggt ttaattgcag gttacggtgg ctatgtattt ggtgatgatg
    36601 gcacaaatcc ttcagatatc ggtttgaata atgcaggtgc tgtagaagga atctcttatg
    36661 caacagattg gttcaaaaat gtttggccaa agggaatgca agacataaaa agtgcaggag
    36721 acttcgccag ccagcaattc atgtccaata aaactgcagc aatcattgac ggtccatggc
    36781 aagcgcaaac atacaaagaa aacaatatca attatggtgt agccaaaatt cctactctaa
    36841 ataatggaca gccttatcaa ccgtttggcg gaggaaaagg atgggtagtc agcaattatg
    36901 ctaaaaacaa agacctctct caaaaatggc ttgattatgt aacgaatcag gaaaatcaag
    36961 aaaaattctt tgaaatggta aatgaaatcc ctgcaaacca acaagctagg gaaacagcaa
    37021 aggctaaaaa cgatgaatta acaacagctg tgattgaaca atatgaaaca gctcaagcga
    37081 tgccaaacat cccagaaatg ggcgaagttt ggacaggtgc agaaaacttg atgttcgatg
    37141 cagcatcagg ttcaaaaaca ccaaaagaat ccgcagatga agcagtaaaa acaatcagtg
    37201 aagccataga acaaaaatac tgattaaaat gataaccgtc catttttata aaaaatggaa
    37261 caggtgcttc agataaaagt ggcaagagcg agatggaaaa ttatcttgct cttgttgcca
    37321 ttatcttaaa agcagtttgc ggtttagcaa gtttagaggg ggcattcaga tgcagcaaga
    37381 taaaacaagc aatatcaacg aaaaaaaaat cacgctttgt tcacttattc cagggatggg
    37441 gcagtttgta aatggccaag cttttaaagg tatcgttttt ttcgccatat tcgtcctatt
    37501 cattggtcag atgcttctgg gcggatttca tgcattggta ggattggtta ctttgggaag
    37561 tataccaatg gaagatcatt cattatttat tttgattgaa ggcactttgc aattcatcat
    37621 tacaatcatt ttcttgattt tttacggttt gaatctttta gacgcttatc gagtagcgaa
    37681 gatgcgtcaa gagggcaaaa aaatcaatcg gacgatgaag gaagtcatac ataatatagg
    37741 tgatgaaggt tttccttatc tattgacact tcccgcttat cttctcatga tttttacgat
    37801 cattttccca gtcctagtta cattgttcac tgcatttact aattatgact tcaatcatat
    37861 tccaccagcc agtttaattg attgggtagg ggctaaaaac ttttttagta tcttcttttt
    37921 aagctcatat cgtaatactt tttttgcagt cttttcgtgg accgtgattt ggactttatg
    37981 tgcaacaact ttgcaaatca cccttggtat tctcacagcg gttgttgctc accagaaatt
    38041 tattaaattc aaacgtattt tcggtatcat tttcttatta ccatgggcgg tcccagcttt
    38101 tattactatc atgagttttt cgaatatatt caacgatagc gtaggtgcca tcaatactca
    38161 agtgattcct ttcataaacc agcttccttt tgtagatatc agtgcaatct cttggaaaac
    38221 ggacccgaac tggacgaaag tagcaattat cttagtgcag ggctggttag gatttcccta
    38281 tatctatgtg atggttacag gaatcttaca atcgatttca gaagacttat atgaagcagc
    38341 aaaaatcgac ggagcaaatg catggcagcg cttttcaaac atcacgcttc ctgctgtctt
    38401 cctagtggca gcgccaacat ttattacaca gtatacgggg aatttcaata atttctccat
    38461 gatctatctg tttaacaatg gcggtcccgg aagcgtaggc ggaggagctg gttctactga
    38521 tattcttatt tcctggatct ataaacttac gactggaact tctccgcagt attctatggc
    38581 ttcagcaatc acgttgatta tctccgtcat cgttatctgt gtgtcattgc tggtatttaa
    38641 aaagacaaat gcgttcaata tgggggatta acaatgaaaa aatttcattc gagtgctcga
    38701 ttccaaaaac gactttctca cttttttact tacttttttc ttttaatact gtcagtcatt
    38761 atcatctatc cgcttttgat cacagcaagt actgccttca agtcaggaaa tattgcagct
    38821 tttagtttag atctttcaag tgactggact ttaggaaact ttactcggct attcagtgaa
    38881 acactttacg ggacttggta taaaaataca ttgatcattt ctgtcctaac aatgatcgta
    38941 caagtgactg tagtaacttt ggcgggctat acttatagtc gctatcgttt tatcggccgc
    39001 aaatcaagtt tgatgttctt tttagtgatt cagatggtac cgactatggc agcattgact
    39061 gccttttatg tcatggcttt cttattagaa gcgctcgata agtactggtt cttgacaatg
    39121 atctatatag gcggtggaat tccaatgaat acttggctga tgaaaggata ctttgacact
    39181 gtaccaatag atttagatga atctgctaaa cttgatgggg cagggcattt caggattttt
    39241 ttccagattg tattaccgct tgtcaaaccg atgctcgccg ttcaagcatt atgggctttt
    39301 atgggacctt ttggggattt catgttagct aagttcctgc ttcgaacacc tgaaaatcag
    39361 actgttgctg tcggcttaca aacatttatt tctgatgtga caaaccaaag agtctctctt
    39421 tttgctgctg gggcaatttt gattgcacta ccgatttgtc tattattctt ctttttacaa
    39481 aagaactttg ttaccggcct gacatctggc ggaacaaaag gataggagca gagtatcatg
    39541 caaacatttc cgttaaacta tttttccgca ttacgtacgc caactcaagt gtttgcggga
    39601 cgcaaactat tatcatggcc taaatttttc ttgatatttg tatttttagt ttccttgatg
    39661 gtcatgcctg tgactctttt ttatgccaat cagatccaag cgattccatt agaacaattt
    39721 ctatccgttc attcgctgat cgatgaacaa ggaacccaaa aattttctga acttgaatta
    39781 tctgaaactg ggttacaagc atcgcaacaa acgatagctg tgacccctga aattttggta
    39841 ggtgtgtctt tatctgagaa acaacaatcc gatcatggaa cttttattga ttttgagaag
    39901 gaacaatggg tgatccaaca aaaggataaa tctggtatac gccgctatac gatgaattat
    39961 agcccatctt tccaaccaga ttctgtacgt acgcccgaag atttccaacg atttttagaa
    40021 agagaatttt atgccagtaa tcgtccaaca atcattttat cttatagttt aagtcttggt
    40081 cttgtcctat ttgtcatgac gagtctcatt ttattcgggg cttccttttt cttgtggatg
    40141 acaagaaaaa gccaactttc atccattcat acattcaaag agagtgcaaa tttgatgctg
    40201 aatgttatgg gaataggttc catggcagca gcagttgtag ggtttatcca ctttgacttt
    40261 atcttgatgc taagtgtcca aacaacgata actgtcttgc tgcttttatg ggtttttgtc
    40321 aaaacaagat tcaaagataa gcgtgtcgaa tgacaagttt aagcgattta tgcgacaatg
    40381 aatgtgaaaa aaatgaaaaa taaagtagtt tttgaaaaat ttctgaaaaa attaaacttc
    40441 ttagttgatt cttttctttt ttagtattaa cgtaataggg tagttttttt aaggaggaaa
    40501 atcatgttag agaaaacttt atacaatcaa ttattttcac gttctttctc gctccctgtc
    40561 gaagtaaatt attgggacgg tacaacgaaa caatacggag atacggacac tcctacccaa
    40621 atcaaaatca atatccatga agaaatccca atgaaagaga ttacaagtaa cgcatctttg
    40681 gcactaggag aagcctatat ggatcatcga atcgagatcg agggaagtat ccaagcattg
    40741 atcaacgatg cttatcagca agcaaacagc tttttacgag gaaaagatta tctaaaatgg
    40801 ctgccaaaga aagaaaaaca tacgaaacaa aaaaacaaag aagatatcca tgcgcattat
    40861 gacttaggta acgactttta taaactttgg ctagatccaa ctttgactta ttcttgtgct
    40921 tatttcaata cacctgaaga tacgttggaa caagcacaaa tcaataaagt ccatcatatc
    40981 ttagacaaac tctttatcaa agaaggcgaa acgttgcttg atatcggttg tggctgggga
    41041 acattgatgt tcactgctac caaagagtat aatgtcaagg caactgggat cacactaagt
    41101 gaagaacaat acgatttgat taccaaaaga ataaaagaag aacatttaga agataaatgc
    41161 cgtgtattat taatggatta ccgcgaacta aaaggcgaaa cgtttgatca tatcacaagt
    41221 gtaggtatgt tcgaacatgt aggttctgaa aatcttgaag gttatttcaa agtggtaaaa
    41281 gatttattga aaccaaaagg aacagctttg atccatggaa tcagccgaca acaaggcggt
    41341 gcgacaaatg cttggatcaa taaatatatt ttccctggcg gttatgtccc tggtgttact
    41401 gaattagttg ataatattac aaaaaacgat ttgcaactga ttgatttgga aagcttacgt
    41461 cgtgactacc aattgacatt agaacattgg acaaagaatt ttcatcaagt ccaagatcaa
    41521 gtcacgaaag aaatgggtga ttccttctat cgaatgtggg atctatattt acaagcttgt
    41581 gcagcttcat ttgaatcaag taatatcgat gtgattcaat atttgcttgt tcaacctgga
    41641 aacaatgaca tcccaatgca tcgtgcataa ttgaggcaaa agtactgttt aaagactatt
    41701 taaaaagaag ctaagtttta tgatgctcca gaagtgaact tttttgtcta acttctagga
    41761 ttgtctctaa agcttagttt ctttttcttt atattttcat aataaaagtt tggttcttta
    41821 atttttaaac atgacagaaa attttttctt cgttatactt gtaatagaat actattttat
    41881 aacaaagggg atcgaatcaa tgaaaaaaac tgttatttat actagtttat ttgcagcttt
    41941 gtttttagct gcttgtcaat caaataccaa tcaaggcaca tctgggaata cgtcttccag
    42001 tagcgaacag cgctcttcac aggtatcgga ctcctcaaca aaagcaagca catcttcgtc
    42061 tagccagaca aaaagttcta gtgaagcaag tactcagtca gatatcaaag tgtctgcagc
    42121 tcgtgcgatc gaattgttcc aagataaata cccagatact gcaattacta gtttggaatt
    42181 agatagtgat tggggatctt acttctacaa gatagaaggt gtcgatgatc aaaaagaata
    42241 cgaagcaaga attgatgctc aaaatgaaaa aatggagaca gagaatccag aaactctaga
    42301 tcgagatgaa caaaatggtc aaaagaaaaa agaagatggt ctggatgttg ataaattgat
    42361 cacgattgag gaagcagcaa aaatcgccgt cgacaaagta ggaagcggta cggcaacaga
    42421 ttgggatttg gataaagatt taggtactac ttattgggaa gtcaaagtaa aagacggcaa
    42481 tcaaagtaca tcagtcaaga tcaatagcca aacaggcgaa gtactgtcaa ctgaacaaga
    42541 tgattaagaa tttcttcgga aaacaagagt tgatttttct tttagtcatg atacactaga
    42601 caaaataact tgtttgaagg agcgatttac ttgagtttag aaacgacctt tgccaatcat
    42661 atctttgcca atcctttgat gaatgcatct ggtgtccact gcatgactac ccaggaatta
    42721 gatgaactgg ctcattcaga agcaggagca tttatcacta agagctgtac gatcaatgaa
    42781 cgaaaaggaa atcccgaacc acgctatttt gatgttcctt tagggagtat caattctatg
    42841 ggacttccaa acttaggttt ttcttattat ctagagtatg ctcttgcgta tgaaaaagtg
    42901 caggaaaacc aaaatcaacc gttatttttt tccattgccg ggatgagtgt ccaggaaaac
    42961 ttggaaatgc ttgagaaaat agaaaaaagt gggtttaatg ggatcactga attaaattta
    43021 tcttgtccca atgtgcctgg aaaaccacaa ctagcttacg attttgaggc cacttatgaa
    43081 acactgaaag aagtattcag catcttttct aagccgctag gaatcaaatt gcctccttat
    43141 tttgattttg ctcattttga tcagatggct gatatcttga atcagtttcc attgacctat
    43201 gtcaatgcta tcaacagtgt gggcaatggt ttgtatatcg atacagaaca agaagcagta
    43261 gtcatcaaac caaaagaagg gttcggcggc attggcggag agtatatcaa gccgactgct
    43321 ttggcgaatg ttcgcgcttt ttatacacgt ttgaaaccag aaatccaaat cattggtaca
    43381 ggtgggattc ggacaggaca agatgcattt gaacatctgc tatgtggcgc aagcatgtta
    43441 cagattggta ccgagcttca taaagaaggg ccggaaattt tttctagaat cataaaagaa
    43501 ttaacgcaga ttatgtcaga aaaaggatat acgtcaattg acgaatttaa aggcaaatta
    43561 cgcaccattt catagcaaag agacgaaggt catgattgat cttcgtcttt ttttataaaa
    43621 aacactcgtc tgttcattcc tgtgcaagcg tactcattgt atgttagacg aacttgtggg
    43681 aaaataacag aggcaaaaag gaggataata tgaatgatgc aacagaatta gcaaaagagc
    43741 ttaaagaaaa agaatttagt tttcaagaac tagttacgga tatacagaaa aaaataaaga
    43801 agcaaaatca tgaattgaat gcgtttgtcg cgcttgaacc attcgagagt aattcccacg
    43861 agctattgat tccccagtca gaaaaagggc cattggctgg tatcccattt cctttaaaaa
    43921 tgctcggtca agaaaaaaag ggatggctag ctacttcagg atctcgttta tttgaaacac
    43981 accgagcttc tcatacatct aattatgtgc ggcaagcaga agcgataggt ctagttcctt
    44041 ttggacaaac caatgcgcca gagtttggtt tcaaaaatat tacagaccca gtcatctatg
    44101 gcccagcaag aaacccttgg aaccttgaat gtacaccggg cggatcaagt ggaggagctg
    44161 ctgctgcagt ttcatcagga atcgttcctt tagctggtgc tagtgatggc ggtggttcta
    44221 ttagaatacc cgcttcgttt tgcggattga ttgggctgaa accttcgcga gggacgatgc
    44281 cagttggtcc atatgcttgg agaggatggc aaggagctgc tattgatttc ggcttgacag
    44341 tttctatgag agacacggag gctttatttt atgggatgag aagtatccat tccggtgcgc
    44401 catatcaagt atcgccagtt gagtggcaaa cacatccaag aaaaaaacgg ttgaagatag
    44461 ctttttgtgt tgattcaccg atccactcac agattagtga agaggcagta cttgctgtaa
    44521 aaaaagctgt agatttcctt ggaaaagaag ggcatgattt gacagagatt ccttacccac
    44581 tagatggtcg taaactgatc acgtcttatt atcagatgaa tggcgcagaa acagcagcaa
    44641 tgatccgctc gattgaaaca ggcatccagc gagctgtcag aaaagaagaa cttgaaccca
    44701 tttcttggac tttgttccaa tatggtcaga agctctctgc cgctacttac atcgattctc
    44761 tgcatgtatg ggatgaagca gcaattgtca tggaaaacct acatcaagaa tacgatctat
    44821 tcctttcacc aacgactgca tttaccgccc ctaaaatcag cgaagatttg caaagtgatg
    44881 cgataagagc acagatggaa cacatcagtg attgttctga agtagaaggt ttagacgttg
    44941 tttcttccat gtttgaggag agcttgaaga tcacacctta tacacagttg gctaatctga
    45001 ctggacaacc agcaatcagc ttgcctactc atttgacaaa agaaaacatg cctctaggaa
    45061 tccaatttat ggctgccaga ggaagagaag atttgctatt tcaaatcggc tatctatttg
    45121 aagcaaatca tcagttccat ttaccaaatc catggaaata gcagaaacaa ggagaagaag
    45181 aatgaagaaa aaaagattgt ttgctctaat aactagtgcc ttttttattt taagcgcttg
    45241 cggtacagct gccgctaatc aggaagataa cagctggagt aatgtagaaa aaaagaaaga
    45301 attagtagta gcgacttctg gcacactgtt tcccgcatct tattataatg atcaaaatga
    45361 attagttggt tatgatgtag atttggcaaa agaggtggct aaacggttaa atgtcaaaat
    45421 ctcctttaaa gaatacaacg tggatggaca aatcacttct gtttcaaaaa gagaatcaga
    45481 ttttgctgcg aacgattttg gtatcacaaa agaaagagaa aaaaaattct ccctttcaga
    45541 gactttcaaa tattcatttg acagtatgat cgtacgaaaa aaagatgatt caggaatcca
    45601 ctcacttgaa gatcttaaag ggaaaaaagc agctggcgaa cccaatacta gctatatgcg
    45661 tttagcggaa aaacacggag ctaaattagt cacatatgac aatgccacga atgatcagta
    45721 tttaagcgat gtagcaaatg gtcgaacaga tgttattcta aatgattatt atctgcagaa
    45781 aatggccgta tctgctttac cagatatccc tgtaaaaata ctggagaacg tttatttcaa
    45841 tgaaaacgaa agtggttttt tattgaaaga aggaaatgat gctctgaaaa agaaaatcga
    45901 tggcgtatta aaagagatga aagaagatgg gacgacaaag aaaatatctg aaaaatattt
    45961 tcaaactgat gtttcagtca aaccgaaaga aacgatggct gagtcggtga gtgagtgatg
    46021 tactttcctc taattgattt ttccctcatg tggacttcac ttcctttcgt actgcaaggc
    46081 ttggtataca cattagggat tggcttcgtg tcgtttcttt taggaaatct ggtagggttg
    46141 cttttgacag ttttaggtct tttggattgg ctgcctctga atgtatttat tcgtttttat
    46201 ttatcgttct ttagaggcat tcccgcacta gtcctgctgt ttctgttata ttttgggtta
    46261 ccttatcaat taagtgcctt aacggcttca gtcatctgct ttacgattac cagcagtgca
    46321 tttattggtg aaatctatcg aggatcacta gcaggcgttt cttcaggtca gtgggacgct
    46381 ggtttagctt ctggtttttc tttctttggg gtattgaaat atatcgtttt gccgcaagct
    46441 tttcgaatct cagtaccggc actcagtaac gtagctatgg atctagtgaa aggtacttcg
    46501 ttagcagcaa caatcacagt gccagaaata tttcaaaaag caaagatcat aggcggtcgg
    46561 acattcgatt atttttctct ctatatttta gttgcactga tttattgggt catttgtatc
    46621 gggatcgaaa aagtgcaaca gtatttagaa aaacgttcta tgcatcagtc aatctgaaaa
    46681 atagaaatgg tgctagcgtg gctggcacca tttctatttt ttgtttcttg tatattctgc
    46741 ctagtatttt aaaaccattt catgtaaaat gtaaatcgaa aatatgttcg gaggtagaaa
    46801 acatgaagac cccgctaacc atcgaaatag aatcctcgtt ataccaatat tgcatcgagc
    46861 aaggcggctt ggttgtagaa gaagtcacga tgcctgatga ccaaggaatt gtagatacgc
    46921 ttagctgctt gacaaaattt gatggaagcc gagaatggcg atgctatgag ctgaaagtga
    46981 caaaagcaga ttttcgctct acagccaaac tgtcatttgt tggacattat aattattttg
    47041 ttttaccaag taaactatat gaagaagtaa aaactgaaat ccctacagga ataggtgttt
    47101 tagtgtatcg tccgtacacg gttgaagagg agatgcctgc ctctggtaca tttctgattg
    47161 cgaaaaaagc agcaaagaaa gagcttgctg taccggaaga agctttgaca aaccgcttca
    47221 tggcttcttt attcagggaa gtaagaaaag caaagcaaat ggagcgaggc gtaaaatatt
    47281 ttccaacaga tcaattgtac aaagaattga aaagacgttc agaaaatcaa gatccatttt
    47341 ctaacgaacg atattatgaa cggtttttag aggaaatagc tagtcaggca atcaatgagt
    47401 tgcaagaaga agttatcgcg cttcagcatg attatgaata tttgaggcaa caacagcaga
    47461 tccgtcggtt gcctacagag ccattggagt gaaaaatatg tactatcctt atttaagagg
    47521 aaaacaattt gacctattag cattaaaaga agcactgtcg cgaggattgc tctccaataa
    47581 aatccaacca gtgatcgaac cagtacgtga ttccgctacg cttaaaaatg tgatcgaatt
    47641 atttcaaaaa aagcaccatc gaatcgctgt gatccaaaat ccccaagtag gacaattcaa
    47701 attattcgat cagcatgtgc acagttggac agtcaaagaa aatagctctg tcgtccctgc
    47761 acagatctta acgccagaaa atctcagtga agtactggat tcacctccag cgtttcttgt
    47821 tttcgatgga cagcactacc caaaagatac agaggtgtgg aaacagttgg caggagttga
    47881 ttcgaaattt ttgataccag atactagtcg ctttcgcatc tggctgcccg aaaacaaaat
    47941 tgtgatccgt gactcttttc aaacgagaaa gcatgttgaa agttatgcag ataaaacaga
    48001 tgactttttt agtgatgact atcttttttt ccacgcagac ggatatattg gattttctga
    48061 tttcacgatc gaaggtagcc gttatttcga taaagggttc cctagccgag cgcttgctat
    48121 ccatctaacg tatattgatg cttacggcaa tatacgcgtg aaacattttg tctctgacag
    48181 taacgactct gctaaagacc aagcattgaa gtttcttgaa gcaggagata aaatgaaaaa
    48241 atggatcatg cgtcaccatc atcaactctt gattacttca ggaatgattg aattattggc
    48301 tctttaccag caacaaaaat tccctggact gggagtgttg aaaaagtggt cacttatgca
    48361 tcatttggaa cttattagtc acctgctaga tcatccaaaa gattggctta aatattattc
    48421 aaaagttcag gaactatatg aagtgatcca atgattagat aatgtaaatg aaaaaggaat
    48481 agaaaagagg aaactggatg tatattcggc aaacaaggaa agaagaagta gaagaagtgg
    48541 tagaaatcat taatgccgca gcggatttca ttgcttcgca aggctctcct caatggcaaa
    48601 acgggcagaa accaacaaaa gaaacgattt tgaacgatat tgaaagaaaa gaaagctatg
    48661 tattaatagt ggaagatcaa attgctggaa cagctgcatt agtctcaagc gtggatcccg
    48721 tttacacaaa aatagatggt agttggaaag aatcaactgg tccttattta tcgattcatc
    48781 gtgtcgcttt agcagaaagt actcgaggaa aaaaactagc aaaaccgttc ttagaacatt
    48841 tgttggcgat tgcagccagc aaaaatatcc aagatatccg tatcgatact tatccagcta
    48901 atgaaccgat ggaaaagacc atctattctg ctggattcac ttatcaaggg atgattgaat
    48961 ttccttttga acacggcgaa agaaaagcat atcaaataat catcaattga ctataaaatc
    49021 atctgagcaa agataaagac attaacacta ctgaaaaaaa caatcatatg atagccaaga
    49081 ggttgtaaca gaagtggcca gcttcaaaaa acaagaaaga atttccgaaa atgcttctca
    49141 aatttttgtg gaattttagc gtattttacg aaatagctgc tttctgaaac tcccttcatc
    49201 gagaggtcgt gacatagctt ttgtcacgac ctctcggctt atcctacgag tattattcga
    49261 tacatttata tcaataatcc tttttttatt ttatctgcag cttcttggaa cgctccatcg
    49321 tttgacacga ctaaaagtga ggggatagct ttcaaacgtt tcaactgtgc taaatcttct
    49381 acataaaccg ccagtctggc tgaaatctgt tcagatgaat ctccacgttt tttcaggcgc
    49441 gattgcaatg tttctttaga gatcgtaata aagataggac ggactaccgt cccgaaatac
    49501 tcatataaat gccaaaaacc atttgctgtc aaaacggcgt aacaatttcc tactgtcagt
    49561 ttctttttaa tttctgcttt actggaacca tatttatgtt gagcatattg gtcccactct
    49621 gcaaactcat ttcccttgat catctgattg aatgtattgt cagaaacaaa ataataatcc
    49681 tcttgatctt tttccccagg tcttggtgat cgattcgtgt aagtaatgat cttttgttcc
    49741 ggctgaaaaa tagcttctgc gagtgaagtt tttccagaac cagaaggacc gataaataca
    49801 tagcaataat tcattgtcat ttctcctttg atgattgaac aagtgaaaaa gctttggaaa
//
LOCUS       DACCDF010000013        48266 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCDF010000013 DACCDF010000000
VERSION     DACCDF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.013"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            125..1168
                     /locus_tag="IVK51_000710"
     CDS             125..1168
                     /locus_tag="IVK51_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP6430325.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1230..1694)
                     /locus_tag="IVK51_000711"
     CDS             complement(1230..1694)
                     /locus_tag="IVK51_000711"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP6430326.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            1940..2887
                     /locus_tag="IVK51_000712"
     CDS             1940..2887
                     /locus_tag="IVK51_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6430327.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(3098..5170)
                     /locus_tag="IVK51_000713"
     CDS             complement(3098..5170)
                     /locus_tag="IVK51_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6430328.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(5103..5480)
                     /locus_tag="IVK51_000714"
     CDS             complement(5103..5480)
                     /locus_tag="IVK51_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP6430329.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(5609..6169)
                     /locus_tag="IVK51_000715"
     CDS             complement(5609..6169)
                     /locus_tag="IVK51_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6430330.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            6319..7611
                     /locus_tag="IVK51_000716"
     CDS             6319..7611
                     /locus_tag="IVK51_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6430331.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(7671..8789)
                     /locus_tag="IVK51_000717"
     CDS             complement(7671..8789)
                     /locus_tag="IVK51_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP6430332.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IVK51_000718"
     CDS             complement(8803..10224)
                     /gene="arcD"
                     /locus_tag="IVK51_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP6430333.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IVK51_000719"
     CDS             complement(10470..11591)
                     /gene="mnmA"
                     /locus_tag="IVK51_000719"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP6430334.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNDKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            11995..12594
                     /locus_tag="IVK51_000720"
     CDS             11995..12594
                     /locus_tag="IVK51_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP6430335.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IVK51_000721"
     CDS             complement(12732..14018)
                     /gene="efmA"
                     /locus_tag="IVK51_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP6430336.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(14289..14633)
                     /locus_tag="IVK51_000722"
     CDS             complement(14289..14633)
                     /locus_tag="IVK51_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP6430337.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(14770..15906)
                     /locus_tag="IVK51_000723"
     CDS             complement(14770..15906)
                     /locus_tag="IVK51_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6430338.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(16095..17069)
                     /locus_tag="IVK51_000724"
     CDS             complement(16095..17069)
                     /locus_tag="IVK51_000724"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6430339.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(17149..18024)
                     /locus_tag="IVK51_000725"
     CDS             complement(17149..18024)
                     /locus_tag="IVK51_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP6430340.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSEKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            18154..20061
                     /locus_tag="IVK51_000726"
     CDS             18154..20061
                     /locus_tag="IVK51_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP6430341.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(20602..23880)
                     /locus_tag="IVK51_000727"
     CDS             complement(20602..23880)
                     /locus_tag="IVK51_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP6430342.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(24151..24696)
                     /locus_tag="IVK51_000728"
     CDS             complement(24151..24696)
                     /locus_tag="IVK51_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6430343.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(24708..25400)
                     /locus_tag="IVK51_000729"
     CDS             complement(24708..25400)
                     /locus_tag="IVK51_000729"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP6430344.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(25419..25703)
                     /locus_tag="IVK51_000730"
     CDS             complement(25419..25703)
                     /locus_tag="IVK51_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430345.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(25716..26279)
                     /locus_tag="IVK51_000731"
     CDS             complement(25716..26279)
                     /locus_tag="IVK51_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP6430346.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            26507..27169
                     /locus_tag="IVK51_000732"
     CDS             26507..27169
                     /locus_tag="IVK51_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP6430347.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            27293..28489
                     /locus_tag="IVK51_000733"
     CDS             27293..28489
                     /locus_tag="IVK51_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP6430348.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(28590..29966)
                     /locus_tag="IVK51_000734"
     CDS             complement(28590..29966)
                     /locus_tag="IVK51_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6430349.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(30003..30869)
                     /locus_tag="IVK51_000735"
     CDS             complement(30003..30869)
                     /locus_tag="IVK51_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP6430350.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(30958..31983)
                     /locus_tag="IVK51_000736"
     CDS             complement(30958..31983)
                     /locus_tag="IVK51_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6430351.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(32001..32675)
                     /locus_tag="IVK51_000737"
     CDS             complement(32001..32675)
                     /locus_tag="IVK51_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430352.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(32859..33752)
                     /locus_tag="IVK51_000738"
     CDS             complement(32859..33752)
                     /locus_tag="IVK51_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6430353.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(33826..35523)
                     /locus_tag="IVK51_000739"
     CDS             complement(33826..35523)
                     /locus_tag="IVK51_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6430354.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(35516..36829)
                     /locus_tag="IVK51_000740"
     CDS             complement(35516..36829)
                     /locus_tag="IVK51_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6430355.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(36829..40347)
                     /locus_tag="IVK51_000741"
     CDS             complement(36829..40347)
                     /locus_tag="IVK51_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6430356.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(40640..41098)
                     /locus_tag="IVK51_000742"
     CDS             complement(40640..41098)
                     /locus_tag="IVK51_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6430357.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(41396..41914)
                     /locus_tag="IVK51_000743"
     CDS             complement(41396..41914)
                     /locus_tag="IVK51_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6430358.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(41916..42533)
                     /locus_tag="IVK51_000744"
     CDS             complement(41916..42533)
                     /locus_tag="IVK51_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6430359.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(42530..43384)
                     /locus_tag="IVK51_000745"
     CDS             complement(42530..43384)
                     /locus_tag="IVK51_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6430360.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIILLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(43381..44292)
                     /locus_tag="IVK51_000746"
     CDS             complement(43381..44292)
                     /locus_tag="IVK51_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430361.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(44333..45667)
                     /locus_tag="IVK51_000747"
     CDS             complement(44333..45667)
                     /locus_tag="IVK51_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430362.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            45875..46705
                     /locus_tag="IVK51_000748"
     CDS             45875..46705
                     /locus_tag="IVK51_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP6430363.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(46949..47198)
                     /locus_tag="IVK51_000749"
                     /pseudo
     CDS             complement(46949..47198)
                     /locus_tag="IVK51_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(47389..47583)
                     /locus_tag="IVK51_000750"
     CDS             complement(47389..47583)
                     /locus_tag="IVK51_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430364.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
BASE COUNT        13936 a        10309 c         8574 g        15447 t
ORIGIN      
        1 tattcgaaac aagaggtcgt acaagacttt tgtcacgacc tcttgttctt ttgtcaaaat
       61 actggtttag tgctactatg ttcgagtatg cttttagtat ttattgacga aaggaaccga
      121 tcaaatgaaa tcattgtcac atccaataaa atctgtaaag gattcagcgg tccgcgaaat
      181 cttacccggt ttactgcttt cttttgccat cgctgccttc agcaggcttc ttgctattta
      241 tctgccacag ttaggtggag cgactattgc tattttattg ggtattttat taggaaatac
      301 tttttttaaa cagcctgttc tagcaatcgg cacaaaattt gctgaaagca aactattaga
      361 attctctgtt tttttactag gtgcaactgt cacagctcaa acgattgctc aaattggtgt
      421 aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact atcatcggtg cttacttgat
      481 tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg atggctggag gaaacgctgt
      541 ttgtggttct tctgctattg cttcaatcgc cccttccatc catgcgaaag aggaagaaaa
      601 aggacagatc atcacactag tcaatctact gggtactgtg atgatgttga ctctcccttt
      661 tcttggatta cagttgtttg gtggaaatct cattgaaaaa agtgcacttt tgggtggtac
      721 actccaatct gttggtcaag tagtcgctgg ggcaagctta cttgacaatt ctgttgtcga
      781 attcgccatg ctatttaaga ttctacgtat tatgatgctc gttgttgttg tctatctgtt
      841 tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa acgccaactg atacgcagaa
      901 aaaacgaaaa ttaccgcttc cttggtatgt attagggttt gtgatctttt gtttggtcaa
      961 cagcttgttt tctttacctg tagttattga ccaatcggca cattttatca gcagctggtt
     1021 tgagatcact gctttagcgg caattggtct gcgtttagac tttgcaaaat tcatgaaaga
     1081 aggtacacgc ttcttatctt atggattagc tgtcgggctt ctccaaacag tgatggcttt
     1141 actatttatt tggctgatca aagtttagaa tgaacatgaa aaaacagttg tgattctcgc
     1201 atggaacaat gtccgaatca caactgtttt taattttgca gatatccttc taacaacaac
     1261 aattggcgtt tgatttcaac cccatctatt gcgtttccca tatatcctgt cagttttcca
     1321 ttttgcccga tcactcgatg acaaggaata acgattggca atggattggt tcgatttgcc
     1381 tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa cggctacttc tttatacgtt
     1441 cggatttctc cataaggaat ggtcgacaaa gctttccaga cattttgctg aaaaggcgta
     1501 cctctcttta tagataaagg aacatcaaat tcttttcttt ttcccgcaaa atattcttca
     1561 aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg attcgctttc tgaaattggc
     1621 tgaaaagata cagcagtcaa tccggattca tctgcatcta accataaaaa gccgataggt
     1681 gtgtgtattt tcattttgtt tgctcctagt caatagactg gaacttcctc ctttttctct
     1741 atccaaaact acttgccaac taataaaaat tgttcttaaa agtttgataa accgaggaaa
     1801 catggtaaac taaatacgtt agtttacaaa aagaatacct gttcttttac gtaaaatcaa
     1861 attttaaagg tgttatttaa ggtttattaa attttgaatg taaaccatat ataaacatac
     1921 gattgaaagg atagaaacga tgaaagtatc aattgttgtc ccatgtttta atgaagaaaa
     1981 aactgttcct cttttttttg atgaggttga aaaattcaaa ggtgcccatg agttcgaata
     2041 tttgtttatt gacgacggat caaaagacgg tacgttagca gcaatcaaga agctagcctc
     2101 agcttatcct tatgtccgct ttgtctcatt ttccagaaat tttgggaaag aagcggctct
     2161 gtacgctgga ttgcaagctt ctacaggaga tttagtgacc gtgatggatg ttgatctgca
     2221 agatccgccc gaactgcttc ctgagatgat caaccggatc actacttctg atatcgattg
     2281 tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg atacgcagtt tctttgcgaa
     2341 aaaattctac cagttgatca ataaaatcag cgatacagaa atggtcgatg gcgctcgtga
     2401 tttccgcttg atgactcgtc aaatggtgga tgccgtccta gaattaacag aatacaaccg
     2461 attttcaaaa ggattattta gctgggtcgg atttaaaaca gaatatattt cttatgaaaa
     2521 ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg aaattattca actactcgat
     2581 tgatggtatc gtgaacttct cggatgcacc gttaacgatt gcttcattta tcggcgcttt
     2641 gtcttgtgtc ggttctggta ttgcaatttt gttcattatt ttgcgggcat tgttgtttgg
     2701 tgatcctaca tcaggatggc cttccatggt ctcgatcttc ctgttcattg gtggaatcca
     2761 attgttatgt cttggaatca tcgggaaata tattgggaaa atcttcttag aaacgaaaca
     2821 gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag ccaaatgaat tgaagcctca
     2881 ccaataatat acgtcaaatg aagtaaacat ttttttaaat gaaaaaggac tgtgacaaaa
     2941 gtcttgtcac agtccttttt tccgaataaa tggtgttcca gaagtagctt cttcggaaat
     3001 aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
     3061 agctgaccac ttctatcaca acctctttca tatcttatta cttcttcatt gctgctttta
     3121 gtgctgctgc taatgggctt tcttctggtt cttctgcttg tgcatatttt ttcagcaagc
     3181 gtcgttcttc atgtttggtc attttcttct tctgctcttt tttatcaagc aatttttctg
     3241 tcgttccatc gtatttacaa cggaaataag caccgttttt accttcgatg atttccattt
     3301 tccggtgaca ttgcgggcat cgatgattag agacttttgg atcttttctt ctgcggtagt
     3361 tgcaatctga attggtgcag acatagattt tgccgtcacg tgtgtttttc tcgcgcagat
     3421 tggccccaca ttctgggcat ttcttttggg taatcgagaa atcttgatat tcttgtttgc
     3481 ttgatttgat ctcattcacc aagcgttttg tttctttttc gattccattc aagaaggttc
     3541 ctgcttgttt ctttcctgca gcaatagctt caaggtcttt ttcccagctt tctgtcaaat
     3601 ctggtgttac taaagaggga tttaccaata ctaataattg cttaccttta ggagatactt
     3661 gcagtccttg aggggtacgt tccatcagtt cagatttgat tagtttctcg ataatctctg
     3721 cacgcgtagc tggggttccc agactgtatt tttccatttg accaagcaag cttgcttctg
     3781 acaatggttt aggcggtgca gatagttctt ttttgatggc gaaatcggga ttgattctca
     3841 ttcctttttc ccatttgact gtttccgtct cagtttcagt ttcgcctttc catcctgctt
     3901 ggacgacacg attttgccga aaaatgaatc gttctttacc aaattcaacc gtaacttttg
     3961 tttggctgac tttatggggt tgagcaaaca gtcctaaaaa acgagagaca atcatttgat
     4021 agatttttcg ctcttcatta cttaattttt cataacgtgc tctgttttca gttggcaaaa
     4081 gtgcatggtg atcggtcact ttactgtctt ggaatacttt tgtctgttgg acgatggctc
     4141 cgtttttcaa atactgcttg acctcaggag caaagtcgct gactgcttgc aatcgttctt
     4201 tcatcgtact tttcatgtca tttgtcagat atttgctgtc tgtacgcgga taagtaacga
     4261 tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt tttctttgca gaatagccat
     4321 agcgttgatt ggcttctctt tggatctctg ttaaatcata aggcaatggc gcagattccg
     4381 tttttaattt ttcttggact tctgtgacag ttcctttgct tgtactgatc aattgaacaa
     4441 gtttttgtgc ttcttcttgt gtttttaaag caaattgatt tttttgaagc attttggctt
     4501 gttcttttcc agcttgcaac gtgatcgtga aatacgtttg tggacggaac tgctcgattt
     4561 tcttttcttg gtctctcact aatgctagtg tcggtgtctg cacacggcct gctgataaat
     4621 tatccgcgta cttcacagtt aaggctctgg tcacattcaa tcctaccagc cagtccgctt
     4681 ttgcccgggc aagagcagat tcatacaatg catcatattc tttcgcaggt cttagttgct
     4741 tgaatccagt tttgattgct ttatctgttt gagaagagat ccataaccgc ttcactggtt
     4801 tattgaaatg cacatactct aagatccagc gagcaacaag ttccccttct cttccggcat
     4861 ctgtcgcaat gactgcttct gagacatctt ttcgctgagc cagctgcttg attgctttta
     4921 attgatgtcc tgttttagga agtggtttga ttcctagttt tttagggatc attggcaacg
     4981 tgtccatttg ccagctctgc cattccttat tcagatcttc cggcattttc aatcctaata
     5041 aatgaccaag ggcccaagta acgatcacct tgggtccttc ataataattt ttgtgctttt
     5101 gattagcacc aagcaccttg cttaggtctt tcgcaacgct tggtttttcc gctagaatta
     5161 gttgtttcat aaaattcctt tctgctttct actgtgatgg gctgatgatt tttcaacaac
     5221 gcaagtccct tgtattgctg taaatcatcg tcacactctt cacaccagcg ctcatctccg
     5281 tcattccaaa aggcgctgag aaagttactt tttgcattga tatattcata attttgatgg
     5341 atttcgttcc attgtttttc gtacgcttgt cgtgcttctt cgaaagactc gaatgtttct
     5401 tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc aaggctcatt gtccccgaac
     5461 atggtaatta cttgatacat actcgtgctc cttctttcag cttttctaga ctcatcttag
     5521 tcacttttca agaaagttgc aagaattttc tcttataaaa gggtcattta atgaacatct
     5581 taaaattgtt acaaatgaat ctgattgttt aaggcaaagt agcgataaag ttattgatca
     5641 tccaaaaata gctgtcttct ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac
     5701 gtaaggaaag ataaccgtgg atcatactac gaagttccct gcttcgatga actttttctt
     5761 ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat ctgattcgtt tcatggattc
     5821 catctaataa aacagcttca ttggtatgcg gtacactgat cagtaactca tacactgctt
     5881 gatttgaaaa agcaaaggat cgataagttt ctgcatagat ctgcagtgct tcctgaccac
     5941 tttttcccac aagagcccgt cgcagctggt cattaagttc ctgcagaagt ttcgctgtta
     6001 gcgctgtttt gacatcacga atatttttga aatggttata cagcgaggga gccttgatat
     6061 ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac atccatctct tctgctagtt
     6121 ctcgaaagca gtcgatgatt ttctcttgag acagatttct ttttttcatt tccatgtttc
     6181 ctctcataaa ataaatcctt cactctttca gtttaccacg aaaaagaaag agaaaaaaga
     6241 gactttcatt gataaactac atattgtagt ttataataaa gagctgtaga tgaaaatttt
     6301 cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa aattatttag gaaaagagaa
     6361 acttgggaac aaaacttgtt tgtcttatgg tttggaacat ttatggctgg tatcggattt
     6421 agtttagtca tgccttttat gtcactttac atagatacgt taggtaatta tagtacgtca
     6481 caattgaact tttggagcgg attgactttt tcatctactt tcttggtgac tacgcttatc
     6541 tctccttggt ggggaaaatt agccgaccaa aaaggaagaa aattgatgct gttgcgtgct
     6601 tcattaggaa tggcaatcgt catttctttg atgggttgcg tgactagtgt gtatcaatta
     6661 gtcgcattgc gtctgcttca agggattttt tctggttata tcagtaatgc cactgccctt
     6721 gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc ttggaacctt agcaactggt
     6781 tctgttacag gaacattact tggaccgctt cttggcggga tctctgcttc agcgtttggc
     6841 tatcgcccta cctttttcat tacagggatc atcttatttt tcgtcttctt gttgagtctg
     6901 tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag
     6961 attttcaaag acttgaaata tccacacgtc gtcatcggta tgttcgtcac aacgatgatc
     7021 atccaagcat ccaacaattc cattagtcca atcatcagtc tgtacattcg ccaattgctg
     7081 catggacatg ggaatgttac gttgatcagc ggaatcattg cttcgatacc tgggattgcc
     7141 actttgatag ccgctccaag atttggtcgc ttaggtgaca agattgggag tgaaagaata
     7201 cttgcgattg gtttgggttt tgcgattctg gtttatatcc caatggcatt cgtcacgaat
     7261 gtttggcaat tagctgcctt acgttttttg attggtatct ctgatgcttg tctgctccct
     7321 gctgtccaag cattgatcac aaaatattcg cctcatcaag ctgctggacg gatcttcagt
     7381 tataaccaat ctttccaagc aactggtaac gtagtagggc ctatgatcgg ctccagtgtt
     7441 tccagtgcat ttggttatcg tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac
     7501 ttcttgcttg tacgacacaa tacacaagag atccatcagc aagaaactgc agaagaaaaa
     7561 gcttcaggag tctccatagc ttcagggcac tctccttctc atagacaata agcgtaaatg
     7621 aaaaacactg cttgaacagt tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt
     7681 tttttcaaga actcagataa gaggaataat cccttttcca aggtttcttt tttacaacag
     7741 taacctagtc gggcatgctt cggtaaatca aaggcacttc ctggtactaa tagtactcct
     7801 gtttcttcta ataaattgat acaaaatgtc tggtcatctt cttcgatggc taatttaatg
     7861 aaagaagtag agacgtaatt gggagcgaca agttcaactt tcggttcatt ggctacccat
     7921 tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt ttcgcgccaa tattttttct
     7981 ttattcttca atgcatgtac agcaagatca tctgacagga ctccgccgca aatcatcgtg
     8041 tagtctcgat actttcgaaa aacttctgcc aactcaggtc cagtagctgt ccagccaata
     8101 cgaatacctg gaatggagta ggttttggat agggaattgg ttgctatacc tttttcatag
     8161 acatctacga tcgacaagaa ttcaccttta tccgttaaag gcgcatatac ttcatctatc
     8221 aatacatatg catctactgt acgagcaatt tctgcgattt tttctaaagt tttccgatct
     8281 agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg
     8341 actaaggctt ctagctgttc taaatcaaac tgccaatctt cttcttcctt caaatgaaca
     8401 aagtcaacag cggcacctaa agatttgggg atgtcatata attgctgata agtaggaagc
     8461 atcgaaacaa catggtctcc tgcttccact aacgcgtata gagctaaaag attagcacca
     8521 gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg tttgataaag agaagcgact
     8581 tcttgtttga aaacatctga tccttcgatc cagccatagt ccaatggttc gtttttgcgt
     8641 ttttcgaaaa atttacttac gctagtccca tctaagccaa cgagttcgtc taatgttaat
     8701 gcttcgatcg agctttgtgc aatatcatat accgctttct tttcccattt atttagccat
     8761 tcttctacac cgaaatttgc gattttcatg tttctttccc ccttatatct tgatccagcc
     8821 tacagcaagc aacccgattg aaagcagtgc ggccacacaa atcagcccaa tcattacttt
     8881 ttcttttccc gtaatttgat gtttggtttc acgacaagcg atccagtaga agcaaaaacc
     8941 aggaagatag gcaatactac ataaaaggac ataagaaaat cctgctaaaa tgatgcccat
     9001 caattcaaaa actacagcaa tcccaccgat cactacttgt tttttgattc cttcttttaa
     9061 agaaaactgg acttgataga tcccgacaag tagataacaa atcaaaattg ccgctgtaca
     9121 taaagaatag gcaaattgat aggcataatc agtaactaag aaagtcaata agaatagatt
     9181 agtcaagatt gcagtaacaa ctaacgaata agttggggca ttttttttat tcgtttttcc
     9241 ccaacctttc ggcaacagac cgtctcgagc catcaacatc gttgtttctg ctgggagcat
     9301 cgtccaagac aaccaagaac caatgatcga aataatcaag ccgatattga tcagcgctgc
     9361 tccccaattt cccacgattc cttttaaaat atttgccata gcaggttgtg aaatagttgc
     9421 taactcttct tgagataaga cgccgtatgg caagatcgaa gcaaggatat agataaacaa
     9481 taaacaaagc agaccaatga tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt
     9541 ggctaaaaca cttgcgcctt cgatccctac aaagacccac atcataacca tcaaacaacc
     9601 tttaatctgc aaccagatcg ttccttgctg ccctgttcca ttcactagat tatcagatac
     9661 gttaccccaa aaatcagcag tgaatatatt cactttaaat gcgataaata caaatattaa
     9721 aaataggaat aatggcacta atttacagat cgtcacgatt gtattgatga cgctcgctcc
     9781 ttcaactcca ttgtttacta ataaagtcag accccaaata aaaatacttg caaataaaat
     9841 agaaggaaca ttttgtccac ctttgaatat cgggaaaaat tctcccatgg cgctcatcag
     9901 catcgttgca aaagcaacat ttcccagcca cgcagacaac cagtaacccc aaccactgat
     9961 gaagccacct aatttaccaa atgaagcttc cgcgtaaccg aaaataccac catctagatc
    10021 tggacgcttt tctcctaaat gattcagcga taataccaaa gctaaaatac caatcccaac
    10081 gattatccat gaaaggatcg ctggccctgg agctgcagaa ctagccagat cacttgtgat
    10141 cccaaacaca ccgcttccga ttgacgaact gacaaccaga gcaaccagtc cccatttgct
    10201 aatacccgtc gatttctttt ccatttccat caccctcact tataaaattg aacaaatcac
    10261 ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt aacaaaaagc aactttattc
    10321 atattattgg atattatgaa ctttttacac aagtatttca agtataaaat gaataaaaat
    10381 taattttatt gcaaaaaaac agctatgcta tccaaaagaa aaggtgtttc ttttgttagc
    10441 catagctgtt ttgattcatt taatatcgct taaacgtatt gtaaaacttt tgtttcttga
    10501 tatgcgtggt cgatcaaacc accacccaga cattccattc catcataaaa tacaactgct
    10561 tgtccaggag tgattgcacg tacaggttcg tcgaatacga cttctgcacg attatcatcc
    10621 aataagcgaa ctgtcacagg aacgtcttgc tgacgataac ggaatttcgc tgtacacttg
    10681 aattcttttg gcattggttc attcgtcgta aagtggattt cacttgcatc caagcttgtg
    10741 gcatacaatg caggatgatg gaacccttgc cccacatata atgtgtttgt cgctagatct
    10801 tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc caccgatccc tagtccttga
    10861 cgttgaccaa tcgtataata catcaagcca gcatgttgtc ctttgacttc accatcaaga
    10921 gtgaccatat ttcctttttt tgctggcaga tagttgctca aaaattgttt gaagttcttt
    10981 tcaccaataa agcagattcc cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt
    11041 tcagcaattg cccgtacttc tgatttttcc atgccgccta gagggaacat cgtttttgaa
    11101 agctgttctt gtgataattg gctaaggaag taagtttgat ctttatcatt gtctactcca
    11161 cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt gcgcataatg tccagtagct
    11221 acataatcgg cgccaagatc cattgcataa tccaagaatg ctttgaattt gatttctttg
    11281 ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt attcggctag gaaatattca
    11341 aatacacgat cccaatattc tttttcaaaa ttcacagagt aataaggaat cccgatttga
    11401 gcagctactt tcgctacatc cttataatct tccgtggccg tgcatacacc gttttcgtcc
    11461 gtgtcatccc aatttttcat gaatattccg atcacgtcgt atccttgttc ctttaacaaa
    11521 agtgctgtta cagatgagtc gacaccgccg ctcatcccta cgacaacgcg tgttttgctg
    11581 ttgtctgtca tgatatcacc atcatttcaa ttagattctg aaacatctac gagttgaagg
    11641 tcgcaacttt tcagtatttt acattatacg tcatatttct gaaaatttca tcccttaatt
    11701 atcaagtcgt aaaaatactg ttctactgga cctattcagt acacaaacat gatagtcaac
    11761 caaaaaatca ctcatctttt gagcattcga tataaaaaat tggcatactc ataaaaaggg
    11821 ccagcctttc ggcaataagg aataatttta taaattgtga aaaactattt ttcaaaattg
    11881 cttcttatcg cttaaagctg cacgtctcat ttacaacctt ttctttttag atgttatgag
    11941 ttgctcagat aagtgatttt gtttactcta agaaaaaagg gaggcaaaag atacatgaat
    12001 tactttatca gtgatatgca ctttttccat gaacgcttat taggaaaaaa cgactttgct
    12061 ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt tgatcactag ctggaatgca
    12121 gtaataaaag aaaccgacca tgtttatcat ctaggcgacc ttgctatgca tccgcaatat
    12181 gaaaaagcaa atgcagaaat tttggaactg gtcactcaac tgcaaggaac aatccacttg
    12241 atcaaaggga accatgacag tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg
    12301 cttactgacg gaagtcccaa attttatttt tatgatgtag gtgccatcgt taaattcgac
    12361 catcaacaat attacttgac tcattatccg atgttgctcg ggcctaacga cactatccgc
    12421 aatcttcatg gacatatcca tcattacagc gtacctatcg agaacgatgt caatgtaggc
    12481 gtagacgcac cggaaagaga actcttaaag gaaaaactac catttggcac tccgttatct
    12541 gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt acgataggtc gtgaaaaaac
    12601 tgccctatat caagtattaa acaaataaag ataattactc atatttttca tttgagtagg
    12661 cttattgcca caggaacatt aggatcagaa atggagttgt aacaagactt ctgtcacaac
    12721 tccattccat actattcgtc tttttgctca tggtgttctt ttaccagttt tttctgtaat
    12781 tctttatcat aatttcgggc agctgacgtt aaaaataaaa cgattccgca taataaaatc
    12841 ccgattccgg aaaacaagaa cattttttct acccctaaac gatcagctaa tggtcctgca
    12901 aataataatc caattggacc tgccaggttc atcaaagagt tgaagttccc taagacacga
    12961 cctaaaacat tcgattcata actctgctgg atcattgcca tcagcagtgt attgaaatat
    13021 ggcgttgcac aacctgctcc tgcattcaag ataagaaagt acaagaaccc ttgactatcg
    13081 tttggaacca atccgcttgc accgatcgtc aggcccataa tgatgtaggc tataataata
    13141 ggtttcattc ggtctttcca tgttcctata aaactgatca gtgcaccgcc taagagcatt
    13201 ccgactgcgt agactacttc cactgctccc gcctgtccaa cggaaccacc aaagtaatct
    13261 agcgtcatca atggatacag actgattgct ggcatgaaca acagcatcac aaatgcacca
    13321 ttgatcgtta tgtaccataa ccctttatta tccatcaatt gctgcagccc aaattttgca
    13381 tctttaaaaa tagtaagcgg aacttccaat atttcaggag aaacttttgg gatcttcaca
    13441 aaaatcaata agccgacacc aaaaactgcc cccaatacat ctaataaaat aaggtaattg
    13501 acaggaacga ctgaaaacaa agccgcacct agagctggcg caatgatgaa attcgctgat
    13561 tggaccattc ctaactgtcc attcgtcctt gttatgtgag aagatggaac gattgtcggc
    13621 ataatcgact ggatcgttgg catttggaat gtttgtgcga ccgaacgcat aaacaaagac
    13681 acaaaaacta gccaaatcgg gaaggattct gaaatcgttc cgactactgc taaaataagc
    13741 gcgaaaatcg ctacaataat atccgtgaca atcaaaagag cttttttatc ccagcgatcc
    13801 actagcggtc ctacaaatgg gcttagtaaa accatgggaa tcattcctag taaagtagca
    13861 aaactcaaaa ttgtcgccga accggtttct cttgtcaaat accagatgat cgcatattgg
    13921 acgaccatac tagtgattcc tgacaaaaac tgtcccgata aaaagaacaa ataatttctc
    13981 ttccaattcg ttaaaacgac cgactgttca ttttccataa acatccctct tttctttttc
    14041 attttgtata gcgtttgcac aacaatcatt atacgggatt cttttttcaa aaaatacctt
    14101 tttccctaaa taacaatcgt ttcaatttaa atttctttac ttagaaacaa taaatcagca
    14161 aaatattaaa aataacaaca tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt
    14221 cgtccttaca aagttagtca tctaacaata ggccgtttca aaactagttg tctttttata
    14281 tccacagatc attcgacttt ttcgaataac ccacggctaa tcatcccatc catcagaaaa
    14341 taaaatttat cttggctcat tttctgtttt ggatctttga aaatattgac tgctttcaat
    14401 ccatttgctg tagtgatcga cattttcaat gtttgaatat cttttgatac agtgattttt
    14461 aatttgaatc cttctttgct ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg
    14521 cctccgtttg tttctgtaaa tccattatcg cgcaatgtat atctttttgc atcaggatgc
    14581 aaacgatagc taattggtgc gcccagtacc gttgctgttg tttcaaatgc catgattttc
    14641 acctcgataa aattagtttc tctttattat aaaagtttat cctgattttt actaggtttt
    14701 tcagttaagt tccctaaaaa atgaaggcgt tctctcagac atctgttcat ataagaatga
    14761 cttcactagt tattttttga cagcacgaac taattgatca aggaaatact ggatgtcgtc
    14821 ttcggtattt cccaaaccaa agctgatacg gatggattct tttagtatcg gtgtatcttc
    14881 tccatacatt gcttctaata cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc
    14941 agtagaaata gcgaccccat taagatcaag cttcatcagt agcaaatcat ttgcttcgcc
    15001 aattaagcga agattcaaca catgagggag tttattcgtt aaatcaccat ttacttgata
    15061 agggatttcg tttgcttcta atgtatcaag aatcaattgc tggaatcctt tatatttgtt
    15121 gttacgtgct tcacgttctt caggtgacag cagttcaact gcttttgcca taccccagat
    15181 ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa
    15241 tgcagggata tttactccgt ctcgtttata taagaaaccg acgccttttg gcccattgat
    15301 tttatgagca gagatactta ataaatcgat tcctaattct tctggatgga tcgtttggtt
    15361 tccgtacgct tgtaccgcat ctgtatggaa ataagctgga tgatccgcta aaagttcacc
    15421 gatttctttg attggcatca aatttccgat ttcattgttc ccgtacatca cagagaccaa
    15481 gatcgtatct tctcgtaacg cttttttgac ctcttcgatc gacagattcc ctttttcatc
    15541 tacaggcaga tatgtaacgt caaacccctt tgtctctaaa tatttcatgg tattaagaac
    15601 agcaggatgt tcgatagctg tagtgattag atgtctgcct tctttttgac gcgagaacgc
    15661 tgtttctaag atagctgtat tatccccttc tgttccccca ctgttaaaga tgatctcgtg
    15721 aggttttgct tgcagactgt ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc
    15781 gtggcgacca aatccatgta tgcttgaagg atttccaaac gtgtcttgca tgattgccgt
    15841 catcgcttca attacttgtg gatgcatggg ggtagttgcc gcatgatcca gataaatagt
    15901 tttcacttca ttcatccctt ccaaattatt gctaggcaca ttctagcata aaatagcgaa
    15961 caaaccaaag aagagctcat ttttgtctag aggacgtttc atgggcatag aaaaaaggag
    16021 ctgtgacaaa agtcttgtcg cagctccttg aagctgctca cttctgtcac aacttctttc
    16081 cctctttttt ttgtttattc gaattctttt tcttccagac ggaataacgg actcatcggt
    16141 gtattttcat ggatgcgttt gaccgcttca cccattaatt ctgaacatgt aacgattgtt
    16201 aagttttctg gatgacgatt ttcttctgtg aaaacagaat cagtgataca aatatctttg
    16261 attggtgcat tgtctaaaat acttttcgca tgatcggaca ataatccatg tgacgcacag
    16321 gcatatactt cttttgcgcc gttttctttt aatacatttg ctgctcgtgc aagcgttgct
    16381 cctgtgttca agatatcatc taccatgata catgtctttc cttgaacatc accgatcaca
    16441 taaccaccct cagttgaagc atcatctgca tgatctacga tggctaatgt gctgttcaaa
    16501 tattctgaaa gactacgtgc acgctgaaca ccgctgtttt ttggcgaaac aactacgatg
    16561 tcatctcccg tcagacctaa ttgacggtaa taatgagcaa acaacggcat cgtaaataaa
    16621 ttatctacag gaatatcaaa gaatccttgt acttgaaccg tgtgcagatc taaagtcagt
    16681 actcgagttg cccctgcttc ttcgatcaag ttagcgatca atttcgcagt gatcggttca
    16741 tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt aaggcaatac gacattgatc
    16801 gttttcgcac ttgctcgctt cattgcatcg atcatgatca gtagttccat atagtaatca
    16861 ttgacaggtg cattcgttga ttgaacgata tacacatgat cccctcgaat actttcttcg
    16921 atattgatgg aaatctcgcc atcactaaat tgcttcacga cgcttttacc taattctgtt
    16981 ccgaacactt tcgcgatttt ttcagctaat ggacggttgc cattcaaact aaaaatcttc
    17041 aacgtgtcat cctgatactt attactcatg tagtcctccc caaaacttct ctattcttcg
    17101 atagtttaac atatttttcc aagattttct gttaatttca gcaaattctt aaggaagtga
    17161 aactgttccg ttactccaac tggcaggaat gactttatga taatcgcccc agttgtcgtg
    17221 tactttgtaa taacgttctc ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg
    17281 attgccatat gtactgccaa gtactttcaa aataccaatc atcacaggtt tgccttcacg
    17341 tatacgtttt gtagctcgct gccatgaacc tagaagagtt gctcgtgcac gctcatgatt
    17401 gccattcttc tttaaaaaac tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag
    17461 tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt
    17521 tttcttgcga atctcatttg gaagcatcca ccccttacga tagtcttgat aataggcaag
    17581 gagaactgct gccgcgtaag taccacaaag atagcctgag ggggtagccc atttcttata
    17641 cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt gcgagttcct tttctgacct
    17701 tttttcttcg atcaaaacgg gaatatcttc ttttctgttg atccatctag ataactcgat
    17761 tggctcttca ttgatcagct tccatttttt acaagaaaga ccctcttgat aagtatattt
    17821 tgctggagaa tattctaccg gatgtcctga ttgatcgata atcacataac tttgatcttc
    17881 aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac tgcagctgct gagtgctggc
    17941 taatttttta atttttttat ctactttgaa atataaaggt ttatccacta cttgattcag
    18001 ttgggatcgc ctcctttttt tcattataag tgaaggattg aaaaaaacaa atcagtttga
    18061 ttgttcgaat tttcaatttt ttatgttcta tttgttaatt ccttcatttt gtaagataat
    18121 gggcacacga aacaaaaaag gagcgatcga tgaatgaaca aagaacgatt gaaacaagat
    18181 ttctatgaag cagtcaatga agaatggcaa aaaacagcaa aaatccctgc agataaacca
    18241 gctacaggcg gtttccaaga tttagttgac gggatcgacc aacttttgat gggagaaaca
    18301 gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca
    18361 tattatcaac tggcaaacga ttatgaccga agaaatgagt tgcatgctca accgcttcaa
    18421 cctataatcg aaaaagttga atcattgcag tctttcgacg aattgaataa gcaatttcca
    18481 gaatggctgt tggatacatt accattgcca tttaatctgg atgtagatgc agatatgaag
    18541 aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt tattccttcc agataagacg
    18601 tattatgcat cagaccatcc caacggtgca caactaatag gcgttttctt tgatatggct
    18661 gttcagttat tagagcttgc cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa
    18721 gcagtgcaat ttgataaatt gattgctcct catgtaaaaa gtgcagaaga aaacgcagat
    18781 tacagcaaaa tgtataatcc gcaatctttt gatacattta ttaaatacac agataaactg
    18841 gacttgaaaa aacttgtcac gggtttgatt gggacaactc cagataaagt gatcgtaact
    18901 gatccagtgt acttcgatca tttgaatgac atcctgacag aagctcattt tccgttattc
    18961 aaaagctgga tgatcattaa aacagtacgt tctcttagtg gttatctatc agaagaattc
    19021 cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag
    19081 aaaaaagcag cgtattacct tgcctctgga caattcgatc aagtagttgg cgattactac
    19141 ggaaaaaaat attttggcga agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg
    19201 atcgccgttt ataaaaaacg tctagaagaa aatgactggc ttggggatgc aacaaaagaa
    19261 aaagccgtca taaaattaaa taacttaggt atccaagtag gctaccccga taagatccca
    19321 gcattattcg accagttcaa aacagtacct gcagatcagg gtggtacttt gctttcaaat
    19381 actcttgttt tcagcaaact cacattaaaa gatcgtttca gcaaatggaa caaaccagtg
    19441 gatcgttcag aatgggaaat gagtgctgac acggtcaatg cttattatca tccatttcgg
    19501 aatgtgatcg tctttccggc agcgatcctt caagcaccat tttacagtct agaacaatca
    19561 agctctgcta attatggtgg aatcggagct gttatcgctc atgagatttc ccatgcattt
    19621 gataataacg gcgctttatt tgacgaatat ggcaacttga acaactggtg gacagaagaa
    19681 gatcttacac aatttcaaaa caaagcacaa gcaatgatcg aagaattcga tggtctgcct
    19741 tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg aaaatattgc tgatgcaggc
    19801 ggtctaagct gtgcattgga agcagcaaaa acggaatcag atctttctat cgaggaattc
    19861 tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa aagaatacca acaactttta
    19921 ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt
    19981 gacgaattct atgaaacatt tggtatcacg gaacaagacg ctatgtatct agcgccagaa
    20041 aaaagagtgc gaatctggta agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa
    20101 ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt cggcttattt ttcgaagaag
    20161 ttacttctgg aataccatgt attagaggtt gtgaaaaagc tgcttagttt caagtagtaa
    20221 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttgactta ctaccgagaa
    20281 actggctttt gaacaccgtt tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt
    20341 agagaaaaaa acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgagg
    20401 gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa aagctgtcct gcatcaagta
    20461 ataagaacag ccaatcaaaa gcagcaagga gaacctcatc tataaaaaga tagaaggaag
    20521 tgagacatct attggtctca tttccttcta tttttacttt cctcagtcaa ttgttttaca
    20581 tcataaaagg agaaattcca cttatttttt tcgctgtttc atgacataaa caactacacc
    20641 gattaacaaa attcctccag ctaatgctag caacgggtta ttaaattctc ccgtttttgg
    20701 gagtccttga gtgccatttt tttcactttc ttttgctgtc ctatccggat gagaaggttt
    20761 tccatttggt tttccatttg gtgattcatt tcctggattt tctctttctg gtttgtcatt
    20821 tcccgggggc acatcggcag tttctcgcac agtgatcgct ttttgctggg taacagattc
    20881 tgtatgagca gaccattttt ccccatcaaa tatttccgaa cgataagtga cttctagcgt
    20941 atattctcct tttgaaggtg ctgaaaaaat agcggtatga ttagaaaatt gagcacttga
    21001 ctgtgtttca acgatttgga tcttttctgg gaaataacgc atatcttcaa agacaggcgt
    21061 tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt
    21121 tccttctgca ttctccgcca caccatttgc ttcttgtata ataatcggat tattttgttc
    21181 atttggtttt gtctgatttg gttcttgtac gattgcatac tctggatatt ccgaagacgg
    21241 tgaggtttct ccgtcaaaaa tagcacctac agataatgga agaccatcca ctctttcctt
    21301 ttctgtttga cgattccccg gtttaatctg catcagtcca tgaacatgga tacttccatt
    21361 ttcatagcgc gtaacaggct gctgaccttc tttcgaacat gtatttagag actcgatcca
    21421 atcttttgat atggtatgtc caagagtatt ttctgattgt ttctctcgtt cattccaata
    21481 gtagttcaac gagtggtaga tttgatcttc gttttgattc aatgggcgga tctgctctct
    21541 catctcaaga ttctctgttc ggaaagataa gataccagaa gcatggtagt ctgtgtttcc
    21601 tgctgaagag gtataaagag ccacattata tgaaccgtta ttaaaacttg tactgctatt
    21661 gacgataata tctgttccgc tattggaatc gattcctttc gcaagatttt cgaaagacaa
    21721 actattgatc aattcatgag ggcccgttaa agaagaaccg cccattttga atccattacc
    21781 gttaccttct ccctcaattc ctggtaccca gccgtttcga taagcaactg agtttttgat
    21841 gattactttt ccgatcgaac ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc
    21901 attatgatac gcgatacaac cgtcaaatac atttccttct cctattgtca gtttggccgc
    21961 aaatccatcg gcatcttcaa atcctggatc ggcattttca aaagatgtac agttcttgac
    22021 taagttatgc gcgggccatg cttcgaaagg atcatttgag ctaccgctga tttggattcc
    22081 tgtatttcca ttccgataag catgaatctc ttcgatcaaa ttatgatttc cagaaatctg
    22141 taatcctttt tgcatagctg cactatttgt aatgtcaaac cctcgtaaac cccagtagtg
    22201 actgaacagt gtcatcccat ttccagttcc tttaaaatca agtacaggac gcttgttttc
    22261 tggatttttc tctgcaatca actgaatcgg gttttctctt gtcccgttaa cgccttttgg
    22321 aatcgtcaat cctctttcga aggaataagt tccaggcgcc atgacgattg tctgcccagc
    22381 atatgcgtat ctcaatgctt gtgtcagaga taatggattt tgtctgcttc cgtttcctgc
    22441 atctgaccct tctggtgtta cataaactgt cgaatattta ggtttacggt agtccactgt
    22501 atgaagaatc tcttttgttt cataactagt catctctacg tattcttctg gcggatagtt
    22561 tttatctggc gtaaaactga aagtaaattc gttcgttcct tccatcagtt ttgtctgata
    22621 atcaaattcc cgattcgcag tgatagccaa gttttttgcg ttcttaagca cctctcctgt
    22681 tgctttattt ttaattgtca ataatccgtc agcatttgct ctaaaggaca tagtatgatc
    22741 ttggtttcct gaatgattac tagaggtcac agtgtaaatc ggatcaatca agcgagtagg
    22801 tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa gcaacttcga ttccttctac
    22861 cttgatcgtc atattccttg ctgcagcaaa tcctgcataa ataaccgatt catctgaagc
    22921 gtataatttt tcccagtcat acatgatgtc agaagccatt tcttctcctg tttcaggatc
    22981 gtaataatat gcctctaaac cagtattcgt ttttttcatc tctacgtcca gtttagtaat
    23041 cgagttcgtg gccggaaaca gattcccatg accgtttccg aatatattat aagaacctgt
    23101 ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt gtttccactg agacactccc
    23161 aggagctggc gcagcttgcg gatcttcaga tgatattcct gtgattgccc gtgtccccaa
    23221 tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc acttcttgtc tatccgagtc
    23281 ccagacatat tcgatccgac tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc
    23341 aaacaaatga ttttttggta ctgcgtctct taccattaaa gcaaatcctt cttgggcatt
    23401 cgtataggtc caagattcta tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg
    23461 gattgcttga taataatagg tcattccatc tgatccgctt gactggaatt ttccgccgtt
    23521 attcgtggaa gagagccgca cgccgtcttc taatggttca atggcatacc tactagcact
    23581 tgtgctttct ccgaaaacaa cccctttcca ctcttcattt gcttcttcag attgcttttt
    23641 tgtcttttca ttttctgatt ctttcatcat aggatcattt ccttcttccg tatccatatc
    23701 ttcactagtt cttggtattt cctccattgt tgacatccct tcaatgttca cgtgtgcatt
    23761 ttcttcgttc acagactgtt gttcgttagc agcaacttca acgacagaaa ttaaaaccgg
    23821 ggtactgagc ataaggagat tcatcattag cgcactgtat tttatacgtt tttttaacaa
    23881 gtgtattcct cccgtaacaa attagaaagc gtttacttaa agtattacct aatttatttt
    23941 tactgtcaat aaaaaacaaa attataataa gtgtatttta taaaaagaaa aaatataact
    24001 taagttgttg ataataaaga gatttattaa cgtaaaaaga ataagttttt ttaaaataac
    24061 gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc tcttctttta ttattttgtg
    24121 ctttttattt tatcaaatca ctttgttttt ttataaaatt tctgcgccta gtgtggactc
    24181 aaaatgtctc aatgcccatt catgtccaac gggatcgaaa cttgccacgg catctttgaa
    24241 aatatggagt ttgtatccta gattatacgc atcgactgct gtatgcaaca cacagatatc
    24301 tgtacatacg ccggtcaaat aaatatctgt gatctggcgt tctcgcaaac ggatgtctag
    24361 atctgttcca ctaaaagcgg aataatgccg tttgtctatc caataaacat tttcctcttg
    24421 ctcatagtct tggtataaac tttttaaaga gccgtacaat tcccgtccac ccgttcctat
    24481 cacattatgc ggaggaaata agcggttctc cggatgaaag gaatcttttg gatcatgcgc
    24541 atcaatggca aaaacgacaa aatccttttg atcaataaac ttctttgtat gagcagtcag
    24601 ctttttttca attacttgtc ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa
    24661 atcatatgtg tagtcaatcg agatcaatgc gttcatctta gtcctcctta tacgagatgt
    24721 ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca
    24781 aagctttgtg tcgccgagtg atctgctgtg tcgctcattg cacgtacgat caaaaatggg
    24841 atattgaact gacgagctgt ttgtccaaca gcggctcctt ccatttcgca agctaatgct
    24901 tcagggaaat tcgttaagat ttctttgatt ttatctggcg agtcgacaaa tgtatcacct
    24961 gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca
    25021 ctgcgcaaat atgtgcttgc ttcataatat agaggcattc ctggtaattg tcctgggttg
    25081 tagccaaatc ctgttgcatc tgcatcaaaa taagctactt tgtctgatat aacgatgtca
    25141 cctacttgta agccttcacc gattccgcca gctgaacctg tattgatcac catgttcacg
    25201 ccgtattgtt ggatcaacag acttgtggta atagatgcta agacttttcc tataccggaa
    25261 cgtacgacga tcacttcgtg gttacctaat gaacctgaaa taaataaagc gcctgcgcgt
    25321 tcccatgata atggttcgct taggttctct ctcaaaattt ttacttcttc ttccattgcg
    25381 ccgataatac cgattttcat ctttttaatt ccttctttct atataaatgc gattgctaaa
    25441 aaaaccacac ataacattag gataacaata ataagagatt tcatcaaaaa agagttccat
    25501 ttggtcgtct tttcccgttc gctgatcctt gttttcgtga ttggtttatt ctttttgctt
    25561 tctttacgat aaaagctggc aatctttttt tcttcttgct gataagctgt ttctgctttt
    25621 cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc
    25681 gtaataagcg gtcctttggc cataagccga tccctctact ttcctttggt caatagttcc
    25741 cagtattgaa tcgcataaat cgtctttgca tcacaaatca actgatcttt catcgcttgt
    25801 tgcgcctctt caagagttag atgatacaac tccagtactt cgtcttcatc ttgtgctaaa
    25861 ggattctcga ccttctctac cccttgtgca tgataaatat gaaggacttc atttgcaaaa
    25921 cctggtgaaa gatacatcga agtaagatgg ctcagcgact tcgcacgata gccagtttct
    25981 tcttctaatt ctctagctgc tgtcatttcg ggattttgtc cttctcctgg atcgattttt
    26041 ccagctggaa tctccaaaat cactttttct aaaggttttc gaaattgttt gacaagaagc
    26101 agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc caggatgaaa taccagttct
    26161 ctcttcgaag ttcctccatc tggcaaacga acttcgtcta cagcaacatc aataattttt
    26221 ccgctataga tttctttgcg actgattgtt ttttcttcaa attgctcatt gttcagcata
    26281 tcttctcctg ctttctttgt aatcataact gctttttttt catttttcaa tttttctcaa
    26341 aaactcctta ttagtttacc atagataaac cttagatttt ctctaaatat agaaaatcct
    26401 tctttagtcc gatactcttt atagtagtcc gtatgctaaa ataagtatgt aatcgaataa
    26461 cgaaaagaat tcaatactca acgataaatg acatggaggg tttattatga taaaacgaaa
    26521 ttggccgtgg cttctagcat tggtcttgct atttctcttt gtccgaaaca atagtttttc
    26581 ttttgttcta ggacttttat tgataggtgt agttttagtt ttgtggggac tctttggttc
    26641 gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg actgacgaac ttgaaactca
    26701 ctatgaaaaa agcggtatga cagcgaatga aatttcattt tttcgacaaa caatgaatca
    26761 aacaaaaaat gagatcaatc aattgcaaat gaatatgcag cagacagcca aacttaaagc
    26821 aatcgatctc cgccatgatc cagtcaaagc agcgaaagct ttattcaaag aattggtgaa
    26881 agaaccaaac cgactgcatg aagcaagtca gtttctttat acgcatctgc cgaatctagt
    26941 tgatttgaca aataagtata atgagatcaa tgaccatgag atcaaaaaca aacagaccta
    27001 tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg tcagctttaa tcgcacaaga
    27061 ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac gtagagatat ctgttgcgaa
    27121 acagagctta aagcgtgata ataaatatga accagaacaa aaggattaac tcttttgttc
    27181 ataaaagaag actcggaaca ttcagatgat gtctgtctgt ttcgagtact ttttctaaag
    27241 tagagacatt catgtaagaa tcgagaacag attcaacaag gaggaatttt ttatggatca
    27301 taaaccgact gaaacaaaag atgttacacc agttaacgat acacttgaag atttattgaa
    27361 caacccgttt tcaactccaa cagatacgct gaccacttca caacaagcgg agattgatca
    27421 gctccaagag caacagacag ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga
    27481 acaagctaaa cagctagcag caaaaatcga tgctaatgat gcacagtctg tcatcagcta
    27541 cggatcggct gcccaagcaa aattaagtga attctcacaa tcgatgttga atcatgtgca
    27601 agcacaagat attggtcctg taggagattc cttaactgaa ctgatgtatc gtctccaaga
    27661 agccaatcct gacgaactgc gtgccggaga agggaacatc ttccagcgtg tcttcgggaa
    27721 ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa aagattggtg cacaaattga
    27781 caagatttca gtgaaattag ataaagaaaa agacggtctg ctaaaagata atctgatgtt
    27841 agaacagctt taccagaaaa acaaagacta ctttgatgca ctgaatattt atatcgctgc
    27901 tggagaattg aaaatggaag aacttcaaac aacgatcatt ccagaagcga tgaaaagagc
    27961 agaagaaact ggcgatcaga tggatgttca gattgccaat gattacacgc aattcctaga
    28021 tcgtctggat aaacgtacgc atgatcttcg cttagctaga cagatcacga tccagcaagc
    28081 accacaaatc cgtttgatcc aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc
    28141 tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg
    28201 tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa acaacaaacg acttattgaa
    28261 gaaaaattca gaaatgctga aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg
    28321 aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga
    28381 gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg
    28441 acacatggaa gaagatctga aacaaaaact gttggattta acccaataga tcacctttcg
    28501 cttgttacta aaacaaacgc ccaacaacag tttattacaa ctgttgatgg gcgtttttct
    28561 tatctttctt ctaacttctc cattggagtc tatttctcat tcatcttgct gtgtccatag
    28621 ccatagccaa aataaatgat cagtccaata accattacga taccgaacac gatccatgtc
    28681 acagtttgca gacgaagcat caatacaata caagtcaaga tggagacgat tggcaatagc
    28741 ggaacgaatg gcactttgaa ttctccagct tttggtttgc caaacatttt tctcaacttc
    28801 agtaagccta gactaacaag catcaaatag gcgagcgcca tgatattcgt tagttccgct
    28861 aatacattca atgggaaaaa tccggcaaag aatacagctg ccacaccagc ggcataagtc
    28921 gcttttttcg gtgtacgatg cttttgatcg atttctgtca taaacttagg aagtaagcca
    28981 tccttgctga cagcaaacag taaacgagca agtgaataca tcatcgagat cgttaccgtc
    29041 agtagcgtta aaattgcccc tacagcgatc accgcgccca ctgttccatg tcctacatac
    29101 ctcattgcaa aagccactgg gtcattgact cctagttcag tatacggaac gattcctgtc
    29161 aagatcagcg tcacgatgat gtaaaggaca gtagcaatca gtatcgaacc aatgattcct
    29221 cttggcacat ctttttgcgg gtttttgact tcctcggctg ccattgcaac ggcatcaaat
    29281 cccaagaaag caaaaaacac aagagctgca ccatctagca caccggaaaa accaaaaggc
    29341 atgaatggct gccagttatc aggtttaacg aaaaatattc ctacaatgat aaacaacgcg
    29401 atgataccaa atttgacata taccatggca ttattcaaac gtaatgcttt cttggcttca
    29461 agcgacacga tgtaagtgac gaatatcact acgagcaccg caatcaagtc aatgtatgta
    29521 ccattttccg gattgtaccc gccagttaat gcttgtggaa gttcggcccc taaacttttc
    29581 aaaaaacctt gcatatagcc agaccatccg gaagcaacag aggaaaccgc tagtaaaaat
    29641 tccccgatca acaaccaccc tgtcagccag gcagcaaatt caccaaatac aacatacaga
    29701 taggcatatg gcccaccaat cactggcact cgcgaagcaa attctgcgaa acttagtcca
    29761 gacaaaatga caacgattgc agctaagaca aatgaaagtg acagtgctgg accagcattt
    29821 tgattggccg caacccctgt caccacaaat attcccgtac cgatgatcgc accaattcca
    29881 agcatgatca aatcagacgt ttttagttct tttttcatct cactcgtatg attgagattg
    29941 atctctttct tacgaaacaa atccattgaa gttcctcccc attttaggtt ggaaaacagc
    30001 gattaaactg ttttcatata ccctaaaagg ataccaccta aaatgactaa aaggcaacca
    30061 acaataacat aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct
    30121 aaagtagaga taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat
    30181 ttgactgtta gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt
    30241 ttccacacaa actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc
    30301 attccgatac tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg
    30361 atggtatata caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa
    30421 tcaagcattt tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac
    30481 gcaccgctta caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc
    30541 cattcatgga agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac
    30601 aatggtagcc ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca
    30661 acactccaag ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga
    30721 ctaacaacga aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt
    30781 tgttgattgg catcgccacc aattttacca cttactaaac caatactacc ccatgccaac
    30841 atagggacta atgctattaa aatttccata accaatctcc ttttatttca catgataaga
    30901 aacttatcag cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta
    30961 acgtttttca ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc
    31021 ttcagttgat tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata
    31081 aagtcctaat cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt
    31141 atcaaagatc cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag
    31201 gctgactcct tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata
    31261 tttgatggca ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa
    31321 aacagtatga tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc
    31381 ctgactgcgt atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc
    31441 ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt
    31501 tgataattca ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg
    31561 aactagcaat ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata
    31621 atctttttgg tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat
    31681 cagctgattg atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata
    31741 attttgcaaa ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct
    31801 caagtaacca cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc
    31861 aattgtgctc aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc
    31921 aataaaaaac agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt
    31981 catcttaacc gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata
    32041 tatccttgga tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat
    32101 gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt
    32161 aagatctttc cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa
    32221 tcaatgatct ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg
    32281 tggctcatca tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg
    32341 atttttgcaa tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg
    32401 ttcatagcca tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact
    32461 ttcgatactt cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata
    32521 tctagtaata caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg
    32581 gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg
    32641 atctcttcgt cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt
    32701 gctactacaa tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt
    32761 ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc
    32821 agcttaaatt tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt
    32881 tgtttttgtc ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat
    32941 atacctagaa ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt
    33001 gaagagactt cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc
    33061 acggtgtaag gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat
    33121 gtatttgttt ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga
    33181 ccatacactt caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt
    33241 tttttctcag caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt
    33301 ggaaaagagg tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca
    33361 tcaaataaag gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt
    33421 tcgtaatatt cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg
    33481 acttgtccca ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc
    33541 actgctaatt gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc
    33601 tcgatccttc tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa
    33661 actgcagctc cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg
    33721 gtttgaggtc tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact
    33781 gcagcaacct ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt
    33841 cattttagat ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa
    33901 gaatgccaag agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac
    33961 tctttctggc cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata
    34021 agagatgata aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc
    34081 catggtctct ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt
    34141 tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc
    34201 tgattcataa acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc
    34261 ttcattccac aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt
    34321 ttcaaacttt cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc
    34381 aaattcatca tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc
    34441 tagttctgtg gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc
    34501 taatctacct aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac
    34561 aaacccgttg cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc
    34621 ggctcgcact tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat
    34681 tttgtaattt ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat
    34741 aggtgctgtg atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt
    34801 ttcttgttct ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt
    34861 catctgttta tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta
    34921 tgtgtctttc catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg
    34981 catcataaca accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa
    35041 gaaaagatag actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa
    35101 taccgcttct ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa
    35161 aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc
    35221 gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac
    35281 gtcaaacaaa tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa
    35341 tccgctattc ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt
    35401 cacttcactt ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat
    35461 ccctattccc gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt
    35521 catttttttc tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc
    35581 ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg
    35641 attgacctgg attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg
    35701 tctcgttata aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt
    35761 ctaattcagc catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca
    35821 cccaaaattc agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt
    35881 ccacaccttt taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg
    35941 taacaaggcc ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat
    36001 tgtctgctgg tttgatccat tgatttttca agtcattagc aggagccata tctaaataat
    36061 atttcccccc ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt
    36121 tttcagttgt tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga
    36181 catttttagg atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa
    36241 ttggcaaaat agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt
    36301 ctggatcaat cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag
    36361 gtgctttgat acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta
    36421 ccgaagtccc tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt
    36481 tcgtttcagc gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt
    36541 catgataata atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct
    36601 gtgaaatgat atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat
    36661 ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt
    36721 ccacgacatc ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa
    36781 gcggtagtaa aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc
    36841 ctccttttta gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa
    36901 tgcagcaagc aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc
    36961 aggatcgttc ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc
    37021 taactcttgc tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac
    37081 ttttagtgta cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc
    37141 tcgataacta atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt
    37201 cacttccttg atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc
    37261 gtttggatct ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt
    37321 ctcaattgaa aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag
    37381 ttccaaatcg attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat
    37441 ttcatagcct tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa
    37501 cttaccttct tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg
    37561 tgcgacagat tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt
    37621 taaatcgaca tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa
    37681 acgtttcgat tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga
    37741 agaatccccc actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc
    37801 taattttcta actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca
    37861 taaacgatcc tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg
    37921 cttgagttgg atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc
    37981 atcaggttct ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt
    38041 ggtagtttct tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac
    38101 tttctcacca tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct
    38161 ccctttctct tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat
    38221 ttccagtccg ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt
    38281 actgccttca attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc
    38341 cagtttgaca tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga
    38401 aagcttgtga tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac
    38461 tctcgaacca gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt
    38521 ggctgcatat tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg
    38581 tccagcgatc tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat
    38641 cggcatcttc gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg
    38701 ttcggcttcc ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg
    38761 ctcgccattt tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat
    38821 ggaagaagat gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt
    38881 ctgaatatag gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag
    38941 ttttacttgg tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt
    39001 ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc
    39061 ttgtccatga cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc
    39121 tggatctaga tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt
    39181 aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat
    39241 atcctccaag gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac
    39301 gccatcttct tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg
    39361 ttgtctcccg ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt
    39421 ctcttgatac ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt
    39481 ttcttgcgaa gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg
    39541 agtcgcttta ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc
    39601 ttctggacgg ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt
    39661 attcccagaa aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc
    39721 ttccatcaca gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat
    39781 cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt
    39841 tctattttct ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt
    39901 aagcggagtt cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg
    39961 attgatatca tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc
    40021 tgtactatta tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag
    40081 cagatcttta ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt
    40141 ggtcccagcg gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat
    40201 tggactgatt tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt
    40261 tgcatcttca ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt
    40321 tttgatgtac gccccgttat aatccaagta actgttttca aaattccgtt catttcccca
    40381 aagctgaacg gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt
    40441 agtagcatag tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat
    40501 caagttctct ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt
    40561 ccaatttcct aacacttttg tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa
    40621 ccaccacttg aagaggtagt tacccttcgt tcacatcggg gatcttttct gtttcaaaaa
    40681 tcaatgtgcc ggtttctgct tcttttgaat aaacttttcc gacataccat tcttgaccat
    40741 cgatcgtctc gacttttagg tcttctccat cttcatcata ggaggataga cctgttccac
    40801 gaacgttttt tacggtacca atcccggcgg ctgccgcatc gattgctaaa cgtattctac
    40861 cttcattggc actttccttt ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt
    40921 caatatactt cccttcaatt tttgcatagt cctcatctaa gaatacctca tcctcctttg
    40981 tttctgttga ctcagcagct acatccaggg tcgtcatatg atagactgac tggaaatatg
    41041 gtccaaatag caaactgaca gttactaact gcaaaagctt caattttaac cctttcacat
    41101 ccttatactc ctttcaaaaa tatttgtctt ctttcacaac aatcaacaaa acaatgccca
    41161 tctaattgta atttaattta attttaattt taaatcaaat gatatccttt ttagttaaaa
    41221 aacaccaatt atacaacatt tattattaaa attaaattat aaaattttat aacgttctca
    41281 tttaacaaaa cttaagttat taagacaaat gaataaacaa ctacgctttc ctcgaaacaa
    41341 aaaaataaag cacaaatgcg catttacgca ctcatgcttt attcgtacaa ttaattcaga
    41401 taacggcagc gcataggatg ccaataacac ttcctaaaat agctcctcct aaaatgtcag
    41461 taggataatg taaaagtaaa aaaatcctcg aataagcgat caaacaagca aataaaaagg
    41521 ctaatattcc tgcaactgga cttaattcga acaacgcagc tactgataaa aaggctagcg
    41581 ctgtatgtcc tgaaggaaaa gaagaagtct tcggtttact tgtgtaaagg acaagccgac
    41641 gatttttttc ataaggtctt ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa
    41701 aaaaatagat caaactgccg ataagaatgg ctagaccata ttctctagta tcaggtatca
    41761 agactaaaac agcagcaagg agcaaccaaa taaaaccacc attagagata ttactgataa
    41821 atttcaaaaa attgattttt cgactatccg ttttccgttg cttggcgaac ttgttgataa
    41881 tactatcatc gattttagca atcaaacgca acatgttatt cctccttttt aagaaccttt
    41941 tcttgcgtac gatggataaa aagcaggact tgaaaataaa acaagaaact gaaacctaat
    42001 aaagcaaaat agattggtcc tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc
    42061 aaatagaaaa gcgcgctttt gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa
    42121 gcttcttttt gttccagttg aagcaagatt ttgtaactaa agagaagcgc gcttaataga
    42181 agcaaaaaca acgtagtata catcaatgta tagccaattc ccccacccca atagcggaaa
    42241 atctgatagt cgatcaatag aagaagaaaa taaacacttg ctaaactatc ttgctttcca
    42301 taaagagaga agttctcacg aaactttttc cagaatagtg aaccgagctt tttctcttca
    42361 ttttttttgt aatgataaat catatacaga actgttcgac ttgcagttcc taatgtgaaa
    42421 acaggcaaga cgcacaggaa ccatagtagc tgtaaatgga taagacgtgg aatccattca
    42481 atgatttcta agaaataatc attggctttt ttaccaatcg atcgattcat catccttttg
    42541 aacctcctgt caaatccatc ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa
    42601 tcgtcgggat caagacaagt gttgctcctg ccatcagata attccattgt gtcgttgctt
    42661 ggttctgaaa aacttgcaag ccaatctgta acgtgtattt ttcttgatct tggatatata
    42721 acagaggacc taagaaatca ttccaagctg cgttgaagga attgatcgca atggtaatca
    42781 aggcaggttt cgtcaatgga agcatcaaat gactccaaat atacaaatga ttcgctccgt
    42841 cgatttcagc agcttcgatc aattcattat tgatcgataa gtaaaattga cgcatcagaa
    42901 atatattgaa cgcattgccg aagaacgatg gaacgatcag gggcaaataa gtacctaccc
    42961 agccgatctt ggagaataat acatattgag ggatcatcgt tacaaacccc gggatcatca
    43021 tcgtagaaag aaccaaagca aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc
    43081 cataagcaat aaaagaattt gaaaggacat taccgatcac tactagcaca gtgatgaata
    43141 atgtatttct tgcatagcgc caaaaaggaa aggcggcaat cgtatccaga tagttttccc
    43201 atacgatttt ttctgggaaa aaggtcggag gataacgaac gatttcttcc attggtttca
    43261 atgaagtaaa caccatccaa agcaatagaa tgatcaaaat gattcctatt actgtcatga
    43321 ggacataact tagtattttt tttgtttgcc gattcacact ggtctttttg aaataatcca
    43381 tcatttgccg tcacctccag aataatagac ccacttaggt gcaagcttca aattgacaaa
    43441 cgtcagaacc aacacgatca gaaacaggat cattgacata gccatcgcat aacccatatc
    43501 aaaagctacg aacgccttgt tccacatatg cagattataa aaaagcaaag aatttgcagg
    43561 tccgcctgtt ccatttttcg tcatgacata agcttcttga aaaatttgga aagaaccgat
    43621 cgtagaggtg atcacatcgt aaaaaataat aggagtgatt aatggcaaag taatactttt
    43681 gaactttccc caaacgcctg ctccatcgat ttctgctgat tcatataaag atctaggaac
    43741 attttgtaat gtagctaagt aaagtagcat tgctcctcca gaactccata ttttcatcaa
    43801 aatcaacgca ggtttcgtcc attgtggatc aaacagccaa gctggtccag ggatatggaa
    43861 ccagcccaga aatgtgttga tcaatcctgc agatggagaa agcagctgca tccataagat
    43921 gtagactgct actcctgata agactgccgg taaatagaaa gctgtacgga atacactgat
    43981 tcctttgatt ttttgattga gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg
    44041 tacattgaag atgacgaaat aaacggtgtt gtataacgat ttccaaaata aatcatcgtg
    44101 aacgaacatt cttttgaaat ttgctagacc aatgaaattc atcttggatg tcaaattata
    44161 atcggtgaaa ctaccaataa ctgaaaaaag cataggccct agcataaata ccaagaaccc
    44221 gataataaaa ggcgagataa ataaataggc ccatcttgct tcttttttct ttcggataga
    44281 taaacgcttc aaattttctt gcctcctctg tacctagaca gtcaaaaatg gattattgat
    44341 tttgcttgac taagttttct actgcttttt gtgcatctgc caatccttct tttggtgatt
    44401 ttgttcctaa aagaatctgg tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat
    44461 ctaaaggaac tggtgtgatc accgtatttt taaagttttc gtccgccatt tgatagatca
    44521 tttttccttt atccgttagg ttgtcattat tcactaaatt ctcattggct gtcttgctcg
    44581 ccatgatatc aaaacttttt tcgccgaatt tttcttgtac ttctggagaa gaaagatatt
    44641 tgatgaattc ataagaggcc tcaggatctt ttgctccgta tggtacttcc aagacgaatc
    44701 cgccacccca cgaccaatgg cctgaacctt cttctttttc tgggagctgc atcacgccaa
    44761 aatcaaaatc atccggtgca ttttccatca aactagagta ataattgata ttttgggcac
    44821 gcatggctac taatccggaa ataaatggat cagatacacc agacccaaat tcggcttcta
    44881 atttattgat cgtatttttc ccataataat cttgccagtc ttggatccac tccaaggctt
    44941 ccactttatt tttagtatca atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt
    45001 tatctgcgtt tagcgcccat acatcagccc ctaaattcca taatggataa aaaccgatgc
    45061 gtgtaaattc ttttccttct tttttatcta gtttacgtgc tgtttcttct aattcatccc
    45121 aagttgtcgg tagatcttgt tcactgattc cagcttcttt aaataacttt ttattataaa
    45181 aaatgacttg tgtatctgta ttgaatggga tcgcataagg atcaccttca tattctactg
    45241 tatcccaaag ctgcggataa aaatccttag aagtgtcttt ttctacatat ttggagagat
    45301 tagttgcttg ctgtgcttct gctctttggg caacggaatt tatgtcctgt acaataacat
    45361 cgggtgcatt tccagctgtt attgcagata aagatttagt ccagatgtca ccccatggct
    45421 gatacttata atttacatgg atctcgtttt gtgattgatt gaaatcctca atgatttctt
    45481 cgatagcttc ttttcgtgca ccggatcccc aaaaggacca gaaatcaata ttttttcttc
    45541 catcacttgt tttttcagaa gaatttgctg cacttgaact ggcatcgctt gcattacttg
    45601 atccgcatcc tcctaataaa aacaatgctg aacttgccag caaccctgta aacaataatt
    45661 ttttcatatt ttacctccaa attttaatgt aaccgatgcc ggttttaata taacgatatc
    45721 attttgttct gaaaataaca atttataagg tatgattcta aaaaaattat taatgatacc
    45781 aatactttat ataattcgcg agcgctttct ttgaattttt taatctttcg tagcagaatg
    45841 agaatgtatt aaaaatagat taggaggaat tttgatgttt agacaccaga aagaattgca
    45901 gtttgaagca aaaccagatc gtccgaatcc attaatcgcg aaatatctgc aggagctgat
    45961 cggcggccaa tatggcgaga tgagtgtagc tatgcagtac ctgttccaag gctggagctg
    46021 ccgaggagaa gaaaaatata aagatatgct gatggacatt gcaacagaag aaatcggcca
    46081 cgtggaaatg ttggcgacaa tggttgctcg tttattagag ggtgcgcctg ttgaagatca
    46141 agaagcagcg atgcaaaaag atccaacaat cggtgccatt atctctggaa tgaatccaca
    46201 gcacgcgatc gtagctggct taggacctcg tcctgctgac agcgtgggca atccatggag
    46261 cggtgcctat atcattgcga gtggcaactt gttagcagac tttagagcta atttaaatgc
    46321 cgaatcacaa ggaagacttc aagtagcacg tatttacgaa atgatcgatg atcgtggcgt
    46381 aaaagatatg ctgtctgttc tccttgcaag agatagctat catcaaaatc tatgggaatg
    46441 tgcagtgaaa gaattagaag aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg
    46501 tgacaaagaa cgaaaagaca ttgcttatga cttctataac ttctcagagg gagaccaaag
    46561 tagtgaaggt tcttgggcta gtggcaaagc actggatggc gaaggcgaat atcaatggtt
    46621 gaaagaacct aagatcgaag gcgctgcacc aaaattagaa ccagtaacac caaaaatgta
    46681 tgggacacct ccattcaaga aataaatatc tacccgtaga aacacccgtt ttttgttcca
    46741 cacgctttgt gagcaaaact gactaaagtc attaatcata accacccttc aaaagggtgg
    46801 ttttctctag agctataagc tcaaatcacc ggccagcgcc taaagacgct ggctttcata
    46861 ttttatgttc aagccatatt gcctttgcca gttttgccca cctcttaaaa ggtttatgta
    46921 ctactcctta gcccttaaaa ggatcgtctt actctcgtac actcagctta tctaacgcac
    46981 tatcatgttt ttcttgttct tgaatgtact ttttgatggt cgcttcattt tgtcctactt
    47041 ttgtcacata gtatccttcc acccaaaagt gtcgataccc aaatttatat tttagatttg
    47101 cacaatctct tgatgatctc ctgaatattg gctcgatatt gattataaat tatttttctt
    47161 ctatactttg gtgtgaatac aatgtggtat ttacacatct actttgtacg ggctaaacta
    47221 tttcccttta ttgctccacc cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc
    47281 tcaattaggt ctaaagacaa taaatctaaa aacccgctgc ttcactgaca gaaacaacgg
    47341 gttttcagaa aaaaatgaaa tgaaaaagat ctcttacggc aatctatttt atttttttga
    47401 ttttttataa atggaaaatg cttcaagaag tccaagaaat aaagccgtat aaaaacaaga
    47461 acctatgaaa tctttattta ttagatattc aacagaaatc ccgataacac tagccaacaa
    47521 tatatttatc agaagtgtaa aataaggatg ctttttatag aatagaacta gtgttgtttt
    47581 catagactct cctttcttca tatttccaat taatcacaaa ttcacggcat tactagaaaa
    47641 attaaagggg cgcagaagtg gaaaaatact cttcgacttt ccctacaaac caggccatct
    47701 tgtgtttaat aactatacca aatctaaggt acctctaaat ctaaattcca tttgtggcgg
    47761 tgacctccaa tttttaactc ttctatctcc catccagtat acataacctt gatacttcga
    47821 ccacttatat atcgaacaca caaataccta tccattgcag gagtactacc taacttagtt
    47881 gctacttgca tctaaataac agctgaaaag ataggactta ctcttagtaa agtatctgaa
    47941 ctattaattt ttaaactatc atttttatcc tcaactacat gcacatctgt tgcaacttca
    48001 ctttctgtct catccagata aatgattaag gaatcgggaa ttgcaatatc ttcagctgac
    48061 gctggaatag ttgagctaaa ttagtcatca tatctttatt ataaaataac caacagcaaa
    48121 aaatacaatg aaaaacaaac ttggaactaa tgtatcatta ataaatgttg aaaaattcag
    48181 caactcatta ctgtatgatt ttagcagccc aaccaacata taccctataa aagctaataa
    48241 gatggttagg aatcctttag aatttt
//
LOCUS       DACCDF010000014        44687 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCDF010000014 DACCDF010000000
VERSION     DACCDF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.014"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(290..1087)
                     /locus_tag="IVK51_000751"
     CDS             complement(290..1087)
                     /locus_tag="IVK51_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP6430365.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1084..2268)
                     /locus_tag="IVK51_000752"
     CDS             complement(1084..2268)
                     /locus_tag="IVK51_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6430366.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2293..3540)
                     /locus_tag="IVK51_000753"
     CDS             complement(2293..3540)
                     /locus_tag="IVK51_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP6430367.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3755..5500
                     /gene="dnaX"
                     /locus_tag="IVK51_000754"
     CDS             3755..5500
                     /gene="dnaX"
                     /locus_tag="IVK51_000754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP6430368.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5520..5834
                     /locus_tag="IVK51_000755"
     CDS             5520..5834
                     /locus_tag="IVK51_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP6430369.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5859..6455
                     /gene="recR"
                     /locus_tag="IVK51_000756"
     CDS             5859..6455
                     /gene="recR"
                     /locus_tag="IVK51_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP6430370.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6519..7163
                     /locus_tag="IVK51_000757"
     CDS             6519..7163
                     /locus_tag="IVK51_000757"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP6430371.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7178..7507
                     /locus_tag="IVK51_000758"
     CDS             7178..7507
                     /locus_tag="IVK51_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430372.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7521..8459
                     /gene="holB"
                     /locus_tag="IVK51_000759"
     CDS             7521..8459
                     /gene="holB"
                     /locus_tag="IVK51_000759"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP6430373.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8495..9319
                     /locus_tag="IVK51_000760"
     CDS             8495..9319
                     /locus_tag="IVK51_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP6430374.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9312..9659
                     /locus_tag="IVK51_000761"
     CDS             9312..9659
                     /locus_tag="IVK51_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP6430375.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9728..10600
                     /gene="rsmI"
                     /locus_tag="IVK51_000762"
     CDS             9728..10600
                     /gene="rsmI"
                     /locus_tag="IVK51_000762"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP6430376.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10709..11830
                     /gene="dinB"
                     /locus_tag="IVK51_000763"
     CDS             10709..11830
                     /gene="dinB"
                     /locus_tag="IVK51_000763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP6430377.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVK51_000764"
     CDS             complement(11884..12486)
                     /gene="nrdG"
                     /locus_tag="IVK51_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP6430378.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVK51_000765"
     CDS             complement(12801..14990)
                     /gene="nrdD"
                     /locus_tag="IVK51_000765"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP6430379.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15316..16083
                     /locus_tag="IVK51_000766"
     CDS             15316..16083
                     /locus_tag="IVK51_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430380.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16073..18115
                     /locus_tag="IVK51_000767"
     CDS             16073..18115
                     /locus_tag="IVK51_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430381.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18396..18545
                     /locus_tag="IVK51_000768"
     CDS             18396..18545
                     /locus_tag="IVK51_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP6430382.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18556..20070
                     /gene="dltA"
                     /locus_tag="IVK51_000769"
     CDS             18556..20070
                     /gene="dltA"
                     /locus_tag="IVK51_000769"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP6430383.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            20067..21272
                     /gene="dltB"
                     /locus_tag="IVK51_000770"
     CDS             20067..21272
                     /gene="dltB"
                     /locus_tag="IVK51_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP6430384.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21317..21550
                     /gene="dltC"
                     /locus_tag="IVK51_000771"
     CDS             21317..21550
                     /gene="dltC"
                     /locus_tag="IVK51_000771"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP6430385.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21553..22821
                     /gene="dltD"
                     /locus_tag="IVK51_000772"
     CDS             21553..22821
                     /gene="dltD"
                     /locus_tag="IVK51_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP6430386.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22962..24044
                     /locus_tag="IVK51_000773"
     CDS             22962..24044
                     /locus_tag="IVK51_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP6430387.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            24044..24847
                     /locus_tag="IVK51_000774"
     CDS             24044..24847
                     /locus_tag="IVK51_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6430388.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24917..25657
                     /locus_tag="IVK51_000775"
     CDS             24917..25657
                     /locus_tag="IVK51_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6430389.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25668..26672
                     /locus_tag="IVK51_000776"
     CDS             25668..26672
                     /locus_tag="IVK51_000776"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP6430390.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26717..28561)
                     /locus_tag="IVK51_000777"
     CDS             complement(26717..28561)
                     /locus_tag="IVK51_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430391.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28773..29291
                     /locus_tag="IVK51_000778"
     CDS             28773..29291
                     /locus_tag="IVK51_000778"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6430392.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29419..30102
                     /locus_tag="IVK51_000779"
     CDS             29419..30102
                     /locus_tag="IVK51_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP6430393.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30411..30484
                     /locus_tag="IVK51_000780"
     tRNA            30411..30484
                     /locus_tag="IVK51_000780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30445..30447,aa:Arg,seq:tct)
     gene            30643..31002
                     /locus_tag="IVK51_000781"
     CDS             30643..31002
                     /locus_tag="IVK51_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP6430394.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31125..32756
                     /locus_tag="IVK51_000782"
     CDS             31125..32756
                     /locus_tag="IVK51_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP6430395.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32818..33459)
                     /locus_tag="IVK51_000783"
     CDS             complement(32818..33459)
                     /locus_tag="IVK51_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP6430396.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33741..34049
                     /gene="rplU"
                     /locus_tag="IVK51_000784"
     CDS             33741..34049
                     /gene="rplU"
                     /locus_tag="IVK51_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP6430397.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34076..34420
                     /locus_tag="IVK51_000785"
     CDS             34076..34420
                     /locus_tag="IVK51_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP6430398.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34433..34726
                     /gene="rpmA"
                     /locus_tag="IVK51_000786"
     CDS             34433..34726
                     /gene="rpmA"
                     /locus_tag="IVK51_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP6430399.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35133..37967
                     /locus_tag="IVK51_000787"
     CDS             35133..37967
                     /locus_tag="IVK51_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6430400.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38158..38649
                     /locus_tag="IVK51_000788"
     CDS             38158..38649
                     /locus_tag="IVK51_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP6430401.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38752..39741
                     /gene="manX"
                     /locus_tag="IVK51_000789"
     CDS             38752..39741
                     /gene="manX"
                     /locus_tag="IVK51_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP6430402.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39774..40577
                     /locus_tag="IVK51_000790"
     CDS             39774..40577
                     /locus_tag="IVK51_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP6430403.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40598..41512
                     /locus_tag="IVK51_000791"
     CDS             40598..41512
                     /locus_tag="IVK51_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP6430404.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41595..41969
                     /locus_tag="IVK51_000792"
     CDS             41595..41969
                     /locus_tag="IVK51_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP6430405.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42108..42353
                     /locus_tag="IVK51_000793"
     CDS             42108..42353
                     /locus_tag="IVK51_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6430406.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42525..43589
                     /locus_tag="IVK51_000794"
     CDS             42525..43589
                     /locus_tag="IVK51_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6430407.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43835..44260
                     /locus_tag="IVK51_000795"
     CDS             43835..44260
                     /locus_tag="IVK51_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6430408.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44253..>44687
                     /gene="nusB"
                     /locus_tag="IVK51_000796"
     CDS             44253..>44687
                     /gene="nusB"
                     /locus_tag="IVK51_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP6430409.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKEL"
BASE COUNT        14194 a         7700 c         9380 g        13413 t
ORIGIN      
        1 ctctgagtca caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac
       61 ttgtgaccga ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc
      121 tgcatcaagt aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg
      181 acttatcacc gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga
      241 cacgattttt gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc
      301 tgccgccacc gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc
      361 ttccaggagg tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat
      421 cggttttttc agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg
      481 atttcaattg ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca
      541 aaacaacagc aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag
      601 tttcttcgtc tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt
      661 tagacaggta agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat
      721 catctaagat cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg
      781 aaatgtagat ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc
      841 cgactgcgtc tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag
      901 tcgttacgat caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca
      961 aagattggat ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg
     1021 catgagtagc caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaattttt
     1081 tcatcatatc aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag
     1141 agcggtcagt cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg
     1201 tccattcatt gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag
     1261 ccataccggc agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac
     1321 accggtcaat gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt
     1381 taattcagat tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg
     1441 gaacccggct aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac
     1501 gccttcgacc atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa
     1561 agaaagcttg cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc
     1621 tatccgccat atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc
     1681 tgtagcccaa taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa
     1741 agctttggga acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc
     1801 tttctcgaca gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga
     1861 tagtacgacg ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac
     1921 gatctctgca ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga
     1981 ttcgtccgct gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt
     2041 catatcggca agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc
     2101 tttgctttct tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc
     2161 ttcttctgta tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa
     2221 agtcaacgga ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc
     2281 tttctaaata aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa
     2341 tttagggatg ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag
     2401 cttaggctga acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga
     2461 attttctgtc ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg
     2521 attttgtttt tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag
     2581 ataagaacca tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc
     2641 ttcatttttt ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg
     2701 tacgctagga tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc
     2761 atcggtgtaa gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg
     2821 ccaagaatca tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac
     2881 ataagaaatc cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag
     2941 gaattcagct aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg
     3001 gattgcgttt gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc
     3061 ccgtaagaat aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc
     3121 gaatttgatt ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt
     3181 gacgccgcca aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt
     3241 gaacatggaa ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc
     3301 gatgattttt ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt
     3361 tccgtctgtt aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg
     3421 gactttgaca ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc
     3481 tagccattga tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat
     3541 aaaaaattcc tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat
     3601 aggaagatgt gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt
     3661 taaatacctg aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa
     3721 gagatagtga attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt
     3781 ctggcgttcg cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa
     3841 aaatgctatt gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac
     3901 cggaaagacg agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga
     3961 cggtgagcca tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga
     4021 tgtgatcgaa atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga
     4081 tcgggcaaat tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca
     4141 tatgctttca acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag
     4201 tgtcatattc atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg
     4261 gactcagcga tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt
     4321 tatcttgaaa gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc
     4381 tgcagaaggc gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga
     4441 aggaacaatt actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat
     4501 ggacagttac ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa
     4561 ccaaattctc gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg
     4621 ccgtgacttg ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat
     4681 cagaagtaca tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat
     4741 aaagattttg agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt
     4801 ggaagtcgct acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt
     4861 agcatttgca gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa
     4921 cgaaatcaag gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca
     4981 gcaaacaaat cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa
     5041 agttctgcgg gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt
     5101 gctgatgagt ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc
     5161 tggacctaca ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa
     5221 tgacgaagaa ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc
     5281 gcctgatcct gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac
     5341 gcaggcacaa gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat
     5401 cgagttgctt cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga
     5461 actgtttggc tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta
     5521 tgatgcgtgg aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag
     5581 aaatggcaga agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg
     5641 aattggtcaa agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag
     5701 aagtcgttga tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg
     5761 atgcgatcgc acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt
     5821 tgccaggatt ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg
     5881 cgaaactgat cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac
     5941 tcgcctttta tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt
     6001 taagtgtaaa acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc
     6061 cctgcgaaat atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc
     6121 cgaaagatgt aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc
     6181 atggtgtgtt atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt
     6241 tgcagcggct gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag
     6301 agggagaagc aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga
     6361 ctcgtctagc acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt
     6421 tattgaaagc tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga
     6481 tacaatataa aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa
     6541 tcgaggggcc tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga
     6601 aagaagtagc aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg
     6661 aagagatccg agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag
     6721 cactgcttta tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg
     6781 caaaagggaa aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg
     6841 caggccgacg catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta
     6901 ctacgccgga ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca
     6961 agaaaaacag acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc
     7021 gtgtacgtca tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg
     7081 atgcaagaaa aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt
     7141 atccgcaatt ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat
     7201 ccaagacaaa gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac
     7261 aaaactatca tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat
     7321 tgaagatgac cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa
     7381 gcaatttgta tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc
     7441 agtagaagta gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca
     7501 attttaggag gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg
     7561 tgtatcagca gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg
     7621 aaggggagaa aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct
     7681 gtacccaaat gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa
     7741 agaaagaaca tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc
     7801 agatcagacg tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt
     7861 ttttgatcaa agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct
     7921 tggaagagcc ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc
     7981 tccctactat tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac
     8041 tgatccatcg attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct
     8101 taacaaacag tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta
     8161 aggacttgat ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct
     8221 atgtccaaaa aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat
     8281 ctgtcttgat gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg
     8341 gcaaagtgaa acagataaac cagataattg aacggatatt gatcgcagag cagaaactac
     8401 gcagcaatgt cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc
     8461 ttgccgcaaa ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata
     8521 tagagatgca ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga
     8581 caaggtatta gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa
     8641 agagattgat cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca
     8701 agctgaatta gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc
     8761 aaaagaaaaa gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt
     8821 tgatcgaaat aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt
     8881 ggtaaaagat ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg
     8941 cgatgaagca aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc
     9001 ttttttaggc gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt
     9061 gaatccaaca aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag
     9121 cgaagaatac gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc
     9181 tgatggtaaa gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct
     9241 gactggacgg gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt
     9301 aggtgagatg aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt
     9361 tgagaaatac attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa
     9421 aaaatacaac tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc
     9481 aagttgcaca agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc
     9541 ttgaaaaaat ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg
     9601 acaatgacga gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac
     9661 caataacaag aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt
     9721 gtgacagatg tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct
     9781 tgtaccgacg ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca
     9841 agaatccgat cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt
     9901 tgaaatccat acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca
     9961 attgattgct ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc
    10021 ttccatcagc gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt
    10081 gatctctatc ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc
    10141 tcaaccattt ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt
    10201 gaaaaagcta aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac
    10261 tgctacattg acgaatttct tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga
    10321 attgacaaag gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat
    10381 cgaagaacat ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat
    10441 gccagtgacg gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat
    10501 cgcttcaggc gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa
    10561 aaaacaagaa gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata
    10621 atgaaagact aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat
    10681 gttcttgtat aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga
    10741 tacatcaaga aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg
    10801 agatcatcct gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg
    10861 tgggcgtgga gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc
    10921 aatgagtgca caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca
    10981 tcataaatac agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat
    11041 tgtggaacca gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg
    11101 caagtcagcc atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct
    11161 aacatgttct gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca
    11221 aaaacctaga ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt
    11281 gccaattgaa aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg
    11341 gatttataca ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg
    11401 aaaaatgggc tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt
    11461 tacaagagag cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga
    11521 agaagcagtg ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag
    11581 agtccaaaaa catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt
    11641 gaccaaaaga gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc
    11701 aagccttata tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac
    11761 tttgacgaat ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca
    11821 agaaatataa aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg
    11881 tttttatatc aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt
    11941 ttcccaaatc acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt
    12001 gctacttccg cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac
    12061 aaggatatcg atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc
    12121 aaaggtatag cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg
    12181 tcgtacgact tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa
    12241 tccttgcacg taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa
    12301 tggtttgcca tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa
    12361 gcagccacta acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt
    12421 gtagtcagca atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt
    12481 tctcataaaa aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct
    12541 actcgaccac ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct
    12601 tattttgata gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac
    12661 ttgtcatagc ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag
    12721 cccaactaaa cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc
    12781 aggacagctt tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg
    12841 cggccatgta ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact
    12901 acatcacatg tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc
    12961 ggtttgaaat caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca
    13021 agatacccga ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt
    13081 tggagcatag ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc
    13141 ttctcaaatt ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg
    13201 tttgtataat attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa
    13261 cggcagaaac ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca
    13321 tattcatccc cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa
    13381 gcttttgctt ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac
    13441 aatccaatgt agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa
    13501 tcggtacgtg aaagacgttt cccgaaagca ccatacatat ataaaattgg cgcatttgct
    13561 ggaatggctt ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag
    13621 cggtcagaca gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt
    13681 ggcaaattca atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt
    13741 tcgtctttcc acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt
    13801 aattcaacga ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc
    13861 aatgctagtt gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc
    13921 aaagtgaaga tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt
    13981 cgaatctgta aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat
    14041 cctaatgaag tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc
    14101 aaactttgca tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg
    14161 cgttctggcg aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct
    14221 gcaaaaggag caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac
    14281 gctacattgg caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat
    14341 tcgacttcgg cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag
    14401 cagcaattcg tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct
    14461 ttttgatgcg catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg
    14521 atcccggctg tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg
    14581 ttcacgacag tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct
    14641 ttactgcgag caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat
    14701 tcgttttttt ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt
    14761 tcagcaaacc ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca
    14821 taagtaagtg gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg
    14881 atcttttgtt catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat
    14941 gtctctggct gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac
    15001 tcctgctaaa ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc
    15061 ggctatatat agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata
    15121 ttttattgtg aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat
    15181 tttgctgaat aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa
    15241 cataagagca aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa
    15301 agaggaggag aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc
    15361 agcaaacttt ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag
    15421 tttgttggga ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc
    15481 acgatcaatt tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg
    15541 aaaaacagtg agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc
    15601 aatttgatcg ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg
    15661 atgactaaag aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa
    15721 gaactgctca aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct
    15781 gctagagcac ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg
    15841 gactcgaagt cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa
    15901 gcaacgatac tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta
    15961 tttattaaag atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc
    16021 tttgagaaag taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta
    16081 taaactagcc gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag
    16141 tttgacttta tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt
    16201 agtcagaaga gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat
    16261 cattactgtt gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag
    16321 gcaaaaggaa ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat
    16381 atatgtgtta gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg
    16441 tattattttt tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac
    16501 tagtccattt tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat
    16561 ccttttagca gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag
    16621 ttttggagaa aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt
    16681 actcgcagtc atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag
    16741 ggaaatcact tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc
    16801 ttttttaatt tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg
    16861 gttatttttg atcatattga gtaaatggaa gatttattac tatggtatca attttttgat
    16921 aatgggtaac acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt
    16981 cattctcgga atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc
    17041 ctaccaggag aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa
    17101 aatcaagccg attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta
    17161 taaagtcatt gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa
    17221 tctgatcacg gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat
    17281 ttctttgaaa tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa
    17341 tatttcatta tatggatcta gtattggact gccagataat caagaattga agattcaaca
    17401 aatgcttcca agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt
    17461 agatgatacc gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt
    17521 atcaggtgga aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg
    17581 gctcaatgct gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag
    17641 cacaaagaaa cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg
    17701 tttgaatttc tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa
    17761 tatttttgtc acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac
    17821 agtccggcaa ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct
    17881 ggggattcca caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt
    17941 tccacccatg atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta
    18001 tataaaaact gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta
    18061 tcttttgctt tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac
    18121 ggaataagac aagataataa atagaactaa attttgaata ccatttattt ggaataagag
    18181 gttgtgacac gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa
    18241 attataaaga aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac
    18301 tgttagtaaa aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt
    18361 acactttgtg tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa
    18421 aattgaatta ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt
    18481 tcctgattta tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat
    18541 tttaggagga agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata
    18601 aatgaacctg atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa
    18661 caagcatcag acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt
    18721 gtcgtttttg gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct
    18781 ggccatgcct atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt
    18841 cgggtggcta aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg
    18901 gttcctgtca taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat
    18961 cccagaaata gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact
    19021 ggagaaccaa aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg
    19081 atggaagact ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt
    19141 gatttatcgg tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta
    19201 aataaagcag ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg
    19261 aatgtgtggg tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat
    19321 ggagaaaatg tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa
    19381 actgcagaaa gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca
    19441 acagaagcaa ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat
    19501 gaccgactac cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt
    19561 aaacctgttt caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa
    19621 ggttacctga acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca
    19681 agttaccata caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga
    19741 cgtatggatc agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat
    19801 tatctgctcc aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca
    19861 aaagtacagc agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt
    19921 cagttgacta aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct
    19981 cagaagttcc attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa
    20041 aaattaacag ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc
    20101 cgttttattt ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat
    20161 gggaaaaaag attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct
    20221 ttggaggtaa ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga
    20281 tacttgtatg gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg
    20341 ccgccgttat tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac
    20401 atggaaaagc atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac
    20461 aaatgatcat ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc
    20521 agtttctgct gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg
    20581 aaaaagatca gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg
    20641 tccaaaatat ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag
    20701 tactgcttcc tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag
    20761 taggttatat gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat
    20821 ttgcagtcgg tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt
    20881 ttttatcatg gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt
    20941 tccgtgatta tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa
    21001 gcaagatcgt aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc
    21061 atggattgac gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga
    21121 cagatgcatg gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta
    21181 cgcatggctt agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt
    21241 caggattttt agataaactt ttttttagct aacgaatagt gacatattaa tttatataac
    21301 aaaggagaaa aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac
    21361 agatgaagta agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt
    21421 agcatctgtt caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc
    21481 tgaattcgag cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt
    21541 gaagggttga gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt
    21601 ggcttactgt ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg
    21661 attagacgtg cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat
    21721 tatgcattat ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc
    21781 agtccatttc atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg
    21841 ctgggagcac ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat
    21901 gctatgaaaa acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt
    21961 gtaaaaactg attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt
    22021 tcaatgaaga aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca
    22081 aaagtaaaag agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta
    22141 cctttacctg aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat
    22201 gaagtattca gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa
    22261 cgattaccaa aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa
    22321 cgagaaacga cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga
    22381 gcccacgagc cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa
    22441 ttttctgatt ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc
    22501 atcatcccac cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta
    22561 caaggatttg ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct
    22621 gattttgtca atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg
    22681 aaaggctggt tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact
    22741 gctagtaagt atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca
    22801 gataaactac aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt
    22861 ttgaatattc tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa
    22921 tggtataatc acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac
    22981 agctacttat tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag
    23041 aagtattcag agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca
    23101 gtgtgattgc caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg
    23161 acgaagttgg ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc
    23221 ttggaggttg gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag
    23281 ggaaaatcgt ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa
    23341 gaaataaacc ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag
    23401 aagccaaaga gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac
    23461 gaatgaatgg cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg
    23521 tttctctgcg agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag
    23581 gaagcgatgt gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa
    23641 atcccgatat tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac
    23701 caaaagaagc agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta
    23761 tgatcccgca taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc
    23821 cattccaata tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa
    23881 gagatggtgt tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat
    23941 cagtgattca tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac
    24001 ggcttgatag tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc
    24061 tatttctgaa tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac
    24121 ttggcttgtt ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt
    24181 cagtcctttt ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt
    24241 acttttattc gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg
    24301 cagaccaaaa agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact
    24361 tggcatcaat ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc
    24421 catgttttta atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt
    24481 acctacgggt gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg
    24541 gatgattttt gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc
    24601 atggacaaag ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt
    24661 tttattgaaa gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta
    24721 tttgctagga gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt
    24781 gtcagttgca gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt
    24841 tacttagcat gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt
    24901 tgagggggaa tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa
    24961 aaatcaaggg acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc
    25021 aggtagtgct acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt
    25081 gatccttagc cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg
    25141 gcaacttcat cctaagagaa atccggagac attgctttca atttatggac acacaaaaga
    25201 ttccattcac tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga
    25261 agctgaagaa ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt
    25321 agtatgttac gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg
    25381 tgattcaggt tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc
    25441 agatacctat ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc
    25501 tggagaactt gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt
    25561 tggtgattta gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat
    25621 agaacttgct gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac
    25681 caaatgaaaa ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa
    25741 tgcctttaga tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca
    25801 gaaaccaaaa tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg
    25861 ttgtccgtcg cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta
    25921 gttttatcat gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa
    25981 tcatccaagc acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc
    26041 tagtcattga tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt
    26101 ttgctcatgg aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag
    26161 taaaaaaaga caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata
    26221 tcaaaccatt tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa
    26281 tcttgttgaa aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg
    26341 atgaagattg gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga
    26401 attacgggaa gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga
    26461 ttgaagtcca tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt
    26521 tcttcggact aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata
    26581 aagcatctct atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag
    26641 aaccagaaga attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata
    26701 tggattccgc tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc
    26761 gatcaatgaa agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt
    26821 tcatcatcat taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa
    26881 ataggtttgt atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt
    26941 cgtgtatcga caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa
    27001 aggaatgctc taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt
    27061 cttcgggaag atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt
    27121 aacgcgctaa gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat
    27181 tttttgcttc ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca
    27241 ttaaatcttg cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt
    27301 ggatatgctg tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga
    27361 aatctacttg tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga
    27421 taataacgat tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga
    27481 tacctaatgc cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag
    27541 gtgtacaagc tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag
    27601 tgactaaagt gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg
    27661 caggagtaaa agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa
    27721 aaattccgta ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag
    27781 ctgacgttaa gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa
    27841 taccacttaa aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta
    27901 agagaataag tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa
    27961 aggtccctat agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg
    28021 aataaataaa actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag
    28081 catagttctg ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg
    28141 gtaaatctat cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt
    28201 ggtataaaat cttttcgcct agatatacag taatccattg atggtttcct ttgattagta
    28261 aatttgcttc gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat
    28321 ccacggtagg aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg
    28381 tatagaaaac ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa
    28441 taaaataaaa gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc
    28501 tccactttat acgtaaataa aaagattctt ttttattata tatgttatta aaaaaagaca
    28561 taaaggtatt cctttctaaa acttgtaatg ttagggtaaa cgttttttaa actaacgtca
    28621 attatttata ataaaaaatg agataaagat gaggaaatag aattagaaac atataattat
    28681 taaagggaaa catttttaca gctataattt gaaaaaaatt tatcgttatt atatgattaa
    28741 gaaggattga ataaagaaag aagttgaaaa cgatacctga ttacaaaaaa ataactttta
    28801 taagttctat aacgttgatt atatttttaa ttcttattct tctcgtcgtc ttggttcccc
    28861 gaatttttgg tttgactcct tatattattg aggacaaaga aatgagtcca tattatgcac
    28921 aaggaaatct gatatatgta cgaccgaaaa atacaaatga gattttagtc ggagatatta
    28981 ttacatatta tgaaaattca ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa
    29041 acaaccacga agatttttat acaaaagctg atacaaagac ttatattgag cctggagtag
    29101 taaaaaagag aaatattatc ggtagtccca tctttcagat tccatatatt ggactagtcc
    29161 tttcaaaaac ggcatttgca tggattcagg ggttattctt tacgattgct ttttgcctaa
    29221 caggaatcac agcatggaat acctttgtag agttgaaaaa gaagcggaga cgaacacaga
    29281 aatttttgta agaatgttaa aaacataaat gtattccaga gatccaacga ctcttctgaa
    29341 catgaaacac gagaaaacac tcatgcagct tgtagattta gtcagttgtt cgtataatgt
    29401 gtgaagagga ggagtagtat gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa
    29461 ctattggatt ttatgcgtac ccagcaaaaa aaattaccgg gtgagctagg caaactggaa
    29521 gaagaagctc atgtagcaga agtaccagtt attccccatg aaacagttgt atttttgaaa
    29581 tttttacttg gacagcttca gcctgaacga atcttggaga tcggagcagc tatcggtttt
    29641 tcatcaagtg tcatggccac gacaatcgga gaaaacgctc atgttacgac aattgatcgt
    29701 ttcgatgtca tgatcgagaa agctaaaaaa aactatgaac gtctaggact gacagataaa
    29761 gtgactttgc ttgaaggtca agcagcagat atcttgccgg agttgtctgg tccgtatgac
    29821 tttattttta tggatagtgc aaaatcaaaa tatattgaat ttttacctga atgcttacgt
    29881 ttgttaagaa aaggtggcgt tttgatggta gatgacatct tccaaggagg aacgatcttg
    29941 ctcccagatg aagagattcc ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt
    30001 cttcgtgttg tgatggatca tcccgatttg acatcgacaa tcttgccatt aggtgatggt
    30061 gtgatcttaa tgacaaagga atcagaaaca atcgagttgt gatacatgat tatggcagcc
    30121 tgtagataat cgattgaatc gagggctcaa ttcgaacttg atgtaaaagt ttctatattt
    30181 gtaagtcaat taaaggatac aggaaatcta aataaacagg agtagcatga agtcaatgta
    30241 gctcctgttt tcaattgata accttagaac agctgaagga gaatgtctcg ttttaaaaga
    30301 ttgatttagt agttgggcca aaataattca ttatttgcta attaaataac tatttcgtat
    30361 tgacagaagc aatttaatta tctataatta ttgatgttcc gctgattctt gtcatagtag
    30421 ctcagctgga tagagcatcc gccttctaag cggacggtcg ggggttcgaa tccctcctgt
    30481 gacgctaaaa aaccgttaga aactgaggtt tctaacggtt tttctttgtt ttttgtgcca
    30541 atattgtttg ttttcaaagg caaataaaat aaaaaaacat ccttatcgag tacaattata
    30601 aggagcaaaa aaataaaact catcagaaaa gaggaagaag atatgacgat actatctaga
    30661 attttagtga ctctagttgc aattgaattt ttttatatca tgtatatcga aacggtgaga
    30721 actgattctg acacgacaag tagggttttt aaaatgagta aagaagagtt aagcagaaaa
    30781 tctgttcaaa cattgtttaa gaaccaagga gtgtacaatg gactattagg agtgggacta
    30841 ctttatggag cttatctttc ttccgcatct aaagaaatca caagtatgct attaatatct
    30901 attttttttg ttgcattata tggcagtttg actagcgatc gttggatttt actcaaacaa
    30961 ggtgggctag ctctgttggc attgctttct attttcttat aaatatagaa aaaataagaa
    31021 tttatctaac aggaaacttt gtgaaaaata ttactttatc tcatgaaata tgatattcta
    31081 atgaatggta ttttagaaaa tttcatgaaa aaggagttag ataggtgttc aaaaattatg
    31141 tttcctcact aaaaaaagaa ttttcaggtt atgatctttc aagatttcag aaagatcttt
    31201 tggctgggat cacagtagct gctgtagcat tgcctttagc cttggctttt ggggtatcaa
    31261 gcggagctga tgcggccgca ggtttgatta cggctatcat tgccggtatc gttattggcg
    31321 gtttatcagg cggtttttat caaatatcag gtccgacagg tgcgatggca gctatcttga
    31381 tgtctattgc ggcaacaaag ggaatgcaag gtgtattgct tgctactttt ttagcgggtc
    31441 tatttttact tgcagcagga cttttccgat taggaacact gacttctttt atcccagcac
    31501 ccgtaattac ggggtttact tcaggtattg ctatcattat tgcattaggt cagatcgata
    31561 atttatttgg tgttcactcg gaaggtacaa acgttattga aaaactaagt agttatcaaa
    31621 cgataggatt tccgataaac acagcctctt tgatcatggg agggctagtc attttaggga
    31681 tgtttatcta tccaaaaaaa tgggcgaaac gaatgcctag ttcgctgctg gctatcattt
    31741 tggcaacagc agttatgcta ttgttccatt taccagtagc aactgtgggt aagattccac
    31801 aaacattgat cagtagtaat cgtttgaata tgggagattt tagcttttct gccttgcaac
    31861 aagtggcagt tccagctatt agcattgctt tattaggaat gattgaaagc ttgctatgtg
    31921 gtgcttcggc tggccgaatg gcaaataagc ccttagatag caatcaagaa ttaatggcgc
    31981 aaggaattgg aaatctgttg cttcctttct ttggcgggat tcctgctact gcggccatcg
    32041 cccgtacaag cgtggccatc aaatctggtg cacaaactag attagctggt atgatacatg
    32101 cagttgtctt actgctctcc atgcttgttt ttgcgccagt gatgtcacag attccaatgc
    32161 cagcacttgc aggtgttttg attgtgacgg cttggcgtat gaatgaatgg gaaacgatca
    32221 aggagctatt tactaataaa tattggtcag cagttctatt gtttatcctt acgatgattt
    32281 gtacagttat ctttgattta agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat
    32341 tttttgttgt caaaagcgct gctatcacga tttcattaga aacgatcgat tggcaaagaa
    32401 tggggctgcc agaaacggaa aagctgaata actggacagt agtgtatatt agtggtccac
    32461 tgttttttat gtcttcagaa aaactcaaaa ataaattgga agaactaaat catcacgatg
    32521 gtgtcatttt ctccatgcga ggcgtaccaa gtattgattt gacggcacgt gcagtttttg
    32581 atgaatttca agaaaaagca gagcaaagag gacaaacgat tatctatact tccttgcagc
    32641 cagaagtaga aaaacaactt tctcacttgt gggaacatac attcacgcat caacatcaaa
    32701 cagttgctca tgcgttgaca gctttacatg cgaaatatac tgcaattagc cattaaaaca
    32761 atgaaaaaga acagacaaga ctgtaaactg aagattttat ggtcttgtct gttcttttta
    32821 atattgctgt aaaagtgaag ctagtacttg ccagtcttga tggataacag gtacaaggga
    32881 gcgattgtag aaaatagctg ctggatgaaa aagtggaaga attcgatagt ttttttctga
    32941 ccattcgtag gcatcctttt gttctgttaa ttgtaagaca ggccctgtat agagttgacc
    33001 atgatttttg gtaaccgtat attctttgcc aagtaaacgt tgaagaccaa tgtttccaag
    33061 tgtcgcaatt aattttggct ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc
    33121 aaatacttcg gaacgagtag gtgtccgatt tggataaaca gtttctgact gttgtgtccg
    33181 tttattgatc cgatgagtca cgcgatacgg gcggctcctt acagcactgg tgatatacac
    33241 ctcttctcgt gttaagccag ttgaagaaag tgcctgcatc aattcttttc cagcttgacc
    33301 gctaaaagga acatgcgtat caatctctgt tttccctggg gcttctccca cgagcatcag
    33361 tttaggatga agaggtcctt gcccagcgat aaatccttct acaggacggc cgttcattct
    33421 taaagaagct tcttgtatca gttcttttgg gtattccatt tatgctcctc ctttttcttt
    33481 tattataaga ataaaagaaa cacaatgcga ctattttatt tggtagaact gtgaggatag
    33541 aaaagcagaa gtattgcttg aatttttgtt ttactttttg attttaaatt tagttgacta
    33601 acggttgctg ttctggtaag ctagtactgt tgtaattgtg gactccacag ctacaaccgc
    33661 tcagaacaag taataagcac agcatatgct gtcacttcgg atggcgagtc taagtatatc
    33721 agaaggaggt gctttatagc atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag
    33781 ttgaagtggg acaagcaatc tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg
    33841 tttttgacga agttatctta gtaggcggag aatctacaaa agtaggcgct ccaactgttg
    33901 caggtgcaac tgttgaaggt actgttgaaa aacacggcaa acaaaagaaa gtcgttactt
    33961 tcaaatacaa acctaaaaaa cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa
    34021 aagtggttat cgacgcgatc aacgcttaat agcgtttgag aaagaaggcc tatatatgat
    34081 caaaggaaca tttaagcgaa atgacgcagg tcagatcgtt tcattcactt tgaccgggca
    34141 tgctgatgct ggtccttatg gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat
    34201 cagcactgtc aacgggattg cctcattagc cggttttgag ccgattgttg aaatgaatga
    34261 agaagaaggc ggttatcttt ataccgaagt cacttcagga atgacacaag aacaaaacaa
    34321 tattgctgag attctattag aaaatctttt attaggatta caatcgatag aagctgaaaa
    34381 ctcagaatat atccaaatta aaacaattaa tgataaatag gaggtgcaga ccatgttgtt
    34441 atctatgaat ctacaattat ttgcccacaa aaaaggtggc ggttctacat ctaacggccg
    34501 tgactcagaa tcaaaacgtc taggtgctaa acgtgctgat ggacaaacag ttacaggtgg
    34561 atcaattctt taccgtcaac gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg
    34621 tggagacgac actttatttg caaaagttga tggcgtagta cgtttcgaac gtaaaggccg
    34681 taacaaaaaa caagtgtctg tttatccagt agctcaagaa gcttaattga atcttatgat
    34741 taaagaggtt gtgacaagac ttttgtcaca gccttttttg tttataaaaa taaaagttgt
    34801 agtggaggct acgaagttta ctttgtcttt ttacacttaa tatttttttg ccaatttgga
    34861 atcaggatgt tttgataact atcgttttca attgatggaa gctatattgt ttgctatcag
    34921 tgaaataatt atatggtaaa cagtgttctt tttcttgttt ccattataaa aatcgtatcg
    34981 ggatttttcg ttcttttaaa ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa
    35041 aatcaggtaa atagttgacg aaacagaaat aaatacatag tattatgttt tgaagcctaa
    35101 gtacaatagt ctatagaaaa ggagcgatac cattggcaaa acgcattgac cgaatttata
    35161 tgtatgtcaa agaaaaaacc gctcatttaa caaaggcaga atatgatcaa ggagtaacaa
    35221 cacaggaaat tgctgaagtg ttagggattc agcgaacaaa tagcagtaaa gatctgaatc
    35281 agctagtgcg cgaaggaaaa ctgataaaga cagacggacg accagtacgt tacatatatc
    35341 aaacactcgt tacgcacgta aagccagtta caaaacatgt agtcagttac aaagaaacgc
    35401 cggtctctga agaaaaaaat acactccaaa tagatactaa ggatatcttt gcaaaaatca
    35461 ttggagctaa cggcagcatg aaaaattcag tggaacaggc aaaagctgcg attttgtatc
    35521 cgccaagagg attaaattgt ttgattacag gacctaccgg atcaggaaag acttattttg
    35581 cacacgcgat gtatcatttt gcaaaagcgc atcatgtaat agatgaggaa acggaactga
    35641 ttgttttcaa ttgtgctgat tatgccaata accctgagct tttgatgagt catttatttg
    35701 ggtacgcaaa aggtgctttt acaggcgcag atgaagaaaa aatggggatt atcgatcaag
    35761 cggatggcgg tatgctgttt ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa
    35821 tgatctttta ctttatggat catggaactt acagtcgtct tggggaaaca acgaaatccc
    35881 atgaagcaaa tgtacggatc gtaggtgcga ccacagaaga cccaggttct tctctattag
    35941 aaacttttgt tcggcgtatc ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg
    36001 caaatgagaa agtcgattta gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc
    36061 gaaagatttc tttgacagaa gatgtagcaa aagcattgat cggcagtgtg acctatggaa
    36121 atataggaca attgaagtca aatatccagt tagtatgtgc acgaggattt ctaaatcata
    36181 tgcaatcacc cgaaatctcg atcacaatcg atgatttgac agaagggatt cgtagcggat
    36241 tgattcaatt agcaagcaac cgcagtgcaa tggcagagct ttctaaaata ttggagccaa
    36301 agataactgt agcaccaaat gatacgatca tgaaaatcca atcggactcc tatgagttac
    36361 cttataatct atatgatatc atcggagata aagcagcttt actgaagtcg gatggtttgg
    36421 atcaagaagc aatcaatcat ttcatttcta cagatatcaa catccatctg aagtctttct
    36481 ataaagacca tggtttttct tttaatgcag acagcaaatt agcagaattt gtagatccga
    36541 aaatcataga agtgaccaat cagatttatg taatggtcaa aagatcttta ccatatgagt
    36601 ttcagcaaaa ctttatttat gctatgagct tgcatatcag ttcttttttg aaaaaaatca
    36661 acttaggaga agaacggcat acgaatgata acatccgtga gatggctttt gattttccag
    36721 atgaatatgc tgttgcgaaa gaagtgcgta gttatatcga aacgtatttt caagtggaga
    36781 ttccagaaag tgaagactat tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag
    36841 caagcggcag aatcggtgta gtcgttgcag ctcacggaaa cagcacagca agcagtatgg
    36901 tccaagttgt ccaacagttg ttagatgcag agaacgtacg agcagtagac atgcctttgg
    36961 atatggatcc gaaaacagca ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag
    37021 gaagcggggt catactctta gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc
    37081 aaagagacac tggaattcca gtacgtactg ttgatatggt aacaacttct ctagttttgg
    37141 aaactgtacg aaaggtctca gtattaggga cagatctaga tatgttgcat gaatcattga
    37201 aaaattttcg aggttacgca gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta
    37261 ttcataagaa aaaagctgtt ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa
    37321 tcaaagaatt gatcgaacga gcggtaaaca agcgtcaaga aacagaatta gaagttctag
    37381 ctttgtcgat tgtggaagtg aaagaagaac tgccagttat ccaaaaagaa tatcagatca
    37441 tagctactac tgggattact gatttgaata tccaagcccc atttattcct ttagaacgtt
    37501 ttattgatca aaatatcgaa gtcattttgg atcagttgct gatggaatca gaattggagg
    37561 aaactgaatt catctcccta gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct
    37621 ctgataactt catctttatt aatggtagta agttaatcga tccaatgtgg caattttcta
    37681 cacaaatctc tcagacgacg ggaatcggcg atgaagaata tggattcaaa atcaacctag
    37741 tgatgcacac agcaggaatg atcgaacgta ttattcggaa tgaaccattg actgtggaag
    37801 aaaacgaatt gacaaataca acgaatgatc ctctttatag ccaattggca gcctcagttg
    37861 ttctgttgga agatcagata aaagtaaaag tgccgataga agaaatgtat tatctgctac
    37921 gtctagtcca taatcagtta gataaaaaag aatacactgt gccatgaata cacaaaaacg
    37981 atacactttt atagtgtatc gtttttgtgt attggttaag aatcctttaa attcaattag
    38041 tgtatcgtaa aaaagttggc acgctttttg cttatagtag tgtgtgctag gaggtggttg
    38101 ttcacacaga aatgataggg gtttctgtga agtagtgagg aagaaggagt tttgcagatg
    38161 gatattcgat tggcacgtat agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg
    38221 tcgaagcaaa caggtgttga acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa
    38281 ttgagaaagt ttctactaaa agaagctgca ccgccgggga tcacagcaaa tgtaataacc
    38341 gtgcagaaaa tgattcaaat ctatcatagt ggattattat cacgagcaaa agtgatgctt
    38401 ttattcacca atccccaaga tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg
    38461 ttgaatatcg gcggtatgag ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca
    38521 gtaaatcaac aagatattga ttcctttcgt tacttgaaca gtcaagaagt ggagttagag
    38581 attcgaaaag tacctgctga caggaaaatc cttttgatgg aactactagc gaaaatcaaa
    38641 aaggtgtagc aagaatggtt gttggaaaag gtcaaataat tctgttgtaa tggttagcaa
    38701 ttattaatat ttttgaattt gttacaacaa aagtaaatag gaggtataaa tatggtaggg
    38761 attatcctag caagtcatgg acaatttgct gaaggaatct tacaatccgg ttcaatgatc
    38821 tttggcgaac aagaaaacgt caaagccgtg attttgaaac caagtgaagg tccagatgac
    38881 ttgagagcca aattggaaga agcggttgct tcttttgata atcaagacga agtgttattc
    38941 ttagttgatt tatggggcgg aacaccgttc aatcaatcaa acacattatt cgaagagcac
    39001 aaagataaat gggcaatcgt cagcggtttg aatttaccaa tgttgattga agcctacgca
    39061 tcacgctttt caatggaatc tgctcatgaa attgcagcac acatcattga aacagcaaaa
    39121 gacggtgtaa aagtaaaacc ggaagaatta gaaccggcag aagcaccgaa agcagcagtt
    39181 gaagacgcac aacctaaagg agcacttcca gaaggaactg tggtcggaga cggaaaaatc
    39241 aaatatgtat tagcacgtgt tgactcacgt ctgttacatg gacaagttgc gacagcttgg
    39301 acaaaagcag tgcaaccaaa ccgtattatc gttgtttcag atgcagtatc aaaggatgat
    39361 ctgcgtaaga gactaatcga acaagctgct cctccaggtg taaaagcaaa tgttattcct
    39421 attagcaaaa tgatcgaagt agcaaaagac cctcgttttg gaaataccaa agcattgctt
    39481 ttatttgaaa atcctgaaga cgtattgaca gctgttgaag gtggcgtaga catcaaagaa
    39541 ttaaatgttg ggtcaatggc gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct
    39601 atgggacaag aagacgttga agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat
    39661 gtacgtaaag tacctaatga ttcacgcgat aacatggatg atattttgaa aaaagcaaaa
    39721 gctgaattag caaaagcgta ataaataata ttaaaattta taggaggctt atcatgtcta
    39781 ttatttcaat aattttagtc gtattagttg cctttctagc tggtatggaa ggtattcttg
    39841 atgaatttca atttcatcaa ccactagtgg catgtacatt gatcggttta gttaccggta
    39901 acctcgaagc tggtatcgta cttggcggta cgcttcagat gatcgcttta ggttgggcaa
    39961 atatcggtgc cgctgtagca ccagatgctg cattagcatc agtagcatca gcaattattt
    40021 tagttttagg tggacaaggt gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt
    40081 tggctgttgc agggttgttc ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc
    40141 atttgatgga tgcggctgct gaagaaggaa acatccgtaa ggttgaaatg tggcacatca
    40201 ttgctgtatg tttacaaggg gttcgtatcg cggtcccagc agcagcgttg ctatttatcc
    40261 cagcacaaac tgtgcaatca ttccttgaat caatgccagc atggttaaca gatggtatgg
    40321 ctatcggtgg gggaatggtc gtagctgtag gttacgcttt ggtaatcaac atgatggcta
    40381 caaaagaagt atggccattc ttcgttattg gtttcgttgt agctgcaatt tctcaattaa
    40441 cattgattgc tctaggtgct ttaggtgttg cattagctct tatttacttg aacctttcta
    40501 aaatgggtgg ttcatcaaat aatggaggcg gaagcaactc tggtgaccct ttaggcgaca
    40561 ttttgaatga ctattaatct tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt
    40621 atcaaaaaaa gatcgtttag ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa
    40681 ctacgaacgt atgcaaaacg gtggctgggc attttctatg attccagcaa tcaaaaaatt
    40741 atacaaaaca aaagaagaac gttctgcagc attaaaacgt cacttggaat tctttaatac
    40801 acacccatac attgcttctc caattttagg ggttacattg gctctggaag aagaacgtgc
    40861 gaatggcgcg cctgttgatg atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc
    40921 tttagctggt gttggtgacc cagtgttctg gtttacagta cgtccaatgc taggtgcact
    40981 aggtgcttca ttagctatgg gaggaaatat ccttggacca atcatcttct tcttaggttg
    41041 gaacattatt cgttgggcat tcatgtggta tacacaagaa tttggctaca aagctggttc
    41101 taaaattact gatgaccttt ctggcggatt gttacaagac gtaacaaaag gtgcttcaat
    41161 tttagggatg ttcgtattag ctgcccttgt acaaagatgg gtatcaatca aattcttacc
    41221 agttgtatca actgtaaaat tagataaagg tgcttacatc gaatgggata aattgccttc
    41281 aggtggtgaa ggaatcaaga gtgcatttga acaagttaat aatggcttag ccctttctcc
    41341 tgaaaaggta acaacattac aagacaactt agaccaattg attcctggtt tagctgcatt
    41401 aggattaaca ttgttatgta tgtggttgct taagaaaaag gtttctccaa tcgttatcat
    41461 ccttggttta ttcgtagtcg gtgttgttgg acacgtcatc ggacttttat aatccataaa
    41521 aatatagctc gggcgttgtc ccgagctttt tttatatagt atactgttca taaacaaata
    41581 tctggaggga gaaaatggtt caatcaatca atacaaaagt agacttggta attgatgcta
    41641 cttcgtttac gggtctgaca gattacgggc aaatcatgat aggtgataat ggttttgagt
    41701 tttaccatgc gcgagataga cgcaaatata tccaaattcc atgggaagaa gtggattatg
    41761 tactagcatc agtcatgcta aagggaaaat ggattcctcg atatgccatt caaacgaaaa
    41821 aaaatggtac atacacattt tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag
    41881 aatatgtaga tgaagataga atggtacaat cattgagttt ctttgatgta gtgaaacgtg
    41941 gtattcgtag tatgtttaaa cgcaagtaaa aaataaagaa aacttctaag aggaggtttt
    42001 ctttattttt ttactttgca gaagaaaata tgaaatagca aaaccaatta tttgtcccct
    42061 ttaaatccca tatgctaaaa ttgattagca aagaaggagg ttatattatg agttggttat
    42121 ggacattgat tgttggagcc gtgatcggag caattgcagg tgcgatcacc agtaaaggaa
    42181 attcgatggg ctgtgtaacg aatatcattg ctggactagt cggttcaagt attggacaag
    42241 cgattttcgg cgaatgggga ccaaaagctg caggtatggc acttattcca tctattttag
    42301 gcgcagtgat tttggtagca gttgtctcat ttttctttgg aaaacgatca taagttttta
    42361 caaaatagta aactaaaagg agttgcagcg agactgtgac tcctttttgg ctttatttat
    42421 ttaaaataaa aagaagtttt ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt
    42481 tgctataatg gaaaaaagcg tgcaaagaaa gagggagttt cattatgatg ctacgtgtag
    42541 aaaaattgcg caaaaaaatg caagaagaaa atttggattc atttttggtt accagtccgt
    42601 acaatcttcg ttacttaacg aactttacag gtacgacagg attagctgtt attactttgg
    42661 aaaaagcatt ttttattact gattttcgtt ataccgagca ggcggcagcg caggctcaag
    42721 ggtttgaaat tattaaaaat gtcggaccta tttttgatga ggtagcaaac ttagtacgca
    42781 aagaagaact gcactcatta gcttttgagg aaacaacagt ctcattttta gaatattcgg
    42841 ttttagaaga aatcatcgat gcggaattag tacctgtttc acagatgatc gaagaactca
    42901 gagaagtgaa agatgaagaa gaaatcgcta ttatcgaaaa agcatgtcat attgcggata
    42961 tggcatatga tcacatcctg aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa
    43021 atcaactaga cttttatatg cgttctcttg gcgcttcagg tgtttctttt gatacgatcg
    43081 ttgctagcgg attgcgttcg gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac
    43141 aaggtgactt gatcacattg gactttggtt gctattacga agggtacgtt tccgatatga
    43201 cacgtacatt cgctattggt gatcctgggg aaaaattaaa agaaatttat caaatagtct
    43261 tagatgctca attagcagtt attgatgctg caaaaccagg aatgactggt gtccaattag
    43321 atgcagttgc tagagattat atttcaaaac atggttacgg ggaagcattt ggacattcta
    43381 caggacatgg gatcggttta gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa
    43441 agcaatttgt accaggaaat gtgatcactg atgaaccagg gatctactta ccaggaatcg
    43501 gtggagtacg tattgaggat gacttgttga tcacagaaga tggaaatcgt gtgttgactc
    43561 gttcaccaaa agaattgatc atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat
    43621 cagtgttcac agactttgtt atagaaaatc actcgattta atgatacact atagaagaac
    43681 ttttaagctg taagagataa taaagttggg tgttttctcc aattcatagc agtttgcagc
    43741 tgatcggcgg cagttttctt accacgtgat cactatgccg catcatagag aattcggatg
    43801 tatctcgtgc ttaggcaaag ggaggaataa ataaatggca gatgaaaaaa atttgatttt
    43861 aaacgcaaac caagatttag gagagatcgt gatcgctcct gaagtcatcg aagtaattat
    43921 cgggattgca gcttctaaag tagaaggcgt atacggaatg cgcggaactt ttgccagcaa
    43981 tgtcactgaa ttattaggac gtgcagctca cggaaaaggt gtttacttag caaacgagga
    44041 agatggcttg aaagtagatc tttactgtta tctagaatat ggtgtttcag ttcctaaagt
    44101 agcaatggaa atgcaggaac gggtcagcca gcaagtcctt ttcatgacgg atatcgattt
    44161 atctgaagtc aatatccatg tagtagctgt tgttcctgaa aaattagaac aaccaacaat
    44221 cgaagaactt tttgaagacg aagaggaaga gaatgaataa agaattatca agacacgaga
    44281 ttcgggaaat ggcattgcag gctttatttc cattagactt caatgcagac ctaacaaaag
    44341 aagatgcgat tttcaacgca atcgaattag accaccgcga catgatcaac gaagatgaat
    44401 cagagtttgt tcctgtttat cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg
    44461 aattggataa agtgattgaa aaacatttaa aaaacaattg gcacatcaat cggatctcaa
    44521 aaatggattt ggtgatcttg cgtatcgcga tttttgaaat gacttatgtg acagatgtac
    44581 cagcggctgt tgctttaaat gaagcaatcg agttggcaaa aacatttagt gatgaccgtt
    44641 cacgtaaatt cgtcaacggt gttttatcca atgtattgaa agaatta
//
LOCUS       DACCDF010000015        43653 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCDF010000015 DACCDF010000000
VERSION     DACCDF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.015"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            117..971
                     /locus_tag="IVK51_000797"
     CDS             117..971
                     /locus_tag="IVK51_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP6430410.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            972..2309
                     /locus_tag="IVK51_000798"
     CDS             972..2309
                     /locus_tag="IVK51_000798"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP6430411.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            2314..2538
                     /locus_tag="IVK51_000799"
     CDS             2314..2538
                     /locus_tag="IVK51_000799"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP6430412.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            2540..3430
                     /locus_tag="IVK51_000800"
     CDS             2540..3430
                     /locus_tag="IVK51_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6430413.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            3439..4257
                     /locus_tag="IVK51_000801"
     CDS             3439..4257
                     /locus_tag="IVK51_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP6430414.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            4364..4813
                     /locus_tag="IVK51_000802"
     CDS             4364..4813
                     /locus_tag="IVK51_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6430415.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            4831..6513
                     /gene="recN"
                     /locus_tag="IVK51_000803"
     CDS             4831..6513
                     /gene="recN"
                     /locus_tag="IVK51_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP6430416.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(6568..6684)
                     /locus_tag="IVK51_000804"
     CDS             complement(6568..6684)
                     /locus_tag="IVK51_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP6430417.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(6757..7713)
                     /locus_tag="IVK51_000805"
     CDS             complement(6757..7713)
                     /locus_tag="IVK51_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6430418.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            7884..8252
                     /locus_tag="IVK51_000806"
     CDS             7884..8252
                     /locus_tag="IVK51_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP6430419.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            8422..8853
                     /gene="mraZ"
                     /locus_tag="IVK51_000807"
     CDS             8422..8853
                     /gene="mraZ"
                     /locus_tag="IVK51_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP6430420.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            8874..9833
                     /gene="rsmH"
                     /locus_tag="IVK51_000808"
     CDS             8874..9833
                     /gene="rsmH"
                     /locus_tag="IVK51_000808"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP6430421.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIGLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            9838..10239
                     /gene="ftsL"
                     /locus_tag="IVK51_000809"
     CDS             9838..10239
                     /gene="ftsL"
                     /locus_tag="IVK51_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP6430422.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            10240..12432
                     /locus_tag="IVK51_000810"
     CDS             10240..12432
                     /locus_tag="IVK51_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP6430423.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            12458..13420
                     /locus_tag="IVK51_000811"
     CDS             12458..13420
                     /locus_tag="IVK51_000811"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP6430424.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            13434..14795
                     /locus_tag="IVK51_000812"
     CDS             13434..14795
                     /locus_tag="IVK51_000812"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP6430425.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            14821..15909
                     /gene="murG"
                     /locus_tag="IVK51_000813"
     CDS             14821..15909
                     /gene="murG"
                     /locus_tag="IVK51_000813"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP6430426.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            15924..17144
                     /locus_tag="IVK51_000814"
     CDS             15924..17144
                     /locus_tag="IVK51_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP6430427.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            17300..18625
                     /gene="ftsA"
                     /locus_tag="IVK51_000815"
     CDS             17300..18625
                     /gene="ftsA"
                     /locus_tag="IVK51_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP6430428.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            18648..19889
                     /gene="ftsZ"
                     /locus_tag="IVK51_000816"
     CDS             18648..19889
                     /gene="ftsZ"
                     /locus_tag="IVK51_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP6430429.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            19906..20583
                     /locus_tag="IVK51_000817"
     CDS             19906..20583
                     /locus_tag="IVK51_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP6430430.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            20597..21196
                     /locus_tag="IVK51_000818"
     CDS             20597..21196
                     /locus_tag="IVK51_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP6430431.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            21211..21498
                     /locus_tag="IVK51_000819"
     CDS             21211..21498
                     /locus_tag="IVK51_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP6430432.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            21547..22329
                     /locus_tag="IVK51_000820"
     CDS             21547..22329
                     /locus_tag="IVK51_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP6430433.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            22350..23057
                     /locus_tag="IVK51_000821"
     CDS             22350..23057
                     /locus_tag="IVK51_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP6430434.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            23319..26117
                     /gene="ileS"
                     /locus_tag="IVK51_000822"
     CDS             23319..26117
                     /gene="ileS"
                     /locus_tag="IVK51_000822"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP6430435.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(26166..27692)
                     /locus_tag="IVK51_000823"
     CDS             complement(26166..27692)
                     /locus_tag="IVK51_000823"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP6430436.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(27707..28354)
                     /locus_tag="IVK51_000824"
     CDS             complement(27707..28354)
                     /locus_tag="IVK51_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP6430437.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(28538..28867)
                     /locus_tag="IVK51_000825"
     CDS             complement(28538..28867)
                     /locus_tag="IVK51_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP6430438.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            29044..29772
                     /gene="sfsA"
                     /locus_tag="IVK51_000826"
     CDS             29044..29772
                     /gene="sfsA"
                     /locus_tag="IVK51_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP6430439.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            29788..30801
                     /locus_tag="IVK51_000827"
     CDS             29788..30801
                     /locus_tag="IVK51_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6430440.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            30801..32078
                     /locus_tag="IVK51_000828"
     CDS             30801..32078
                     /locus_tag="IVK51_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6430441.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            32141..34843
                     /locus_tag="IVK51_000829"
     CDS             32141..34843
                     /locus_tag="IVK51_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6430442.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            34995..35312
                     /locus_tag="IVK51_000830"
     CDS             34995..35312
                     /locus_tag="IVK51_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6430443.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            35342..35662
                     /locus_tag="IVK51_000831"
     CDS             35342..35662
                     /locus_tag="IVK51_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6430444.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            35748..37208
                     /locus_tag="IVK51_000832"
     CDS             35748..37208
                     /locus_tag="IVK51_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6430445.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            37276..37497
                     /locus_tag="IVK51_000833"
     CDS             37276..37497
                     /locus_tag="IVK51_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430446.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            37528..37710
                     /locus_tag="IVK51_000834"
     CDS             37528..37710
                     /locus_tag="IVK51_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP6430447.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            37710..38123
                     /locus_tag="IVK51_000835"
     CDS             37710..38123
                     /locus_tag="IVK51_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP6430448.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            38246..39427
                     /locus_tag="IVK51_000836"
     CDS             38246..39427
                     /locus_tag="IVK51_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430449.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            39816..39889
                     /locus_tag="IVK51_000837"
     tRNA            39816..39889
                     /locus_tag="IVK51_000837"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39850..39852,aa:Arg,seq:cct)
     gene            complement(39958..41097)
                     /locus_tag="IVK51_000838"
     CDS             complement(39958..41097)
                     /locus_tag="IVK51_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6430450.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(41396..42031)
                     /locus_tag="IVK51_000839"
     CDS             complement(41396..42031)
                     /locus_tag="IVK51_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430451.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(42144..42779)
                     /locus_tag="IVK51_000840"
     CDS             complement(42144..42779)
                     /locus_tag="IVK51_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP6430452.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(42813..43274)
                     /locus_tag="IVK51_000841"
     CDS             complement(42813..43274)
                     /locus_tag="IVK51_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP6430453.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(43404..>43653)
                     /locus_tag="IVK51_000842"
     CDS             complement(43404..>43653)
                     /locus_tag="IVK51_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP6430454.1"
                     /translation="FEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDKSQF
                     NLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT        14435 a         7418 c         9117 g        12683 t
ORIGIN      
        1 aattagaagc tggggcgtaa gtcctagctt ttttctatga atcatcataa acctgttata
       61 aatcgggaaa attctatggt agaatgaaaa cacaaaaaat atctgggagt ggttagatgg
      121 cagaattgat taacggaaaa gaattagctg aaaaaatgca ggcagagatt gctgaaaaaa
      181 tccaaaaatt gaaagataat ggtatccatc ccggattagt cgttttattg gtaggggaaa
      241 atccagccag ccaaatctat gtaagaaata aagaacgtgt agcaaaagaa atcggaatcc
      301 attcattggt agagcgttat cctgagacga tttctgaaga cgaactactt gcagaaattg
      361 aaaaatacaa tcaagatcct agttttcatg gtatattggt ccaattacca ttgccaaagc
      421 atatagatga agaaaagatc ctgttagcta tccgccctga aaaagacgta gacggttttc
      481 atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga caagattcct tgtactccat
      541 acggcatcat gaaaatgttt gaagcttatt ctatcgatcc ggcgggtaaa cgagcagtag
      601 ttatcggacg aagcaatatc gtcggtaaac cgatggctca gctactcttg atggcagatg
      661 caactgttac tgttgctcat tctaaaaccg aaaatctggc tgaactggca caagaagcag
      721 atattttagt cgtagcaata ggacgtggac attttgtcac aaaagaattc gtaaaaccag
      781 gtgctgtagt gatagatgta gggatgaatc gtgatgctaa cggtaagctg atcggtgatg
      841 tgaaatttga tgaagtggag cctattgcag gctggatcac tcctgtgcca aagggtgtag
      901 ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa atctgccgaa gcttttggag
      961 caggtgaata gatggcacaa gaatatttaa ctgtcactac attaacgaag tatttgaaaa
     1021 gaaaatttga tgccgatcct tatttagaac gcgtatattt aacaggcgaa atatcgaatt
     1081 tccgtttacg tgccaatgcc catcaatatt tcagcttaaa agatgaccat gcaaaaatat
     1141 ctgctattat gttcaaagga gcattccaaa aattacgatt ccagccaaaa gaaggaatga
     1201 aagtccttgt cgttgggcgt atttcattat atgaagcggg aggttcttat caaatctatg
     1261 tagagcacat ggagcctgat ggaataggtg ctttatatca agaattggaa gaacgaaaag
     1321 aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa aatgctgcca cgtttcccaa
     1381 agcgaatcgc ggtccttacc agtccaagcg gagcggttat ccgcgatatc atcacgacgg
     1441 tgaaaagaag atatccaatt gtccaattgg ttttgttccc aacagttgta caaggaaacc
     1501 aagcagcgga caatgttgtc aggaatatcc agagagtaga agagatgggc aatttcgata
     1561 caatgatcat cggtcgtggg ggtggttcga ttgaggattt atggccgttt aatgaagagc
     1621 gggttgctcg ggcgatttat caagctaata cgccggtgat ttcatctgta ggacatgaaa
     1681 cagataccac gatcgctgat ttagtagcag atgttcgggc tgccactcca acagcagctg
     1741 cagaattagc tgttcctgtt ttgtcagaag agttgatgcg cattcaagaa cggcaagcta
     1801 gattgcagca ggcgtttgta cgccagatcc agcgcaaaca agaacggttc gagcgtgctc
     1861 gacgttccta tgtatttcgg cagcctgaaa gattgtatga aggtcagacc atcaaattag
     1921 accagttgaa acagcgatta tatcaagcta cacagcaaat ttatcatgcg aaagaaaaac
     1981 aaacgatcgc attaagccac caattggaac aagtggctcc gctttatcga gttaaagcag
     2041 ctcgccaaga aactgcttat ctagaaaaac gcttagaaga gaaaatccaa caatatatga
     2101 aacaacagca gcagcgtttt gctcaagctg ttcaatctct tgatttgttg agtccattga
     2161 aaatcatggg aagaggctac agctatacga cgttaaatga tcaagtagta aaaagtgtca
     2221 ccgagattca tcaaggggat ttgctgacga tccattatca agatggaatc gttaaaggac
     2281 aagtacaaga gatagacaaa ggagaataaa aatatggaaa atccaacttt tgaagagtca
     2341 ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag gagacgttcc attagaatca
     2401 gctctagatt cgtttaaacg aggaatggaa ttaagcaaac attgccaaga tacattagct
     2461 aaagcagaag agaccttgac gaaaatgatg acagaatcaa atgaagaagt tgattttgac
     2521 ggaaacgagg aaagctgaga tgagattaac tgacttttca gctattcatc tacctgctgt
     2581 agaaaaagaa atcttgtctt ttttagatga gcatacaacg gatagaggac tctatgatgc
     2641 gatgagttat tcagtgaaag caggcgggaa aagaatacgt cccttattac ttttaacggc
     2701 cgttgcttcc tttgatgagc ctattgatgt tcccgtctat caagtagcag ctgctttaga
     2761 aatggtgcat acgtattcct tgatccacga tgatttgcct gcgatggaca atgatgatct
     2821 tcgtagagga aaacctacca atcataaagt attcggcgaa gcattagcta tattggccgg
     2881 agatggtttg cttacaggag cattccagct aatcagtatg gcacacttag gtaattctcc
     2941 taagcttctg ctgcttcagc aattagctgt ttgtgcggga tcacaaggta tggttgctgg
     3001 gcaagctgca gatatcgagg gcgaatcgaa aaagctttct ttagaagaat tagcatttat
     3061 tcatgaaaga aagacagggc atttgattcg ttatgccttg ctggctggtg gcatattagc
     3121 gaagcaacct gaagaaatct tattgctact tcaacgatta gccgaacatt taggtttagc
     3181 ttttcagata agagatgatc tactcgatgt gattggtaca acgaaaacat tagggaaaac
     3241 agctggtaaa gatgaacgta tggaaaaaaa tacttacccg cgtttattag gcctagaaaa
     3301 gacacgtgag gcgttggaga ttgagctgtt atccgccaac aagatcatag acaaattaga
     3361 agaggacgtt ttttcttttg atggcgggct gatgcgccag atgatcaagc aatttgatgt
     3421 ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg tattagcggt caaacaagga
     3481 ttatttgaaa caagagaaca agccaaacgt tcagtgatgg ctggtttgat ttacaacgaa
     3541 aaaaacgagc ggtttgataa accgggagaa aaaattccag tgagtagtga attaagagtc
     3601 aaaggtaaaa aacttcctta tgtttctcga gggggattaa aattagaaaa agcactcaaa
     3661 gcatttgatc tatctgttga aggaaaaaca ttgttagata ttggtgcatc cactggcgga
     3721 tttactgatg cagctttgca aaatggcgca aaaatgagtt atgctttaga cgttggctac
     3781 aatcagctag cttggaaaat aagacaagat cctagagtcg tcgtaatgga aagagtgaat
     3841 tttcgttatg caaaacccga agactttacc ttaggaattc cagaagttgc tgttatcgat
     3901 gtttctttta tttcactaaa actgatgctg cctccattac atgctatttt aaaagaagag
     3961 ggagaagtga ttgctttaat caaaccccaa ttcgaagcag gcagagaagc tgtagggaaa
     4021 aacggaattg ttcgtgaccc tcagacccat caaaaagttt tagaagacat tttgtcattt
     4081 gcggcacaag gtggatatga tgtgctggaa ttaagttatt ctccgatcac ggggggagaa
     4141 ggaaatatcg agttcttggc tcacttgagg aaagtacccg aaagcggaac aatcaattca
     4201 gctattaata tggcagaagt ggtttcaaat gcacacgaac agtttgacca taaataaatt
     4261 gtaactgtga aaatcaagtg agaagtatgc ggattcttct ttattatacc aaggaaataa
     4321 gctatcattg attaagaaga atatgctaag gagcgaagac gtaatgagaa aaaaagatcg
     4381 gcatcgtttg attactcgat tgttgagtga gcaagatatc cgaaagcagg aagaatttgt
     4441 tgaaatcctg aaaagcaaag gaatatctgt tactcaagct acgatttcca gagatataaa
     4501 ggaactgaaa ctgatcaagg taccggcttc agatggcggc taccgttata gcataccagc
     4561 tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta ttaaaagacg ctttcgtggc
     4621 agttgaccaa atggaaaaat ttgttatttt gaaaacattg ccgggaaatg cttcggcagc
     4681 agcaaatttg attgataaac gttacaagaa agaattattt tcgattatca atgatgacga
     4741 taatgttttg atgattacac gaactgaaga ccaggcagtt ttattgaaga aagacttcct
     4801 gcattacctt taagttgagg agcgaatgtg atgctacaag aaatcagtat tactaacttt
     4861 gcgattattc cggaattgcg gctttctttt cacgaaggaa tgaccgcgct gaccggagag
     4921 actggtgcgg gtaaatccat tattatcgat gctttaggtc tgttagcagg aggaagaggt
     4981 tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc ttgaagggtt gtttgaacta
     5041 ccaaaacaag aaggattctc tgaactgatg gttgaattag ggatcgaaac agatgaggac
     5101 aatctgattg ttcgacggga tatgtcctta actggaaaaa atgtttgtcg agtgaatggt
     5161 catattatca cattagctaa tctgagaaga atcggcagtt atttagtaga tatccaagga
     5221 caaaacgaac atcaggaact gctccagcct gaatcacatc ttgctttatt ggatcgtttt
     5281 ggagatgtag ctttccaaca gaagaaaaag agttatcagc aagaatatat ctcttatcgt
     5341 gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa aatcctatgt tcagagaata
     5401 gatatgctcc atttccagca agaagaaata gctgctgctg atttgcaagt cggagaagaa
     5461 gagaaattaa aagaagaaag agaaaaactt agcaattatc aaaaaattgc tgatggatta
     5521 gctgcaggtt acggagcact gacgaataat gaacaaaaca gtgtagatgg tgttggcttg
     5581 gccgtttcag aactgcaagg gatcgcccat ctagatgtgg agtatgaagc aatttatgaa
     5641 aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg gcgatatgag tcgtcagatt
     5701 gatttattgg agttagatga gagtcgttta gaagaagtta cgcaacgttt agaattaatc
     5761 cgacagttga aacgtaaata cggcgaatcc attgagtcaa tattggctta ctatgacgaa
     5821 atcaccgaag agctagcttc ttctgatttt tcagaaggtc aattggacaa aatgaaagag
     5881 gaactagagc aaaaagagct tctacttcag caacaagccg cagatcttca tgaagcgcgt
     5941 aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg aattgaaaag tctgtatatg
     6001 gaaaacactg agtttgaggt acgtttctta aaagaagaaa atagacaatt gaatcatgat
     6061 ggatttgacc aaatcgaatt ttacattacg accaatccag gagagccact gaagcctttg
     6121 gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc ttgctttgaa aactattttt
     6181 tcttctgagc aaggtgtgac aagtattatt tttgatgaag tggatacagg tgtgagcgga
     6241 agagttgcac aggccatcgc agataaaatt tctcaaatat cgaaatactc tcaagttttg
     6301 tgcatcactc atctgcctca agttgctgca gtcgccgact atcaatatta tattgtgaaa
     6361 gcagtgattg atggacgtac gcaaacttct gtttcagagt tgaaaacaaa agaaagagaa
     6421 gaagaaattg cacgtatgct ggctggaagt gagattacaa aactcacact cgaacatgca
     6481 aaagaactat tgaaactcgc aaaaaggacc tgagccacct cgcagtggtt caggtccttt
     6541 tcagcagctg atagctatat tttattttta catgacactc attacagcag tcaatacaac
     6601 agatccaatc gttaaaatca gcattgtcca aatgacaaat tttgttactt tagcaaaagt
     6661 acttgtttgt tttttttcac gcattgtttt caacctcgtc attaattagt tgattcaagt
     6721 gtacaatctt ttttccattt ccacaagagg gcttttttat aaaaacttcc ctttttttaa
     6781 ataataggta gttacgatac aaataaataa tgttaataga actagccata taaaggcatc
     6841 atccctttca gcaataggta acttaatgtt cattccatag atactgccga cgattgtcgg
     6901 tatagttaaa ataatcgtta aagaagttaa aattttcata atattattca gattattcga
     6961 tacgattgct gaaaaagtct gattcatttg ttctactagt tttaattgaa tgcgagaagt
     7021 agtcattgcc tgctctgttt ccaccaatac atcacgtaaa tgatttttca aagcatgatt
     7081 ttcttggaaa agtttatttt gcgctagttc tttcaatgca tctaaattag cagaagtcgc
     7141 ttctctgaaa taaacgaggc ttttttggat atccatgatt tgataaagtt gtttgttttc
     7201 agtggatact tgaagtttgg cttccaaagt atcgcattga gattgaagcg tagctaaata
     7261 actgttgtaa gagagtgtga cctgccaaat gaattgaaag aagatattga gattaggcga
     7321 aatatcactt tttggaaagg tctggctaaa gactttcttt aaaaacagtg gttcatgatt
     7381 cgtaattgtg ataagcttct ttttgggcgt caaaataata gagagcgggt atgtatccac
     7441 ttgaaaataa ccgctaggac ttgttttagc aaaaggatat tgtaacaata ataacgctgg
     7501 ctttgtcaac tttgattgat tgaattcttc tgtacgggaa ttttctgcat cgtccaatat
     7561 ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc agctgatgaa tctcttcttg
     7621 attaggacgt tctacacaat accagatcgt ttggtcagtt tcctttccgg tattacgcag
     7681 tttctcatta tctaatgtaa aatatttgat catgattttc ctcctgttgc ctgtttcttt
     7741 tattataacg gatagggaat tggaatacat ggaatgtgtt tgtaagatat agaatataac
     7801 tgcataaatt taaataaaat gaaacagttg tgtaaagtat atacagtata cttgaagcaa
     7861 cgataaaaaa ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata
     7921 tttacccggt aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc
     7981 gtcgttttaa aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg
     8041 cattgtctag aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc
     8101 taatgggaat ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc
     8161 gttatttcaa gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct
     8221 tgatcattgt gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt
     8281 ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc
     8341 ccggatttcc cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag
     8401 tggaggaatg gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa
     8461 ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca
     8521 cgcggattgg atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct
     8581 aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat
     8641 tcggctgcca ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta
     8701 agaaattatg cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa
     8761 atttgggatg agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa
     8821 attgctgaaa gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg
     8881 aaacatttca acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac
     8941 cggatggcgt ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa
     9001 gccaactaag ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc
     9061 acgccaagct gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga
     9121 acttcagaga attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt
     9181 atgatctagg ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc
     9241 aagatgcacc gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa
     9301 ttaatgaata ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat
     9361 tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa
     9421 ctggtgagct tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg
     9481 ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg
     9541 ccgaagaggc ctctttagaa caggcaatcg ggttattgaa agtcaatgga agaatcagcg
     9601 taatcacatt ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta
     9661 cggtacaaga tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga
     9721 aagtcattac tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat
     9781 cacgcagtgc aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg
     9841 gcagaattaa agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg
     9901 gaacaacaaa tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca
     9961 aaacgattaa agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct
    10021 attgttgctt tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa
    10081 gaaatcacgt caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa
    10141 caagaaaaaa acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga
    10201 ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt
    10261 cggcggttta tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc
    10321 attttatttg ctacgagtat tggattattc tttttatttg ccgtccgttt ctcgtatatc
    10381 gttattggag gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa
    10441 ggaagtgaag tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc
    10501 gcagaagatg cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg
    10561 gataaaaaac tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag
    10621 aatctttcta tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag
    10681 ctgtatcaag ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac
    10741 atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct
    10801 cggatgtatc caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa
    10861 gatgagaatg gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc
    10921 ggtaaagatg gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca
    10981 gtggcagaag aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga
    11041 ctacaaagtt atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa
    11101 ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct
    11161 actttcaatc ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc
    11221 ttggtacaag atagttatga accaggatca accatgaaag ttttcactac tgcagctgct
    11281 atcgaagaag gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa
    11341 gatgctacga tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct
    11401 ctttcatggt caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg
    11461 tataactatt tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa
    11521 gtaaacggtg ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa
    11581 gcggtaggtg tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt
    11641 acaatgattc aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga
    11701 acaactcaaa cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt
    11761 gaatatatga gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt
    11821 gtacctggat acaatgtatc ggcaaaaacc ggaacagccc aaatcgcttc tgatacaggg
    11881 ggatatcaaa caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa
    11941 gatccagact atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca
    12001 ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa
    12061 gattcagata cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa
    12121 gcggacgttg cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat
    12181 ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa
    12241 ctgatcctat atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca
    12301 gatatcatga agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat
    12361 tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact
    12421 ttagaagaat aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat
    12481 tcccattgtc agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt
    12541 tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa
    12601 agccggaact cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat
    12661 ttgggttgga gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt
    12721 agcgttatac ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa
    12781 catgggactg aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta
    12841 tcttgtttac cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc
    12901 tttaggaatc atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt
    12961 aaatctgaca gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc
    13021 ttacggtcta attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt
    13081 tttaggaggt ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg
    13141 agatgtcggt tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca
    13201 agaatggaca ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct
    13261 tcaagttact tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca
    13321 tcactttgaa atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag
    13381 cattgtgact tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa
    13441 aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa
    13501 gtgcggccaa attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt
    13561 ttgacgaaaa tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta
    13621 gtcatccaat cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc
    13681 cctacagtca tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag
    13741 ttgagctagc ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa
    13801 aaacaacgac aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg
    13861 cacgcttagc gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag
    13921 aagataaaat tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc
    13981 ctcatatagc ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa
    14041 aagaatatgt caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga
    14101 tccttaactg gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac
    14161 tgcctttctc gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat
    14221 actataaaaa agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg
    14281 tagaaaatgc actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta
    14341 tacgagaaac gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa
    14401 tccaaggcag aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg
    14461 ctctaagtgg atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa
    14521 atacatttga tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc
    14581 aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag
    14641 ccgattctgt agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag
    14701 ttttattatc accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa
    14761 acagattcat ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa
    14821 atgaaaattt tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt
    14881 gtcaattatg taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga
    14941 ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa
    15001 ggatttaaac gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt
    15061 atccgtcaag caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga
    15121 ggatatgttt ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc
    15181 catgaacaaa acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg
    15241 atcgctcttt catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta
    15301 ggtaatcctc gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt
    15361 gatttggatc cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa
    15421 atcaatcaag cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg
    15481 tatgcttcag gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa
    15541 aatattagtg tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg
    15601 ctggtggggc gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca
    15661 gtgttggttc ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt
    15721 gttcacgcag gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt
    15781 cagacagtca acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct
    15841 aaagaacaag gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc
    15901 aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc
    15961 cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag
    16021 aacaagagat gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt
    16081 cagattctga agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga
    16141 aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt
    16201 cttttttgaa ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt
    16261 tagctttgat catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc
    16321 cttatagcaa attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa
    16381 tcatctctga tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat
    16441 ctacttatat cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt
    16501 tcaaagggat gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg
    16561 caaaaaacaa tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag
    16621 caaatcctac aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag
    16681 aattaacaaa gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca
    16741 agtacacgcc aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc
    16801 aagttattgt aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca
    16861 aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg
    16921 gtagcactca agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa
    16981 atgcggatca aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg
    17041 gatcgtctag cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag
    17101 aagataatcc aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa
    17161 agtaagaatg tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata
    17221 tctattaata agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa
    17281 gtaggaggga atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac
    17341 gacatctgtt aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt
    17401 aggaaacgca aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt
    17461 ccaagcgata caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg
    17521 cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc
    17581 agttaacgga gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc
    17641 ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac
    17701 tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat
    17761 gtacggtctg ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga
    17821 aaatgccgga ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat
    17881 tctttctgac ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac
    17941 aacaacggct gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg
    18001 cgaatttgtc actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc
    18061 gttgaaaata aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc
    18121 agtagatgtg attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat
    18181 tatttcagct cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga
    18241 agcattagag ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt
    18301 tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat
    18361 ggggctgaga aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt
    18421 gagtgaagtg tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca
    18481 aatggtagtt gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac
    18541 aatctacgat gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg
    18601 tttctttaca aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa
    18661 ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg
    18721 gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca
    18781 acacagacgt gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca
    18841 aatatactcg tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag
    18901 aaagtgaaca atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag
    18961 gaatgggtgg aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat
    19021 tgggcgcatt gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg
    19081 gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta
    19141 tctcaaataa ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc
    19201 gtgaagcaga taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc
    19261 caggttacgt taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag
    19321 cattgatggg gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag
    19381 caatctcctc tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca
    19441 tcacaggcgg tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa
    19501 atgcagcaac tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg
    19561 atgaaatccg tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt
    19621 ctagccgacc agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata
    19681 tggatcaagc taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca
    19741 gacgtgaaca aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa
    19801 aagaatttga tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata
    19861 cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac
    19921 ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa
    19981 gaagttacat tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt
    20041 agagctggcg ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa
    20101 gatttatttg attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag
    20161 gtaaaattga ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca
    20221 gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct
    20281 ggtgaggaat caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta
    20341 gctgcctgca caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct
    20401 gatgagcaaa tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca
    20461 aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa
    20521 attgcgattg aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa
    20581 taaaggagga acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt
    20641 agctgatgag gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca
    20701 gccaactaaa aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac
    20761 tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc
    20821 tgaaaagaaa aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag
    20881 aacagcttca agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat
    20941 ggaaccaaga gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc
    21001 aaccttagtg aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct
    21061 aacaggaaca gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct
    21121 ttgtacaccg actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa
    21181 tttgtttgat ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta
    21241 cttcaacggg cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg
    21301 tttccaggcg ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat
    21361 atcagtttgt tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg
    21421 gcaatcctga tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact
    21481 gtccatttag tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg
    21541 aaaattttgg atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat
    21601 tcggtaggcg attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt
    21661 cttgacccaa gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt
    21721 ttcatgtttt atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat
    21781 tattacgaac ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc
    21841 aaatttgcca gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa
    21901 cgctcttact ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat
    21961 gaggtggatc attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg
    22021 gaaccaatca aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga
    22081 acagttagtt ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag
    22141 cggtccaagc agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga
    22201 cctgattttg tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag
    22261 atccaagaac ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta
    22321 cgtaaataaa ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata
    22381 agacattccc gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt
    22441 tagttgttcg tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca
    22501 aacattctga agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa
    22561 tcatcgtagc tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag
    22621 tgatcgttac ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg
    22681 cacatcaatt aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag
    22741 aaaaagcaag acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc
    22801 atcaacgtat cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg
    22861 atgaaatttt acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag
    22921 ttttagcaaa acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca
    22981 tagaagcacc tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg
    23041 aaaatgaaaa cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag
    23101 tggaagttcg gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga
    23161 aattagagta agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata
    23221 gatgcaacta aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt
    23281 tttgtgtccg gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc
    23341 aattaggaaa aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc
    23401 aaaaagaatg ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac
    23461 ctagctttgt tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt
    23521 cattgaataa aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg
    23581 caccatatgt tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata
    23641 aaggcgtgaa acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg
    23701 cactttccca agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt
    23761 gggaacatcc ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg
    23821 gtaaaatggc tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt
    23881 caagtgagtc ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta
    23941 tttttgtagc tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg
    24001 tcatttggac gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa
    24061 aatttacgta tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc
    24121 tagaaacagt ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg
    24181 gacaagaaat ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga
    24241 ttttaggcga tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac
    24301 atggggaaga tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag
    24361 actcaaaagg attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag
    24421 caaatcctat gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct
    24481 ttactcatag ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc
    24541 cacaatggtt tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag
    24601 tagagtgggt tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg
    24661 attgggtaat ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa
    24721 atggagaagc aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac
    24781 atggatcaat tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc
    24841 acccaggttc tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg
    24901 attctggttc ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag
    24961 atatgtattt agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta
    25021 gtgtggctat caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag
    25081 atggtgaagg ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga
    25141 tcaaacaatt tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag
    25201 atgttcgtgt ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa
    25261 atacgatgcg tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt
    25321 cgtacaatga tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca
    25381 aagaaatccg agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta
    25441 tgaacttctt gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt
    25501 atattgaagc agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag
    25561 ctgtagcttt gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt
    25621 cattcctgaa agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt
    25681 ttgcaaacca agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg
    25741 tcttaaaagc attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa
    25801 aactaactgt ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata
    25861 ttgcacaatt attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag
    25921 tgccagataa cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg
    25981 aaacttgcga tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc
    26041 atctatgcgg tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag
    26101 gattcgaaga aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt
    26161 tgttatcaat cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat
    26221 tcgaatccgt ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg
    26281 tagttgggaa aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc
    26341 caagattgag cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct
    26401 aatagtaatt tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat
    26461 tccaatttga ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa
    26521 gaaaatcctt cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg
    26581 tcacatggtt catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg
    26641 ttgatccgag tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct
    26701 gaccaacgga agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga
    26761 gcatccactt tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga
    26821 gcatattgcc cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct
    26881 ttcaatgctt tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc
    26941 attgcaagta atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca
    27001 ctatgatcgt agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg
    27061 atattgtcga tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata
    27121 gctgagatgt tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt
    27181 tctgggaata cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg
    27241 aacggttttt ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc
    27301 aaatgagaaa cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta
    27361 ccgtttaatt gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc
    27421 gttgaatcat ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct
    27481 tcatctgaag gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg
    27541 ctccagggac ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg
    27601 taaaggcgga acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat
    27661 atcgtaaaca atacattttt ttcattagac atgtggatac actcctttat attttatcga
    27721 caaaaatcgt gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag
    27781 tgatcggtcc ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt
    27841 caatagtgac taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt
    27901 ttgtaccgac aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt
    27961 ttttaggaat cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat
    28021 gatctgcaag atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat
    28081 tccagctata gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca
    28141 gactgctagc tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt
    28201 ctacaagctc ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat
    28261 ctagactaga tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa
    28321 tgattttcaa ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa
    28381 accatcatta gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac
    28441 aaaggatttt tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt
    28501 tcgtttataa aggatatata tatacattgg gagggatcta tttttcagat aacaacttat
    28561 atgaagtgtc tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa
    28621 aaccatactg atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa
    28681 gagactgacc gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct
    28741 ggctatcact aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt
    28801 gttttttctt tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat
    28861 agatcatttt ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt
    28921 ctttcttctt tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg
    28981 gtttttttac tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat
    29041 aatttgaagt atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata
    29101 gcacattgta tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga
    29161 ggaaaagagg tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa
    29221 cggaaaaccg cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat
    29281 agtcaattgc ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca
    29341 ggattaactg gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt
    29401 gatatttatt tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca
    29461 cttgaaaata aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat
    29521 ataggagaat taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct
    29581 cagttcgaac atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat
    29641 atggttcgac atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg
    29701 gcagatgagg taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat
    29761 cctatacaat agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg
    29821 attactcatc aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta
    29881 tattctagac atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa
    29941 ttcgcaacag atttgaagac ttttttcaat attactcata tggacaccat ttatattgct
    30001 tctccgaata gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg
    30061 atagttgaaa agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca
    30121 aatgaacaca atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa
    30181 aagattgccg attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg
    30241 aaatattctt cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact
    30301 catttctcag gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct
    30361 tggtttggaa tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat
    30421 ggtcttggat gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa
    30481 atcggtgatt cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat
    30541 ggagtcaatg caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat
    30601 ttggcggtaa cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc
    30661 atggctcatc cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct
    30721 agaaatgtga accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca
    30781 gataatgagg aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga
    30841 aaaaatatga ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc
    30901 cactaagtaa taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg
    30961 cttatctttg gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt
    31021 ttgcttcttc tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag
    31081 acaaagagtt gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg
    31141 gtttgaagga aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga
    31201 aagcaaaaaa aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc
    31261 tatttgacgc tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt
    31321 atcagctagc ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca
    31381 ctgggaaaac gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga
    31441 gacattactt tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc
    31501 atatagaaga ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg
    31561 aagaaaaatt gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc
    31621 tactccgaaa agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca
    31681 cagatgtggc ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag
    31741 ttccgttaag tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg
    31801 aaggaatagt aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac
    31861 gtccatcaaa tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc
    31921 agccaaccaa agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg
    31981 gaaaggagca gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat
    32041 caaaaaaaga gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta
    32101 tgaaaacggg aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga
    32161 gatcttgcag ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg
    32221 cttaactatc gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca
    32281 tattgtaaaa accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc
    32341 tgatgaagtg catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa
    32401 tgattcttta aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg
    32461 gttgcatacg attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat
    32521 gtatcatttt gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa
    32581 ttgtgcggat tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa
    32641 aggggcttac acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg
    32701 tatattgttt ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac
    32761 tttcattgat aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc
    32821 tgttcagatc attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg
    32881 acggattcct atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta
    32941 tgaaatcatc tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat
    33001 cgaaaaagaa gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt
    33061 gaaacgagac ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga
    33121 gaaaaagcta atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa
    33181 aatcaaggaa ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac
    33241 atttgaacca ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt
    33301 ctataacgat attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga
    33361 tctagagacc ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt
    33421 gactaaaagt tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca
    33481 attgtatgat gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc
    33541 ttttgctttg catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca
    33601 cccagatttg aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct
    33661 ttcaacgatc attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat
    33721 tagtatgttt ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga
    33781 tgttctcata ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga
    33841 actattagga acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac
    33901 aatgtatgaa gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct
    33961 actcttgtta acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac
    34021 tggcattcgg acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg
    34081 gatggcaagt gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga
    34141 atcgatcgta cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc
    34201 cgtaatcgtg acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat
    34261 gccagtgatt aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc
    34321 attcaaaaaa cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac
    34381 agttgatgtt gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga
    34441 aaaactaaat attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga
    34501 ttcacttgct ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca
    34561 aaagacagtt cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac
    34621 tggaatcgtt atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg
    34681 gcaatttgag aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg
    34741 ggcaaacttg atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc
    34801 atttcttaca caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg
    34861 tttacacact aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct
    34921 atttcagctc ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa
    34981 aaggagtgtt aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta
    35041 cgagtttatt ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata
    35101 tttttgcggt ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat
    35161 tattattagg tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta
    35221 aaggtattcc attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag
    35281 ttcttgcaca agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa
    35341 cgtggaagat caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc
    35401 aaagagcgat gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga
    35461 taggaaaatc gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat
    35521 gttgacacaa gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca
    35581 agatcactta atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta
    35641 tcgtcgcgta tacagcgaat aaatacaact tcttttttga acggattttt tattttgtta
    35701 taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat
    35761 caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt
    35821 tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg
    35881 ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg
    35941 acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg
    36001 gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt
    36061 ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta
    36121 ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc
    36181 cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca
    36241 cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt
    36301 ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca
    36361 acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca
    36421 ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg
    36481 tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag
    36541 gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt
    36601 tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc tggggacaag
    36661 cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag
    36721 ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg
    36781 cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt
    36841 tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca
    36901 atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc
    36961 cattcttgat tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc
    37021 tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct
    37081 tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt
    37141 tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac
    37201 aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt
    37261 cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa
    37321 ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc
    37381 aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa
    37441 ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa
    37501 atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt
    37561 ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt
    37621 atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa
    37681 agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag
    37741 catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg
    37801 gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa
    37861 ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta
    37921 catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga
    37981 cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg
    38041 atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag
    38101 atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat
    38161 gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag
    38221 cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc
    38281 gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag
    38341 gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga
    38401 ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga
    38461 gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg
    38521 gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc
    38581 ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga
    38641 taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa
    38701 aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat
    38761 gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa
    38821 gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata
    38881 tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca
    38941 tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt
    39001 tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc
    39061 ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga
    39121 aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta
    39181 tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc
    39241 aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct
    39301 attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa
    39361 agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta
    39421 tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg
    39481 gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt
    39541 tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc
    39601 cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc
    39661 attggaaagg acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac
    39721 tagtggaaac gttttgccta acttataagc tgattttttt agcagataag cttgcaagta
    39781 aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag
    39841 caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc
    39901 cctgtatcct ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta
    39961 gttatcattt ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc
    40021 aagctctcga acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa
    40081 acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg
    40141 ccttagtcca tgcaaagtaa attgcttttc aatccctaat tcttttaatt ttttgcgcaa
    40201 atatttgctg acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt
    40261 atgagggttt ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt
    40321 taagagatca atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt
    40381 ttttccccat tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc
    40441 gaaatcgata tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc
    40501 agcaactaac acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt
    40561 cattaaagat ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc
    40621 ttttcctttg attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc
    40681 atctttcaac gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc
    40741 tttagcatac ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac
    40801 cgctggcata tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg
    40861 agatactgtt ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag
    40921 atttttgcga tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc
    40981 ttttgcttct ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc
    41041 ttttttgtat gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat
    41101 tgaatcagct ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt
    41161 tattattttt tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta
    41221 atggtataat tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg
    41281 atttttaaaa gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc
    41341 tggtttttta ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag
    41401 taagagttga gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt
    41461 tgccgacatc acctaatact atggagctaa tcgttttttc taacagacca aaatcagata
    41521 atctatttaa gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag
    41581 tgaaattttc gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta
    41641 attgattcag tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa
    41701 gtaaacaatt cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt
    41761 ctacctcgtc atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga
    41821 aaacatcttc tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga
    41881 atttctcgat gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa
    41941 cgccaccaat ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt
    42001 ttttggttag tttttcattg ctattatcca tagttacctc ctattttgtc atttccttta
    42061 caagcattgt gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta
    42121 actcttagtg gggcgtggtt catttatttt ttacttactt tactattaat catatcccat
    42181 tgagcatagg atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact
    42241 ttatcgccct ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa
    42301 actttaggtg ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata
    42361 atggctattc tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca
    42421 ctaatatatt tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta
    42481 tctaagaaag caccccagtc atgattattt gtctctaaaa attcttcaaa ttgagttagg
    42541 cttttattgc tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg
    42601 ttattgtcat ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc
    42661 ccgataataa ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt
    42721 aacatgatta aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt
    42781 aatagatcct ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta
    42841 aaatcgaatt cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt
    42901 tcatctgcac ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc
    42961 tctatatatt tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg
    43021 ttttcatctt tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta
    43081 aattgtatat aaccattact catacctggt ttttttaatt gtacggctga tatattttta
    43141 aaggggatag ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta
    43201 ataattgaaa tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa
    43261 atctcttttt ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca
    43321 tatatgagga aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt
    43381 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    43441 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    43501 tccataaatt tttcgagatt aaactgagat ttatcagtca gttcgttctc aatatagata
    43561 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    43621 gtagaagtat cgtacaaaac tgaaaattca aaa
//
LOCUS       DACCDF010000016        39958 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCDF010000016 DACCDF010000000
VERSION     DACCDF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.016"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IVK51_000843"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IVK51_000843"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP6430455.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IVK51_000844"
     CDS             1011..2258
                     /locus_tag="IVK51_000844"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP6430456.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IVK51_000845"
     CDS             2334..2678
                     /locus_tag="IVK51_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430457.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IVK51_000846"
     CDS             complement(2795..3121)
                     /locus_tag="IVK51_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430458.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4696)
                     /locus_tag="IVK51_000847"
     CDS             complement(3494..4696)
                     /locus_tag="IVK51_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6430459.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            4813..5328
                     /locus_tag="IVK51_000848"
     CDS             4813..5328
                     /locus_tag="IVK51_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6430460.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5527..5973)
                     /locus_tag="IVK51_000849"
     CDS             complement(5527..5973)
                     /locus_tag="IVK51_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6430461.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IVK51_000850"
     CDS             complement(6020..7498)
                     /gene="msr"
                     /locus_tag="IVK51_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6430462.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7852..8481)
                     /locus_tag="IVK51_000851"
     CDS             complement(7852..8481)
                     /locus_tag="IVK51_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6430463.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8465..8929)
                     /locus_tag="IVK51_000852"
     CDS             complement(8465..8929)
                     /locus_tag="IVK51_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6430464.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9416..10684)
                     /locus_tag="IVK51_000853"
     CDS             complement(9416..10684)
                     /locus_tag="IVK51_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6430465.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10915..11652)
                     /locus_tag="IVK51_000854"
     CDS             complement(10915..11652)
                     /locus_tag="IVK51_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6430466.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11719..12288)
                     /locus_tag="IVK51_000855"
     CDS             complement(11719..12288)
                     /locus_tag="IVK51_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6430467.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12416..13288
                     /locus_tag="IVK51_000856"
     CDS             12416..13288
                     /locus_tag="IVK51_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6430468.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13630..14502
                     /locus_tag="IVK51_000857"
     CDS             13630..14502
                     /locus_tag="IVK51_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6430469.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14499..16457
                     /locus_tag="IVK51_000858"
     CDS             14499..16457
                     /locus_tag="IVK51_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6430470.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16708..17559)
                     /locus_tag="IVK51_000859"
     CDS             complement(16708..17559)
                     /locus_tag="IVK51_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6430471.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17682..18149
                     /locus_tag="IVK51_000860"
     CDS             17682..18149
                     /locus_tag="IVK51_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6430472.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18412..18894)
                     /locus_tag="IVK51_000861"
     CDS             complement(18412..18894)
                     /locus_tag="IVK51_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6430473.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18994..19857)
                     /locus_tag="IVK51_000862"
     CDS             complement(18994..19857)
                     /locus_tag="IVK51_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6430474.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20455..21303
                     /locus_tag="IVK51_000863"
     CDS             20455..21303
                     /locus_tag="IVK51_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430475.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21474..21815)
                     /locus_tag="IVK51_000864"
     CDS             complement(21474..21815)
                     /locus_tag="IVK51_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6430476.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21901..22608
                     /locus_tag="IVK51_000865"
     CDS             21901..22608
                     /locus_tag="IVK51_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6430477.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22595..22918
                     /locus_tag="IVK51_000866"
     CDS             22595..22918
                     /locus_tag="IVK51_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6430478.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23007..24044)
                     /locus_tag="IVK51_000867"
     CDS             complement(23007..24044)
                     /locus_tag="IVK51_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6430479.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24491..25474)
                     /locus_tag="IVK51_000868"
     CDS             complement(24491..25474)
                     /locus_tag="IVK51_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP6430480.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25890..26660)
                     /locus_tag="IVK51_000869"
     CDS             complement(25890..26660)
                     /locus_tag="IVK51_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430481.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26653..27588)
                     /locus_tag="IVK51_000870"
     CDS             complement(26653..27588)
                     /locus_tag="IVK51_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430482.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28182..29831)
                     /locus_tag="IVK51_000871"
     CDS             complement(28182..29831)
                     /locus_tag="IVK51_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6430483.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30026..30922)
                     /locus_tag="IVK51_000872"
     CDS             complement(30026..30922)
                     /locus_tag="IVK51_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6430484.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30915..31595)
                     /locus_tag="IVK51_000873"
     CDS             complement(30915..31595)
                     /locus_tag="IVK51_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430485.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31596..32378)
                     /locus_tag="IVK51_000874"
     CDS             complement(31596..32378)
                     /locus_tag="IVK51_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6430486.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32371..33288)
                     /locus_tag="IVK51_000875"
     CDS             complement(32371..33288)
                     /locus_tag="IVK51_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430487.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33291..33575)
                     /locus_tag="IVK51_000876"
     CDS             complement(33291..33575)
                     /locus_tag="IVK51_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6430488.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            34363..37590
                     /gene="fss3"
                     /locus_tag="IVK51_000877"
     CDS             34363..37590
                     /gene="fss3"
                     /locus_tag="IVK51_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP6430489.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            37703..38017
                     /locus_tag="IVK51_000878"
     CDS             37703..38017
                     /locus_tag="IVK51_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6430490.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            38030..38404
                     /locus_tag="IVK51_000879"
     CDS             38030..38404
                     /locus_tag="IVK51_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6430491.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            38414..38749
                     /locus_tag="IVK51_000880"
     CDS             38414..38749
                     /locus_tag="IVK51_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP6430492.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            38832..>39958
                     /locus_tag="IVK51_000881"
     CDS             38832..>39958
                     /locus_tag="IVK51_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6430493.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRD"
BASE COUNT        12155 a         8142 c         7384 g        12277 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
     4441 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
     4501 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
     4561 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
     4621 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
     4681 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgta
     4741 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
     4801 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
     4861 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
     4921 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
     4981 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
     5041 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
     5101 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
     5161 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
     5221 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
     5281 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
     5341 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
     5401 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
     5461 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
     5521 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
     5581 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
     5641 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
     5701 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
     5761 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
     5821 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
     5881 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
     5941 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
     6001 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
     6061 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
     6121 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
     6181 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
     6241 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
     6301 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
     6361 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
     6421 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
     6481 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
     6541 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
     6601 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
     6661 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
     6721 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatccg
     6781 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
     6841 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
     6901 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
     6961 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
     7021 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
     7081 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
     7141 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
     7201 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
     7261 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
     7321 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
     7381 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
     7441 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
     7501 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
     7561 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
     7621 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
     7681 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
     7741 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
     7801 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
     7861 cgtagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
     7921 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
     7981 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
     8041 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
     8101 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
     8161 catcttccct gattcgtaag cttggttata gatttcttcg ccatattcgt aacattgcat
     8221 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
     8281 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttg
     8341 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
     8401 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
     8461 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
     8521 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
     8581 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgctcg attttcttct
     8641 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
     8701 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
     8761 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
     8821 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
     8881 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
     8941 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
     9001 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
     9061 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
     9121 ttgattctta tcaaactata gaagatagct cttctgcagt agaaaatttt acaccgccaa
     9181 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
     9241 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
     9301 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
     9361 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
     9421 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
     9481 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
     9541 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
     9601 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
     9661 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
     9721 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
     9781 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
     9841 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
     9901 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
     9961 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    10021 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    10081 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    10141 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    10201 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    10261 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    10321 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    10381 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    10441 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    10501 ttgtattttc tgagacagta gctgactgca aagtactctc tgtatcgttt tcaattcctg
    10561 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    10621 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    10681 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    10741 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttgata aatttgcttt
    10801 ttctacataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    10861 tcgtttgaat aactatttaa caagtaattc tctttggata ttatccgtta tggattatac
    10921 ttcctgtgtc gtcggacgga taatcatgtt actgacagac atccggtctg gcgtttctat
    10981 cgcaaatgct acagcagaag ctatatcttc ggaggtcaat ccccattctt tttgcgcttc
    11041 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    11101 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    11161 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    11221 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    11281 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    11341 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    11401 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    11461 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    11521 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    11581 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    11641 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    11701 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    11761 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    11821 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    11881 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    11941 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    12001 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    12061 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    12121 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    12181 tgctaaaggc tcttcgtttt cagcttggat ctgatggatc acttcaaaga gcggatccgt
    12241 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    12301 ttttatcctt actttataac ggacggctta tcatgtctaa tacttataaa acatacttaa
    12361 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    12421 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    12481 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    12541 acttggtgtg acacttttca ctcggggctc ccgagagata cagttgacgg attctggtcg
    12601 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    12661 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    12721 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    12781 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    12841 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    12901 agatcgttgg ggacttttga tacatcgaaa tcatccttta gcaaagcaaa ccgtcatcca
    12961 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    13021 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    13081 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    13141 gatcatcaat acttatggga cggaaaaaat attcatccca ctcgatccgc cattacaagc
    13201 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    13261 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    13321 aacaacgaat aagataagca aaagatccaa cgattaaata acataagttt cttaatttat
    13381 ttcttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    13441 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    13501 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    13561 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    13621 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    13681 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    13741 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    13801 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    13861 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    13921 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    13981 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    14041 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    14101 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    14161 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    14221 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    14281 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    14341 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    14401 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    14461 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    14521 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    14581 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    14641 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    14701 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    14761 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    14821 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    14881 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    14941 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    15001 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    15061 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    15121 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    15181 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    15241 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    15301 acaaagcaaa aacagcttcc ttgatgctag cagcagcttt tgcttcattc tttactggtg
    15361 tgacagaacc acttgaattt tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    15421 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    15481 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    15541 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    15601 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    15661 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    15721 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    15781 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    15841 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    15901 ttgcagatga gatggcaaaa ctacatggtg gtgctgcagc acgacctgca caaacaaaca
    15961 ccgtggttaa aacagaaaca tttgccggtg aaacgatcga acaagatatt tacgctatcg
    16021 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    16081 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    16141 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    16201 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    16261 atgtagaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    16321 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    16381 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    16441 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    16501 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    16561 actccgcggt aataagtcca gctaaacgaa acatatgaag agctattttt tatttgactc
    16621 ttcttattac ttgatgcaga acagcttttt cacaaaattt ttcttcatac tcaaaactaa
    16681 tcggcttttt cacaacctct ttatacatta ttcctgatcc aatatttctt ttaatgctgc
    16741 ttcaagagaa gttaatggtt ttcccaatac tttttctaaa tcatcagagg aaatagctaa
    16801 ctgatccttt ttaatatcat gttggatcgc taaaaacatg tcagctgctt cttgagggaa
    16861 tccggcttct tttagatttt cgacaaacgc tgcatcactt gcttcaataa tttctaactc
    16921 tttacctgtc gcacgctcta atgccttggc taattcttca tagtcagtta gattgccgga
    16981 taattctaag atttctggag agtcactttc tactagtact tttgctgcag cttctgcata
    17041 ttcacgtttc aatgcccaac ctgtttttcc atttcctgca gcataaacga attgaccagt
    17101 ttttaaagcc tgcccaatga taggtaattc attctctaca taccaattat tccgtaaaaa
    17161 tgtatgggaa atacctgaat ccttaatcat tttttcagtg aattggtgat ctggcgctaa
    17221 tatactgatc gagtgatcgg catctgcaaa acttgtataa gcgatataag ataccccagc
    17281 tgcttgtgct gccttcacta cattcttatg ttcggcttga cgatttcctg gaacgccaga
    17341 gacaaaaagc aatcgatcta ttcctttcaa cgcttctttc attgagtcta aatcatcata
    17401 atcaccaata cgtatattaa atccagcttc tttcagtgct tctccttttt cttcactacg
    17461 tactagtaca tagatttcat tttgtggaac catctttttt aagaattcca gcgcataacc
    17521 accaaaacct ccagttgctc ctgttactaa ataatgcata tttgccactc cctattcttt
    17581 ttatttgtta agatttacag ctgtacattt ataaattaca cctttatcat aaagagtgtg
    17641 gtaagataag tcaagcaaaa aaaatgaaag gatgacaact gatgaagtat tctctgcaat
    17701 ttagtgatgc aatccatgta cttgcctata ttgaaatgtt tcaagatacc cgatcacttt
    17761 ctagtgagat gattgccagt agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga
    17821 gtcagctcaa aaaatccggt ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg
    17881 ccaaaagtcc agatcagatt tctttactag atgtctatcg cagcatcgaa ggtaatacac
    17941 agcttatcca agtcgatcca aaaacaaatc caaattgcgt cgttggtgcg aatatccaag
    18001 aagctttaga agaaagctac gctcggctgc agaaaaaggc agaagaagaa atggctaaga
    18061 tcacacttga tagtctgata cataaaatag ccgttttgga aaaagaaaaa agaccagaaa
    18121 acacaggcct cgtagagaga tttttataat tctctctact gtgcctgcat ttttctgatc
    18181 ggaatcgtct acataaattc ttttattctc cgggggacaa aagccttgtc atgccccttt
    18241 ttcctctcta atgctcaggt caaagcgctg aagtcatgac gtcttgataa acggtgttca
    18301 agtagctgac cctcggcatt agttctgaaa aatgcaaaat atggaaagct atttcccatt
    18361 tttcctctct aatgctcagg tcaaagcgct actttcacga cctctatatt atcatggttt
    18421 attgactacc ggtatccaga tttctcccat atatttcccg ttggtaactc ccatttcgac
    18481 tacagcattt ggacccccaa cataggcgta atcttctact tctcttaaaa catgcccaaa
    18541 ggccgtattc gtcaaatcat ttcttaattc ttcaggcgat tctgctaaag cgcttacaac
    18601 aagatacgtt ccttttggga atgggatgat tcgcgtagtg tttggaacat cctgactcgt
    18661 ttgaacaccg acatagtaca tcattttccc gttatagggt tcgttgactg taaagaaata
    18721 atgattttca gcagcttgtt tcaatgcatc tattgttcca ttaccgataa ctttttgctc
    18781 aaatgcttgt ttctctgcag ccaaaccgac aaaatccgta ttgctactct tcaattccac
    18841 accaataccc gtcaccgtaa aatctggttt ttcttcgatc ctatactttg ccatttctcc
    18901 caccctttct attatattaa gttcatctta cataaaatta aagggaaatg acacgattac
    18961 gctctttttt ctagggatat ttttattttt taactatttt tttgctttca ctgcaatcca
    19021 aatctcactg taaacattac tgaaatcctc tccccatttt gtaaaagaga tctcgggagc
    19081 ttcgactaac ccataatctg aagaaggaag ccattctgca tagattttcc cccaagtatc
    19141 ttgtaaagtt tggggaaatg gccctttatt aggaaaaatg gcccaagtgt gttcttctat
    19201 agcaagcatt tctaaatcag cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg
    19261 catcaaactg cccttttctt ccaagcggtc tccatcaaat ccataagaaa cgttgattac
    19321 ttgatttgga tataaatctc ctaacttatg catctcttcc ctttgagtct ccgtgatcgt
    19381 ctgcgctaac ttttgtattt cttgattgac tccttcaaat ttgacaggaa cttttttcgc
    19441 cacacctgcc acataaaact gttctttctt ttcgatacga aattccatcg attttcctcc
    19501 tctgacatca ataaaaaatg tcaagcgtgg aaaagatttt tgtatgccgt atctactgat
    19561 ttctgaaggc agatatcctg accaatcacg aaaagctctt gaaaaacctt cgactgtttc
    19621 atatccatat ctaaacgccg tttctgtcac tcttgatcca tctagaagat cgcgattagc
    19681 caaagccaat ctacggtttt tgatatagtc cattagcgac atccctgcaa taaacgtaaa
    19741 gatgcgtttt aattgatatt cagagattcc tgtattttcg gctattattt tataggaaag
    19801 tccttctgtc aaatgttctt cgatgtaatc gatcaattgg ttcagatctt taagcatatt
    19861 tatcttcctc cttgattgcc tttttatcat attatcaaag aaagtcaatt acttgatgaa
    19921 tcaggaacca atttagtcag atgtagaaaa tgttgtatgg atctaaagaa aattctcaag
    19981 cagcgtttcg taacggataa ttggtattat ctgcctatct atgttttacc tttagaaaaa
    20041 taaattttgg ggaagacaga ttctaagtaa acaaagcggt aaaaatatct tttattccct
    20101 tagttaatcc gcttactacc tactgtacaa agcaaattgt tgttcaaact gctgatcaga
    20161 acgctgcttg gatttttctt tgcgatacat catacctctt ttatcaattg gaacaattat
    20221 ttatttctaa caaaatcact tcatttttgt cagataaata aaaaacaaaa aaagttccat
    20281 ctttaaaaca cgctattctt ttactttttc tcacattctc taagaaaaga aaaggagaat
    20341 gaatttttga caattcgagc aaaagtgatt atcaaatatg taaaatcagc tacttttttc
    20401 ttaaacgttt ctttatacta aatataaata agtgtttcat gaaaggagaa ctagatgaat
    20461 aattggcttg agtattttcc tgaaaacgtt ttggaacgcg gttacagcta tcatctgcac
    20521 ggttttgtcc gtcatttgaa ctatacaagc aagtacttgt ccgcaacagt ttctggcacg
    20581 gaagattata aggtagtgat cacttgggat gaaaagacca acatgacttg tgattgtcta
    20641 tatgccatag aaggaaaaaa atgcaaacac atggctgctg tcttattcgc ttacgaagaa
    20701 cgtccaataa aaaaatccaa ctactcactt tccgaactct cgtcccttgt ttcctccgct
    20761 agttcctctc ttgtacggga gcttttaaca gagattctta tagaacatcc gcactttatc
    20821 gaacgcttca aagttaagat gccatttcat gcaataaatt attcagataa attaacaact
    20881 atcatccata aatacgatca tattatcaag aaaaacaaaa acaggaaaac agcgaagttc
    20941 atcatggaga tgcgaaagtt catccaagaa gcagttgaat cgcttattca gcaaaatgcc
    21001 tatcttcccg cattcgaatt gatcaatgaa gtgattgcta ctctcgaaac attctattgg
    21061 gagcctgagg atgaacgaac ccttcttctg atagaggact gttattattt atggaaagaa
    21121 ttattagcag aagcacctca tgcagaaaaa aggcaaatgt tctcttggtt cgtctgccaa
    21181 gtcgatcata ctgacgcctc ttattcaaaa cgctactcta taaaaatcct gaaagaagac
    21241 tttcgggaaa aagaattttc taaccaaaag aaaaaaatag ataaaaaact aaagaaacgg
    21301 taaagaaaga cgatttcaat gaaaaatagc aagtaaacga taacaaccat cctaaatcca
    21361 caagaaatac agcagacctt gctttaaaaa cacgaatcat gcaaaggatg aagtaaagaa
    21421 gacttaacgc gtacccaata ttagtcattt gactattagg tacgcgtttt gttttataag
    21481 tcatgacaaa tttccttcgt tgctggatag attttccggc attgttgata gattttatga
    21541 tacttttcta cattttgtaa gagaggttcc gctgcatctg tatattggat aaaattctga
    21601 caacaacttt caggtgtgtc gaaccaccct aaccctagtg cagcaatcat tgttgcgcct
    21661 aatgcaggtc cttcctccac acttaaacaa tagaccgttg catcgaaaat atcagcttgg
    21721 atttgtagcc atgcaagatt tttcgcgcct cctcccaccg aaacaatacg tgtaaatctg
    21781 cgacccgcta gttctttacg tttcaatccc accattgaca aatgctcatt aaaaatttag
    21841 aatattcaca agtttaacga caattatgaa aatttatgtc gtgctaaagg agttttgtta
    21901 ttggatgaaa agttagatat aaaaactgcg atcaaagaca cgttacctac cgttttcggt
    21961 tatatcggta ttggacttgc atttggtatc gttgggaaag ctgccggatt tcatccacta
    22021 gtcgtcacgt tgatgtccct gctggtctat gctggttctg cccaatttat cacagtcagc
    22081 atgcttgcta gtcacagccc attgctttcc atcgttttct cgacctttct agtcaattcc
    22141 cgaatgattc tgatgagtat gacgattgct ccttatttca agaaaaatcg gctgcttcaa
    22201 aatctgctga ttggcacatt gctgacagat gaaagtttcg cactgggaat gaataaattg
    22261 aactatactg gacaaaagtt aaactttcgt tggatgaata cagctaattg gatttcttat
    22321 ttgacgtggg tgggttcttc tcttgttggc gcacttttag ggaattttat tactgatccg
    22381 aaaaagtttg gtttggattt cgccatcgtc gctatgttta tcgggttact ttatcttcaa
    22441 gtcatttctg atagaaacac ctctaaacga ttacagttga tcctgattgg tctcactttg
    22501 attctggtat atgtcggact gatttttatc ccaagcaact tagtgattgt agttgtaaca
    22561 ctgattgggt gcggattggg ggtgtggatc aaacatgcct tcttttgagt ttacattgct
    22621 gactatttta ggttgtactt tagcaacttg gctttctagg gtcctaccat ttgtcttact
    22681 gaaaaaattc gatttaccgc aaccgttgct tgagtattta agttttgtcc cgatcgtcat
    22741 tatgtctgct ttatggttca gcagtctatt tacccaaaat atcggacatc tgccgcagat
    22801 caatctcgaa aatgctttgg cttcagttcc tacactttta gctgctattc tttcaaaaag
    22861 cttattggtg atcgtcttgg caggaattct ttccctttcg ttgattcgct tattataaat
    22921 aaaaaaacaa gagcttatgg caaattactg tcatgcgctc ttgtttttat caaagtcaaa
    22981 taagaaaaaa atcggcgtat tcttgcttat ccgttgaacg gaccactaac aacgactttt
    23041 cctaacatat gtcctgtttc tacgtctttg gttgcttgtt tcaagttctt cgcattcatc
    23101 ccatctgaat agaccttgct caatgtactg cggataatcc cttcatttgc taaagcagca
    23161 atgtgcgcta aagcttctcc ttgcgtttcg atttcatagt tatagtctgt tttagcgaac
    23221 atatactccc agtcgaagct gatagattgg tttttccaaa cacctatatc taacggctct
    23281 ttgatcccta cgatcgttcc cacatgtcca aatggtcgga caaggtgctt gatttgatca
    23341 aagtagcggg taatatcgaa taatacagcg acatactcta cttgatcgat tcctaattca
    23401 cgaaaacttt tacttaagtc gtgatgataa tcgattggat agtctacacc tgtttttttc
    23461 agccattcaa aattcttcgg acttgcagtc gcgtagactt ctaggccagc ccaatgcgct
    23521 agttgattca aaatcgaacc gacgcccccg gatccgttga tcactaagat ttttttcccg
    23581 cgattggcat tttcttctgg agttagacca aatttttcaa ataacaattc ataagcggtc
    23641 aatgaggtca acggcaaggc tgcagcttct tcatctgaaa gattttttgg tgcaagagcg
    23701 acaatacgtt cgtcaactag ttggtattct tgattgcttc ctgctcttgt tgttgtacct
    23761 gcgtaaaaaa cgcgatcgcc gacagaaaat ttttgcactt cgtcacctac agcaacaact
    23821 ttcccgactc catcaaaacc taagatacgt aaagcatttc ttatcccgtt atcttggcga
    23881 agtttcgtat cgacaggatt aacagatact gcgttgactt ttaccagaag atcccgtgaa
    23941 ctgggtacag gtatctgttc tttttcatca ataaagcttt ttggatcatc gatcggcaac
    24001 ccttcataaa aaccgactac cttcattaat tccttttctg acatacgaat tcctccttag
    24061 cttcatcaag ttggagcatc catacagtgt tcatttcaat atatatagta tgctatttgt
    24121 tccacctttt ttattcatca taattattct cttacttaaa gtaaactatt tgcacttttt
    24181 tggttaaaaa agaggttgcg acaagacttt tgtcacaacc tcttataaac ggtgttctcc
    24241 taccaactct tcggtcacaa gtcaaaatat tcatcaaaca tgagaagctg ttttttgaaa
    24301 ttgctccttg tgactcagag ttaaacggca ggctcacaac ctctgatata aacggtgttc
    24361 aatcaactga ccctcggtat tagctcaaaa aacgacaaaa tatgaagagc tatttttgtt
    24421 tggctgttct tattacttga tacaggacag ctttttcacg acctctaatt cacggtgttc
    24481 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtctctaaga
    24541 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
    24601 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
    24661 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
    24721 tttctgtatg ttccctcggg tctttttcaa atgcataagg attcttgaaa atccctcggc
    24781 caatcatgat gccatcaacg ccatatttct cggccaattc ttctcctgtc tggcgatcgg
    24841 ggatatctcc attgatcgtg attagtgttt gtggagcgat ttggtcacgt aaagcaatga
    24901 ttttaggaat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
    24961 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
    25021 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagctg
    25081 cttcaatcaa ttgtgccgct acttctggac gaaggatcaa gccactgcct tttccacgtt
    25141 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
    25201 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
    25261 tcggctgttc atcttctgta aatgttaatc ttcctcgtac actgtctttt ccatctggat
    25321 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
    25381 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
    25441 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
    25501 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
    25561 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaaagactg tgacaaaaat
    25621 cttatcacag tccattttcc ggaataaatg gtgttcaaaa gcttgcattt gcggtaataa
    25681 gtccaaccaa acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg
    25741 cagaacagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    25801 tcgctcaaaa aacaacaaaa tatgaggaac tattttcccg tttgtttctc taatactcag
    25861 gtcaaaacgc tgatttcaca acctcttttc taacttctca atcctcttcc tttattgatc
    25921 aaataccaag taacggcgta caaaacaacg atgaataaca caagtacgat catcgatgtt
    25981 gctactggca catcgatcgt tccaaggaat ccgtaacgga aaccggagat catataaacg
    26041 atcggattga cttttgaaac agcttgccaa aagggcggta acatagagat ggcatagaaa
    26101 acgccaccta gataagttaa cggctgcaag acaaatgtag gcacgatcga tacatcatca
    26161 taagactgag caaaaatccc gttgagcagt cctgctaatg aaaacaagat tgccgtcatc
    26221 aataatgtaa tgatgaccat gaaccatgaa tacacatgaa gtggtacaaa gaacaaggaa
    26281 atgatcgtta cgagagagcc gacgagaata ctgcgaccca caccaccaat cacaaagccc
    26341 caaataatca catgcgtagg aacaggtgct accaagattt cttcgatatt tttctgaaat
    26401 ttttgagaga agaaggacga agaaacattg gcatacgaac tagtgatagc agacatcata
    26461 atcaaaccag gaacgatgaa ttccatatat gaaaaaccac ccatgtcacc gatccgccca
    26521 ccgatcattt ttccgaaaat aacaaaatac aaggatgtcg tgatcactgg cggaaccagt
    26581 gtttggaccc atatacgcaa atagcggttc gtttccttga tagctaaact ttttaatgca
    26641 gtaaaataaa gattaaacat ttttctcctc cgtatgatta ttttcttctg tgattttcaa
    26701 gaacagttct tctaaacgat tcgacttatt cctcatagaa agaactttga ttccttgtct
    26761 tgtaagctga tcgaagattc cgttcacccc ttgatttcgt tcaacttcga cagcgattgt
    26821 ctgctcatct tcaaatgtac aagcatatcc ttcaatcaca ggtttggtat catatggcgc
    26881 tagatcaaag ataaatgttt caaattgcag ttttgacaga agctctttca tacttgtatt
    26941 ctcgatcagt tcacccgatt ggatgattcc gatgttgcga caaagcattt cagcttcttc
    27001 tagataatgc gtcgtcaaaa tgatcgttgt accattttcg ttcaattctc gtagaaactc
    27061 ccacatctcg cgtctcaact cgatgtcaac tcctgctgtt ggttcatcca agatcagcaa
    27121 acgtggttca tgcatcaatg ccctagcaat catcagtctt cgcttcattc ccccggaaag
    27181 catacgtgca cggacatttc tcttttccca aagattggac tgcttcaaat atttttcgct
    27241 gcgtttcatc gcttctttgc gagagacacc gtagtagcct gcttgattca cgacgatttg
    27301 ctggaccgtt tcaaaagggt taaaattgaa ctcttgtgga accaatccaa tctgttgctt
    27361 tgctcgaact agatccttgt ccagatcata accgaagaca ttcacttttc cagatgtttt
    27421 attgactaaa gaagtaataa tcccgatcgt tgtagacttc ccagctccat ttggccctaa
    27481 tagcgcatag aaatctcctt ctttcaccga aagatcgatt ccccgcaatg cttcgacccc
    27541 acttccatag atctttttca aattatttat ttcaagcgca tttgccattt atttcctatc
    27601 tccttataga catgattctt aattgtatca tggaatacag cagatgaaaa aacaacttgg
    27661 cttccacaca aaaacacacc tgctaacaaa gaggagatgt gttttctagt tcatgatcat
    27721 cttcgataga ataactatct aaacaactct ggaactttta aaatcataaa agtaccgaca
    27781 gcaactacta cgatgacata tagaagatcc ttataactga ggttcttgtc ttttttattc
    27841 atgttaagct ttcctccgtc cttttatcga aagctttttt cttttatttt aaccgaaata
    27901 aataaaatgg aaatagttat tttcaatttc tgttttcgct gtttgtttta cggagaactt
    27961 tgccatggaa cgtgcgtctc gtcgctgatg ctaagttttt tcgataaaag ctgctagaag
    28021 gaagacagcg tggtttattt tgtatcgtct tttgctaaat cttccaagat catctggaca
    28081 gcagactcgt tcccattgaa ttctgccact tttttggcat caatgtactt cttttgataa
    28141 tcgtcttttt ggacccacgc atcatgcttc gtttgataaa gttaatcaaa cggataccac
    28201 tgacgctgtt catcgaacaa caaactttct ttttgggcct gttcaactat ggatttagga
    28261 taattcaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat
    28321 ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca
    28381 caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat
    28441 ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt
    28501 gttcctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc
    28561 agcaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca
    28621 tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc
    28681 gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat
    28741 tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac tggattaact
    28801 accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac
    28861 tctggtcggc aagtgatcgg catataggtg cgataattca aaacggctgc tgcaacttcc
    28921 aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg
    28981 agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc
    29041 aattctgctt cactcccgct cttcattcga aatgaaaaac ctaatttagg gatgtctctt
    29101 aatggtttag tttgctgttt cagctctttt tgaagtggtg tcgtcatttt tgctaactga
    29161 ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt
    29221 tgtttcttca ctagataata gaccgtattg aatactaagc caccgattgc tagatagatt
    29281 cctagtactt gccctaaaag aagcaaacct acacccacca gcggaagcaa gccaagaaaa
    29341 ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca
    29401 aaattatgat ctttttttcc taaacgagcg aattgatatt ggattctttc tctcagttga
    29461 ggattttcct gataaaaagc aattagtttt tctaactgat aatcttctcc aaaatcaaag
    29521 ctacgtaaac gctgatacaa cgcttctgaa cctacactag aataggtact gttgatccct
    29581 tcaaatactt cgaacatatc caagtcatac caagtaagat cgtcgatttc actatcccat
    29641 gaacggaatt ttttctccgt gagccacgca tccttcaaac tttcttcttt atcaaatctc
    29701 ggttgataag gaatttttcc ccaattacta cgaactgttt cctttacttt caaacgtgaa
    29761 aaaatgtcta aaacaactaa taaaataaca actgcaataa taattaaaac gataaattct
    29821 ctttggccca tatccctctc cgttccttga tttgttagtt ttagtatagc actgttgcgc
    29881 tatttgaaaa gggggtacaa aaataactgt cttcgttacc taattcgtat caatcacacc
    29941 aaaataaaaa caccttccaa aaaagaagat gtctttaaaa cgagatacaa ttttttgtca
    30001 ttcacttcag gtaaattaga gccaattaaa ccctccgcaa cttgacggag atggagaaga
    30061 tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat ttttttgaca
    30121 ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg gtataaaaac
    30181 gctggaagac tttttcaaca gaggtttgcg ctcctggttt gataggattg gccactgtta
    30241 atcgaatgaa tgaatcctct tttgaaaatg tcacctttgc ttgtccatct gcataacgca
    30301 ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct gctaatacag
    30361 ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa aattgttcat
    30421 aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg agatccattt
    30481 ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata ctgagtaatc
    30541 ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta tcctctacct
    30601 gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga tcatgagaaa
    30661 ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt cctttttgtt
    30721 cattttcatc cacccatgta ttcagtgcat ctactaggtc cattagattc ttttctcgaa
    30781 attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg attttcttag
    30841 ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag ataacaacga
    30901 gaagcaacag ccacttaatc aaatctaaac cccactcccc acaccgtatg aatatacaca
    30961 gcatgtgcct ttgctagttt ccctcgaaga ttggaaatat gcatattcac tgttttctca
    31021 ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga aaatactttt
    31081 ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa atggacagcc
    31141 acaccattca catttacatc cctcgactct ttgtccagta agatttcctt gtaatagatg
    31201 cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat gccgattcgt
    31261 aacaacagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc tgctgccaac
    31321 aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg aacattcgat
    31381 gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat gagatctaga
    31441 aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc tgaataagca
    31501 tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aagaatgaac agcttcatcg
    31561 tcttcgataa tcaaaatagt ttccagcagg aacacctact taatgtcaga tttttggaaa
    31621 ataaacattc ctagagctga tgataccacg attgttacta gactgaccag aatcattttc
    31681 ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca cgtttccgta
    31741 aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg aacgaaggcc
    31801 gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct agtgatgaat
    31861 gtcaaagcca ctggaacaga taatgtcgct aatatcagca gaagcaatac aggaaatact
    31921 tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc gaaagcaagt
    31981 gtgtaagcaa tcatggctag ccccgtaatc aaaaccacgc caagtgttgt gatcaaaagc
    32041 gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga taacgtattg
    32101 cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc tgcgaaagcc
    32161 agaagaatcc atgcagccat tttttccacc attaaaatcc ctgttgcacc gttgatgact
    32221 gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca ttcgttccaa
    32281 gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt acggctcttc
    32341 catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc cagttaattg
    32401 taagaaataa tcttccagtt tttgcccaac taggtggata ccatccacag gaacttttgc
    32461 ctctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa gctggataac
    32521 acggctagat tggacaaaat aatccctata accaagagag tcgagaaccg ttactgcttg
    32581 ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca attcttcttt
    32641 cgataattct ttgatcaagt gtccttcatg caaaataccg tagtgagtgg cgatctgtga
    32701 caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt ttaccaaacg
    32761 ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg tcggttcatc
    32821 caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac gctggcgcat
    32881 acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca tcattttcag
    32941 caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac gtaggttatc
    33001 tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca tacgtcgtct
    33061 tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa tcgagatcaa
    33121 acccataata gttctcataa aggtagactt acccgctcca ttctccccaa tcaaaccata
    33181 gatcattcca cgtttgattt cgatcgatac tttatctaat gctttggcag caccgtattt
    33241 tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac ttatccttct
    33301 ttcttattga taagtgaagt atagttgacg ggtataaaga aagcagccag aaatgtataa
    33361 agaaagtgta aattttccaa ttgaatttca agaaaaatca gactgtctcg cagacgtggt
    33421 tgagttgtct gccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc
    33481 atggtaatcg ttgttatcgt tgacatcaga cgtccttgta tatgcgttgg aacttctcgc
    33541 tgaagccaga ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa
    33601 aaataataca tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag
    33661 acttttcaca atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    33721 gtcacaatat ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag
    33781 ctgaccactt ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat
    33841 ctttttcccg tttgtttata gatcgaatga tattatagac caagaatcct acctattaac
    33901 atagctaaaa acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga
    33961 ttaaggtaac tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg
    34021 aacgataatc gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct
    34081 ggttttttct tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg
    34141 taaatatatt ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta
    34201 atttaaaggc agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat
    34261 ccattctatt cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga
    34321 gcatcttatt tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa
    34381 aagaaattat gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt
    34441 gctcctacga ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta
    34501 gatttagcac atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa
    34561 gtcactgttc cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa
    34621 ccaggagtcc cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat
    34681 atgtccgata aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat
    34741 actcatattg tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca
    34801 ataaaacgat cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa
    34861 gccattgacg attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta
    34921 gggcaatcga ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt
    34981 gtggaaaatg ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca
    35041 aatgccaatt caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca
    35101 cctgtcgcct ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct
    35161 aatacctaca caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc
    35221 gataaagaat cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact
    35281 ttacctgcaa aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct
    35341 catggtacaa aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc
    35401 acccccatga ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca
    35461 cgggaaaaag ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg
    35521 aatgacaatt attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt
    35581 gaaatcgttc aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt
    35641 gctaatgcgt tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc
    35701 gtgaatacgt tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagccctt
    35761 gttaccagta acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa
    35821 gaagacaaag atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac
    35881 accttgttta ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag
    35941 ttagcgaagg gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac
    36001 caagttgccg ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc
    36061 gaaggatata gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc
    36121 gaaaaaaatg ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc
    36181 tttgatttct ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat
    36241 ctgaccttta aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa
    36301 gcgaccacag cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt
    36361 ccttatgggg attatttact tgaagaagta gaagccccag aaggttttca aaagattacg
    36421 ccattagaaa tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat
    36481 gtctttacca ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac
    36541 gagaagctga cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg
    36601 cctgaggaag aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta
    36661 acttctctgg attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa
    36721 gaagactggt atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa
    36781 aaagatgaaa aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa
    36841 gagaaaacag gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa
    36901 actattgtct tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag
    36961 ccagtagcga aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa
    37021 caccatgttt ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat
    37081 ggtgatgtgg tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca
    37141 aaagaagctt tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt
    37201 tggaaatctg gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt
    37261 gcgaaaaaag tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc
    37321 aattacgata aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct
    37381 caaaccttaa caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca
    37441 ccaactgttc caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc
    37501 ggggagaaaa attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg
    37561 ggctattatt tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag
    37621 aaccaacgtg cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta
    37681 acaatgaaaa gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac
    37741 ctttggaaag ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa
    37801 tactacggtg attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat
    37861 tgaggtggta gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa
    37921 agccgtgaat ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga
    37981 ttacgtgttg tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac
    38041 agaaggtatt gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg
    38101 ccgagaagta cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac
    38161 ctataattta aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt
    38221 acccttacgc gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac
    38281 cgttgccaat tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga
    38341 tttagtcttg aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa
    38401 ataggtgaag attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg
    38461 aaaaggcgag ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt
    38521 tctgattata catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt
    38581 actagactgg ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt
    38641 atgtcggcag gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga
    38701 aggcttttta aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac
    38761 tagcataact ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg
    38821 aaaccatcaa aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc
    38881 actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc
    38941 aagagtttag acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt
    39001 ctataaaaga tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc
    39061 tttatattca cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc
    39121 ggatgattct agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca
    39181 aagatttgaa tagtagccga accagagaga ctatcagtta cttccccaaa atctattatc
    39241 gaatgaaaga tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc
    39301 agcttttaaa attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg
    39361 aactcaaaga ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg
    39421 ggatagacga agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg
    39481 cttatgattc tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt
    39541 tcctcctcac cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc
    39601 ccaaaaatgc agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg
    39661 aagaaatttc tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa
    39721 tgaaagaaat gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca
    39781 actttttaat ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg
    39841 cggtgatttt gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc
    39901 tgattttggc ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatca
//
LOCUS       DACCDF010000017        39751 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCDF010000017 DACCDF010000000
VERSION     DACCDF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.017"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            33..1082
                     /locus_tag="IVK51_000882"
     CDS             33..1082
                     /locus_tag="IVK51_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430494.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1079..1960
                     /locus_tag="IVK51_000883"
     CDS             1079..1960
                     /locus_tag="IVK51_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430495.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            1963..2733
                     /locus_tag="IVK51_000884"
     CDS             1963..2733
                     /locus_tag="IVK51_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430496.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3012..3866
                     /gene="aroE"
                     /locus_tag="IVK51_000885"
     CDS             3012..3866
                     /gene="aroE"
                     /locus_tag="IVK51_000885"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP6430497.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4044..5207
                     /locus_tag="IVK51_000886"
     CDS             4044..5207
                     /locus_tag="IVK51_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP6430498.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5324..5734
                     /locus_tag="IVK51_000887"
     CDS             5324..5734
                     /locus_tag="IVK51_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP6430499.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            5908..8136
                     /gene="topB"
                     /locus_tag="IVK51_000888"
     CDS             5908..8136
                     /gene="topB"
                     /locus_tag="IVK51_000888"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6430500.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8274..8591
                     /locus_tag="IVK51_000889"
     CDS             8274..8591
                     /locus_tag="IVK51_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430501.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            8751..8987
                     /locus_tag="IVK51_000890"
     CDS             8751..8987
                     /locus_tag="IVK51_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6430502.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            8984..9382
                     /locus_tag="IVK51_000891"
     CDS             8984..9382
                     /locus_tag="IVK51_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP6430503.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(10049..10993)
                     /locus_tag="IVK51_000892"
     CDS             complement(10049..10993)
                     /locus_tag="IVK51_000892"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6430504.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(11289..12233)
                     /locus_tag="IVK51_000893"
     CDS             complement(11289..12233)
                     /locus_tag="IVK51_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430505.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            12454..13668
                     /locus_tag="IVK51_000894"
     CDS             12454..13668
                     /locus_tag="IVK51_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6430506.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            13690..15705
                     /locus_tag="IVK51_000895"
     CDS             13690..15705
                     /locus_tag="IVK51_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP6430507.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(15788..17161)
                     /locus_tag="IVK51_000896"
     CDS             complement(15788..17161)
                     /locus_tag="IVK51_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6430508.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17187..17471)
                     /locus_tag="IVK51_000897"
     CDS             complement(17187..17471)
                     /locus_tag="IVK51_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6430509.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(17473..19488)
                     /locus_tag="IVK51_000898"
     CDS             complement(17473..19488)
                     /locus_tag="IVK51_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6430510.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(19667..19972)
                     /locus_tag="IVK51_000899"
     CDS             complement(19667..19972)
                     /locus_tag="IVK51_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP6430511.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20061..21092)
                     /locus_tag="IVK51_000900"
     CDS             complement(20061..21092)
                     /locus_tag="IVK51_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP6430512.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            21299..22174
                     /locus_tag="IVK51_000901"
     CDS             21299..22174
                     /locus_tag="IVK51_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6430513.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(22326..23168)
                     /locus_tag="IVK51_000902"
     CDS             complement(22326..23168)
                     /locus_tag="IVK51_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6430514.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(23260..25137)
                     /locus_tag="IVK51_000903"
     CDS             complement(23260..25137)
                     /locus_tag="IVK51_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6430515.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(25134..26555)
                     /locus_tag="IVK51_000904"
     CDS             complement(25134..26555)
                     /locus_tag="IVK51_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP6430516.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(26558..29947)
                     /locus_tag="IVK51_000905"
     CDS             complement(26558..29947)
                     /locus_tag="IVK51_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP6430517.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            30204..31595
                     /locus_tag="IVK51_000906"
     CDS             30204..31595
                     /locus_tag="IVK51_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6430518.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(31668..32315)
                     /locus_tag="IVK51_000907"
     CDS             complement(31668..32315)
                     /locus_tag="IVK51_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6430519.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(32323..33009)
                     /locus_tag="IVK51_000908"
     CDS             complement(32323..33009)
                     /locus_tag="IVK51_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430520.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33015..34088)
                     /locus_tag="IVK51_000909"
     CDS             complement(33015..34088)
                     /locus_tag="IVK51_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP6430521.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            34310..34984
                     /locus_tag="IVK51_000910"
     CDS             34310..34984
                     /locus_tag="IVK51_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430522.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            34989..36329
                     /locus_tag="IVK51_000911"
     CDS             34989..36329
                     /locus_tag="IVK51_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6430523.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(36478..37464)
                     /locus_tag="IVK51_000912"
     CDS             complement(36478..37464)
                     /locus_tag="IVK51_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP6430524.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            38188..39219
                     /locus_tag="IVK51_000913"
     CDS             38188..39219
                     /locus_tag="IVK51_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6430525.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            39328..>39751
                     /locus_tag="IVK51_000914"
     CDS             39328..>39751
                     /locus_tag="IVK51_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6430526.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT        12299 a         7252 c         7446 g        12754 t
ORIGIN      
        1 aataaaaaga ttgactttgc aggaggaaaa aaatgaaaaa caaacgatta ataacagttg
       61 tagtaattat tttagtttat ttagcaggtg catttattta tggaaaagcc tcaggcgaaa
      121 aagaaagtac ttcgacgaac cagacaaaaa aaacaacagt tgttggggta ttgcaatacg
      181 tgagccatcc ggcattagat gagatttatc gaggaatcaa agatggtctg aaagaagaag
      241 gattaaaaga aggagaaaat cttgagatca aatttcaaaa tggacaagcc gatcagagca
      301 agctagccac aatgagtcaa cagctagtac aatctgatcc agatgccttg attggaatcg
      361 caacacctgc cgctcaatca ttagcaaaca cgacttcatc gatcccagtt gtgttaggtg
      421 ctgtaacgga tcctgtgggg gctggattag tcgcttcttt agagaaacct ggtggtaatg
      481 taacaggtgt ttctgatcag ccgcctgtta cttcacaaat caagcttgct tctgatttgt
      541 tgccaaatgc aaaaaaagtc ggaatcttgt atgcttcttc agaggataat tcaaaatacc
      601 aagtagcaca agcagaagaa gctgcaaaaa aagaagggtt agaatcagta aaatatgcgg
      661 tgccttccac taatgagatt gcacaaacgg ttcaagtgat gagccgacaa gtcgatttta
      721 tctatgttcc attagataat accattgcca atgcgatgca aacagtggta aaagaagcaa
      781 acaaagcaaa tatccccgtc attccatctg tggatacaat ggtggaacaa ggtggactag
      841 caacaatcgg gatcaatcaa tatcaattag ggcttcaaag cggtaagatg gcggcagaac
      901 ttgcgagtgg aaaagaaaaa ccggaaacga cgcctgttta tacatttgat caaggggata
      961 cagtgatcaa tcaatctcaa gctgatcgct taggaatcat tgttccccaa tcgatgaagg
     1021 aaaaagccaa aatcattaca gatgactcgc agcaagaaac atctaaggag gatgacaaat
     1081 gatcgtatca gctatttcac aaggcatgct ttgggcaata ttaggattag ggattttcat
     1141 gacttatcgg atcttagact ttcctgatat gacgacagaa gggtccttcc cattaggtgc
     1201 tgctgtctgt gtgacagcta ttactcatgg ggtagcgccg attgtagcta cattattagg
     1261 cgtaggtgct ggcatgatag caggactcgt tacggggtta ctttatacaa aaggaaagat
     1321 accggttatt ttagcaggga ttttagtgat gtcaggattg aactcagtta ttttgtttgt
     1381 gatgcgaaca ccaaatcttt ctttattgaa tcatccgatt ctacaagaca gcttccatcg
     1441 ctttgggtta cctgattact ttgacacagt ccttcttggc ttcatctctt tgggcatctt
     1501 gattgctgta ctgctattct tcttcaatac agatttgggt caaggctata ttgcgacagg
     1561 agacaacgaa acgatggctc ggtcgttagg gataaaaacc gatcgtatga aaattttagg
     1621 tttaactttg tcgaacggtg tcattgcatt atccggtgcg ctgattgccc agaacgacgg
     1681 atatgcagat gtaaataaag ggattggcgt aatcgtcatc ggtcttgctt ccatcatcat
     1741 cggtgaagtg atcttccaag aattgacgct agcagaaaga ttgattgcta ttgttgtcgg
     1801 cagtatcatt tatcagctat tgattttagt agtaatcaaa ctagggtttg acacgactta
     1861 tctgaagttg ttctccgcta tcatcttagc tatttgtctg atgatcccgc aattgaagaa
     1921 agcactgaac ttaaaaacgg gttttgagaa ggaggcatag taatgactga tatattgaca
     1981 atcaaaaatg gcagaaaagt cgtaaacaat ggaatgaatg aagagaaagt actattggat
     2041 gaaattaacc ttacaatcag agaaggggac ttcatcactg tccttggcgg gaatggtgct
     2101 ggaaaaagca cactgttcaa tacgatcgct ggtacgcttc aactcagtca aggatcgatc
     2161 tcttttaaag atcagctgat cacaaaagat tcagaagaaa aaagagcacg attcctttct
     2221 cgagttttcc aagaccccaa gatgggaacg gctcctcgga tgacagtagc agaaaatttg
     2281 ctacttgcac aaaaaagagg tcaaaaacgg aaattccgtt tacgtaactt gaaaaatcaa
     2341 aaagaagatt ttttcagact atgtcaagaa gtcggtaatg gcctagagca acacttggat
     2401 actccagcgg gtgaattatc tggtggtcaa cgccaagcat taagcttgct gatggcgacg
     2461 atcacagctc ctgagttact attattagat gaacatactg cagctttaga tcctaaaaca
     2521 gctaaagcat taatgcgatt aacaaatcag cggatcaatg aacaaaaatt gacttgtctc
     2581 atgatcacgc atcgaatgga agacgccttg aaatacggaa atcgtctgat cgtactccaa
     2641 aaagggaaaa tcgttcaaga tatttctgaa aaagaaaagc aacatttgac aatgggcgac
     2701 cttttgcagt tctttgaaga agtagtggat tgatttcgac agaaaaattc ctttcagtag
     2761 aaggacttga aaactcattg acatcttcct ttgattcatc ctatacttaa aacaataaac
     2821 aattaaacat atttgggtgt tttgtaggat cacgtagtca atgattttaa caattaaata
     2881 gaatattctt tgctctgaca agtaaacgat gagaaaagaa tttgctactt tgccacctag
     2941 acgtttcgta ctttttcgta tcaaacgcta agtggctttt tttattgaag taagaacaaa
     3001 ggaggaataa gatgatttct ggacaaacca aattagctgg gctctttgct gatccggctt
     3061 ctcacagttt atccccattg atgcacaata ctgcttttga agcaaacaac atcgatgcgg
     3121 tgtatttagc tttccaagta aatccaatga atttgaagca agcagtcgaa agtattcgaa
     3181 cttttgatat gttaggtgta aatctttcga tgccgaataa aacagcggtt attccttatc
     3241 tggatgaggt aagccaggaa gcgcaattga tcggagcagt gaatacgatc gtgcacgaaa
     3301 agggtcgttt gattggctat aatactgatg gtgcgggctt catgcaatcc gtgcaagaag
     3361 ctgggatttc gatcaaaaag aaaaaaatga ctctactggg agctggaggt gcagcaaagg
     3421 cagtcactgt tcaagcagcg ttagacggcg ctgaggaaat cgtagtttat aaacgaaaca
     3481 accaaacatt cgctcaagta aaagaaacat tcgaacaact cagctatcga acttcttgta
     3541 agataaaagt tcgtgacttt gccgatacgc atcaattaag aaaagacttg aaagaaagct
     3601 gtcttctagt caatgcaaca gatatcggga tgggaaagaa aatcaatcag tcaccgctct
     3661 tagatataag catgatgcat gagaatctag cggtttttga tttgatttat gcacctagag
     3721 aaacacgttt attaaaagaa gcaaaaaaag taggagcaaa aggatacaat ggacttggca
     3781 tgttgatcta ccaaggagca atcgcgtttg aattatggac aaaggaaaag atgccgatcc
     3841 atgtgattga aaagctgttt gattaaaaaa ggataataga taaagatctt ttattctcgc
     3901 ataaacggtc tcaagaaaag taatccttta tgtatcgaag catttatttc gttatttttt
     3961 attaaattat caaaaaatga taggcttttg tcgattggtt atcgtatcct ttatttatca
     4021 gtaagaaacg aggaggaacc acaatgagtc ggatggatcg ctataaggac atccataaga
     4081 aggcgaaacc tctgaagcgg gatacgggga gtatttttgc tagaagagaa cgaaaacgaa
     4141 cgcgtatgga aaatgaggaa gatgctaccc gtatttatga aagagagcag cattcatttg
     4201 aacatttgaa ccaatacgag caacagatat acagagatgc accaagagca gaagaacaac
     4261 aaaaaaataa aaaatttttc aatcggcaaa agaagaacaa accgttaaaa cctcgaagaa
     4321 agcgatcgtg gaaaaaaatc attctatcga ttttgcttgt tttgatcctt tattcaggta
     4381 tctcattttt tattggtaaa tatgtagctg aacatgacac aagcttagag gcagcgactt
     4441 cagaaacctt tcatgggcaa aaagcgacga atggtgcgca taatattttg atccttggaa
     4501 gtgatacacg cggagaagat gccgggcgag ccgatacgat catggtctta cagttggatg
     4561 gtccttccca taagccgaaa ctgatttctt ttatgcgtga cagcttcgtt acgatcccag
     4621 gggtaggaca aaataagatc aattcagctt atgcatacgg aggagccgat ctagtgcgtc
     4681 aaactttagt agaaaatttt ggaatcgatt gtcaatacta tgcgaaagtc gatttcaaat
     4741 cgtttgaaaa agtgatcgat gccttattca tgaacggagt gaagatcgat gcagaaaaag
     4801 atctgaatct ggatggtgta gatattaaaa aagggggcca gaagatggat ggacatgttc
     4861 ttttgcagta cgcacgtttc cgaatggatg aacaaggaga ctttggccgt gtcagacgcc
     4921 aacagcaagt aatgaacgct attttcagcc aattgaagaa tccattgaat ctgatccgtg
     4981 caccttatgc agctggtaaa gctttaggat atacatccac agatgtcact gcttggttct
     5041 tgacgaaaaa cctcttttcg attgcacgag gagctggtgg cattgatcga ataagtgtgc
     5101 cagtagacgg ttcgtggaat tatgggcaaa gtgattatgc tggcagtatt cttgaaattg
     5161 ataatcaaat gaatcaatct gctattacag attttttgaa taaatgatca taaaaaagat
     5221 caagaagaag ctgcgcaagg cttttgtcat gaatcaatca aacgattcac tcgtaaaaaa
     5281 cgctttctta tggtagattt aaccaagaga ggagctgttt acattggata tctatctgaa
     5341 cttcaaaaat gagtcgcgtg aagccatcgc tttttatgaa aaagtttttc agactgaagc
     5401 atcaggtatc accacatacg gtgaaatgcc tggaatagaa caaatgaact tgccagaaga
     5461 agcaaaaaat ctaatcacga atgcacggct gaacatttat gatacattag ttatgtttgc
     5521 agatacgcca gaaggaattg gtcctgaatt agttgtggga aataatgttt ctttagtcat
     5581 ccagacaaga aatgaagaag aaacgaagcg cctatttgaa agcttagctg agggtggtaa
     5641 gattgagatg ccactggatg ctgttccgtg ggctcagttg tatgggatgg taactgatcg
     5701 atttggcatc aactggcaga ttaaccaaga gtgacagcta aagatgaata ttttctggaa
     5761 acatcaagca gcaaacggat catcgtttgt ttgcttgatg tttttgtgtc tatttcgact
     5821 atacggtatc ttttttttat tgccagatgc gaaaatatgt tccataatag agaagtccgc
     5881 aaaaaattaa gtaacggagg tcagaagatg aagacggtga tactagctga aaaaccttca
     5941 caagcaaaag cctatgccga aaccttccaa cagcaaaagc ggcatgaagg gtattatgaa
     6001 atcagcgatc cattgtttgc tggtgaagta accatcactt atggttttgg acatttggta
     6061 gatatggttg ctccaggagc ttatgaagag cgttgggcaa aatggtcttt agaaaatttg
     6121 cctattttcc cagaaacctt ccgttacgaa gttccaatgg acaaacgtgc acaattttcg
     6181 atcgtaaaac gagaattgca gtcagccgat accatcatta ttgcaacaga tggtgatcga
     6241 gaaggtgaag ccatcgcgtg gtcgattatt cgacaagcaa acgcattttc taaggaaaag
     6301 aactatttac gtttatggat caattctctt gaaaaagaag caatctatga aggttttaag
     6361 aatctccaac ctggaaagaa ctattttcca aaatataaag aagcgcaagc aaggcagaac
     6421 gctgattggc tgattggaat gaatggaagt ccattatata gcttgctact tcaacgaaaa
     6481 gggattgatg gcagtttttc tcttggtcgt gtacaaacac cgacactcta tatgatttat
     6541 cagctgcaag aagaaatcaa acacttcaaa aaagaaacgt actttgaagg agaaggttta
     6601 attacgacaa acaaagggca gttttctgga aaaatcgtac caaaaaaagc atttaaaacc
     6661 caagaagagt taacgaagga aatagaaaaa cttggagcgc atttaggtaa gcaaaacgga
     6721 cggattcttg aagttacgaa aaaggaaaag aggttgcata gtccacgttt attttcgcta
     6781 tctagtttgc agacaaagat gaatcagcta atgaaagcaa gtgccaaagc tacacttgat
     6841 gctgctcaag gattgtacga aagcaagttt ctaagctatc caagaacgga ttccttttac
     6901 attacagaaa atgagcatca gtatctggtt aaacatctgc aaaattataa aaaattttta
     6961 ggaatagaag aagtacagac aactgagatc aaagcaaaga aacgatatgt cgatgcgaaa
     7021 aaagttcaag aacatcatgc aattattccg acaaagacga ttccgactcc tgcacgcttt
     7081 gcgaagcttt ccaaattaca gcaagcaatc tatttgcaag ttttgcgcac tactgtggct
     7141 atgtttgctg cagattatat ctacgaagag acgaccgtaa tgacgggcgt acagcaatta
     7201 aagctaaaaa gcatcggaaa gatcccatta cagcaaggtt ggcaggaaat cttacgcatc
     7261 aagtcaaaaa agaaagaagc tcagactctt cctcctatcg tagaaaatga agaagcagaa
     7321 gtagaactga aaagtatgga gaaagaaaca caacctccaa aaccctatac cgaaggaacg
     7381 ttgatcacag caatgaaaac tgcgggaaaa acgctggata atgatgaagc gcaagaaatt
     7441 ctcaaagaag tagaaggaat agggacagaa gctacacgag ccaatatcat tgaaaatctt
     7501 aagcagaagc attacatcga agtcaataaa aacgaaataa ctgttacgcc aaaaggaatc
     7561 actctttgta aggcagtagc caatgaacca ttactgacga gcgcagaaat gactgcgcgc
     7621 tgggaagggt acttgagaaa aattggtaaa caagagggga caccatctgt ttttttagag
     7681 aatatcaaaa aattcatcct acatttattg gcagaagttc ctaatcaaat cgacaaggta
     7741 gacttttcag atgagattag cgggacacgt cagataaaag cgatggagaa gaaaaacgat
     7801 caattaggaa gatgtccaaa atgtaaaaag ggaatagtca tgctttatcc taaaatcgct
     7861 acatgcttga atccggaatg cgacttcaaa ttgtggccaa cagtagctaa aaagaagctg
     7921 actaaaacga atctcagaga tttgctttca aaaggaaaga caagtaaagt tgtcaaaggt
     7981 tttactggta aaaaagggaa attcgatgca gttttagtat taaaagaaga tatgacaatc
     8041 ggtttttctt ttccatggat cgaaaacagt ggaacaaaag cagttgatgg aaacaaagaa
     8101 acatcaagaa aaaataatga aaacgaagta gagtaaatga aaacaatgaa atagcggaat
     8161 atttattccg aaaagtgaga atataaatac caataaaact aaaaaatatc agtgaatcaa
     8221 gcaaaaacgt tggggtaggc tgattttata tagatagcaa ggagtttttt tatttgaaaa
     8281 aagtgcattc atttttaaat tatcttatgg gagcaatcac gattgtggct gtgtaccaag
     8341 catttctaaa gtatcctgat tacgggacga agttgttgtc attgacgcac aatcaaacag
     8401 catattcacc aacgttgatt ttatcgactt tgactgcagg agctaaaagc gctacttatt
     8461 tttttcataa aacgacaagc atgctaaagc gaaagaaaca gcgaaaacaa ttgttggtgg
     8521 tggtagcggc tatgatcatt atttattttc taccagttat tacacaagta tttcatcaat
     8581 attttggtta agaatacgag agtttgtgac aaaagtcatg tcacaaactt ttttctttgt
     8641 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
     8701 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
     8761 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
     8821 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
     8881 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
     8941 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
     9001 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
     9061 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
     9121 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
     9181 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
     9241 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
     9301 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
     9361 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaatta gatcagcatg
     9421 tcattaagga atatgctgat ttttttatgt ttaatcatac aaaatagttt tttcataaaa
     9481 atatggaaaa tatggattaa ttttgaaata attgatttaa aaggaataca aaagagtgaa
     9541 tagtaggaaa tagtggttta gaactagtgg gctgattttt tatatatttt agtttacata
     9601 atatatatta tacaaagtag atttaaaaaa tgataaacct ctctcgaacg gttttttatc
     9661 aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa ttataaaaaa attttgcagt
     9721 ttttcttact ttttttgtcg tttaattgaa tgataaaaac cagtttcttt attcagttaa
     9781 tcaaatatga acgaattaga tatgaatcat tgtatttgcc gttgcggaag gattttttaa
     9841 ttaaccacag ataaacattg ataacgattc gcttattgta atcaacagat taaatttcat
     9901 atgttcatat gtacggatac attttcggac cgattaattt taaccattct gtttatttgc
     9961 ttacctatta gatatcaata aaaaactgct taacagctat ttcagctgtt aagcagtttt
    10021 aggattttta gttcatattc attgtttctt ataatacagt gttcaatact tctcttaaag
    10081 cacttgttga tttgctgaac attgcttttt cttcttcgtt gattgataat tcaacaactt
    10141 cacgaacacc attttcgtca acgattgatg gcacacctgt aaagatatct ttttcgtcgt
    10201 attcaccagt taaataagca gatacaggca atacagcttg ttcgttattc aagatagctt
    10261 ttacgatacg tgcagtactc ataccaatac catagtaagt tgctttttta cgatcaatga
    10321 tctcataagc agcattacgg actttatcag caatcacatc taattcatct tgggcaatac
    10381 gatggtcttt ttctacaatt tcaaacactg gtttaccacc gacagttgta tgtgaccatg
    10441 cagcaacttc tgaatcacca tgttcaccta agatatagcc gtggacgctg cgtgggtcaa
    10501 cttttaattt caatgcgatt tctttacgga aacgagttgt atccaatgtt gttccagtac
    10561 cgataacacg tgatgttggc agaccagatt cgttccaagc gatatatgtc aaaatatcta
    10621 ctgggtttga agcaacaacg ataataccgt cgaatcctga tcccatgatt tcttttacga
    10681 tttggcgcat gattgacgca tttgttttaa ctaaatctaa acgagattga ccaggttttt
    10741 ggttgatacc agctgtgata acaacgatat cagcatcttt acattcttca tagccacctg
    10801 accatacagc cacattttca tcgccccaag ccataccatc taaaaggtcc aatgcttccc
    10861 cttctgcttt ttcttgattc acatcgatca atactaattc attagagatt ccttggttga
    10921 tcattgcata agcgatactt gttccaacaa aacctgtacc gacgattact actttacgac
    10981 ttgttttttt catgaataaa cagctccttt tcataactat ttgttacttt catgaaaagt
    11041 atagtcttgt ttattcattt tatcaacatg ttaattataa tagttgttat tataattttt
    11101 gatggaaacg aattcctgaa tacgcataac agataaatga caaatgttta cacctatatc
    11161 ccctgataat acaaccaaaa gtttagaact gaaaataaat ccaaaaaaat tcgctgcaac
    11221 gatataataa gacagcaatc gcacctaaaa caattttcct tcatatgctt tagtgaaaga
    11281 ttttcttctt aatgtttatt tctccattca ttgggagtac atgttgaata cttcttgaat
    11341 aatttgaata aagaagtcgt ttgtttatat ccgatacgat tagcgatttc ttccaacgaa
    11401 aaactcgttt cttgaatcag ttccaacgca aagtcgaatt ttttctttag aaccagttct
    11461 tgaaaggaag catcaaaata cattttcaat ttattactaa gatagaccgg attgtagcca
    11521 aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat gcttatcgat ataatctaat
    11581 actctaagca tctcattttc tgcgcatcca tctgctgatg aaaagctttg ttgttctaat
    11641 aaatgagtca attcgatcag taatagtgat gtatagagat ttaacgaacg gatcttatga
    11701 ctgtatgtat caaaataaat caacagcata ttttcaatca aatgcgtcaa acgacttgtt
    11761 tttccgccgc gaaaatgaat gaattgtgta tgttcccctt tttcttttga tgcatttatc
    11821 aaaaaagaag aaagtagatt atttgtgctt gccaaatgat tcaatatctc tgtagataag
    11881 gtttcttctt ttaataagat gttgattaaa atatcctctt gccctgtgta ttcgattctt
    11941 tgagtagctt ctttatcaat gatcaccacg tcactttctt ccatctctat cctttctcca
    12001 ttaataattt gtaaacatgt accgctatac atataattga tttcaacaaa atcatgtgta
    12061 tgctcgggaa cttccgcata tcgacggtgt ttactgatat gaatatttcc ctgttgaaat
    12121 agatcccctc ggtggatttt tggcggtgtc tgaagatggt aagctaagtt taaatcattg
    12181 atattcattc cttgttcacg ctgcagagtt tcgattgatt cttcgttacg caaaaaaaat
    12241 gtctaattca gctagttcca attgactccc tcctttttgt aagcggtttc tttcgcttat
    12301 tagttcacgc tgtactgcta ttatatgcaa agctattttt ggtttcataa agactttcta
    12361 tggttgggac atgaacttcc tgtgattccg ctattgagag aataaccact tcatactata
    12421 tttttgataa gcgcttacaa aaatgaaagg aacatgctta tgaataaaaa gatgatgaaa
    12481 ttttctctgc tgtcagtctc tcttattttg acttctgctg gtgcagtcac gtctgcaatt
    12541 cccagtatgt cagctactta tgccacgcgt tctttatctg ctgttgaatt attaacgact
    12601 gctccctctt ttatggtcac tatttttgtt ttgattagta gttttattgc taggaaaatc
    12661 ggacaaaaac aaactgtgat catcggatta gtgattgcat cgatttgtgg tatcctcccc
    12721 ctcttctcac aaagttacag tattgtctta gcttcaagag ctggactagg tattggcttt
    12781 ggattgatca attctttggc agtcagcatg attgctgcct tttatcacgg agatgaacga
    12841 gcctctatga ttggttttca aagtgcattt caaggatttg gtgcagcttt aatgacattt
    12901 gttgcaggac agctagtaca gttcggttgg caatatacct ttttagtcta cgccatcagc
    12961 ttacctattc ttgtcttgtt tgttttgttt gttcctacac ctgaaacaac tgaacttcaa
    13021 tcgcatggca cagccccaac aagacaagta atcaaaacga atcctaaaat gattagttat
    13081 ggtatttttc tcttgctggt catcgtggta tataatgctg tatcgattaa attagccaca
    13141 gtcctgacga cttattctct aggaaacgaa actaatgcag cgaatatctt aacaatcatg
    13201 caattggcaa gtatggctgc cggagtttgt tttggctggc ttcagacaaa agcacatcgc
    13261 tatatgctta cactttcttt actactgatg ggaatcggct ttgtattgat tgccactgtg
    13321 ataaacctgt atgtgattgg gtttggtgcg atcctaacag gaatttcttt ttcgttattc
    13381 attccttact tattcaacca agtaagtatc caagcgccca agtacgcgga gagtttcaat
    13441 acttcaatct tattggttgg cgctaattta ggtactttca ttgcaccata tggtttgctc
    13501 tttctctctt ttgcttctct tgggagtaaa acacttgctg ttttcgtcaa tggcagtatc
    13561 ttgctgttga ttagcgcgct tattagcctt ctagtagtac acaggacgaa tagaaagaca
    13621 gtgataagag tagatgaaga aataaaagaa caattgacaa caaaataaat ttgatggagg
    13681 tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc tttggtatcc tggagacttt
    13741 gagatccgcc atggactgct acaaaatttc caacgggaag aacgaggttt tgactggcca
    13801 gcgtactggt acatggatga ttgccatcgg aacgtcaagt ttaagagata ttactttctt
    13861 gatcaacctt ccatgtttaa agtgacgatc caaggagttg ggtatgtaga aattaatgga
    13921 caaaaacatc catgtggaaa atggctgact tgtccagctg gtaaagcaaa aattcgtatc
    13981 tttgtaggac atacatcagg gctaccagcg atgtttatcg agggagacga agtaaaatca
    14041 gatattggct ggacagcaag taattttatt gaagaatatc cagctggctg gagtccgctt
    14101 tatgtcgata tcgcaaaaga tccaaatcaa atctattatc aaaaagaatg cgtccatcca
    14161 gtcactgaaa cggagattgg cggaggaatc ttattcgatt ttgggcgagc agtgaatggc
    14221 tgtgtagaac taatatctct ggatgaccat atcgaatcta tcactctatg ttatggagag
    14281 tccgatgtag aagctatgga tatcgattat tgctactata agcaaatagg aatgacaaga
    14341 aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta taccagaggt ttctactgga
    14401 atggttcaac ttcaagtttt tcatgaattt ttatctcttt cgaaacaagc tgctttttca
    14461 agcgattctt ctttactgaa tcagatctgg tcagtatcag aagaagcctt caagttatgt
    14521 agtgatttgt tttttatcga cggtatcaaa cgagatcgct ggatttggtc tggagatgcg
    14581 tatcaatcat tcatgatcaa tcaatatctt ttttttgacg aggaaatcaa taaacgaacg
    14641 atattagcat taagaggaca aaatgaaatc aagcagcata tgaatactat cgttgactat
    14701 tcgatcttgt ggttgattgg tatcgaaaac cactatatga tgacgaaaga ctatgaattt
    14761 ttacgcatcg tctaccctaa gatgcaatca ttaatgagat acttgatgga gcaaacgaat
    14821 gaattagggt ttatttatgg gagagcaaaa gattggatat ttattgattg gtctgaaatg
    14881 gataaagaag gaacgcttgc tgcagaacaa gtcttattac tgaaagccta tcattccatg
    14941 atcacttgcg gagatatctt agctgaaaca tatggagaga acgaaaatta tcatactcat
    15001 ctctatcaag aaaaatatca actattgaaa caaaacctat ttgactactt ttgggatgaa
    15061 gaacttggcg cttttatcga ttgctatgaa tctgggagaa gaaatgtgac gcgtcatgcg
    15121 aatattttcg ctgttttatt cgattttgct aacaaagacc aacagcaatc gatcttaaag
    15181 aatgtcttat tgaatgacac tatcacacag attactactc catatttcaa attctatgaa
    15241 caagatgctt tatgcaaact cggacagacg aatatcgtct accaaacgat tcttgattat
    15301 tggggaggga tgttatctca tgatgcggta actttctggg aagaatacga ccctactcaa
    15361 acaggaaatg aaaagtacag tatgtatgga gatccttatg gaaaaagctt atgccatgca
    15421 tggggagcaa gcccaatcta tcttttagga cgctatttta tcggtttgta tccaacaaag
    15481 ccaggttatg aagaatttat gatacaacct aatctagatt catttgaaac actgaattgt
    15541 acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa caaaagataa gatcgtgatt
    15601 acttctgata gaacaggcgg aacagtgaaa atgaatggta aaagttatcc tcttctagca
    15661 aaacagccac ttgtcttaac aagctattca aaaataaatg cataaaacaa aaaactgctt
    15721 gcgacatatc agaagacttt aaatcttctt ctgatattgt ccaagcaatt tttattttga
    15781 agtttgctta attctgttct ttagaaacaa tgtttgttct ttttccggca aaaagaaagc
    15841 tagataatac gacaagtaaa aagaaagcga tgattccaac tgtcaaggct atttgaccgt
    15901 attctcctag tttcccaagt acaagtcctg gtactagata atcagtatcg gcaaatgtag
    15961 aagaagcgat tttaagatct cctaatactg gaaggataaa taatggcagc caactaatca
    16021 ataagctatt gaaagcagaa cctaataccg cccctcttct accgccagtc gagttgccga
    16081 agacaccaga agcagctccg cagaagaaat gtcctactac cccaggaatg ataactgtag
    16141 tttgcaagcc tatcatagca aacatggata gtgttcctac aaagaagcta acaaaaaagc
    16201 caattaatac agcattaggt gcatatggaa aaacgatagg aacatctaat gctggtttag
    16261 aatcgggtac gagtttttta gcaatccctt ggaatgctgg aacgatttct gccaacacca
    16321 ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa tgttccagct tggatcagtg
    16381 caaagattac agcgttgtct ccattgctta gttctctttc aacaaatcca ggaccagcta
    16441 acaaagcaag tgcgacatat acgatcgaca tcaagagcat gatcgataca gtagaatctc
    16501 gcaaaaagct gaaactttta ggaatcttta tgtcctctgt agaacggtct ttgtttccaa
    16561 aaaactttcc gatccatcca ctagaagcat aaccgatatt tccggtatgc cctaacgcaa
    16621 cttgattgtt tccagtgatg ttcttcataa acggttgaca aagagcaggc gtaatagtta
    16681 atagtgttcc ttcgaagacc ccgcctaata agatcatcca caaatttgca gtaaaccccg
    16741 cgcttactaa aatgactgct gttagacaag aaacataaag cattgcttga cctgtaagaa
    16801 aaatgaattt atatcttgtg aatctagcaa gtaaaatatt cacgatcatc cctactagca
    16861 tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat cgccactact gcttcattgc
    16921 taggaactac tccttgagta tgcagagctg tttggaacat cgaagcaaaa ggggttagtg
    16981 aattactgat taaaccagca ccgccgatca gtactaggaa tccgacaaat gttttgatag
    17041 ttccttggat cacttctgaa aatttcttct tctgaagaac gagaccaagc agtgcaatca
    17101 aagaaacgag gatggagggc tgactgacga ttgacacaaa caaattcaac aatttatcca
    17161 ttgatagttc ctcccttgaa tcatttttat tcttttgaca agatcttcgt tactttttct
    17221 tttacttctt cgctgtcaat gatcgattgc aaaggtacga gcttttcatc aggtatagaa
    17281 gttagcgtat ctttcaaaga atgctcaatg aagaaataat cagccatatc gctagataca
    17341 cttccaacat ccgtatgact tacttcaaac tcgtccacat ctgctccaag atctgcaagt
    17401 gcttgtttga tgttcatctc taccataaaa cttgttccta atccactttg acatactgca
    17461 aaaattttca tatcattcgt ctccttttct tatgatcgct aatatttctt cttttgttga
    17521 tgcgtgtaat aaatcttgaa gattttgttt attagaaagt agctttgtta aatttgccaa
    17581 agcccgaaga tgtgcctcat tatcgatagc agctaaacag atgaacaaac taatgggatg
    17641 ggctggatta tcaagcagat ccacaggttg ttgaagtttc agcaaagaca tccctacttc
    17701 gtttacgcca tcttctggac gagcatgagg taatgcgatg tgatcgccaa tatggataaa
    17761 cgcaccatac tgctccacgc gctcgatcat agctttgata taactttctt ctattttatt
    17821 ttctgataat agcggctgtg cggcaaatgt gattgcttct tgccagtttt ttggttgatc
    17881 cgataattgg atcatttctt ctgttaataa ttcagatagc ataggacgtt gatcctcctt
    17941 cttttctaaa agtttgttca ttttacgatt taatacttga tagagctttt cttcgtctac
    18001 gccttctttt aattcgatat aaggcagtag cgccgacaac aattcttggg cagaaggcat
    18061 tgaaaatcct ggtatcagcc aatctctttg tacttgattg atcagctgat ttttttccaa
    18121 gtcagtcatg attggatgca gaacatagag agactttttc ttatcagtaa gtgtgagagg
    18181 tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc tcgtgtaagc ggtcagctga
    18241 attagtctct gtaaaaaaga tcatcgggaa taattgctga agttcacttt ttaagatcaa
    18301 tgaagagcta atgccacttg gacagacgat cacggctctt atttttctat ttctttcttc
    18361 tgcgtccgct tttctgatct ctccgccaaa tagtatagtg aaaaaaccaa tctcttcttc
    18421 agggatgctc ttccctattc gtctttctaa cggaagtaat gcttttcttg tcatttcgta
    18481 gatggaagca tattgatgct taatttgatc gatcatcaca ttaggtaaat aaaaagaata
    18541 ggaaatacga aaataggcag ggaccaagtg ataaaagaca ttcatcaata attcacgaaa
    18601 cgaagcaaat tctacggcca tcaggaattc catgcgatgg atgacttctg aagaacattc
    18661 cagcaaaaaa tcaagtgcag gctcctggac atctccttca acgatcgatg ctaagcaaag
    18721 agagaaaaat ataatctccg aattttgatg ttcggaatag ccaaggccag tcaatacttg
    18781 tcgactaatg tcagtgactg caagagaagc caacagttca gcatctttta ccagttccat
    18841 ggatgttttt ccaattcgaa tggttgtcaa actcaaataa taagctaatt catccattcg
    18901 atttggtgcg aatcgaagat ctgatagtga aatcgtttta tctagcaaat ctctaacttt
    18961 ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg agcccaaatc ttccagcaag
    19021 tgactgcatc aaacgagcag tgcgattttt tgcttctctt cttaatattg cttctgaccc
    19081 tagcagccta aaaccagttt tcctagtata cgacagctga caatcttcca cttctaattc
    19141 atttcttatc tttttgatat ctgacaggac tgtcccttta gaaacctgaa gaaaatcttg
    19201 aaaatgaaaa acagaaacag acgttgcagt cgaataaacc atcaaataaa gaagatctct
    19261 tcgctcttgt tcggtataca cagcttcaaa tcgagtaata tccaaaaatt tttctgccca
    19321 gtcgattgct cggaatttct ttatacggat acgggtatca tccacatatt cggcaaaagg
    19381 agttaattgt ttgatcgcct cgcttgtatt tacttcgatg atctctgctg ctttttctag
    19441 actgatttct cttggtgttt tcaggagata actaattttg tagttcacag cgctgttcac
    19501 ctcctactta tattctagta tggtagcgct tttaaatcga ggggtgtttg gtacaactta
    19561 cagttggatt ttttttacga ctgtaccgaa cgatttctat cgaatggata cagaaaaaaa
    19621 gcaggtgacc aaatgtctcc tgcttttatg aattgatttc gtccgatcaa tgatgatcat
    19681 gtttattttt tgaggtcagt tcttcaaatt tttctttgac atcttcaacc gctttttcga
    19741 tatctttttc aaagtgattg ggctcattag gctttgtttc ctcttctaca gaatttgaac
    19801 cagataaagc tgtttcttta ttttctgttt caccagagtt tgtgccagct aatgattctt
    19861 ctttgtcttc tgtttcacca gaattagttc ctgctaaagc cacttcttct tctgttttag
    19921 gggccattga acctcttaat cgtttgtgtt tttcaccgtc ataatttgtc atgttggttc
    19981 ctcctagtag ttctatactt tttcatcgta caataagaca gtttatgcgt caaaaaacaa
    20041 acccgattct tacaaatatg tcatattttt ccttctgatt cttgaatgct tttgattcct
    20101 tcttccaaaa ttttcatttg taagattgtt tcttcatctt tgatgacttt ttctgcgcca
    20161 ttaactaatg tctcgtaaat cccttcgtag acacggctgt aatcaccaac ctcggaaatt
    20221 actttttctt catgataact cccttgttca tctatatagg tcaatacacc ataatgttcg
    20281 ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa acattttcag atgttcttct
    20341 tgtcgatctt ttgtttgttt cacgaacatc cctttttttc cgtaaacgac gaaacttgga
    20401 cgttctttga tgcggaaata actggatttt atcgaaattt tcatatttcc atagaaaaaa
    20461 tccagatcga agtaatcatt cattcgtcct ttccctaaaa gttgacgtac atcatagctg
    20521 atatcatctg gttcgccgaa ataagaaaga acttgatcta ctgtatgaca agcatgtcca
    20581 tataaatagc tgttcgcagt agaaaattca tgcgtatttt ctggtacttc cggtctgaaa
    20641 taatcataat gcatttctac ttctaaaata tcccctaatt ttccgctttc gattactttt
    20701 tggactgtca agaagtcaga atcgaaccgt ctgttttggt aacattggat aaatagacct
    20761 ttttcttttg ctaatgcaaa taactcttct gcttcctttg acgtttctgc aaaaggtttc
    20821 tctactagta cattcttacc atgttcgagt gccatttttc catattcata atgaaattga
    20881 ctaggtgtag tgatgacaac taattggatc tcaggatcat cccaaagatc agacagctga
    20941 tcagtatatt cgatgtcttc gaatgttttc cacactggtt cagatgggga aaaaattttt
    21001 ttgatattga ttttgttcgt aagttttctt gaaaaaggca aatgatagcg atttgtactc
    21061 tttccattgc caagataagc gattgtaagc atgagtggtg cctcctttta ttgatgtttt
    21121 cagtatatcc tagaaaagcg cagaaagtat ttataatagc tattctggta aaaaagttca
    21181 gaaaaacact tgttatcatt cggtttggtg aaattaatgt tcaaagtaag gataaaaatc
    21241 aaaaagatag tagcggaacc aagtgtttat cagtaagata gaatttggag gtcagatcat
    21301 gcttattgaa gaaaaactaa caaaacaaga gttgtttacc acgacagaga aacggattgt
    21361 cgattacatc cgtaggaata tcgaagctgc agtctacatg acgattgaag aattagcaaa
    21421 agctacgtat acttcccatt cagctatcat tcgtttatgc aaaaaaatgg gattcagcgg
    21481 atttaaagaa ttccgtttcg agctggggcg ggaagttcat caattgattg ctcagttcaa
    21541 tcaaacgatt gatcctaatt ttcctttttc aagtgaagat agcactcagc aaattgcaaa
    21601 gaaaatggcg gatttatcga tccagtcaat aaaaaaagca caattgcaga ttgaacatga
    21661 agatttagat caaatcggta aagtattgaa caaggcacag cgaatctttc tttttgcaaa
    21721 aggtgattct caaatcacgg caaggaaatt ccagaataaa atggtcaagt tgaataagtt
    21781 tttgatcatg gctgaagaat acagtgattc ttcttggaat gcagccaatt tgactagcga
    21841 ggattgcgcc atttttatta gctacagcgg tcgcatccat cattatgaac gaataatgac
    21901 ctatttgaag catgtagagg cccctacctt gctgattacc ggaaatcagc attcagaaat
    21961 ggccaaacag gctgatatgt gtcttgttat atcgcaggag gaatatgatt ttgctaaagt
    22021 agcgactttc tcctctcaaa ttgccttcga ttacgtatta aatacgttat tctcagtgat
    22081 atattctcaa aattttgaag agaatatccg caacttgaaa aacaaacaag aattgatgca
    22141 aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag atcctcattt agtaataaga
    22201 aagatagtgt ttgataggct ctctttgata tcatcataaa aacaggctga agttttcttt
    22261 ttcaaacctc agcctgtttg tttacggaac gattcgaata gatatattat agaaatgagg
    22321 aatagctaac ttctgtttct tattttgaac gattgacgaa aaagatatat aagaaggagg
    22381 aaaatcagta tacatgcagc taacaatcca tacaggaaaa atcgtttgtt ctcggatact
    22441 ttctgtactt ctttttgaac ttcttttgca tgataaggaa cccggtgccc tctgacaagt
    22501 aagcgatgac tgttgaccat ataaggtgta caagttagga gtgtcacgaa atcctgccct
    22561 ttttcgattc tcagatcttt tgtatcagtc ggttctaccg tcttgatctg atcgacttga
    22621 taagcgagtg tccgtccatt gatttcgata tagaaagaat cacctaaagt cagtttcggt
    22681 aagtctgtga acaactttgc ttgaggcagt cctcgatggc tggaaataac ggcatgtgta
    22741 ttttctcccc ccgtaggata agacgttcct tccaataatg aggcaccttt ttctagcagc
    22801 acagtattcg tttcatcaaa aattggcaaa tggacattga tctcagggat cgtcaaaata
    22861 ccgatcgtat gtttctcaaa ataggttcta tcctttttcg caggttcttt tttcttagta
    22921 aagggatctg cacctggatt tccaccttct tttgctaatt gctggttttt ctttgtcatg
    22981 ttttcttgga ttttagccat gacttcttca ttttctttga ccgcttgttg ctgataatga
    23041 gagatgatct gctgatccag ataattattc agcgcgtcgc taacaaatgg ataagctagt
    23101 atccctattc ctgagagaag caagaaaagc atcagcagat ctagaagttt ccttttcttt
    23161 ggtttcattt gtgttctcct tgtgatgact gtttgataat aaaagaaaaa ggagcatttg
    23221 cgtaatccgc tgctcctttt tccaattctt gcaaaaagac taaatctgac tgtgcttgcg
    23281 tctagcaaag tacagtccag caatcagtag aaggactact cctgcaccga tatacacata
    23341 gattccctta ccgcctgttg aaggaagtgt acctttgtgt ttatttggta ttttttcagg
    23401 agaaatcaac tgtcctgctg ttacataaga ttgttcatcg atagtaaatg ctacacggtt
    23461 tgttaatgga acatattttt ctggcgcttt cgtttcttcc agatagtacg tgccatattt
    23521 tagacctgtc acatcgatta aaccgttact tgtggttgta aaaaccgttg ctgattcttt
    23581 cgccgatacc cagctgacgg cttttgtgga tgggtcgatc gataaatatt tcgctgtgtc
    23641 actatcttga tcacgaacga cgaattctgc tccagcaagt gtttggtctg atgtaacgtc
    23701 accatctact ttaacaaatc gtttgccccc agtaacgaca tcgactgacg gtggtgtttg
    23761 atcatttgta tgcccgttat cgacatttgc ttggttagaa aatcctttgg ttggatctgc
    23821 tttttcgttc aaatgcatat agtaaacgaa tttcaatgta ccgccaggag ttaatgaagg
    23881 aatataattc ggatcaactg aaaccgtgaa tccgtctgtt tgctcagtga cagaataatt
    23941 tactgggtcg atttctttat ttccatcata taaggcataa gcatacgttc ctgaagaatc
    24001 attatcaaat gttaaagcag cgtcatgagt atcgataagt ttgaatgttg tgtacttgtt
    24061 ttgcgttcct tctttatcag cgatacctaa tgggatattg acagaaatct catattgaat
    24121 tttttcaccg attgcgacat cttttccatt caattgaggt gttgttttat caactttaga
    24181 cgtatcattt ttgatggtct tttctactgg tgtttggcta tttgccagga tctcaaaagg
    24241 cgtgattgtt tgcttttcaa tcatttccgc attatcagga gcttttactt cttctaaatt
    24301 atattttcca acttctaaat gattaacgat caactggcct ttttcaatag atgcctcggc
    24361 aaagtcattg ttgccgatgt cataagaatg accagtaatg aaatgtttgg ctttggtttg
    24421 atcagttgtc caagtgtaca atccatctgt gacactttgg atgtattttt tgacgcttgg
    24481 tgttccttct tctttagaaa taataaattc tgctccattt agtgcttcgt tttcggcagt
    24541 accgattttt gtaactttca acgttccatc attaccgact gtatttttag ggtagagatg
    24601 gatagtatct agttcttctg tcccgtattt ataagagcca tctgcttgtt tgatcatctc
    24661 atatacaggg aaagctaaaa ccatgtttgc ggcagctgac actccgtctt ttggttcttc
    24721 tttgatcgtg tagactgcat ctttcccatt ttgtttttta ggtaaagata aagtgacatt
    24781 tccatcagca tctgtcgttc ctgaagcaac tggtgtacca ggagtcaaag actggactgc
    24841 ttgttttgct gcatccacgg acgctccttt atctcgttgc gcataaaatt cttgagtgac
    24901 gttataaact gaaaatgaaa tatcggctaa tccttggtat tgatcgaatt cgctcatttc
    24961 tttcccgctg ttttggatta aaggatcggg taaatcagtc attttctttt tatgaaggat
    25021 gacttgagct gctgtctcct ctgcaaacac tttttttgcg ccgaagctgt ttgtgagtaa
    25081 tggtaaaata aggaaaagga ctcctaacat aacgtttatt tttttatgat ttttcatttt
    25141 ttcactcctg ttcttttttt gataaacaac cagattgcaa agcttgcgaa taccatacca
    25201 acaacgccta aaagtgtatt tttcgtttcg tttgtttttg gaagaaatgg acggctattt
    25261 tcttttttat tgactacttt caatatactg tccgcctctt tatcctcaga aagaaaagtt
    25321 gaaatggtaa acggaatttc tgtttcactt aaaatataac cagaaggtgc ttcgatctct
    25381 tttaatcgat aacttcctgt tttcaacccg gaaattgaaa aagcgccatt tttatcggaa
    25441 atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt atccgttggc tgtttgctgg
    25501 aggtaatttc ctgctttgtt tttaataagg aatactgcgc cagctaatcg tttatctggt
    25561 ttatttgtct cgattttgac aaattgctta ctccctgtat atacagagac tttctttgta
    25621 ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga tttccttgtt cgcctgtgct
    25681 gtactgctta gctgcatctg ataagaaatg gtcagttttt ttccagcata tttttccaac
    25741 gaagggatcg aaaataaaac atcaaatccg tgaggtttct tgtgtatgac aaagccttct
    25801 gtcagctttt ctccattcga tgaaatcgtt aaactattag gtaaaaacgt taatgcttca
    25861 tccgcactat ctgacaattc gaacttttga tagtcaagga tatttacagg aattgtcgta
    25921 tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat ttctaggctc ttctaatacc
    25981 ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt taggatacaa atggatagtt
    26041 gaaagttttt gattgttttg tccatgtaca ggaagaacaa ctaccatatt ctctgccttt
    26101 tctttgacga cttctggtac tttggattca atgaaaagat acgctttatc tcttttttct
    26161 tgatctttag cggccagaga aaaagaagcg ataccatctt cgttgttaag agttgtagtt
    26221 gttgcttctg cggtaaacat accggaagac gcaccgtttt ttgcgatctc tgcttgtgct
    26281 tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag aatctgtgac atcataaact
    26341 tggaatgtga caccatttaa tcctcgatac gtttgtaata aagctttttc ttgtccgtca
    26401 tttggatgat ttttcggcag ttgcccgttt gggaaaagca atttgtgtaa agtaatctga
    26461 accatttctg tttctgtttc tgttgctacc tgagtaaaag ctggtttaaa tagtaacaag
    26521 aagagacaca tactaagcca accaagtttt ttcatattta gctgccccct ctttcttttt
    26581 tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat ccctactaga tagattccta
    26641 aacggccaat tcctcccgtt tcaggtaatg gtacttttgc ctgattcgtg atgtctaaag
    26701 aaatctggtt gtttttggct cctggtgaca gaacagattc atgatcttgt ccatccactt
    26761 gaattgaccc atcttcgtgt ataactatag taactggctc ttttagacct tggtatcctt
    26821 ctggtgtaaa agtttcggtc agcgtataag ttccaggttt taaattttcg aatagaaagg
    26881 tatctgtttc ttgtccatct tttggcgatt ctaagtccat ttctggtccc tgcagtctga
    26941 attttgctcc ttttaatgtc tgtccgttgt cttctttttt gtgtaccgtt agatcaaacg
    27001 cttttagtcg attttgatga gtgagcgtcc atacattatt tgtcatctcg gtattttttc
    27061 ccgtgtaaat gatttttcca tatttatcga tttggaattc ataatttccc ggcgaagtat
    27121 catatccggc aggaccagct gtttcaacga ctcgatattt accaggtgtg agtgaatcaa
    27181 aactggcaag acctttttca ttcgttgttt ggctgtcaat tggctgataa gtaccatttg
    27241 cttctttttt ctgtagggaa aatgctgctc ctttaagatt tatctctttg tccgtccctt
    27301 gcatcgcata cttacgtact gctacaggaa cttcaacaaa aggattttca atagtcaact
    27361 ggatcgtatc atcggaagta gtgactgttt ttccatcaat ggttaccgta ccatcagaag
    27421 cgatcttgat ttcccaagta gtctttttgc ttaatccatg cccttctgga gcttttgttt
    27481 ctgtcagcgt ataggtcatt tctttttgca atttgacatt ttctggaaga gaataggttc
    27541 cgtcgccatg atctgttagt aaagtatcaa tagaatctcc tgttaatttg aaaacagcgc
    27601 caataagatc cttttcacct gtagaggaat ttttcgttat tttcaagttt aacggtgtga
    27661 attgtttagt atttttccat gtcattgcat ctatttgttg agaatcgtat cctggtacag
    27721 gtaattcttt gattgtttgg taactgaatg cttgaccttg attattgtat tgaggtagtg
    27781 ataatatctc ttgataactt tctccgctat ttgcagataa tttgtcaatg tctgcacgtt
    27841 cccacgtatt tgttgtatct ttagctggtt taatgattcg aatatatccg tttttccaag
    27901 ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt aacttgatct ggacgatcag
    27961 ctagattgtt gtcaaattct tcccataact ttttgatgtg aagactgact ccgggagctt
    28021 tagcagatgg tataccgaat tcagctaatt cattggtatc aatgtttggc tggaaagttg
    28081 tcctgccgtt catttgatac cagaaatttg gatggaagtc ctcattttct gtttggattc
    28141 tcacttggta atggatttgg atttcttggt cttttcctaa atagatctga ttgctcttga
    28201 tggtatttcc ttctatggaa atagatggag tgaccgttgt aggacttgtc cccacactct
    28261 tgactgaaag tgtaccaggc tgataaatga atggttctgc gattggatca tttatttgtc
    28321 cattgcttac agttgctgag atctgtacag cttttttggc taggtattca gagatatctg
    28381 ttgcatgatc agctgattca tagtaaagat cgcctttttc atctgatgaa accatttgtc
    28441 gcatacgaga ctctacttct gcttttgaga gaccagctgc cggatcgctt tgaagttgga
    28501 tgccaagacc atgtatttcg attcctcgtt ctttgagtgc catcgcttct ccgatggttg
    28561 cgataaacgt actgtcgatt cttctggata gattgttttg gtcaggtgca tcatagattc
    28621 ttgagattag agaagtattt cccggccgat cttgcgtatt agaaaactga gttccgtaat
    28681 aattgctgct tgattgtgcg tgtacccgct gtactttata ggaaaatgtt ggtacaccat
    28741 ccgtcagcaa aacgatcact tttttatgtc catttggaac ggatagcatg cttcctgcat
    28801 ctcttagtgc tttttgtgta aaagtaccac catttgtccg tgaaggtgta atggatttta
    28861 cttgattttt cactgaatca aatgaaccca tctgtactgc accgttactg tagctatatc
    28921 cttcgcttga gtagccgaca tatcccatat tgattttgtc tgtgataccg ctatctgcta
    28981 aagtatcgac aaaacgatcg acaccaatct ttacttcacc gatccgatta ttgtcgttca
    29041 tacttcctga ccagtctacg accaatacga gatcaagagg cgtgatatct ttttgtacat
    29101 ttccacgagc attcaaataa acatcaaaca atccgggtgt acttgtttcc tttgcatact
    29161 ttcgtatcgc atagtctgct tgattaccgg ttcctccata ttcgatatac gaatgggtcc
    29221 gatcattagg atctccattc caagaattta taccatccca ttgatttgtt ccgtttttat
    29281 tgccttgatg atcaagaaca tttgtatttc ctgtaggttg ccaactggct ttaggataag
    29341 tgccctgatc atctgttgta tattctgtag gaatcgtttg attagatggc acactcacag
    29401 ctggcaagga taatgtaggc aatgtaaatg ggctgacaga attttcgttt tcttttgatg
    29461 attcttcttc tatttcgtct ggctttggta ctgacacatc tgtatctttc ttattttctg
    29521 tctcatcaat aatgtcatat tctttattat cgacgatcag cttgtttgcc tggctgtggt
    29581 aaagtgctgg atcgattgga aaactcagat cgatagtttg tttagtgttt gatgagattt
    29641 ccaacgtaat tttcccgcta tttacctgat aagcatagcc atctccttct tttctttcca
    29701 catcacttaa gtaattttcg actagaccaa tttctttttc tatcttttca gcactggtgt
    29761 tattcgcatt tagcttgatg ttcagtgctt tttttccaga ctgatccgaa atgacttcag
    29821 ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc tgctattgca gataatggca
    29881 caaagtttga taaactcaat attatcagca tgaaaataac tttcagtttc ttccctgtgg
    29941 tcgtcatttt tcccctcctt tttctttttt tctgttttga cgtgaatagt gaaaattatt
    30001 aaaataaaca ggcgttacat aattttattt attttaatat gataattatt atacgaaggg
    30061 cgctaaaggg aatcacacca attttcatac caattggttt catttatgaa gttgattcct
    30121 tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg agaaaaacat atgatcaggt
    30181 tgttggaacg aaactataga aagatggaaa aaatcattcg aattctgtcc atttctgaca
    30241 agtggatgac taaaaaagaa cttgccaaac gaattggtag ttcagaatct acatttgtaa
    30301 ggtatatcga agagatcaaa atccgctggg gaacggatgt aacgattcaa tcttcacaaa
    30361 aattggggta tcgtcttgaa catttgaatg taccagctta tttaaatgtt ctgatggaaa
    30421 tgtcgcaaag ttctacaaac agtcgtttat taaatgagat tattgtcaat ccgggtaaaa
    30481 cgattgagta ttattgtgaa aaaatttcca tcagccggtc ttcttttaca cgtaagttga
    30541 aacaatgcaa tcaaatttta gaaagctatt tattaaaagt gatcgttgac caaggttttc
    30601 agttaactag ccttaaaagt gagctccagt tgcgtatctt tgttaccttt ttctttttaa
    30661 gttactacgg aagacgcacg ttgccatttc caatcgaaaa aaagaagatc aaagaaatta
    30721 tggtcaagaa taattgtcag atgaatttgt ttcaggaaga aagcccatat gagcaatctt
    30781 tctttattat gtattttgtt gtgcatctca tgagagaatc ccaaggaact ccatttgaat
    30841 ggaaagctgg agactcttat gtacagttga taaatatcga taaaaaagac ttcttaatct
    30901 tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat aaaagtagtg aactattttg
    30961 tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa tgaaatcagt gagcagatac
    31021 gaaaaaatat gtcctactgt tcgttttcat ccgctgattg caaagaaaaa aacgaagaat
    31081 atgttgttca attattaacg caaatttatt ttttctcccg tattttccca tacgatacga
    31141 ctcatttgac gatccgtgca acttactttg cacagcagat gcaacaccgc cagatacgtc
    31201 tatatgctgg atttcgtgca gaacttcaat tttattccac tctactgaat cagaatctga
    31261 tttattattt atctagctat atcttttgga tgcttatcgg taatcctgaa attcaccatt
    31321 atacttctga taaaaaaata ctgattatca gcgacttaag tcttcgacat agtcaatata
    31381 ttgaagaata tatcagtgat attcttgctg tgcataaaat tcactctgag acaactgcta
    31441 ttactgaaga tcagctgtcg aaatacaatc ttctcgagta tgatctgatc gttacgaatc
    31501 aacccatttt gaatgcccat gttcctcata tattgattga tgatagtgtg tcattcgcaa
    31561 atgaagaaga actcatccgc ttattcgagt tatgaataat caaaaaacag ctatgtataa
    31621 accaccctga taaaaatttc aggacaaata cacatagctg tttcttttta ttgtattcct
    31681 tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt tgtccaatta
    31741 tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta taggaaacta
    31801 actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc cagccatttt
    31861 tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg atcatttcgc
    31921 tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc gcagcaccac
    31981 tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat gaattttaag
    32041 atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc atcttccaaa
    32101 tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac gatttctgag
    32161 actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc atcgattctc
    32221 tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat ctctccttta
    32281 tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct tctttttctc
    32341 ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt gaatcatacg
    32401 gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt taactagtaa
    32461 tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt ctgctaagat
    32521 caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt ctccaccaga
    32581 aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca gcaaggaatc
    32641 gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa attgttcctt
    32701 taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt ctttaaagcg
    32761 taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga tcactatttc
    32821 tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt tacctgaacc
    32881 agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa ttgctttcaa
    32941 agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca tcgttaatat
    33001 ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt
    33061 atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat
    33121 tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca
    33181 aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat
    33241 ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc
    33301 tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag
    33361 tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat
    33421 gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa
    33481 tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca
    33541 tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa
    33601 gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta
    33661 tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt
    33721 ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa
    33781 actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct
    33841 ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact
    33901 tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta
    33961 aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag
    34021 aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct
    34081 agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa
    34141 tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg
    34201 tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat
    34261 ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact
    34321 attagtagta gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat
    34381 cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt
    34441 ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc
    34501 aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt
    34561 ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta
    34621 tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga
    34681 tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta
    34741 taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca
    34801 aaataaggga atcattttga caaaagagca gatatttgac cggatctggg gatttgattc
    34861 tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag
    34921 tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt
    34981 gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg
    35041 tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc
    35101 aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag
    35161 tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg
    35221 aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca
    35281 ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc
    35341 gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata
    35401 ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag
    35461 cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca
    35521 agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc
    35581 tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt
    35641 ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga
    35701 ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat
    35761 ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt
    35821 tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata
    35881 cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa
    35941 aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc
    36001 atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga
    36061 tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc
    36121 cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat
    36181 ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg
    36241 aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag
    36301 tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa
    36361 atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga
    36421 aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta
    36481 cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc
    36541 tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac
    36601 ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt
    36661 aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt
    36721 gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac
    36781 cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt
    36841 ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt
    36901 cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt
    36961 catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc
    37021 tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg
    37081 tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa
    37141 ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt
    37201 ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc
    37261 ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa
    37321 tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc
    37381 ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat
    37441 gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta
    37501 tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg
    37561 gaatttgatt aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat
    37621 tctaataaaa tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca
    37681 ctcgttgtga tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag
    37741 gattaattct caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag
    37801 taccaactat ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat
    37861 tattgaataa atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata
    37921 aaagaaagag cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac
    37981 cacatagcta aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa
    38041 ataactaaaa atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg
    38101 cttcctaaga tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa
    38161 aatgaacact attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa
    38221 aagttaaagc aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat
    38281 cgttcttccc tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact
    38341 gattatttga gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa
    38401 aaacaagatt atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact
    38461 acagcgaacc aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag
    38521 caatattcat taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag
    38581 atttctcttt tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat
    38641 gcagaacttt tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc
    38701 agagaagcta gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt
    38761 ctgtattgcg gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg
    38821 accaagcaga ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca
    38881 aaagaagcga tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa
    38941 aattcttttg tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa
    39001 attttgagca aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat
    39061 cgttattatg cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat
    39121 cgatacctct catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat
    39181 cacgaattag cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt
    39241 tcacaataaa ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat
    39301 tacttaacaa aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac
    39361 ttatttacga attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat
    39421 gagccacttt cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa
    39481 agtgtgttga aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt
    39541 attatttata agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt
    39601 ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc
    39661 gatcgtttag aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa
    39721 atttcaaatg gaacaaaatc aaaacagatt c
//
LOCUS       DACCDF010000018        38334 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCDF010000018 DACCDF010000000
VERSION     DACCDF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.018"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            161..391
                     /locus_tag="IVK51_000915"
     CDS             161..391
                     /locus_tag="IVK51_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP6430527.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IVK51_000916"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IVK51_000916"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6430528.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IVK51_000917"
     CDS             1802..1978
                     /locus_tag="IVK51_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6430529.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IVK51_000918"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IVK51_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP6430530.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IVK51_000919"
     CDS             2593..3060
                     /locus_tag="IVK51_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430531.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IVK51_000920"
     CDS             3145..4029
                     /locus_tag="IVK51_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6430532.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IVK51_000921"
     CDS             4146..4379
                     /locus_tag="IVK51_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP6430533.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IVK51_000922"
     tRNA            4467..4547
                     /locus_tag="IVK51_000922"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IVK51_000923"
     tRNA            4554..4625
                     /locus_tag="IVK51_000923"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IVK51_000924"
     CDS             5140..5847
                     /locus_tag="IVK51_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6430534.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IVK51_000925"
     CDS             complement(5907..6551)
                     /locus_tag="IVK51_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP6430535.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IVK51_000926"
     CDS             6678..6965
                     /locus_tag="IVK51_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430536.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IVK51_000927"
     CDS             7073..7393
                     /locus_tag="IVK51_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6430537.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IVK51_000928"
     CDS             7474..8787
                     /locus_tag="IVK51_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6430538.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IVK51_000929"
     CDS             8784..9503
                     /locus_tag="IVK51_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP6430539.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IVK51_000930"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IVK51_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP6430540.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IVK51_000931"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IVK51_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP6430541.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IVK51_000932"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IVK51_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP6430542.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IVK51_000933"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IVK51_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP6430543.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IVK51_000934"
     CDS             14940..15833
                     /locus_tag="IVK51_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430544.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IVK51_000935"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IVK51_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6430545.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGGKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IVK51_000936"
     CDS             17770..18627
                     /locus_tag="IVK51_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP6430546.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IVK51_000937"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IVK51_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP6430547.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IVK51_000938"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IVK51_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP6430548.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IVK51_000939"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IVK51_000939"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6430549.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IVK51_000940"
     CDS             21383..22141
                     /locus_tag="IVK51_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP6430550.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IVK51_000941"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IVK51_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP6430551.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IVK51_000942"
     CDS             23069..24100
                     /locus_tag="IVK51_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6430552.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSFLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IVK51_000943"
     CDS             24097..24864
                     /locus_tag="IVK51_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP6430553.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IVK51_000944"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IVK51_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP6430554.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IVK51_000945"
     CDS             26832..27149
                     /locus_tag="IVK51_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP6430555.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IVK51_000946"
     CDS             27149..27499
                     /locus_tag="IVK51_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP6430556.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IVK51_000947"
     CDS             27666..28604
                     /locus_tag="IVK51_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP6430557.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IVK51_000948"
     CDS             28619..29449
                     /locus_tag="IVK51_000948"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP6430558.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IVK51_000949"
     CDS             29493..30518
                     /locus_tag="IVK51_000949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6430559.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHHASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IVK51_000950"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IVK51_000950"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6430560.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IVK51_000951"
     CDS             31596..32036
                     /locus_tag="IVK51_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP6430561.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IVK51_000952"
     CDS             32039..32641
                     /locus_tag="IVK51_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6430562.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(32704..33807)
                     /locus_tag="IVK51_000953"
     CDS             complement(32704..33807)
                     /locus_tag="IVK51_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP6430563.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            34017..35018
                     /gene="ccpA"
                     /locus_tag="IVK51_000954"
     CDS             34017..35018
                     /gene="ccpA"
                     /locus_tag="IVK51_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP6430564.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            35373..35768
                     /locus_tag="IVK51_000955"
     CDS             35373..35768
                     /locus_tag="IVK51_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430565.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            35768..36019
                     /locus_tag="IVK51_000956"
     CDS             35768..36019
                     /locus_tag="IVK51_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430566.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            36062..36457
                     /locus_tag="IVK51_000957"
     CDS             36062..36457
                     /locus_tag="IVK51_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430567.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            36454..37710
                     /locus_tag="IVK51_000958"
     CDS             36454..37710
                     /locus_tag="IVK51_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP6430568.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGQLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            37722..38045
                     /locus_tag="IVK51_000959"
     CDS             37722..38045
                     /locus_tag="IVK51_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430569.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            38038..38304
                     /locus_tag="IVK51_000960"
     CDS             38038..38304
                     /locus_tag="IVK51_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430570.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        12859 a         6514 c         8261 g        10700 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtc
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 gggggaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gtttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgt gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcatgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 gatcgatgac tatgagcgta cacacgcttc tacgattgcc gttatgcaag ttcctcacga
    31021 tgaaacttca aaatacggaa tcatcaaccc aggggaagta ctggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tgggcgttac ttattgactc cagaaatctt tgatgtatta tcagaacaac aaccaggtgc
    31201 tggaaatgag atccaactaa ctgacgcaat cgatacgttg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttatga cgttggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctga cacatccaga agtaggggca cctttgcgtg aatacatcat
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc aatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata atcttttttt ctaacgagct tcagcggcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgttttcat cgttcgcgta ttgttgcaag
    31681 tttccaaaac tttagaaaaa gtagaccaga ctgtcgcaga agcaaatacg acaattgaga
    31741 tcgtcacaca agacgtagat cttttatcta gacaagtaga aggcttgcta gtcaaaagta
    31801 atgagctgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcggaac ttaatcattc tagtaaaaac atcgtgacga
    31921 aagtcggcgg aatcggaaaa acgacagcta aagcgactgt tgccggaaaa gtcggtggaa
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataagaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagttgga ggtacagccg cagcaatcgc
    32101 cgcattattg ttagcaccca aatctggtaa agaacttcgg gatgaattgg ctgctcaagc
    32161 agatgatttg atggacatgg cttcagatta cacagatatg gcaaaagaaa aatcatctga
    32221 gttaacaggt attgcaaaag aaaaagcgca aggtctttct caacaagcag gggatctagc
    32281 aggcaatgtt aagcagaagg taagtgaatc agcagaggaa gcaggaagtg aatcagatga
    32341 tatcttgtta aacctcaaag aacaatcagc tgatttatct aatcgtttca aaaaaacagc
    32401 agaagatctg aaaaagccag cagacgaatt aggtagtatt gctaaagata cttcagaaga
    32461 tatcatgatc gatgtaaaag atgctgcaga taaagcagga gagacggtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgacac aaaagaaatg ttgaaaatgg caaaagaaga
    32581 tgcaaaagaa gctaaaaaag aaatgaaaga tacaatgaca gattcgacta ccaatcacta
    32641 agattactca tttctagaat aaaaaaggtg agagcggacc agctctcacc ttttttattt
    32701 tcattactct aaaacaagta attcttttgg tgtagtcgtg aatggcacac aaccatcact
    32761 tgtcacatac acacaatctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32821 ttcaagagag aagcacatcc cttcttcaat cactaagtcg tttccttcta caagagaagg
    32881 gaattcgtgg acggttgttc cgattccgtg acctaaacgg tgattgaaat attcgccgta
    32941 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    33001 gcgaactgcc tcttgtgcgg caagctgtgc ttcaagagtg atgttataga ttttttcttg
    33061 gaactcagtt ggtttttgat aagcaactgt acgagtagca tcactgcaat agccattcca
    33121 aataacgcca agatcgaata acactagttc atttggtgaa accttcgtgc ttcctggtgt
    33181 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33241 aacaccttgt ttttttaatt ggtattcgat ttctgcgata attgcttgtt cagttacgcc
    33301 ttcttttact gttttgaagc caatttcaaa ggcaacatcg gcccatttac cagcttcgat
    33361 cagtttgtct ttttcttcta ccgttttgat cagttgcaga cgctggataa tagaagttag
    33421 atcggtgtaa gaatctgtac ctgtcaagat atcagagata gcatcgaagc gatcgacagt
    33481 cagagcattc ttttcaatac ctacattacc aggttgccca tagcgtgcgc gaagctcttt
    33541 tccgatgatc tcccaaggat tttcgctgtc taagtagcca cgtacatcgt acgcccagct
    33601 gctgttttga gcatcttcaa cttccagagc aggagtgaaa aggaaaggat cttgttccag
    33661 agggataaaa agagctagta cacgctcgtg aggatcactt tcatatccgg aaaagtaagc
    33721 gatgtggcca gggtcgctga tataagccaa ttctacttgg tgttgtgcca tccattgacg
    33781 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33841 caataattgt accactgatt gattaaaaga ccaacgttct taaaactgaa aaaagtaaaa
    33901 attttcatga aaacagagat aaaacggttg caattgttga gattatttga tatcctattt
    33961 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    34021 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    34081 tttcacgtgt cgtaaatggc aatccaaacg tcaaaccagc aacaagaaaa aaagtcttgg
    34141 aagttatcga tcgcttggat tatcgtccaa atgcagtcgc acgtggcttg gcaagtaaga
    34201 aaacaacaac agttggggta atcattcctg atgtcagcaa tatgttcttt gcctctttag
    34261 cacgtggtat cgacgatgtg gcaacaatgt acaaatacaa tatcatctta gcaaactcag
    34321 atggcaacga tcaaaaagaa gtcaatgtat tgaataattt attggctaag caagtggacg
    34381 gcgtgatctt tatgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34441 aaacgcctgt tgtactagct ggttccattg atccagatga acaagtaggt agcgtaaaca
    34501 ttgattatgt gtcagcaaca aaagatgcag taaataaatt agcaaaaagc ggcaataaaa
    34561 aaatcgcttt tgtcagcggt gcgctgattg atcctatcaa tggtcaaaac cgtctaaaag
    34621 gctataaaga agcattaagc gaaaacaatc taccatatag cgaaggattg atttttgagg
    34681 cacaatacaa ctttaaagat ggtctgtctc tagttgaccg gatccataat agcggtgcaa
    34741 cagctgtcta tgtttcagac gatgaattag ccattggtat cttggatggt ttattagacc
    34801 gcggcgtaaa agtaccagaa gaattcgaaa tcatcacaag caacaattct ttactgacag
    34861 aagtagctcg tcctcgtttg acaagtatca cacaaccatt atacgattta ggagcggtat
    34921 ccatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34981 tgccttatgg aatcgaagaa aaaggatcaa ctaaataata aaagatcaat catttaaagg
    35041 ttgttccaaa agtggtcaac ttctggaaca acgtttatac gagaaagggg gttatgacaa
    35101 aattttgtca taaccccttt ttttttgtga ctttaaaaaa agagaaaatg gcggaaacaa
    35161 accttacgtc catcgtatac gacacaatct gtgcgtagat gctacatttt gttcactact
    35221 atcaaaatta ctttaaaatg aaaacgtgat aaaaaagaaa gtagaaaagt agataagtaa
    35281 ctctaaaaaa tccaaaaaaa tccaggctcg attgcgtact tatcttaaag gagaaacaat
    35341 ccatcatata taacccgaaa ggagacttct atttgcctaa gaaaagacga aagaccatag
    35401 tcatcatttt gaccattctt actataggaa tcggagggaa attttacatg gacaaaagag
    35461 aaatgcaaag acagaaagat ttactcgcag tagaaaaaca gtccgtcaaa gttctgaaaa
    35521 atacctttgc ggatatcaag gaagtgaaga ttgaaaaatc tgctcgaaat gaaatgacgg
    35581 gttcataccg gatagttatt ctaatgacta ataaacaaga tcaatcgatt tatttttctt
    35641 atagtttttg gaaagaaaga aatgagatag gaagttacgg aatagttgat gaaaaaaagc
    35701 aaaaagaagg aaatacgctc aataaagtta aagtaactta ctctaatgga aatgaggaaa
    35761 gtatttaatg attactgaca aacaatatag tgaattaagt gatgctgttt actggctaga
    35821 tcctaaacat agagactatg tacctgaaat gcaagaaaat ttaagtttta aaattaacgg
    35881 cactatgtat aaaatcctaa aaatcaaaaa ctccttcgac ggcatgcagg cgatggctgt
    35941 agcgccaatc gtccatagca aattggagaa aaatttcaaa aataaaaaaa ttcctgcaaa
    36001 ctttcgcgta cttaaataat gaaggtgtca atacttacat aaaaagaaag gaggcacgtt
    36061 cttgtcaagg aaaataagaa aactcagctt agccatttta ttaatcgtca tattagggat
    36121 cggagggaaa gtctatatgg acaaaagaga agcacaaagg cagaaagatt tactcgcagt
    36181 agaaaaacag tcagtcaaag ttctgaaaaa tacctttgcg gatatcaggg aagtgaaggt
    36241 agaagaattt aaaaaaaacc ctgttaccgg aagttatggt gcacttgtga caatgacaaa
    36301 taatgaaggg aaatctgttt attttgatta tagtttttgg aaggagagaa atgaacttgg
    36361 tggttttggc attgagaaca gagcggtaca aaagacagga gtcactataa aaagagtaaa
    36421 agtaattttt tctaacggac aggaggagtt agtatgacaa taaacgataa gcattacaat
    36481 gatattagtg agaaggttta ttggctagat cccaaatatc cccgctacaa tgagggatac
    36541 aaaaagaact ctgttaaaga atttgctggt atggaatttc aaatcctaca aatcaaagac
    36601 tcattggacg gcatgcaggc gatgtctgta gcgccaatca agaacggtaa agtggatctt
    36661 tctcaagtag tgattgcttt tgcaggaacg aacttaagtg attggaaaga tttggaaacg
    36721 gatgcaagaa gtatcatgaa tctctacggg aatagaccag gagcaagttt ttctgaaaaa
    36781 gcaacaacaa acaagatttt atccgttagt ggattgaaaa cagcggggca aatcaattca
    36841 gcgattgcat ttgctaatga agtaaaaaag gaatatggag aagctgctat ttctactacc
    36901 ggccattcat taggtggatt cttagcatta tatgtagcag cagagaaaca atggaaaaat
    36961 gtcggcttca acgggccgga cccgtatgat ctcttatcac cagaagcaaa aaaatgggtg
    37021 gaagaaaatc ccggtatgct caccaattac cgaaatcacg ccgattttat cggtaatttt
    37081 ggtggtaatg gcacaggtgc tgaagttcgg atcagtatgg aaacagcatt cttgaatctg
    37141 cttgcttccc acagtttaac ctcttggaag tttgatgaaa atggaaagct gattattcct
    37201 gaaaatgaaa acaatgttaa agctcgaaaa caacagaaag aaaaccaagc gacccagttg
    37261 ttttcctatc agttgcaaga actgaacaac atacgtaaaa aactacgatt aagtggcggc
    37321 cagctgacag ttaatgaaca aatttactta gatgacacac aagctaggtt agtagtggaa
    37381 aagttatcgg caaaaatgaa aagcgctatg gaagaagtta tccgcatcaa tcaaagagga
    37441 atttttgagc tagagaaaaa atggcaagaa ggcttgcggc aagcacgtgt tgtagcccct
    37501 tcgttgactg aacaggaaat catagaagca ttagctagtg tgggcgccac aaaagaaaac
    37561 ttagtcgatt ctgcgaaaga agtctatctg ctacgaatca ataaggcaag acgtatgggg
    37621 gaggcctttg atacattggc gaaggagatc caagacaaaa tcaatgaaat cgtggcacga
    37681 gatcgagaat tagcacagca attcaattaa aggagaagta gatgaaacta gaagaattgg
    37741 aaagagatta tcgaagtgaa cgaagacaat tagaagctca agaagatgaa ttgatccact
    37801 ttaagaatca aggattacga ttgatggagc aagaggagca gcgactgcat catgtattaa
    37861 agaacttagc tttagataat gagcctctgt ttcaagcaag acgaagttat tcgaattatc
    37921 aggagcatta tcaacaagcc ctacaagtag aacgacgaaa actctatcag cagcaagatg
    37981 aactagaacg ttcctatcgt caaaaaatca gacaaataac tatggagaaa tcatcttatg
    38041 aatgaatttt taatgcaatt actaaaagaa ttagagaaaa atctacatcg atcaaatgta
    38101 cgttcagttg gtatggggat atctcagcaa atttgggcag aaaaacgatt gacagatcaa
    38161 aactaccaaa tgactgcaga agaagctgct caagaatttt ctaaccgaca tgtacacttg
    38221 aaaggatcag taaaagggca atttgcagaa aaactaactg aattattcca aagacaagaa
    38281 aagaaattaa aacagtttcg ttagtaaagt tatatagaaa aattttgtga aaat
//
LOCUS       DACCDF010000019        38176 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCDF010000019 DACCDF010000000
VERSION     DACCDF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.019"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            308..1192
                     /locus_tag="IVK51_000961"
     CDS             308..1192
                     /locus_tag="IVK51_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430571.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            1401..1979
                     /gene="pgsA"
                     /locus_tag="IVK51_000962"
     CDS             1401..1979
                     /gene="pgsA"
                     /locus_tag="IVK51_000962"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP6430572.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2219..3460
                     /locus_tag="IVK51_000963"
     CDS             2219..3460
                     /locus_tag="IVK51_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP6430573.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3569..4618
                     /gene="recA"
                     /locus_tag="IVK51_000964"
     CDS             3569..4618
                     /gene="recA"
                     /locus_tag="IVK51_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP6430574.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5029..6585
                     /gene="rny"
                     /locus_tag="IVK51_000965"
     CDS             5029..6585
                     /gene="rny"
                     /locus_tag="IVK51_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP6430575.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IVK51_000966"
     CDS             complement(6664..7470)
                     /gene="proC"
                     /locus_tag="IVK51_000966"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP6430576.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7611..10184
                     /gene="mprF"
                     /locus_tag="IVK51_000967"
     CDS             7611..10184
                     /gene="mprF"
                     /locus_tag="IVK51_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6430577.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10338..11189
                     /locus_tag="IVK51_000968"
     CDS             10338..11189
                     /locus_tag="IVK51_000968"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP6430578.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            11434..12372
                     /locus_tag="IVK51_000969"
     CDS             11434..12372
                     /locus_tag="IVK51_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP6430579.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12395..13075
                     /locus_tag="IVK51_000970"
     CDS             12395..13075
                     /locus_tag="IVK51_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6430580.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13048..13872
                     /locus_tag="IVK51_000971"
     CDS             13048..13872
                     /locus_tag="IVK51_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6430581.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14039..14857
                     /gene="purR"
                     /locus_tag="IVK51_000972"
     CDS             14039..14857
                     /gene="purR"
                     /locus_tag="IVK51_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP6430582.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15360..16733
                     /gene="glmU"
                     /locus_tag="IVK51_000973"
     CDS             15360..16733
                     /gene="glmU"
                     /locus_tag="IVK51_000973"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP6430583.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16827..17798
                     /locus_tag="IVK51_000974"
     CDS             16827..17798
                     /locus_tag="IVK51_000974"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP6430584.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17804..17906
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17972..18541
                     /gene="thiT"
                     /locus_tag="IVK51_000975"
     CDS             17972..18541
                     /gene="thiT"
                     /locus_tag="IVK51_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP6430585.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNSWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18622..19395)
                     /locus_tag="IVK51_000976"
     CDS             complement(18622..19395)
                     /locus_tag="IVK51_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430586.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19661..21361
                     /locus_tag="IVK51_000977"
     CDS             19661..21361
                     /locus_tag="IVK51_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP6430587.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21331..21549
                     /locus_tag="IVK51_000978"
                     /pseudo
     CDS             21331..21549
                     /locus_tag="IVK51_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21569..22459
                     /locus_tag="IVK51_000979"
     CDS             21569..22459
                     /locus_tag="IVK51_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP6430588.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22477..22761
                     /locus_tag="IVK51_000980"
     CDS             22477..22761
                     /locus_tag="IVK51_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430589.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23248..23862
                     /gene="gmk"
                     /locus_tag="IVK51_000981"
     CDS             23248..23862
                     /gene="gmk"
                     /locus_tag="IVK51_000981"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP6430590.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23867..24172
                     /locus_tag="IVK51_000982"
     CDS             23867..24172
                     /locus_tag="IVK51_000982"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP6430591.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24425..26833
                     /gene="priA"
                     /locus_tag="IVK51_000983"
     CDS             24425..26833
                     /gene="priA"
                     /locus_tag="IVK51_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP6430592.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26848..27339
                     /gene="def"
                     /locus_tag="IVK51_000984"
     CDS             26848..27339
                     /gene="def"
                     /locus_tag="IVK51_000984"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6430593.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27332..28270
                     /locus_tag="IVK51_000985"
     CDS             27332..28270
                     /locus_tag="IVK51_000985"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP6430594.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28270..29628
                     /gene="rsmB"
                     /locus_tag="IVK51_000986"
     CDS             28270..29628
                     /gene="rsmB"
                     /locus_tag="IVK51_000986"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP6430595.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29641..30381
                     /locus_tag="IVK51_000987"
     CDS             29641..30381
                     /locus_tag="IVK51_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP6430596.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30378..32447
                     /gene="pknB"
                     /locus_tag="IVK51_000988"
     CDS             30378..32447
                     /gene="pknB"
                     /locus_tag="IVK51_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP6430597.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32612..33511
                     /gene="rsgA"
                     /locus_tag="IVK51_000989"
     CDS             32612..33511
                     /gene="rsgA"
                     /locus_tag="IVK51_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP6430598.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33524..34174
                     /locus_tag="IVK51_000990"
     CDS             33524..34174
                     /locus_tag="IVK51_000990"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6430599.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34175..34813
                     /locus_tag="IVK51_000991"
     CDS             34175..34813
                     /locus_tag="IVK51_000991"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP6430600.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            34968..35822
                     /gene="mreC"
                     /locus_tag="IVK51_000992"
     CDS             34968..35822
                     /gene="mreC"
                     /locus_tag="IVK51_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP6430601.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35826..36335
                     /gene="mreD"
                     /locus_tag="IVK51_000993"
     CDS             35826..36335
                     /gene="mreD"
                     /locus_tag="IVK51_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP6430602.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36590..38128
                     /locus_tag="IVK51_000994"
     CDS             36590..38128
                     /locus_tag="IVK51_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430603.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTVPESSATEE
                     SKTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVTPAPAP
                     SGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWTVPQES
                     AGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQWFAPDF
                     AVSM"
BASE COUNT        12554 a         6828 c         8304 g        10490 t
ORIGIN      
        1 acggaaaaat atctccttat attttgccgg tttttgagct aataccgagg aacagctgat
       61 tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttta actccgagtc acaaggaaca
      121 agttcaaaaa acagcttctt atatttattg gatattgtga cttgtgaccg aggagttggc
      181 aggagaacaa tgtttattcg gaaaaaagga gctgtgacat gacttttgtc acagcaccca
      241 aacataacgt ctttcgttgc aacagcagat gcttcggaga atcaaacaga ctggagaaga
      301 tgaacgagtg gcttttgaaa ccatcggaga aaaattacgg caagcacgcc tgaataaaaa
      361 aatatcttta gatgaattac agcagattac aaaaattcaa aaacgttatt tggaagcaat
      421 cgacaatgat gattttgacc aattgcctgg taaattttac gtgcgggcat ttattcggca
      481 atacgctgaa gctgtaggag aagatggaga ccatttagtc gatgtttttg atgggaaaag
      541 caaattgaca ggatcggtcc ctgaacgtcc gcagcctgaa acagtaagta cttctagaaa
      601 agcaatgcat caggaagaat cgcatccaag tcgcttttgg acaagtcttc ctgtcatttt
      661 attaggattg gttgctttgg cgattgtcgt tgtcgtgggc tatatgactt ggcaagatcg
      721 tcaagctgat ccaataatcg gagacacagc ttcttcgctt aaagtagaag gatcagtcga
      781 ggagtcctct tcctcagagc aaagcacgca gaccaaagag tcttctacag tagaatcaac
      841 aacaaaatca agtgaagaaa agaaaatggc aatagctaca ggagaagatt ctggcagtgc
      901 catcgcagta aatatcacag acgctaccaa gcctgtaacc cttgaattta ctgctgtcaa
      961 tcgtgtatgg ttaggtgtgc tagtagacaa tgcctatgtt tatcaaggga cactggcagc
     1021 aaatgaaaca cagagtaccg tgttaccgga aacagcaaca aatgcgacca tcacgattgg
     1081 tgcagcaagc aatgcaacga tcaaagcgaa tggagaatcc gtaccagtta atccagggga
     1141 aaacaatcag agtccaaaaa atgtgaacct tactcttcaa tacgcagaat gatcgtataa
     1201 attagtttac gcatctgttt acttgtaggt ataaaaaaga aggatcgtga caaatctgtg
     1261 tcgcggtcct tcttcgttaa ttcaaaaaga tcaatttcaa taaaaatact cctttgaata
     1321 aattcatttg gaaaagtaaa taatatcggt ggaaacttcg ctgatagtca cgtagaatag
     1381 ggaagaaagg agtcgatagt gtgaatttac caaataaatt aactgtactt agaatttgta
     1441 tgatcccgat tttcattttg gttgtaggtt tacctttaga ttggggacaa tttaacgtag
     1501 gagacaccac actggctgtc acacagctag taggtgcagt tatttttgct ttagctagta
     1561 tcacagactg gttagatgga aaaattgccc gtgcgcgagg ccttgtcact aattttggca
     1621 agtttgcgga cccgttggca gataaaatgc tagttatgac tgcttttatc atgttggttg
     1681 accaaggaaa ggcccctggt tgggtagtag cgatcattgt ctgtcgtgaa ttagccgtca
     1741 caggcttgcg tttactactt gtcgaacatg gagaagtcat ggctgcagct tggccgggaa
     1801 aagtcaagac ggctacacaa atggttgcta ttatcttgct gtttatcaat aatatcccat
     1861 tcagcctgat cggtattcca gtagatcaaa tcatgcttta tgcttgtctg atctttacta
     1921 tctattcagg tgtggattac ttcatcaaaa acagcggtgt gttcaaagga tcaatgtaag
     1981 tatcgtttag tggagaggtt gtgagcctgc cgtctaactc tgagtcacaa gggataattt
     2041 caaaaaacag cttttcatgt tttatgtata ttgtgactta tgaccgaggt tttggcagga
     2101 gaacactgtt tatttggaac aaggagctgt gacaagattt ttgtcacagc tccttgccct
     2161 ctttttaaat aaaaaaatat aaaaatacag cgtatactat ataagggagc gggaagtaat
     2221 gaaagcagaa ataatagctg tcggaacaga acttttacta ggacaagtcg tgaatacgaa
     2281 tgccactttc ttgtcagaac agctagctga tctaggtatc gaagtttatt atcaaacagt
     2341 tgtaggagat aatcagcagc gtttagaaga gttgattgct ctagctgaaa caagaagtga
     2401 actcatactc ttatgtggcg gattaggacc gacagaagat gacctgacaa aagaagcaac
     2461 tgcggctcac ctcggtaagt ctctcataca aaatacggaa ggatacaaaa aattattggc
     2521 atattttgaa acaacccatc gaaaaatgac caaaaacaat ctgcaacagt cccagattat
     2581 cgaaggcggg gttcctctac caaatcgtac agggcttgct ttagggactt tttatcaaac
     2641 agatacacat gcgtatatcc ttcttcccgg tccgcctaat gagttgaaac caatgtttgt
     2701 ggaacaagta cgtcctttac tagaggaacg ttttccttct gaagaaaaac taatttctaa
     2761 agttttgcgc ttttatggga ttggtgaatc gcgactggtc actgagctaa aagatctgat
     2821 tgagacacag atcaatccaa cgatcgcacc atatgccaaa ccaaatgaag tgacattacg
     2881 actgacagtg aaaacaaatg atgttcaggc agggaatcaa gctcttttag cattagaaga
     2941 aaaaatccaa gaacgagtag gggaatattt ttatggatac ggcgatgata attcgctagc
     3001 aaaagtcgta gtggaactac tgaaagaaaa caagcagact gtaacagcag cggaaagttt
     3061 gacagccggc gccttccaag ccgcattagg cgacattgct ggtgtatctg aagtatttcc
     3121 aggcggattc gttacctact cgctccaaac aaaagctggt tttctggaga ttgatccgga
     3181 gctattagca gaatatggaa cagtcagcaa agaatgtgtg gagcagatgg ccattcaagc
     3241 acgtgagata gcaagaacag attacagtgt agctttcacc ggagtggctg gaccggacac
     3301 gttagaaggt caatctgcag gaacagtatg gatcgctatt tcatctaaag acggcgtggt
     3361 ttcggaaaaa tatcactttg ccagagatcg tgctgctatc agatataatg cggttatgca
     3421 aggatttgat cttttgagaa aacagctttt aagaaaatga taaatgcgaa tatttgttcg
     3481 gttttttctt gctttttttc actaaaaaag atatgataag gataatgaaa tgttgctctg
     3541 atttgtaaat ataaggagga ttatcgtatt ggcagatgat cgtaaagcag ccttagatgc
     3601 tgcactgaaa aaaattgaaa agagctatgg taaaggctct attatgaaat tgggcgaaaa
     3661 aatcgaccaa caaatctcta caattccaag tggctctctt gcgttagatg tcgctttagg
     3721 tgtcggcggt tacccccgtg gacgtatcat cgaagtatat ggacctgaaa gttcaggtaa
     3781 aacaacagtt gcactacacg ctattgcaga agtacaaaaa aatggcggaa cggccgcttt
     3841 cattgatgct gagcatgcgt tagatccgca atatgcacaa aaattaggtg tgaatatcga
     3901 tgaactactt ctttcacagc ctgacacagg agaacaaggt ctagagatcg ctgatgcttt
     3961 agtatcaagt ggggctgtag atatcgtagt agtcgattca gttgctgctt tagttccacg
     4021 agcagaaatc gacggcgaaa tgggtgactc acatgtcggg ttacaagcac gtttgatgtc
     4081 tcaagcattg cgtaaactct ctggttcgat caacaaaaca aaaacaatcg ctattttcat
     4141 caaccaaatc cgtgaaaaag taggcgtgat gtttgggaat ccagaaatca ctcctggagg
     4201 acgcgcattg aaattctacg caacgatccg tttggaagta cgtcgtgcag aacaattgaa
     4261 acaaggtaca gatattgttg gtaaccgtac aaagatcaaa gtcgtaaaaa acaaagtagc
     4321 acctcctttc aaaatcgctg aagtagatgt catgtatggt ttagggatct cacaagaagg
     4381 agaacttcta gacatggcag tagaaaaaga cattgttgat aaaagcggtg cttggtattc
     4441 ttataaagaa gatcggattg gtcaaggacg ggaaaacgct aaaatctata tggctaatca
     4501 tcctgaaatg atggcagaag tttctgctct agttagagca gcttatggta ttggtgaaga
     4561 agtcgctgtc ccagaagatg aaaaaggaca agaagaattg cctttagtag aagaataaaa
     4621 attatgataa acaaaaacgg ctggtctgaa gagtaggtac tctccttgac ctgccgtttt
     4681 tttattgaat aaagaaatcg tgagttattt aattatacga gtgggtacat aaaataaaaa
     4741 tatctgttca tctacaaaga ataatttaat agaaaagttt ctcaatttaa aggtttacct
     4801 atatgaaact agtgtcattt attttaaaaa ccatcgttta atgaataagt agaaagccgt
     4861 ttctttgttt atttaaagtt gacactacgg ggtacaaaca ttagaataaa gttgtgcatg
     4921 ttttttgtac acatatatgc gaagatgcat attgacgagt ttagaataat ttatcataga
     4981 agctgtatag atgataaaaa tgaaaaagat agtatggagg tggattttat ggtattcaac
     5041 attctcctcg ctatcatcgg tttaattgtc ggtcttggtt tagggtttat ggtcgcaaag
     5101 tcacgtcacg agaaagaaat agctggtgca caatcatcag ctgctggaat catcgatagt
     5161 gcaaaaaaag aagcagaaac tctaaaaaaa gaagcattgt tagaagctaa ggaagaaaat
     5221 cagaaatacc gttctgaagt tgaaagtgag ctgagagaaa gtaagttaga attaaaatct
     5281 caagagaatc gtttgattca acgcgaacaa acgctgaatc gtaaagatga ctcgttagaa
     5341 aaacgcgaga actcacttga aagtaaagaa gaagcactta gttcaaaaca gcaattaatt
     5401 gaagagcgag aaaaagacgt tgaaaaaatg attgaagatc aacaaaaaga actagaaaaa
     5461 attgctggtt tatcaaaaga ggacgcacga gatgtcatca tgaaatcgac tgaagaagag
     5521 ctgaatcatg agttaactct aatggtaaaa gaatctgaac agcgtgccaa agaagaagca
     5581 gatcgcaaag cgaaaaactt attatcatta gcgattcaac gctgcgcagc agatcaagtt
     5641 tcagaaacga ctgtttcagt cgttaccctt ccgaatgatg aaatgaaagg tcgtatcatt
     5701 ggacgagaag gtcgtaatat tcgaacgctt gagacgctga ctggtatcga tttaatcatc
     5761 gatgatacac cggaagcagt tgttctttcc ggattcgatc caatccgaag agaaatcgca
     5821 cggatgacct tggaaaaact gatacaagat ggacgaatcc atccagcgcg tatcgaagaa
     5881 atggtcgaga aatctcgtaa agaaatggat gaacgtattc gcgaatacgg ggaagaggca
     5941 gcgtttgaag tcggtgctca tacacttcat ccagatctaa tcaagatcat cggacgtctg
     6001 cgattcagaa ctagttatgg acaaaacgtc ttgaagcatt cggtcgaggt tgcaaaatta
     6061 gctggtatct tagcagcaga gcttggcgaa gatatccaat tagcaaaacg tgccgggttg
     6121 ctccatgata ttgggaaagc acttgaccat gagattgaag gatctcacgt tgagatcggt
     6181 gcggaattag ctgccaagta caaagaaaac ccagtagtaa tcaacgcgat cgcttctcat
     6241 catggagatg tggaagctac ttctgttatc tctgtccttg tagctgcagc agatgcgctc
     6301 tcagcagcaa gacctggtgc gcgaagtgaa tcattagaaa actacatccg tcgtttaaga
     6361 agtctggaaa atatttcgaa cagcttcgcc ggtgtagaat caagttacgc agttcaagct
     6421 ggacgcgaag ttcgggtaat ggtcaagcca gaagaaatct cggatttaga cgctgttcgt
     6481 ttagtacgtg atattcgtaa acgaatcgaa gaagaactgg attatcctgg acacatcaaa
     6541 gtaatcgtta ttcgggaaac tcgtgcaact gattatgcta aataataaaa aatacaaaag
     6601 aagagccgcg gcaaaagcca cagctcttct ttttttgtaa ggtacgaaaa aacattgcta
     6661 tcattatttt ttattcaaca tctcaagatc tttctcgatc gtcgtattga ttcctttgat
     6721 gattgctggg atgaatcctg cttcttctaa ggcaactaca cctgcaacag ttgtaccgcc
     6781 aggagaagaa acttggtcta caagttccca tggattttta tcacttgaaa ggaatttttg
     6841 acaagaaccc ataattgctt ttgcagcaat ctcagtggct gtctgtttgg acaaaccatt
     6901 cagtacacca gtccgtccca tcgcatcgat cagcatgtaa acgaatgcgg gtgagctgcc
     6961 agcaaggcca atgaatgtac tgaaatctga ttctgctaaa ggatagacgc tgccgattgc
     7021 ttcaaaaatc atcgtagctt gctgaagctg ttcctccgtg gcagcttcat ttttgcaaat
     7081 cgcagaaacg cctgctccta cgctgacatt gacattcggc atcacacgta cgatggaggc
     7141 ctgaggatct tcaagcgctg tttctaaatc tgttaatgat tttcctgaag caatagaaat
     7201 gaataagact ttctggctat attctcttag ttcttccaat actttttcaa tgatttttgg
     7261 tttcacggct aaaagaacga tatctgattg tgatgcaact tcctgattac ttttgcatgc
     7321 atggacgcca gtttcagcag caaatttttg tgcattttca aaatgtccac tggaaacgaa
     7381 cacctcttct ttcgagactg ttcctttttt taataaacca tcaataatag cttgtgccat
     7441 attccctgtg ccgataaatc ctactttcat gttatcctct cctttagcat taggatatct
     7501 ttctttttct atcatcgcaa gcaaagaaca ggtctaaagt ggaaaaatta aaataaataa
     7561 gataacattg gggtatgaca caaaagtata caagttagga atgggaaaat gtgaaaaata
     7621 aacgatactt gatgctgcaa tggataaagg aacatagtct gattttaaaa ctgatttttt
     7681 tcggctccgt gttagtcttt gtggcaaatc aagtcgcaaa tattgctaat ggcatgagtt
     7741 ggcaagatat ttggcaaagg atgggacaac aaagccgcat gacgatcctg ctcatgattc
     7801 tggctggact gctgggtgtc actccgatgt tgttgtatga ttgggtgacg atcagagtct
     7861 tagaaaaaca aggaaaacca aaaatggaaa gaaaagagtt tcttcttgct gcgtgggtaa
     7921 caaacacgat caacaatctc gccggttttg gtggtgtggt aggagcaagt ctgcgggcta
     7981 gtttttatgg aaagcagaca aatagaaaaa tggttttagc cactgtatca aaagtcgcac
     8041 tatttatgct gactggtttg tctgtatggt cgtttcttac atttattgat gtctttttta
     8101 ttcagtcaga aagtattttt cgaagctatt ggatctggct tttcggcggt agttttttag
     8161 caccagtcat tttctttttt gcttatctaa agagaaagac attgttcttc gagttttatc
     8221 caaaaggctt gcttggtctg ctactggcat cctttggaca atggacggga gcccttgcgg
     8281 tatttttgat catcggcaag ttgatgagaa tagatatttc gatgctttcg atctatccta
     8341 tgttcatgat tgctacattg ataggaatgc tgacgatggt cccaggtgga atggggactt
     8401 ttgacgtttt gatgatctta ggaatgagtc aattgtctgt tcggcaagat gtagcagtag
     8461 tttggctatt atattatcgg ttgttttatt atgttctacc ttttatgacg ggacttctat
     8521 tgttcatcac acatacagga gtcaaaatca atcgattttt agataacatt ccgcgactgt
     8581 ttttccaaaa ggttgcgcat atcgtgctcg taagtgctgt ctattttgcg gggatcatga
     8641 tggtacttct atcaacaatc actaatctgt ctgcaatcag tcggctattt caagtattat
     8701 tgccgttctc attcaatttt ttagatcaga cagtgaatct attggttggc tttttattac
     8761 tcggccttgc tagagcactt tcagtaaaag tcaaaaaggc atttctgcct acgattgctt
     8821 tactatcttt tggtatttta aatacaatca cacgtacgtt atcttggcag ttgatattgg
     8881 tatatgtctt gattctgtta gctgtgtggt tatccagaaa agaattttat cgaacaaaat
     8941 tcgtttattc ttggggagct ataatctttg atgctagctt gttcggcttt ttgtttatcg
     9001 tttacgcaat tgcaggctat cacagcggac gctttttagt cggtcgaata acaggaaatc
     9061 attttatttt gtttccttca gatgatgtgt ggttctcagg gcttattggt ttgggcatct
     9121 cattgctagc tttagtggcg ctttatcaat atttagctga agatactacg aagcttggca
     9181 gtgagtacga aaaagaccgt tttgaccgtt tattggaaac atacggaggc acagctacaa
     9241 gccatcagct tcgcttgtca ggttacagtt atttttatta ccaagaagac gaccaagatc
     9301 aagtcgtttt tggttatcag atcaaaggaa acaaatgctt tgttttagga gatccagttg
     9361 gcaatcctaa aatgatgcgg gcagcaacga atcagtttat gaaacaagca gattgtttag
     9421 gctatcagct tgcgttttat aagatatcag aaagctacgt agtgatgctt catgaattgg
     9481 gttttcactt cacgaaagta ggtgaagcag ggattgttga tctagcaaca gaagacttgt
     9541 ctcggctgag tcagcaactt gaatatcaac gtttgatgaa agaaggatac cattttactt
     9601 ggtacgatca actgccggaa gaattacatc aagctgtaaa tgaagtttca caaaaatggc
     9661 ttcaaggtgc acgagaaaaa tacttctctg tgggacgatt tgataaatca tatgtattat
     9721 ctagcggtgt cggtattgtc cgtaaagatc aaaaagtcgt tggcttcatc accgctcagc
     9781 cgatgacgaa gaagcaggcg ggatatgatt tgttgcgggt cttacctaaa gaatctgagg
     9841 aattagctga ctatctgtta gccaatctgt ttattgtata cgagaaaatg ggctactcgg
     9901 aagccaatct aggcttagct ccattagcta atgttggaga aacagatttt tctttctttc
     9961 aagaaaaagt catgcatatc gtatataatt atggtaattt tttctattcc ttccagtctg
    10021 cttatgaaaa gaaattaaaa tatgtcgatc attgggaagg acgttatttt gcttatagga
    10081 aaggaagtaa tttcggattt gctaccatgc agctactcag tctgatagga aaagaaaaag
    10141 gaaatgttcc gagtttggca gaagaagtgc tcacagaatt ataagaatca ctagaacttg
    10201 tttggattat gaagagaaaa ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact
    10261 aggcagacat aagctatact tttatttttc cctatgatag aatacttgcg acttagactt
    10321 ttcgaatggg tgagaggatg gaattgattg aaaaagcgcc agccaagata aatttaggac
    10381 tggatgtatt atatagacgg caagatgggt atcatgagtt ggaaatggtg atgtcgagcg
    10441 tagacttggc agatcatcta ttttttgaag aaataacaga agacaaaatc attgtagaaa
    10501 caaataaagc atttttaccg gtagatgaac ggaacaatgt ttatcaagct gctttattgc
    10561 taaaaaataa atacaatatc aaaaccggtg tcaagatctt tgttaagaag aatattccag
    10621 tagcagctgg attaggcgga ggaagtacag actgtgcagc tgctctaaga gggatcaacc
    10681 aattatggaa tctgggctgt tcgttagaag aactggcaga aatcggtttg gaagtcggta
    10741 cagatgttcc ttactgtgtc tacggaagta cggcatttgt cggtggcaaa ggggaagaaa
    10801 tccaagtatt accttctatg ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg
    10861 tctctacacg tacagtgttt agagaagtag atacgcaagc catccaacat caagacatcc
    10921 acggacttgc tgaagccatc cgagagcaag attatcagca gatgatcaaa aaaatgggaa
    10981 acagtttaga agatataacg ggaaagcggc atccagtcat ccatcaaatc aaagagcgta
    11041 tgatcaagta cggagcagat gctgcattga tgagtggaag cggaccaacc gtttttgcct
    11101 tgtgtcaaca ttattcaaga gcgcagagag tatataatgg gttaaaaggt ttttgtgagg
    11161 aagtttattt agtacgaacc ctgcaataaa atataagaag taaaataggt gatgaaaaac
    11221 tgaggaatcc taaaatggta ttcttcagtt ttttattgta gtgaaatggc tagatatgag
    11281 cagcaactca tccattttac gatttaatca agaatataca tgcttaaatc agaaattgat
    11341 ggatttctat tgactgtgtt tttttatttt gatacattag ttcacgttaa taaatcgtaa
    11401 aattccgatt tgttcattga aaggagcata taaatgaaaa aatatctttt gacagctacg
    11461 gtaatgatag gtgctttact gtttgcagca tgtggaaata cgaacaagga agcagacaaa
    11521 aaagaggatt tgacgatcgt tacgactttt tatccgatgt atgattttac aaaagagatc
    11581 gtaggggatg aaggaaacgt gaaacttttg atccctgctg gaacggagcc gcatgatttc
    11641 gaaccaagtg caaaagaaag agcagaaatt tccgatgcag atgttttcgt gtataatagt
    11701 tcagacatgg aattttttgt ggattcgtta aaggattcag tggatagcaa acagaccttg
    11761 atgatcgaag cagctaaagg aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa
    11821 gaatcagaag aagggcatgg tcattcacat gaatacgatc cacatgtttg gctcgatccg
    11881 gtattagcta taaaagaagt cagaacgatt gctgaagaat taggcgaaaa atatccagat
    11941 aaaaaggaaa cttttacgaa gaatgccgat gcttatatca aaaagcttga agcactggat
    12001 caaaaatact ccgatgagct aaaagatgca acaaaccgca catttgtcac tcagcatgct
    12061 gcttttgctt atttagcgaa tcaatacgac ttaaaacaag tagcgatttc gggcgtttca
    12121 ccagatcagg aacccacacc aagtcgctta gctgagctaa aagaatttgt gaagaaaaac
    12181 aacatcaaag tgatttattt tgaagaaaat gcctcttcga aggtagctga gacattgtcg
    12241 aatgaaacag gagtgaagct agaagtgttg aatcctttag aaagcttgac taatgaacaa
    12301 atcaaagcag gcgaaaatta tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa
    12361 agtatcaatt aagttttaga atggaggttg taaggtgcgt tatatcgaaa tatctgattt
    12421 gaccttttac tatgatgatg aacctgtact agaagatgtc tcttatcatg tagatgcggg
    12481 tgaatttgtc attttaactg gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc
    12541 gttaggatta ctgaaacctt caaaaggaat gataaagatt gctgagaaaa atcaagaagg
    12601 aaaaaaatta agtatcggtt atattcctca acaagttgca tctttcaatg caggttttcc
    12661 tagtacggtg atcgaattag tccgttcagg aagatatcca agaggccgct ggttcaagcg
    12721 tctctctgaa aaagatcatc aacatgtaca gcgtgcttta gaagctgtag ggatgtggga
    12781 tatgcgaaat cggcgaatag gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc
    12841 gcgtattttt gctactgacc ctgacttatt tgttcttgat gaaccaacaa caggtatgga
    12901 tgaagaatcc agaaatgaat tttatcgtct tctccgacat agtgcgcatg tccatgggaa
    12961 atcagttcta atgatcaccc atgatcatga agatatcaaa cagtatgcgg acagacagat
    13021 ccgactggtg agaaaggagg attctcaatg gcgttgcttt catatgaatt catgagaaga
    13081 gcgtttcttg ccgctatctt cattgccgga atcgccccga tgctaggtgt gtttttagtg
    13141 atcagaagac aatcgctgat ggctgatacg ctttctcatg tctctttagc aggggtagcg
    13201 ttaggattct ttttgaacct taatcctaca cttacgacca tgctagtcgt tgtattagca
    13261 gctgtgatat tggagtatct gcgaacgatc taccgaacct attcagaaat ctccattgcc
    13321 attttaatgg ctggtggttt ggcgctagcg ttagtattga tgaatttgag cggagggaat
    13381 tcagcaacaa gtatccaatc ctatctgttt ggttcgatcg tgaccattac atgggagcaa
    13441 gtcatctttt taggaatctt attcgttgcc atcggtatat tgttcttttt attcaaacgg
    13501 ccgatgtatg ttctgacttt tgatgaagat accgcccacg tggatgggct acccgttcat
    13561 ttgatgtcga tgatgttcaa tgtgattact ggtgtggcta ttgctgtgat gatcccgatt
    13621 gccggtgcac ttttgatatc cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta
    13681 ggcaaaagct tcaatgtcgt gattgctatc agtgtttgtg taggatttct agggatgctg
    13741 tcaggactaa ctagctcgtt ttatctagat acacctccag gagcaacaat cacattagtc
    13801 tttattgctt tgttcctgat tgtaaatatc atcaaacgaa tattggttgc cttcagaaga
    13861 aaaaataact aaattataaa gaaaagaagc cttataaaaa cgaaaaattt tttataggct
    13921 tctttttatt ttaataaata ccgaactatt ttcttatttt ttcataaaat gctcgaattt
    13981 ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt tggaataggt
    14041 gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga atcacccgca
    14101 tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat cttctatcag
    14161 tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa ccttggaaac
    14221 aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg aagaggcgaa
    14281 aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc ttccaggagg
    14341 atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag ttggaagaat
    14401 cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg
    14461 cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg
    14521 ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg gatcttctga
    14581 aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt
    14641 agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct tgattgaaga
    14701 attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca acggtcatcg
    14761 cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc aaacgaaaaa
    14821 tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa agccgcttag
    14881 tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca taaatttggc
    14941 ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa atcagcgtcc
    15001 tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc cgtttattga
    15061 gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta agcctaccgt
    15121 ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt atgagtagtg
    15181 tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata ggcccgttat
    15241 ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact tgttttgttg
    15301 aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga gtgacgcagt
    15361 tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg aagtcaaaat
    15421 tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc attaatcggg
    15481 tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca gaacaagtta
    15541 aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg ggaacaggac
    15601 atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg ttagttatca
    15661 gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa tatcaccaag
    15721 gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca ggatatggac
    15781 gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac
    15841 cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac aacgaagctt
    15901 tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac tatctaacag
    15961 atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa acggatgatt
    16021 ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat gagatcatga
    16081 gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat tcagcaacga
    16141 catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa
    16201 tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag
    16261 acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc agccatgtca
    16321 agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa attggcgaaa
    16381 acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa ggaactaaag
    16441 tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg
    16501 gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc ggcgaccaca
    16561 gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa aacagttcga
    16621 ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca attgcgagag
    16681 caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac taagactttt
    16741 gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag aattctagag
    16801 ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca agattaaaaa
    16861 tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca gtcggcgtcg
    16921 agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta aacattgaag
    16981 aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca gtcaatgata
    17041 acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca aaaacaatca
    17101 acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac
    17161 caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga
    17221 ctttggacct tcatgcagtc caaatccaag gtttctttga tattccagtc gatcatttga
    17281 tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc gatgatgttg
    17341 tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg gaatttttga
    17401 aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga
    17461 atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg atcgatacag
    17521 caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg
    17581 cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa gattcagcta
    17641 tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa atcgacaaaa
    17701 tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc catgaaaaca
    17761 aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca caaaggggag
    17821 tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc ggttatgcga
    17881 gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt cctcctttgt
    17941 gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt gggtagaagg
    18001 aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag
    18061 tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa gagggacaaa
    18121 ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg gacaagttta
    18181 ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt ttgcaggcgc
    18241 agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata cgaaacaaat
    18301 tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt tttggcattt
    18361 tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatt cttggctctt
    18421 ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga tcatcgtttt
    18481 attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac aggagctgta
    18541 aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg tttccttttt
    18601 attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg catcttctac
    18661 caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt cctcttctcg
    18721 ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg ggatacgata
    18781 tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt
    18841 acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa
    18901 agattttttt acattgatct tatctgacaa gatcgaactt aatttactag caaaaatcct
    18961 ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa gatcgaagta
    19021 tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt tcatatgacc
    19081 aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt
    19141 aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg aaaagaaacg
    19201 ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat attctccagc
    19261 agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa
    19321 gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa tatgtacata
    19381 aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt attttacaac
    19441 atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa tcacgttttc
    19501 attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt atttaaagga
    19561 tgttttatga attgttttct tatggaatct acaaagtcga atacgagact catgaccttt
    19621 ttatgacgta ctggtatact tgaagaaaaa ggagagcgcg atggagataa aaaaagttta
    19681 cttttcacag aaaatgtcgc tgtattcgcc tatccagcaa gcaaaacaac cccttccatt
    19741 ttttcaaagt catcaagaaa gtagagacgc tttttggcgt tctgcaaaaa agcaatgtgc
    19801 ttttaaagca gtatcgaaaa aatataccgt ctattttact attgctaaaa gtagaggaga
    19861 tgttattttt aataatctgt tgattgaagg tgaaactttt gaatggaaaa aattttttcg
    19921 gtatatggaa gcttgcgctc gtttttttat taaagaaaac tgttgttttt tatttcaatc
    19981 tcctctatca gaagaatggt tgcgtatttt tcataaaaac ggctatcaag gaaccacaca
    20041 attaaataaa aagctggtct atcatacagc acttgtttta ggtggaggag gagcacacgg
    20101 tgcttatcag attggtgtgt ggcaggcgct gaaggaacat gatatcaact ttgagattat
    20161 cacaggtacc tcggtaggtg cgttgaacgg cgcattgatt ttacaaggag atatgaaaaa
    20221 agctgtgaat ttatggaaaa agctgtcgac aagacaagtg cttgcgttac cagaaatggc
    20281 tgcagttgaa gaccttcgtg aacgcttcta tcgagaaaga cgccaaatga cgaaaacagc
    20341 actaattgaa ggcggggttt cttctgctcc attggaaaag atggtcaaag acaatttgga
    20401 tttgtcctta ctcaaacata atcccaaaat acgtttatat acggtgtcta ctaaactgcc
    20461 agaactagcg gaagtagtga ctgatatcca acagacaaaa acagaagaaa taccagattg
    20521 gatactcgcc tctgcttcat tttatcccgc tatggcttat cgaaagatcg gaaaaaataa
    20581 atatgcagac ggtgggtatc gcaacaaaat acctattgat attgcgatta acaaaggagc
    20641 aacagaggca tttgtcgtag atgtgcaagg gccaggccca gcgaaaaaaa ttcgtatgcc
    20701 agacattttt atccattgga aatgtcaaac attgtggaca ctaggcagct ttctgctttt
    20761 tgatagccaa aggaaccaat taaatttaca attaggctat ctggaaatga aaaaacgatt
    20821 aggctattac tatgggaact ggtatacatt tcattctgtt aaacctgcaa gaacatactg
    20881 gcgaggattt ttgtcttact taacaaaaga aattcagtta gaattatctt ttttcaaaag
    20941 cataaaattt tggcaaaaac tgcgtaattt atataagaac agggtcgtac cagagacatg
    21001 cggattggcg atgctagaac ttttagcaaa aaaacatttt ttactgccca atgaaatcta
    21061 tcaagtagac atgatgattc aaatgatctg ccaacaagat ataaaaagta tgccggatga
    21121 tttattggca caaattggtc aattaagtac tgaagaatgg cgaaggtatc gaaaatacca
    21181 gcaaaaaata caaaatgagc gcacgaaaac tgtttatttt gattatctgc tacaaggaaa
    21241 acgcaaggat cgtttgcagc atgaattgct taagcaacca gttgacacat tactgatcct
    21301 atatttatat tatctcaagg aggaacaacc atggcacaag aattttcata tgaaatcgta
    21361 gaagaaatcg ctatattgtc agaaaacaac aaggggtggc gtaaagaact gaacctagtc
    21421 agctagaatg gtcgcccgcc aaaattcgat cttcgagatt gggcgccaga ccatgaaaaa
    21481 atggggaaag gcctgacatt gacgaacgaa gagtttgaac aactgcaaaa agcaatagaa
    21541 aatatgtaat gaggaaggac cctgtcgaat gaaaagtatg actggttttg gcaaagccat
    21601 ccgcgaaaca aaagcgtatc agttagatat tgaaataaaa agtgtcaacc agcgttttct
    21661 agatgttcaa atacgaagtc ctaaaatcct gaactttgtt gaaaacgatt ttcgtcagct
    21721 tattaaacag cagttaagcc gcggtcgaat cgaagtattt gtgaatctag cttatattgg
    21781 caaaaacgaa aaacagatcg tcatcaactg ggaattgatt gattcactta tcactcaatt
    21841 gacagacgga attcaacaac gatacggtag tcaaaatcaa gtgtcaatta gtcggatggc
    21901 ggaacgcttc gcgctggacg aaaacttcgt aacggtagaa gaaaaaccgg aagagagcat
    21961 ggaagaattg gcacagttag ctctagatac attgaaaagc gcacttgatt ctattgaggc
    22021 gagtcgagaa aaagaaggtc aagctttagc tttagtgttg aaaaagaata ctgaagaatt
    22081 caaagaagta ctaaaaaaac tcgatagttt tgtagaaatc tatgaaaagg aatttcaaat
    22141 aaggtatcaa aaaaagctgg aggaatttct tggagcgaaa gtcgatgagc aacggttgtt
    22201 gacggagcta gccatcttgt tggaacgcgg agatattcac gaggaactgg atcgtctggc
    22261 gatccatgta caaaaactag atgaacttat cgaagcacaa tgtcctgtag gtcgggaatt
    22321 agatttcctg atccaagaaa tgaatcggga aatcaacacg atcggttcca aatcaaatgc
    22381 aatcgaaatt aaaaatcaag tcgtccagtc taagacgatc ttagaaaaaa tcagagaaca
    22441 agtacaaaat attgaatgac gaaagaggta gagataatga aatattatga cgtaacgttc
    22501 cacgaattaa gtggaaaaac tgtggttaaa cgggatatcc cttctgaaaa aaacggcttt
    22561 gacgtatgga aagatgcctg tgcagactat aatgagaacg aactgtttat tttgatcaac
    22621 gacggtgctt acgtgacgat gaatcggaaa tttattgttc gaattgacac agaagaggta
    22681 gaagatccga cagaaaaagc acgttctcga aaagatgaga tcatgggtgt agtgaatact
    22741 ttgtccaata tgggctttta gcaaggatgc tgaacaaaga aatacaataa ataatcagaa
    22801 ttggatttct tcgcttagct tctttgctct aggaatacat ttaaaggttg tgacaaaatt
    22861 cgtgtcacaa tcttttttta ttttgaataa gtgatgttca aaaaccattt tctcggtatt
    22921 aagccaaatt tatgcaaaaa tgtgagaaac tattttcaca aaatttttct tcatactcga
    22981 aactaatcgg cttcttcacg acctcttata cacggcgttc aaaagcttgc atctgcgata
    23041 ataaccccaa ccaaacgaaa aacatgagaa gctgtttatg tttggctgtt cttattactt
    23101 gatgcaggac agctttttta cgacctcttg gtacagggta gcttttttac caatttctag
    23161 ctataattaa agataacctt gaaagttgca gaaaaaaagt gcaaaataaa cgataagtac
    23221 aaaaagacta gttgaaagga cgtcgtcatg tcagagcgtg gattattaat cgtgttgtct
    23281 ggtccatccg gtgtaggaaa aggcacagta cgaaaagcaa tttttgagag tgaggataat
    23341 gattttcagt attcgatctc aatgactacc cgtaaaatgc gtgaagggga ggtcgaaggc
    23401 gttgattatt tcttccgaag caaagaagag tttgaatcaa tgatcgaagc tggagagatg
    23461 ctggaatacg cagaatatgt cgggaattat tatggaactc ctttgtcata tgtcaacaag
    23521 acattggatg aaggaaaaga tgttttcttg gagattgaag tccaaggagc aaaacaagtc
    23581 aaagaaaaag tgcccgatgg tgtattcatc tttttgactc cgccagattt agcggaatta
    23641 aaatcacgga tcgtaggccg aggaacagat gcagatgaag taatcgaaga acgaatgaaa
    23701 gttgcaaaag aagaaatcga aatgatggct ctttacgact atgctgttgt aaatgatgaa
    23761 gtgccgctag cagttgaacg aatcaaaaac attattgcca gtgaacattt tcgagtagat
    23821 cgcgtaatcg gtaaatatat taaaatgtta aaggagatgt agtcttatga tgttaaaacc
    23881 ttcaattgac tcactattag atcgtgtaaa ttcaaaatat tctctagtga ttttagcaag
    23941 taaacgcgcg catgaattgg atgcaagtgc acagcctact ctagattctt ttgattcagt
    24001 aaaaagcgtc ggtcaagctt tagaagaaat cgatgcagga aatgtagtca atgatccaca
    24061 tcctgaatta aaacgtgaac gtttgaagat ggaagaagag gaacgtcaag cacaaaaaga
    24121 gcgtgaacaa cacgaattgg aatcacgtat ccgtgaagag caaaaaatgt aataaagaaa
    24181 aatagagcga acatctaaaa agaaaagcaa acccagctgt ttccttttca aacattcgcc
    24241 acttattgtc tggtagaaaa ggctgtgtct gaactttttg acacagttct tttttttctt
    24301 atctgctgtt aattttttgt taggatttgg cagaagataa gacaaatcca tcgatctcta
    24361 gtaaaatagg caaggaactt tttgtcgctt gataagaaaa gacaaaaaaa ggaggaaacg
    24421 aaccatgaca ttaacagctg aagtcattgt ggacgtacct actatgcaga ccgatcagcc
    24481 attcacttat ttggttccct ctgaaatgga aacagctttg caagtcggga tgcgagtaga
    24541 agtccctttt ggtaatggca atcgtcatgt ccaaggattt gttatggcta tccaaaaatc
    24601 agaagaatca gctaatccat ctttgaaggc agttattcgt ttgctagacc ttgctccggt
    24661 ggtcaatgaa gaattacttt cattagctga ttatatgaaa aaaattactt acgcattcaa
    24721 aatcacttgt ctacaaacga tgcttccgag tgtgatgaaa gcagaatatg ataaattgat
    24781 ctatcctttg gcagacacac cagaagttca ggtattgttt gatcagcgag aagtgatttc
    24841 ctggaaggaa gcagaagaag aaggcagcct ttctcaattg attcgctggc gccaagagca
    24901 attagtagat atcaaatatg aagtccatac acgaaataaa gtaaaaacga ttcgtttggt
    24961 ccgttctttg ttaactgaaa agcaaatcga agaagaatgg gcaaaattac ggcaaaatgc
    25021 caagaaacaa aaagaacttc ttttgtgttt atctgaactg agccaagaag aaccaattgc
    25081 ttatttcaaa aataaaggaa tcagtactgc tgtcttgaat caaggaaaag aaaagggctg
    25141 gctggagttt atagaatcag aacgttatcg cgacccttat aaagaccgag tatttgacca
    25201 aacgacggca ttggaactga atgcagaaca aaaaaatgct gtcgaacaaa tcatcacagc
    25261 aggtcagcag caaaaagatc aggtcttttt gctggaggga atcactggca gcgggaaaac
    25321 agaagtatat cttcaggcaa tcgctgatgt tttgtcagaa aataaaacgg ctattatgtt
    25381 agtgcctgaa atcgcattga ctccgcaaat ggtcgagcga ttcaaaggac gcttcggaga
    25441 atcagttgcg gtcttacata gcggactttc ccaaggtgaa aaatatgatg aatggcgcaa
    25501 gattgagcgg gaagaagcgc aagtagtagt aggcgcacga tctgctattt ttgccccgct
    25561 gaaaaatatc gggctgatca ttatcgatga agaacatgaa tcaagttaca aacaagatga
    25621 gacaccgcgt tatcatgcga gagatctagc aatttggcgc agtaaatatc atcattgccc
    25681 aatagttcta ggaagcgcga cgccctcttt agaatcaaga gcacgagctc aaaaaggcgt
    25741 ttatcagtta ttgcagttaa accaccgagc caaagcagct gcacagcttc cagctattga
    25801 gatcgtagat atgcgtgaag agtttcagaa tcatcggacc tctacttttt ctgctaattt
    25861 gcaggaaaaa atacaaaatc gattagacaa aaaagaacaa actgtcctac tgctcaatcg
    25921 tcgaggatat tcgtcttttg tgatgtgtag ggactgtgga tttgttcttc catgtcctaa
    25981 ttgcgatatc tctcttacac tgcatatgga tacacgttca atgcgctgcc attattgcgg
    26041 acatgaagaa cctattccca atcgctgtcc taattgtggc ggcaataaaa tccgctacta
    26101 tggaacaggt actcaaaaag tagaagagga actccgagaa ctttatccgc aagctaggat
    26161 tttgcgtatg gatgtagata ccactcgaag aaaaggggca cacgaacaga ttttacaaaa
    26221 attcggtgca aaagaagcag atatattatt agggacacaa atgattgcta aaggtttgga
    26281 ctttccagaa gtgacattag ttggggtgtt gaatgctgat acctccctga atttaccgga
    26341 ttttcgttct agtgagcata cattccaatt gcttacccaa gtttcaggac gagcagggcg
    26401 agcggaaaaa gcaggcgaag tcgtgatcca aacattcaat cctcaacatt acgccataga
    26461 attggctaaa aaacaaaatt atgagcagtt ttatcagcag gaaatgcatg tacggcatcg
    26521 tggaggctat cctccttatt actttactgt gaaaataacc gccagccatc ctgaagaaca
    26581 agtagcggca aagaaaatct ttcagattgc caatcagcta aaagaagtgt taagccctca
    26641 aagcttgcta ttagggccaa cgcccagcat gattttgaga gtgaaaaacc gatattatta
    26701 tcaactaatc atcaaatata aacatgaacc gaatcttcct catgttttgg atgaaatttt
    26761 gaatggaagc cagaaagaac agcgacaagg tctttttgtg gcaattgaca acgaaccatt
    26821 aaactttatt taagaaaggg ccaaattatg cgctatccta tattgatcca ccctaatgat
    26881 aaattaaaaa gaactgctca gccaatcgat gtaatcacgg acgagacaat cgctttatta
    26941 gacaatttat atgagacaat gattgcgaat gacggaatag ggatcgctgc tcctcaagta
    27001 ggacaaaaca agagaattgc tgtgatcgaa gtagatgaag gcgagaaatt tgaactgatt
    27061 aacccagaaa tcatcgaagc aaaaggtgaa agccttgacg tagaaggttg tctcagtatt
    27121 ccgcatgtct atggaacggt caagcgagca gatgaagtga ccgtgcgtta ttatgatcga
    27181 gacggagaag agatagaagt gacggctttt ggctatttag cccgtgcctt ccagcatgaa
    27241 atcgatcact tagacggtat cttgttcatt gagaaaatga tacagcagat cccggaagaa
    27301 gaacttgaag aatatatgga ggaacatctt gatgactaag ctagtattta tgggcacacc
    27361 tgctttttct gtgccgattc ttgaagggct gatagaatca ggttacgaga tccaggcagt
    27421 ggtaacacag cccgaccgtc cagttgggcg gaaacgagta attacgccga caccggtaaa
    27481 agaagcggct ctgaagcacg gtatccgtgt gctgcagcca gagaaaatct ccggttcgcc
    27541 agaaatggaa gaaatcattg aattggtgcc tgatgtgatc gtcactgcgg catttggcca
    27601 attcttgcct gaaaagttat tgcaagtacc aaaattaggg gcaatcaatg tccatgcttc
    27661 tttacttcct aaatatcggg gaggtgcgcc tgttcattat gccatcatga atggggagaa
    27721 agaaactggt gtaacgatca tggaaatgat caaaaaaatg gatgcaggcg gaatctatgc
    27781 gcaagaaagc ataccaatca ctaaacaaga tgatgtaggg acgatgtttg aaaagcttag
    27841 ccttttagga agaaagctat tgctagaaac attgcccaat attttggatg gacaaaaacc
    27901 agtacctcaa gacgagtcag aagcaacttt ttcgccaaac atcacacggg aacaagaagc
    27961 aatcgattgg cataaaactg cagaagaaat tgacaaccaa gtacgcggaa tgcgtccttg
    28021 gccaattgca ttcacgacct atgagggcat acgctggaaa ttattatcgg ttgaagtatt
    28081 ggcagaaact acagacaaag cacctggtac gatcatccgg aaagacaaaa aaaatctatg
    28141 gattgcttgc ggtaaacaga ccgtcttagc catcaatgaa ttacagccag ctggtaaagg
    28201 aaaacaggcc gtacatgatt tccttaacgg aagcggtcag caagttcaga ttggtcagca
    28261 ggtgaaataa tgaaaaaaat ccctaaaaaa atgaaaaaat cagtacgata tatggcatta
    28321 ctgacattag agcgagtgaa caagggtggc gcctactcga atcttttgtt gaatgagatg
    28381 atcaaacaaa gcgagctaaa tcaaaaagat gtgggtttat ttacagaatt gatctatggt
    28441 acgattagcc atcagcgact attggaattt tacgttgcac cattgatcgc taaagcaaaa
    28501 aaagtggatg actgggtcaa gacgcttctt tatctttctg tataccaatt agaattttta
    28561 gataaagtcc ctgcacatgc tattttaaac gaggcggtcg aaatcgcaaa agtaaaagga
    28621 aatcctggga cagggaaatt tgtaaatggt gtcttgagga attatcagcg tcaaggagca
    28681 ccagatctgc aaggcattgc tgatcctatc gaaagacttt ctgtcaaaat cagcctgcct
    28741 ctatggatga ctaaacgact ggtggatcaa atcggatacg aagaaactga aaagctcggt
    28801 ttatcgctat atcagcctag ccatgctagt gcccgagtag atacacgccg actttccaga
    28861 gatcaggcca tcactgtttt gcaagaagaa gagatcacag ctgaaaaaag ccaagtcagt
    28921 ccatacggta tagtagcgaa aaaagggcat ttagcttcta gctcattgtt ccacgatggc
    28981 gtcatgacga tccaagatga aagttcgatg cttgtagcgc cgtctatgca aatcgagcct
    29041 gaacatcatg tcttagatgc ctgtgcagca cccggaggta agacgacgca tattgcttct
    29101 tttttagatc cgtcatgcgg tggagaagtg gtcgctttag atgtacacga acacaaagtc
    29161 aaactcattg aagataatgc agcacgtcta ggtgtctctt ctgctgtcta cgctcaaaag
    29221 atggatgcta gagaagtcag taaacagttc gaagcagagc agtttgatcg gattttagta
    29281 gacgcgcctt gttcaggtct tgggctgctt cggcgaaaac cagatattcg ttataaaaaa
    29341 cagccggatg aattagcgaa tttaccaaaa atccaattgg aaattttaga aagctgcgca
    29401 ccaactttaa agtcttccgg tattctgact tatagcactt gtacgatctt aaaagaagaa
    29461 aaccaagacg ttgtttctgc tttcttggaa agacaccctg attttgagag gattgatgtt
    29521 ttgaccaatc ctatgctaca atcatcaatc gaagagaaaa tgttgacttt gtatccgcat
    29581 cagttttaca cagatggatt tttcatttgt tgtttgcgca aaaagtaatg aggtgaaaaa
    29641 atgcagattg aatatcaatc agatgtagga agacgacgta acacaaatca agactatgcc
    29701 agtgtattta ccagccgttc aggtctcaag cttgctgtat tagcagacgg gatgggtggt
    29761 catcgtgccg gagacattgc cagtcagatg gcggtaacga atctaggcga agcttgggaa
    29821 aaacaagaag ctgttgacga tgaaaaaatc gcccaatggt tcattcaatc gattcaagaa
    29881 gaaaatatgc tgatttatca gcgtggacaa gaacagccgg aatataacgg tatgggaaca
    29941 acaatcgtag cggccgctct gtcagatgac tggttcacga ttgccaatgt tggggatagc
    30001 agagcttatt tgattcgtga agataaaatc atgcaattga cggaggatca ttctttagtc
    30061 aatgaactag tcaaatcagg agaaatcagc gaagaaatgg cagtgaacca tccaaggaaa
    30121 aatattttaa cacgttctgt cggcatgcct ggaacagtcg aagtagatgt ttctactcat
    30181 ctttggcaac taaaagatcg actgctgtta tgttcagatg gcttgacgaa catgctgtct
    30241 gaagaagaaa taggaacgat catcaatcaa gaaggcccat tgtctcataa agtaactgag
    30301 ctgattgatc gtgccaatga agcaggcgga gcggataata tcaccgttct tcttatcgaa
    30361 tataaggagg acagtgtatg atcgagttgg gcaaaaaatt aaatggccgt tatcaaatca
    30421 ctggaaatat cggtagcggt ggtatggcaa atgtattttt ggcgcacgat ttaatcttgg
    30481 atcgtgatgt agcagttaaa gtcttacgat ttgactttca aaatgaccag actgcgattc
    30541 gacgatttca gagagaagct ttagcagcaa cagaactcgt tcatccaaac attgttagtg
    30601 tttacgatgt tggtgaagaa gacaacatgc agtatctggt gatggaatac gtcaaaggaa
    30661 tggacttgaa acgatatatc caaacacatt atcctgttcc ttatgaaaca gcagtaaata
    30721 ttatgcagca gatcctttct gctatttctt tagctcataa ccatcaaatc atccaccgag
    30781 atttgaaacc ccaaaacgta ctgatcgata atgaaggtgt tgtaaaaatc acggatttcg
    30841 ggattgctat cgctttatct gaaacatcga tcacgcagac gaatacgatg ctaggatctg
    30901 ttcattatct gtcccctgag caggccagag gaagtatggc aacgaagcag tcagatattt
    30961 atgctttagg gatcatcctt tatgagatgt tgacgggtag cgtaccgttt gatggtgaat
    31021 ctgctgtaac aattgcttta aaacattttc aagatgatct tccatctata aaagcacttg
    31081 atccaaatgt gccacaagca ctggaaaacg tgattttacg ggcaacggct aaagaacctg
    31141 cagaccgtta taagtcagcc gaggaaatgt ctgatgatct ttctacagta ttatcaccag
    31201 ctcgtgcaaa cgaagaaaaa tggcagcctc atgtgatgga taatgaaaca aaagttatca
    31261 caccccttac ggaagatgca ccaatgcctg attcattcaa atctatgcct ctgccaaaag
    31321 acaagccaga aaacgaaacc ttaccagagg aggaagaagt tctgcctgat cctaaaaaga
    31381 aaggacagaa aaaatggtgg ctgatcctat tagctattct tgctgtttta ggaatcggaa
    31441 cagctgtatt ctttgcttct ggcgggcgag gagaagttgt tgttcctgat gtttctgatc
    31501 tttctgaagc ttctgctaga gaaacactcg caaaagcgaa attagaagta gcaacaaaaa
    31561 cagaagaaat tgcagatgac aaaatcgaag aaggtaatgt agtgaagaca gacccggcag
    31621 cgggaacgac agtcaagcaa aaacgcgaag tcactctgta tattagtagc ggcacgaaaa
    31681 aaatcaaatt agatgattac agtggtaaaa gttatgaaga tgccgaagca agtttgaaag
    31741 aattagggtt ttctgctgac ttgattcaaa aagaagagga attcagttca gaggtagaaa
    31801 gcggaatgat catttcgcaa tctccatcag aagatacaga agtcgatcct aaaacagaca
    31861 cgattacttt cacagtcagc aaaggaccag aaacagtgac tttgagtagt catgtaggag
    31921 aaaactacga aactgttcgt aattggcttg ttacacaagg attcaatatc aataatatta
    31981 gtgctactta tgactatagc gacactgtac catcaggttc gatcatcagt caggatccag
    32041 cctcgggtga agttgtagca gaagaaactt acatcaattt tgtcgtaagc caaggaacag
    32101 agcctaaatt gaatgatatc tccggctata caaaaagcga agcacaaagt tatttagcta
    32161 gtgtcggtgc agaatatatc gggcatgaga cttccgaata ttcaaacacg gtggataaag
    32221 ataaagtgat tcgtacagta ccaggggcag gaacgacaat taccaaagga atggttgtga
    32281 acgtaattta ctctaaagga ccagatccag cgtcttctag ttctgaaaaa gactcttcag
    32341 acagttctag cagctcaagt agctccagta gcacaagcaa ctcttcaaaa gaagaatctt
    32401 ccagctccac aagttcatca gctagcacct cagaaagttc aaaataagtg ctgaataaaa
    32461 tatatcagaa gggaatgcga cattgatttg ttgcattctc ttttttgtgt ttctatctct
    32521 tttgatcaat tttcaagcca tatactttga gaagtttgca tggaataaca cctactttcc
    32581 tcttcgtatg gtaaaataaa caaaaacgga ggtggcactt atcaaaggac aaatcagaaa
    32641 agcaatcagc ggtttttact atgtatacgc aaacggagag acctatcaaa caagaggaag
    32701 aggaaatttc cggaatcgga aaatcacgcc gcttgtcgga gatcaggtga tctttgagag
    32761 tgaaaatcaa acagacggct atcttctgga aatcttgccg agaaaaaatc aattagttcg
    32821 cccaccagtc acaaatgttg atcttggaat cattgtcaca agtatcgtag agcctgcatt
    32881 ttcttataat ctacttgacc gctttctagt taccttggaa tacgagtcga tcgaaccgat
    32941 tatttttttg acaaagaccg acttgtcaga cgacccagaa aaaatcgaag agatcaagca
    33001 aacctatact aaaataggct atccggtgat tgtacccaat catacgggtg atacgacaga
    33061 actgatccgt tacttcccag aacggctatc ggtctttatg ggtcagtctg gagcaggaaa
    33121 atcgacgcta ctgaatcaaa tatcaccgga actgaattta gcaacaggag agatttctga
    33181 tgcacttgga agaggacggc atacaacacg tcatgtggaa ctgattccta tctatggcgg
    33241 gttagtagca gatacaccag gatttagctc gattgacttt ttgacaattg aagcaggaga
    33301 actgccgaga caatttcctg aatttgtaga agcttctgtc cattgcaaat ttagagagtg
    33361 catgcatgct aaagaacctg gctgcgaagt aaaacataga gtagaaacag gtggtatcgc
    33421 acagacacgt tacgacaatt atttgcaatt cttacaagaa atcgaaaaaa gaaaaccaat
    33481 gtatgcaaaa aaagaaggta aacgaaaata ggaggaaaca agaatgaaga tagcaccatc
    33541 cattttgagt gcagattttg ccaatttaca aagagacatt gagttagtgg aaaaaggagg
    33601 cgcagattac atccacgtag atgtaatgga tggccaattt gtcccaaata tcacattagg
    33661 gccaaatatt gttcaggcaa tccgtcctgt tactaaatta ccgttagacg tccatctgat
    33721 gatcgttgat ccagaaaact acattccagc atttgcaaaa gctggagcag atatcattac
    33781 tgtccacgtc gaagcaactc ctcatattca tcgggcactt caaatgacaa aaagcttagg
    33841 agttaaagct ggagtagtta tcaatccagg aacaccagtc tccatgatca aacatgtttt
    33901 gcctttggct gatcaagtac tagtcatgac tgtcaatcct gggttcggcg gacaaagctt
    33961 tattgaagaa acagtagaaa aaattacgga actcgctgat ctacgtagag agaacaattg
    34021 gacttatgca atcgaagtag atggcggaat cgttccagaa acagcaaaaa tctgtaaaga
    34081 tgcaggggcg gatgtcttcg ttgcagggtc atatatctat ggagccgaaa atccagtcgg
    34141 acaaatcgaa cgattaaaag aggcattaga ctaaatgaaa attttattag ctgctggagg
    34201 taatccgcaa aactggccgc agtctctgct gtccaaatac gatgcctatg tcgggataga
    34261 ccgtggtgcg ctgtacctgc ttcaagcagg ctacccagta gatctggcag tgggtgattt
    34321 tgattcattg tcgaacgaag agcagaagga agtatttgca aaagccaaag agtatacccg
    34381 ttcccctgca gaaaaagacg atacagatac acagttaggt atcttaaaaa catttgaacg
    34441 ttacccgcaa gcggaagtca ctttgattgg agctacgggc ggaagattgg atcatctttt
    34501 agccaatctc tggttaggta tggaacctcg cttcaaaccc tttgcccatc agcttattat
    34561 cagagattgt caaaattatt tgtcttatta tttgcctgga gagcatatag taagacagat
    34621 accagatatg aagtatttag cttattgctg cttgactccc gtctcgaatc ttacattaaa
    34681 aggcagcaag tatgtattgg aaaataaaga agtagaatat ccgacatctt atgcaagtaa
    34741 cgaatttctt tcagataccg cagaattttc tttttcagat ggtatgattg cagttattca
    34801 aagcaaagat tgatccataa acaaataaaa aaatttgtct ttcaggctaa aaatgacaga
    34861 atctattatt tgctaaaaaa aagcttaaga ggagcaactt accttttgtt tcacggcatt
    34921 tagtgataaa attgaacaca agagtctaat taattcgagg tgtctttgtg aagaagttca
    34981 atcctaataa aaatattatt attaccttgg ttattgttat catcgtggtg acgatactaa
    35041 gtgtgaccgc tgcgcagcga gcgaatgaag gaaagcccaa tttcttccaa tccatcgtta
    35101 atgacagtgt tgcaatggtt gataaaacaa tctctgcccc agtacggtgg gtacgaaatg
    35161 gagtagattc tgtccataat cttttttcaa cttatgccga aaatgagcgg ctaaaagaaa
    35221 aaatcgatag ctatgatgac gtttcacagc aaaacaagaa ttacgagaga gaaatcgaag
    35281 cgttgaaaaa agaacttgag ttgaatcaga cgctgactag ttacgaaaaa gtaacggcaa
    35341 atgtcattac tcgttcgcct gacagttggc aggacatgct gatcgtagac aagggttcta
    35401 aagacggtat cgaagcaaat atggcagtca tgtcgcaaaa aggattgatt ggccgagtga
    35461 tcgaggtcaa tacggcttcg tctaaaatcg aattactgtc atcctctaat gaaagctcca
    35521 atcattttcc agtacgggta tcttcggcta atggcgaagc gtttggtttg cttaaaaact
    35581 atgatgaaaa gctccatgcc ttagtggtga cccaattaac tggtgatacg gatatcaaag
    35641 aaggggatgt tgtccagaca tccggtcttg gagggaattc tccagctaac ttgccgatcg
    35701 gtacggttat taaaacgaaa ccagatagtt atgggctgga tcgggaagtt tatgtgaaac
    35761 cttatgcaga aatgtatgac gtgtcagttg tgacgattgt ccaacggcta gcggaggatt
    35821 agtccatgtt gaagaaagaa acaatgaagt actatctgcc aatcgttttg ttctttttga
    35881 tgttgataga tggtcattta acaagaatgc taggggagtg gtcgaaaggc acctatatgt
    35941 caaatgccca ctttctgata ttggcattat tatgttgcag tatggcgttt gaaaaacgtt
    36001 atttactgat taccacgatt gttctcgggg ctatctatga cgcttactat attggcgtta
    36061 tcggtatcta tgcagtagct ctccctttaa ttgtatggtt gatgtatgta atgaaagacg
    36121 ttatccatgt caacatcttt actgaatttt tcagtatgat catctttgtc acgggttatg
    36181 aattgtttac gatggtggtc cagttgattt ttaaattagc agtagtaaat aacacgtatt
    36241 ttattacaag gtttttagga cctacactgc tgttgaacat gattatattt gtattattca
    36301 tttttccctt taagaaatta ttcagtgatg aataatttct ttttttattt ttaggtcgaa
    36361 agcgaaaaag aatcgttgtt atcttaagaa atgctagact taacgcggct ttttcatgat
    36421 cattttctga aacgaacagt tttgtcattt ttaaaagttg ttttgaaaca tttttgtaac
    36481 ataactatta tatcgttgac acactgctat ggtacaattt ttatatcgtt aagaaagact
    36541 taaatatatt tagatattta ttaaattaag ccagtcggag gaatgaagag tgaaaaagag
    36601 tttaatatca gcagtaatgg tatgttcaat gaccttgact gccgtagcat caccgatcgc
    36661 tgccgcggcg gacgattttg attctcagat acaacagcaa gatcaaaaga ttgcagactt
    36721 gaaaaatcaa caagcagatg cacaatcaca aatcgatgcc ttagaaagcc aagtttctga
    36781 aatcaacaca caagcacaag acctattagc aaaacaagat acattgcgtc aagaatctgc
    36841 acaattagta aaagacattg cagatttaca agaacgtatt gaaaaacgtg aagatacaat
    36901 ccaaaaacaa gcacgcgaag cacaagtaag caacacaagc tcaaactaca tcgatgctgt
    36961 attgaatgct gattcattag cagatgctat tggacgcgtt caagcaatga caacgatggt
    37021 aaaagcaaac aacgacttga tggaacaaca aaaacaagat aaaaaagctg tagaagacaa
    37081 aaaagctgaa aacgatgcta aactaaaaga attagctgaa aaccaagctg ctttggaatc
    37141 tcaaaaaggc gatcttttat ctaaacaagc tgatttgaac gtactgaaaa cttcattagc
    37201 tgctgaacaa gcaactgctg aagacaagaa agctgacttg aaccgtcaaa aagcagaagc
    37261 tgaagcagag caagctcgta ttcgtgaaca acaacgtcta gcagaacaag ctcgtcaaca
    37321 agctgcacaa gaaaaagctg aaaaagaagc acgcgaacaa gcagaggctg aagcacaagc
    37381 tactcaagct tcatctactg cacaaagctc agcaacagaa gaatcatcag ctacacaatc
    37441 ttcaatgact gaagaatcat catcagctac acaatcttca gcaacagaag aatcaacaac
    37501 acctgaatct tcaacagaag aatcaacagc gcctgaatct tcagcaacag aagaatcaac
    37561 aacagtgcct gaatcttcag caacagaaga atcaaaaaca gtgcctgaat cttcaacaac
    37621 agaagaatca acaacacctg cgcctacaac accatcaaca gatcaaagtg ttgatacagg
    37681 aaacggcaca ggaagtagta ctccggctcc aacgccaaca ccaacacctg aacaaccaaa
    37741 acctgtaaca cctgctccag ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga
    37801 agcttacaaa tatattggta ctccatatgt ttggggcggt aaagatccaa gtggctttga
    37861 ctgctcaggg ttcacacgct atgtttacat gcaagtaact ggtcgtgaca tcggtggttg
    37921 gacagttcct caagaatcag ctggaacaaa aatttcagtt tctcaagcaa aagctggaga
    37981 cttgttattc tgggggtcac aaggcggaac ttaccacgta gcgattgctt taggcggagg
    38041 acaatatatc catgctcctc aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt
    38101 tgcccctgac tttgctgtca gcatgtaaga atgattaatc aaaaagagtt agcccattct
    38161 ggactaactc tttttt
//
LOCUS       DACCDF010000020        36937 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCDF010000020 DACCDF010000000
VERSION     DACCDF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.020"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(93..521)
                     /locus_tag="IVK51_000995"
     CDS             complement(93..521)
                     /locus_tag="IVK51_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430604.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(549..1187)
                     /locus_tag="IVK51_000996"
     CDS             complement(549..1187)
                     /locus_tag="IVK51_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430605.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1191..3227)
                     /locus_tag="IVK51_000997"
     CDS             complement(1191..3227)
                     /locus_tag="IVK51_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP6430606.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3353..4018
                     /locus_tag="IVK51_000998"
     CDS             3353..4018
                     /locus_tag="IVK51_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430607.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(5953..9102)
                     /locus_tag="IVK51_000999"
     CDS             complement(5953..9102)
                     /locus_tag="IVK51_000999"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP6430608.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(9115..10314)
                     /locus_tag="IVK51_001000"
     CDS             complement(9115..10314)
                     /locus_tag="IVK51_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP6430609.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(10304..11899)
                     /locus_tag="IVK51_001001"
     CDS             complement(10304..11899)
                     /locus_tag="IVK51_001001"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6430610.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(12483..13190)
                     /locus_tag="IVK51_001002"
     CDS             complement(12483..13190)
                     /locus_tag="IVK51_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430611.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(13255..14091)
                     /locus_tag="IVK51_001003"
     CDS             complement(13255..14091)
                     /locus_tag="IVK51_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6430612.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(14109..15518)
                     /locus_tag="IVK51_001004"
     CDS             complement(14109..15518)
                     /locus_tag="IVK51_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6430613.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(15532..17355)
                     /locus_tag="IVK51_001005"
     CDS             complement(15532..17355)
                     /locus_tag="IVK51_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6430614.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(18198..18524)
                     /locus_tag="IVK51_001006"
     CDS             complement(18198..18524)
                     /locus_tag="IVK51_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430615.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(18570..18932)
                     /locus_tag="IVK51_001007"
     CDS             complement(18570..18932)
                     /locus_tag="IVK51_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP6430616.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(18969..19184)
                     /locus_tag="IVK51_001008"
     CDS             complement(18969..19184)
                     /locus_tag="IVK51_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430617.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            19311..19646
                     /locus_tag="IVK51_001009"
     CDS             19311..19646
                     /locus_tag="IVK51_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430618.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(19722..21719)
                     /locus_tag="IVK51_001010"
     CDS             complement(19722..21719)
                     /locus_tag="IVK51_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430619.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(21817..22092)
                     /locus_tag="IVK51_001011"
     CDS             complement(21817..22092)
                     /locus_tag="IVK51_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430620.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            22324..23058
                     /locus_tag="IVK51_001012"
     CDS             22324..23058
                     /locus_tag="IVK51_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430621.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            23097..24239
                     /locus_tag="IVK51_001013"
     CDS             23097..24239
                     /locus_tag="IVK51_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6430622.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="IVK51_001014"
     CDS             complement(24334..25725)
                     /gene="sufB"
                     /locus_tag="IVK51_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP6430623.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(25730..26200)
                     /locus_tag="IVK51_001015"
     CDS             complement(25730..26200)
                     /locus_tag="IVK51_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP6430624.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(26187..27422)
                     /locus_tag="IVK51_001016"
     CDS             complement(26187..27422)
                     /locus_tag="IVK51_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6430625.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="IVK51_001017"
     CDS             complement(27419..28705)
                     /gene="sufD"
                     /locus_tag="IVK51_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP6430626.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="IVK51_001018"
     CDS             complement(28718..29491)
                     /gene="sufC"
                     /locus_tag="IVK51_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP6430627.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(29688..30536)
                     /locus_tag="IVK51_001019"
     CDS             complement(29688..30536)
                     /locus_tag="IVK51_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430628.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(30560..31246)
                     /locus_tag="IVK51_001020"
     CDS             complement(30560..31246)
                     /locus_tag="IVK51_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430629.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(31239..32273)
                     /locus_tag="IVK51_001021"
     CDS             complement(31239..32273)
                     /locus_tag="IVK51_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430630.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(32668..33006)
                     /locus_tag="IVK51_001022"
     CDS             complement(32668..33006)
                     /locus_tag="IVK51_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP6430631.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(33008..33364)
                     /locus_tag="IVK51_001023"
     CDS             complement(33008..33364)
                     /locus_tag="IVK51_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP6430632.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(33370..34557)
                     /locus_tag="IVK51_001024"
     CDS             complement(33370..34557)
                     /locus_tag="IVK51_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6430633.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPVRS
                     SRR"
     gene            complement(34748..35593)
                     /locus_tag="IVK51_001025"
     CDS             complement(34748..35593)
                     /locus_tag="IVK51_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6430634.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            35827..36792
                     /locus_tag="IVK51_001026"
     CDS             35827..36792
                     /locus_tag="IVK51_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP6430635.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        10953 a         7583 c         6122 g        12279 t
ORIGIN      
        1 caactaaaaa tgaatatatt aaaactacca aaaataactt ttaacgctgt ttcattatat
       61 gttttttcta ttttatataa acttttggat acttaatacc aaccggttgg attgtaccaa
      121 catgagaatg tgtcagttct atttcctgtc gcacttgcat aggcccatac tccttttgtt
      181 gcatttgctg atcgaataga gtttccaact ttcgcttttg caccgtgacg attatagaca
      241 cgactatagt ggttagaatg acctgttttt ctcgtacaag ttgtatcact ataataaacc
      301 catcttccag agctatgatt aattgctgtt ctagaaattt cccggtctac tggtaaattt
      361 gacttaataa tattaccttt agcatcaaaa tgaatctctc ctggctttaa agatttgtaa
      421 tagatttcac cgtgaactga atttgcttcc acttcagatg acatagcgtt tcctccaaca
      481 aaacctatcc ctagtgcaca aataataact gcttttttca taaaacgaca tcctttcctt
      541 tttgattttt aaagcatgat cttcttgttt cctattttgg tcagatatgg atcatgcgtg
      601 actacgataa ctaacgtacc ttgggaagct tgttgcgata agatttcaac tactttatcc
      661 ttatttcttt tatccaatga cccagtcggt tcatcggcta aaatgatttt gctattcttc
      721 aataagactc tcgcacaggc gattctttgc ttctcaccac cagataatgt gtagataggc
      781 gtacttagct tcttgtttat atttactttt ttcaatgcct catactttgc tgttcgctta
      841 tctgaaacat ttaaattcga aaacttcaat ccaatatcta aattaaaatc gattgtttca
      901 tattcaatca acccaaaatc ttgaaaaaga aaagatatgg tatttctttt tatcaagcgc
      961 ttctctctcc cagttaattg tttcgtctct tgatcgaaca aatagaattg tcctgaactg
     1021 aactgatcat tcaatcctaa tatattcaat aacgtacttt tacctgctcc actttctcca
     1081 acaacagtca caagatcccc agcatccgca gtgaaacgat ccaaatgaag aatcgttttg
     1141 tttcgaatga tttttttcgc accttttatc actatcaacg gctccaattg ttaacctcca
     1201 ttcatcagca ataactgact tttcttttct gtagacaaaa taaaaacaac tgaaagaata
     1261 aaatcaatga agctaaagaa aaaaatatta aaaagatatt taggctctct ttctataaga
     1321 aaaaggaaga ttacacttgt cgttccccat ttgatgaggt tcatcatgaa gaaatcttta
     1381 tacgtttcaa agagcgaata accacttgaa cgaaagatag cgattttccg acaattgtag
     1441 acaaaatata tgcataacat gacatagctc aacaagaaga aagaaaagaa agtgagtaaa
     1501 aatatctgta tcaagtaatg aaatgatctg cttaacactt tgacagctat ctcttcagtc
     1561 tgagtatacg gaaatgataa ctgcaagtta tcacgtaact gactttcttc aatcagctca
     1621 aataaccttt ctttcgacaa agagcctatc ttgatcttta acggtggata gatttctccg
     1681 ctgagaatat ttctttccaa atacatactg tttttcaatg tcagtatttc aacaaccgga
     1741 taatgttcga tccaggggat agaaggaata aatgtataaa tgggttgatt gttttttata
     1801 aatagaaatt tcggttcttt ttcactgatt tcttgataat attctgttat ttgcgccagg
     1861 tcatttttta aatctgtatg tctatatcgt tcagggatca atataacagg gtgcgtttct
     1921 ttttcggtta tttggatcaa ccttccagag acatcaatca aaggaaattt cttaagataa
     1981 ttcagattta tttttatccc tctgccaaac tgttcatttt cttcattcaa gtaatctgat
     2041 acattgacta atatacttcc gtttttattc aaagtttcat ataattcctg atctgctgct
     2101 gatgcaaaac gattggaata aataaaatcc acgtggtttt tcccgatggt tactggataa
     2161 aatacatgat agtcctcacc agcgatttct tttggtggtt catgggaaac agctaactgt
     2221 gaagaagaaa aaacgatagt tgctaattcg acattggcaa cggtcaaaag aagagaaaac
     2281 aacaatccaa atgtaaaaag aatgtattgg atatagttga acgcttctct taacgaagcg
     2341 attttttcaa ctaataaaag agaaatcaaa gagataccat atattccgct gaacaagaaa
     2401 acgtacatct ggaacaaata aagaaattgc tccttataat acataaaaat aaaaccacca
     2461 atgattcctg caattatgaa tgtaataatg attttctttt tccccaataa acaccacaat
     2521 acttgatgta atgaaaagtc attattcaac aataaactga tttctttatt catcaataag
     2581 aaaaaggatg ataacaaaag gaataaaaga atcaatccaa tagttaaata cactcgataa
     2641 ttatatacgt ctgtatttga aataaccgaa tacgaatagc tattttcaaa gtcttcaaaa
     2701 atattagtgg cataatcagt gtgaaatgct tgattgaatc gatcagtgaa tagtgaaatg
     2761 acccgttcat aggcttcttt ttctctcgtc atcaagaaaa attcaccttc aaaatctttt
     2821 attttgatcg attcttcaat ggacgttacg cgaaaggagg tattagaata gggtaccttc
     2881 tcgtgagaag atttgacatc cccttgataa tacaatgtca tttcaagctt tggctgtatc
     2941 ttgaagttcc aagatgtatc tacttcattg gattcattta ctgttttttt catataagaa
     3001 agctcagttt ctgatgagac attttcaaat accatgatta gctgctcata ttcttcttgc
     3061 ccaataaaat gttgtggcaa tcgaatcgga gtataagtat ctgtttgatg gaaaaattca
     3121 tttgtccgat agagtacctg tttatttata ctaaccaagg caaaaccact catgataccg
     3181 gtcactatga ttgtgagtaa aactgtgaac ttttttttga ttatcaccac tctcccttac
     3241 ttaaatgatt ctttgataaa atcataccgt ttttcatcaa aaaaacgtat ctatcaagtg
     3301 tataggtcat gtagaataaa tctatatact gtagaatcga aaggagaaga agatgaacac
     3361 cggagaaaaa atacgaactt accgcagaaa ttgtaagctt acgcaaaaag aattaggcga
     3421 aaaaattggt gtttccgata aaacgatttc tagttgggaa aattctcgta caatgccaga
     3481 ctttgaaatg ctgtcacttt tacatcatac tttaggtctg ccgattgatt ttccttctat
     3541 tgaatcagct accgaatcaa tagaaccaac attgccagat gaacctcttg ttaacaagaa
     3601 atcttattca tcaaaacaac ccttctcact caaaaagaca ctatctttct ttctgctcgt
     3661 cttttgtttg gtctatcttt ttcttatcaa ttatcaatac aaaaatgaaa cgatcgatcg
     3721 attcaatcct tttttgaaac cacaaatcgg ctataccaca ctccctgtag gatacgtgga
     3781 caattccatc gaacgttggg tatgtaatga tatctttggc catggtacat ggctgtcttt
     3841 tcacgctgga gaggtagaaa aagagaaaaa ccatgctgta atcaaacaca aaggaaaaca
     3901 cgtcatgaac gttcgcctgc tcacttggga agccattccc tcagattacc aagaaatcct
     3961 cagccaagac tatgacgacg ccttaaaaag aagccctcag gagccagata ggcattaagt
     4021 aggagatgaa cgtagagata gaaaaaatca atgcacatga tcttatccaa aaaacagggg
     4081 tacaaattaa ttgaaaaagc cttgagatga aagttcctaa ataaaaaagt tcagaatgat
     4141 aggtatttct ggcttatagt ataatattta cgccacagcc tcccaatgat tctttttgga
     4201 atcataatca aggatggaca ttcacaaaaa tattttgctc attcgatccg aatagattaa
     4261 cgaccaatct agtaagtaat atagattggt acgtagtcaa gctgcacata atttagatca
     4321 attttagttc ttttttatat ttctatgatc tgctcattta cttttatatg atggcttaca
     4381 gtaacataaa taaatatgat aatcgctggt agtttatcca agagtactca ataaaataca
     4441 aaatggattg ttcaagcgcc tatcataatg ataaaatgaa acaaataaac gatacaagga
     4501 ggaagagact ttgagtaatg gcagaacaat tttttcattg taaaaattgt tcagttgtta
     4561 atctatggga tttcggtagc atgggcattt ttttatagag gaatcacacc aacctggccg
     4621 ttttatctct tgttagtggg aatattagta ggaaattttg cccctcttca ctatcgtacg
     4681 aacttttttc caactgattc tggccttttt cttaaaacaa agacgcgaaa attagaaaac
     4741 attattgaaa tgacaattac ctgtataatc attttcttag ttatattcct aaacgtcttc
     4801 taataagaat ctcttaaaat tagcttgtta cagttagtta cctagtttat ataacacagt
     4861 tatcttatct tccttatcaa catcttttgc caactctttg tcttaaattc gaaacatata
     4921 agcagagcta cgaacaccaa gactgtttcg gctctgtttt ttgcatcaaa ttcactctta
     4981 tatagctgat aatctcggct ctttaaaaga aattattttc gcttcatttt attataaaaa
     5041 ggctaggaac cttgatgtta tcaggttcct agcctttatt taagtattac cccacagacc
     5101 cttccatata gatttttaaa ttacacacta gtttaaacta aaacaaacaa ggaaaaactt
     5161 accatttaat caaaaaatta gctcatatga atatattaga atgtaaatta gactggtcaa
     5221 aatctggtca aaaaagagga cgaatacagg tagtcctctt ttttgttatt ttgatattct
     5281 aaatcatttt ttattggatt attcccttgt tcgatataga cccaaattga tctatcaata
     5341 attatcaaac tgagtcatat agagacaaaa atttcaaaaa tgacctgtta aatggtcatc
     5401 gaatatcatt taaaatcaat acgacggagt cagacacgga gtcaaaacaa gcccgctgag
     5461 agtaaaaaat atacagtccc ataggtaaag tgccacagga ctgtatattt ttttatttga
     5521 gattacctga aatgattcca tttaattttt atctcatttt ttcctactag ttagtatcaa
     5581 atactctcct attttctcaa taaactattt tgggttacat ggtatctgaa acagctaaaa
     5641 ccttgtcatt atagcaaaaa atcactttat ctccgtttca tttcagatac atttcactta
     5701 agcggattcc aaaaaaacaa aagaaaaagg aacccctagt tagcctatgc tttttattgg
     5761 ttcctctcaa aacacggtca tataacaacg gggtggggta aatgaatgaa aaaggtggag
     5821 gaaacattat tcaatagctg tcttaaatac ttcgttataa aatgtgaaca aataaaagac
     5881 atctatccct attttctaca cttccacttg actaccatct ttgaatcgta caattttctt
     5941 aactcttctc gtttacctca attcattctc aagtggaatc aactcatcat ctaacatttt
     6001 acgccatgct cgcttcattt cttgtgggta tttaaatggc ttcgcttctg gatgtttggc
     6061 tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa ttggatcttc
     6121 acctggtaga tattgcagat tagaagcatg taattctttc tcagaaacaa accactcatt
     6181 ggaaaagaca tgaatcaaat catcactcgc ttgtgtaaaa tagcgacgtt taatgacaaa
     6241 gatatcttca ttactatcta aagtccccac atcaaatgca tttaaaattt gtcggtaaac
     6301 ttctttgact ccttctgttt tattcaattt agccaaagct ttttcaattt cttctcgtgc
     6361 atctgaacta ttcgcagcat aagttcctag taattgatca atataagttg aatcaatatc
     6421 atacaactta gtggtatttt cactataaaa ttcgatcgtt gaaaaatctt ctccttcttt
     6481 ttctggttct tcctctatta atgaaccctt cacggtctcg tacacgccaa tttgttcttc
     6541 caaaagttga atttgattgt tcaaagttgg agatttcacc atgtcatcat tgtaatcgtc
     6601 ataagtcact aacgattcat aagcacgatt caattcttga aatgctttga cgtactcgat
     6661 acggatttca agtggcgtat gttcatcaat ggattccatg gatgaaacct cttttaactt
     6721 accatatgct tgtttgaatc tttttttgga ttcctgataa cttggataaa tcaaacctga
     6781 ttcttctttt gcttttgaat acagttttgt cgcatcctcg acattcttct ccattgtcgc
     6841 tggcttacga aacgtcacaa tcaatccttt tgtcttatca ggatacgtac gattcgtacg
     6901 tgaaaaagct tggatcaatc ctgcatactc tagattacga tccacaaata gggtttgaat
     6961 cgtaggggcg tcaaaacctg ttaatagacg atccactaca atcgctaaat cgatttgttt
     7021 cccaaattgt ttgaattccg ctctttttcg tgccaaacga ttattgatgt ctccgttata
     7081 acgctcaatg tctgctaatg accaggaagt atcataatac tgattatatt cctcaataat
     7141 tttttgcatt tcttcttgtt gggcacttga atctcggcta ttttcttcta atgaataggt
     7201 aatagcgatt cgtggaaaat caggatcctc catcgttcgt ccttcacgaa tgagctgatt
     7261 gggaaactct tttgttaacc aatcaggctc tttcgtcatc tctttgatgg cccgataata
     7321 ctttttggcc atgtcaatgg aactcgtggt taagattgca gatttcgttg gcctgccatt
     7381 tcgaaaatca aattttgtat aggcattatc tggacggaac attttatgaa tgactttttg
     7441 gatatgttca tctgcttcaa aaacagctgg gtccaaataa ctttcttttt tgacaggttc
     7501 catttgatca atcatacgat tgatctgttc tgatgaatac tctgcatatg tttctacttc
     7561 ttttaatttc cgatagattt tattctctag tgaagtcggc tccacggtgt tttcatgctc
     7621 tacttgaaat cctaagacag agccatcttc tagcgcattc ttaatggtat aggtatgcaa
     7681 gacctctcca tactgatcat gggttgtacg tgccaattgc cctttggctt gtttccgatt
     7741 ctcaggaaaa atcggtgtac ctgtaaagcc aaaccaagtg gatttcggaa acattttttt
     7801 gatttcttcc atattttcag cacttaacgc tcgatgacac tcatctacta caaacacgat
     7861 atgttggccc attaattttt ggaagcgatt ggttcctttt ttctcttctt gtttctttgc
     7921 atatttcaac gccgcatcta gtttttgacg ggttgtaatg attacaacat ttgcattggc
     7981 atctgccaac agggtttcac ttaattcttt ggcacttccc gttccaacga tcaagctatt
     8041 cgctttggcg ttcccggaag aaatcccagt attaaattcc gaagcaaatt ttgtaaactc
     8101 tgtggtggtt tgattatcta aatctttgcg gtccactaac atgatcgttc gatcgatccc
     8161 cgattttcgt gctaaaagct tagtagatac aaaactagtc aacgtctttc ctgaaccagt
     8221 cgcatgccac acataccctg attgatgttt atttgcggca ataaacaagg cttgaatcgc
     8281 atgtacttga taaggatgta acaccatgag ggttttatta tcttgatctt cactgacgat
     8341 tgtataatcc gcaatcaaac gatgagcatc cgggatattt aacacttgtt tacaaaactc
     8401 atataagttt tctacttttt cattgtcttt cgttcgccaa ctaaataaaa acttagggtg
     8461 caagtctttc ggtaaggcat ttgcaaaata ccgagtggtt tgttcattgg aaaccacaaa
     8521 caactgtaag gtcgaaaaga tattgttccg aaacatacct tcttctgcat attttttgat
     8581 ttggttaaat gcctgataaa caccatcttt tgcagttact tgtttcagct cgatttggac
     8641 aatcggtaat ccattgatta ataaggtgac gtcaaaacgt cgatcccgtg cttctatgtt
     8701 acttccacgt tttgcaattt gatggaccac ttcataagta gagattcctc caccaatgtc
     8761 ttgattggaa taaaggataa gtgagacaga acccaattgc gaatcttccc gttcaatcgt
     8821 gatccgagcc atcccatttt caccttttaa ccatttcgca gcatcaaatg gggttttcgt
     8881 tcgcagcagt aattccgttt tgatcgtttc aaattcttta tcggtcaaag gaaaatcatt
     8941 cagttctgct tgattatttg agataatttt ttggcgtaaa ttgacccaaa ggtcttcttc
     9001 tgattttaga tccggacgat acgtccactg attatgccct tctccaagta cacgaatcaa
     9061 ctgttcttcg acttctgctt cctcacggtg actaattttc attcttcttc cctcctagac
     9121 aaacatcttt tgtaagaagc tttttttcgt ttcttttagt aaatctaact tacgttgatg
     9181 aagagcgatc gtatcatcta gctgtttgaa gaaagtaccg attttttgtt gttcatcctt
     9241 tacaggaacc ttaacactga tattctcaat atcagaactg ttataagaag gcaatgcccc
     9301 tacttgggag tatcttggca aatttatagt ttcaaacaat attttaccaa aatagatatc
     9361 ccaagtatta ttaaataaat aagccatggt attgttatct attaagaaag ggcttgtgac
     9421 caaacgtttt ctattaagca ttattgctgc gccaacttta gcaaatataa tcgctggcac
     9481 atcttttatt ggcttccatt ttttacgctc aatttgctca ttagatacat aatgatttgc
     9541 atttctcatt tcatactcat taccaatgtt attcatgtcg gaaactttaa aaaatggagt
     9601 accttcagaa ccaccttgct cggcatcagg aaatccaatt cccgattgag ttttacctaa
     9661 ttctcccaac ttacgctctt cccaatcttc cgtaaaacca ggaaaccgga tttctggcac
     9721 ttttgctccg ttttcaggga acattttttg taagaatcct tttttcgttt ccttcagcaa
     9781 atctaactta cgttgatgaa gagcgatagt gtcgtcgagt tttttgaaga atatgccgat
     9841 tttttgttgt tcttttaaat cattgggaat atagaatact gtatctgaaa cttttttcca
     9901 gtctgaacga ggcatttttg ttccagatgt atcatttgca gccttttgat atctatcagc
     9961 ttgaataaga gaataaataa agtccgagtc gctcttctta gctctaaaaa cccagaaatc
    10021 cccaatcgct acgcctgtaa aagttgcctt gagccaattt tttaaatatg gacgtaactt
    10081 tccatataga atatcatttg gtaaaaattc aatccctttt ctattatcaa atttctggct
    10141 cacatcttta ttcagtctac cttctcctgc cacgatatct tcaaattcaa cttttggaag
    10201 tctactggag tttgtacttt tattaagtct aacaactaaa tcaattaact tacgctcttc
    10261 ccaatcttcc gtaaatcctg ggaaacggat ttctggtcgt gtatcattgc tcatgtttga
    10321 acacctccag cgcaccttgt aaccacattt cgttttcagg tgttgtttgc agggaagaga
    10381 tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc ttgatgtcat
    10441 ttccgacatg aaccatatca attggttctt cttcttcgaa agtatctaca tagcgtggga
    10501 tatttaagtt atagtcattt tctttgattt catcaaaggt tgccaaatga gcgtatttct
    10561 cgacatcttt tctttcagcg taggtttcga ggattttttg aatgttttct tccgatagtt
    10621 tattttgatt tttcccttta acaaattcac gactagcatc aatgaataat acatctcgag
    10681 tttgacgatt cttcttcaac acaatcaccg tcgttggaat cgatgtccca aagaacaagt
    10741 tcgcaggcat accgatcaca gcataaatac tgccatcttc tagtaatttt tgacggatga
    10801 ccccttctgc agcgccacgg aataaaactc cgtgtggtaa gacgatggcc atggtacctg
    10861 tttccttcaa atgatagaac ccatgaagaa gaaacgcaaa atctgctttg gattttggcg
    10921 ctaatttccc ataacgatta aaccgagaat catctaagaa agtcgtatct gctgaccaat
    10981 tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca tctgttggcc
    11041 agtccttatt caacgtatcc ccattgcgga gattcatttc ttcggcgtcg acaccatgta
    11101 aaatcaagtt catttttgct agattgtatg tcgttgtatt tagttcttgc ccatgatatt
    11161 taacgttatc tgggtgtgtt aagtagttac gtacgtttaa catcaatgaa cctgaaccca
    11221 tcgttggatc aaaaacacta aagaatcgtc gttccttttg atcgagcgtt acgatttgcg
    11281 ccatcatatc agaaaccata tgaggtgtgt aaaattctcc ggctttcttc cctgcttctg
    11341 aagcaaattg actgatcaga aactcatacg catccccgat cacgtcccca tcgtgttcta
    11401 aaacatctac atcattcaat ttcttgatga cttcggtaat cgtcacattt cgttgttgct
    11461 catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca ttaaattgat
    11521 tgtaggtaga tgccaattgc acaaaggctt tgtttagatc gttcaattgg aacgttgctt
    11581 gttttgcttg atccgctaag acgttgaaaa gatactctgg tgcaattgca tatcccaaaa
    11641 tatcaacgat tgtggcaatc agatcttctt ttgattcttc atctgaaagt aattcacgat
    11701 acaacattgt ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa
    11761 ccacttgttc caacaatcga tcggataaat atttatagaa aatcagtccc aataaataat
    11821 ttttatattc tgacgcatcc attttactac ggagattatc tgccgcacta aataattttt
    11881 gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt gctatatacc
    11941 ctcttatttt agcatacccc cttttataaa aagcaaaaaa aaaagaaaga aacttattct
    12001 tgctaataaa ctacgcgaaa ggagtctaac gttgtttgat ataacttaga ctgatcgtta
    12061 acttattttt tatatttcta catagttatc tttagaacca tttcagatac atatgaaaca
    12121 attacatatg attttttcat aaaaaaacag tactgtctag tagtataaaa tatacaaatt
    12181 tcttaaaaat tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa
    12241 cgtttaatga aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa
    12301 tccgaaaaaa aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc
    12361 atataacaac ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact
    12421 tttttaaata cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct
    12481 ttttacccat tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca
    12541 ttactgatac ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc
    12601 tctgtatcag tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt
    12661 atagctgtgt atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct
    12721 acttctgtct cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact
    12781 gcttctgtct cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca
    12841 tctttagtgt gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt
    12901 ctttcaatag agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt
    12961 aaaagcattt tgttgtttac atctacataa taatcctttg actctacttg agaccttcct
    13021 atacggaata cagctttact gtctgatatc tctataccag catccttatt attaatctgc
    13081 caatctccaa gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca
    13141 ctggtagagg tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga
    13201 gccttagcac aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta
    13261 cacccttgaa atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat
    13321 ctcttcttga acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg
    13381 cttcactgaa gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg
    13441 ggcaataaaa aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac
    13501 ctttttttcg ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga
    13561 gagcatggtt tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat
    13621 gtttccagcc tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc
    13681 atagcagccg atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt
    13741 ttgaaaaaca atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt
    13801 aaaaaataga cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg
    13861 cttgacgcaa ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt
    13921 tagagcatca gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa
    13981 atcgcctttc tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg
    14041 cttatctaag caaagaatta aactgttgtt taacgtttta attactctca ttagattcac
    14101 cgttctctct aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct
    14161 ttttttattc gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca
    14221 taacgcttat tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa
    14281 ccaaaaacat ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga
    14341 taatcaattc gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca
    14401 tagccatttt cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt
    14461 aatcgtagtc cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt
    14521 tcagctgccc ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg
    14581 gaaacatctg atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc
    14641 gcaataaaat cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga
    14701 tataatccac gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct
    14761 acatctaaga aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct
    14821 ggactggagg tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta
    14881 agattcaact gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta
    14941 atttgaaaga gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca
    15001 ggatgaagga tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca
    15061 tactcagtaa gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac
    15121 caaccgccct gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt
    15181 tcgattccct gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga
    15241 ttggttttat tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct
    15301 tttaagccta attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag
    15361 gcaacttcaa aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt
    15421 ttcccatcct cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa
    15481 aacttctctg gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat
    15541 ttgcatcaat ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt
    15601 ttcactgttg gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc
    15661 gaaatcggcg ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac
    15721 ttcaaagggt tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat
    15781 accttcttta gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc
    15841 ctcaacaggt gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag
    15901 tcccttagaa aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg
    15961 attttcaaca atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc
    16021 aaatccaatt agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat
    16081 tgcgtgaaca atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg
    16141 ggaaacaaag gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc
    16201 accaatcatg gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg
    16261 ctcagtaatt cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt
    16321 gtttttagtt ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag
    16381 cattcctggc ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg
    16441 cgtcattgcc caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa
    16501 tggaattaac caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt
    16561 cagcaacgtt ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac
    16621 cagcatcggc ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc
    16681 tacataggac atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc
    16741 agctaatacc ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat
    16801 atttggatga agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt
    16861 tatggctgcc ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt
    16921 attcaaaatg atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc
    16981 caaaatggct tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt
    17041 agaaatgaca ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt
    17101 tttaattgaa gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc
    17161 aatcaccact tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc
    17221 taaggctttc gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca
    17281 atgggttaag ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac
    17341 ttctctgtaa tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg
    17401 ccccaatcaa cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc
    17461 caataacgtt attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg
    17521 aatttaattt tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc
    17581 aactagggta tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac
    17641 cagtaaaacc attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa
    17701 tgcatataac ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg
    17761 gtgagtaaag gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt
    17821 ttttttggta aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat
    17881 caatagaatg atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt
    17941 atttctaatc tacatgtagt aagtatttca attccaccaa ttatcaataa aaataccaaa
    18001 catatccata ataatttttt ctttttcatt tcttttatat tgttataaca gtgaataaca
    18061 taaaaattcc cgacaatcat tgtaatattt ttttgtcacc tgtattagta tttaatttat
    18121 aggaaactat aaaaactccc tagtgaattt acacctttgg gagttcctgt ttggacggta
    18181 tgcataatac atttaaatca atttaacatt aacacctcag catatggtat ctccacatat
    18241 atacggtctt tctttggata gtactcaata tttatgttga tagcacttcc attgtctaat
    18301 atagctttag cccggtatgt gttatctcca ttttcatgag taatcgtcac atcatcacat
    18361 gatcgattta agttgtactg ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc
    18421 tctaatccag atgtacctgt catttttgaa aaaataatac ctataatagc caagacaaca
    18481 atacctataa tgacaaactt cttgttcata tttactttat tcaacttttt atctcctatc
    18541 tccaaaataa tttgttctta tccctcttat cagtacattg acatccctaa aagtacacca
    18601 aagattgctc cagctacccc tgctatcatc aaaatcgtac ccattttctc atctttttct
    18661 cgatataagt agcccataat aacagcaaca gcaccaaata caacaggaat aaatgctaac
    18721 gatactacca tagaaatcca accaataatc aaatacagct tccctgtctc ttgtccatgt
    18781 acgatttgtt ccttcatttc attaattgtc tgctcagtac ttttctttac tgtgtctgtt
    18841 ccggttgtct catctgtttc ttgtagtgct ccacaaaact ggcaatactt actttcatct
    18901 gttatttctt tgccacattt tctacaatac atgtcttctc ctcctaaaat cattcaattg
    18961 cttattagtt aactgtttac tttattaaat acatcagaaa acagctcatt cattggtaca
    19021 cttaatatct cagaaagcat cttcatttga tagggtttag gaatcgttct ccctgtttgc
    19081 cacgaactta cacattgctg tgtaacattc attttttttg ctaactccgt ttgagaaagt
    19141 cctttttctt ttctgtatcg gctgagatta cttacttttt tcatgattct cccctcctgt
    19201 tttattatat acaacaatat gatgcatttc aactcataaa atataaaaaa cttcaaatac
    19261 cattacaaat acaacaactt gttgtactat actgagatgg gggtgtatta atgaaacaga
    19321 aacaatatgc aaaggtcatt agagaaaagc gaaaagctct cgggtggaca caaaaagaat
    19381 tagctgaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
    19441 agcctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
    19501 ttctagacaa tgttgtcgta gattatgagg aagagttttt ggctctctat cgttctctta
    19561 gcactgaaga tgcagttcga acaattgact atatgaagct attaaaaagg caagaaaatg
    19621 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
    19681 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tacaattggg
    19741 tcttttctct tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgatacaga
    19801 ggaactatcc cagctcctga taatatcaat tcttttattg tttcatctat ccctgcccct
    19861 ttcgctaaat atattaaaaa actactactt gctttcacat tgtatagttt cttaatatcc
    19921 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttttattaaa
    19981 gcatttttca cgctgttatc cccaacatta cgtaggcgtt cacaaacagt gttaataagt
    20041 tctttctctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
    20101 tcatctaaaa tcaattgttt ttccttgctg agttctgatc gggaattttg aggaaatact
    20161 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
    20221 gtaaaatgaa ttttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
    20281 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgattaaa gtttcctaac
    20341 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
    20401 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
    20461 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
    20521 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
    20581 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
    20641 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
    20701 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
    20761 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
    20821 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
    20881 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
    20941 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
    21001 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
    21061 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
    21121 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
    21181 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
    21241 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
    21301 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
    21361 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
    21421 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
    21481 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
    21541 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
    21601 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
    21661 ttctctaata atttttcttg atctacataa attggaatat taatcattga tgtttccatt
    21721 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
    21781 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
    21841 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
    21901 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
    21961 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
    22021 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
    22081 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
    22141 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
    22201 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacagct
    22261 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
    22321 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
    22381 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
    22441 aacggtcaat taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca
    22501 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
    22561 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
    22621 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
    22681 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
    22741 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
    22801 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
    22861 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
    22921 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
    22981 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
    23041 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg
    23101 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
    23161 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
    23221 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
    23281 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
    23341 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
    23401 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
    23461 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
    23521 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
    23581 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
    23641 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
    23701 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
    23761 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
    23821 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
    23881 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
    23941 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
    24001 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
    24061 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
    24121 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
    24181 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
    24241 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
    24301 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata
    24361 actgatcaat cgattgagtt cgactgcata ttccattgga agttctttgg tgaatggttc
    24421 aacaaagccc atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag
    24481 atagtagagt tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg
    24541 gctgttgtgg atctcattga atgggatcgt atcggatttc gaccattcgt ccatgatgat
    24601 cgtgtcacat tcgatatgag agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc
    24661 tcgatagttt acttctccgc cgtcttttga aattgattta gagacgatag agctagatgt
    24721 atttggtgca ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa
    24781 ggcgatggag agcatggtgc ctcgtgcacc ttttccatct aagtaaacac ttgggtattt
    24841 catcgttgtt tttgcaccta ggtttccgtc gatccattca acggttgcgc cttcgtatgc
    24901 ttttgctcgt ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta
    24961 acggcaatac gcatcttttc ttgtaaagat ttctacgatg gcggcatgca ggctgttact
    25021 tgaataagta ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacaat
    25081 gatcagcgta cgttcgaatt gccccgtgtt ttccgcattg atccggaagt acgtttgtaa
    25141 tggaacatcg actttgacgc cttttggcac gtaaataaag gtcccacctg accatacggc
    25201 tgaattcagc gctgctaatt tgttgtcggt tggcggtaca agttttgaaa agtactcttt
    25261 gaataattca gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt
    25321 ttcgaattca tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc
    25381 ggctagataa gctcgttcag cttctggtat accaatcttt tcaaatgtat ctttgatttt
    25441 ttctggtacg tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat
    25501 ggcatcgaaa tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa
    25561 tgtttctaga gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga
    25621 catctcacgg atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc
    25681 atcgtgaaac ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt
    25741 ccttgtggta atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt
    25801 tccaggcaag tgtcgcacat ttgatccgag cgggaaattt agctacacca ccaagcatag
    25861 cagcatcgcc taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt
    25921 ctacagttaa atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg
    25981 aggcacttgc cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc
    26041 cgtcttttac atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga
    26101 tcttgcttgt cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga
    26161 tcacttgacg gtataaattg tctaatttag atagtcccat gttggaaaaa ctcctttgtt
    26221 gccatgattg cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc
    26281 gcgcgagcag tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct
    26341 gctctgacgg ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca
    26401 tccaaattga atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc
    26461 aatccatcaa ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga
    26521 tggatattgt ccataccgag tttttgcaaa taatcgattg catggcctaa tgcgatagcg
    26581 ccggcgatat ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg
    26641 tataattcga caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac
    26701 cattttcgtt taccatataa aacaccaata cctgttggtc cgcacatttt gtgtccgctg
    26761 aaagcaaaga aatcacagtc tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga
    26821 gcaccatcta ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca
    26881 ggattgaaga cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc
    26941 gtaatctttt tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat
    27001 ttcaagacag cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt
    27061 tccatgtaag agagcactat ctcgtctcct gctttgatat agcggtcgcc gaaggttctg
    27121 gctacccaat tcaagcttgt cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt
    27181 gcatggatga acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct
    27241 gctaacgtat ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtacgctaat
    27301 gcctggagta cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt
    27361 tcatcattga cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc
    27421 atccattcaa cttcccttcg attacttcga ctaattcttt ccgaacttgt tctacaggga
    27481 ttgctgttaa gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat
    27541 gcaatcctct actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag
    27601 catgtccagc agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg
    27661 ctttatcaga aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt
    27721 tcaagatgtg gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt
    27781 gcaaaatatg cccgatcgta tgaggggctt tattggttac tcgtgtatcg attccttgga
    27841 tttgtctgcc cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta
    27901 aatctgagtc aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga
    27961 cagaagcatc tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttctccta
    28021 attgatctac ggctgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga
    28081 tattacctga tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa
    28141 attcactatg ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc
    28201 catcttgaat gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca
    28261 ggaaaatacc gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt
    28321 ttactgcttt tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga
    28381 acaagtcggt aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg
    28441 tccatgaacc ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg
    28501 tgcctgtttc actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt
    28561 tcacccgatc gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga
    28621 aggttctcat ccaatcaggt tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat
    28681 aatcgagcca gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt
    28741 cgattcctaa ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt
    28801 cagcattccc tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga
    28861 tatagttcaa taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca
    28921 tttcatttac tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt
    28981 cgtctaaaat tgcaaatgta ggttctaaca tcaacaattg caggatctca ttccgtttct
    29041 tttctccgcc agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta
    29101 gtaaagccat tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt
    29161 catcacgttt cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct
    29221 cacttggata ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt
    29281 ctaaaacatt tttcccgtca aacaaaattt cgccttctgt tacttcatag tttggattgc
    29341 ccatgatagc ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga
    29401 tttctccggt tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca
    29461 catgtagatt ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc
    29521 tcactcattt tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata
    29581 tcttttattt attattaaac aattttgatt tctaaattag aataggtata aaaaagatga
    29641 gaaataagcc attacagact tatttctcat cttttttgtt ccatacctta ttttgcatca
    29701 gcctttactg gtttaacaga gccgccccat ttgtctgtga tccattcttg gacttctttg
    29761 cggtgaagca cttcaatcag ttttttgatt ttgtcactgt cttcatcacc tttacgagtc
    29821 gcaataatat tcacgtaagg agagttgtcc ttttcaagca atacggcatc ttctaatgga
    29881 ttcaaccctg cgccgtaagc gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt
    29941 tggtacgtag aagctaaaag tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg
    30001 ttttcttcaa tatcatcgaa tgttgctgtt tccagatcta ctccatcttt gactttgact
    30061 aagccagcat cttgtagaat tgtgatgatg cgtccccagt ctgattcaga atttgaagta
    30121 atgattgttg caccatcttt taattcggaa acatctttga cttttttaga gtacaagccc
    30181 attggttcga tgtggacagc accggcattg acaaagtcat atcctttttc tttgatttct
    30241 ttgttcaaat aaggaatatg ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat
    30301 tggttcggta atacatagtc atcgaatttg acgatttcaa gatcgattcc ttcatctttc
    30361 aataatggtt tcacatgttc caaaatctct gcgtgcggtg tcggtgaagc gccgactttc
    30421 aatgtcgtgt cgtctgcttt atttgctgaa tctttgctgt cgctgttgtt accgcatgct
    30481 gctaatccta cagtcagcaa aagcgctgct gcaaatccaa aaattttctt tttcatatcc
    30541 tacatccccc tatatcctat tatcttttgt ctgttttctt agttaagaaa tcaccgattc
    30601 cttgaacgat aaatacgatt agtaaaataa ttaaagtcgc aacgaatgtc actgttagat
    30661 taccacgctg gtagccttcc tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc
    30721 cggccatcgc agtaaatccg atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg
    30781 cgggaatact ttcaggtatc aagaccttcc agatgatttc ccagtaactt gctcccattg
    30841 catctgctgc ttccagtaca ccagggctga cttcacgaaa agcaatctct actagacggg
    30901 cataaaatgg tgctgcggat aaaacaagag ctggaatggc tgcttgcgca cctagcatag
    30961 tgcctacgat tgctttagta aatggaatga tcaaaaccat caggatcata aatggcgttg
    31021 accggaaaat attactgatg atcgatacga ttccataaat gattcgagca tacggtttgt
    31081 ttttgcgtcc aatagagtaa aggatcaatc ccaaaactaa tccgatgatc gtcactaaga
    31141 ccattgagat taatgtcata tatagtgtat caaggatagc ctgattcatc gtttccgggt
    31201 tgatttgttt aaaatctaga tatgtttctg aaaagctttt atccacgatg gatcacctca
    31261 attcctactc cttgttcttt gatgaacact tctgcttgat ctaattcttt ggtttcacca
    31321 gtcatcaaga cagtcaatga accaaaggaa ccttctttca cttgtttgat ctttccatac
    31381 acaacgttga catcgatgtt atagcgtcga atcgcttggg aaatgactgg ctcgttggca
    31441 tttctttcgc taaaggtcaa agttgccaaa cggccgcttg gattttcttt gatgagttca
    31501 gctaatagtt ctgtcgaatc agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt
    31561 acttcttttt tcggttgtct gaagacagtc aatacgtctc cttcttctac gactttcccc
    31621 aactccatca cagcgacttt gttgcagatt ttgcggatca cattcatttc atgtgtgatc
    31681 aagacgatgg tcaaattcaa tttcttattg atcgctaaca ataagtctaa tacttcttct
    31741 gttgtttgcg ggtccaatgc gctagtagcc tcatcacaaa gcaggatctc aggatcattt
    31801 gccaatgctc gtgcgatacc gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat
    31861 gcttgtccgc gtccttctaa cccgactaaa cgcaacagtt cttctgcccg ttctttacgt
    31921 ttgctctttg gtacgccagc taattccagc ggcagctgga tattttcaag tacagttctt
    31981 gaccataaca ggttgaaatg ctggaagatc ataccgattt tctttcggaa tgtacgtaat
    32041 tctttgtttt ttaattttgt aatatcttga tcgtttacga tcacttcgcc gctcgtggga
    32101 agctcaagtc cattcaggag acggactaat gtactttttc ctgcgccaga atagccgacg
    32161 attccataaa tatccccttt ttcaacgttt agtgaaacgt cattgacggc ttggatgttt
    32221 ccgtttttcc cattgaatgt ctttttgaca ttcgttagtt ggattaatga catgccattc
    32281 acatctttct cttatgattt ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa
    32341 ggacgaaagc ttgaacttcc gtgttaccac cttgatttac cgttacttcg caataacagc
    32401 ctcttccagt acttggactt agcttctttc cgtatactgt cgcgctgtaa cgggcactcc
    32461 cgtagtaagt ttgcttacgc tcgcttactc tgctctaaga ccatcttcca tctcattttc
    32521 gttacccatt ttcagcaaac agggctctct tcaaacgtcc gtgacatgta ctcttctttt
    32581 caaagctttc tcgtttcata taggattgct tatgattata gcaactggaa gaatcagtgt
    32641 caactttttt caagtgattt tctgatttta taattcgtca aattcttttg gatcaacatc
    32701 tttgaaactt aagatccacg cattcatttc atctgaatca ttcaagatat tgatatcttc
    32761 accgatttta tcattaacag aagaaactgt acccgttagc gggctggcaa attcattgac
    32821 cgctttttcc gcttcaactt cgatcagagg ttcatctttt tttagcgatt gtcctgcttt
    32881 tggcagattg gcaaacgtga tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac
    32941 acagtattct tttccattaa ataagatcca aaggttgtcc tttttcttca aatgcttgcc
    33001 tgccatttta tatcagttct ccttcataaa ttttttcatt gaatccaatt gcaacaagct
    33061 ggtcctctcg taagatcagt ggtcgcttga tcagcatacc atctgtcgat aagacttggc
    33121 tcgcttttga agccgtaaac tcgcctactt gatctttcag tccaagttcc cgatatttca
    33181 tcccgcttgt gttgaacagg cgacggattg gtacttgact cgtttctagc caagtctcaa
    33241 tcgttgaggc cttcggtact tctttgatca tatcaacggt ttgataacta acgccatgct
    33301 ggtctaacca cgctttggct tttttgcatg ttgaacattt cgggtaccag taaaaggtgt
    33361 acaattttct tacctccttg aagatcttac aggtgcttca ttttggtatc tcatcgataa
    33421 tatcaacccg ataccgatca tgttccctaa aatcgaggat cctccctgac taatgaaagg
    33481 caatgggatc cctgttaaag gaagtaatcc gatatttgct ccaatattct caaagacatg
    33541 gaacagcagc atcatgatca aaccgcttgc gatataggcg taaaattcat tattcgtatc
    33601 gaaacacacc cgaatcatac gatagattaa aataaaatac aagaaaatga cgaatgcacc
    33661 accgataaag ccgaagtttt caccgataac ggtaaaaatc atatcggact cacgaacagg
    33721 cacatagata ttgcttacgt tgaatccttt accaaacaag ccaccggtac caattgccaa
    33781 aagtccttgg gccggctgat aactcattcc tgacgtatca tggaaagggt ccagccatga
    33841 atcaatacgt gcaaactgat aagatttgaa tccaacttta taaagcaagt ctcttccgcc
    33901 ttcagtcgta accaagaaga tcgttcctgc tccaacgact actgccaaga ttactacagg
    33961 tgcgatgatg cgccaagaga ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa
    34021 gacaagcatc gttccaaagt catcttgggc catgatcaat gctaaaaccg gtattgtcac
    34081 gatcaagagc ttcccaatca gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt
    34141 attatgttta gttacgatca gtgccatcat caagatgtac gcaatcttca tcaactctgc
    34201 tggctggaaa gtaaacgcac cgaatcgaaa ccagttttta gaccctgttg aagcagctaa
    34261 attctgatca tagaatttga gcaagagtcc cattaccact aaccccaaac cataaaggta
    34321 aggagtcagt ttccagatcc atttggaatt caaatgcatg atgacaacga ttgctgccgc
    34381 tccaactaaa taccagacaa cttgcatccc tacgcctttc aagactgctg gattgttcgg
    34441 atcatggcta agtgctacat ataatgagag cagaccaatc aaacataaca gaaagactgg
    34501 taaaatcacg ccataatcaa tacgattatc gttcgaagct tttaattttt tttccatgtt
    34561 tcatcccttt tcttaaaaac catactgctg attattatag cggtccagtt agataatgga
    34621 aagtctttcc atagacattt gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa
    34681 atgcagtaca aaagcctact gcttatcttt agtgtatcat agaacttttt ttgatacatg
    34741 caattatcta ccagctgaga ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc
    34801 gaaggatctc ttcggcaatc tctagcagct gatcagaaac cagattggtt cgttggatga
    34861 aataaaaata tcgatggaaa ttcaacggag tcattggcac attttctgga acagagcgct
    34921 tagacaaaat cgagcgaccg attccttcct tcagcaacgc aacaattaca tcgttacttt
    34981 tcacgatcat cttcttatct tggatattca tctcttcaaa ataacgctgc gtgtaatgaa
    35041 aaacaccaga gtttgcttct cttacaagcc acaatttgct ttttgggtcc ccagcgatga
    35101 ctagctggtc ttccatcaaa gagtatcttt gtacgccgga agtctctaat ggcttttcga
    35161 taaaaccaaa atcggattca tgtttcgcta cttgttctaa tgattcatga gaattcgtga
    35221 tttcgatctc aaaatgcact ttaggaaacc ttttgatcag atgtttcatc aactgaggca
    35281 aaagatagac agcaaaggtg tgggaagcaa cgatccgaca tacttcttct tcattttgtt
    35341 cattgagcaa tttttgctga gtttcttccc attcttctaa caaaaccagt gtccgttcat
    35401 ataataagtc ggcttgctta gttggaagca tctcttttct tccgttacgt tgaaaaagat
    35461 cgatattcaa ttcccgctcc aactgtttga tttgattcga gactgctggt tgagataaaa
    35521 acagtacttc tgctgctctg gaaaaatttc ttgtttcata tactgctcga aaagtcgtta
    35581 ataacttaaa catggcacca cttccataac tttttatgat aattactata aaaactattt
    35641 gatttaaata tacaaaaaac catctataat agaaatgtac aacaaaacag ttgctgatcc
    35701 aactagtaac ttagatgaag acaatcagct gtctgcttgc tcgtaatcat acattcctat
    35761 tcattttaca atgtattcgt tcatcttggt actaaaatct attaaataaa aaggagaata
    35821 ttatatatga agaacttttt gaaaattatt ccgatcccga tctgcgggtt gatccttggg
    35881 ttagcttccc ttggaaatct gtttaaagac tatcatcatg ttgctttagg caatctaact
    35941 ggcggtatct ctatgattct tatgatattc atttttgtta aattgtttat cttattcgaa
    36001 cacacgaaac aaacgttgca tgatccaatc atcgcctccg tttcaccaac ttttacgatg
    36061 tcactcatgg ttatttgtac ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa
    36121 ttcatttggc tgtgcgctgt tatcttccaa gtgatccttg tcttttattt caattatcac
    36181 catgttatca aagctgatct ttcaattgaa gcaatttatc caagctggtt tatcatttac
    36241 gttggatttg gggtcatcac tgtaactgcc gggaacttct tcccacttat tggaaaaatt
    36301 ttcttttgga tttcattgat ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc
    36361 tttttcgtaa gaaatatggc tcatccgaca cgtccgctga ttacgatcgt tgcagcacct
    36421 ggttctcttt gcctaacagg ttatcttaaa aactttgctg aacctaattt ttatcttgtc
    36481 gtagcattgt tcgctttatc tcaagtattg tatttcgttg tgctgtctat gcttccacgt
    36541 ttgttcaagc ttcgttttta tccaagctat gcagccttta cattcccgct tgttatctct
    36601 gcaacagcta tatttactac tgttcattac ttcaacagtt taggtatcta tagtagcgta
    36661 ttggatttct taaaagttgc ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt
    36721 gctcattacg tacgtttctt aattaaagaa catagcaaac atggaaaatt tgtttctaaa
    36781 aaaatcgctt aataatcgat tcaataggtt gtgaaaaagc tgttctgcat caagtaataa
    36841 gaacggccaa acaaaaacag ctgttcatgt ttttgtactg gttgggctta ttaccgcaga
    36901 tgcaagcttt tgaacaccgt ttaccaagag gtcgtaa
//
LOCUS       DACCDF010000021        36471 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCDF010000021 DACCDF010000000
VERSION     DACCDF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.021"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(92..730)
                     /locus_tag="IVK51_001027"
     CDS             complement(92..730)
                     /locus_tag="IVK51_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6430636.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(853..1485)
                     /locus_tag="IVK51_001028"
     CDS             complement(853..1485)
                     /locus_tag="IVK51_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6430637.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1836..2135
                     /locus_tag="IVK51_001029"
     CDS             1836..2135
                     /locus_tag="IVK51_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP6430638.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            2630..4795
                     /gene="acm"
                     /locus_tag="IVK51_001030"
     CDS             2630..4795
                     /gene="acm"
                     /locus_tag="IVK51_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP6430639.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(5067..5552)
                     /locus_tag="IVK51_001031"
     CDS             complement(5067..5552)
                     /locus_tag="IVK51_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP6430640.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            6032..6673
                     /locus_tag="IVK51_001032"
     CDS             6032..6673
                     /locus_tag="IVK51_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6430641.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            6692..7327
                     /locus_tag="IVK51_001033"
     CDS             6692..7327
                     /locus_tag="IVK51_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6430642.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            7345..8160
                     /locus_tag="IVK51_001034"
     CDS             7345..8160
                     /locus_tag="IVK51_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6430643.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            8479..9915
                     /locus_tag="IVK51_001035"
     CDS             8479..9915
                     /locus_tag="IVK51_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6430644.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            10104..12551
                     /locus_tag="IVK51_001036"
     CDS             10104..12551
                     /locus_tag="IVK51_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6430645.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            12781..13848
                     /gene="rlmN"
                     /locus_tag="IVK51_001037"
     CDS             12781..13848
                     /gene="rlmN"
                     /locus_tag="IVK51_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6430646.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(14041..15144)
                     /locus_tag="IVK51_001038"
     CDS             complement(14041..15144)
                     /locus_tag="IVK51_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6430647.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            15697..16245
                     /gene="aac(6')"
                     /locus_tag="IVK51_001039"
     CDS             15697..16245
                     /gene="aac(6')"
                     /locus_tag="IVK51_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6430648.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            16791..18452
                     /locus_tag="IVK51_001040"
     CDS             16791..18452
                     /locus_tag="IVK51_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6430649.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(18522..18869)
                     /locus_tag="IVK51_001041"
     CDS             complement(18522..18869)
                     /locus_tag="IVK51_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP6430650.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            18953..19894
                     /locus_tag="IVK51_001042"
     CDS             18953..19894
                     /locus_tag="IVK51_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430651.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            19895..20938
                     /locus_tag="IVK51_001043"
     CDS             19895..20938
                     /locus_tag="IVK51_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430652.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            20944..22014
                     /locus_tag="IVK51_001044"
     CDS             20944..22014
                     /locus_tag="IVK51_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430653.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            22004..22957
                     /locus_tag="IVK51_001045"
     CDS             22004..22957
                     /locus_tag="IVK51_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430654.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            22969..23979
                     /locus_tag="IVK51_001046"
     CDS             22969..23979
                     /locus_tag="IVK51_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6430655.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(24264..25085)
                     /locus_tag="IVK51_001047"
     CDS             complement(24264..25085)
                     /locus_tag="IVK51_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP6430656.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(25182..25460)
                     /locus_tag="IVK51_001048"
     CDS             complement(25182..25460)
                     /locus_tag="IVK51_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430657.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            25728..27737
                     /gene="metG"
                     /locus_tag="IVK51_001049"
     CDS             25728..27737
                     /gene="metG"
                     /locus_tag="IVK51_001049"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP6430658.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(28216..28803)
                     /locus_tag="IVK51_001050"
     CDS             complement(28216..28803)
                     /locus_tag="IVK51_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430659.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            28978..30300
                     /locus_tag="IVK51_001051"
     CDS             28978..30300
                     /locus_tag="IVK51_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6430660.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            30455..31231
                     /locus_tag="IVK51_001052"
     CDS             30455..31231
                     /locus_tag="IVK51_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6430661.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            31224..31793
                     /gene="rnmV"
                     /locus_tag="IVK51_001053"
     CDS             31224..31793
                     /gene="rnmV"
                     /locus_tag="IVK51_001053"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP6430662.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            31786..32670
                     /gene="rsmA"
                     /locus_tag="IVK51_001054"
     CDS             31786..32670
                     /gene="rsmA"
                     /locus_tag="IVK51_001054"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP6430663.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            33121..33750
                     /locus_tag="IVK51_001055"
     CDS             33121..33750
                     /locus_tag="IVK51_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430664.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(33874..34983)
                     /gene="ugpC"
                     /locus_tag="IVK51_001056"
     CDS             complement(33874..34983)
                     /gene="ugpC"
                     /locus_tag="IVK51_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP6430665.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            35192..35614
                     /gene="mgsA"
                     /locus_tag="IVK51_001057"
     CDS             35192..35614
                     /gene="mgsA"
                     /locus_tag="IVK51_001057"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP6430666.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            35937..>36471
                     /locus_tag="IVK51_001058"
     CDS             35937..>36471
                     /locus_tag="IVK51_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6430667.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
BASE COUNT        12215 a         6251 c         7430 g        10575 t
ORIGIN      
        1 atttttaatc cattgataca tctgtctttt tgtttaaagg aaataaagta tgctttctta
       61 gcaatagagc atgtattatt tctataaaaa tttaggagaa taaaacgact agcaatatag
      121 ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat gatcgagttg cttttaatgg
      181 cagcttggtt gaataacaat cttgccaccc cttgtatcaa aaggatgtgt gctacagtgt
      241 gtaaaatatt tccattatca taagtactgt aatggataaa tccaaagatg attgctgaca
      301 agaaactagc gacaggaatg ctgaacttac tagaaaaaat agcgagaaaa tacactgaga
      361 acaattcttc tccaagtatc atgataggca aaataataaa taataacggg ctgaaagcct
      421 catttattgg attgcctgtt aaaccccatt tcaaaatttg ttgcaaaaat accgagactg
      481 cggcactgat tactacattg ataaaaaaat atttggctat atttttgaca ggagcgacag
      541 gtttcgagaa catttttttc gtatcttttt taccaaaaac aagtgtaaat gctataaatc
      601 cagtgatagt taaagaaaaa atgctaataa tggatggcgc aaatagcaac cctaacaaac
      661 cgagtacaac gacaatagtg cccaatatcc cttttttaag ttctattttt tttcctaaaa
      721 tgttattcat gataataact cctactattc agtttttgaa gatagagtca gtatacatga
      781 agagttaaaa taggcaatgt ttttttatgg atatttgtat tgctaaaaat ttatcttata
      841 tattcatttt gattatacta cttttatttg taaaaatttt ctagcaaaac caagtgcttg
      901 caactgatta agcgtacaat tttttagatg ttccgagcgt acttcaatca tttcaaagga
      961 gttgctactt aaatcgatat tttttaaatc agtatcaaaa aagctagcat tttctaaatc
     1021 attagtagaa aacattattt tagtgaggga acagtctaca aaatctgcgc ttgataagac
     1081 agagtgctca aaggtacttt cttttatgtt tgaaccagat aaatttacat agtccatttt
     1141 acaattctcg aataacgtgt tcccaatata actttccatc aatttagtac ctgttaattt
     1201 acaattgaca aatttcacgc gatgcaacat gctttttgtc aatatacagc ctgaaaaatc
     1261 acagttttcg aatatacagt cggtaatctc cattctttga aaatcattgg aagcaaaaaa
     1321 tgcatacttg aaaatagatt tgtaaataat tggatggcag attatttggt tttcgatgac
     1381 ctctttttgt gaaaggatat tatacagttc aaaatcttcg ttttcataga tatgaaaaaa
     1441 ggatccttta ttcaagcttg cttcattaac tttaggaaaa atcatattta attacctcca
     1501 ttttcataaa gtcatattaa caaaagctaa accaacttac attagcagaa aatgagatag
     1561 gttttcgcct ttttcgataa cgatagtgtc gctatctctt gaaacgttct tagaattgcc
     1621 ctgagagaca caaattaggc gtaattctaa taaaaatctt agaataaaaa tttttactga
     1681 tttaatttag aatgttttta aaataaaaat cgttgccata acagtgtatt gaaaggtttt
     1741 aacaatccag taatgattta aaaaaatcta gatgtagcgt atactttgtt tatagaatgt
     1801 agtgggaaga aatatatttt tgggaggaaa tcgtgatgtc taaaagtgaa gaaatagaaa
     1861 acatggtact aaaattatat gatctcgttt taaatccaga gatcaaagaa aaggaacgta
     1921 tacttttgat agacgcaaag acaggcttag aaaaaggtca gtattatcct aaagtcataa
     1981 ataatttgga acgtagttta cgtccgttag caatcagggg agaattgtct aaaccagtat
     2041 caccgtttta tatggagata tcaacaatag gaaaatttga aaaagaatta ggtcgaggaa
     2101 tggcttcggc accgattact tttgggcatt tataaaaacg agttattcat ctcaatgaat
     2161 taaaaagtat agtcaatctc ataatgcagg ctaggttcat ttatttgaac agagtctgta
     2221 tttttttatt ctaaataaag aaacagatgt ttctttatat tgtattttat cctttaatta
     2281 acacaaaaac agctagtata aactaataat gttaattaaa agtgtatttt atgaaactat
     2341 cgttatttat taatgttgca tttttgtaag ttaaaaattg gaaatataga ttgaaaaaat
     2401 gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa taatttacat taggaaaaac
     2461 cttgtaaaaa cagttttttg gcatatttat tttttataaa tatttgtaaa ataactatca
     2521 attttttagt taaaagattg gaaatgagtg ggggaggaat tgaagaaagg atttttgaga
     2581 gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa tgaaaaagtg
     2641 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
     2701 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
     2761 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
     2821 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
     2881 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
     2941 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
     3001 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
     3061 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
     3121 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
     3181 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
     3241 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
     3301 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
     3361 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
     3421 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
     3481 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
     3541 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
     3601 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     3661 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     3721 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     3781 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     3841 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     3901 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     3961 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     4021 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     4081 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     4141 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     4201 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     4261 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     4321 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     4381 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     4441 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     4501 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     4561 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     4621 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     4681 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     4741 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     4801 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc
     4861 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
     4921 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
     4981 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
     5041 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
     5101 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
     5161 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
     5221 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
     5281 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
     5341 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
     5401 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
     5461 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
     5521 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
     5581 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
     5641 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
     5701 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
     5761 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
     5821 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     5881 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     5941 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     6001 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     6061 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     6121 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     6181 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     6241 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     6301 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     6361 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     6421 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     6481 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     6541 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     6601 ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa
     6661 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     6721 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     6781 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     6841 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     6901 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     6961 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     7021 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     7081 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     7141 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     7201 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     7261 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     7321 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     7381 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     7441 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     7501 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     7561 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     7621 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     7681 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     7741 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     7801 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     7861 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     7921 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     7981 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     8041 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     8101 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     8161 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     8221 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     8281 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     8341 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     8401 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     8461 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     8521 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     8581 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     8641 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     8701 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     8761 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     8821 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     8881 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     8941 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     9001 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     9061 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     9121 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     9181 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     9241 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     9301 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     9361 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     9421 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     9481 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     9541 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     9601 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     9661 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     9721 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     9781 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     9841 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     9901 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     9961 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
    10021 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
    10081 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
    10141 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
    10201 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
    10261 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
    10321 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
    10381 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
    10441 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
    10501 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
    10561 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
    10621 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
    10681 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
    10741 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
    10801 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
    10861 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
    10921 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
    10981 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
    11041 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
    11101 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
    11161 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
    11221 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
    11281 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
    11341 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
    11401 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
    11461 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
    11521 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
    11581 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
    11641 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
    11701 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
    11761 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
    11821 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
    11881 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
    11941 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
    12001 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
    12061 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
    12121 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
    12181 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
    12241 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
    12301 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
    12361 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
    12421 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
    12481 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
    12541 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
    12601 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
    12661 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
    12721 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
    12781 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
    12841 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
    12901 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
    12961 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
    13021 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
    13081 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
    13141 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
    13201 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
    13261 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
    13321 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
    13381 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
    13441 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
    13501 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
    13561 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
    13621 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
    13681 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
    13741 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
    13801 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
    13861 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
    13921 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
    13981 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
    14041 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
    14101 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
    14161 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
    14221 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
    14281 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
    14341 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
    14401 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
    14461 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
    14521 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
    14581 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
    14641 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
    14701 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
    14761 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
    14821 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    14881 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    14941 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    15001 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    15061 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    15121 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    15181 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    15241 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    15301 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    15361 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
    15421 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
    15481 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
    15541 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
    15601 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
    15661 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
    15721 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
    15781 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
    15841 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
    15901 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
    15961 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
    16021 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
    16081 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
    16141 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
    16201 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
    16261 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt
    16321 tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg
    16381 tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt
    16441 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
    16501 gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat
    16561 ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc
    16621 ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt
    16681 atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa
    16741 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
    16801 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
    16861 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
    16921 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
    16981 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
    17041 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
    17101 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
    17161 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
    17221 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
    17281 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
    17341 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
    17401 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
    17461 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
    17521 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
    17581 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
    17641 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
    17701 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
    17761 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
    17821 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
    17881 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
    17941 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
    18001 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
    18061 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
    18121 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
    18181 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
    18241 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
    18301 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
    18361 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
    18421 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
    18481 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
    18541 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
    18601 ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt
    18661 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
    18721 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
    18781 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
    18841 actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc
    18901 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
    18961 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
    19021 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
    19081 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
    19141 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
    19201 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
    19261 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
    19321 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
    19381 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
    19441 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
    19501 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
    19561 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
    19621 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
    19681 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
    19741 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
    19801 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
    19861 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
    19921 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
    19981 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
    20041 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
    20101 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
    20161 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
    20221 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
    20281 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
    20341 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
    20401 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
    20461 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
    20521 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
    20581 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
    20641 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
    20701 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
    20761 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
    20821 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
    20881 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
    20941 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
    21001 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
    21061 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
    21121 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
    21181 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
    21241 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
    21301 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
    21361 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
    21421 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
    21481 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
    21541 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
    21601 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
    21661 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
    21721 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
    21781 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
    21841 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
    21901 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
    21961 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
    22021 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
    22081 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
    22141 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
    22201 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
    22261 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
    22321 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
    22381 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
    22441 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
    22501 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
    22561 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
    22621 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
    22681 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
    22741 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
    22801 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
    22861 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
    22921 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
    22981 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
    23041 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
    23101 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
    23161 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
    23221 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
    23281 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
    23341 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
    23401 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
    23461 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
    23521 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
    23581 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
    23641 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
    23701 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
    23761 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
    23821 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
    23881 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
    23941 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
    24001 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
    24061 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
    24121 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
    24181 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
    24241 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
    24301 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
    24361 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
    24421 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
    24481 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
    24541 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
    24601 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
    24661 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
    24721 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
    24781 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
    24841 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
    24901 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
    24961 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
    25021 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
    25081 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
    25141 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
    25201 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
    25261 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
    25321 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
    25381 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
    25441 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
    25501 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
    25561 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
    25621 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
    25681 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
    25741 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
    25801 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
    25861 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
    25921 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
    25981 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
    26041 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
    26101 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
    26161 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
    26221 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
    26281 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
    26341 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
    26401 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
    26461 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
    26521 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
    26581 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
    26641 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
    26701 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
    26761 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    26821 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    26881 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    26941 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    27001 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    27061 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    27121 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    27181 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    27241 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    27301 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    27361 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    27421 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    27481 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    27541 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    27601 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    27661 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    27721 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    27781 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    27841 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    27901 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    27961 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    28021 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    28081 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    28141 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    28201 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    28261 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    28321 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    28381 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    28441 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    28501 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    28561 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    28621 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    28681 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    28741 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    28801 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    28861 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    28921 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    28981 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    29041 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    29101 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    29161 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    29221 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    29281 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    29341 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    29401 ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata
    29461 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    29521 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    29581 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    29641 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    29701 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    29761 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    29821 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    29881 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    29941 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    30001 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    30061 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    30121 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    30181 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    30241 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    30301 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    30361 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    30421 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    30481 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    30541 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    30601 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    30661 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    30721 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    30781 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    30841 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    30901 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    30961 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    31021 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    31081 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    31141 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    31201 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    31261 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    31321 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    31381 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    31441 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    31501 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    31561 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    31621 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    31681 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    31741 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    31801 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    31861 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    31921 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    31981 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    32041 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    32101 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    32161 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    32221 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    32281 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    32341 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    32401 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    32461 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    32521 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    32581 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    32641 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    32701 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    32761 ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    32821 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    32881 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    32941 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    33001 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    33061 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    33121 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    33181 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    33241 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    33301 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    33361 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    33421 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    33481 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    33541 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    33601 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    33661 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    33721 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    33781 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    33841 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    33901 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    33961 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    34021 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    34081 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    34141 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    34201 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    34261 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    34321 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    34381 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    34441 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    34501 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    34561 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    34621 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    34681 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    34741 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    34801 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    34861 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    34921 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    34981 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    35041 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    35101 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    35161 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    35221 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    35281 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    35341 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    35401 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    35461 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    35521 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    35581 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    35641 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    35701 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    35761 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    35821 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    35881 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    35941 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    36001 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    36061 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    36121 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    36181 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    36241 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    36301 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    36361 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    36421 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa t
//
LOCUS       DACCDF010000022        35882 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCDF010000022 DACCDF010000000
VERSION     DACCDF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.022"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1152
                     /locus_tag="IVK51_001059"
     CDS             <1..1152
                     /locus_tag="IVK51_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430668.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK51_001060"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IVK51_001060"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP6430669.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IVK51_001061"
     CDS             2728..3003
                     /locus_tag="IVK51_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP6430670.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IVK51_001062"
     CDS             3009..3776
                     /locus_tag="IVK51_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP6430671.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IVK51_001063"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IVK51_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP6430672.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IVK51_001064"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IVK51_001064"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP6430673.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IVK51_001065"
     CDS             7920..8222
                     /locus_tag="IVK51_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP6430674.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IVK51_001066"
     CDS             8225..9388
                     /locus_tag="IVK51_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP6430675.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IVK51_001067"
     CDS             9414..12842
                     /locus_tag="IVK51_001067"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP6430676.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IVK51_001068"
     CDS             12863..13987
                     /locus_tag="IVK51_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430677.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IVK51_001069"
     CDS             13977..14414
                     /locus_tag="IVK51_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6430678.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IVK51_001070"
     CDS             14398..14736
                     /locus_tag="IVK51_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP6430679.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IVK51_001071"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IVK51_001071"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP6430680.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IVK51_001072"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IVK51_001072"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP6430681.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IVK51_001073"
     CDS             15893..16936
                     /locus_tag="IVK51_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6430682.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IVK51_001074"
     CDS             17095..17823
                     /locus_tag="IVK51_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP6430683.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IVK51_001075"
     CDS             17939..18184
                     /locus_tag="IVK51_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6430684.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IVK51_001076"
     CDS             18181..18399
                     /locus_tag="IVK51_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP6430685.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IVK51_001077"
     CDS             18535..19074
                     /locus_tag="IVK51_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP6430686.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IVK51_001078"
     CDS             19067..21340
                     /locus_tag="IVK51_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP6430687.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IVK51_001079"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IVK51_001079"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP6430688.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IVK51_001080"
     CDS             complement(22513..22767)
                     /locus_tag="IVK51_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP6430689.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IVK51_001081"
     CDS             complement(23167..23436)
                     /locus_tag="IVK51_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430690.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IVK51_001082"
     CDS             complement(23906..24361)
                     /locus_tag="IVK51_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP6430691.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IVK51_001083"
     CDS             complement(24499..26427)
                     /locus_tag="IVK51_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP6430692.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IVK51_001084"
     CDS             complement(26624..27457)
                     /locus_tag="IVK51_001084"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP6430693.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IVK51_001085"
     CDS             complement(27649..28353)
                     /locus_tag="IVK51_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6430694.1"
                     /translation="MTETTLYIVRHGKPMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IVK51_001086"
     CDS             complement(28376..29002)
                     /locus_tag="IVK51_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6430695.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IVK51_001087"
     CDS             29328..31703
                     /locus_tag="IVK51_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP6430696.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IVK51_001088"
     CDS             complement(31727..32218)
                     /locus_tag="IVK51_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP6430697.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IVK51_001089"
     CDS             32405..33325
                     /locus_tag="IVK51_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6430698.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IVK51_001090"
     CDS             33479..33823
                     /locus_tag="IVK51_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP6430699.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IVK51_001091"
     CDS             complement(34013..34513)
                     /locus_tag="IVK51_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430700.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IVK51_001092"
     tRNA            35041..35124
                     /locus_tag="IVK51_001092"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IVK51_001093"
     CDS             complement(35448..>35882)
                     /locus_tag="IVK51_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6430701.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6261 c         7406 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag gcgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgttg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcggttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACCDF010000023        35688 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCDF010000023 DACCDF010000000
VERSION     DACCDF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.023"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(87..956)
                     /locus_tag="IVK51_001094"
     CDS             complement(87..956)
                     /locus_tag="IVK51_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP6430702.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(1415..3307)
                     /locus_tag="IVK51_001095"
     CDS             complement(1415..3307)
                     /locus_tag="IVK51_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430703.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(3304..5031)
                     /locus_tag="IVK51_001096"
     CDS             complement(3304..5031)
                     /locus_tag="IVK51_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430704.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(5047..5697)
                     /locus_tag="IVK51_001097"
     CDS             complement(5047..5697)
                     /locus_tag="IVK51_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6430705.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            5901..6197
                     /locus_tag="IVK51_001098"
                     /pseudo
     CDS             5901..6197
                     /locus_tag="IVK51_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            6369..6989
                     /locus_tag="IVK51_001099"
     CDS             6369..6989
                     /locus_tag="IVK51_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP6430706.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(7081..8247)
                     /locus_tag="IVK51_001100"
     CDS             complement(7081..8247)
                     /locus_tag="IVK51_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6430707.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(8259..8612)
                     /locus_tag="IVK51_001101"
     CDS             complement(8259..8612)
                     /locus_tag="IVK51_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6430708.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            8740..9762
                     /locus_tag="IVK51_001102"
     CDS             8740..9762
                     /locus_tag="IVK51_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP6430709.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(10201..10635)
                     /locus_tag="IVK51_001103"
     CDS             complement(10201..10635)
                     /locus_tag="IVK51_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430710.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(10745..12604)
                     /locus_tag="IVK51_001104"
     CDS             complement(10745..12604)
                     /locus_tag="IVK51_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6430711.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(12722..13882)
                     /locus_tag="IVK51_001105"
     CDS             complement(12722..13882)
                     /locus_tag="IVK51_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP6430712.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(14189..15613)
                     /gene="araA"
                     /locus_tag="IVK51_001106"
     CDS             complement(14189..15613)
                     /gene="araA"
                     /locus_tag="IVK51_001106"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP6430713.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(15629..16327)
                     /locus_tag="IVK51_001107"
     CDS             complement(15629..16327)
                     /locus_tag="IVK51_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6430714.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(16284..17945)
                     /locus_tag="IVK51_001108"
     CDS             complement(16284..17945)
                     /locus_tag="IVK51_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP6430715.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            18163..19563
                     /locus_tag="IVK51_001109"
     CDS             18163..19563
                     /locus_tag="IVK51_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP6430716.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(19815..20894)
                     /locus_tag="IVK51_001110"
     CDS             complement(19815..20894)
                     /locus_tag="IVK51_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6430717.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20980..21939)
                     /locus_tag="IVK51_001111"
     CDS             complement(20980..21939)
                     /locus_tag="IVK51_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP6430718.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21951..23474)
                     /locus_tag="IVK51_001112"
     CDS             complement(21951..23474)
                     /locus_tag="IVK51_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6430719.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(23484..24047)
                     /locus_tag="IVK51_001113"
     CDS             complement(23484..24047)
                     /locus_tag="IVK51_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP6430720.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(24053..24916)
                     /locus_tag="IVK51_001114"
     CDS             complement(24053..24916)
                     /locus_tag="IVK51_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6430721.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24930..25826)
                     /locus_tag="IVK51_001115"
     CDS             complement(24930..25826)
                     /locus_tag="IVK51_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430722.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(26001..27272)
                     /locus_tag="IVK51_001116"
     CDS             complement(26001..27272)
                     /locus_tag="IVK51_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430723.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(27262..28779)
                     /locus_tag="IVK51_001117"
     CDS             complement(27262..28779)
                     /locus_tag="IVK51_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP6430724.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28977..29867
                     /locus_tag="IVK51_001118"
     CDS             28977..29867
                     /locus_tag="IVK51_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP6430725.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29940..30764)
                     /gene="nadE"
                     /locus_tag="IVK51_001119"
     CDS             complement(29940..30764)
                     /gene="nadE"
                     /locus_tag="IVK51_001119"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP6430726.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(30776..32248)
                     /locus_tag="IVK51_001120"
     CDS             complement(30776..32248)
                     /locus_tag="IVK51_001120"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP6430727.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(32885..33247)
                     /locus_tag="IVK51_001121"
     CDS             complement(32885..33247)
                     /locus_tag="IVK51_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6430728.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            33470..34222
                     /locus_tag="IVK51_001122"
     CDS             33470..34222
                     /locus_tag="IVK51_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6430729.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(34362..35285)
                     /locus_tag="IVK51_001123"
     CDS             complement(34362..35285)
                     /locus_tag="IVK51_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6430730.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(35300..35572)
                     /locus_tag="IVK51_001124"
     CDS             complement(35300..35572)
                     /locus_tag="IVK51_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430731.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10519 a         7437 c         6123 g        11609 t
ORIGIN      
        1 attttctctt tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca
       61 tgcgacaatc gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt
      121 tcttttggct cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact
      181 gtgtgctttt tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct
      241 tccatgcttt cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta
      301 ggcaaatcaa ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc
      361 aatacatgga tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat
      421 tggcgttctt ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt
      481 gatagtttac tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct
      541 ggaatttctc tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct
      601 gggtctacga gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa
      661 ttcattttac ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat
      721 gaaaaacggt tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc
      781 agcagcaaca tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct
      841 cttttcaaat caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc
      901 acccgaatgg ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt
      961 cttgctcccc tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata
     1021 actttgattt tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt
     1081 aatgagtaaa aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa
     1141 gaacagccaa acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga
     1201 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta
     1261 ttagagatac aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg
     1321 aaggacagct gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca
     1381 cagcccctaa aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact
     1441 gactgttata aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg
     1501 tttctacgat cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca
     1561 agcggtgagc aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa
     1621 tcagtatttc tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg
     1681 gatttgctaa gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc
     1741 ttgcatcctc gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta
     1801 cgtgagcagc tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt
     1861 tgatattgtc atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact
     1921 gtgcgcgcaa ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac
     1981 ctccagaaac atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag
     2041 tcggacctac aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta
     2101 gcaagttatc tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat
     2161 ctttttcttc tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa
     2221 atacccgttc ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag
     2281 tgattggttg agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca
     2341 tgttcccatt tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt
     2401 taacgaaatt catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg
     2461 catacagatt ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg
     2521 ttttaacaat ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac
     2581 ttttttgctg atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca
     2641 agcttaaagg gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca
     2701 tcacgatgac cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca
     2761 gcgtgcttgc gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat
     2821 gtgtatcata ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt
     2881 cataaactgt tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag
     2941 cagaaatcaa atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta
     3001 tcggaaattt tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt
     3061 tgaatatttc tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg
     3121 ctgctattgc tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa
     3181 acaatcggcg taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc
     3241 tgccaggtcc accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt
     3301 tactcatgct aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt
     3361 tgttgctaat agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag
     3421 gatcatatct gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc
     3481 tttcatattc gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga
     3541 gaaggaatca tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag
     3601 acgctgtctt tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc
     3661 atcttccatc tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat
     3721 ttcatctgtt gcatccggct taccatactg catattgtct cggatcgttc cagagaacag
     3781 aactgctttt tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact
     3841 aatgtccgtt ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa
     3901 attgactaag gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga
     3961 cttggcttgg aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt
     4021 gacatgattg aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc
     4081 agggtcatgg ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg
     4141 tgcacgaggc accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat
     4201 cgcataagtc ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg
     4261 tccaccaaac caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat
     4321 catcaatgcg acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa
     4381 atcaaaacga ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt
     4441 caatccttct cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa
     4501 tggaacagca aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa
     4561 gaagattttt gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt
     4621 gattggtgca ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac
     4681 atcattggtc gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt
     4741 cagactctct gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc
     4801 acgagtagca aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat
     4861 catcacaaaa cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat
     4921 aatattggag gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac
     4981 tgcagctgca attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc
     5041 ttctttctat ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat
     5101 tgcttgctct actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt
     5161 tgtaaataca gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac
     5221 tttcagcttc gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt
     5281 atgtggatcg tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg
     5341 gtaatccatg ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc
     5401 ataaatcatt ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt
     5461 cattagttgt aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata
     5521 aagatcctct ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt
     5581 gacgatatta gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc
     5641 ctcgattagt cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg
     5701 gatcctcctt tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc
     5761 ctacctgttt tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa
     5821 gtgataagag gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata
     5881 ctaaacaaag gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc
     5941 ctctcgatgc tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc
     6001 acttaagcca ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag
     6061 atccaaagaa actttaatat attctaagct atacaggcag gcgatcagaa gcattagccg
     6121 atcaacttac tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc
     6181 agtcgagtgt tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt
     6241 gtcacagcct cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata
     6301 tgacttgata gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag
     6361 gtaagcatat ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc
     6421 gcacagttaa ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg
     6481 aagtgactgt tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat
     6541 caggttggga agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac
     6601 aaaaaattgc tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta
     6661 tcgcaaacgc cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg
     6721 tcaatgtagc tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag
     6781 gtaaaaaagc actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct
     6841 ctcaatatgt aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca
     6901 tcgaaggaat cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata
     6961 aagctgctgc tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag
     7021 aggaaaaaat cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg
     7081 ttattctttc tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca
     7141 ttcaaaaaca tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac
     7201 accgatgatt ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg
     7261 gattgctaac tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc
     7321 tactgcaaaa acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa
     7381 gatagccgga atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc
     7441 aataatcccc aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat
     7501 gattgccaca gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt
     7561 catttcactc agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa
     7621 ataaggtgct aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag
     7681 cagagccact aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact
     7741 cttagatata tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc
     7801 caactgttcg cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc
     7861 aatcgtatct acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt
     7921 agggatgatc actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat
     7981 gatactgtaa agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa
     8041 gtaatacaag attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat
     8101 tggtgcaaac aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac
     8161 aacaagtaag gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg
     8221 tctgttcttc ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt
     8281 cccaaaccaa tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct
     8341 tcttctggac aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg
     8401 gccatcggac cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct
     8461 gctttcaaag aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag
     8521 gcagctgttg ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct
     8581 gtcccaattt ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa
     8641 aacagtataa cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa
     8701 cgaaaactat ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta
     8761 ggaacctata cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa
     8821 gaagaattag ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa
     8881 agtaaagctg gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg
     8941 tacgataagg attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat
     9001 gtagcggttg atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta
     9061 aatgtctacc atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa
     9121 gaagcgacag gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact
     9181 cctgaccaac gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg
     9241 tccgaaaacg gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct
     9301 cgccatcttg tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc
     9361 acagtccgcg tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata
     9421 tcgaccttgc cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat
     9481 gatggcaagt tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt
     9541 tttgaagatg gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc
     9601 cgcgatttcg cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat
     9661 aatctgacat tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat
     9721 gtctatgtac cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa
     9781 atgaatcagc aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat
     9841 gggaaaatcg aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta
     9901 cagaactact ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc
     9961 tgaaaagaac atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa
    10021 aaaccgccga gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta
    10081 caaggttgtt gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat
    10141 aaaggtaata ttacatttat attagataac tctagttttt attgttatca aagatttctt
    10201 ttattttttc aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc
    10261 ttggtcttgt tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc
    10321 gatactttgg atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt
    10381 tttatgaatg cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc
    10441 ccgatatttt tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc
    10501 cagttcactt gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat
    10561 tccttctccg attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc
    10621 aatattcggt aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact
    10681 tgtttttatc ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc
    10741 attcttattc gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt
    10801 catattggat caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt
    10861 ctttatacca ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt
    10921 ctaatgataa aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat
    10981 tcgctgcaaa ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc
    11041 ctttactgtc gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt
    11101 ggatcgtatc tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc
    11161 tagaaagatt cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat
    11221 cttgcagata attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt
    11281 ctagattatc atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga
    11341 actgaggtgt tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag
    11401 aaatatatgg attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact
    11461 ggtcaaatcc taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat
    11521 ggatcgctgt cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat
    11581 ccatttttgg tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag
    11641 aaaaaccagt caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa
    11701 gcatattgcc actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag
    11761 atacttctac gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga
    11821 tgttcggcga ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt
    11881 cagtgatttc tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct
    11941 tcagattgta caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt
    12001 aaggtatcca tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga
    12061 aattactgat ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt
    12121 ggaatcgcct tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg
    12181 ttcctgcggc agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc
    12241 cgatttcagt gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt
    12301 tcatataatt tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag
    12361 ggacagatcc tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta
    12421 agaagaattt ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat
    12481 cattttgaca ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga
    12541 ttgctgctgt tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt
    12601 tcaaatgctt gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg
    12661 tacgaaattc gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc
    12721 tttatggcct ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta
    12781 gctgttgttt cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt
    12841 agacatgctc attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca
    12901 gcagagaaaa tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa
    12961 acggcgcttg gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga
    13021 acaaaagata atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct
    13081 gagtcgatcc ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt
    13141 ctggaaaatc agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat
    13201 atttatcaac aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt
    13261 ctatattcgc ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga
    13321 tgtaatccga ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg
    13381 catcgcggat agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt
    13441 ttgtgcctat cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca
    13501 cataagcact tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta
    13561 ccatacgact tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct
    13621 cttttactgc attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag
    13681 ctttttcaac gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg
    13741 gtgtaagcca ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat
    13801 catctattat tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat
    13861 caatcaaatg aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct
    13921 gcaattagac aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga
    13981 gtaggctgag tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag
    14041 agcaaaatat tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa
    14101 taagactaaa tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa
    14161 aactaagtgg ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta
    14221 attttgccca cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac
    14281 cgccacctgc ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata
    14341 acacacgtgc aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat
    14401 tgatcagcag tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg
    14461 ctttcccatc aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa
    14521 caacgattct tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca
    14581 agattgcttc ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt
    14641 tccctcgagc catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc
    14701 cagcaaaacc gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca
    14761 ttccatacag gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac
    14821 ggcgaatacc gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt
    14881 cataatcacc atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt
    14941 cttcgtctga tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag
    15001 tccaaccaaa ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca
    15061 tgttgtcacc aaaacgagcc acttttattc caaaactttc attataggct actgcaacat
    15121 ccatccaatc agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt
    15181 tattttgttt atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt
    15241 gatttaaatt catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg
    15301 tagctaaatg aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg
    15361 gtgagaaagt atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt
    15421 tcatgatttt tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag
    15481 gcaattggcc attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc
    15541 gcagtgcttc ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc
    15601 caatattcaa cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc
    15661 cgtgttttcg taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat
    15721 tgagctgtct ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta
    15781 ccgcactttg cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg
    15841 gaattgctaa gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt
    15901 catattcgta cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc
    15961 cataaaaagt atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc
    16021 agatcgtcgc ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat
    16081 ataaaacagc atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat
    16141 tgtccaagtc cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag
    16201 gtttgataac gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt
    16261 tgacgagtcc gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt
    16321 ccagcatttt ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg
    16381 atcccctcac ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt
    16441 gtgatggtga ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac
    16501 ttcccatgtc gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct
    16561 ccggctgtgt ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca
    16621 acttctggtg ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca
    16681 gatttcaata tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc
    16741 atgaaattcg ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca
    16801 tttactcctg taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc
    16861 gtatcgcctt ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc
    16921 atacccaaag aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag
    16981 cagttattcg tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt
    17041 tccggatgga cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca
    17101 gccgaaatgt tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca
    17161 tccccttctg gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga
    17221 taagcccctg attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa
    17281 atgtgatcca agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc
    17341 atttcttgat ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct
    17401 acgcctagta ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa
    17461 aaattaattt ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata
    17521 ctccatctct gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca
    17581 gtgattgtat ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct
    17641 agataaccgt gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca
    17701 tagatttcga atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata
    17761 tcttctaatg aataggtcca gataccattt tctagtttgt tttcccactc ataactacca
    17821 gcggcaatag gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac
    17881 tcaatcccta aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc
    17941 ttcataatga tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc
    18001 gcttacaatg attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata
    18061 caataaaaaa cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc
    18121 tgatcaaccg ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa
    18181 gtttcctcat cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat
    18241 attggtgtta tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa
    18301 gcttctttga taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct
    18361 ttatctggtc ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt
    18421 atttttatgg ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg
    18481 attgcagcaa ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct
    18541 tatatgtctg aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg
    18601 atgattgctt ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct
    18661 gaaacgatgg cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt
    18721 ttcggtgttt tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa
    18781 gaggcaaaaa gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa
    18841 caaatccagc tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt
    18901 ttagaaaaat atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt
    18961 caaggagcta atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat
    19021 gcagcgagcg atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct
    19081 ttgctctttt tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt
    19141 agcgtgatgg gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat
    19201 acaaattcgt tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca
    19261 tgggctccac tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga
    19321 gcatctggcc tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt
    19381 cctgtgatga ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc
    19441 tttttagggg ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag
    19501 gaaatcgaac aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct
    19561 taaagaggtt gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc
    19621 agtcacaagg aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg
    19681 accagttggt aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga
    19741 gcgatgacaa aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg
    19801 taaaaacccg tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt
    19861 tcatagagaa tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg
    19921 gcatcttgtc ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa
    19981 tggcttaaag aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca
    20041 ttttctgtca acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca
    20101 tctaaagctt gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt
    20161 tccgtggtga aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg
    20221 ctgataaaac ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac
    20281 agacgctgat gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca
    20341 tctaaacaaa tataagaggc atttaactct tcataagcag cattgatcat caccatcgga
    20401 atgccttgtt ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc
    20461 gtaggctcaa cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc
    20521 tccaattcgt gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat
    20581 tcattttcga ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg
    20641 acaccgatcg tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca
    20701 tatgtgcccg accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct
    20761 tgccgaatcg tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg
    20821 gttgtattta cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct
    20881 tgatattttg ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc
    20941 tgcaaggttt attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg
    21001 ttcaccaaat tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg
    21061 attaggattg tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa
    21121 aatatcaaag cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt
    21181 atttttttca gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa
    21241 aagattcttg tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact
    21301 accagaatac gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac
    21361 tgaaaaaccc cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt
    21421 taacgtccaa ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg
    21481 atcttgttgg gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa
    21541 actgaaagtt tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg
    21601 atctatatgc tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt
    21661 tttatgattg cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg
    21721 tgcccagatc aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc
    21781 agcatgttct aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg
    21841 aaccgatcct gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg
    21901 ttgccgaacg ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc
    21961 tttattctaa ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt
    22021 tctattgaaa aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg
    22081 tccaacagat cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca
    22141 tgttcaatcg ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga
    22201 ttgccgttat ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca
    22261 taaacagggc aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt
    22321 tgcatcacat aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa
    22381 ggtattgtgt ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa
    22441 atagccagcc ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg
    22501 atgggactac cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat
    22561 tcatcaaacg ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa
    22621 gtagcaacga tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct
    22681 gtatcatcac gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata
    22741 tagttatgca aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc
    22801 cattcagggt acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa
    22861 tcatccacta acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca
    22921 ctcttagccc ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg
    22981 ccaaaaggct ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga
    23041 aaattacaat attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc
    23101 gtgaatatcg gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa
    23161 gaatttggtt ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta
    23221 cctacagtat ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca
    23281 cctaattcta attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct
    23341 gtttcatggc ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat
    23401 gagccaaaca gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc
    23461 acttcttcgt ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca
    23521 agtttccata cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt
    23581 acaaatccta ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc
    23641 catgcacttt gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat
    23701 atccaggata aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt
    23761 acgattgtct gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga
    23821 ctaaggaaca agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata
    23881 tttcctgatc cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc
    23941 acgatagtaa taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca
    24001 gaaaccacaa tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt
    24061 tcattccaga catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta
    24121 gtaatggcag cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct
    24181 gccctgtaaa taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata
    24241 tcaatggata aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa
    24301 gagtaggttt gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat
    24361 caatgcgtgc tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga
    24421 agataccaaa tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc
    24481 caagcaaatt gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg
    24541 tcagcaaaac gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat
    24601 accccgcaag agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg
    24661 tatttttgat gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg
    24721 tatagctatc tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag
    24781 aagaaataat cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa
    24841 tcgttcgaat gattttttta ccactaactg cttttcgact attcacagta actgtaatcg
    24901 agctttctgg tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg
    24961 tacacattag tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct
    25021 gatgcatagc ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct
    25081 gtttctgtct taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc
    25141 atcgaaccaa tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc
    25201 gtaatgttaa aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa
    25261 gagggagaga ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc
    25321 caaacagtca tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg
    25381 atcggcaagc cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac
    25441 atagaaaaaa taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa
    25501 cgacaaaaac gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg
    25561 ataatttgtg ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact
    25621 gctcgttcat cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta
    25681 aagaaaatat tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta
    25741 aataataata aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga
    25801 ttccaatatt tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat
    25861 acaatcttgg tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa
    25921 taacacctat ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct
    25981 atcctgtata aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg
    26041 ttcatgagtg tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata
    26101 tcgttattga tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt
    26161 tgtttcgata attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa
    26221 tccaagtagg caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc
    26281 caatatttca aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg
    26341 gcatatccta cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag
    26401 gtagtcacat tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg
    26461 atttcaatgg ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat
    26521 tctggaccag tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc
    26581 tcaggagacg caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct
    26641 aacatccagt tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt
    26701 ttactactat cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt
    26761 tgttcagtat ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct
    26821 tgtacctgca tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt
    26881 tctttaaaat cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc
    26941 gctccgtttt ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct
    27001 tttttagaag aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc
    27061 atttcaattt tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat
    27121 atttcaccat cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa
    27181 tcagattctg ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac
    27241 aaagagccaa ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg
    27301 acatctgacc acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc
    27361 ttttacattt gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt
    27421 tgtatcaaat ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa
    27481 gtgaacagtt agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt
    27541 gtcatttatt acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata
    27601 gtttgctgaa cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat
    27661 aaatggataa tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc
    27721 aataacgttt accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa
    27781 agtgattaat agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa
    27841 aggaggagca atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat
    27901 attccattca tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt
    27961 atcgcacatc gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata
    28021 atgagaaaga tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg
    28081 atcgtacgct tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt
    28141 cgaacttccg cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt
    28201 ttcctctgct aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc
    28261 catttcattc cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg
    28321 ccttaaatca ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc
    28381 tgcatctact ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg
    28441 acaccagtta taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa
    28501 atccaacctt ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga
    28561 cacaaaattt ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat
    28621 gacgtcttgg cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc
    28681 atatactgct cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc
    28741 tacacgcttg acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat
    28801 acatgaataa tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat
    28861 tttttatcga ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata
    28921 acttttatta acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc
    28981 aactacaaat aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga
    29041 tcaagatcat ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac
    29101 ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca
    29161 tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata
    29221 ttagttttcc agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg
    29281 gtcactatac aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta
    29341 tcgggaaagt ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc
    29401 tctggtttac cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata
    29461 cattggagat gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca
    29521 attggccttc agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc
    29581 ctggagattt tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta
    29641 atcaatacgg cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag
    29701 aacccttgtc aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc
    29761 gaatcgaagt gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat
    29821 tcggtaatta cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc
    29881 tattttataa aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt
    29941 tatttccaga aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc
    30001 caccatgctt cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca
    30061 tcaatttcat tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct
    30121 tcaagatctg cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc
    30181 aattgtttcc cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca
    30241 ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct
    30301 cctgctcttt ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt
    30361 cctttgttaa aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca
    30421 acagctggtt tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct
    30481 tttgcatctt cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag
    30541 ttttcatctt ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct
    30601 aaagtagaat cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt
    30661 ttcaaatatg ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt
    30721 ggtttgacac ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg
    30781 cttctgtatg aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt
    30841 tccagcattc agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact
    30901 cttcccatag tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta
    30961 attcgtaaac tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt
    31021 ctcgaacagt tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt
    31081 ctcgagggtc ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc
    31141 gagtgatgcg ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata
    31201 acttgatcgt atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg
    31261 caccaagggc tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa
    31321 ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat
    31381 agatttttgc ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat
    31441 aggccatatc tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt
    31501 tcgccacgcg aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga
    31561 acacgcagtc tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag
    31621 attggactaa cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac
    31681 gtacattgct tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg
    31741 catccagttc ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag
    31801 atttgatacg agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg
    31861 ctgtttctac taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg
    31921 caaaaactag ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta
    31981 aatactccaa aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat
    32041 cattgaacgt cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt
    32101 atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc
    32161 gacctaattc ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca
    32221 aactgtcgtc tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt
    32281 cctacccatt ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa
    32341 gcacatgttt tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt
    32401 cttattggta tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca
    32461 cgaagaaatt agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac
    32521 aatcgtagcc gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata
    32581 atcaatggca aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca
    32641 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
    32701 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
    32761 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
    32821 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
    32881 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
    32941 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
    33001 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
    33061 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
    33121 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
    33181 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
    33241 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
    33301 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
    33361 cttttatcac ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac
    33421 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
    33481 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
    33541 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
    33601 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
    33661 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
    33721 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
    33781 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
    33841 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
    33901 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
    33961 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
    34021 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag
    34081 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
    34141 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
    34201 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
    34261 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
    34321 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
    34381 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
    34441 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
    34501 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
    34561 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
    34621 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
    34681 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
    34741 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
    34801 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
    34861 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
    34921 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
    34981 tctttctgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
    35041 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
    35101 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
    35161 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
    35221 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
    35281 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
    35341 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
    35401 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
    35461 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
    35521 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
    35581 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
    35641 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttat
//
LOCUS       DACCDF010000024        35657 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCDF010000024 DACCDF010000000
VERSION     DACCDF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.024"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..2198)
                     /locus_tag="IVK51_001125"
     CDS             complement(<1..2198)
                     /locus_tag="IVK51_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430732.1"
                     /translation="MKIIKKKIANRKTNWLPVIFLVLIAVILFTSNIAEGSIFNWFDD
                     TESQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESTGLNKVYQAVLSTIVVGLMILSLVIIGYKMITGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDTTTITNSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVEGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLGTGSGSSVNPFIKAVA
                     YVCAVIVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDMANDTKNKFKEGLDDGTVYAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNINPASAIQQKVKQDIEERKNAMHGQAKS
                     AEVLINQKRQEAKYTPEAMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKSVKENLQNSTSSQDRRSVDVRENLQNTTSSQNGNRTVYVRENVQSSGT"
     gene            complement(2185..2403)
                     /locus_tag="IVK51_001126"
     CDS             complement(2185..2403)
                     /locus_tag="IVK51_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430733.1"
                     /translation="MIENHLVDKIHCLDQNADTLELTESICSNTIVLDLKAVNDDYVT
                     HIIIDRKIAQKLSESLRKWAEGGNNENN"
     gene            complement(2405..3448)
                     /locus_tag="IVK51_001127"
     CDS             complement(2405..3448)
                     /locus_tag="IVK51_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6430734.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKNKMQMFPNFYYLLKPVLCKDDVYKINKITHQENSQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLFESLS
                     TKNNKLDEELIETFIEENLLKQMWGESIVNCLKLASNSDYRQFDNWYKKFNYAIKSAE
                     KEQKVQLKIIYEICNNSYFVDHVREQLAMTLRDLIRRAKTDHRIKQKNNYIFTSLKNK
                     ALELIKLQKKEGI"
     gene            complement(3603..3929)
                     /locus_tag="IVK51_001128"
     CDS             complement(3603..3929)
                     /locus_tag="IVK51_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430735.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3937..4140)
                     /locus_tag="IVK51_001129"
     CDS             complement(3937..4140)
                     /locus_tag="IVK51_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430736.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKSK"
     gene            4215..4865
                     /locus_tag="IVK51_001130"
     CDS             4215..4865
                     /locus_tag="IVK51_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430737.1"
                     /translation="MSIKTLLNLWFLCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4920..6086
                     /locus_tag="IVK51_001131"
     CDS             4920..6086
                     /locus_tag="IVK51_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6430738.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IVK51_001132"
     CDS             complement(6175..6567)
                     /gene="rpsI"
                     /locus_tag="IVK51_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP6430739.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IVK51_001133"
     CDS             complement(6583..7026)
                     /gene="rplM"
                     /locus_tag="IVK51_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP6430740.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(7545..8012)
                     /locus_tag="IVK51_001134"
     CDS             complement(7545..8012)
                     /locus_tag="IVK51_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP6430741.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(8132..9670)
                     /locus_tag="IVK51_001135"
     CDS             complement(8132..9670)
                     /locus_tag="IVK51_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP6430742.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            9750..10436
                     /locus_tag="IVK51_001136"
     CDS             9750..10436
                     /locus_tag="IVK51_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP6430743.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IVK51_001137"
     CDS             complement(10637..11164)
                     /gene="msrA"
                     /locus_tag="IVK51_001137"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6430744.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IVK51_001138"
     CDS             complement(11248..11925)
                     /gene="pgmB"
                     /locus_tag="IVK51_001138"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP6430745.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(11922..13052)
                     /locus_tag="IVK51_001139"
     CDS             complement(11922..13052)
                     /locus_tag="IVK51_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6430746.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(13052..15235)
                     /locus_tag="IVK51_001140"
     CDS             complement(13052..15235)
                     /locus_tag="IVK51_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP6430747.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(15232..16257)
                     /locus_tag="IVK51_001141"
     CDS             complement(15232..16257)
                     /locus_tag="IVK51_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6430748.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(16302..17129)
                     /locus_tag="IVK51_001142"
     CDS             complement(16302..17129)
                     /locus_tag="IVK51_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6430749.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(17126..18193)
                     /locus_tag="IVK51_001143"
     CDS             complement(17126..18193)
                     /locus_tag="IVK51_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP6430750.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(18219..19055)
                     /locus_tag="IVK51_001144"
     CDS             complement(18219..19055)
                     /locus_tag="IVK51_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6430751.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(19052..19921)
                     /locus_tag="IVK51_001145"
     CDS             complement(19052..19921)
                     /locus_tag="IVK51_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430752.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(19991..21274)
                     /locus_tag="IVK51_001146"
     CDS             complement(19991..21274)
                     /locus_tag="IVK51_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6430753.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(21288..22973)
                     /locus_tag="IVK51_001147"
     CDS             complement(21288..22973)
                     /locus_tag="IVK51_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP6430754.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            23248..24273
                     /locus_tag="IVK51_001148"
     CDS             23248..24273
                     /locus_tag="IVK51_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6430755.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IVK51_001149"
     CDS             complement(24868..28521)
                     /gene="rpoC"
                     /locus_tag="IVK51_001149"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP6430756.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKSVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IVK51_001150"
     CDS             complement(28600..32217)
                     /gene="rpoB"
                     /locus_tag="IVK51_001150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP6430757.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            32945..33913
                     /locus_tag="IVK51_001151"
     CDS             32945..33913
                     /locus_tag="IVK51_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6430758.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            33931..34836
                     /locus_tag="IVK51_001152"
     CDS             33931..34836
                     /locus_tag="IVK51_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6430759.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            34833..35639
                     /locus_tag="IVK51_001153"
     CDS             34833..35639
                     /locus_tag="IVK51_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6430760.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
BASE COUNT        10106 a         7531 c         6435 g        11585 t
ORIGIN      
        1 cttgtcccac tactttgtac attttctcgt acatatactg ttcgatttcc attttgacta
       61 cttgtagtat tttgtaagtt ttctcgaaca tctactgagc gcctatcttg actacttgta
      121 gaattttgta gattttcttt gactgatttt tctaatccgg tatttgaatt tttagcttct
      181 tggattcttt ctttagtcaa cgcttctttt tcgtccatat tagcgcctgt acgcccttct
      241 acacgtttcc ttaacatttc ctcacgattc attgcttcag gcgtgtattt tgcttcctgt
      301 cgcttctgat taattaatac ttcagcagac ttagcttgac cgtgcatagc atttttacgt
      361 tcttcaatat cttgtttaac tttttgctga attgcacttg caggattaat atttgattca
      421 gcattatttt tcattgcttc acgcatacgt tcagaactag acataattct atcagcgttt
      481 tcaactttag cgccatcttt tctatccgaa agacgtgttg gcatagaatc agcatatttt
      541 ccagtttcat cttctttgtt tttagctact ataggtttgc tatttttatt aatagcgtac
      601 acggtaccat catctaaacc ttctttgaat ttattcttgg tatcattagc catatcacgc
      661 atactcttaa atggttttgt tgctctttca ctggccattg tagctccatc cgaagctaag
      721 cctgataatc ctcgttcacg agcataccca agtgttttcc ccgttttatt agctttttga
      781 gaaagtgatt gaccaaagtt gcgttcaggt tttcgttctt ccccttccgc ttgatcgttt
      841 ccaccactat ttttcccacc tttagatcgg ttcataccac ctgttccttt acgaaacagc
      901 atagctccca tgccaaatgc tgaagcaagc tggccatagc cttcttttaa accaatatcc
      961 acgccaaagt aacgcatgac tgttccacta ccctttatca gcacaataac agcgcagacg
     1021 tacgctactg cttttataaa aggatttaca gaagaaccgc tacctgttcc gagataatta
     1081 acaaacattg caaacataga caatccgaac ccttgaaatc cgactgtcaa ataacactgt
     1141 aaaatatctg acaaaacttg ctttgatcgt tggcctgttt ccaagtcagt agcaaatact
     1201 aaaactccaa gtacgcgttt aaacgctagc tcgagtattg ctgtaattat aatgaaagca
     1261 ctaaaaacat acgctaccgc taaagcaatc aatccaaaag aaatccccca tagatttgct
     1321 tgatagcgat aatatcctga tttaatgccc tcaacaaagg tcgacagaaa attatccgaa
     1381 aatttggtag caacaaattc attatcagaa ttttcaacta attcataacg cagattatca
     1441 gctattgggt tctctttttc cattttgtcg agtacattat ctgtcaatac ttgcgtaaga
     1501 tccgttttcc aaaaattttc ttttgttaga ttgttccggt cattcgtttt atcgatcatg
     1561 gaatattgat ctgttttatt gatataagct aaatcagtaa ccccttgttc aatcagcgac
     1621 cacgccacgc cttcgtccga atttgtaatc gtagtcgtat cattataaaa tgttttagaa
     1681 aactctattc cgctgtgaat caacgttggc attaaaagaa tcaatgaaat tgacattaca
     1741 acattcattc caacagattt tagatcaacc gttcccttac ctgtaatcat tttataacca
     1801 ataatcacta gtgaaagtat cattaagcca acaacaatgg tacttaaaac agcttgatag
     1861 actttgttca atccggtact ttctacaaag ctaaacaaat ctaaaacttc gggaatcata
     1921 ttttgaatgc catcgacaac agcgaacatt gattttataa cagaccagcc tatccacata
     1981 ccgaactggc tgtaaaaatt tccgttgtat tttttcaaat aattctggta aagcggatta
     2041 gttaaaaact cagtttgact ctcagtatca tcaaaccaat tgaaaatact gccttctgca
     2101 atgttactcg taaaaagaat cacagctatt agaacaagaa aaataaccgg taaccaattt
     2161 gtttttctat tagcaatctt ttttttaatt attttcattg ttacctccct cagcccattt
     2221 tcttaggctc tcagagagtt tctgagctat ttttctgtca attatgatat gagttacgta
     2281 atcatcatta acagctttta aatcgagaac aatagtattt gagcagatac tctcggttaa
     2341 ctcaagagta tctgcgtttt gatcaagaca gtgaatctta tctactagat gattttcaat
     2401 cattttaaat tccctctttt ttttgtagct tgattagttc taacgctttg ttttttagag
     2461 aagtaaagat ataattattt ttttgtttga tacgatgatc tgtttttgct cttcgtatca
     2521 agtcacgtaa agtcattgct agctgttctc taacatgatc tacaaaataa ctattattac
     2581 aaatttcata gataattttc agttgaactt tttgttcttt ttcagcagat ttaattgcat
     2641 aattgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta gccagtttta
     2701 agcaattcac aatactttct ccccacattt gtttaagaag attttcttca ataaatgttt
     2761 cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaaa agatcatcat
     2821 tatgtgattc tttaatatct ttactatctt tataagactt atataattct agttcatttt
     2881 ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg catagttcaa
     2941 attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga actatcaagt
     3001 ttttttgact attttcttga tgggttatct tattaatttt ataaacatca tctttgcaca
     3061 gtacaggctt taacaaataa taaaaatttg ggaacatttg cattttattt ttattgaagc
     3121 cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct gataagattg
     3181 tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc aaaaaaattt
     3241 tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct tttagtaaca
     3301 tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg tttaaaaaaa
     3361 taggatattg atagtataat tctgcatata tatcagaatt agaatagcct tgcttttcaa
     3421 ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt atttgctata
     3481 atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg atctgcaaac
     3541 tgacgggaac actcttttta attttttcta cttcatcatt attcaaaaaa acacctcgtt
     3601 ttttaaattt gatgactcaa tatccatttt ttgcattctt cttttgaaaa caactccatt
     3661 ttttctaata aaataactgg caatcccatt cttttaaatt tcaaaaaagt tgtatatgat
     3721 acattcaaat atttagccat ctcttttcct tttagaaatg gtctttctaa cccggtatca
     3781 attcttgctt gatcaatagc ctcttttgtc atttcaaaaa cataattttt caattgattc
     3841 gtttgttgtt tatctaacat tactgtaatt cctgcatcca ttatttttct cctctcattt
     3901 tctatttctt caatcagcca actcgccaag ttgtatttat ttagatttca aatcattaag
     3961 taaccaattt accaactttt gataagtaga tgctctaata ctttctttgc tcccattttc
     4021 gattttgaca tatgttttcc gatttatacc tatcaatttt gctgcatcgt ttttagtaag
     4081 tataagatcg gctctatgtc ttcttaagct tctaatgaat tgatcattaa gagttatcat
     4141 tcagcgcctc cttttaaatt attcactttg tggtttttat cttaataagc acattttaaa
     4201 ccacgaaacg agtaatgtca attaaaacat tactcaattt gtggttttta tgctataata
     4261 gtttggaatt tactataggg ggtgttactt tgaatagaat aaaattttat attgattcca
     4321 gtaaaaagac cattaaacaa attagtacag aaactggtat taactataca actcttacga
     4381 actattaccg tggtgagcga aatcctagaa atactgaaac atggaatatc cttgctgatt
     4441 atttccatgt tcctgtaagt tatttgatgg gattagacga aacaaattcc atagaacaac
     4501 ctaatcttga tagaataaaa actgctcgtt tagaaaaaaa ggacaaaata gtcaagaata
     4561 ttgccagtct tgactcagat tattttactg aattatctaa tctatacttt tccatgactt
     4621 ctttacttga agtcaaactt caagaagatg actccgactt tcttttttta aaaagtattc
     4681 tagaggactc ctttgaatac acgattctat ttgccaaaca aaagaagttt attaataaag
     4741 ctttaaactt aaatatcgat gaaagaaaag agttagaatc tgagttattt ggcaacttaa
     4801 cccaagtaca ggttaacaat ctaaaaagag agataacagc tcgattaata tcaaaacttc
     4861 aataaaacta attattcaat cagccaactc gccaagttat cagattggag agattagtta
     4921 tgtcaataca aaagtacaca acaaaaagcg gtgcacgata tagtgtccgc ttgtatgttc
     4981 gtaaagatga aaatggaaag caagaatact atgtaaaaaa agggtttaaa actgaaaaag
     5041 aagccaagtt acacgaagca agaaaaaagg tcgagattga agataccggc tatcataagc
     5101 cttctaatga cttgtttata acagtgtatg aagaatggtt gccttcttat aggaatcgtg
     5161 tacaagaaac gacatatcaa cgtacaaaag accttttccg cctgcatata ctaccaaaat
     5221 tcgggaataa gtccatttct aagattactc ctgttcactg tcagaatgca gtcaatgaat
     5281 gggctttaaa attcaagaat atcaaacagc ttaagtctta cagctctcaa atatttgagt
     5341 atgccatttt tgcagagcta ttacaccgaa atcctatggc aaatacaaat ctaccaaagc
     5401 gggaaaagaa agaaaatgaa aattactttt cattagaaga actaaaagct ttttgggata
     5461 ttctcaataa agaagagcct ttaaaacata ttttaatctt ccagcttttg attactactg
     5521 gtatacgtaa aggagagcta gctgctcttc attggtctga catttatttt gaagaacagc
     5581 taatgtatgt tagaaagagc tatgcaacta ttcgaaatga agagaagaat gcaagaagaa
     5641 aaactgtcag gattcaaaaa aatacaaaaa atatatcttc cgaacgaata ttaccaattg
     5701 atgcacaaac tattgaaatg ttaaaaatct ggaaaaaaga acaagctcga gagcttttgc
     5761 agtttggcat aaatacaaat acaaaaaatc aacttatctt tacctatgta aatgcagacg
     5821 gtgacattaa tcaacctttg catgcggact attcaaataa tatattgaaa cgtttagaga
     5881 agaaatataa atttaagcac gttactattc atggattccg gcacactcac gctactcttc
     5941 tactagaagg tggagcaagt atcaaagaaa cacaagatcg attgggtcat aaaaatgctg
     6001 aaactaccct taacacttat tctcatgtta ctgaaaaagc tcaacgaaat gcgttagaaa
     6061 aattcattgc ttacactggt ttataatttt gtcattacct ttctcattac cagaacgaaa
     6121 aaaagacacc ttctcgcaat gagaaagtgt ctgaaatcgt tggtataaca acgattaacg
     6181 ttttgaaaac tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     6241 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     6301 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     6361 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     6421 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     6481 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     6541 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     6601 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     6661 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     6721 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     6781 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     6841 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     6901 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     6961 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     7021 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     7081 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     7141 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     7201 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     7261 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     7321 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     7381 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     7441 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     7501 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     7561 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     7621 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     7681 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     7741 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     7801 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     7861 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     7921 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     7981 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     8041 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     8101 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     8161 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     8221 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     8281 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     8341 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     8401 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     8461 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     8521 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     8581 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     8641 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     8701 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     8761 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     8821 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     8881 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     8941 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     9001 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     9061 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     9121 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     9181 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     9241 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     9301 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     9361 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     9421 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     9481 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     9541 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     9601 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     9661 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     9721 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     9781 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     9841 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     9901 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     9961 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
    10021 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
    10081 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
    10141 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
    10201 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
    10261 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
    10321 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
    10381 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
    10441 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
    10501 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
    10561 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
    10621 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
    10681 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
    10741 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
    10801 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
    10861 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
    10921 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
    10981 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
    11041 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
    11101 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
    11161 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
    11221 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
    11281 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
    11341 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
    11401 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
    11461 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
    11521 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
    11581 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
    11641 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
    11701 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
    11761 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
    11821 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
    11881 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
    11941 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
    12001 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
    12061 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
    12121 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
    12181 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
    12241 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
    12301 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
    12361 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
    12421 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
    12481 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
    12541 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
    12601 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
    12661 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
    12721 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
    12781 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
    12841 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
    12901 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
    12961 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
    13021 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
    13081 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
    13141 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
    13201 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
    13261 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
    13321 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
    13381 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
    13441 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
    13501 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
    13561 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
    13621 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
    13681 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
    13741 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
    13801 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
    13861 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
    13921 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
    13981 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
    14041 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
    14101 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
    14161 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
    14221 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
    14281 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
    14341 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
    14401 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
    14461 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
    14521 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
    14581 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
    14641 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
    14701 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
    14761 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
    14821 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
    14881 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
    14941 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    15001 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    15061 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    15121 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    15181 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    15241 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    15301 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    15361 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    15421 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    15481 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    15541 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    15601 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    15661 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    15721 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    15781 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    15841 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    15901 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    15961 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    16021 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    16081 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    16141 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    16201 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    16261 tttctccctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    16321 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    16381 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    16441 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    16501 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    16561 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    16621 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    16681 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    16741 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    16801 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    16861 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    16921 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    16981 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    17041 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    17101 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    17161 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    17221 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    17281 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    17341 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    17401 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatccctc
    17461 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    17521 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    17581 aatcggcacc gtatttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    17641 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    17701 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    17761 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    17821 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    17881 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    17941 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    18001 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    18061 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    18121 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    18181 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    18241 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    18301 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    18361 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    18421 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    18481 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    18541 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    18601 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    18661 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    18721 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    18781 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    18841 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    18901 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    18961 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    19021 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    19081 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    19141 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    19201 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    19261 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    19321 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    19381 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    19441 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    19501 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    19561 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    19621 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    19681 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    19741 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    19801 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    19861 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    19921 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    19981 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    20041 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    20101 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    20161 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    20221 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    20281 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    20341 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    20401 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    20461 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    20521 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    20581 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    20641 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    20701 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    20761 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    20821 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    20881 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    20941 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    21001 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    21061 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    21121 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    21181 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    21241 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    21301 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    21361 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    21421 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    21481 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    21541 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    21601 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    21661 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    21721 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    21781 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    21841 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    21901 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    21961 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    22021 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    22081 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    22141 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    22201 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    22261 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    22321 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    22381 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    22441 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    22501 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    22561 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    22621 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    22681 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    22741 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    22801 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    22861 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    22921 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    22981 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    23041 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    23101 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    23161 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    23221 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    23281 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    23341 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    23401 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    23461 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    23521 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    23581 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    23641 atggatgaaa cacttctttt tgaattggat caatacaata ttcctattgt ggtaatcgga
    23701 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    23761 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    23821 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    23881 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    23941 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    24001 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    24061 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    24121 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    24181 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    24241 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    24301 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    24361 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    24421 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    24481 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    24541 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    24601 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    24661 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    24721 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    24781 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    24841 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    24901 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    24961 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    25021 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    25081 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    25141 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    25201 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    25261 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    25321 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    25381 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    25441 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    25501 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    25561 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    25621 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    25681 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    25741 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    25801 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    25861 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    25921 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    25981 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    26041 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    26101 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    26161 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    26221 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    26281 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    26341 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    26401 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    26461 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    26521 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    26581 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    26641 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    26701 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    26761 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    26821 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    26881 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    26941 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    27001 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    27061 tgatttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    27121 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    27181 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    27241 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    27301 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    27361 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    27421 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    27481 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    27541 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    27601 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    27661 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    27721 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    27781 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    27841 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    27901 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    27961 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    28021 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    28081 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    28141 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    28201 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    28261 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    28321 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    28381 gaacaaccct tcgcgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    28441 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    28501 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    28561 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    28621 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    28681 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    28741 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    28801 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    28861 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    28921 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    28981 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    29041 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    29101 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    29161 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    29221 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    29281 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    29341 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    29401 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    29461 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    29521 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    29581 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    29641 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    29701 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    29761 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    29821 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    29881 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    29941 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    30001 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    30061 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    30121 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    30181 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    30241 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    30301 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    30361 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    30421 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    30481 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    30541 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    30601 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    30661 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    30721 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    30781 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    30841 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    30901 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    30961 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    31021 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    31081 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    31141 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    31201 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    31261 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    31321 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    31381 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    31441 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    31501 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    31561 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    31621 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    31681 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    31741 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    31801 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    31861 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    31921 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    31981 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    32041 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    32101 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    32161 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    32221 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    32281 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    32341 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    32401 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    32461 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    32521 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    32581 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    32641 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    32701 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    32761 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    32821 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    32881 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    32941 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    33001 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    33061 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    33121 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    33181 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    33241 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    33301 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    33361 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    33421 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    33481 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    33541 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    33601 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    33661 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    33721 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    33781 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    33841 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    33901 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    33961 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    34021 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    34081 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    34141 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    34201 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    34261 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    34321 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    34381 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    34441 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    34501 tctaatggat gcattgctct ttctggctcc ttgcttgcgc aaaacaacgg ctttgctgat
    34561 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    34621 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    34681 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    34741 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    34801 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    34861 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    34921 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    34981 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    35041 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    35101 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    35161 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    35221 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    35281 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    35341 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    35401 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    35461 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    35521 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    35581 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    35641 cagaaagagg atgggac
//
LOCUS       DACCDF010000025        35595 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCDF010000025 DACCDF010000000
VERSION     DACCDF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.025"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            2..400
                     /locus_tag="IVK51_001154"
     CDS             2..400
                     /locus_tag="IVK51_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP6430761.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IVK51_001155"
     CDS             384..1079
                     /locus_tag="IVK51_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP6430762.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1663..2550)
                     /locus_tag="IVK51_001156"
     CDS             complement(1663..2550)
                     /locus_tag="IVK51_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP6430763.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2735..3574
                     /locus_tag="IVK51_001157"
     CDS             2735..3574
                     /locus_tag="IVK51_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6430764.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK51_001158"
     CDS             3770..4744
                     /gene="sgrA"
                     /locus_tag="IVK51_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP6430765.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5042..5398
                     /locus_tag="IVK51_001159"
     CDS             5042..5398
                     /locus_tag="IVK51_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430766.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5401..6558
                     /locus_tag="IVK51_001160"
     CDS             5401..6558
                     /locus_tag="IVK51_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430767.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6584..7024)
                     /locus_tag="IVK51_001161"
     CDS             complement(6584..7024)
                     /locus_tag="IVK51_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6430768.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7184..7690
                     /locus_tag="IVK51_001162"
     CDS             7184..7690
                     /locus_tag="IVK51_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6430769.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7858..9771
                     /locus_tag="IVK51_001163"
     CDS             7858..9771
                     /locus_tag="IVK51_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6430770.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9776..10777
                     /locus_tag="IVK51_001164"
     CDS             9776..10777
                     /locus_tag="IVK51_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6430771.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10979..11185)
                     /locus_tag="IVK51_001165"
     CDS             complement(10979..11185)
                     /locus_tag="IVK51_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP6430772.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11831..12679)
                     /locus_tag="IVK51_001166"
     CDS             complement(11831..12679)
                     /locus_tag="IVK51_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6430773.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12843..13745
                     /gene="gnd"
                     /locus_tag="IVK51_001167"
     CDS             12843..13745
                     /gene="gnd"
                     /locus_tag="IVK51_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP6430774.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13747..14748
                     /locus_tag="IVK51_001168"
     CDS             13747..14748
                     /locus_tag="IVK51_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6430775.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14745..15029
                     /locus_tag="IVK51_001169"
     CDS             14745..15029
                     /locus_tag="IVK51_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6430776.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15062..15859
                     /locus_tag="IVK51_001170"
     CDS             15062..15859
                     /locus_tag="IVK51_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6430777.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15873..16706
                     /locus_tag="IVK51_001171"
     CDS             15873..16706
                     /locus_tag="IVK51_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6430778.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16724..17581
                     /locus_tag="IVK51_001172"
     CDS             16724..17581
                     /locus_tag="IVK51_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6430779.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17603..18112
                     /locus_tag="IVK51_001173"
     CDS             17603..18112
                     /locus_tag="IVK51_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6430780.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18149..18550
                     /locus_tag="IVK51_001174"
     CDS             18149..18550
                     /locus_tag="IVK51_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430781.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18662..19015
                     /locus_tag="IVK51_001175"
     CDS             18662..19015
                     /locus_tag="IVK51_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430782.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19049..19492
                     /locus_tag="IVK51_001176"
     CDS             19049..19492
                     /locus_tag="IVK51_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6430783.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK51_001177"
     CDS             19509..20594
                     /gene="uxuA"
                     /locus_tag="IVK51_001177"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP6430784.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20587..21300
                     /locus_tag="IVK51_001178"
     CDS             20587..21300
                     /locus_tag="IVK51_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6430785.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            21629..22930
                     /locus_tag="IVK51_001179"
     CDS             21629..22930
                     /locus_tag="IVK51_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6430786.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            23553..23753
                     /locus_tag="IVK51_001180"
     CDS             23553..23753
                     /locus_tag="IVK51_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6430787.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            24321..24884
                     /locus_tag="IVK51_001181"
     CDS             24321..24884
                     /locus_tag="IVK51_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430788.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(24945..25289)
                     /locus_tag="IVK51_001182"
     CDS             complement(24945..25289)
                     /locus_tag="IVK51_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430789.1"
                     /translation="MISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFHYSPYRLF
                     QYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHDEVYLAEC
                     LANLEQKGIYLT"
     gene            complement(25386..25631)
                     /locus_tag="IVK51_001183"
     CDS             complement(25386..25631)
                     /locus_tag="IVK51_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430790.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(25701..25955)
                     /locus_tag="IVK51_001184"
     CDS             complement(25701..25955)
                     /locus_tag="IVK51_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430791.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(26119..26442)
                     /locus_tag="IVK51_001185"
     CDS             complement(26119..26442)
                     /locus_tag="IVK51_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430792.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            26629..26702
                     /locus_tag="IVK51_001186"
     tRNA            26629..26702
                     /locus_tag="IVK51_001186"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26663..26665,aa:Ile,seq:cat)
     gene            complement(27087..27584)
                     /locus_tag="IVK51_001187"
     CDS             complement(27087..27584)
                     /locus_tag="IVK51_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6430793.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(27624..28433)
                     /locus_tag="IVK51_001188"
     CDS             complement(27624..28433)
                     /locus_tag="IVK51_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6430794.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIDMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            28767..29624
                     /locus_tag="IVK51_001189"
     CDS             28767..29624
                     /locus_tag="IVK51_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6430795.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            29617..30519
                     /locus_tag="IVK51_001190"
     CDS             29617..30519
                     /locus_tag="IVK51_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6430796.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            30552..30902
                     /locus_tag="IVK51_001191"
     CDS             30552..30902
                     /locus_tag="IVK51_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6430797.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            30936..31874
                     /locus_tag="IVK51_001192"
     CDS             30936..31874
                     /locus_tag="IVK51_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6430798.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            31952..32845
                     /locus_tag="IVK51_001193"
     CDS             31952..32845
                     /locus_tag="IVK51_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6430799.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            32883..33446
                     /gene="amaP"
                     /locus_tag="IVK51_001194"
     CDS             32883..33446
                     /gene="amaP"
                     /locus_tag="IVK51_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP6430800.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            33460..33648
                     /locus_tag="IVK51_001195"
     CDS             33460..33648
                     /locus_tag="IVK51_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP6430801.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            33661..34563
                     /locus_tag="IVK51_001196"
     CDS             33661..34563
                     /locus_tag="IVK51_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6430802.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            34595..34837
                     /locus_tag="IVK51_001197"
     CDS             34595..34837
                     /locus_tag="IVK51_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6430803.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(35093..35464)
                     /locus_tag="IVK51_001198"
     CDS             complement(35093..35464)
                     /locus_tag="IVK51_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430804.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT        12348 a         5718 c         7129 g        10400 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattactt atttctcatt taaacgatgt
     1201 attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt gaataaattt
     1261 cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct agtataacag
     1321 aataagagga acaagtccag aagggatatt tttagtcttt tttaaaataa cttttcagtc
     1381 gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa atagggaagc
     1441 atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa acttttttaa
     1501 attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat aaaaaaaggc
     1561 cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt tccaaaaatc
     1621 acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga atatattttt
     1681 tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga ttgttttatc
     1741 catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct cagatttgta
     1801 tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat aatagctttg
     1861 ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt tttttagttt
     1921 aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa aattcatttt
     1981 tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct tggagaaatt
     2041 ttccccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg aaaatagaat
     2101 gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat ctttatctgt
     2161 taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa agagtttctt
     2221 atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg gaccgagttt
     2281 tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt ttccgtatac
     2341 atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat ttgatttaac
     2401 ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg ttgcaatcgt
     2461 ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa ggcacataat
     2521 tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag tctaacagaa
     2581 gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt agttcgtaaa
     2641 aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa gaaagaaaag
     2701 ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc ggttgattgg
     2761 atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata atgcacttgc
     2821 ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg tcggacattt
     2881 agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa gaatagaaga
     2941 ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc tcgttgttgg
     3001 aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt atcggttaga
     3061 ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta ttgcagtggg
     3121 ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta gatttggtcc
     3181 gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat tgaatcctaa
     3241 catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca gcgcacttat
     3301 ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga attggacaca
     3361 aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt tctccactct
     3421 gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag catctatatc
     3481 aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat ataaaacaaa
     3541 gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa ggctgatatc
     3601 ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat tcaagcgaat
     3661 acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca ttttcatttt
     3721 atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa tgaagaaaac
     3781 agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga ttggaatcag
     3841 tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa ctgatcggat
     3901 tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca aaagcgtgaa
     3961 tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa gttcagaaaa
     4021 agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg agtatacaat
     4081 aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag ttgatttgat
     4141 tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca gttggacagt
     4201 tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag aaaacacgag
     4261 cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca cgagcagcga
     4321 aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca gcgaaagcag
     4381 tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga gtagcacagg
     4441 aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt tggtacctat
     4501 cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg tgctaaaaca
     4561 gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa cagataatag
     4621 aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta catggatggg
     4681 tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc atcgagtgaa
     4741 ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt gagtgaatat
     4801 ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc tatttttgtt
     4861 tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata agagcaagaa
     4921 taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac gaatctattt
     4981 gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag gagtatgtac
     5041 tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt tgaactattg
     5101 ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc taaactacac
     5161 cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa ggtatccaga
     5221 atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt cttctcagtt
     5281 tttactgagt aaagtttatt ctatactttt agagggaacg attggttata ttttactgga
     5341 ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa ttatataagt
     5401 ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa ctatcattta
     5461 tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat ccgatacttt
     5521 tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt atcaagtata
     5581 aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac gcgtagtata
     5641 gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa acaaggccac
     5701 tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat aacaaaagaa
     5761 gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc gatgaatcac
     5821 atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa cacctatttg
     5881 gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg gattgaatcg
     5941 tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat
     6001 tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg gacaaaaaag
     6061 aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat atttccagtg
     6121 atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc agagcttaat
     6181 acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt cagggatatt
     6241 tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg taaaacacag
     6301 gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa atatagtgca
     6361 tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa aaaggcatat
     6421 gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa tgattgggcg
     6481 cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat attggaaaaa
     6541 ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac attgcaaaaa
     6601 gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg agctcttgct
     6661 ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca gtcaaaagtt
     6721 ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta cgaatctcaa
     6781 tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta taactaatag
     6841 aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt
     6901 catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata tcatggacga
     6961 taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac aatttaatca
     7021 tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa atagtcgaaa
     7081 agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa ctagctagat
     7141 aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa acacagccga
     7201 cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc cgatacacga
     7261 attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa cgattgggaa
     7321 accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa aaccatggga
     7381 gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa
     7441 ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc attcgatcga
     7501 attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg ctcatggatt
     7561 aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc cttttatgaa
     7621 agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc tgacattttt
     7681 ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt tatccattct
     7741 gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata aaccatttat
     7801 tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata aaaaggagtg
     7861 aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt tgctgtaagt
     7921 acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc ggaatctcgg
     7981 gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa
     8041 acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga tcaagaaacg
     8101 accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac
     8161 gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga tgatatcggt
     8221 aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca gatgacagaa
     8281 atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt agtcactgag
     8341 cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat tgctacttat
     8401 ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca
     8461 gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa acaacttcat
     8521 caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata ttatgtcgat
     8581 caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg gaataacggg
     8641 atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt atttgttgat
     8701 tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata tgagtcagat
     8761 gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa gattttactt
     8821 tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga tattcctgtc
     8881 aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca actacgtttt
     8941 acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca gttagacatc
     9001 attggggatc aagagcatga agatttattt atgggccttt tgaaacaata ctattttgat
     9061 caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc agattcattg
     9121 cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa
     9181 ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc agggcatgga
     9241 aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt tttgaaagta
     9301 gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag tctactcaaa
     9361 gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac agagcaaaag
     9421 acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat ttttaatgag
     9481 acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc aaaaaagcaa
     9541 gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga tcaatcaagt
     9601 gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa
     9661 agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc aggtggattt
     9721 ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta aatgaatgaa
     9781 atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt tagtcactgt
     9841 atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa aacaatttga
     9901 cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa aaatcgaaca
     9961 aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata atatggatat
    10021 ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc atggaggcgc
    10081 atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat gttcagacac
    10141 gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac atcctacacc
    10201 agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag atcaagcaga
    10261 ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg gagaatattt
    10321 gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga ctttggagaa
    10381 caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg cacttactca
    10441 agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt actttgataa
    10501 taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac atgcggatcg
    10561 ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga aacaaatgga
    10621 agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag attatgcgga
    10681 gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac aagcgcaaga
    10741 aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc aaaagaggtt
    10801 gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag cttttcatgg
    10861 tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca ccttttattc
    10921 gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat tttataagtc
    10981 agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg gaggcaagaa
    11041 gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt ccgttgttat
    11101 tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta gcgaaaaaaa
    11161 gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt gtatgataaa
    11221 tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat gatcatctag
    11281 cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat cagataggta
    11341 caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat aatagaaaag
    11401 atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa cttttttatt
    11461 ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta aaatgaggcc
    11521 aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa ttttttagaa
    11581 aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac
    11641 tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc
    11701 gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa
    11761 aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc
    11821 tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac
    11881 gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt
    11941 gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa
    12001 aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt
    12061 ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt
    12121 gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat
    12181 ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag
    12241 ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa
    12301 tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg
    12361 aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat
    12421 tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat
    12481 ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc
    12541 ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa
    12601 tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta
    12661 gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc attttttccg
    12721 ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg
    12781 taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta
    12841 tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa
    12901 aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa
    12961 gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga
    13021 aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac
    13081 tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct tattataaag
    13141 attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga
    13201 cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag
    13261 aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt
    13321 atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg
    13381 gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg
    13441 acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg
    13501 aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt
    13561 ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc
    13621 cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag
    13681 caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg
    13741 agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt
    13801 ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa
    13861 gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt
    13921 gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc
    13981 cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc
    14041 gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat
    14101 ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg
    14161 gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt
    14221 gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt
    14281 ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta
    14341 gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa
    14401 gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta
    14461 tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc
    14521 ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat
    14581 ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa
    14641 tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt
    14701 cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg
    14761 tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt
    14821 caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa
    14881 cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat
    14941 ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa
    15001 tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct
    15061 agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag
    15121 taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg
    15181 gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat
    15241 tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga
    15301 atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca
    15361 agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga
    15421 tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt
    15481 tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag cacatgctat
    15541 gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt
    15601 agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt
    15661 atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc
    15721 gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca
    15781 tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg
    15841 ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt
    15901 tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt
    15961 tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag
    16021 ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg gttttttctc
    16081 caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat
    16141 tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg
    16201 gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca
    16261 aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg
    16321 ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat
    16381 tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag
    16441 taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag
    16501 attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata
    16561 ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc
    16621 aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg
    16681 aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga
    16741 taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag
    16801 atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact
    16861 atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc
    16921 atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac
    16981 tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat
    17041 cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg
    17101 aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc
    17161 ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta
    17221 tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac
    17281 aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt
    17341 gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc
    17401 gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt
    17461 acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac
    17521 acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata
    17581 aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt
    17641 gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt
    17701 tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag
    17761 ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga
    17821 atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga
    17881 cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa
    17941 tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa
    18001 attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc
    18061 tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc
    18121 ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga
    18181 atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct
    18241 ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga
    18301 tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt
    18361 tatctgtact tattacgata cacccgttca tgcattagga agttatttca tgtttgattg
    18421 gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag
    18481 tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct
    18541 atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa
    18601 agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac
    18661 gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga
    18721 aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat
    18781 tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt
    18841 ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta
    18901 tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg
    18961 agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa
    19021 aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa
    19081 gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga
    19141 aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa
    19201 attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag
    19261 tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac
    19321 ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct
    19381 tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac
    19441 tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg
    19501 ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa
    19561 aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc
    19621 ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag
    19681 gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag
    19741 atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg
    19801 gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt
    19861 ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga
    19921 tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg
    19981 gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga
    20041 ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg
    20101 aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt
    20161 ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc
    20221 catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag
    20281 ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt
    20341 ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata
    20401 tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc
    20461 atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa
    20521 tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag
    20581 agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact
    20641 ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt
    20701 aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc
    20761 attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa
    20821 gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta
    20881 tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa
    20941 gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg
    21001 gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg
    21061 aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca
    21121 aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat
    21181 aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat
    21241 gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa
    21301 taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat
    21361 gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa
    21421 caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga
    21481 atcgtccata tgagggtttc cacggctcta taatttctag gactgataga aaatcattct
    21541 atcggtccct tttgagcatg aaaaaacaaa acatacagaa gctccgttag aattgaatca
    21601 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca aactactact
    21661 tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca ttaagaatat
    21721 caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc ctttgtgtaa
    21781 acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc tcattcaact
    21841 taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct accactgtcg
    21901 tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact gctttatcgc
    21961 taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa tgacgacaat
    22021 tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt ctctgatccc
    22081 catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg aatttcgttc
    22141 actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata ctggaaaatt
    22201 actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta tgcaatttga
    22261 acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt cttatgcctc
    22321 tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc acattgtcaa
    22381 acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct ttgatcgaaa
    22441 cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc ttctgatcaa
    22501 acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg gttgggcgta
    22561 tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat tacgaatcgc
    22621 ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg acactttctt
    22681 taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta taaaaaaagc
    22741 ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt caaatgccaa
    22801 aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg gctttcgaag
    22861 ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct atgaatcaaa
    22921 aaacatctaa agtagaaaag gaattctacc ttagatgttc ttatctcact ctatcagtcc
    22981 tatttgacaa agagccgttt ccactcctta tgcagatttt tttaattctt gcactgcttg
    23041 ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag
    23101 attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga atgtcatagc
    23161 cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca
    23221 tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt
    23281 tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt tattcaactt
    23341 tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc attatccttg
    23401 tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg taatttgagc
    23461 gatatatcta catagataaa actccttttg cctatcaaat atttattgca acgaaaaaac
    23521 gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta aaatggttca
    23581 acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta tttgcacatt
    23641 tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg
    23701 atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct taataaaaat
    23761 tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa aattcattgg
    23821 tcgttgaatg ggctgaatag tagtgtataa aacaattttg tggagcgtga agcttttatg
    23881 gtagaggaaa atacgaataa agtgatgttg ataaaaagat tcctatcttg aatatgaatc
    23941 gagatgtctt gaaaatgaat gagtttgacc aatcattatc ctatcgaaat ttacagtttc
    24001 gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa tatgttctag gaagagggcg
    24061 gcacatctaa atttttaagt aacggacagt tcaaatatca agcattgaaa gaggctatcc
    24121 tactgtttga aggaatacga atatataaaa acgacaaaga ggaaaaagta gttcattaaa
    24181 tcccttcaag tgatgaaaga atgagatcta ataaaacctg atctttcatc tgttttttta
    24241 ataacgtaat aattttttta ttttttgtgc tttcgccgtt attgtagagt gatttagcat
    24301 tcttattttt aaaaataact gtgttatata ttaaaaaagg cataatagtt ataagctttt
    24361 ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa agcgttatta
    24421 ttttagtcta ttttcagggg gggaacctta tgataaaaaa actattttta ctatcaatat
    24481 taacgctagc aatggcaggt tgttctgact ctggcttagc aactgaactg atcacaaaat
    24541 caactgaagc cacgacagag gagttaaaat gtggaaacga tcacagtgac tacagaggct
    24601 taaatcgacg atgcatggga atattaggca gaagcaattg taagcatcga tgtagtaatc
    24661 gaagaaggtg tagaagaagc aacagaaaaa tcaaaagaaa gatcaaaaga agcaatctgt
    24721 gctgtgcatc gtatacagac gtcgaagatg cggtactttt aaaccacgtc aggagatgtt
    24781 catttatgtc tagggaagtt ggcgatgaac caaaactaga gtacgctttt agttttgcac
    24841 gtgaggatga agataaccct aaaacttacg acattttagc ttgatctaag atggttaaag
    24901 ctttttaatg aagtaagagg agatagaagg cttttctagt tatattaggt aagataaatt
    24961 cctttttgct ccaaatttgc taaacattct gctagataaa cttcatcgtg ttccggataa
    25021 atcggcacgt tgcgctctac agtaggtaaa tcaaaaaatg cttcgcatga tttgccacga
    25081 tacataggat ttttccactc tggtgctggg ctgtaaccac gtttttccat ttcatccatg
    25141 acaagctgat gatattggaa taatctataa ggagaatagt gaaaaacata atccacagta
    25201 gcatgtttct ttccccagcc atttcctctt aatgcacagc attcccgatg ttgtcctaaa
    25261 agctgttgac gaggaagctt tgaaatcaat ttttcatgcc ataatcacat agaaatgtct
    25321 cctttatata tagattgtta accttagacg ttaaatgaaa aaaccagcac caaggctgga
    25381 aaaaatcact tcagtttgct tgctgtttct gagcgaccaa ttaggtaatc aacggaaata
    25441 tcgtaaaagt cagcgatata gattaattca atgatgcttg gaatttcttc tccttcttcg
    25501 tacttacgat atttgaataa aggaatactt aacgcgctag aaacagctac ttgagataaa
    25561 gatcggccag ttcttaagaa attcagacga caacgtaaag tatccagtaa aatagcttct
    25621 tcgatactca tttaatcacc tttcattata gtagaatagt tataatgact atagaaagct
    25681 acacatgcaa tcaatagtac tcaatgtttt ttatttaaac gtatcaaata aagaatgacg
    25741 atcaataaag acaaaaccag tcctgataat tcttctataa aaacactatc gggttcaaag
    25801 tagttgtaat taaaaacttt ccacataaag tagatagccg gaagagtcaa aatagctaaa
    25861 taaaaccgtt ctttgttcca attcataaga taaacaatac ttgcgaccag tgcgaccgca
    25921 ggcacccaaa acaaaactct caccaacgta atcatgtaac ggactcctca acgattcata
    25981 taataaaaga taacatgtta ttgcatacat cacaaatgaa atgctaggaa aataaaagaa
    26041 gaatagatca atgaaaaatg aaaaagcaga acagttttct gctgataagc aggaactgtt
    26101 ccgctgaatg tgtcttatct atcgagaaaa aatcaatgga ttgatatcta agtaattcaa
    26161 tacatataaa aagaggacga tcgcattcac taaaaaggaa agaatgatga tcccatagcg
    26221 aatggaaaca tatttaacaa atacttttgt ttgaatacgg gcatatcttt ttcctttata
    26281 gctgagcatc atgacaagaa gcaaaagggt aatcacacct tgaaaaatca ttccaataaa
    26341 cagaattcct gaggtttgat aatttaattg gagaaaataa gctgctgccg caactaaatc
    26401 gaataaaatc gcaataaatg gcaaaataat cactcctaac attgagaata aataaaagaa
    26461 taacattttt tctaaaataa gcaaataaaa attaaagtaa caaaatagtt atgacacaag
    26521 taaaagaaat aagaataaca atattatttt acttatattt tgaaacttgt gagagtttgt
    26581 cattcgtgct atagttgaaa agtaacaaaa ataaatttta tttatgatgt acctttagct
    26641 cagttggtta gagcagacgg ctcataaccg tccggtcgta ggttcgagtc ctacaaggta
    26701 cataagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca
    26761 gcttcttatg tttttacttg gctgttctta ttactgcaga tgcaagcttt tgaataccgt
    26821 ttaaggaaga ggttgtgaaa tcagcgtttt gacctgagta ttagcttaac aaacggaaaa
    26881 atagttcctc atattttgcc gtttgttaag ctaataccga gggtcagctg attgaacacc
    26941 atttaagaaa gaggtcgtga gcctgccgtt tcactctgtg tcataaaaaa gccaatctac
    27001 tttgatagaa aaagcacgta ggactaaaac agttctgcgt gcttttttta ttgtgtgtta
    27061 aatatcgtaa atgaagatag atatttttat gattcgacga tagagacgtt gaaatctttc
    27121 aacagctcta aagcggattc tccgtgtttt ctactggaga ctaacattgg atcaacaaaa
    27181 aagtctgctt caggaaaagc actctgcagg cctatcagat tagagagtac acacaagttt
    27241 gtttcaatcc cgactacttc gatctctttt tgttcttctt tgctttcaaa taaagtttcc
    27301 tggatttcag ctaaagtttc aggtggaatc gtaaagtaat actttttaat cactcgttca
    27361 ttttcttgtg gagaaagaag agggatcaat tgcaaatctt ctcgttcttc ttctttgtcc
    27421 ttcagttcaa ctggtatatc ccgtgtgaag agaatatatt cattattttg gcgtgcttta
    27481 gctaatcttt gagcaatacg ttctgccaat tgatctgact ctggaatata aaattcgctg
    27541 tttggatcaa gaatatgatt ttgcatatca ataataacta acattttctt tcctcctaca
    27601 ttcatatcgt cattttattc tttttaatag aagtttttta aaatataagc aacaccagct
    27661 tcttcattcg tcagtgttac ttggtttgca atttctttta cttgtcgctg tgcattcccc
    27721 atcgcaatac tcaacccgct gtaattcaac atatcaatgt cgttttcttg atcgccaaaa
    27781 gccatgatgt cactcgattc gattccaagg taatccgcca gaaaaacaag tgaatgagct
    27841 ttagtgatac ctgtaggcgt gatttctaag aaatgcggat cagagaatac tgattggata
    27901 ccatctgctt gcagatctct ttgtagcttc tgtagttttt cttcactctt atctgttaat
    27961 gctaatttaa gatagctaag agaaggatct tccaacatat ttaaaatttg atcaaaagaa
    28021 tggttctgta aattgaaatg ttcataataa gggtcatctt tgactagatt tgattcatcg
    28081 aatgtcagct gatcatcttg ggtttctcgg ctagcaatga tggatatttt cgcttcttga
    28141 gcatagaaga aggcttttct cagtgtatcg ctatctattt ttgaacgtct gagaactttc
    28201 gccatttttc catccttcat ttcagcaatc agtgaaccat tggaagaaat caaatagcca
    28261 ccgattccta attcggaaaa aatatcttta gcagaaatgc tgttccttcc ggtagcgacg
    28321 gttacgattc cgccattttc atcaaatttc cgaagtgctt ggatattttc ttctggtatc
    28381 tttccatttc gatcaagcaa ggtaccgtct aaatcaatcg ctgctagttt cattttatct
    28441 tcctactttc tttttacata tctattttcc tgttacctta agattaaatg agaatttaaa
    28501 aaaatgaaac taacttgctt tgtcattatg aattttttca aaaaacattc ttaagaaaaa
    28561 ggtggctata tatagtagtt tttggtcaat acctttgaat aaaaagccat gaaaaacgaa
    28621 tagttagcgc ttaccgatgc gttttgtgat aaaaaaattt taaaatattc aatgaaaaca
    28681 aagcatatat tttgctttaa aaccaaatga ggaatattct agatgtagaa caggttagaa
    28741 aaagattaga aaaaggagtg attttgatgg agaagagata cggcgtagtt cctgtggtaa
    28801 ctgcttcaga cgaaaattat gctccttact tgagcgtgat gatcgcaact gcattagaaa
    28861 attgcaataa aacaagacgc ataaaatttt atgtgattga cgatggtcta tcagaatata
    28921 gtaaagaagg attggaagaa acggtaaata aatattcaag taatgcaagt atccaattcc
    28981 tgacagtgga aaaagatatc tacgaagact tcctagtcag tgaccatatc acaactaccg
    29041 cttacttaag aatttcttta ccaaacttat tagctaaaga ggattataag aaagtccttt
    29101 acttagattc agatgtgtta gtactagacg atatcgtaaa actatatgat gagccgttaa
    29161 atggaaaaac aatcggcgct attatcgatc caggccaagt gaaagctttg gaaagattag
    29221 gaatcgattc cgatgacttg tacttcaatt caggagtgat ggtgattgac attgatcaat
    29281 ggaataaaaa agaaattaca gaaaaaacta tccattacct cagtgaaaat ggcgatcgaa
    29341 tcatctatca cgaccaagat gctttgaatg ctgtacttta tgaagattgg gaacaacttc
    29401 atccaaaatg gaacatgcag acttcgctga tttttgaacg tcatccagca ccaaatgaaa
    29461 aatatgaacg tcagtataaa gaaggaaatg aaaaaccttc gatcgttcac tttacaggac
    29521 atgataaacc ttggaatacg ttgaaagatc atccgtatac caatttatac ttaaaaaaat
    29581 tagcacacag tactctaacg aaagtaggcg agatcaatga ataaaaaaga aatagcagta
    29641 gtcgcaagtt gtaacacaaa attcgtacca catttagctg cattatttgt ctcagtatta
    29701 gataattgca acccgtctaa atttgttcgg ttctatgtga ttgacgatga tatcgatttt
    29761 gaaagtaaac agcttttacg cttctcagtg aaaaacgcac gaatgaactc agatgtggaa
    29821 ttcttgaaaa tcaataaaga attctttaca aatgtggtaa tcagcgatcg tattccagaa
    29881 acagcttatt atcgtattgc gattccggaa ttgttccgcg gcacagaagt tgaacgtatc
    29941 ttatatatgg attgcgacat gatcgcttta caagatatct ctaaattatg gcgtcttgac
    30001 ttcggtgatt caatcgtagc agctgttgaa gacgctgggt tccatcaacg actggaaaaa
    30061 atgaaaattt cagctaaatc aatgcgttat ttcaactcag gtttgatgct gatcaatgtg
    30121 aaaaaatggt tagatgaaaa tattacgcaa aaagtattag atttcatcga acataatcca
    30181 gaaaaattac gattccatga ccaagatgca ttaaatgcta tcttacatga ccgttggtta
    30241 ccacttcatc cgcgttggaa tgcacaaggc tatatcatgg caaaagcaaa aaaacatcca
    30301 acagcagcag gtgaacgtga atacgaagaa actcgcaaca atccttacat tatccatttt
    30361 tcaggacatg tgaaaccgtg gagcaaggat tttgaagggc caacgaagaa atattacgaa
    30421 aaatatgcag ggatgacggc tttccgttgt gtagctaaat tccctaaata tccaaaatat
    30481 gcaaaagttc aaccaaaaac agatgtagtt gttaaataat agaagacaac aactgaagag
    30541 ggggagtagg aatggaaatt acaatgtcta aaaatgcagt agagacgtta attgaaaagg
    30601 ttggggaaaa tacaaaaatc gcactcgcgc ttatcaatga ttccgatcca tttctaaggg
    30661 acaaaggtgc ttttgcaaaa ggcagctttt ttcaaatcat tccttttgtt tcggaatttg
    30721 gagaatatgc gactaaaatc gagcatccat tattggacat ctatacatct aaattagagc
    30781 aaaattattt cggtaaacga ttgaacatgg acttcaacaa acagcttgac agcttttcat
    30841 tagaaaatga aatcgcagta ctcgattata atatcaaatt aaagaactgc ttcttcagct
    30901 aatagtttcg agaaagtcca aaaaggaggt atacattgag atactacttt gtggatgaag
    30961 aaaagaatgt aatagaaaca gatgaaaaaa aatataattg gctcgtactt gatagtgcaa
    31021 agcccgaaga gattgagcaa gcaatcgaag aattcgagct tcctgatgat attttcgtag
    31081 gaaccgaata tccggaagaa gtttctcgga tggagcgcct ttatgagacg aaactagagc
    31141 atccattttc tttggtagtg attaatctag atcagtcaga taatcgtatc gaaaaaaaat
    31201 taacgccaat atcttttgta ctttccgata atctcttgat tacttgtatc gatagaaaaa
    31261 ccgaatttgt ggatcggctg ctgacaaagc atggaaatca tttaaactcg tttgaaaaga
    31321 tcatcacgta tacattatta gatatttaca cccattacgt gaaagaacta agagagatga
    31381 aaaagcgaat tgacgctttg gataaagcag ctcgtaaaac aacggaaaat gaagaactgt
    31441 ataaacaggc agatcttgaa agagacatcg tatatatcga tcatacctta agagatcaac
    31501 gggaaacaat ggatcaatta tggaattctg aaactttcat caaacgttta gacgatgaac
    31561 agctactcta tgatgtccat ttgagacagc gacaaacaga aaaaatgatc gagatctatc
    31621 gagatttatt agagagtatt ggtgatctat tcaacgggat gatggacaat aatctaaatc
    31681 atatcatgaa atatctagat tcagctgcat taatcatttc cgttccggcg ttgttcgcag
    31741 ggatttgggg aatgaataca ggaggattac cgggtaaaag ttctgctcta ggctttatac
    31801 tcgtagtctt tggctctttg atagcagcag tagttttagg ttaccattta taccggaaag
    31861 attatacaaa ttaaagaatc tatttaaaac aggtatcaaa caaagaaatt tgttttggca
    31921 atacaattta aaaactagga ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg
    31981 aaacttgtac catacggaga aatttcctaa acaagatcag gatacaccag ctttacagca
    32041 gaatatgatt cctgttcctg attgtggaga agaatcctat gaaggcaata atcaactaga
    32101 aaaccgccgt gtactaatca ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc
    32161 atttgctaga gaaggggcag atatcgctct tcaattcttc ccaggagaag aaaaagatgc
    32221 agaggaagta gcaaaatacg tgaaagttgc aggacgtaag attgtcttac taccttttga
    32281 tcttcgtgac gaacaagcac ctaaagaaat catagataaa gccgtgagtg aacttggcgg
    32341 gctagatacg atggtactaa acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt
    32401 accacttgag caagtcaaag acacatttat gataaacatc atcgctatgt ttgctttagt
    32461 gaaagaagct gttccgcata tcccagcagg aggagcaatc gtaacaacaa cttctgttca
    32521 agcattcaat ccaagcgaac atttattaga ttacgcagca acaaaagctt caatcgctaa
    32581 ctttacgatc ggtttagcaa aacagcttgc acctaaagga atccgtgtaa atggtgtagc
    32641 acctggtcca atctggacac cattacaact agacgaagga caaccttctg attcgatccc
    32701 tgaatttggt cagcaaagtt tactagaacg tgcaggtcag ccaggagaat tagcaccagt
    32761 ttatgttttc ttagcatcga ataaagcaag ttatgttaca gcccaaattt acggagtaac
    32821 tggtggagaa gcgatcaact tataagaagt tgatcgtttc acccctatga ggaggtgcga
    32881 aaatgaataa aggtgtaaaa acgatcggag tgatcggaac attacttttg ctatctgtgc
    32941 ttttgtttgc gatactatta agtgcaacct atccattgcc ttttcagcta gagaagtttc
    33001 gattcttttc catcactaat tcatacgtgc aacaatatgt attttggctt gccgttgcat
    33061 ttgctatttt gacaattatc acgttacttg ttttcctgtt ttatccaaaa acaatcggaa
    33121 actttctact gaaacatggt gatggggagt tgacgttgga taaaaaagca attgaaggaa
    33181 tggttcgttc tcacttgcat gaagatgaat tcatccattc accaaaagta aatgtcaaag
    33241 caacgaaaaa caagatcgcc gtcaatgtaa aaggggagtt gaaacgaact tcagcactca
    33301 ttggtaaaac aggaacattg atgaaagaaa tcgaaaatga agtacaacac atattgggaa
    33361 caaaagagcc agtgaaagta tcggtgaaat acacaggctt tgaagaacca gctaaacgaa
    33421 gcaatgaccg caaacgagta gaataggggg ggaccaaaaa tgaaagaact attttcgaca
    33481 tacaagctcc ctatcatctt tggggtactt ggattgatat tagccgtttt atggatcacc
    33541 gtgggctttt tcaagacctt attgatcttg atcatggcag cgatcggcgt cgctatcggt
    33601 ttttatctga aacaaacgaa aatcttagat cactatttta aatcttagga ggaaacatcc
    33661 atgaataaca ataacaaaaa taataacaat aaaatgaaca accaaaaacc agtgtctgca
    33721 catccagacc caacgacacc aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca
    33781 gcaccagtat ctgcacatcc agatccagta gtccctaacg ttcaacaagg agtagaagag
    33841 aagaaacatg atgcagaaaa gcatgcacct catcatccag gaactccaga atctaaagtt
    33901 ccagaacaac atgatgcaat cgatccttct aaacctgtgg aagagcaaaa acatccttct
    33961 gaccatgcga aagcacaaga aaaacttagt tctgaccaca caaacggaca aggaaaactt
    34021 ggttctgatc atacaaataa ccaaggaaag catgattctg aacaggaaga ttcaatgcgc
    34081 ggtgagctga attatgaaga taaagttgtg caaaaaatca tcggaatcgc aattgaacaa
    34141 gtagacggtt tattatctgc aaacggtggt ttcttctcta atgtggcagg aaaacttgtg
    34201 aatacagata atgtaacggc tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt
    34261 gatatggatg tcattgtgga atatggcaaa gatatcgaaa aaatctttga agaaatgcaa
    34321 gaagtaatcg gcaaagaagt aaaaaatatg acacatttag aagtgatcga agtcaacgct
    34381 aacgtagtgg acatcaaaac aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac
    34441 aaagtaactg acgcagcgaa aaaaaccggt gaatttgctt cgaatcaaac agataaagta
    34501 aaatcagcag ctggaagcgg cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag
    34561 taataaggaa gtaaaacatt aggaggaacg aaacatggga tttatttggt cacttatcgt
    34621 cggaggaatt atcggagcaa ttgcaggagc aatcacgaat aaaggttctt cgatggggat
    34681 tattgcaaat gtcgtagcag gtttagttgg atcagcactt ggtcaagcat tattaggttc
    34741 ttggggacca agtattgcag gaatggcaat cgtgccatca ttgattggtg ccatcatttt
    34801 agtactaatc gtatcattct ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt
    34861 gaaaaagctg ttcttcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    34921 ttcatttggt tgggcatatt atcgaagatg caagcttttg aacactgttt atacggaata
    34981 agaggctgtg acaagacttt tgtcacagcc tcttatttat gcgggcaaat tcccaattag
    35041 atttttaaaa gtttttcctt aaatgtgtct ttattgatac agagaagctt ctttaataaa
    35101 acaatgcctc cctaaatttt cttgagatag gaaaggtgaa catacaaaat aatacgacca
    35161 ctttgaaaac ccagatgatc cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat
    35221 ggatcagaaa ctcaaagaga ttgaaaaaga caaggaagaa ataatatctc ttttttactc
    35281 ttgtctgttt tagccaataa tagctcgtaa agtaatctag acaaacaaat agaagtaaac
    35341 aaagtttcgt aagagcagca atcgtatcat gttgctctgc tagacgattg acgagcagga
    35401 aaaatgttgg taatggaata tagatcacga gtaatgaaag aaaaaagtaa acaataaatg
    35461 gcatactatt cctcctaatt gtgtgagttc gccctcattt taaaataacg atatttgttt
    35521 gtgaagcttt ttcgtgtcgt aacaaatgtt atctaatgta tttttaaata aataatcatt
    35581 attatcaaaa taatg
//
LOCUS       DACCDF010000026        34817 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCDF010000026 DACCDF010000000
VERSION     DACCDF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.026"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            598..1287
                     /locus_tag="IVK51_001199"
     CDS             598..1287
                     /locus_tag="IVK51_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430805.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IVK51_001200"
     CDS             1274..2482
                     /locus_tag="IVK51_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6430806.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IVK51_001201"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IVK51_001201"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP6430807.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVK51_001202"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="IVK51_001202"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP6430808.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="IVK51_001203"
     CDS             complement(6585..7280)
                     /locus_tag="IVK51_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP6430809.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLTPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVK51_001204"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="IVK51_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP6430810.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="IVK51_001205"
     CDS             complement(8479..8670)
                     /locus_tag="IVK51_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP6430811.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="IVK51_001206"
     CDS             complement(8691..9581)
                     /locus_tag="IVK51_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP6430812.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="IVK51_001207"
     CDS             complement(9568..10335)
                     /locus_tag="IVK51_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6430813.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVK51_001208"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="IVK51_001208"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP6430814.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVK51_001209"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="IVK51_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP6430815.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEVQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVK51_001210"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="IVK51_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP6430816.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14951..15289
                     /locus_tag="IVK51_001211"
     CDS             14951..15289
                     /locus_tag="IVK51_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430817.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="IVK51_001212"
     CDS             complement(15457..16215)
                     /locus_tag="IVK51_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6430818.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="IVK51_001213"
     CDS             complement(16233..17051)
                     /locus_tag="IVK51_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6430819.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVK51_001214"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="IVK51_001214"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP6430820.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IVK51_001215"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="IVK51_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP6430821.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18179..19513
                     /gene="dnaA"
                     /locus_tag="IVK51_001216"
     CDS             18179..19513
                     /gene="dnaA"
                     /locus_tag="IVK51_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP6430822.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19711..20841
                     /gene="dnaN"
                     /locus_tag="IVK51_001217"
     CDS             19711..20841
                     /gene="dnaN"
                     /locus_tag="IVK51_001217"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP6430823.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21070..21312
                     /gene="yaaA"
                     /locus_tag="IVK51_001218"
     CDS             21070..21312
                     /gene="yaaA"
                     /locus_tag="IVK51_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP6430824.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21299..22423
                     /gene="recF"
                     /locus_tag="IVK51_001219"
     CDS             21299..22423
                     /gene="recF"
                     /locus_tag="IVK51_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP6430825.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22420..24366
                     /gene="gyrB"
                     /locus_tag="IVK51_001220"
     CDS             22420..24366
                     /gene="gyrB"
                     /locus_tag="IVK51_001220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP6430826.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24386..26857
                     /gene="gyrA"
                     /locus_tag="IVK51_001221"
     CDS             24386..26857
                     /gene="gyrA"
                     /locus_tag="IVK51_001221"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP6430827.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDRAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27093..27392
                     /locus_tag="IVK51_001222"
     CDS             27093..27392
                     /locus_tag="IVK51_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP6430828.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27442..27954
                     /gene="ssb"
                     /locus_tag="IVK51_001223"
     CDS             27442..27954
                     /gene="ssb"
                     /locus_tag="IVK51_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6430829.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27980..28216
                     /gene="rpsR"
                     /locus_tag="IVK51_001224"
     CDS             27980..28216
                     /gene="rpsR"
                     /locus_tag="IVK51_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP6430830.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28359..30332
                     /locus_tag="IVK51_001225"
     CDS             28359..30332
                     /locus_tag="IVK51_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP6430831.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30338..30790
                     /gene="rplI"
                     /locus_tag="IVK51_001226"
     CDS             30338..30790
                     /gene="rplI"
                     /locus_tag="IVK51_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP6430832.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31064..32431
                     /gene="dnaB"
                     /locus_tag="IVK51_001227"
     CDS             31064..32431
                     /gene="dnaB"
                     /locus_tag="IVK51_001227"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP6430833.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNIIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33211..34503
                     /locus_tag="IVK51_001228"
     CDS             33211..34503
                     /locus_tag="IVK51_001228"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP6430834.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10606 a         6773 c         6880 g        10558 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggttgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgtggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tgtcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggactt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggaa tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaaagg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg cgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tgtatttttt atccacagac gcaagaggaa atctattggt ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa ctttttttga caactgtgtt tttctctttt ttctttttgt
    18061 cgattgtgtg gataattgta attaaaaaat ttttttcttc ggtttctgtg gaaaactatt
    18121 gaaaaaaatg ctaaattagt actagttatt cactttttaa tgaaggagga ggccttatat
    18181 ggtatccctc gatgctttat ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc
    18241 tgctagctat aatacatgga tcgaaacagc gaaccctcga acccttgatc aaaaccagct
    18301 cgttgttgag gttcctagca agattcataa agaatattgg gaaaaaaacc tggcgacaaa
    18361 aatcgttgaa gtcggttata tgttatcagg aaatgagatt atccctcgct ttattactgg
    18421 cgaagaagca gagcaagaag aagttataga agaaaaaaat ccaaaagttg tggcacctag
    18481 tccgctaaaa aaagccatgc tgaaccctaa atataccttt gatacgtttg tcattggtaa
    18541 aggaaaccag atggctcatg cagcagcgct cgttgtagca gaagatccag gttctattta
    18601 taacccgctc ttcttttatg gaggcgtggg acttggtaag actcacttga tgcatgcaat
    18661 tggtcatcaa atgctgcaaa gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac
    18721 ttttgctaat gatttcatca attcgatcca aaacaaaaca gcagaagagt tccgtcaaga
    18781 atatcgaaat gttgatttac tgctagtgga tgacattcaa ttttttgcag aaaaagaagc
    18841 tacgcaagaa gagtttttcc atactttcaa tgccttgtac aatgaaggga aacaaatcgt
    18901 actaaccagt gatcggttgc caaacgagat tccgaaactg caagaacgcc ttgtttctcg
    18961 ctttgcatgg gggctgtctg ttgatattac accgccggat cttgaaacaa gaacagcgat
    19021 tttacgtaaa aaagcggcgg ctgaacgtct agagatccca gatgacacac taagctacat
    19081 cgcaggacaa attgattcta acattcgtga attagaaggt gcgcttgttc gagtgcaagc
    19141 ttttgctgcg atgaatagtg aagatatttc gactagtcta gctgcagacg cactgaaaac
    19201 gttaaaatca ggaaaaggac atcctcaatt gtcaatcctg cagatccaag aagaagtggc
    19261 aaagtattat cacatccaat tgaaagatct caaaggaaag aaacgcgtca aatctattgt
    19321 tgttcctaga caaatcgcta tgtatttggc acgtgagctc acagataatt ctttaccgaa
    19381 aatcggagct gaattcggtg gcaaggatca tacaacggtt atccatgctc atgaaaaaat
    19441 ccagcagctg atggattcta gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt
    19501 tttactaaat taatttgtcg attctatcaa ctgaaaatag catgtggata aaagaaaaaa
    19561 acgttcaaaa gttatacaca ctttttccac agctgttttt tcgtttctct cttacgtttt
    19621 ttgagttttc cacagaatca acatgcccta ttacttttat tattcttttt aactaataat
    19681 aaataaacaa acattcaaag gagatacatc atgaaagtta ctttaaaccg agctagcttt
    19741 atgcaggaat tgcaaactgt tcaacgagct atttcaagca aaaccacgat ccctattttg
    19801 acaggtgtaa aaatcacact gacacaagaa ggtttgactt tgacggggag caacgctgat
    19861 atatcaattg aaactttttt gtctgttgaa aacgaaaaag caaatatgca aatcgaatct
    19921 actggttcca ttgttttaca agcacgtttc tttagcgaaa tcattcggag acttcctgaa
    19981 gaaacattta ctttagaagt tttagaaaat aaacaagtag cgatcacttc tggaaaagcg
    20041 aattttatcg taaatggatt agatgcagat aactatcctc atcttcctgt tgtcgaaagc
    20101 cataaccaga tgaaattacc tgtacacgta ttgactaaac taatcaacga aacagttttt
    20161 gctgtctcac aacatgagag tcgtccaatc ttgacaggtg tgcatttcat tttatctgat
    20221 aattctttat tagctgtagc tactgattct caccgtctaa gtcaacgcgt gattccagta
    20281 gaacaagcgg ctgatcattt tgatattgtt attcctggaa aaagtttgat cgaattatct
    20341 cgctcattaa caaatgaaga agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg
    20401 ttcaaaacag aaacgatgta tttctattcc cgtttgctag aaggaaacta tccagatacc
    20461 aatcgtttga ttccttcaag ttttaataca gaggttgaat tttctgttcc tagcttttta
    20521 gcggcgatcg aacgggcttc tttactttct catgaaggcc gtaacaacat cgttcgttta
    20581 tctattcgtc cagatgcggt tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa
    20641 gaaagcttga gttatacagc aagcagtggc gatccattag atatttcttt caatccggat
    20701 tatatgaaag cagcgttgcg tgcatttgga gatatgagca tcaaagtgaa atttatctct
    20761 gctattcgtc catttacatt ggaaccaacg gaagatggcg ttcaatttat tcagctgatt
    20821 actcctgtac gtacaaatta acaatgattt tcaatgttct tgaaaattta attgaaaaga
    20881 aatagaaaaa ataaaatttt aaacagaagg ccctaaaatc tcttttaagg ctttctgttt
    20941 atttttgttt aaatcacctt ttttagatta aatcgtctta tatggaaaaa tatatggata
    21001 aatttgtttt attgcgaaaa aaaaggtata atagagtata cttttaataa tgaaaatgag
    21061 ggatgggcat tgaaacaaca agtcattcta aatacggaat tcatgacgtt gggccaaatg
    21121 ttgaaagaag tcacagttat cggtagtggc ggacaagcaa aatggtattt ggcagaaaac
    21181 actgtgttag tcgatggcga accagaaaat cgtcgtggac gcaaacttta tcccggcatg
    21241 atggttgaag ttccagaagt aggaactttt tttatggtaa aaaaaggaaa cgacgataat
    21301 gaggctgaat aagctgtatt taaaaaatta tcgaaattac gaagagctga acatcgaatt
    21361 ttccaagaac ctcgtcatct ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga
    21421 aagtatctac gtattagcta tgacgcgaag tcatcggaca agtagtgagc aggaattgat
    21481 tggctggact gacgatcagg cgatgattca aggagagatc acaaaaggtt catccactct
    21541 tccattagaa atcttattat ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca
    21601 gaaaaagtta agtacttacg tgggacaaat gaatgtcatt ttatttgcac cagaagatct
    21661 ttctttagta aaaggcagcc cgcagatcag gcggaagttt ttagatatgg aattaggcca
    21721 gatcaatcca atctatcttt atgatcttgt ccaataccaa gcaattttga aacaaagaaa
    21781 ccagtacttg aagcaattga atgaaaaaaa acaaactgat ttgctttatt tggatatttt
    21841 gtccgaacaa ctagcagcgt ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa
    21901 gcgattggag ttttgggcta attctttgca tcagcaaatc acccatcaaa aagagcagct
    21961 ggaaatcgag tatctcaccg cagtcgattc tttagaaaca catacgcaag agcaaatcca
    22021 agaacaattt ctcgcacttt tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac
    22081 gacagttggt cctcatcgtg acgatctttc cttttttatt aatcagaaaa atgtgcagac
    22141 ttatggatcg caaggacagc aaagaacaac tgcactaagt gtcaaattag cagaaatcga
    22201 tttgattaaa gaagagacag gggaatatcc aatccttttg ttagatgatg tgatgagcga
    22261 gctggatgat aatcgtcaac tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt
    22321 cttaacaaca acgacacttg accatgtaaa aaataaaatg actgttgaac ctgagatttt
    22381 ttatgttcaa caagggaaac taaaaggaga agaaactgaa tgacagaaga aagaagttta
    22441 gtagaacgcg ctaaagagta tgatgccagt cagattcaag tacttgaagg acttgaagcc
    22501 gttcgtaaac gacctggcat gtatatcggt tcgactagtt ctgagggtct gcatcacctt
    22561 gtttgggaaa ttgtcgataa ttcgatcgat gaagtattag caggttttgc aacgaaaatc
    22621 catgtgatta ttgaaaaaga caacagtatc accgttattg acgacggtcg ggggatacct
    22681 gtagatatcc aggcaaaaac tggacgtcct gcagttgaaa cggtctttac tgttttacat
    22741 gctggaggaa aattcggcgg tggtgggtac aaagtttctg gtggacttca cggagtagga
    22801 tcttctgttg taaacgcgct ttctacacag cttgatgtca aagtatacaa agaaggaaat
    22861 gtgtactatc aagaatatcg tcgtggagct gtagtggacg acttaaagat tatcgaacaa
    22921 acggatcgcc atggaacaac ggttcacttt acgccagacc ctgaaatttt tacagagaca
    22981 actgagtttg atttttcaaa attggctacc cggatccgtg aacttgcttt cttgaaccgc
    23041 gggatgcgta tatcgattga agacaaacga gaagaagaac cagtcatcaa tgaataccat
    23101 tatgacggcg ggatcaaaag ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt
    23161 ccagaacctg tatatttgga aggtgaacag caagatatcg cagttgaagt ctctatgcaa
    23221 tatacagacg gctaccattc caatatcatg agttttgcca acaatatcca tacgtatgaa
    23281 ggtggaacac atgaatcagg attcaagact gctttaactc gtgtgataaa tgattatgcc
    23341 cgaaaacaaa agctgatgaa agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa
    23401 ggattgacag ctgttatttc tatcaagcat cctgaccctc aattcgaagg acaaacaaaa
    23461 acaaaattgg gcaattccga agtacggaca gtcactgatc ggttatttag cgaacatttc
    23521 atgaaattct tgctggaaaa cccaagtgtg ggccggcaaa ttgttgaaaa aggattacta
    23581 gcttccagag ctcgtctagc agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct
    23641 ttagagatta gtaatctgcc aggaaaatta gcggactgct ctagcaacga tcctgaaaaa
    23701 tgcgaattgt tcatcgtcga aggagattcg gccggcggat cagctaagca aggtcgtaat
    23761 cgagagtttc aagccatctt gcctattcgt gggaaaatct tgaatgttga aaaagcaagc
    23821 atggacaaga tcttggcaaa tgaagagatc cggtctttat ttactgctat gggtacaggg
    23881 tttggagaag attttgatgt atcgaaagct cgctaccaca aattagttat catgactgat
    23941 gccgatgtcg acggcgcgca tatccgtaca ttgctgctga ctctttttta tcgttacatg
    24001 cgtcctattg ttgaagctgg atacgtctat attgctcagc cacctttata cggggtaaaa
    24061 caagggaaga atattacgta tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa
    24121 gtcgttgctt cacttcctgc ctcaccaaaa ccaagcgttc agcgttataa aggacttgga
    24181 gaaatggatg atcatcaact atgggaaaca actatggacc caagcaatcg gatgatggcc
    24241 cgcgtcagtg tggacgatgc tattgcagca gatcagatat ttgaaatgtt gatgggggat
    24301 cgagtagaac cccgtcgtgc attcatcgag gaaaatgcac actatgtgaa gaacttagat
    24361 atttaatcat gaaggaggaa aaagaatgag tgaagaaatc agagaaaaca tccatgatgt
    24421 caatctaacc agcgaaatga aagactcctt catcgattat gcgatgagtg ttattgtcgc
    24481 acgtgcactt cctgatgtca gagatggtct gaaacccgtt catcgccgta ttttatacgg
    24541 catgaacgaa ttaggtgtaa caccggataa agcccataaa aaatctgcca gaatcgtcgg
    24601 agatgtcatg ggtaaatacc atcctcatgg agaccgtgcg atctatgagt caatggtacg
    24661 gatggcacag ccattcagct accgttatat gctagtggat ggtcatggaa actttggttc
    24721 tgttgacggt gacggtgccg ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc
    24781 tacagaaatg ctgcgtgata tcaataagaa cactgttgat ttccaaagca actatgatga
    24841 tacagaaaaa gaaccagttg tactacctgc tcgcttcccg aatcttttag tgaacggaac
    24901 aacggggatc gccgtaggta tggccacgaa tatcccacct cataacttgt ctgaggtagt
    24961 agcggctatc gatttgctga tggataatcc tgatgtgacg accaatgaat tgatggaagt
    25021 attacctgga ccagattttc caacaggtgg tttggttatg gggaaatcag gaatccgaag
    25081 agcgtatgaa acaggtaagg gttctattac cgtacgtgca aaagttgaga ttactgagat
    25141 gccaaatggt aaagaacgga tcatcgtgac agaattgcct tatatggtca ataaagcaaa
    25201 attgatcgaa cggatttctg aactgcacag agacaagcgt atcgaaggga tcacagattt
    25261 gcgggacgaa tcttcccgtg aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag
    25321 cgcatccgtt atcttgaata acttatataa attgacatcc ttgcaaaatt catttggatt
    25381 caatatgttg gcgatcgaaa aaggtgtgcc gaaagttttg agcttgaaac aaatccttga
    25441 aaactatatc gaacatcaac gcgaagtcat cacacgacgg acggtctttg aaaaagaaaa
    25501 agcagaagca agagcacaca tcttagaagg tttgcgtatt gctttggatc atatcgatga
    25561 aatcattgcg attatccgcg ggtctaaatc agatgacgag gcaaaagcaa caatgatcga
    25621 acggttcgat ttatccgatc gccagtctca agctatttta gatatgcgtt tacgccgatt
    25681 gaccggtttg gaacgggaaa aaatcgaaaa tgaataccaa gaactcttaa aactgattga
    25741 agatttaact gacattctgg caagaccaga acgtgttaca gaaatcatca agaatgaatt
    25801 agcagacttg aatcaacgat ttggtgataa acgacgcaca gaattactag tcggtgaggt
    25861 cttaagtctg gaagatgaag atttaatcga agaagaagaa atcgttatta cattgacaaa
    25921 caatggttac atcaaacgtt tggcaaataa tgaattcaga gctcaacgaa ggggtggacg
    25981 tggtgtccaa ggaatgggta tccacaatga cgacttcgtc aaaaatctgg tttcttgttc
    26041 tacccatgat acattgttat tctttacgaa taacggaaaa gtgtatcggg caaaaggata
    26101 cgaaattcca gaatacgggc gtacagccaa aggaatccca atcatcaatt tactaggtat
    26161 cgattcgagt gaaagcatcc aggcaatcat tgcggtgacg ggagaagcag aagaaggcca
    26221 ctatctcttc tttacgacgc gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc
    26281 taacatccga agtaatggtc tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa
    26341 tgtgctactg acagatggtc aatctaatat cattattgga acacataacg gctactctgt
    26401 cactttcaaa gaagaagcag tccgcgatat gggacgtaca gcttccgggg tcagagggat
    26461 acgcctgcgt gaggaagatt atgtcgttgg ggcagcctta atgaaggaaa atcaagaagt
    26521 attagtgatc acagaaaaag gttacggaaa acggacaaaa gtatcggaat atccagtgaa
    26581 aggccgcgga ggtaaaggga tcaaaacggc gaacatcact gaaaaaaatg gtccactagc
    26641 tggattgacc actgtttctg gagatgaaga catcatgctg attacagata aaggtgtcat
    26701 tatccgtttc aacgtatcga ctgtttctca aactggacgt tcaacattag gcgtacgttt
    26761 gatgaagatg gaagctgata cgaaagttgt cacgatggca gcagtcgaac cagaagctgc
    26821 agaacaaaca gttgaagaac aaggatcaga agaataacca tcaagactaa gtgtgtaagc
    26881 tagcaagaat aaccgattaa aaaatctggt tattcctgct ggcttttttg attagttgaa
    26941 tttctcttaa aatattcgaa actgttcatt ctgtattgct tttgaccaaa atagagtgta
    27001 taattagagc ttgtgagtag tctaattaat tagataactc tccttgctct taggaaccta
    27061 agagccgaaa gtccacaggg aggtgaatat caatggaaaa cacgaaatat gaaatcatgt
    27121 acattattcg tccaaacatt gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg
    27181 caatcttgaa agataacgga gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt
    27241 tcgcttatga aatgaacggt taccgtgaag gtatctatca catcgttaaa gtatcttctc
    27301 catcatcagc aaatgctgtc aatgaatttg accgtcttgc taaaatcaac gacgatatca
    27361 ttcgtcacat gattgttaaa gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa
    27421 cgatgagagg agatgactat tttgattaat aatgttgtat tagttggacg tctgacgaaa
    27481 gatccagatt tgcgttacac agcaagtggt acggcagtcg caacatttac tttggctgtg
    27541 aatcgtaatt ttacaaacca aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt
    27601 tggcgcaaat cagcagaaac tttagcaaac tatgctcgta aaggaacact tttaggtgtt
    27661 actggacgta tccaaactcg ttcttatgat aatcagcaag gacaacgtgt atacgtgaca
    27721 gaagtagtag ctgacaactt ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa
    27781 caaagcggcg gattccaaag ttctggtcaa tcagctggcg gatttggcgg aaacaataac
    27841 tctaatcaaa catctcaatc atccaacggt atgcctgatt tcgaccgtga tacttctgac
    27901 ccattcggct caagttcaac gatcgacatt tcagacgatg atttaccatt ctaaacgaat
    27961 ttgataggag ggaaatagaa tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt
    28021 agactacatt gctgctaacc atattgaata tatcgattat aaagatatcg aattactaaa
    28081 aagatttatt tcagaacgcg gtaaaatctt gccacgtcgt gtaactggta caggcgctaa
    28141 aaaccaacgt aaaattactg ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt
    28201 cgttagcgaa gactaataaa tcctaggaat tgaatgactg atccttttga tcagctattc
    28261 aattcctttt tttctgtaaa aataacctct attcgtttag tcgctcgaat gacgaaagtt
    28321 atggtaaaat caaattagtc atatatggga ggaaaattat ggaaaataaa aacaatcatt
    28381 tatccccgaa acctctcttc cttatcctgt ttatattaat cgaagtattg gtagtcttaa
    28441 ttttaccatt tcgttttgtt agcgcttcgg tcattctatt gatgaattta tggggtgttc
    28501 gctattggtt gaaaatgaaa cgttggctta ttttaaataa acgtgaaaaa atccagcagg
    28561 cttcgactgt agctgaaacc aatctagctt atgtctcaga agtcatgcct gtaggagtga
    28621 ttgcttatca gccatcaagc caaaaaatcg aatggctcaa tccattagca ttgaaaatca
    28681 aagatcaaag tggattaaca gaggaagaat tgctagatac tttttttact gcaaaagaaa
    28741 aaaagaaaaa ccaagtctat ctagcagata aaacatttca gtttgatatg gatacagtaa
    28801 agggtgttgt ttattttaat gatattaccc aaaaccagtt gttaaaactc catcagacga
    28861 acagtcagcc ggtgattggt attttatcta ttgataacta tgcagatgcc attgataaat
    28921 tagatgacaa agagatttct tatttgaact ctttcgtcac tacactagtt tctgactgga
    28981 tgaatgaata ccatgtcttt ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac
    29041 agatgatcga tctgaaaaag atgatggata aaaaatttga tttgctggat cgctttcgag
    29101 cagaagctga aaaacagagc tcgcctatca cccttagtat ggggattgct tatggctcaa
    29161 gaaccctcga tgaaatcggt ggagaagctc agaataattt agatgttgct ttagtccgtg
    29221 gaggcgacca agttgtcgtg aaagaggtaa aagaaggagc aaaacctgtt tattatgggg
    29281 ggaaatctgc taagaatgtc aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt
    29341 tgcgtcggat ttttgatgaa acaggcgatg tgtttatcat ggggcacaag tttccggata
    29401 tggatgcttt aggctctgct tttggtgtca gttgtctggc acgcttcaac cagaaaaaag
    29461 cttacatcgt tatcaatgag aacgagatca ttccagatgt agaacggtgt cttgaagaaa
    29521 tccataagca tcctgaatta gaaatgcaac taatctctcc agaacgtgcc atagagctga
    29581 aaaaagaaga ggacctcctc gtgatggtgg attaccataa accttctcta tcgatctctc
    29641 agggcctata tgagcaattt gataagattg tgatcattga tcaccatcgt cgtggggaag
    29701 agtttccaag taaacctctg ctgacttata ttgaatcttc agcttcttca gcttcagagc
    29761 tagtttctga gctgatccaa taccaaagca gcaaaacaaa tcgcttagat cgttttgagg
    29821 ccacattatt gcttgcaggg atggttgtag atacaaatag tttcaatgtc cgtacaactg
    29881 cacgaacttt tgacgttgcc agctatttaa ggtcttgtgg agcagacgca tccttagtac
    29941 aatatttatt gagctcggat ttagcgacat acttggagat cagcaatttg attgctggaa
    30001 gcgactatgt gacttctgat attgttgtgg cggcagggac agacgaaaaa gagtataata
    30061 gtgttatagc agcaaaaaca gcagatacat tgctttcgat gatcaatatc aatgcagctt
    30121 tcgtcattac aaagcggaca gacggcttga ttgggatcag tgccagaagt accggcacga
    30181 tcaacgtcca aatcatcatg gaatccttag gtggcggagg acacttcaca aacgcagcta
    30241 ctcaactgga aaatacgaca gttgaggaaa caaaacagaa gctatttgct gcaatcaaag
    30301 aaaatatgaa tcaaatatat gataaggagt aatccaaatg aaagtaattt ttttacaaga
    30361 tgtaaaagga aaaggtaaaa aaggggacat caaagaagtt ccagcaggtt atgcacaaaa
    30421 ttatctaatc aaaaataact tggcaaaaga agcaacgaaa agcagtatca gtcagttagc
    30481 aggacaaaag aaagcacaag aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt
    30541 aaaagaattc ttggaaaaag aagatactgt agtcgaattg aaggcgaaag caggggaaga
    30601 cagccgtttg tttggttcga tcccttcaaa acaaattgct gaagggttaa acaaacaata
    30661 caaaatcaaa ttagataaac gaaaaatcga attagaaaat ccgatccgtt cactaggcta
    30721 cacaaacgta cctgttaagc ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt
    30781 acaagaataa caaatcttga tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa
    30841 gacaaaccga aatttatctg agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg
    30901 atttcagatt gatttcggtt tcttttttgg gaacagcaaa atagcataaa gaaaacgagt
    30961 ataataaaag agaactattt ttttattaca ctttcggtac gaatgatgac ttttctctca
    31021 ttttcttgta aaatgaagca attgaagaca aggagcgata cgtatgaatg aagtgtggca
    31081 ggatcgaatc ccaccgcaaa atattgaagc tgaacaagcg gttttaggct ctgtttttct
    31141 tgacgcagaa acgatcattg atgccatgga gtacatagag ccaaaagact tttatcgtcg
    31201 tggccatcaa attatttttg agacaatgat cgagttaaat gatcgtaatg aagcgattga
    31261 cgtggttact gtcaaagacc ggctggaaca agccaatttg ttagaagatg ccggtggttt
    31321 aagttatctc tcagacttgg cattagcagt tccgacagca gcaaatatcg tctactatgc
    31381 gaaaatcgtt gaagaaaaat cattgctgcg tacattgatc cagacagcaa caggaatcgt
    31441 tacgaaagga ttcgagcaag gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag
    31501 tatcttggag gtttcggaaa aaagaaatcg aagcggcttt ttatctattg ctgaagtact
    31561 gaactccacg attgaaaata tcgatcgact agctcaaaac aatgaagaaa tcacaggtct
    31621 accaactggc tatcaaggtc tggataaaat gacagcaggt ttccagcccg aagaactaat
    31681 cattcttgct gcacgtccag cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa
    31741 tgtaggaaca aaaacagatc gttctgttgc gatatttagt ttggaaatgg gggcagaatc
    31801 tcttgtaaat cggatgcttt gtgcagaagg ttcgattgag gccagccatt tacgtacagg
    31861 tcagctttcg gaagaagaat ggtcgaatct gatcgttgcg atggggagtc tttctcgagc
    31921 aaatattttt atcgatgata caccagggat caaaatctct gaaatccgtg cgaaatgtcg
    31981 gaaactagcg caagaaaaag gcaatctagg cttgatattg atcgattatt tgcagttgat
    32041 tgaaggtaac ggtaaagaga accgtcaaca agaagtatca gatatctctc gccaactgaa
    32101 aaaactggcg aaagagctga aagtacctgt cattgctctt tcacagttat ctcgtggagt
    32161 agaacagcga caagacaaac gaccagtgct aagtgatatt cgtgaatccg gatcaattga
    32221 acaagatgcg gacatcgtag ccttcttgta tcgagatgat tattatgaaa gagaaggcgg
    32281 agatgacgat gatggaaacg agccaaaaaa tgacaacatc atcgaagtca tcatcgagaa
    32341 gaaccgaagc ggtgcacgag gaacagttga actactgttt atcaaagaat acaataagtt
    32401 ttcgtcgctt tcgccaagag aagaatttta aagaagaggt tgtgagcctt gagcaatccc
    32461 gagagctctc gtaagaagat gcaaggagta accgcaggaa cgactagagt acttaattta
    32521 aacagatctc caaagggatt gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac
    32581 ggtgttcaat cagctgaccc ttggtattag ctcagcaaac gtcaaaatat gaggaactat
    32641 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tcttacgacc tcttttcctt
    32701 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatcatcaa acatgagaag
    32761 cagttttttg aaattgctcc ttgtgagtca gagttaaacg gcaggctcac aacctctttc
    32821 ttaaacggtg ttcaaaagct tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga
    32881 gaagctattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaacctc
    32941 tgattcacgg tgttccaaaa gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt
    33001 tgagaagcaa ttttcgtaca ttccttctta tttcttgaag ctgaccactt ctgttacaac
    33061 ctctttttta ataaccattc taaaaagata aagttcgttt tttaagcgtt tttataaaat
    33121 aaaaataaaa atcattcgat tttatttgaa atatgtatct attattgata caatggttca
    33181 ggattaaata atatagaacg aggtgttcac atgtcatctg tagtagtagt aggtacgcaa
    33241 tggggcgatg aagggaaagg aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt
    33301 gcacggtacc aaggtggcga caatgcaggg cacacaatta agtttaacgg tgtgacttat
    33361 aaactgcatt tgattccttc tgggattttt tataaagaca agatcagtgt gatcggaaac
    33421 ggcgttgtag tcaatcctaa atcattggtt aaagaattag cttatttaca tgaaaaaggt
    33481 gtagaaacaa gtaatttgcg tatttctgac cgcgcgcacg taatcttgcc ttatcatatc
    33541 aaattggacc aattacaaga agatgctaag ggcgaaaata agattggtac aacgattaaa
    33601 ggaatcggac ctgcctatat ggataaagca gctcgtgtag gtatccgtat tgcagactta
    33661 ttagataaag aaatttttga agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa
    33721 tttgtaaaaa tgtttgattc agaaccactt gattttgacg atatttttga agaatattat
    33781 gaatacggac aacaaatcaa acaatacgtc acagatactt ccgttatctt gaatgatgct
    33841 ttggatgcag gcaaacgcgt gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac
    33901 cagggaactt atccttttgt tacttcttct aacccagtag ccggtggggt aacaatcgga
    33961 agcggtgtag ggccttcaaa aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca
    34021 cgtgtcggtg atggaccatt tccaactgaa ttatttgatg aaacaggaga acaaatccgc
    34081 gaagtaggac gagaatacgg tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat
    34141 tctgtagtta tgcgccattc taaacgagtt tcaggtatta ccaacctatc tttaaactcg
    34201 atcgatgtat taagtggatt ggaaacagta aaaatttgta cggcttatga attagatggc
    34261 gaactaatct accactatcc agcaagcttg aaagagttga accgctgcaa accaatctat
    34321 gaagaattgc caggctggtc agaagacatt acaggatgca aaacacttgc agaactgcca
    34381 gaaaacgcac gtaactatgt acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca
    34441 ttttctgtcg gacctgatag aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc
    34501 taaggataaa aacgacgact gaatcaggac gttcttacca gagcgagtcg aagtgtttat
    34561 ctatacgata ataataactt gaagaggtag tgaaagagac gtttagcctt attgccgata
    34621 ggctgtctct tgaacactgt ttattcagaa taagggactg tgacaaaaac cgtgtcacag
    34681 tccctttttc cgaacaaacg gtgttcaaaa gcttgcatct acggcactaa gcccaatcag
    34741 atgaaaaata tggagtgcta tttttatctg attgttctta ctgcttgatg cagagcagct
    34801 ttttcacaac ctctcca
//
LOCUS       DACCDF010000027        34784 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCDF010000027 DACCDF010000000
VERSION     DACCDF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.027"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(233..1081)
                     /locus_tag="IVK51_001229"
     CDS             complement(233..1081)
                     /locus_tag="IVK51_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6430835.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK51_001230"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IVK51_001230"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP6430836.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IVK51_001231"
     CDS             complement(3582..3761)
                     /locus_tag="IVK51_001231"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP6430837.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK51_001232"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IVK51_001232"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6430838.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IVK51_001233"
     CDS             5909..8188
                     /locus_tag="IVK51_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6430839.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IVK51_001234"
     CDS             complement(8281..8928)
                     /locus_tag="IVK51_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430840.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IVK51_001235"
     CDS             complement(9107..9826)
                     /locus_tag="IVK51_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6430841.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IVK51_001236"
     CDS             complement(9957..11108)
                     /locus_tag="IVK51_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP6430842.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKGNAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IVK51_001237"
     CDS             complement(11325..12065)
                     /locus_tag="IVK51_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6430843.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK51_001238"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IVK51_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP6430844.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IVK51_001239"
     CDS             complement(12449..13042)
                     /locus_tag="IVK51_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6430845.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IVK51_001240"
     CDS             complement(13026..13673)
                     /locus_tag="IVK51_001240"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP6430846.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK51_001241"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IVK51_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP6430847.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK51_001242"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IVK51_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP6430848.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IVK51_001243"
     CDS             complement(15119..15646)
                     /locus_tag="IVK51_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP6430849.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IVK51_001244"
     CDS             complement(15826..16611)
                     /locus_tag="IVK51_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP6430850.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IVK51_001245"
     CDS             complement(16604..17473)
                     /locus_tag="IVK51_001245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP6430851.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IVK51_001246"
     CDS             complement(17474..18853)
                     /locus_tag="IVK51_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP6430852.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK51_001247"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IVK51_001247"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6430853.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK51_001248"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IVK51_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP6430854.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK51_001249"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IVK51_001249"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP6430855.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK51_001250"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IVK51_001250"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP6430856.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK51_001251"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IVK51_001251"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP6430857.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IVK51_001252"
     CDS             complement(22811..23035)
                     /locus_tag="IVK51_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6430858.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IVK51_001253"
     CDS             complement(23088..24050)
                     /locus_tag="IVK51_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP6430859.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IVK51_001254"
     CDS             complement(24060..24500)
                     /locus_tag="IVK51_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6430860.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IVK51_001255"
     CDS             24806..25771
                     /locus_tag="IVK51_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP6430861.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IVK51_001256"
     CDS             complement(25828..26058)
                     /locus_tag="IVK51_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP6430862.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IVK51_001257"
     CDS             complement(26144..27031)
                     /locus_tag="IVK51_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP6430863.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IVK51_001258"
     CDS             complement(27120..28343)
                     /locus_tag="IVK51_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6430864.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IVK51_001259"
     CDS             complement(28360..29439)
                     /locus_tag="IVK51_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP6430865.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVK51_001260"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IVK51_001260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP6430866.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IVK51_001261"
     CDS             complement(31297..31563)
                     /locus_tag="IVK51_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP6430867.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IVK51_001262"
     CDS             32042..34276
                     /locus_tag="IVK51_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6430868.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IVK51_001263"
     CDS             34424..34744
                     /locus_tag="IVK51_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP6430869.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9842 a         7510 c         6060 g        11372 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcatttccct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt cagatatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggtagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaa
//
LOCUS       DACCDF010000028        32194 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCDF010000028 DACCDF010000000
VERSION     DACCDF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.028"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            161..490
                     /locus_tag="IVK51_001264"
     CDS             161..490
                     /locus_tag="IVK51_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430870.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            477..818
                     /locus_tag="IVK51_001265"
     CDS             477..818
                     /locus_tag="IVK51_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP6430871.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            932..1171
                     /locus_tag="IVK51_001266"
     CDS             932..1171
                     /locus_tag="IVK51_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430872.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1238..1477
                     /locus_tag="IVK51_001267"
     CDS             1238..1477
                     /locus_tag="IVK51_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430873.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            1604..2293
                     /locus_tag="IVK51_001268"
     CDS             1604..2293
                     /locus_tag="IVK51_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP6430874.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            2299..3465
                     /locus_tag="IVK51_001269"
     CDS             2299..3465
                     /locus_tag="IVK51_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6430875.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            3547..4218
                     /locus_tag="IVK51_001270"
     CDS             3547..4218
                     /locus_tag="IVK51_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP6430876.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            4271..6247
                     /locus_tag="IVK51_001271"
     CDS             4271..6247
                     /locus_tag="IVK51_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6430877.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            6260..7012
                     /locus_tag="IVK51_001272"
     CDS             6260..7012
                     /locus_tag="IVK51_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6430878.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            7028..7789
                     /locus_tag="IVK51_001273"
     CDS             7028..7789
                     /locus_tag="IVK51_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430879.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            7802..8062
                     /locus_tag="IVK51_001274"
     CDS             7802..8062
                     /locus_tag="IVK51_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430880.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            8059..10149
                     /locus_tag="IVK51_001275"
     CDS             8059..10149
                     /locus_tag="IVK51_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6430881.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(10185..12242)
                     /locus_tag="IVK51_001276"
     CDS             complement(10185..12242)
                     /locus_tag="IVK51_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP6430882.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(12387..12578)
                     /locus_tag="IVK51_001277"
     CDS             complement(12387..12578)
                     /locus_tag="IVK51_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430883.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            12766..13221
                     /locus_tag="IVK51_001278"
     CDS             12766..13221
                     /locus_tag="IVK51_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430884.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            13218..13460
                     /locus_tag="IVK51_001279"
     CDS             13218..13460
                     /locus_tag="IVK51_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430885.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            13478..13780
                     /locus_tag="IVK51_001280"
     CDS             13478..13780
                     /locus_tag="IVK51_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430886.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            13849..15903
                     /locus_tag="IVK51_001281"
     CDS             13849..15903
                     /locus_tag="IVK51_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430887.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIPKWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQH
                     LAEQKVAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            15903..18515
                     /locus_tag="IVK51_001282"
     CDS             15903..18515
                     /locus_tag="IVK51_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP6430888.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLKTLKEIQQIYPQ
                     IPESKGSFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     RQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            18512..20890
                     /locus_tag="IVK51_001283"
     CDS             18512..20890
                     /locus_tag="IVK51_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430889.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            20951..21493
                     /locus_tag="IVK51_001284"
     CDS             20951..21493
                     /locus_tag="IVK51_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430890.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            21571..21903
                     /locus_tag="IVK51_001285"
     CDS             21571..21903
                     /locus_tag="IVK51_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430891.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            21904..22497
                     /locus_tag="IVK51_001286"
     CDS             21904..22497
                     /locus_tag="IVK51_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430892.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            22519..24543
                     /locus_tag="IVK51_001287"
     CDS             22519..24543
                     /locus_tag="IVK51_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP6430893.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            24543..25742
                     /locus_tag="IVK51_001288"
     CDS             24543..25742
                     /locus_tag="IVK51_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6430894.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            25758..26402
                     /locus_tag="IVK51_001289"
     CDS             25758..26402
                     /locus_tag="IVK51_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430895.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            26413..27219
                     /locus_tag="IVK51_001290"
     CDS             26413..27219
                     /locus_tag="IVK51_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430896.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            27242..27471
                     /locus_tag="IVK51_001291"
                     /pseudo
     CDS             27242..27471
                     /locus_tag="IVK51_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27630..28565
                     /locus_tag="IVK51_001292"
     CDS             27630..28565
                     /locus_tag="IVK51_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP6430897.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            28609..28878
                     /locus_tag="IVK51_001293"
     CDS             28609..28878
                     /locus_tag="IVK51_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430898.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            29154..29429
                     /locus_tag="IVK51_001294"
     CDS             29154..29429
                     /locus_tag="IVK51_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430899.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            29471..29893
                     /locus_tag="IVK51_001295"
     CDS             29471..29893
                     /locus_tag="IVK51_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430900.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            29913..31505
                     /locus_tag="IVK51_001296"
     CDS             29913..31505
                     /locus_tag="IVK51_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430901.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31526..31861
                     /locus_tag="IVK51_001297"
     CDS             31526..31861
                     /locus_tag="IVK51_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430902.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            32007..>32194
                     /locus_tag="IVK51_001298"
     CDS             32007..>32194
                     /locus_tag="IVK51_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430903.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        11590 a         4964 c         6567 g         9073 t
ORIGIN      
        1 attggatcaa tcgagcattt accgctgtct actagggttt ctcgtttcgg acgccataaa
       61 cgagtcgatt tattttatat atttttgagt gaacgaaaaa cgataaagat attcgttatg
      121 aagtaaaact aatttagtag atagcaaagg tggtagagga atgattcaaa aatttgcgaa
      181 aatgagtaaa actgcatgga ttgaaaagct tgaaaatgac ttgagtgtca tgcgattagc
      241 tccaaagaaa ttcaaacgag agattgcaga aaaagaagca attttgtcgg tattgaatgc
      301 ggtagacgat aaatgtccag taaaaattag tttttcagat atagcaacaa ttatcgaagg
      361 tatttattat ggtgaagttg atgaagagat atcttctaca aaagatttat tagatttttt
      421 ggataaaaac aaagatacct ttatagtact aactgattat gaggaggtta caacaaatga
      481 aaaattatga tccgaatata cgattgggca cacatacgat taaggtttct ttccaacgtt
      541 gggattacaa aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt
      601 tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg
      661 gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt acggatgata
      721 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      781 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      841 agataaaggc ttagaaggag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      901 gagtaaggaa tttatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      961 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
     1021 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
     1081 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
     1141 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
     1201 tttatttttg caaaaaacaa gaaaggtagg aaaactcatg aattatgaag aaatcgaaaa
     1261 cagaaaaaaa gtgtcgaaag aaatggaaga aaagttgcta aaaatgatga aacaaaaaca
     1321 ccttaaaaga ttatctgtca tgcaatatat caatgatatg aagataacag gaaaagagaa
     1381 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg aggcgaactt atgtaaaaac
     1441 aggttccaat tgccaactat tattagaagt ttcttgacga ttatgatatg aaaatgatca
     1501 attatgatgt ggcattgtta ttgaataaaa agaatagtaa aatttaacat ggtttatctt
     1561 ggttagatgt aggtaaatta attcatatat agaaggtaga tagatgaata aaaacaataa
     1621 aactttattt aatccttacg tgggcgtaat cattgttttt cttcttttta gttattttgc
     1681 ttatcaaggt tatcaaaagg acaaaatcgc tgaaaaagta gatggtgtaa caaaaacaat
     1741 ccatattcag gaacaggcta aaacctatca agataattta accagtttaa cggatatttc
     1801 taagagacaa ctgattacta atcaaaaaca ggcattagaa aaggatctca aaggtgaaat
     1861 tgctggaaaa cttgtttttt cttctacagg atttattctc cctgtttttc aaggagcgaa
     1921 tagcgacacg cttagtttag gagtagcttc tacttattat ctagatactg aaatgggaaa
     1981 tggaaattat attcttgctg gttataacgt agatacacca agcgttttat tatccgatat
     2041 gactgattta tctgaaggag aagaaattga tctaatcgat gctcattaca agtatcagta
     2101 tgaagtctcc aaaagaggaa aaatttctga ttacagccag gtagttcaat ctaaggaaaa
     2161 caattccttt ttatccttgc caaaagcagg taaaaaacca ctactcacac tgttagcctt
     2221 tcaagcaaat aatatgaaaa agtgcgatgt gatccagtgt gaattagtca atgtagaaaa
     2281 aatcaataaa taagggaagt gagtcacatt caagaacttg tagaaaaatt gatcaataaa
     2341 atgagtgcca tttttctgtt actgttggta tccattctat atttaatatg gacggtgggg
     2401 ttaaatcctc ctacgatatt atttatttat gataatgctg cacagttagt gacgtttttc
     2461 gtaggctgtt tgtttctttt gaatctgtac aaggtaaaga gaaacgatct attcttcatt
     2521 gcagcaggca ttctttttta ccttttcttt tcttttttta gaagctaccg aggagatact
     2581 ttctatggtg atctgatcgt tccgacaatt attgctatta ttttgattgt aaaatggacg
     2641 aaatttactg taattgatcg tagcttgtat cttgtagtat tctatgtctt tttactcgta
     2701 acggtttatc gagtatttac tgagatacca gtaccagaag gacaaagtat ttggaatcca
     2761 tcgaacaaat tatcagatat ttggatcaat acaaatacga taggtagttc tttaatgaca
     2821 ttagcccttc ttatcacagg atttgctagt tcatttggaa aatggtacat gcgattgcta
     2881 tcattaccag cgttgattct tgctggggca acgatttggg tttgccaatc gaaagcagca
     2941 ttatatgcgc ttgttatctt cattatctta gaaattttac ctaagaaggt ttttcgaaat
     3001 acacacttgt ccttttggat gtatgcagca gtttcggttc tagctttacc gatttcttat
     3061 tttgctgcaa tgtcagacaa agttcactta tttacaggac gagaagaaat ttggcgcaaa
     3121 ttctatgaaa cattaggcga aaaaacagga caaatatttg tagggatgaa acctttcttt
     3181 ttccatagag gaaatcagat tctaggtaat cataatacgt atcacgcact cgtcaatcac
     3241 tatggattga ttggttttgt tctatcgatt attcttttgc ttagtttctt atggttgctc
     3301 gtaagaaaac gtgagctgac aaatggacaa ctaacattct tgtgggcttt tttagcgatc
     3361 atgtgtcaat cgttcatgga agaaacatta acgacaatca gttgggtgcc aattgtattc
     3421 ttaatgttag caatggctac acaccaatat aaaaccacca gataaatgaa atagaatagt
     3481 aataagagaa aacacttttt cgttgtgaaa aagtgttttt tgttttgtct gaaaggaggc
     3541 agatggatga aaaaatggtt atttggtttt ttaggagtcg ctttaatcgt cgtttgcagt
     3601 gtgtttggct atgttagcta ccaaaaacat gaaggagagg tgtttaagca aaatatagaa
     3661 aaaaagatgc ccgtagatca aatcaatgca cacgcaaaga gttataagga ggatgcaacg
     3721 aacgtcaata atgatatgtc tttggggcaa atgttgtcca tacaaaaaga agcaatcgaa
     3781 atgggcgtca ataaacaagt atttgcacaa attcagattc ctgcacttgg tttagcattg
     3841 ccaattttta aaggggcaaa tcaatacact ttgagtcttg gagcggctac ttatttttat
     3901 gaagatgccg aaatgggtaa aggaaactat gtcctagcag gtcataacat ggaaatgcca
     3961 ggtgttttat tttctgatat tcaaaagttg tcattagggg aggtgatgga cttagttagt
     4021 aacgatggcg tctatcgtta caaggtaacg cgtaaattta tcgtacctga gtactttaaa
     4081 ttaatcgatg gcgttcctga agagaactct ttcttgtctt taccaaagaa aggagagaaa
     4141 cccttattaa cgttgtttac ttgtgtttat acgtcacaag gaaaagaaag atacgtggta
     4201 cagggagagc tacaataaca agaatttaat caacgcgagt cgttgaaata tgaaatgagg
     4261 agagtaaaaa atggcaataa atcggtatgt aaaaaaagct ggaatgattg ttggaatgtt
     4321 aggaatcatt gcaggctgct ttcaggggta tcgagcttat gcagaagatg tcacgcaaaa
     4381 aacgccacca gaaagggtca atatcacggt tcataaatta atgtacgatc aaagtacgca
     4441 attgaacgtt gatattgacg gaatcaaaaa tgatggttat acgcacgatc aatatcctac
     4501 aggtgtaacg aaatacaaca aggcagatta tggtgatgtt gagtttacgt taggaaatat
     4561 tacggatcaa gtattaccga ctgaagattc tgatctaacc aacgcgaaag tggacgaaat
     4621 cgttaaagat gtagaagaca agggatctaa cagtgagtac gttaagaatg caaccaataa
     4681 aattacttca gctgtagatg aaaatggtga gatcactttt gctgatcaac cagcctatgt
     4741 caattccaaa gggaatgtat atgtggttta tgaaagtaaa tccgctgctg gattagtcac
     4801 acaaaaagca aaaccaatgg ttgtgattgc gccaatgaca gacaacacag gatcaggatt
     4861 tttaaaagat attcatttgt atccaaagaa cattgtttct aagttgagtt ttgaattaac
     4921 gaaatttggt gacgatggca cagctcaaag caaacaaaca cctttaaaag gggcaaaatt
     4981 tgagttatac aagggcgaac ctggaaaagg aacaaaatta ggagagcttg tatcggatga
     5041 tcaaggaaag ctgactgcaa ctgatttgac gttaggaaaa tactactttg tggaagttcc
     5101 ttcagaagtt gtagttggat cagatcaaga accaacagca gaccaatatt tgttaggcgc
     5161 agacgctcgc aacgatgcgc acaataagtt aacctttgaa attacgaatg acggcgtgac
     5221 aagtgattta aaagcttcct acgtgaacta taaagcacca gtgatcgata aaacagtgac
     5281 aaatggaaca ggagaagaac attctttcca aattggtgac gcagtgaatt atcaaggtac
     5341 aattcatatc cctacagata tcgcaggtgg aacagatggg attacagtaa atggtgtaaa
     5401 aagtgaaact tctccttaca gtgtatttaa atggggcgac acagcaggta aaggactttc
     5461 ttatgttgct gaaaaagcag atattaaagt aacaaataat gatggttcag tcgtattaaa
     5521 agaaaatact gactataaaa tcgaaaattc tgatagtggt tttgtgatcg attttatcgt
     5581 aaataatgga caagtaagtg atacagttgc gaatttacat ggtcaagatc taaaaatgac
     5641 ttataacatg tttgtgaatg atagtgctga aatcgcaaat ccattaacaa atagcgtgga
     5701 tttcgtttat aacaataatc catttaatca ggaagaacac cacgaaaaaa cgaaagctga
     5761 cgttgtgact tatggcgcaa aattcttaaa agtcgatagc ggcctatttg gaacaggtat
     5821 caaagcaaca ccgcttgaag gcgcagaatt tgcagctaag aatgcagaag gtaagtacta
     5881 cggtggactt actgatacag ataaagatgg tgtcaaagaa gcagtttggg tagacgatgt
     5941 agcaaatgca gcaatcctaa aatcagataa agagggtcat tttgaaatca ctggtttaac
     6001 tgaaggagat tactcactgg aagaaacaaa agctcctgaa aactatcaaa aattaacaaa
     6061 agagatctct ttcaaagtag acaaagattc atacaaagaa gaaaaccgaa tcacgatcaa
     6121 aaataatcaa aaagcttccg taccaatgac tggtagcaac ggtttccaat cttacgtatt
     6181 gatctcttgc ttgttgcttg gggcaggcgc aatttctgca gttgtttact ttaagaagaa
     6241 agcctagaag gatcagctaa tgattatcag acaccctaag aaaaagcgaa tcatgggtaa
     6301 atggatcatt gctttctggt tactttcagc tgttggtgtc ttacttttga tgcctgctga
     6361 agcgagcgtg gcgaaatacc aacaaaatca gcagattgcc gcaatcgatc gaacaggaac
     6421 ggcagcagaa acggattctt ctttggatgt tgctaagatt gagttaggcg atcctgttgg
     6481 tattcttaca attccaagta tttctttaaa actaccaatt tatgacggaa cttccgacaa
     6541 aattttagag aatggtgtag ggattacgga aggaactggc gatattacag gaggaaacgg
     6601 taaaaatcct ttaatcgcag ggcacagtgg actttataaa gataatcttt tcgatgattt
     6661 gccttcagtt aaaaagggag aaaagtttta tatcaaagtt gatggtgagc agcacgctta
     6721 tcaaatcgat cggattgagg aagtgcaaaa agacgaacta caaagaaact ttgtcactta
     6781 cttagaacca aatcccaatg aagatcgagt aacgctaatg acatgtacgc ctaaggggat
     6841 caacacacac cgtttcctag tgtatgggaa gcgagtaacc tttacaaaat cagaattaaa
     6901 agatgaagaa aataagaaac aaaaattatc ttggaaatgg cttttaggaa gtactgtttt
     6961 tctttcagtc atgatcattg gttcgctatt tgtttataaa aagaaaaaat aaaggagggt
     7021 aaccaacatg agaaatagaa aatggctatt tgtttgtgca gccgctcttt tttctttcac
     7081 tgcctacaca cagttgacgt ttgcagacag acaagaaagc acggatagca cagaacaaac
     7141 aatccaaacg tcagatagca gcacgattca aagcagcgat gaaaccgtcg attcgtcaac
     7201 agttgattct tcagaagaac ctaaaattgc tgaagacgat caaatagtca aacagaccat
     7261 tcacgttcaa aaaattattt cttccgaaca attagacaac caggggcaaa agcttgaaaa
     7321 acaagaagga gtaaatggcg cgaagttttc tgtttatgat gtatcggaca ttttacaaaa
     7381 gatggatgtt aaggatctca ctactgacca aattgaatcc caattaaaag atcgagtgaa
     7441 aaaactatcc agtgatcaac tgaagctaat ttctaatgga gaaacaaaga caattgatca
     7501 acaaacaggt gtctttgaat tctcggtaga agtacaggca aatcagaaac aagcttatta
     7561 tattgtcaat gaatcaagtc ctgaaaacat ttctaatagc gaggatattt tgctattaac
     7621 acctgtttct gataaaaatg gtgaattctt aaaggatgtg tggatttatc ctaaatcaga
     7681 agcttcgcaa cctaaagaag aagtaaaaaa aatcgtatct actggcgtaa agaaaaactt
     7741 ctttgagaat tgttgggatt ttgttactca cttgttttct agagattaag gaagaaataa
     7801 aatgaaactg atcaaattat tattattcaa tttttcttgt ttaaccgcag tctttttgta
     7861 ctgcggttta ttattttcta aactatctat tttgccaagc taccaactat acctaatgag
     7921 gattttaaac ataccagctg cttccatgcc gctagatatt atggaaaagc tcattaggaa
     7981 tccattattt actacgatca atttgatcgt tattgtcgga atttcactag tcatttataa
     8041 aaaagagggg atgaagggat gaattttaaa cactcgtgga caagacggtt aaaatatgtt
     8101 tttcccataa tgatgatgtt aggtacattg tttgggttaa aaacagccaa tgtctttgct
     8161 gaaaaagtta ttgtcgatcc gtcaaatcca atccagaacg taaagaataa ttttattatt
     8221 ccaaaagatg ttcctaatgc aaaaacaaat attactgtaa acggagcgtc aaaagtttac
     8281 aaatatcatg ttgatgccaa caaaggtggt tttacagacg actacgtagg attagtggag
     8341 ggaaacaaaa atatacgcta tccttctttc caaaaagaat acggtgaaac aaaccttgtt
     8401 ttagaaggcg taacaaccaa caccaaagtc aatattgact atggaaaaat cggtacgtat
     8461 aacgggaaaa aagtcaatat caagctagtt ctatctaata ttcacttata ctcggatacg
     8521 ttgccgtgga atctactaga taacaactat acaaaaactc attttcgaga cgatggctat
     8581 aaaaatacga atggtgcaat gagtaaatct aaaaaaagaa cagtactttg gatctcagac
     8641 aatcttttct caggaattgt ttaccattca acacaaatga atgtgcagct agtagccaca
     8701 tatgaggacg gtagccccgt tcaattttca ggagatacat ttatcagttt taactctttg
     8761 aaccctgcag gaggaaaaag cacggatctt aaaggagaat atgcccacta tgacaaaatg
     8821 aatacgacag attggtatgt cagaaaagat acggttcttt cagaatttaa atctttctac
     8881 aacaatctga acgtggtagg tggccaccca ggaggaagct ctaagctgac acaagcggat
     8941 aatgatttca ataatttgca tgataaatta ggagatccga agtttggtca aggtactgtg
     9001 tcctttaaaa tatctgaagc aaatccgacg tttgtgattg gttcttctaa tgttcaaacc
     9061 tggtttactt tatcttctgc gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca
     9121 ggggttgata aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg agacacgatt
     9181 caatatcgaa tcaaacagaa ggtgaatcga ttaggagtgg atttactagc ggtttacgat
     9241 cgatttgagt taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa
     9301 agtggtacaa ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa aacaaaacac
     9361 caggtaaaat atgcagcgaa agctgataca ttaaaaaatc gaatgaagta taacggcgaa
     9421 acttacgaat tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc
     9481 aaaaatcagg gaacgtcgat cattaataaa gtagaaaagg atacaaatat cattaccgtt
     9541 tatttcccta aaattccagt gaaagaagta caacaaaatg gaaaagatgt aaacggacga
     9601 aatgacggta agaagggtac gccgacagct cctttaaacg caggatctga agtacaatat
     9661 ctagtcacac aaaaatggca cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa
     9721 tttagtattc aagatccaat tgaagctcgt ttgacatata aagaaggttc tgcgcaagta
     9781 atagataaga gtaaaggaaa agatattact tctgaaggca cactaaccta tgatagcaat
     9841 tcaagaacgc tgaagtggga agcttccgct gacttcttaa ataacaatct tttagatgga
     9901 cgagaaattc agctgatatt tacagctaaa actccattac aatcagaaaa aaatattgat
     9961 aaccaagccg tagtagcagt agataatgtt tctaataaaa caaatgttgt gacaattggg
    10021 gttgatccta acttgccaca agttattgtt cctaaaacag gttctacaca tttagtaacg
    10081 attttagcgg tcagcttact attacttgtg ttagcaactt ttggctacgc agttctgaag
    10141 ttcaattaaa caaaaaaaca aggctgaaaa tcagccttgt ttttctattt gaaaatggga
    10201 ttggcactaa taaatacgtt gtctgaagaa agtgatccgt ctgcagaaac ttcataagca
    10261 tgggataaac caacgatact ttgattgttg taaagcaagt ttgcgttatg agcagcgcta
    10321 tctttccaca taaccatagc atttactgct cctgtatctg aaaggatctc gtcaacgtaa
    10381 aggtagaaac tattgatatt ttctccttta gcaccgtagc agcctaagct ttctaagaga
    10441 tcttggaaag taccgccgcc aggtcggtaa tgactgaagc ttacttgtaa ttcttttgca
    10501 cgaatatctg cagcttgttg aacgatatca ttgaattgca ttggggcaac gccattttgt
    10561 tgacggtagc tatttaacca agcaagttct ttttgacgca catagttgtt taaatcactt
    10621 agtgagatat ttttgtattt gaaagtgatc gttcgatttt ggtcaacatt attgattaca
    10681 cgttgtccat ttttatcaac ggtaacagat gaatcgtcat ttctacacaa gaagtagttt
    10741 tgatcggtca tatttttcca agaagtagag aaactatctc cctcgtaaac cgtttgtgtt
    10801 tcagtagata aaacagcgtt tgtgtcaacg tttatatggt tcacagtgac gttgtattga
    10861 tgagtatagg caaaattgat tccttgatta cttgtaacgt tgtttaaagt cacagtgttg
    10921 ctagagcttt ctctaggatc aagaacaaaa ttagtgaaca taggtgcttc aattgtgatg
    10981 ctttgtccat aaacgacggg ataactctta ctgtaattat agagttcttg gatatttgga
    11041 tcagtatccg agactttatt tggacgagct ttcacttcaa ctagaaagac ttcatcatag
    11101 taaaaagtaa atgtggtatt tgtgttaatc gtttgtgttt gtgtttcatt cccatttact
    11161 tgatactgtg gtgcttcaaa gttagtcaag tcggttctag cttttgcagt gtaacttgta
    11221 ccagctttta ctgagacatg ttcttctttt agcagcgttc cagtttgtcg attcatatga
    11281 cggacagtta ccgcatattc tgaaggttta ggtttgtgtt gtaacccttt aaatgcagaa
    11341 tcaagactat ttttagcatt atttacttgg gtttgagaag ctttagaatc gtctagtact
    11401 tttttagcat tggataaagc agtctgaaaa ttattccaag atccttctgt aaaatctcct
    11461 ttagcagttg attttacttg gttgtacaag gcttgtaatt ccgttttgtt cacagtagga
    11521 gctggttttt tctttaatcc cttgtaagca gaatctaatg tgtttttggc actatccact
    11581 tgtttttgag tcgcttttga gtcatttaac acttttttag cattgtttaa agcggtttgg
    11641 aatgcgttcc aggtattgtc ggtatagtca ccttttttag ttcccttaac gctcgtataa
    11701 agagcttcta aagctgattt attcactttg ggtttagagg tgtcagcacc ataccaacca
    11761 ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt ccaacttgca
    11821 gtgtagtgat gcgaaccaga ggtaacacct ggaaggaaaa ggcggtagat cggtttatta
    11881 tttttttcgg cagaacgcca ggcaggccct tcataagtcc aaccgtaatt tttggtcaac
    11941 catttcactt catcccaact agctgtgtaa tggtgatcac gaaggccttt attgtataaa
    12001 cgatagacaa ggtggccacc gctagtcggt gcttcccaag cggggccttc ataattaccc
    12061 cacttaattt tcactaggtg atcacgttct tttgtgctag aggtgtaaaa atgttcatgg
    12121 ttatttggat tataaaggcg atacatgacg ttagttgaag cagcgtcagc agatccagca
    12181 agagcaactg tggcagcaaa tgttccaata gctataacag tttttgggtt taataatttc
    12241 atgttttcaa ctccctatta aatgattaaa tattttgttt acattcataa tataccacgg
    12301 tataccgagg tagtcaacgg tataccgtgg aaattttgaa ataaaaaagc cgccctatga
    12361 agagtgtaag gatggctgtt tatatgctat gaagaaatgg attcggcgta tttggttagt
    12421 ttggcagcat tatcaaatcg cattttttca atcgcacttt ttttctttat taaatccgca
    12481 attgttgatt ctgcgactga agaatgtttg ctaatttcat atgctgtctt gccagtttca
    12541 aatagtaacc attcgatttt ctctttatca gcgtacattg caatgctccc tcttgatttt
    12601 ttctttatta tagctctttt ttgtaataaa tcacaatatc aagaggaaaa gtttctatag
    12661 aaaggtggga aattatgagt atccgttccc cgtgtatcaa aatgaggttt gttgctactt
    12721 tcgttattta atatactaaa ataaaacaac gaaagaagga atagaatgaa aaagttaata
    12781 atggtattta cttcgttgat tgccatgatt gttattttag gtggatgtgg tagtgaaaaa
    12841 aataagcaag atagtcaaga taatggcgtg ttaaaaccag gaacagtgtg gaaagaagaa
    12901 gtaggcggac ttgttcataa tttaaaaatt atagatgata caacatggga atattccgaa
    12961 agtgtttggc atcctgaaca agtacaaata acagttgaga agctaaaaga ctataaagga
    13021 ttagaaagat ataaggtaat cgattcaggt ggagtagatt tattcatagg taaaacagcc
    13081 ttaatagttc cctataacaa agacggttta gaaacgttta aatttattcc aacaaacgaa
    13141 aaagaacatc gagataaaag tgaaatatta aaagaataca gtactaaatc tggatacaag
    13201 ttacaaaaga ctagcgagtg attgctagtc tttttattta tcctgaagga ggaatcgcag
    13261 tggcaaaagc taaaacgcca tttgaaaaag ccgcgtctaa aatcttagaa cgtcgcggtg
    13321 tatctgaaac acagtggaaa caagaagtcc ttgaaaaagc acaatttgac ctattacggg
    13381 gaaaagacaa ggacttagaa ggatatatct atcaacgtga acgtgaaaag cttgttttcg
    13441 atgaaattat taaacactaa aaggagcgaa caattttatg tttgaatcaa ttacaatgaa
    13501 ttttttaaca caaccgaaag tagtagctgc ggttgatatt tttagagcat tacaaggtgc
    13561 agcaactagt ttaaaaaaca atctgatgat ttttgcggct gtagctgccg ttgcctccat
    13621 tgttcttggg gggattttct tcttctttgg ccgaagagga gctgaaaacg ggaagatgca
    13681 cgtaggttat gtattgatgg gggtaggagt tatattagct gccgcctcta ttgtggtttc
    13741 gttatttgga atgtttggac aaaccccaca gggactataa aaaataaata gaaggagtaa
    13801 aaagtacgtg gctacttttt actccttttt tatgaaagga gaatactcat gtactttacg
    13861 atttcaggca ataccaaaaa agataaagag ttaataaaaa aagcaacgaa gaaagtggga
    13921 aagcgtactg cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag
    13981 ctacatgtgt taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc
    14041 acgttacatg ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggccca
    14101 ctggattatc aaatttcaga aggtttttat cctacattga tggatttgat ccgccttgat
    14161 gtgtttaagg aaacggatga aaccatgagt gtggaagagt cttatcgaaa cgaaaaactg
    14221 ctacaagttt tagaagaaga gttagagagc gaccaaagaa aagtgagtca agtgagtgaa
    14281 gtcgaagtaa atgatgaagt accatgggaa cgaccaatct ttccaaagaa ggagtcaaaa
    14341 gaaaaagaga cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt
    14401 gaggaagaga acgatgtaat gggtcaattg gaggtagatc cctttgaaga aacaatggag
    14461 gatgaagaga cggccgaagt agaagttgat ctagttgaag ctaatgaaga gccaacatgg
    14521 actgaagggg attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaac
    14581 gaaacagaga tacaaaaggc aactgtattt gattatattt tggatcaata tccgccagag
    14641 catgaatgga tgaaagaaaa aaataaatta tttttaaatg agctttattc gaaatatcaa
    14701 ttaccccaaa cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa
    14761 ggcacacttt tagaacagtt agagtctgtt caatcaacaa attggcatga gcttgcacaa
    14821 gaagatttat tagccacttt tgaagaacgt caacaagaaa cagatcaaga aataaatgag
    14881 tatgcgacgg aacagtcatc taattgggag agaacaaaag aggaactaaa taaagaagaa
    14941 caacgagtga ttgaagaaga agtacaaaaa attaaagagc gatacgaaaa gaaacgcgac
    15001 aatgcgtatt ccttgtgtat agagaaaatt aataacttta ctaaaactaa tcaagaagct
    15061 ttatcgaaag aaaaagaaaa cttgttaaaa gaacgtataa aagaacgaaa agaagagttc
    15121 taccgaggtt tacgtacaga taagctaaag attagcaaag aattaaatga acaactgaat
    15181 aaactttatc aaacaacgac tgaagttctt atggaaagac acgggcagat ccagcaagaa
    15241 ttacaagcac aaattccaaa atggaaaaaa gagtatgaaa ttcaacaaca agaattgaaa
    15301 gaacaagaag aaaaacaacg acaagaagaa aaagagcgcg aacaattaga actaaaaaaa
    15361 cgtgagcagc atcttgcaga acaaaaagtg gcgatagaaa aacaacggtt agagcaagaa
    15421 aaggaaaaag aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgacaa
    15481 gcacagttga attatctagc acaacctcaa ccagtagctg ctgttcaaac gccagtgaac
    15541 aaccaagcaa atttaactcc ttcaccacag cgttccttta acggatggat ggtgggatgt
    15601 attgcaagtt taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat
    15661 caatcttcac aggcagattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa
    15721 caagcaagaa aagagtatga acaacaattg gaagaattat tgaatcgtac caatcagtca
    15781 acagcagagt caagtatgga aaatagcaaa gagagtgatc ctagtaaggc taaagaagga
    15841 tcaagcgatt caactgtaca ctcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa
    15901 taatgcgtat tagagaaaca acgagacgaa aagacaaacg attcaattta gaaaagaata
    15961 ggtatctttc tcatttctat cgctatactc cttcaggaga agagctacca gaaaaacatg
    16021 acaaacgtat ttctcaaaag attttttata gtggcttagc ttatcttcct ttaaactatg
    16081 ttgttacaag aataccagaa gcatttcatc gatttgagat tattccagga agtttacaag
    16141 gagtgaatat ttttcagtta caagaaatgc cgcagttatt ccattttgat tcaactggtt
    16201 tttggaatcc tttgacccca acaacaagtt taggcacacg tttagttgtt tctacgattt
    16261 gtgttgttcc agctgtactt atgggattga aaatggacat gtggtttcgt ccgctagcag
    16321 atgggcaaaa aggagataac cgattaaaaa cgttaaagga aatccaacaa atttatccgc
    16381 aaataccaga atcaaaggga agttttcctg gttatggcgg aataccaatt actcattata
    16441 aaaaatactg gtatattcag acggatacag taaatacttg tattgttggt acttcccgct
    16501 cagggaaagg ccagatagaa gttttagcaa ccattgacaa tttgtcgcgt gcagaaagac
    16561 aaagttcaat ggttgttaat gatccaaaaa cagaattata tgttgctagt aaagatgaac
    16621 tagaagctag agggtatgat gtgtatgcct ttaatatcac cgatccactg caatcaatga
    16681 gtgataatcc attggcactc attgtgaaat actggaagcg aggagacata gacaccgcaa
    16741 cacagttaac caatactttt acgaatacga tttattatga tgcaaatgct tcagacaata
    16801 aatactttaa cgaaaatgcg cagaaagctg tcaatgcgct tatttttgcg ttattggaac
    16861 aagcggatca aacaggggat tactccaaat taacaccaaa taatctcgtt gaactacttt
    16921 cagaaattgg cggttttaat tattatccag atcctgagaa tgaattcaag cagctcaatg
    16981 ccttagatga gttcatgaat cagttacccc ctggaaatat agcaaaaaaa caatatgggg
    17041 caacaaaaat tggatctgat aaagcgaaag ggaatatctt gtctacggca attacaggat
    17101 tgaatccatt taccctaacg aaaattgcga agatgacttc tcaatcgacc attgattaca
    17161 agtccattgg ttttccgaag tatttggatc tcaaattaca tgaaagccta ttaaatcaac
    17221 gcattgtcgt ggaattttat cataagggaa agaaatttca cgaggaacga gtcaaagttg
    17281 gctatcgtgg tttttgtgaa atcaactttg attgtcattt agagcaggga gatcatgttt
    17341 gcttaaaata caatcatcaa ggaaaacaat atgttgcttg gtatgagttt tctcaaaaag
    17401 tattgaaaga cgaagaggga aaaatcgttt atcgaaaaaa acgaggagaa actcatttgc
    17461 cagaactaga aaaagaagcc gttctaacat tagatgagaa gaaaagtaac ctattagtag
    17521 agtctattcg gatgcactat accgataaac caatggctat ttttatgtta acgcctgatt
    17581 atgatccatc taaccatatc attgttagca tctttcttag tcagttgtat aaagaattat
    17641 ctacgcaatg tgttaagact aaaggagata aatgtcaccg taggattcat tttattttag
    17701 atgaatttgg aaatatgcca ccgatggaca acatggaagg aatcatgacg gttacggcag
    17761 gtcgtaatat gttatgggat ctgtttattc aatcttatca gcaactttat gcgaagtatg
    17821 gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac acatatttat attatgtcga
    17881 cagatgataa taccatcgaa gaattttcta aaaaagtggg gaataagacg gttgaacaag
    17941 agaacagtac catcaattca ttaggtatga atactcatat caatcgcaat gttgattcag
    18001 atcgtatctt aactcctgaa agaatttcta cttttttaga aggtgaaacg attgtactcg
    18061 ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg gccgtatcct atttttaatc
    18121 atggggaaac gaatcttcct tatgcttatc aatatattga agatttcaat ccatcgtggg
    18181 atatcaatga tatagacata cattgtacac atgcggactt ggatcttaag gctttacaaa
    18241 ttgattatga ttatttccta ccagatgaat atgctagaaa tctctatcat ggactgcatg
    18301 atcaattaga agaaaaaaac gaagaaaata tgttttataa aatcgttcaa tcttttgaga
    18361 atgagacggc taaaaaattg ttaattcaat tttacgaaga acaagcaacg gatcgtcttc
    18421 aagaatatgt tgaaaagttg atcaaaaatg aagaaatttc tgaaatgatt aggaaacggc
    18481 tgttattgga agctgaaaag gcggtgaaag aatgatcatt ggaggaattt tctctggcat
    18541 agccgactgg tggtcaggtt tattcggaga aggggatgca gatcccaaaa ccgtaacgaa
    18601 agtcttacaa gattatggta attatctgca gtatcaagat gtattcagtt ggatttgtaa
    18661 tactttttta tggggattga tcaaagggtt atataatctc aatactttat tagaaaaaac
    18721 aatttatcaa agctttgatt taaaagactt attgaacgcc gctggtgtta atgaactatt
    18781 tcaaagtttg attagtaagg tattggctct tttttgtgtc gtcacattaa tttatttagg
    18841 cttgaaattt tttatgagta aacacccacc gaaagtcaaa aatatcttgg ttaatttatt
    18901 tatggccatg atcttgattt ttggtggtag ttctttaatc gatgaagggt taaatatcag
    18961 taaaaacttt tatggagacg tcactgcagc aaataaagca gataaaacgg gttcacctgc
    19021 ttttcaaatt attcaaaata atgtgattga tgtcagtaca ttactagcga ctgacccgaa
    19081 taaagtagaa tccttaccaa cagataaaag aaatgcttta acagcgaaaa atttcaaaac
    19141 agcgaatatc aatgggatta ttacacctga tcaagctaag aaaatgtctg aatcaagtga
    19201 tattagtaca gtacaaaaag atcggttaaa ggcattacag tatcgactag agttagacga
    19261 taatgggaaa gaaatccctg ttcaaattag tgatgaaggt ttagcaaaat atgtgtatac
    19321 gtcaggctat cgtcgataca ttagtagtcc agggattatt ttggctgggg aatcttcttt
    19381 gtcggtagcc tatttattta ttttgtttac aatcgttact tgcattattg agctagtatt
    19441 taaaaaattc tatttagtaa ttgccgcgtc aacggacttt gagacaggac aacggatgaa
    19501 aagtgcgata gaagatattt ctcaatcctt cttgttattg gcgtttacga ttttagaatt
    19561 acgaatctat acattaatgc tttcaggtat cggtgatttg catgcggtag gaaagatcaa
    19621 tggattcttg tatgttgttg ctttaattgt gctaactatt gcgctattca aaggctctca
    19681 atcggttacg aagattttcg gtgttgatac gagtctgaaa aacggcagca attctcttct
    19741 tagcatgttt gccttaggaa atattgcgaa aaatgtgggt agcggagcta aaaaccttgc
    19801 aagtgctggt aagagtggtt taagccattt aaatgatatt cgtaagaatg gatttggcca
    19861 tcgaaaagat attacccatg ataacgggga agaacaatca gacgataaag ctacaactgt
    19921 acctaatagt aagaagacag tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa
    19981 agccgcagaa ggtcttggtt acgtcaatga acgtgggtta aaaggaacag ttgaagatct
    20041 tacagaaaaa gccaaagaca ccttagaaga gaaattagac ggcacgagag ctaaagaatt
    20101 tgctgaagct gtgaagaatc catccgaagc actagaaaaa caaaaagaaa aagcttcagc
    20161 agtcaaagaa ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaggc
    20221 aaatacaaca agtgaaaaag aaaatcaaga aggtgcagcg attccttcag ccaaagatca
    20281 ccaagatgag ttacattcta acgaaaattc gatcggagaa aaagattcag aagaaggaca
    20341 acatggtcag ttaccaaaaa ccgatccaga acaatatcgt tataaatcta acattaattc
    20401 tgaaaacgaa aaacgcattt cagatttacc aaaacgaaat caagaagtac aaacggatga
    20461 ggattccatc gatctgccaa atgataatgg catcgaagtt ggaactgaaa aatcaacgaa
    20521 aattggtttg cctaagacac aaaatttacc aatgaaagat agtcgtttga atcaattcaa
    20581 agagaatgat caaagctttg ttcatcaatc acctgcaggt actgttcaaa tgaaatcaag
    20641 tcgtttgcct gaaaatggaa atctgacaac tgtttcttac aatttagctg aaagtggtga
    20701 aacagagaat gcgtccgttc aaaatattgt gacaaaagaa gctgcaccta agccaatcaa
    20761 taaagttcat aatggataca gaccagcaaa cgtagcattt actgcaccga caaaaataaa
    20821 tgtgacaaga gaaactaaaa acaggaacaa tgaattgaaa gaacaactat tgaaggatat
    20881 caaaccataa aaggcctatt taagggcctt tttctttata aaacaagttt aaaatggaaa
    20941 ggagaaaatt gtggaggata ttttaatagc tacgtttata actgcactgg gagcattttt
    21001 aacgatattt gtggtgacta ttaaaggatt taaagatacg gatagtttac aaaaagatca
    21061 tcagtctatt ttagatggtc aaaaaacaca taccgaaagc attattgtac acctttcaga
    21121 aggattcact tatttgaaag atgctatgcc cacattcaca gtaaaattaa cggaaatatt
    21181 tcattctgtt gagcaaatat cgtcagaatt gaaagagcaa agacaacaaa aagatcgtca
    21241 acttggctca cttaatttta ggcaagaaac gatacaagaa tcattggaga atgtaaatcg
    21301 attaaacgat gaaatgcaac gagttcattt ggaaaatgaa gaattacgcg cacaaaatta
    21361 tgatttaatt caaaagaaaa gaaatctgga acaaaaaatt gttgaattaa aagaagaaaa
    21421 tcgtcaattg aattatcaat tacgacgagg aaaaaatcgt tctcgtgatt taggaaatga
    21481 attagagttg taatatttag aaaaacgctg tgagaaatca cagtgttttt tatttctaaa
    21541 aatacaaaat ataaggggta tggttatatt atggataaat acattcatat gaaaaatcct
    21601 ccaagattta gtggggaatg gaagtttttc gcctttttct ttttagatct attaacgctt
    21661 ggtggctgct ttttttgggc gaaaatagtc aataatcttg tccacttacc actctttttt
    21721 gcactcttga attatggatt atggcttgcg atagcgttag tgattgtttg gcgaccctat
    21781 aataaccctg gtcaacgcca aatttttgcg attatcgaat ctattttctt tacggataag
    21841 aaccactatc atgcgttaga tcctaaccga aattatcatg gtacgtataa ggaggatgaa
    21901 taaatgcgag tcaaaggaca aaattcagtt gataagaaat ccattttgca gtttggaaaa
    21961 ccagaagagc gctctatctt ggacttattt ccatataaat cctgtacaga agagggagtc
    22021 ttaattacta gagaagatca ttttcagcgt tattatcgtg tcgtttccac tgacgtggag
    22081 ggattaaatg aacaagagaa actagaacga atgaatcagt tgaccaccgt catgcgtacg
    22141 tatgtgccaa caattaagtt agttagtttg accacagaga cggatctaac ggaacaaatc
    22201 gtgcaaaagc ggcagttatt agataaaaat cgtatggaac agtcactagg aagaaatcta
    22261 cagcgattaa gaaaatatga aaagaaatta gtagaggaga tccaggaatt aaaacgggca
    22321 gaacaagaac gtcctgactt aagcttcttt ttcgttattg aaggaaagac aatcaaggat
    22381 ttacaaacta gaaatcgtca gttaatgcga tctagtggca ttttagggtt aaaaccactc
    22441 tacaaaaaag aattgattaa gattctttat cgaatgaaca acatgaatga cgaataaaaa
    22501 agatggagga aatgattcat gctagatgtg ataaaaacag ctttttcaac gcaaagtaat
    22561 caaaaggtga ttgatccatt aaaacaactt ggtaatcgtg aaatagagat tttaaaatca
    22621 aaaggttatg atttagattt cttaaaacaa gtgatgccac aaggtgggtt gaattttgat
    22681 gaagaatatg tgacctatgg aaatggttat tcacgatgtg tgacagttta tcattacaca
    22741 aaaaatccta ctcttttttg gttggccaat ctcatgaaca atcctcatac agtggccaca
    22801 ctagatgtta gcacagatga aaagcaaaaa gtgattgatc aaatcaaccg cgcattagat
    22861 gaattaaatg atcgatcaga taatgaacga aaagcgactg accgaaataa aagtgcggaa
    22921 gaatacctgg aactagcgga atatgcccga aaattgactt ctaatgggga aatttctaaa
    22981 caagtcaaag tgagagtaca tgtttacgga aatacgatcc aagaagtgga tgaacgggcg
    23041 caggaactga tcgaaaatct aaaagggatg gattatcgtg cggtgactta tctgcataaa
    23101 accaaacgag attggcaatc gatgtttacc atgttcgatg aacaagctga atggtttgga
    23161 aatatcaaag ggcaatcact tccatcgtta aacattggcg gaggattccc gttccaccac
    23221 caaacgctaa aagatccagg aggcacacaa tatggggaaa ccatgacagg cggcccattt
    23281 gtctggaatg ccacgtataa aaatagtgta cgtttatcta ccaatacctt gcttctagga
    23341 atgatgggag ctggtaaatc gaccgttctt aaaatgatcg cggaagccca tttagctgca
    23401 ggagacttta tttggggctt tgaaaaggga aaagacttta ttcctttctt aaaggaatat
    23461 aacggtatta tggtgcgttt agacggatct gacgggatga tcaatccttt agagatcttt
    23521 gccacacgga cgtatgatga agcatccagt ttatatgacg atggttcagt caaagatttg
    23581 aagatcaatg aagcagcgtc ctatcaaacg cacttagaca aagtagtcta tcaggtacag
    23641 ttagtgagtc ctcagctaaa aggaacgatg aaagcagaat tcaagaccta cttgaatcgt
    23701 ttttatgaag agtatggaac agtaccacgc ggttttactt cctcaaattc gcgttcaaat
    23761 actgatacgc aagtcacagg gaaagatcca gaagcttatc cgacgttcaa agaattttta
    23821 gcctttttag gacaactgga actaccaggg gcttcccaag agaaaaagaa tcggaaagaa
    23881 gaaatggagt ctatcgtgga atccttatgt gagacttatg gcatgatttt tgatggtcat
    23941 agtacgattc gacaccttga ccaacaacag ttggtctttt ttgacattga ctctattaca
    24001 ggcctagatc gaggggtaca acaatgtcag atgtacatgg ccatgacgtt gatttggaat
    24061 caggcattga ttcaaggacg aagacaacga cgcttaatta aagaaaaaca aatggaagaa
    24121 caagacttca aacgcttctt ggcgatgatt gatgagtgcc ataatgcgat taatccagat
    24181 ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag aaatgcgtaa agtagaagca
    24241 atgactgttt tagctacaca atccccacaa gaaatggtgc cagaaaatat gtcttctgat
    24301 aacttctcga aactgaagaa agtctttgaa ttttcttcta cgaagatctt tatgcgtatg
    24361 gatgaatcta ttttacgtca tatagaagga ttagttggta aatcaatgac gaactcagaa
    24421 ttagaatcca ttcctcaaca aaatcaagga gacgctgtga tcaattttgg ctcaaaagaa
    24481 accattcgtg ttcattttag tccgaacaaa cgtcaattaa aattgtttga tggaggaaaa
    24541 taatggaaaa ccaaaatgaa agtttaatca aacagtatgt taagcgtcgt gcaaagcggc
    24601 gcttattttt gtgggtgttt ggtacttctg caggtttgat tactatattg atcactgtgt
    24661 ttgtgaccct ttttctaata cttgcggcag gttctattga taattctgat tcaagtagtg
    24721 gtggcgaagc ttttacagga gaatattcgg aaggcttacc catttataaa gaaatcaaag
    24781 gaagaggccc tttttctgat gagattgcgc aatatgctgt aggtgcagca gtgaagtata
    24841 aattattacc aagtgtgatc ctttcgcaat acggttatga atcagcgttt ggtacgtcgg
    24901 cgtccgcaag aaatgatctc aattattttg ggattacgtg gtttgatggg tgtctgtttc
    24961 ctaaaggaac tgcgcgtgga attggtggca ttgaaggtgg ctggtacatg aagtttccca
    25021 actcaaaagc tgccttctcc tattacggct ttatggtagc cacacaatca aattttaatg
    25081 cttgtgtcgg aaataaaagc cctggcgcaa gcttattgat cttaggacga ggagggtatg
    25141 ccgccgcagg aattactgaa gacagtgcct attataaaaa ttgtatgtct attattaata
    25201 caaataaatt gatggagtac gatgagtttg cgattaaaca ctggagagaa ggcggcggaa
    25261 ctggtggtac gattacagga agctggggca atccattccc aggaagtagc ttagataaaa
    25321 attcgttcag tggaggacaa ctttttggga aaaatccagg aggagaattc cgacctaacg
    25381 ggttccatga tggactagac tttggttcag tcgatcatcc aggaagtgag attcatgcgg
    25441 ttcatggcgg aaaagtcgtg tacgttggca atcctggtat ttcaggtttg ggtgcgtgtg
    25501 tgatcgtgat taattacgat ggcttgaata tggtgtatca agaatttgca aatagtacag
    25561 gaaattctcg agtgaaagta ggcgatcaag tgaaagttgg tcaagtaatt gggatacgag
    25621 atacagcaca cttgcattta ggatttactc gaatggattg gcgtcaagca caaggacatg
    25681 cattcattga tgatggtaca tggatcgatc cgttaccatt tttgaatagc agcaaaaaat
    25741 agatcaggag aaaaaatatg gaaaataaaa ccttaaaaat cgtcttaaca gctgcaatta
    25801 gcgttagttt aatcggcaat attgcgttgt tttggcagct taaagaagaa caagagacaa
    25861 atacctataa aattgaagaa gtggctaaaa acgcgtctga taagctcaaa aaaaattctt
    25921 ctgaagcaca ggcgaagatt gacaagttaa atcaaaccat caatgagaaa aaccaagaaa
    25981 tcgatcagct gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa
    26041 gaaagacggc agaagagttc ggtcaattgg ccattgataa atcattaagc agaaatgaat
    26101 taacggaaaa actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgtag
    26161 acgatgatca ccaacatgat gattacggaa gttactcttt tacattaggt gacgtgcaaa
    26221 cttacgctca aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt gttgattata
    26281 caatcggcaa tcctcaattc aaagatgtca aaccacaaaa aataaaaggt ggattaactg
    26341 tcactgaaaa acaagtagat ggtcaatgga aagtcacaga ctttagttat ttcactcgtt
    26401 aggaggaaga aaatgagtaa gaaaaaagga gcaagacaac gttttaaaaa acgaaaaagt
    26461 tatcttagat ggctggggat ctttatgatt ctcggctttt tgattcttgc tagtccgatt
    26521 tacacacata aaaaggtaga cataccagat gaagtaagca cgaacgcatt tttaaacacg
    26581 aaggaactga cgatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt
    26641 gcgatcgacg atgaagatca gagcttatta aaagaactag ctaatcttaa ttatcgtgta
    26701 gaagtaaaga cagctaaagg ggattatcaa gcaataaaag ccaaagtgat caaggtgtct
    26761 gatgattatt ttgtagtgaa gttagcacat gtaccagaag aatatattgc tatgcgatta
    26821 actattattc ctgaaaaaat tgatccaaag gtggatatgc aagaaccgca agatctgatt
    26881 ttttatattc acgaagataa ggtcaaagat cacgtgaaag acgaagatta tgaacaacat
    26941 gcgatcaatt acaaagtgaa aggatacaag aaagaacaaa aagcagcaca aaaacaaatt
    27001 gaatccttcc aagcaaccat tgatttgaat gaagaattag tgaagaaact aaaagatcaa
    27061 ttgccttatc aagtcgcaga agatcaggaa aatactgaaa ataagattaa tagttaccag
    27121 caagaaattg aaacatcgaa aacgcaaatg aaagatcaag aagagagcat tcaaaaactt
    27181 caagaaaaaa ttgatcgtat gacaaaagaa aatatttaga agaataggtg gagtaaaaga
    27241 aatgaagcga gcgaacttgg tcggcaaaga tactttcgct gagcagaaag tattgattaa
    27301 agcctatctc aaagttgtaa aagctaaaga aatatgtcaa gaagtgaatt atctaacaca
    27361 acaatatctg agttgaagaa tggtagaaag agtttagata ccttaaaatt cagatcattt
    27421 gaagctctat atatagctgc taaaaaatat tttacagctg ataataaata aaaaattaag
    27481 gtgggttcta tgtgaaaaaa acgtatcaaa agtttttatg gattagctat atcgtcctat
    27541 ttatcgttgg gtttctcgtc agtgtgtccg tgatcgtcgg aacgcttttt cttttgttct
    27601 ctgacgaaga tctggtactt aaggggttaa tgagtacgtt atcgtgtgtc atgttacttt
    27661 atttatgtat acagatcaac gaaattatgc cccctcattg gcaagaaaag aagaaagaat
    27721 ttaaggaaga aatcgaaata aaatccaatg actggattga atacacatgg cttcctctga
    27781 aaagaaatag caaacgaaag gaattttggg cattgcaatt atggccaata ggaaaacttt
    27841 ttatttatat ggcttcactt atggttactt taatcggtta tttggtggtg gtctatgggg
    27901 tggcctatta cttgttaagt gagccaaaac agatcgcggc gtcaaatatt ttcacggcag
    27961 taatgacatt aatgtgtgtc atgtgctttt ctatttttag ttacttactc gtccaattta
    28021 tcaggcaaaa tttaacaatg agtaaacttt tcaaaagttt attggactta cttatcttag
    28081 gcagtatgtt aacgactcta acagatcggt tcgaacaatc tgaaaaaacg gtagagggga
    28141 taaagaactt cttaacgacg attgatcaat ttataaatgg atatatgtat ttgtttgaag
    28201 taggctttat tctcactttg tttggttgtg tatttataaa actcattaaa agatcaatcg
    28261 aaaaagaaat ggttattgat ctaattccga cgctgagcgg ctatgagtat tacgttgatt
    28321 ttggaaagta tgaggagttg atccatacgg atatcgttca cacgagtatc catgaagtaa
    28381 ccgttaaggg catcagatct actttaaaag atggacagga gacgaaaaca atcgaccgaa
    28441 gaaaagaggt ctacaatttt tataaagttg aagcaagtct acgatatggc attgggaaat
    28501 tgaggttaga tgggagtaat attcaacaaa acaaaccata tgttttagga aaagtggtga
    28561 tttagaactt cctatgaaag atgaataaat aaggagaatc ttttgaaaat gattcttctt
    28621 tacagaattg tgcttatagt tggcacggag ttaaaaatct taaagaagga gcagttattc
    28681 aaagtgaaag aacatcataa tcagttacta tgtattaaaa cgggtacgat gaacattcaa
    28741 taccgaacag aatatttaga attaacgaat caaactgatt ttttgtcgat tcgagagcaa
    28801 cataagcaag aaaaattaat ggctacttat agccaattga ttgatgagtg ggaacaattc
    28861 aaggaatcta aaacataaaa ttccatttaa acaccttatt ttttaaagag cataaggaaa
    28921 cgtgtaccca ctttcaaaat aaagtgggta gataggctgt gcctatcacg tttctgaaaa
    28981 aaataaatga ccccaaaatt ttcaatttat gtgtaggttt atgggtagca aaaatatgaa
    29041 tgtgggcagc aaaaagttca tttgtgggta gtaaaattca aatgtgtgta gcaatgtgtg
    29101 tagctacaca cattgctaca cacgctataa catatagtga aaaggagata tttatggaag
    29161 taaaaataag agatattgat gaaaaaacga ttcaaagaat tgaccgaaga tgtattgaat
    29221 taagtgaacg caccgggact aaatggagcc gaaatcgata cctaaaagtt ttgattgaaa
    29281 atgattttga ccatgaactt atggatcata gaaaagatca atttgatcgg ttgttagaaa
    29341 aatttgtgga tattcaaact tataatacga aaattttaga agaatatatt gcaacaaata
    29401 atcaattaat cgccttgtta attgaataat aaaaatagta ttgtgaattt ataaaaggag
    29461 gaaatcaatt ttggcaaaag caacaagaaa acatgtatat ctttacgata tgcatatcga
    29521 agtacttgat gaaataattt ctactcatga agatatatct aattatagtg aagcagtcag
    29581 acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa ataaatccaa ttcaaattaa
    29641 attgaatgca atgggaaaag aaatttctat attaaccgaa atggtttcag agtttgcgga
    29701 tcttcatttg aaagatacag gaatactaat tggtacaaat tcaactgtct atcaagaagc
    29761 aaaagagcgt gtggaaggga aaataaaagc gaatcaaacg aaaaagtatt ctcctaagat
    29821 caagaaacca ataaatgaat ctgttcatgc cagagtagac ccgtttgatc caacaaagtt
    29881 tttaaaaaga taaaaagtag aaggggaaca tgatgaataa atcgcctgca gtcattctca
    29941 cttgtcaatt taccttgccc aataaaaaat ctttttcaac atacattgat tatatgacaa
    30001 gagaaaaagc gctggaagaa aaggaacgac gcacgccgga cgaagaacag gaactgcaac
    30061 gaatcaagaa tgctttagag gatttttata ttcctgaagg agaaactttt tcggagagta
    30121 agacagataa agaattcagt gaaacgtcga atgaagctca atcgttgatg gaacatggaa
    30181 tcaattttga caagttagag gaacaagatt ttaccaaata tctttcttat atgacccgtc
    30241 actatgcgtt agaacaaaag aaagaactga cgcaaaggga acaaaaagaa taccaacggt
    30301 tgaaccaagc aatgaaaaaa tatcaaacag agccacataa aaaagcttca ggaaatcttc
    30361 ctggagtttt ttctatggct tcagacgaag tgaaaggaag tgatctgaag gagatcaaac
    30421 aaatcttcca aagagggcaa caaaaaggct caatcattta tcaagatgtt gtctctcacg
    30481 acaattcgta tcttgaaaaa atgggcttgt acgatcccaa aacagatacc ttagatgaag
    30541 aaggattaaa agcagctgga agaaaaatga tggagacatt attcgagaaa gaacaattga
    30601 atgaaacagg ttactggatg gcaacgattc accgtaacac taagcatatc catattcatt
    30661 ttgcagttgt tgaaaaagaa aatacgcgta aaccttacac ggaggttgaa aatggtgtga
    30721 gttatatcgt accgaaaggt aagagaagac aagcaacatt agacgcaatg aagaccaatt
    30781 atgtacatga gttagaacgt ttttcttatg aaaaggatcg aatgattggt atggagaaac
    30841 gaaatttatt gacgcggaaa agtgatttgc gtaacacctt aacaaaagtg gtaaaagagc
    30901 cagcgcgtta cgatcgacac gcaatgaact tattaaatga agtatataaa accctacctg
    30961 acaaacgttc ggaatggcat tatggggatg aaaaacgaac caaactttca ccatacacaa
    31021 gagagaaact cgatgagtta accaaatatg tgttaacaaa agaacctgaa tatcaagaat
    31081 atatacagat ttcaaaatca ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga
    31141 gagaatcaaa ggatgcagaa aaaaatgctc tctttgattt gaagaagcgc acaggaaatg
    31201 ccattctaac agaactgaag aaacaggata gtcaaataaa acctttaata gaaagtatgg
    31261 atcagatgca atctatttac aatgaacgat cctttcgaac gcttaaacag ttaccaacga
    31321 atgaatacaa atatagttac gaagaattca tgaagcgcag gaaacaattc caacaaagag
    31381 ttgtcacgca acggagtttg cgtcaattaa atcgaacgat taatgcccat gaagaaaaat
    31441 accacgcaag taaagcctac gaagagcaac aacgaagaat tcaaatggaa caagaaagac
    31501 agtaaagaca gtaggaggaa tagcaatgaa acaatattta gatcaatgga aagttataga
    31561 aggatcatta agagaagaac gaatcgaaca acttcccgat tgtttagaaa aagagcattt
    31621 gtttcagata agagaaatgt tgagaaacga acagtttgat ccaaatcaat ttttagtcgt
    31681 tgagtactca gctactggtg tgtactgttg taatcatgtg aagggtgaaa aatattttat
    31741 cattcaagaa tatgagggca agttagcgcc ttattatact acttgggaaa tgaatgaaga
    31801 ggggatcaat aacttcccgt gtaaaagcat tgaagagtct atttcactca cagaatgtta
    31861 gttgttaagt gaataaaaga agtaaaagag tcctgaacgt aattgtccag gactcttttt
    31921 ttcaatgaat tgttttccgc caattcaatg agtggtgcga tttgcgacta cattatatca
    31981 aaagaaagag aagggaggaa gaagaaatgg cttattcaac gattggagtt gttgttgaaa
    32041 aaagcagaga caatctcgtt tttgtgacag aaattcaaac gggcagagct tttgtcgtca
    32101 cagacaaagc ggcaaaagcg tatcaaagtg gcgatatttt aacgttaaat ctgactacga
    32161 agacgttcgt cgatgcggca gaagattatc catt
//
LOCUS       DACCDF010000029        30587 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCDF010000029 DACCDF010000000
VERSION     DACCDF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.029"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..226
                     /locus_tag="IVK51_001299"
     CDS             <1..226
                     /locus_tag="IVK51_001299"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP6430904.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IVK51_001300"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IVK51_001300"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IVK51_001301"
     CDS             533..1336
                     /locus_tag="IVK51_001301"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP6430905.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(1585..2649)
                     /locus_tag="IVK51_001302"
     CDS             complement(1585..2649)
                     /locus_tag="IVK51_001302"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP6430906.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(2646..3731)
                     /locus_tag="IVK51_001303"
     CDS             complement(2646..3731)
                     /locus_tag="IVK51_001303"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP6430907.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IVK51_001304"
     CDS             complement(3744..4721)
                     /gene="mvaD"
                     /locus_tag="IVK51_001304"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP6430908.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IVK51_001305"
     CDS             complement(4714..5658)
                     /gene="mvk"
                     /locus_tag="IVK51_001305"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP6430909.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5980..6633
                     /locus_tag="IVK51_001306"
     CDS             5980..6633
                     /locus_tag="IVK51_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP6430910.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            6718..7623
                     /locus_tag="IVK51_001307"
     CDS             6718..7623
                     /locus_tag="IVK51_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6430911.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(7625..8257)
                     /locus_tag="IVK51_001308"
     CDS             complement(7625..8257)
                     /locus_tag="IVK51_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP6430912.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            8577..9101
                     /gene="infC"
                     /locus_tag="IVK51_001309"
     CDS             8577..9101
                     /gene="infC"
                     /locus_tag="IVK51_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP6430913.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            9173..9373
                     /gene="rpmI"
                     /locus_tag="IVK51_001310"
     CDS             9173..9373
                     /gene="rpmI"
                     /locus_tag="IVK51_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP6430914.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            9426..9785
                     /gene="rplT"
                     /locus_tag="IVK51_001311"
     CDS             9426..9785
                     /gene="rplT"
                     /locus_tag="IVK51_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP6430915.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            10137..11375
                     /locus_tag="IVK51_001312"
     CDS             10137..11375
                     /locus_tag="IVK51_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP6430916.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            11423..13552
                     /locus_tag="IVK51_001313"
     CDS             11423..13552
                     /locus_tag="IVK51_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP6430917.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            13527..14216
                     /locus_tag="IVK51_001314"
     CDS             13527..14216
                     /locus_tag="IVK51_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430918.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEIYEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(14624..15310)
                     /locus_tag="IVK51_001315"
     CDS             complement(14624..15310)
                     /locus_tag="IVK51_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6430919.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(15446..15640)
                     /locus_tag="IVK51_001316"
     CDS             complement(15446..15640)
                     /locus_tag="IVK51_001316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430920.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(15690..16130)
                     /locus_tag="IVK51_001317"
     CDS             complement(15690..16130)
                     /locus_tag="IVK51_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP6430921.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(16134..16979)
                     /locus_tag="IVK51_001318"
     CDS             complement(16134..16979)
                     /locus_tag="IVK51_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP6430922.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            17128..18546
                     /gene="pepV"
                     /locus_tag="IVK51_001319"
     CDS             17128..18546
                     /gene="pepV"
                     /locus_tag="IVK51_001319"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP6430923.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(18622..19824)
                     /locus_tag="IVK51_001320"
     CDS             complement(18622..19824)
                     /locus_tag="IVK51_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430924.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(19811..21598)
                     /locus_tag="IVK51_001321"
     CDS             complement(19811..21598)
                     /locus_tag="IVK51_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430925.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(21611..22564)
                     /locus_tag="IVK51_001322"
     CDS             complement(21611..22564)
                     /locus_tag="IVK51_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6430926.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(22749..23099)
                     /locus_tag="IVK51_001323"
     CDS             complement(22749..23099)
                     /locus_tag="IVK51_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6430927.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            23299..24378
                     /gene="pepA"
                     /locus_tag="IVK51_001324"
     CDS             23299..24378
                     /gene="pepA"
                     /locus_tag="IVK51_001324"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP6430928.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            24521..24841
                     /locus_tag="IVK51_001325"
     CDS             24521..24841
                     /locus_tag="IVK51_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6430929.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            24863..25327
                     /locus_tag="IVK51_001326"
     CDS             24863..25327
                     /locus_tag="IVK51_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6430930.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            25523..26128
                     /locus_tag="IVK51_001327"
     CDS             25523..26128
                     /locus_tag="IVK51_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP6430931.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(26167..27456)
                     /locus_tag="IVK51_001328"
     CDS             complement(26167..27456)
                     /locus_tag="IVK51_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP6430932.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            27884..28363
                     /gene="rlmH"
                     /locus_tag="IVK51_001329"
     CDS             27884..28363
                     /gene="rlmH"
                     /locus_tag="IVK51_001329"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP6430933.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            28716..29030
                     /locus_tag="IVK51_001330"
     CDS             28716..29030
                     /locus_tag="IVK51_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6430934.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            29046..29432
                     /locus_tag="IVK51_001331"
     CDS             29046..29432
                     /locus_tag="IVK51_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP6430935.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            29461..>30587
                     /locus_tag="IVK51_001332"
     CDS             29461..>30587
                     /locus_tag="IVK51_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP6430936.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRD"
BASE COUNT         9687 a         5721 c         6239 g         8940 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggacagaagc gtttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
     1501 tttgtgaatt tcagcttatt tccgaaggag ttgcttttgc ttccgccgtt tatacgtgtt
     1561 tagagcgtgg gataaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
     1621 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
     1681 cagtgattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
     1741 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
     1801 atgcgtcagt acggttcctg aagtacccac tgcatttgcg ccgagacaaa gggctttaaa
     1861 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
     1921 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
     1981 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
     2041 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
     2101 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtctctt gaataagtac
     2161 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
     2221 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tcttgggctc tttcgacaga
     2281 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
     2341 ataagtatcg gctaaagaag ggtctttcaa ggcagcgctg actgaaccag tggcaatcat
     2401 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
     2461 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
     2521 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
     2581 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
     2641 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
     2701 tctgcttttt cccatgcact cattaaagga aggatgccag atttctggtc aacaattacg
     2761 attccgcaat cgccaccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
     2821 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
     2881 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
     2941 ttaaaccctt tgatcatttc gttgacacat tctgtactgt tttttaagaa aagctgataa
     3001 gccaccattt tgtcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
     3061 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
     3121 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
     3181 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
     3241 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
     3301 atctccaatt gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
     3361 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
     3421 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
     3481 aaacgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
     3541 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
     3601 acttttcgtg cggattctac agtgactgtt acgaattgat cgactgcagc gataactgct
     3661 ggatgtcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
     3721 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgggca
     3781 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
     3841 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
     3901 cctttttttc tcaattgccg caccgcatcc atcgctttta agctgtcagg agaccagtaa
     3961 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
     4021 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
     4081 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt tcgacgcatc
     4141 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
     4201 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
     4261 ttttcccatt caacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
     4321 gcaaatcttg aaagagagag atcgtctaat ccaagcttaa gagcagtatt gcaagctcct
     4381 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
     4441 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
     4501 tgactgactt tttttgttgc tttttcgtct tgtaaggtgc catctagata aaattcatct
     4561 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
     4621 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
     4681 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
     4741 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
     4801 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
     4861 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
     4921 tgtcagcttt tccagaagtg actgtgattc atccattgtc tgtgccaacg tttctggtga
     4981 attctcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
     5041 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
     5101 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
     5161 gtcaaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
     5221 gattccacta ggattaccgt gggcgatttt ttccgaaagc tggacatttt ctaatagtat
     5281 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
     5341 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
     5401 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
     5461 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
     5521 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
     5581 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
     5641 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
     5701 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
     5761 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
     5821 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
     5881 aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaaggtt ggagttaaac
     5941 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
     6001 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
     6061 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
     6121 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
     6181 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
     6241 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
     6301 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
     6361 catattcgtg tatcttttga tacgatgtta gagcgtatcg aaaaacgtgg tcgttcgtat
     6421 gaacaattgt cctttgaccc aagtctatac gattattaca aagaattgaa cagacgttat
     6481 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
     6541 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
     6601 gcagagattc gccaaaccaa agtatccatt tgatttacag ttgctcaaat aaagggtaaa
     6661 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
     6721 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
     6781 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
     6841 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
     6901 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
     6961 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
     7021 attcgactaa ttcgatcgct tttggaacgg atgccagaca tgagtgtata cagtttattg
     7081 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
     7141 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
     7201 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
     7261 cttttagaac aaatcaaggc actggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
     7321 gagttggacg atgcactgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
     7381 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
     7441 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
     7501 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg ggaaatcgcc
     7561 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
     7621 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttgat
     7681 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgttgtgaag aaattagtcg
     7741 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggttaac tgattgtcag
     7801 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
     7861 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
     7921 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
     7981 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
     8041 caaaaatgac attactgcat tcaaaatgac gtaaacctgt tttttgcatc gtaagttttt
     8101 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg ttacctccgt
     8161 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
     8221 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
     8281 agtatagcaa agaagcggga atcactgcaa gaaagcctgc taatctaatc attttcagaa
     8341 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactataat
     8401 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
     8461 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
     8521 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
     8581 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
     8641 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
     8701 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
     8761 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
     8821 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
     8881 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
     8941 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
     9001 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
     9061 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
     9121 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
     9181 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
     9241 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
     9301 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
     9361 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
     9421 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
     9481 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
     9541 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
     9601 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
     9661 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
     9721 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
     9781 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
     9841 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaataac
     9901 ataattaagg ttttttaatt gaaaactgaa catttatggt catcctttaa tgaaagcgct
     9961 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    10021 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    10081 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttt gtcatcatgg
    10141 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac tcggcgatgg
    10201 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
    10261 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
    10321 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
    10381 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
    10441 gaccagtgat ttctattgca gttattgtgt tgccgatctt gatgtcatta ggtatttcag
    10501 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
    10561 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
    10621 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
    10681 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
    10741 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
    10801 gggtcgttct tttagatctt caaatcatct taggttttat tctagcaggt atctgggcat
    10861 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
    10921 tatttacaga tggcgcagtc gatgttgcac caatggttgg tttcttgatg accttagcga
    10981 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
    11041 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
    11101 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtgttagctg
    11161 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaattaca acgattgctc
    11221 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
    11281 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcggt gcgatcttat
    11341 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
    11401 tagataaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
    11461 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
    11521 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
    11581 aaattgtttg cgggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
    11641 cacacaagcg actaatgcac tgattgaagg agatgtagca aaagtcggcg taatcgggat
    11701 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
    11761 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aaaacttaaa
    11821 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
    11881 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
    11941 atctgtgaaa gcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
    12001 aagaagaact cggacagcag caatcaacgc atctatttta ccaaaaatgc tagatacggc
    12061 aacctcgaca gaagactccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
    12121 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
    12181 ttccggtcct gctgcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
    12241 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
    12301 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
    12361 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
    12421 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
    12481 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
    12541 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgttct
    12601 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
    12661 acaggcatta gcagactatt gtcatacgac ggttgaagac atcgcagagc agatcatgga
    12721 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
    12781 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
    12841 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
    12901 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
    12961 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
    13021 accagaatcg atcgaagtcc acgtagaaat cgatccacat acttcaaaag taacggctat
    13081 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
    13141 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
    13201 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
    13261 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
    13321 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
    13381 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
    13441 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
    13501 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
    13561 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
    13621 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
    13681 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
    13741 gagaaatcta tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
    13801 aacgaatcac ttttgcaact tatgcagaag atgaagggat ccgattgatg acggagccgt
    13861 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
    13921 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
    13981 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
    14041 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
    14101 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
    14161 aagaaataaa agaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
    14221 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
    14281 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
    14341 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaaaggtg ttcaaaagct
    14401 tgcatctgcg gtaataagaa cagccaagtt aaaaacataa gaagctattt ttacttggct
    14461 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
    14521 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
    14581 tatttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
    14641 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
    14701 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
    14761 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
    14821 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
    14881 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
    14941 cttttttatt ttgagcgatc actgcctgat cggtttgttc tagtattttt tctagtttag
    15001 cttgatcttg cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
    15061 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    15121 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    15181 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    15241 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    15301 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    15361 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    15421 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
    15481 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    15541 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    15601 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    15661 atacaagaaa aatgcttaga cggtatttat taagaattta catcatccaa agaagcgtac
    15721 gcttggaata actgttctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    15781 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    15841 cccacaattt cgtagctaaa atccaaccgt gttcccgtat acttttggat agttgggtgg
    15901 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    15961 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    16021 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    16081 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    16141 tcgttttcca ttgatttttt acaaaaataa ttcatccact tcggaagtgc ttcgctgatt
    16201 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    16261 taaacagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    16321 atcccagcta aagtgtcacc catgccgcct gttgccatag cagcagaacc taaaggattg
    16381 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    16441 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    16501 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    16561 aaaagatgat tagcaacaag taaacttaaa gcagagccat caatcaccag ccattgttct
    16621 tttgattgtt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    16681 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    16741 gtccaatcta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    16801 tcagttacta cagtcgtgag acccgctcca ctgtgtacgg cagctaaagc actcataatg
    16861 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    16921 tgtgaaatct ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    16981 gaaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    17041 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    17101 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    17161 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    17221 gattcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    17281 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    17341 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    17401 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    17461 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    17521 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    17581 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    17641 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    17701 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    17761 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    17821 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    17881 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    17941 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    18001 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    18061 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    18121 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    18181 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    18241 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    18301 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    18361 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    18421 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    18481 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    18541 aaataaaaat actaaaaaac tccggctatc tctgacagct ggagttttta tgtttaaaaa
    18601 tgaattagta catcggtatt cttaaaagta atttagttcg tcttttgatt tctcaggagg
    18661 gatcaaaaag gcgaaagcac taagtatagc aaaacttgtg atgatttgcg tacgatacgt
    18721 aaaccaataa tgaatctgtg agtgattagc taaaatattg taccaaatat acggcgttac
    18781 agcagctaaa acaaacaaaa ctagaagttg gaacggacgc cagctatttc gtttacgata
    18841 agcaaaatag agaaaaacaa gacaagtaat caatagcaat aatattgcta cttttggata
    18901 catcaatcta atattgcttt tcagcatctc taatcgatgc aacgggtatt tgtcgtttcc
    18961 ttccgttcga aagaggattt ggttgattgc atcttttaca atgtttttgt tcaatatgat
    19021 cgatgatagg atccatttag ccacccatgt caaaccatag cctaatcccc aaaagatacc
    19081 ggtaaaaaca aaactgccta cttgtttgct caatgaactg cgtttatcca ttaaatactt
    19141 atttgttaca taataaataa tgatcacagg tatcccccaa gagatgaccg gtgttgtcaa
    19201 caagtcaaaa aagttagcta aagaaccaat gacaaagaaa tacaagaata attgttggtt
    19261 ggtgttggag agttgtggtt ttgttaaaaa tagtaataca gacaataaag tcaaaatcca
    19321 aatatttgaa aattgcatac tgaagaaaaa ggttgcagca tttcctatag caagcgaaat
    19381 caacaaggct gtcgttaaat agatgtcgct acacttggtt aaaaagtagc tcgtcaatcc
    19441 aatcagcaga aaaagaatgg ctgcgtaaat cattcgaata ctccccaaac tcatccctag
    19501 cagtaaaggt cgtaaaaaaa gctgataacc atgccaataa cggggataat ccgccataga
    19561 catcgcgttc tccaaggagt tcaattcagg atctgctttt gtttttcgta tcatcaaatg
    19621 atcagtaaaa ttatctaacc gagtgcctag catatttcct tgattgatag aaggatacat
    19681 tccttctttt tcaaccatct gtagagattt ttctgcattc gattcaatag agtgctgcgg
    19741 aatgaaaaaa gtaattgtca gtagtacgca atagctagcg atcaagagaa caaaagcaaa
    19801 aaatatcttt ttaaatagtt tcatgagtat ctcctatatc tgttttcatt ttttctcttt
    19861 tatctagtaa aaaaggagag aggatataaa caggtagacc taatgctaaa acaaacacat
    19921 accgtaaact aaaagccaca ggagtagcaa tcataatagt agcaaaaaca agcataccgg
    19981 gaagaaaaag gagtaaatac cgatattttt tcttaaagat tgctaaaagt gcactgccga
    20041 tagctaacca cagtaatgtg ccggaaccaa agaaatcacg agcatcgatg atttttttca
    20101 ttcctgacaa tccagtaaga tgttcaatca aatctacttg cttcatacca taattattcg
    20161 tgtttacacg agtatctaaa aagccgtaac tattttttgt accaggtacc cagaacccga
    20221 acgtttctaa aatataagca tctgtataga tttttatatt acttggtaaa tttcttgccc
    20281 atagtgatag aaattctttt ttatgtgtat tcaaaaactc attgttaaaa tttttatgcc
    20341 acttgattga gtctgcaaga ctaggagaat acgcttcata atcttttatc gggagtagct
    20401 gattcatgaa tgctttatct ttcttagaca agtcaccatc cataacaata gttctagaaa
    20461 tttgctgcaa aggaacacct aattgttcag cagtttctgt aggaatagca taacgagcca
    20521 tcaacgggtg gatgagtagt ccagcaatca ctgtataacc cagcaccact tgaaagaatg
    20581 cttgatacct tttttgtttc aaaataatca agccgcaaat caaacctgtg ccggttacta
    20641 cataaatccc attatttctt agataggtgg tcagtaaagc aaggatgatt acgtaaatct
    20701 gattgagaat cgttggcttt tctcgttgaa aaataaagtc aaaaaccgct aaggaaaata
    20761 aaaagcaagc gatagagaac aaagtatctt tttgcatgtt caaggcgtag attggaaaaa
    20821 ccggtgcaaa tgcaaaatag cataaactaa tgagtaccgg aaaaaaatgg atgtttcttt
    20881 ttaacagcca gcaaagagaa taacttatcg ctgaagctgt tatcaatgtt tgaaaaatcg
    20941 aaaataaaaa tacaccgtga ttgatcgtat cagttggtaa atcatgaaca aatagcttga
    21001 ccagtaaggt gaataagact ggatgatgat tgcttatagg aacatcaccc atagattggg
    21061 cgaccgaact taacgaatca ggcaaaacca ctccaggata caatgccaac agataaggaa
    21121 gccaacacag aactaaaaag acagtaaaga ataaaaaata ttttcttgaa gagaaagcct
    21181 gtgtgttttg agcagtaaaa aatgtttcca gttttggcat tgataaataa atcgattgta
    21241 aaaataaaaa agaatacaca aaaatggaga aataaacaag aaagtaaaaa atacttccat
    21301 cagcaatcgt tgtctttgta tagtttgctg agacatactg aaattgtatc tgtctgccaa
    21361 tagaaagtgt aagagcaaaa gctgcagaaa aaaacaatcg ggcaatagct gcaactttgt
    21421 tttttattga aaacaactct attccatatt gcttaaaagc aagtagcaaa accatggcta
    21481 aaccaatgat tactgttgga ttcaaagttc ctagaccata agaagtaaaa aaagcaaaac
    21541 ctgacgcaaa taaagttgca aaaaaagtag gaaatagatt ctcctttttc ttatgcataa
    21601 aaaaggcctc ctacttcatt tctttatttt gatattcgtg gtagacatgg tataaattca
    21661 attcatattc tttcttggca tttgtgacga ccgtatccaa aatcaaccct gttactaatg
    21721 aaagaagggc taaagtcatc aatccagtac ttaaaatcgc agaagggatg cgtgtaataa
    21781 agcttgtcag catatattca cgaatcacag gaatccccat gaccaatcca aataaaaaga
    21841 agaagatggt ccagatccca aagaaaagca aaggtttata atctttgcac attttgatga
    21901 tcatcatgat cactttgaat ccgtcagaga atgtattcaa ttttgattcg ctaccttcag
    21961 gacgatcgcg ataatcgatt ggaatctcaa cgaacttgaa ttttttatct aaagcatgaa
    22021 tcgtcaattc tgtttcgatt tgaaaaccag aactcataat agggaagctt ttgacaaaaa
    22081 ttcgattgaa tcctcgatag ccggtcatga catcgcgaat ttttgtttta tataagtaag
    22141 taattgtatt tttaaccaga ttattcccaa aatcatggaa aggacgctta ttttcatcaa
    22201 aataggttcc atttgataga cgatcgccaa tgaccatatc tgcttcgcca gaacgtaatt
    22261 tctcaagcaa accatgtgca gcttctgcag gataagtatc atcaccatct accatcaaat
    22321 aataatcggc atcaatatca aaaaacattt gacggattac attccctttt ccttgacgtg
    22381 gttctttctt tacgattgct ccgccttgaa cggccaattc gtatgttcga tcggtcgaat
    22441 tattatcata gacataaata tctgcttctg gtaattccct tttaaaatct tcaataacag
    22501 ttgaaattgt cgcctcttcg ttataacaag gaatgagcac ggcaattttt tcacgtgatt
    22561 ccatttgtca ctctccttat ataataaaac taatcttatt tcaataatac cgtataataa
    22621 ctacaaatac tagctattcc aagaggaaat agccctattt ttataaaaat cacccactat
    22681 aaaagaagga taggatacct ctcttttaga tagtgggtgc gtattcgtta tttcattttt
    22741 ttgacgtatt aatctattgc gccctttttt cttgaaatgt ctaatacggt tcctgtatga
    22801 gcatctgcta aaaattcata ataaacagtt tcgccatctt ctatccggga aagcccaccc
    22861 cgataagcac tcgaccgaac ggcgaatttc cgaataggtt ctttttcaaa agagatccaa
    22921 gcaccttcga ttgcgccttc ttttaaaaaa gatgttttga tattttcaag cacttggtct
    22981 gctgataaca cttttttgcg atgataccaa atcgttgaag ctaccccact caataatcct
    23041 agaccgatcc caacggctaa cccgccttta aaatagctta attcgctttc ttcatacatg
    23101 tggatatacc tccttcttct cacttgctac tctttgtgtt tattttacca cactctcata
    23161 ttttctttga aataaataac cgctaccaga aaaaataagc tataataaat acgatcggtc
    23221 tgcagaaaaa atcatatttt atccgcagat caatataagt ctgagaggag tttagtctga
    23281 aatttagacg aatagcatat ggaagaaaaa acatttcaac gaatcaaaga gttaacagaa
    23341 ttacaaggaa caagcggttt tgaacaagac gttcgcgcct atatggaaag tcatatggaa
    23401 ccgctggtag atgagttaca gttagatggt ttgggcggca tttttgggct gcgccaccat
    23461 gaagaagcgg atgcacctcg tgtaatggtc gcagcacata tggatgaagt cggattcatg
    23521 ctgacacaaa tccaagaaaa cgggcttttt aaagtcgtac ctctaggcgg atggaaccct
    23581 tacgttgttt ctgctcaacg ttttacactt aaaacctcta ctggtaagaa ttatccatgt
    23641 atttcttctt ccgttccccc acatttatta cgaggaacaa gcggacaaaa atcagtagaa
    23701 gtaacagata ttttgttcga tgctgggttt gaatcaagag aagaggcaat gagttacggt
    23761 gttcttccag gagacacaat cgttccttac gctgaaacga tcaaaacagc caacgggaag
    23821 aatatcatca gcaaatcgtg ggacaaccgc tatggctgta caatggtatt agaagcttta
    23881 gaagcgttac aaaatgaaac attaggtcat acattgattg caggcgcgaa tgtccaagaa
    23941 gaagtaggct tgcgtggttc taaagcttct gtcaataaat tcaagccaga tctgttcttt
    24001 gcagtagact gctctgcagc agatgacaca gtgacgaaaa atggcacatt cggacattta
    24061 ggagaaggta cattgatgcg tatccaagat ccaggtttga tcatgcttcc tcgtttgcgt
    24121 gagtacttgc tagatatcgc tgaaacaaac aacatccctt atcaatactt tgtttcaaaa
    24181 ggcggtacag atgcaggtgc tgctcataca caaaatgaag gaatcccaag tacagtgatt
    24241 ggcgtagtgg gacgttatat ccatacgcat caaacgatgt tcagtattcg cgattttgaa
    24301 gctgcacgag aaatgttgat tcaaacattg aaaggtttag ataaatcaac agtgaacacg
    24361 atcgtatacg ggaaataacg cgatagtgaa actataaaat ctggcaaaag caaccttttg
    24421 tcagattttt tttataaaaa gaggatcaat gtttgttagt tcgaaagaaa ggtgtataat
    24481 ttaattgaaa aatacataaa cagaaagtag aggaaaaaga atgattatcc caaatagttt
    24541 agaagaatta gcaggctacg tagaaactgg aaaaagtgta tttttcttta ctgccggctg
    24601 gtgcggagac tgccgtttta tcaaaccgca aatgcctgaa atcgaaaatg acttttctga
    24661 ttggcaattt attgaagtag accgtgacaa gtacatagat gtagccgctg aatggaacat
    24721 ttttggtatc ccaagttttg tcgtgatcca agacggaaaa gaattaggtc gtctagtgaa
    24781 taaagatcgt aaaacaaaac aggaaatcga atcgttccta gaaagtgtaa taaaaggctg
    24841 acatctgtta ggaggggtca agatgaatca agaatataag aacattttag taggtattga
    24901 cggtagtgaa caagcacttg ctgctttcaa aaaagcgtta gaagtggctc gccgtaataa
    24961 tgggaaagta tatgtagcaa acgtaatcga tcagcaattt tataatttta tgggctatgc
    25021 tcctattgat caaaatctag tcgatcagca aactgaaaca gccaagcaaa tgatcgaaga
    25081 atgtaaagaa tatggaaaat ctgttgatta cacaaatata gaaggaatta ttgcatacgg
    25141 ctctgttcgt gaatccatgg cagtcaaatt gccaaaaaaa tatgcaatcg atctaatcat
    25201 ggttgggcag tctggactaa atgcagtcga acgctttatg acaggaagtg tagccagcta
    25261 cgtcatacgt cattcacctt gtgatgtctt gatcgtttct gacgaagaag aacagccaag
    25321 taattaagca agtggaactt gtataacctt tttaatcaaa cagtaaaggg actgtgacaa
    25381 aagtcttgtc acagtccctt gttacgaata aatggtgttc cagaagaagc ttcttcggaa
    25441 ataaagcatg aaaaaaatct catgcagtga atgcaatttt ttgttaagat aaatctcagc
    25501 aaaggataat ggaggaaaga acatgatttt tgcttataac aaagcacacg tcggagacac
    25561 tttgatggtt atcatagccg acgataaagg aacagaaaac actgtcgaac gtaaatggaa
    25621 tgttgctcgg atcaaagatg aacaaggtca agttgttgct tggaactttt tccatatctc
    25681 cgatcatcta acaattgaag ggaacggcca agtaagctta acagatgaag aattagcgaa
    25741 attgaatcga ttgattaaag aagcaggatt cgaagaacag cttgaagctg atcatgaacc
    25801 gaagatcgtt gtcgggtatg taaaaacatg cgtcccgcat cctgactctg accatttatc
    25861 gattactgag acagaagtgg acaatgggac agtattgcaa attgtttgcg gtgcaccaaa
    25921 tatcgaagct ggacaaaaag ttgtcgttgc aaaaccagga gccatgatgc cagatggaat
    25981 gatgatctgg ccaggaactt tgcgaggggt agccagctat ggaatgattt gttctgctcg
    26041 tgaattgcat ttgccaaatg ctccagaaaa aaaaggaatc ttagtattgc ctgaggatgc
    26101 cgtagtcgga gaagtatttc caaaataaag aggttgtgac aagtctttgt cacaacctct
    26161 ttattattaa ttttcactat tttgattttg ttttaatgcc gattgatcga tcgtcaattc
    26221 aacagtagtt gttttttctt tcgagccttc atagtaagtg attttgacag aatctcccac
    26281 ttttttctta tacaaagcag attgaagttc tacaccagaa gaaacttctt tgtcatcgat
    26341 cttagtaatg acgtcgtatt gtttcaaccc agctttttct gccggtgtag ctgtttggac
    26401 atttgtcacg atgacaccgt tagttacaga tgaaggaatc ttcaagattt gttcttgttg
    26461 ctgagtagaa acggcagata agtcgaccat tgtgatacct agagctggac gtgtcacttt
    26521 tccgtctttt tccagttggt tgatgatatt cactacatcg ttacttggga tggcaaatcc
    26581 cattccttct acgcttacat tagaagtcga ttcagaagta cttgcgatct tacttgagtt
    26641 gataccgata acttgtcctt cgatattcac taaaggacca ccagaatttc ccgggttgat
    26701 cgctgcatct gtttggatcg cattgatatt gactgtttcg ttcgattcat tcgtacttgt
    26761 tacttgtcgg ttcaaggaag aaatgattcc tgaagtgaca gaattcgcat attcagatcc
    26821 taatggggaa ccaatcgcaa tcgcaggttc accaaccttc aaagcacttg agtccccaaa
    26881 agaggcaacc gtttcgactt tgtctgaatt gatcttcaac accgctaaat ctgaataggc
    26941 atctgttcca actaattcag cttttacctt cgtaccgtct ttcatcatga cttccaaacc
    27001 ttgctgtcca tctactacgt gattgtttgt gactacataa gcagtattcc cgtctttttt
    27061 gtagattacg ccgcttcctt cactataagc ttccaaatta ctatcatctg agctgctact
    27121 ttcttcttgc tgcccgaata atccgccaaa accgcttgat tggttttgac tttgtaagtt
    27181 gataacagaa acgacagcgt cttgaacttt atctaccgct ttggtgatat cagaatctac
    27241 attgactttg acattttcca caactgtttc accggcagaa ttttgattgc cgcttgtagc
    27301 agtagaacta ttgttgccag tgcccatcgc tgcataaaaa atgccagctg tcagtagtcc
    27361 tccaacgatc cctccaacaa gccccagacc caacttacgc cagaggctat tatttttatt
    27421 ttttttcatc tttggtgtca cattttttct atccatgtgt tcctcctcca attacattca
    27481 tttatctact gttagtatag tcttatttgt tgactttagt ctactttgag actttgaact
    27541 ttttatgaaa aactttagga aaaatcattg ttttcagatg aaaatatttg attttctttt
    27601 tccacagata ttgtgtaaaa ctacgcggat aggattggaa aaataaggga taaaacatat
    27661 ccacagcctg tgtataaagt ggataaattc ttataaaacg tatgtttgcc taatgaatgt
    27721 gatgaaataa ggtaaaacaa cgctgttttc tgtggataaa cctgtggatg ttgtgcataa
    27781 ctgttgggga aatcttttta acaaaaatag tcaaaataaa catatagacg aaaaaattga
    27841 ttataataaa caaagtgagc gtgaagatca aaggagagaa aacatgaata tcaaaattat
    27901 ttcagtagga aaattaaaag aaaaatactt gatccaaggt atcaatgagt atgtcaaacg
    27961 gctgaatgcc tatgcgaaaa tcgaattgat cgaagttcct gacgaaaaag ccccggagaa
    28021 tttaagcgaa gcgcaaatga gacaagtaaa agaaaaagaa ggagaacgga tcttagcgaa
    28081 aatcaaagaa caagaatacg tctatggctt agcaattgaa gggaaaaatc ccacaagcga
    28141 atcttttgct aaacaaatcg accaattagg tatccaagga aaaagccacc ttgtttttgt
    28201 tatcggcggt tctttaggac tcagcgaagc cgtcatgaaa agaagcaatg ctcagatctc
    28261 ttttggcaaa atgacgtatc cacatcaatt gatgcgcctg atcctcgtcg agcaaatcta
    28321 ccgtgccttc cggatcaacg cgggggctcc ataccacaaa taatgatatt ttttaagaga
    28381 aaaatagcat aaaaatctag ttatccgcat aaaaactgga cttatcacac tttatcaagg
    28441 tcaaaaccac tcaatttact actaatttac tacttatgaa tgagctttga tacgacgatt
    28501 tatccttgaa aagtgaagat ataaagatac ttccaataaa atttgaatat ttaataggta
    28561 gacacttcaa aaaatgaggt gtctattttt ttacccgatt ttgaaaggaa gtgaacttat
    28621 gaaaacaaaa aatcaagaat caaaaggtcg ttccccactc tttaagacca tcaaacattc
    28681 attcagccaa taaaaaagaa aggataggta aaaatatgga acttaaattt gtgattccca
    28741 acatggaaaa aacattcggc aatttagaat ttgctggcga ggataaagtc gttcagcgaa
    28801 gaatcaacgg acggctaact gtcttatcaa gaagctataa tctctattct gatgttcaaa
    28861 gagcagatga tattgtggtg gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg
    28921 aggaacgtgt gaagttagtc aatccacgta ttaccgcaga gggctacaaa atcggcactc
    28981 gtggttttac aaattacctt ttacatgctg acgacatgat aaaagaataa agaaagagag
    29041 gaaaaatgat gagattagca aatggcattg tattagataa agacacgact tttggagaat
    29101 tgaaattctc tgctctacgt cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag
    29161 atgaaatcaa ggaacgtacc tatgacttaa aatccaaagg acaaggacgc atgattcaag
    29221 taagtattcc tgccagcgtg cctttgaaag agtttgatta taacgcacgg gtggaactta
    29281 tcaatcccat tgcggacacc gttgctactg ccacctatca aggagcagat gttgactggt
    29341 atatcaaggc agacgatatt gtgctgacaa aggattctag ttcattcaaa gctcaaccac
    29401 aagcaaagaa agaaccgaca caagacaaat agtcgctagg tagaaaggag actttttcgc
    29461 atgaaacagc gtggtaaaag gattcgccca tctggtaaag atttagtctt tcattttacg
    29521 atagcgtcac tcctgcctgt tttcctgctg gttgtcggac tgtttcatgt gaagacaatc
    29581 cagcagatca actggcagga ttttaaccta tcacaagcag ataagattga cattccctat
    29641 ttaattatca gtttcagtgt cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa
    29701 cgggttcgct atgatacggt taaacaactt taccaccgtc aaaaactggc aaagatgata
    29761 cttgaaaaca agtggtatga atctgaacag gtcaaaacag agggtttctt taaagatagt
    29821 gctggtcgta caaaggaaaa gataacctac ttccctaaaa tgtattatcg acttaaaaat
    29881 ggcttgatac agatacgggt ggaaatcacg ctgggaaaat atcaagacca actcttacac
    29941 ttggaaaaga aattagagag tggcttgtac tgtgagctga cggataaaga gttaaaggat
    30001 tcctatgtgg aatatacttt gctctatgac accatagcca gtcgtatttc tattgatgaa
    30061 gtagaagcta aagatggtaa acttcgctta atgaaaaacg tatggtggga atatgataag
    30121 ctccctcata tgttgattgc tggtggtaca ggtggcggta aaacttactt tatactgaca
    30181 ctgattgaag ccttgcttca tacagattca aaactgtata ttcttgaccc gaaaaatgct
    30241 gaccttgcgg acttaggttc tgtgatggca aatgtctact atagaaaaga agacttgctt
    30301 tcttgcattg aaacattcta tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg
    30361 aagaactata agactggcaa aaattatgct tacttaggtc tcccggcaca cttcttaatc
    30421 tttgatgaat acgtcgcttt catggaaatg ctgggaacaa aagaaaacac cgcagttatg
    30481 aataagctga aacagattgt catgttaggt cgtcaagctg gcttctttct aatactggct
    30541 tgtcaacgtc cagacgcaaa atatttaggc gacggaatcc gtgatca
//
LOCUS       DACCDF010000030        29740 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCDF010000030 DACCDF010000000
VERSION     DACCDF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.030"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(192..662)
                     /locus_tag="IVK51_001333"
     CDS             complement(192..662)
                     /locus_tag="IVK51_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP6430937.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IVK51_001334"
     CDS             992..2632
                     /locus_tag="IVK51_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP6430938.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IVK51_001335"
     CDS             2786..4108
                     /locus_tag="IVK51_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6430939.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4607)
                     /locus_tag="IVK51_001336"
     CDS             complement(4176..4607)
                     /locus_tag="IVK51_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6430940.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEA
                     KKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4672..5508)
                     /locus_tag="IVK51_001337"
     CDS             complement(4672..5508)
                     /locus_tag="IVK51_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP6430941.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6077..6382)
                     /locus_tag="IVK51_001338"
     CDS             complement(6077..6382)
                     /locus_tag="IVK51_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP6430942.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6407..6838)
                     /gene="ruvX"
                     /locus_tag="IVK51_001339"
     CDS             complement(6407..6838)
                     /gene="ruvX"
                     /locus_tag="IVK51_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP6430943.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6835..7101)
                     /locus_tag="IVK51_001340"
     CDS             complement(6835..7101)
                     /locus_tag="IVK51_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP6430944.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7356..8081)
                     /locus_tag="IVK51_001341"
     CDS             complement(7356..8081)
                     /locus_tag="IVK51_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP6430945.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8442..8512)
                     /locus_tag="IVK51_001342"
     tRNA            complement(8442..8512)
                     /locus_tag="IVK51_001342"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8478..8480),aa:Gly,seq:ccc)
     gene            8629..9381
                     /locus_tag="IVK51_001343"
     CDS             8629..9381
                     /locus_tag="IVK51_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP6430946.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9397..9876
                     /locus_tag="IVK51_001344"
     CDS             9397..9876
                     /locus_tag="IVK51_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP6430947.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10127..11707)
                     /locus_tag="IVK51_001345"
     CDS             complement(10127..11707)
                     /locus_tag="IVK51_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP6430948.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(11700..12443)
                     /locus_tag="IVK51_001346"
     CDS             complement(11700..12443)
                     /locus_tag="IVK51_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6430949.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(12619..13395)
                     /locus_tag="IVK51_001347"
     CDS             complement(12619..13395)
                     /locus_tag="IVK51_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6430950.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(13643..14479)
                     /locus_tag="IVK51_001348"
     CDS             complement(13643..14479)
                     /locus_tag="IVK51_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP6430951.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(14490..15419)
                     /locus_tag="IVK51_001349"
     CDS             complement(14490..15419)
                     /locus_tag="IVK51_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP6430952.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15611..15964
                     /locus_tag="IVK51_001350"
     CDS             15611..15964
                     /locus_tag="IVK51_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP6430953.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16038..16694)
                     /locus_tag="IVK51_001351"
     CDS             complement(16038..16694)
                     /locus_tag="IVK51_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6430954.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            16920..18854
                     /locus_tag="IVK51_001352"
     CDS             16920..18854
                     /locus_tag="IVK51_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6430955.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(18940..20163)
                     /locus_tag="IVK51_001353"
     CDS             complement(18940..20163)
                     /locus_tag="IVK51_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP6430956.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20157..22055)
                     /locus_tag="IVK51_001354"
     CDS             complement(20157..22055)
                     /locus_tag="IVK51_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP6430957.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22049..23167)
                     /locus_tag="IVK51_001355"
     CDS             complement(22049..23167)
                     /locus_tag="IVK51_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6430958.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23250..24083)
                     /locus_tag="IVK51_001356"
     CDS             complement(23250..24083)
                     /locus_tag="IVK51_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6430959.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24076..24993)
                     /locus_tag="IVK51_001357"
     CDS             complement(24076..24993)
                     /locus_tag="IVK51_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6430960.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25220..26344)
                     /locus_tag="IVK51_001358"
     CDS             complement(25220..26344)
                     /locus_tag="IVK51_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6430961.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            26527..27435
                     /locus_tag="IVK51_001359"
     CDS             26527..27435
                     /locus_tag="IVK51_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6430962.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            27576..29189
                     /locus_tag="IVK51_001360"
     CDS             27576..29189
                     /locus_tag="IVK51_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP6430963.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9192 a         5825 c         5077 g         9646 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggctttttgg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc tacgccagtc
     4321 atcaaagcct ttccgatgcc tcttttttca gcatatttag acacagcaag tgccataata
     4381 ttgaacattg gatcgctaaa aatcgattca taggactccg cttgtacata gccagctgct
     4441 tttttagaag taggatcttc ataaaccaag aaaaaatttt tctctggctc cttcagcagt
     4501 ttcttcattt gtctcaccgt aacggttaat ggtatatcat accctaattg atccgcattg
     4561 atttcacgta gtgcttctgc atcttctaaa cgcatttccc gtaacatcca aatcaccagc
     4621 caatttctga ttttttaaaa tgaatttttt tatcatagta tactcttttt ttcatttaga
     4681 acaatatgtt tcagtgattt tgacttgcgt tagtctagat tctcctaatg tttgtaagat
     4741 gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg acaattactt gaaaaataaa
     4801 ggtcactttt tttggagaca aaaccctgct aaatcttgct ccccacaaac ctccaataac
     4861 agcagcgggg ataacataaa acaacataga taaatcaaag cgcataaagc tacttgttaa
     4921 agcaatcgtt accaattttg ccagttgaga aaagaaaatc gtagatagag aatatagtgt
     4981 tgcttctttt attggcaatg caaacatcag catcaacaaa gaaacattga ctggtccacc
     5041 accaatacct aaaaaactag ctaaaaagcc cagaaccccc ccacatataa ggtaccaagt
     5101 ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg tagaagaacg caaaaaccaa
     5161 ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc ttctcgtttt caaataactg
     5221 taaaaaaact tcgaatacaa tgttgccgac tactcctcca acaacagctc cactagatac
     5281 ccaaccgaca atctgccaat tcagtgattt cccgcttgat acttgtcgca cagtcgaaac
     5341 aatggacatc gtgaataccg caactgttga gtaaaaagaa atccctgcaa cagaatgggc
     5401 gccgataaga tcaagaatcg gtttgatcaa cacgcctcca cccatcccgg aaatagcgcc
     5461 aactgtgttc gccagaacga tcaccacaaa ataaatcaga ccaatcatct tgtatttcct
     5521 ttctcttgtt tatatctttt agcgattgtt actcttttct tttattatac agtaaaagaa
     5581 acaaacgagg cttcgaaagc ttttctactt tttaaggaaa tttagtgcta aacgaaaaga
     5641 ggttgtgaaa aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagctcctc
     5701 ataattttcg tttggttgaa cttattaccg cagatgcaag cttttgaaca ccgtttaaga
     5761 aagaggtcgt gagcctgccg ttcagctccg agtcacaagg aacaatttca aaaaacagct
     5821 tctcatgttt gttgaatatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
     5881 aggaagagac tgtgaagtca acgttttgac ctgattatta gagaaacaaa tggcaaaata
     5941 gttcttcata tttcgccgtt tttcgagcga atgccgaggg gcagctgatt gaacatcgtt
     6001 tatttggaaa aagggattgt gacaagacgt ttgtcacaat cccttttttt atatcttatt
     6061 aattcattct tttgttctat tcttcttctt ccgccatgaa tgtattgaat acttcttcaa
     6121 tcatgtccca ttctgcgtct gtttcgattt gttctaactc gccttctgtt ccatcttcgt
     6181 tttcgatgta tgcataagct tgtaattcta catcttcatc ttctgaaaca ccagcaggat
     6241 ataaaagaac atagttacgt ccaaattctt cttgtccgtc aactgttagt aggatttcat
     6301 ataatgtttc atttccttgg tcatccacca atgtgatgtg ttcgtgtcct tcatggtcgt
     6361 gggaatggtt atgttctgtc ataagattac ctcttttctt ttattattaa aataattgct
     6421 tacctgagcc gtctaaataa ttttgtaaga tcatcacagc tgctaattta tcgatgactt
     6481 tttttcgttt ggctcttgaa gtatttgcct gctctactag catacgttct gcctgaacag
     6541 tggttaatcg ttcatcttga taatcaactg gcaaggagaa tagttctttg atcttttctc
     6601 cgtatgacat ggaagcttct gcccttggtc cgatggaatt gttcatattt ttaggtaatc
     6661 caactacaaa ccggtctact tcatattgtg ctactaattc cttcaaccga tcaaagccaa
     6721 actcttcttc tgcttcattg atccgaatga tctctactcc ttgagctgtc cagccaaagg
     6781 ggtcactgat agcaacacct actgtcttag aaccgacatc caagcccatt actctcataa
     6841 attcactcca tgatttgaga ggtagtcctt gacaattact tccatgatct catcgcgttc
     6901 gtgacggcga atcaaatttc gagcatcacg ataacgagga atgtaggccg ggtctccaga
     6961 aagcaagtag ccgacaatct gattgattgg attgtagcct ttttcttcca aagcccgata
     7021 aacaatcgct aatgtttcgc tcacttcttt tttgcgacta tcgtcaaaac caaaacgtac
     7081 ggtttcatct gtaaaaccca tgattctaca cctctttccc gcaaggtaat tccagcttac
     7141 cttctacaac cttattttac tcgaagaaag ggaaaactac aaacaaatat tccaaacttc
     7201 cgaataaatt aagaaacact atttgtatgt ttttttcctt ttttctgtag ctttatcaaa
     7261 agtactcttt tcataaagta tacgctatat ttctttgaat gtatgttttt acacaatttt
     7321 ttcataatat tcataaaaaa ttcgaatagc tgctactaat tttttgtttt tgttgcttgt
     7381 cctgtctcat aattgatcgt gtagccatct tgaacattgt agataaagac attgaattca
     7441 atttttttgt cttcaatgct ttctgcttcc aattgcactc ccctggctac cagttcattc
     7501 ccctcaaaat gaggagtgac acggtagcgt acatgatgat ttgtttcttt gatatatgcc
     7561 gcaatatcat tttcgtgagc aagcatgtat ggagcattca acgaacgtgt ccctgtcatc
     7621 aagtttttaa tgttgttatt ttgaccagtc agctggaaac caattaaatg gctgcgatta
     7681 tataaccatt gcccgtcact taatttcttt tgtttccaac ctgtcgggtc aatatacaaa
     7741 ggctctctct tttcagtagg gaacaaatct tttccaagca tagcattggc ttgtcctact
     7801 ctgttcaatt tatcaatatt tgaatagctt tcccatgttc ctttggaaag gctaagatcc
     7861 tcttttgtaa attccggctg attcccattg atttctattt cttgatgttt tccatcatat
     7921 tctggcacat ccgctacttg agaagcttgt ggtgtggaat caaaaatagt tgtaatctgc
     7981 tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga atgcccctgc aatcacagca
     8041 gcgacgatcc ccacgatcgc cttggttgtt tttttgctca tttctttact cctttactaa
     8101 cagctcccta aatcttaaca tatgttcccc tataaataaa tactagaaag aaatagaaac
     8161 agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac aaaaaagaga cggattcctc
     8221 gtctctaatc agtcaccact ctatctgtaa ggtatttatt attattcaaa aattttcttc
     8281 atactcaaga caatacactg atttcacaac cgcttggtaa acggtgcgca aaagcttgca
     8341 tctgcggtaa taagtccatc caagttaaaa acataagaag ttgtttttgc ttggctgttc
     8401 ttattacttg ttgcagagca gctttttcac aacctatttt aagcgggtga cgggaatcga
     8461 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcaatacaaa
     8521 aacagtatac gcctctttta ttccttcctc aagttttttt attctttacg cttctggtat
     8581 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     8641 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     8701 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     8761 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     8821 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     8881 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     8941 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     9001 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     9061 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     9121 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     9181 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     9241 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     9301 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     9361 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     9421 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     9481 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     9541 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     9601 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     9661 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     9721 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     9781 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     9841 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     9901 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     9961 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
    10021 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
    10081 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
    10141 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
    10201 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
    10261 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
    10321 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
    10381 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
    10441 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
    10501 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
    10561 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
    10621 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
    10681 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
    10741 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
    10801 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
    10861 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
    10921 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
    10981 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
    11041 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
    11101 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    11161 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    11221 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    11281 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    11341 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    11401 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    11461 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    11521 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    11581 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    11641 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    11701 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    11761 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    11821 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    11881 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    11941 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    12001 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    12061 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    12121 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    12181 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    12241 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    12301 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    12361 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    12421 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    12481 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    12541 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    12601 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    12661 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    12721 ttcgtttact ctgctaatag atgtaatgtt ttcagtatta atgatataag cttttaaatc
    12781 tttacataaa tactcatatt cgagtgattt tttatatttt ttaattgtgc cgtttacgag
    12841 aatctcttca tctttcaata ctaggcctat tttattttta taacctggca tgctcattag
    12901 ataaaggatt tcatcgtaat ggatatagtg atcgctatta ccataacgga cagttgtcca
    12961 cctttttttc gtagcatgtt gaacaggata gttttctttt tctttcaata agtaactttg
    13021 gatgcttttt ttattttttt cataagtgaa ttcttttatc aaataaccac taggcgtaat
    13081 ctttcgatta ataacagtta ttgctttatt cggatgagac gttagataaa caattttaca
    13141 cttttcattc ttttctctaa gcatttcgcc tagttcgata ccgctataat aggcgttcaa
    13201 atcgatgtct aggaaaaaaa tatctgagga atagatttca ttatttttaa tgaattcaac
    13261 aaattgtata acgttcaatc cctgaacgat ctcaaatttc caaggactta ataaaattct
    13321 ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc aaactatcat ccaaaataaa
    13381 tatacgcgtt accataaaaa gtacctccac tcgatatttt actatcaaaa tcatttcata
    13441 caacttttca acatacatgt taggaataaa ttgtaattct cttcacaatc ttttccaaca
    13501 cctttgaaac cgatatcaaa tactttcagg atgctgcttc taaaataaat atcaaacaaa
    13561 tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct tgcgtgtcag ctgtctgagc
    13621 tatgtttatt tattttgttc ttttaaaaag aacgatacac atactgattt ttcggagcag
    13681 agactttctc agcgtttgta acaactacag atgggatttc acgcttgaat ggttgataat
    13741 agtctgattg gatggttcct tctattttat accattcatc atttttaacc gttactcctt
    13801 ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc cacacaatgg ataatgccga
    13861 atcggaacaa gaagatatct gtattgctat cgttcggtgt ttggtaaaca aatccttcat
    13921 agctgatttt tttgccaatg aattcgctag ggtaattata aattagctcc ataacttcta
    13981 aatagttttc atctgtgatc ttgatagttt gcttatcccc atacttttct ttcaactcat
    14041 tcatttggtc taagtagtct gatttgttga aataaatact ggtatccggc tttaagtatt
    14101 gtgtctgcat ctccggatcc cctactgatt ctttacttac tgggaaatta aatcctttgg
    14161 cttctacgat cgtagtgtcg agactaactg ttggaaacaa cgtccccacg attactggca
    14221 aagctagcag tacataagcg attccacgtt gataaggttt tgacaaccca tggtcatgat
    14281 cgtgctcgtg tgccttttct tctgcttttc cgtttttgac ccacaaatac agctgaacta
    14341 aagccaaaag agcagataaa atcattgaaa gaatagctaa gtagcgataa tggacattga
    14401 tatattgatc gagtttgcca gaaacttgca agtacatcat caattcgaaa tacccagata
    14461 aaatcaaaaa ccgcagcata ttttccctcc taaataaata atgtgaatcc aataacgcta
    14521 agggcaatca gtccgatcag cgataaaata aagcgtcctg taaaatatcg tttcatcatc
    14581 agtagatttt tgatatcgac cattgggcca aagaccaaaa aggcagctac aggtgctgtg
    14641 ccgaacagac ttaacaaaga agatcctata aacgcatctg cttctgagca aagagacatc
    14701 gtaaaggcca ataacagcat gatgataatg gctaaaatct ttgttgagcc gagcgttagc
    14761 ataattctag ttggtaaata agtctgcatc gcagctgcga ttaatccgcc aatgatcaaa
    14821 taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca acacatgccc agtttgatgg
    14881 atcagctgat tttttggttc attcttctca ctttgttctt cttcatggac atgatgatgg
    14941 tgattttctg ctactttaga cgtgattttt tctttcaata cagcgtcttt ttgaaaatag
    15001 gcaagccaga caccaatcac aagggcaaca aaaaagctgc ctatcacccg ccagcttgcc
    15061 atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa tcacgattgg attgatgatc
    15121 ggagcagtca gcataaaagc aaacgctgtg tgaactggta cgcctttttt aacaaattga
    15181 tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga aaaatcctaa gatcgaacca
    15241 gtaaaaatag agaagatacg attttcagga agccatctct tcacacgttc tggggtcaaa
    15301 aatacttgta aggcgcctga gataagacag cctaataata cgaaaggtaa tgcttcaata
    15361 acgatcgata agaaaatcgt actcatctgc aaaaccgaat ttggaataaa agaaaacatg
    15421 agtttcctcc tttttatttc tagttcattc gctttgcaaa tatacgcttt tattcaaaaa
    15481 aaacaagttg ctctgtaaat ttttacacaa ctcttatttt ttaccaaagc acttcaattg
    15541 actttttctg ttattcccat taaattaagg tctcatacat atcacttcta tcataaaagg
    15601 gagaataaaa atgaaacaaa ccactaactt gactgaagtt caagacatac tgcaacagtc
    15661 atccgttgcc gtcatttatc tttcgatgcc taattgcagt gtttgtcacg ctgttcgtcc
    15721 acgactagag gaacttctca cacattatga tattccggca cttcatttgg atgcatttga
    15781 aacaccagaa gtagctagta aatttgaagt gctgactgcg ccagttgtcc tgatttttca
    15841 tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac tttaaaaaaa tccgttatct
    15901 actagatgaa ctgacagcag aagatgattc cctttcttat gaagaaatct tccgaacaaa
    15961 ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg aaagttttgc cttcatttgt
    16021 tcaatcgctt tttattttta ttctgctgta ttttcggaat gcgcatttgc gtcagaatgc
    16081 aagaaataga tcaaggtttg caattcattc gtcaaatcaa cattttggat caatacatct
    16141 gacggagcat tcaatcgagc agcggtaaag ttcaagattc ctttgattcc tgcttccgtt
    16201 aactgattga ctacttcttg tgctctttca gcgggtatcg ttaaaattgc tacttcgatt
    16261 tgttggatct tgatttgttc tgacatatct tccattggat aaacggggat accgtctacg
    16321 atgcgcccaa cgatttcctc attcacatca aaagcacaac tgatacgtat gctattactt
    16381 tgatggaatt tgtattttag taaagcactt cccaagttcc ctacaccgat cagcgcaaca
    16441 tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt gcataagatc ttctacatca
    16501 taaccatacc cacgttttcc taattcgcca aaataagaaa aatctcgacg gatcgttgca
    16561 ctatctactt gaacggcttc acttaattca gttgaagaca cttttttctt tccagcatta
    16621 tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt tagctgtcgc ctttggaata
    16681 atatgatctt tcacgaaatg acctccacgt attgtctaat tacatctttg atttctccat
    16741 catagcagta tttataggaa aaaatcaatt ccattgctca gatattgtga aattttattc
    16801 tttattcatt tatttatcat ttctgcaaat ctcgcagtat aatggaattt atggtagact
    16861 actaattgaa aaatagtttt ttatttgagg agaattcaca aagtagaaaa gaggattcca
    16921 tgatattatt acaagccaat cagattgctc gccattttgg tgcagaagtc ttatttgaaa
    16981 acatacattt agaaatcagt acaggcgctc gtgtcgcttt agttggcaga aatggcgctg
    17041 gaaaatcgac tttattaaag atcattgctg gaattgaagc acctgatgaa ggaaagatcg
    17101 ctaaaaataa aacagcgact ctcggatacc tcgctcagga cacaggactt gattccaatg
    17161 aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt tagacaaatg gaagagcgga
    17221 tgcgcgagct agagattttc attagcgaag gtacgccaga cacacctgct tatagttctt
    17281 atttaaaaga atacgaccgg ctgcaacacg atttttcaga tcgaaacggc tatggttatg
    17341 aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga ttcttcattt tatgatcaga
    17401 aaatcgatac tttatcaggc ggacagcgca cacgtttagc tttagctcgc atgcttttgc
    17461 aacaccctga cattttgatt ctagatgaac caacgaacca tttagatatc gagacacttt
    17521 cctggcttga gaattatctg caaagctata ctggtgcatt attgattgtt tcccatgacc
    17581 gttatttttt agataaagta gtcaatgaag tctatgaatt aagcagaaga aaaatgcgcc
    17641 attacaaagg taactattcg aaatatctag atttgaaagc agaacagtta gcaagtgaat
    17701 ggaaggctta tgaaaagcag caaacagaaa tccataaatt agaagacttc gttgctcgga
    17761 accttgttcg cgcctctacc acaaaacgag cgcaaagccg cagaaagaca ttagaaaaaa
    17821 tggaccggct ggatcgtcct caaggggatg aaaaacaagc aaactttcta ttcagtatag
    17881 aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc cgctatcgga tatgataatg
    17941 tcaccttatc tgaaccaatc aatctagata tccgtcgtca agaagcgatt gctctagtag
    18001 gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat cattcaccag atccctttta
    18061 tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg ctattacgat caaggtcaag
    18121 ctgatctgca tacaaacaag acgatcctag cagaattgtg ggatgagcat cctaccactc
    18181 cagaaaaaga gatccgaagt gctttaggtg ctttcttatt ttctggcgaa gatgtagaaa
    18241 aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt tgctttagcc aagttagcga
    18301 tgaacaaaga aaattttttg attttggatg aaccaacaaa ccatttagat attgacaaca
    18361 aagaagtatt agaaaatgca ttgatcgatt atgacggcac actgcttttt gtttcccatg
    18421 accgatattt catcaatcgg atcgcgacaa aagtcgtcga actctctgaa aatggcagca
    18481 aagtctatct aggagactat gattattatt tagagaaaaa gcaggaagaa gaagaaatcg
    18541 ccgaattgtt ggcacaagat gagccagaat ctgctcctgc tcccacgaac aaatctgatt
    18601 attaccaaag caaagaacag caaaagctta tccgtagcct tcaacgaaaa attactcagg
    18661 ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca gttggaacaa gaaatggtcg
    18721 cgccagaaaa cctggaagat cacgtaaaac tcaatgagct gaaccaagat ttagaagctg
    18781 ctagacaaga tcaagaaaca aaattgacgg aatgggaaga actaagcttg gaattagaag
    18841 aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc atgctgtttt ggtttttctt
    18901 tttttatttt atcaaacaaa aattccatat ttaccttttt caccaataag aagaccaatt
    18961 actagtcatt ctcactaaag cttctaaata atagtaatca ccccaaatac aaggtgtatt
    19021 taccccatta tttttgggct tattataaac agactgtgtt aaaaagccat cttcttcttc
    19081 cgttgtagca tatctttctt tcaattgtcg cataattttc caaacagcag actggtataa
    19141 gttcttttct gttttgtcta atagacctga ttctattaat tctaataatc cacaaaccat
    19201 gatcaccata ctagatgtat ctttttcttc tctgctagaa tcatcaaaga tcaaatccca
    19261 ataacaaatg gtatcttctg gcaaccgatt cataaaataa ttggctaata ttttgcttaa
    19321 atcaaaatat ttttgttctt tcaaataacg ataacttaat gcaaaaccgt aaatccccca
    19381 tgcttggcct ctagcccaac aggaattatc actataacct tgatgcgtac ttccatatct
    19441 tggttttcca tctttgacat ccatataaaa tgtatgataa gttgaaccat cttctcttac
    19501 taaataatct gctgcatttt gaatatgagc ctgagcagct tttttatatt tttgatttcc
    19561 agtttcttca ctcgcccaat acaataatgg aagattcatg caacaatcca tgatcattct
    19621 accttgttgt gaaggatcac taggatctcc ccaagcttgt ataactcctg ccttctccca
    19681 aaaacgctga agtaatgttt ctgcagcatc aattgaagct tccttcattt ttcgatttcc
    19741 cgttatttta taatctgcta cggttgataa agaatacaaa aaaccaagat cgtgatgatt
    19801 agtgataatt tgacagtcaa tcctgtggat aaaatctata tctgcttttt cagctgtttg
    19861 ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa ttaattcctg tccaaaatcc
    19921 ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct tttataggac actcgcttgg
    19981 gaattgatac tgactgttac tgatacgttt gattaacctc tccgtgtgtc tgcgacaacg
    20041 ctcgatctca ttattccatt ctttttccat taattcttta taacattcat ttatccattt
    20101 ttctggatgt attatctctt caaacatcac tttatcaagc atttccccac cagcctttag
    20161 cataatttta gtcttttttt attgttttta ttttggatag ttagcccggt tgtcccatca
    20221 aaatcaattt tgggacgatt gttccatact tctaaactat aatcccgatc ttgatgagca
    20281 accactcctg ttccgagcca atgggttcct tttccaaatg taccctctaa tccaggaata
    20341 aaagtgtttt cccatgtcat caagtttgta ttgggcaaac ccataatagc tgtggatttt
    20401 cgtttaatag tatcgtcact taaacagata atacctgaaa agccagcttc actcacccta
    20461 tagttttcct tgtcttcttc tatgattatt ccttctttct caggagaaca ttcaatagca
    20521 aaccctgtct catatagaac atattctgta tctaattcta ctttgtgtac gcgaatctgc
    20581 cagccttcaa aaggaataag ccaagttctg atttttgcct gtaaagtagg ttcccatgta
    20641 gaggcaagta cctctttctt acatacaatg tctcgtacat tatttcgaac gatgaactgt
    20701 ccataggttt tgatgctctc attcttaaaa ctggtcgtga tatatgtgtc cgctcgctga
    20761 atccctagca ttgaatccat tgccatttct gctttcaatt gattcgctcg cggtatcgaa
    20821 aaaccaaatt gactagaata agcaaatttt aaatattttt cattggtcaa tgtatggaaa
    20881 tttggcccta attgtcccgc attcaaaaaa aatgtatgtc cattatcatg ttgtatgagc
    20941 aaattggctt ctgaaagaag tttagttcct tcacgaatag gcataggcat ttctttagcc
    21001 gtccaatact catggtcttc aggtagcgcc aacaataaaa aaatcttatt cagccataat
    21061 ggagataacg tactattata gggttcagtc aatgtcaatt gagggtaggt ataaccaata
    21121 ttcaggatac ctttttcatc aaatatttct ttttgcatcc accatcttat attccgattg
    21181 actaacccct tgatttcacc taatttaaaa ggcaaaagat cggcccacaa catagctgac
    21241 caaaagcttg aaactgcaaa acgataaatc atgcttcgac caaaaggcac atttgcacca
    21301 tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa tagctcggtt cttatatatc
    21361 tggctaatgg gcttttctgg ttctagtacg ctataaatca aaccataata atgaaatgca
    21421 aaaggattat aataatcttg gcgacctctt gaactgtctc gataccagcc attttgaaga
    21481 taacaatttt ctatattatc aaatgcttct ttcagtgctg attctttaac tggataacca
    21541 aatttttgga taattgttcc gacaattatc ttaaaaaatt gccaattacc atctgcacat
    21601 tcatattcaa aaatttgcga gaaccaatga ataatctggc ttttttctcc tgtatctaat
    21661 tttttccata ataattgttc atgatgatat aacgctaata acattgcggg catttctacc
    21721 atacgctggt ctctttcatg tatttctccc caataatcgg gatgggaagg atctgttcca
    21781 gcttttattc ctgatagaat taatttcaac catcgttcat ctaattggtc aattgcgggc
    21841 cccgctcccc ataaaacccg agagaatcct tccattcctg caattttatt tccatatcct
    21901 accccttgat gtatataatc aatttgtgcg ccacttgctc gataaaacgg ctggagtggt
    21961 tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg tccttaatag tggtactgga
    22021 tgtttcattg tattttctcc tctttttact accaaatagc taattccgac tgttctaatc
    22081 gaagtaagcc ttcaataaaa aaataatcag aatagatcag tggcacatta taagtcagtt
    22141 ctctatggta tgcttctgct ccgcctccaa tgatcccatc ttcatccata ttccaatttg
    22201 catagtgctt ttctgtcttt tggagtatat tgatagcagc ttcatgatac atttttgatc
    22261 gtacagagga aacatatcct gctaattcca gaagaccgca tgcagcgcat actccagcac
    22321 tggtatcaat attcgaatct tttacagcaa aatcaatgag aggaatataa ccagtttttt
    22381 cgattttctg aataaaattt actgctatct tttctgctat agctagatag tacggatttt
    22441 ttgtgtaacg ataacttaac gcgaatccat ataaagccca tgcatttcct ctgctccaac
    22501 aagaattctc attgtatcct tgcccgccaa gtaaccgaat atatttccct gattctgcat
    22561 caaattctgc gatatgcccg gttgatccat ctttcctcac tagatactta gctgttgtat
    22621 ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc agtttccctc gaagcccaat
    22681 acagtaatgg taaattcatc atactatcaa taatcatcca accaggttta tggtcattcc
    22741 atgctactaa atagttccct gaaatattat agcgtccagc aagtaccgta gctgcatgta
    22801 accctagttg tcgactatgt ttgttttgag actgttgata atgtgcaata gccgatggta
    22861 agaataaaaa acctacatca tgatgaacgt ctgaaaaatg gtacagggca gattctaata
    22921 attgttcttg ttgtattgct gcctctttga acttttctcg ttttgtcttg tgaaagagct
    22981 gccataaaat accactccaa aaaccattgg tccaccagtc gattcctctg tcctttatta
    23041 cgtctgtata ttttccgttg taagaaatat atggaatttt attccccatt ctgtctaatt
    23101 ccgcatctat cttttcagaa gctacattca aaatttttct tgaccattcc ttcgtctgtt
    23161 cattcatgga tatcccgcca ttcttttaat ttttatgaat gtggagttgc tcctagaacg
    23221 aactagatgg ttaacattcg ttattttttt tacttatttg cagctccaat gtcattctcc
    23281 ttaaatttag actgcgccca cacataaatc aagaccattg ggatcactga agttgctaaa
    23341 attagcataa ttgcgccata attaattggc tcacctgtac tagaaaccgt catatttttg
    23401 ataattaagg gaaccgtata tttatcttta tctgaaagca tgactaatgg aaatagatat
    23461 tgattccaac ttcccataaa gataagtaaa ccactagtaa taatcgtagg tgataaaatc
    23521 ggtacagcga ttttcgtaaa aatcacccac tcattggcac catcgattct ggcagcctcc
    23581 atcagtgctg taggaaattg actgctatat tgtcgcatca taaacactga agttgtactg
    23641 gcaatcgaag ggatgatcac acctgcataa gtattcgata atcctaaacg actcataatg
    23701 taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc tagatagcat aactgtattg
    23761 aacaacagtt tattccccat aaaacgatac tttgctaatg cataacccgc cattgtagaa
    23821 ctggctgtag ctaaaactgt actgattaca gcaataacaa tcgaattcca aataatcaag
    23881 ggataattat atctcgccat gatggcttgc cagtttttta atagcatatc cccaaacttg
    23941 aaattgattc cgccacttaa aatttcttga ttactttgcg ttgaagctac aatcatatat
    24001 aaatatggca tcaaaaatat taaggcacca aacacaataa tgacgtacgt aaaaattctg
    24061 ctttgtatct tttgtttagt catttttatc acctccgaat ttaaattgaa tagccgataa
    24121 tataacagat acaaacacca tcgtccaggc aatagcagag gcttttccaa aatcaaccat
    24181 ttcaaaactt gttttatata aattgactcc tacgaccaaa gctgaatttt ctggtccatt
    24241 ggaagtccca aagaatatat ttgggatatt gaaattaccc aagccattga tagtggctaa
    24301 aactgtggcc attaaaatga ttggctttag catggggatc gttaataaaa agaattgccg
    24361 aaatttcgat gctccatcta tttcagcact ttcgtatatc tctttagaaa tcccttgaag
    24421 accagctaaa aataaaatag tgttatatcc tacactaagc caaatcatcg tgatgatcat
    24481 tgatattcgt atccaaaaag gatttgttaa ccactgtaca ccgttaattc ctagcgcatt
    24541 caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc atgaatacgg aagctacagc
    24601 caccgttgat gtaatggttg gaatataata gacagttcta aaaaatcctt ttccttttaa
    24661 ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat agcatcgtta ttggaattga
    24721 tcctgccatg ataatcaaaa cattcgtgta agatttaata aacaatggat ctcggaagat
    24781 agctagataa ttgtcaaatc caacaaattc aaatgagtag ccaattttca taaaactaaa
    24841 agcaaacgag ataatgatgg gaatcaaaat cgtcacaata aagaaaataa caaaaggtgt
    24901 gaccaatcca taagcacttt ttttatgaga cgtatctgtt gcttttttct tgctgttctt
    24961 tattgtcttg cttggtaacg aagattgcat catcgttaag tcctcctaat attttttaat
    25021 caccagttaa ttttacgccg tacttatgag caaaattttc tgcttctttt agatatgaat
    25081 tttcaaaatc acctgtttgc caatagttat atgcagcggt agtcaaataa gacgtagcat
    25141 ctgaataagt ggctggtaaa acagtatttg atgtattttc attgatttta ttcaatagtt
    25201 catagtattt ttgtcctcct tagtagtcaa actttttctc agctgcttct gtttgatagg
    25261 ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt ggctaagcat tcttcatctg
    25321 tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt tttggcatct ttagggacat
    25381 aaaaacttga tcctcctgat actgcacctt ttcctggatt ttcttttgaa aaaggaatca
    25441 gtggagccat gcgccatttg tcagatgcat caggaaaatt caaagacata ttcgtagcat
    25501 accagccgcc agcaacaaac attgcagatt cttgaaatgc tttctcagca tcttgtgcac
    25561 cgtagaaatg tacaatatct gagtcgatca tttctttgat taactttgca gcatgtttcg
    25621 cttctttagt attcagacgg atatttcctt cttcatcaag caaagattga ttctgctggg
    25681 ctagcatgtt gataaacaat tcttggtccc cagattctgg caaggcaatt ccaaaactac
    25741 cagttttttc ttttaatgct tttgccacct ggatatattc ttcccaagaa ctgatttctt
    25801 caaaatctac acctatttca gcaaattttt ttggttgata ataacaaagt gctggggtaa
    25861 aatcactagc ccaaggaata atcgtttcat tactcgtttg ttgacgcaaa atattcaatt
    25921 tttgatcata aaactgatcg ccatatttgt cataaaatcc atattcatca gcactataaa
    25981 aagcatcttg aaatttttcc agcacacctg ctgctgaagt gtctgccatg gagataatat
    26041 ccggcatatc ttcgtgtgtt accaattttg gggtgatcat ttgtactgtt gctgttcccg
    26101 aagctacatt cacaattttt aagtcaatat ttgctccagt tttgcggtta tagatttttg
    26161 cagcattttc aacatagttt gtcagtttgc cgtttgcgac atatacttct aatgtagtgg
    26221 ctttttttat cattggatct gcgatgtttg ctgttacttc ttcgttagtt tttccacatg
    26281 cacttaagct gaataatagg gtaatgttta gcaaagctat caccaatttg tatctttttt
    26341 tcatgtctat cccttctttc ttttcaagtt cttatataaa acgctttcac ttatgtatat
    26401 taacggaatt agtaacacaa atatttatat aaaaaaatat tttacttgtc gttttggctt
    26461 ttcatttttg ttataatagt cattaattat aaatagaaag ctttttatat aaaaagagaa
    26521 aggaaagtgg ttcttttgtt aacaaacaac caatcctcca atcttgtcaa aaaaacacta
    26581 acctattcca tagataccca tcttcattta tctgctgctc cctattttcg actactaata
    26641 aatgaaagcg ctaatttata tgctgaattt gcacataaat ctatagaact agataatcct
    26701 tcccttttat tcattgataa acagcaagaa gtccaaatct tttctactca aaaggaaaca
    26761 caaactttta tcattatcca gttttatttt gatgagcaat tattagcaca tattacggga
    26821 caatcttttt ctatagatca agaaataaca tctatcatta cttttctgaa aaatgaacag
    26881 ttcaaaattg attatctaaa acatagtaag ccagctattt cctatgatca ggaaatcaat
    26941 tcttttttca tactatgcca acagatgagc attcatttgc tttccttatt agttacttat
    27001 tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt atcaacagga acttgcttta
    27061 ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa agcatattct ttattcagat
    27121 gaattagttg atccaatcat acgatattta gaagaacatc tttcagaaaa ttttttgctt
    27181 gaacaattag cccaaatttt ttttatcagt ccagccagct taaaaaaaca ttttagaaaa
    27241 gtgaatggaa aaagtgttat tagctatttt aaagaactaa aaatggaaaa agcaaaacgt
    27301 ctcgtgctag aaaatgaatt aacctataca gaaattgcag atatattagg atttcattcg
    27361 atccatcatt tttcttcagc ttttaaaaaa tatacagggt tatccccttc aaagtattat
    27421 ctatccttaa aatagaaaaa aatttttagg ttttatccac aaattatttc tctatatttt
    27481 ctgtctattc aaaataaaca ataattttcc ggcacaatta tttgctgttt tttaccataa
    27541 catagtacga tcaacgtaga aaaggagatg tttttatgaa agttgttgtc attggcggtg
    27601 tagctggcgg tccttcattt gccactcgtt tccgtcgatt gaatgaagcg cacgaaatca
    27661 ttatctatga acgcggagaa aatatttctt atgcaagttg tgctttgcct tattatttag
    27721 gaggtgtgat cactgaccgt gactcgctga tcgaacgtac accagaaata ttgaaaacaa
    27781 aaaacaacat cgatgtcttt actaaacacg aagtaacagc aatcgatcct tctactaagc
    27841 gattaacagt taaagaccta tccacaaatg aagaaataaa aacagattac gataagttga
    27901 tcatctcttc tggtgctaga ccagattatc cggatattcc cggagttttt gaagcagaaa
    27961 acggctttgt cctccgtagt gtgacggatg cggatcgaat caaatcgttc cttgaagaaa
    28021 aaaatccaca acatgtcgtc attcttggtg caggtgttat gggtctggaa ttagctgaga
    28081 atttcaagca tcgcggctta gacgtgactt taatcgatca attgccacaa gtcgctttcc
    28141 cttatgatcc agaaattgct aatttagttt atgacaaatt gctgaaagaa ggattagccg
    28201 ttcatttaga aacaagagtt actgagatcc gtgataaagg tcgagaaata gtattatcag
    28261 atggctccgt cctttctgct gatatgctaa tttttgctgt tggtgtttct ccgaagaatg
    28321 aagtggtgaa agcaaccggc atacaattat cagatacagg tcagatcatt gttgatgacc
    28381 agttacaaac caatctgccg gacatctatg cgattggcga tattatcgaa acgactagtg
    28441 tagtgactgg tcagccgatc caaagtatgc tttccagtgc ggccaatcgt caaggacata
    28501 tgctggcaga tattttaaat ggtacgccta tgcgctatcg cggatatatt ggtgcaggtg
    28561 tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat gacagaacat gaattaaaag
    28621 catcaggcat aacaaattat aaaactgttt ttatcactcc ctttgaccat gcctatttct
    28681 atccagaagc tacaagatta aatctaaagc tgatttttga tgcagatagc ggtcgtattt
    28741 taggtggaca agcatttgga gaaaaaggtg tcgataaacg gatgggagaa ctttctgtag
    28801 caatcaccgg aaacttgaca gtctttgatt tgcctgattt ggagttgcct tactctccac
    28861 cgtattctac tacccgtgac ccgttgaata tagctggtta tgtcgcaatc aatcaaatga
    28921 cgaatatcgt agaaacgatc aaagcaagtg atatacccga aaacgatttg aaagaagcgt
    28981 tctttttaga tatacgtgaa cctaataaag caccttctgg cagtatctcg gcaacaaaaa
    29041 atattccaat gaatgagtta cgagatcgaa tcaatgaaat cccaaaagat aaaaaagttt
    29101 acattacttt cagaagagga ttgaatactt atacttctgc ccgaatcttg gcaggtttgg
    29161 gtatcaaagc gattttgatt gaagaataaa aaaacgaccg cggtgggtag ccgcggtcta
    29221 ggagttattt ttacttgagg agtaaaaatg aaaaagtata tagggttgtt ggttggtatg
    29281 tatatagaat agaagataaa tgtgaagaaa ttgtgcatta tttcttcaca tttattaggt
    29341 tatagtttaa gaaagtttaa acattgcgca atttaattat tttagctgtt tcttttcata
    29401 aaaaaacatg ttgtgactct gagacaaatc atagtccata gtgatgtctc tagagtaact
    29461 acgcctaata ttacgaggca tcatattaat ctatcagcac tagtacctaa actagcatcc
    29521 ctttcgaata ttttttctta tataataaag tttgctttca ctcctctaat ctcaccaaat
    29581 cgactggctt gcgttgtgtg atactaggaa ctgattggtt tagaacgact tttctattgt
    29641 aatcatattt ttcgccatcc tcgttgtctt ctaatccttc aatgaatgat ccctaatgtg
    29701 tgtattaaat aatttaattt ttttgttcct atttatttaa
//
LOCUS       DACCDF010000031        27999 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCDF010000031 DACCDF010000000
VERSION     DACCDF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.031"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(5..586)
                     /locus_tag="IVK51_001361"
     CDS             complement(5..586)
                     /locus_tag="IVK51_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6430964.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IVK51_001362"
     CDS             complement(769..1395)
                     /locus_tag="IVK51_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP6430965.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IVK51_001363"
     CDS             complement(1417..2748)
                     /locus_tag="IVK51_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP6430966.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IVK51_001364"
     CDS             complement(2903..3304)
                     /locus_tag="IVK51_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP6430967.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IVK51_001365"
     CDS             complement(3390..3596)
                     /locus_tag="IVK51_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430968.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IVK51_001366"
     CDS             complement(3691..5193)
                     /locus_tag="IVK51_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP6430969.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVK51_001367"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVK51_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP6430970.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IVK51_001368"
     CDS             complement(5764..6381)
                     /locus_tag="IVK51_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430971.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IVK51_001369"
     CDS             6602..7975
                     /locus_tag="IVK51_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6430972.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IVK51_001370"
     CDS             8050..9162
                     /locus_tag="IVK51_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP6430973.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTVHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IVK51_001371"
     CDS             complement(9234..10871)
                     /locus_tag="IVK51_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP6430974.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IVK51_001372"
     CDS             complement(11039..11647)
                     /locus_tag="IVK51_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6430975.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVK51_001373"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVK51_001373"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP6430976.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IVK51_001374"
     CDS             complement(13201..13953)
                     /locus_tag="IVK51_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430977.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IVK51_001375"
     CDS             complement(14094..15044)
                     /locus_tag="IVK51_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6430978.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IVK51_001376"
     CDS             complement(15045..15884)
                     /locus_tag="IVK51_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6430979.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IVK51_001377"
     CDS             complement(15881..16627)
                     /locus_tag="IVK51_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6430980.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IVK51_001378"
     CDS             16865..18235
                     /locus_tag="IVK51_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6430981.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IVK51_001379"
     CDS             complement(18290..19186)
                     /locus_tag="IVK51_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6430982.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IVK51_001380"
     CDS             complement(19467..20144)
                     /locus_tag="IVK51_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6430983.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IVK51_001381"
     CDS             complement(20235..21140)
                     /locus_tag="IVK51_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6430984.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     KNFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IVK51_001382"
     CDS             21315..22769
                     /locus_tag="IVK51_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6430985.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IVK51_001383"
     CDS             22785..23816
                     /locus_tag="IVK51_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP6430986.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IVK51_001384"
     CDS             23823..25010
                     /locus_tag="IVK51_001384"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP6430987.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IVK51_001385"
     CDS             25166..26548
                     /locus_tag="IVK51_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6430988.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IVK51_001386"
     CDS             26520..26906
                     /locus_tag="IVK51_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430989.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IVK51_001387"
     CDS             complement(27106..27774)
                     /locus_tag="IVK51_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP6430990.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         7795 a         5725 c         5145 g         9334 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggatag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactgttc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt ttgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DACCDF010000032        26539 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCDF010000032 DACCDF010000000
VERSION     DACCDF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.032"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1229)
                     /locus_tag="IVK51_001388"
     CDS             complement(99..1229)
                     /locus_tag="IVK51_001388"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP6430991.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDRLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVK51_001389"
     CDS             complement(1878..3080)
                     /locus_tag="IVK51_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6430992.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVK51_001390"
     CDS             complement(3140..3343)
                     /locus_tag="IVK51_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP6430993.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVK51_001391"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVK51_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVK51_001392"
     CDS             4880..5692
                     /locus_tag="IVK51_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6430994.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="IVK51_001393"
     CDS             complement(6716..6913)
                     /locus_tag="IVK51_001393"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6430995.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVK51_001394"
     CDS             complement(7059..7937)
                     /locus_tag="IVK51_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6430996.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVK51_001395"
     CDS             complement(7976..9094)
                     /locus_tag="IVK51_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP6430997.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVK51_001396"
     CDS             complement(9091..11190)
                     /locus_tag="IVK51_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP6430998.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVK51_001397"
     CDS             complement(11241..12062)
                     /locus_tag="IVK51_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6430999.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVK51_001398"
     CDS             complement(12059..12868)
                     /locus_tag="IVK51_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6431000.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVK51_001399"
     CDS             complement(12889..13383)
                     /locus_tag="IVK51_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6431001.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVK51_001400"
     CDS             complement(13395..13883)
                     /locus_tag="IVK51_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431002.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVK51_001401"
     CDS             complement(13960..16656)
                     /locus_tag="IVK51_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6431003.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVK51_001402"
     CDS             complement(17084..18157)
                     /locus_tag="IVK51_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6431004.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVK51_001403"
     CDS             complement(18154..18981)
                     /locus_tag="IVK51_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431005.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVK51_001404"
     CDS             complement(18982..19788)
                     /locus_tag="IVK51_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431006.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVK51_001405"
     CDS             complement(19800..20885)
                     /locus_tag="IVK51_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431007.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVK51_001406"
     CDS             complement(20902..21444)
                     /locus_tag="IVK51_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6431008.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVK51_001407"
     CDS             21702..22691
                     /locus_tag="IVK51_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP6431009.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVK51_001408"
     CDS             22688..23425
                     /locus_tag="IVK51_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP6431010.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVK51_001409"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVK51_001409"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP6431011.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTVIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVK51_001410"
     CDS             23989..24621
                     /locus_tag="IVK51_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6431012.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVK51_001411"
     CDS             24706..25353
                     /locus_tag="IVK51_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6431013.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVK51_001412"
     CDS             25358..26341
                     /locus_tag="IVK51_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP6431014.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8114 a         5606 c         4501 g         8318 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ctatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacggt catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccga
//
LOCUS       DACCDF010000033        26239 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCDF010000033 DACCDF010000000
VERSION     DACCDF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.033"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK51_001413"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IVK51_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP6431015.1"
                     /translation="MVSSIITALYLLNALIALVDILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IVK51_001414"
     CDS             1995..4661
                     /locus_tag="IVK51_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6431016.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IVK51_001415"
     CDS             5025..5777
                     /locus_tag="IVK51_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6431017.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IVK51_001416"
     CDS             complement(5799..6953)
                     /locus_tag="IVK51_001416"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP6431018.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IVK51_001417"
     CDS             7138..9579
                     /locus_tag="IVK51_001417"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP6431019.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IVK51_001418"
     CDS             complement(9909..10613)
                     /locus_tag="IVK51_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431020.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IVK51_001419"
     CDS             complement(10800..11045)
                     /locus_tag="IVK51_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431021.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IVK51_001420"
     CDS             complement(11062..11913)
                     /locus_tag="IVK51_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP6431022.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IVK51_001421"
     CDS             12079..13083
                     /locus_tag="IVK51_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP6431023.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(13324..13680)
                     /locus_tag="IVK51_001422"
     CDS             complement(13324..13680)
                     /locus_tag="IVK51_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6431024.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            14048..15235
                     /gene="tuf"
                     /locus_tag="IVK51_001423"
     CDS             14048..15235
                     /gene="tuf"
                     /locus_tag="IVK51_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP6431025.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK51_001424"
     CDS             complement(15423..18014)
                     /gene="mprF"
                     /locus_tag="IVK51_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP6431026.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(18040..18759)
                     /locus_tag="IVK51_001425"
     CDS             complement(18040..18759)
                     /locus_tag="IVK51_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6431027.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            19079..19600
                     /locus_tag="IVK51_001426"
     CDS             19079..19600
                     /locus_tag="IVK51_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP6431028.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            19659..20519
                     /locus_tag="IVK51_001427"
     CDS             19659..20519
                     /locus_tag="IVK51_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP6431029.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            20626..23019
                     /locus_tag="IVK51_001428"
     CDS             20626..23019
                     /locus_tag="IVK51_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6431030.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            23201..25042
                     /locus_tag="IVK51_001429"
     CDS             23201..25042
                     /locus_tag="IVK51_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP6431031.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK51_001430"
     CDS             25066..26175
                     /gene="rpoD"
                     /locus_tag="IVK51_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP6431032.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         8467 a         4669 c         5481 g         7622 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatatccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca aaaaaagagc gtgagacaaa agtaaagaac
    13201 acttttgtct cacgctctaa acacgtataa acggcggaag cagaagcaac tccttcttat
    13261 ttcttggagt taaacgcttc tgtcccagcc tctttttatc taataaaatt ttaagaccat
    13321 ttactaatta tcgatttttt ctgccgcatt tttaggcact tcagaagcct ttatttcttc
    13381 ccagcttaat acacctttac gaggattgac tttgagttta agatacgcat tcatctttaa
    13441 tggatgttct ctttcttcgc gaagcgtcac ctgttttttt tcaccatctt tattataagc
    13501 cgcttgcttg taggtataag tcgtgaattt ctctccgtta tctgcttggg tagctgacat
    13561 ttcgccattc gtcgtgattt ttgtataata agcttcgccg ccgtaaaaat aatgataagc
    13621 tgcaaaactt ccgccgagaa atatgcctaa aacgacaagt acgccaatta attttttcat
    13681 gtacttcacc tctttcctta ttattaacga aatagaagat ttcgtctatt gaaagttgtt
    13741 ggcaacagct gaattttaaa cttttcctca ttttgttctt attgcttcaa aagaaagtta
    13801 aggactatct tgaagttttt ctcaaaaaac acattttttt atttataaaa ggtacttttt
    13861 tcgcgtataa gacagacgag tgctcttagt tgactttgac caatattgac gtataataag
    13921 cttagttcga atcttacaaa aagtaagtcg gacgtattct ttttgattta gtcaaaaaaa
    13981 gactataata aaaaggtgag agctgaaatt tttcagtgat ctatattatt taggaggttt
    14041 gtaaattatg gcaaaagaac attatgacag aagtaaacca cacgttaaca ttggtacgat
    14101 tggacacgtt gaccatggga aaactacttt aactgctgcg atcactacag tattaggcaa
    14161 aaaaggccta gcaaatcctc aagactatgc tagtattgat gcagctccag aagaacgtga
    14221 acgtgggatt acgatcaata ccgcacacgt agaatatgaa acagaaaaac gccactacgc
    14281 gcatattgat gcaccaggac acgcggacta tgtgaaaaac atgattacag gggcagcaca
    14341 aatggatggt gcaatcttag ttgtttctgc aactgacggt ccgatgcctc aaacacgtga
    14401 acacatttta ttgtcacgcc aagttggtgt aaaatacctg attgttttct tgaacaaagt
    14461 tgatttagtc gatgatgaag aattgatcga tttggtagaa atggaagttc gcgagttatt
    14521 gagcgaatat ggtttcccag gcgatgacac tcctgtgatc aaaggttccg cattaaaagc
    14581 attgcaaggc gatccagatg ctgaagctgc tattatggaa ttgatggata cagtagatga
    14641 atatatccca acaccagaac gtgatacaga taaaccatta ctattgccag tggaagacgt
    14701 cttctcaatt acaggtcgag gaactgttgc ctcaggtcgt attgatcgtg gtgctgttcg
    14761 tgtcggtgat gaggtagaga tcgtagggat caaacctgaa acacaaaaag cagttgtaac
    14821 aggtgtagaa atgttccgta aaacgttaga ttacggggaa gctggggata acgtaggcgt
    14881 gttgttacgg gggatccaac gtgacgatat cgaacgtgga caagtacttg ctaaaccagg
    14941 ttccattact ccacatacaa aattcaaagc agaagtgtac gtgttgacaa aagaagaagg
    15001 tggacgtcat actccattct tcaacaacta tcgtccacag ttctacttcc gcacaactga
    15061 tgttacagga acaatcacat tgccagaaga tacagaaatg gtcatgcctg gtgacaacgt
    15121 gacgattgat gttgacttga tccatccaat cgctgttgaa aacggaacga cattctcaat
    15181 tcgtgaaggc ggacggacag taggttcagg tatcgttaca gagatcgaag cataagaaac
    15241 gcgactcgaa tattttggta atgaaaaatg tttaaaataa aagagcggtc caaacaggat
    15301 ttctgtttgg accgctcttt gttcttcaat tgtataagaa gagattgtag aagcatgaac
    15361 tttcgtttta cttcagtctc ttttattcat catgagagtg ttcatctatc ggtatgcagt
    15421 ttttaatact ttcttcgtat ccattttttc aaaccactta ttctaccttg tgtctgctca
    15481 actggcgcat tatcaataat cagcaaagca atcataacat atgcaatcca actatctcta
    15541 gaatatagag tataacgtga gacccagttt gtagcgtact tgcttttata ttctcttaat
    15601 ccatggaaag aataaaaatg agaaccaaac tcgtaaacca agtatgcaat cctttcctgt
    15661 aaaaaactct ttctggaagt tccgacattt gaaagagggg ccatccctaa attgaaccat
    15721 tggatatgtt cttctttcat ataggcaaac aaatgcaaga acaagaaatc catactgcca
    15781 gaaggtgctt ttttagaatc atatcgcatc agatcaatcg ttcctacttt attgttcgtg
    15841 taagtaggca tgatcgttgc aaaagcgaca atttcttctt gggtattttt gacaactgca
    15901 ataggcgctc tttgtagata atcttcagag aaaaagccta aagaaaaccc tttttccttt
    15961 cgtgaaccca accaattatc agagatattt ttgaaagtac tcatttgctc agtagaaaat
    16021 ggtggttgta agacatcaaa tgtaaatcct tctcgttcga tccgattcaa gacagcacga
    16081 gtgcccttca tttttttccc tgaagttgta aaactattca ggtctacata agcttcttcc
    16141 cccatcttaa tgaaatcgta gccgaattca tgcaaaatca taacaatctc ttcgcttgtt
    16201 tcgtagaaaa ctggtaaata gcatagtcga tctgtttctt cgataaaagc ttcgattgct
    16261 tctggaaaat cttctttctt accagaagga tcacccatta caatacattt attattgaag
    16321 caagcgaatt ggagaagaac agttggttca ccgtcttttt cataggcaaa catccgctta
    16381 tcttttaaga agatcaattg actatcggag tttcctccat aagttgtaag gatcttcagg
    16441 gccttttctt cattaaaggc ttcgccaatc tgtttctttt ctccttgaag gaaatgaaca
    16501 aatagaacga tcatgaaact aacagcgatg atggccaaca atcccgaaaa ccaaatcttt
    16561 tcagaaggaa ataagaaaaa ggaaataaaa tggtgacgcc gatgagggaa atccggcaga
    16621 ttgtataccc caataacgat gtaaagcaag gacagtgcac caataatgat tccatcgatc
    16681 gtacgccact cccatgaata gatcaattgt tcccgaaata attcgttttt agaagcaaga
    16741 atgatcagaa gcagaataga caagaaaacc accggtgtga aactaaaatc acttaataaa
    16801 acataaagta aagctagaac aatcaagaag attgtaggca aatatgctcg tttcacacgt
    16861 gctgcgatcc ctctgcccat aatgattaaa agaaatccca atagtatact aggaaattgg
    16921 ataataaaat gaaatttcaa tgggttcaaa ctatgaagcc agcgaaactc tgtaaaagct
    16981 tgcgggatag tggcagaaag tactagcatg attccagaaa agtataataa gacaaccacg
    17041 attttatgcg caatttctgt tgctaattgt ttagggatac cagaataacg ctgatcaaaa
    17101 gtggaaccga tatttttgaa gaaaaagacg attccaatca aaaacggaat gatgtagtag
    17161 aacaggcgat agagcaagat ccacacaacg actatttctc ttggcacacc aatagcggac
    17221 aacccgataa tcatcattac atcgaaactc ccaatttctc ctggaatcat agacacgatt
    17281 cctatcactg aagcagcaat gaataatgga attgtttgcc acagatcaat ccggatttcc
    17341 attaaataac caacagaaag gaaactaaac aggacaccgg tccattccaa caatgaaact
    17401 agcaatagct gtccttgagt tttaagtgag gtaccaccaa ggaatccttt ctttttgaaa
    17461 gtagtaaata aaaagacgac aggaaaatat agaccgccgc cgatcaacca tatccaatat
    17521 tgcttcaaaa aaggagcaac tggcgtgaag actacaagta aaaaagaaag cagactgtaa
    17581 atagaaagtc cagccatcaa aaacaagaaa actttcgata atgcttggat cacgtttctg
    17641 ctctctttct tttgtccata tagttcagag cgaagcccca cactaacaaa cccgccgaaa
    17701 ccggcaatat tgttgatcgt attgatcagc cagcttgtct caaacaggta acgaggattc
    17761 tgcttttgtt caagcatccg attcaaaata atatcataac caaccatagg taacacagag
    17821 ataagaccaa tcaatagcat tagtccgctt ttccatacag gtatagtggc aaacgtttct
    17881 cctaattgct ggatagatag agttttaccg atggacatca attcaccgac aataatgaca
    17941 atgacagaaa tcaaaaagat tgttttgaac agtcctaggt ggtttttcag ccattgtatg
    18001 gtatttttta acaaataaat caccttctat tcctaatagt tatgtttcag aaagaaattg
    18061 aagaacttct cgattgaagc gttcaggatc ttttctagct aattcatgtc cttgttcttt
    18121 cacaagcaca aaggaagctt ttggtatagt tttagctata tacaaagaat gttttagctt
    18181 gattacatct ttttttccca cgatgatcaa agtgggggag ttgatttttt ttaaatcatt
    18241 ttctgtaagc ccgatgtcat gcagcaaaag tttgataact aaaagatttt tgcgaagact
    18301 tgggcgaaat aaagagagta accataccca tgcataatga atattactga tgactctggc
    18361 agaaaaacga acgcctttaa caagcgtgtt tccagaattt aaaatcaatc gatgaacttt
    18421 tttagggaag ctgctggcaa ataccagtgc tagatttgct ccatcactaa atccaagaaa
    18481 atctgcctga tcgatttttt ccagcaacat gatcgtattc agatcctcgg ccattaactg
    18541 aaaattcaac atagatgctt cgtttgtcga acgaccatgt cctcggctat ctactagata
    18601 cacggtatag tagcgttcga gtacagggac ctgttctgaa aaaaagcggc cgctgccgtc
    18661 attgccatgc agtaaaaaga gagggaagcc ttgaccgctc ttttcatagt aaatttcgct
    18721 cccatctggc atttttgcat atttcttttc ttttttcaca taatcaccta ctgtgtaaag
    18781 tataacgatt atatcagtca ttgggcaatt gtttttactg aaaaacaacg atcattttcg
    18841 taagatgtca aaaaatacag gaaaattcat tttttcttaa ggcgtacaca gaaatcaagg
    18901 aagaaatagc caagaagata aagaaatagt aagataaaaa aagatgtcaa aataactagg
    18961 tgatgaaatg aagcgaaaac gattatctat tatccgtatt cttttttaca tgatcattct
    19021 gacggttatt ggtgcggtgt tgtttttatg aggaagaact tatcgttcgg taaaacgagt
    19081 ggaaagctat caaatagaaa taacaacagc tgcaaaaaat tatcatatgg aaaactacga
    19141 gaatttgatc aaggcgataa tattgaccga atcaaaagga aaagggacag atctcatgca
    19201 gagctcggaa agtctgcatg gagaagctga gatcattgat aatccagctg acagtattga
    19261 tcaaggtgtc aaatatttgg cggaaatgat agtagaagca aagaaacaag gatgcgattt
    19321 atggacagct gtccaagcat ataattttgg gaaagattat attgcctatg taaaaaatca
    19381 tggcggagaa aacacagtag aattagcaga agcgtattct aaagatgtgc tgagcccact
    19441 tttaggaaat gaggaaggga aacaataccg atattggcgg atccagtcct tgctttacaa
    19501 tggcggctat ctctatcata atggaggtaa catgttttat cctgatattg tcaaaatgaa
    19561 tgaaaaaata atagagacct atgaaaaagt ctttaagtaa aatagcagga aatgttcaat
    19621 catgaataaa attaaatgaa agaaggagtg cgcaaatagt ggaaaaaagg caaaaaatgg
    19681 aaaatcaaga agtggggtac acaaccaaag actggatgtt acggtttgtg aagggaatgt
    19741 ttatcggttc aggatttatc ttgccaggag tcagcggtgg tgcattagca gctgtctttg
    19801 gcatgtatga gcggctgatt tcttttttag cccaccttac aaaaaatttc aaagaaaatg
    19861 ttttgttttt tctgcctgtt ggtctaggcg gagtgacagg catctttcta ctttcttttt
    19921 tagtcagttt tttattaggt gcgtatgaaa caacgatatt atggtttttt gtcggatgta
    19981 tcgctggaac tgttcctgcg ttatggaaag aagcagggaa aaaaggaaga gatacttctg
    20041 atgtgatcat catggttgtt acttttgttc tggcttattt gtttttatta tacggcgctc
    20101 aattatttga cgggcaagtc gatcagaatt ttggaacatg gctgattgcc ggcggattga
    20161 ttggtttggg tatgatcgtc ccaggattga gcccttctaa ttttttggtg tatatgggat
    20221 tgtataaagc gatggctgac ggatttaaag aagtacgact tgaagtgatt gtgccgatcg
    20281 gaattggtgg gattctttgt gttcttggat tgtcaaaaat catggattac attttctcaa
    20341 aggcatatag caagctattc catttcattt taggtgtagt ttttgcttca acgattatga
    20401 tcattcctac cgattacgcg ggattgggag ttgctggggt actagcttgt cctgttcttt
    20461 ttatagccgg tacagcacta ggttggtgga tgagccgttt ggaggaacaa tataagtaaa
    20521 gatagattga taataaaaac agcacacggg tgattcaaac aaaaaggttg gcatcttttt
    20581 tgtaaaatca ttcgtgtgtt tttctataaa ttttataaaa acaagttgtt tttaatcttg
    20641 gaacacgcta gacttaaaca cgtgattatt tttgaaagtt ggcggaaaat ggagaaaaag
    20701 aaaatgaatg gacttacaca gcaagaggta gttcagaaaa tggcttctgg ccagcaaaat
    20761 gactaccagg aagatgcatc aaaaagtaca aaacaaatat tcagcgataa tatattgacg
    20821 ttattcaatt ttctgaactt tggtattggg atttgtctgt tatttgtagg tgcgtattcc
    20881 aacatggcgt atttagcaat tattgtcgtc aatatcgtta ttggaattta tcaagaaata
    20941 catgcaagaa atcttgtacg gaaattatcg attgtttccc aatcagaagt actagtgatc
    21001 cgggacggta aagaacaatc gatttctact aaagagctgg tattaggaga tttggtaaaa
    21061 atcggagcag gagaacagat accagccgat ttacaggtta tatctggacg ggcagaagca
    21121 aatgaagctc ttttgacagg cgaatctgat ttgattgtca aagaaactgg agctgaattg
    21181 ctttctggaa gctttttgac aagcggttct gtcatggctg aagttatccg agtaggtgca
    21241 gataattatg ctgtccgctt gactagcgag gcaaagactc acaaagctat tcgttctgaa
    21301 cttgtagatt cgattcgcaa agtatccaaa ttcactagtt tcgtgattat tcctttgggg
    21361 atcatactat ttttggaagc actgttcatc cgcgatgctt ctatcaaaat ttctgttatt
    21421 gcatctgcag cagctttgtt aggcatgctt ccaaagggtt tggtattgct aataagtatt
    21481 gcattaacga caggggtaac caaactggca aaaaaacgaa tccttgttca agacatgtat
    21541 tcaattgaaa cgctggccca tgtcgataca ttatgcttgg ataaaacagg gaccattact
    21601 gaagggaaaa tgagtatcca gcgggtagaa gcactacatc cgctgtacga gaaagccatc
    21661 ccagaaatta tggggacgta tctggcagaa agcagtgata acaacatcac tatgcgggct
    21721 atgagagatt attttgagct ttctaatcgg tatgatgtga caaacaaagt cgcattttct
    21781 tctgatcgca aatggggcgc gatggaattt ccagaactgg gagtcgtgta cgtaggagcg
    21841 cctgaacgtt tggtggaaga agatcggctt ccggacagtc taaaaacagc ccaagcgaat
    21901 ggctatcgcg tacttatgtt ggggatcgct ccaaatcagc aattgaacga agaaaaacca
    21961 gcatttgttg agccggttgc tctgtttgaa atcgatgatc caatccgaaa aaacgctcaa
    22021 gaaacactag cttatcttca tgaggaaggt gtggatctga aagtgatttc tggagacaat
    22081 ccttttactg tctccaatat cgctagacgt gcaggattac cgaattatga tgcgtatgtc
    22141 gatctttctc aaattactga agaaatggaa gtgcgagaag ctgctcatcg ctatacggtt
    22201 tttggacgag tgagtccgca gcaaaagaaa ttgctggtaa atgaattgaa agaaagcggc
    22261 agaacggttg caatgacagg cgacggtgtg aatgatgttt tggcgttaag agaagcagat
    22321 tgcagcattg ctatggcaga aggtgatgga gcaacacgtc agattgcgaa tctggtattg
    22381 ttggactctg attttactac attgccagaa gtcctgtttg aaggacgccg agtggtcaac
    22441 aatgtgacga aagtctcagg catctttttt atcaaaacga tttattcatt tattttatcg
    22501 attatatgtg ccgttacagc aattggattt ccgtttatcc ctatccaaat cacgctgatt
    22561 gatttagcga ttgaagggta tccttccttc tttttatctt ttgaacaaga taaaagaaaa
    22621 atcacttacc gttatttgcc tacagcactt attaatgctt taccaaatgc cttgcttgta
    22681 gtagtgaata tcattgcggt gtatctactt ggaaaaacat tcgcatgggg aaatgctgaa
    22741 actactactt tgatgtatta cttgctgatt gggatcagtt gcatggcagt tgtaaaagct
    22801 tgttatccct tcaacccatt gcgcatcttc ctagcagtaa cgacgatcgt agggatatat
    22861 gtagctgcaa tgctgttcca tggaattttg gaagtaaact tagggttggg agagagcgta
    22921 ggatacttct ttgtattcat gctcgtaaat attgttcttc gagtattgta ctggaaggct
    22981 gatattcaaa atcagttact gaaaagagta gcaaattgat gcttaatgag atagaaatgt
    23041 gttatacttg aaaaagcaat gaaatatgat acaatcaaac atgtataatt gtgtccttta
    23101 aaatcgcact gatttcagtt agtgcgattt tttaccggtt attctcctga aatatcttct
    23161 gttaaaccca aaagatatca atagagcagg gggtgaaaaa atggctcaac ggattcctca
    23221 agaggttatc gaaacagtac gcagtcaaac gaatatcgtt gaggtgattg gacaatatgt
    23281 ccagttgaaa aagtctggta aaaattactt ggggctgtgt ccttttcatg aagaacgtac
    23341 accgtctttt tctgttgcag aagacaagca gatttttcat tgctttggtt gtggtaaagg
    23401 cggaaacgtg tttacttttc ttcaagaact agaaggtcta agttttccag aagcagtggt
    23461 aaaagtggca gacttcgaac agataccgtt agaagatcaa tggcgtcagc gtactatagc
    23521 tgtccaaaat cctgaatctg ctacggggaa actgattgct gctcatgaaa aagcagcgga
    23581 aatctatcat cacatgctat tgcatacaaa agctggacag ccagctttag aatatttgca
    23641 aagtcgtggg ttgtctttag aattaattga agaattcaac ataggttttg cacctaatga
    23701 gcgttctttt cttcagcaag tattcaagaa tgaatcaatt tcaaatgaat tgttagaacg
    23761 ctctggattg tttgtcacgc atgatgatgg ccgtatgtct gatcgttttt atcaaagagt
    23821 gatgtttcca cttcgtaatt ctcaaggcca gacgatcggt ttttcaggac gctggcttgc
    23881 acctgaaaaa gggcaggaaa aagatcagcc caaatacttg aatagtccag aaacagaact
    23941 gttcaataaa agacaagttt tatttaatct tgataaagca agaagcgata tccggaaaaa
    24001 cggagaaatt ttgttgttcg aagggtttat ggatgtgatt gctgcatggc aggctggtat
    24061 caaaactgga ttagcttcta tggggacaag tctaacagct caacagatcc aacaaatgga
    24121 aaagctgacg aaagaattgg tcttttgcta tgacggagac aacgcaggtt ttgaagcaac
    24181 caatcgaggg attgaattgc ttcggcaaaa cagtcgatta gcactttctg tcgtagtcgt
    24241 tccagaaaag cttgatcctg atgaatatct ccgtaagtat ggagaagcat ctttccaaga
    24301 attagtggct catgggcgcg aaacagtctt tacttttaaa tcacgttatt taaaacaagg
    24361 gaaaaattta gataatgaaa aagacaagat cgaatacctc gaagaattac tagttgaact
    24421 tagccgagtt gtttcaccaa tcgaacaaga tatgtatcta tctcaactgt caacagagtt
    24481 tcagatcggc agagagacga tacaaaaaca actgcgcgat cttcgaagga caaatcagca
    24541 aaatcgtcaa gaacctcagg aaagtcaaca cactcaggtt acgagacaag aaactgcacg
    24601 gaaaaagaga cctttgacgc aagtagaaaa ggcagagcag attctattat atcgtgcatt
    24661 taaagaagtt tctgtccgta atattttaaa agaacgagaa tttcagttta ttcatgatgc
    24721 atatgcagaa gtttattttt tattcgatgc atatgtctca caacattatg aattcaacct
    24781 tgcagagttt ttagactttt tgcaagatga aaatttaagg cggttggtgg tggaaatcga
    24841 atatttaccg gtggcagaag aaagttcgcc acgagaaatc caagacttgc tagatgtgat
    24901 cagcaaatct ggcttagctg atgaaataac attaaaaaaa caaatgcagc aagaagccag
    24961 acgaactgga aacaaacagc ttgagcttga attgacgatt gaaatcatca atttaacgaa
    25021 acagttaaaa caagccaaat agctactcaa ggggggcctt cttttatggc aaatggaaca
    25081 gataaaaaaa cgtatgaagc agcagtagca gctttcatca aagaaaacaa acccaaacga
    25141 caagttgtat atgatgattt atccaatcag cttgctacac cattcacttt gaatgcagat
    25201 gaaatggaca aattgatcca aaagattgaa gacgcaggta tcagtgtggt ggatgaaaat
    25261 ggtgatccat caattcacag cttgaagagt gctgagaaaa aagctgaaca agcaaaaaca
    25321 gaagatcttt ctgcgccaac cggtgtaaaa atcaatgacc cggttcgtat gtatctgaaa
    25381 gaaattggcc gtgtctccct tttaactgcg gaagaagaag tagcgttagc attgaaaatc
    25441 gaagaaggcg accaagaagc gaaacagcgt ctggcagaag caaaccttcg tttagtagta
    25501 agtatcgcta aacgttatgt aggacgaggc atgcagttcc ttgacttgat ccaagaagga
    25561 aacatgggac tgatgaaggc agttgaaaaa tttgactacc gcaaaggatt taagttttca
    25621 acctatgcta catggtggat tcgtcaagcc atcactcgag caatcgctga ccaagcacgt
    25681 acgatccgta ttccagttca tatggttgaa acaattaata aattgatacg tatccaacgt
    25741 caattgctgc aagaccttgg aagagaacct acgccagaag aaatcggtgc ggaaatggat
    25801 cttccaacag aaaaagtccg agaaatcttg aaaatcgcgc aagaacctgt ttcccttgaa
    25861 acgccaatcg gtgaagaaga tgactctcac ttaggtgatt tcatagaaga ccaggaagca
    25921 acaagtccag cagaacacgc agcttatgag ttactaaaag aacaattgga agatgtacta
    25981 gatacattga ctgatcggga agagaatgtc cttcgcttac gttttggttt agatgatggc
    26041 cggacgagaa cattggaaga agtagggaaa gtatttggtg ttactcggga acgtattcgt
    26101 cagatcgagg ctaaagcatt aagaaaattg cgccatcctt ctcgttcaaa acaattaaaa
    26161 gatttcttag aataagcgtg gactattgac cagaagagaa atcagtaaaa ctgtttggtt
    26221 tattttaaaa gaaaaaaag
//
LOCUS       DACCDF010000034        26027 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCDF010000034 DACCDF010000000
VERSION     DACCDF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.034"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK51_001431"
     CDS             complement(43..681)
                     /gene="plsY"
                     /locus_tag="IVK51_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP6431033.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            808..1242
                     /locus_tag="IVK51_001432"
     CDS             808..1242
                     /locus_tag="IVK51_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP6431034.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1408..3453
                     /gene="parE"
                     /locus_tag="IVK51_001433"
     CDS             1408..3453
                     /gene="parE"
                     /locus_tag="IVK51_001433"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP6431035.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3465..5915
                     /gene="parC"
                     /locus_tag="IVK51_001434"
     CDS             3465..5915
                     /gene="parC"
                     /locus_tag="IVK51_001434"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP6431036.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6192..8423
                     /gene="pflB"
                     /locus_tag="IVK51_001435"
     CDS             6192..8423
                     /gene="pflB"
                     /locus_tag="IVK51_001435"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP6431037.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8667..9428
                     /gene="pflA"
                     /locus_tag="IVK51_001436"
     CDS             8667..9428
                     /gene="pflA"
                     /locus_tag="IVK51_001436"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP6431038.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9513..10439
                     /locus_tag="IVK51_001437"
     CDS             9513..10439
                     /locus_tag="IVK51_001437"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP6431039.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10621..11514
                     /locus_tag="IVK51_001438"
     CDS             10621..11514
                     /locus_tag="IVK51_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP6431040.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11648..11773
                     /locus_tag="IVK51_001439"
     CDS             11648..11773
                     /locus_tag="IVK51_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431041.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11907..12539)
                     /locus_tag="IVK51_001440"
     CDS             complement(11907..12539)
                     /locus_tag="IVK51_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431042.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12687..13229)
                     /locus_tag="IVK51_001441"
     CDS             complement(12687..13229)
                     /locus_tag="IVK51_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP6431043.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13364..13753
                     /locus_tag="IVK51_001442"
     CDS             13364..13753
                     /locus_tag="IVK51_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431044.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13757..14521
                     /locus_tag="IVK51_001443"
     CDS             13757..14521
                     /locus_tag="IVK51_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6431045.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14650..15597
                     /locus_tag="IVK51_001444"
     CDS             14650..15597
                     /locus_tag="IVK51_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6431046.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15727..16467
                     /locus_tag="IVK51_001445"
     CDS             15727..16467
                     /locus_tag="IVK51_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431047.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16758..17426)
                     /locus_tag="IVK51_001446"
     CDS             complement(16758..17426)
                     /locus_tag="IVK51_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6431048.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17560..17847
                     /locus_tag="IVK51_001447"
     CDS             17560..17847
                     /locus_tag="IVK51_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431049.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18263..19801
                     /locus_tag="IVK51_001448"
     CDS             18263..19801
                     /locus_tag="IVK51_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP6431050.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19798..20763
                     /locus_tag="IVK51_001449"
     CDS             19798..20763
                     /locus_tag="IVK51_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431051.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20750..22072
                     /locus_tag="IVK51_001450"
     CDS             20750..22072
                     /locus_tag="IVK51_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6431052.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22135..22239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22313..23056
                     /locus_tag="IVK51_001451"
     CDS             22313..23056
                     /locus_tag="IVK51_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431053.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23071..24063
                     /locus_tag="IVK51_001452"
     CDS             23071..24063
                     /locus_tag="IVK51_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6431054.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24075..24812
                     /locus_tag="IVK51_001453"
     CDS             24075..24812
                     /locus_tag="IVK51_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431055.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24802..25524
                     /locus_tag="IVK51_001454"
     CDS             24802..25524
                     /locus_tag="IVK51_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP6431056.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25608..>26027)
                     /locus_tag="IVK51_001455"
     CDS             complement(25608..>26027)
                     /locus_tag="IVK51_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP6431057.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8954 a         4426 c         5428 g         7219 t
ORIGIN      
        1 atttgtcaca ctcccgtttt atctctttgg aatgaaaaag tatcaggatt ttttcttgct
       61 gctattcaaa ccaaacggga ttcgactttc agtatggttt ctgatacgct tgatattgtc
      121 tttatgccga taaaagataa aggaagaaac taaaaaggca atgatcgtca gtaaccaatt
      181 gggcttagcc aaaatcacag gagctacata aggaaggacg atcgtagata aagtgatcaa
      241 tactgcactg atcatactgc tcaaactcac catacttgta caaaataaac taacgataaa
      301 gatgatacaa gaataaacaa aaaatacagg attaaatcct aaaagcattc ctgcacttgt
      361 cgcaacagct tttcctcctt tgaacccagc aaaaatcgga aatgtatggc caagaatggc
      421 gcatacccca aaccaaagag ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt
      481 agccaatgta ccttttaaaa tatccataag caggacaaca atcccggctg ttttgcctaa
      541 tacacgaaat gtattggtcg ttccagtatt accgctcccg tgttgtcgga tatctttttt
      601 gaaaaacaat ttgccaatcc agacaccgga aggaatagaa cctaaaagat atgctaaaat
      661 aagtaataag atcaatttca ttttttaccc tctttcaagt tgaaaactat cctttatttt
      721 agcatgaata ggtaacagga acaattgaaa aaatgaaaga tacaagtata atagatggaa
      781 taaaaagaaa agcaaggggt ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt
      841 ttcaactatt tggaagaagc aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa
      901 cgaacaagtt acatggttgc acaggtattg caagacaatg gatataaaat cataccagta
      961 aatccattat tggctggtga agaaattcta ggcgagaaag tctatgcgaa tttgacagaa
     1021 atcgatgatc caatcgatat cgtagatgta tttagacgga gtgaattttt acctgaaata
     1081 gcaggtgatt ttttgaaaac tgatgctaaa gtgttctggg cacaattagg gattgaaaac
     1141 aaagaagcag ctgagatgct aagaaaagca ggacgtacag atatcgtaat ggatcgttgt
     1201 attaagattg aacttggtaa gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga
     1261 gatgatcgat agaggggaat gtcgaaaaag cggttaggag ataattaaaa ttgatttctc
     1321 gtctaccctc ttttttttca cgaccttttc tagtatgata atcgtggtgc ggttttgccg
     1381 caaagtatta ttgaaggagc gtttgccttg gcaaaaaaag taaacaacga atacaatgac
     1441 tcgtccatcc aagtattgga aggattagag gcagttagaa aaagaccagg gatgtacatt
     1501 ggatccactg acagccgagg cttgcatcat ttagtctatg aaatcgtcga taatgcagtt
     1561 gacgaagcat tatcaggtta tggcagtgag attgatgtga ccatccatga agacaatagt
     1621 ataactgttg cagatagcgg acgtgggatg cctgtaggaa tgcatgcttc cggaatacca
     1681 acagttgaag tgatttttac tgtgctgcat gctggcggaa agtttggtca aggagggtac
     1741 aaaacttctg gtggactgca cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg
     1801 ctaattgtaa cgatcgttag agatggcgta gagtatcagc agaaatttaa aaacggcggt
     1861 aaacctgacg gtactttgaa aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc
     1921 cattttttac cagatgatac aatattcagt acaacgaaat tttcatatga aattttagcc
     1981 gaacggttaa gagaatctgc ctttcttcta aaaggcgtta aaatctctct ttctgatctt
     2041 cgtggagaag aaccagtcaa ggaaattttt cattacgaag aaggaatcaa agaatttgtt
     2101 gattatctga atgaagaaaa agatacattg acaccagttg tttatttttc aggagaaaaa
     2161 gaaggtatcg aagtagaggt cgcttatcaa tacaatgacg gttattcaga aaatgtttta
     2221 tcttttgtca ataacgttcg gacaaaagat gggggaaccc atgaagctgg tatgaaagcg
     2281 gcgatgacga aatcttataa tgaatatgcg agaaaagttg gtttgctgaa agaacgggac
     2341 aagaatctgg aaggtagtga tttcagagaa gggttagctg ctgtactttc tatccgtgta
     2401 cctgaaaatc ttctgcaatt tgaagggcaa acaaaagaaa aacttggtac gccagttgct
     2461 cgtaccgtag tagataacgt catcagtgaa caaatgggct tttatttgca ggaaaatagc
     2521 gaaatgagcc agatgttggt ccgtaaagca atcaaagcca gagaagcgag agaagcagca
     2581 cgtaaagcca gagaagaaag ccgaaatggg aagaagcgta aaaaaggcga atctcttctt
     2641 tcaggaaaat tgacacccgc acaatcccga aaccctaaga aaaatgaatt gtatttagtc
     2701 gaaggggatt ctgctggcgg atctgctaaa caaggaagag acagaaagtt ccaagcaatt
     2761 ctacctttac gaggaaaagt catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa
     2821 aatgaagaaa tcaatacgat gatctatacg attggtgcag gagtcggtcc tgagttttct
     2881 atcgaagact gtaattacga caaagtaatc atcatgaccg atgcggatac agacggcgcc
     2941 catattcaag tattactgct tactttcttt tatcgttata tgaaaccgct gattgaagct
     3001 gggaaagtat atatcgcgtt accgccttta tataaagtat ccaaaggaca aggaaagaag
     3061 caagtcattg aatatgcgtg gacagatgac gaattggcag caatgattaa aaaagtcggc
     3121 aaaggatata tgctgcagcg atataaagga cttggagaga tgaatgcgga acaattgtgg
     3181 gaaacaacga tggatccgac ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg
     3241 caagctgaac gtcgagtaac cacactgatg ggtgacaaag tagaacctcg aagaaaatgg
     3301 atcgaaaatc acgtgcaatt tacgctcgaa gaagatggca gtatcttaga taaaaaagag
     3361 gatactgaaa tctctccttc agtttctaat gatttattag atgaagaacg agcagataaa
     3421 aatgaaaaca atcagttatt tgaagtagaa taggaggtca cctgatggaa aatcgacaag
     3481 aagtacaaga attaacttta gaagaagtga tgggtgaccg cttcggacgt tattccaaat
     3541 atattatcca agaacgtgca ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc
     3601 ggatattatt tgccatgaac aaagatggca atacgtatga caaaggattc cgtaaatcag
     3661 caaaatctgt cggaaatatc atggggaatt atcatcctca tggtgaccgc agtatttatg
     3721 aagcgatggt ccgcatgagc caagactgga agttgcggga agtactgatc gaaatgcacg
     3781 ggaataacgg gagtatggac ggtgatccac ctgctgcgat gcgttatact gaagccagat
     3841 tatccaagtt aagtggagag atgctagcag atatcgagaa agaaacagtc gatctcgtgt
     3901 ggaattttga tgatacagaa aaagaaccaa cagtccttcc agctcgatac ccaaaccttc
     3961 tcgtcaatgg atcgacaggt atatctgctg gatatgcaac agaaatcccg actcataact
     4021 tagctgaagt gatcgatggc acaatctata tgatcgatca tccacaagcg agccttgaaa
     4081 aattgatgga atatattcct ggacctgact ttccaacagg agggatcttg caaggaaaag
     4141 atgaaatcaa aaaagcttat gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa
     4201 agatagaggc aatgaagggt aataaacagc aaatcgtgat cacagaaatc ccatatgaag
     4261 taaataaagc gaccttggtg aaaaaaatgg atgagatccg tttgaataag aaaatcgatg
     4321 gaatagctga agtacgagat gaaagtgata gaacaggtct gcaaattgtc gtcgaattga
     4381 aaaaagacgc caatgctcaa ggaattttga attatttgtt caaaaacact gagctacaga
     4441 tcaattacaa tttcaatatg gtcgctatcg accatatgac tccgcatcaa gttggactga
     4501 aagacatttt aagaagttat atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat
     4561 tcgatttagc caaagctcaa aaaagacagc atatcgtaga aggattgatg aaagctttat
     4621 cgattctgga tgaagtaatc gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa
     4681 agaatttagt cgacgtcttt caatttacag aagaacaagc agaagcaatt gtcacattgc
     4741 aattataccg tttgacgaac acggatatca cagagcttca aaaggaatcc gaaagcttga
     4801 ttgcgcaaat cacagagttg aataaaatcc tttcaaatga caaagagctt ttctctgtta
     4861 tgaaaaaaga attgcgagaa gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg
     4921 aagacgaaat cgaagaaatc aaaattgata cgcaagtctt agtggcacaa gaagacgtaa
     4981 ttgtcagtgt gacgcgagaa ggctatgtga agcgaacaag ccttcgttct tatagtgctt
     5041 ccaaaccaga agaaatcggg atgcgagaag gcgactactt gctatactca ggtgaattga
     5101 gtacattgga ccatgttctt ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc
     5161 atgaactacc cgatttgaaa tggaaagatg ctggtgagca catttctcaa acaatctcca
     5221 atctgtctgt agacgaatcg attttagctg ttttcccata tcaaaaaatt caagcagaga
     5281 aaacatttgt attcatcagc aagaatggat tgatcaagca aacacgaatg acagagttcg
     5341 aaccatggcg cacctataag agccgtccgc tcagctgtat gaagctaaag gcggaagaag
     5401 atgaactgat tgctgtgtat ctgaaagaag atcagccagg cttagatgtt ttcttagtaa
     5461 cgcatcaagg aatgggtttg cgctatcctt taacagatgt tcccgttgtt ggagcaaagg
     5521 cagctggagt gaagtctatg aatttgaaag atggagatag gattatcaac gggctgcttg
     5581 tgttagcaga aggtgatacg ccgatcgtaa tcgttactca gcgaggagct gttaaacgca
     5641 tgcttgctca agaaatcagc caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg
     5701 agctgaaaaa aaatccgcac cgtattatct atatgtcgga aggaagatca aaagaaataa
     5761 ccttgatgaa tcaaaaaggg cagcagttaa cgattgaccc aactgatttc cctataggtg
     5821 atcgaacatc gaatggatca tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag
     5881 ttatcgatgc tcctgagatt ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga
     5941 cgccatctgt attaatacag gtggcgtctc tttttcattc atttttaata aatccttata
     6001 cgtttgcaaa aaaagatgaa agtgttcaca atgcttaaat gttggtatgt caacgttgat
     6061 ttaaatatat gtaaataaaa cataaaaatt gttaaataaa aaacaagaaa acccttgcaa
     6121 agatagggtg ctgtgttata ctaatcacgt aaaaactgtt acataaaaca gaaatttagg
     6181 aggtccatac aatggagcaa tggaatggat ttaaaggtac caaatggaaa gaaggcgtag
     6241 acactagaga ctttattcaa aataactaca ctgaatatcg cggtgatgac agctttttgg
     6301 aaggcccaac agaagcaaca gaaaaattat gggatgaatt gcaaaagtta aatgatatcc
     6361 aattcgagcg taacggtgtc tatgatatgg acacagaaat cgtatcaact attacttcac
     6421 acgatgcagg ctatttggat aaagatttgg aaaaaatcgt cggtctacag acagacaaac
     6481 ctttgaaaca agcattcatg ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa
     6541 gcaatggcta tacaccaaac gatgaattga cacatatttt cactgactgg cgcaaaacac
     6601 ataatcaagg tgtatttgac gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa
     6661 tcattacagg gcttccagac gcttacggtc gcggacgtat catcggtgac tatcgtcgtg
     6721 tggcattgta tggaatcgac tatttgatgg aacaaaagaa aaaagatcat gatatgactg
     6781 gttatcatac aatgagtgaa gaagtgattc gtttacgtga agaaatcact gaacaatatc
     6841 gtgcattagc gcaaatgaaa gaaatggctg cgaaatatgg ctatgatatt tctaaaccag
     6901 ctgcaaatgc aaaagaagct gtccaatggc tatacttcgg atatttagca gcaatcaaat
     6961 cacaaaatgg tgctgcgatg tcaatcggac gtatctcagc attccttgat atctatatcc
     7021 aacgtgattt ggataatgga gtaatcacag aaaaagaagc acaagaattg atcgaccact
     7081 tagtaatgaa attacgtatg gtcaaattcg cgcgtacacc tgaatataac caattattct
     7141 caggattccc tatctgggct actttatcca ttgcaggtat gggattagat ggtcgttcat
     7201 tagttacaaa gaatgatttc cgtctgcttc atacattaac aaatatggga ccttcaccag
     7261 aaccaaactt gactgttttg tattctgaac accttccaga aggattcaga acatatgcta
     7321 gcagaatcgc tatcgaaagt tcttcgattc aattcgaaaa tgataatcag cttcgtgaac
     7381 aatggggttc tgatgattgt gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag
     7441 acatgcaatt ctttggcgct cgtgctaact tggctaaaac actattatat gccatcaatg
     7501 gcggagtcga tgaagtgact ggcgcccaag ttggaccaga atatcgtcca atcacatcag
     7561 aaatactaga ctttgaagaa gtgaaacata acttcatgaa tatgatggat tggttagcag
     7621 aactatatgt caatacgttg aacatcattc attatatgca tgataaatat tcttatgaag
     7681 cagctcagct ggctttgatg gattctgact tgcaacgcac atttgctaca ggtattgctg
     7741 ggatctctca tgcggcagac agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc
     7801 gtaatgaaaa aggaatagca gttgattttg aagtggatgg cgacttccct aaatacggaa
     7861 atgatgatga ccgtgcagat gaattagttt catggatctt ggaatacttc atgggacaaa
     7921 tcaaacgtca taaaacttac cgcaactcaa aaccaacaac ttcattattg acgattactt
     7981 caaatgttgt ttacggtaaa aatacaggaa atactcctga tggccgtgaa gctggaaaac
     8041 cacttgcgcc aggagcaaat ccaagttaca atgcggaaca aagtgggtta ttagctagcc
     8101 tgaactcaac agctaaaatg aattacggct gtgcattgga tggaatttca aatacccaaa
     8161 caatcaatcc ttcaggtctt ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg
     8221 tcttagatgg atacttctca aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg
     8281 atttattgcg tgatgcaatg gaaaatccaa tgaaatatcc gaacttgacg atccgggtat
     8341 ctggttatgc agttaaattc cgtgacttga caagagaaca acaattagat gtcttgatga
     8401 gaacttcaca cgacagaatg taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa
     8461 aggacgttga atagccatcg catcaaaatc gaataatcag tatgcttatt tgaaagaaac
     8521 gatttacagg ggcaggactt gtatgttccg tccctgtaaa ttttacacat aacgaagcgg
     8581 caattgcgaa aaattttgtt cagaaagctt cgcttcgaca gctaacttgt acttaaaatg
     8641 aaatcaaaga aaagatgtga acttaaatgg aagaaaaaac aatcggctat gttcattcta
     8701 tcgaaacttt cggttcagta gacggacctg gactgcgctt tgtcgtgttt atgcaaggat
     8761 gccgcatgcg ttgccaattt tgtcacaatc cagatacttg gaatattggc ggagggaaag
     8821 aatacacagc agatgaactt ttagataaag cagaacgctt cagaccttac tggggagata
     8881 aaggtggtat tactgttagt ggtggagaac ctctattgca gatcgatttt ttgatcgaac
     8941 tatttaaaaa agcaaaagag cggaagatgc atactacatt ggatacatgc ggaaaacctt
     9001 ttacgtatga agaacctttc ttttcacgtt ttcaagaatt gatgaagtat acagatttac
     9061 tgttatttga catcaaacac atcgataatg aagagcataa aaaactaacg cattgggaca
     9121 atgaaaatat tttagaaatg gctcaatatt tgtcaaaaat caacaaaccg gtttggattc
     9181 gccatgtact agttcctgag cgaagtgatt atgatgaata tttgatccga ctagacaact
     9241 ttattcaaac cttgtcaaat gtagacagag tagagattct tccttatcat acgatgggaa
     9301 aatacaagtg ggaaacactt ggtttgaaat atccattaga agggatcgaa ccacctacaa
     9361 aagaacgtgt cgaaaatgct aaacgattgc ttcatacaga agagtataca aaatatttaa
     9421 cgcgataggt cgcatcggaa agaagataag tattgcttct ttccgttttt ttgctattat
     9481 taaataggag aaatcacaaa ggagcgaatg acatgtcaaa aattttagtt tttggtcacc
     9541 aaaatccaga tacagatgca atcggggcag ccattgcgtt ttcacacttg caaaatgaat
     9601 taggtaaaga aacagaagca gttgctctag gtacacctaa tgaagaaaca caatacgcat
     9661 tagaccactt tcagctatct gcaccacgag tagtagagtc agctaaagca gaaaatgcag
     9721 atgtcatgct tgtggatcat aatgaattcc aacaaagtat ttcagatatc agcgaagtaa
     9781 atattttagc tgtagtagac caccatcgta ttgcaaactt tgaaacagct aatccattgt
     9841 actatcgtgc agaaccagtt ggatgtacaa gtacgattat tttaaaacta tataaagaaa
     9901 accaagtaac aattcctaaa gaaatcgcag gaatcatgct ctcagctatc atttcagata
     9961 cattgttgtt caaatcacca acatgtacgc ctgaagatga agcagctgca aaagaacttg
    10021 cagcaatcgc tgaagtcgat ctaaacactt atggacttga aatgttgaaa gcaggaacaa
    10081 atctaagtga taaatcagct gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg
    10141 gcagttccaa cgttcgtatc gctcaagtca atacagtgga ccttaatgaa gtgatggatc
    10201 gacaagcaga gctagaagca gcaatggaag atgaaatgaa agcaaatggc tacgatctgt
    10261 tcgtattagt tgtcacaaac atcttagaca gtgattcaga attacttgtt gctggtaaag
    10321 gaaaagaaaa agtagaagaa gctttccaaa caactttgac caacaaccgt gctttgctta
    10381 aaggtgtcgt ttcacgtaaa aaacaagtcg tacctcaatt gacggctgta tttagctaat
    10441 caaatggtag tatgaagcat tcaaaatgaa tggataagcg attgatataa aaagaaacat
    10501 agaacatact aaaggccgaa gaaatggagg agaataacag tcaattatga ctcttgttct
    10561 tcctcctttt tacgtaaaag ccttttaagt aaaataaaac ggatagaaaa gagaacatat
    10621 atgaactatt attcaaaaaa taaggaaaac ttttatcaaa aactgaccga tgatccgctt
    10681 ttttcttcat tgaccgatta tctatatgaa catcgagaac aagaaacgat tctaagagaa
    10741 ttgaaaaaag aattttcgca aaataaattt tcccattttc ttgatttatt gatcgatgca
    10801 ggattaatca agagggaaga acgaagatat catttgaact ttccaatatt tgatcctaaa
    10861 gattacttgc aacaagccac ttcggctgct gaaaccatag ctgaacaatt gaagagactc
    10921 tctgttgatg aacaaaaatt agcgatggga gaagttattt gggcgtattg tttcgaagat
    10981 gaacggaaag aagcgtattt ttatggagta agaaacagcc gagagacaga attgctgagg
    11041 acaactgcag gaaatgagaa gtaccgtttt atcacacttt cctctataga acacttcccg
    11101 cttactttgg ctaattactt ttttgtacag aagaatcagc ttcctgtaac gaaggcattc
    11161 aaagaattag ccgaacttat tggagacgta aatgaatcgt acttttttga tcaaatagaa
    11221 gttatcgttg acaggatacg taagaataaa tataaaaatc gccggcctag tattttccat
    11281 cagtctctac ttgtgacgaa tactataaag gaagaagaat cattcacatt agaattacct
    11341 attgtcgaaa aaaacaattt tgagattgaa ttaccaacat tggatccttc attgacaatg
    11401 gaggaaatgg cctttcttaa acgacaaatc ttttctgagc tgagcaagaa atttataccg
    11461 catgcatttt cttatatcaa agaatatgga acagttttag tgagtaaaac ctaagagagc
    11521 agtaagcaaa aattatactg actcttgcag gaggagcctt tctcttcctt tatggttaaa
    11581 agaattattt gagtattttg cctatctacc acctattaga ttcactacat ggtctggaaa
    11641 caaaggaatg caaaaaaatg gtgagaaatg tggaatgaca aaggaagtag tgataagaaa
    11701 agttagattt ttaaataatc aatattatga ttcagtcaag tatggcattt tatgggaaga
    11761 actagctgat tagattagga agtccatttg ataatccaga atcactaaga aacgaatggc
    11821 gagtcattaa taatttatcc aatcttgggt tattctttta gattgaagtg tttaaaacta
    11881 aaaaagcatt tatgaagtcg taatagtcag gcttcaaaat gctttttatt ttgtttgttt
    11941 tttctttgcc acataaatac acctagactg acgatgaaga gcatatccaa cagatttcct
    12001 gcgacttgga tcgtcgaagg gttggtcagt ggcattaaag caaggacaaa aaaggaaaac
    12061 aagctgtaaa taaaacaggt gacaggaact aatcgaaaga agagcggaca tttcaattgg
    12121 ctaaaaatga gtgaaaaaat aaaataagta aaaatggata aaatcacgac taaaccattc
    12181 atgataaaga agaaaagcgt attgtgatta aacgaataaa aaagaattgc taaaaaagaa
    12241 aacgttttaa atgcaatcaa tacgcttgaa gcttcgttta acgtaccagg atgtcgatga
    12301 aaaaaatagg caccgaaata aacagaaaac agtaagccgc aaagagaaga caaaatcaaa
    12361 aaaaacggat tttcttcggc ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat
    12421 gcactagaag gcgcatgtga ggtcttaagg tatgaataac atagaaaaaa taagatcgtc
    12481 aaagttgcaa aaaaagagcc taaatgtaaa aaattagtgg ttctacgttg aatttccata
    12541 atatctccaa attaaattga taagtatata aagtataata acaaattgat gagtatatgt
    12601 cgatgttttt taatcaaaat aataaataaa aactgtctaa catttaaaat ttaatttaga
    12661 tattagacag tttttaagtt tgtgagttat tttccttgtc gttttctcac ttttttagtc
    12721 cagaaaccac ctgaaatata ttttggctca taagaaatga taaaagcacg ctcttcgatt
    12781 tctttgatca ggcgatacag ttcacgttcg ctttttctag gagaaagtat ttcgagaact
    12841 agccgctcac cttccagccc cattgcagaa gtctgtgtca caccataacc gtgttctctc
    12901 agtatacccg gcaaactttt atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg
    12961 atgtatccta atgccaattt gtcttcaatc aaaattccga cactgatacc tactgcgtaa
    13021 cctaacgcat agacaaataa attcagtgga ttatctaaac gatctaaaac aagggataag
    13081 ccgacgatat aaatcgtgat ctcgaccata ctgacaagtg gagcaatcag tcgatagcct
    13141 ttcattgtca acatgaaacg taacgtgttc agagtgatat aagcaaaatt aatgaggaag
    13201 atcatcccca acatttttgg atcaatcatt tgtaaaactc ctttcattta tataacaaca
    13261 atatcaaaaa aaatcgtaag tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata
    13321 aaatgattat aatagaatta taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg
    13381 tgtgttttgt gccagccacg tgctaagtct ccttctcagt atatacttag gtgtctattc
    13441 gattcaacag ccaattgagg tagagaacat tgtttttcag atgtcgctga cagatggttt
    13501 aattttaagc gtttttttct ttttgatact gtttattggt aatattttcg gtagcgttgc
    13561 cagttttttg aatttttcta tttatccact cctttcgcta gcattaggag tggtgggact
    13621 ttgttctctg tgcttatttc ccgagccatt cagtcctgca tttatattat ttggtacact
    13681 gaacctgttc cagacaactg ttggagcatg gttgctatgg cgatcaggaa atttaatgaa
    13741 aataggtgag taacaattga atccaattat attgaaacgt tctgtccatc atattccagt
    13801 gttagaagtt gttctgaaag aattaagaga ggaacgtatt cccgttgtca tctattatca
    13861 cggatggcag acaagtaagg agttagtttt gacgcaagga agaaaaattg cgcagcaggg
    13921 cattcgtgtg atcctgccag atgcgatgaa tcatggggag cgcaaacagc cggtttcttc
    13981 tattccatcc tatacatttt gggatagtat ctacggaaat ctgtttgaat ttgacacact
    14041 tatcaagcat ttagaaaaaa gaggtcttct acatgatcag cttgcagttg gcggggtttc
    14101 gatgggggga atgacaacat gcgccttgtt ggctaaacat cctcatatag tagcgggcgt
    14161 gtgtttgatg ggttcccctg cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc
    14221 ggaatataat tatgtcgtac caaaagacta ttttgatttg atttcatggg ttaagagcta
    14281 tgatttgtca ttgcagccgg aaaagttagc tggacgtcct ctgttctttt ggcatggaca
    14341 agctgacgaa aaaatccctt atatacaaac ggaacgattt gtagaaaaaa atcggcataa
    14401 gtcttacgga aaagaaattg tttttcgttc ctcaaaaaca cagcgacata tggtacaaat
    14461 accactaatg gaagaagctt ccactttttt agcaaaaaat ttgataaaaa agtataaata
    14521 attgaaacgg aaaatcactc atgacgatag aggattttcc gttttttatt ttgggctata
    14581 ataaactctg ttaggaaaat ttggtataat tgatagtaag aatgacaaca tgttagaaag
    14641 gaagagagca tgcccacttt agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg
    14701 gtctctcgag tcatcaatca tccggaacag gtgactgacg aattgaaaga gttagttttc
    14761 tcagcaatgg ctgaactgga ttaccgtcca aatattgctg caaaagcact tgtcacgaat
    14821 cggtcgcaga tcatcaaatt atttatactg gaagaaatcg acacaactga gccctattac
    14881 atgaatttgc tgatgggaat cgctaaacgg atcggtaaag cgcattattc tctccaatta
    14941 gttacaaatg atgccttcga cgtcggttct tgtgatggat acatcatcac aggtgtcaga
    15001 aaaaaagact tcgattggat cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat
    15061 tctgaaggat atgattatgt agacagcgat aatgaatatg gtacagcaaa agcaacgcaa
    15121 tatgccttag aaagagatta tcaaacgatt atttatatcg gtatcgaagt ggacgagcct
    15181 tttgaaattt caagggaaac tggttattta tctgtgatga aggaagttga tcttcctagt
    15241 aaaatcttgc ggtttccaaa tcattcgaca gaagtagaaa gatatatgac agaaaactgg
    15301 gcagatttta cagataaaac ttgttttgta tgtagttcag atcggctagc actaggagca
    15361 gtaagaggga ttataaatgc tggaggacac ttgcccgaag aatttggtgt gattggattt
    15421 gatggggtat ttttagacca aatcagttca cctaaattga caacgatcaa gcaagatatt
    15481 atacgtatgg gtgaagtatg tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg
    15541 agtcaaggtt atcgtcgttt ttttcctgaa ctagttatca gagagacaac gaattaagtc
    15601 ttattactat atatatattt atgaaattca tcgctatttt cttaaaataa tcagcgcatt
    15661 gctactgaaa acaatgaatc tgtgctatta ttgagaaaag ttccattttt gagaaggatg
    15721 gtttgaatga aagaaatcag acgaaaacat ttccgttttc ctgcgtacga tgatgagtta
    15781 ggcgtaaaat taaccaacga caagaagcga gtattatttg acggacaaga agatcttttg
    15841 actgaacaga atcagtcccc gtttgaacag atgaaagaat ttcatttcac gaacacgaac
    15901 accgagaaaa tccgtcgcaa gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag
    15961 gaaaatttgg aaaagcataa aggaaaatta ccggattata ccggccatta ccaatcagaa
    16021 atcacatcga caggacgaaa aaaacttttt ggtgattcct ctcgttctgt cagacaaagt
    16081 gaaacaagga cagagtccaa gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg
    16141 gagcgttctt attttgttcc taaatatgta cccgcatcga ttatcccaga ccctagagaa
    16201 agcaaaattt ctgaggatca tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg
    16261 ttgttcgata cagaaccgac gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga
    16321 acattcaatc gaacaaaaac gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa
    16381 aaaagccatg gtatcttaga acgctcatta caaggattga tcgatgatca aggaaactct
    16441 ttgggagaaa atagctattt taaataagtg atatacaaat ttatattcgg ctacagttgg
    16501 aaaggaaacg ctgcggcgtt tccttatttt atactctatc cgaaagttta tatcgtatcc
    16561 tcaaattttt ttgagtttaa gcgaattttt tctcccaata agtaagaaaa taatggcaaa
    16621 ataaaaaaac aaactatttt cgcagatcgt ttgcaaaaat agtttatttt tttataaaga
    16681 agattttact gaataatatc attttagttt acataatgat atttctgtaa attaaataaa
    16741 gtagtgaaaa ataagaatca gtgctctaac tgttcaagag gatcaagtgg ttcaaatgtt
    16801 tctgtagtct gttttgcttc caattggggc aaaacagatc caatttccag atacttcact
    16861 ttattcaatg tataagatag aatagaacag ctaagtaatg tatacagaaa atgtgtgaga
    16921 tccaaataaa gcaaggagac aactaaaata ccaaaagtaa tcaagaaaag acttttgcca
    16981 atcttaatat gaaaatattt ttcaatggct tgataaatga tatctgttcc gcctgtgctt
    17041 ccgccgaatc gaaagacgag accgcaacca atgccagaaa gaatacctgt catcaaacta
    17101 ttgatcagta gactatctac agcaaaatgt atatgaaggc tctcccataa atgtaaaaag
    17161 aaaactagcg caaatgttcc gacgatcgtc cggagaaaag cgtgttttcc aaaaagaaac
    17221 aatgagataa gcaataaagg taaattaaaa agcagtgaag tgacagaagg agcgaatcca
    17281 aaaagattat gcaaaagcaa agcaatacca gtaaccccac catctgccag cttagatgga
    17341 atattgattc ctgtcacagc taatccgtag attcctgcgc ctaaaatgat tccgcacaat
    17401 tctgtaaata aatttaattt tttcatttct agcctctttt cttttgaaaa ttttcagcaa
    17461 cttctttatt atactgactt atactcttac ttcaagagga aaatgaaaaa aaattatttt
    17521 tggctgtttc tttttattta tcaaataagg agtggaaaaa tgcgagcagc agacttattg
    17581 acaattctta caaaatcacc caaaaaaaca gagtatcaag gagttttttt ggctcaagaa
    17641 caaaaactgg tgcctgtgac aatggtccag ataaataaag caaatcaatt tatcttttta
    17701 tttgaacacg gcaaagcacc acttgctatg aaagacatct ttcttttttt gatgaagaac
    17761 cgtgagaaag ctgtttatta tcagtcaaaa gaagaactca ttcctcttta tggagtgaaa
    17821 gagatagaaa ataaagtggt gatataagta aaaataagaa aacaagacgt ttatgatgtc
    17881 aaaacaacca ttttttacat ttttggcaaa aaagtgtcat gatacagtat aatattcgtt
    17941 gaacaggcca ataaaggtgt tctcccccac gagatttcta ctttattggc tgtttctttt
    18001 tattaaaata aaagttgtgt gtgtaaaaaa gatatagctg attccaagtg acaatttcgt
    18061 atagattttt atggtattcg tagtgaaaaa acgagaacca aaaagttgat ccgactttgt
    18121 tgcttggatt ctttttttgt cagcatattt agaggtattt ttttcttttt tttgttcgtg
    18181 ctgtaaggtt aaggcaagac aagttagttg ggaaaaagga aagaaatgtt tatacttaaa
    18241 gaataacaga aaggaaggtc gcatgaataa ttttatacaa acattaagtg aaagaaaagg
    18301 cgagttgctg gaagctgcat ttcaacatct gtccatttct ctcatttctt tgctgattgc
    18361 agcactgatt gctattcctt tagcgatttg ggcagccaat cataaaaaaa tggcagaagt
    18421 cttattgcag atcacgggtg tgctccaaac gattccttca cttgccttat taggtttact
    18481 tatcccattt gtagggatcg gaactgttcc agcattgatt gcacttgtta tttacgcttt
    18541 gcttccgatt ttccaaaaca cgtatattgg tctagctgaa attgacccgt ctattgaaga
    18601 agcagcggta gcatttggta tgtccagaat gagacgcttg ctgaaagtag agcttcccat
    18661 cgcattacct gtcattatct caggtatccg aactgcctta gttttaatca tcggtacggc
    18721 tactttggca gcattgatcg gagcaggtgg attagggaca tttattttgt taggtattga
    18781 ccgaaataca cctgttttga cattgattgg tgcgatcagt tctgccttgc ttgcaattat
    18841 attcagcgga ctgattcgct ggttgcagca taagaaacct cgatattcca tcattacttt
    18901 agcagcagtg gtgtgtttat ttggcggcgt ttctttatat cagtctgcct tgtttaaaaa
    18961 tgaaaccatc acgattgctg gaaaattagg agcggaacct gaaatcctga tccaaatgta
    19021 taaagaattg attgaagaca acacaaaaac agaagttgaa ttaaaaccga attttggcaa
    19081 gacgagtttt ttgttcaatg cactggatca aggtcaaatc gatatctatc ctgagttcac
    19141 aggtactgtc ctagaaagct tggtgaaggt acctgaagca atgaaaagtg ctgatttgac
    19201 aaaagaaaaa acatatgacg aagcagaaga attattgaat aagcaattcg atatgacatt
    19261 attaaaacca atggcttacc aaaatacgta tgccttggca gtcaagagat cttttgcgaa
    19321 cgaacatcaa cttaaaaaga tttccgattt ggcagctatc tctgatcaga tcaaagcggg
    19381 attcacactg gaatttattg atcgttcaga tggatataaa ggaatccaag aagcatatgg
    19441 cttgaatttt tcaagtgtac aaagcatgga gccaagtttg cgttaccaag caatcaataa
    19501 tggagatgtc aatattgtgg atgcctattc cacagatagc cagctcaagg agtacgatct
    19561 agtgactcta gaagatgatc gagggttatt cccagcttat caaggcgcac cgctgatgaa
    19621 aaaagatttt gctaaagaac atcctgaaat cgttaaagca ctgaataaat taagcggaaa
    19681 aataacagag gatcaaatga ttgatatgaa ttaccaagtc aatgtcgaaa agaaaaagcc
    19741 gacagaagta gcacgaatct tcctgaaaaa tgaaggatta gtaaaggagg aagatcagtg
    19801 actaaattaa tcgagttcaa acatgtgcaa aaaaagtatg atggaaagta tgtcatcgat
    19861 gacctaaatc tggcaatcaa aaaaggagaa atctttgttt tggttggtcc ttccggtagt
    19921 ggaaaaacaa ctactttgaa aatgatcaat ggattatcta aaccatcagc aggagacatc
    19981 tacttcaaag gaaaatcgtt aaatgaatat aatctgcaga aaatgcggtg gaatatggga
    20041 tatgtgctgc agcaaatcgc tttgttcccg accatgacag tcaagcaaaa tattgaagtc
    20101 atccctgaaa tgttaggttg ggaaaagcag aaaagagcag accgtgtaga tgaacttctt
    20161 caaaaagttg gcttatctcc tgatatttat cgagatcgta tgcctagaga gttgtctgga
    20221 ggagaacagc aacggatcgg gatcatccga gctattgctg caagcccaga tgtgattttg
    20281 atggacgagc catttagcgc attagatccg atttcaagaa actcattaca agaattggtt
    20341 ttgtccttac acgaagaatt gggcacgacc atcgttttcg ttacgcacaa tatggaagaa
    20401 gcaatcaagc taggtgatcg tattgcattt atgaaggatg gagaaattat ccaatgtgac
    20461 acaccagagc agttattgat gaatcctaaa aacgactacg ttcggcattt ctttgatgaa
    20521 ccgaaacaga cgaaagagtg gcgagtagaa gatttagtcg tcaatggata tttcttaaat
    20581 gaaataccag aaaatgctcg tcaacaagtt tgctttgata cgcctatgaa agaagtatat
    20641 tcgcttcttt cggtgtatcc aagcattgtg atagtagaaa agcaaagaag tattgggtcg
    20701 ctgacaagta aagaaatctt tgctttttta agtcgagagg aggaaggaaa tgaaaacata
    20761 tgatgcaatc atcatcggtt caggtgtgag cggcttaagt gctgcttatg gattgaaaga
    20821 agcaggtaaa acagtactag tggtcgaaga agatctatgg ggcgggactt gtcctaaccg
    20881 tggatgtgat cctaaaaaag tcttgttaag tgcagtagag gcacgtaatc gggtaaaaca
    20941 attgagtgga aaaggtttta acgagatccc aactgccaac tgggaagaac ttcaaaaatt
    21001 caaacgaaca tttacggatc cagtcccaga aagccgaaaa aaacaattag cagaagctga
    21061 aatcgatcat ctttcaggaa ccgctcgttt tttggatgat tcatctattg aagtcaacga
    21121 agaagtcttc catgcggatt acttagtttt ggcaaccggg caaagaccta cgatcttacc
    21181 agtggaggga aaggaatact tgaaaactag tgcagacttt ttatcattgc ctgtactgcc
    21241 taaagaaatc atttttatcg gtggtggtta tattgctttt gagctagcta cgattgccaa
    21301 tgctgcaggg agcaaagtaa ccattgtcca tcataatcaa cggccattaa aagaatttga
    21361 ggcgtctttg gtagaggaag ccgtccatca aatggaagca tctggcattc agtttgcttt
    21421 tggtgttgaa acccaaaaga tcatttcaga aggtactcgc tatcgtttag tcggaaaaga
    21481 gacggaatta gtagcagata tgattttttg tgcgactggg agacagccga atacagaatc
    21541 tttagcactt gagcaagcga atatcgtctt tgataaacac ggaattgcag tcaatgatta
    21601 tctgcaaacg agcaatccaa aaatttttgc gtgtggagat attgtttcaa ggaaaacccc
    21661 aaaattgaca ccagtcgcca cttttgaagg aaattatgta gctaagcgga taacggatgc
    21721 aacaagcgaa cctatcaaat atccgattat acctacgatc gtctacgcca gtcctaaact
    21781 tgctgaagta ggagttacaa aaagtcacgc ctcttcgtca gatcaagtgg tagagatgga
    21841 tcttaccagt tggttcacct atcatcgtgt caatgaacca gttgctaagg ctgaattgac
    21901 ctttgaccag caaaattatt tgattggtgc tgcagtgatt agcgagcaag cagacgaatt
    21961 gatcgatgat ttgacactcg tcatcaatca aaaattgact aaaaaagagt tggatagcta
    22021 tatcatgggg tatccgacat tggcaagtga tctctcctat ttattgaaat aatcagcatg
    22081 ataactaaag cgcttgttgc tttttccttc ttgtgctaca ctttaaaaaa tgaatacctg
    22141 tttctgggga tgtcgtagac gacatgagag taagttgtga acttagaccc attgaacctg
    22201 tcagttaata ctggtgaagg gagaaacata gcagacgctt gaagcgggtt actatgtcgt
    22261 gcccatttca agcgtttttt gttattcata ttagaagaaa ggaggtaaca aaatgaagaa
    22321 aaatctttat ccagcagtca gtatggcatt cattttaatt atttggcaga ttgttgtcat
    22381 agtttggaaa atacctgcct ttattttacc cggacctgct gctgtatttt ctgcattagc
    22441 ggcagatgca caagtattac tttcccattc tgtcacgaca ttaaatgaag cagtgcttgg
    22501 tttggtaata gctagcgttc tttcttttat tactgccttg gcaatggatt attggaagtt
    22561 tttagaaatg tctttttatc cgttactagt gatttcacaa atgttgccaa tcatggtgtt
    22621 agggccatta cttaccctat ggtttggttt cggtatgctt ccaaaagtga tccttgttgt
    22681 cttgataagc tattttccga tcgtagtttc tttttcggat acattgagga aagtatcaaa
    22741 agagcaaatc atctttttga aaacgatggg ggcagataca gggaagattt atcgaatata
    22801 taaaatacca gcgggtttat ccggcttctt ttccggttta aaagtggctg ctacttattg
    22861 tgtcagcgga gcagtcgttg gagaatggct aagcgctcaa gcaggcttag ggtattacat
    22921 gatacgcgta aaaaacagct accagatcga taaagttttt gctgcgatca tttgtgtcat
    22981 tctattgagt ttattgctga atggcgcttg ttcattattg aaaaaagggt attatcgtat
    23041 gctttatatt ttttaagaga aggggatcaa atgaaaaaat tatggctttt attaccgcta
    23101 ctgttgcttt tatccgcttg tggcactgca aaagaaacaa gtagcaaaca agaaataaag
    23161 gatttgaaaa aagtgacact tgttttggat tatgtaccga atactaatca tactggcatc
    23221 tacctagcaa aagaaaaagg ctattataaa gaggctggtt taaatgtcca aatcatcgaa
    23281 ccaggagata attcgacaag tattggtcta gtaggtgcgg acaaagcaca atttggtgta
    23341 agttatcaag aagatgtcac ttatgctcac gcagatggac agaatatacc agtcaaagca
    23401 attgctacag tgatcaaaca taatacttct ggattcgcca ccctatcaga cagtaacatc
    23461 cattcaccaa aagattttga aggaaaaaca tatgcaggtt ggcaaagtcc aagtgaagaa
    23521 gctgtcttaa aagctgtgat ggaaaaagac ggcggggact tttcaaaact gacaatggta
    23581 ggaagcaatg gagaagggcc agaaagctta ggaaaatcct cagatatcca atggtacttt
    23641 gaaggttggg atatgatcaa agcaaaagaa gcgggtattg aactaaacta tatcccattg
    23701 aaagaattag atgaacggtt agattactat actccggtaa ttattacgaa tgatcaactg
    23761 atcaaaagtg atccagaatt ggtacagtct tttatggatg ccacgaaaaa aggctatcag
    23821 gaagctatta aggatccaaa tgacagtgct aaactgttgc aaaaatatgc aaaagaaaat
    23881 gatcggacgt ttttagaaga atcacaagca ttcttgtcaa aaaattatac ggatgatcct
    23941 gagaattggg gattgatgga agaaaaagtt tggcgcaatt atacagcttt tatgcaggaa
    24001 aacggattga tcaagcaaga tgtaccttcg caagaactat tcactaatca gtttataaaa
    24061 taggagtgat agatatgtca atccaaatca gccacttgaa tgtagtcatt gaaaacctga
    24121 aaatattaga agacatccat ttcactatcc ctaaagatac ctttacatcg attgtcgcgc
    24181 caagcggagc cggtaagtca acattgctga aaactttaac aggagtccag cctatgactt
    24241 ctggaacgat caaagtagac gatcaaaaag taactggtct ccatacagca tttagttata
    24301 tgccgcaaga agatatgtta cttccttggc tgagcgtcta tcaaaacgtt acgttatatc
    24361 aaaaaatcaa tcatctaaca atcaatgaag aacaagtaag ggattatctg gatatctttg
    24421 ggttaaacgg ctatgaacat tttttgccgg aacaattatc tggcgggatg aagcagcgaa
    24481 cggccttgtt acgaacaatc atgaatcctt cttcttactt gcttcttgat gaaccatttg
    24541 gagcgttaga tgctatgacc agagggcaga tgcaagactg gatgttgaag ctgcctaaaa
    24601 aagcaaagcg gactacttta cttgtgacgc atgatatcga agaagcaatt tatttatcag
    24661 acaggattct tgtgctttcc gcccgtccgg cacatgtgat tgctgagatt caagtgcctg
    24721 aaaaacgacg gtcacgtgaa tggctactgc agcaatcaga gttgaagcaa acgatttatc
    24781 agttattggc aggtgaatta gatgttaagt gatgctcatt gtcatttaga tgggagtcag
    24841 agtttagcac tccttcagca ggaacacacg attttgacga tcatcaattg tgattcgcca
    24901 gaagaatgga aggaaaacag acagcttgca gcaagtaaga ctcaagcctt aagttacggt
    24961 atccatcctt ggaaagcaga tagctatacg tttgaacaag ttgagccttt tttgaaaaaa
    25021 gcaaggatca tcggtgaaat agggttagat aatatttgga cgaatgtacc gatgactaca
    25081 caaaaaaaag tatttgagcg acagcttgct tttgcagcca taaatgagaa acctgttgta
    25141 ctgcacacaa aaggatgcga aaaagagatc ttaaattata tccaagctta ccctaatcac
    25201 tatttgatcc attggtattc ttctttggac tatcaacaag actatattga tttaggctgt
    25261 tatttcagca ttggtcttga tttgaaaaag aatccagcag tctggcaact agctaaagca
    25321 gtcccaatcg accacttact tattgaaacg gatggactac atgcagcaga gtgggcttta
    25381 ggggggattt tttcagaaaa agattatgtc tcactgcttg aagaacagtt aaagacaatc
    25441 gctgagatca aagagctatc agttgaagaa gttaaaaaaa atacttatca aaacttggaa
    25501 cgtttcatta gattaggtaa ataaaaaaga gatgtgacac aactagtgtc gcacctcttt
    25561 ttggctttct ttgtataaaa gtaaactgtt tgattaataa atgaatctta gtaccaaccg
    25621 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    25681 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    25741 tggtaacgtc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    25801 gcgatccatt cttttgcaga gcttgtgctt gctggagtag cagcagtttc tgttacagca
    25861 ggagcaggtt gtgtttcagc aacaggcgct gcttgttttt ccgtttgaac cgtttcttgt
    25921 acaacaggtt gttcagcttg gactggtgct tcttgaacgg ctgcttgagg ttcagcagct
    25981 gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DACCDF010000035        25810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCDF010000035 DACCDF010000000
VERSION     DACCDF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.035"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            9..851
                     /locus_tag="IVK51_001456"
     CDS             9..851
                     /locus_tag="IVK51_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP6431058.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            848..1798
                     /locus_tag="IVK51_001457"
     CDS             848..1798
                     /locus_tag="IVK51_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP6431059.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            1813..2046
                     /locus_tag="IVK51_001458"
     CDS             1813..2046
                     /locus_tag="IVK51_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP6431060.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            2377..2961
                     /locus_tag="IVK51_001459"
     CDS             2377..2961
                     /locus_tag="IVK51_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6431061.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            3092..3760
                     /locus_tag="IVK51_001460"
     CDS             3092..3760
                     /locus_tag="IVK51_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP6431062.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            3762..5186
                     /locus_tag="IVK51_001461"
     CDS             3762..5186
                     /locus_tag="IVK51_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6431063.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            5204..5773
                     /locus_tag="IVK51_001462"
     CDS             5204..5773
                     /locus_tag="IVK51_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP6431064.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRFRQDWIDRGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            5883..7637
                     /locus_tag="IVK51_001463"
     CDS             5883..7637
                     /locus_tag="IVK51_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431065.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            7591..9363
                     /locus_tag="IVK51_001464"
     CDS             7591..9363
                     /locus_tag="IVK51_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431066.1"
                     /translation="MRMQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            9886..10155
                     /gene="rpsN"
                     /locus_tag="IVK51_001465"
     CDS             9886..10155
                     /gene="rpsN"
                     /locus_tag="IVK51_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6431067.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            10170..10349
                     /gene="rpmF"
                     /locus_tag="IVK51_001466"
     CDS             10170..10349
                     /gene="rpmF"
                     /locus_tag="IVK51_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6431068.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            10381..10758
                     /locus_tag="IVK51_001467"
     CDS             10381..10758
                     /locus_tag="IVK51_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431069.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            10779..10928
                     /gene="rpmG"
                     /locus_tag="IVK51_001468"
     CDS             10779..10928
                     /gene="rpmG"
                     /locus_tag="IVK51_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6431070.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            10952..11935
                     /locus_tag="IVK51_001469"
     CDS             10952..11935
                     /locus_tag="IVK51_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6431071.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            11965..12087
                     /locus_tag="IVK51_001470"
     CDS             11965..12087
                     /locus_tag="IVK51_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP6431072.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            12105..13628
                     /locus_tag="IVK51_001471"
     CDS             12105..13628
                     /locus_tag="IVK51_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP6431073.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            13652..13891
                     /locus_tag="IVK51_001472"
     CDS             13652..13891
                     /locus_tag="IVK51_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431074.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(14084..14545)
                     /locus_tag="IVK51_001473"
     CDS             complement(14084..14545)
                     /locus_tag="IVK51_001473"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6431075.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(14740..14958)
                     /locus_tag="IVK51_001474"
     CDS             complement(14740..14958)
                     /locus_tag="IVK51_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6431076.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            15076..16692
                     /locus_tag="IVK51_001475"
     CDS             15076..16692
                     /locus_tag="IVK51_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP6431077.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(16933..18273)
                     /locus_tag="IVK51_001476"
                     /pseudo
     CDS             complement(16933..18273)
                     /locus_tag="IVK51_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(18549..18704)
                     /locus_tag="IVK51_001477"
     CDS             complement(18549..18704)
                     /locus_tag="IVK51_001477"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6431078.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(18896..19090)
                     /locus_tag="IVK51_001478"
     CDS             complement(18896..19090)
                     /locus_tag="IVK51_001478"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6431079.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(19000..19332)
                     /locus_tag="IVK51_001479"
     CDS             complement(19000..19332)
                     /locus_tag="IVK51_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6431080.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(19846..20037)
                     /locus_tag="IVK51_001480"
     CDS             complement(19846..20037)
                     /locus_tag="IVK51_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6431081.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(20131..20517)
                     /locus_tag="IVK51_001481"
     CDS             complement(20131..20517)
                     /locus_tag="IVK51_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6431082.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            21211..22416
                     /locus_tag="IVK51_001482"
     CDS             21211..22416
                     /locus_tag="IVK51_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP6431083.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            22433..22867
                     /locus_tag="IVK51_001483"
     CDS             22433..22867
                     /locus_tag="IVK51_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431084.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            23484..>25810
                     /gene="esp"
                     /locus_tag="IVK51_001484"
     CDS             23484..>25810
                     /gene="esp"
                     /locus_tag="IVK51_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP6431085.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         8896 a         3700 c         4829 g         8385 t
ORIGIN      
        1 ggaataatat gcttcaaaat tttattaatg gtctgtatga ctttcacttt ttacagaatg
       61 ctctaattac atctgtagtt atagggattg tttctggaac agtaggttgt tttattattt
      121 taagaggaat gtctcttatg ggtgatgcta tttctcatgc tgttttgcca ggagtagcaa
      181 tttcttatat tttaggtatt aattttttta ttggtgcgat tgtatttggt ctattagctt
      241 ctatcattat tacttttata aaaagcaata gtgtaataaa aggagataca gcaattggta
      301 ttactttcag ttctttttta gcattaggag ttatccttat aggagttgca aatagctcaa
      361 cggatttatt tcatatctta tttggaaata ttttagctgt acaagatatt gataagtgga
      421 ttacaattgg cgtttctgtt ttagtgttgg ttacagttgt tctatttttt aaagaattat
      481 taatcacttc ttttgatcca ttaatggcaa aagcaatggg gatgaatgtc agtttttatc
      541 attatctatt aatgattcta ttaactttag tagcagtaac agctatgcaa agtgtgggaa
      601 ctatattaat tgttgcaatg ttaattacac cagctgctac agcatattta tatgcaaaca
      661 gcttaaaaac tatgatattt atttcggcga cagtcggagc gtgctcatca ttgctaggtt
      721 tgttcattgg ttatagtttt aacatagcag caggatcaag tattgtttta acagcggcat
      781 tcttatttgt aattagtttt ctaatctcac caaaacaaaa atttttaaga aagaaggaga
      841 gaaagttatg atgagaaaat ggaaagcagt actgggaagt ctggggattt tgattgctct
      901 ttttatattc ggggcatgtt caacaaatag taaagacaaa gatacagtgg cttcgaacga
      961 aaaattaaag gtagtagtta ctaattcgat tttagcagat attactgaaa atatagcaaa
     1021 agataaaatt gatttacaca gtatcgtacc tattgggaaa gatccccacg aatatgaacc
     1081 tttgcctgaa gatgttcaaa aaacttcaaa agcagatttg attttttata acggtgttaa
     1141 cttggaaact ggaggaaatg cttggtttac aaaattagta aaaaatgcga acaaagagga
     1201 aaacaaagac tattttgcag caagtgatgg catagatgtt atttacttag agggtcagag
     1261 tgagaaaggg aaggaagatc cccatgcttg gttaaattta gaaaacggta ttatttacgc
     1321 taaaaatatt gaaaaacagt tagcggaaaa agatcctgat aataaaaaat tctataaaga
     1381 aaatctagat aagtatattg aaaaattgga ttctctagac aaagaagcta aatctaaatt
     1441 tgcttcaatt ccgaatgata aaaaaatgat tgttacaagt gaaggatgct ttaaatattt
     1501 ctcgaaagcg tataatgtgc cttctgctta catttgggaa atcaacactg aagaagaagg
     1561 aacaccagat caaataaaac acttagttga aaaattacgc acaacaaaag ttccctcctt
     1621 attcgtagaa agcagtgtgg acgatagacc aatgaaaaca gtatcaaaag ataccaatat
     1681 tcctatctat tcaacgattt ttactgattc aattgcagaa aaaggacaag atggtgatag
     1741 ttactatgcg atgatgaaat ggaacctgga taaaattgct gaaggccttt cgaaataatt
     1801 tggagggtag gtatggtaca aattaaaacg actactatac taatttattt actagcaatt
     1861 caggaattat caaaaagaaa aaagaaatta aaaaataatg atttagcaaa agttctaagt
     1921 gttagtccag catctgtaag tgagatgctg gctaaactta gatatgagga ctatttagat
     1981 ataggatttc aattaactga taaagggaaa aaatttattg acgattacaa aaaaggtttt
     2041 aagtaaaaga ctttattttt agggaggata agaaaatggc tattggagtg attatgatag
     2101 gacttaccct aatagctgga tataattttt ttaaatcttt ctcggattat tttagaaaag
     2161 gtgaatgatt tagatgagtc tattaattct tgtaggattg ctttttgtat cagttatttt
     2221 gctctttctc attagtttag ctctaaggaa tgagttcagt aatagacgtt aacgattgaa
     2281 acttcaagtc ctgtgtaaga taatatagtt aattcatctt attaggagga agtacccata
     2341 gaaaccagag attatattac aactcaccaa aaattaatag aaagtggact aagccatttt
     2401 gaaaaagaag gatttcagca aactaagata aggcaactgt gcttgaccgc aggcgtaact
     2461 acgggtgctt tttataaaca ttttacttct aaggaagcgt tatttgaaga aattattcag
     2521 ccgcatatta atgcgcttat taatatgtat aatgaagagt ttaccaaata cctcttaaac
     2581 ttagaaaata tagatataga gaaaagttgg agagagaact tcaatgaatt agatccattt
     2641 attaattata tttttgagaa taaacgtata ttcgatttgg ttttattcca atcagctgga
     2701 accaaatatc aagatattgt tgaaaaaatt acatcatttg tgacaaaaaa gacagtctgt
     2761 tgtctagata agttaaagac tatcggaatg atcaatcaaa atttagtctt taatgaagaa
     2821 gaaatccact tctacattta ctcattctac gctacattct atgatatatt gaagcatagc
     2881 tattcaaaag aaaagactat gctattaacc agaagattgt atcaattttc tgttcctggg
     2941 tggattgaat tactgagtta attaaaaaaa agcttgcatt gagaattgtt ctcagttatg
     3001 ctttttgata agatcgcact gttatctgaa taacggtgca tttttgtcag cttaagataa
     3061 cagtgcgatc ttattttttg gaggtgaaag agtgtcaaaa atcaatccgt taaccttgat
     3121 tactttaaat attttttttc cggtcattat ttttttagga aagggaaata tatttcaagt
     3181 ggggtgtttc attattgcta cagttttagt attgatgttt cgtaaaataa agacatgtat
     3241 ttatttcatc ttgatttatt cagttctgtt tttagtaatg aaaattattg atgctatcaa
     3301 gatagagttt tttactatat tttttgggac atccttatat attgtatttc gaatgttgcc
     3361 ggtcgtaatg atctcatggt tgttggttag tgtctatact agcaatgaat tattatcctc
     3421 tttagaaaaa atacatttac cgaaaaaaat aatgttaagt attacagtag tgatcagatt
     3481 ctttccgact tatcgttctg aaataagaat gattaaagaa tctttaaaaa tgagaaacat
     3541 tcgcttgtct ttttttcagc ctctgcaata tttggaattt ctgttagttc ccgttcttat
     3601 acgtgcaact atgattgctg aagaaatgac cgctaatgca ataacaaagg ggatagaatc
     3661 tcctgtgaaa agaagttctt actataaaac aaacatgagc ttattagact atatcatcct
     3721 gttttccgtt actatttttt tcttaagtat tttgatttga ggtgaaagaa ttgtttgaat
     3781 ttaacaatgt ttctttttca tataaggatg ttaaggaaca agcgcttgtt gaccttaatt
     3841 tttcaataaa aaaaggcagt cttacattga ttactggttc ttctgggagt gggaaatcca
     3901 cactattaaa gttaatgaat ggacttattc caagacttta tgacggaaaa ttggttgggg
     3961 aaattttatt tgaaaataac tcgctacaat ccttaacaca agaaagtata tcaaaaaata
     4021 ttggctacgt aagtcaaaat cctagaggac aattctttac aacaaataca acgagtgagt
     4081 tagtattttc gatggaaaac tttggattta caaaagagca tatggataaa agaatcaatg
     4141 aattagctga attattagat ctttcaaata tattagataa aaacattttt tcgctatcaa
     4201 gtggtgaacg gcagaaaatt tctattggat gtagtttatc gctaaactct aaattaatta
     4261 tattggatga accgtcttca aatttggact tcaaagcgac taagaaactt gctgacttac
     4321 tttgtgattt aaaaaataaa ggatttacta ttgttatagc tgaacatcgt ttttactatt
     4381 tgaaagaatt aattgatcaa gttctatttg ttaaaaatag aactgtaaaa actacaacta
     4441 ttcagttgtt aaaaactgaa aacaataacg cattaaggaa atttgacatt tttaatagtt
     4501 cgattgaagt tccctatatc gaaaaggtaa gtgcagatgt attaaaagtt gaagaaatta
     4561 cttatgaaaa tattctaaaa gacataaatt ttgatgtcaa agttggagat gttattggtt
     4621 tggtagggaa aaacggtgtg gggaaaacaa ccttactaag aatactcatg aagattattc
     4681 gccctaccaa aggaaagata atcgaaaata aaaaaaattt gtcatcgccg tttttagtaa
     4741 tgcaagaaat ggattaccaa tttttcacag aatcagtgag gagcgagtta gccctgggga
     4801 atgaaatagt tagtaaagat cagcaagaaa aaattttaaa aaaaatggaa ctttataaaa
     4861 tgaaagatca attaccgttt gatttatccg gtggagaaaa acaaagatta ttggtttcta
     4921 tagcttcatt gtcaaaaact aatttatttc tatttgatga acctacaagt gggttggatt
     4981 atatcaatat ggaaaggatt gctgacctta ttaaagactt aaaagaaaag ggtgcagtag
     5041 ttatagccac tcatgatccg gaatttcttt ataaaacatg caatagaatt atttatttaa
     5101 aaaatggtgg aataaaaaaa gatataaaac ttagagtaga agattcaact ttgatagaac
     5161 agatttttaa tgacataacg aaatagaatt gaggagaata tatatgaaaa caaaagatta
     5221 tatttttatt ggtttagcta cagttatttc cctagtaatt tacttttttt caataatggt
     5281 tagtagtatt ggtggggcgt ttggtcacag catatcccca gggatttttg gattattatc
     5341 tggaatcata tttgtttata ttagctataa ctaccctaga aaaggaattt ttactattta
     5401 cacaattgtt ttattgttat tttttacgtt aatgggcggg gcgtacttac cttggtttat
     5461 cagttcgatt agtatggcga tcttagctga tattattcta tctgtatttg gatatgatcg
     5521 agcaattcct caagttgtgt cttgggttct aatgcaacta ggcagtgctg ccggacagtg
     5581 gattcccata tggttcttta cagatcgttt tcgtcaagac tggatagata gaggacaatc
     5641 tgctgcaaca atggatgcaa tgatccacta cgcggtgggt atatggggaa ttatttcagt
     5701 gttagttgta gcaagtttat ctatgattgg tgtattgatt ggaagaaaag tcttaaaaaa
     5761 atacaaaaaa tagaacgtag tttaccttct atctgtttgt acatctctaa tttacgctat
     5821 tacggaaagc attaacagtt gtaaacgaat aagagaagaa attgaatgcg ggggaggaaa
     5881 aaatgaaagt atacaaaaaa ttatttagtt atgtccctaa aatgaaatac tacggatggt
     5941 tagccacttt attttctgat ttttctgtcg tattaacagt ctatggatat tacagtattt
     6001 acaaatttct tcagtctcta attattgtag gagatgaata tttagctaag agttatgcgg
     6061 ttcaaactgt tgcttggtta actttaggag ctttatttta tattttttct ggcttatttt
     6121 ctcatatcct tgggtttcgc ttagagacta atttgagaaa aaagggaatt agcggtttaa
     6181 cagaagcaag ttttcgtttt tttgatttaa attcttcggg atatattcga aagacaattg
     6241 atgacaacgc ggctaaaaca catatggcaa ttgctcactt gattccagat agtgctcaag
     6301 ccatagtaac acctgtttta tcagttgcct taggcttttt cattaacttt aaaattggat
     6361 tggttctaat cgccatgtta gtaattggtg tattcttgtt aaagaaaatg acagagaata
     6421 caaattttat tcaaaaatat caagaatcgt tagatgtgtt aagctctgaa acggtagaat
     6481 atatccgcgg aattcaagtt ataaagatat ttggtgctaa agtaacatca tttaaagcct
     6541 tgaataaagc aattaacgat tatgctaagt ttgcatataa gtattctttg agtgggaaaa
     6601 caccttttgt tctttttcaa tggctatttt ttggcatagt ttgcattttg atagttccta
     6661 taaccttttt tataacttct cttaccactc ccaagatctt agctgttgac ttgttgatgt
     6721 tgtttttttt aagtggaatt atttttgttt ctttcatgag aataatgtat gtaagtatgc
     6781 acttgtttga agcaagatat gcagtcgaaa cactagactc cctttattta gaaatgaagc
     6841 aagaccaact aaaatatggg aacgagacac agattaaaaa ttgtgacatt gcatttgaaa
     6901 atgtcagctt ttcatacaaa gaagagtgtg ttttagaaaa tttcaactta gttttagaac
     6961 aaaaaagaat ttatgcatta gtaggaaaat caggaagcgg aaagagtacg atagctaaac
     7021 taatttcagg tttctacaat gtggataagg gacagattaa aattggtggt aagcctttaa
     7081 atcactattc aaaagacaca attattaaca caattgcttt tgtctttcaa gatacgaaac
     7141 tattcaatca gagtatttac gataacgttt ctatagcaaa tcctcaagcg ccagcaagta
     7201 aagtatttga cgcgctaaga ttagccggtt gcgaatcgat tttagaaaaa tttcccgaaa
     7261 gagagcatac cttaattggt agtaaaggag tttatctgtc aggaggagaa aaacaacgta
     7321 tagctattgc cagagcaatt ttaaaagatg caaagattgt tattatggat gaagccagtg
     7381 catctattga tccagataat gagtatgaac tacaaaaggc ttttcaatat ttgatgaaag
     7441 aaaaaacagt tattatgata gctcatcgat tatccagcat taaatctgta gatgaaattc
     7501 ttgtcttgtc agaaggaaaa atagtagaac gtggaaatca taatgaatta atggctaaac
     7561 atcgagtgta taaacaattg gtcactaatt atgagaatgc aaatgattgg aggcttgaca
     7621 atgaaaaatt tttataaaaa aaaatttgct ttaactgatc agggcgcaga agctttaact
     7681 aaagcttcta tttctagttt ttttgtatac tgcattaata tggtaccagc attcattata
     7741 atgatgctga tagacgaatt agttttagaa aatgcaaagc cccgttggct ttattttgca
     7801 gtatcgtttg ttactttgct tttcatgtat tggttgttag atagggagta tgaaaactta
     7861 tataatagta cttataaaga aagtgcgcat ttaagagtgc aaattgcaga cgatttgtca
     7921 aatttaccat tatcctattt ttcaaaacat aatttatcag atttatctca aactatcatg
     7981 tctgacgttg aaggtattga gcatgcgatg agtcatgcaa tacctaaagc cggcggtatg
     8041 gctctgtttt tcccttttat ttcagtgatg cttttggttg gtaatgtcaa aatgggatta
     8101 gctgttattt tgccaacgtt atttagtttt gtcttaatct tgttatcaaa gaaatcccaa
     8161 acgaaagcca atactaaata ttacgatact ttgagagaaa actcggaaga atttcaagaa
     8221 actattgaat tgcagcaaga gattaatagc tttaatctat ctaaaaaagt tcaagacaga
     8281 cttttcaaaa aaatggaaga gagtgaaagg attcatttaa aggtagaatt aagtactttt
     8341 tcagtcatgg ccttatcctc tattttctca tatgttagtt tagcagtagt cattctagta
     8401 ggtgttcact tactgttaac gggtgaagtg actatactct acgtcgttgg ttacttacta
     8461 gccgcaataa aaataaagga ttccttcgat tctatgaaag aagcagtgct tgaaatattt
     8521 tatttagctc ctaaaataca gcgaattaga gctatgaagg aaaccagtat ccaagaggga
     8581 tcagatagtc ctctgaaatc ttttgacatt gagttgcgag atgtctcttt ctcatatgat
     8641 aacaataccc caattttaga tcatatctct tttacggcta aacaaggaga agtgactgcg
     8701 ttagtaggtg ctagtggttc cgggaaaact agtatcttga aattagtgtc tagactgtat
     8761 gattatgatg aaggctgtat tttaattgat gggtatgata ttaaacgtgt atctccagct
     8821 tctttgttta gcaaaatcgc tattgttttt caagaggtaa ctttgtttaa tacaagtatt
     8881 ttggaaaata tacgtattgg aaatagtcaa gcgtcagatg aggaagtgaa aaaagcagct
     8941 cgtttagcta actgcgaaga ctttattgaa aaactacctg acggttatca tacattggtc
     9001 ggagaaaacg gaagttcact ttctggagga gagcgacaac gcctatccat agcacgggct
     9061 tttttgaaaa atgcgccaat tttaatttta gatgaaatta ctgccagttt agatgcggaa
     9121 aatgaaaaac gtattcagga aagtttaaat cgtttaatac aagataagac agtactaatt
     9181 atatcgcatc gcttgaagtc tattgaaaag gtaaataaaa ttgtagtgat ggatcaagga
     9241 aaactagttg atcaaggtac tcataatgag ttgtatcgaa ggtcagaaat atacaaaaat
     9301 ctcataaaga aaactaaact atctgagaaa ttcgtctatg aaaaagaagc tcaatcacga
     9361 tgatgaaata ggctttaaat taacctacaa tttttgggat atagttataa ttctttttca
     9421 aaaagaataa ttttattaaa cagcatctaa ttttgaatac acgaaagaat cagtatgatt
     9481 gaattagttc acttaatgtc tattcttata atataaggaa taatcaaagt tttaaaaaat
     9541 tggcgataaa ccagttaaac ataaaagatt gaataaaaag ttatagtttc tttcatattg
     9601 ccgttttaag tagaggaaaa gctacttaaa acggtatttg tgtttgattg gactaataaa
     9661 agtgaattct tatttttcag agcctaaact gcatattctt ttaaatgatt tgattatctc
     9721 tatgtaccga taaattcgtt ttcaaatagt tatctattca tatgagtttt atttcatgtt
     9781 atttattgtt gacaagagca actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt
     9841 acgatttgag gtgtctaaat caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg
     9901 aaaattgcta aagcaaagaa acaaatggcg atgattgaaa agtatgcaga taaacgtcag
     9961 gaattaaaag ccgcaggaga tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg
    10021 aatcgtctga ggcttcgtga tcagacggat ggtcgtccaa gagggtatat gcgaaagttc
    10081 ggtatgtccc gtattaagtt tagagaactt gcccatcaag ggttgattcc tggagtaaaa
    10141 aaagcaagtt ggtaaaagga ggaaatatca tggcagtacc agcaagaaaa acatctaagg
    10201 cgaagaaacg gttaagaaga actcatcaaa ctgtagtaaa accagaaatt tcatttgacg
    10261 aagcgaatgg tgattataga cgtagccatc atgtatcttt aaaagggtac tataatggaa
    10321 agaaagtaat taaaggagct tcaaaatagt ttaatatata acttttagga gggaatcaaa
    10381 atggaaatta tttacccacc tttagttgaa gaaggattaa agtattatct tgaaacgacc
    10441 cagcaatcgc tagataaatg cactttttat cgttcgatgg tagagagagg aattattact
    10501 gaaacgggtt taccgaccca acaggcaatt gaaaatggat tagtcaaaga ttactatgag
    10561 gaccaaggct tatcatttga tgatttttta agaatctatc caatttttga agagtatgac
    10621 gaggagttat ttcaatgtat cgatggttat tgggagatac ctgttgatat gaaagaaaat
    10681 ttagtttcac aattggaatc tggcgagtta acttttgaag atgcacaaca aattcaagca
    10741 tatcttgaag atcgataagt attgaaggag gaagatttat gcgtcaaaat attattttgg
    10801 aatgtgtcga aactggagaa cgtctgtatc tcaccagcaa aaataaacgg aataaccctg
    10861 aacggttaga attaaaaaag tattcgccaa aattgcgcag acgggcaatt tttaaagaag
    10921 tgaaatagct aagtggtaaa aaggtgacaa aatgggcata ccagttacaa tcgtttctgg
    10981 gtttttaggg gctggaaaaa caacgctaat caatcaagcc ttgcaagtct caccctaccc
    11041 cagggaagaa attattataa ttgaaaatga gtttggtgaa gttgggattg atcacaaatt
    11101 attagttcat agcgaggaac gggtgttaca actaaataat ggatgtatgt gttgcagtct
    11161 tcgcagtgat ttattagcaa tattgatttc tttactggaa acaatagaag aacatcaaca
    11221 accaatttca caaatcatca ttgaaactac aggtatagca gatccccaac caatcattca
    11281 aacactttta acagcgcctc aactaagagg aagagtatat atcgatagtt tattgactgt
    11341 tattgatagt gacagttttg aacttattga aagagaacca caagccctta agcaacttgt
    11401 catggcagat cgcatttttg tgtctagaaa acaaaacttg aatttatctg cactaaaaaa
    11461 attacagaga gaaatcaaaa gtattaaccc tttatccgat attcgaatgt tcacacatag
    11521 tgaaacgatt caaaaaaagg atttttataa tttgaaaaaa tttaatcatc ccttagtttt
    11581 tgaaagtgat tcatcagaaa atcactataa tggcaaatac tatcatcaac accatcaagt
    11641 acatgagttt aaatcaattc tcttaacttc agatcaagac ataaaaaaag attttttact
    11701 ccaatggatt gactggctga ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt
    11761 gattgcttta caagagcagg attttatagt tgccatgcaa gaggtaaacg aacaggttaa
    11821 ttttcaatac accactcgac aaaaagacca aaccactatt attctaatcg gaaaaatgtt
    11881 ggatgaagaa aggatcaaac agagttttga taaattactg gcggaaagca aataatgata
    11941 agtagtgatt gggggtaatt tctaatggag aaaacggata tatctagtgc atatcgacga
    12001 cttaaaagcc cgaatattaa aactagaaaa agggcattaa aaattatcaa agaacataaa
    12061 cgaaataaaa tgaagaaatt agcataatta aggaaggaaa aagaatgaag aaaatcagtg
    12121 taggacttat tgggatagca gctctaggat tattgggagc ttgctccagc actaatgatg
    12181 caaaagtatc gaatgataaa gatggaaaat tagaaattgt aacaacattc tatccaatgt
    12241 atgatttcac caaaaacatc gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg
    12301 gatcagaacc tcatgactat gagccctcag ctaaggatat ggcaaaagct catgatgctg
    12361 atgtttttgt atatcataac gaaaacatgg aatcatgggt accaaaagct aaagaaagtt
    12421 ggaagaaagc tgggcctaat gtagtagaag gaacaaaaga catgatttta ttgccaggta
    12481 gtgaagaaga agatcatgat cacggagaag aagatcacca tcatgaacta gatcctcata
    12541 cgtgggtttc acccaaaatg gcaattaagg aagtttcaaa tatcaaggat cagttagtta
    12601 aattgtatcc gaaaaaagca aaagtatttg agacaaatgc tgaaaagtat ttgactaaac
    12661 tgaaacggtt ggatgctgac tataccacta gtcttaaaga agcgaaacaa aaaagttttg
    12721 tgacgcaaca cgcagccttt ggatatttag ctttggatta tggattgaaa caagttccta
    12781 ttgctggact aagtcctgaa gaagagccat cttctggtcg attagctgaa ttaaaagagt
    12841 atgttaaaaa aaataagatc aattatattt actttgaaaa gaatgctaat gataaaattg
    12901 caaaaacttt ggccaacgaa gctgggataa aattggaagt actgaatcct cttgaaagtc
    12961 ttacaaaaga acaaatggat aatggtgaag attatgtttc agtaatggaa gacaatctaa
    13021 aagcgttaga aaaaacaact atggttgctg gtgaagaggt agttccagaa aaagaagcga
    13081 aggatgaaaa gacagttgct aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat
    13141 tgagtgatta tacaggcgaa tggcaatctg tttatccgtt gttaaaagat ggaattttgg
    13201 atgaagtttt cgattacaaa gcaaaaatca ataaagacat gacagctgcc gagtacaaag
    13261 attattatac tactggctat aagacagata ttgatactat caacattaaa gacaacacca
    13321 ttgattttgt agtcaacggt gaacatcatc aatacacata caaatatgtt ggatacaaaa
    13381 ttttgaatta tgaaaaagga aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag
    13441 gtgctggtcg ttttaaatat attcaattta gtgatcatgg aattgcacca agtaaggcag
    13501 agcatttcca tattttcttt ggtggagaaa gtcaagaaaa actctataat gaattgcata
    13561 attggcctac tttctatcca gcttcattgt ctaaacatga aattgctcaa gaaatgatgg
    13621 ctcattaaaa ttcaattttt ggaggaaata catggtaaga aaagtattat tgtctatgga
    13681 ttccaagaat attgatgctt atcggcgtta tcgatttgca aagcaaatga gattaaatgg
    13741 gagtacatta gctgatattg ctgcaacttt acatgttagt attgacacag ttcatagagt
    13801 attaaaacgg gatcctgatt gttgttttat caaggtcaat actggaagaa gtagacgttt
    13861 agcaaaacgg cataatagct tggccaatta attgataata ccagagtaga tcatttttcc
    13921 cttatttgga tttgatctgc tttttattat gtcttgaatt gtcaaatcaa gcgcaacact
    13981 ttatcaaaga agacttcata aggtgttctc caatctaaac actttctagg tctgttattc
    14041 agtttttcaa tcatctgatt tatcgttgag ttatctaccg tagctatctc tttcgatttt
    14101 ggcaagtatt ctcgaatgag tctattcgta ttttaatttg ttcattgttg ccaagggcat
    14161 gaggatctgg aaaataaaag ggcatattat tcaatgcttc tgtaactgcg aaatgattgg
    14221 agaattcttt tcctctatct ggagtgatcg ttttgaccgc ctcatttgga atactgctta
    14281 aaagttcaat cattttctcg ctcacataag cggctttctt tctggggacc ttctttcctg
    14341 ctaaaaggaa gcatgatttt ctgtctgtca aagtcactaa acaagtacca ccagttttgc
    14401 cagctacaat atcggctttc caatgaccaa aatcacttct atcatctgcg gattttggac
    14461 ggccatggat cgaatgagta aggcaaatct ttccacgaga ctcgacatga ttttttgtat
    14521 gtcttgtttt tcctcgatgg tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg
    14581 gttctgtatc gaaaagatca ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa
    14641 agtccatatg ttcatgcttc aaacgattcg agatttcctc tggcgaccac tgaaaatcga
    14701 caaataaatg acgaacaatt gaccaaactc acggatcatt tagaatgtga taacgtccgc
    14761 atttttttct tcgataagca taattttttt ccgctttgga aggtgaatag tttttttctt
    14821 gtttcttctc agttcatgta aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat
    14881 actaatagaa aatccttctt cgtgaagcag aaagattctt tctcgttcga gtatagtaag
    14941 atgacgatac tggctcataa ttattttcct tctaggagtg ttctcgacaa acttatacta
    15001 gttggatatc gattatgact catttttatg tgttgcactt aaagtgtaaa ttcaaggtat
    15061 agaaagaggt tccctatgaa aaaaatacct attacaatct taactggcta tcttggttca
    15121 ggaaaaacga tacttctaaa tcatattctg tacggtgatc acggaaagga aattgctgtc
    15181 attgtaaatg acatagggga agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt
    15241 aaaagaactg gagaaaaact tgtttcgatg ccgaatggat gcatttgttg tacattacgc
    15301 gaagatttga ttgttgaact agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt
    15361 attgaagctt caggaatcag caaaccaatt ccaattattc aagcaattat attagctaaa
    15421 aaccctttag acattgattt gacccaacga ttagagattg gtgccattat tatagtagtg
    15481 gatgtaaaac gtttttggga taatttcgaa acgggtgaaa agattcagga aagaaaagct
    15541 gaaggtaatg tagttgaaga agaacaggat attcgtggct tgttaattga tcaattcgag
    15601 tgttgtaaca tattactttt aaataatagt gacttagtgt caaccaagac gatttacaaa
    15661 attaacggtc taattgctaa gttacagtcc aatgccaaaa taattaggac aattaaaggt
    15721 gaagtagact tatcgaaaat tctaaataca aatcttttta atatagatga gacttcctat
    15781 tcagtgggct ggttcaaaga gttagagctt ggctatcaaa atcatattcc tgaaacagaa
    15841 gaatatggta tctcaagttt tatctatcga aatcggttac tccttgacgc acaaaaattt
    15901 gcccatctta cggataaaaa ttttcctggc aatattacaa gagcaaaagg gtttctttgg
    15961 tttgcccaac atagtgattt ttcgacgatc tttgaaacat cgggtagcta taaaagtatt
    16021 gaatcattaa attattgggt agctagttta cctaaaattg agtgtcaaat gattatacaa
    16081 aaaaatcccg aattgaagga agcctggcat ccgttgtttg gtgaacgcat taataaacta
    16141 gtttttattg gtatgaagat ggatcaacaa tgtattaagg aacaacttga gggttgttta
    16201 actgattctc ttaatgcaga taatttccga aaaatttcac catttccatg gaaaaagtct
    16261 aatatgttta gccagatatc gttaatgaaa tttttaagaa aagctggtgt caagataggc
    16321 gatgtcataa ttattggcat tttgattatt acctctttta ttcccttatt tgtattctct
    16381 atagaaaagg agaatgaaac aactgaagat gctatcaaat atgccatcgt tcgaattgat
    16441 ggcaaggaaa tggatcgctt cgatttggat aagatcaatc ataaactcgt gaattatcat
    16501 cctgcgaagg gacaatataa tattgttgag attaaagatg gaagaattcg agttaaagaa
    16561 gataatagtc ctgatcaaat cgcggttaga actggatgga tctcaaaacc cggtcaaacg
    16621 agtatttgtt taccccataa gttagttatt agtattgaga aaaaagaaag cacagattac
    16681 tacatttact aatctggaaa tagatgagga ggtttttaat aagaaaaaga tattattatt
    16741 aataatagca ggggctttat taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt
    16801 gacaacaggc acaagtatct ttaacgggac ctgcaatggt ttagttaaca gtcggttaga
    16861 ttacttattt tggctcttcg tcaatgagtg ttggtgaaga aattcaatag aataatcgag
    16921 atactcacac ggctagacag ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag
    16981 ttgggtagta ttcttagttt tcttaaaata ttttcctcga tataaaaaga ttcgttctcg
    17041 gtagttttga aagtttctat agccataggc aattcgtttt agtactttga tatggttatt
    17101 caaacattct atagggccgt tggaataggg aacgcgcaac gctcgtttga tttctgtttg
    17161 gtatttcttg aaggtcgtga acactggttg gtagtgctct ggcaactcct tgtagtcttc
    17221 ttttaggagc gcgacgaatt caggaacatc ttgtcttctg ataacggaaa gaaaatcctg
    17281 gtagacttcg tagcctcgct ttaaggaatc atcataagat aatagccgat cgactatctc
    17341 tgcttctgtc agatgatctc gaaatgacgg acgccagtag cgatgctgac ctttcaattc
    17401 ccacgcattc ttttgaagta gtttccagta tttctttaag tgcttcgacc gacgatcccc
    17461 ttttttaaag gtcttcatta cttgaattcg gtggttggta aaagcccgac caatgtgttg
    17521 gctaatatga aagcgatcgg cgacgattcg ggcattcgga aaacactctt tcaccaatgt
    17581 gcgatagggt gtatagaaat ctgaaaccac tagacgcact tgttcacgga cttttatagg
    17641 gtagcgcaag aaatagtcgc gtaaaggatt gagttgccga ttttccacaa tatcaatcag
    17701 ttgatgggtc ttgccgtcca tcatgacaaa gctcatggct cccgagacat ttttcactga
    17761 tttgaattca tcgaaacaca ccacttctgg taacagctgg ctcaaagaaa tggttttagg
    17821 ccgaaggctg cgaagtattc gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat
    17881 caaagacata gaaagaggtt cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc
    17941 aatggagcag cgacgatcac taactgaatc ttctgctaaa aaagtctttc cacagtgttt
    18001 acatttgaag cgctgtttgt taatccgcag aatggtcttg tagttactga catcattcaa
    18061 taagatggat acttttttcc agccctattt aatgatcgtt tgttcatttt gatagtggcg
    18121 atgaaagcat tcctccggtt gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc
    18181 attgattcgc tctttgcgaa aggcatcttc atgaaagata agatttaggt ctagaatatc
    18241 tagtaactct ttggtaaact ctgaatagga catcttgaaa cactcctttg aatggttttc
    18301 gtcgacttta ttctaaagga ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa
    18361 gtattggcaa cagaaggcgt caccaacact agatattata gatcccttat tttcttcgct
    18421 tcaaattgtc aaggagtcat cataaatttt gtatggattc ttttgtttgg gtagtagtct
    18481 tctaaataat cttggattaa caccttcatc tctattttac ttttatattt gttggagtcg
    18541 attaattctc atttgattga tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc
    18601 ccagtttggc tcactgaatg tgtgatgtgg tggtcgttca acagctcttg ataaataaaa
    18661 gaccgatgct ggctacctct atcggaatga attaccgtag gcaattctcc ttaataaatt
    18721 tgtaatggac ggacgacaat ctcttttttc atgtgtgaat ctaccatata gtaacgtaga
    18781 atacgagttg ctaagtcaat ataggtggac aaatagaggc gaccgtcatt acatggaatg
    18841 taagtaatat ccatcaccca aacagcatca atttggttct gttcaaatta tcgattcaac
    18901 atttttttac catttctttg attgtttttt ctctaccatg agaagatttg cgtcgatgaa
    18961 atcctcttgt gcataaattc atttcacgca taagaacagc tacaacacgt ttattgattt
    19021 cgattccttc atcgtaaagt ttttgagtga tccctggaga tccgtatcga tctttgtgtt
    19081 cgtagaacac tcgtcttcct cttttagtcg tttattttcc tgttcaagaa tgtagaaatc
    19141 tgctttatca ggtaatcttt ttccttgtcc agcaaaagca tttttcccgt atcgacgata
    19201 ttctgaaacc catcgggaaa ttgttggacg agagatattg tattcttcag caattttctt
    19261 ggtagttact tttttctcaa ggatatcctg tgaaatcttt agtttaaatt tacgatcata
    19321 tgtttttctc aatagagttc attccttccg gtaacgtaac tgatagtcaa gaaaagtgta
    19381 tcatagccca acttagtatc agggtttgtc aactaaactg tggaagttga tagataagag
    19441 tttttaacaa ccaaaattct cttgactatt tttgaaatca gacaaatttt gattggacac
    19501 atagttgaat aaatctaccg tcgttacgga aggtaaatcg catgcttttc aattctagag
    19561 gagaagtcct cgttgaagta ccatagccca gttctggatt cgacctcctg cccacttcat
    19621 acgtaaataa agtaatttta ggagtgagac tgtgacaaaa gttattttta agctaaaaac
    19681 gaattaaaaa acgttgataa ttaaaatcat tcgttttaga ttctatgaat cattatagaa
    19741 cgctgtaaaa tcgatacttt ataattataa agaagatagt ttcaaattta ttgttcgaaa
    19801 tttgaaacta tctttttact tatgtcacag actcccttag ttgagttatt ctttaatttc
    19861 gaatttcgag atagctagat atattgcaag catacattct tgataacaat cttgatcata
    19921 ttttccagtt ctccaggatt gcttatgaat caaagagcta aatcttttga gaattgcctc
    19981 aacagcttct tcatttccag cctttgctaa aatgaaatct tcttttaaag aattcataaa
    20041 tctaccttct tcccatggac ttataatcta tttctttggg aaggaggaga acgagcaaag
    20101 aagcatacaa aattagttat tatgcagata ttaaaccagc attttttgaa tttttttgta
    20161 aagtcgatgt tttaaatttg tcgcaccctg tcttgtaatc cccagaacct ttccaatttc
    20221 tttatcagtt ttatcaaaga taaatttttc gataagaaaa atttgttcag gttctttcaa
    20281 caaaggaagg atttttgcta attttatatc ttcaacaagg agagtcacat ttaaaacatt
    20341 aatcggttgt ttaaatttta tagtatcttt agtaaacaaa tatggtggga gttgatcaag
    20401 ttgttcatgt ttttgaattt cttcttgttt tttataataa gtattagcgg tgttcaaaat
    20461 gaccttttca aagaatcgaa tcaactgggc atttacgcgt tcgtcgttat ctgacatatt
    20521 gcacacctcc tccattaaga tttaaaggga aaagaggcaa aaaacacgaa aagaagctaa
    20581 tgcaattatg ctgcgctgcg aataacttct ataagctcac atgcattgat aggactgctg
    20641 atgtttattt tcaaaaatgt ttttttagtt gataatggat agttatcttt atacttagaa
    20701 aagaaaattc atatatcttc ttaaatatca aacatgtcct tatggctaag aaaatgtctt
    20761 gagcttttta cgtataataa atcttttcta aaacctcctt ttggataata ttcttattcg
    20821 tgtatagatt gtatttttta tttcttcccc cttcataatt aacaactaga aaagagttcg
    20881 aatagacacg tatttgaacg aaaattttta ttggattccc aaattggaat atttagaaac
    20941 gaaaaattcc tattatctgt atagtgctta gataataacg gatcataata attattgtct
    21001 ttgaactaga tatatgtaaa tagtattcat catgatgaaa aatgatgagt tctcggtgat
    21061 aaaatgtact ttttacggtg gaatatacat gaaagcgata agtggatatt ttataatgaa
    21121 gtaagttagc ttattttgac agaggaatag tattttagat aacgtataaa atactattta
    21181 atgatgtaga aaggaaagag agaaacaaaa gtgttttcag ataaagaagt cttattaaag
    21241 tctcttcatg tttgtttaaa gttacctatc gtggttttta attccgaatt cagtattata
    21301 aaagagtatc gctcagatag aactacttat tttttttatg attataaaaa gttactaact
    21361 gataatatta aaaaaactaa agatttccat ttctttactg gtgattttaa tgagatgttt
    21421 cttctataca tgtttaaaac cagatattac ttatttggtc catttagagc aaataatatt
    21481 gataaagttt tttttaaact aaaaatgaac aatttgaatg ttgcaatggc tgatagagaa
    21541 cgactctata attcattgca aaatttgact ttgtattctt taggagacat tcgagatatt
    21601 ctgattttag ttcattattt ttttactgga aaaatagaag atctattcca tgaaccgtta
    21661 attgaatata cagaaaatct aagtaaaact atcgagcaaa tcaaaataga taatctgtta
    21721 tcacaaaatt acgatccaga aatatatctg tttctttatg aaaataaaat actggaatat
    21781 gttaaaaatg gagatattcg aaatttagaa aacatggttt ttaatctgag taatggaata
    21841 attccaagtg ttagtggaga tacgattcgg tctgaaaaaa attattctat cattgttttt
    21901 gaaaagttag cacaaacgtc tataacatta ggaatggata taatagaagc gtatcaaagt
    21961 agagatgctt tgatacaaga aaacgaatta gctgtatcgt tacctgaggt cttaaaagtg
    22021 agggattctg ggattgttta ttatactaag gaaatcggaa aaactaagat agagcatctt
    22081 tctcccttaa tttcttctgt cgttcaattc atcggcttaa atatctataa aagaattact
    22141 gtaaaggaaa tagctaatta tttttctgta agtgaaacta aattaagaga aagctttaag
    22201 aatgaaatgc atattacgat ttataattat atctctaagc gaaaaatttc tacggctaaa
    22261 ataatgttga aatcaaacca tactatcagt gaagtttctt taggtttagg attttcagat
    22321 tcttcacact tttcgagagt attcaaaaag tacgcaggtg tatctcctaa gcaatatcaa
    22381 ttaggtttag tggacaattt caacaacatt agctgagagg ttttatttta ttatgagact
    22441 atatcattta cgtagtttgg aggagttgag gaaattggat tttaacaaaa aatttttttg
    22501 ggaaatttct gatttgagtt gtttcgtaga atctgagcgt ctaagtaatg gtaataatag
    22561 tgtgttaaat gaatatgaca aagtagtaat taataaagta aaaagtttat gtgaagaagc
    22621 tagagaatct atcagtcata ttaatggtag caattttaaa caaacattat ttaatctgat
    22681 taagctagat gctaaaatat caagttattt attctttttg atacatgatg aatttatgga
    22741 ttatcaaaaa ttggatcagt taattgaaag agaaagctgg gaagattatt acgtgggtct
    22801 aactttttca gaaaagttaa acaactacaa gttaatagat tataagtatt tgagtgaaag
    22861 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
    22921 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
    22981 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
    23041 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
    23101 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
    23161 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
    23221 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
    23281 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
    23341 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
    23401 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
    23461 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
    23521 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
    23581 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
    23641 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
    23701 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
    23761 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
    23821 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
    23881 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
    23941 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
    24001 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
    24061 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
    24121 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
    24181 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
    24241 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
    24301 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
    24361 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
    24421 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
    24481 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
    24541 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
    24601 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
    24661 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
    24721 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
    24781 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
    24841 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
    24901 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
    24961 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
    25021 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
    25081 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
    25141 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
    25201 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
    25261 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
    25321 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
    25381 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
    25441 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
    25501 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
    25561 caatttcaga tgtctataat aaggacttcg gtaatgcaag tgttgatgca aacactggta
    25621 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
    25681 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
    25741 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
    25801 tcaccgcagg
//
LOCUS       DACCDF010000036        25377 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCDF010000036 DACCDF010000000
VERSION     DACCDF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.036"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(2..1990)
                     /gene="treP"
                     /locus_tag="IVK51_001485"
     CDS             complement(2..1990)
                     /gene="treP"
                     /locus_tag="IVK51_001485"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP6431086.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLVYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            2213..2926
                     /gene="treR"
                     /locus_tag="IVK51_001486"
     CDS             2213..2926
                     /gene="treR"
                     /locus_tag="IVK51_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP6431087.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            3003..3449
                     /gene="msrB"
                     /locus_tag="IVK51_001487"
     CDS             3003..3449
                     /gene="msrB"
                     /locus_tag="IVK51_001487"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP6431088.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            3619..4221
                     /gene="ruvA"
                     /locus_tag="IVK51_001488"
     CDS             3619..4221
                     /gene="ruvA"
                     /locus_tag="IVK51_001488"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP6431089.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            4234..5235
                     /gene="ruvB"
                     /locus_tag="IVK51_001489"
     CDS             4234..5235
                     /gene="ruvB"
                     /locus_tag="IVK51_001489"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP6431090.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            5264..5842
                     /locus_tag="IVK51_001490"
     CDS             5264..5842
                     /locus_tag="IVK51_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6431091.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKVLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            5894..6376
                     /locus_tag="IVK51_001491"
     CDS             5894..6376
                     /locus_tag="IVK51_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP6431092.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIAIEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            6493..6867
                     /locus_tag="IVK51_001492"
     CDS             6493..6867
                     /locus_tag="IVK51_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431093.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IVK51_001493"
     CDS             complement(7667..9019)
                     /gene="brnQ"
                     /locus_tag="IVK51_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP6431094.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            9166..9756
                     /locus_tag="IVK51_001494"
     CDS             9166..9756
                     /locus_tag="IVK51_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP6431095.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(9884..11233)
                     /locus_tag="IVK51_001495"
     CDS             complement(9884..11233)
                     /locus_tag="IVK51_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6431096.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11401..12141)
                     /locus_tag="IVK51_001496"
     CDS             complement(11401..12141)
                     /locus_tag="IVK51_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6431097.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(12154..13746)
                     /locus_tag="IVK51_001497"
     CDS             complement(12154..13746)
                     /locus_tag="IVK51_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6431098.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(14314..14610)
                     /locus_tag="IVK51_001498"
     CDS             complement(14314..14610)
                     /locus_tag="IVK51_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431099.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            14786..15511
                     /locus_tag="IVK51_001499"
     CDS             14786..15511
                     /locus_tag="IVK51_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP6431100.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            15504..16346
                     /locus_tag="IVK51_001500"
     CDS             15504..16346
                     /locus_tag="IVK51_001500"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP6431101.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKAVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            16349..16870
                     /locus_tag="IVK51_001501"
     CDS             16349..16870
                     /locus_tag="IVK51_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP6431102.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(17123..17686)
                     /locus_tag="IVK51_001502"
     CDS             complement(17123..17686)
                     /locus_tag="IVK51_001502"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP6431103.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            17937..18206
                     /gene="rpsO"
                     /locus_tag="IVK51_001503"
     CDS             17937..18206
                     /gene="rpsO"
                     /locus_tag="IVK51_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP6431104.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            18394..20520
                     /gene="pnp"
                     /locus_tag="IVK51_001504"
     CDS             18394..20520
                     /gene="pnp"
                     /locus_tag="IVK51_001504"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP6431105.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            21030..21296
                     /locus_tag="IVK51_001505"
     CDS             21030..21296
                     /locus_tag="IVK51_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP6431106.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            21440..23431
                     /locus_tag="IVK51_001506"
     CDS             21440..23431
                     /locus_tag="IVK51_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP6431107.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            23785..25086
                     /locus_tag="IVK51_001507"
     CDS             23785..25086
                     /locus_tag="IVK51_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6431108.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
BASE COUNT         8173 a         4807 c         5202 g         7195 t
ORIGIN      
        1 actaagattt tacttcagct ttaccgactg cttcgcccgg gcgaacaagt cctgattggt
       61 caagagaaag tcctgcaact ttttcattat tggtcaaagc aacaataatg tcagttttct
      121 tgccagcttg ttctatttct ggcaaatcca tttcagcaat cagggtctct ggtgtgacaa
      181 ggtatccttc tttcacgaat acattgaaag gaacaccctt tagttcaaca gtatctaatc
      241 ccatgtgaac aagtacttcc agcccctcat tagaaagaat accaatggca tgttttgttt
      301 caaagacact cgtcacttta ccgtttactg gtgcatagac tttgccgttt gacggttcga
      361 cagcataacc gtcacccatc attttttgtg agaaaactgg gtcatttact tcagttattt
      421 ccttgatggt ccctgcagcg actgcgaata atgtttcttt tgttgcaact gccgtaccag
      481 ttgctgaaac aggttcgaaa gactgtggcg ttgctgttgc gccatttgct gtttgaagtt
      541 gtacatcagc agcattttca ggaacagcag gatcaatctt attcaaaatt ccctgacgtc
      601 taaagatgat cgtcaagcca aatggaatga tgacagcaat gatcattgcg atgatgaaag
      661 gtacccatgt ttctgcttgg atagctagta ttcctggcaa accgcctact ccgatcgcgt
      721 tagctcgaac acccatcaag ttagcaaaca tgccagctaa tccagagccg accattgcag
      781 cgacgaaagg atagacgtat ttcaaattga ttccgaacat agcaggttca gttacgccta
      841 agtaacaaga aatcattgct ggaatcgaga tttgttcttc ttttttattc ccacgatgta
      901 agaagatgat ggctaagact gcagagcctt gggcgatatt tgataaagca atcatcggcc
      961 ataaattcgt tgatccgaaa tctgcaatca gctgcatatc gatggcattg gtcatatggt
     1021 ggagaccagt aatcactaaa ggagcataca ggaatccgaa tacggcacta aacaaccagc
     1081 tgatagaaga cgtcaatcca gtatttacga tatttgagat ccagcttccg actgtccagc
     1141 cgataggacc taaaataacg tgtgcagcta aaacagttgg taacaatgaa aacaatggaa
     1201 caaaaatcat cgagatcgac tgagggatat atttgcggaa gaagatttct agatacacta
     1261 acataaaccc tgccagcatg gctggaataa cttgcgcttg ataaccaatc atctggactt
     1321 gagcaaaacc aaaatcccaa aatggaatat ctgctgcggc tgtggaagct acactataag
     1381 catttaataa ttgcggagaa actaacgtaa ttcctaaaac gattcctaat atttgtgtag
     1441 tacccatctt ttttgcaata ctccaagtga tacctactgg aagaaaatgg aaaattgctt
     1501 cacccggtaa ccacaaaaag gcattgacac cattccaaaa cttcgagtgt tcaacgattg
     1561 ttccaccaag actttcaaac tggatacctt caagtacatt tctgaatcca aggatcaatc
     1621 caccgacaat gattgcagga atcagtggtg taaagatttc tgctaaaacg gcaatagcac
     1681 gttgaacggg atgaaggttt tgttttgcag ctgcttttcc ttgttctttg gaaacccctt
     1741 caactccaga tactgcaacg aagtcgttat aaaaagtaga aacatcgttt cctatgatga
     1801 cttggaactg tcctgcattc gtgaacatcc cttttacgct aggtatatct tcgatcgctt
     1861 tttcgtccgc ttttcctgga tcattcagga caaaacgcat acgagtcgca caatgactaa
     1921 ctgcagcaat attttctttt ccgccgatat catgtaacag cttctcggca tctgcttgat
     1981 attttgccat atcaataaac ccctttctct taacttgtct atgcaagttg tttcttcatt
     2041 tactatactt tttttcggag agagttgcaa gcgttttatt aaaaagtacg agagaaaatg
     2101 atatcgtttt aaaaagctta aaataagctt ttcttaaaaa taggaacatt gaaatacaaa
     2161 aagagctgta gtatagtgaa gaaatgatgg aagaacgagt gagggaaaaa acatgaataa
     2221 gttcaatgaa atttttttag atttggaaca gaaaatttta gcgaaagaat atcctccgca
     2281 ttcgctttta cccagtgaga accaattgat caaaatttat ggtgtttcac gagaaacaat
     2341 tagaaaagcg ctgaatcttt tgactacgga aggttatatt caaaaaaaac aaggaaaagg
     2401 ttcgatcgta ctggatcgga atcgcttcga ctttcctatt tctggattga ctagctataa
     2461 agagctacag gagacccaac gtattccaag tgaaacgatc atccatacgt tagaagaaac
     2521 agaagtgaca aaagcattga acgagatcac tggctgggag atcggtgcgc ctgtttggca
     2581 tttgatacgc gaaagaaaaa tcaatggcga agtagttata ttagacaccg attatctatt
     2641 aaaggaaatc gttcctattt taactccagc acaagcacaa ggatcgatct atgaatattt
     2701 tgaaaatgag ctttctttga cgattgctta cgcgcaaaag gagatcacag tagaagaagt
     2761 aacggaaatc atgaaaacag cgatggatct tcacgatgac acttatgcag tagtagtcag
     2821 aagcctcgtt catctggaag atacccgctg tttcgagtat tctgagtcgg ttcaccgatt
     2881 agataaattc cgtttcgttg agtttgcccg acgccgaaaa gtgtaaatag ttcaaaagaa
     2941 agcaaattgt tggcatctgt ccactttggc gctatgatag gatcacaaag ggggcatttt
     3001 taatggataa accaataaga ccagaaaaag aagaactgaa agaaaagtta tcacccatgg
     3061 cttatgcagt cacacaagaa aatgcaacag aacgaccatt tagcgggaaa tatgatgatt
     3121 tttacgaaaa ggggatctat gtagatatcg tcagcggtga accgttattc agctctgcag
     3181 aaaaatatga tgcaggttgt ggctggccat cattcagcaa accaataacg aaaagacaag
     3241 tgcgcgaaaa agccgatttt tctcatggca tgcatcgaat agaagttcga agcaaagaag
     3301 cagattctca tctcggacat gtgtttaccg atggaccagt agatcaaggt ggtctacgtt
     3361 actgcatcaa ttctgcctca ctgaaattca ttccatatga aaagatggat gaattaggtt
     3421 atggggaatt caaatcactt gtagaataat ggaaatacac aaagagcggt acaatacggg
     3481 gatttactcg atcgtccgct ctttttttat tcaagcgatt gctatttgaa agaaacgctc
     3541 cccagactcc ttttttcaca aggtgaaatg tgctacaata aagggaacgt atgtgtgcaa
     3601 aagaaagagg actagaatat gtatgaatat ctgacaggtg tggttacctt tatcaacccc
     3661 tattacatag tagtagaaac caatggtatc ggctaccaga tcgcactagg aaatccttac
     3721 cgctacagta gcaaactaaa tcaaaaaata aaaatttatg tccatcaggt aatacgtgaa
     3781 gatgcgcatc ttctgtatgg atttgattcg ctagaagaaa agcaactgtt tttgcgatta
     3841 gtaagtgttt caggaatcgg tccgaaaagt gcccttgcaa tcatggcaag cgaagaccac
     3901 tccggtttga ttcaagcagt agaatccagt gatgtcactt atttgacgaa atttccagga
     3961 gtagggaaaa agacagcaca acaaatgatc ttggatctaa aaggaaaatt cggtgagctg
     4021 agtatcgata caccttttag tctatttgat gaagcaaaca caaaagacgc tacagctcta
     4081 tctgaagcaa tggaagctct ctcggctctt ggttacagtg atcgagaaat caaacgtgta
     4141 gaaaaacaat taaaagaagt agataatcag acaacggatg agtatctacg ccaagcactc
     4201 aagctaatga tgaaaaaata aggaggagac caaatgacag atgaatactt gttatcacca
     4261 gaatctggcg aaaatgaact gagtttggaa aaatctcttc gaccacagct gctttctcaa
     4321 tatatcggac agagaaaagt caaacaagaa cttcagattt acatcgaagc tgccagaaat
     4381 cgggaagaac ctttagatca tgttctgttg tacgggcctc ctggattagg aaaaacgacg
     4441 atggccatgg tcattgctaa tgagatgacc gtaaatattc gtacgacgag tggccctgct
     4501 atcgaacgtc cgggtgactt agtagcaatc ttgaatgaac tggaagcagg ggatgtcctg
     4561 tttatcgatg agatccatcg attacctaga gtggtagaag agatgcttta ttcagctatg
     4621 gaagatttct atgtagatat catggtagga caagggccaa ccgcacatcc ggttcatttc
     4681 cctcttcctc cctttacttt gattggcgcg acgacacggg caggtatgct ttctgcacct
     4741 ttacgagatc gatttggcat catttctcat atggaatatt atgacgaaca ggatctgcag
     4801 gagatcgttt tacgttcttc ggatattttt caaacagaga tcattgaaga aggggcattg
     4861 gaaattgctc gccgatcacg aggaacgcct agaatcgcta atcgcttgct caaacgggtt
     4921 cgtgattttg cgcaagtcca aggagacggg aaaatcgatc gtcctatagc ggatcaggca
     4981 cttactttgc tacaggtcga tcaggctgga ttggattatg ttgaccagaa attgttgcga
     5041 acaatgatcg agttatatgg cggcggacca gtagggttaa gtacgctttc agtaaacata
     5101 ggagaagagc gtgagacggt agaagatatg tatgaaccgt atctgatcca aaaaggattt
     5161 ttaaagcgta cacctagagg gcgaattgct actgcttatg cctatgaaca ttttggttac
     5221 gactatcata tatgattttt gcttttagat tggaggaaaa tgaatgagtg atacaacacg
     5281 tacgaaacaa ttgatcaaaa acgtattgat tgatttgtgc caaacgcaat cttttcgaaa
     5341 aatcagcgtc caaaaaatcg caaatgaagc aaaaatcaat cgacaaacat tttattatca
     5401 ttttacagat aaatacgatt tgcttcgttg ggtctattgt gaggatgcat tgcattattt
     5461 agatccacaa tgtctttcac ttgataattg ggaagagcaa gcattgaaag tgctgaaagc
     5521 aattgctgag aagaaagaat tttatgcaaa tactgtgctt tctgacccag aaattttaca
     5581 aaaagagttt tctcaactga tccaaaaatc atttatcaag atttttgaac aaatggatga
     5641 agaaagacaa ctttctgcaa gagacaaact attttatgct cgtttttttt cttacggttg
     5701 cagtggtgta ttagtcaatt ggatcacgaa tgaatatgaa gaaacaccga tggaaatcgc
     5761 tatgcagctt ttccgtttag ccaaggatac ggaattatat tcttatcgtc tttatgcaaa
     5821 agaagagttg gaagaacagt aatagaaaaa ttttttgttg gaaaagcaat aatcgctaaa
     5881 aggagatggg gaaatgacaa aaattttatt tgtttgtttg ggaaatattt gccgttcacc
     5941 gatggcagaa ggcttgctga gaaaacgaat agcaatagaa ggaagggaag cagaattttt
     6001 tgtggattct gctgctacaa gcacgtatga agttggaaag acaccgcacc ctggaacgaa
     6061 aaaaatatta aaccaagaaa aagtagatat gacgaatatg gttgctcggc aaatcacacc
     6121 ccacgatttt gaaacttttg attggatcat tggcatggat caagaaaatg tagaagaact
     6181 gaaacgaaga gcaccacgct cagcgcaggg gaaaatccat ttgtttttaa gtagcattcc
     6241 tggaaaagaa aaagaaaatg ttcccgaccc atactatacc aataactttg atgagacata
     6301 tcacttgatc acagaaggcc tgaatcattg gtggaatatt tggattgacg aaaataaaat
     6361 aacaaacaat ttgtaaagtt gacgctttct ttttaaggcg atgttagagc atttccttgt
     6421 ttttttgtaa aatgttaggt aaaataaaat taattaagta acactttctt agctaaagga
     6481 ggagtgcatt cgatgaacga agagacagta tcgccaatga agaagcttac gaagttagtt
     6541 actcatactt cgtatcaact ttttgattct tttgtaaatg acactgaaga acatgctaag
     6601 aatgtacgat tgcaaggaaa gtttattctt gagctgcaaa aagctgctac tgaacatagt
     6661 ctagttatct tacaagtaag agcgaatcgt ttgagtagca agtatgaaac gatctcaggc
     6721 tgggtggcga caaaaacggt cgaaaaagac cgagtgatga tccgcctaca aaacgaagag
     6781 cagcagatcc ggatggttcc tgtagaacaa attgaaaaaa taacaatcct ttcaccagca
     6841 ggggaacagg aacgattatc aaaataattt ataccagata gaaaagcacg tatgagcttg
     6901 taccatcaat cattactaat caaaaataac cgaatgcttt aagctgatat cagtaaaacg
     6961 gaacgtttta ggatgagtat cacagcatga cagcattggg ttgattagtt gaatcgattg
     7021 gatggtacaa gtttttttga taaaaaaagg tcctgctagc cggggagcta gcaggataag
     7081 gagttaaaaa tgaaaaagtg ttgttagggt tgtttgttgg tatgcttata taatagtcac
     7141 tagttgtgaa tattttgtga cgaaagtttt cgaaagttgt taagtttttt tgcgaaaact
     7201 atttttatat catatatatg tctaaaatct atattataaa agaaatgttg tttgatcagc
     7261 ttctcttcgg tattagttca aaacaaaacg gcaaagtata tggagctact ttgccgtttt
     7321 gtttctctaa tactcaggtc agtttataga cgctcaataa acggtgttca aaagccagtt
     7381 tctcggtatt aagtcaaatt tatgcaaaaa tgtgagaagc cattttcaca aaatatttct
     7441 taatacttga aactaagcgg ctttttcacg acctctcaat aaacggtgtt caaaagtcag
     7501 tttctcggta ttaagtcaaa tttatgcaaa aatgtgagaa gccattttca aaaaatattt
     7561 cttaatactc gaaactgagc ggctttttca cgacctctca ataaacagtg ttcaagagac
     7621 agcctatcgg caataaggct gaacgtctct ttcacaacct cttttatcaa tctgctactt
     7681 cttctttctt agcgaaactc caaatcagtc cgataacaaa accgataact gctggtacga
     7741 tccaccccat ccccaatttg aaaaacggca agtagctttc cgcaacatat aaaagatttt
     7801 gaacaatagg tgtttgtttg atagtttctg ggcaagcgtt caacccatca aaaatcgaag
     7861 caatcaatgt aaaataagta gtcattcgat aaacagccgt acgctgctta aacaaaggac
     7921 caactaacac tagtaaaatc agtgtcattg ctaatggata gatgaacatc aaaacaggca
     7981 acgaaaattg aatgatattc gtcaagccta cgtttgccgc taaacaagga aggatactag
     8041 caagtgcaac aaagaatgcg taatttcttt gcggaaacaa atctgtgaat gtttctgcaa
     8101 aagctgtaat caaaccaata ccggtcttca agcaagctaa tataacgata aatgctaata
     8161 agatactgcc ataagtacct aaataatgat cagcaatctg agccagcgtg atcccgccat
     8221 tttcacttag agcaaagcta cctaagctca tagcacctaa ataagcgagc aaagtataaa
     8281 tgattcccat caatataatg ctgatcgcac cagatttgat cgtgtcttta gcgatttcag
     8341 caggttttgt tacacccatg ttttggattg ttgtgacaat gatgatccca aatgctaaag
     8401 cagcaagcgc atctaatgtg ttatatcctt gagtaaaccc tgtgaaaaaa gcatgctctt
     8461 gataatttgg ctgaataggc gcttgattta cagaacctag tggattcaaa aaagcaagga
     8521 gaagcaaagc acctaataaa accaaaaatg caggattcag gaactttcca acataatcta
     8581 atatctttgt cggttttctt gaaaaccacc aagccagcac aaaaaataca atactgaaaa
     8641 ttgctagata tagcgtctgt ctgctaggat cgataaatgg tgctaaacca atctcgaaag
     8701 atgtggttgc aaggcgaggt aaagcaaaaa acggtccgat aactaagtaa agcaagatgg
     8761 taaaaaccat cgcatatgtt tgatttatcc gttttgctaa ttcatataca cctgagcttt
     8821 tcgatacacc aatggcaatc acccctaaga agggaagacc gataccggta accaagaaac
     8881 ctagattcgc ccaaaaggta gcagcgcctg cttcttgacc caaatgaacg ggaaagataa
     8941 gatttccagc accaaaaaat agaccgaaaa gcatggagcc tataaaaaga tagtctttaa
     9001 atgataattt ttttgtcata cataactcct ttacggtttt tttaaaagta acaaaatttc
     9061 gaaaaatatg caacaatttt cagaaaattt cctatataaa acgacggtta tttaataacg
     9121 taagtgataa aataaaagcg ctgaatcatc tgaggaggaa tcatcgtgac aaagtcgaga
     9181 ttagaagcat ttactgatgg agtggtagcc attgttttga gagttcttgt attagagatc
     9241 aagatcccta atacatctag ctttcattct ttatgggtca tccgaaatac attattagct
     9301 tataccatca gctttatatt tgttgcggtg atctgggtga accatcatcg tatatttcag
     9361 atggcagaac gaattaacta ccgagtggtc tggtcgaata tctttttgct cttttggctg
     9421 actttatgtc cttctgttac tagttgggtc gggcgtaacc ctgaagcttt ttggcctgag
     9481 atggcttatg tcttagtata cacgatgtgg agtttttctt ttggtatctt gatgaggcaa
     9541 atcatgaaag caaataaacc agattcccat gtcgtgagag ttttgtcaaa ggaccgtcga
     9601 agtcttatct ccatgatgat taacttatgc ttgattgccg gtgtcttttt tgtaccgttg
     9661 atcgggttat tcggacgttt ttttgtatct ggtatttgga ttttttccta taaaaaagca
     9721 gatgcctatt atcagcggtt gtttccgggg aaatagaaaa gaggctggga cagaagcttt
     9781 taactccaag aaataagccg aaatttcaca aaaatttaag aagcaatttt cgtaaattcc
     9841 ttcttatttc tcgaagctga ccacttctgt cacaactact ttcttacttc ttacgttcat
     9901 atttcatatg atagattagt aatagaagca agaaccacac aggtgttagt aataaagcga
     9961 ttctcgtatc ttttcctagg aacaagacga cacacacaaa ggccatgtaa agaaatacca
    10021 gataattact aaatgggtac aacggcattt taaatgaatg agcttttttg ccgaggatac
    10081 ttttacgata tttcatgtgc gtgtagacaa gcatactcca gataaacaag aaacaagtag
    10141 tggcaatact gcttactagt gtgaatactt cactaggcat cacgtaattc aagataacag
    10201 aaatgaaaat gacgatagta gaaaatgtca aagcattccc tggcacatga tgtgttgtta
    10261 actttttgaa ttttttaggg gcactgcctt cttgcgctaa agaacgaagc atacgtcctg
    10321 tactatatat tgcactgttg caggcagaag ctgctgcact taatacgact aaattgatca
    10381 aggaagcagc aatcgtgatc ccaatttccg aaaatacttc tacaaatgga ctgttatctg
    10441 gattcaagct gttccatgga tagattgcca taatgacaaa aagagatcct aagtaaaata
    10501 agatgatcct gataggaata ttattgatag cttccggcaa cactttttca ggattttctg
    10561 tttctccagc gactaatcca actaattcta tcccagcaaa agcaaaggtt accatttgga
    10621 aagctaagat aaatcctttt gcaccagttg gaaagaatcc gccatgagac catagattcg
    10681 tgatactggc tgtgccaata tctgtcgtat agtgggtgag gatcatataa gctcctacga
    10741 ggataaaggc aatgatcgct acaactttga tcaatgcaaa ccaaaactct aactcaccaa
    10801 acaatgagac agccaccata ttcaagcaaa gaagaagacc taatgcaatc agttcaggaa
    10861 gccactgagg aacaccaggc gcccagtagc gtacatacat tccgatggct gttaaatcgg
    10921 ccattgcaat cgatacccag cagaaccaat acgtccagcc agtgatgaaa gccgcttttt
    10981 tgccaagata ttctgcaaca aaatccagaa aagaatggtt attggtgttt gataacagaa
    11041 gttcgcctaa cgcacgcata atgaaaaaac agatgcatcc agtaataaga taggcgagca
    11101 atatcgaagg gccagctagc tggatagact ttccggaacc taaaaacaac cccgttccaa
    11161 tcgctccgcc aatagaaatc agttggacat gtctattttt cagtccacgc tgcagttctt
    11221 gctcttcatt catttttaaa tcatcctaac taaaaaagtt taattacttc catattaaaa
    11281 gagttttctg aaaaacacaa ctaagattcc aaaattttca gaaatttacc ttttatgagt
    11341 aattcataaa aaagaactgc ggtaaacacc gcagctcttt gtcccaatca gctattgatc
    11401 ctaagcattt aaaatacgtg aaaggaattc ttttgtacgt tcttctttcg gatggacaaa
    11461 gatatcttct ggtgtacctt cttctgcaat cacaccttta tccataaaaa tgacacggtc
    11521 agatacatct tttgcaaatt ccatctcatg cgtcacgata agcattgtca agccagtatg
    11581 tgctaagtct ttgatcgtat taagcacttc gccaaccatt tcagggtcta acgccgaagt
    11641 aggttcgtcg aatagcaata catctggatt cattgataat gcccgagcaa ttgcgacacg
    11701 ctgtttctgt ccaccagata attgtgctgg tttggcttcg atataacgat ccataccaac
    11761 tttttccaga ttttcgattg ctactttttt ggcagtttct ttatctcgtt tcaggacagt
    11821 tgtttgacca gacatacagt tttccaagac attcatattg ttgaacaggt tgaaggattg
    11881 gaaaaccata cctagattcg ttcgatattt aggtaaatta tatttaggag ctaatacatt
    11941 ttctccatta taaatgatct cgccgcctgt tggtttttcc agtaaattga tacagcgcaa
    12001 gaaagtagat ttaccagaac cggaagaacc gatgatcgtc acaacttcac ctttattgac
    12061 tgttacgctg atatctttca atacttcatt ttcaccgaag ctttttctta gatgtttgat
    12121 ttcaataatt ttttcattca tgattcttcc tccttattcc tgcaaatttg aattgtcgat
    12181 ttcttcaagc ttggtataag ctgaaggacc atcgattttc ttttctaccg cccgcaggat
    12241 tctggtgatc gtaaaggtca tgactaagta gatgatcccg acgatcgtaa atgtttggaa
    12301 gaattggaaa ttcgcaccgg aagcagagtt tccttggaag aacaaatcag ctacgccgat
    12361 tacacttaaa actgctgtat ctttgatatt gataacaaac tcatttcctg ttgctggcaa
    12421 gatattccgt aaaacttgtg gaataacaac ttttctcatc gtttggccat gggtcatacc
    12481 aatggcttgc gcagcttcga actgcccttc atctactgca aaaatccccc cacgaacgat
    12541 ctctgtcatg taagctccag tattgagaga gacaataaac aaagcggcaa tcgttcggtt
    12601 caatgaaatg ccaaaagcga gtgccagacc gtagaagatg accattgctt gtaccatcat
    12661 cggtgttccg cggaatactt cgacatagat agaaagaagt gcatttccga ttttttggaa
    12721 gaagcgagtc agtgcatttc ctgattcagg taccgaacgg aagacaccaa tcagtaatcc
    12781 aagtgtagta ccgacaactg tcccgatcaa tgcgatgaac aaggtaagtc cagcacctcg
    12841 taagaacata ttgccgtatt gtttccaaat cgtagtgaaa tcagaaatga tgccaggttt
    12901 ttcttcacta tcagttgcag caggctgatc tttgactgct tggtccataa ttttggttcg
    12961 ttcatcgtta gagatgccag aaagaatctg gtttactttt tggatatccg ggtcgccttt
    13021 acgcatacca acagaaatct gaacatcttc cgggtttgtc tggaagccat ctgcaccgct
    13081 gaattcaagc atttccaagt ctttgttcac actagttgcc gttacacctt ccggacgttc
    13141 gctgacataa ccatcgatca ttccagaagc taaagctgtt ctcattgctg agaaattatc
    13201 cattgcttgt tgtttctgca catctggtat ctgatcgatc acagtatagt ggaatgtatt
    13261 taactgagca gtaatctttg ctccagaaag atctgctaaa ctttttgctt ttgcatattt
    13321 gctatctttt tgaactacta ctactaattg tgattcgtaa taaggatcgg taaaatcaat
    13381 ttcttttcgt cgttcagcag tagggctcat tccggcgatg atcgcgtcga tcttgcctga
    13441 ttgaagggca ggagccagac catcccattt cgtttggacg atgactaatt ttttccctaa
    13501 accatctgct acttttttag cgatttgaac atcataaccg ccggcgtaac tattttggcc
    13561 ttgaattggc aaggcattat tctcatctgt ttgttgagac cagttgaagg gtgcataccc
    13621 agcctccata cctacgcgaa actcatcgct cggtgttttt tcatctgcat ttgccagaga
    13681 tcccacactg aatagtgaga agacaaaagc aaatagtacg gcaactgaaa ccatattttt
    13741 tttcattttt ttctcccttt ctgattatat gatacacgag cggtctagat gaaatgcggt
    13801 aaagctttat ctgaaaaaat aaaaagcgac cgatttatag atataaaaag gccgctaaaa
    13861 ttccataagg tgataaacct ctcacaaaac atagcgcaac ttagcatcac tgctaagaca
    13921 gtccgcaagc tcttaacttg cgtcccaaca tacactccaa gcaaaaaagt gcacatttcg
    13981 gcgatcctcc ctattcctgc ttctccatgt ttgcttcact ccacagggtt aatgattctc
    14041 gcgacctcta cctcattgat tgaggcatct cgtattcagt tatttataca aaaaaagttt
    14101 acgatactcg agaggctttg tcaatctata actgctgttt tttgaaaaaa tactgataaa
    14161 tatagaaatc tttactttta ccgaaaaaac aatcaaaatg tgaatcatct attcattgca
    14221 aacgatagta taattataca caaaaatgta tgtaatacaa taaaaaaaga actagaacag
    14281 aaaatttcct gtgtccagtt ccatctcgat catttaagct gccgctttct ttggctcacg
    14341 tcctaggatc gcttgcaaaa gaacaacgac gccataaatg gctaatatta gataaatcaa
    14401 acttgagtat tgggtgattc cccagcccca tgttcgatat acggctaatg taatggctaa
    14461 aacaattgcg gcaatcacgt tgaataaagc aaaagcccaa agatttccat tttctttgcg
    14521 tacaagataa aaaacagaga aataaacgct taacgctaac caggcgatac aagccagaat
    14581 aacgccccaa taaataaaga atggtaacat atcgattcac ctcactttta tacagcagta
    14641 ttgtaacacg ttttcattct tttgtcagtg aacattgtga taaattgttc aaatgcccat
    14701 aacacttttt tattcccatt tttctgactt ttcaacaata aatttgttcg agtatgggct
    14761 ataatggaaa gtgaggtgac aaaacatgaa acaattaaca gtagacgaag cagcaaaaga
    14821 gatattagct tatcagcaaa ttacattttt gaccggtgca ggggtatcca caccttctgg
    14881 agtgccagac tatcgatctt tgtcaggaat ctaccacggg atggagcagc cggaatactt
    14941 gctgagtcat caagcaatgg tgaatgaacc ggataaattc tatcaatttg tcaaaaagat
    15001 ttatcatcct gaagcaaaac ccaatatcat tcatttggaa atggcacatc tagcgaaaga
    15061 aaaaaatgtt tgggttgttt cccaaaacat cgacggtttg cacgcaaagg ctaaaagccc
    15121 gcaactcgtc aattttcatg gaagtttgta tcaatgtcat tgtcgaaaat gtcaacaaac
    15181 ggtagactgg aaagaatatc ttcacagcga caaacatcaa gcatgcggag gacaaatacg
    15241 cccagacatc gtgttatatg gtgaagggtt tcaagatgga gtcatggagc aggcggctta
    15301 tgccgtcagc caagcagagc tgatcgtcat tgttggaacg agttttcaag ttcatccgtt
    15361 ttgtgatctg gtacagtttc gccagccgtc agccaaaatc ttagtaatca atcaaacgcc
    15421 tgtttattta gcggagcctt atacgtttat tcaagaagac ggagcggttg ttttcaaaaa
    15481 aatacaagaa ttaggagcag attatgactg aattcaaaac cgaaaaagaa aaaatgattg
    15541 caggcgagct ttattttgcc aatgatcctg agctggttgc cgaccgtatg tttgcaagaa
    15601 gtcaaagcca aatcatcaac caagcagaat cagcggaact gcgatcccaa ttgttgaaag
    15661 agacttttgg ccgtacaggg aaaaaaatct atatggagcc agttatcaat tttgattacg
    15721 gatataatat atttgttggg gagaattttt atgcaaattt caattgtaca tttttagatg
    15781 tcagcacgat tgaaatcggt gataactgca tgttcgctcc gaatgtccag ctttacacgg
    15841 cgacgcatcc actacatcca gtgaaacgta acagcggtct ggagtacgca aaacccatta
    15901 aaatcggtaa taatgtttgg ttaggcggag gagttatcgt cacgccgggt gtcactctag
    15961 gggacaacgt agtagtcggc gcaggttcgg ttgtgaccaa atcttttccc gacaatgtag
    16021 tgattgcagg aaatcctgct cgtatcatca aggcagtaga ggaagaacta acagaagagt
    16081 ctcttgaaac attacgtcat aagatcgata tgatcgatcg agagttggtt gctttattag
    16141 aaaaaagaat ggatacggta actaaaatag gacaagtgaa aaaagaagaa aaccaagcag
    16201 tttatgatgc aaaaagagag cagcaagtat tggataaagt tgttagtctg ctgaaaaata
    16261 agggatataa ggaaacaata acagatacgt atgttgattt gatgaaacac tcgcgcgaat
    16321 accaaaacaa aatgaaggag gaataagtgt gatcgaagga gagacacggc gaaaacaaat
    16381 catgcaaacc ttggcaaata cagaaaaacc agtgagtgct agcaagtttg cgaaaacttt
    16441 tggagtcagc cgtcagatca ttgtaggcga tattgcttta ctacgagctg caggtaaaag
    16501 tatcgtggca acagcaagag gttatatcct cgaaacagaa gaaaccaata ctgggcacat
    16561 cagtaaaatc gctgtccagc atggaaaaga tcaaacagaa gaagaactga gactaatcgt
    16621 tgaaaatggt ggtgaaatca ttgatgtaat tgtggaacat cccctttacg gtgagttagc
    16681 aggaactttg cacatcaaaa cgccacaaga tatcgattcc tttatgagac gttatcaaaa
    16741 aagtaatgct actttgttat ccgaattaac aggcggcatc catctgcata cgatccgcta
    16801 cccagaaaag catatgctga aaaacatcaa acaaaaacta gctgaagcag gaatcctcta
    16861 cgaaggataa catcgtagct gctacttttt atcctagaaa ctattctatc atctctgaga
    16921 taaacaccca tatggaagaa ataagaaaaa ataagaaaag atgcgtatcc aatacacaga
    16981 attttttgga attgcgattt ctagatgtct atctaaattt cgattagtgg agaaacataa
    17041 aaaagggaag tgcagctttt gagcactccc cttttttata aagctttctt gtagcatagt
    17101 atcgaaggaa cctgccaatt ctctactcga tcaccaatac gccttctttt agtgcaaatg
    17161 gattttgatc gttgatgtgg tcatagaaca tcacaccgtt gatatggtcg atttcatgtt
    17221 gaacgacgat cgattcataa ttcttcaaac gaatcttgtg tttttcacca ttcatatcat
    17281 aataagacac agtgattttg gcatggcgaa caacatatcc tggtacttct cgatcaacag
    17341 ataaacagcc ttcaccttcc cctaaacaag catcttgaac agaatggctc aaaattttag
    17401 gattatacat aacagtactt agagaaggtc catctgcctc tgggtcagga cttggtacgt
    17461 gaacagcaat gattctttta gaaatatcta attgaggagc agctaagcca acaccgcctc
    17521 gtaagtgtag ttcttcagct ttgattggat cttggctgtt ttttaagaac tcaagcatct
    17581 cttttcctaa agagatatct tcttcgctta atggaagaga aacttctttt gcaacttcgc
    17641 gtaaggtcgg atttccttca cgaatgatat catccattgt aatcatggaa tatgtacccc
    17701 tttcttaaaa gactgtgttt caagtctatt ttcgcattat agtttaccac aaaaatggaa
    17761 aagtagtagt aagattgttt gaaaactacc ttttcttaat gattaactac ttgattttca
    17821 acccggtttc aggtaaagta tagctgtgtg ctctaggcac atgacctgcc acttggttaa
    17881 gcgaatcacc aacgttttac tcagtcgcag gggaataatc gaaagaggtg aaagaaatgg
    17941 caatttcaaa agaacgcaag aatgaaatca tcaacgaata cgctcgccat gaaggggata
    18001 ctggttctcc agaagtacaa atcgctgtat taactgaaga aatcaaccac ttgaatgaac
    18061 atgcacgcgt gcacaaaaaa gaccatcatt cataccgcgg cctaatgaaa aaagttggtc
    18121 accgccgtaa cttgttagct tacttacgta aaactgacgt tcaacgttac cgcgaattga
    18181 tcaaacgttt aggcttgcgt cgttaatttt agtcaaaaag agtgggattc acatgaatct
    18241 cacttttttt actcgtatat caattaagta aaattccgtg atcaagctag atggactcct
    18301 actaaccaaa tgaaagctgc tcaaaagacg gctttcggca gttttcattt gtttagtagc
    18361 gtcttgcttt cacgagaagg aaaaggagaa gttatgtcag aaaaacaagt attcaaaacg
    18421 acatggggcg gccgtccctt acaaatcgaa gtcggtcagc tagcaaaaca agcaaacgga
    18481 gccgttttag tccgttatgg agatacagta gtcttaagtg cagctgtggc ctctaaagaa
    18541 gcaaaagata cagacttttt cccattaacg atcaattatg aagaaaaaat gtatgcagca
    18601 gggaaaatcc ctggaggttt catcaaacga gaaggccgtc caagtacgga agcaacattg
    18661 actgcccgtt tgatcgaccg tccaattcgc ccgatgtttg cagaaggctt ccgtaatgaa
    18721 gtccaagtaa cgaacatcgt gatgagtgta gaaacagact gctcgccagc tatggcagca
    18781 atgctaggtt cttctttagc actatcaatc tcagatatcc catttgacgg cccaatcgca
    18841 ggcgtagaag ttggtcgtgt aaatggcgag tacgtcttga atccaacagt agaacaagca
    18901 gaacaaacag atatcgaatt gactgttgcc ggaacgaaac aagccatcaa tatggttgaa
    18961 agcggcgcga aagaagtttc agaagaagac atgcttggtg cattgctgtt tggttttgac
    19021 gctatcaaag agttagttgc tttccaagaa gaaatcgttc aagcagtagg gaaagaaaaa
    19081 atggaagtga ctttactgca agttgatgaa gtattgaaaa aagaaatctt tgatgcttct
    19141 tacgctacaa tgaaagcagc agtaatgact gaagaaaaac ttgctcgcga agataatatc
    19201 gagcaggtga agatcgatat ccgcgaagct tatgctgaga aatttgcagg tcatgaagat
    19261 gaagatcact tgttgaaaga agttaaacaa atcacagaag atcttgaaaa agatgtcgta
    19321 cgtgaattga tcaccatcga taagatccgt cctgacgggc gtaaattgga tgaaatccgt
    19381 ccattgtcat cagaggtcag cttattgcca cgtgtacatg gatcaggttt gttcacacga
    19441 ggacaaacac aagctttatc agcttgtaca ctcgcacctc taggagagca ccaaatcatt
    19501 gatggtttag gggtggaagt cagcaaacga ttcatccacc actacaattt ccctcaattt
    19561 tctgttggtt ctacaggacg tgcaggctct ccagggcgtc gggaaatcgg acatggcgca
    19621 ttaggtgaac gtgcgttagc acaagtaatc ccttcagaag aagaatttcc ttatacgatc
    19681 cgtttagtag cagaagtatt ggaatcaaat ggttcttctt cccaagcaag tatctgtgct
    19741 ggcacattag cattgatgga tgcaggtgta ccaatcaaag cgccagtcgc cggtattgcg
    19801 atgggacttg tttctgatgg tgaaaactat acgatcctaa ctgatatcca agggctggaa
    19861 gatcatcttg gcgatatgga ctttaaagtt gccggtacaa aagatggtat cactgcttta
    19921 cagatggata tcaagattca aggaatcaca gaacaaatcc taactgaagc attaactcaa
    19981 gcaaaacaag cacgtatgga aatcttagaa gaattgactt caacgattgc tgctccacgt
    20041 gaagaattaa gccaatatgc accaaaaatc gaaatgatcc agatcgaacc tgctaaaatc
    20101 aaagatgtta tcggtaaagg cggcgatacg atcaacggaa tcatcgatga aactggtgtg
    20161 aaaatcgata tcgatcaaga tggaaaagta agtattgctt cagctgatgc agaaatgatc
    20221 aaaaaagcta tcaagatcat cgaagatttg accaaagaag tcaaagttgg tgaagtttat
    20281 ctagggaaag ttgtccgtat cgaaaaattc ggtgcattcg tcaacttgat caaaggaaaa
    20341 gatggacttg tccatatttc ccaattagca aacgatcgtg tcaataaagt cgaagatgtt
    20401 gttaaactag gtgatgagat ccttgtgaaa gtaactgaaa tcgataaaca aggacgcgta
    20461 aatctgtcaa gaaaagcaat gttgaacgaa gatggttcaa cgaaaaatga agaaaaataa
    20521 tttaattcat aggaataatg tacttgtgat cgtgaaggtg acagggggac aataattatt
    20581 cgaaaaaagg ggactgtgac aaaagccgtg tcacagtcct tttttttcga ataaacggtg
    20641 ttcaaaagct tgcatctgcg gtaataagtc cagccaagtt aaaaacatta ggagctgttt
    20701 ttacttggct gttcttatta cttgatgcag aacagctttt ttacaacctc tccaataaac
    20761 gatgttcaat cagctgtccc tcgatattag ctcaaaaaac ggcaaaatat gaggaactat
    20821 ttttccgttt gtttctctaa tactcaggtc aaaccgctga tttcacaacc tctgattcac
    20881 ggtgttccag aagcagcttc tacggaaata agctgaacac ttttgttaca acttcttttt
    20941 ttctggagtt tcttggagca tatttttgga gtccttctga ataaagcgta aaataaaagt
    21001 gagaaatatg gataaaggag ggaaaggaga tgctgaattt tttatggtcg ttatttatcg
    21061 gcggtatatt aggtttgata gctggcgcaa ttttaggaaa ggaagtcccg ggaggcgtat
    21121 ctggaaatat cattatcggt tttttaggta gttggctcgg cgaattttta ctaggaccac
    21181 tgggaccgga actcggcggt ttttatttga taccagcatt attgggtgca attttttgcc
    21241 ttgctcttta ttcatttgtg gcaagacgta tcagagaacg tccaaagaaa gaataatttt
    21301 attcaaataa gttgacactt cagtgtgtta cttggataat tagaagcaat ttaggaaagc
    21361 gggtgtcatg gataagaata tcatctatca ttcaagctta cctaaaggat atccatataa
    21421 caagaaatga gggaaaaacg tggcaacaac gcacaatacc aaaaagatat caatggctgg
    21481 cctgcttgta gcgatgggag tcgtctacgg agacatcggg acaagtccgc tttacgttat
    21541 gaaatcgatt gttgaagata atggcggatt gtcaggaatc aatcctgatt ttgttatcgg
    21601 atcagtctca ttgatttttt ggacattgac tctactgaca acaatcaaat atgtcgtgat
    21661 tgcattgaat gcagataacc atggtgaagg tggaattttc tcactctaca cacttgtccg
    21721 aaaaggtggg aaatatttga tcattcctgc aatgatcgga ggagctgcct tactagcaga
    21781 tggggtattg acccctgctg tgacggtaac gactgcgatt gaaggcttgc gaggaattcc
    21841 gcagtttttt gaccggttcg gtaataacca gaacgtgatc gtcattatca ctttagttat
    21901 tatttttgtt ttattcatgg tccaacgctt tggtacagat attgtcggaa aagcattcgg
    21961 accaatcatg ttcgcttggt ttactttttt aggcgtaatg ggattaatga acttcagtca
    22021 agactggaca gttatccgtg cactgaatcc ttattatgct atccagctgc tttttagtgc
    22081 agataacaaa ttaggcttgt tcattcttgg aaatattttc ctggctacga caggtgcaga
    22141 ggcgctttat tccgatcttg gacatgtcgg caaacgaaat attcgtctga gctggcctta
    22201 cgtcaaaatt tgtttggtta tcaattattt tgggcaggct gcgtggttgt tgaatgttta
    22261 tcaaaaccct gaagcacagc aaatcaagaa cctgaatcca ttcttccaaa tgatgcctca
    22321 aagttggatc gtgatcggcg ttgcttttgc aacagtcgct gcagtcattg cttcgcaagc
    22381 attgattaca ggttcgttta cgctagtttc agaagcaatc aagttgaaac tgctgcctcg
    22441 gttaaagatc atgtatcctg ggaacagtat cgggcaaatg tatatcccag cagtcaatct
    22501 gattttatgg atcgcctgtt ctctagtggt tgtcacattc cgaacgtctc atcatatgga
    22561 agctgcttac ggtctatcga tcactgtgac gatgttgatg acaacagcac tgctttactt
    22621 ttatcttatc caaaatgggc attctcgttg gcttgcatat ttagttacct ttttcttcgg
    22681 tgcgatagaa gtcgttttct ttatctcaag tgttgtcaaa ttcttccgag gcggttttgt
    22741 agccgttctg attggtttga tcatcttagt cgtgatgttt atttgggaac aagggaacat
    22801 tattcgcgaa tcgtcaaccg aggatgtgtc tttgagagat tatatcccgc agcttagaga
    22861 attacgtgat gatacttctt taccaatcta tcagacaaat gtggtcttct tggttcctga
    22921 tatggctgaa gataaagttg gccgacagtt catctattcg attttagata aacggcctaa
    22981 aagagcacga gtctattggt ttgttcatgt ggaagtgacg gatgagccat atacgaaaga
    23041 atatcaagta gatatgatgg acactgattt tattgtccaa gtgaatctgt tcttaggctt
    23101 cagggtccaa caggaaatca acgtatatgt tcgccaagtc atccatgatt tgatgaaaca
    23161 aggccgtttg ccaaaacagc cacaaagata ctctctgaca ccagggcgag aagttggcga
    23221 tttccaattt gtcctgattg atgaagtagt ttccaatgtg acaactctag gaaaatggca
    23281 acgtcaaatc atgcaggcaa aactggcaat caaaaaaatc gctacctcgc cagaaacatg
    23341 gtttgggcta gagtacagcg aagtaaaaca tgaaactgtt ccgcttatca ttggaaatac
    23401 tcggaaaaca tggttgaaag agcggttgtg aaagtagcgc tttgacctga actaatgtcg
    23461 aatgtcagct acttgaacac cgtttatcag aggtcatgaa aaagctgtac tgcaacaaac
    23521 agtaagaacg agcagataaa aacagcttct catgtttttc atctgtttgg gcttaatgcc
    23581 gcagatgcaa gcttttgaat accgttttta tcagaggtca tgaaaaagct gtttagcaac
    23641 aagtaacgca atgattttga agtgataagg agatgtggct gaaaaatgcc gcatctcctt
    23701 ttttattcct aaatatgaat acgcattatt ttcaatcttt ttgtagaaaa agggtacaat
    23761 agaaaaagag aaaggcggga gaatatggtt atcaatttca ttattttatt tttgatgatt
    23821 gcaattactt cattttttgt agcaagtgaa tttgctttag tgaaaatcag acgatcaaga
    23881 ttggaacaac tagaaaagga aaatgtaaag aacgccaagc tagctcttca tgttacacac
    23941 catttagata actatttatc agctagtcaa ttaggaatta cgctaaccgg tttgatcatc
    24001 gggtgggtcg gtgaaggatc tgtcgcggct ttactagaac cagtgatcgg taaactacct
    24061 tttagttcgg ctattagcag tacaatctct gtggcgctgg gatttatttt agttacgtat
    24121 atcgatgtgg ttattggcga actgcttcct aagagctata gtatcgtcaa tacggagaag
    24181 gtcgtcttgt tcgtagttaa accacttcat tatttttata aagtaatgtt tccttttatt
    24241 tgggtattga atcattcagc tgcagggcta gggaagctat taggtgtgcg tctcgtatct
    24301 gaaggagaag agacattgtc tcaagaagag ctgacattag tcgcattgaa ttcttatgaa
    24361 aaaggagagt tgaccaaaga agaatatcat tatttggaaa acgtgtttga atttgatgat
    24421 accttggcaa aagatataca agtagaccgt acgtccatgc aagtattcga agcttcagaa
    24481 accgtcaaag aagcgataaa agcttcgatt gaacaaggac atactcgcta tccggttatc
    24541 ttagaatcaa aagacaatgt tttaggttat gtcaccttgc cagatttgat caagcaatct
    24601 tataaagatg accagctgac agtagaacag ttgattgaag agccgatcgt aacaactgaa
    24661 acgatcccta taaaaaaatt attgacgatc atgcgaaaaa agggaaagca tattgctatc
    24721 ttaaaagatg aatacggagg gacaagcggc ttagtgacga ttgaagatat tttagaagaa
    24781 atcgtcgggg aaatccgaga tgagacagat ttggacgaag cattgatagc ggaacagtca
    24841 gatggctctt atattatctc cggcaaacta acattagatg atttccagcg ttattttcat
    24901 gtagagattc cagagtttga agaaacgaat tttactacac ttgcgggctt tgcttctagc
    24961 cgatataaag aaataaaagc aggaacaatc attgagattg catcattccg tttcacggta
    25021 ttagaatacc agcatgcaca tatcgattat ttcaaagtag agtccacgga aagaaaaaca
    25081 gaataaaaga aaaaaggctg tgacattttt tgtcacagcc tttttcctac gattagtcaa
    25141 gtaaaaggtg caagaaaaat agctcgtaaa cgaaagcgac aaaatcaaag cacaccaaat
    25201 aaagccccgt tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt
    25261 tagttaatgg tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat
    25321 agcgtgcata catttcagaa gcttgttcat acaagcaact gtggcaacct tatcttt
//
LOCUS       DACCDF010000037        25010 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCDF010000037 DACCDF010000000
VERSION     DACCDF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.037"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            20..397
                     /locus_tag="IVK51_001508"
     CDS             20..397
                     /locus_tag="IVK51_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431109.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            1003..1617
                     /locus_tag="IVK51_001509"
     CDS             1003..1617
                     /locus_tag="IVK51_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6431110.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            2390..4129
                     /gene="yfcC"
                     /locus_tag="IVK51_001510"
     CDS             2390..4129
                     /gene="yfcC"
                     /locus_tag="IVK51_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP6431111.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            4286..5470
                     /gene="iadA"
                     /locus_tag="IVK51_001511"
     CDS             4286..5470
                     /gene="iadA"
                     /locus_tag="IVK51_001511"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP6431112.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5581..5784
                     /locus_tag="IVK51_001512"
     CDS             5581..5784
                     /locus_tag="IVK51_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP6431113.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5885..6547)
                     /locus_tag="IVK51_001513"
     CDS             complement(5885..6547)
                     /locus_tag="IVK51_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431114.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(6560..8062)
                     /locus_tag="IVK51_001514"
     CDS             complement(6560..8062)
                     /locus_tag="IVK51_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431115.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8610..10289
                     /locus_tag="IVK51_001515"
     CDS             8610..10289
                     /locus_tag="IVK51_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6431116.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            10416..10895
                     /locus_tag="IVK51_001516"
     CDS             10416..10895
                     /locus_tag="IVK51_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP6431117.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            11114..11794
                     /locus_tag="IVK51_001517"
     CDS             11114..11794
                     /locus_tag="IVK51_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP6431118.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11802..13133
                     /locus_tag="IVK51_001518"
     CDS             11802..13133
                     /locus_tag="IVK51_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6431119.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            13148..14110
                     /locus_tag="IVK51_001519"
     CDS             13148..14110
                     /locus_tag="IVK51_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP6431120.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLASGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            14221..14559
                     /locus_tag="IVK51_001520"
     CDS             14221..14559
                     /locus_tag="IVK51_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP6431121.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14638..15345
                     /locus_tag="IVK51_001521"
     CDS             14638..15345
                     /locus_tag="IVK51_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP6431122.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15448..16929)
                     /locus_tag="IVK51_001522"
     CDS             complement(15448..16929)
                     /locus_tag="IVK51_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6431123.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            17238..17774
                     /locus_tag="IVK51_001523"
     CDS             17238..17774
                     /locus_tag="IVK51_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6431124.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            17852..19222
                     /locus_tag="IVK51_001524"
     CDS             17852..19222
                     /locus_tag="IVK51_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP6431125.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            19289..19624
                     /locus_tag="IVK51_001525"
     CDS             19289..19624
                     /locus_tag="IVK51_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431126.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(19705..20562)
                     /locus_tag="IVK51_001526"
     CDS             complement(19705..20562)
                     /locus_tag="IVK51_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP6431127.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            20669..21184
                     /gene="tadA"
                     /locus_tag="IVK51_001527"
     CDS             20669..21184
                     /gene="tadA"
                     /locus_tag="IVK51_001527"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP6431128.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            21207..21291
                     /gene="ffs"
                     /locus_tag="IVK51_001528"
     ncRNA           21207..21291
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVK51_001528"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            21501..22886
                     /locus_tag="IVK51_001529"
     CDS             21501..22886
                     /locus_tag="IVK51_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP6431129.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTDIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            22923..23600
                     /locus_tag="IVK51_001530"
     CDS             22923..23600
                     /locus_tag="IVK51_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP6431130.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            23608..24372
                     /locus_tag="IVK51_001531"
     CDS             23608..24372
                     /locus_tag="IVK51_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP6431131.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            24374..>25010
                     /locus_tag="IVK51_001532"
     CDS             24374..>25010
                     /locus_tag="IVK51_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP6431132.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQ"
BASE COUNT         8155 a         4153 c         5282 g         7420 t
ORIGIN      
        1 aaaaaggtgt gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc tgcttgatat
       61 actcaagtgg ctgatggaag gaagcaattg gatatttgtg ttgccactat tgcttattag
      121 tctatttatc atgtttcgca tggtcagaaa aaaagaaacg tttctgattt ttatctggta
      181 tgcagcatcc gttggggttg ctaccttgat ggttgaattg gcgttaggta gttgggaagc
      241 ttgcgtattt gctttatttt ttgctgtact gtatctaatc atttatatcc catatatgaa
      301 aagaaagtct cggataggca gaaaaggtag acatacaact actttttcta gtacagattc
      361 tctcgaacaa agtagaagaa cgagaagaag acggtaatat ttagtaggta tacgcaaaaa
      421 aagataaaat aatggataaa ctattgagat gagagattta tttaattggt ggaaaaaagc
      481 ttttttcact tatataaaag aatctttttt aaaggaatgc ttgttgtatc gggataagcc
      541 attccttttt ttatgttatt tatatatata taaatcatac caatgttttt ttgtattttc
      601 ttgcttgcac ttggataaga gattacttac aataaattca gatagaaaca gctgaataaa
      661 aaaaggctcc ctgaccctta gttggcgcta agaatcaggg aatgaccagt cacacacata
      721 caccctgacc gatcattttg ccatagtcaa tgcagtgcat cgacctaatt tattgtacct
      781 aatctagggt gaaaattcta cccctatgta gatattttcc atggtctgca cgttgtttta
      841 tttgctatgt caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt tgaaacagca
      901 aagaccacat gtgttactgt ctaactactg tttttttatg tacttcctta gattaggcag
      961 ctgtttctat tcatgtcaag tatagctagg cgggagagat aaatgagttt atgttatttt
     1021 gactattatc accaattgga agagaaatta aggatagaag agccgctgat gcttcaagct
     1081 ggcacttgga tcgcgaaaca aattaatcaa ggtggtagat tgcagatata tgctagccgc
     1141 acactaagtg gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt accaagtata
     1201 ttgattgaaa atccagccgg aggtgtctat gaaacgctag aaggtgcagg acaggcaatt
     1261 attgatcagc tttctgtcaa atcagaagac atttttcttc ttctttcgaa tgaaggaaga
     1321 aacccggcaa tcatcgaact agctcagtgg atcaaagaaa atggtcatcc attaatcatt
     1381 gttactggat ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg tttgcgatta
     1441 tttgaatttg ctgatttggt tttggacaac cacgccaatt tacatgatgc agtcttgact
     1501 ttgccaaatc tagatccagc gatctgcgga acagcctctc ttgcaacgat ctttttgctg
     1561 cagcaagttt tgtattttac agcaaaacag ctagtccagg acacaagtgg aaaatagtta
     1621 tccacatgtt gacaaagttc cttaaaaaaa taatgttcca cataaaaatg gagcgtttga
     1681 aaaaaagaaa acgattatta atgaggatta acaacgacta ctttttttgt tttagttatt
     1741 ttaagatgga tcatctttga ttttaagctt gttttgttta aagtttgtta tttttataag
     1801 ttgtttcaca gttatgagaa atgagttata agaatatgta gtgtcagaaa aataaaaagt
     1861 agtggaaaaa gagacaagaa agaatccata tggattcttt cttgtctctt aatacacgtt
     1921 gatgaaagag tcttccccaa aactctttac aaagtaatta tagtacattt gttatcatta
     1981 ttgcaatgaa gtttacctga tataacaaaa ttgtccaaca ggataccttt ttctaataga
     2041 aataaaatac acaaaaattg aaggatagat aatggatatt tataagtgtt tattattttt
     2101 tttgcaatga aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga cgagattttc
     2161 tctaatctaa aaataaaaaa agaattttaa ggaaaaacag agaaataaaa tacttaatat
     2221 aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata gaaaaaagtg
     2281 attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc atttgattat
     2341 ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt tgaagaaaac
     2401 aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc atacgtatgt
     2461 cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc cagcaggaaa
     2521 gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta ctcgtactgt
     2581 tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg tgtttgatca
     2641 attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc cggaaaaagg
     2701 attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac tagatgaaat
     2761 ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg atacttctaa
     2821 aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct ttggtttctt
     2881 aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg tagggatcgc
     2941 tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg gagcgatcga
     3001 cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag ctttaccgct
     3061 attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag aggtaattcc
     3121 attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga tcgtagcagt
     3181 aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga gcccgtttag
     3241 cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga cattccgatt
     3301 gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat atgcagaaaa
     3361 aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt tccgttctca
     3421 tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat tgatcttatt
     3481 agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag gctattacat
     3541 tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca ttgctgttct
     3601 tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat ctggtgcagc
     3661 tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc ttgtcttggg
     3721 aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga tcgggacggc
     3781 gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc aaagtttgtt
     3841 caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa tcatggctcc
     3901 actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc agcttggttc
     3961 aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc taggtgttgc
     4021 acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat tcctgtttgt
     4081 tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt actggagacc
     4141 tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc attttacggt
     4201 aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct atcatcagtt
     4261 atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag tgtttgcacc
     4321 agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg ctgtagaaga
     4381 acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg agggaaaagt
     4441 actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag gagaaggcgg
     4501 ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg gtgtaacgac
     4561 agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt tgatcagcaa
     4621 agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt cgtaccgatt
     4681 gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg ataaaattat
     4741 cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt ttgaagagtt
     4801 cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag ctgggatcat
     4861 caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa tcgtagaaga
     4921 aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac cagaattatt
     4981 tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg ctagcgaaga
     5041 tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac tgaaacgatt
     5101 gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac aaggaagctt
     5161 gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg ccaaggcatt
     5221 gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa tcgccttacg
     5281 agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa aaatcgaaat
     5341 tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg atacagttat
     5401 cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga ctttcgaaaa
     5461 aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct gtttgtctgt
     5521 atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg aggaagcaca
     5581 atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg tgaaggaaaa
     5641 gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa agcacaatca
     5701 ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa agacaagttt
     5761 tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa gcaaataaaa
     5821 aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat cattatcctg
     5881 ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact tcatcacagc
     5941 tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt tcatgggcga
     6001 tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt ccagtaggct
     6061 catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg actcgctgct
     6121 gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca attcctacac
     6181 gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta gtgatttcca
     6241 ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa gcttggaaaa
     6301 taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt gaacgatctt
     6361 gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa agaaaagtcg
     6421 tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg aaattcagtt
     6481 ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat tgttctgtcc
     6541 gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat cttaatatcc
     6601 ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg gctaatagtc
     6661 ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa acagcaaatg
     6721 gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg
     6781 gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa
     6841 gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca actcctaatg
     6901 caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta cttctggatt
     6961 ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta atcataatga
     7021 tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt ttagaaaaac
     7081 ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt gattgtaagc
     7141 taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc attttatctg
     7201 gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt
     7261 catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag ttgaatttca
     7321 tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc atttcataag
     7381 tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg
     7441 tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg tcatctattc
     7501 cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt gcagttgctg
     7561 acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc attcctccag
     7621 caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca gaactagtga
     7681 tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa taactcttta
     7741 cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta agagaaaaac
     7801 tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt tcctgttttt
     7861 taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct ttttgtgctt
     7921 gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa
     7981 tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt gtacttttcc
     8041 atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt tgataataga
     8101 caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa catgaggttc
     8161 tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga aaaaagtatg
     8221 tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta gatgctacag
     8281 agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt tagtttattt
     8341 gttgtataat aatggggaac agctgaatac ttatataagt attctggcta tagcgtttcg
     8401 caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc aactgtcaga
     8461 acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat agtgattttg
     8521 catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga catctttaaa
     8581 cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc ccattaggcg
     8641 gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat atttttattt
     8701 tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc gtaatccctg
     8761 attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta acacatggac
     8821 atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta ccagtattcg
     8881 ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag
     8941 gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt gaaacagtag
     9001 tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat ctgaaaacaa
     9061 aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct atcccaatgt
     9121 atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta gccttactaa
     9181 gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa atagcagatg
     9241 aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta
     9301 atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga aaagttgtct
     9361 tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa aaacttcaat
     9421 taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat ccagaagaac
     9481 tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa
     9541 acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc actactacgc
     9601 caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat cgagctggtg
     9661 gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat cctaatgact
     9721 tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa ggagagtatc
     9781 gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac aaaaatatct
     9841 atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa
     9901 ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc ggaaacattg
     9961 ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt gtcactatca
    10021 gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga ttcgtttacg
    10081 taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca atcgttgaga
    10141 aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc cgagatcaat
    10201 taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca gtcattatgg
    10261 aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc tctctataga
    10321 gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag catccctcgc
    10381 tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac
    10441 gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat gtggtagaag
    10501 aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata tgtaaaactc
    10561 tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg aacgatcact
    10621 tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt cctggaatgg
    10681 attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt ggaataaaac
    10741 tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa gaagaagtaa
    10801 ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat ttagaaaaga
    10861 tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa gctaagcaga
    10921 gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct gtattatctg
    10981 ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt agcagctata
    11041 ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac agagaaaacg
    11101 cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa ttgaaatgga
    11161 aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga cagacttaac
    11221 tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg accacatagg
    11281 agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg ttgagattgc
    11341 tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg gaaaacatgc
    11401 gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct atgaagtaga
    11461 cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag aaggaaaaac
    11521 gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg cagaagaatt
    11581 cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc cagaagatgc
    11641 agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt
    11701 ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa
    11761 aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca aaacatgatt
    11821 tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta agaagcatcg
    11881 caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg
    11941 tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc aaattaaaaa
    12001 aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg gacgttttag
    12061 gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg atgactaaac
    12121 atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac cgggcggaag
    12181 tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga ttcaatacga
    12241 ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc acctcttatg
    12301 atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg atcaaaaaag
    12361 atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat ttgcagcaga
    12421 cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg cgttttccag
    12481 tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt ttagggaaat
    12541 cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa
    12601 tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc atgccggtcg
    12661 tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg aaagcgatgc
    12721 agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata tctggaaata
    12781 tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg
    12841 tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta cttagcgaaa
    12901 tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat atcttgattg
    12961 aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta gacattcgtc
    13021 cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg tttattgaaa
    13081 acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct tagaaagcag
    13141 gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg ataaaatcat
    13201 tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag gattagccga
    13261 actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg
    13321 gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt
    13381 catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc agttggcaga
    13441 aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc ttgtgtgggt
    13501 aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga tgtttcctga
    13561 atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg tcggagatac
    13621 agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt cgatattgag
    13681 aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt
    13741 cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag
    13801 aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag agacagcttc
    13861 gatcgtttca cttcctggag tgattgacga agtgctggct tcggggattt ttgttcagca
    13921 gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca atgaattggc
    13981 taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa aagatttagc
    14041 agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata agagcagaaa
    14101 aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt
    14161 ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag gagaaagaag
    14221 atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga tcgaggcttg
    14281 ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa
    14341 gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca
    14401 gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc
    14461 aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga aggatttgga
    14521 ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa ggtcttttca
    14581 cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga aacgaaaatg
    14641 tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat tcttggatta
    14701 gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata cgatcaagtg
    14761 agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca aagtcaacag
    14821 ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta caattcaggt
    14881 aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc tgcgataggt
    14941 gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt cccattgaag
    15001 attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt ccccttgatt
    15061 atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat tttcgctttt
    15121 tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc gtctcggcga
    15181 gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc gatggcaaga
    15241 cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac attcctgcag
    15301 ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa agaagttgtg
    15361 aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac catttatttg
    15421 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
    15481 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
    15541 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
    15601 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
    15661 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
    15721 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
    15781 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
    15841 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
    15901 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
    15961 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
    16021 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
    16081 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
    16141 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
    16201 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
    16261 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
    16321 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
    16381 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
    16441 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
    16501 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
    16561 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
    16621 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
    16681 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
    16741 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
    16801 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
    16861 agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt ttcttgcttc
    16921 aattagcata aatacctcct ttttattctg tttttgtata tagttagaca aaaactgtga
    16981 gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt tttatataac
    17041 gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg gtattttgtc
    17101 aagatattct tgtttttaag gcggaaaaat tgatttttaa tggatataac taaatatgtt
    17161 ttgataatta tcaataatat ttttagaatt atttatgtta tttatttatg ggtagatgaa
    17221 aagagacgga ggtaagaatg atggggagag taacaaatta tagtaaagaa tatttgctgt
    17281 tcaaaagtat ggtatacgtg gaagaatatg gaatgaagtc aattactgca agagagttag
    17341 cagagttctg cggttgttct acatacccga tttatacaca tttcaaatca atcagcgggt
    17401 taaaagagaa gatattagaa gaaatcactg tttattttga aaactactta gctgaatgca
    17461 gttccgatga tgttttaagc attgtctact tattgaaaga ttttttttct atccatgaaa
    17521 gtattcgtga aatcattgca aaatctgatt tagacatgga ttcgctattt aagcggccat
    17581 ttttctcata tgtcaaaggt cacacgaaga tcaaagaaga aaagtcgctt gaattggtat
    17641 ggcttaatgc attgggaagt ttgcgctcag atgattcttc tcaagatttt cttgaaatga
    17701 tgaacgctgt ttgggataaa aacgaggtga cggatgaata cttggaaaat atcttaaagg
    17761 aacagtcacc ttaggcagca acatgaaaaa gtagatcagc cacctttatt tttcgtaaaa
    17821 aagattgtaa aatgtctaac gaaacattgg aatgaaggag gaagcgacaa cgttaccaca
    17881 gcaatttatc aaaaaatatc gtcttctttt aggcgaagaa gcatccgatt ttttttctgc
    17941 gcttgaacaa ggaagcgtga aaaaaggatt tcggtggaat ccactaaaac cggcaggatt
    18001 ggacatggtc caaacttacc atagcgaaga attacaaccc gctccctaca gcaatgaggg
    18061 atttcttggc actgtcaatg gaaagtcttt cctccatcaa gctgggtatg aatatagcca
    18121 agaaccaagt gcaatgatcg ttggaacagc tgctgcagca aaacccggtg aaaaagtttt
    18181 ggatttgtgt gcggctcccg gtggtaaatc aacccaatta gctgctcaaa tgaaggggaa
    18241 agggttatta gtgacgaatg agatctttcc gaaacgtgcg aagattttat cagaaaatat
    18301 agaacgatgg ggagtttcga acgcaatagt gacaaatcat gcacccgcag aacttgtccc
    18361 tcatttttcg ggcttttttg atcggatcgt ggttgatgct ccttgttcag gagaaggaat
    18421 gttccgaaaa gatccaaatg caataaaaga gtggacagaa gaatctccgc tatactgtca
    18481 aaaacgtcag caagagattt tgtcttcagc aataaaaatg ctaaaaaata aaggacaact
    18541 gatttattcg acttgtacgt ttgcaccaga agaaaatgaa gagatcatta gctggcttgt
    18601 agaaaattat ccagtaacga ttgaggagat tcctttgact caatccgtta gttcaggacg
    18661 ttctgaatgg ggaagcgtag ctggtttgga aaaaacaata cgtatatggc ctcataaaga
    18721 tcagggtgaa ggacattttg ttgcaaagct gacttttcat ggacaaaatc agatgcataa
    18781 agaaaagaag acacgaaaaa aaagtaaagt gcaaatgaca aaagagcagg aaaaactctg
    18841 gacggagttt tcaaatgatt ttcattatga agctactgga agacttttag tgttcaacga
    18901 ccatctttgg gaagttcctg agttagcccc ttctctcgat ggtttaaaag tcgtacgtac
    18961 aggtttgcat ttaggagatt tcaaaaagaa tcgatttgaa cctagctatg cattagcgct
    19021 tgctacaaaa aagatagaga atataccttg cttgccgatc actcaaaagg aatggcaatc
    19081 ttacacagca ggagaaacat ttcaacgtga tgggaatcaa ggatgggttt tgctagtatt
    19141 agataagata cctgtcggat ttgggaaaca agtaaaagga acagtaaaga actttttccc
    19201 taaaggattg agatttcact gagtggatta gttcgtttat ttttttgtct atgctatgat
    19261 ggagatagat aaaaaggagc tgatacttat gaaaacatca acaaaagtaa caatcggatt
    19321 aagcgtagca gccgctgcca gtgtagcgac agcagtcgtt gtatcgggaa aagtaatgga
    19381 aaagatccat catatgacta ctcggacgaa agtaaaaaaa ttcgtctatg acaaatttga
    19441 tggaaatgaa aaattactag aaatcgtaga tggtctttca gatagtgact tggattcttt
    19501 gatgggaatg ttagcaaaaa tcaaatcagg aaaaaagaaa atctctgttt atggtgattc
    19561 aatcaaagat tcaaccgaag atgtaaaaga ccggctaatg cattttgttg aaaaaatgat
    19621 gtaagagaaa ctagcttttg aacactattc attcgaaata aggggttgtg acaaaattat
    19681 tgtcacaacc cctttaatct atctttaatt atgttcatga agcaataaaa gatagcaaaa
    19741 cagtaaatcg ttggcttttt ttagattgaa accagactgt tcttgaaatt tttcgattcg
    19801 atattttaga gtattacgat gaagaaacag tgtttttgca gcagagctga cattgcctaa
    19861 ttcattccat aattcttgaa tgatcacttc catttcattt tgtttgatca gttctatgta
    19921 acttttgatc aacttactat cttttacagt atcttttgta tagtaatgta aagccacatc
    19981 ttgcatagaa aaagtacggc tgtgagtata aagattgttt tgctctgtta aaaaaatttt
    20041 tctttcttca gcaaacaaag ggacgaggtc ggtgccaggt gaatggaaag aaccaacgaa
    20101 tacagacgtt gtcgtatcaa aatcagcatc taaagataga aaaataccgt tgatttcagt
    20161 agcatcaaga taatcacggt ttatttgttc gactaagagc gcatctgtgt ctgtataaaa
    20221 aaagaaatca gcagaatagc gaaataattc caaaatagta tcttgccatt ctttttttaa
    20281 gaattctcct tttgattcga cgtgaagctg gatcaaacga aagctttttt cttgaacaca
    20341 agaagcatct ttaaaaagat agtgatacca tgggtgtttt ttttgatcgt tgattacagg
    20401 gaataacgct ttcaaaaggc tggtttcttg ttgagataat gaatcttgtt tgatccagat
    20461 aaaagcatca tcaattgcaa aagaaaggat atccgcttct gatgctggag agtttttttt
    20521 ctgtgcttgt gggtaaagag caagtaattt atccattttc atcttgttca cctcttgtat
    20581 ttattttacc atagaaaaaa aagttagtta cagccttttt atcatatgct aaagtaaaac
    20641 aagaaaaaac aaaggcaggg aagggtaatt ggtaaataca aatcttgatg cagtagaaaa
    20701 agaaaaatgg atgcgccttg cattggcaga agcaaagaaa gcagaaatgc ttcatgaagt
    20761 tccgattgga gcagtggttg tattggatgg aaaagtgatt ggaagaggat ataaccttag
    20821 agaaaccacg caagatgcta ctacacatgc agaaatgctt gcaatcaagg aagcctgtga
    20881 aaaagtggga agctggcgct tggaagatgc tgcacttttt gttaccttag agccttgtcc
    20941 tatgtgcagc ggcgcgatga ttttgtctcg tgtagcagaa gtttatttcg gcgcgtatga
    21001 tccaaaaggc ggaacagccg gtaccttgat gaatcttttg gaggacgagc ggtttaacca
    21061 ccaagcatat gtagaaggtg gcattttaga agacgaatgt gggatgattt tgactgactt
    21121 tttccgaaag ctccgccagc gaaaaaagaa attaaaaaaa gactggtcaa cgaatgaaaa
    21181 ataaggtata ctattctttg ccgaaaggct aggacaatgg tgggcttgtg aagcgtgtca
    21241 ggtctggaag gaagcagcac taagcaggtt ccgccatgtg tctgattata atgaaacgac
    21301 aacagacttt cgggtctgtt ttttttattt ttatagtact gtaaaaatga atgttcctga
    21361 ttttttagcc agactgattt atcgaatatt gtacttatac aatttgcttc aaggcaggaa
    21421 aaactctgaa atagagcttg tttcaatgga agaaaaagct gcctatggta tgataaagat
    21481 aaagagaggc gagagggtac atggaaaaaa tagtgatttt acatacgaat gatctgcatt
    21541 ctcatttaga aaattggcca aggatcagaa ggtttttgga tcaacgtaaa cgagaaaacg
    21601 aaaagaaaga aaaaacgaca acgattgcag tagatctagg tgattttgtg gatcgttggc
    21661 atccattgtc agaagcaacg aatggacagg aaaatatctt gctgatgaat gaagttggtt
    21721 atgatgcagc gacgatagga aacaatgaag gcgtaggaaa ttccaaagaa gagttgaatc
    21781 atttatacga tcatgctgag tttgatatca tcctggataa cttattcgat aaaaatacgc
    21841 tccagccacc ggcatggacg aaaccatata aaatcattac aacgaaacac cagacgaaaa
    21901 tcggactaat tgcatttact gctccatttc cgcttactta taatccgaat ggctgggaca
    21961 tacggaatcc ttatgatatt ttgccagaat tagtcgaaga tttacgttca caagtggatg
    22021 ttttagtttt gatgagtcat ctcgggattc aggatgattt ccaaattgca aaagaaatac
    22081 cagcaattga tgttatttta ggatcgcaca cccatcattt attcagagaa ggtcacgtaa
    22141 tcaatgatgt ccaaatagca gccgctggaa agtacgggca gtatgtcggt gaagtccatt
    22201 taaccatcga tgaagagaaa aaaatcctta agcattcagc caaagccatc cctaccgaaa
    22261 caatgacagc ttttacagaa gatgaacaag aaataaatag ttatctagaa cgtgggcatg
    22321 aattattaaa agaaaaagaa gtagcagatt tgccattttc tctgagtcta gatatcttca
    22381 atgagcattc gtttatccaa gtagcctttg aggcggtgaa agaacgagga aagacggaag
    22441 cagcaatatt aaacagtggt ctgtttttaa ctgatattcc aaaagggcgg gtcaatcagg
    22501 atcagcttca cactgcgttg ccgcatccga tgcacttgtt aaatgtcaca ttgggtggaa
    22561 atgatctgat ccgcttagtt ttagaaatag aaaaaaatcg taacttttta cgtaactatc
    22621 ctatgaaggg aatgggattt cgcggtaaga tttttgggca gatggtctat aatggaatca
    22681 catatgattc tattaaccat caagtctttt ggaagaacaa gccaatcgat ctgaataaaa
    22741 attatacttt tactaccgta gatcatttga tgtttgttcc attttttcca acgattgaga
    22801 ttgctggaaa aaatgaattt ttgttccctg aatttatccg aagtgtggta ggagattact
    22861 tgaaagctca ctacccaatc aaataaaaac aaggtataat aaaaaagata ggtggtgaac
    22921 gcatgtctgc aaaacaaaat gaacagcaga acgttgagca taaaacagaa attgctaaga
    22981 aaaaatcatc agctgaacag gaaacaacag tcaccgaaga actgacagct gctttggaag
    23041 aaatcgttga ggagcctaaa gcagaaaaag tcaaaggcga gctagtgaca ctgttagatg
    23101 acagtaattt tttgataggt gaaagacatt atcggcttgt gtccgattat cgtgaaggat
    23161 ttaatgcaga aaagcttgga gaacgttaca gtgatgtact agcccgatat gattatatcg
    23221 taggagattg gggttatgaa caactacgcc taaaaggatt tttccaagcg gataatcgac
    23281 gggcgcatcc agatcagcga atcgattcct tggaagacta tctttacgaa tattgcaatt
    23341 ttggttgtgc gtattttgtc atcgagagaa tcggcggtaa aaaagaaaag acaactcagc
    23401 gccgaaaaaa gaaaaaaaac cataatcgtg cgtatatcga tgagaaaaaa ggaccagtcg
    23461 catctaatcg caaaaaaccg gttattaaaa atcgaaaaac agagtcagca aaaacctctg
    23521 aaaaagtaaa aaaacaagaa caacctaaac aagtagaaaa aacaaaaaca gcatttacga
    23581 tcagaaaaag ggaagagtag tggatgaatg gactataaag gatatttaat tgatttagac
    23641 ggaacgattt atcgtgggac acaacccata ccggccggta aacgatttgt ggaagaattg
    23701 caaaaaagaa aattaccttt cttgtttgtt accaataaca cgactaagtc accagaaaca
    23761 gtagctaatc gtctagcaga tgagtttgac attcatgtag ctccagagac agtctacaca
    23821 gctactttgg caacaatcga ttttatgaaa gcagatggaa aagggaaaaa agtctatgtt
    23881 atcggagaag caggattgat tgacttgatt ttagctgccg gatttacatg ggaagaagag
    23941 gctcctgact atgtagtggt aggcttagat aattatctga cctatgaaaa agtagttaaa
    24001 gcaacacttg ctattcaaaa aggtgctact ttcattggaa cgaacccaga taagaatata
    24061 cctactgaac gcggccttct tcctggagca gggtcagtca tctcttttgt agagacagca
    24121 acccaaacgc cgccgatcta catcggaaaa ccagaagcca tcattatgga taaagccgtt
    24181 gaagtattag ggctgcaaaa agaagaagta atcatggttg gagataacta tgaaacagat
    24241 atccaagctg ggatacgcaa taatatagat actttgctcg ttttatcagg ttttacaaaa
    24301 gaagaagatg tacctgggtt gcctatccca gctacctatg tgaaacaatc actggatgaa
    24361 tggagcttct aatatgaaaa attcgagatg gcagtggatg gaatatgctg gattgttcag
    24421 cctatttctg acactgatca gtctggcagt tggtgtgacg atcaactttc gaccactata
    24481 cgtatttgat atcggacatt tgcaaatttt ggattatact tctttagatc aagagacgtt
    24541 actgaaaaat tttgatcacc taatgaatta tctcaataat cccttcaaaa ccatcctcag
    24601 cttgccagat ttcccagttt ctgctagcgg agcccatcac ttttatgaag tgaaaatttt
    24661 gtttttagtg gattatgctg tgtttttcat cactttgatc ccgagtattt tgtttatcaa
    24721 atatttgcaa aagaatgatc ggctttggcg gttgattcgt ccgtttcaaa tcggcatgtt
    24781 actgccagtt gttttcggtt ttttcatgat gataggattt gatcgctttt ttatcctttt
    24841 tcatgaaacg tttttcaaca acgatgactg gttattcgat cccgttactg atccaatcat
    24901 caatgtactt ccagaagaat ttttcatgca tagctttatc ttattttttg tattattaga
    24961 attatttttt gctgtttttc ttttcttagg aaaaaatagc ttgaaacaaa
//
LOCUS       DACCDF010000038        24826 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCDF010000038 DACCDF010000000
VERSION     DACCDF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.038"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..89)
                     /locus_tag="IVK51_001533"
     CDS             complement(<1..89)
                     /locus_tag="IVK51_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6431133.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKG"
     gene            397..1203
                     /locus_tag="IVK51_001534"
     CDS             397..1203
                     /locus_tag="IVK51_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431134.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1215..2435
                     /locus_tag="IVK51_001535"
     CDS             1215..2435
                     /locus_tag="IVK51_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431135.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2425..4011
                     /locus_tag="IVK51_001536"
     CDS             2425..4011
                     /locus_tag="IVK51_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6431136.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            4050..6188
                     /locus_tag="IVK51_001537"
     CDS             4050..6188
                     /locus_tag="IVK51_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6431137.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6476..7867
                     /locus_tag="IVK51_001538"
     CDS             6476..7867
                     /locus_tag="IVK51_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP6431138.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7926..8735
                     /locus_tag="IVK51_001539"
     CDS             7926..8735
                     /locus_tag="IVK51_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6431139.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8707..9696
                     /locus_tag="IVK51_001540"
     CDS             8707..9696
                     /locus_tag="IVK51_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6431140.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            9810..10805
                     /locus_tag="IVK51_001541"
     CDS             9810..10805
                     /locus_tag="IVK51_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6431141.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            10809..11951
                     /locus_tag="IVK51_001542"
     CDS             10809..11951
                     /locus_tag="IVK51_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP6431142.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            11948..13375
                     /locus_tag="IVK51_001543"
     CDS             11948..13375
                     /locus_tag="IVK51_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP6431143.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(13442..14731)
                     /locus_tag="IVK51_001544"
                     /pseudo
     CDS             complement(13442..14731)
                     /locus_tag="IVK51_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(14911..15321)
                     /gene="tagD"
                     /locus_tag="IVK51_001545"
     CDS             complement(14911..15321)
                     /gene="tagD"
                     /locus_tag="IVK51_001545"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP6431144.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            15447..16838
                     /locus_tag="IVK51_001546"
     CDS             15447..16838
                     /locus_tag="IVK51_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP6431145.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            16971..17918
                     /locus_tag="IVK51_001547"
     CDS             16971..17918
                     /locus_tag="IVK51_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP6431146.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(17958..19118)
                     /locus_tag="IVK51_001548"
     CDS             complement(17958..19118)
                     /locus_tag="IVK51_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6431147.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            19283..20458
                     /gene="mltG"
                     /locus_tag="IVK51_001549"
     CDS             19283..20458
                     /gene="mltG"
                     /locus_tag="IVK51_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP6431148.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            20594..21073
                     /gene="greA"
                     /locus_tag="IVK51_001550"
     CDS             20594..21073
                     /gene="greA"
                     /locus_tag="IVK51_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP6431149.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            21241..21972
                     /locus_tag="IVK51_001551"
     CDS             21241..21972
                     /locus_tag="IVK51_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP6431150.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            21969..23036
                     /locus_tag="IVK51_001552"
     CDS             21969..23036
                     /locus_tag="IVK51_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6431151.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            23043..23675
                     /locus_tag="IVK51_001553"
     CDS             23043..23675
                     /locus_tag="IVK51_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431152.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            23815..24480
                     /locus_tag="IVK51_001554"
     CDS             23815..24480
                     /locus_tag="IVK51_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP6431153.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            24508..24801
                     /locus_tag="IVK51_001555"
     CDS             24508..24801
                     /locus_tag="IVK51_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP6431154.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8462 a         3689 c         4805 g         7870 t
ORIGIN      
        1 cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat
       61 ccgagtaatt ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg
      121 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt taaacgaaga aaaagtgtta
      181 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaacccc ttttttggtt
      241 tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata
      301 gaaaattaag aaagaaataa gaacgcagct cggtaccaaa aatatggtaa aatggcactt
      361 gactgtaatt cactgaaatc gcttggagga acacaaatgt ttaaagattt gtatttattt
      421 atgaaagaca tttatcagaa tagaaaatta ctgctacagt tttcgatcaa tgacttcaaa
      481 tcaagatatg caggatcgtt tttaggaatc ttatgggcgt ttatcaatcc gctatttact
      541 gtattgattt attggctggt atttggtgtt ggactgaaag cacaaatgat cgatgcaaaa
      601 tacccattta tcgtatattt agttaccggg atgattccct ggttcttctt ctcagatgca
      661 tttgcatcaa ctacggttgt ttttcgggaa tatacttatt tagtaaagaa agtcgtattc
      721 aatattcgta tcttgcccac gacgaaaatc ctatccaatc tgtacaccca tttattcttc
      781 attctgattg gcatagctgt cgcgcttata aatggtatat atccttcagc aatgtcttta
      841 cagctgattt attatttatt ttgcatgtgt gctttcttga caggattgac ttggctgacg
      901 gcatcgatcc aaccattttt acctgatatc atgcaattaa ttacgatcgt gctgcagttg
      961 atcatgtgga cattaccgat attatggagc cctaatcagt ttgattcagg tcttgtaagc
     1021 atattgaaat taaatccgct gtactatgtt gttcaaggtt atcgtgagtc atttttaagt
     1081 gaagcttggt tctgggagca tggaacatat actttgtatt tctgggtttt cacgttagta
     1141 atgttcgtaa tcggctctgc agtgttccgt agattgaaac cacatttttc agatgtgctg
     1201 taaggagaat agctatgaca gaatatgcaa ttagactaaa agatatcaca aaatcttata
     1261 atatgtacgc aaaaccttcg gatcgatttc gtgaagccct caatcctttt aagaaatcct
     1321 atcatgatgt attttatgcg ttgaaaaacg tgaatgtaga aataaaaaaa ggtgagatga
     1381 tcggtttcgt gggagaaaac ggatcgggaa aatctacaat gttgaaaatc attacgggag
     1441 tactgacacc aacatccggc acgatggaaa tcgaaggaaa aatctcagct ttgctagaac
     1501 taggatcagg attcaatcct gagtattctg gctatgaaaa tatctattta aatggaatgg
     1561 ttctaggatt ttcaagagaa gaagtcgatg aaatggtcga tgatatcatt gaatttgctg
     1621 atattggcga ccatatttat caacctgtaa aaacctattc aagtgggatg ttcgttcgtc
     1681 tagcttttgc tgtagcgatc aatgtgaatc ctgatatttt gattgtggat gaagcattgg
     1741 cagtaggaga tcttgaattc caactgaaat gtatggaaaa atttacagaa attaagaact
     1801 cgggaaaaac gattttattc gtttctcatg atattagttc gattcgccgt ttttgtgatc
     1861 gttcctattg gttgaaaaat ggagaagttg tcgaatatgg tgatacgatg gaagtaacga
     1921 cgaattacga aaactttttg aagaaaaaat cagttaaaac agtagatcga aataaaaacc
     1981 aagtagaacg cttcgcagct ccagatatcg tagacgtgac gaaagccgaa ttgttggata
     2041 aaaataaaaa tccagtggat atgctggaac aaggagaaaa actatacgtt aaagtaacct
     2101 atgaagtaaa agacgataca atcaaaaatc cagttttagg aattgctctg cgtacagtcg
     2161 acaattttta tgtgtgcgga ctcaatactc tactagatgg tatcaagatc ccttggaaaa
     2221 aaggtgagaa tatcttttat ctggagtatg aaaagttagg tttgttagct ggtgaatatt
     2281 attttgacgt tgcggtcttt gaagaaaatg caacggttcc tttggtatac aagacaaaat
     2341 acatgaactt gtttgtcagc ggttcttata tcggagaagg catcgtggtc ttagaccata
     2401 aatgggaaga agggactcat agcaatgaaa tatgattttg aaatggacct agatgaacaa
     2461 agcagtgtgg gaaaaattgc cgcacagatc aaaccaggaa gtaaagtttt agagtttggt
     2521 cccggaaacg gtcgattaac gaaacatttg atcggagcaa aacaatgtga agtgagcatt
     2581 gttgagctag ataaggaatt attcgatttt gtcagcgaat ttgcccaaga tggattttat
     2641 ggagatatcg aaagttttga atgggcaaac tattatgctg gacagacatt tgattacgtc
     2701 ttgtttgcgg atgttttaga acatttagta gatcctggaa aaacattgaa gaaagtcaga
     2761 gaatttttga atgaagaagg agagatcctg attacattcc ctaacttagc acataattct
     2821 gtcatgatcg atttgttcaa taatcaattg ccttgggctt cttatggtct cctagatgaa
     2881 acccataatt ctttttatac tcatgatggt ttccaaaaag tttttgaaaa agccggatta
     2941 ttcattaata ttgaagatta cttgtacttg gctgtaggag atacagaatt aaaatcaact
     3001 tatgaagagt tgccggaagc tgtacgctat gatttcaaaa tgcgaccatt tggtgaagtc
     3061 taccaatact ttttctcatt gatgaaacat ccagtagtac aaacttccat tgcacagcca
     3121 caaaactcta attatgtaaa agtactagaa gtaacccagc aaacgaaaca agatgaaaca
     3181 atccaacaaa taccattcaa taatttcaca ggggaaaatg agacactaag tttccccgtg
     3241 tcagaagcaa cagaaagaat ggtgtttcgc ttcgcacagc aacctagctt tatcgaattc
     3301 agtgcagaag ctgctggaga aaaactcata tttatcgatt caaatgctgt agtcaaaaca
     3361 gtaaatgatt gttatttgtt tgatgggaaa gaaataccag agttcgctct aacggatatt
     3421 tctgggaaag aagtgactat ccattgtcac tatcgcttta tcggggaatt gacaccaaca
     3481 atgaaagaat tgctggaaac aattcgtcct atggctgaag tcacaaagca gctttctgag
     3541 aaaaatgatg aattagaaag agaaaatcag catcttcttg aggaaaatac aagactagac
     3601 catacgttaa aaacaaccac aaatcgctat tgcacattgt tggaaagtga tgaatggacg
     3661 atcaaacata gactaggacg acgtaagaaa aaaacatcga aaaaaataca agaaaaagag
     3721 ctttctatat gtattgatga gaaaatatgg gatgcagaaa caaaaatatt aaaaatcatc
     3781 ggatggggaa tcgctaattc ggatcgccag ccattgtcct ataagttatc agctgaccaa
     3841 tcgccatttt tcgaagcaat tcctgtttca cgagatgaag tcaatcaagc agaacagctc
     3901 gcaaaaggaa cagaagcggg atttgaatta cgaatccttt gcgagcaaga acgttcattc
     3961 ttagtggaag cagtagctca aaatggtcaa tcatggatca ttgaaatgta aataatgatt
     4021 aacttataaa gataaggagt aaacgaatcg tgcaaaaaaa agaaattgcc gtatttatcg
     4081 atcaaatcac tcgtgaacgg gccactggcg atttgcttat tgtgggctgg gcaattgatg
     4141 aagtgaccaa agaaatccct acgataaagg tagaaaaaga aaatgtgatt gctgaagcaa
     4201 cacatgtcgt aagattggat atcaatcatt tatataatct tgatgttaag acccaaagcg
     4261 gttttaagat acgtttatcc gggaaaatga gaggaaaagc gatcttagat tttcaaacgg
     4321 caaaacatca aaatggcata gctgtgaaac taaatggtaa atacccttat gatgacggca
     4381 ttgaatcttc atgggaaaga aaaaaaagat tactgaaaaa agggataaat tatgcaagga
     4441 ctcatggcgt aaaaaaaaca atccgccggg tcaaactgga attgaaacca ggatcgatcg
     4501 actatgcaga gtggatcagt cgtcacgaga aacctgatct gaaagagcaa aggaaagaag
     4561 cacaagggtt tgcataccgt ccattgattt ccatacttat gcctgtctat aacgttgaaa
     4621 tcaaatggct cgaaaaatgc attgattcgg tattggatca aacgtatgat cactgggaac
     4681 tatgtatcag tgatgatgct tctactgatc cagctatccg aaagtgtctg gaatcctatc
     4741 aagcaaagga tgatagaatc aaagttgttt tccgacaaga aaatggacat atcagtctgg
     4801 caactaattc tgcgttggaa atggcagaag gagaatttat agcgctctta gataatgatg
     4861 atgaattacc gccatttgca ttatatgaag tggcaaaagt gctaaacata catccagaac
     4921 tggatttgat ctacagtgac gaagataaaa tcgatgcaga tggcaatcgc tttgatccgc
     4981 acttcaaagc agactggtct cccgatacat tgatgggaaa taattatatc tctcacttag
     5041 gcgtatatcg aacaagtatt gtgaaaaagc ttggcggttt ccgtaaaggc tacgaaggct
     5101 ctcaagatta tgatttggtc cttcgtgtga cagaacaaat ccctgcagat catatttacc
     5161 atattgaccg agtcttgtat cattggcgga cgatcccagg atcaaccgca agtaatggtg
     5221 aagccaaaag ttatatctat gattctggag tgaaagcatt gacagatgcc ctgtctagaa
     5281 ggaatatcaa aggaagcgta cgtcctggca gaatttctgg attttatgag attgcttatg
     5341 acgtcttgca agaggatttg gtcagcgtga tcattccaac gaagaatggc tatgaagatc
     5401 taaaaacttg tgtggattcg attatcgaaa aaacgtctta tccaaattat gaaatcatta
     5461 ttgcagataa tggaagtaca gatcctaaaa tgcaggaatt atttgcagaa tacaagcatc
     5521 agctgaaaga ccgatttatc gttgaattaa ttgacattcc attcaattat tcacgtatca
     5581 acaatctggc agcagaaaaa gcaaacggca aatacttttt attcttgaat aacgatacag
     5641 aagtgattga acctgactgg atgacagcga tggtttctta tgcacagttt gatcgaatcg
     5701 gctgtgtagg agcaaaactg ttttatccag atgatacaac gcagcatgca ggagtattat
     5761 taggaatcgg tggtgttgca ggacatgcat tgaataacta tgatcgaaca cattgtggtt
     5821 atttcggccg attggtcatt gacgtcaatt acttagcagt cactgcagct tgtatgatgg
     5881 tcaaggcagc tgacttcaat gctgtcaatg gatttgatga aacattagaa gtagcattca
     5941 atgatgttga cttgtgtttg aaagtttatg aactgggtcg ttacaatgtc tatgcacatc
     6001 aagcagaatt gtatcatttc gaatctaaat caagaggtta tgaagataca ccagaaaaac
     6061 aaagacgttt tgccggggaa ataaaaaaaa tgcaagataa gtggccttct tatattgctc
     6121 atgacccatt ttataacgat aatctaacta aagaaggaat cggcgatttt tcattgagac
     6181 ctgattgatt aaaagaactg tattttcatt tttattgatg aaaatacagt ttttttagta
     6241 tatgaaaatt gtaacttgtg accgaagaga tgataggaag aacacccatt cagtaggaag
     6301 aatgtgatac gatttttgtc caactctctg ttaggcagaa atatacatat aaacatacaa
     6361 tagtcacctt ttctagtttc ttttaattta ttttacaatt tggttattca ctaggttttt
     6421 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
     6481 taaaaatggg gagtggaatg cggcacgtag gatattcatt atcatcatgg atgtacttgt
     6541 attgaatgtg tcgatctatc ttagcttttt gctgaagttc caaggagaag tcccagcaag
     6601 aaacttagag acctttcagc attcagcagt ttttatttcc attatcttta ttgcgttgaa
     6661 tattttgtta ggagcttatg tattctatag ccgagtgatc agtgatatcg tcttcattac
     6721 tgttattgga caggtgctga tagcattggg gatcatggta gttacttttg ctggacgttg
     6781 gtttgcattt ccgcgtggcg tcatcgtgct ctcgtttata cttggcacga tattacttag
     6841 cgtttatcgg atagttgttt ataaactata cttaagagtc agtcgtgata agaaagtcct
     6901 tatcgtaggt ttggagaaag atgtcgcacc agctatccaa aactttcaat ctaagaaaaa
     6961 caacaaacat aaagttgaag cagtagtgat cgacaagtat tacgaaaaca caaaaaaatt
     7021 gattgatgat atcgatattg tctacttggc ttctaaaatc ccagaagacg aaaaaatcaa
     7081 tatttatcaa ttagtaacta aaaaagagaa gaagctattt ttaaatacaa cttttgaaaa
     7141 tttaacgatg atcaatccga acatcatgaa tttcgaagat gaaagtatca ttgaagcttc
     7201 cggtttcaaa attcagccgg aatatgaatt attcaaacgt ctttttgata tcatcgtttc
     7261 gcttatttta ctgattcttg cttcaccttt tatgctaatc acagcgattt tggtaaaaac
     7321 aacttcacct ggaccaatta tttataaaca agtacgtatt acgaaaaatc aaaaggaatt
     7381 ttcaatctat aaatttcgtt ccatgagtgt aacagctgaa gcaaaatcag gaccggtctt
     7441 ggcaaagagc aatgatgcac gtgtgacacc agtaggcaaa tttatccgtg ccgtacgttt
     7501 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgatcg tcggaccacg
     7561 tccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attatttaag
     7621 acacaatgta cgtgcaggga ttaccggata tgcacaagta tacggaaaat atgtatcaga
     7681 ttataatagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct tactattaga
     7741 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct cacaaggact
     7801 agatgaagat gaaaatatcc gttcgttagc agaaatcgac ttttcacaag agaaaattta
     7861 tcgctagatg ggaaaaattt tgagcaaaga agtaggagag aacattggaa aataaaaata
     7921 taagtatgct gctttcttta tatgataaag aaaaaccaga gtatttaaag gaagcattaa
     7981 ctagtatttt tgagcaaaca gtaatgccaa aagaaattgt attagtttat gacggcccta
     8041 taaatgaaaa acttcaagta gttgtcactt atttccaaca aaaagatcct cacttgttta
     8101 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaatt ggattagaac
     8161 atacgaaaaa tcgcttagta gccagaatgg atacagatga tatcatggaa ccccaacgat
     8221 tagaaaaaca attagagatc ttccaaaaag atccagaaat agcgattgtt gggagtaata
     8281 ttgaggaatt tactggcacg cttagcaatg ttatcggaag aaggattgta cctgaatata
     8341 atgatgaaat ccgtgtgttt tcgaaaagaa gaaatccctt taatcatatg acagtcatgt
     8401 ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga tttgaagatt
     8461 attatttgtg ggtaagactg ttgaaggcgg gatataaagg gtataatatc caagagattc
     8521 tagtccatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt aaatatcttt
     8581 taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt
     8641 ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa attaggggaa
     8701 aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat acctttttac
     8761 aatgcaaaaa atactttaaa aagagctgta gacagcgtct taacgcaagg ttatcccaat
     8821 attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat tggtgacgaa
     8881 attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg tccatcagtt
     8941 gctaggaatt gtgggataaa agaagcaact ggaaatttgg taggattttt agatagtgac
     9001 gattattttt tacctgattg ttttaataat gttttgaaag actaccatag gaattacccc
     9061 gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt
     9121 cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag
     9181 gcagtggatt tttacgtatg gaataaattt tttaaaaggg aattattcaa tcatatagtt
     9241 tttccagaaa ataaattata tgaggatatg gttcctatgt atgaagcttt tagagcggct
     9301 agtaaaattg atattttgcc attttcaggt attttttatt atcaaaataa tgatagcatc
     9361 gtttatcagt cttttaattc aaaacagtat gataatattt tgcaaagaaa aatattagtt
     9421 gatgaagtaa agaaagatta tcctcgactt attaatttgg ccaatgctcg attagtagac
     9481 ggataccttt caacagggtt taaaattact ggcaagaatc aaaatcagaa agaaaagcaa
     9541 aaatattata atcagtctaa aagagaaata aaaagtatat attcaaaaat aagaagtaac
     9601 aatgagatat caattgctaa aaaattagct ttatttttat acttaattaa tcctaaaact
     9661 tattttattt tatataaaaa agttctaaaa aaataaagtt tttttcgata tgttattttt
     9721 tatgtttatt aacttcggaa gactaaaatt gttactggta atgatacatt tgtaaaaata
     9781 aaaaagatga tttttaggaa gtgaagagag tgcataaaat tagtgttata gtacccgcat
     9841 ataacgtaga agactattta gaaaactgct tagaatctat ttccaaccag acacattcaa
     9901 atattgaaat aatagtagtc aatgatggtt ccccggatcg aagtggggaa attgcagaag
     9961 aatataaaaa gaaagatatg cgattaaaag tgatacatca agtgaatggt ggtttaagtg
    10021 cggctaggaa ttctggtctt gattctgcaa caggagattt tgtgtgcttt attgacagcg
    10081 atgattggat agaaccagac tatcttgagt tactatattt tggcacgcaa acttatggag
    10141 cagatatctc ggtgctaaaa ataaaaaaga tttcaagttt agaacaacta aaagaacata
    10201 caattgctga gagaaaatgg gaaatatttg atagagaaca agcaatgaaa aacttattta
    10261 taaataataa aattggctat tctgcaaata ataaattatt caatagaaat ttatttgata
    10321 atataagatt cccactgggt aaattaatgg aagataaggc tactacttat ttgctgatcg
    10381 atcattccga aaaagttgct gtcaattcat ctgctaaata tcactattac caaagcccta
    10441 aaagtatact gagaggaagc ttcaatccta gaatttttga ttcttttgaa gtccatgaag
    10501 atatactaat attcatagat aagttttatc cggaattatc aattaatgtt agaactcgct
    10561 atgtctatga agcaataagg atgatgatga caatgattaa aaataactat accaataaag
    10621 aagattattt acgttctata aatattttac aacgctttga aaaagaagtg aaaattgatc
    10681 ccgacttttc accaaaatta aaaaaattag taaagttttt attaataaat aaaaacttgc
    10741 cttactatat gtcaagtagt aagatagcaa gtaaattctt gattaagata gaatctgtga
    10801 ggtaagagat gaagaatata aaaaaatttt tttcaaggaa tattttcccg gtttttatta
    10861 aaatattacc tattcaaaaa aatttaattg tattttcaag ttttggcggg cgtaactatt
    10921 ctggaaatcc aagagttatt tcagagaaaa ttcatgaaaa aaaattgaat tacagaatta
    10981 tttggttaat caaaaaagaa gcgattataa ctgatttacc agattatatt aaaagaattg
    11041 actccgattc ttttttagct gccttttatt tatatacagc taagtattgg atagacgata
    11101 gtcggaaaac gattaagtat gcaaaaagga aaaaacaact ttattttcaa acatggcacg
    11161 gaacgccttt aaaaaaaatt gaatttgatg caaaagataa gttacctaaa cgttacctaa
    11221 gctatgcgaa aaaagatagt gagtcaatta catatttatt atctggtaat cgctatagtt
    11281 ctaagaagta tgtttcagca ttcaatatcg ttccttctaa aatattagaa gtaggaactc
    11341 ctagaaacga tgaattagcc tctagtaaag agcaagtgtt tgacttgaaa aagaaacaaa
    11401 tcaatgtctt atttgctcca acttttcgaa ataatattga ggacaacgga attacgcaac
    11461 tagaatggtt aggtgtggat aacttacgtg aattttttaa aaagcaacaa caagaattaa
    11521 attttatgac taaatttcat cctaatgttc atagtaaatt agcaactgat tctaaaagta
    11581 tagaatatat gaaaaaacat caaattactt taattaatga tgggattcct cttgaaatgc
    11641 tttttgaaga gatagatgtt ttaatcacag attattcatc tatttttttc gattttgcgc
    11701 taacaaaaaa accaattatt ctttttaact atgatgaaaa agaatatagc ttagaaagag
    11761 gtttttatat acagttaagt gagttaccaa ttataagcgt acaagatagt tatggccttg
    11821 ttgatgtttt taaaaaccaa caagaaaagc ttttgaaaag ttctcaagag ttacttgatt
    11881 atattggaaa ttatgaaaca ggagcaagta cagaaaaggt tcttgagttg atagagaggg
    11941 atataaagtg aaaaaaacag taaaagatat attttataat gcgatatatc aagtcttttt
    12001 gattgtttta cccttactta ctatacctat acttagtcgt agaattggtt caactggttt
    12061 agggatttat gggtatgttt tctcaatatc acagttttta atgactgtca ttgctgtggg
    12121 aatgaatcct tttcgtattc gaaatatcgc taaatcaagg aaagataaaa aagcactatc
    12181 tttacaattc tggaatattt actttattca atttttaata ggcttgagtg tttcctttct
    12241 ctatatagcc tatatttatt tttttgatgt aaagtataaa aatttatact taattcaatt
    12301 gatatttatt gtagggctaa cattagatat ttcttggttt tttcaaggaa ttgaagaatt
    12361 ttcgaaagtg gttattagaa atacagttat caaattatca tcggtattgt taataatcat
    12421 ttttgtttca actaaagatg atttgtggat ttacgtgttc attacttcaa ttactaactt
    12481 tttaggaagt ttagttttct ggttaagtat ccacggaaaa gtaggtaaac caatctttaa
    12541 ctctaatgtt tttaaacagt tgtggaaacc agcttttatt attttgactc ctcagttgtt
    12601 tatgcaagtg tacacaactt tagacaaaac gatagttggg tattttgtgg atccaacgga
    12661 attatcttat tatgatcagt ctcaaaaaat tgctcgtatt atattggcag tcttaacatc
    12721 aattactatt gttatgttgc ctaaaatatc taaagcacag gcagagggga aatcagaaaa
    12781 tattttagtt tacacaaaaa aatcttttga ttacacgatt atcattgcat taattctttt
    12841 tagtgtggtg tttatcaata caaaagaatt tgttccatgg ttttttgggg agaatttcaa
    12901 accgatgaca ataaacatgc ttatttcttg tctaattata gttgttagtc caattggtgg
    12961 agtttttgct aaccaatttg caattgctat agaaaaagat aaggagtatg cttatccaat
    13021 gatcattggt gcgattgtaa gtattattgg aaatgtagct tttgtgccta cttatggcgc
    13081 tgtagcggct acgatcgtac tcgtggtagt agaattatta gtctttttct ttagagtatt
    13141 ttttgtacga aaagaaataa aaatatccta tttctttggg aagaacgtta tcttattttt
    13201 tatagtcaat cttactttga ctacttgggc ctattacttt cttccacaat tattctcttc
    13261 ttcattttta gatatggcaa ttaaatcaat aatgattttc attatttatc ttccgtttgt
    13321 ttattttctt tttccagata tacgaaatga aataagaaag attactcgaa aataaaataa
    13381 tgttgtaaaa aagtagttct ttgtcaaatc gggctgataa ggtcatttat gaacaacaaa
    13441 gttagtaaga gttactagct tttaatgcat agctgattaa cctgttgatt agaaaaatcc
    13501 acaagcgcat attggtaaaa ctgcgaaacc cataagcaaa tcgtttcaat gttttgatat
    13561 gtgtgcgaag attctctaat tttgttatta gaatactttt tcgaaaatgc tagttaaaag
    13621 cctttttcag atatgaaaaa ggctttttga gatagtgaag tttcgaggga acttctttgg
    13681 tcgtttctcg aagtagctag ctgtttcttg tgataattgc taagcagctt ttgataaact
    13741 gcttagcaat tttaagttca taagagattg agggtagacg atcaactact tcactttctg
    13801 ttaaatagcg ccattgaaaa ttctgccatt tcttgaatgt tgtgtaatcc aactcgtcat
    13861 ctgattttag aagtaattta tacaaaggtt tcaattgtcg atagtactta gcttgtgaag
    13921 tgtctttttg attcaactgc ttcatcacac gttttcgaac acgatcgaaa aacttgttca
    13981 agtgttgtcc aatgtgaaag caatcaatga tgatttgggc gtttggaaag gactctttga
    14041 ctaatgaaaa ataaggggca ttcatatttg tgacaatgta tcgaaccccc atatgagctt
    14101 ttcttgtaaa acgcatgaaa taagcaatca aatctttttt tctccggctg gacaaaatgt
    14161 caaagagctt ccctgtctct ctattcatac aagaaaagga gaactttcca tgggaaattg
    14221 aaagagagcg aaactcgtcg aatagtaaac aatgaggtaa atagtctagc atctgtcgca
    14281 gcattgccga tagagcagat aatgtccgtt gaaatgtcgt tactgaaaga tcacaacgtt
    14341 tcgtaagtgt ctttaacgct tgacgctcgg tcaactcttc taatatcgtg taatgaactt
    14401 gtttcgaaat aaaacatgaa ggaacgacaa gatacgtatg agcagtaaaa tattttccgc
    14461 atgattggta gtggtaatgt tgttttctaa gacgcaaata ggtttattta ttcccactgc
    14521 gatttaagat aatcattgaa atcttcttgc cgtttttcac gagctgcttc gcagtttctt
    14581 tacaaaatag acaatgtcta aaagtgcgaa ctaatgtacc ttcgattacg atggctttct
    14641 tgtccttata aaaagttttt tgaaaagaat taatttcaat atttttatct ttaatagtga
    14701 agtgctcttt gatagaacga gaaatggata tacaaaatct tcctttcaga ttgatgtttt
    14761 agacgactct attttatcga aatttttgta tgtctgtttt ttttacaaga aaatctagga
    14821 tcgacagaaa attctatcag tcctagaaat tatagagcca tttttttacg tcttatccag
    14881 atatattgtt ctttcattgt ttaaaaaata ttatttatca ctatttaaag aattaagttc
    14941 gttctttatt tgagttgtag agatctcagg tgttcttggt aaataaatta cttgtgcatc
    15001 tgttagttgt tcaagttcat caaattgtcc agcccaatca tcgcccatca cgaaataatc
    15061 tgcatggtat tcttttatat cttttacttt ttgttcccaa ttttcttcag gaataactaa
    15121 atcaacgtag cgaatagctt caagtaactg ttttcttttt tcataactaa aataacattt
    15181 tttttgtttc gcattccaat tgaattcatc tgtagaaagg gcaacaataa gataatctcc
    15241 atattgtttt gctcgtttca gtaaattgat atgcccgtag tgaagcaaat caaaggtacc
    15301 gtaagtaatt actcttttca tctttcacct ctcgaaattc attaatatac attgttagtt
    15361 tagcattttt ttgttagaat tgaaagaacc agtttatcct aaaaaggata agaaatgttt
    15421 atgagaaata ggtggatgga ggaaaaatgt tgaatgaaaa atttttaaaa tatgtgaaag
    15481 gttggtattt tatttatttt ggttttattc taatgttacg agcaataggc ccgtatttac
    15541 tattacctac tagatatgat actttacttt tttggcctgc tggaatagcg ggagttgtct
    15601 tgatcattat agatgcaatt attgcattta gagaaaaaaa tcttaaaaaa tatgattggc
    15661 tattaattgc ttttattggc gcgatgctag taagcagttt aatgaattta aaatatggat
    15721 atagcgaaaa tatcaagtta atcatgtggc aattaatctt tttctttatg atttatcagt
    15781 ttggcaaaga ctacagtagt aacttttttt tcaaagtatt tacttggata ctttcattgg
    15841 tatggttttt agctaattta gtatcattat actggtttgt cattcaatat ggcataaaaa
    15901 ttccaataaa atataagtat tacccagttc gtttagggtg gttaggcaac cgtttatttg
    15961 gtgtatatac agatccaaat tttggtgcgg ttatatcatt aattgtcatc attattgcta
    16021 tatattattt ggtcacttac aagcatttaa atcttctatt taaaatattt tttgcaatta
    16081 atatttttat tcaattctcg tttattgctt tatcgggctc acgcaccaca ttagtttgtt
    16141 tattaatagc aacttttgtt tttagtttct ttacaagtta tagtagtcgt tttcttaaaa
    16201 ctaatacttt aaaaagatgg ctaactgcat tagtagtagg attgctaagt ataattgcga
    16261 tgtattatgt aacagatata actgctcaag catgggcaca tgttccgcat attacagaaa
    16321 taaaaaaaga tatatctgat tcatcaggaa caactagcag caataaagtt gatttaaaac
    16381 gccctgacac tgatggcggg gatgtttcta atttaaggtt tggactttgg aaaagtgcaa
    16441 ttgaaatatt caagagtaag ccgatatccg gcggttctcc aggatactat attgaatacg
    16501 cacatgataa actaccatat acattaatgg gaaaagatac tttaactgct cataatttta
    16561 ttttcttagt tatggctgct acaggaggat taggtctatt gacattgtta accttcttat
    16621 ttatccaatg ctttaaaact ttgcgttatt gtttcacaca accaaatcta gtaaaagata
    16681 agcacttttt tgctatttta atgattctta caatagcagt ttcggcatta tttatcacag
    16741 aactcatttt ggttagtacg ataggttcat ttattttttg gggatatctg ggttctatcc
    16801 aaaaaactat atctaaagaa gaaggagtaa ctaattgaaa ctatttttag gtatataaag
    16861 tagaaaagaa aactagttat cccaacttca acaaatttta tagtttttac aatataaaaa
    16921 tggtaaacta actttattaa gtatgagaat gcataattgg agtgaatttg atggatacta
    16981 tattgattac tggtggagct ggttttatcg gttcaacgct agcgaattat ttgggaaaag
    17041 aaaacaaaat cgtagtagtc gacgatttat caatgggaaa aaaagaaaac ttggatatgg
    17101 agaaacaagt gactttcatt gagggggatg tatcagatcc tcaattgatg gaaagaatca
    17161 tgaaacagta tcagtttgcc tatatttttc atttagctgc tgtagctagt gtggcagatt
    17221 ctgtagaacg tcctctagaa actcatcgtg taaattttga cagtgcgctt ttattgcttg
    17281 aattggtacg aaaatatcaa tctggtttaa aacgattggt atttagttca tcagctgcag
    17341 tttatggcga tgaaccaacg ttgccaaaaa aagaagaatc ggtcatccgt cctttaacac
    17401 catatgccat cgataagttt gcatcagaac aatatgtttt ggattactgt catttatatg
    17461 atgttccgac tagtgctgtc cgatttttta acgtttatgg tccaaatcaa aatcctaact
    17521 ctccatatag cggagttatt tcgattctag tggaccgata caaaaaacaa ttggcaggag
    17581 aaaaaacaga atttacttta tttggtgatg gtagccaatc tagagacttc gtttatatag
    17641 aagatgttat acaagcactt ttacttgttg ctaaggaaga aaaagcttta ggacagcaat
    17701 tcaatgtcgg tacaggaaaa agcactacgc tacttgaatt gattcattca attgatcaaa
    17761 tcttaggaac agagctagcg cttaaatatg aagcggagcg ttcaggggat attcgtgatt
    17821 ctttagcgga tatttcaaag atccgttctt taggttatca acccaaattt gatatattga
    17881 acggaatgga acgttattta aagacagaaa tcgattgaaa caaaaaacca gcatcctgct
    17941 ggtttttttg tctcgactta gctttttaaa ttaacacctt ccatcagttc gtaaatctga
    18001 tttcttaaat cagtggccgg caatttgatg tttttatcag attggatatt actttccatg
    18061 acgattgcat cttttgtgtc ttttttcatc gccgctactg catggaatcg attgatattt
    18121 ccctgagcaa ggatataatc atttccactg taaacaccgc cgctgtaagg atgtttatca
    18181 ccattatttt gccacaatga gaaaagtagt tcttttggta taatatcgcc agacagaaca
    18241 ctggtaaaga atttgttcaa gtcataaaca gacatactta tatttcctgt gcctaattca
    18301 tttcggatgt ctgtttcagg ctcgaccaat gcttttgaat aatcgtcagt tgcagacatt
    18361 tggtaagata cagcatgctg aggggaattt tttacatttt cataaaaatc tgtatgttct
    18421 agatggagtg gccgaatgat ttcttttttt aaaagagttt cataaggctg attcattagt
    18481 ttgatcaata cccctgtaag caatgtataa tttaatggtt cataatgata cgtatcataa
    18541 ccagtaaatt tcaaatgatg aagggcaaac tgaatgatct cactatctga cataggtttt
    18601 tttggagaag cggttcgatg aagaccagag cgcatgtaaa gcatattttt gattgagatt
    18661 tgctgacttc caggaatctg aggataaaat tcagatagct ttgtgtcaaa agttatcttg
    18721 ccagattgaa tttgttttag gataagtaaa gcagtcatcg ctttttggat cgaaccgatt
    18781 tgatagtaaa gagaaggaga attcgattct tttatgcgta gatttgcgta cccatatcct
    18841 ttttctaata tcactcgatt atttttgcgt acataaatcg aaccgatata atctgatttt
    18901 tgcagtaatt gatcaatttt gctggcaagc atctgctcat tagatgttaa attggaagca
    18961 agttgtgcgg gttccgtttt ataaatttgt ggttgaggtt gttttgttaa ttgccatcct
    19021 gtaatgatgc ctgttgaaaa gaagctgaca gccagcaaca aaactgcgag aaaagaaaga
    19081 gagatctgct gttttttttg atgttttcct tttgccatat taaatccttt ctatccattt
    19141 ttaactattt ttcattgtaa tcctcaaaaa gagcatttgc cacttacaat tttgacataa
    19201 tatttggtaa aataattcac ggcagtagaa atttcatgta taataaacag agttacgaag
    19261 atgatagtaa ggaggagttg ttttgtcagg aaacaaacaa cacccaagca aagacaaaaa
    19321 agaagccagt aaggcagcta cagcagatat aatcgacaag cggagaaatc tgcgtaaaaa
    19381 agaagataag atcgtgagga agatcatttt agtgatcgct cttacattat tgattattgg
    19441 cggatttctc gggtttaccg tatatcgtta tgtagatagc gggttgaagc ctttagataa
    19501 atcggacgac caattagtcc aagtggaaat cccaaacggc tcttctaata agcagattgg
    19561 cgaaattttg gagaaagaca atattattaa aagtgggatt gttttcaatt attatacaaa
    19621 atttaaaaac ttaactggct ttcaagctgg ttattatcag ttagcgccta atatgactct
    19681 tgatgagatt ggcaagcagt tgcaagaagg cggcacatct gagccaacaa aagttgcaga
    19741 tgggaaaatt gccattccag aaggctacga catcgatcag attgctgagc gggtagcgaa
    19801 ggtaacaggt aaagacaaga aagaattttt agatttagta aatgatgaga ctttctttaa
    19861 cgagttgtat caaaaattcc cagaattgtt ggaaagtgcc tctaaagctg agaacacaaa
    19921 ataccgtttg gaaggctact tattcccagc tacttatgat tactacacga gtacttcgct
    19981 taaggatctt gtgattgaga tggtgaacaa gacaaatacg gtcatgcaaa actattattc
    20041 agctatcaaa cagaaaaatt tgactgtaca agaagtttta actttggctt ctctagttga
    20101 aaaagaaggc gtaaaagaaa atgatcgtaa aaatattgca caagtcttct ttaaccgaat
    20161 caaggcaaat atgccacttc agtcagatat ctctgtttta tatgcattag gagaacataa
    20221 agaattggtg acgtatgaag atacagctgt agattcacca tacaatttgt ataccaatac
    20281 cggctatggg cctggtcctt ttgacaatcc aagcgaaaag gcaatcaagg cagtattaga
    20341 accagcagaa aatgactatt attatttcgt agcagatacg agcactggaa atgtttattt
    20401 cgctaaaacg tatgaagaac atatggaact agtgcaaaaa tatgtaaata attcttagaa
    20461 aaacaggggc tagtcattga tcatttttag aaaaacattt acattttaag aaaagcgtgg
    20521 taatcttttt tggactatat tcctcaaaag attacctttt gcggtatttt atcgaaaaaa
    20581 ggagacgtta cacatggtag agaaagttta tccaatgact ctagaaggta aagaaaaatt
    20641 agaaaaagaa ttagaagaac taaaaacagt taaacgcgga gaaatcattg aacgaatcaa
    20701 aatcgctcgc ggtttcggag atctttccga aaactccgaa tacgaatcag ctaaagatga
    20761 acaagcattt gttgaaggaa gaatcacaac aattgaaaat atgatccgtt ttgcacaaat
    20821 catcgataat gacggcgtag atgcagatga ggtatcgatc ggtaaaacag ttacgttcat
    20881 cgagttgcct gatggagatg aagaagagta cacgattgtc ggaagtgctg aagcagaccc
    20941 gttttctgga aagatttcca atgattctcc tatcgcgcaa gcattgattg gcaaacgtaa
    21001 aaatgatgaa gtaaccattg ctacaccagg tggcgatatg ttagttaaaa tcgtcaaagt
    21061 agaacaagcg tagtctgact gaaagatcta gagagttcat cttactgatg acgcactcta
    21121 gatcttttct ctttgagtat tgcaggtttc actgaacgat cttacatcta aagtcctata
    21181 gccaacagtc aaaaaaatag gtatgatgaa atgagaatgt gtcaggaggg agaaaaagcg
    21241 atgaatagtt cttggcgttt ttttgtggta gtcgaagcac tgctgctgtt atttgctgtt
    21301 tggcaaatcg taaacaatac tggacttttg cttttagtaa tattcggtat tttcaatata
    21361 tatctagcta tgcgcaaata tcctcgtact aaatttcaaa attttcaatt gatcttaggg
    21421 agcttggtca ttttttttag cttagtcaac agtcctgctt tatggatgat ggttgtattg
    21481 gccgttttat ttatcgggct gaaaggtgta gagatttcag gaatagattt gacaaaaaat
    21541 gctttttggc gaaaaaaaca gatcatgatg gtccaaacag agcaattaaa aacacataat
    21601 aatgagcgta aaagacagca attatttggt aaccaacgta ttggaaacga cgtctatgaa
    21661 tgggatgata tcaacattgc catcatttct ggtgatacta tcatcgattt gggtaataca
    21721 cttttgccaa aagatgataa tattgtgatt gtaagaaaag ggataggcag aacacggatc
    21781 ttagtacccc tgggagtggc tattcgttta gagcatgcga cattagtagg aaatgtcttg
    21841 tttgaagaag agcagttttc cttaaaaaac gaacagatca aaatctgcag cagtgattat
    21901 gatgaaaatc ctcgcagatt gaagatcatt accaatacct tggttggaga tgtcgaggtg
    21961 atacgaatat gatgggaaga atatccaaag caatgctagc tgtttattcg gggattgcgg
    22021 ctttccttat tattctattt tcacttttca cttattttta tgccagtaac caaagtcatt
    22081 ggtggggaga attgctgcgt gcgcgtttat tatacgttcc gcttatcttc catttgatgg
    22141 caatttcatt aggtgtagga ttgatcgtat ttctattgtt atcacttatt caaaaggcaa
    22201 aatacgggaa aatcgaagaa aagcttcgtg cactttcttc tggcaattat gaatccaaac
    22261 ttttgcatca gccgatccca agcgcgtcgg atgatctgta catcaaagat attgatatgg
    22321 aaattgctaa aataaaagaa aaaatgatcg aagtatccag tgaattacag attatgacta
    22381 gccgtcctca atatgtagat ggacaaacca aagaagagat tttagaatta gaaaggcatc
    22441 gattagctcg tgagcttcat gattcagttt cacagcaatt atttgcagcg atgatgatga
    22501 tgtcggcatt gacggagcaa gcagaaaaaa gtgagacacc agaaatgttc cgtaagcagt
    22561 tgaaaatggt ggcagagatc atcaacgctt cccagtctga gatgcgtgcg ctgcttcttc
    22621 atctgcgtcc agtcaattta gaagaaaaaa gtttaaaaca aggcattgag cagttattga
    22681 aggaattgca gaataaaatc cagatttcgc taaaatggga tgtagaagat gtaaaattaa
    22741 ctagttccat tgaggatcac ttgttccgaa tcgttcaaga attgttatca aacacattaa
    22801 gacatgctaa ggctaatgaa ttagaagtct atttgcataa aatagataac aatcttttat
    22861 tacgtatcat tgacgacggg acaggattta atatgaatga aaccaaaacg ggaagttatg
    22921 gattgaacaa tatcaaagaa cgagtagctg gtattggtgg aacagtaaaa atcattagtt
    22981 ttaaagggca aggtacgagc gtggagatca aggtcccttt gatgaaggag gcatagataa
    23041 agatgataaa agttttatta gtagatgacc atgaaatggt acgcttaggc gtctcttctt
    23101 atttatctat ccaagaagac attgaagtga ttggagaagc tgaaaatgga cgtcaaggct
    23161 atgaaaaggc gatggcactt cgaccagatg tcattttaat ggatctagtc atggaagaaa
    23221 tggatggcat cgaatcaaca aaagcaatct taaaagattg tccaaaagcc aaaatcatta
    23281 tcgtcacaag ttttattgat gatgaaaaag tttatcctgc aattgaagca ggagcagcag
    23341 gatatttgtt gaagacgtct accgcacatg aaatagctga tgctataagg gctacgcaac
    23401 gcggcgagcg agtattggaa cctgaagtga caacaaaaat gatggaaaaa atgagtcggc
    23461 gaaatgatcc agtattgcat gaagaattaa cgaatcgaga aaatgaaatt ttgatgctga
    23521 tttctgaagg gaaaagtaat caggaaatcg cggatgaatt gtttatcaca ctgaaaacag
    23581 ttaaaacgca tgtgtcgaat atattagcta agttagaggt agaagaccga actcaagcag
    23641 ctatctatgc gtttaaacat ggtttggtga aataaaaacg atgataaaga ggataatcaa
    23701 aagctgagaa aataactatt atttttcttg gcttttttac aataatgatg cttgcttttg
    23761 tttttggaaa ggctttgtta tactgagaaa agaagatact aaaggaggaa aatggtgaaa
    23821 caaaattttg caattgtagg ccttggacga tttggcggaa gcatctgccg tacactgatc
    23881 gaagctggac aagaagtact ggctattgac agtaatgaag atcgagtcaa tgaatatatg
    23941 aatatagcca ctcatgctgt tgtggcaaat gcacaggatg agatgacact tcgttcatta
    24001 ggtataagaa atttcgatca tgtaattgtc gctattggag aagatatcca agccagtatc
    24061 ttagttactc tgatggtaaa ggaaatgggc gtaccgaata ttttagcaaa agcccaaaat
    24121 gagtaccatg cccgtgtact agagaagatc ggagcagatc gtgtcgtaca ccctgagcga
    24181 gacatgggga tacggattgc acataactta gtatcaaaga atattctaga ctacttagag
    24241 ctgtcagata aatattcttt agcagaaatc cgagtctcca atcctaaatt tttcaacaaa
    24301 acattagctg aattaaattt caggcaacgt tttgacttaa cagtagtcgc catacgaaga
    24361 gtagatcaaa cggttatcgc ttctcctgca gcagatgaat atgttcgcga aaatgataat
    24421 ctactggtga ttggagagac aggcgatgtg gatattctag acgataagat gaatcaatag
    24481 aaagaaaaga gaaagtaggt tgttgggatg gaattaacgg taacgccaaa agcacaaaag
    24541 tggtttgact cagaaattga tctgccagaa acttacggaa tacgtttttt tggaaaagtc
    24601 tacggcaaaa cagaggttca tgatggtttt tcgattggaa tgtcagttga acagccagaa
    24661 catcctgtaa agaaagtgga aatggatggg acactttttt ttatcgagga agcagatgaa
    24721 tggttcttta aaggatatga tttacttgtt gattatgatc ccaacctgga ggagccaacc
    24781 tatcacttta aaaaacaata aaaaaagaga gggtgacaca agtttt
//
LOCUS       DACCDF010000039        23650 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCDF010000039 DACCDF010000000
VERSION     DACCDF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.040"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            248..523
                     /locus_tag="IVK51_001556"
     CDS             248..523
                     /locus_tag="IVK51_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431155.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            536..2614
                     /locus_tag="IVK51_001557"
     CDS             536..2614
                     /locus_tag="IVK51_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6431156.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2774..4660
                     /locus_tag="IVK51_001558"
     CDS             2774..4660
                     /locus_tag="IVK51_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6431157.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4672..5619
                     /locus_tag="IVK51_001559"
     CDS             4672..5619
                     /locus_tag="IVK51_001559"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6431158.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5639..6166
                     /locus_tag="IVK51_001560"
     CDS             5639..6166
                     /locus_tag="IVK51_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6431159.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6229..6882)
                     /locus_tag="IVK51_001561"
     CDS             complement(6229..6882)
                     /locus_tag="IVK51_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6431160.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7014..7856
                     /locus_tag="IVK51_001562"
     CDS             7014..7856
                     /locus_tag="IVK51_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6431161.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8014..8910
                     /locus_tag="IVK51_001563"
     CDS             8014..8910
                     /locus_tag="IVK51_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6431162.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8913..9626
                     /locus_tag="IVK51_001564"
     CDS             8913..9626
                     /locus_tag="IVK51_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6431163.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9646..10164
                     /gene="msrA"
                     /locus_tag="IVK51_001565"
     CDS             9646..10164
                     /gene="msrA"
                     /locus_tag="IVK51_001565"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6431164.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10161..10388
                     /locus_tag="IVK51_001566"
     CDS             10161..10388
                     /locus_tag="IVK51_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6431165.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10540..11091
                     /gene="lepB"
                     /locus_tag="IVK51_001567"
     CDS             10540..11091
                     /gene="lepB"
                     /locus_tag="IVK51_001567"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6431166.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11174..11716)
                     /locus_tag="IVK51_001568"
     CDS             complement(11174..11716)
                     /locus_tag="IVK51_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6431167.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11953..12351)
                     /locus_tag="IVK51_001569"
     CDS             complement(11953..12351)
                     /locus_tag="IVK51_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6431168.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12611..13471
                     /gene="ylqF"
                     /locus_tag="IVK51_001570"
     CDS             12611..13471
                     /gene="ylqF"
                     /locus_tag="IVK51_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6431169.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13464..14231
                     /locus_tag="IVK51_001571"
     CDS             13464..14231
                     /locus_tag="IVK51_001571"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6431170.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14287..15144
                     /gene="dprA"
                     /locus_tag="IVK51_001572"
     CDS             14287..15144
                     /gene="dprA"
                     /locus_tag="IVK51_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6431171.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15266..17344
                     /gene="topA"
                     /locus_tag="IVK51_001573"
     CDS             15266..17344
                     /gene="topA"
                     /locus_tag="IVK51_001573"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6431172.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17313..18689
                     /gene="trmFO"
                     /locus_tag="IVK51_001574"
     CDS             17313..18689
                     /gene="trmFO"
                     /locus_tag="IVK51_001574"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6431173.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18887..19792
                     /gene="xerC"
                     /locus_tag="IVK51_001575"
     CDS             18887..19792
                     /gene="xerC"
                     /locus_tag="IVK51_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6431174.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19829..20377
                     /gene="hslV"
                     /locus_tag="IVK51_001576"
     CDS             19829..20377
                     /gene="hslV"
                     /locus_tag="IVK51_001576"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6431175.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20391..21791
                     /gene="hslU"
                     /locus_tag="IVK51_001577"
     CDS             20391..21791
                     /gene="hslU"
                     /locus_tag="IVK51_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6431176.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21809..22600
                     /gene="codY"
                     /locus_tag="IVK51_001578"
     CDS             21809..22600
                     /gene="codY"
                     /locus_tag="IVK51_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6431177.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22682..23557
                     /locus_tag="IVK51_001579"
     CDS             22682..23557
                     /locus_tag="IVK51_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6431178.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8192 a         4078 c         4981 g         6399 t
ORIGIN      
        1 cagccccatt tttttgttgt gataaaagaa caagaaaggc aggaatggga aagtaaacat
       61 ccttttgtca gaaactttta tctttcttaa gttttacttg atatctgata ctatttggaa
      121 tagaatgagg cgttgaaaaa tttatggaaa tttctaacta gcaataaacg atgaaacaat
      181 gaaatatttt tcatccaaaa agaactttgc tataatgatg gaagtttgtg tataggagat
      241 ctggaaaatg agagagagtt ttgaacaaca aaaaaaactg ttgcatgatc gctatggcgc
      301 cctttcgatg gatgaccgcc gtcaaatact ttgtaagttg cgtaaaagaa atatattgat
      361 gtatcgtcag ttggaacgtc tgaaacatga tctgcttcgt ttggagtcaa aacgtgtaca
      421 gtgtgaatta gaggggaacc aaacacaggt tgaagtagtc gaaaccaaaa ttttaaagaa
      481 aaaagaacaa tttttaaaaa tgcttacaca aaataaaaaa taaaggggat ggacgatgga
      541 atttgctgag ttagttattg tatttgcagt aacgatcacc ttatcgaaca tagctagccg
      601 cattttgcca atgattccag ctccactgat ccaaattttc cttggagtgg tattaggtct
      661 gacagagtgg ggacaatcaa tcgattttga acctgaattg ttcttagtta tgattattgc
      721 accattgtta tttagagaag gagaaaaagc agatatttct tcgattctca aaaatttcgg
      781 gacaattttg ttcttggcat ttggaggagt cattttgacc cttgtcgggg taggtgcgac
      841 gttatcattc ctgttgccaa gtgtcccact tgctgcctgc tttgcttttg gagcagcgtt
      901 aggtccgaca gatgcggtag cagtcagttc tctttctgga cgagtgaata tccctaaaaa
      961 ggcgatgcat attttggaag gagaaggtct tttgaatgat gcatctggtg ttactgcctt
     1021 ccagtttgct cttggtgcgc tgatcaccgg atctttttca gcagtaaacg cggggatgag
     1081 tctagtagtt tctagtattg ggggagcttt gatcggcttt ttgcttgtct ggttcaaaca
     1141 aaaaataatt catttgatcg aaaaagcatc agcacaagat gtcacagggt atctgctgat
     1201 cgaacttttg cttccttttc tagcctatgt attggcagag gcatttgatg tatctggaat
     1261 cattgctgca gtggcagcag gtattctcca agcttctggt tttaggaaaa tctctgtgtt
     1321 tgatgcagaa ttgtcgagct tgtcccacag tacatggaca actattgctt ttacactgaa
     1381 tgcattagta tttatatttt taggaatcga attgacgcaa gtgttctcgc cagtatgggg
     1441 agacggttta tatccaaacg gtcttttatt agcgattatc gtattaatct ctgtcatgct
     1501 ttttgtgatc agattcattt ctatcagtct tttttatgta ttcaaagatg gcagtaaaaa
     1561 gttcaaaaaa cagttgaacg agatcttgat cttaacattt ggcggtgtaa agggaacagt
     1621 cagtttggct acgattttta ttttgccatt ttcaatcaat aatatgatgt tttaccagcg
     1681 gtctttgctt ttgtttctga cggcgggtgt catcttagtg actttggtca tagggatcat
     1741 tgtcttgcct atgctgacgg aaacagagga agcgaaaagc acggatttga atgctttgat
     1801 gattttagaa gaggtcgttg aagttttgcg aaaagaaata aaggaaattg atcgtaatac
     1861 aaaagaattt ttagccactg aagcagtgat agaaaattat caagaacgga tacgtgatct
     1921 gtacttggaa gatttaaccg atgatgaaaa gcaagaagta caagaaatac aagcactgat
     1981 tttgtcgatc gaacgggatg gattggatga aagttatcgt tcgggcaagc tttcttctaa
     2041 tggttatcgt ttttactccc gttttctttc tcgattcgaa cattctatca caagccagat
     2101 tttgtctttt atcggctttt ggttcattgt tgtccgtagg ttaatgcgaa tcgttctcca
     2161 tccgaaaatg ttttggcaaa gaagatatgc tgatcgtcag acatttatta gtgatgaaga
     2221 tatccaagaa atcagagaga catatcaaaa aaatactcag ttgattattg aaagtttaga
     2281 taacttgact gatatttatg atgaaacgat ggttcaattt tttatccagc aacgcaaact
     2341 tgaaggaata aaaatgaact cggggaattt gatttcttcg tggatgatcc agcaagatag
     2401 tctgtttacg aaaaaaatgc taagaggtta ttatcttgaa agaaaaatca ttgatgaata
     2461 cgaagttgct gaaaaaatca cgacgttttc tgcgaacagc tatcgcagga atatcaattt
     2521 gttagaatct tatacgatga acaaaccatc tgataacttt tctttccgat ttgcgtttcg
     2581 agcaaagtca aaaaaaacga gtccaataaa ataaaaaggt aaaaattttc aagttttata
     2641 aaaaacaaaa catatcagaa caggatcgaa tcggtcctgt tctttttttg agaacacaaa
     2701 aagaacaaat accaaaaaaa ttttcctgaa gttcactatt tttatgatac aatatccaag
     2761 ataaggaggg aacatgttga aagaattaaa agtaactgac ctcaaaaaaa cttatggtga
     2821 aaaagatttg tttgaccaga tttctttttt ggtccacgaa aaagaccgta tcgggttgat
     2881 tgggacgaac gggacgggta aaacaagtct cttaaatatt attgccggta tcgatagcag
     2941 cgacggtgat cggcaaacag tgttttatcc aactgattac cgtattggtt atctttctca
     3001 agcagctgaa ttttccgatg aattaactgt tctccaagca gtctttcaag gaaacagccc
     3061 cttgatccaa actgttcgag catatgagga agcattgatc gaactagggg aaaacggaga
     3121 agaccctgat gtgcaaaaac gttatgctaa agcagaagaa caaatgaata aagaagatgc
     3181 ttggactaca gataccaacg caaagattat tttgcaaaaa ttaggaatca gtgaattaga
     3241 taaaaaaatc agtactttat caggtggtca gaaaaaacga gttagtctgg cgcaagtgtt
     3301 gattgatgaa cccgatttac tattactaga cgaaccaaca aaccatttag attatgaagc
     3361 gatcgagtgg ttggaaaact atttgaaaca ataccgtggc gcactattga tggttaccca
     3421 cgatcgttat tttctagata gagtagcaaa ccgaatcttc gaactttcat ttggaaaatt
     3481 atacgagtat aaaggaaatt atgaagctta cgtcttagaa aaagcagaac gtgatcgtgt
     3541 agaagtagaa caagaagaaa agcgcaagcg gctttataaa caggaattag cttggatgag
     3601 ggctggcgta caggcacgtg gcaccaagca gcaagcacgg atcaaccgtt tcgaagatct
     3661 aaaggagaac ttgtaccaag taaaccaaga agatgattta gagctgaacc tagcgacaca
     3721 acgtttaggg aaaaaagtac tggaaatcaa agatggcagt taccgtataa atgaccaaac
     3781 actgctagaa catttagatt tattgattca atctcgggaa agactaggta ttactggtaa
     3841 aaatggtgcg ggaaaatcga cattgcttaa tattctagct ggcagaattc ccctggatag
     3901 cggtacaatg tctatcggtg aaacggttca tcttgcgtat tatacacaag aaaatgaaga
     3961 aatggcacca gataaacgga tgattgctta cttacaagaa gctgctgaag aagcaaaaac
     4021 agcagatggc agtcaaatcg gagtagcaga attacttgaa cgatttttat ttccaagatt
     4081 tatgcatggg acactgatag gcaaactttc aggcggagaa aaacgccggt tatttttgtt
     4141 gaaactgctg attcagcaac ctaatgtttt attattggat gaaccgacca atgatttgga
     4201 catagcaaca ttgacgattt tggaagatta ttttagaagt tttccaggtg ctgtgatcac
     4261 tgtttcccat gaccgttatt tcttggacaa ggtagcagat aagttattag tattccaagg
     4321 aaacggaaaa caagaacttt attacggaaa catgtccagt tatttactga agcaaaaaga
     4381 gacccagcag cctgctgaaa aagcgaaacc aaaaatacag agcaaggaac ctgctgaaaa
     4441 gaagaaatta tcttacatgg aacaaaaaga atgggaaacg attgaagatg agattgcaga
     4501 acttgaagaa aaaatctcat tgctccaaga agagatgaat catcaaggag ataatttcac
     4561 tcgattacaa gaattgcaaa acgacgtatc tgaaacagaa gcacaacttg aagaaaaaat
     4621 ggcacggtgg gaatacttga gtgaatgggt agaagattag gaggaaaaag gatggaacaa
     4681 gcgtatcttg acttaggaag aaaattgttg gaagaagggc atcgtaaaga agacagaaca
     4741 ggcacaggca cattaagctt attcggatac caaatgcggt ttgatctgca aaaagggttt
     4801 cctttgttaa caacgaaacg agtacctttt ggattgatca aaagcgaatt gctttggttt
     4861 ttaaaaggag atacgaatat ccgctatcta ttacaaaata aaaaccatat ttgggatgaa
     4921 tgggcatttg aacgctacat caaaagtcca gactataatg gaccagatat gactgatttt
     4981 ggccatagat gtttgacaga tcctgaattc aatgaagaat ataaaaagca gagcaagctt
     5041 ttttgcgaga agatcctaac cgatgattcc tttgctgaaa catacggcga tcttggacac
     5101 atctacggct atcaatggcg tcactgggaa acaaaagacg gtggctttat cgatcaaatc
     5161 aaacaagtga tcgaagcaat caaaaagact cctgattcta gaagattgat cgtttctgca
     5221 tggaatccgg aagatgttcc tagcatggct ttgcctcctt gtcatacgat gtttcaattt
     5281 tatgttcaag agggacggct aagctgccaa ttgtatcaac gcagtgggga tgtgtttcta
     5341 ggcgttccgt tcaacattgc aagttatgcg ttgctgactc acctaattgc acatgaaaca
     5401 ggtttagcgg ttggcgaatt tgttcacaca ttaggagatg ctcatcttta tatcaatcat
     5461 atcgaacaaa tgaaagaaca attgagccga gaaatacgcg catttccgac tttagttttg
     5521 aatcaggaaa agcagtcagt atttgatttt gagatggaag atatccaatt agaaggatat
     5581 gacccacatc cggcgatcaa agctcctatc gctgtttaga atcagaaagg atgagaaaat
     5641 gttagctgca atttgggcac aggatgaaaa cggattgatt ggaaaagaag accgtcttcc
     5701 ttggcgtttg ccaaatgatc tgaaattctt caaacaaatg acagaagcaa atacactagt
     5761 gatgggacga aaaacattcg aagggatggg tagccgtccg ttgccgaatc gtcagacgat
     5821 tgttttgacg agagattctt cctataaagc agaaggcgtg catgtcatgc atagtgtgga
     5881 agacgtgctt gctctggaaa aagaaacaga cggtatcttt ttcatcgctg gagggtcagc
     5941 tgtttatcaa gaatttctgc cattttgtac gatattatac cgtacagtca tccatcatgt
     6001 attcgatgga gatgcttatt ttccacctgt tgactggtct gattggtcac tgatcaatac
     6061 tagtcaagga gaaatggatg aaaaaaatcg ttatccgcat caatttgaaa cgtatcagcg
     6121 taatgagaat cctgtaaaaa gcgggacagg cgaattgacg atataaaaaa gctatggata
     6181 gactagctgt tatttagagc tgtcccatcc atagctttat tttttttatt aagtaaagaa
     6241 taaaacagag aagtacataa aacctgcacc taacataaca aacaaatgcc aaacaacatg
     6301 catatagcgg atagaattca agctataaaa gaatgcgcct atcgtgtaag aaagcccgcc
     6361 tgctaccatc aaaataacac cagtcattcc aagagagtga taaagaggaa cagcagccac
     6421 gacacagagc caccccataa aaatatagat gatcgtagat acttttgata ctctctcccg
     6481 tttatgcaac gtcaacgctt tatatataat gccaagaatc gcaaacaacc agattaatac
     6541 aaataaagtc caacctagcc atcctcggat actcagcaaa caaaaaggag tataacttcc
     6601 tgcaatcaaa agaaaaatcg aactatgatc gaatacttgg aacacctttt ttgcttttgt
     6661 aaaaatcaat gaatgaaaca aagtagacgt taaaaataaa agaatcatca tcgatccata
     6721 gatagcataa gagacaatat ggagggagga atgcaagtgg gcacctttta ctaaaagaac
     6781 cactagtccc gcgatactca atccagtccc aattccatgg gtgactgcat taaacacttc
     6841 atttaatatt tcatacgttc gattaaattt cgttttttcc atgaaaacgg ctcctttact
     6901 aggtgtaatt aatgaaaaat cattatttgt ctgttatact aaaaatagaa attgtcttac
     6961 atgtagttta acatgaataa ggcatcttgt taaagaaaga gagagagaaa ataatgacaa
     7021 atgtaaaaat cgtaacagat tcttcatgta cgatggaaaa aaatgtacgc gatgctttag
     7081 gcattcgtgt catgccgcta tccatcatga tcgacggaac agtatatccc gatgatgacc
     7141 aactagacgg cgaaagattt atggaaatga tggcgaaagc aaaaaatctg cccaaaacaa
     7201 gccaaccacc aattggagaa ttcatctcga tgtatgatga gctaggagaa gacggcagtg
     7261 aagtgatttc gatccatatg acaaaaggct tgagtggaac agtagaagca gcaagacaag
     7321 ccagtaatct aacaaaaacg aaagtaacgg ttatcgacag tgataccact gaccaaggat
     7381 tatcttttca ggtaattcga gcagctgaat tagcaaaaga aggtaaaaca gtagagaaaa
     7441 tccttgaaga agtcaaaaaa gtacaagaca atacaaaatt atacatcgga atatccacac
     7501 tagataactt ggtaaaaggt ggaagaatca gccgaacaac tggtttgctg tctagtgttt
     7561 tgaacatacg cgtggtgatg aatttcgccc attctgaatt aatccctgta actaaggggc
     7621 gaggaaagaa aacgttcgat aaatggttta ataatttgaa aaatgaactg aaagaattgc
     7681 caaatgtgcg acaaatcggt atttcacatg cggatgcaaa agaattggcg caagaattca
     7741 aagagggttt gcaagaatta ttcccggaca tgcatatccc tgtactgcat acgaatccgg
     7801 ttatcgctac gcatactggt aaagacgcgt ttgcaatcat gtattatgta gattagtcga
     7861 aaagaggata agccctcagc ctgaaaggct tgcagcttat tctctttttc tttataaaat
     7921 taggaaaata attgttttct tttttcataa agactacctt tttgataaaa tttttgataa
     7981 cattagaacc aatcgaatac aagaggtgaa aaaatgaaat taacgaaacg acatttgatc
     8041 ctcgttttgt tcccattatt aattgcgatc atcatctatg caggtttagc tgcttctata
     8101 ccgaaagctg gcccactaat gaaaccagta actactgtga gagaagatcc aaataaaaaa
     8161 gaaaaacttc actacatagc tatcggagat tcgctgactg aaggagtagg agatcaaacc
     8221 aagcaaggag gattcgttcc ccttgtcgca aatgatctaa aagaccgtta taatttgacc
     8281 gcggttgaaa cagaaaacta tggtgtaaat ggtgaacgaa gtgaccagat cttaaagcga
     8341 gtaaaaacaa aagaagaaat tcgaaacaat atcaaaacag ctgattttat cacactgact
     8401 gttggcggta atgatttgat gaaagttatc caaaataatt tattcgggtt aacggtcaat
     8461 tcctttaaaa agccactgaa aaactatcag gaaaatgtca cagaactatt ggaagaattt
     8521 cgatccctca ataaaaccgc acctatttac attttaggca tctacaatcc attttatttg
     8581 aactttcctg agatcactga catgcagaca attgttaaca attggaacaa aggaacagaa
     8641 acggtgacaa aaaaaatgcc gaactgctac tttatcccaa tcaatgattt gctttatcaa
     8701 ggattgaatc aggaagtagg tgtcacagaa gaaggaacag atcaaacaac tagcacgaca
     8761 gaaaatctaa atggcatcaa aaataatgtg ctctatgaag aagatcattt ccatccaaat
     8821 aatttaggtt atcaattgat ggcaaatgca gtgagagatc agctgatcga gacacaaaac
     8881 caatggttag agaaggagga tgatcaataa acatgaaaac aagatctgac gtatcttcaa
     8941 acaaaacaaa gaaaacaaat tactggaaat tcgccttcct tatccttctt ggggtagtac
     9001 ttggttcgac cgtattttta ggcagccgta tttttgctaa tcgggaacca gaactcccag
     9061 aaataccagc tcttacggaa agacaaggag atcctgtctt aacgatcaat tcaaataaag
     9121 aaaaagtcaa tcaaatcatc agctttttcc ttagtgagta tcaaaaagat agtgatattg
     9181 aatataaatt ttatttggaa aatgaagcac tactcaatgg tactttcgaa gtactaggtt
     9241 ttcctatcga tttctattta tattttgatc cgtatgtgat ggaaaacgga aatgtccaac
     9301 taaaagctag aagtctttct atcggaacat tgagtttgcc aatcaaagat gtgatgaata
     9361 tgatcaagag aaactataaa ttaccagatt ggatcgaagt caatacagat gatctgacta
     9421 tcatgcttag gttggatcaa ttccgaatgc aaaacgggat gtatattaaa gcagataaaa
     9481 tcgacttagt aaatgatgac attcggttta gtctatattt gccaaaagat acctctgatg
     9541 cttcaaacaa ctcaaatacc aacaaaacta gcaccagcag tactgaaagc aagacaaaag
     9601 aaaataaaaa atcatcggat gagtagtatt agagggggaa tcataatgga acgagcaata
     9661 tttgcaggag gctgcttctg gtgtatgatc cagcctttcg acacgcttcc tggtatccat
     9721 acagtgatgt caggatatac tggagggcat gtaccagatc cgacttatga acaagtaaaa
     9781 agccaaacga ctggacatac agaagcagtg gagattatct atgatccaac attgatttca
     9841 tatgaacaat tagtagaact ttactggcag caaactgatc caactgacgc tttcggacaa
     9901 ttcgaggatc gcggtgacaa ttatcgcccg gtgatctttt atttgacaga agaacagcgg
     9961 atcattgctg aaaaaagtaa gaaacagtta gctgaaagtg gacgtttcga acagccaatc
    10021 gttacaaaaa tcgaaccagc ccaaccattt tatctagcag aagaagacca tcaagagtat
    10081 tataaaaagc atcctgaaaa ttttaaaaga aaccatgcac gccgggctgc ctttatcgaa
    10141 gaaaattgga ggaacgaagg gtgaatcgaa gcttttttca ttatttaatg acactgcgag
    10201 gcggtggcac acatgatgct ttatctgttt tcgctacaga agccggtaaa gacatccaat
    10261 ttcctaagca cagctctagc tacgaagaaa tttctaatta tctggaactg aatgtcgact
    10321 atttgccgtc catggatatc tttgacgaag catgggaaaa atatttagaa aataatcatg
    10381 acatataaga gatagttttg tactttatga tcaaaaaagt tttttgtcca atagcctaat
    10441 tgaaagaatt atttttattt cagctatcta gtcgcatttt tttgatattt ttcttataat
    10501 aaagaaagaa tgaaaaaaag agggatggat tgatagagat tgacaaaaaa acaacgctat
    10561 attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct tgcgttcatg
    10621 ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga aaacgtgttg
    10681 aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt tgatattgtt
    10741 gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct tccaggagaa
    10801 aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa ggaaccttat
    10861 ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga ctttacacta
    10921 caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact tggagataat
    10981 cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga tattctaggt
    11041 aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg agaaacaata
    11101 aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca gtttactacc
    11161 ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa gttttgataa
    11221 atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg aatctaccta
    11281 gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa ttgataaaaa
    11341 ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg tttatctttt
    11401 tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata agaaaagcag
    11461 cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac atttcaagca
    11521 aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa aatccgaatg
    11581 tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca agggctaata
    11641 aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa acgtcttttg
    11701 caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg ataccataaa
    11761 atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa caattaaaaa
    11821 aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat aaaaaaaata
    11881 gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa aaacgtcaga
    11941 ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt ctagatcttg
    12001 gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt ggaaagtacc
    12061 accattttct tttgcaattt gagcaaattt ttttacttca tccgcatttt caacactaaa
    12121 cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt ttataaattt
    12181 tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata ccattgaacc
    12241 agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat aaaaagcaac
    12301 ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca tttgtttcgt
    12361 ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca aaaatttaga
    12421 ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga tgtgatagaa
    12481 tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat aaaaaagtga
    12541 atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta tgaaaggaat
    12601 ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc gacgagaagt
    12661 ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac gattaccttt
    12721 atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc ttgttttatt
    12781 gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat attttcgtaa
    12841 acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta aaggtctagt
    12901 acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc gggggatcaa
    12961 gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat ctacgttgat
    13021 gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg taacaaaagg
    13081 tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag ggattttatg
    13141 gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg cgatcaaaga
    13201 ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg cccgatatta
    13261 ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa aattggcacc
    13321 ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg atcgtggcag
    13381 cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac tggatcgttg
    13441 cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa gaacgattga
    13501 aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat ccacgcaaag
    13561 gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca ttaattaagg
    13621 aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt caccaatgga
    13681 ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg gcagctgcag
    13741 ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa ttatcagaga
    13801 aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc gggatcggga
    13861 tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa ttagcgatga
    13921 tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat gcgatgacct
    13981 tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct gtgtcgattg
    14041 ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa tatgacgtgc
    14101 tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa cacttagaag
    14161 gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct gtaaaaaaat
    14221 atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac gaaaggcggt
    14281 tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa aggaatcagt
    14341 agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa agaaatcgga
    14401 tttgaagaca tcattaagat aggtaaaata accaaacacc gggagctgtt tcgcaaaagt
    14461 tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt tatcacttgc
    14521 tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat tgtactgttt
    14581 tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg tggaagacac
    14641 acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat agaagctgga
    14701 tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca agcagcaatc
    14761 tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt ctatcctgct
    14821 gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag tgaatatcct
    14881 cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat cgcagctttg
    14941 tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat aacagcacag
    15001 caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt aacagaacat
    15061 tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc gggtgaagat
    15121 atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt gacaaacgtt
    15181 ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag taggaacacg
    15241 acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg tagaatcacc
    15301 tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa tggccagtgt
    15361 tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa acaattatga
    15421 accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga aagccgcagc
    15481 aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag aagcgattgc
    15541 ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg tggtcttcaa
    15601 tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga tcgatgtaga
    15661 tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt attcgatcag
    15721 tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc aatctgtcgc
    15781 tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag aagaatattg
    15841 gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt tctggggaat
    15901 cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca ctgctagaat
    15961 tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa gaaaccctgc
    16021 gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa actttcgaac
    16081 aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg gtaaacaagg
    16141 aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg atacagcaaa
    16201 agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta gacatgatca
    16261 ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc gtccaacgag
    16321 tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat tgaaacttta
    16381 tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt tagatacaat
    16441 gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa aaatcaaatt
    16501 caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag agaaagaaaa
    16561 tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg aaccaaaaca
    16621 acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg cactagaaga
    16681 aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc aaagacgcta
    16741 ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa ttgtcaattc
    16801 attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag aaatggaagg
    16861 agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg atcgtttcta
    16921 taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa ttcaaatcaa
    16981 agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga taaaattagg
    17041 gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata cgaaaccaat
    17101 cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca ttgaacgaaa
    17161 atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg aattcacttc
    17221 ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg tcgagaagaa
    17281 agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag acatccaaaa
    17341 ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca gggcttgccg
    17401 gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta tatgaaatgc
    17461 gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta gtatgttcca
    17521 attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa gaaatgcgcc
    17581 gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct ggtggtgcct
    17641 tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa gagcatccat
    17701 tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc gtagtagcaa
    17761 caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat ggttcagatg
    17821 gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc gatatggata
    17881 aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac tgtccgatga
    17941 cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg gcagaactta
    18001 aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta atggcacagc
    18061 gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa gatcctaaaa
    18121 ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca gcttctttat
    18181 acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt gttttccgga
    18241 tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac cgaaacagct
    18301 ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc gaagatctgt
    18361 tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg agcggactac
    18421 ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag tttccacaag
    18481 aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa catttccaac
    18541 cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga gacaaaaaag
    18601 caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata aaaacagaat
    18661 tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg agacggatag
    18721 tctcgctctt tttcttgcgt ttttcttaag aatttctcaa acaaaatgtg aggaattgcg
    18781 ccaattgtga acaaacttag aaatattggt taaattctga caatttattt gtctttttat
    18841 aaacgctgtg ctaaattatt ctctgggaca aaaggaaggt gagaaaatga cagaagccac
    18901 gtgggaacaa aaatttttgc gttatttgat tgtcgaaaga ggatattcag aaaaaacgaa
    18961 agaagcatat gaagaagaca ttctccattt caaacatttt ttggaggaat ctggagacgc
    19021 tgatctgctg aaggcggaat attttacggt tcgtgtctat ttgagtgcat tagctgatca
    19081 tcgtcctagc tatagcagaa attctatcag tcgcaagata gccagcctac gttcgtttta
    19141 tcagtatctt ctcaaagaag aagtgataaa agaaaatcca ttttcttacg ttcatctcaa
    19201 aaagaaaaat ttgagactgc cacgtttttt ttatgaaaat gaaatgcaag cactttttga
    19261 cagcgtagca ggagatagtc ctttggattt gaggaaccgc gctttactgg aagtccttta
    19321 cgggtcaggc atccgtttaa gtgagtgtag cagtttgtcg gtatcagaca ttgattttga
    19381 ttcagaggtc atgttgatcc atggtaaagg gaacaaagag agatatgctc ctctaggttc
    19441 ttttgctcaa gatgctctgc aagaatattt cagggaaggc cgccaggtat tgatgacaaa
    19501 atatcacgaa gagcatgata tcgtttttgt gaatcatcat ggaaaaccaa tcacgcctac
    19561 tggaattgaa tatgtcttga atcaaatgat caaaaaaagc agtttggata gtgatatcca
    19621 tcctcacatg ctgcgccata catttgctac tcatttatta aataatgggg cagatttgcg
    19681 gacagtccaa gaattgctag gacatgccaa tttatcgact acccagatct atgcccatgt
    19741 aacaaaagaa agtttacaaa aaaactatcg ttctttccat ccaagagcgt aaaaataagg
    19801 aaaagaacaa atttaggagg aaacaaacat ggtcgaatca ccatttcatt caaccactat
    19861 ctgtgccgtc gaaaaagacg ggaaatttgc tatggctgga gatggccaag taacaatggg
    19921 tgaatctgtt gtcatgaaag gcactgcaaa gaaagtacga cgtatctaca atggagaagt
    19981 tgttgtaggt ttcgccggaa gtgttgcaga tgcgtttact ttagaagaaa aatttgaagg
    20041 caaactaaac gaatataacg ggaatctaca acgcgcagct gtcgaattag ctcaagaatg
    20101 gcggacacag caatctatgc aaaaacttga agcgatgctg atcgtaatga atgataaaga
    20161 aatgcttctt gtttcaggta caggagaagt tatcacacca gatgatggta tcttagctat
    20221 cggatcagga gggaattttg cgttggctgc agcccgagca atgaaaaact atgctcaaaa
    20281 cgacatgagt gcgaaagaaa ttgcagaaaa tgcgttaaat attgccgcag atatctgtat
    20341 ctttactaat cataatatta tcgtagaaga actgtaagag gtgagtagat atgtatgaat
    20401 taaaacaaac accaagacaa atcgtaagtg aattagatga atatatcgta ggacagcacg
    20461 cagcaaaaaa atctgttgcc gttgctttgc gtaaccgcta tcgccgtttg cagttagatg
    20521 aaaaaatgca acaagatatt acaccaaaaa atatgctgat gatcggacct acaggggtcg
    20581 gtaaaacaga aatcgcacga cgtttggcaa aaatcgtcaa tgcaccattt gtcaaagtcg
    20641 aagcaacaaa atttactgaa gtcggttatg tcggaagaga tgtagaatca atggttcggg
    20701 atttagtaga aaatgcgatc caaatcgtag aaaaagagca gtacagccgt gtatacacaa
    20761 gtgctaaaaa aaatgcagaa aaacgtttgg taaaaatctt ggtacctggt atcaaaaaag
    20821 aacagaaaca atccactaat caatttgaac aaatgatgaa tctttttaat gctagtcagc
    20881 aaaataccgc tgaaccgcaa gaagaagtga cagaagatat tcgaatcaat cgccgtacag
    20941 tcttggaaca actacaaaag ggtttactgg atagtcgtga agtaaccatt gaagttgaag
    21001 accaaaagaa acctgcacca atgatgaaca atggattgga gcaaatgggg atcgacctaa
    21061 atgaaactct aggtgctttg acaccgaaga aaaaagtgga acggacggta acagtcaaag
    21121 aagctttaga gttattgatc aaggaagaat cagcaaaatt agtcaaggat gctgatatcc
    21181 atagtgaggc aatcaaatta gcggaagaaa gtggaattat ttttatcgat gaaatcgata
    21241 agatcacttc taaatcacag caatctggag aagtatccag agaaggcgtc caaagagaca
    21301 tattgccaat tgtcgaaggt tcacaagtaa acacaaaata cgggatcatc caaacagatc
    21361 acgtcctatt cattgcttca ggagctttcc atttgtctaa accaagtgat ctaattccag
    21421 aacttcaagg acgcttcccg atacgggtag aattagatga tttgacagct gaagattttg
    21481 tgaagattct gaccgaacca aataatgctt tagtaaaaca atacgtcgcg ttgatcggta
    21541 cagaaaatgt gtcagttacg tttacaaaag aagcaattga acggatcgct caaatcgctt
    21601 tcgatgtcaa ccgtgacaca gataatatcg gcgcacgcag actgcatacc attttggaaa
    21661 aactactaga agatctatta tttgaagcac cagatatgca aatgggagaa ataacgatca
    21721 cagaagcata tgtggatgac aaacttggct caattgcggc aaatgaagat ttaagtcgct
    21781 atattttata aaaaatcgga ggacgacaat gacagacttg ctaacgaaaa cccgcaagat
    21841 aaacacactt ttgcagcaaa agaatacttt tgacatgcaa gcagatttgc cttacgataa
    21901 aatggctgtc acactaggtg gtattcttga cagcaacacc tatatcatag gaagtgacgg
    21961 agtattacta ggttataatg aacgtcacga tgtgaacaat gaacgaatca aaacaatgtt
    22021 tgttgagaaa caatttcctg aatcttatac tgatacagtg gcacagttga tgaaaactga
    22081 agctaatatc ccaattacta gtgatattac tgcatttccg gtagagtcaa gagagaaata
    22141 tccatttggt ctgacgactg tcgttccgat ctttggagct ggcgaacgtt tgggtacgat
    22201 tatcttatct cgtatggaac aatcgtttgc agatgacgat ctagtcttag cagaatatgg
    22261 agctacggtt gtcggtatgc agatccttta tcagaagtct cgaagcatcg aagcagatgt
    22321 acggagtgca acggctgtcc aaatggcaat caacacatta tcttatagcg aattgaaggc
    22381 tgtacaagct atctttgaag cattggatgg agatgaaggt cgtctgactg cttctaatat
    22441 tgccgatagt atcggcatca ctcgttctgt gatcgtcaat gctttacgaa aattggaatc
    22501 agctggaatc attgaatctc gttcgttagg catgaaggga acttatttaa aagtattgaa
    22561 ttcccgtttt aaagacgagt tagcaaaaca cagttattag aagtaagggg actggggcag
    22621 atagaaatgt ttgttccagc cttcccattt taaaaagtaa gtcgaagttg gaggaatggc
    22681 aatgactgtt attattcaaa atgaccagct gatcgccgaa atcgctgaac acggtgcgga
    22741 attgatcagt ctgaaatcaa aagaacatgg tttagagtat atctggcaag gtgatccagc
    22801 gtattggggc agacatgcac cggttttatt cccttttgtt ggacgtttaa aagatgatca
    22861 gtatgtttat caagggaaaa catatcaaat ggggcaacac ggatttgctc gggatatgga
    22921 ttttgaagtc attgaacatg gacaagaaat ggctagtttc atcttaagga gtacgccgga
    22981 gacattagaa aagtatccat ttgattttga attagtgatc agctatgaat taggaggaga
    23041 cggcatcacg gttcattatc aagtggcaaa taccggtgaa aaagaaatgt tcttttctat
    23101 cggtggacat cctgctttca atgtacctct tgaagaagga ttgtcatttg aagatttcta
    23161 tctggcattt tctccattga aatcaagatt acgcctacca ttgaaaggcc cattcatcga
    23221 tatggatcaa aagacgttag gtcagacaaa tacgaatctg gctttgaatc gtgaactttt
    23281 tgagcaagat gccttggttt ttgaaacaag gggtctaaat gcgtacgcca ttcgaagtga
    23341 aaaatcttcc catagcgtaa ctttaagcta taaagatatg ccttatgtag ggatctggtc
    23401 tccttatcca aaagaagcgc ctttcgtttg tatcgaaccg tggtgcggaa tcgctgacac
    23461 ggtgaacagt agtggaaact tgatggaaaa agtagggatc aatcaactag gtgcccatga
    23521 aatcttcaag acaaaatatt ccatcacagt gaagtaagag gttgtgaccg agaagttagc
    23581 agggaacacc gtttaccaag gtgtgtaaaa aagctatcct gtaagtaata agggagtgtg
    23641 acatcgattt
//
LOCUS       DACCDF010000040        22026 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCDF010000040 DACCDF010000000
VERSION     DACCDF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.041"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(61..1371)
                     /locus_tag="IVK51_001580"
     CDS             complement(61..1371)
                     /locus_tag="IVK51_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6431179.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IVK51_001581"
     CDS             complement(1383..2801)
                     /locus_tag="IVK51_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6431180.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IVK51_001582"
     CDS             complement(3041..4609)
                     /locus_tag="IVK51_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP6431181.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IVK51_001583"
     CDS             complement(4613..4894)
                     /locus_tag="IVK51_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6431182.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IVK51_001584"
     CDS             complement(4891..5814)
                     /locus_tag="IVK51_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP6431183.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IVK51_001585"
     CDS             complement(5811..6689)
                     /locus_tag="IVK51_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP6431184.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IVK51_001586"
     CDS             complement(6676..8013)
                     /locus_tag="IVK51_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP6431185.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IVK51_001587"
     CDS             complement(8016..9119)
                     /locus_tag="IVK51_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP6431186.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IVK51_001588"
     CDS             complement(9193..10701)
                     /locus_tag="IVK51_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6431187.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IVK51_001589"
     CDS             complement(10667..11359)
                     /locus_tag="IVK51_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6431188.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IVK51_001590"
     CDS             complement(11381..12355)
                     /locus_tag="IVK51_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6431189.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IVK51_001591"
     CDS             12655..13527
                     /locus_tag="IVK51_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6431190.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IVK51_001592"
     CDS             complement(14109..16307)
                     /locus_tag="IVK51_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6431191.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IVK51_001593"
     CDS             16539..17531
                     /locus_tag="IVK51_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6431192.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IVK51_001594"
     CDS             17591..19012
                     /locus_tag="IVK51_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP6431193.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IVK51_001595"
     CDS             19005..20504
                     /locus_tag="IVK51_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP6431194.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IVK51_001596"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IVK51_001596"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP6431195.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACCDF010000041        21942 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCDF010000041 DACCDF010000000
VERSION     DACCDF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.042"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IVK51_001597"
     CDS             complement(580..1596)
                     /gene="tsaD"
                     /locus_tag="IVK51_001597"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP6431196.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IVK51_001598"
     CDS             complement(1593..2057)
                     /gene="rimI"
                     /locus_tag="IVK51_001598"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6431197.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IVK51_001599"
     CDS             complement(2064..2606)
                     /gene="rimI"
                     /locus_tag="IVK51_001599"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP6431198.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LGVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IVK51_001600"
     CDS             complement(2590..3414)
                     /gene="tsaB"
                     /locus_tag="IVK51_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP6431199.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(3503..4483)
                     /locus_tag="IVK51_001601"
     CDS             complement(3503..4483)
                     /locus_tag="IVK51_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6431200.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(4507..5991)
                     /gene="galT"
                     /locus_tag="IVK51_001602"
     CDS             complement(4507..5991)
                     /gene="galT"
                     /locus_tag="IVK51_001602"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP6431201.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6003..6992)
                     /gene="galE"
                     /locus_tag="IVK51_001603"
     CDS             complement(6003..6992)
                     /gene="galE"
                     /locus_tag="IVK51_001603"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP6431202.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7240..7407
                     /locus_tag="IVK51_001604"
     CDS             7240..7407
                     /locus_tag="IVK51_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP6431203.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(7469..9280)
                     /locus_tag="IVK51_001605"
     CDS             complement(7469..9280)
                     /locus_tag="IVK51_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP6431204.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(9277..9642)
                     /locus_tag="IVK51_001606"
     CDS             complement(9277..9642)
                     /locus_tag="IVK51_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP6431205.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(9805..10200)
                     /locus_tag="IVK51_001607"
     CDS             complement(9805..10200)
                     /locus_tag="IVK51_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6431206.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10218..11180)
                     /locus_tag="IVK51_001608"
     CDS             complement(10218..11180)
                     /locus_tag="IVK51_001608"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP6431207.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11180..11392)
                     /locus_tag="IVK51_001609"
     CDS             complement(11180..11392)
                     /locus_tag="IVK51_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP6431208.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(11413..12111)
                     /locus_tag="IVK51_001610"
     CDS             complement(11413..12111)
                     /locus_tag="IVK51_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP6431209.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12131..12673)
                     /locus_tag="IVK51_001611"
     CDS             complement(12131..12673)
                     /locus_tag="IVK51_001611"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP6431210.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(12805..13809)
                     /locus_tag="IVK51_001612"
     CDS             complement(12805..13809)
                     /locus_tag="IVK51_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6431211.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(13806..14795)
                     /locus_tag="IVK51_001613"
     CDS             complement(13806..14795)
                     /locus_tag="IVK51_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP6431212.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(14792..15598)
                     /locus_tag="IVK51_001614"
     CDS             complement(14792..15598)
                     /locus_tag="IVK51_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431213.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            15764..16720
                     /locus_tag="IVK51_001615"
     CDS             15764..16720
                     /locus_tag="IVK51_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6431214.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVRKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(16797..17315)
                     /locus_tag="IVK51_001616"
     CDS             complement(16797..17315)
                     /locus_tag="IVK51_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6431215.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="IVK51_001617"
     CDS             complement(17403..17552)
                     /gene="rpmG"
                     /locus_tag="IVK51_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP6431216.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            17780..18226
                     /locus_tag="IVK51_001618"
     CDS             17780..18226
                     /locus_tag="IVK51_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6431217.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="IVK51_001619"
     CDS             complement(18421..20316)
                     /gene="asnB"
                     /locus_tag="IVK51_001619"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP6431218.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(20641..21615)
                     /locus_tag="IVK51_001620"
     CDS             complement(20641..21615)
                     /locus_tag="IVK51_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6431219.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT         6583 a         4655 c         3757 g         6947 t
ORIGIN      
        1 aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc ggcagtttta
       61 gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa tattcactgt
      121 ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc gatctagatt
      181 attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg aacggataat
      241 gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat gtaaaaaata
      301 gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta cctgtcaact
      361 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
      421 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
      481 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
      541 tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata aaaccaagct
      601 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
      661 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
      721 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
      781 tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
      841 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
      901 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
      961 aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt aattatcttt
     1021 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
     1081 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
     1141 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
     1201 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
     1261 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
     1321 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
     1381 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
     1441 gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta tttgagaagc
     1501 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
     1561 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
     1621 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
     1681 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
     1741 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
     1801 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
     1861 attactttcg agtatccaat aaagccacag atctggttat cttcagctaa aagcagataa
     1921 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
     1981 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
     2041 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
     2101 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa ccccaatttc
     2161 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
     2221 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
     2281 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
     2341 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
     2401 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
     2461 cgttcgacac ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
     2521 cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
     2581 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
     2641 tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
     2701 tggttcaact aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
     2761 aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga atttgtgaca
     2821 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
     2881 agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc agcggtatgc
     2941 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat tatccacacc
     3001 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
     3061 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
     3121 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
     3181 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
     3241 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
     3301 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
     3361 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat acatgattat
     3421 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcactccat
     3481 tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
     3541 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
     3601 ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc gttacatatt
     3661 tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc actaaacctt
     3721 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
     3781 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
     3841 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
     3901 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
     3961 caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact ttttgaacac
     4021 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
     4081 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
     4141 gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct ttcaataaag
     4201 taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa taataaagat
     4261 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
     4321 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
     4381 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
     4441 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
     4501 ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
     4561 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
     4621 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
     4681 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
     4741 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
     4801 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
     4861 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
     4921 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
     4981 ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa taagcaagtt
     5041 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
     5101 ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
     5161 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa ttggtaagtc
     5221 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
     5281 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
     5341 gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat tcattcgaat
     5401 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
     5461 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
     5521 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
     5581 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
     5641 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
     5701 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
     5761 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
     5821 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
     5881 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
     5941 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
     6001 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatc
     6061 gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt ttcacttgaa
     6121 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
     6181 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
     6241 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
     6301 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
     6361 tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa aatgattggg
     6421 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
     6481 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
     6541 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
     6601 tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga gaatacgatt
     6661 ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
     6721 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
     6781 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
     6841 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
     6901 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
     6961 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
     7021 gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg aaaacaaaca
     7081 aagtaaaatt tcgtaaattc atatggtaaa tttataataa aacatttagt aaacattttc
     7141 aaggattttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt
     7201 ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt tgtatctgga
     7261 atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc ttctgtgaaa
     7321 aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa
     7381 gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga agactttcta
     7441 aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt tgtgctattt
     7501 tatctgcctg tctcagattc catggatctg tataaggtct agctactgct gcagttagcc
     7561 aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt tcccctgaag
     7621 catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga taacttcctg
     7681 tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt tttatgacag
     7741 gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc
     7801 tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga atatcaaatg
     7861 gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct ttcgtccgaa
     7921 tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc catagtattt
     7981 ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc
     8041 agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct ttttccgctt
     8101 cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca ttctgatagg
     8161 caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct ggatgctttc
     8221 cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc tttggaatcg
     8281 gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct tttttcagat
     8341 agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc aggttttctt
     8401 ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt cctcgagggt
     8461 aataagggag cccccttctt gatccactgt atacaatcgg ttccttacct gatggatgat
     8521 agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta aacaatccga
     8581 cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta ggcggtatct
     8641 tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca tttttctttg
     8701 cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga ccgcttgcta
     8761 aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa ccatcttttc
     8821 ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga aattcttctt
     8881 gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta gattgctgat
     8941 ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt accgtctgtt
     9001 cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc tgtgttacca
     9061 atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa acagaattca
     9121 ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca ttgggatcga
     9181 aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt tctgctacac
     9241 ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct ccatttcgat
     9301 aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat aaacaaaagt
     9361 aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct tcttttcttc
     9421 ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg
     9481 gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca gatctaactg
     9541 cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc tatagccatc
     9601 ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt ccttctttct
     9661 accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa tgaacggcac
     9721 aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga ttcggttttt
     9781 aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc cagaccatcc
     9841 gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta atttatttgc
     9901 tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt cttttcgaat
     9961 agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta aaatatgccc
    10021 agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca ttccttctcg
    10081 aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta gatagacttc
    10141 ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc ataaaaccat
    10201 gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc aatgacacca
    10261 gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg cgggaaagta
    10321 aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc cgatactcct
    10381 ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca agctaaacct
    10441 aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt
    10501 tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt cagttttgaa
    10561 tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt agcgctagat
    10621 acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg atccacagtg
    10681 atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc gccagcaacg
    10741 atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc gttttctcca
    10801 gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat agcgaatttg
    10861 attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca gtttaagtta
    10921 tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc catgccgatt
    10981 ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat tgattcaatg
    11041 atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct ccatttttgc
    11101 tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc taaatcaatc
    11161 ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca attcggtgct
    11221 ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa cggctatcat
    11281 acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca cccacataga
    11341 taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc atctttaagc
    11401 acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa tacatattac
    11461 aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg
    11521 ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc caccgacatg
    11581 tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca caataaataa
    11641 aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa accctaagat
    11701 aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca gaacattcgc
    11761 ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat aaatcaagaa
    11821 aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca gtatcagtga
    11881 gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta aacgccacca
    11941 ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga tctcaagtcg
    12001 cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca
    12061 taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca tgaaaacctc
    12121 ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc tccggttgcc
    12181 attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga aaattattta
    12241 aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta ttgtagataa
    12301 tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct gattgtggct
    12361 caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca taaaacgcca
    12421 tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt tctgctcgtt
    12481 ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct atcacttttg
    12541 ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct ttttcctttt
    12601 tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt aatcttactt
    12661 tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga aaaaaagaaa
    12721 aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac ctagaaatga
    12781 ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc tgataaaaag
    12841 tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag gtacttcccc
    12901 acttagtaga atcactctag caaccatatc tgcactaagc agaaaaatgg aaccgaacaa
    12961 accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg cagcatgagg
    13021 agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc ttgccaaagc
    13081 aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta atccttgcgc
    13141 gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc ctagcggaat
    13201 taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac cccagattga
    13261 tcccgctaac catgcattca caaatccata attttcctta gataattgga tcgtgcatag
    13321 caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga ttcgattcat
    13381 acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag ctaaaacacc
    13441 acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa
    13501 ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag aatctgccaa
    13561 ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag ctcctgctaa
    13621 taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca aagaagatga
    13681 ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg taccaatcat
    13741 taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg tccaatatat
    13801 ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc aatcaatgca
    13861 atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc
    13921 aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg atagtccgtt
    13981 ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac aggtccgact
    14041 aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa ccgcgttcgt
    14101 ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa agatattgct
    14161 tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa tactgctaat
    14221 tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc ctgctgcaga
    14281 ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat agctacgcca
    14341 gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa accaaatacg
    14401 agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa atcagaagct
    14461 ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt gattcctagt
    14521 aagcctgaat ctgctaaagg atttcttgtc actccttgca tcaatgcacc gctcaatgca
    14581 aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt tcgtatgatt
    14641 tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc tgcttttccc
    14701 acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc
    14761 aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc tcttttggca
    14821 acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca atcgttgcat
    14881 caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt gtaccttctg
    14941 tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa gcatgattaa
    15001 tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca gtcaataact
    15061 gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc aagatcaaga
    15121 tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt ccaccagaca
    15181 gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac gcccactcaa
    15241 ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg tatcgtccgt
    15301 aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt gctaataatg
    15361 caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta ttgacaatga
    15421 tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta gattttccac
    15481 ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca gacatgtttt
    15541 caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat ctcgtcattt
    15601 cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca tttcgtcaag
    15661 cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat atcaggaata
    15721 ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa
    15781 agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt gctccccctc
    15841 ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata gtactcatac
    15901 agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac ggatcatcgg
    15961 cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc agtggactgt
    16021 tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc caacgatttc
    16081 ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt tgatttcttc
    16141 ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc caacttatgt
    16201 tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg gcaaagcttt
    16261 ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca tcaagaaaac
    16321 tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt gggtgaccaa
    16381 caactcagcc tttatggtca gaaaaaatcg gtcaagtgga agaatcgtat acgacgatct
    16441 ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt ctgactggtc
    16501 tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc ttgtgaacag
    16561 tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag cagtgacaga
    16621 caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac ctattgccag
    16681 tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa aaaaagcatc
    16741 ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca tagattttat
    16801 tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg atgggcatat
    16861 agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc tgaaaaatca
    16921 atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg ccagatccga
    16981 ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata gatatcttct
    17041 gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc cgcattttct
    17101 ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg aagtgaaaga
    17161 tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat tgtgtgttcg
    17221 cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt gccgatcaga
    17281 ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta tgattttaga
    17341 tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga actttaaaaa
    17401 cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata tttttgtttt
    17461 tccaaacgat caggattgtt acgtttattt ttgcttgtta gatagttacg ttctttacaa
    17521 gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt tacaagaatc
    17581 ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat tgctagtgtt
    17641 tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt acttctcaaa
    17701 caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat tatgtttttt
    17761 aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat caatgaaaaa
    17821 cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat tagatgaagg
    17881 gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga
    17941 tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca agacggatga
    18001 acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg tagaatatga
    18061 ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc attttgatac
    18121 aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat caactttgtt
    18181 catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat atgtaaaggg
    18241 gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt tcctctgcca
    18301 actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt gaaattgttt
    18361 cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat ttgattttca
    18421 tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta caaagtagac
    18481 tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt ctttacgtcc
    18541 ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt cttttgcaaa
    18601 atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca accaagcttt
    18661 gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa gatgtttgtt
    18721 cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact tacttggtat
    18781 ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt ccaaagaact
    18841 tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt gatgaagatc
    18901 gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt aatgaacagt
    18961 catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt ctgatgcttc
    19021 tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag caagtgattc
    19081 atataaatgg atccgtccat ggaaattttt catttttccg attttacgtc ctaaattata
    19141 gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga attttgaatt
    19201 cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt ctccagattg
    19261 gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg
    19321 atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga acgacatgtc
    19381 tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt ttctggcttc
    19441 aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat ctggtttcaa
    19501 tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa cttctacatc
    19561 actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt cgatccattc
    19621 ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct gaatgtctat
    19681 ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat ctttgaagaa
    19741 tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg cttctttgtt
    19801 caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac caaacataaa
    19861 tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc ttgcaccgaa
    19921 taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga tcttttgcaa
    19981 taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg catgtgtttg
    20041 aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat agatttcgcc
    20101 attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac cgccctctaa
    20161 gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat attctcctga
    20221 actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg tttttttgtt
    20281 gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc ctaatataaa
    20341 attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga cgaatatttt
    20401 ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat
    20461 aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat tctcttcgtg
    20521 ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga cacgacctaa
    20581 agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc tattcattaa
    20641 ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca attcatgacg
    20701 atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat acgtacgata
    20761 ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt cgtattttct
    20821 ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat gaaaaaattg
    20881 actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa atgtggcttt
    20941 tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca tcccacgact
    21001 ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttccat ttgatagcga
    21061 gcgataattg ttcagattaa ataattgata atcaaatggc ggattatttg tcaacacacc
    21121 gacaggatta tcatataaat gaagtccatc tttcatactt tcaattacta tcgatttttc
    21181 tttatcagct aataaccagt gtaaaggtga caagggtagt ttttcactaa aatcgatatt
    21241 gactagattg atagtagcca ataattcttt agcttcgtct atggttgagc attgccctag
    21301 tatccatgga ataaactcaa atggtgcaac gttatctttt ccttcttgta attctttata
    21361 atcagcattg ccaggaaaat tcagtcctgc catactcaac cctttttcat ttgtagcctc
    21421 ataatagagt ggatagttgg ataccccagc tgctattcct atcattgcat aatgtttatc
    21481 taaatttttt acctttctaa aatcgaaacg ataatttctt ggagtaatag tgaccacttc
    21541 attataagac atctcataat caaaattcct tccaaaataa tggtctttcg ttgcataagt
    21601 gattgcggta cacatcgttt cccgtcctaa aacaaatcgt ttttacactt ttatcttaca
    21661 tctagtattt tttttatcaa aaaaaggcca aagaagattg atttactagc atttataaaa
    21721 aatagtactc agggggaatc aaaaaatatt gtaggttaaa agtacaacaa taaggggctg
    21781 tgacaaaagt cttgtcacag ccccttattc cgaacaaacg gtgttcaaaa gcttgcatct
    21841 gcggtaataa gcccaatcaa acgaaaaaat aggaaaagct atttttgttt ggccgttctt
    21901 attacttgat gcaggacagc ttcttcacaa cctctaatcg tt
//
LOCUS       DACCDF010000042        21658 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCDF010000042 DACCDF010000000
VERSION     DACCDF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.043"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(412..1908)
                     /gene="lysS"
                     /locus_tag="IVK51_001621"
     CDS             complement(412..1908)
                     /gene="lysS"
                     /locus_tag="IVK51_001621"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP6431220.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2019..3023)
                     /gene="dusB"
                     /locus_tag="IVK51_001622"
     CDS             complement(2019..3023)
                     /gene="dusB"
                     /locus_tag="IVK51_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP6431221.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3024..3911)
                     /gene="hslO"
                     /locus_tag="IVK51_001623"
     CDS             complement(3024..3911)
                     /gene="hslO"
                     /locus_tag="IVK51_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP6431222.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4128..6239)
                     /gene="hflB"
                     /locus_tag="IVK51_001624"
     CDS             complement(4128..6239)
                     /gene="hflB"
                     /locus_tag="IVK51_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP6431223.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6329..6874)
                     /gene="hpt"
                     /locus_tag="IVK51_001625"
     CDS             complement(6329..6874)
                     /gene="hpt"
                     /locus_tag="IVK51_001625"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP6431224.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6874..8319)
                     /gene="tilS"
                     /locus_tag="IVK51_001626"
     CDS             complement(6874..8319)
                     /gene="tilS"
                     /locus_tag="IVK51_001626"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP6431225.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8439..8909)
                     /locus_tag="IVK51_001627"
     CDS             complement(8439..8909)
                     /locus_tag="IVK51_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP6431226.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8955..9380)
                     /locus_tag="IVK51_001628"
     CDS             complement(8955..9380)
                     /locus_tag="IVK51_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP6431227.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9447..9716)
                     /locus_tag="IVK51_001629"
     CDS             complement(9447..9716)
                     /locus_tag="IVK51_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP6431228.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9727..11322)
                     /locus_tag="IVK51_001630"
     CDS             complement(9727..11322)
                     /locus_tag="IVK51_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6431229.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11337..14858)
                     /gene="mfd"
                     /locus_tag="IVK51_001631"
     CDS             complement(11337..14858)
                     /gene="mfd"
                     /locus_tag="IVK51_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP6431230.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14875..15435)
                     /locus_tag="IVK51_001632"
     CDS             complement(14875..15435)
                     /locus_tag="IVK51_001632"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP6431231.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15788..16762
                     /locus_tag="IVK51_001633"
     CDS             15788..16762
                     /locus_tag="IVK51_001633"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP6431232.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16932..18332
                     /locus_tag="IVK51_001634"
     CDS             16932..18332
                     /locus_tag="IVK51_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP6431233.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18410..19132)
                     /locus_tag="IVK51_001635"
     CDS             complement(18410..19132)
                     /locus_tag="IVK51_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP6431234.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19211..19717)
                     /locus_tag="IVK51_001636"
     CDS             complement(19211..19717)
                     /locus_tag="IVK51_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP6431235.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19840..21639
                     /locus_tag="IVK51_001637"
     CDS             19840..21639
                     /locus_tag="IVK51_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP6431236.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WEEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6011 a         4726 c         3884 g         7037 t
ORIGIN      
        1 gcaactcatt tatcataact tatcgtttgt tatttgtcaa gaactttttt gaagtttttt
       61 tcgatagaat caagcttcgc aagtggttct tttttaacga ctcagttatc ttatcatgtc
      121 gttagtaaca tgtcaataac ttttttgtta tgtttttgaa taaatatttt cttctgtctt
      181 ttggacaagt aataatatac caactaaatt tagcttggtc aacgaatttt ttgtattttt
      241 tcaagttttt ttcaataaac gattattcat aacttttctt tctttatcgt tcgttttcgt
      301 cttctttaat gtctagaata gtcggcatta gcttttgaac tttcgttatt atttattcct
      361 ttctacacag caaaagctgc ctaaggaatt ccttagacag cttttattcg attatctcat
      421 tgttgggaat agcaaaacat cccgaattga ttgtgcatca gttagcaaca ttactaatct
      481 gtcgatcccg atgcctagac caccagttgg cggcataccg tattctaagg cttccaagaa
      541 gtcttcgtct acaccgtgag cttcgtcatt tcctagttca cgttcttttt cttgcgcttc
      601 gaatctttct ctttgatcaa ttggatcgtt cagttctgta aaggcattag caaattcgcg
      661 ccccactatg aatagttcga atcgatctgt gaatcgagga tcttctggat ttttcttcgc
      721 taaaggcgaa acttcgactg gatgtccgta tacaaacgtc ggttgtgtca atgtttcttc
      781 aacaaatgtt tcaaagaatt cattgataac atgtccgaca cccatagctt cggttacttc
      841 tacattgtgt ttttcggcta aagctaatgc ttcttccaat gtcatttctt gccagaagtc
      901 tacaccagtt tgttctttga ttgcatctac catatggatg cgtttaaagt cactttctaa
      961 gtcgacagct tgtccatcat atgtgatttt tgctgttcct agtacttttt cagctgcgtt
     1021 acggataata ccttctgtca gattcattac atcattgaaa tcagtatatg ctgtgtatgc
     1081 ttctaacatc gtaaattctg gattatgtgt agtatcaatt ccttcattac ggaaaacgcg
     1141 gccgatttca taaacttttt ccattccgcc aacaattagt cgttttaaat gaagttctag
     1201 cgcaatacgc aaatataaat caatgtccag tgcattatga tgcgtgataa atgggcgtgc
     1261 ggcagctcca cctgcctcat tgtgcaatac tggtgtttct acttcaatat agccattgcc
     1321 atctaaatag cgacggattt cgctgataat ttggcttcgt ttcataaatc ggtcaaagct
     1381 ttcgcgatta ctgattaaat ccaagtagcg ttgtctgtag cgttgttcaa tattcgttaa
     1441 gccatggtat ttatctggca gtggacgaag tgcttttgac aaaatcgtga tttctgaagc
     1501 tttgatagaa acttcgcctg tgtctgtttt catgatttgt ccagttacac caaagaaatc
     1561 tccaaggtct gcatgtttga atacttcata ggcctcatca cctacttggt cttttcttac
     1621 ataaatttga atttggcctt cacgatcttg tagatgcgca aatccagctt tcccttttcc
     1681 tcggcgagtc atcatacgac cagctacact agcggtcaat cccatttctg ctagttcttc
     1741 ttttgttctt ggatcaaata gttcatgcaa ttcttctgag ttgtgcgtgc gttcaaatcg
     1801 ttttccaaat ggatcgattc catcttcacg caattgttcc attttttgac gacgtacaag
     1861 catttgatca tttaaatctt ctacttgatg ttgttgttcc tgtgtcacaa aagttcctcc
     1921 attctcatgt taatctcttt cctataatgc caatgaaaca caaaaaaagc aagccgaact
     1981 tggaatttcg gcttgaaaat tcacgaaaaa cttagaaatc acaacgtttg tttcaatagt
     2041 actttttccg ctttttctac aaattgatcc agcaggtcat tgatttcttg ctgtttttct
     2101 gtctgattga ttgctacttt cactttcgct gctcgaggaa tcccttttaa ataataagcc
     2161 gcatgtgttc tgaattctct acaagctact ttttctcctt tgagatctgc taagcgttgt
     2221 aaatgaagtt tagctgtagc aattttttcg gctggtgttg gttcaggaat cagttctcct
     2281 gtttcgagat aatgctgtgt gcggtgaatc atccatggat tgcccagcgc tgcacgacca
     2341 atcatcacac cgtctgcacc gacatagtca agcatccatt ttgcatcttc tggcgttttg
     2401 acatccccgt tccccataaa tgggatcgtc aaatgttttt tcacttcttt caatacttcc
     2461 cagtccgctt ttccttcata catctgaacc cgtgtacgtc catgcatagc gatggcactt
     2521 gctccagctg attgtgctgc taatgcattt tctacagcaa agacgtgctc ttcatcccaa
     2581 ccaatacgca tcttgattgt tactgggata tttactgctg aagaaacagc ttggaccatt
     2641 tcgtatactt tctcaggatc taaaagccat tttgctcctg cttctgcttt gattacttta
     2701 tttactggac agcccatatt gatatctatt atatcggcag ctgtgttttc ttcgacaaat
     2761 tgagcagctt caactagact atctttattt ccgccaaata tctgtaaact taatggatgt
     2821 tctgtttctt caatgtggag catctctaaa gtttttttgt ttctgaaatg aataccttgg
     2881 tcactgatca tttcacatac gactaagccc gcaccaaatt ctttgacagt gactcgaaat
     2941 gctgaatttg ttattccagc catcggtgca acaacgacac gattagggat ttccacattg
     3001 ccaattttcc atgtgtgttc cacttaaatc cctcctaagg cttctttctt caatccatct
     3061 aattcttctt cgctatactt gtatttgtta ttacagaaag aacaaaccgc ttctgcaccg
     3121 tgatcttcgt ctatcattgc ttggatttct tctgcaccta acgcgataat tgcttctgca
     3181 aatttttctt ttgaacagtc acatttgaat tgaacaggca ttttttccaa aatgttcaat
     3241 tcttctgtac caagcaatcc tttcaatatt tcttctggtg tctgtccttc atctaagagg
     3301 gtagagattc ttggtgtcgt ttttaattgt tcttcgatct tgctgattgt ttcttcatcg
     3361 gcacctggca tgatttggat catgaatcct cccgccgtct tgatgctgtc gtcagtattg
     3421 accaatacac tcagtccaac agctgaaggg atttgttctg agatagcaag atagtaagta
     3481 aaatcttcag cgatttctcc agaaacaatc ggcgtttgcc cagaaaaagg ttcttttaat
     3541 cctaagtctt ttgtgacagt aaacattcct tgtgttccaa ctgctccccg cacatcgatt
     3601 ttaccttcac tatttagagg aagactgaga tgcggatttt tgatgtaacc tttcacatct
     3661 ccctttccat ttgcatctac tacaattcct gttgctggac catcgccttg gattcgaaca
     3721 gttagtttgt cttctccttt gagcgtcgca ccaagtagca gactaccgac caatgtccgt
     3781 cccaaagcag ctgaggaggt gtgccaagta tcatgacgtc tttgtgcctc cgctacggta
     3841 tttgttgctt gaacggcgta tgctcggacg aatccgtcgt gagcaagtgc cttaaccaaa
     3901 taatctttca tttgttatgt actccttttt ccgtttttaa actcatagtg aatcatacta
     3961 gcaactactg cttttttcaa ctttggaatg cttcttttaa aaataattca gaatcatcta
     4021 gaaaacagga catcacccta tttacaactt aaataaagaa atctcccgaa aaccggtttt
     4081 tgttccggtt ttcgggagtc atatcagatc aattgaccta tattttttta tttgtattga
     4141 tcgtctgaat gattatcttc tgattgttga gaatctgaat catgttctgc tttattttcc
     4201 tcattcagtt catgttttgc ttcttcaaaa tcacgttttt cttccgcttg tttttcagcg
     4261 tctttttctt ccaaagcacg ttttgcttct tcaaaagttt tcgcttcttt ttcgcttgga
     4321 taatcatttg actcagtacc ttcaggcatc acaccgtgtt caaacaatga cttgattgct
     4381 ttggcatcca atgtttcgaa ttccaaaagt ttttcagcaa tcagtttatg ctgtgcacga
     4441 tgagcttcga tgatttctcg agctttatca tgagcttcca tcaagatttt tcgtacttct
     4501 tgatcaatct cgaaagcgac ttgttccgag taagcttttg tttgaccata gtcgcggcca
     4561 acaaacactt gatggtttcc ttcatattga acaggaccta aacgatcact cattccatat
     4621 tctgttacca tgcttcgtgc tagagctgtc gcttgctcaa agtcattgga agcaccagtt
     4681 gattggacat tgaagatgat ttcttcagct gtacgtccac caagtaagcc tacgatttgt
     4741 tcaaacatat cttctttcgt catcaagaac tgatcttctt ttggtaaagc gatcatatat
     4801 ccgccggcgc gtccgcgagg gatgatcgtt actttgtgga cgacccgagc gcgactcaat
     4861 actaatccga caatcgtatg accagcttcg tgataagcaa ccatttcgcg ttcttttttg
     4921 ctgattacgc gatctttttt agctggacct gcaatcacgc gatcttccgc ttcatcgata
     4981 tcagaagcat cgattttctt tttatttctt ctagcagcta ctaacgctgc ttcattcaag
     5041 acattttcta aatctgctcc ggcaaaacct ggtgtctgtt gagccacaac tttcaagtca
     5101 acgtcatctg ccatcggttt attccgtgca tggactcgca agattgcttc acgacctttt
     5161 acatctggac gtccaactaa aatctggcgg tcaaaacggc ctggacgtag taacgctggg
     5221 tctagtacat cagaacggtt ggttgcagca atgacgatta ctccttcatt tccgtcgaag
     5281 ccatccattt ctacaagcaa ttggttcaat gtttgttcgc gttcatcatg tccgccgccc
     5341 ataccggcac cacgttgacg acctaccgca tcaatttcat caatgaaaat gatagctggt
     5401 gcgtttttct tcgctgtttc aaacaaatca cgaacacgac ttgctccaac accgacaaac
     5461 atttctacga aatcagaacc cgagatcgag taaaacggta ctccagcttc tccggcaaca
     5521 gcttttgcta ataaggtttt acctgttcct ggaggtcctt ctaaaagtac acctgccggg
     5581 atacgggcac ctaattcaac aaatcggcga ggatctttca gaaattcaac aacttcaact
     5641 agttcttgtt tttcttcttc cgcacccgct acatcagaaa aacgtacgcg attagctttt
     5701 ttgtctgctt ctttggcttt tgatttacca aagttcatga cacggccgcc accgccaccg
     5761 ccgccgcctt gttgtcccat catcatgtaa aagaaaaaga tgatgattac gattggcaag
     5821 aagctaagca atagggaaac ccatataccg ctgcttgatt cttcttttac tgttgtagca
     5881 actttgtttt cttttgccag ttctgttact tggctcaatg ttgtatcatt tggcaaaatg
     5941 attgttgtga aatgggtagt agtcgtatct gttgaaccta agatagctaa cccaccgctg
     6001 ttagcgacgg tctgctgttc tttgtattca ccagtgatct tatatacacc atttgcaggc
     6061 tgcacttcaa attctttgat cttgcctgct tgtagttgtt ctgtgaattt tgagtactcg
     6121 atatctgacg atcgctggcc gttattacca aaaatgaaat aaacgaccat taccatcgct
     6181 aagattacga ggacataata caaggcattt ttcatcatgc cgttgttctt tttgttcata
     6241 gttgccctcc ctaagcgtca atttaattta acagtttttt atcaatcctg accattctat
     6301 tgtaccataa tttaattgag atacagcatt aatttgattg ataaacacta ggtttcaata
     6361 caccgatgta cggaaggtta cgatatgctt ctgcataatc caagccataa ccgaccacaa
     6421 attcatttgg tacgttgaag ccaacataat ctgcttcgat atttactact cttccttcag
     6481 gtttgtcaag tagtgtaaca attttgactg attttgcttt gcggtatttg aaaagatcca
     6541 ctaaataagc aagtgttctt ccgctgtcaa tgatatcttc aacgatcaac agatcgcgac
     6601 cttctacatt cgtatctaag tctttgacga tttttacttc ccctgacgag actgttgcat
     6661 tcccgtagct tgaaacatcc atgaaatcca attccaaata cgtatcgata gaacgaacga
     6721 tatctgccat gaatggtacc gctcctttta agacacctac tactagggga ttggtgtttt
     6781 ggtaaatttc tgttaactct ttgcctagtt ctttgcaacg aacttgtatc tcttcttgtg
     6841 agatcaaaat tctctcaata tccttttcca acatcaggtt ctccttccaa ttgcttcttt
     6901 ttgatatttg taaagaagta tgtagtgtat tttatcagtt tcttcacgaa tactcaaata
     6961 tgaaaaggca aaaggtatca agctatatat atttcttttg gcatctgtaa tgatccaagc
     7021 atgctgcctt tgtgaattag gtactttctg atcgataaaa tatctagata ctttttttgt
     7081 gagattctct gttaatttaa tgcggtctcc tgcttttctt ttttcgacat acagttgcac
     7141 atcagcagga atccaaatgt catgagaaaa tagagaccat gaatctgttg ctttgatgtc
     7201 ccctgcattt tcaggtttga ccagaccaat ccattctgtt tcattcaaat atattccttg
     7261 ttctggaatc agcaacaagc gctggtcttc tgtctggcta gttgcttgtt tttctttttt
     7321 agagagtaca acaaaatgat actctctttt cagtatccaa ttattgccaa tactgagttg
     7381 ccattggctt tttttctgtt gccactggtc taaaatcgac tccatttgtt gttctttcaa
     7441 aataattcct gtttttgaga atgctttgtc gaaaaaggag taaaaaaaat agtatcgttc
     7501 agcatctgtc atttcttcaa tcatttctgc agaaaatcga aattgttcct cccactgttc
     7561 gacttccttt tcgatcaact gtcccatata tttttgaatg acttggtctg cccattggat
     7621 ctgttgcgaa aattgctgga aatgtctcaa cgcctgtgta ttttcttgtt tcaattgagg
     7681 aactaccaaa tgacgaagcc gatttctctg gatatctaac aattggtttg tctgatcctc
     7741 aaagtaaaca agctgcgatt ccgctgcata agtatatagg tcttctttgc taaaactcag
     7801 caacggacga atcagtctgc ccgtagcaaa tggttgtact tctttgattc cagaaaaagt
     7861 atttaactgt cccccacgaa cgattttcat taacaccgtc tcgatttgat catctccgtg
     7921 atgagcagtc atcaacgtgt catattgtcc ttcttccatc acgcgagtga attcttgata
     7981 acgaaagtta cgagcttctg tttctatggc ggatttggct ggatcttccc aaatacttac
     8041 atataaaggg agattcttct cttcacaata tgagcgaaga taggcagctt cttctgctga
     8101 ttcttctctt aattggtgat tgatgtggac gatacctaaa gaaaaaccga tttggcgttg
     8161 cacttgtaaa agtaaatgga gcaaaaccat cgaatcaaca ccacctgata ccgctgctaa
     8221 aattctcgat ccttgttgcc agaatccttg ttcattcccg tgccgaataa atgactgctg
     8281 taatagcggt gcttttttcc cgcctttttc aacgttcatc ctttccacac cctttcttcc
     8341 taaaaaaaag agagcgggaa aacgtctaaa acgacaatta acctgccctc tgttgatact
     8401 atgacccaaa tagtcgcact atcattattt gcggcttttt agctgcgacg tccgccacgg
     8461 ccaccgcgct ttccttctgt gtttcgcttc aatgaagata agcggtcatc gctatctttc
     8521 aaaaatgaac tcatcaatga atcgaagtct tctttttgag ctgaaggttg actgcgagaa
     8581 aaagattttt tctgtgctgg acgatttcgg ttgtcacggg gatctttttg ttgatatttt
     8641 ttaggacggc tttcttctcc agattcagca ggttgttcct gcgctttccg gatagatagt
     8701 ccgatttttc catcatcatt tactgaaata actttaactg ttacttcatc tcctacactt
     8761 aaaacatcat ggatgtcttt tacatagcca tttgacactt cgctaatatg aactagaccc
     8821 gtttttccag cacctagatc aataaacgca ccaaaatttg tgattcctga tacttttcct
     8881 tgcagttttg ctcctacctc gattgacata aaaaaaatgt tcctcctaat tatttccctt
     8941 ttttatattc ctgcttattt tgcagttgta gaactagttg attgatctgt ttcatttgtc
     9001 gagctgtctg tatttgattc taatatagga taaacttttt caccgtcttt tgagtaaaag
     9061 aatttgcttc tggcaagttt agctacatac tcatcatctt tcagcaatga aacatcttgg
     9121 tgcagctgat caacgtctgc gttgacttct ttcatttcag tatttgcttc gactctcaat
     9181 tcgttcaatt gatgcatcct ttgcatgtca tggaaaatct gcacacctat tatcgcgaaa
     9241 atgaccaaag caccaacaaa aattgcagcc agtctgcgtc ttcgaaaaat cagttgacgt
     9301 tgttgttgct gaaattcagc atactttttc tttgcatatt ccgtatctag cgcagcgatt
     9361 ttattgcttt ttttcgtcac ttggattcgc tccaatcttt ttgattgtct ttttcattat
     9421 accaatatcg tgaaagcttg tctaatctat ttctctagtg tttctgtgcg attttcagaa
     9481 ataatttcgt acattttttg tgcatcttct tttttggttg attcatgtaa ttccgtaatt
     9541 ttcacttcca acgttttgtt gccaaactgg actttcaaaa tatcgcctac tttgacatca
     9601 ctggaagatt tcgccaattt accgttgatt tgaattcttc ctttatcagc tacttctttt
     9661 gctactgatc gtcttttgat gattcgggaa atttttaaaa atttatctaa tcgcatatct
     9721 tattcctcac tttttaattt ttttatttaa tcgcaggatt ttcttaccaa aaggcaacat
     9781 tagccattct cgtactgtaa acagacgaag acgaatcact gcataaatga acacagaagc
     9841 cccgatccca actcctatca cgctgacaaa aaaagcagtg gaacgatgag ttacaggacc
     9901 aaatactata ttcaatacac cataatacag cagcagtact actgtcatac aaagaaggct
     9961 gaacatcaac ttccgtccaa aatgccgttc tttccaaaaa gcatttagct cttttgtttc
    10021 aattcgaaca aaataccaaa gcgtcacacc taatcctaaa agggtagaca ggcttgcacc
    10081 tgctgttcct aataaaccgg tcagaaaaga agtagtcaat ccttttacca atagtcccca
    10141 tcctgcgcct tttaaagaag ggcgaaacga gttcttgctt tgagcaatac tttgataagc
    10201 ttggatgact gcagtgaaag caatggaaaa aacaaataat acaagcgctg catttcccgc
    10261 ataatcttta aacaacgcat aattgatata agggagcagc aaggctaatc ccaaagacgc
    10321 agctaatgct aaagcggtcg tcaatctaag atagattttg gctgtctgta aaaactgacg
    10381 atagatccgg ttggtcaaat gacgagtcaa cgcaggcagg aatgtcgcac ttaatgcagt
    10441 ggcaatcact aaacctaatt gtactaaagg ctgacctcga tcatagaccc cttttgctat
    10501 cttcgcaccg tattctgaca gtccgtaaac ttccaaagca ttttttacaa agaaagagtc
    10561 gatcagctgg aaaaagataa gcaatccact ataaatcgaa accagtccgc cttctaccag
    10621 aaaacgccgg atcaagggct ttgacggctg tttgaacaat ggaaaccgtc ggaagcttaa
    10681 cgctcctcca tgaatctttt gttcatagta gtgaaggatc ccatatgcgc ataaaccgcc
    10741 aaaaacagct ccactcattg ctgccgtccc tgtttggtac accgtccatc caagctttct
    10801 aaaagcaaac gcactacaga caatcacaaa aacacgaacg aactgctcaa ctacttgaga
    10861 aacagcagtt ggttccatca agaatcttcc ttggaaattc cctcgataaa aagatagacc
    10921 aggcatcagc aaaaaggtaa aggaaactac ttggatcagc ggtttcaatt gtatgtctcc
    10981 catcatccaa gcaatccagc tgctaaatgc aaagacaaac aaccacaaaa agacacctgt
    11041 ccaaaatact aagggataaa gattttgcaa agcttgcttt cgctcgaaag gattctttct
    11101 ttctgcaacg tactttgata tgaactgcgg aagaccggat aaagctaaag tcattgctat
    11161 cccataaatt ggataaactt gctgataaac ataaaaacct tcgtcgccga ctaagttttg
    11221 gaggggaatt cggtaaaggg cacttaatac ttttgctatg aaagaagcaa tcgtcaatac
    11281 aaatgctcct tgcatgacgc gccgcatttc tttatgatcc aaatcattgc tccttcctac
    11341 gctgacagat atttttgctc acgcaacgct ttgacaaact tttggatttc ctgaagccat
    11401 actgcttctt ccatttggtt ggggatcgtc aagcgtatca ccatctgttc tttttcaacg
    11461 cctagactgg cttttagttt tgtcgctgtc agtgcttcga ataactgttc cacactatag
    11521 cgttttgttc caactttact tagagtaaag actatatgcg cctcctgttt ccttatcgtt
    11581 tctaggagcg ctcgatcgcc atccattttg atctgcccaa ttgtcaagag atgtgctact
    11641 tcttctggat attctccaaa acggtcgagc agatcatctt ctagttcatc tagcatttct
    11701 tgcgaatcaa gttcacgaat tcgtttatag atctcgattt tctgccgttg atctgccacg
    11761 tatgtttcag gtagatacgc atcgacaccg aggtcgatct caacagtggt cttctccact
    11821 tgactattct ttccttgttt acgagaaaca gcttcgctta gcatttgaga atacatatcg
    11881 aaaccgactg catcaataaa tccatgctgc tgagcaccta agagattacc tgcaccgcga
    11941 atggaaagat cccgcatcgc aatcttgaat ccagatccca gttctgtaaa atctttgatt
    12001 gcctgaagac gtttttcact gacttcgtta aggatcttct gctgctcata cataaagtaa
    12061 gcatatgcga cacggctgct tcttcctacc cgccctcgca attggtataa cgtggataat
    12121 cccatataat cagcgttctc aacgaacaac gtatttgcgt tcgggatatc tactcctgtt
    12181 tcaatgatgg ttgtagtgac aagaacatca tattgacctt caataaattc aaaaagcgta
    12241 ttttctaatt gcgcctctgt catttgccca tgagcataac cgatccgtgc ttccggaacg
    12301 agttcttgga tttcttctac tttttgttcg atcgtttcta cccgattata taaatagaat
    12361 acttgtccac ctcgtcccat ttctcgatgg attgcttcgc gaatagcccc aggatttttt
    12421 tccataacat acgtttggat cggataacga ttcgcaggcg gtgtttcgat tactgaaaga
    12481 tcccgcacgc ctaacatcga catatgcaaa gttctcggaa tcggtgttgc agtcagagtc
    12541 aaaacatcca cttgtgcacg taactgtttc aatcgttcct tatgttttac accaaaacgt
    12601 tgttcttcgt caatgatcag tagacccaaa tcgctaaatt caatatcttt tgacaaaatt
    12661 cggtgggttc ctacaacaat atcaacttga cctgtacgca gttgttcgat ggtttctttt
    12721 tgttgctttc ttgttctgaa acgacttaat agcccgatat ttacggggaa accttcaaat
    12781 ctttccagca tcgtttcata gtgctgctgt gccaagatcg ttgttggaac taaaaaggct
    12841 acttgtttgc tttcctttat cgctttaaag gccgctcgaa gcgctacttc tgttttccca
    12901 taaccaacat cacctacgag tagtcgatcc attggttttt ctttttccat atcccgtttg
    12961 atctccgctg cacttctcaa ctggtcatca gtttcagagt aggggaaggc attttcgaat
    13021 tctttttggt aggcgtcatc aggtccaaaa gcatatcctt tttctgactc tcttgcggca
    13081 tataatttga ttagatcgtc agcgatatcc tcgatttttg aagagacttt tcgcttggtt
    13141 ttcgtccatt cacttccgcc tagcttattg atgcgaggcg ctttggcttc agaggcgaca
    13201 tatttttgga tcagattcag ttgagtaaca ggaataaaca gtttgtcgtc gttttgatac
    13261 aaaatagtca tatagtcttg gtgaacgcca tctacttcta gcgtttccat cccaatgtat
    13321 ttcccgatac catgattggc atggacaacg taatctcctg cttttaactc attatagctt
    13381 ttcaatcgct ctgcgtttgt tactgtttga cgtctcgttt gttttttcgt cgttttttgg
    13441 aaaatttctt tttctgtgat agctactatt ttttcttgag gaagttcaaa accagcctgc
    13501 aaagctcctt caactaattg tacctgtcct ctgatcaatg catttttttc tgtttctact
    13561 actaatatgt cttcatctcg aagcatttgt tctgctttgc gtattctgtc ctttgtcgga
    13621 ataaagatca caacagtctg tttttgcttg cgccaacggt ccatttctgt tttcagcaat
    13681 ggcatttgtc cgaagaactg ttgcatcgtc cgatattgaa aattatggat tgcttggaaa
    13741 cgaagatttc ccatcccttt ttggaagagt gagaaaaagg ttgtggtgaa cttttccttc
    13801 tgcacgattt tttgtatatc tgctgaaaat gtctgctcag aaaacacccg caattcgctg
    13861 attttttgag tctgccattc tgccgcttcc ctttcgattt cccgatttgt ttccatgatt
    13921 cgctgataat catcaacgac tagtaaacta tttgaaggga aataatccaa gatcgtcaca
    13981 gattcttggt aaagaagatc tgagtagaat tttgcttctt ctgtcgggac acctgcttgc
    14041 cagcttgaag tcagctgacc gaagtaatct tgcaaaaact cgcggtctgc ttcttctgtg
    14101 gtcgcagcaa gccgtttttc tagcattttt tgcatttgct ctattccatg ttctagatcc
    14161 tctggtccgt agacttgttc actcgttggg gaaacccaga tttcttccgt tttttcgatt
    14221 gagcgttgtg tttctgcttc aaaataccgc atagaatcta tctctacatc aaatagttct
    14281 acacgaacag gataggttgt cgttaacggg taaatatcga taatgcttcc tcgaatactg
    14341 aattctcccg gttttgcgac catctcttct cgtacatagc ccatcaaagt caattggcga
    14401 ggaagatttt ccacgtctat ctcatcacca atatgccaat tcaattgcgc ctgtagccaa
    14461 gttttcttat tcggcagtcg tttgtgcaaa gatgcgactg gcaacaaata gatacctgct
    14521 tttttttctt gaagtgcgtt aagcgttgca actctctcgc ctcgcgcttc tggagaagaa
    14581 aaggccattt cagcagaaag cacttcatcc actggaaata aatggatctg atcttgcgga
    14641 atcacatgtt gcagatcttc tgctaactga ttcgcataat acaaatttgg catcagaata
    14701 atcatgtttt tatttgtttt cttaaacgct cgagcaaata gaagtgtttt cgctgagccc
    14761 gctaatcctg tgattagttg tctaggcatt ttttcttcta aattgctgac ccattctttt
    14821 acttgcggat cttttccgac taattgaata atatccattc cttttcctcc ttcctcaatt
    14881 gaatcgattc atcgcatcca cgaacgaatg cccttctatg agatattcga ctgctttaac
    14941 agattgacac atgctttctt ctatcaaagg ctgattttct ttgctgaaag gactaagcac
    15001 atgctggact actgtttttt tcccttctgg cctgccgatt cccaccttga ttcgatcaaa
    15061 gacattcgta ttcaaatggg aaataatact tttgataccg ttatgcccgc cggcacttcc
    15121 cttttgacgg agccggattt ttcccactgc cagatccaaa tcatcgtaga ttacgactaa
    15181 ttcctctgga taaatcccaa aataagtcat tagtgggccg actgcacgac cagattcatt
    15241 catgaatgtt tgtggtttga ctaaaaggat cttttctcca ttatgaaaaa attcggcaac
    15301 ttctgcttcg aacggatttt ttttgaaagt agcttgatgc tcgcgtgcaa ttttatccac
    15361 gaccataaag ccgatattgt gttttgtata ttggtatttg gtacctgggt ttcctaaccc
    15421 gacgatcatt ttcatttttg ttttccacct aattctaaaa agcagcacaa aaggctggac
    15481 tgctaattag cggtccagtc catcgagctt ttttcttttt tatacttgtc aatgcatagt
    15541 ataacacaaa acaaggcgtt tcggctaaaa gagaatcgtt aataaatgat aaataaatta
    15601 aaaatttctt caaagtttaa tgaaataatt gatatataaa aatagtgaaa taacgcacat
    15661 gtaagctttt acatataata caaccttgtt atctgttata ttttatagcc aaatagcgtg
    15721 acatggggct tgagaaatgt tagcataagc atgggaaaaa acaaatcaat ggaaggattg
    15781 atactaaatg actgcagctg cagaaaaaaa agatcatcaa aaagttattc ttgttggaga
    15841 cggcgccgta ggttctagct atgcctttgc tctagttact caaaatatcg ctcaagaagt
    15901 aggtattgtg gatatcaata ctgctaaaac tgaaggagac gcaattgact tgtctcacgc
    15961 tttggcattt acttcaccta aaaaaatcta ttctgctact tacgcagacg cacatgatgc
    16021 tgacttagtt gttattacag ctggcgcacc acaaaaacca ggtgaaactc gtttagactt
    16081 agtaaacaaa aacctaaaaa tcaaccgcga tgttgtcaca caaatcgttg aatcaggatt
    16141 taacggtatc tttttagttg ccgctaaccc agtagatatt ttgacttact caacttggaa
    16201 attctcaggt ttccctaaag aacgcgtaat cggttcaggt acctctcttg actcagctcg
    16261 tttccgtcaa gcacttgcgg aattagttga cgttgatgca cgtaacgtcc atgcttacat
    16321 cttaggtgaa cacggagatt cagaattccc agtttggtca catgcaaatg ttgccggctt
    16381 gcaaatctat gaatgggtaa aaaacaaccc agaaatcgat gaagaagcta tggttaacct
    16441 attctttggc gtacgtgacg ctgcttacac aattatcgag aaaaaaggcg ctactttcta
    16501 tggtatcgca gtagcattag cacgtatcac tcgtgccatc ttagatgatg aaaatgctgt
    16561 tcttccttta tcagtattca tgaatggcga atatggattg aacgacatct acattggtgc
    16621 acctgctgtt atcaaccgtc aaggtatcca aaaagtaatc gaaattcctt taagtgacag
    16681 cgagcaagac cgtatggctg cttctgctaa gcaattaaaa gaaattctag atgaagcttt
    16741 tgctaaacta gacgctgaat aatctttgaa catgacggaa aacatctatt cggtgttttc
    16801 cgttttttta ttttatcacc cttatggttt ctaaattttt tcataaagta gagataacct
    16861 tcgtcttttc ctctaaatta tgctatcatg agaccgttcc accctaaact tttacttaag
    16921 gagttgtcga tatgacaaga tcttcgcatc gaaaaaaatc gaaacatgtt gctttgcttt
    16981 cagttcttgg tgttctctta gtgctgatca gcttttatac ctatcgcagt acatactaca
    17041 caaagcattt tttgccgaat acagaaataa atggaataaa tgtcagcaat ctaacagtcg
    17101 aaaaagcaaa tgaaaaatta aaagaagctt attctgataa aaaactggct attaaagaag
    17161 atggaaaagt ctggcaggaa gtaccaaaat ccgagctcgg ctacaaagat gatttcactt
    17221 cggagctttc tccgatccta aatgaacaaa acggatggac ttggggaatg acttatgtct
    17281 cagcggcaga aaaacaagaa atagatcctg tcagtcaaaa ccaccaaaaa ctagacacta
    17341 ccgttcagac attgacaact aagctgactg atttgaacaa agaccggaca cctactactg
    17401 atgcaacgat cgaaaaagca ggtgactctt tcgttatcaa gcctgaagtc aatggaaata
    17461 cgatcgacgt cgatgctgcg gtcaaacaat taaaaagtgc ggtaaactct ggtaaagata
    17521 caattgaatt gactgaattc aaagaaaaac caaaagtaac atctgaagac agctcactga
    17581 aagaacaact cgcatccatg aatgctatcg caaatgtaaa agctacttac agtataaacg
    17641 gtgagacttt ccagatccca tcaagtgata ttatgagttg gctgacctac aatgatggaa
    17701 aagtcgattt agatacagaa caagtacgtc aatatgtaac agatttaggc acaaagtata
    17761 acacaagtac gaatgacaca aaatttaaaa gtacaaaaag aggagaagtt acggtcccag
    17821 ttggcactta cagttggacg atccaaacag acagtgagac agaagcattg aaaaaagcga
    17881 ttctggcagg ccaagatttt actcgttcac ccattgtgca aggtggcaca accgctgatc
    17941 atccattgat cgaagatacg tatatcgaag ttgatctgga gaaccagcat atgtggtatt
    18001 acaaagacgg aaaagtggcc cttgaaacag atatcgtttc cggaaagcca actacaccta
    18061 cacctgctgg agtcttctat gtttggaata aagaagaaga tgcaacttta aaaggaacaa
    18121 atgacgatgg aacaccttac gagagtccgg taaactactg gatgccgatt gactggacag
    18181 gtgttggtat ccacgattca gactggcaac ctgaatacgg tggagactta tggaaaaccc
    18241 gcggttcaca cggctgcatc aacaccccac caagtgtaat gaaggaatta ttcgggatgg
    18301 tcgaaaaagg aacgcctgta ttggtcttct aacaaaaata atatataaaa agaaagcacc
    18361 cgtacgattg gtacaggtgc tttcttttta ttatctaaca atgtgctact tacatttcag
    18421 cagcagtaaa gacttctgaa acatcatcat cgtcttctag cttatctact agacgttcca
    18481 attgctcttt ttgttcctca tttaaatcaa tcgtcgtttg tggaatcatc gtcaattctg
    18541 cttgcgccaa agtgaactct ttttccaact catctcgtac agcagcaaaa tcttctggtg
    18601 cagtataaat ctcgaatact tcaggagaag tttgcagatc ttctgccccc gcctctaata
    18661 catcctcaaa catcgtctct tcatcaacag tcaacccatc gcgttcaata gcgatatatc
    18721 ctttgcggtc aaacaagtaa ctgactgaac ccgtttctcc cagtgatcca ccgtttcgag
    18781 taaaagctac tcggacattt gtcgctgtcc gattacggtt atctgtcaat gcatggacta
    18841 agattcctac tccacccgga ccatatcctt catacgtgat ttcatcgtaa ttcgtttcat
    18901 ctacggcact cgtagccttt ttaatcgccc gttccacatt gtcgttaggc atatttgcag
    18961 cttttgcttt gtccacaaca agccgcaaag ctggattcat agatggatca ggaccgcctg
    19021 ctttagctgc catataaatt tcacgagaaa gtttctgaaa gactttccct cgttttgcat
    19081 cttgggcatt ttttcttcct tggatattat gccatttact atgtcctgac atgatttttc
    19141 ctcccttgtt cttttgagaa tacacttcta ttctagtact atttcacaaa aaaacaaagc
    19201 cacagccaat ctataaacgc ttcttgaatg ccgcaaaaag cacacataat atcacagcag
    19261 tcagagcacc gatcgaatcc aacatgacat cttggaacat aggtgttcgt ccaccggtca
    19321 tcatctgatg gaattcgtcc aaacctgcgt accctgttgc tgaaagccaa gcaaggatcg
    19381 ctgtcagcca ccaaattttt agcttgggga aaacacctaa aaacaagctg cctcccaaaa
    19441 taaaataggt accgaaatgt gcgcccttcc ttaaaaagaa ctctaaaaat ttggcgtagc
    19501 ccagatggtc gatactcacc atacttccac cataattaaa acgtataccc gctaatgtgt
    19561 ctttaaatgg ctgattaggc agccagcttt ctaatcgcga aacttgtgat tgctgtccgt
    19621 aagtttgaga agaactgtaa aataagatta taacgactat cagtgcgatt actaaataaa
    19681 tatttccgtt tttcatttgt ttttttgaag aattcataag tcacctctgt attttatctt
    19741 agactttttt tgaagaaaca acaatcattt tcatagagac ttcttccata aaaaagtgct
    19801 atacttaatt ttgtattaca atatgaaggg agttttttca tgagttattc attaaattgg
    19861 aatctagatt ctattttttc cggaggcagt cattctgatg ctttgaacca acggatgaag
    19921 caattggaag accaaatgaa cgagtattat caccgcgtaa caaaatggtc acctagttct
    19981 gacaatgcag agcagttaaa cgctattctt caattgcaag aaacgattac gaatggtttt
    20041 acccaatgca gcagctatat tactgcattg ctttctgcca atgtcaatga ttcagatgca
    20101 aaggtcttat caggcaagct ttacgcaatg ctgcctcgtc tgcaatcagc cgaaactgta
    20161 ttgtcaaaga aatttgcaga aatatctgac aatgattgga atcagatgct ttctcaatct
    20221 tcgtttgaaa caattgcttt tcgtttaaac gagattcgtc gtgatggctc acaattatta
    20281 tcagaagctg aagaaaacat tataaatacg ctttctttag atggtctaaa tgcttggagc
    20341 agtcattatg atacgattgt agccagcatt tcgattccct tcgaacaaga tggacaagta
    20401 gtcgaattat ctgctggaca agccttcaat aagatgatgg gcgatccaga tccaaaagtc
    20461 agagaaacac ttttcgcagc atgggaagaa gcctgggaag aaaaggcacc cctcttcacg
    20521 gatactttga atcatttaga tggttttaga ctttctgact atgaacttca tggtgtcaca
    20581 gattttctac aaaaaccatt agagtacaat cggttgaaga aagaaacatt gtctgtcatg
    20641 tgggatacaa tccagaaaaa caaacaaccc cttgttaatt acttgactag aaaagctaat
    20701 cttttcggta aagaaaaaat ggaatggcaa gatcaagatg cacctattat tttaggcgat
    20761 ttgaaagaaa aaacctttag ttttgacgaa gcagcagcat ttattatcga aaactttaac
    20821 aaattcagtc caaaaatggc taaatttgcc caatctgctt ttgaaaagag ctggatcgaa
    20881 gcagaagatc gtcctgggaa acgtcctggt ggttattgta ctgagttacc agaaacaaaa
    20941 gaatcaagga tctttatgac ctacagtaat tctgttaatg aggttgcgac acttgctcat
    21001 gaactaggac atgctttcca tagcagcacg atgtgggatt taccttcttt aaatcgcgaa
    21061 tatgccatga atgtcgcaga aacagcaagt acattcgcag aactgatcgt agccgatgct
    21121 acattaaaag tcgcaaaaac aaaagaagaa aaaatcaatt tgctagatac aaaactgcaa
    21181 aatgctttag ctatgttcat gaatatccat tcccgattca tttttgaaaa tcgattctat
    21241 gaagcacggc aagaaggatt agtctcagaa gataagatca cagagatgat ggttgaggcg
    21301 caaaaagaag gctatcaagg agctttagct acttaccatc catatttctg ggctgcaaaa
    21361 ttgcatttct ttattgatga cgtaccgttt tataacttcc catacacatt tggctacctc
    21421 tttagtttgg gcatctatgc ttacgccaac caaaagggag caaattttga agaagaatat
    21481 atccatttac tacgcgatac tgcctcaatg acgacggaag atcttgcgaa gaaacatttg
    21541 aatgtcgatc ttacccaacc agatttttgg caagcaggta tcgacatggt gactgaagat
    21601 atccaaacgt tcatggaatt gacagaagaa tttatctgat ttctatttta tcacaata
//
LOCUS       DACCDF010000043        21218 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCDF010000043 DACCDF010000000
VERSION     DACCDF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.044"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..655
                     /locus_tag="IVK51_001638"
     CDS             <1..655
                     /locus_tag="IVK51_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431237.1"
                     /translation="KIVLKTQSPLEVKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEV
                     IRKYDVQAKIIFTTTHDEMLPVTIKRRVETLGFVTKDQTLDEYRNEIVELLLLAQERI
                     DATKQVTNQAFVFSIGSQTFTFDINDIYFVEASKLPHRLSLCTKDGQYEFYGRISELE
                     KKYPMLTRISRACLVNIFNVKEIDFKKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1358..1573)
                     /locus_tag="IVK51_001639"
     CDS             complement(1358..1573)
                     /locus_tag="IVK51_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP6431238.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1592..1828)
                     /locus_tag="IVK51_001640"
     CDS             complement(1592..1828)
                     /locus_tag="IVK51_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP6431239.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2086..4239
                     /locus_tag="IVK51_001641"
     CDS             2086..4239
                     /locus_tag="IVK51_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP6431240.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4208..5623
                     /locus_tag="IVK51_001642"
     CDS             4208..5623
                     /locus_tag="IVK51_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP6431241.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            5656..6690
                     /gene="sppA"
                     /locus_tag="IVK51_001643"
     CDS             5656..6690
                     /gene="sppA"
                     /locus_tag="IVK51_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6431242.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            6692..7213
                     /locus_tag="IVK51_001644"
     CDS             6692..7213
                     /locus_tag="IVK51_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6431243.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7210..7596
                     /locus_tag="IVK51_001645"
     CDS             7210..7596
                     /locus_tag="IVK51_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP6431244.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(8092..8164)
                     /locus_tag="IVK51_001646"
     tRNA            complement(8092..8164)
                     /locus_tag="IVK51_001646"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8129..8131),aa:Lys,seq:ctt)
     gene            8545..9114
                     /locus_tag="IVK51_001647"
     CDS             8545..9114
                     /locus_tag="IVK51_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP6431245.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            9101..9961
                     /locus_tag="IVK51_001648"
     CDS             9101..9961
                     /locus_tag="IVK51_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6431246.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            10151..10855
                     /locus_tag="IVK51_001649"
     CDS             10151..10855
                     /locus_tag="IVK51_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431247.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            10860..12695
                     /gene="walK"
                     /locus_tag="IVK51_001650"
     CDS             10860..12695
                     /gene="walK"
                     /locus_tag="IVK51_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP6431248.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            12692..14008
                     /locus_tag="IVK51_001651"
     CDS             12692..14008
                     /locus_tag="IVK51_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431249.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            14009..14878
                     /locus_tag="IVK51_001652"
     CDS             14009..14878
                     /locus_tag="IVK51_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431250.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESINAFSNT
                     IVTNNTVPKVEDQ"
     gene            14933..15742
                     /locus_tag="IVK51_001653"
     CDS             14933..15742
                     /locus_tag="IVK51_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP6431251.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(15845..16474)
                     /locus_tag="IVK51_001654"
     CDS             complement(15845..16474)
                     /locus_tag="IVK51_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP6431252.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            16644..17936
                     /locus_tag="IVK51_001655"
     CDS             16644..17936
                     /locus_tag="IVK51_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6431253.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(18012..18674)
                     /locus_tag="IVK51_001656"
     CDS             complement(18012..18674)
                     /locus_tag="IVK51_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP6431254.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            18899..19183
                     /locus_tag="IVK51_001657"
     CDS             18899..19183
                     /locus_tag="IVK51_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP6431255.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            19234..20859
                     /gene="groL"
                     /locus_tag="IVK51_001658"
     CDS             19234..20859
                     /gene="groL"
                     /locus_tag="IVK51_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP6431256.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7235 a         3434 c         4269 g         6280 t
ORIGIN      
        1 taagatagta ttgaaaacac aaagtccgtt agaagttaaa aaatacctca aacaattccg
       61 accaaagcaa ggaatatatt tcttagatat tgatttaaat catgaagtta acggtataga
      121 attagcagaa gtaatcagaa aatatgatgt tcaagcaaaa atcattttta caactactca
      181 tgatgagatg ttacccgtaa caataaaaag aagagttgaa acgttaggat ttgtaacaaa
      241 agatcaaaca ctagatgagt atcgaaacga gattgttgag ttattgttat tagcgcaaga
      301 aaggatagat gcaacaaaac aggttacgaa tcaagcattt gttttttcca taggttctca
      361 aacatttact tttgatataa atgacattta ttttgtagaa gcttcaaaat taccacatag
      421 attatcattg tgtactaaag atgggcagta cgaattttat ggaagaatta gtgagcttga
      481 aaagaaatat cctatgttaa caagaattag tcgagcgtgt ttagtaaata tctttaacgt
      541 taaagaaata gattttaaaa aacgttcact atattttgat tcagagttgg ccagaaactt
      601 tactttaggg aaagcacaaa aaattaagga gaagttgaaa gaaagcactg tttagtggtt
      661 ttttctttag ggagattgca tttttctaat aaaatagatt ccttcactag tgcaaatttt
      721 tctgaatcct attaaagttg taccaatttt tatagctgtt attagttacg tgcataacag
      781 caatattagt tactggagga atatttctat ggtctggaac caaaaaaggg ccaaatgaat
      841 agttcttttc ttgtttttat taagtaccat aaaggggaat taaaattaaa tgtatggtgg
      901 aagatgcgaa agatttggca aattctacta atttttagaa agtgtttttc attttttagt
      961 taaaaaattt gtcctgtcaa gtgcaatcct atgattgtac ttgataggat ttgagataat
     1021 tactttcctg tttttataaa tttaacttgc aggaacaggc tttaatgcac atccgacaag
     1081 tacattacca atacctcctg taacggccga aataggtcct cctgcagcac caactacagc
     1141 gctgcaagtc cttttgtaaa aatatctcta ccattacatt tccaaagaat ttttatctga
     1201 ctttaataaa agataaaaat ttaatataat tataaaaact agcacaagaa gctttagtaa
     1261 aaagttctgt ccattccaaa cttttgaaaa gataaagaac atacctatgt tcgtaatcga
     1321 cacaaaccaa tcttgaggtt tgatattttt taattgttta cgcaccggtc cacatgctga
     1381 atccagcacc taataaagct ccacctacac cacaagcagc tattgcataa ggtcctccgg
     1441 ctgaacaaag ttttactcct gctgttgccc cagaaaaagt agtgcctaaa actcccacgg
     1501 cactcccacc ttgaatattt ttcaactctt ctgaagataa tataaatgtt gtttttgtgt
     1561 ttttaatatg catactactt attctccttt tttatttttt tcgagcatcc cagccagctg
     1621 cagcacctcc agcgacagct ccatataacc ctccaacaac accgcaagct ggtccgcaaa
     1681 ttgctgttcc taaacctgca ccacctactg cccctgtggt aagtccacct acaacttgcc
     1741 ccaattttcc ccaagcatta ttgcctccac caacagtttg tgcatttcca caatacttag
     1801 ttcctttaca ttttgcatgt ttaaacacgc ttctttaaat aatatttttt acggtgctac
     1861 tgtaacatac gtatttgatt aacagtaaaa atcttcctga aacttatcaa gtttattctt
     1921 gaatagtaaa atttccaaaa taaatttgat tcaggaatga aaaagttagt gttcaagaac
     1981 tgttttcaaa gtttttgtgg aattatgaga caatattttc actgtattaa agctatattg
     2041 agaatttttc tataaaaatt ttttagaagg agttgtgtta gataagtgat aaaatcattt
     2101 ccttttgtgc agcaacaaga tgaaaaagat tgtggtgttg cttgcctttc gatgatttta
     2161 aaatatttta aaactgatat cccgattcat aaattacggg acctatctgg gactgattta
     2221 gagggtactt cagcatttgg attgaaaaag acatttgaaa aattgtcttt tgattgtatg
     2281 gctattcaag cagatagtga gatctggaaa gaaagagatc ttatcttacc attaattgca
     2341 catatattgg ttgataacag ctatatgcac tacgttgtaa tttataaaac gaagggaaac
     2401 gtactatgga ttgcagatcc cgctaatgga aaaaatagaa aaacgattga agcgtttcaa
     2461 aaagaatgga caggtattct tttattacct atgcctagag aaacttatat tcctattaaa
     2521 gaaaaagtag ctggattaag ttcttttttg cctataattt ggaaacaaaa aggattagta
     2581 ttccacatcg ttttagcatc gattttcatt acgttttttg gcataggaag ctcatattat
     2641 tttcaaggta ttcttgattt tttcatccct aatcaagcac gttcaacttt aaatataatt
     2701 tccattggct tgatcgctgt ctatttattt cgggtattgt ttgaatatag tcgtagttat
     2761 cttcttatta ttttaggaca gcgaatgagt atggcaatta tgcttcagta tttcaaacat
     2821 gtgttaactt taccaatgaa tttttttgct acgagaaaat caggggaaat tatttctcgt
     2881 tttcttgatg ccagtaagat tattgatgct ttagcaagtg ctacgttatc aatattttta
     2941 gatattggta tggtgttgct tgttggggtt acgttagcta ttcagaatag tacgttgttt
     3001 ttaatcactc tatgctcttt gcctttttat gtagttgcaa tattcgcttt cgtgaaaagc
     3061 tatgaaaaat cgaacaggga agaaatggca gccggtgcag ctgtaaattc tagtatcata
     3121 gaaagtctta aaggaataga aacaattaaa gcatatagcg gtgaagagaa agtatataac
     3181 cgtatagacc atgaatttgt taaattaatg aaaaaatcat ttcgtacggt tacgcttgat
     3241 aatattcaac aaggaattaa acatttgatt caattaatta gcagtgcatt aattttatgg
     3301 ctaggttctt attttgttat ggataataga attagtctag gtcaacttat cacatacaat
     3361 gctttactag tatttttgac agatccttta caaaacatta ttaatttaca agtgaaaatg
     3421 caaacggctc aagttgcaaa taatcgttta aatgaaattt tcgcgattga ttcagaaaaa
     3481 tcgaaccagt cttttgaata tatgctttct gaacaattat ttcaacaaga tatcactgta
     3541 aaaaacgttt cattctcgta tagcatgaag gccccaacgt tgaacgaaat ttcatgtaca
     3601 attccttcgc acagtaaaac ggctatagtt ggagttagtg gttctggaaa atcaactctt
     3661 gcaaagttat ttgttcgttt ttatgaagca tcagaaggat cgatccatta tggaaacatt
     3721 aatcacttag acattccttt tcagatatta cgcgatcgag tgacatattt gcctcaagag
     3781 tcatttttct ttagtggaac aatcattgag aatcttatat tcggtttaga tagcccacca
     3841 agtttcgaac aaataataga agtttgtgag atagtccaac taaatgaatt tattaatcaa
     3901 cagccattac gttacgagac attgttggaa gaaggtggaa ataatctttc aggagggcaa
     3961 cgacaacgat tagccttagc acgggcttta ttaaaaaatc cggacatatt aattttagat
     4021 gaagcaacta gtggattaga taccttgtta gaacatgcaa ttatgaatta tctcctcaat
     4081 ttaaaagaaa aaacgattat ttttattgcg catcatttac caatagcaaa agcatgtgat
     4141 caaattttag ttctccatga agggcatttg gtagaaaaag gaacgcacga tgaacttcgt
     4201 tttgccgatg gtatctatca aaaattatgg gaaatctagg agagggaaac aatttatgca
     4261 gaataataat tggttcgaac attctagtat ctatactaaa caacatcata gcttctaccg
     4321 ttgggtgtta taccctgtaa ttcttttttt atgtattatt ggattgtttt taacctttgc
     4381 taaaaaagag gttgttattc ggacaacggc tcaattgaac cccgaaaaaa tagagaagtt
     4441 acaagtgcca ttagaagcta aaataatgga aaacttccta aaagaaaatc aatttgttca
     4501 taaggacgat gtgttggtta gattggattg ttctttaata gagaatgaaa aggctcagat
     4561 agaacaagaa aaccaacgta ttactcaaca aataaagatg gctcagctat ttattgaaag
     4621 tataagtaaa ggaaaaaatt tgttttcaac ggatgacagt tttggctaca gtaatcaatt
     4681 aaagagcatg ttgtcagaaa aagaatcact ccgctacgct ttgaagcaaa gtgaattaaa
     4741 tgatcaaaag caattagaag tatacgaaaa gacaaaaaga caactagaaa aacaaattga
     4801 gagttcagat agtaaattac aagaatggca acaagtacag gtagcttgga gtaataatca
     4861 atcattaaaa gatttttcaa aagaaatgat ggcaaactat gagaattggc aagaacaact
     4921 aaataatgtt tctgatgatc aaaaaaatca agtgaaactg acaatttcag caagcataaa
     4981 tgaacaaatt gagcaactaa aaaaagaagt agaacagtat cagtcagaaa aagctaaatt
     5041 agttaaacca actacttctg agaatgacag aattagtcaa acggaaaaag gaaagcaaga
     5101 gctagaacaa gcaataacaa ctgtaaaaca aacagttaca gagctaaatg aaaaacaaga
     5161 gaagaaccag tcagttatca aaagtttgga cgatcaactt tcaaaaggaa tactaaaagc
     5221 tcctgtatct ggaacggttc atttaaatga agaaacaaag ggacaagcag aactagccaa
     5281 aggaacagtg cttgcagaaa tttatccgat ccacgaaaaa tcaaaaatga aatttacagc
     5341 acttttacct gcgaatgaaa gtacctatat taaagaaggg atgaaggttc attttaaatt
     5401 ggatcaaaaa ggtggggctc cgattacgat cgacggaact ttagatgaaa tttcagaaaa
     5461 ttctactgcc actgagaaag gtgtttttta tgcagtgaaa ggaacgttaa aaacaactca
     5521 atcgtttccg tttcgttatg gattaactgg agagttgtct ttaattgtag gagaaaaaac
     5581 atattggcaa caaattgtcg atgtgtttct aaataaaaaa taattatttt tactaagaat
     5641 ataggaggag tttatatgaa taaaagaaga tggattgctg tgggagtagc gtgcggatta
     5701 ttgttactat ccattatcgt cgcgctaatt ccaggaaaag ataaagagga agcatcaaat
     5761 acaaccctaa caggaataaa taagattttt tacggatcta acgaaatcac agaagaaacg
     5821 ttagaagaag gcgcatcaaa taaaaagatt gtcaaactat ccgtcaatgg agttattgca
     5881 gatacaggtg aaagcaatct ttttagtagg gaacaatata accatcagaa ttttttaaca
     5941 cagctaaaga aaattcaaga agacaaagcg gttaagggag tgttattaga agtaaattca
     6001 cctgggggag gaatatatga aagtgctgag attgctaaag aaatggcaaa aattaaaaag
     6061 ctagatattc ctatctatac tgcatttaaa aatacagcgg caagtggagg gtactacatt
     6121 tctgctggtt cagataaaat atttgctacg gaagaaacaa caactggatc aattggtgtc
     6181 attatttctg gtttaaatta ttctggctta ttagaaaaac ttggcgtaac tgatgcgacg
     6241 tataaaagtg gtgcattaaa agatatgatg tcacctcaac acaaaccttc agaggaagag
     6301 aataaagtta tacaggaatt tgtgatgagt gcttatgatc gttttgtcaa tgttgtagca
     6361 aaaggaagaa atatggatac taatgcagtg aaagaacttg cggatggaag aatttatgat
     6421 ggtaaccaag cagtagaaaa tgggttggtg gatcaaattg gttactctga agatgcatta
     6481 gattccctaa aaaaagagaa gaaattaact gatgcgacgg ttatagaata taaaaatgac
     6541 acaactggct ttgcttcttc ttggttaggt aataagattg ctgaatggca agggctaaag
     6601 gcaactacat cagatcgtgt aatttcagtt tttgaaaaac ttggattttc tgatactcca
     6661 aaaccaatgt attactatgg agggctataa aatgaataag aagagtggca ttgaaacttt
     6721 gcctaattat gtatttgcgg gattttggtt acgatttttt gcttttctta ttgatttaat
     6781 ttgtattagc atcatcaccc atgctactat aggtctcttt tacaaaatca tgggactgga
     6841 atctgacaaa tccattatta ctttttatgg tatagcgtca ttagctattt atttaaccta
     6901 ttttgtactt ttgactaagc tgaattcagg acaaacaatt gggaaaatga tttttgggat
     6961 aagagtagtg agctttgagg aaaaagagct aagttggcaa actatcttta ttcgtgagtg
     7021 tatatgtagg tttatattaa aaaattttgt atttcaaatt gggtatttgg tggctgcttt
     7081 cacgcccaag aaacaacata ttggggatta tttttctaac acgtcagtgg ttgttattcg
     7141 ttcattagaa gctattaact atggtcaaaa aatttctaat tctaaaacac ttaatctgga
     7201 gagaattaaa tgaataagag aaaaacagag tttattttga ttttaattag tggaataaca
     7261 gggattttat gtagtctttt agcattgatg ggtggtatgt ttctatcttc tattgattca
     7321 actactttaa atactacaga actaaccagt aaggatatag atatgttagt caatctaagc
     7381 gggaatgttg ttttatttgg aggaattacc ctagtttttt cgataatgat tttcataata
     7441 ggttttttag taagaaaaaa tcaaaaaaca gtggtctatg gtattagttt actcatttta
     7501 tctattattc cattttttct tttaacatta ttgtgggtaa ttcctggagt tttaggtatt
     7561 attgcgggat tgatgcttct aataagaaaa tcttaattag gcaatgtgta gacaacgtga
     7621 aacgattaat caattaaaag ctaaatacgt tttgagaaat gataacttgt agtcgatgga
     7681 agacatgtag ataaaaaaac tacgtgtctt ttatttattc taattttttc gaaaaaaaga
     7741 cactctctga ttgttgtatt aatggaaaag gggaaagcag caagtaaatt tagtttttct
     7801 attaaatatt cttcactttt taacctttta tagtacttag atttaatcct atatgtttag
     7861 agagttcatt ggcagcatga ttttgcaaag cttctcgaat tattgttata ttagaaactt
     7921 tactgttcat aattatgctt cttttttaac tcaaagtcca aagggctaca gtggactttg
     7981 agttaaaaat aaaaagccct agttattttg aaaaataaaa aaagaacagc gtaaatgttt
     8041 tcgaaatgct ttcgcaccaa catttacctg ttccgtgggt ttggcaccca atgggccgtg
     8101 aggggctcga acccgcgacc cgctgattaa gagtcagctg ctctaccaac tgagctaacg
     8161 gcccgtacaa aaagtactta ataatagtag cactaagaat tttaaaatgc aagactacat
     8221 ttaaagaaaa tcagaaaaat ctttacttcc ttataggaaa tcattcatag attattcaca
     8281 tttgctcgct attatataag cataccaaca aacaaagttc ttgcaagaga acaaacccta
     8341 acactttttt cattttttac tcctttcccg ccgtctcccc ggcgggtctc ctgttcttaa
     8401 cgataaaaga caagaaggct tagccttctt gttttttgtt ttcatggaat cccgctaatc
     8461 aaatcaccta gttaaagcaa ctagattgaa tgtttatcat aagtctgata tcatgtttat
     8521 gaaatgaaaa aaggagcggt tatgatggaa aaaatgcgtc agcaattaat ggagtggggg
     8581 aaggaactag aaacattccg tctgcctcac tgggaagaac tccctgactt agaactttat
     8641 atggaccaag tcagaacgtt agttgatcgt tatctttcac ctgtgataca aggagaaaag
     8701 catccattgc ttacctcttc tatggtaaac aattacgtta aactaggttt gataccagca
     8761 ccagttaaaa aaagatataa caaagaacac gtagcatttt tacttgcaat cactacgttg
     8821 aaacaagtat tgactattcc tgaaatcaaa gaagggatat tgttccaagg aaaaacagta
     8881 ggaattcgtg aagcctataa tctattttgc gatgaacaag aagcagctgt gtggatggtc
     8941 agccaattag cacaaggtaa atctcatccg caaaaattcg accaggcaac ccctgtagaa
     9001 tatattgctg taagagcggc aactttatcg tttgcaatga aacttcttgc ggaaaaaacg
     9061 attgttctag aaaccgaata tttaaaagag gagaaaacaa atgaaaaaca ataaaatcgc
     9121 aattttagta gattcaggta cggatgttcc agaagaattg ataaaaaaat acgagatgta
     9181 tatgatccca ttgaaaatta tttataaaga tcaggtatat acagataaaa ttgacattac
     9241 ccctgaagag atctatcaac gtttgccaga ggaaatccct ggtacatctt tgccggatgg
     9301 tgagtcgatc acagaaatct ttgagcaaat caaaaaagac ggttatgaaa aggtacttgc
     9361 ggtaacaatt tccagtggat tgagcgggac atataatgtg gttcgtttaa ttgcacaaca
     9421 gcaagaagaa ttggaagcat atgtactaga tacgaaaaat attggtattg gtgcaggttt
     9481 ttctgcgatc caagcagcaa aatggctgga agaaggagta gaatgggatc aactgatcag
     9541 taatctgaat gagttagtcg aaagaacaaa agttttcttt aatgttgcga cattggaata
     9601 tcttcaaaaa ggtggacgga tcggtttagt tgcttctatt gtaggaaccg ctttaaagct
     9661 taacccaatc atctcatgta atgaagaagg gatttattac acagtaggaa aagctagggg
     9721 acgtaaaaaa agtttggata aaacggttgc ttacgtcaaa gaaaaaatcg gtaatgcaaa
     9781 ggtcttcaac ttagctgttg cccatgggga tgcgaaagaa gaagcaattg aaatggaagc
     9841 gagacttaaa agagagttcc cacaagcaaa ccaaatctac tttggccaga tttctccggc
     9901 attagtagtc cacactggtc cgggattatt gggtgtcggt gtacaagttt tagaaggata
     9961 aatcaaaaaa aggaaacact gaggtgaaaa tcagtgtttt ccttttttat tgaagttaga
    10021 acaaaaaata ataaagtatt ttgcactttc attttaattt tctcttttgt tttataggca
    10081 aagatgaaac aaaagcagaa atacggtata atgagaaggt atgacaataa tatataagag
    10141 gtggctcttt atgaaaaaag ttttagttgt cgatgatgag aagccgattt cggatatcgt
    10201 caaattcaat ttagccaaag aaggctatga cgtttatact gcatatgatg gggaagaagc
    10261 cttggaaaaa gttgctgagg tagaacctga tttgatttta cttgatttga tgctacctaa
    10321 aatggacggc ttagaagttg ctcgtgaggt acgcaaaact tatgatatgc cgatcattat
    10381 ggtaacagcc aaagattctg aaattgataa agtcctaggc ttagagttag gcgccgatga
    10441 ttacgtgacc aagccatttt ccaaccgaga attagttgca cgtgtcaaag caaatcttcg
    10501 tcgcggtgcc acggctgcga aggaaccaga agaggcagca cctgcggaat tgacgattgg
    10561 tgatttgacg atccatccag aagcttacat ggtgacgaaa cgaggagaaa caatcgagtt
    10621 aacacatcgt gaattcgaac tactctttta cttggcgaag caccttggac aagtgatgac
    10681 aagagaacat ttattacaaa cggtttgggg ttatgattat tttggagatg tacgaacagt
    10741 ggatgtaact gtacgtcgtt tgcgtgaaaa gatcgaagat aatccaagcc atccaaatta
    10801 tttagtgaca cgtcgtggtg tcggttacta tttgcgtaac ccagaacagg agtaattgga
    10861 tgaaaagaaa agtccgcttt tttcgatcag tcaattttaa gatcgctatt acctttattc
    10921 tgattttatt gatctcgatc gaaattattg gtgcctactt tatacgtggt ttagaacgtt
    10981 ccacgatcaa tacatttatt aaagatatga atcaaacggt ggaatcatta gcaacaacaa
    11041 tcagcccgga attgaatcga aaagataatg cagatgatga agaggtaaat gcgaatatca
    11101 aaagatttat tgaaaacagt gcgactagcg atatcatcga aatacgggtc gttgacgaaa
    11161 aagggatcat ccgaggaaca accgatgtca atgaacaaag cgctgtggga aagaaaaatg
    11221 attatgttga tatcaatgat ttcacgacta aacgttatgt tgctttagat aacgacgata
    11281 aacgggtgaa tatcaatgtt caaccgatcc tgtccccgac cggcgatgcc gttatcgggg
    11341 ctctttacgt caaaagtaat atcgaacaaa aatattcgga aatcaataac acagcagtca
    11401 ttttctttac tgcctcacta attgcggcat ttatttcaat gatcgtatct gttttagttg
    11461 ctcgttcgat tacacagccg atcggtgaaa tgagagaaca agccattcga atcgctagag
    11521 gagactacag tcggaaagtc aaagtgcatg gacaagatga acttggacag ctggctgata
    11581 ccttcaatca gttagctgaa cgtattgaag aagctcaaga tacaatggaa gctgaacgca
    11641 atcgtttgga tagcgtgcta tcccatatga cagacggcgt cattgcgaca gatcgcaggg
    11701 gtaaagtcat tacgatcaat gatatggctg tttccttgct agatatcaaa aacgaggaag
    11761 caatcggaca atcgattttg acacttttag atatcgaaga agaatatacg ttgcggaaat
    11821 tattagaaga tccaaacgaa atggtacttg accgttcaac cagtacattg gaaagtgatc
    11881 agatgatctt gcgagtagac ttttctatga tccgtcgcga atcaggtttt atcagcggat
    11941 tagtagctgt cttgcatgat gtgacagaac aagaaaaaac agaacgggaa cgtcgtgaat
    12001 tcgtctctaa tgtttcccat gaacttcgta caccgttgac aagtatgcgt agttatatcg
    12061 aagcattgag cgaaggtgcg tggaaagacg aggaagtagc accaaatttt ttgaaggtaa
    12121 cgctagatga aacagaccga atgattcgaa tgatcaatga cctcttaaat ctatctcgca
    12181 tggatacagg taatacccag cttcaattag aatatgtcaa tttcaacgag ttagtgaatt
    12241 ttgttcttga ccggtttgat atgatggtgg gaaatcagga aaaaaattat aagatccgtc
    12301 gtgaatttac gcaaagagat ctttgggtcg aagtagatac agataaaatc atccaagtag
    12361 tcgataatat catgaataac gcgatcaagt attcaccaga tgggggaacg attacttgtc
    12421 gcttattaga aacgcataat aacgtgattt taagtatcac cgatcaagga ttggggattc
    12481 ctaaaaaaga tctgaaccga gtgttcgagc ggttctatcg agtagataaa gcacgtgcaa
    12541 gagcacaagg tgggacagga cttggattag ctatttctag agaagtgatt aaggcgcatc
    12601 gaggcgctat ttgggcagag agcaaggaag gaaaagggtc gactttttat atctcgctgc
    12661 catatgaacc ttatgaggag gaatggtggg aatgagaatc tctgaaaaag tcgttcgtat
    12721 aggactcatt ttgatggttg cgttaagcat atacttctct tatgcaatct ggctgagccc
    12781 agcaggaaaa agttcaatca acgtagatga gacaaacact caaatgatcg agtctcaaag
    12841 caatcgaaaa acttccgaag tctttcttcc gcttcatgtt acatggcacc attcgggaat
    12901 gatccaagaa accaacagtg aaaatctcgt gtaccgattg caattgatca tcgataaagc
    12961 aagatttgga aagctttctg aagtagtttc aggagacaaa tcacagtttg aacaggtgaa
    13021 aaatctgaac tctggttttg agttggccta taatgcgcct tttttgctaa gagaatacaa
    13081 ggaagcattt gatttagatt tagatgtgtc gaacttggca aaagaaagcg aaaatatgta
    13141 ttttactaga atacaattcg atcaaggaaa taaaaaaatc cgatttttga attattcgaa
    13201 ccatctcgta tatgaagcaa atatttcgtt gaattggaca gatatcaata aagaattaac
    13261 agcaaaagac acaaaatgga cagaaatgac aacagaacca tcgacgatcg acacgcaata
    13321 tgctacgaaa cattcgatca aactgaagaa gtatagttat atcctgtcac agcaacccta
    13381 tacacttttt agaaatgctt ttttccagaa accagatagc gtgaaaaata atgatgaaag
    13441 caatgaattg attttttacg gtgggaatga aacaatgtct atccattcgg aaacgcaagt
    13501 agttgatttt cgtggagagc tgccagagaa aaaagaaaat gctagtgtat tttctgaaag
    13561 tttcccctat attagcaaat taggcagttc tttagggaac ctcagatatt ttgaccgcag
    13621 tgacaatacg atcgattatc ggatattcgt ggaaggcttc ccggtttttg ggacagacag
    13681 cagaggacag attggtgtgg aagttggcgg tgaaacgaat ggttctgtgc aagtgaatat
    13741 ccaaaccagt ttgaatacga ttcaagtgcc gataccttca gacgaagaag tagaacttgc
    13801 aagtacgaat gaaatcatcc agcagttaaa agctaaggga gcagatagca acaagattgg
    13861 ttcagtgatc gttggctata catggcaaaa tgtcaaagaa acgaatcgtg tcgttgatct
    13921 cttacctgaa tggtatgtga aatataaaga taactggtat tcagctaacg accttctgga
    13981 aaaattacct ggattggagg cgaattaatt ggatttcaaa aaaatcgaat ggatattttt
    14041 cgtcgctttt ttagggttga atgtgttttt attcagcagt tatcatgacg cggtatacca
    14101 agaaaacaat gtcattcgct ctaatcagac tgaatcaatc caacaacgtc tggctagcga
    14161 cgatatcact tacacgggga agatttcgtc tgagaacaag caaggctatt atcttagcgc
    14221 ggagcagacc aatcttagca cttcattagc gaattatcgc aaggctggta aaagcggctt
    14281 gctggcaagt ggtgtgtttt tagacggtga tgttttgacg aaatctttat ccagttcaga
    14341 tgctgaaaag aacgtgatcg atccagataa aattagtagt tccttggaag attttttatc
    14401 taacaaagac aatattttat tcggtaatga ttatgtgtac acgaaaaact tttctcattt
    14461 agaagaagaa ccactagaaa tccaagcaag ccaagagtat gaaggcatcc cattcaatga
    14521 tgatacagca aaaatcgttc tttctttaga aaaaaacgat aatgattata gcatcacgaa
    14581 atacatgcag acgcatttat ctgatatcga gcagctgcgc gaaaagacgg aactgtatac
    14641 ggaagaggaa gcaatcaata cattgtacat caataacaaa ataccaagag gatcaaaaat
    14701 tttatggcgt cagctagctt attcctgtat tctgaaagtc cgggaaaaaa atgtgtatgt
    14761 tcctgtttgg catgtagcga tcgaaacaag cgataaagtg gtccaaattg agagcatcaa
    14821 cgctttcagc aatacgatcg tgacaaacaa tactgtacca aaagtggaag atcaataaaa
    14881 aagcgagtat aatgaaaaaa gtttacaaag taagaaggga agtaggagtc aaatggctga
    14941 agaaaaacca gcttttaaaa tcagtatcct agcaagtgga agttcgggta attcacttta
    15001 tatcgaaagt gaaaaaaaac ggcttttggt cgatgcagga cttagcggta aaaaaatcac
    15061 ttccttgatg gctcaaattg atcggacacc ggaccaatta gatggaatac ttgtgacgca
    15121 tgaacatcga gatcatatac atggcgtggg tgtcttggct cgtaagtatc atttggatgt
    15181 ttttgccaat cagcagactt gggaagcgat ggaaccactg attggaaatg taccagtgga
    15241 acaaaaacat ctatttgaga tggggaaagt cagaacattc ggcgatatcg atattgagag
    15301 tttcggcgtc tcccatgacg cagcggctcc gcaattttat cgtttccata aagaaaatcg
    15361 gtcattcgtc atgttgacgg acaccggata ttgcagtgat tatatgcgag gtgtcattga
    15421 aaatgccgat ggttatttga tggaaagtaa tcatgatctt gagatgttac gcatgggacc
    15481 ttatccgtgg aatttaaaac agcgtatttt aggagataga ggacatcttt ccaacgaaga
    15541 cggtgcactt gtcatgactg atgtgatcgg taatcggacc aaacgaatct atcttggcca
    15601 tcttagcaag gaaaacaata tgaaagaact tgctcattta acaatggaaa atgttttgaa
    15661 agaaaaagat tttggcgttg gacatgattt tgaaatctat gatacagatc cagatgaagc
    15721 aactgagcta ttttcgattt aaacagttca atcaataaaa atataaaaag cagaagataa
    15781 aatcaatcag ggttggtttt atcttctatt tttatttttt tagcatatta aaaaaataac
    15841 cgtattatcg actgactaca ctgatgtgct gcttttcatg tttctcatcc agtatctcat
    15901 ctactaatgc aatcgcgtaa tctgccatgc tgatcgtact gtcgctagca gcgtttcttt
    15961 gtaaataatc atcacttaac aaataagtac ctgtacgttt gccatgtgga tcaaataaag
    16021 aagctggact gatataagtc caatgtagat ttttagattg tttcaatttt aacaatcctt
    16081 cgtacatatt gaaagctgtt ggatagaagg gtgcattttt aggtacttgg tcaatcatac
    16141 gagtcgtctt ttctgtatct aagaataacg aagaggcacc cccaacgaca attaagcgtg
    16201 tatcggttcc ttcaagcatc tcaatcaaat gggacaaaga agtttgatgc atttcttctt
    16261 ttcctttggg agcgttgaaa gcatcaacga caacatcaaa agatgcaaga tcagtagttg
    16321 tcaactcaaa taaatccttt tgtataaacg gaacatctga tggcaatttt tctggatgac
    16381 gcacgattgc cgtcacgtct aaatctctgg acaatgcttc gtctaaaata aatgaacctg
    16441 catggccagt agctccaatg agtgcaattt tcataaaaaa cctccttttt tattcctgtc
    16501 cattgtaagc agttgctgaa tattcgtcaa agaatccgat ttttgaaaag ctcagtgcgc
    16561 attcctttca tggttcgtga tcattgggta tagttaaatt aagaaaactg attgataaaa
    16621 atagaagttg aaggagataa gaaatgaaag agtggttaga tcgtgccttt ttaccaatga
    16681 taacatctgt tgtgaatctt agacccttaa ctgctttgcg taatggaatg acttacacga
    16741 tcccaataat tattgttggc tcgctgtttt tcttatttgc tcattttcct gtggattaca
    16801 tcgctcagtg gatagacaaa acaggatttt ctatgtattt tacacaagta taccaggcta
    16861 catttgatgt gatagccttt tggatcgttt ttgccattgc atacgcttat gcaaaagaag
    16921 aagacatcga tcgcttacca gtcggcataa cagccttgtc tgcttatcta ctgcttattc
    16981 agccactaaa tactcagata gatactgtct ggacaggtgc tgaagggata ctaggagcag
    17041 ttacaactgg gctcatcatc ggatggggct atgcacgttt agtaagactt gcgaaagggt
    17101 ggaagcaaag agagaagata cctgaaaatg tttggcagtc ttttacttca ttgattccaa
    17161 tcattgttat agtcactttt acgcttatcg gttcagcggt ttttcaacat ttgttcagaa
    17221 tgactgttgt tcaaggagta tggacatatg tccagcttcc cattcaatcg ttcatagaca
    17281 cgttgtttgg tgtgctttta ttaggttttt ttgttccgtt tttatggtta tttggtcttc
    17341 atggatctgc gatggtcaat gggttggtta gtcccgtctt gcaagcgaat tcattagcaa
    17401 atgcagaaat cttagcctca ggacaagagt tgacggtggc aaatggcgga catatcgtta
    17461 ctcagcaatt tctcgatcag ttcatgacgg ttacgggtgc tggattgacg ttaggggctg
    17521 tattttttat gacggttttt gcgaaatcga agaaatacag agaattagga aatcttgctc
    17581 ttttaccagc ctttttcaac atcaatgaat caattatttt ttccacaccg atcgtgatga
    17641 acccgatgat ggctgttcct tttattttcg caccggtcgt ttcaggattg attacctatt
    17701 ccgctttata ttttggattc gttcctttat ttacagcagt ccaagtccct tggacaacac
    17761 cgccgatcct ctcgggtttc ttagtaggcg gagtgcctgc agcgatttta caaggtatcg
    17821 tgttgtcaat tagttttttc atttatttcc catttttgaa aaaaatagat tctgcaaact
    17881 gcaaaaagga aagacagaca aaaaatgatg gagcagcaga aaagatgaac gactaaaaaa
    17941 caaaaatact cctgtcgctt tagattggga aaatcaccaa tctaggatga caggagtatt
    18001 tatagtcatg tttatactag ccctttttgg acagctaatt gaacaagaac aactaatgtg
    18061 ttcattccta cgtgtgtgat catcgatgtc cagatccgac cagttgcttt gtactctagg
    18121 ctgaagaaga aacctaggaa aaagtagatt agcagatgac catcattatg agcaaacgca
    18181 aacagcaaag agctgatcac tgctccgacc caaaaatttg aatatcgact gatgatcccc
    18241 agtatactgc ggcggaatac gaactcttcc atgatgggtc cagcaacagt tgtcgccaaa
    18301 ataaaagcgg gtgcttccat gatcatctgg atgatatttt gtgtattctc ggaaggggtt
    18361 gctttgccaa acaatacaga ttctaacatg acagcgatgc tttgcagaat caatgcgaca
    18421 aagattccaa ccaatccata aataacaatc tttggagcag aagctttttt taccgctgat
    18481 tcgatcgcta agggttcacg ttgcttaaaa taaaaaatag ctaggacaac ggcgcctagt
    18541 atatacatga ctgcagttgc tgtgatcaat tcagacgtag attttacaag tcctatcgaa
    18601 gctaggatca agggtgagat aaaaattaga ccatagcaaa aaatgctgag gaagctatat
    18661 ttttttaaag acatgtaatt cctcctttta agggataaca agcatgcaag gcaagcttat
    18721 atcgattgat ttcaattata tcataatttc aataagcagc tgtcagaata aaatttcttt
    18781 tctttgattt aaaagcttgc aaaaaagagg gagaatgagt attattaaaa atgtaggtta
    18841 gcactcgaag gttaagagtg ctaacagatt aaaaaaagag ttatcggagg gatttatcgt
    18901 gttaaaacca ttaggtgatc gcgtcattat cgaagtcgca aaagaagaag aaaaaactgt
    18961 cggaggaatc gttttagctt ctgctgctaa agagaaaccc caaactggga cagttgttgc
    19021 agtcggtgaa ggccgtttgt tagaaaacgg tgaaaaagtt cctgccgctg taaaagcagg
    19081 cgaccaagtt atgttcgaaa aatacgccgg tacagaagta aaatacgaag gaaaagaata
    19141 tctgatcgtc gcaggaaaag atatcatggc gattgttgag taaatttgga agataaaaaa
    19201 ataatcaaaa ataatcaatg aggtgaatca atcatggcaa aagaattgaa atttgcagaa
    19261 gacgcacgcg cagctatgtt acgcggggta gataaattag cagatacagt gaaagtaaca
    19321 ttaggaccta aaggccgaaa tgtcgtacta gaaaaatctt acggctctcc attgatcaca
    19381 aatgatggtg tgacgatcgc aaaagaaatc gaattagaag atcattttga aaacatgggt
    19441 gcaaaacttg tatcagaagt agcatcaaaa acaaatgata ttgcaggaga cggtacaacg
    19501 acagcaactg ttctgacaca agcaatcgtt cgcgaaggtt taaaaaacgt aactgcaggt
    19561 gctaatcctc taggcattcg tcgcgggatc gaattggcaa caaaagcagc agtcgaagaa
    19621 ttgcataata tctcaactgt tgttgattca aaagaagcca ttgcacaagt agcagctgtt
    19681 tcttcgggtt ctgataaagt tggacatttg attgctgatg caatggaaaa agttggaaac
    19741 gacggtgtca tcacaatcga agaatcaaaa gggatcgaaa cagaactaga tgttgtagaa
    19801 ggtatgcagt ttgatcgtgg ttacttatca caatatatgg taacagataa cgacaaaatg
    19861 gaagctgttc tagaaaaccc atatatcttg atcacagaca aaaaaatctc taacatccaa
    19921 gatattctgc cattgttaga acaaatcttg caacaatcac gtccattgtt gattattgct
    19981 gacgatgtag atggtgaagc attgccaact cttgtattga acaaaatccg tggaacattc
    20041 aacgtagtag ctgtaaaagc tcctggtttc ggcgatcgtc gtaaagcaat gcttgaagat
    20101 atcgctattt tgacaggtgg tacagtaatt actgacgatc taggtttaga attgaaagat
    20161 gcaacaatcg aaaacttagg aaatgcttct aaagttgttg tagataaaga caatacaacg
    20221 atcgtagaag gttctggaga aaaagaagca atcgaagctc gtgtgcaatt gatcaaaaac
    20281 caaattgctg aaacaacttc tgattttgac cgcgaaaaat tacaagaacg cttagctaaa
    20341 ttagcaggtg gtgtagctgt tgttaaagtt ggtgcagcta ctgaaacaga attgaaagaa
    20401 ttaaaattac gcattgaaga tgcattgaat gctacacgtg ccgcagtaga agaaggaatg
    20461 gtctcaggtg gtggtacagc tctagtgaac gttatcagca aagtttctgc tgtagaagca
    20521 gaaggagacg tggcaacagg tatcaagatc gttgttcgtg ctttagaaga accaattcgt
    20581 caaatcgctg aaaacgctgg ttatgaagga tcagtgattg ttgataaatt gaagaacgtg
    20641 gaactaggca ctggtttcaa tgctgctact ggagaatggg taaacatggt tgaagcaggt
    20701 atcgtagacc caactaaagt aacacgttct gccttgcaaa atgccgcttc cgtttctgcc
    20761 ttgttattaa cgacagaagc agtcgttgca gataaaccag aaccagcagc gccagcagct
    20821 cctgcaatgg atccatcaat gatgggcggt atgatgtaag aaaaagcaac ttgcttatct
    20881 acaactgatc attagtgaaa gacagataaa aagaggctgg aacagaagcg tttaactcca
    20941 agaaataaga agaaattcac ggaaattgct tttcaaattt ttgtgaattt cagcttattt
    21001 ccgaaggagt tgcttctgct tccgctgttt atacgtgttt agagcgtgag acaaaagtgt
    21061 tctttacttt tgtctcacgc tcttttttgt gtgtaattat aatgaaaata gttattttta
    21121 atagttataa tattttatct attaaaagta aatatccatt cactaataat aaagatatat
    21181 ttacagtatc attataatgt attcaattta aaataaga
//
LOCUS       DACCDF010000044        21071 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCDF010000044 DACCDF010000000
VERSION     DACCDF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.045"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..570
                     /locus_tag="IVK51_001659"
     CDS             <1..570
                     /locus_tag="IVK51_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6431257.1"
                     /translation="APGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(994..1164)
                     /locus_tag="IVK51_001660"
     CDS             complement(994..1164)
                     /locus_tag="IVK51_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6431258.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1358..1537)
                     /locus_tag="IVK51_001661"
     CDS             complement(1358..1537)
                     /locus_tag="IVK51_001661"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431259.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1547..1771)
                     /locus_tag="IVK51_001662"
     CDS             complement(1547..1771)
                     /locus_tag="IVK51_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431260.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1924..3036
                     /locus_tag="IVK51_001663"
     CDS             1924..3036
                     /locus_tag="IVK51_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6431261.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3114..3407)
                     /locus_tag="IVK51_001664"
     CDS             complement(3114..3407)
                     /locus_tag="IVK51_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP6431262.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3575..3889
                     /locus_tag="IVK51_001665"
     CDS             3575..3889
                     /locus_tag="IVK51_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP6431263.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3910..5889
                     /locus_tag="IVK51_001666"
     CDS             3910..5889
                     /locus_tag="IVK51_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6431264.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5902..6228
                     /locus_tag="IVK51_001667"
     CDS             5902..6228
                     /locus_tag="IVK51_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP6431265.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6244..6762
                     /locus_tag="IVK51_001668"
     CDS             6244..6762
                     /locus_tag="IVK51_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431266.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6762..8114
                     /gene="celB"
                     /locus_tag="IVK51_001669"
     CDS             6762..8114
                     /gene="celB"
                     /locus_tag="IVK51_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6431267.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8198..9703)
                     /locus_tag="IVK51_001670"
     CDS             complement(8198..9703)
                     /locus_tag="IVK51_001670"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP6431268.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9795..10385)
                     /locus_tag="IVK51_001671"
     CDS             complement(9795..10385)
                     /locus_tag="IVK51_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6431269.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10564..12210
                     /locus_tag="IVK51_001672"
     CDS             10564..12210
                     /locus_tag="IVK51_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6431270.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12259..12978
                     /locus_tag="IVK51_001673"
     CDS             12259..12978
                     /locus_tag="IVK51_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6431271.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13457..14404
                     /locus_tag="IVK51_001674"
     CDS             13457..14404
                     /locus_tag="IVK51_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6431272.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14416..15336
                     /locus_tag="IVK51_001675"
     CDS             14416..15336
                     /locus_tag="IVK51_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6431273.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15377..16834
                     /locus_tag="IVK51_001676"
     CDS             15377..16834
                     /locus_tag="IVK51_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP6431274.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17179..17793
                     /locus_tag="IVK51_001677"
     CDS             17179..17793
                     /locus_tag="IVK51_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6431275.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYSSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17786..19510
                     /locus_tag="IVK51_001678"
     CDS             17786..19510
                     /locus_tag="IVK51_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP6431276.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19507..20958
                     /locus_tag="IVK51_001679"
     CDS             19507..20958
                     /locus_tag="IVK51_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431277.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6796 a         3564 c         4245 g         6466 t
ORIGIN      
        1 gcaccaggag tgtatcagct ggtagaaacc caagcgccaa cggggtatga attggatgcg
       61 acaccaatcg agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa
      121 aatcgactta caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggagaagtc
      181 ctgcaaggag ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta
      241 accacaggag cagatggaaa actggcgata gatggcttag caccaggagc atatcagctg
      301 gtagaaactc aagcgccaac ggggtatgaa ttggatgcga caccagtaac tttcaagatc
      361 gaaaaagaac aagaagtggc gatcttttta acaaaagaga atagaaaagt tgctgatagc
      421 tcagatataa gaggaaacaa tccaacaagc agtggaaata agcatcttcc aaaaactgga
      481 gaaaccatca ttagccagtt tatattgtca atattgggtg tgctacttgt tttcatcagt
      541 ataaaattta gaaagaaacg aaatatatga gctaaaataa aaaattgaat aagcatagtt
      601 tttaaaatca actagtaccc atcaattgaa tggaaactac cgattttaga aggggtatac
      661 aaacaaatat tagtatcgga gtagagaagg ttgaacccaa ccagtaaata agtaaagaca
      721 attttgtgct gcgttgagaa gtgaaaaata aacctttgta gtaatactag ttaataaaaa
      781 atatacaaaa acactgcgaa aatcattctt gagtttcgca gtgttttttg cctaatgact
      841 caagtttatt gggtaatact ttgattttct ttctacaaat aatcagcgat cagttgtaat
      901 tttttctcta gttttaatgg ctctttgtca ataaggagaa aaacaagtcg ggtcttttgt
      961 ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc tccttgaaag gcgtggaaaa
     1021 accaagtaat cgatcaatca ccaatttcgc atttggtaga acatgtttgg tgagctgaaa
     1081 gtaggtggcg ttcatgtctg taactaagaa ttctatttct ttaggattag tgcagctaag
     1141 gaaatagttt gttaatcgag gaattttttg tgtaggtaaa ttttctcgat taatttttct
     1201 gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa cggaaacgtg agaacgaaat
     1261 tcacaaccct caacactcaa gacagaattc ttttggaaga ctttggtaaa tagcttctca
     1321 actctttcaa tgtgcgaata acggttgtca aagataccta acaacttttc gaaataaaaa
     1381 ataacgacac tttttcagtc agtaaagaag tgattttatg tctgacatga tttgaaatcg
     1441 agtgccttgg acggataaaa taactttgag cgttccaatg gcttcgacag tttttacagg
     1501 tatagcgctg cttttttagg cgtataacca ttggcatatg attatattat tcaaagtgga
     1561 caatggtttc cttcttcttg tttttcacca ccattaattt cctatttcca tctaccacag
     1621 cagaaccaca atttctacag acacatggag cagggaagag aactgcagca acgatcaatg
     1681 tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat catcaaattt ttgtctattg
     1741 atctcagaat ttttttgata gaaccattca taaagcatat cgttctctca gttgtttatt
     1801 ttgtggtgat ttaatcatac aagaaaacga tatgtttttt tataaaaaca tgaataggaa
     1861 aaatagttga caagaaaatg acaacgccat atgatgttcc atagtattta caacctgtca
     1921 tagatgactg taaaaaataa aagtaaaaag aaaggacgac aacaggtgga tttttatcag
     1981 tctctacaac tagatccgtt tattttaaaa caaaaaatac gagaagcagc ttctaaaaaa
     2041 gaaaagtgca tgtatatttg ttcattgttt ttacgatcat tattcatcgt attgtttgcc
     2101 atttgtttta tcattataat tacaacatta tttgaatcga agcataaacc atacgctgtg
     2161 gttttattct gtatgttgat gagtattcgt tttgttgact ttggttataa gatttcccat
     2221 tcgatcatag gtttagcaat cgtcatgttc tcactattag tagcaccgta tgtccagctg
     2281 atcaaatggt cagttatggg catgttgatt cattttattt tactaagcag catattaatg
     2341 gcaaccgcta gtgatcccaa aatggggaat gcttctttgt acggcttttc atatctcttt
     2401 attgtttatt cgttacctaa agattcgttg aacaagaatt tctttacaca aacaagtagc
     2461 ttattatttt tgtttttttg ttggttttca gttctccttt acagaaaaca tcgagaaaag
     2521 aacaaagaca aaagcttgtt taaagagatt tttttgaaag gcatgtattc gcaggaaaaa
     2581 atttggatgc tcatctatgc atttggcatt agtcttttga tcgttgcagg agagtatgtc
     2641 ccttttcagc gattgatgtg ggcagggttt gcattttctt ctattgtatc tagctatgga
     2701 ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa tcattggctc attgattgga
     2761 tgtgttttat ttattggtat cagccaattt attcctttta actgggtcgg tatattaggt
     2821 ggtctagctt taggtgtttg ttctacctat agatataaaa cagtttttaa ttcttttggt
     2881 gctttggcaa tcactgccag tttattcggg gttccaggag cagtaacgat ccgcattttt
     2941 gagaatatcc ttggggtatg tttaggaatc atgtatattg gggtgactga aatacttatc
     3001 cgaaaaataa gagaaaaaca cggtttgaat cattagatga gatcgaataa aaaataagga
     3061 aaaagtgaag atacacatgg atgataaatt gctatcattc catgtgtatc tttttacggt
     3121 aaatcagtga aacgttcgac tttcattttt aatttatact ttttacggag ttcgtttatt
     3181 tgagccatca tttcggattt gtgatgcttt tcgattgctt cttcatcttt ccaacggtcg
     3241 atcagtaaaa gagattctgg atcttctagt ggaaagtaat agtcatattt ctcgtttcct
     3301 tcttctgcac gaatcgcttt gacaatgttt ctttcgacca tttcttctgc aaattttctg
     3361 gcttgtccat ttgtacttgt atacaaaata ttcactgtga tactcactaa atcccctcct
     3421 gaaaaaattc tatcactagt atatcacggt gccagaaatt attattcaag atatgataca
     3481 cattttttcc gcaatgctac ggaaatatat attttgaata gaaaacgctt acaagatatg
     3541 cttggtttat aaaaataaaa ggagtgattg atttatgaaa aaagcattga ttatttgtgc
     3601 ggcaggaatg tcatcctcga tgatggcctc aaagacaacg gatttcttta agcaaaaaag
     3661 agaagaggta ctcgtagatg cagtttcagc tacagaaggc gataatatga ttaagaatag
     3721 tgattttgat ttatttctga tcagcccgca aacgacgatg tttttggata aattcaaaaa
     3781 attaggagaa tctgttggaa agcctgttgt tagtatccct ttccaagcgt atgtcccaat
     3841 ccctacaggc atccagaagt tagcagaggt gatcgaagag aacatatgat ggaataggag
     3901 tggatgataa tgaaacagaa ggaaaaattg ctgttgagat atttgattga tcacaagaag
     3961 gagtatgtca ccagtcaacg tttagcttct gaattgttat tatctgatcg gacaatccgt
     4021 aattacttgc agcggatcaa agagttagta gaaaaaaacg gaggtaagat cattgctaaa
     4081 ccaggttatg gctaccaact gcatatttta caaagactga catttgactt gtttttgagt
     4141 cggcaagaaa tagtacctgg atatgctaga gaaccgcaag gattttatga gtccgaagat
     4201 cgacaaaagt atatattgaa ccaattgctt ttggaagatg ccattctttt tatggatgat
     4261 ttggcagaag agctttatat cagtcgttcc agtttgacaa aggacatgca agaaatcaag
     4321 gagcgattga ttccatattc actaaaaatc gtttctaagc atggtcaagg gacatggatc
     4381 gaaggggaag aaagaaatcg acggcatttt atcatggata ctttttttgg aaaccattat
     4441 ggaaattcat tgaaagaata tttagggcaa agccgttttt tcccagacat cagttttgaa
     4501 gagctagtca ttatcattct cgatgaaaca agagaagcta agttaaaagt ttcggatttt
     4561 atcatacaga atctgacttt acacttggct ttggggatca aacggctccg agaagggttt
     4621 gaaatcaagg aattaggcat cgaacaagaa gtttctaagc gagtagagta tcaagtggcg
     4681 cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga agaagaaatt
     4741 gcttatttga cattacatct catggctaag tcgaatcaca aagaaaatca aaatgatcag
     4801 gaactatcaa aggaattagc gaaagttctg aaaagattgt ctcaggaatt tcatttttca
     4861 ttagtagagg atctccagct tttcaatgga ctactcgatc atttgtcacc gatgatgatt
     4921 cgattgagcc gaggtattcg cattgagaac ccattgactg aggagataaa gaaagaaaat
     4981 cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt gaaaaactat
     5041 accatcaatg aagatgaatg ggcctatttg gctttgcatc tcatggcggc attggaaaaa
     5101 gaaagagctg cgcataaact acatgcattg attatctgtg caacgggtta cggcagtgcc
     5161 caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt ggtaagtgta
     5221 aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat tatttcttct
     5281 attgatcttt cgacaatgtt tttcaaaatt ccagttttac atgtcagtat ttttttgaat
     5341 gaagatgatg ttcgtaaaat acgaaaggta gttggagaat ctatacccta tcattcctca
     5401 gatagaccga taccagcatt ttctctacag caaaaaaagc agatttacac cgaacagttg
     5461 tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat tcttcatgat
     5521 ttgctgcaaa agctgagcat aaatgaagaa gaaggatata taagagaaat gcgaaagcag
     5581 atccagcaac gtgaaaatat gggacaaatc atattcagtg atacagtggc agtcccacat
     5641 cccgctgttc cagttggaat cgaaacaaag gtagccgttg cattgattcc ttctgggatg
     5701 cagtgggatc aatatcaaga aattcatttt gtttttttag tttcgccttc ttatgtcgaa
     5761 aatgaaggaa ttacggtcgt gactaaagca atcgttaggt tagttgacca gctaaaagta
     5821 cagcaggaaa tcttagctga acctattttt gagaatttca gcagtaaatt tatcaaactg
     5881 atcgaataag gaggaaaaca gatggctgaa aaaataaaca gtgaaacact acaagtgact
     5941 tcttttgata tcattttcca tagcggaaat gcacggacac tgatacatga agcatttcgc
     6001 tcgatgcgta aagagatgtt tgaagaagta gaagagaagt tgcgacaagc aaatgaagag
     6061 attcttcatg cgcatcagtc acaaactcaa cttctgaaag aatatgccag cgggacaaaa
     6121 atagaaatgg aaattatcat ggtccatgca caagatcatc tgatgacgac gatgacatta
     6181 ttggaagttg cacaggaaat gaattatttg tatcaaaaaa ataaatagaa aggaaggatc
     6241 attgtgcagg atacagtaaa aaggaaaatc gaattagaaa aaaagcaact tagtttgaaa
     6301 aacatgtatt tcaatcgtta tctaatcgta cgttacctga ctgccttttt ctttttcatg
     6361 aacatgcaat ggatgattct gcttctatca gctaaaagtt taggaagtag tttaccgatg
     6421 attctgattt tagctatttt gcctgcagta ggagaacaag taaagcttta tcgaaaacac
     6481 caaacgaacg ttccatggac aaagtggtat tttttgttcc agagtgtctt taatttatta
     6541 gtgattccag tgttgtttac ctcaggattt acattcttgt atccttttat ggcaaataac
     6601 aatggaggac agttatttgt tttcattctt gttgtaagcg gtatttttgc cagtgcattt
     6661 atacagtatc gtttaaagaa aatttcactc aaccaagacc aacagtatat ccgtattaaa
     6721 caatatgaaa aagcgttata tttaggaaag gagaataact aatgtttgat tttttgcaga
     6781 aatacctgat gggacctatg gggaaaattg cgcaacttaa aatcgttcgg gctgtcatgg
     6841 cagcggggat ggcttctatt cctttcacga ttgtaggctc gatgtttttg gttttaaata
     6901 ttttgccatt accgttccca tttttagaag gattttttaa tgcaactttt ttcaaagtta
     6961 gtgacttgta catgatcgtc aatacaatga cgatggggat tttatctatt tactttgcca
     7021 ttgtgtttgc ttatgaatta acgtcaattg aacgagatga acaaaaatta aatgttaatc
     7081 cgctcacggg ggcattacta tctgtttttg cattctttat gtgtatccct gaattgatca
     7141 tgactgatgg taaaatcagc ttgattgctt ctatcaatga gacagaaact gtagtaaatg
     7201 gtattaggat gggctctttt gtcgaacgtt taggaacttc aggaatcttt acagctatta
     7261 ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt caagagaaat tgggtcataa
     7321 aaatgccaga tgttgttccg ccaggagttt ctcgttcttt cactgctctg atcccaacat
     7381 ttgtcattgc gtttgtggtg atgatcatca atggattatt agttgcacta ggaacggata
     7441 tcttcaaaat gattgctatc ccatttgggt ttgttacgca gttgacgaat acatggcttg
     7501 gtgtcatggt gatttatttc ttgatccatg cattgtggat cgtagggatc catggagcga
     7561 atatcatcac ttctttcttg accccgatcg tattagctaa tatggcagct aacgcacaag
     7621 gcgcaaatta tccgttggca ggagaattca acaattctta tgtaaccgtt ggtggatcag
     7681 gtgcgacttt aggattgatc atctttattg cattcctcgc gaaatctgat cagctgaaag
     7741 tgttaggaaa agcctcactt gtaccaggat tattcaatat caatgaacca atcattttcg
     7801 ggatgccaat cgtctacaat ccctatttag cggttccgtt tttcttagct ccgatggctt
     7861 ctgctacact cgcttatttt gccattcggc tggaaatcgt caaaccgatg cttgcacaga
     7921 tgccgtggcc ttcaccagtc gggatcggag catttgttgg tagtggtggt gattggaaag
     7981 ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat tatctggttc ccatttatca
     8041 aattctatga taataaatta ttaaatgaag aaagagaaaa ggcagcagaa ttagctagtg
     8101 aaaagttaaa atgatttcta agaaaaaagg aactgtgaca aaaaatcaca gtcccttttt
     8161 ttcttagaaa tgggaaacca ctagtatata aataccatta ttcttcaatc aaccgcttca
     8221 cgattgcata atccccttca taaggttcat ctacaccatt gattttttga tagggatcca
     8281 cgccttttcc cgcaataacg actgaatctt gcggagcgct ttctgctaaa gcgcgtcgaa
     8341 tcgcttcagg acgatccatt tcataatgaa cgatcaaatc tggattagta attgcttcat
     8401 tgatttcttc cgcgattttt ttcggatctt caaatgctgg gtcatctgcc gtcaaaaagg
     8461 cgatgtcagc tgtttctgat agcacttttc caaaatcatg tcgacgggag attgctttgt
     8521 ttcctgggct gcctaataca acaattacac ggccatctgg atgttcattt ttggcaaatt
     8581 tcagcaatgt tttcaagctt agataattat gtgcatagtc cacatagaca tgcgctccat
     8641 tggattggat caattgatcc attcggcctg gtacacgagc ttcttttaat ccgtcttgtg
     8701 catcttgact ggaggcacct gttagtgctg cggcaatgat cgcactggct gcattttctt
     8761 ggttgaatcc tccagccagc aaaatatcat aagcagtatc gcagatagct aagcgatctt
     8821 catgagaaga taaagtaaaa ccgtgagtac ctttatctga acggatcact tgataattgc
     8881 tttcttccga acgaccataa gtgatcgttg gaatctggaa tagatcggct gtttccctaa
     8941 gtaaatcaaa atagtcactt tcatgattta aaacaactgt ttttgaattc aagatcaatt
     9001 gtcgcttgca atagaaatag tcatcaaatg tcggatgttc gatcggactg atatggtcag
     9061 gcgagatatt caagaagatt ccgacatcta aagtcaagcc gtatacgcgt tgtgttttgt
     9121 aagcttgtga agaaacttcc atcactagat gagtcatacc attttcaact gcttctgaca
     9181 tcatacgata taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa tatgttttgc
     9241 catctaacgt tgtattcata gtagaaagca atgctgtttt tttatttgtt gtgtgatcca
     9301 agattgcttt tgtaaagtac gcagcagttg ttttaccttt tgttccagtg aatccgatca
     9361 ctttcaattt attttgcgga tagtcgtaaa aagccatagc aagaagcgcc attgctttac
     9421 ggatatccgt tacgatgatt cctactgctg aattttcgta tggttgttct gatacataat
     9481 attgaatacc ctgttcgatt gctgaatcca aatattcttc tttgaaattc attcctttac
     9541 agaaaaacaa agtagaagca tcagcttttc ttgaatcata acttaactga agaaattcag
     9601 ctttttctgg tacatcaaga tgccagcctc ttgttgaaac aaattctttt aatagatttt
     9661 cttttaatag taaatgacgg atgtcttcta aagagaagct cattgattga tccctcgctt
     9721 tattcaattt cttggtttat tatagcacac tctttttaat atatcgtttg tttccgctat
     9781 gacaaaatcc agctttattc aaattttctt gacacagggg acaagcggat gagtattttc
     9841 ataaacaatg aactgactgt aatagatagg agaacgaata gtgtctccaa caatccaaaa
     9901 acataataat tcaaaaagac gataagacta ttggtaagta aaagtccttg cggcattgga
     9961 agccgaaaaa atttgtggaa aataatcggc ggtatcgtta taccgccatt tgaggccccg
    10021 actgtgtaca atgtgtaaac cccaagactg aacaaaagac ttcctgaaat cagtgaaaag
    10081 aaatgtgagg agatcatttc cacttttgga acaatctcta aaacaacagg aaaaagcaga
    10141 cttcccgcta cagtcttaaa aaatactgat tttcctaata ggacagcggc tagaagcagc
    10201 atcataagat tgatgctcca aacagtaact gagcgttgga tagaaaaggc cgtttcaact
    10261 aaatgtccga tggcgcccac tccggcggaa gctatatcgt gagggagcaa gaaccaattc
    10321 aatccaactg tgataaagaa aagacctaaa aatataacgg gtagtttttt tatttcttgt
    10381 gtcattgata tctcctataa atgttagcca taaaaggctg tatatttctt tactttacca
    10441 aaaaaacgaa accgaattca aaaaaacaat tgttcttata aaagttatat aaaatcgatt
    10501 gccagtttct gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt tggaatctga
    10561 ccgatggata atcaaagaaa accagcatta gatcaattaa caccgcaaga aaaaatggca
    10621 cgaggctctg cttggatgac cgccagtaat atcatttcaa gattgctagg cgctgtctat
    10681 atcattccct ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg attattcaat
    10741 atgggataca atatatatgc attgtttcta ctgatctcta cagcaggaat acctgctgcg
    10801 attgcaaaac aaactgcacg ctataattca ttgaatgaat atggcacttc acgacgatta
    10861 tttatccgtg cattacagat gatgggcggg ttaggcctgt tgtttgcttt gttcatgtac
    10921 atcgcctctc cttggctagc tcatgcttct ggtggcggag aagaactgat tccaacgatg
    10981 cgttctttga gtatcgctgt attggttttt ccttgtatga gtgtgatacg agggtatttg
    11041 caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca ggtggcacgt
    11101 attttttata tgctattggc tacattcatc atcatgaaag tcatggatgg tgactatgtg
    11161 acggcggtca cgcaatcaac tttcgcggca tttatcggta tgctagcaag tattcttgtc
    11221 cttttgtatt ttttgaagaa acatcaagcg tatacatcgg cgtttattca ctacagcgaa
    11281 aacaaaataa atattgcaac gaaggaattg ttgttagata ccgttaaaga agcgattccg
    11341 tttattattg tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt cacttttatg
    11401 aagatcatgt ctgcatccac cgagtattcc aatgcacagt tactggatct tttttcgatt
    11461 tttagtgcca atccagataa gttgacgatg gtagttattg cattggcaac atcgatcgcg
    11521 tctactgggt taccgttgat tacagaagct gttacgttga aagatcgagt aggtctggca
    11581 aaattgacaa gcggaaattt acagttgttt tcatttttta tgtttccggc gacatttggt
    11641 gttatgttac ttgcctatcc gctaaataca ttattttata caccggattc tctaggaagt
    11701 aatgtactga tccaagcaag ttttgtagga ctcttcctag gactatatat gctagtttcc
    11761 aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta attatttggt tattgggttt
    11821 ctagtgaaac tccttcttca atacccagcc attcgtttat ttgaagtata cggcccttta
    11881 ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga tcttaaagag aatccatgaa
    11941 gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt tattcttacc
    12001 ttgattatgc ttttggcttc gtggatcaca aagatgattt ttggtgcttt tttaaacgaa
    12061 gatagcaaat tccagtctct attgttgatt cttgtagtag caggtatagg ggctttagtg
    12121 tatggttatt tagctttgaa aatccgcttg gcagaaagac tactaggacc aggaatagcg
    12181 cgtttacgac ataaattaaa aataaaataa gtgaatcaga agagctagat tgatgctctt
    12241 ttgaaatggg ggaaacgcat gcgtttagat aagtttttag cagatatggg acttggcagc
    12301 cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg aagtcaatgg acaagtggtg
    12361 aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg ttacttattt aggagaaatc
    12421 attacgtatc aaaaagattt ttactacatt ttgcataaac ctgcaggagt catttctgct
    12481 actcaggata atcatgatca aaccgtaatc gatttattgg aagaccagga ttatcgggaa
    12541 gatctttttc cagtcggccg attagataaa gacacagagg gcctcttgat tttaacgaat
    12601 gacggggtgt ttgcccatca gttactatca cctaaaaaac atgtggagaa agaatatcta
    12661 gctgaggtta gcggtataat gacagaagaa gatatccgtt tatttgcaga aggactgatt
    12721 attgatggcg atgagcagac attgccggca aaactccaaa ttgatcaagt agatcaagag
    12781 acagaaactt caattatccg tctgattttg catgaaggaa agtttcacca agttaagcga
    12841 atggtcaaag cagtaggaaa agaagtctta tatttaaaaa gaatccgtat gggaaaattt
    12901 tggctgccta aagatttgaa taaagggcaa tatcgaccca tgaccaaaga agagctagaa
    12961 caagtgagag aaaaataatt gaatggatag ctaaaagaat aagcagagta gttgtagcgc
    13021 catctgaaaa gcagacttgt ccgtctgact ttcggatggc ttttttgtga aatttcggct
    13081 tatttccgta gaagctactt ctggaacacc gtttgttcgg aataagggac tgtgacaaga
    13141 cttttgttac agtccccatt gttccttgtg actcagagtt agatagtaag gacacgatct
    13201 ccctaatact caggccaaaa cgctgaattc acaacctctg ataaacggtg ttcaatcagc
    13261 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc ccgttttttt
    13321 ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg tttcattttc
    13381 cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt gcgaatgaat
    13441 cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta tgaatctatg
    13501 gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt
    13561 ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg
    13621 gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt
    13681 ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat
    13741 ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa
    13801 acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat
    13861 tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat
    13921 cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat
    13981 cttggtgttc attggtacat ggaaagggat tggttataac agcattattt actttgcatc
    14041 tgttatggga atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg
    14101 gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat
    14161 tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa
    14221 ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac
    14281 atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt
    14341 tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt
    14401 ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat
    14461 aagacggcta attttttctc taatataatg attgcactct ttgctatttc ctgtattttg
    14521 ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg
    14581 tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg
    14641 caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg
    14701 atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc
    14761 cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc
    14821 aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg
    14881 ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc
    14941 tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt
    15001 cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg
    15061 ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca
    15121 ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga
    15181 gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg
    15241 atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt
    15301 gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa
    15361 attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt
    15421 gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag
    15481 caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt
    15541 gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta
    15601 cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta
    15661 tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt
    15721 gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat
    15781 taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc
    15841 tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg
    15901 aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa
    15961 cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt
    16021 agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca
    16081 atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg
    16141 actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg
    16201 gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc
    16261 agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc
    16321 acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact
    16381 tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg
    16441 agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca
    16501 gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga
    16561 gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc
    16621 accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc
    16681 taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac
    16741 attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat
    16801 gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg
    16861 acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg
    16921 gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta
    16981 ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct
    17041 cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat
    17101 aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta
    17161 atgtttggag gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg
    17221 gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt
    17281 ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat
    17341 aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat
    17401 ctgctattta gtttattttt atctggtact gctttattga gttacaattt atattcatct
    17461 gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt tgccttgctc
    17521 tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac gatctctgtt
    17581 cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc ttttctaaaa
    17641 ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg attgatcctg
    17701 tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca ttggcgtgaa
    17761 gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt caaaaggaac
    17821 cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa cgatgactgc
    17881 taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag aaaacatgac
    17941 aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct tgagtaggat
    18001 catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga cctacttaac
    18061 aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta ccgatgaatt
    18121 agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac taagtgcgat
    18181 atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa acaaaggtgg
    18241 ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt tgcctatcac
    18301 acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag aactagatat
    18361 gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt ctggaacgac
    18421 aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg aaagacaaga
    18481 aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg atctggaaaa
    18541 aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag ctgtactaga
    18601 taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca ttggtatcat
    18661 tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat attcacagca
    18721 agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag agacgcggat
    18781 cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca acgaaatgtt
    18841 ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac agcaagacgc
    18901 acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata cattagaata
    18961 tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg tgtatgcgtt
    19021 ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa aagaagaact
    19081 ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc gagtagccta
    19141 tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg tgatccagcc
    19201 attagttgaa aattatttta aacacggtat tgattttact cgattcgata atgcgctaag
    19261 tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata atggaaaagg
    19321 gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta aagtggaact
    19381 tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct tcggcagcag
    19441 ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta ttacattcaa
    19501 ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat tttgaatggg
    19561 ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac agcagagaat
    19621 gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt aacggatgtg
    19681 acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga acgtcataat
    19741 tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg aggtatgcaa
    19801 ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt tgaaagcctc
    19861 aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca agaaatctat
    19921 caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag tgaagaagaa
    19981 ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact ggcaagaact
    20041 catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta ctatcaacga
    20101 aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt tgatggcttc
    20161 tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat cggggaagag
    20221 acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa tctacagttg
    20281 catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc agaaacaaaa
    20341 gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca attggaagtc
    20401 gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat gatgccggaa
    20461 gatatacgat attccagttt tttactgttt atggagattc aacgggaatt gattgatctg
    20521 gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac ggtgcaagaa
    20581 cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa gtacagtgac
    20641 aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact tacactcaaa
    20701 gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt caaaaaagaa
    20761 acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc gaaacaactg
    20821 cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta caacaacacc
    20881 acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga gtaccgagaa
    20941 aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata aaaaattgtc
    21001 tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt tcatgacctc
    21061 tcgataaacg g
//
LOCUS       DACCDF010000045        20664 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCDF010000045 DACCDF010000000
VERSION     DACCDF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.048"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            168..362
                     /locus_tag="IVK51_001680"
     CDS             168..362
                     /locus_tag="IVK51_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431278.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVK51_001681"
     CDS             631..930
                     /locus_tag="IVK51_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431279.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVK51_001682"
     CDS             complement(1027..1461)
                     /locus_tag="IVK51_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431280.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVK51_001683"
     CDS             complement(1693..2403)
                     /locus_tag="IVK51_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP6431281.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREVYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVK51_001684"
     CDS             complement(2393..3253)
                     /locus_tag="IVK51_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP6431282.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVK51_001685"
     CDS             complement(3258..3896)
                     /locus_tag="IVK51_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP6431283.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVK51_001686"
     CDS             complement(3911..4210)
                     /locus_tag="IVK51_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6431284.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVK51_001687"
     CDS             complement(4253..5737)
                     /locus_tag="IVK51_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP6431285.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVK51_001688"
     CDS             complement(5757..6218)
                     /locus_tag="IVK51_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431286.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVK51_001689"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVK51_001689"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP6431287.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVK51_001690"
     CDS             7521..8291
                     /locus_tag="IVK51_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6431288.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVK51_001691"
     CDS             complement(8564..9391)
                     /locus_tag="IVK51_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6431289.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVK51_001692"
     CDS             9959..11194
                     /locus_tag="IVK51_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP6431290.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVK51_001693"
     CDS             complement(12193..12453)
                     /locus_tag="IVK51_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP6431291.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVK51_001694"
     CDS             complement(12480..13781)
                     /locus_tag="IVK51_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6431292.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVK51_001695"
     CDS             complement(14073..14531)
                     /locus_tag="IVK51_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6431293.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVK51_001696"
     CDS             complement(14544..15869)
                     /locus_tag="IVK51_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP6431294.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVK51_001697"
     CDS             complement(15886..17298)
                     /locus_tag="IVK51_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431295.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVK51_001698"
     CDS             complement(17315..18079)
                     /locus_tag="IVK51_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP6431296.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVK51_001699"
     CDS             complement(18183..19058)
                     /locus_tag="IVK51_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6431297.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IVK51_001700"
     tRNA            complement(19313..19397)
                     /locus_tag="IVK51_001700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IVK51_001701"
     tRNA            complement(19415..19486)
                     /locus_tag="IVK51_001701"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IVK51_001702"
     tRNA            complement(19496..19568)
                     /locus_tag="IVK51_001702"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IVK51_001703"
     tRNA            complement(19630..19714)
                     /locus_tag="IVK51_001703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IVK51_001704"
     tRNA            complement(19735..19805)
                     /locus_tag="IVK51_001704"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IVK51_001705"
     tRNA            complement(19851..19922)
                     /locus_tag="IVK51_001705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IVK51_001706"
     tRNA            complement(19934..20006)
                     /locus_tag="IVK51_001706"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IVK51_001707"
     tRNA            complement(20009..20082)
                     /locus_tag="IVK51_001707"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IVK51_001708"
     tRNA            complement(20089..20169)
                     /locus_tag="IVK51_001708"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IVK51_001709"
     tRNA            complement(20171..20246)
                     /locus_tag="IVK51_001709"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IVK51_001710"
     tRNA            complement(20282..20354)
                     /locus_tag="IVK51_001710"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IVK51_001711"
     tRNA            complement(20364..20436)
                     /locus_tag="IVK51_001711"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IVK51_001712"
     tRNA            complement(20452..20523)
                     /locus_tag="IVK51_001712"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IVK51_001713"
     tRNA            complement(20538..20627)
                     /locus_tag="IVK51_001713"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
BASE COUNT         6532 a         4319 c         3274 g         6539 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaaa cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa actgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacaccacgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgag
//
LOCUS       DACCDF010000046        20394 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCDF010000046 DACCDF010000000
VERSION     DACCDF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.049"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..111
                     /locus_tag="IVK51_001714"
     CDS             <1..111
                     /locus_tag="IVK51_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6431298.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(569..844)
                     /locus_tag="IVK51_001715"
     CDS             complement(569..844)
                     /locus_tag="IVK51_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431299.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            1298..1504
                     /locus_tag="IVK51_001716"
     CDS             1298..1504
                     /locus_tag="IVK51_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431300.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            1700..1867
                     /locus_tag="IVK51_001717"
     CDS             1700..1867
                     /locus_tag="IVK51_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6431301.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            1893..2237
                     /locus_tag="IVK51_001718"
     CDS             1893..2237
                     /locus_tag="IVK51_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6431302.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2242..2523
                     /locus_tag="IVK51_001719"
     CDS             2242..2523
                     /locus_tag="IVK51_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431303.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            2626..2940
                     /locus_tag="IVK51_001720"
     CDS             2626..2940
                     /locus_tag="IVK51_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP6431304.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2918..4612
                     /locus_tag="IVK51_001721"
     CDS             2918..4612
                     /locus_tag="IVK51_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6431305.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            4632..5810
                     /locus_tag="IVK51_001722"
     CDS             4632..5810
                     /locus_tag="IVK51_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6431306.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5773..6459
                     /locus_tag="IVK51_001723"
     CDS             5773..6459
                     /locus_tag="IVK51_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP6431307.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6459..7619
                     /locus_tag="IVK51_001724"
     CDS             6459..7619
                     /locus_tag="IVK51_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6431308.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            7629..8504
                     /locus_tag="IVK51_001725"
     CDS             7629..8504
                     /locus_tag="IVK51_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431309.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8501..8812
                     /locus_tag="IVK51_001726"
     CDS             8501..8812
                     /locus_tag="IVK51_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431310.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8802..9026
                     /locus_tag="IVK51_001727"
     CDS             8802..9026
                     /locus_tag="IVK51_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431311.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELVHPIAKKFNKDQYFIV
                     IDSMRYNVIYADYDNFYIYFYLESVGEYGD"
     gene            9016..9417
                     /locus_tag="IVK51_001728"
     CDS             9016..9417
                     /locus_tag="IVK51_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431312.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9410..9814
                     /locus_tag="IVK51_001729"
     CDS             9410..9814
                     /locus_tag="IVK51_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431313.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9826..10434
                     /locus_tag="IVK51_001730"
     CDS             9826..10434
                     /locus_tag="IVK51_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431314.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10454..10816
                     /locus_tag="IVK51_001731"
     CDS             10454..10816
                     /locus_tag="IVK51_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431315.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10819..11001
                     /locus_tag="IVK51_001732"
     CDS             10819..11001
                     /locus_tag="IVK51_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431316.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11018..14449
                     /locus_tag="IVK51_001733"
     CDS             11018..14449
                     /locus_tag="IVK51_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6431317.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            14500..15237
                     /locus_tag="IVK51_001734"
     CDS             14500..15237
                     /locus_tag="IVK51_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431318.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            15247..17538
                     /locus_tag="IVK51_001735"
     CDS             15247..17538
                     /locus_tag="IVK51_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431319.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            17562..19688
                     /locus_tag="IVK51_001736"
     CDS             17562..19688
                     /locus_tag="IVK51_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431320.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19851..20297
                     /locus_tag="IVK51_001737"
     CDS             19851..20297
                     /locus_tag="IVK51_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431321.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20299..>20394
                     /locus_tag="IVK51_001738"
     CDS             20299..>20394
                     /locus_tag="IVK51_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6431322.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT         7175 a         3066 c         4265 g         5888 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcata taaacacgga aaaataattg cttataaata atttggcggt
      121 tttttggcgg aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa
      181 agattaggaa acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa
      241 acttcttctt agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc
      301 taaattcttc atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca
      361 tgttcttgtc gtctttcaac tgctgctgtc tattaattta tgtattggag ggaagaaaaa
      421 tgaaattatc agtagaaggc actccagaag aaatacaaga attgctccaa gctattggta
      481 gtagcaagga gcaaaaggta agtgtttcta attcagatat tgacggtatt tgcaattgtt
      541 taaacagaga aaaaatatct aatccttact aagagaaaat atgttcgtta atgaatttcc
      601 aattttcctc tgtagctcta ccttgataat ttttttgcag ttcacagatg aataaggaat
      661 cgttattatc aagtactgta tttaattttt cacacatttg atttggattc aaattagttt
      721 taactaacca aaaagatttt tgtagcttta tatatgcccc aaattctttt attatttcaa
      781 atactttatc atacttttgc cctgggttat taagatcata actaatcatg tagggtttat
      841 acatgttgtc ttgcacatcc ttgttttata tttcagcgga ccactcgctg ataaataaaa
      901 ttatacgctt agtatttatt ttcacaatat taatttatcg taataacttt tgagtaaata
      961 gaacaaaaaa cctgcactag tttccgctag tacaggcagt gactatatcg gttgataatc
     1021 tagcatatat taaaaataat tgcaagaaaa gaaatttata gtggtttata ccaaattatc
     1081 aaagcaatgt tattttgttg ctgtctattg ttttttaatt attcacacga taactaaagg
     1141 tgggtgaaga gaaatgattc cattaataat ttcaattttt gcgctctgtc ttaatgtcta
     1201 tatgattgga tttaaaaatg ggcaaaataa aaaatagtag cagccaagaa taattttata
     1261 gtgtcactgt ggcggaaagg gtagacgcta agcatgtgtg ctaggtcaat gcttcggcaa
     1321 ccatgcaagg ttcgattcct tgccagcgac attaaatgcc tatgacggtt acgactaccg
     1381 aaaaaagatc gttaagaagc tatacggtgc tacgtacggc aatgtagtaa gtgtgctatc
     1441 tgtacaccac caagcttcgg tcactgtggc ggaagtagaa gacgcagcgg taaatggcga
     1501 gtagcctcgt gagagcctgg taagttctcg tgagtggtgc aatcccactc cagcgacttt
     1561 aagcaaccga ggcatcggcg gtttaaaaat ataggggtgc gcaatttcgt acgcgttttg
     1621 tgcatcgtgc aagttactat tacatattag atcactcttt gagtggtctt tttatttttg
     1681 cacaaaggag gaaacaacaa tgtatagacc acaatactta gaacagaagt atgaagtaat
     1741 cactgttcat aaaggtaatg gcgaaaaagt atatgagtat agaagaccaa taaagagcga
     1801 tacatataaa cgaaaggaaa acaatgaagt tattccattg tatggcaaaa gaatagctaa
     1861 gcattaaata agattgcgaa aggagacgga acatgaccga ggaattctat agatggctat
     1921 tacagttgac aagagaagat cgtttggtta agttctatca gtctcctaaa tggcgcaggc
     1981 ttagagagaa agcgatgaaa cgagatcact atgaatgcca agagtgtaga agactaggta
     2041 agtatcatag agtagagaac gttcatcata taaaggaagt caaggataga cctgacttag
     2101 ctttagattt agataatctt atttgtttat gtgttgaaca tcataatgaa gttcatggca
     2161 gatatcttac agcattagat aaacaagaga agaagataga aagctttgct aacttcgatg
     2221 caagtgaaag gtggtaagtg catgatcatc aatgataatg gcagagagta tgatacagaa
     2281 aagattgaag agtattcatc ttatactcag ggattaatta aacgtttgat atacgttcgc
     2341 tatgtaggta ttagggatct gttatcagat aactgttgta gtaaatacaa agtgaatcaa
     2401 gtaagagaag cgttgaataa agataataac gtcgaaagaa taaaaaatgt ttttggatat
     2461 agtattgaag agattaatta ttacattgac ttcgctgaag ctttcattcc gatggtgaga
     2521 taaccccccc ttaaaataaa tcgcaaattt tttgggggtg atgaaacgga gggggctgtc
     2581 aggaaaagag attttttcga actttatcat gaaaggaggg ctaaaatgtt taaaaacgaa
     2641 ttgtctcaaa atcgctacag agaaaaatta cgccgctctt taataagcca attggaaagt
     2701 cagaaaacaa atattgagcc attcttagat aatgttgatc gttatatcag tttatgggaa
     2761 acggcgatat cactggaaga agatatatcc gagaacggca ttagactgga gaatggtaaa
     2821 aagaatgaat cagtagcgtt gcttgtttct gtcaacaaac aaatgggatt gatgttggat
     2881 aaacttgcca ttactcctga attggtaggt gaagcaaatg aatcaattcc tgagttataa
     2941 gcatattgaa aattggttca aagctataga agaaggcact atcaaggtat gcaaagagca
     3001 attattgcta aaaaattatc tagaagaaag agtctttact agagaagata tttacttcga
     3061 taagcagatg gtagaggatt caatcaatat accagcacaa tactttccat tcgaattaat
     3121 tccgtgggaa aaatttctac aatgttttat ttatggtgtt cgatggaaaa aagataaaac
     3181 actagtgttc aatagatatc tttcattaat gggacgtggt aatggtaaaa ctggttttgc
     3241 ttcttggaac aacttctttc tactaaccgc taaacacggt attaaaaatt atgatattga
     3301 tatctatgcc aataatgaaa gccaagcaaa gactagtttt gatgatgtat ttaaagtaat
     3361 taaagatcat cctgatttag ataaaaaagt atttaaagct acgaaggaag ttattcaaaa
     3421 tatcgctaca aacagcaaac ttcgttataa cacggcaaat gctagaacaa aagatgggaa
     3481 gcgaccaggt gcaaaccgct ttgatgaaat tcacgaaaat gaagattatt caatgataaa
     3541 tgtggctact tctggtggtg gtaaaattcg agattataga gaattttatg atacaactaa
     3601 tggtcatgtt cgtggtggtc cgcttgatga cattatagaa gaatcaaaaa tgattctttc
     3661 tggagaactt ggaattgaca aggatggagc agaattttct agtttgtttc catttatttg
     3721 tcgcttggat aacgataatg aagttgatga tcccgacatg tgggaaaaag cttgtccaac
     3781 tattaattac aatgcagatt taaaacggaa aatgtttcaa gaatactctc aaatgcaacg
     3841 taatgctggt ttaagactta cgttcatgac caaacgaatg aacagaccta tggaagatac
     3901 acgatttgct gttgcttcat atgatgatgt tctgcatacg aaagaaaaag aatttcctga
     3961 aaaaatggat gaagtgatag gaacagtcga ttttgctgat agacgagatt ttgccagcgt
     4021 tgggttgcta ggaaaatacg ataaagatgt ttattttaca caacatactt ttatccacga
     4081 atcagccctt cgattacaaa acatcaaacg agaggttata gatatttcta tagatcaagg
     4141 aaaatcacag atcgttcatg gaaaaaatat agaagctgat tatattgtag gttggtttct
     4201 tgaaatgagt aataaatatt atattaaaaa aatcgctatg gatatgtacc gtgcaaaaat
     4261 attgaagccc gctttagaag aagcaggttt tactgtggaa attgttcgaa gcggatctgt
     4321 tacacatggt atgttaaaag atctggttga tgaccttttt attaatcaac gtttattttt
     4381 tggtgacgat gcgattatgc gttggtattg catgaatgta tatgaagagc atatttctaa
     4441 tggaaatata cgctatgaaa aaatagaacc tgaaactaga aaaacggatg gctttttttc
     4501 attccttcat ggtttgaatt ttttagatga tatttatgat tctgctcctg taacagtcac
     4561 aaatagctca gtagaaaata caggaactgg atttactcct ctagtattct aacttgaaag
     4621 gaggtgagaa agtggggatt tttcaaaagg cggtaggata cttcacaaaa aaagcaacgg
     4681 ttcctttaga agaatacttt tgtaaattgc aagttgattt tgtgtatcga aaatttgcga
     4741 ttgaaacttg tattgatttg attgcaaatg cgatgagtaa agcggaattc aagtcatatg
     4801 aagatggaaa aaataaaaag aatgatcttt actataggct gaatgtagct cctaataaga
     4861 aaaataatgc aacagaattt agaaaaaaac tgatcaggag attaatattc tacaatgaag
     4921 tattgatcgt ttctccgtct aataattcta gcgaaatatt tattgcggat agttgggatg
     4981 tcacagaata tgcattgaaa gatgatgtgt tttctcaagt gcaaattaac aacatagtcc
     5041 ttgatagaga atttctagaa agtgatgtta tctatataaa atacgcagat caacaaatta
     5101 ggcaactagt cgatgcgtat tatcaagcgt atgggaaact catttctagt gccatgaatg
     5161 tttacaagcg ctctaacgct cgtagatacg tactgaaagg gaatttattc cgaccgcaag
     5221 acaatacaac acaagatcaa atcaataaaa tgatgacatc acaatttaag gcttttatgg
     5281 aagctgataa tgcaggtgcg gtatttcaat tacaaaatga gtacacatta gaagatttca
     5341 gcggaaactt ccaaagcaat tcaagagata taaaaaactt aatagacgac atctttgaga
     5401 tgacagcagc agcgtttcac gttccgaaaa acctactaaa gggagacatg agtgggttat
     5461 cggatcaagt ggacgctttt ttaatgttcg aaatcatacc gattgctgaa cttattcagg
     5521 atgcgtttaa cgctagtctc tatgaagtag aagaatactt gtcagggaat tttgtacgtg
     5581 tggatacaac tatgatcaag attactagct tcaaagattt ggttgacgct attgatgtag
     5641 gcattagaaa tggagtattt acaatcaacg aaggaagaga acgcgttgga aatgatcgct
     5701 ctgataaggc gatggcagat gaaatattta taactaaaaa caatcaacaa gtatcgaaag
     5761 gaggtgaggc gaatgacgac aatgaaaaca tttctagcag taaagaatga aggcgcagta
     5821 ccgcaaattt ttattcaggg atttattggt tctagttggt tctttgaagg gaatactgac
     5881 aagggaatca aaaatatttt ggatagtcta ggtgatcaag aagaaattga agtagtaatt
     5941 aattcaaacg gtggagacgt atttcaaggg attgctattg ggaacttact taagtcaaat
     6001 aaagcaaaag ttaacgttgt gattaacggc ttagccgcta gtgctgcttc aattatcgca
     6061 atggctggcg atactataaa aatctacaac aatgcacaat tgatgattca ccgcgcttcc
     6121 acatacggag aaggaaatgt cgatgacttc cgtacgattg ctgaccaact ggaatcaatt
     6181 gataaatcgg taaaggcttc atataaaaca cgattcaatg gcacagatga agcattgcaa
     6241 gaacttcttg aaaaagaatc gtttatggat gcagaaacag ctttgagtta tggattggtc
     6301 gatgaaatta tcgatgcaga aaatagctca ggtactgaag ctaaaaaaga acaaagcgtt
     6361 gaagaaattt tgaatgacgt taaagaaaaa agagcagaaa aaattgctgc atttacagca
     6421 gcattaaata aaacatttgg acaaggagat gtaaaataat gacagttaaa aatttaaaag
     6481 gtgtaacagc tgcaagcgac caattgatga aagcttttaa agatggtaac gaagaatctt
     6541 ttagcgcagc tatggtaagc ttatctaagg aaattcagga taaaatttta gaagaagcaa
     6601 cagcaaaaaa tcaagatcaa ttagtattaa tgaaccgtgg tcagcgtgta ttaactacac
     6661 aagaaacaaa attctataac gaagtagtga aaaacgaagg ttttgcaggg gtcgaagaat
     6721 tagtgccagc tactgtattt gaacgtgtat ttgaagattt agaacaatct catccactat
     6781 tgcaaaaaat tacttttgtt aacacaactg gtgtaacaga atggattgtg tcacgtggag
     6841 tcaatccagc atggtggggt aaactttgcg aagctgttaa aaaagtttta gataatggct
     6901 ttgatgtaat taacatgaag cagttcaagc tatcaggtta tattcctgta tgtaaggcaa
     6961 tgcttgattt aggtccagta tggttagatc gttatgtccg tactgtttta gtagaatcat
     7021 tgagaattgc attagaacaa gcaattgttg atggtactgg taaagatatg ccagtcggaa
     7081 tgatgcgtga catgagcaaa caaactagcg gagaatatgc tgaaaaaaaa gcagaaccta
     7141 ttacagcttt agatgctgta actatgggcg gtttgatggc gcgactatca aaattcaata
     7201 tcgaaggtgt gaatgatccg atttatcgta atgtgaatcc ttctgatgtg gtcctaattg
     7261 tgaatccaac tgattactgg tctaaagtat tcccagctaa gactgtacta actgctaatg
     7321 gagaatacgt acaagtattg ccagtaccag tttcagattt gcagtcaacg gctgtgccag
     7381 aaggaaaagc agttattggg gtagcctcag attacttcat gggtgtagga tctacactaa
     7441 aaattgaagc ttcagatgaa taccattttg ttgaagacga acgcatttat ctagctaaac
     7501 aatatgcaaa cggtcaacct aaacgtaacg atagtttcat tgtattagat attagcgctt
     7561 tgggaactac tactacaact acaaaaccaa caaccacaac aactacaaca caagcgtagg
     7621 tgatcagaat gaagtatatt ctttgtcagc cggcaatcaa tcggtttaaa tgggagcttg
     7681 aagtttgttt aactaatctg aagaaactag gaatcaaaga tatcgtattg cttttcagca
     7741 gacacgatga tcagattcct attttttttg agaaggaata tggtgttgaa gttcatgtgt
     7801 acgatgatct gcgggacgac aaagagtata ttccttcgat taaaccatat ttatggtgga
     7861 aatatttaga agaagatcat tcgcgtgagg acgaccgata tttctatatc gattcggatg
     7921 tcattttcaa taaaagaatt aatttgcgca aattgccttc taaagatgat gtttggtatt
     7981 gtagcgactg ctgtagttat ctaagtcttg attatattag aagctgtgaa aacggagaaa
     8041 atattctaaa agatatggca aacattgtaa atgttacagt agaatctttg gaaactataa
     8101 acactaattc aggaggcgca cagtgggtta ttaaccgtcc taaagcgaat tattggaaaa
     8161 aggtttatct ggattctaat cggctatatc gctaccttag agggcaaaaa acaaatatac
     8221 aaatctggac agccgagatg tgggcacagc tttggaacat gatgtatttc aatattggtc
     8281 ctaaagttca cgaggaatta gacttttgtt ttgctactga tccaatagaa aaagttaaag
     8341 aagtaaaaat cttacacaat gctggagtaa caacaaacga tgaagattta tttttcaaag
     8401 ggagatacgt gacttctacg ccttttgatg aagatttatc atttgtaaac aagaaaaaat
     8461 gctcttacgc atatgttaaa gcaattaagg cggtggttag atgacgcctg aacaagtgac
     8521 tgaagaattg ctaatagctg tgaaggataa tatttacgtt acctggaacg aagaagatga
     8581 gtcaattaaa aagatgatag ctaaaaatgc tgtttatctt caaagtaaag tgagtacaac
     8641 actttctttt tctcctgaaa gcttagaata cggattgcta atcgaaagat gtagatacga
     8701 ctggaatcgt gctttagatg agtttgaaca aaatttcgct agtgagttat taggtttcat
     8761 tcaacattat gcgctacaag aatatattgc aggtgatgtg aatggcgaat aatcgtagac
     8821 tcgaagaaac attcaacgat ggttggttaa agattttgac gcaaactacc aaaagaaatg
     8881 aactagttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
     8941 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
     9001 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
     9061 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
     9121 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
     9181 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
     9241 gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt
     9301 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
     9361 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
     9421 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
     9481 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
     9541 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
     9601 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
     9661 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
     9721 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
     9781 tctgattcgt caattcgata aattaaataa atagggggaa caacaatggc taaaacaaaa
     9841 actgtagtaa caacgttcga taacgtaagt atcaaacgaa ttgcttttaa ttttaagaac
     9901 gcaggaaatg caatcgcaac gaattgtaac ggacaattag atggcgaaac agaaatgcaa
     9961 acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa aatctaaacc aatcaatatg
    10021 acggtaacaa ttactgcaca tgtaccgatg gaagtttatc gacgtttcaa tgggttgaaa
    10081 caagatggac gtattaaacc aggcatttac tcttacggtc ctgattccgt aggcgaagat
    10141 ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa atagaaagtt agttggtatg
    10201 ttagcatgca cttcgaatac aggattaaca ttctctattg aaaatggtgc ggatgaagta
    10261 gctgcgttag aactagaaac aaaagttatg caagatgaat ttggtaaatt ttatcatgaa
    10321 gcaattgttg cagaacttga agaagactta acagatcaat ggatgacgaa tctatctgct
    10381 gatgtgatta aaaaaacttc agttgtgaca actacggcca ctcaatcaca gtaaaaaaaa
    10441 cggaggtagc gaaatgaacg aagattactc aaaaattgaa ctaaacgatg gaacaatttt
    10501 gaatttagaa cctaaactga atatcaagaa attattgatg atcaatagag attttaacac
    10561 agacgagttt gcaaaaatga ctgtgggaaa aggatccatg gatatttctg ttattcaagg
    10621 tgcaaaggct gtgtatattg cttaccgcca agcgaacatg actgattata tttcattcga
    10681 tgaatttatc gataaatggg attttgatat ggctactgcc agctatattt atcaattgat
    10741 gatgttcaaa caagcacgcg atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa
    10801 aaagcttcaa aagtaaaaat tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg
    10861 atgttgaccg acgttttttc tatgccttca gatttggttt taagcgatat ctgtttagat
    10921 gacattttgc aaatggctta caacaagagt gcttatgaag gatggaaaaa ctatgcaata
    10981 aaccaatccc agaaaaacta aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc
    11041 agaagtaact ttcatagcta acgatgacgg attgaaatct acgttaaaag aaatcagtgc
    11101 tgaattaact aaaaatagag cagaattaaa actagaacaa gctcaattac aacagactgg
    11161 ttctgaatca gacaagttag gaagtaaatt atcttcttta gaaaagcagt atgaattaca
    11221 aagtcaaaaa gttgaagtaa ctagccaacg tttagccaat gcgaaaaaat attatggaga
    11281 aaattccacc gaagttaaga aacttgaaag agaactgatt aatcaacaaa cagcgcaaca
    11341 acgtttgtca aacgaaattg ataaaacgag taatgcacta gctcaagcaa aaggcgaaat
    11401 acagacgtac gagtctacaa tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag
    11461 tgcttctctg attgaatccg aatacaaaaa atggcaagca actgctggtc aatcagcttc
    11521 tgaagccgag aaattagcga aagcccaaga atatgtttct caacaatctg aaaatgcgga
    11581 gaaaacgata gatatcctaa gacgacagtt agaagctaca cagtctgaat ttggcgctac
    11641 atccacagaa gcaatgcaga tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga
    11701 gttaggacaa gctgctaaaa atgtagatac aactaacttg gacgatatcg gaagcaaaat
    11761 agatatgaat aatttaatgg aagcttctga cgttttaagc gacattggcg ataagcttac
    11821 agaattaggg aaacaagcag tggactctgc taacagtgta ggtagttccc agagtaagat
    11881 acaagctaat tttggtttgt ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga
    11941 catttattat aaaggttttg gagaatcgtt agatcagtcc acagatgcat tgattttggt
    12001 aaagcgtaat ttaggagatt taaataatca agatttacaa aatatcacgg aacaagctat
    12061 ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg ttacgtggtg taaatggctt
    12121 aatggtcaat ttcggcttga gtgctcaaga tgcaatggat ttaatggttt cgggtactca
    12181 aaacggttta gataaaacgc acgaattagg cgacaatatg gcagaatata gccaattatg
    12241 gagtcaaatg ggatattcag ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc
    12301 gggtgcttat aaccttgata aagtcaatga cttagttaag gaaatgggaa tatcgttaac
    12361 agatggtcga tttgagcaaa acatggatat gtttagtgaa agtactagaa aagcttttga
    12421 agagtggaaa aatggcggag gaacacaaaa agacgttatt aattccatga ttcaagattt
    12481 tagcaatatg gatggtcaat acgaccaatt aaataaagct tcgacaattt ggtctgcgct
    12541 tggcgaagat aatgcgatga aagttgtcca atctttaact gatgttaacc atacatttga
    12601 tgatgttagt ggatctgcac aaaaaatgaa tgaagattct actactccgt tgcaagagtt
    12661 gaacgggaaa atagctgaat taaaggattc attagctcct ataggcaaca caatcataga
    12721 tgcactcgaa ccagtaattg attttctagg aaagatggct gatgcgttta ataatcttcc
    12781 acaaccagta caggatttca ttgttgctat aggtggtctt acagcagcat ttggcatact
    12841 gttaccggca atactagccg tgtcatattt atttggtccg atgatgctta taattggagg
    12901 aattatagct gttatagcag gcgttattgt ggcaattaag aactggggtg caattactga
    12961 ctggtttagt ggtttatgga aaaaatttac tgattggttg ggtgatactt gggaaagtat
    13021 aaaagacgga gcctcatcag tttgggatgg ggttaaagaa acctggtctg gatttgtaga
    13081 ttgggttcaa gatatttggc aaggagtttc tgattggttt ggagagctat ggagcggatt
    13141 agttgaagga gcttccaaca tctggcaagg agtccaagaa acttggcaaa cattcgttga
    13201 ttgggtttca aatatttgga acggagtcaa agaagtatgg tcgattattt gggcagacat
    13261 tgtaggaatt gttcaaatac catggacatt aataacgtca ttgattcaag ctggtattaa
    13321 tattatcgtg ggtatttttg atgtagctgg acagttattg ggcgcagctt ggcaagctgt
    13381 ttggacacct atttctgatt tccttaaaaa tacttgggat actatgacac aatggataag
    13441 tatcgcttgg aatgggattg taactacatt ccatactata tttgatccag tagtggcatg
    13501 gtggaatggt atatggacag ctattagtac tacggcttca aatatttgga attcaattag
    13561 tgcaacagct tctagtattt ggaacagtat caagaataca atcactagct tggtacaagc
    13621 agctgctaca gtaattcaaa atatttggtc aactgtatct agttggttag gtggaatttg
    13681 gaattcaatc agctctacag catcaaatat ctggaatagt gtgactagta gtataagcaa
    13741 tgctataaac gcagctaaaa gtgccattca aagtgtttgg aatagtatat cttcgtggat
    13801 tagcggaatt tggaacggta tcaaaaatac tgctttgaat ctttggaatg gaattacaag
    13861 cactattagc tctaaagtaa acgatggaaa aaatgcaatt tcaagcggtt ggtccaatct
    13921 aacaggtatt gtttccgaca tattcaataa tgttaaaagt acaattgcta acatatggga
    13981 aggcatcaaa aagactgtta gcgctccaat tgattggatt agagacaaaa tcagtggcat
    14041 ttttgataat ttgaatattt ctataccaca tattccgtta ccggaattta tcatggaagg
    14101 cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt gtaaaatggc atgctaaagg
    14161 aggtatcttt actaaaccaa ctttactagg tggaatgaac ggtgtcggtg aagcaggacc
    14221 tgaagcagtt ttacctttga aaagatctgt tttgcaagaa attggtgatc gtatcttgag
    14281 tagcacatca gtttcatcta gggcacaaac gattcaacct gtgaataact acgaattcaa
    14341 tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc aacaaatcat
    14401 tgatagcatt acaaaaattc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    14461 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    14521 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    14581 ccttctccaa aaagacagag agttgaaact tctgttttag gtaggttagg tggcttagta
    14641 caagattact cgtttgaaga tatgtcgttt acattgcact ataactattt agaggatgtg
    14701 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    14761 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    14821 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    14881 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    14941 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    15001 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    15061 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    15121 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    15181 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    15241 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    15301 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    15361 agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa cggaactaat
    15421 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    15481 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    15541 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    15601 gaaaagatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa accagctatc
    15661 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaag
    15721 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggt
    15781 gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac ggatcataaa
    15841 atcaaagcga tttatactaa aaacttaact ggtcttgact tagagattga tgctcaaagt
    15901 gttttagttg gagtttatcc attcattagc agctcttcag aaggagaaga cgagatcact
    15961 ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    16021 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    16081 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    16141 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    16201 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    16261 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    16321 aacttcttag aaaacacaga gaataatgtc agcaatttga ttaatgatgc cattgatcaa
    16381 ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata ttgtagatca tcaaactgat
    16441 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    16501 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    16561 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc atggacgtta
    16621 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    16681 ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa tggcaaaatg
    16741 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    16801 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    16861 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    16921 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    16981 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    17041 actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt tggttcatgg
    17101 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    17161 gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg actattgaac
    17221 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgacaat
    17281 ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa cagtaaatct aagtcggtat
    17341 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    17401 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    17461 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    17521 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    17581 atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    17641 gtgataaagg gattacggtg acggttaaac cgtttctaaa tggcttgcag atggatacga
    17701 gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta gataatgttg
    17761 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    17821 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    17881 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    17941 atgtttcgca attagaagag ttgattcgaa agtacaacga aacttttgat gcttttatgg
    18001 ctgaaatcaa aggtagagtg gatagcttaa atcaacagat tactgattta actggtcaag
    18061 ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag ttaggtaact
    18121 tacaagtgat gtacagtaac agcatcgact tcggggacta tgattattcg gagaacccga
    18181 atttaatgcc atatattaca gaaccttggg ttggaccttt gttaggtaat ggacatacag
    18241 ttaaagatag tgttaaaagg gttataactc atactaaaac tagaacagct aattccggcg
    18301 acatattatc gcttggctta ggtattccgt gtacagcgga agctaataat aggtatctta
    18361 taaccacttt acgaccatct acgacataca cattaagcgt tactatgagt gtcggttcag
    18421 attggactgg agagaccaat actatagggg ttaggcttag atatttaaat gaacaaggtg
    18481 gtatagaact gcccattaat gcattaatac cagcaaatgt agaacgcgat aaaatggtca
    18541 ctcatacatt tactggtata acaaaagata atgtaactag cataacaaac tgttatgttg
    18601 aaatattctc acttaatagt gaatataaag gaacggttag tgtcagctat gacgttaagt
    18661 taaaagcaca ctaccctaac ttactagatg gcccttactg gttaggtaaa gttcctttag
    18721 gcgagaatat tgctgaccct acagttgtct ttccacacaa aactagtgaa tatatggttt
    18781 atggtagacg caatacagaa aactatatcg ctgaccaaac atataccata tcaatgaaag
    18841 caactaaact aacagttcaa tcgtttgccg tgtatattgc tgctggccgc gttaaagtag
    18901 gagacatgaa gcctactgag gggttagcta atacatggga gttaactttt actgtaacta
    18961 agcaacatat agatagtggg gtaactaatt atctggaaat ataccaatac ccgtcagcta
    19021 caaaaggagc tgttcaaatt gagtggctca aactagaaaa aggcaatact cgaaccccga
    19081 atattagtga gtataaatat cgtggtactg gtatgcgtga ttcaaacaat ccaaaagatt
    19141 atgtttggga tctagcacca gaatatgtcg aagataattt ggccacagat attaaaattt
    19201 ctgaaattac tggtaaagca aacaattata ccgatgggaa agtatcggag attaattcgc
    19261 agttgactgc ttcaattaat gaagtagaca ccacagctaa ggatgctcaa acaaaagcga
    19321 atgctaatgc gactgctata gatgaattag acaataagat cgatgaacgc attaatgata
    19381 cagctactac cacattaaca gttacaaacg ggaataccgg atcagcaaag ctttatcgtg
    19441 aaggaaaaac agtttctata tattttgtgg ctttaaacgg aaaaagcagt ggtggaaatg
    19501 attcaacgat actaacaatt ccagaaggct atcggccacc aattagtttt gagcaactgg
    19561 ttggctcgat agaccgttct actttgaaca gtgctcagtt atctattggt gcagatggag
    19621 ccattaaatg gcgaagaaac tcaagttatg gatcggatta tacctttgca attacttaca
    19681 cgatttagaa aagcgtgaat cgatatgaag gcagcatata gaccaattga accttacgga
    19741 ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg aagaatgcac agaagtcgaa
    19801 ccaccgattc caaattggaa accgaaattc aattactggg agggaaataa atgaaaaaca
    19861 tttggaaata tggacgtact ggcggagagt acgcaggaaa agtattggac gacatgcttg
    19921 tatccgttcc ttacacagat cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt
    19981 taacaatcgc tgatcaaatg tttgatccta aattgaatca atggattatt ttagcgaacg
    20041 cactagatca caacgattta aacaatctca aagcaatgta tgagtcgtta gaaaatgaga
    20101 acggcgattt aaaacagatc aatgccaaac tcatgctaag cgatgtagca attaaacagg
    20161 aaaatactgc attgaaagaa aaagcggata gtttagcaca aatcaattca aaaatgatgc
    20221 ttgcttcgtt acaaaatagc aaagacattt cagaaattaa agagcaacta aatccagctt
    20281 caaagggagg tgagtagtat gtttagtttt agcgatgtga aaatgatgta tgattggggc
    20341 tgtttcacta acgaacaagt aatggttttc gttccgttgt gcattactga agaa
//
LOCUS       DACCDF010000047        19963 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCDF010000047 DACCDF010000000
VERSION     DACCDF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.050"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            4..816
                     /locus_tag="IVK51_001739"
     CDS             4..816
                     /locus_tag="IVK51_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP6431323.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="IVK51_001740"
     CDS             813..1613
                     /locus_tag="IVK51_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP6431324.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="IVK51_001741"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="IVK51_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP6431325.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="IVK51_001742"
     CDS             3110..4819
                     /locus_tag="IVK51_001742"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP6431326.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9383
                     /locus_tag="IVK51_001743"
     CDS             5031..9383
                     /locus_tag="IVK51_001743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP6431327.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9527..10000
                     /gene="rimP"
                     /locus_tag="IVK51_001744"
     CDS             9527..10000
                     /gene="rimP"
                     /locus_tag="IVK51_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP6431328.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10024..11199
                     /gene="nusA"
                     /locus_tag="IVK51_001745"
     CDS             10024..11199
                     /gene="nusA"
                     /locus_tag="IVK51_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP6431329.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNSKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11221..11514
                     /locus_tag="IVK51_001746"
     CDS             11221..11514
                     /locus_tag="IVK51_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP6431330.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11511..11822
                     /locus_tag="IVK51_001747"
     CDS             11511..11822
                     /locus_tag="IVK51_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP6431331.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11835..14141
                     /gene="infB"
                     /locus_tag="IVK51_001748"
     CDS             11835..14141
                     /gene="infB"
                     /locus_tag="IVK51_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP6431332.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14168..14515
                     /gene="rbfA"
                     /locus_tag="IVK51_001749"
     CDS             14168..14515
                     /gene="rbfA"
                     /locus_tag="IVK51_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP6431333.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14623..14844)
                     /locus_tag="IVK51_001750"
     CDS             complement(14623..14844)
                     /locus_tag="IVK51_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6431334.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15292..17654
                     /locus_tag="IVK51_001751"
                     /pseudo
     CDS             15292..17654
                     /locus_tag="IVK51_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18022..18945
                     /gene="truB"
                     /locus_tag="IVK51_001752"
     CDS             18022..18945
                     /gene="truB"
                     /locus_tag="IVK51_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP6431335.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18949..19890
                     /gene="ribF"
                     /locus_tag="IVK51_001753"
     CDS             18949..19890
                     /gene="ribF"
                     /locus_tag="IVK51_001753"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP6431336.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         6843 a         3508 c         4387 g         5225 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgtgtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccagctagga cgaaatattc
     5701 cagccgatga accaacgaca ccaatgatca gtatcgttga agaagaacga agagtcacac
     5761 ttgaggggta cgtattcgac aaagaagtca gagaactacg ttcaaaacgc aagatcttga
     5821 ctttgaaaat cacagattat acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa
     5881 aagatgaaca agtatttgat gcgatcagtg tcggtagctg gttaaaggta cgaggaagta
     5941 ttcaagaaga tacgtttgtc cgtgatttgg tcatgaatgc gcaagatatc atagaagtca
     6001 aacatacacc tcgcaaagat tatgctcctg aaggggaaaa acgagtagag cttcatgttc
     6061 atagcaatat gagtacgatg gatgcaacaa acagcatctc tgatcttgtg gcacaagcag
     6121 gaaaatgggg acaccgagct atcgccatta cagaccatgg tggtgcgcaa gcctttccgg
     6181 aagcacatag tgctggaaaa aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg
     6241 tcgtagatga tggtgtccca attgcttata atgatgcaca tgaagcattg agtgaagcca
     6301 catacgtcgt atttgacgtg gaaacgacag gattgtctgc agtatatgac acgatcatcg
     6361 agctagccgc agtcaaaatg tataaaggaa atgtgatcga aagctttgat gaatttatcg
     6421 atccaggaca tccattgtca cgaacaacgg tagatctgac tggtatcacg gatgaaatgg
     6481 ttcgagggtc aaagtcggaa gaagaagtat tgcggatgtt ccttgaattt tcaaaagata
     6541 caattttggt agcccacaac gcggcttttg atatggggtt cttgaatact agctacgcaa
     6601 ggtatggtat tcctgaagcg gccaatcctg tcattgatac gttggaatta gctagatatc
     6661 tgtatcctca attcaaacgt ttcggattag gtgtgttatc taaaaagttt ggtgttagct
     6721 tggaacagca tcaccgagca atctatgatg ctgaagcaac tggtcactta gcatggatct
     6781 ttgtgaaaga ggcaatggac aatcataaca tgctttatca tgatcagcta aatgagcata
     6841 tcggagaagg agattcttac aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa
     6901 atcaggcagg actgaaagat ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg
     6961 aacgcgtccc acgaatacca cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg
     7021 gctcagcttg tgataaggga gaaatattcg aagccatgat gcaaaaaggg gtagaagagg
     7081 caagaaaccg agcaaaattc tatgattata ttgaagttat gccaaaagcg gtctatgcgc
     7141 ctttgatcga gcaagaattg gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt
     7201 tggtagaaat cggaaaaagt ttggataaaa tcgttgttgc aacaggtaat gtacattatc
     7261 tgaacgaaga agatgccatt taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc
     7321 cgttgaatcg tcatagtttg ccggatgtcc atttccgaac aaccgatgag atgctaactg
     7381 catttcactt tttaggggaa gaaacagcga aagagatcgt agtagaaaat acgaataaaa
     7441 tcgcagatat ctgcgaagaa gtgattccag tcaaagatga actttataca ccgaagattc
     7501 caggttcaga agatgaaata tcagaattga gttacacaaa agcaaaacaa atgtacggag
     7561 atccgttacc tgagatcatt caaaaacgat tagaaaaaga gttgaattct attaatggta
     7621 atggcttctc ggttatttat ctgatcgccc aaaaactagt gcataaaagt aatgaagatg
     7681 gctatctagt aggttcacgt ggctctgttg gctctagctt tgttgcgacg atgacaggaa
     7741 tcactgaagt taatccatta gcccctcatt attattgtcc agagtgccaa tattccgagt
     7801 tttttgaaga tggtacgtat ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat
     7861 gtggtgcacg cttaaacaaa gacggtcatg atattccatt tgagacattt ttaggattcc
     7921 atggagacaa agtgcccgat attgatttga acttctcagg tgattatcaa gcagaagccc
     7981 ataactatac gaaagtattg tttggcgaag actatgttta ccgtgccgga acgattggta
     8041 cagtcgccga caaaacagca tatggctatg taaaaggata cgaaagggac aataatctgc
     8101 agtttcgaag tgcagaagtc gacagattag caaaaggcgc tactggagtc aaacggacaa
     8161 ccggccagca tccagggggg atcatcgtta ttcccgatta tatggatgtc tatgatttta
     8221 cgccgatcca atatccagca gacgatcaaa attcagaatg gaaaacgact cattttgatt
     8281 tccactcgat tcacgataat gtattgaaat tagatattct agggcatgat gacccaactg
     8341 ttattcggat gcttcaagat ttatcaggga tcgacccaca aacgatccct acggatgacc
     8401 cggaagtgat gcggatattt gccggtcctg aagtacttgg agtaagtcag gaacaaattt
     8461 attccaaaac agggacatta ggtattccag aattcggcac tcgttttgtt cgagggatgt
     8521 tagaagaaac gcatcctaca acttttgcag aactactgca aatttccgga ctatctcatg
     8581 gtacagacgt atggttagga aatgcggaag aattgattcg acgaggagat gcgacactgg
     8641 cggaagtaat cggctgtcgt gatgatatta tggtttattt gatccatgct ggattagata
     8701 gcgggatggc atttaaaatc atggaaactg ttcgtaaagg acaatggaac aagatccctg
     8761 atgaattgcg agaaacctat ctatcggcga tgaaagaaaa taatgtgccc gactggtata
     8821 tcgattcttg ttcaaaaatc aaatatatgt ttccgaaagc ccatgctgca gcttacgtat
     8881 tgatggctct tcgtgtcgct tatttcaagg tttatttccc tattttgtat tactgtgcat
     8941 acttttctgt acgtgcagac gactttgatc tagtctccat gtgcaaagga aaagatgctg
     9001 tgaaacaagc aatgaaagaa atcacggaca agggattaga tgcttccgta aaagaaaaga
     9061 atcaactgac agtgctggag ttagccaatg aaatgctgga acgaggattc aaatttggaa
     9121 tgattgattt gtataaatcg gatgccgtta actttgttat tgaaggtgat acgttgatcg
     9181 caccatttag agctgtccca agtttgggaa ccaacgtggc gaaacaaatc gttgaagcaa
     9241 gaaaagacgg tccattttta tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa
     9301 cattgatcga atatatgaac gacaatggcg tattgaaaga tctaccagat gaaaatcaat
     9361 tatcgttatt cgatatgcta taagcatctg ttttcctgtc tgcttgtaat tttaagcaga
     9421 gtatggtata gttcttatag gtaatgatac taactgtgag tgagcggatt tttcgctcac
     9481 tctttttaat ggaattacgc acttaatttt taaggaggcg aaacttttga gtagcgtcgt
     9541 tgaaacagtc acagaaatgg tgacaccgat tttagatgaa caaaattttg aacttgtcga
     9601 agtcgaattt gtaaaagaag gaaaaagctg gttcttacga gtatttattg acaaagaagg
     9661 cggtatcgat atcgaagaat gcgcctttgt cagtgagaaa ctgagtgaaa aacttgatgc
     9721 agcagatcca gatccgattc ctcaagctta tttcctggag gtatcctccc cgggagcaga
     9781 gcggccattg aaaaaagaaa gcgactatga aaaggcactt ggtgattatg tccatgtctc
     9841 cttataccaa ccagtagatg gcgaaaaaca atatgaagga tttcttcaat catttgatgc
     9901 tgaacagctt acattgaaaa tacgcataaa aacacgagaa aaagaattga cttttgaccg
     9961 aaaaaatatt gcaaaagctc gcttagctat ccaattttaa tcacggagag gaatcagaaa
    10021 aaaatgagta aagaaatgtt aaatgcgtta gacgctttag aagctgaaaa agggatctca
    10081 aaagaaatcg tgatcgatgc attagaagca gcattggttt ctgcatacaa acgtcactat
    10141 ggccaagcac aaaacgtcga agtggaattt gatcagaaaa aaggaaagat ccatgtctat
    10201 gcggtaaaag aagtaacgga agaagtgatg gattcacagt tggaagtctc attgaaagat
    10261 gcacttttaa tcaatccagc atatgagatc ggtgatacga tccgttttga agtaacacca
    10321 aaagattttg gacggatcgc tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt
    10381 gaagcagaaa gaacgattat ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa
    10441 ggaatcgtcg aacgccagga taaacgttat atttatgtca atcttggtaa aattgaggca
    10501 gttctttcta aacaagatca aatgccaaat gaattttatc aaccacatga tcgaatcaaa
    10561 gtttatgttt cccgagtaga aaatacgtct aaaggtccgc aagtatttgt tagccgcagc
    10621 catccagacc tattgcgtcg cttatttgaa caagaagtac cagaagtcta tgatggaatc
    10681 gttgaaattg tcagcatcgc ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca
    10741 aatgatccaa atatcgatgc agtcggtaca tgtgtcgggc caaagggaca acgagttcaa
    10801 gcaatcgtca atgaattaaa aggcgaaaac atggatatcg tcgaatggaa tgaagatcca
    10861 gctgtattca tcgcaaatgc attgaatcct gcacaagtca tggatgtgat cttcgatgag
    10921 acaaattcaa aggcctgcac agttgtcgtt ccagactacc aattatcgct agcaatcggt
    10981 aaacgcggac aaaatgcacg tttagctgct aaattgacta accataaaat cgatatcaaa
    11041 tcagaatctg acatgacgga attttatgaa aatcaagaac aacaaaaaga aacagaagaa
    11101 ctgcatgatg aagcaatcgt tcagtcagat ttaacagacg atgaatatga aacaattgca
    11161 ttcaacaatg aaacggtcga agaaaaacca gaagcttaac tttagtaagg aggcaaagag
    11221 atgaaacaaa ggaaaattcc tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa
    11281 aaagagatgg tccgaatcac ccgttcaaaa gaaggggaag tatcgatcga tccaactgga
    11341 aaaatgcctg gacgaggagc gtatgtctca cttgagccgg aagaagtaca acaagcttgg
    11401 gataaaaaaa ttctcgatcg agtactcgaa acaacattaa cagatgagtt ttatcaagaa
    11461 cttcttgact atgtcactca ccaaaaagca cgtaaagagt tatttggaaa atgaatacag
    11521 ttaatcggca aaaagccatg aatctcatcg gtctagcgat gcgcgcggga aaactaatca
    11581 cgggtgaaga actgaccatt gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg
    11641 caaccgatgc aagtgagaat acaagaaaaa agataaaaga taagagttca tattatgaag
    11701 ttccttgctt tgagctattt tctgaagcag aaatcacgca gatgatcgga aaacctcgca
    11761 aggtattcgg aataatggat gccggatttg cgaagaagac caaggagcta attgaaggtt
    11821 aggaaggtga ttgcatgggc aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat
    11881 ctagtaaaga cgtcgtggaa aaagcacaac aacttggcat cgatgtgaaa aaccatatgg
    11941 gtgcgattac tagcagtgat gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga
    12001 aacaacaagc tgcacaacaa gcaagcagaa aaccagccaa tcaacagccg aacgatcaac
    12061 gtaaaaaaga aacaacgaac aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc
    12121 gcggtcaagg gagcggtcaa gtgaatcaag gaaaaaaacc aacaacaaac caaaataata
    12181 cgggaaatac cagcagccaa aatcgtcaag gaacgagcca aggcggcaat caaaaccgta
    12241 ctaacaataa caacaaacca aacaataaca atcgcggcaa atttaataat aataaccgta
    12301 atcgttataa taaaaaaggt aaaaaaggca aacagcaaca atcaactaaa ccagctgtac
    12361 caccacgcaa attccgtgaa ttgccagatg tattagagta tactgaaggt atgaacgtag
    12421 cagatattgc gaaaaaaatt catcgtgagc cagcagaaat cattaaaaaa ttatttatgc
    12481 ttggtgtcat ggtcaatcaa aaccaagcac tggataaaga tacgattgaa ctgttagcga
    12541 cggactatgg catggaacca caagaaaaag tccaagttga cattgctgat atcgataaat
    12601 tcttcgaggc agaagaagtc gatccagata aattagtctc acgtccgcca gtagtaacga
    12661 ttatgggaca cgtagaccac gggaaaacaa ccttattgga tacattgcgc cattcacgtg
    12721 taacaagcgg cgaagccggc ggaatcacac agcatatcgg tgcctaccag atcgatattg
    12781 atggtaaacc aatcactttc ttggatacac caggacatgc ggcctttaca agtatgcgtg
    12841 cacgtggagc aagtatcaca gatatcacga tcttagttgt tgctgcagat gatggcgtca
    12901 tgccacaaac agttgaagca atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg
    12961 ctgtaaataa aattgataaa ccaggtgcaa atcctcagca tgttatgcaa gaattaagtg
    13021 agtatgaatt aattcctgaa gcatggggtg gagaaacgat ttttgttgag atctcagcga
    13081 aattcggcca aaacatcgaa gaattgctgg aaatgatcct gttagtcgca gaagtagaag
    13141 atttaaaagc agatccaaca caacgcgcaa tcggaacagt gatcgaagca cgtcttgata
    13201 aaggtaaagg tcctgtgtca accttacttg tccaagaagg aacacttcac gttggtgacc
    13261 caatcgttgt cggtaatact tacggacgtg tccgtgtaat gacaaacgat cttggccgtc
    13321 gcgataaaga agcaggacct gcaacgccag tagaaatcac tggcttgaat gatgtacctc
    13381 aagcaggtga ccgtttcgta gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag
    13441 aacgcgcaaa acgtgcatta ttggaaaacc gtgctgccac aagccgtgtg actttggaca
    13501 acttatttga aagcctgaaa gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag
    13561 atgtacaagg ttcagcagaa gcactggctg catcattgaa gaagattgat gtggaaggtg
    13621 tccgtgtaaa aatcgtccat tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag
    13681 cagcagccag caacgctatt atcatcggtt tcaacgttcg tcctacaccg caggcaaaag
    13741 tgcaagctga tactgaagaa gtcgatattc gtcttcaccg tatcatctat aaagccatcg
    13801 aagaaatcga aactgcgatg aaagggatgc tagaccctga atttgaagaa aaaatcaccg
    13861 gtcaaatgat cgttcgtgaa accttcaaag tttctaaagt tggtacgatt gcaggtgcct
    13921 atgtaacaga aggatatatt cgtagagata gcggtgtccg tgtaatccgt gacggaatcg
    13981 taatctatga aggccaactt gcaagtctga aacgattcaa agatgacgtg aaagaagtca
    14041 aaatgggtta tgaatgtggt gcgatgattg aaaaattcaa tgacatcaaa gtagatgacg
    14101 taattgaagg atttatcatg gaagaagtca aaaatgacta aaaactgata aaaggaggcc
    14161 aatcattatg gctaactacc gtgatcgccg agtcggtcaa gaaatcttaa aagaagtcaa
    14221 tgatattttg cgcaaaaagg tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt
    14281 gaaagttacg ggggacttac agcaagcaac gatctattac agcattttat ctgacttagc
    14341 atcagataag aaaaaagcac aagatggatt gaataaagca tcaggattga ttcgtcgaga
    14401 gttagggcac aatctaagta tttataagac acctgaattg ttctttgaac ttgatgaatc
    14461 agttgtctat gggaaccata tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa
    14521 aaatcccgca aatcctgtga aatcaggggt tgcgggattt ttttgttttc attaagacgt
    14581 attacctaag agttccatga ctttagtctg gtagcgtgtc agctaaccaa aaaagagaaa
    14641 aagaaaagta aaaaattcga ctttcttttt tctatagtat ataaattttt gcatatagat
    14701 aaagatcaag ataccgataa cggaagctag attgtaatct attttagtga cagtcaggta
    14761 tagccaagta actagaagat cagtggaacc tgcaccataa aagtttgagt ttgtcggtcc
    14821 gttgacagtt aaaagaaaaa tcacgttgaa gttattgata tttccgatta cgccgcttcg
    14881 atttggtcac gatccaaatt ttggataatc cctgtagaaa ttagcatagt agctgggata
    14941 ccaattcaca tattgacaaa gataacagag acttttacat aaaacggatc aatcaggaaa
    15001 ggaattgctc gatccagccc cagttttcca acaatgtgtt aactggccta gcgtcattca
    15061 gcaaattatg catgatgagg agcgaaatga actgcggaac agccattgtg ataacgacaa
    15121 gttttttgga tcaatgtaaa aaacgtcagc gaaaggaaaa atttcgtggt tttttatacc
    15181 tgttcatttg gctagatata aaatccaaac aaatgaacag aagtgcttat taggtttcaa
    15241 ttgggagtat ctgcgctaga ataagagtaa taatgaaaag gagtgttatg tatggattat
    15301 tctagtaaag aatactttga taaaatgacg gcttggtggc gtgctgctaa ttatctttct
    15361 gttgggcaac tttatcttaa agataacccc ttactgagac gtacactgaa acccgaagat
    15421 gtcaaaaaac atccaatcgg tcattgggga acgattcctg gccaaaattt tatttatgtc
    15481 catttgaacc gagtcatcaa taaatatgat ttgaacatgt tttatattga agggccagga
    15541 cacggcggac aagtcatggt atcgaatgct tatttagatg gaagttacac agagatctat
    15601 ccagaagtaa cggaagatga gacaggaatg caaaaattat tcaaacgatt ttcatttcct
    15661 ggaggcattg cttctcatgc agcaccagaa acaccaggtt ctatccatga aggcggagag
    15721 cttggatact ctctttcaca tgcagtcgga gctgtattag acaatcctga agtaatctct
    15781 gctgtcgtga ttggtgatgg tgaagcagaa accggtcctt tagctggaag ctggttctca
    15841 aatgtcttta tcaatccagt gatagatgga gctgttttac cgatccttca tttaaatggt
    15901 gcaaaaatag ccaacccaac gatcttggca cgtaaatcag atggagaatt agcaaattac
    15961 tttaacggac ttggctggga acctttcttt attgaaggaa atgattcaga aaaattaaat
    16021 cctgttatgg ctgaaaaaat ggatcaagca atcgagaaaa ttaaatccat ccaaaaagaa
    16081 gctcgcctga agacagcaac agatgtcgtg atgccaaaat ggccagtatt gatcgtacgc
    16141 acaccaaaag gatggaccgg tccaaaagaa tgggacgggg aaccaattga aggaaccttc
    16201 cgagcacacc aagtaccgat tcctgtagat caagaacata tggatcatgc agatgcttta
    16261 ctccgttggc tgaaatccta tgaaccagaa aaattatttg atgcacaagg acgtatttta
    16321 gaagaaatca gagaaatcgc gcctactggt gaccaaagaa tggcaaaaaa tcctatcacc
    16381 aatggtggaa tcgatccaag accgcttatt atgccagatt ggaaaaaata tactttgcaa
    16441 tttgaaaaac caggctccgt taaagcagaa gatatgaccg aacttggtaa attcgttcgc
    16501 gagatcattg agaaaaatcc agaaaatttc cgtatatttg gaccagatga aacaaaatca
    16561 aaccgattga atcaagtttt caaaacgaca aatcgccaat ggatggaaaa aatcgaacca
    16621 gaaaatgatg aatggctttc gccatctggt cgcgtcatcg acagtcaatt atcggaacac
    16681 caagatgaag ggttcttaga agggtacgtt ctgactggac ggcatggttt tttgcaagtt
    16741 acgaatcatt cttgcgagtg gttgactcta tgttgactca gcacttcaaa tggatgcgga
    16801 aatcacatga cctttcttgg cgaaatgatt atccttcgct aaatctcatt gcaagctcaa
    16861 cagtcttcca gcaagatcac aatggatata gccaccaaga tccaggaata ttgacacatt
    16921 tggcagaaaa gaaggcggag ttcatccgtg aatatctgcc tgcggatgca aatacattgc
    16981 tcgcagtcat ggataaagca ttccgctcgt cagaaaaaat caatttgatc atctcttcca
    17041 aacatccgcg tgctcaattt tactcagcag aagaagcagc cgtcttagtg aatgaaggct
    17101 tgaaaatcat tgattgggct tcgacagcaa aagaggagga acctgaattg gtcattgctg
    17161 ctgcaggaac ggaatctaac ttagaagcat tagcagctgt aactttgctg ctggaagaat
    17221 tcccaaaact aaaaattcgg ttcatcaatg tcgtagattt attgaaatta cgtcatccaa
    17281 gtcaagatcc gcgtgggttg agcgatgaag aatttgatca atattttaca aaagacaaac
    17341 cgatcttatt tgctttccac ggttatgaaa cattggtccg cacgattttc tttgatcgtc
    17401 ataaccatca tttaatgatt catggctaca aagaaaatgg agatatcacg acaccatttg
    17461 acatgcgtgt agtaaatgaa cttgatcgtt atcatttggc aaaagacgca gctttaaaaa
    17521 tcaaaggaag tcaagcagaa gattttgcga aaaaaatgga tcagaaatta caagaacatc
    17581 aaaactatat tcgtgaaaat ggtattgatc tgccagaagt attggattgg aaatggaaaa
    17641 atcttgacca ataaaatgat aaaatcgatc ccgcaaaata gaatttttta cattgattaa
    17701 gaaactgaaa aaataaataa aaaaaacgga aaaaagactg gaagaatggc cagtcttttt
    17761 tttcgttttt gggaagtgaa aaccaaacta tttacctgta agccacccat aaatccaatc
    17821 aaatggtgca aagaaaaaat cttccattct attccctcct ttaaaaaagc ataacatgat
    17881 ttctatttac gtgagcatga attttgttac gtttttattg ccgaaaaata aaaaccgttc
    17941 attatgatgt caaattcgtc agtaattgat gcaatgtgtt ataataattc agttaaatca
    18001 aaaatgaaat ggagaaaaga catggacggg ttattacctt tatggaaaga acgcggaatg
    18061 acaagtcatg attgcgtatt caaattaaga aaaatcttac atacgaaaaa aatcggacat
    18121 gggggtacgc ttgacccaga cgtagacggt gtgttgccca tttgtattgg aaaagccaca
    18181 aaagttatcg aatatttgac agattctggg aaaatctatg taggcgagat aactctaggt
    18241 ttcagtacaa cgacggaaga tcgatccgga gaacgggtag aaacaaaacc agtagatatg
    18301 tctttatctg aaaaaaaaat agatgaagtg atggcttcat ttattggtga gatcacccag
    18361 ataccaccaa tgtactcagc ggtaaaagtc aacggtaagc ggttatatga atatgcccgc
    18421 aataatgaaa cagtggagcg tcctgttaga aaggctcaga tttaccgttt tatccggaca
    18481 agtgaattga caagagacga agtgacagga accatttctt ggcgcttcga agtagaatgc
    18541 gggaaaggaa cctatgtccg cacattagct gtagatacag gcaagaaact tggttaccct
    18601 gcgcacatgt ctgatctgac ccgaacagca agtgccggta tgtcccagaa ggatgctctg
    18661 acattagaag aagtagctca agctgtgaaa aatgggacaa tcgatgattg tttgctgcca
    18721 atagaaaaag gagtggaacg gttcaaacga gtagatatta ctgatgaagt atggcagaaa
    18781 gtaaaaaacg gtatgagact tgattatact gtatttgcgc tcccagaaat gccgtcagaa
    18841 gaaattgctt tgttttataa aggaaaagtt gtcagtattt atcagccaaa cccgaaagaa
    18901 aaaaacaaat taaaacctag taaagtattg agaaatgagg aataacgaat gaatatcatc
    18961 aagatcagac atccttacca atcagaacaa attccagaag acgatgtcgt tttagtgctg
    19021 ggatttttcg acggcgtgca tatcggacac cagaaagtca taaagacagg tatggaaatt
    19081 gcacaaaaag aaggattgaa attagcattg atgactttca atcagcatcc atctattgtc
    19141 tttaaaaaga tcgatcctag cagcgtgaaa tatttgacta gccttaagca aaaagaagaa
    19201 aaaatggccg ctttgggtat agattattta tatgaagttg attttacttc ttcttttgct
    19261 catttagcac cgcaggaatt tgttgatcaa tacatcgtag ggttacatgc aaaatatgct
    19321 gtttccggtt ttgattatac gtatggacca aaagacattg cagatgtcgc tcattttcct
    19381 gaatatgcca aaggaagatt caaaatcgtc acagtttcta aagaagaaga acaaggtgaa
    19441 aaaatcagct ctacccgtat tcgtggatta ctagacagcg gacaagtaaa agaagcagca
    19501 aaactattag gttgcacata tgaggtagaa ggggtagttg tccatggcga tgcacgaggc
    19561 agactactag gattccctac agcaaatgtc aaagtaaaga gcacggttca tttgcctaaa
    19621 gaaggtgttt atgtgtcgga aataaaaatt ggggaaacat ggtatccagc catgggttca
    19681 attggacata atgatacatt cggtgaagga cgggagctga ctgtagagtt gtatattttg
    19741 gactttaatc aagatatcta tggtgaacat gtcgcggtac gctggaatca atttttaaga
    19801 gatcaagtga aattttctag tgtagaggaa ttgattgaac aactcaagga ggatgaacaa
    19861 gcaacagctg aatatttttc tcagcaataa aacattatgc ataaaatctc ttgtttatgt
    19921 ataactagca attacaataa aatgggtgat aaataagccc agg
//
LOCUS       DACCDF010000048        19751 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCDF010000048 DACCDF010000000
VERSION     DACCDF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.051"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..333)
                     /locus_tag="IVK51_001754"
     CDS             complement(<1..333)
                     /locus_tag="IVK51_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431337.1"
                     /translation="MTDIVKVKQNDVQVYPQTHWDAVEGKPETIKGDKGDPGQAATIT
                     VGTVTSGTTASVTNAGTASAAKFNFVLPKGDKGDKGDPGANATTTAVATTTANGLMSK
                     EDKVKLDGL"
     gene            complement(338..1126)
                     /locus_tag="IVK51_001755"
     CDS             complement(338..1126)
                     /locus_tag="IVK51_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431338.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKVGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATV
                     DRLGGIKIGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGKI
                     NADVTGFYVLPTASEFVKGGIRVGEGLTMTDDVLSADKQFNYTAGANISISNTGVISA
                     TGGGEGGGVTQEYVDQKMSEAYQNAQAYTDSKIPNVSFEKVGEV"
     gene            complement(1126..2034)
                     /locus_tag="IVK51_001756"
     CDS             complement(1126..2034)
                     /locus_tag="IVK51_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6431339.1"
                     /translation="MAKWNVVLSTTEPYNYVGMIQVRQGNKNSEVMEATITENGMPYD
                     LTGCKVYFESVVGDKYPVQLGTKVIDAKKGKIQYTFDQYSMQCLHRQTADFIIYKDDE
                     LIATTQDFSYFVIKAVSKTEGEMGSYWQTVEDLIADMSAFINENKGDFTDWMNARKKE
                     FEQWRQDQQNTFEAWREGQETDYLKWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGTLIIATVDDPKQNVFTLEKVGVI"
     gene            complement(2000..2518)
                     /locus_tag="IVK51_001757"
     CDS             complement(2000..2518)
                     /locus_tag="IVK51_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6431340.1"
                     /translation="MIEEKGLNHLKSLLNQPIGNHQCYALSAEYAGVMIGPDMGAGTK
                     YEIKVRHGNVFSAAEIGRAYPWALYLWTVIVHPEYDQLVVGSIINWERNAKISDTFES
                     HEYYGHTGVIKGLENGRIQTYEQNAESGGIVAEYDREFFGSGQIASICIPPDFEKGVV
                     INGKVERSTQHN"
     gene            complement(2529..5351)
                     /locus_tag="IVK51_001758"
     CDS             complement(2529..5351)
                     /locus_tag="IVK51_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6431341.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRGGQYRSYLKKGNFIKAKVPDGSWQYFEIYNIKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGEMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAMTSKGEIVADFI
                     KVGIINAEVLESSINDLGDVLKIVKGTLQIWNKNKKIMELTKKGMEFWNTKESIGTIG
                     TTDSAGNPFPGASTPTPLEDNSLVIRTNGDGKYILISPKEGKGWVILGNGTSILFGSL
                     NLQEKLNAFGDAEFMKNVNIRGKLTINGQEVFPGQGGSGNNDGGSWNGMYPPEVTSQA
                     DKFAWELWVMLLSRGYSKASIAGILGNVQGEAGAAMNPDIAQVGGPAYGIVQWDGSAY
                     PLVPPATWDGRTYVQNLMRTAGITEDYRTMSAQGKLLDWTMYNGQWLGIVQPASVSGF
                     KAMTDPAAAAYTFERNYERPATTHPERQGWAVNWYNKFKDLQVPSAGSILSTAKSLMG
                     YFHYSQPLRWNFGSVENPDRNGYADCSSFVWLALTKSGYKTATRGTLWYTGSMAADAR
                     GPRQYLTEISPNKAKAGDIIIVNQGAGAGNDGHTAILAEDWKGYSTSIVEMGGMNSNG
                     VGIGRVDWSFGYLLNGGDVCLARAKK"
     gene            complement(5348..6052)
                     /locus_tag="IVK51_001759"
     CDS             complement(5348..6052)
                     /locus_tag="IVK51_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431342.1"
                     /translation="MNPDEPNFIWKKRNAVIDMDCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLDAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKV
                     SGKFKEVRFWNRSVYL"
     gene            complement(6064..8373)
                     /locus_tag="IVK51_001760"
     CDS             complement(6064..8373)
                     /locus_tag="IVK51_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP6431343.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLGELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGGVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRRGIGGKVSGGNIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANQQAIYDLGGVPI"
     gene            complement(8568..9032)
                     /locus_tag="IVK51_001761"
     CDS             complement(8568..9032)
                     /locus_tag="IVK51_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431344.1"
                     /translation="MARLSDYGINVEDLKNSATVTIQGVEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKTQAKRKVNKKNRKR"
     gene            complement(9032..9658)
                     /locus_tag="IVK51_001762"
     CDS             complement(9032..9658)
                     /locus_tag="IVK51_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431345.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVVRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHEIALDHVGIPIAVMDEIKGMIAKSGVTFSKN
                     TAREFPYLAFGFIGNIEGGGKKAVWYPNTQLSNVIDEEYATAEDETKIDDVTANFVST
                     GLKYNNVMYASFDSNREEATMDLFGKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(9665..10030)
                     /locus_tag="IVK51_001763"
     CDS             complement(9665..10030)
                     /locus_tag="IVK51_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6431346.1"
                     /translation="MPSNKNGFSEISDYLGNLSRVDPKKLSLESLEEAAKFYLEQLLP
                     NIPKSLLKKKHMSEQIKVVVEEDRVKVQFEETAFYWRFTENGTTKRKAQHFASGTYEQ
                     NKEKIEEIMTKKILDLWEG"
     gene            complement(10020..10358)
                     /locus_tag="IVK51_001764"
     CDS             complement(10020..10358)
                     /locus_tag="IVK51_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431347.1"
                     /translation="MSISFDELKTALKSTRLPVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(10348..10674)
                     /locus_tag="IVK51_001765"
     CDS             complement(10348..10674)
                     /locus_tag="IVK51_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP6431348.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(10655..10933)
                     /locus_tag="IVK51_001766"
     CDS             complement(10655..10933)
                     /locus_tag="IVK51_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP6431349.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(10935..12308)
                     /locus_tag="IVK51_001767"
     CDS             complement(10935..12308)
                     /locus_tag="IVK51_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP6431350.1"
                     /translation="MDTELLKKMKARREQRLTELREKVESGELREADLEAITKEIDAV
                     VDELNGIKSELSEADGSDEGSDADEGTGGSDGSTGSDENRSGEDNDQEEDSDSEDSSE
                     NRSGMITQQQRDGLLGSIKNGLEARAKMTNKQKDQQLRKAFANFVVGNISEAEARALG
                     IEAGNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKE
                     RSTDIAETAIQFDEILLDPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEIN
                     YMFNGDDAGNENPGALAKKAVAFEKPLDLTAAGAGQKLYDALIEFKNTPVTEVMKKGR
                     FIINRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGKPDTPV
                     LYFGDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYE
                     ITKPVGG"
     gene            complement(12321..12896)
                     /locus_tag="IVK51_001768"
     CDS             complement(12321..12896)
                     /locus_tag="IVK51_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAP6431351.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSPTLLWD
                     DLSEVIAPGAFARTISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTISEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(12862..14049)
                     /locus_tag="IVK51_001769"
     CDS             complement(12862..14049)
                     /locus_tag="IVK51_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6431352.1"
                     /translation="MEKRGYIEDMFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALSSPVVIGPDGNEVKDHHLLKILKNPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQDDSNHSVKM
                     IPLGKGYTIETLKSPVDDQAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQNLKGGDGNEEEGN
                     SHD"
     gene            complement(14113..15840)
                     /locus_tag="IVK51_001770"
     CDS             complement(14113..15840)
                     /locus_tag="IVK51_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP6431353.1"
                     /translation="MIEPGVNYADLFAKEVRKRPKKYPKTVRLAIDRWYRWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGDMVGKPFILEPWEKFIYSWIYGWVRENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGALGYAMYGEGALSVNCYACASDFAQAQYAAQPFADSILN
                     NPVLLDGTKIFKGPKGTVSSITYEYLDGDLALKNKFIVQTKNIENIEGANPYFVCNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSS
                     FVLIYEPNKGYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGNLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MDLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(15837..16289)
                     /locus_tag="IVK51_001771"
     CDS             complement(15837..16289)
                     /locus_tag="IVK51_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAP6431354.1"
                     /translation="MTTKAQRKAIVDEKVSAEKARILKIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWSDKKMKALDLLGL
                     TNKAKTGRQITGGSTARKDEEITRPEEKPVDELAAHRNKWRKKAGTEK"
     gene            complement(16401..16781)
                     /locus_tag="IVK51_001772"
     CDS             complement(16401..16781)
                     /locus_tag="IVK51_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6431355.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIIENETEDKKVFPSYFN"
     gene            complement(16778..17164)
                     /locus_tag="IVK51_001773"
     CDS             complement(16778..17164)
                     /locus_tag="IVK51_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431356.1"
                     /translation="MAMIKIRTTITGTEYWDSEKKKTVVVPKGQEPDFEATEEKGILS
                     DGKTFVAVNGELITNSSEILDSDGNTAADFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREAE"
     gene            complement(17145..17450)
                     /locus_tag="IVK51_001774"
     CDS             complement(17145..17450)
                     /locus_tag="IVK51_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431357.1"
                     /translation="MNDFHEAMLMANVPTNMGDVYKKAIEAENSPTGLRNNWNGTYAN
                     VQVDKDNRGIAMDVLVISLLSHTLPNLQETVEWYERMGAKVISTNYKGENQNGNDQN"
     gene            complement(17755..17898)
                     /locus_tag="IVK51_001775"
     CDS             complement(17755..17898)
                     /locus_tag="IVK51_001775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431358.1"
                     /translation="MRIGNVYLISKVPYQKLLYSCKVRFIASDLRNLRKQFILSDADE
                     LAD"
     gene            complement(18223..18690)
                     /locus_tag="IVK51_001776"
     CDS             complement(18223..18690)
                     /locus_tag="IVK51_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP6431359.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTANHDFEAERMLIDKEEAQEEFEYLHKLFIRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(18764..19204)
                     /locus_tag="IVK51_001777"
     CDS             complement(18764..19204)
                     /locus_tag="IVK51_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6431360.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(19234..19527)
                     /locus_tag="IVK51_001778"
     CDS             complement(19234..19527)
                     /locus_tag="IVK51_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431361.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIKS"
     gene            complement(19527..>19751)
                     /locus_tag="IVK51_001779"
     CDS             complement(19527..>19751)
                     /locus_tag="IVK51_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431362.1"
                     /translation="IFEGDVVSVSVRNGFDYLDNKVCIVKNSIGHSGLVCATVDEDLE
                     YRIFNTELFEEYTYEVIGNIYENSELLEEK"
BASE COUNT         5436 a         4420 c         3190 g         6705 t
ORIGIN      
        1 taatccatcg agtttcactt tatcttcttt ggacatcaaa ccatttgctg tcgtcgtagc
       61 cacagctgtc gttgttgcat tggctccagg atcgcctttg tcccctttat caccttttgg
      121 caatacaaaa ttaaactttg ctgcacttgc agtaccagca tttgtgacac tagctgttgt
      181 accactcgtt actgtcccta cagtaattgt ggcagcctgt ccagggtctc ctttatctcc
      241 cttgatagtt tcgggtttac cctcaacagc atcccaatgg gtttgagggt aaacctgaac
      301 atcattctgt tttactttta caatatctgt catttgtcta tacctcccct actttttcaa
      361 acgatacatt tggtattttg gaatctgtat aagcttgtgc attttgataa gcttcactca
      421 ttttttgatc aacatattct tgagtgacgc cgccaccttc tccaccacct gttgcagaga
      481 ttactcctgt gtttgaaata ctgatattag ctccggcagt atagttgaat tgcttatcgg
      541 cagacaacac atcatcggtc atcgttaatc cttcaccgac acgaatacca ccttttacaa
      601 attcggaggc agtgggtaaa acgtaaaaac cagtcacatc tgcatttatt tttccatctt
      661 catcaatagt gatgttttct ccagcagtat aatttttcag tgaatcgagt ttttctttta
      721 actccagcga aaaattgaca tcagattgtc tgactgcaga gactttccct gtctcatcaa
      781 tcgtcaataa ttcaccaatt tttatgccac ccaaacggtc gacagtggca actggcaaca
      841 cgtagtcacc ttctccactg tttaaaattt tttgatacat ttctgccgtg ataatgccgt
      901 catcttcttc acttgcatag ggtaattctg tgagaacatt ctctaatccc aaatcttctt
      961 tagtaatgac gacggctcca acttttccat taacagataa aactgttgct tctcctgcta
     1021 ttatttcttc taacccaaga actgcggaag catgtgtgat tggaaagaac tggcgttgta
     1081 cacctgtttt ttcatccgtt tccatcattc gtttagtttt agccattaga tcaccccaac
     1141 tttctcaaga gtgaatacat tttgctttgg atcatcaacg gttgcgatga tcaaggttcc
     1201 ttcttcaatc gggaattcta cagttccaac tttttcgact tcgtgattat ctgaaaagag
     1261 atcatcctgc aaaatatcag tcacttcaat ctcgccgtat tcaatcgtga aaagtgttgc
     1321 tcgtaatttc tgatatagat aatccatatc tgccagcaag cgctctgaaa tagacgcatg
     1381 acgaactcct tgaatatcaa cacgtgcatc cattaattcg gctaacatta ctccacctgg
     1441 atcgatagac ttcaaaatat ctttgattga ttcaaaccat tttaaataat cggtttcttg
     1501 tccttctctc catgcctcaa atgtattttg ttggtcttgt cgccattgtt caaattcctt
     1561 tttacgtgca ttcatccaat ctgtgaagtc acctttgttt tcattgataa aagctgacat
     1621 atccgcaatt aaatcttcga ctgtctgcca atacgatccc atttcaccct ctgtttttga
     1681 gacggcttta atcacaaaat aggagaagtc ttgcgtcgta gcaattaact catcatcttt
     1741 ataaatgata aaatctgctg tttggcgatg taggcattgc atcgaatatt gatcaaatgt
     1801 gtactgaatt tttccttttt ttgcatcaat gactttagta cctaattgaa ctgggtattt
     1861 gtctccgaca actgattcaa aatagacttt acaaccagtt agatcatagg gcattccatt
     1921 ttcagtgatg gttgcttcca taacttcgga attcttattg ccttgccgta cctgaatcat
     1981 accgacatag ttataaggtt cagttgtgct gagtactacg ttccactttg ccattaatta
     2041 ccactccttt ctcaaaatca ggcgggatac aaatagaggc tatctgacca gatccgaaga
     2101 attcacggtc atattccgcc acaattccac ctgattctgc attttgttca taggtttgaa
     2161 tacgcccatt ttccagacct ttgattacac ctgtgtggcc gtaatattca tggctttcaa
     2221 atgtatcact gatttttgcg tttctttccc aattgataat tgagccaaca actagttggt
     2281 catactcggg atgaacaata accgtccata aatacaatgc ccatggatag gctcgtccaa
     2341 tttcagcagc ggaaaataca ttgccatgac gtactttaat ctcatattta gtaccagccc
     2401 ccatgtcagg tccaatcatc acaccggcat attccgcaga taatgcataa cattgatgat
     2461 ttccgatagg ttgattcaac aaacttttca aatgatttaa tcctttttct tcgatcacaa
     2521 aatcacctct atttcttcgc tctggcgaga caaacatcgc caccgtttaa taaatatccg
     2581 aaggaccaat cgacgcgacc gatacctaca ccattggaat tcataccgcc catttcaacg
     2641 atagacgtgc tatatccctt ccaatcctct gctaaaatag cagtgtgtcc atcattacca
     2701 gcaccggctc cttggttgac aataatgata tctccggctt tcgctttatt tggagatatt
     2761 tcagtcagat actgacgtgg ccctcttgca tccgcagcca ttgagcctgt ataccacaga
     2821 gttccacgtg ttgcggtttt atatcccgat tttgtcaaag ctaaccaaac aaaagaagaa
     2881 cagtcagcat acccattacg atcaggattc tcgacgctac caaaattcca acgtaacggt
     2941 tgcgagtaat ggaaataacc catcaagctt ttggctgtac tgagaatact gccagcagat
     3001 gggacttgaa gatctttgaa tttgttatac caattaactg cccatccttg tctttctgga
     3061 tgagttgtgg ctggtctttc ataatttcgt tcaaaagtat aagcagccgc tgctggatca
     3121 gtcattgctt taaatccaga aacacttgct ggttgaacaa ttcctaacca ttgaccgtta
     3181 tacatcgtcc aatctaataa cttcccttga gctgacattg ttcgataatc ttctgtaatt
     3241 cctgcggttc tcatcaggtt ttgaacataa gtcctgccat cccatgttgc tggaggaacc
     3301 aaaggatatg ccgaaccatc ccattgaacg attccataag ctggaccgcc gacttgtgca
     3361 atatctggat tcattgcagc accggcttct ccttgaacat ttccaaggat tccagcgatg
     3421 gatgctttgg aataccctct agaaagaagc atgacccata attcccatgc aaatttgtct
     3481 gcttgactgg taacctctgg tgggtacatg ccattccaac taccgccatc attatttcca
     3541 cttccgcctt gtccagggaa tacttcttgt ccgttaattg tgagttttcc gcgaatattg
     3601 acgtttttca taaattccgc atcaccaaag gcatttagct tttcctgtag gtttaaactt
     3661 ccaaataata tagaagttcc attcccaagt attacccaac cttttccttc ttttggtgaa
     3721 atgagaatat attttccatc gccatttgtt cgaatgacta acgaattatc ttctaacggt
     3781 gtaggagtgg atgcgccagg aaaaggattg cctgcagaat cagtcgtacc aattgtgccg
     3841 atggattctt tggtgttcca aaactccatc ccttttttgg ttaattccat gatcttttta
     3901 tttttattcc aaatttgtaa cgtacctttg acaattttta gcacatcacc tagatcatta
     3961 attgagcttt caagaacttc tgcattaata atcccaactt taatgaaatc agctacgatt
     4021 tcacccttcg acgtcattgc gatgccaaac gggccattta ctccgttgtc tgaataacct
     4081 aagccattta aattccaacg ccacacacgc ttcgcattcg ctacattggg agtatccata
     4141 ataagaatct ctgatggagc tttttctgga cgaaaaacga catgcccacc agaattacca
     4201 gtgatccatg ccgtcgcatt caacacattt tgaactaacg tttctgttcg attgtcgatt
     4261 ttctttttca attcttgtgc ttgagtattt actgccgaag tgtacagtga taaatcatta
     4321 cctaaaacaa tatccttata tttcccaagc gttgggaaat aagtgtattc gaccatacgc
     4381 tcttttatct cgatgtctag ctctttcgcc ctaacatgag cgacatcacc aaagtgtaaa
     4441 gacgctagct cttgatacat ctcaccgtat tcgagtgtgt gttccaacgc caccatgctg
     4501 actgtatgag tagcttttgg ctcgtgaata cggtcattgt caaataaggt tttgccccat
     4561 ttgatcaatt catcaactgt tttacaatca ccattttcac gtttggcaat acgtcgattc
     4621 tcattggtca ctccatcaat ttctaaataa ccatattcga taggttcttt gtcttcatca
     4681 taatcgttat caggaacccc gccaaccaaa tacaaactat tgacaattga ttcttcatca
     4741 atttcttctt cgattgcttc taaattgatg ccaaaatcga tccgaaaacc attatctgat
     4801 ccaatctgtt ttaccagttt caaatcatag ttatccatat caagttccgc gctagtcacg
     4861 ccagtcaaat tttgattacc gttatttgaa ccgataatcg cttcaactgg tgcgacttgt
     4921 cttgctgtaa attggtgcgt tgtacctaca ttcgacagat agttgaattt ttgatcaaat
     4981 gccaaactgt ttttaagatt ggtcatgatt tgagaaccgt tgccgttatc cgtgaatgac
     5041 ttaacgataa agtttttatt ggccataaaa ccaatatgtc tggctgtcac agaaacggat
     5101 gtcaaattct ttttgatatt gtaaatttcg aagtattgcc atgatccatc tggtaccttc
     5161 gcttttatga aatttccttt ttttaagtaa gaccgatact gaccgcctcg tgaatagtta
     5221 ccataaaaac gatattgacc atttaataca cgattgattt ctggcaaatc ttcccagtca
     5281 accaacgacg ctccgttaac actcaaatca tctggcattt ttttgtaagc ataaataaat
     5341 tcttgtgtca cagatacaca ctcctattcc aaaaccgcac ttctttaaat tttccggata
     5401 cttttacctg attccattca ggctgcaata ctggccaatc accacgcgta aataaattca
     5461 aaccttcttg tattgctttc ccaagctgag tatcaaccac gattgttgca gctaaagtat
     5521 taagtattgt taggcttttg tcaccgacag cgatcgtaat atctcctccg ttcgattcta
     5581 tttccagata aggatgtgca atttcgtcac catgatcaaa aatatccata acactagaat
     5641 gaaaaatttt aggtgcttca ccgatttttc ttttgagtgg ctgacaacga aaagtaacat
     5701 cgaatgtata aaagaagccc cactcgtttt cgaatgggac ttctttatcc atgctgcaga
     5761 tagcatctag atacttgtct ggatcattat gagtgattaa ttggctttta ccagtaagcc
     5821 atcgtttgac ttccctcagt ttcgaatgtg ggatagtgat cccttctatt tccaaatcaa
     5881 aaggttcata atcatcaaac gtctccgtca attctccgct tcggccctga atcgtgtaag
     5941 tttcgtatcg cttgttcggc ataatatctg gaagctctgc ctcaatgatg caatccatat
     6001 caattactgc attccgcttt ttccaaataa aatttggttc atctggattc ataaattgtc
     6061 gactcaaata ggaacacctc ccaaatcata aatagcctgt tggtttgctt tagcaaattt
     6121 acgattcata cgatctaact cagatggatt gtttgcatcg actttgccaa tgtggatatg
     6181 ctgttcaata ttaccaccag aaacttttcc gccaatacct ctgcgttttt cttcgtcaga
     6241 taatggggta actgttgttt ttccattttt tgcagtcagt aactcaggtc cagcttcacc
     6301 aacaatcgct tgtccattga ttagatgacc accttgtgct aaataaggca gttttgaaat
     6361 agaaaatgat tttccaccta caccaggaac ccattttggt atctttatat tattcaatcc
     6421 accaataaat ccattgatca atccaatcat ggcattaata ggagctttcc ctacagcaac
     6481 aataccatcg aaaatacctc caaaaatatc aactagacct tgccatgctt ttgaccaatt
     6541 ccctgtaaat actccagtga taaaatctag aaaaccactg aaaattcgtt ttccagcatt
     6601 ataaaaatta ttgaaatttg ctataacacc atcgaaaaat ccaccaaaat aaccacttaa
     6661 aaaattaaat atttcaacag ctacatctga aacacctttg aagaaagaat tcactccatc
     6721 atgaaaccat ttaacgttat tatatgccca aattagtcca gtaactaatg cgccaatagc
     6781 tataactgct aatgcaaacc agcctcccga cattccaaat aaaccagcaa gtccttgcca
     6841 aactcctata aaacttttaa ctcccccaac gatttttgta acagatccca taagtgaacc
     6901 aagaacgatt agtactggtc caactgctgc cgctatacca gcaattgtta tgatccaatt
     6961 ctttgtatct tgatctagag acccccacca tttagagaat tcctgtaata aggaagttgc
     7021 tttttcaaaa aaaggcaaaa ggcttacttg gactgcttca cctacgtcag ccattgctaa
     7081 ttttgcattg ttcatagctt gatcggcttg atcaattggg tcgagagtag catcgaatgt
     7141 gtctcctaca gctcccccac tttctccagc agtttttgct aaatcttcca ggttcaaagt
     7201 acctctacga attgcatcgg ccattctagg accgccttta gtcccgaata cttctgctgc
     7261 tgcattgatg gcttctgttt cagaacctgc attttttacc ttgtcttgca attccccgag
     7321 accctgactt agagattttc catcttttgc ataagctact gtcgctttag ataaactact
     7381 caaagctgca cttgagtcaa caccgctttg ctcaaattgt ccaagtaaag tgaccccttc
     7441 accaaagctc aaccctaatt gtttaatttg tggtgcgcca tcaattgctt tttgcatcaa
     7501 gtcgtctaca gattgaccag tattctgcga tgttttcgtt gttacatcaa ggacagaatt
     7561 caaatcatca tattctagtc cataagcatc aatagcttgt cgagcagata ttgctgattg
     7621 cgaaacatct gtatcattga tttcagcgta ctgtaggaga taattggttg aatcttccag
     7681 ttttttatcc atgaatccaa attgtgtatt tacctcacca atagcttccc caaccgtttg
     7741 taaaggtaaa tgagtatttg aaccaacgtt tttgaaagac tgtgaaagtc tatcagcttg
     7801 atcacctgtt gctccggttt ttgtaatgat ggtatcaagt gcctcgtcaa cttcaccaaa
     7861 tgctgcaagt cctgctgctc ctgccgctac aataggcgcg gttactccaa cagacatttt
     7921 ttcaccaacg ccttttactt tctcacctgt ttcttcgatt ttctggagtt tttttgctgt
     7981 atcaacagaa acgtcacctt gttctttaag tgcatcgttc gtctgttcta atgctgtacg
     8041 aagtttattt tctccggttt cagaattgag taattgttta taaagttttt ctgattcagc
     8101 agaatattca ccggtttctt tgacagattt ttcatattcc tctctcagca tttgagatcg
     8161 ttgttcagct aaccccagtt gtttttctaa cttttgttta gccgctcgca atttttctgt
     8221 ttgagttgca tccttgtcca tcgcagacac ttggtttttg tactcggcag cggctaagtt
     8281 catttctttg ttgatttctt tgattgtttt cgaataactg acttcgccat tcgttttaaa
     8341 attaagaacg acatcagatt ctttaccagc catttatctt agcgctcctt tctaccacca
     8401 aggcgattta tccatagtta cagattgagg tggttcaaaa tcggtgtttt gttgtaacca
     8461 ctgtaaataa gatttgagcc acaagttagg tgtagacttc aaaaagaaac tctcactcca
     8521 attcaataga gtgagagcaa cgtaaatata aaaactccaa ggagttccta tctcttccga
     8581 ttctttttgt ttacctttct ttttgcttgc gttttttgaa agtcttgtgg cttcttggat
     8641 ttttttaggt cttccacctg aaatgtctgg ctagcaaaaa tttccataca ggtactataa
     8701 gcagacaaca cctctccact cattcccaaa aatttgaaaa tagtttctgg atcttcctct
     8761 aaaccaccag tacgcagcat ggcataaatc aaggcacgca tgatttttag atcactaggc
     8821 gataagttag cagaagaaat ttgtccttct tttttagata gcatggcgtt catatcagat
     8881 tcaaattgcg aataatctcc accatacaca tctgctataa attccattgt ttgcatagta
     8941 aatgagatag ggaattctac accttgaata gtgacagtag cagaattttt taagtcttca
     9001 acgttaattc cataatcaga taaccgagcc attagcctgc acctccaact ttagccaatg
     9061 ttttccactg ttcttcatcg tatacaggtt gagcaatgaa ttttccaaat agatccattg
     9121 tcgcttcttc acgattcgaa tcaaaacttg catacatcac attattgtat ttcagtcctg
     9181 tagaaacaaa gtttgcagtt acatcatcga tttttgtttc atcttcagca gtagcgtatt
     9241 cttcatcaat tacattagat agttgcgtgt ttggatacca tacagctttt ttacctccgc
     9301 cttcaatatt tccaatgaat ccaaatgcaa ggtaaggaaa ttctcgtgcg gtatttttac
     9361 taaatgttac tccgcttttt gcaatcatcc ctttgatttc atccattacc gcaattggaa
     9421 tacctacatg atccaacgca atttcatgtt ttgtttctcg ggacacgcgt cggaacattt
     9481 tgcttgatgc ccatttttcc aatgctgtac cattgccttt gactccaatt tttgtagcga
     9541 taggcaaccg gacaacttca ctataagtcg gagcagttcc aacagaatct ggcgttgcca
     9601 tcatcgcgat taaaatgtcg tccaatcctt caaaataaaa agtatcttgc tttcccaatt
     9661 aaaatcatcc ttcccataaa tcaagtattt tcttcgtcat gatctcttcg attttttctt
     9721 tattttgttc atacgtaccg cttgcaaagt gttgtgcttt tcgttttgtc gtaccatttt
     9781 cggtaaagcg ccagtaaaaa gcagtctctt caaactggac tttcactcgg tcttcttcaa
     9841 caacaacttt tatttgttcg ctcatatgct tctttttaag cagggactta ggaatattag
     9901 gaagcaactg ctctaggtaa aactttgcag cttcttctaa agattccaat gataattttt
     9961 ttggatcaac tctagaaaga ttccctaaat aatccgatat ttcagaaaaa ccattcttat
    10021 tacttggcat tttccacaca cctcacatat gtgtaatagt tggtcacggt atcgtcattc
    10081 tcatcacctt gtatacctgt aaagtctgaa tatggaatgc cagcattttg cagcgcttgt
    10141 tcaataacaa ctaaatcctg ttctgttcca agtgtaaaaa aagagacttg gtaataaggt
    10201 aattttttat agactttacc ggatgccatt tttttgctgt tactcacatt tgagtacaca
    10261 atgtatggat acatcgtccc taatctggct ttgtctctga acactggtaa ccttgttgat
    10321 ttcagcgctg ttttcaattc atcaaagcta atcgacataa gctaaactca actccatttc
    10381 tcttgcatcg ggattcgtat aaatgcgagt aatgttatac gttacagaat caattttgag
    10441 cgcacttaat ttctctgtga tggatttatc ccatctgact ttaattcgtc tgacaacgtc
    10501 tgtcttggct tgctgtgata aatatttttc ttgagaagtc acaccgagtt cttcatagaa
    10561 tattagacgc ttaaattcgt aaattgtagt tggacgatcg tttccgtctg ttcctgtttt
    10621 gatgtctagc aattcggctt tccatctgag attattagtc tgtctcttcg gcattttgaa
    10681 tcgctccttg cacgataaat ggcgtcatgg cattcatagc tttgtcgagt tcatcctctg
    10741 aaactctata ttcataggca atgccggcaa ccatcaaaat aagatattct tgttggccac
    10801 cagttgctgt tttgacataa tcttttgcca tatttaaata aaaagagagc aaagaatcat
    10861 ccatgccctc ttcaaaatga atatgtgatt tgaatttttc ctctaaagac aattctttag
    10921 tttgctcttc catcttaacc accaactggt tttgtaattt cgtagcgata tactgccggt
    10981 tcaaatggag aataaaccaa ttgaccatct agcaagttgt aaatttggaa tccaatttga
    11041 tttttaccag agaatttttc aacaagtttt tgaatttcca aggcaccaat aacttcttga
    11101 attttaaatg cagaaaaatc gccaaaatat aaaactggtg tgtctggttt acccttttta
    11161 tctgctgcat ctgtccaatc cacaggatag ccaactaatt ggtaacctat tccaccttct
    11221 gcttgtgtaa atggacgcaa caaaggaaat ccatcatctg ttttcatttt ttcaatagca
    11281 gtcaaagcag ctcgattaat aataaagcgt ccctttttca tcacttctgt cactggtgta
    11341 tttttaaatt cgattaatgc atcatataat ttttgcccag cacctgcagc agttagatct
    11401 aaaggttttt caaatgctac agcctttttg gctaatgcac caggattttc atttccagcg
    11461 tcatcaccat tgaacatata attgatttct ttacgcacat aagctttttt caattcttcc
    11521 acaacaatat cttcaactgg aacaccagac atttttagta attttttagt tactgttgcc
    11581 aaagcatcga attcggcagg atcaagcaaa atttcatcaa actgaatagc tgtttcagca
    11641 atatcagttg aacgctcttt cttgtttaca ttcgcatctg ctttcttcac aagaattgga
    11701 tatttgacat ctcctgatgt tcgcaccact gttccgtatt tacgaagtaa attttcttct
    11761 tgagcataag taataacttc agatgcaatt acttctggga cagtaactga gccgttgcca
    11821 gcttcaatcc ctaaagctcg agcttctgct tcagaaatat ttccaactac aaaattagca
    11881 aatgcttttc gtagttgttg atctttttgt ttattggtca tttttgcacg tgcctccaat
    11941 ccgttcttaa ttgatccaag taatccatct cgttgctgtt gagtaatcat tccagaacga
    12001 ttttctgaac tatcttcact gtctgaatct tcttcttgat cgttgtcttc accagaacga
    12061 ttttcatcgc ttccagtcga gccgtctgat ccacctgttc cttcgtcagc atctgaacct
    12121 tcatctgatc catcggcttc acttagttca cttttgattc cgttcaattc atcaacaacg
    12181 gcatcaattt cttttgtaat agcttctaaa tcagcttcgc gtaattctcc tgattcaact
    12241 ttttcacgta attcagtcaa tcgttgttca cgacgtgcct tcattttttt caataattct
    12301 gtatccatgt atttttcctc ctacgcttct agcgcttgat taattttttt gattaatttt
    12361 tttctttctt cgacgttttt ttctaattca tcacgacttc ttaatgctgc ctctgtatct
    12421 tcataagccg gcaaaggaac aatagaaacc tcatataatt ccacttcgct aattgttcga
    12481 agcattggtt cagaattgta gtcccacgtt tcttctgtcg gcacaaaacc aaagctacat
    12541 tgattgatgt ctccgcgttc catagattta actaagtccc ttgctaccgt tgtgtctggt
    12601 aaatcaactt cgaatttcaa tccacgttca tcttcttcaa gtcgaagggt cccgctttta
    12661 gtacgtccta gcacattgga ccaatcgtgg ttaaataagc aacgtacatc ggagttacta
    12721 atcgttctag caaaagcgcc tggtgcaatg acttcactca aatcgtccca taatagtgtt
    12781 gggctattga atacagcagc atatccacta atggtcctcg tatgagtttc ttcatcagaa
    12841 cgcgttgaaa ggttggtgat gtcaatcgtg cgaatttcct tcttcttcat ttccatcacc
    12901 tcctttcaag ttttgatcat tcgttggtaa ggaatcatct gttgcatttt tctggccaat
    12961 cctagacaag tcatttgaaa tatagatagc ttgtgtttct ggagtgttct gtttaggaaa
    13021 accaagcatt tctgccacat tatctggact tgtaatccct gtacgtacga tgttgtagcc
    13081 aatatttgtt tttgttgagt aaggaacaaa atccaagatg ttgattttcc attccacccg
    13141 atagccagaa tttggcataa aaaaaagagc ggtgtaatgt tcgctcttgt tcttcaatat
    13201 tggtttgatt gctttgttat gcagatacat cattgctttt tcgatgtctg ttttcatcaa
    13261 agactggtag gtatctacgt tgattcctaa aaatttccct aagtcttttt tatacacgcc
    13321 caaataattt aaaatagctt gatcatcaac tggactcttt aacgtttcaa ttgtgtatcc
    13381 tttgccaagt ggaatcattt taaccgaatg attgctatca tcctgcgtcc cttctaactg
    13441 atcaagtatt gctttgacaa ttttcgtttg agcgctatta tttggattga tgtgggcgtc
    13501 cagtttaagc atgaacgcga gtaaaccacc tttagtatat ttgtccgtca aaactttttc
    13561 ggcgctcaga acgccttcca gagtgtttct tgcaagatca attattccag cacctttcaa
    13621 tgaatcagtt ccgatgtttt taatgtgtcg aatcatttgg ccaggtattt tttgaccgtt
    13681 catttcaaat tcttcttgaa gtcgttcatt gattttagtg gttacaccgt atgccaaatg
    13741 aagctggtcc cgatctgtta atgggaatgt ctcgccattg atcagtaggg tatttgtttc
    13801 caatttggta aattcgaatc cggtcaaata atcattggga ttcttcaaaa tttttagcaa
    13861 gtggtggtcc ttcacttcat taccgtctgg acctatgaca acaggtgagg acaacgctac
    13921 ctggtttgag atatcctgga ccaattcata aacatcagaa gattccatga tagaggaatc
    13981 tgttacatat ctttgaccgt aacgcgtata gtggccaaac atatcctcga tgtacccacg
    14041 cttttccata aaggaataga ctgcattcga taaccgatca cgtaatttca atatttctca
    14101 ccgcctttct atttatctat agatggaact aaggtaatca tctaattcat ccgaatcaat
    14161 atctgtcatc tgattcatcg tttccttatg accacacaaa aacgccacga aaccatcaat
    14221 ctttttcttt gattgacgtt tacttggcgc tttttgtccg ttgatgttag tgattgctac
    14281 aacgttcaag gtgcaataaa ggaacaatgg attatcaaat tgaattcgtt tctcataaaa
    14341 caaccgttcg acatcatcaa aaggagcgtt caacacttta ggatattggg caacctcaac
    14401 gcattccaat cctaagttct ccaatttctc aacaagtttg tcgctcatcg ctggatcata
    14461 attcacttgt tgtatatcgt ataaatccat gcagtcttcg atgtactgca agatttgatc
    14521 ttgatcaatc attttaccat cgcaaaattc aacaaaacct tgttcagcta aatcgctgta
    14581 aggcacgtta tcttcttttt ctcgaaactc taaatcttca ttgggaataa aataaagctg
    14641 cttcacttta aggaccgctt ttccatcttc atcccacgtt gggaagttta aagacacaca
    14701 ggtcaaatct cgtgtgcgtg ataaatccaa accaatgtaa caaggttcac cacttaaatt
    14761 tccaagctct tgggtagtaa ccaaacacgg ctctacttga tcctgttcaa agaaattatc
    14821 cgcaccgttt acaaatacat ccaagtgctt cgttagaaac tcggcttttg aatgtgctga
    14881 acgttgtgcc gttttaaacg ctgactccaa ggcggaaaga tcaactgata tcccccagtt
    14941 aggattgcac atttcccaaa cttttctatc agtccaatca tatcctttat ttggctcata
    15001 gattaaaaca aacgatgagt cattgtcatc acgttttaac acttctttcg cttcacgata
    15061 aacacgcatg ccaaccgagc tgctaccttt acccgctgtt gaaatattaa acattaatgg
    15121 ttgtggtaat gatatctgtg cagacttaaa attatcgtat tgttccattt tttcctgttt
    15181 gtgcaactca tcattacaga caaaatatgg attcgccccc tcaatatttt caatattttt
    15241 cgtttgaaca ataaatttgt ttttcagtgc taaatctcca tctaagtatt cataagtaat
    15301 acttgataca gtaccttttg ggcctttaaa tattttggtg ccatcaagca aaacaggatt
    15361 atttaaaatg gagtcagcaa acggttgtgc agcatattgc gcctgagcaa aatcagaagc
    15421 acatgcataa cagttaacag ataaagcacc ttcaccatac atcgcatatc ctaaagcacc
    15481 cactgcaata agtgttttac cattcttttt gggaatttgg acatatgcct cacgagttac
    15541 acggacaatc tgaccttttt cgttttctcg tacccaacca taaatccatg aataaataaa
    15601 cttttcccaa ggctccagaa taaacggttt tccaaccatg tcaccttttg tatgaacgat
    15661 aaaagattca acccaatcca tcatttcatt tgcacgatca acatcaaacc agatatcttt
    15721 acgctttttc caacgatacc agcggtcaat tgctaatcga actgtcttag gatacttctt
    15781 aggccgtttt cttacttcct tagcaaataa atcagcataa tttacaccag gttcgatcat
    15841 ttttcagtac ctgccttctt acgccatttg tttctgtgcg ctgctagttc atctactggc
    15901 ttttcttctg gacgtgtaat ttcttcatct tttcttgcag tcgatccgcc agttatttgt
    15961 ctaccagttt tagccttatt cgttagcccc aacaaatcta gagctttcat tttcttatct
    16021 gaccaagttt ctacttgctg cgccaatgga tgcttcgatt gattagtggc acctgattta
    16081 ttcgtgaatt tttgcgtctc cggaaagcct ttttccttcc acaaaagata tttgtgttgg
    16141 taaatttcaa aaatatccaa atatgattcg attaatggat caagagtgat ggtgtacaaa
    16201 tcagacaaat tcattatttt taaaatacga gctttttcag cacttacttt ttcatcaaca
    16261 atcgctttac gttgcgcttt agtcgtcata cttgtataca cccccctttg ttttttgaaa
    16321 aatttgacct aacgatgcgc gtgactcccc gctaccctat ctccccacgc gaaaaaattt
    16381 tgaaaatgga taggggggct tcaattaaaa tacgaaggga aaactttttt gtcttccgtt
    16441 tcattttcaa taattggatg acattttgaa cataaaagca tgagattgtt tggatcaagc
    16501 ttaagcagtt cattgtcttt gatcggtaca atgtgatgga cgtgtgccct cttgccaaag
    16561 atgaactgac cgcatcgctg acagtgacca ccttctcttt cataaataaa ttgacgcata
    16621 tctttccatg cctgtgttcg atagaatggt ttgttctcat gatgataaac agactttgct
    16681 tgctgcttct tcttgcgtga tctgcttgat ctcttgtgtt cagtacagta gatacccttt
    16741 gctattttgt tcgtgcatcc gtcaaactga cagtatttca ttctgcttca cgaataagat
    16801 ttatgatatc gccttttgca cggatagcac caggaatatc aataccatgt ttcttagcat
    16861 atgcacgcaa ttcttttgca gtcatgttgt ccagttcatc cgtatcttca gtggactgat
    16921 cattagtagc ttcgtctcca tcaaaatcag cagcagtgtt tccatcgcta tcaagaattt
    16981 cactgctatt agtaataagt tcaccattaa cagccacaaa cgttttacca tcacttaata
    17041 ttcctttttc ttccgttgct tcaaaatcag gttcttgacc tttcggaact acgacagtct
    17101 ttttcttttc tgaatcccaa tactctgttc ctgtgatggt tgttctaatt ttgatcattg
    17161 ccattttgat tctctccttt gtaatttgtg ctaattactt ttgcacccat tcgttcatac
    17221 cattcaacag tttcctgcaa attgggtaaa gtgtgggata ataaagaaat aaccaacaca
    17281 tccattgcta tcccacgatt atctttatct acttgcacat ttgcataggt gccgttccaa
    17341 ttgtttcgta aaccagtagg actgttctct gcttctattg ctttcttata gacatctccc
    17401 atattcgttg ggacattcgc cataagcatt gcctcatgaa aatcattcat agataaaact
    17461 cctttcaaaa taaaaagacc actcaaagag tgatctaata tgtaatagca acctacacac
    17521 agacaagtct aatacttcct gcgcctcacc actgccacat ccctcggttg ctaacgaata
    17581 ctgaaacaga ataccgtctc tgttcatgac caacgcgaca gtacaaagct ttgtcgcaag
    17641 caggttatcg tattcctaat aaccccaact aatgtttctc tcctaaccta cacctgagag
    17701 tggcgcacgt ctgcgctagt attttatgcc ttctaactgc tagtagcatc ccgactagtc
    17761 agccaattca tcggcatccg ataagatgaa ttgtttccgt aggttcctta agtcactggc
    17821 aatgaatcga accttgcatg agtacaataa tttttgataa gggacttttg atatcagata
    17881 tacgtttccg atacgcatgt actctctccc tcaatgcgtc taccctttcc gccacaatga
    17941 cataatgaca atagacagca acggatgata gataataaga acaatttaga aggagttgga
    18001 attcacatcc ttattcttaa tatttccgtc gctatctatc gaagcttaat tgtgaaacaa
    18061 taataaaacg atgttccttt tattattatt ttgtctcaga cctatcacta atctttcgac
    18121 actatcataa tacaacattg aataggtaag tgattggtat aaaaaaggta taaaatggaa
    18181 accaaattgg taataaaagg gtataaaaag tgtaaaaact ggctacttga aagcaaccag
    18241 ttctaacgat gaagcaaatt ggatgataat tctgtttgat tctactttaa ccgattcttc
    18301 gctagtatta ttcctttgag cagttacata aatgggcaga ccattgatat aacgatcata
    18361 gaatatcttc ttgcgccttt cagtcacatc gggtttgtgc ggatgctgta tagcagagta
    18421 tcctctaata aacaatttat gcaaatactc aaattcttct tgtgcttctt ctttatcaat
    18481 caacattctt tcggcttcaa aatcatgatt agctgtagat ggtggaacca aggagtatga
    18541 tgctgtcact tttggttctc gaggttgccc tactctacat ctagctgata gatatgctga
    18601 aagaaacaca gcgacattat gttttgtgcg ttccatatca acatctttcg cgcttggtgt
    18661 ctcatatttc tttacatcga aaagtaccat cctctgattc ccccaattat gatataatac
    18721 ttatgtcgga aatattattc atagtcggag cgatccggct tttttatttt gtacgtgaaa
    18781 tgagttctcc tgatttatac gcctcagcaa attcaaccaa agctactgcc ttcattctct
    18841 cgatagttct atcggaatat cctaattttt ctcctatttg atacatcgac atcttattag
    18901 gaaaacaata actataataa agtacctgga aatggattct acttagccgt gttaatgctt
    18961 cgataaccga atctcgagta cttatcgctt caatgatttc ttcgttttga ctgtcaattg
    19021 tgaatttcaa atttttggta acagtcatca cggaaaagtc tattttcact ggtccgacca
    19081 aacgttctaa atttctgtat ctttttagta atcgtctagc atttttcctt gtttgcttaa
    19141 tatctatatt ctctaaaatg ttcacaattg cctcttctcc ttccagttgt ggaataacgc
    19201 tcattgttgt aggatagttg taaaaaagca aaattaagat tttataactt ccatatccac
    19261 caatctcgct acagctaaat tctctttgct tttcgctaac cacttgtcac attccatcgt
    19321 gttttcaatg cggatgattg ctgagtgatt atagagatgc tctacatatc cacgaaacgg
    19381 atagatgaac tcctctgctt cacagcgaac catgtcaccg actttgactt ttggtttctt
    19441 acgtgtttta gggttctttg tcggcatatc tagcattaaa ccgccgatac catgactact
    19501 agcgtaaaat ccgtctttta gtttcattat ttctcctcca ataactcgct attctcgtat
    19561 atatttccga tgacttcata cgtgtattct tcaaacagct ctgtgttaaa aattcgatac
    19621 tctaaatctt catcaacagt ggcacaaact aatcccgaat gtcctattga atttttgaca
    19681 atacaaactt tattatctaa gtaatcgaat ccatttcgca cgctgactga tactacatcc
    19741 ccttcaaata t
//
LOCUS       DACCDF010000049        19549 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCDF010000049 DACCDF010000000
VERSION     DACCDF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.052"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(38..1219)
                     /locus_tag="IVK51_001780"
     CDS             complement(38..1219)
                     /locus_tag="IVK51_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP6431363.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(1455..2963)
                     /locus_tag="IVK51_001781"
     CDS             complement(1455..2963)
                     /locus_tag="IVK51_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP6431364.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            3122..3313
                     /locus_tag="IVK51_001782"
     CDS             3122..3313
                     /locus_tag="IVK51_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431365.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            3516..4433
                     /locus_tag="IVK51_001783"
     CDS             3516..4433
                     /locus_tag="IVK51_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP6431366.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            4426..5499
                     /locus_tag="IVK51_001784"
     CDS             4426..5499
                     /locus_tag="IVK51_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431367.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            5647..6165
                     /locus_tag="IVK51_001785"
     CDS             5647..6165
                     /locus_tag="IVK51_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431368.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            6146..6538
                     /locus_tag="IVK51_001786"
     CDS             6146..6538
                     /locus_tag="IVK51_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP6431369.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            6614..7549
                     /locus_tag="IVK51_001787"
     CDS             6614..7549
                     /locus_tag="IVK51_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP6431370.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            7682..8461
                     /locus_tag="IVK51_001788"
     CDS             7682..8461
                     /locus_tag="IVK51_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP6431371.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            8454..8771
                     /locus_tag="IVK51_001789"
     CDS             8454..8771
                     /locus_tag="IVK51_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6431372.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            9015..10625
                     /locus_tag="IVK51_001790"
     CDS             9015..10625
                     /locus_tag="IVK51_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP6431373.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            10782..11630
                     /locus_tag="IVK51_001791"
     CDS             10782..11630
                     /locus_tag="IVK51_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6431374.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            11861..12340
                     /locus_tag="IVK51_001792"
     CDS             11861..12340
                     /locus_tag="IVK51_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431375.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            12373..14601
                     /locus_tag="IVK51_001793"
     CDS             12373..14601
                     /locus_tag="IVK51_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6431376.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            14730..16373
                     /locus_tag="IVK51_001794"
     CDS             14730..16373
                     /locus_tag="IVK51_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP6431377.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(16446..16991)
                     /locus_tag="IVK51_001795"
     CDS             complement(16446..16991)
                     /locus_tag="IVK51_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP6431378.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(17014..17499)
                     /locus_tag="IVK51_001796"
     CDS             complement(17014..17499)
                     /locus_tag="IVK51_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6431379.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(17489..18301)
                     /locus_tag="IVK51_001797"
     CDS             complement(17489..18301)
                     /locus_tag="IVK51_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP6431380.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(18440..>19549)
                     /locus_tag="IVK51_001798"
     CDS             complement(18440..>19549)
                     /locus_tag="IVK51_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP6431381.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         6401 a         3577 c         3856 g         5715 t
ORIGIN      
        1 tccaaagctt ttaaaagtaa ctttgctttt ttgtatatta taccagcaag ccagtcacaa
       61 tagcacttaa aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga
      121 caagatttcc tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag
      181 agaagttggc aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat
      241 ggaaattccc atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg
      301 ccatgatact accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag
      361 gggcaaagat gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag
      421 cattgatcat ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca
      481 catggaaacc atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt
      541 cttcaatgat cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca
      601 gaatataacc gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca
      661 gtgccatata agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg
      721 tgaacaaacg tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg
      781 attgtcctaa aatagcagaa gctactccat tataagattc caatttgccc atcccgttga
      841 ttttacttaa tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta
      901 gaataccgat aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg
      961 cacttccttt atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa
     1021 gaagatgatc aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta
     1081 ataatacgaa accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga
     1141 ttgctttttt gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa
     1201 tacctaataa ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt
     1261 ttgcaaataa aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat
     1321 tcattttcca ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta
     1381 gtaaagtcta taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta
     1441 taagtaaact ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta
     1501 atatcgattc ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag
     1561 tgggcttgtg ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc
     1621 gttactgcac tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag
     1681 gaatgaagtg tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa
     1741 ggaatgtcat ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac
     1801 gcatccatca atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca
     1861 atcacagcag aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg
     1921 gcagggacta ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg
     1981 attcgattaa ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa
     2041 taacgacgga tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca
     2101 aatcctacac gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta
     2161 attttttcaa taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca
     2221 gctactttat tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg
     2281 actgtggctg cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg
     2341 aaagtttcga acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg
     2401 ttcaaggaag tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt
     2461 ttagttgttt caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg
     2521 ttgactggat agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt
     2581 ttattgataa agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg
     2641 attaataagc cataaccacg taattcatcg attaaccgca agatatccat atcataagta
     2701 atcccataat caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt
     2761 atttcagctt gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct
     2821 ggaagtacac gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat
     2881 ttgtcatagt ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct
     2941 gaatcaaaac caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt
     3001 tttttgagga ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt
     3061 aaaccaactt gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta
     3121 catgaaaaaa agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac
     3181 cggttatgaa agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa
     3241 cgaagaaacg cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct
     3301 gcctagtaaa taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga
     3361 aagactagtt gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc
     3421 attgcccgct cttttttata acaatttttt acagaaaatg gtactattga agtattaata
     3481 agtgaaaaac agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa
     3541 attatgagcc agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt
     3601 cttcttttta cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact
     3661 tacaggcttt tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca
     3721 atggccgatt gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat
     3781 tgctgatgcg taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag
     3841 aaaacatctc gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca
     3901 cggtcggtat tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt
     3961 gggagaaata ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg
     4021 caaaggtgat cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca
     4081 atcgacaaat gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg
     4141 aagaaattca agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc
     4201 ttccttatat tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag
     4261 caagagcaga ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct
     4321 atttcggtag caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg
     4381 gcaatgagaa tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat
     4441 gaagcagaat ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca
     4501 ttggaagaac aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa
     4561 acaaaaatac acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc
     4621 catgcttacg aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa
     4681 gatatcgtca gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa
     4741 gatttgatcg aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat
     4801 gaagaaccaa tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta
     4861 ctgaaaccgt tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa
     4921 acaagacatt tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt
     4981 gtattaccga ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca
     5041 ccagagacaa tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg
     5101 tcagtggatc gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa
     5161 ttattagccc atgcaagaac acttggtttt accgttaatc agacattact agacaatacg
     5221 aagccacttc gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat
     5281 atttttatcg tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact
     5341 tatcaagtac gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa
     5401 acagtcttgt cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat
     5461 tacgaattct tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag
     5521 atgtcattca gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac
     5581 aagtcttttc tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca
     5641 ttaggaatgt ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca
     5701 ggtaggatcg cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt
     5761 atagtacttt acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc
     5821 agcagttttt attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt
     5881 gttctgagtt tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta
     5941 aagggagtct gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat
     6001 acgctgttat atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat
     6061 ttgatgcaca gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt
     6121 ttttacaatc attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag
     6181 tgtcagtata ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca
     6241 aaaggaggaa gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa
     6301 cttttcggaa aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg
     6361 aaattttgaa gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga
     6421 tgagaaaaat caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc
     6481 caaagagatc aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt
     6541 ttcataaaaa actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa
     6601 gaaggaaaat aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct
     6661 aggggctaat attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc
     6721 cgccatgcta aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt
     6781 attcggtgat aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg
     6841 agcaaaatac tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact
     6901 tggggtgatg gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat
     6961 cattgttata gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc
     7021 aatgaaagaa atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga
     7081 aagtcgacac agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt
     7141 ggtcttggct ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc
     7201 gatcagcgga ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga
     7261 attttatagt ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt
     7321 agcttttgaa cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc
     7381 aacggagatt ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta
     7441 ctcggtcatc aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta
     7501 tatatacatt gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc
     7561 atgaattgaa gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt
     7621 tagcgaattt atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt
     7681 aatgaacaaa aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg
     7741 tataagctat ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga
     7801 tcctctctta tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt
     7861 tgcatctatg cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt
     7921 agaattacgc tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg
     7981 gacgtttttg gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt
     8041 aaagttttca actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc
     8101 aatgactgca tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt
     8161 agatgctgac cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca
     8221 gatgaaaagc gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt
     8281 catggtatta ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc
     8341 cggatttatg ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg
     8401 taaagatgtc aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta
     8461 aactatattt gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt
     8521 tcccgatgct ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat
     8581 atgtaccggt ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac
     8641 atctggatat tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt
     8701 acaaaacacg ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt
     8761 ctatgcttta aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac
     8821 tgatctatcc gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat
     8881 aaaaaagatt agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg
     8941 atttgtcctt ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag
     9001 ttgagggaat ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta
     9061 atcttctatg taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga
     9121 acaatcaaga tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat
     9181 tgttcctgat gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag
     9241 ccacaaaaga aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca
     9301 tgtttgtcat cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg
     9361 gaatcagtcc gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat
     9421 ctttgattgc tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc
     9481 tgcggcctta tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg
     9541 ctggtactta ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca
     9601 gtacagtcgc ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac
     9661 gcagaaaaga cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag
     9721 aaaaccgatc tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct
     9781 cagtcattac tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt
     9841 tccgtccaga gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc
     9901 caaataaatt gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc
     9961 caatattaag cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata
    10021 gtttttcgat tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg
    10081 gaccactata cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg
    10141 ctttgctagc ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt
    10201 tgaatcacca tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct
    10261 tccaagtaat cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag
    10321 cattcggtat tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac
    10381 ctttagcaaa gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg
    10441 cagtatgttt tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct
    10501 atgcaatgat ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc
    10561 aattcggaaa aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag
    10621 aataggttaa ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc
    10681 ttgtcgtttt tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta
    10741 taaggtctgg aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta
    10801 cagatagttc ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc
    10861 caatcccttt gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg
    10921 aatattatgg acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag
    10981 gggaagtttt gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta
    11041 tccatctttc ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca
    11101 tcagcggagt aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca
    11161 tggtagaagc tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac
    11221 atatcgatcg aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt
    11281 tagtacgagg aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca
    11341 aaccgatcct gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta
    11401 aaaaagcatt tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc
    11461 cagtcaaact ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga
    11521 aattgcagaa aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg
    11581 gtacacattt aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat
    11641 tcaaaaaaca taccaaaaat attccaacat catcttggac tttctacatg ttactaatga
    11701 gcgtgtaaaa gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa
    11761 tcctcacttc aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt
    11821 tacttattta ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt
    11881 aaaacataaa ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt
    11941 aaatacggat gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt
    12001 cgatatctca ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga
    12061 attaccaatc acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt
    12121 ccaagatgct tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat
    12181 tggattacaa gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga
    12241 agacatcatt ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt
    12301 ccgtatttta aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag
    12361 gggaagagaa gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa
    12421 aagcaaatga tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc
    12481 gcctccgaaa tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg
    12541 gaaggagatg ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa
    12601 cttccctata caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc
    12661 ttattacctg tagtagattc taataaggaa caagaccaag aattactaga agctatttac
    12721 caaacagata cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct
    12781 ttttctgcta aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat
    12841 gcaatccatt acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta
    12901 ttagagattc aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca
    12961 gcgctcgaac caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat
    13021 tgcgtcattg tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct
    13081 ttaagagatc ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca
    13141 gaacgtgcga tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag
    13201 attatccgtt tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg
    13261 cgttcttatt tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt
    13321 tatccaaaag ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa
    13381 gctttgtctg acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg
    13441 ggtcatcctc aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa
    13501 cgaaacatca ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct
    13561 gctgaagaac caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt
    13621 cctcctcaaa tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa
    13681 caaacaggag atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa
    13741 atcaagaagc aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa
    13801 gaaggtctca tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta
    13861 cacatgcaag taatgccaat agtagaatca cttagccggc aaattttaga gttactggat
    13921 aatgaacaaa cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc
    13981 aaagtagcat atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct
    14041 tctttagcgg tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc
    14101 gaagcggcaa aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca
    14161 ttgttgattt acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct
    14221 tataaagaat ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca
    14281 aggcaaaaca atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa
    14341 tccgcaacaa ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat
    14401 tatacaggca aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa
    14461 ggaatcctat ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac
    14521 ggtgaaagtc gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa
    14581 ctgattgccc gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg
    14641 agcttttcgt ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc
    14701 aagagggacc gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag
    14761 cagtaggcta tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag
    14821 acatcaatgg gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct
    14881 ggatcaatcc gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc
    14941 aagatataga agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac
    15001 atgaattagg gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc
    15061 aatggtttga agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg
    15121 tcgacggggt agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt
    15181 gggagtattc tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag
    15241 acttaaattg ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt
    15301 tggataaagg gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat
    15361 attcagtaaa agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag
    15421 aacatttgac cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag
    15481 caagtggtgt cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata
    15541 tgatattcga atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg
    15601 tccttaaatt gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga
    15661 acgctttata tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc
    15721 gtcccgattt ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag
    15781 gtacgccatt tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga
    15841 tcgatcaagt tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta
    15901 agacaagaga agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac
    15961 ccatgcaatg gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca
    16021 ctaatccaaa taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc
    16081 tgcaattcta taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt
    16141 cttataaaga atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag
    16201 atgaacaata tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa
    16261 aagaagcaga tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag
    16321 ctaaaggcat actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc
    16381 tcgttaaagt tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac
    16441 tcatttcaat aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc
    16501 acgtgagctt gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata
    16561 tgcataccga ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact
    16621 tcgacaggat cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt
    16681 gcaggaacaa cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact
    16741 aatgctcggt tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac
    16801 aactctttta acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata
    16861 tgtcctccaa cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag
    16921 tgtgcctcag ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct
    16981 gacaacttca ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg
    17041 ttgaagactt gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt
    17101 tcctggtata gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata
    17161 gccgatgatc ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga
    17221 tttgctgaaa gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc
    17281 ccactgcgga tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa
    17341 tccacccgtt ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac
    17401 aaatccattc gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc
    17461 aatcagcata gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa
    17521 taccgccaga ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat
    17581 gggcaacaaa ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat
    17641 tcgctgttat tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt
    17701 ttttatgaga aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc
    17761 cagccatcct tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt
    17821 tagcgagatg gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag
    17881 cttctgccaa attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg
    17941 aaatatacaa ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa
    18001 tgattgggat attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg
    18061 aattcaataa cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact
    18121 gtcgtttaag caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg
    18181 cccctataga agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat
    18241 ctgtttggat tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca
    18301 taacattttc ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat
    18361 tggcatattt ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga
    18421 tctttctttc cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc
    18481 gttgcacttt cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt
    18541 tcaagcgagg aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag
    18601 gagctaaagc aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg
    18661 ccaaaaaatc taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt
    18721 cgatcaaatc cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt
    18781 aatctccgct atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt
    18841 aataagtcaa agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta
    18901 ggacattccc aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca
    18961 tatctcttgc atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc
    19021 cgcattcact ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca
    19081 aaaatgtctt cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac
    19141 ccgaaaaagc ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat
    19201 gggtctctag tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg
    19261 cgtttgctaa aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga
    19321 atctggaatg cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga
    19381 aaactgtttt ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt
    19441 tagggatacc agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa
    19501 atgattgaga aacagacaaa gcaggttgtt ccattaaata aagaatagg
//
LOCUS       DACCDF010000050        19446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCDF010000050 DACCDF010000000
VERSION     DACCDF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.053"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            127..3654
                     /locus_tag="IVK51_001799"
     CDS             127..3654
                     /locus_tag="IVK51_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP6431382.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IVK51_001800"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IVK51_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP6431383.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IVK51_001801"
     CDS             7442..7783
                     /locus_tag="IVK51_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6431384.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IVK51_001802"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IVK51_001802"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP6431385.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IVK51_001803"
     CDS             9140..11560
                     /locus_tag="IVK51_001803"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP6431386.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKEINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IVK51_001804"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IVK51_001804"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP6431387.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IVK51_001805"
     CDS             12994..13731
                     /locus_tag="IVK51_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6431388.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="IVK51_001806"
     CDS             13745..14560
                     /locus_tag="IVK51_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP6431389.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="IVK51_001807"
     CDS             14573..15220
                     /locus_tag="IVK51_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6431390.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="IVK51_001808"
     CDS             15236..15892
                     /locus_tag="IVK51_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP6431391.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="IVK51_001809"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="IVK51_001809"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP6431392.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="IVK51_001810"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="IVK51_001810"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP6431393.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="IVK51_001811"
     CDS             18234..18752
                     /locus_tag="IVK51_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6431394.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="IVK51_001812"
     CDS             18859..19359
                     /locus_tag="IVK51_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP6431395.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6539 a         3340 c         4175 g         5392 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aagaaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggaca
//
LOCUS       DACCDF010000051        18760 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCDF010000051 DACCDF010000000
VERSION     DACCDF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.054"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..111
                     /locus_tag="IVK51_001813"
     CDS             <1..111
                     /locus_tag="IVK51_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6431396.1"
                     /translation="ISREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            124..510
                     /locus_tag="IVK51_001814"
     CDS             124..510
                     /locus_tag="IVK51_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431397.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            1061..1285
                     /locus_tag="IVK51_001815"
     CDS             1061..1285
                     /locus_tag="IVK51_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431398.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMIREAT
                     PVEIDYCKLVYCGCGRWKEDHVQKNISKYI"
     gene            1303..1731
                     /locus_tag="IVK51_001816"
     CDS             1303..1731
                     /locus_tag="IVK51_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431399.1"
                     /translation="MEKSDTKANRYKPTAAEKKLLEVLINPDNLGKPVQELCSLAKIG
                     RTKYYDAMSKDGFVDLVNETTMDLIKGKASDVLNATYKYALTEKGHQDRKMILTIAGI
                     YADKQELKHSGGVDIRKQYEKMSDEELEELVKRYEKINDS"
     gene            1709..3127
                     /gene="terL"
                     /locus_tag="IVK51_001817"
     CDS             1709..3127
                     /gene="terL"
                     /locus_tag="IVK51_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HAP6431400.1"
                     /translation="MKKSTTLNRVEYAVVQRELAIRKARKSFWLFCQLLYPEFYKDDR
                     LYLKDLCHTLQDFYEDKIDKKILVINMPPRHGKTFTARLFVLWMFGQNPKTKIITGSY
                     NQILSSLFAQQTRDGIMVENENVKAEYFKDIFPEVQIKYGDAAKGFWSLDGSEEKNYL
                     ATSPGGTSTGIGANYVIVDDIIKTAEEAYNERVLDSHWEWYNNTLAQRMERPRKQILI
                     MTRWSSNDLAGKMLTRRKNNVHQICYKAVQEDGSMLCNEIMTHDEYLDVVQEMNVDIA
                     EANYQQEPIDQKGRLYQKFLTYDTLPDNIIKIWNYTDTADKGADYFASPVFAETSDHD
                     AYIIDVMYTKEPMETTEIAHANMIIRNRVNKVRIEGNNGGRGFRRNSERITKERGYRG
                     AVFEDFHQSDNKLSRILANSAWVENHVYYPDDWATRWPDFYEAMVSYQREGKNAHDDA
                     PDAVTGIAETIIEADDFEFIAY"
     gene            3139..4674
                     /locus_tag="IVK51_001818"
     CDS             3139..4674
                     /locus_tag="IVK51_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP6431401.1"
                     /translation="MFYSGITLKKYKRLRTKYSTQIEEGLFDPNAFMEDMKPFFDDRE
                     RKYLAYTSEKNEIDSREKPKTKIIKVNNKIHAGMYSVVVDQAVNHFTGIPIKWDYDVS
                     EQKRTFIQRMRDKLLGNDISAPSTPEEFETLTRQLSTMRFGMLDPDTATFQGATGLAF
                     RLLEPVETNDGWELRASNIEPWKAEKYENGAVYIKEKYDPYQKKFYEEMKVVTPKSIF
                     TYARYTDYPGTVQGAFKTLGNVPNPLGVINLTEFKNNTNRYCDFEVAEEISDAIDRVL
                     SDQQNEIEQFKLAYMLITGADLSEDKAKKMMEQLGIINLKDPNAKAGYVTKDLQKDFN
                     EYHFDLLKKQFYTVCKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAEVMAAYLDFAEKVTIDPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMIDVESRQSYPDGEYGGGIVESNRNEVLDQTSRTGRFSKTQNGKSNAK
                     ETK"
     gene            4754..5632
                     /locus_tag="IVK51_001819"
     CDS             4754..5632
                     /locus_tag="IVK51_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAP6431402.1"
                     /translation="MMEFYSTSDQKKYREYVEKNYKSLKMYDAKYKEFIEEYFPSFDY
                     AKVNRLLQMRSDIFRILAESAIDADVNKYFSDRLEDILQRSYVSNANVFAQILNVDLP
                     NYLSKEELERYLNYPWSGKTFSRRLWGNISSLEQKLSNAITTSVASGEGVLVALRTMR
                     EDPEICDMFKREESKYNKAIENLVRTEYAKFAQDGIEKSYIETGVDEYNVLTAKDEKV
                     CSICGGKAKNNPYKLSEAIIGENRAPFHGRCRCTDVPNMPKLGKDIDEEYERLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            5634..5951
                     /locus_tag="IVK51_001820"
     CDS             5634..5951
                     /locus_tag="IVK51_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431403.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYENMGCIVVRTDYKEGK
                     SSN"
     gene            6000..6701
                     /locus_tag="IVK51_001821"
     CDS             6000..6701
                     /locus_tag="IVK51_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAP6431404.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMIMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            6716..7606
                     /locus_tag="IVK51_001822"
     CDS             6716..7606
                     /locus_tag="IVK51_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAP6431405.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            7629..7844
                     /locus_tag="IVK51_001823"
     CDS             7629..7844
                     /locus_tag="IVK51_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431406.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            7856..8197
                     /locus_tag="IVK51_001824"
     CDS             7856..8197
                     /locus_tag="IVK51_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431407.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8197..8568
                     /locus_tag="IVK51_001825"
     CDS             8197..8568
                     /locus_tag="IVK51_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431408.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8561..8956
                     /locus_tag="IVK51_001826"
     CDS             8561..8956
                     /locus_tag="IVK51_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAP6431409.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            8958..9335
                     /locus_tag="IVK51_001827"
     CDS             8958..9335
                     /locus_tag="IVK51_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431410.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9336..9944
                     /locus_tag="IVK51_001828"
     CDS             9336..9944
                     /locus_tag="IVK51_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431411.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            9944..10285
                     /locus_tag="IVK51_001829"
     CDS             9944..10285
                     /locus_tag="IVK51_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431412.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10527..13316
                     /locus_tag="IVK51_001830"
     CDS             10527..13316
                     /locus_tag="IVK51_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAP6431413.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13306..14046
                     /locus_tag="IVK51_001831"
     CDS             13306..14046
                     /locus_tag="IVK51_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP6431414.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14043..16805
                     /locus_tag="IVK51_001832"
     CDS             14043..16805
                     /locus_tag="IVK51_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP6431415.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            16818..17726
                     /locus_tag="IVK51_001833"
     CDS             16818..17726
                     /locus_tag="IVK51_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAP6431416.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            17726..18343
                     /locus_tag="IVK51_001834"
     CDS             17726..18343
                     /locus_tag="IVK51_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431417.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAESYTNERIPNFTFEKIGEV"
     gene            18347..18670
                     /locus_tag="IVK51_001835"
                     /pseudo
     CDS             18347..18670
                     /locus_tag="IVK51_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6693 a         2623 c         3977 g         5467 t
ORIGIN      
        1 attagccgtg aagtgggtta ttcagaaaga agcatacaaa ggatgaagtc ggaagctcta
       61 atagagtttg cagaagcgta taaacacgga agaataattg cttataaata gggggaccat
      121 ataatgtgga aagattatgt atcgttgaaa gagttgaaaa aagatcttgt tttcaaaaga
      181 attgtagaat ggtcagagag tgaattaatt ttagaagatg gaaccaaaat ggaagttgta
      241 tgtagcgaat cagattgctg cgcatgggcc gaaggtgaat tcaaaaatgt aaagcttgat
      301 gcagtgatta cagatataaa gatttttgat aaaggtaacc gtctctataa tggtgacgga
      361 catacctctt atgctgaagt cgttgtttat cataatcgta atgagatcgc taaagcagaa
      421 tgcacagcga atgatgggaa tggcggctac tattatagtg tttgtgcttt aaaggtcaaa
      481 gacaaacttt gtatagtgac tgacgcataa aaaaatggcg gttttttggc ggaatgatgg
      541 cggtttttag ccatttatca gtgatattat ggtagtgtcg aaagattagt gataggtctg
      601 agacaaaata ataataaaag gaacatcgtt ttattattgt ttcacaatta agcttcgata
      661 gacagcaacg gaaatattaa gaataaggat gtgaatttta actccttcta aattgttctt
      721 attatctatc atccgttgct gtctattgtt acatacagat cgcttaggcg gtctttttat
      781 tttggccgaa aacctacatt ttcgatggcc gattattgga ggaggaatag catgttcaaa
      841 ctatctgaaa tcattaagaa gtccgacgtt gagaaattag agatgttaaa aaagaagttg
      901 aagaaatagc agtctcttcg tgaggctgtt ttattttgct cacaaaaata gaccactatc
      961 gggtaatagt ggtcaggaat taaatgaaaa agatgttagg gttgttagct aagtataaca
     1021 tcataacgct tacaaaacaa tgcaagaagg ataaattatt atgagaactt actggtatgt
     1081 gtcattaaac aataaatatc cgaagccaat gaaaggacag cataggcgtg tagtcatgtc
     1141 tgttcaaatg aaggcgaagt attcgattgt agaaatgatc agagaggcaa cgccagtaga
     1201 gattgattat tgcaagctag tctattgtgg gtgcggccgt tggaaagagg atcatgtaca
     1261 aaaaaatatc agcaagtaca tatagagagg cggtgattaa ggatggaaaa aagcgatacg
     1321 aaagcgaaca gatacaaacc tactgctgca gagaaaaaac tcttggaagt attgataaat
     1381 ccagataact taggcaaacc ggtgcaagag ttgtgttcac tggcgaaaat aggtaggacc
     1441 aaatattacg acgccatgag caaagatggc tttgttgatc tagttaacga aacaacaatg
     1501 gatctgatca aaggaaaagc ttctgatgtc cttaatgcca cttacaagta tgcattgact
     1561 gaaaaaggtc atcaggatag gaaaatgatt ctgactattg ctggaatcta tgcagacaaa
     1621 caagaactca aacattctgg cggcgtcgat atccgaaaac agtatgagaa aatgtccgat
     1681 gaagaattag aagagttggt gaaacggtat gaaaaaatca acgactctta atcgtgttga
     1741 gtatgccgtt gttcaaagag agctagccat tcgaaaagct aggaaaagtt tttggctatt
     1801 ctgccagctt ctttatccag agttttacaa agatgatcgg ttgtatttga aagatttatg
     1861 tcatacgcta caagattttt acgaagataa aatcgacaaa aaaattttgg ttattaacat
     1921 gccgcctcga catgggaaga cttttacagc gcggctattc gttttatgga tgtttggaca
     1981 aaatccaaag acgaaaatta ttaccggatc ttataaccaa atcctgtcat ctttatttgc
     2041 gcaacaaaca cgtgatggaa tcatggttga aaacgaaaat gttaaggctg aatactttaa
     2101 agatattttt ccagaggtgc aaatcaaata tggagatgct gcaaagggtt tctggtcttt
     2161 agatggttca gaagaaaaga attatttagc gacttctcct ggtggaacat ccactggtat
     2221 tggcgcgaat tatgtaattg ttgatgacat cattaaaacc gcagaagaag cctataacga
     2281 gcgtgtttta gattctcact gggaatggta taacaacacg ttggcccaac gaatggaacg
     2341 gccacgtaaa cagatattga ttatgacacg ttggtcaagt aatgatttag ccggaaaaat
     2401 gttgacgaga cgaaaaaata atgttcatca gatctgttat aaagcagtac aagaagacgg
     2461 ctcgatgttg tgcaacgaga ttatgaccca cgatgagtac ttagatgtgg tacaagaaat
     2521 gaatgtagat attgcagaag ccaactatca gcaagaacca attgaccaaa aaggcaggct
     2581 atatcagaag tttcttactt atgatacgtt gcctgataac attatcaaaa tatggaacta
     2641 caccgacacc gccgacaagg gtgccgatta ctttgcgtct cctgtttttg ctgagacgtc
     2701 tgatcatgat gcttatatta tcgatgtcat gtatacaaaa gagccgatgg aaacaactga
     2761 aatagcccac gctaatatga ttattcgcaa tcgagttaat aaggtacgga ttgaaggaaa
     2821 caatggcggg cgtggattca gaagaaattc agaaagaatt acaaaagagc gaggctatcg
     2881 aggtgcagta tttgaagact ttcatcaatc ggataataaa ctgtctagaa tactggcgaa
     2941 tagtgcttgg gtggaaaatc atgtttacta tccagatgat tgggcgacac gttggccgga
     3001 cttttatgag gctatggtta gttatcaacg tgaaggtaag aacgctcatg atgatgctcc
     3061 ggatgcagtt acaggaattg cagaaacgat tatagaagca gatgacttcg agtttattgc
     3121 ttattaggag gtgggtcttt gttttattct ggaatcacat tgaaaaaata caaacgttta
     3181 agaacgaaat attcaacgca aatcgaagaa ggattattcg atcctaacgc ttttatggaa
     3241 gatatgaaac cctttttcga tgatcgagaa agaaagtatc ttgcttatac tagcgagaaa
     3301 aacgagatcg acagtcgtga gaaaccaaaa acaaaaatca ttaaggtcaa taacaagatt
     3361 catgcgggaa tgtattcagt ggtcgttgat caagcagtta accatttcac aggaatacca
     3421 attaaatggg attatgatgt ttcggaacag aaaaggacat tcattcaacg aatgagagac
     3481 aaattactgg gtaatgatat cagcgcccct tctaccccag aagaatttga aacactcacg
     3541 agacaattaa gtactatgcg gtttggaatg ttagaccccg atactgcaac atttcaagga
     3601 gcgacagggc ttgctttcag attactggag cctgttgaga caaatgatgg ttgggaacta
     3661 agagcaagca acattgagcc gtggaaagct gaaaagtacg aaaacggcgc agtgtacatt
     3721 aaagagaaat acgatccata tcaaaagaag ttttatgaag agatgaaggt tgtcacgccg
     3781 aaatcaatat ttacttatgc tcgatatact gattaccctg gcacggtaca aggagcattt
     3841 aaaacgctgg gtaatgttcc taatccttta ggcgttatca atcttacgga gtttaaaaac
     3901 aatacaaacc gttactgtga ctttgaagtt gcagaggaga ttagtgatgc tattgatcgt
     3961 gttttatctg atcagcagaa cgagattgaa caattcaagt tagcttatat gctgatcact
     4021 ggggcagatt tgtccgagga taaagccaag aagatgatgg agcaactagg gatcatcaat
     4081 ctgaaagacc ctaatgcgaa agccggctac gtgacaaagg atctacaaaa ggatttcaac
     4141 gagtatcatt ttgatcttct taaaaagcaa ttctacacgg tgtgtaaagc aattgatttc
     4201 aatgacgaag tctttaagtc gaatagttct ggggaagccc gaaagtggca aatcattagt
     4261 ttagaagcta agacaaatac caaagaacag tactttcgag aaggattgaa agagtgtgcc
     4321 gaggttatgg ctgcttattt ggattttgcc gagaaagtta caattgatcc ggaaaagatc
     4381 attttcacct tctctcgttc gcttccaaca gatttaagct acctagctga ggcattgcct
     4441 aagttggcac catacgtttc taagaggacg attcaaagtc agatcccgtt cgtcacagat
     4501 gttgattatg aaaacgaaat gattgacgtc gaaagcagac aatcttaccc agacggcgag
     4561 tatggaggtg gcatcgttga gtcaaataga aacgaagtac tggaccaaac gtcgagaact
     4621 ggaagattta gcaagactca aaatggaaaa tcaaacgcta aagaaactaa gtagtgtctt
     4681 tcctgatgcg ttgaaagaaa ttcaagcgga gttattatcc caagctgatt tacacgatat
     4741 tacctatgct gagatgatgg aattttatag tacaagtgat cagaaaaaat atcgtgaata
     4801 cgttgaaaag aactacaaat cactgaagat gtatgatgca aaatacaaag agttcatcga
     4861 agaatacttt ccttctttcg actacgccaa agtgaatcgg ctgcttcaaa tgaggtcaga
     4921 tatttttcga attcttgcag agagtgcaat agatgcagat gtgaataaat atttttcgga
     4981 tcggttagaa gatatcttgc aacgttcgta tgtatcaaac gcaaacgtat ttgcccagat
     5041 tttgaacgta gatttgccga attacttatc aaaggaagaa cttgaacgtt acctgaacta
     5101 tccttggtca ggtaagacat tctcgcgtcg tttatggggc aatatttcat ctctagaaca
     5161 aaagctatct aacgccatta caactagtgt agcaagtggc gaaggagttc tcgtggcatt
     5221 acgaacaatg cgagaagacc cagaaatatg cgatatgttc aaaagggagg aatcgaagta
     5281 caacaaggcg atcgaaaatc ttgtgagaac agaatacgct aaattcgctc aagatggtat
     5341 tgagaaatct tatattgaaa ctggcgtaga cgaatacaat gtcttaactg caaaagatga
     5401 aaaggtatgt tcaatatgcg gtggcaaagc aaagaacaac ccatacaagt tgagtgaagc
     5461 gatcattggt gagaaccgtg cgccttttca tggccgatgc agatgtacag atgttcctaa
     5521 tatgccgaaa ttaggaaaag atattgacga agagtatgag cgtttatttg gcgatttatt
     5581 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
     5641 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
     5701 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
     5761 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
     5821 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
     5881 ttgattggta tgaaaatatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
     5941 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
     6001 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
     6061 cgtggggcga aaggagaatg attatgaaac acaaatcatt aatgccaatg aatttgcaat
     6121 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
     6181 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
     6241 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
     6301 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
     6361 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
     6421 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
     6481 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
     6541 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
     6601 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
     6661 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
     6721 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
     6781 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
     6841 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
     6901 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
     6961 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
     7021 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
     7081 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
     7141 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
     7201 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
     7261 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
     7321 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
     7381 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
     7441 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
     7501 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
     7561 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
     7621 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
     7681 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
     7741 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
     7801 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
     7861 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
     7921 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
     7981 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
     8041 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
     8101 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
     8161 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
     8221 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
     8281 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
     8341 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
     8401 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
     8461 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
     8521 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
     8581 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
     8641 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
     8701 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
     8761 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
     8821 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
     8881 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
     8941 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
     9001 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
     9061 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
     9121 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
     9181 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
     9241 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
     9301 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
     9361 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaatttcc
     9421 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
     9481 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
     9541 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
     9601 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
     9661 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
     9721 gacagtttgc tctagattca gatgaaacgt caacgattga aggaagtaaa gtaagtttta
     9781 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
     9841 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
     9901 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
     9961 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    10021 tgctgaattt ttaccaggaa cagttgtaga agatgctgca ggagttatgg aagaaatgca
    10081 aacagcgact gataaacaat cagttaaaaa agctttaagt cgtgcttatt catttattgc
    10141 agatacttta tttgagggac aatttactgg tgaagattat tgcaaaggaa ttgatgctcg
    10201 tgagattgcc tcattgacag gaaaattatt gaagtctgtt actgcaggtt ttgatgaaac
    10261 ttatacagag acgaaaaaaa agtaagtgag gcgctcaagt caccttcatt taagtattcg
    10321 attacttacc gagaattaga tataaagacg caattactcg aagcaggttg gacgttacca
    10381 gaaattgaac gtactgactt ggatgagtta atgcgtcttt ttgcttttag agatgcggta
    10441 aaagagcatg aagatgttga gtactatgat aatttcactc aattttagga ggtgatactt
    10501 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    10561 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    10621 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    10681 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    10741 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    10801 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    10861 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    10921 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    10981 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    11041 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    11101 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggtttg tctcaaggta
    11161 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    11221 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    11281 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    11341 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    11401 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    11461 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    11521 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    11581 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    11641 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    11701 tagctgtttt cggttacttt atggcaacca atgaagagtt tagaaacaag gttatatcta
    11761 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    11821 ttgatacttg gaattctgct aaagaaatgg catcaaatgc agttgagggt gtcaaagatg
    11881 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    11941 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    12001 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    12061 gagaaaaatg gaatgaaatt aaaggttcta ttatgtcggt tgctggtcca ttgattacag
    12121 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    12181 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    12241 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    12301 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    12361 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    12421 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    12481 atttttttat taatctttgg attgatataa aatattttgc aattcagaaa tggattgaat
    12541 tgaagttttc tattattcaa acttggatcg atttaaaata taatgccatt actacttgga
    12601 ataatattaa acagtttttc aaagatactt ggaaaaatat taaagatacg gcatacaata
    12661 cgtggatttc tataaaaaat accatgatta atacatggaa taacattaag gactctttct
    12721 ggaatattgt tactggaatt gttaattctg ctgaaaatgc atggacaaat ctaaaaaatg
    12781 gtgtttcaaa tgctattaat cgggtaaaag aaatctttaa ttcgttaagg gaagtcaatt
    12841 tatttgaaat tggcaagaac atcattgatg gacttataaa tggtgtaaaa gaaaagtgga
    12901 atgctttgaa agatactatc aaaggtattg caggtaatat caaaaattgg attaaaggag
    12961 ctttagatat tcattctcca tcaagatgga tgcgtgatat ggttggtaaa aatattgttc
    13021 aaggtattat cgttggtatt gataaagagc aaagtaagtt agatcaaacc atgacaaatt
    13081 tagtaaaaac accgtctgtt caacctgtag taaatggaat aagtcaccta ccaattacac
    13141 aaacaaagca aaatatatct gctagtgaaa caaaagaaat tcatttacat ttaaatgttt
    13201 atggagattt acctgattca atggttcgga agattaccac aaaaattaaa acagaattga
    13261 caagacaaat gaaacgagat gctggtgcag taggaggaac attatatgca acttaagaga
    13321 ggacaatttt ttattaatca acattattct tctgaattta atgtgtatat tcaaaacagg
    13381 cctgcttctg tttcagctag tcgagtaatt gaattgagag agcgagaagg aaatgattct
    13441 atcattattg ataaaggcta ttacagaaat gtgactagaa agattgagtg ttactataaa
    13501 gcaccttcaa ttgatgtggt gcaggagtgg gaagatcgaa tcactgaatg gttagatatg
    13561 tcgtcttata gtgattttat tctttactat gatgaacaat atatttatca agctgtagta
    13621 atagaaggac ctgaatttaa aggaacaaga aaaacaggaa atattgtccc ttttgaattt
    13681 acagtgagca ttcgaccatt taaggaaaat tacaatggta gatttactat tcgacaaaca
    13741 gaaacttttg aaatttataa cccagagaag tattcttcaa aaccgcttat taaattgagt
    13801 ggttctggag atgcttcttt ttatattaat aaggacaaat atgatttgaa atcgttggac
    13861 agagaattat ttatagattc taaattagaa gaggcttacc gaaaattaga tggtaattta
    13921 gaacatcagg atcaagtcac tttattttta gattttccat ttttatatcc tggaaaaaat
    13981 gaaattaaat ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa
    14041 gtatgaaacc aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa
    14101 ttttaaagga ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag
    14161 agttggatta tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa
    14221 aagcaaagcc aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag
    14281 atactttttt aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac
    14341 gggtagtcac tcttgcggaa gtagactcta aaactggtca agaagcgata tcaattattg
    14401 aaaccaaaat ggataaaaaa tctgacatac gactttattc tgatattacg gccgtttcta
    14461 gcacaatctt tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc
    14521 ttcaatattg gggtggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa
    14581 gaggtcgcaa taatgttgga acgattcggt atggaaaaga tatgtctggt ttaaaggtca
    14641 aattagattg gacaggtata aaaacaagac ttattccata tgctgatcct caaagtgagg
    14701 taggtacgac taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc
    14761 ctgatgtgta tacagagcat gttcagttta cagaagaaca aggagtaaaa gacattaaga
    14821 gcttaaataa aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta
    14881 aagtttctat tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg
    14941 cgaagattag aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata
    15001 tttatcttga atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga
    15061 aattagaagc aggagacatt caagttgctt tttatcagca acaagctgta actattcagg
    15121 ataaattaaa agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt
    15181 caatgattac aggtcaaggc agtgcaggag gatatgtaag tttatggcca aaagaaaaac
    15241 cttccaacat ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa
    15301 tgaataaaaa tgggattgct ttttctaaga aaggatggcg ggggcctttt acttcagctt
    15361 ggacattaga tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata
    15421 tctttcaaaa ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc
    15481 aaatttggaa caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga
    15541 attctaaggg ttcaattgga acgattggaa caactgattc tgctggtaat ccttttcctg
    15601 gggcttctac tccaacccct attgaagata attctttagt tattcgtaca aatggagacg
    15661 gcaaatatat tttgatttct cctaaagtag gaaaaggatt agttttatta ggaaatggta
    15721 aagcaattta ttttggagac ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa
    15781 taattcctgg acaaggtggt ggtccatcgg gaggtggaga atctactggt gggtatccta
    15841 gtgaattaaa aacagatgca gaaaagagag cttggagaat ttacgatatt ttgtgtaata
    15901 acggatttac gaaacaatct gcatgtggaa tattggggaa cattcaacaa gaaacaggag
    15961 gaacttttga tcctgatact gttcaaatag gtggaccagc atacggatta gttcagtggg
    16021 atggttcttc atatcctctt gttggtccag caacatggga tggaaaagtt tatgttcaaa
    16081 acttatttaa tgctgcaggt attaaagaac caataacgag cttagatgca caagttcgat
    16141 tgcttatttg gacatttaca aatggacaat ggatgggcgt agtacaacct acgacggttg
    16201 atggatttaa ggcctgtact gatccaagac aagcagcata tgcttttgaa cgaaactatg
    16261 aacgtccggc agcgacacat cctgaacgtc aagattatgc agttaactgg tataacaaat
    16321 ttaaagattt aaaaccagga ggagctactg gagaagcagg actaaaacat ttagagtctt
    16381 tgattggaca acgaattggc aatggtcagt gttatggctt gtctgcagaa tattcaggat
    16441 atctgggtgg ttgtggcatg ggtgctggaa caaaatatgg tttaactcat gtgataggaa
    16501 atacttctgc agcatctgat attggtattg cctatgattg gtctgctgtt ggttggaaag
    16561 tgattcaaaa ccctagatat gatcaattag tagttggtgc aattattaat tgggcaagag
    16621 gtggacaagt gggttcatgg tttgcagacg gaacttatgg acatactggc gttatccgag
    16681 gcttagctaa tggtcgtatg caaacttatg aacaaaacac agagttaggt atgatttgtg
    16741 gaaagttaga tcgccagtat tatagtgcaa gtgcaatttc ttccattgtc ataccaccaa
    16801 aatagggagg tgatttaatg gcaaagtgga atgtcatatt aagtacaaca gaaccctata
    16861 attacgtggg gatgattcaa gttcgacaag gcaataaaaa cactgaagtt atggaagcta
    16921 caattgtcga aaatggtctt ccatatgatt tatcagaatg taaggtatat tttgaatcag
    16981 ttgttggcgg aaaatatcca gtccaattag aaacaagaat tgtggatgct aaaaaaggga
    17041 aaatcaaata tatttttgat aaatattcca tgcagtgttt gcatcgacaa acagccaatt
    17101 tcattatatt taaaggagaa gacttgattg gaacaactca agacttctcc tattttgtca
    17161 tcaatgctgt ttcaaaaaca gaaggagaaa tgggttctta ttggcaatca attgaagatt
    17221 taattgctga tatgacagat tttattaatg aaaataaagg cgattttacg gactggatga
    17281 atgaaaggaa agaagaattt gatcgctgga gagaagaaca ggagaatagt ttccaagatt
    17341 ggagagaagg acaagaatca gattatttgt catggtttga atcaatcaag gatattttga
    17401 agtctatcga tccaggtgga gtaatgttag ccgaattaat ggatgcacgt gttgatattc
    17461 aaggagttcg tcatgcgtct atttcagagc gcttgctggc agatatggat tatctatatc
    17521 agaaattacg agcaacactt ttcacgattg aatacggtga gattgaagtg actgatattt
    17581 tgcaggatga tctcttttca gataatcacg aagtcgaaaa agttggaact gtagaatttc
    17641 caattgaaga aggagctttg attattgcaa ctgttgatga tccaaaacag aatgttttca
    17701 ctcttgagaa agttggggtg atttgatggt taaagtaaag agaatgatgg agactgatga
    17761 atctggtgtt aaacgtcaat ttatgccaat tacacatgtt tctgcagttt taggcttaga
    17821 aaaaatgata tcaggacaat caaaagttct ttcggttaat ggtaaatatg gtgcagtcat
    17881 tttaacgaaa gctgatttag gattagagaa tgcaattact gaactacctt atgcaagcga
    17941 gacaagcgat ggtattctta ctgctgaaat gtttcaaaag atcgtaaatg gagaaggtgg
    18001 aacatatatt ttgccgattg ctactcctga acaattagga ggtataaaag ttggggaact
    18061 cttagaagtt actgaagaag gtgttttatc tgcaactaag caaacagact tcaatttctc
    18121 tgaagaatta aagggaaaat tagaatctct aaaaatatta aaagcaggtg caaatatatc
    18181 aattgcagaa gatggaacaa ttagttctac aggtggtagt gggactggtg gtgttaatca
    18241 gtcatatgta gatcaaaaat tccaagaagc tgtaaatcaa gcagaaagtt acacaaacga
    18301 aagaattcca aattttactt ttgaaaaaat cggggaggta tagaaaatga cagatatcgt
    18361 aaaaataaaa caaagcggtg tacaggttta tccacaaact cattggaatg ctatagaagg
    18421 caaaccaaca acggtgaagg gagataaagg cgatcctggc caagcagcaa caattaccat
    18481 aggaaccgtt tctagtggtt caaccgcttc tgttacgaat gtaggaacat catctgcagc
    18541 tagatttaat tttgttttac caaagggaga taaaggtgat cctggaataa atgccacaac
    18601 tacagctgta gctacaacta cagcaaatgg gttgatgtcg tccacagata aaactaagtt
    18661 ggatggaatt gcagctggag cacagaaaaa tccaggtaat gctacaacta cgacagcagg
    18721 tttaatgtca gcaaccgata aagtgaaact cgatggatta
//
LOCUS       DACCDF010000052        17687 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCDF010000052 DACCDF010000000
VERSION     DACCDF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.055"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(136..1716)
                     /locus_tag="IVK51_001836"
     CDS             complement(136..1716)
                     /locus_tag="IVK51_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP6431418.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2117..3493)
                     /locus_tag="IVK51_001837"
     CDS             complement(2117..3493)
                     /locus_tag="IVK51_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP6431419.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(3621..4739)
                     /locus_tag="IVK51_001838"
     CDS             complement(3621..4739)
                     /locus_tag="IVK51_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP6431420.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            4881..5315
                     /locus_tag="IVK51_001839"
     CDS             4881..5315
                     /locus_tag="IVK51_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431421.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5393..5926)
                     /locus_tag="IVK51_001840"
     CDS             complement(5393..5926)
                     /locus_tag="IVK51_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP6431422.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IVK51_001841"
     CDS             complement(6020..7198)
                     /gene="mutY"
                     /locus_tag="IVK51_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP6431423.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IVK51_001842"
     CDS             complement(7191..7988)
                     /gene="recX"
                     /locus_tag="IVK51_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP6431424.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8095..9477
                     /gene="rlmD"
                     /locus_tag="IVK51_001843"
     CDS             8095..9477
                     /gene="rlmD"
                     /locus_tag="IVK51_001843"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP6431425.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(9651..10229)
                     /locus_tag="IVK51_001844"
     CDS             complement(9651..10229)
                     /locus_tag="IVK51_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP6431426.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            10551..11021
                     /locus_tag="IVK51_001845"
     CDS             10551..11021
                     /locus_tag="IVK51_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP6431427.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11129..12355)
                     /locus_tag="IVK51_001846"
     CDS             complement(11129..12355)
                     /locus_tag="IVK51_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP6431428.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13109..13309)
                     /locus_tag="IVK51_001847"
     CDS             complement(13109..13309)
                     /locus_tag="IVK51_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6431429.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13443..14051)
                     /locus_tag="IVK51_001848"
     CDS             complement(13443..14051)
                     /locus_tag="IVK51_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP6431430.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14136..14606)
                     /locus_tag="IVK51_001849"
     CDS             complement(14136..14606)
                     /locus_tag="IVK51_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP6431431.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(14654..15568)
                     /locus_tag="IVK51_001850"
     CDS             complement(14654..15568)
                     /locus_tag="IVK51_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP6431432.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            15714..16517
                     /locus_tag="IVK51_001851"
     CDS             15714..16517
                     /locus_tag="IVK51_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP6431433.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            16695..17642
                     /locus_tag="IVK51_001852"
     CDS             16695..17642
                     /locus_tag="IVK51_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP6431434.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5364 a         3543 c         2911 g         5869 t
ORIGIN      
        1 gacatttcta tgattttaat atagtaaaaa gagccgtcac ggaaaatccg tcacggctct
       61 aaacaaaata tgagaggctg tttttgtttg gctattctta ttacttgatg cagggcagct
      121 tgtgtcaaga cttctttaca tcaaactttt taattttaca ttgggatatt tatccgcaaa
      181 ccagcgttct gcaaattggt tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc
      241 caagatattg cgacttgaac tcatcttctc atccaaatct tctggatcga tccagcgagc
      301 gattttgtgt cccattggag acatgattac ttcggcgtta tactcattta acatacgata
      361 ttggaatact tcgaattgta gctgaccaac agctccaatg atatattctt ccgttagata
      421 tgttttataa agctgaatcg ctccttcttg aactaattga tagattcctt tgtggaagga
      481 tttttgtttc atgacatttt ttgcagtcac tttcataaac agctctggag taaatgaagg
      541 gagttcttca taagcaactt ttagttttcc ttcgaacaat gtatccccaa tctgataatt
      601 gcctgtatca tatacaccga tgatatcgcc tgctactgct ttttcgacgt tttctctcga
      661 atctgccata aattgtgtga cattgcttaa tttcagtttt ttatttgtac gttccaacca
      721 gacatccatt cctcgttcga atgttccaga acagattcgg acaaaagcga ttcggtcacg
      781 atgggctgga ttcatatttg cttggatctt gaatacgaaa cctgaaaatt cttcctcata
      841 aggacttacc atctgatcat cttgtgtttt gtgtccataa ggagctggtg ccaattcaac
      901 gaaagtctct aaaaaagtct gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg
      961 tgtctgatcg ccacgagcaa ttttttcttg attaaatgca tctccagcct ctttgattag
     1021 ttctacttct tctaagactt gttcatataa ggaattttca gttagaggat gattttcggg
     1081 aagcagtcca tcttcatcca attctactaa tcgttcatcc tgataatttt ccggacgata
     1141 aaactctaca cgattatggt agatatcata aagaccttct agccctttac ccataccgat
     1201 tggccaattc atcggataag attcgatatc taatagctct tctaattctt ctaacaattc
     1261 caatggttca cgaccatcac gatctagttt gttgataaaa gtaaatatcg ggatgccacg
     1321 tttttttaca acttggaata atttctttgt ctgtgcttcg atcccttttg cactatcgat
     1381 taccatgacc gcactatcca cggccatcaa agtacgataa gtatcttcag aaaaatcttc
     1441 atgtcctggt gtatccaaga tatttactcg tttcccttga taatcaaact gcataacaga
     1501 actagtaaca gaaatcccac gttgtttctc gatttccatc cagtctgatt tggcaaaatt
     1561 acctgttttt ttgcctttta cggtcccggc ttgacggata gccccaccaa acaatagtaa
     1621 ttgttctgta atcgttgttt tcccagcatc cggatgggaa atgatcgcaa aagttctgcg
     1681 attgtccact tgttgcttta attttggatt attcataacc taactccatt tcttccattc
     1741 aattatttat attttctcta ggcacagatt gtccgatctc ttaaagaaag acaatttcat
     1801 atagggctgt gacaaaagcc gtgtagcagt ctctttttcc gagtaaacgg cgttctagag
     1861 acagcctatc agcaataagc ccacccaagc caaaaatacg aaaagctgtt tttgcttggc
     1921 tgcacttatt acttaatgca ggatagcttt ttcacaacct ttgtcaacta ctacaactga
     1981 atcagtatag catggattca gcgaatgaaa aagccaccaa accataagag tcaaatattt
     2041 ttttgtcagc tgactaaaag aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc
     2101 ataaccaagt ttccaattat cttcttggct cgtcatcttc gaattctttt ctgaagtatc
     2161 ttcgttcttc ttcaggttcc gcttctgttt cttccggatc atgaaaaatg acacggattt
     2221 taaggattcg agaaccttcc atttcttcag aaaccagcgt aatattatcg acatcaaacg
     2281 ataacttttc gccttcatcc ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg
     2341 tatctacatc actcatatgc agatcactgt caaaagcttc attgaactca tcaatcagca
     2401 tacgtccttg gatgatatat tcgtgttcat cgattttttc ataaagattc tcaacttcat
     2461 ctgattcatc atcgatttct ccgacgattt cttctaaaag gtcttctagt gttgctaacc
     2521 caacgacgcc gccatattca tctaataaaa tagccatctg attctgcgtc tttttcattt
     2581 catataacag atcatcaata aagaccgttt caggaacgaa taaaggctcc tgcatgattt
     2641 tcttgatatc taaattatca aagccgaatt tatgggcagc ttttagtaaa ttctttgtat
     2701 gaaggattcc gactacttta tctttgtctt cgttataaac agggattctt gaatagtttt
     2761 cggataaaac ttcattcaca ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat
     2821 ccgttcgtgg gaccatgact tctcgggcaa ctttcgtatc aagagaaaaa accccttgga
     2881 gcatttctaa ttcttcatta ttcaatacgc cttcgttttc aagcatataa cgcatttcat
     2941 ctctggtcat ttttgaatct tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac
     3001 ttgtggaagc agaaagcagc cagacgaagg gcttagctac tacaccaatc aagcgtatca
     3061 gccctgaagt gaactttgcg acttcttctg atttatttaa agcaatacgt ttagggtaca
     3121 attctccaaa aacaatcgat acataggtca aaatagccag cacaatgata tttgcaatat
     3181 tttttgccgc tgtgccgcca cctaataccg gagctaaccg ttccgataaa gtattagcta
     3241 gtgaagcacc agacaaaatg ttgaccaaag tgattcctac ttggattgtc gataggaaat
     3301 tatttggatt ctgcaatatt tttaaaagct tttgagattt cacatctcct tcatcagctt
     3361 tttgctcgat tcgattctta ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag
     3421 cattgatcaa tgtcaaaacg atcaataata aaatctgcgc taacagcgac tgactctcag
     3481 ggtcagcatt cataatcgca ttctctcctt ataaaattaa tttttagttt acgatagaaa
     3541 aaaacgtggc actatagcca cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa
     3601 aacaagtgta ctaaaactac ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc
     3661 ttgtcttctt tgacgatttt gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa
     3721 ggaacaccta agaagatacc aagcaatccg gcaatatttc cagctactag aagaacgata
     3781 atgatcgtta atggatggat tttcagtgat ttcccaatta cattaggata aatgatattc
     3841 ccatccaact gttggacgat tagcacaacc acacaacaaa gtaatgcttt taccggatca
     3901 ttgaagactg ttactagaaa tgcaggtgct agtcctagat aaggtcctaa atatggaatt
     3961 aggtttgtca atcccgcaat gaccccaaat aagaaagcat aatccacacc aattaccata
     4021 tatcctaaaa atgtgaaggt accaacgaat aaacattcga ttgcttgtcc gctaatgtaa
     4081 ttagccaatg ttttattcag ttgccccaat aactccacaa tttggtttct tcttttttct
     4141 gggaaaaact gttggatgtt tggcactagt ttttctccgt ctttcaacat atagaacaag
     4201 atgaatggtg ctgtaatgat tactacggtt gccgaagcga tcgttccaac gatcgaacct
     4261 aaactattgg atactccact aaggaattgc tgaatgattg tcccgtaaga aatgtctaat
     4321 tgctcaaaat atttattgat atctacttcc ttgaacaaag gcaattcggc tacctctttt
     4381 aaccagcttt ctacctgttt gatgaaatca ggcatattag atgccaatga ggaaatttgc
     4441 tgtactagac ttgggatgac actaagtaaa atccaaataa ttgctgcaac taaaaggagc
     4501 agcacaattg cgacagccca tattcttttg atttttgctt tcatcaacag cttgacaata
     4561 ggattcaata aatagtaaag aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa
     4621 aatgtcccga tcggttcgaa aataaagttt atttctgatg aaatcatgat caatgcagct
     4681 atgatcaata attcaagcga ccaaaacatt agtttagact gtctaagttt ttcaaacaat
     4741 agatttcctc ctttctttct tactagtata tattatattc gttaaaaatt cttttattct
     4801 ttttttattt ttgaaaactt gccatacttt ttatttgttt tattttccag tatactatac
     4861 gaaaggaagg tggctagacc atgaaagttg tacaaacaaa agatacaatg agcaatattt
     4921 acttagatgc tgtacggatc aggcatcaag tttttgtagt cgaacagggt gtgcctttgt
     4981 ctcgagaaat agacaaagat gaagcccatt gtatccactt cgtcctgtac tcagataaaa
     5041 aagaaccgca aggaactgta cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc
     5101 ggatggcgat tctttctgaa tacagacacc aagggttagg aaaaatattg attgaagaag
     5161 cagaaaactt tgctaagaat caaggctata atacgatttt attaggggca cagtccacag
     5221 ctgaaacttt ttacgaaaaa ctaggatata cagcttacgg cgatcctttt gaagatgctg
     5281 gcatgcctca tatttatatg aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa
     5341 acagcaagct tttgagaaaa atctctcact ggcttactgt tcttttttgt gtttacttac
     5401 gcgacaattg cttgtaacgg ttataccaaa tatcaatata gccttccgag aacgggccat
     5461 ctccattatt gatccagtca accagaatct tcacattttc ttttaggatg aagtcaatat
     5521 cgttcgggta attcatcatt ttgctgtgca tctcatattc atcgacatcc aatagtcgtt
     5581 tctcgccgtc aggaaatacc ttgatatcca agtcatagtc gatatatttc aaggcctcat
     5641 cgtccagcaa aaaaggtgaa gctagattac aataatagga aacccccttt tctctgatca
     5701 ttgctattac attgaaccag tattttttat gaaaataaac aatcgctggt tcgcgcgtta
     5761 cccatcggcg tccatcagat tcagtgacta atgtgtgatc attcacgccg atcatagagt
     5821 gttcgcttgt ttttaatacc atggtatctc gccacgttcg atgcaatttg ccgtcgtgct
     5881 tgtaactttg gatcgtcaca aactctcctt cttttggaac tcccatagca acccttcctt
     5941 aagcagcatt ttgtgctttg tgtttgggtg ttcattacac cgacatcata attattatat
     6001 caataaaaat gagctttgcc tagaaaggat tcctattgtt tcgatttttc tctaactgat
     6061 ctaccagttt catttggacc ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc
     6121 acttcgaagt tttgttctcg gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa
     6181 aaaggagaac atgccatttc agatgactaa aaatatgagt aacttctcct aaatgtcgtg
     6241 tctgccaaac aaagagttcc tgcttttcaa atgggagatt ttgctcttct tctgctacta
     6301 ggtcatcaaa cagatcgatt tctggctttg tttcccattc ttttttcaat cgctcatact
     6361 cctcttgtgt cacttccatc attggaaatg tccacatatt cgccagcaat ttttggctat
     6421 ctctttcttc aaaataatac gctccagaat gattttgaag agctgcactg atataataaa
     6481 cgtcttttgg ctttgctttt tttgttttta caggaaaact cgtttggatt cctcgtttgt
     6541 tagctaaaca aaaggcttgg atcggacaag tctcacactt tggagaagtt ggtgtacaga
     6601 tagctgaacc tagatccatc attgcttggt tgaattcacc tggatgcttt tcatcaatga
     6661 ttttccgcat cgcttcatca aagattttcc tgctagaagc ttttgcgatg tctgcttcaa
     6721 tgcaaaacaa tctgctgact actcgcatca cgttgccgtc aactgcaggt tctggaagtc
     6781 caaatgcgat acttgcaatc gcgcctgtcg tatatggtcc tattcccttc aatgagctaa
     6841 tttcttcagg cgtttgcggc atttccccat caaattctga catgatctgt ttagcagcag
     6901 cttggatgtt ccgggctctt gagtaataac caaggccctc ccatgctttt aaaagctttt
     6961 cttctggagc atttgctagc tcttcaattg taggaaacca ttccatgaaa cgataaaaat
     7021 aatcgatcac tgtgtctact ctggtttgct gaagcatgat ctcagagatc cagatacgat
     7081 aaggatcacg attatagcgc caaggcaggt ttcttttttc ttgttcatac cattgaatga
     7141 actgatcttg aaattctttt gtttctttat ctgtccattt ttcccattct ttattcactt
     7201 tcctcttcct tttccgtaat gaatcgctgg atcatatcca aatcaaatcc tttttgatat
     7261 aagctttgct ttacttttag atttcttttt ttaggttcaa accttaaatt tcttcgccat
     7321 agtttatctc cttgtttgac taaataatca tattctattt cttgatctgc ttcttgcgga
     7381 agatcagcta tcgcttgttg gatcacttct tgagaaaagc ctttcgtcat caatgtttgt
     7441 tggatttttc tcaataattc tttttggctt tttccgtgat tttttccaaa cgctttttcg
     7501 gctgtctgat gtgcattttg aacttggtct tcaaatgtat attggctgag cgcttgttca
     7561 atgatttcag gtttcaaacc tttttgctgc atctgctgcg ctaatttctt tggtcctttg
     7621 tcacttaagc gcatattcgt gcgcacataa ctttccccat agaccaaatc atcgacaagg
     7681 tccaattctt ttaagcgggc tacgatttta tttcgatctt cttgaaggat ttccttttct
     7741 tttaagtatg ttcggatttc tttttccgat cgcaactgat aacttaagta attcattgct
     7801 aggtgaaaac cgaaatcagc tccgctactc ttcttgatct cttggatagt ttcttcgtct
     7861 aattcccgtc cttttaataa tcgatagcgt actaatacat cttcagatac ttgtaaagct
     7921 tctccttcag ataaatctac gcgatagaaa gggcctttgc ctttactgat tctcgtaacg
     7981 gttaacataa acatcctcct cgaacatata ttctctatca taccacagaa aatccccgta
     8041 atcaggtatt atatgttaga ataaaccaca gaaaaaagaa aaagggagat acttatggca
     8101 gaaacaattg tcaaaactgg acaaacggtt cctttgaaaa tcaagcgttt aggcataaat
     8161 ggtgaaggaa tcggttattt caaacggttg atcatatttg ttccatatgc actaccaaaa
     8221 gaagaagtac tagtgaaaat cacgaaagca actcctcgct atgcagaagg agaattaata
     8281 aaagtcaaaa aaacaagcaa agatcgcgta gtggcaccat gtcctgttta ctatgaatgc
     8341 ggtggctgtc aattacaaca cttagcttat caagcgcaat tggattttaa aaaggatctg
     8401 cttttgcaag cattggaaaa attcaaacca gcaggctttc gatcttatca acttttgccg
     8461 acgatcggga tggatgagcc ttggcattat agaaacaaag cacaatttca gttacgaaaa
     8521 aataagcaga ctcaaaaaat tgaagctggt ttatacgaag caaactctca tcagttagtc
     8581 cctataacag actgcttggt tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt
     8641 caactattga ataaatttga tgcacccatc tatgatgaac ggacaaacag tggaatcttc
     8701 cgcacaatca tggttagaat cggtgttaaa actggggagt tacaagttgt ttttattact
     8761 cgcagtaaaa aattcccgca aaaaaacgca atgatccgtg agatcaatga acgactgccc
     8821 gaagttgttt caatcatgca aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat
     8881 gaaacgatcc acttatgggg aaaagaagcc attgaagaac aaatcaatga agtgactttc
     8941 gatctttctc ctcgtgcctt ctttcaactc aatcctcagc agaccgaatt gctttatggc
     9001 gaaggaatca aagcattaga tgtccagcct aatgaaacga tcgtggatgc ttattgcgga
     9061 gttgggacga tcggcttaag tgtagcaaaa caagccaaag aagtacgggg aatggatacc
     9121 gttcctcaag caatcgctga tgcaaaaaaa aatgcggaaa gattaggata tcacaacact
     9181 cgatatgaag tcgggacagc agagcaagtt ctgcctaaat ggttgaatga aggttttcag
     9241 cctgatggta tcatcgtaga tccaccacgt acgggtttgg ataacgcatt gatccaatca
     9301 ttattgaaat accctccaaa aaaacttgtt tatatctcct gtaatgtttc tacacttgcc
     9361 agagatctgg ttaatttaag taaaaagttc aagattgaat atttacagtc cgtcgacatg
     9421 tttcctcaga ctgcacgttg tgaagttgtc gtgaagatga cgagactcgc tgactaaatg
     9481 aaagaggttg tgaaaaagtc tgccctgtat caagtaataa gaacagccaa acaaaaatag
     9541 ctcttcatgt tttatgaata ctgtacttgt gaccgaagag ttaacaggag gacactattt
     9601 atttaaaaaa agggttgtga catagttttt gtcacagccc tttttgatta ttattggata
     9661 ttcacgtcta tcacttgata aaatgcattt gtcgtatcgc tgatattcca tactgctaaa
     9721 atcacattat atccttttcg atcctgcgga agtgtgatcg tttggttgac tgtttttggt
     9781 ggaacagttg ctttgtcgtc tatttttgtg atcaattcaa aatcactaaa tttcaaaggt
     9841 tgattaggat tccaagttgg tttggtaatg tagtaatccc aagtacttgt ccgatgttgt
     9901 gctgttaaat tccaagtaat atttaatgtt cctggattaa ccatgttttt atgccatctg
     9961 ttagatgtct gttcatctaa aggctcaaag ccgctgactc ctgcactagc aatttttcca
    10021 tcaatgaatg tatttttggt agcttcgata ctttgcggtt cccattgtgc tcttccgaca
    10081 ttttgattta agtttcctgc tgctgatgtt cctaggtatg cacggcttcc tggagaagta
    10141 atataaccat gcgcaaaacc tggaatgcta aaaccgaaca accctaaact aactaaccca
    10201 ataaacaata acgtactaat ctttttcatg tagaaacctc cttcatttga taaccacatt
    10261 gtaaacgctt tattgtatta acacaaacat tatgctttta taattttatg ttgaagtaaa
    10321 aaaggtgaaa aaagtaagat gcagattata acagaagtag acgagaaaag tacaaataga
    10381 aaacatgaga atcaaatcat atgatctttt tcaaaaacat cattcatata aacaaatgtt
    10441 caacttttta ataacaacaa ttatcaattt ttttcttact tcacaaacta aatacaggtc
    10501 ttgctgtgag ccttcatggt aaactactat tatagaaagg agttagtaaa atgaagatga
    10561 taaaagatat tcgtttttgg gtttgtgtta taggaattgt catattaggc tttttatcag
    10621 ggttgcttag cggaaatccc ggagaatatt actattcttt gcaacttcca ccatttgcac
    10681 cacctagctg gatatttggg ccgatgtgga ccttactgta tatattgatg ggtatttctc
    10741 tttatttgtt acttaatcac aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg
    10801 tgattcagtt catttttaat tttatatggt ctgctctatt ttttaattta agaaatattt
    10861 tcattgcagc aatcgatatt acacttttag ttattttctt gtcagtgctt atgtatcagt
    10921 tgtggctaca tcatcgcctg gcaatgtggc tcatgattcc ttattattta tgggtactat
    10981 tcgcaactct attgaattat tcaatttatt ttttaaacta agcgctaaaa agagacgtcc
    11041 tgtaatttaa cacatagaaa aagtgccacc taagttagat tttttgtcta atttaggtgg
    11101 cactctttat taaccaaact atttttttct agtacccact tgttgttcca tactcattat
    11161 tctggtcata accataacca ttttgttcct gtccagcgta aggatcataa ctttgagtat
    11221 cttgacttgg tgtatagtac tgacttgtat cttctgtttg ctgataatag gtattcggat
    11281 cttggtactg atcatatgtg ttttctgaat aggaagaata atcggtgtac ccttcactat
    11341 tcgatccatc accgtttgtc gaatcgccat cttccgaatc atcaaagaac atcattgaag
    11401 cattctcatt tttcaaatta ggtaatgtat cgcttgtttt tatatttaat tgtttcttca
    11461 gttcattttg gatcgaaaga agatcatttt ttcctaaaat ttgatagtag atatcattga
    11521 tcatttgtcc ttcgccttgc aactggtctt gtttgattgt ttcaaacgca ggggtgtagt
    11581 tcgtcgctac atcaagcatg tcatcccaat ctaagtccgt cgtcacattg ttctcaactg
    11641 cttttaaaat ttttctataa ttgcttacgc catctaaact taaaacttta ttgacgattt
    11701 tcgtcactac ttcccgctga cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc
    11761 gcattcgaga ataggctaaa cctttttctc cattcaatgt ttgttcacct actggtacat
    11821 ggacaccatc caaagtaaag tcaattttat tattcacttt gatgccaccg accgcattga
    11881 tcaaatcttc catgccatcc aaattgatcg ttacataatg atcaatagga atatcgagca
    11941 gttgctcaat tgaatccatg gccatttcga caccaccaaa tgcataggca tgatttaatt
    12001 tatctaccgt tccataacct acgatattcg tataaatatc tcggtccaaa ctgatgatcg
    12061 ttgatttttt ttgtttcgga ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt
    12121 ccgttcttcc taatccaccg gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt
    12181 catcaatgct tactctctca gaacgtttgg aggatattcg attagagttt ttagaaattt
    12241 tgtttactgt ttggctcgct tctcccataa atctgattcc gtatgctgat aatccggcaa
    12301 tcagcaaaac aaatacccct aacaaggtta atagaacttt ttgccatcgc ttcataatac
    12361 acctctgttt cacattttct aaaatattgt ctcatataac tgttcaatca gcaaattcac
    12421 tttttaatca taagaaaact ttaaaaaaca tttttgtgta acgattacta ttttttaaaa
    12481 tttctcaaaa ttcttaacta tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac
    12541 gcagttcgca atcgttttat ctttttttac gtattttttc cagaaaaagc gaacaacaag
    12601 gtattttcca aataatcgtg cagtaaaatg atcgcacata cctttcccat ctttttaaca
    12661 gtctttgcct accgttcaga ttatcattcg atttcacgaa aatttcttcc tatgactggt
    12721 ccttttgtta aacccatctt ctattcgttt tatttccagc tcactgcatg gctcttaaaa
    12781 tgactcgaac tagtgatttc attaccatca tcttttaatg gatagatatg ctcctcttca
    12841 aagtaaaacg attttctttt aaagtaatag aattgacatg gtaatgagta tgtatactag
    12901 tggtttcaca aatggtcttt tattcaacat ccaaaaactg aaatttcttt aaaatccatt
    12961 actaacgaag aaaatgttcc ttgcttgata attagttagg acatgtcaag tgaacaaatg
    13021 catttactta gccaaaaaat ctaaaaactt cataaataga caaaaagaca cgactctcta
    13081 agaagtcgtg tctttattca acaaattatt ataatttagt cacttcagca gcttgaggtc
    13141 cgcgtgcacc ttcaacaatc gtgaattcta cttcttgacc ttcttctagg ctcttgaagc
    13201 cgtctccttg aattgctgag aaatgaacaa atacgtcatc tccgccgtct actgtaataa
    13261 acccaaatcc tttttcattg ttgaaccatt taaccgttcc gtgttccata aatgtatagc
    13321 ctccaaaaaa gttcttgcaa gtaaccttgc aagaacatta tacccaattt agaaaacgct
    13381 agcaagcttt cgctctttta ttttccaaat tatttttaag actgatatga cggaggaaat
    13441 ggttattttg ctgcttcgaa acgtttgttt acttcatccc aattaataac attccagaat
    13501 gctgcaatat aatctggtcg gacattttta tatttcaaat aataagcatg ttcccaaacg
    13561 tccaaaccta gaattggtgt ctttccttcc atcaatggag aatcttgatt tgcagtagag
    13621 gtaatagcta attttccatt ttccattaca agccaagccc atccagaacc aaatcgtcca
    13681 gcggctgctt ttttgaattc ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc
    13741 gcttctttta tttctcctgt aggttcgcca ccagcatttg gtgccatgat ttcccagaaa
    13801 aatgaatggt tagcatgtcc gccaccattg ttacgtacag ctgtcttgat atctgttgga
    13861 atagcgtcca tatcagataa taattcttct attgtttttt cgcctaattc tgggtatttc
    13921 tcaattgctg catttaaatt cgtcacataa gtattgtgat gcttatcatg atgcagatgc
    13981 atcgtttctt cgtcaatata aggttccaat gcgtcatatg cataaggtag atctggtaat
    14041 gtgtaagtca taaaaaaatt cctcctcaat gttttcttta taatgtctac attacaaaga
    14101 gtaacaaaga gaaggaaatt cttcaattgt tatgcctagt cgtctttttc actttgacct
    14161 acttcttctt caaaaacctc agtttcttta aaaataaaca acttgctgaa aatatagttc
    14221 gctaggataa caataatttg agtgatgatt ttcgccacat actcattgct gttcataaca
    14281 ttgatcaaca ataccattgc ccccatgtcc aatccgtaag aaaggatacg gtagaagaag
    14341 aacttaccgc attcaataag tgcatgtgta aaattcggtg attttgaatg gaatacccaa
    14401 agtttgttcg ttacaaaagc aaacaataca gaacaaaacc acgaaataga attggataag
    14461 gccatactta aatcaaaata ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta
    14521 gccaaaccac caaaaaagag atagactagt atctcccaat atccttttgc ttctaaatat
    14581 ccttttaact gtcgatactg cttcatgagc caacctacct tattaaaaat tcttatttat
    14641 catactctat atttcaataa ctttcatctt tatttaaaaa aattaatttt ttttgatcct
    14701 ttgcctttgt ctctgcttgg atcgtcacat gtgtaatacc atattttaat tttaataaag
    14761 aaattatacg aggatatatc ttttcaatct cgcttagtag ctgatcttca aaatcaatat
    14821 gtccgtcgaa aataattatc ttttcgctat actgccaaat atggacatga tgaacattct
    14881 ttaccccatc gattgcttca atttcttgtt ccattttttc atagtcaata gcaggtgcac
    14941 tttgtatcaa aatagcggca gcttttttga tcaccattat cgcttctatc aatatatata
    15001 tcgaaatgat cagtgtaata actggatcga tccagtagat atcgaaaaaa cggatcaaaa
    15061 ttgctccgat aatcacactt accgaagaca gtgtatcact taacaaatgc aaataactag
    15121 attttatatt caaactttca tctgcttctt gtcgtaataa taatactgag aacaaattcg
    15181 cgagcaatcc aataactgct actgttagca tcaaatgact atttatcttt tctggtgaag
    15241 tgaaccgctt gaatgcttca acggttaaaa acaaggataa tatgatcaat atgaatgagt
    15301 tgatgaaagc actcaatatt tctaaacgct ggtagccata cgtacgtttt ttatttgctt
    15361 cttttcgggc cattttttgt gccccgtaag aaaaaataat tgccaatgta tcactaaggt
    15421 tatggaaagc atctgaaaca agagacaaac tccctgacat aatccctcca ataaattctg
    15481 ataaagtgat caccgcattc aaaaccacta ctccaaatag tttttttgat gatctctgtg
    15541 gctgtgtagg cactattcct tcctccattt tatttatatt ggatagtctt tagacaagaa
    15601 taacattttt ttgatatttc accaatcatt cagatgtttc cctaaagcac ttgtatttca
    15661 cttgatactt cgctagaatt gcaagtgatg acactactag atgaggagta cttatgacaa
    15721 caaaacaatt aattatcagt tcctttatac gattcaaaga cctaaaggag acgagaaatg
    15781 tcccattttg gaaagccatt gtctatcttt tagttttgag tataattatg gctttgccaa
    15841 tctcttttca agtttttcaa gtcttaaaca acattcaatt ggatgggcaa aagattgcta
    15901 ctcgcattcc tgattttaca atccaaaacg ggcagcttca aaccgaagaa aaagaaggat
    15961 tcatttacca aacaaactcc attatcttca catttgatcc agagggaaaa cgaacagaac
    16021 aggacatctc tactgatttg atggggaatt ttgttagtgt cgggatgtta aaaaacaaac
    16081 ttatcatagc tcttcctaat acaggcacga cttctgcttt gttgaacaat aaccaattag
    16141 aacttcccta caccaatgaa tctttaaaaa acttaacagg taaggagctc cgttcttttt
    16201 taagtaaagc atctatacca ctttggataa aagtcctgac atttatattt tctatttatc
    16261 ctagttttct gaatctgctg atcactttgc tatttaccaa cattgctgct tttctctatg
    16321 cacgctttcg actaacaaaa gctacttttc ttgattgttt aaaaacattg atctattcaa
    16381 tttcattgcc tactataatt gcaacaatct tgatggtctt tcttccttct tttgacacaa
    16441 gcgccttcat tgcttttgca ggaatcttta tttttgctca agctgtcaaa ggatgggcaa
    16501 aaatatcgat caattaagaa ataaagacaa ctcgttattt ctacttcaat agaccctttt
    16561 agaggttgtg acattttttt gtcacagccc cttttttatt tgaaaaatat ttctttctca
    16621 aaaaacaagc aaggcgcttc aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag
    16681 gcgggaaatt aactatgtca gaaagaatca tttatgcaga tcgtccattt cgtgatgaat
    16741 ttatcgaaga aatccatact atagcgcctg gttatatctt taaaacagaa ttacaaccag
    16801 aggatctaca aaatgtagag atcagtttgg gctggaactc agactaccaa gaaaaactat
    16861 tagcctcttc taccttgaaa tgggtccaat ctatctcagc tggtgttgat cagctacccc
    16921 ttgagacttt ttcaaagaaa catattttac tttcaaatgg tagtggcatc cacagtgaat
    16981 cgattacgga gcatgtctta ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac
    17041 aaaaagcaca gagtgacatg aaatggttag gttcatcggt ccattaccaa tctatccaag
    17101 gaaaaaaaat actaatcatt ggaactggcc atattggaaa aatgattgca aaaaaatgca
    17161 aagcattaga catgaactgc tatggaatca atacatccgg tcatccagca gaaggaatgg
    17221 aaaagacttt ttctttaaat caactaaagg aagttcttcc tgaagtagat ttcgtagtca
    17281 atatcttacc tctaacggag gaaacaactg ggctctttga tcagcatatg tttgaacact
    17341 ttgatccaaa atctgtattc atcaatgttg gccgtggtgc ttctgttaag acacaagatc
    17401 tggtgcaggc tttaaataac cagcaacttt cctttgcagc attagacgtt tttgaggaag
    17461 agcctcttcc acaagatcat cctctttgga aaatggacaa tgtactgatt acttcccaca
    17521 ttgcaggttt gacacctgat tttcaaaaga aattaatggc tattttttta accaatctga
    17581 aaagctactt tgccacccat gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt
    17641 aatagctgaa aacaatattt tcaaaagaac aaagagaagg tgacaaa
//
LOCUS       DACCDF010000053        17629 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCDF010000053 DACCDF010000000
VERSION     DACCDF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.056"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            287..694
                     /locus_tag="IVK51_001853"
     CDS             287..694
                     /locus_tag="IVK51_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431435.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVK51_001854"
     CDS             708..1109
                     /locus_tag="IVK51_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431436.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVK51_001855"
     CDS             1111..1482
                     /locus_tag="IVK51_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431437.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="IVK51_001856"
     CDS             complement(1518..1820)
                     /locus_tag="IVK51_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431438.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2069..2269)
                     /locus_tag="IVK51_001857"
     CDS             complement(2069..2269)
                     /locus_tag="IVK51_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6431439.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2574..3806)
                     /locus_tag="IVK51_001858"
     CDS             complement(2574..3806)
                     /locus_tag="IVK51_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP6431440.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            4063..4632
                     /locus_tag="IVK51_001859"
     CDS             4063..4632
                     /locus_tag="IVK51_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6431441.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4810..5250)
                     /locus_tag="IVK51_001860"
     CDS             complement(4810..5250)
                     /locus_tag="IVK51_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP6431442.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            5408..6172
                     /gene="map"
                     /locus_tag="IVK51_001861"
     CDS             5408..6172
                     /gene="map"
                     /locus_tag="IVK51_001861"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP6431443.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            6204..7127
                     /locus_tag="IVK51_001862"
     CDS             6204..7127
                     /locus_tag="IVK51_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP6431444.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            7203..8342
                     /locus_tag="IVK51_001863"
     CDS             7203..8342
                     /locus_tag="IVK51_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP6431445.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            8335..9135
                     /locus_tag="IVK51_001864"
     CDS             8335..9135
                     /locus_tag="IVK51_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6431446.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            9135..9962
                     /locus_tag="IVK51_001865"
     CDS             9135..9962
                     /locus_tag="IVK51_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6431447.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9940..10674
                     /locus_tag="IVK51_001866"
     CDS             9940..10674
                     /locus_tag="IVK51_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP6431448.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10774..11640
                     /gene="rfbA"
                     /locus_tag="IVK51_001867"
     CDS             10774..11640
                     /gene="rfbA"
                     /locus_tag="IVK51_001867"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP6431449.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11654..12226
                     /gene="rfbC"
                     /locus_tag="IVK51_001868"
     CDS             11654..12226
                     /gene="rfbC"
                     /locus_tag="IVK51_001868"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP6431450.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            12248..13276
                     /gene="rfbB"
                     /locus_tag="IVK51_001869"
     CDS             12248..13276
                     /gene="rfbB"
                     /locus_tag="IVK51_001869"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP6431451.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            13374..14225
                     /gene="rfbD"
                     /locus_tag="IVK51_001870"
     CDS             13374..14225
                     /gene="rfbD"
                     /locus_tag="IVK51_001870"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP6431452.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            14259..16292
                     /locus_tag="IVK51_001871"
     CDS             14259..16292
                     /locus_tag="IVK51_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431453.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            16336..17616
                     /locus_tag="IVK51_001872"
     CDS             16336..17616
                     /locus_tag="IVK51_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP6431454.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYGEQPDSAHSSRIQRYH"
BASE COUNT         5532 a         2966 c         3590 g         5541 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccctcaa taaacagtcg aagagaaaat aggcaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaacaga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccgacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaatgact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgcagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cagccgctga ttgataagac gctactcgat ggctatctac accagcaaat
     3481 gcatcgggat cggcagaaac aacactgatc ctgcttgctc ctttttcaag ccattcatcc
     3541 gcttgatcga ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga
     3601 aattctcttt ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta
     3661 taagcttctt gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca
     3721 actgtgtgtc ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt
     3781 agaagttcat taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa
     3841 ctattagcat gataccattt aattatgatt caaacaatga ggcctttaaa taaaaaaaag
     3901 aggttatttt tttaaggtta agtttcatga tatatgttac tacttgctat ttcagcttgt
     3961 gaagaaaatc aaaaactaat tttgcatatc aaaaaaaaca tgttacaatg aaattaatca
     4021 atggtggaag cgttataaaa aaattaagga gattaaaaaa atatggcaag aaaaaagacg
     4081 attactagag aacagatttt ggcagctgct tatgaagtag ctgccaccga tggatttaca
     4141 cgatttactg cgagaaacat cgcgaacaaa atgaattgtt ccacgcaacc gatttatctt
     4201 gagtttaaaa atatggatga cttgagacaa gctctttttg agcagatcta tgatcattta
     4261 caatatgaag tgttcactgt tgagcataca gggaatacga ttatcgatct tgcttttaat
     4321 tatatccatt ttgcaagaag agagaagaag ttgtatcgtt cactgtactt agaagaatat
     4381 ggtggaggaa aagagatgca gaatttctct tatacttact ttgtgaatac agtgaaacgc
     4441 gatccagctt atgtgaatct ttcagaagag cagatcgaat ctttgtataa tgggacatgg
     4501 atcatagcta ctgggatcgc agctttgatg tcttcaagca tcatccatcc aactgatgaa
     4561 cagattgaaa agatgataca agacagtata gatgctatct tgaaattcaa agaaccaata
     4621 gaaatcgatt gaatatactc aaaagttcca caaatcagta gataacaaga gatgtgtcaa
     4681 aataaggaaa agttcgtttt tcttacagcg tatgcatttt taactcccga agaactcttc
     4741 catgaatgca atgaataaaa gagccgtaca gattgaataa atctggatgg ctcttttatt
     4801 gcaagtatat taaatttttg cgactagttc tttagcgaaa gcttctaaat tttcgatatc
     4861 ttcttcttca gcattcagat ccactttaac attttcggct ccttttgatg cgcctgtttt
     4921 tgcaaaagca gcatcgaaat catccaccga tttgcagaat tcatcgtaaa aagtatcacc
     4981 agatccgcag acaccgtaaa tttttcccga tagatccaat tcttgcaagt cttcataaaa
     5041 gtctacgatc tcatcaggca gatcgccatc atcgtatgta taagtagcta ctacgcaaat
     5101 atctgcctcc tcgaattcat ctgcatctac ttgggtacat tcgttgattt cgacttcgat
     5161 atccagattt tcaaatgctt ctgcaacaat atcggcgatt tcttcagtgt ttcctgtcat
     5221 gctagcatat acgatttttg ctaaagccat gatgtgcctc ctcgtttata attatttaac
     5281 aattatgaat tataccaaaa catcattgaa aagaaaataa aacctagaga cacctgggga
     5341 atgtcatttg gctaaatgta tgccgctatg ttaaaatgag agggatttgt aaaaaggaga
     5401 cgaaaatatg attactttaa aatcagcacg tgaaattgaa atgatggcag aatctggagc
     5461 tttattagca gatgtacata aaaatctgcg tgattttata aaaccaggaa tcacaagttg
     5521 ggatattgag gtatttgtca gaaactatat cgagagtcat ggcggaattg ctgcgcaaat
     5581 tggctttgaa gggtatgaat atgcgacttg tatcagtata aatgatgaaa tttgtcatgg
     5641 attccctcgt aaaaagcctt tgaaaaacgg tgatttggta aaagtcgata tgtgtatcga
     5701 tttgaaaggc ggcatttctg attcttgttg ggcctatgta gttggagaat ctacacctga
     5761 gatcgaccga ttgatggaag taacgaagaa agctttgtac atcggtattg agcaagcaca
     5821 agtagggaat cggattggtg acatcggtca tgctatccaa acttatgtag aaggcgaaaa
     5881 tctagcgatc gttcgtgatt ttattggtca tggcgttggt cctacgattc atgaagaacc
     5941 agcagttcct cattacggag aagctggaaa agggttgcgg ttgaaagaag gcatggttat
     6001 cacgatcgaa cctatggtta ataccggaac ttggaaaatg aaaatggatc ctaacggctg
     6061 gactgcgtat acaagagacg gcggactttc ttgccaatat gaacatacat tggctattac
     6121 taaagatggt ccaagaattt tgacatcaca aggagaagaa ggcacttatt gatctatcca
     6181 gtcaataggt acgggagagg tacatgaaag tttggaacaa aataaaacaa aatgaaaatc
     6241 tgatgcgctt tattgagacg actcaaaagc gcatcactga ttcagaaatg agtacgacct
     6301 ctgtcgtcgt tgcttattat ttactcttgt ctttatttcc attgattatt gcttttggta
     6361 atattttacc gtttctccac atcgatcaag agactgtact gacctattta cgagaagtga
     6421 ttcctgagac cgtttaccag tttatcggtc ctgcaatcaa agaccttttg acacaaagtt
     6481 caggaggctt gttatccgtt tctgcattag cggctttatg gtctgcaagt caaagtatca
     6541 atgctttgca aattgcgatg aataaagcgt atggcgtaga aaatagaaaa aattttttga
     6601 tcgtccgttt cttctcacta attgtcattg tcttgttcat gattgctatt agtggggtaa
     6661 ctatcgttct tgggttggga cagctgattt tggatgctat tcagccaatc gtacaaatac
     6721 ccgtgaactt tatcgatcag ttccaaactt tgaagtggcc aatcacattg gtagctttat
     6781 ttgtcatcat gttcttgatt tatttaattg tccctaatgc tcagttgaag ttgaagacga
     6841 tcattccggg cacgatcttc gctactgtcg gctggatgct gttatcacaa gtgtttagta
     6901 tctatgctag gtattttgca acgaggatca gcggttatca gatcatagga agttttattg
     6961 tactcatgct ttggcttaat tttgctgcta cgatcatcat tttgggcgga atcatcaatg
     7021 cagttgttca agaatacgtg acgggtcatc aaatcaaaaa acgtcgagat ccaagccgaa
     7081 aatggttcaa taaattgaaa actaaatttt ctcataaaaa aaagtagaaa caagagttct
     7141 tattgtaaat ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc
     7201 atatgcttgt ttcttttagt atggttataa gattttttct gacaatttta ttatcactga
     7261 tattaacacc tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg
     7321 aaagaagaat aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat
     7381 ttgttatatc gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga
     7441 tcttgattgc ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga
     7501 cacctaaaca aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag
     7561 atatacgtat tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta
     7621 gttttcccgt aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg
     7681 gtttggatgg gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg
     7741 gatatttttt cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag
     7801 caagtattat cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata
     7861 caggagctct attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg
     7921 tgacttttgt ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt
     7981 tttttgctat cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac
     8041 atcttcatca ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt
     8101 atgcgatggc tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg
     8161 gaacgatcat attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg
     8221 gtctgatcaa tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag
     8281 agtaccgtac gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat
     8341 aacaaattaa accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt
     8401 cggacactag ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt
     8461 tttgacaaca attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc
     8521 tacatcgata tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat
     8581 ctgatagcta gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa
     8641 gagacagtta cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca
     8701 cctaagattc ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt
     8761 tttgattacg ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga
     8821 ctggctgtgt ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc
     8881 tcaggtagtt ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag
     8941 cgtttcttca tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac
     9001 aagttattgt acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga
     9061 aatgcaaaac tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg
     9121 tggcgttttt tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat
     9181 tcagtcaatc tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc
     9241 ttattattta cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata
     9301 tcgtttatca tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt
     9361 ctcaagcaga tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg
     9421 acgattattt tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc
     9481 cgatgatttt ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc
     9541 gtacagctca agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt
     9601 caggtgttgc ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt
     9661 ctttagattt tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg
     9721 agatccttcc tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa
     9781 gtaatcgata ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa
     9841 ggcatttatc cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg
     9901 tcaaaaaccg gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt
     9961 gaattcgtgc gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag
    10021 ttagatagta ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg
    10081 tcagtagatg atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag
    10141 cttttttcgg ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg
    10201 aaaggagatc taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa
    10261 aagatcactg actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc
    10321 attgtggaca gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa
    10381 gaaggatggc ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg
    10441 tcttctttaa aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg
    10501 attggtttgc tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga
    10561 agacatgatt taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct
    10621 tggcgtgttc atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag
    10681 atgtttaaat ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt
    10741 gaaatttgaa atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc
    10801 agcggaacac gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat
    10861 gacaaaccaa tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt
    10921 ctgattattt caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat
    10981 gatttaggac tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg
    11041 tttatcattg gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat
    11101 atttactatg gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc
    11161 gcgacagtat ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat
    11221 gagaatatgc aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct
    11281 gtaactggcc tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct
    11341 tcagaacgtg gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa
    11401 ctatccgtag aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca
    11461 ttgcttgaag catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct
    11521 tgtttggaag aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt
    11581 gcacagccat tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa
    11641 ggagtcctta cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat
    11701 ggacgtcttt ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc
    11761 agaacatgga ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg
    11821 tgtccttcgg gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt
    11881 agtcactgga gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg
    11941 tcaatgggac ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg
    12001 atatgcccat ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa
    12061 ttactataat gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga
    12121 ttggccaatt gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt
    12181 aaaagaattt gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa
    12241 ttttcttatg aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt
    12301 tcattacgtg gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta
    12361 tgcaggaaac aaagagaact tggctggact tcctagcgat cgcgtagaat tggtcgtagg
    12421 agatgttgca gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca
    12481 ttacgcggca gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac
    12541 gaatatcatt ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca
    12601 tcacgtatct actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca
    12661 tggggaaggc gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata
    12721 ttcttctaca aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt
    12781 acgtgcaacc atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt
    12841 tattcctcgc caagtaacga atatcttaag cggtattcgt ccaaaattat atggcgaagg
    12901 aaagaatgtt cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt
    12961 gacgaaaggc agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa
    13021 gaccgtgatg gaattgatct tagaaatgat gggtcaacca aaagatgcgt acgaccatgt
    13081 aaacgaccgt gcaggacacg acttgcgtta tgcaatcgat tcgacaaaat tacgggaaga
    13141 gttaggatgg aagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg
    13201 gtacgaagaa catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc
    13261 caaaaatgga caataataca gagaaaatgt cactgtatta atggaaaaac acatgtgagg
    13321 caggcgctcg cctgtctctt ttttaaaata aaaatcaagg agcggataat catatgtttt
    13381 tattaactgg aggaaatggg caactaggta cagaattgcg ccatttatta gatgaaaaaa
    13441 atctgcaata tgtttcaaca gatgcaaaag acttggatat cacagatgcc gagaaaacaa
    13501 tggcttacat cacagaatta aaacctgatg ttattttcca ttgtgcagct tacacagcag
    13561 tagataaagc agaagaagaa gcaaaagaat tagatgagaa gatcaatgtt gatggtacaa
    13621 gaaatgtagc acttgcagca aaagctgtga atgctcaatt cgtctatatc agtacagact
    13681 atgttttcga tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac
    13741 taaatgaata tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag
    13801 actattatat catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca
    13861 cgatgcaacg cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc
    13921 gaccaacttg gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac
    13981 catttggtat ttatcatttg tcgaacgaaa atagctgttc ttggtatgaa ttcgcaaaag
    14041 aaatattgaa agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa
    14101 aagcaacacg tccgcagtat tcagtcatga gcctgaaaaa gacagaagag ttaggatttg
    14161 tcataccaac ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata
    14221 agtaatttcc ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg
    14281 cacgacgaaa agaaaagcca aaagcgaacc aggcagattc gaatttggtg cttgacctgt
    14341 ttctaaaact ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt
    14401 taaacggaag ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa
    14461 aaactgatgt gatttttgga cagaatcggg aggtgcggtc agacgaatgg ctggtgcaga
    14521 caccttttta cctctcacaa gctgaagagg actttccgtt ggtgaacgag gattattcat
    14581 taaacggaca aaacatgatc attgcgtata attctcctgc aaaggatatt acggttatcg
    14641 gaaaaccatt caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt
    14701 gggcgatgaa attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga
    14761 agagaaaaaa agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc
    14821 agtggtggtt catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgactg
    14881 ctttttatca ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt
    14941 tggtggttat tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc
    15001 tggcgtactt gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt
    15061 catgggatct gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca
    15121 tggttcattt ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc
    15181 caggaaaacg ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta
    15241 caaattggaa aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt
    15301 tctatcactt ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag
    15361 atagcgaaca aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt
    15421 ggattaccgt tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc
    15481 cattaagagc catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg
    15541 cttatatttg gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa
    15601 atgcatggtc ttatttctat gcgattacgc attcgaaaat agataattat ttttcggatt
    15661 ttgaaaccat cgcagttgct gtgttgggaa cagtgatttt agcatttgca ttatttggct
    15721 ggagacgctt gttttacctc gcttttgccg ccttagttat cgtatctggc tttttcgtca
    15781 atccagtcgt agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa
    15841 aaatcgatca acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca
    15901 ccccaacatt gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat
    15961 ggaaggaaat cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa
    16021 gagcttttat cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg
    16081 tcacgttgaa ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac
    16141 cattaactga ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata
    16201 aagacggtta tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac
    16261 aaactcagga aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata
    16321 ttagtagaaa ggagagtgga gataattcaa gcaatcgtac aaaaatttgt caacaaaatg
    16381 agtgtcggtt tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcagggtta
    16441 ggttttccag caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg
    16501 gctattttgc tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt
    16561 gttgcgcttg cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta
    16621 tttggagatt tactgtttcc tttgattatt ttattttgtt tagtaattaa atggatcgag
    16681 tggacaaaat gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg
    16741 ttatatcgtg tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc
    16801 aattcgcttt ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt
    16861 gctttattga tcagtggatt tgccagttct tttgaacgat ggtatgtacg aatcatcggt
    16921 gcagtagcag ttgttgcagc ttttctcggg atttgggtct gccaatctag aggtgcatta
    16981 gtcgcgttga ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt
    17041 cgtgcgccat ttattgctta cacggttacg attcttttag ctttgccgat ttcttatctg
    17101 gcggctgtct ctgaaaaagt caatttgttt accggtcgag aagatatttg gcataaattc
    17161 tatcagacgc taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc
    17221 caacgaggaa accaattttt aggaaaccac aatagttata attctatttt gaatatctat
    17281 ggattgatag gatttgggat tgctactttg ctgcttatcc tttttattgg tcgcttaacg
    17341 ttgaaagcag atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg
    17401 atgcagtcat ttatggagga tacattgact tcatacccat gggttccgat tatttatatc
    17461 ctgttggcta tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt
    17521 atggaagagg catatgacga tatgacttat gatgatacat cttatgggga acaaccagat
    17581 agcgctcatt cttccaggat ccaacgctat cattgatagg cacaaaata
//
LOCUS       DACCDF010000054        17516 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCDF010000054 DACCDF010000000
VERSION     DACCDF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.057"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            100..2427
                     /locus_tag="IVK51_001873"
     CDS             100..2427
                     /locus_tag="IVK51_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6431455.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2553..2882
                     /locus_tag="IVK51_001874"
     CDS             2553..2882
                     /locus_tag="IVK51_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP6431456.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            2922..3926
                     /locus_tag="IVK51_001875"
     CDS             2922..3926
                     /locus_tag="IVK51_001875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP6431457.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3923..5352
                     /locus_tag="IVK51_001876"
                     /pseudo
     CDS             3923..5352
                     /locus_tag="IVK51_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            5558..6730
                     /locus_tag="IVK51_001877"
     CDS             5558..6730
                     /locus_tag="IVK51_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP6431458.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6892..7347)
                     /locus_tag="IVK51_001878"
     CDS             complement(6892..7347)
                     /locus_tag="IVK51_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431459.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            7936..8358
                     /locus_tag="IVK51_001879"
     CDS             7936..8358
                     /locus_tag="IVK51_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6431460.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8524..9717)
                     /locus_tag="IVK51_001880"
     CDS             complement(8524..9717)
                     /locus_tag="IVK51_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6431461.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            9941..10318
                     /locus_tag="IVK51_001881"
     CDS             9941..10318
                     /locus_tag="IVK51_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6431462.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10306..10644
                     /locus_tag="IVK51_001882"
     CDS             10306..10644
                     /locus_tag="IVK51_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6431463.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10778..11221
                     /locus_tag="IVK51_001883"
     CDS             10778..11221
                     /locus_tag="IVK51_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP6431464.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            11483..12298
                     /locus_tag="IVK51_001884"
     CDS             11483..12298
                     /locus_tag="IVK51_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6431465.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            12308..12970
                     /locus_tag="IVK51_001885"
     CDS             12308..12970
                     /locus_tag="IVK51_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP6431466.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            13175..14839
                     /gene="alsS"
                     /locus_tag="IVK51_001886"
     CDS             13175..14839
                     /gene="alsS"
                     /locus_tag="IVK51_001886"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP6431467.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            14852..15562
                     /gene="budA"
                     /locus_tag="IVK51_001887"
     CDS             14852..15562
                     /gene="budA"
                     /locus_tag="IVK51_001887"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP6431468.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            15692..16189
                     /locus_tag="IVK51_001888"
     CDS             15692..16189
                     /locus_tag="IVK51_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP6431469.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            16374..16634
                     /locus_tag="IVK51_001889"
     CDS             16374..16634
                     /locus_tag="IVK51_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431470.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(16996..17235)
                     /locus_tag="IVK51_001890"
     CDS             complement(16996..17235)
                     /locus_tag="IVK51_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431471.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         5944 a         3045 c         3473 g         5054 t
ORIGIN      
        1 aaaaaagccc caaactttct atacatcaat aaaataataa aaattaaatt tagaataacg
       61 tgtaaaggat tatttgatga ttttttattg agaggaggga tgaaaatgat taagaaaact
      121 caaaataaaa tccaacgtat atgtcaaaaa cattttgttt ctagactcaa agctgtatct
      181 aaaagaaaat acttacatcc cattatgatt tcgatcatct tgatttttac tttcttaggg
      241 agcttatcaa gtatttatgt aaacgtttat acagaaacgt caaattttgc taaccaaacg
      301 acagaggaga caaatagtcc accctattct gaaatacagt ccatagaaag caccaatcag
      361 tctagcaaca acgagacaac ctcaaccaca tcctcgactc aactgattga tccgacagaa
      421 aaagaaatca cagcagcaaa agcagcaggg gagaaagctt attcagaaac aggtagaagc
      481 cagacattta gtactgttgc ggccactgcg aatttcaacg gacataccat accagatgga
      541 tatacagtga tatatgccaa agatggagtc attgttgcgg ttaacggatc gatgagtgcg
      601 gataaaactg cgttacaagg cgtaaccaaa gcaatcgctt ttattgataa ccgtccagat
      661 atccataatc tgtttattgg ctccacagta aatggtacta ctatcacatt cccaaaaagt
      721 atctatatgg gggtggctac cgctggaaat acgatcgatt cagttacacc caatgcggat
      781 aaaagttatt cttttacacc ctcaacatgg ggagggcttc ctgccggagg aggtatgagt
      841 actttttcaa tcggtataca gttagcaaat gatattccat tcacggggta ctcgatcaaa
      901 aatgggcaga tcgttgatgg taagggaaat gtttcaaatt ttccaaccta tcaagcagca
      961 acggttcagg tcaatgcgaa tcggacagga actttttctt atacagtcaa tggtggagca
     1021 gcaaaaatcg gaacgtacgg gactacatta tctggaatcc atgtgggtga caaagtggtt
     1081 atcactccaa cagcagtaac gggctattct tttgcacaaa caaacggttc tttcacagcc
     1141 ctagaaagta acgtcgatac ggtgacctat acaggcaaac aaacaccaat cactattaag
     1201 tatcaagact attttggaca gatgattgct ccttctaaaa taatgaatct tacttacgga
     1261 agtgctgcac aagacctgac gactaatacg cctactatca acggatatac ctttacagct
     1321 gtttcagcga cagaaacaaa gaaccaatca gcaatgagtg ttttagctag tttagataca
     1381 aatggaaatg taatcgtaaa ggacgcaaat ggaaaacaga tttctgaagt catcctatat
     1441 tataagacaa atgcaacagt atcaatcaat gcaaatggca gtaagtacta tgatggtctt
     1501 tctgttgtac cgatcgttaa atatgccttt aatgataaaa cagaatcaac atccctttta
     1561 aacaatgatc tcagtattga tcaaatcaca tggaatccaa ctgatttcaa agcgatgaat
     1621 gaaaagggag ttgaagtaac tgcgcctact gaaattggaa cctatacgtc ttggcaattg
     1681 acaaaaagcg gactagctaa actagctgcc agaacaaact atttatttac tatcgtgcag
     1741 acatctgatg tctatacgat taagcaaatc agcggtgcgg tagagctcag tggcagtaag
     1801 acatatgatg gaaaagcagg tgtcccgtca atccatgtca agctagcaga gggtgtcact
     1861 agttcgctta ctccagtaca agtggcactt agcagtatgg attacacagc agatgcacaa
     1921 tcagcaaaaa atatggttaa tgctggcagc tatacaatta aactaacgaa tagcggaata
     1981 gacaaagtca aagcagcaaa tcccaagttg tcatttactg atatagccaa cacatcagga
     2041 acttatacca tctataaagc taacgcagtg attactgtcg atgacgctgc ctttaattat
     2101 gatgcccaaa gtcactcgat ttcggttgga aatctccacg ttaccggtgt tgtcttaggc
     2161 gaatcattgg attataccct aacagttaac agccggacag atattggatc acaaatagta
     2221 acgattagtt taggcgaagg aagtgtaaac aataattata cgattatcgc aacagatacc
     2281 gctaagttga cgatcaatcc gactctcttg cttccttcga caggtgggat aggtctactg
     2341 ccctttgtct ttttaggact gatctttatt ttttctggct tcttttattt tatccatcgt
     2401 aagaaaaaag caggtgaaca gcgatgaaaa aaacaaaaaa tgtacgtgtg gatatatttg
     2461 ctgttctctt ttttatccta ctggtctttt ttataggttc tccgactgtg tcagctagag
     2521 aatcacctat tacatttact ttgcataatc gaatgttcga aagcgataag atgccaagta
     2581 tcagtcaaaa cgacggaata aatatcccgg ataactttca aggagtaaaa gggttgaacg
     2641 gcgttacttt cgctatatat gatgtctcgg atgaatttta caagctgcgg tcagaaggaa
     2701 gtagtgtgga agacgcacaa agaaaactag cacaaaaaag tgatagtgaa aaaatactgg
     2761 cagaaaatgt gacaaaaaca gtagacgagg aagagggcat tgcttctttt agtgcatcgg
     2821 acaaagatga gcaaggtcgt gacgctgttt accgatttgc ggaaataaag acttcagatt
     2881 aagtaaaaga aaaatcagca ccatttgtcg ttgtgcttcc tgtgacagat tcatctaatc
     2941 ataaattgac gaccatccac ttgtatccaa agagcgaaca aaaaatacct gctgcactca
     3001 cattaacaaa aacagtagag aataagcaga cagattttgc agatggggat aaagttcctt
     3061 atctcatcac cgtaacaatt cctgagaaca tcaacgagat aggaacctat acgatcaaag
     3121 atacagctga tcctcagtta tggctagaac cagaaacgat cgattttttg attggtggag
     3181 acaagattca tacatttcac acacaaaaaa caaaacatgg ttttgtctta tcagattctg
     3241 gaaaagatct tagttcgttt gctgggaaaa aattaacgat ccgttaccaa atgacattga
     3301 aagaaaacag tgaaaaaacg acatttgata acgatgtacg tttgacgact gacaatcaaa
     3361 cagtagaaac acatcttgcc atccaaacag gaggcaaaca atttgtcaaa gtggatcggc
     3421 aaacgaaaca aaaactagca gatgctcaat ttctagtcaa aaaaaatgac caatacttag
     3481 cgcaagaaaa cgggataaat cattgggtag ccaaagatga ttcaagagca acgattttta
     3541 cttccagtaa agatggaaca ttttctgtcc atggattgtc ttttggaagt tacgaactag
     3601 tggagattaa accgcctaaa ggatatatat tgagtcaatc aaccattctc tttgaagttg
     3661 aaaaaggcag ttatgatccg cttcatccta taccattgga aatcatcaac gaaccaaaag
     3721 caaatccgac gaataatgga aaaacgccag ttcagatgca gacaaatggt ccatcaaatg
     3781 aaaaaaatgg aggaagcttt ccaaaaacaa gcgaagagat gcttggaggt ttctcaattt
     3841 tagggttgtt gctagtcctt tcaacaggga cagcttggtt ttataaaaaa caacaaaaag
     3901 gaaacaatag gagggaaata aaatgaaaaa catcaaaaca aaaatatgga cgtttttagg
     3961 aactgctatc atgctgcttc ctttcgttct cggcctagga actgcagagg tttctaggcc
     4021 gtatcaccta caccagaaaa tgttacggtt aatttgcata aattgaaatt cacttcagca
     4081 ccagaaaatc aaataaacaa cggaacggaa ttgacatttc cgaatagcga accgttgaac
     4141 ggcgttgaat tcaacgtata tgacatcact gctacgtact atccaagtaa agacacagca
     4201 gttccagcag acgctacacc cttcgcaagt gtaacgactt ctggtgaagg attagctaac
     4261 ctgactcttc caggtaaatc tgatgggaaa gatgctgtat acgtctttgt agaaacacca
     4321 aagcccggtg tcgaaacgtc gccaaatatc gtgttaagct taccctttta taacccgaat
     4381 tcatcagacc ctgacaaacc tttagacacg attcacctat atgctaaaaa tgtcatcaaa
     4441 gaaagcaata tcaaaatcca gaaagctggg aacttttcag gggtagataa gttagcaggg
     4501 gcgcaattcc agatcaaaaa ttcagagaat caattcgtga ccggttttga tgaaaacact
     4561 ggtgtcgctc tctatgatcc tgctgagtca gcgttgacat tcacaactga tactgaagga
     4621 tccatcagtg tcaatggatt aatagacggc aattatactt tagttgaaac aaaagcacca
     4681 gatggttatc aattaaccaa tccacaaaaa gaaactactt ttatagtaaa aaacggaaaa
     4741 ttgcaacaaa caaccggtat catccatggc gcagatgaag ccaatcttgg gaatccaaac
     4801 ggtgtagtca atcgggaaaa tacaactacg gataacacga ttactgtaga aaatgtccaa
     4861 aactacggtg atttccactt catcaaacaa gatgtgaaca cgcaagaaaa attagccgat
     4921 gcagaatttt ctattctaag agcaaaagat ccaactgtta aattaatgaa aaaaatagca
     4981 aatcttgcct caggagaata cccatatgct tggtcagatg aagtcacagg ttctgactgg
     5041 gaagaagtga aattgatttc tgattcagaa gggaaatttt caatcgaagg gctgaaacaa
     5101 ggggcatact gccttcaaga aacaaaagca ccagaaggct acgcattacc taagaatgaa
     5161 gctgcgtaca ctgaatttac agtagatgat gatccaacaa cttctgatga aacaacgatc
     5221 gataaccaac ctaaaggaat cttgccttca actggtggta tggggatcat cgtattcatc
     5281 ttggtaggta ctgctttagt tggaggagca gtgatctact tcaaaaaacg ccatcaagaa
     5341 acagaagctt aaactattgg aaacaaaaat catacgataa ataaacgata gtgattcaaa
     5401 tggggagaaa ggttgtgaca agtggtcggt ctcttggtat tcgctcaaca aacggcaaaa
     5461 taggaggaac tattttgccg tttgttgagc gaatactcag gtcaaggcgc tgatttcaca
     5521 acctcttcat atacatatta gaaaataggt ggacatgatg aataaaagca gaataagaat
     5581 actggacatc ttcatggcaa tcattttagt cgttgggatc gggatctttt cttatccttt
     5641 tgtagaagat tcactgaatg attttcttgc ccagcaaatg atcatccatt atcaaaagca
     5701 agcatcgaat aagaattcag aggaaataaa aaaacagcag gaaaaaatga caaagaaaaa
     5761 ccaacagttg gcagagcaaa acgtatcacc agggataggc agtttcaacc aagcagttga
     5821 tgcaaaagca ttaaaagatt tacctagtaa tgcctttttt atggaacata tgcttggagt
     5881 catcgaaatc ccaaaaatca atgtcagctt acctatattc gatcagacaa cggaaatatt
     5941 tttgcaaaaa ggaacgtcat tattggaaga aagttcttat ccgactggcg ggaaaaacac
     6001 ccatgccgtt ctttctgggc atcgtggact cccagaagca aaattattta cagatttgcc
     6061 taagttgaaa aaaggagacc agtttttcat ccagatcaat ggaaagacat tggcgtatca
     6121 agtagaaaaa atccaagtgg tcttacctga tgaagtagat tctttgggta tccaaaaagg
     6181 cagagatctc gtgactttgc ttacctgtac tccttatatg gtcaatacac atcgactgct
     6241 tgtgacgggg catcgtattc cttatcaagc aaaagaggca aaaaaagcga tccaaggaat
     6301 tgatcaatgg aaaaaatgga aattttttat ttggtttatc ggtatactac ttggatcaat
     6361 cggcttagtt tggctactca tcgcttattt agattctttg gccatcgcta agcgaaacta
     6421 tcctttatct ttttatgtaa agaacacaaa cggacgacca atcgaaggaa tggtcttttc
     6481 tgtaaaaacg ctcaacggca aacactatat tactagagaa aaagttccat ttgtgaaagc
     6541 ttctgatgaa tacggactag tgagattttc tgatttaaaa ggcaggaact atcgcttgca
     6601 acatgaggag ctcctgctta aaatccatgt aaaacataaa cattcgaaac agttttcaat
     6661 gaaattgaaa aaaggaagat ataaactgcg aaaagaaaaa gaggtgtact atttgattga
     6721 gaaagaatga atgaagatgt ccaaaaaagc agagggaaca gactaaatga aaaaagagag
     6781 aagctgacga aatggacgtg taaactgcta tttttggttt actatctgga acgccagctt
     6841 ctattttttt atgtttcaga agaattttat tattatagta taacttttga attagtttcc
     6901 ataacatttg gagcaagggg ttaaaccacg agcttgagct tcgcttaaag tggcaggggt
     6961 atacgtacca tttccacatt tatgtgtatg gtattttgaa ccggtcggtg tgacatagac
     7021 agtttgttca ttattttgct caggagctgc ggcttgtgcc tgctgagagg cctgttcagc
     7081 tgctgcttgc tgtcgcgcct gttctgcttg ttgctgtgcg attgcttctt gttgcgcttg
     7141 ttctgcttgt tgtcgtgcaa tttcttcttg ctttttccgt tcttcttctg cttcttttgc
     7201 tttgattgtg gaatcaattg cagcaagacg gcttgtcaac tcttgtttgt tttcaggtag
     7261 cgcttggata gctgctgaag cagttgcata gttttctttt gtaggatttg cttcagcttg
     7321 tgacaaagca gattccgctt ctgtcacttt tttttctagt gcagcttttt ccgctttttg
     7381 tttctcttct tcttttttct tttcaatttg ttcttgttct ttcgatgaat ctgccgtact
     7441 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
     7501 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaatg caactaattt
     7561 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
     7621 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
     7681 tcccgttttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
     7741 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
     7801 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
     7861 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
     7921 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
     7981 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
     8041 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaaaaa
     8101 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
     8161 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
     8221 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
     8281 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
     8341 acggacgtca aagaatagta gccgccagat agaaggaaat tagcagttga aaaagctgcc
     8401 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
     8461 gtcttattac cgcagatgca agcttttgcc acacctcttt cttgtcattt acagactcaa
     8521 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
     8581 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
     8641 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataat
     8701 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
     8761 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
     8821 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
     8881 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
     8941 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
     9001 attttgcata attgaaggca gccaattcat taacccaaaa tagccggcaa tttggacagt
     9061 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
     9121 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
     9181 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
     9241 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
     9301 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
     9361 caaggacata cacgccccgt attctcctcc cgctcctaca cctgcgataa agcgaaagag
     9421 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
     9481 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
     9541 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
     9601 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
     9661 ctctaaagca attcccgccg atgtcgataa taataacttt cgctgatacg ttgtcatttt
     9721 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
     9781 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
     9841 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
     9901 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
     9961 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    10021 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    10081 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    10141 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    10201 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    10261 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    10321 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    10381 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    10441 caactgaagg agttagaaga ggaactagga acagaactgt ttgtacgcga aaaccgcaaa
    10501 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    10561 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    10621 tacgatcggc tgtgtggaag ctgataattc gaatacattg gcactattac tagaaaagtt
    10681 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    10741 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    10801 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    10861 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    10921 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    10981 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    11041 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catctttcaa agcgagtgac
    11101 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    11161 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    11221 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    11281 ttgtattttt tcatttttaa atcaaaaaaa ataaagagat ttacataact tgttaaataa
    11341 gcgcttacaa acataagaag aacggaaatg aatcagctat aataaccgtt gttctggatt
    11401 gtaatcatta caaagaaatg aagattataa ctttggaggt gttcgaaatg aaagaaaaaa
    11461 agcaaaaatt cgttcaaaaa acatgccctt tattcaatgg ttgggtatga ttctgctgat
    11521 tgtattagct ttattgacga tcattccttt tttatggatg gtttctgctt cattcaaagc
    11581 aaataatgaa gtgttcacga ttccgattca atggatccct aaaacttggc atttagagaa
    11641 ttatctggtt atttggtctc gtattccttt agtgacattt tttaagaata cagctttttt
    11701 aagcgttgtg atcacgttga ttcagttgtt tacttctagt tttgctgctt acgggttttc
    11761 taaactaaat tttaaagggc gcgatatttt atttttagca tatatcggca cgattgctgt
    11821 cccttggcag tcttatatga tcccgcagtt cattatgatg agaaaagtgg gtttaacata
    11881 tacgttgtgg tcgttggtgc tattacaagc gttcaatgct tttggtgtgt tcttgttgaa
    11941 gcaatactac agtagtatcc cagattcttt atgtgagagt gcgcggatcg atggcttatc
    12001 agagtgggga atctatcgca agatcatttt acctcttaca aaaccggcga ttgccagttt
    12061 gacgatcatc acattcgtta atacatggaa cgattatatg gggccattca tttatttgtc
    12121 ttctacggaa aataaaacaa tccagttagg gttgaaaatg tttattgggc tatttgatgc
    12181 agagtacgca ttaatcatgg ctgcctcagt tgtttctgtt cttcctgttg cttttatatt
    12241 tttagcgatg caaaaatatt ttgtagaagg gatcgcttca tcaggaatga aggaatagga
    12301 ggtgattatg aaagagcagc gttttgacaa aaatgtttat atggaattct tttattggtt
    12361 gtatcaatca ttggcaagta gcctttgttt ttggtgtgtc aatatcttgt atatagcagc
    12421 tgcgttcctt ttgtcgatag atggaaggaa tgtcttttcc tttatttttt ctttactttt
    12481 tattggtcca ggttgcatcg tgatgatagg tatgacagaa gaattagcaa aagaaaaaag
    12541 tttttcacca gtacgtttgt tcataaagac gtttcgccaa ttttggctaa agggattttt
    12601 ttattggcta tttgcttgga tagttagtgt gataatgatt tttgattgtt tcttttttat
    12661 acgtttttct tatggaaaat ggttgattcc gttgtttgtt ttacttgctt gtttatcagt
    12721 ttcctttagc atcaactact ggtattttca ggtgagaaat ccagcgtcga aaccaaatca
    12781 agttctacgg atagcgttct actatacgtt gaaaaaatgg tatgtgagtt tactagattt
    12841 tttactattg acaagtttgt ttctcttctt tttcgtaaag ccccaatggt gtattctact
    12901 aggtccttct atcgtatttg gtttgattta cttcaacaat cgtaaattga tgagaactat
    12961 ggatctgtag actttataaa ttgaaacttt caattaagaa tgacagtttt tatgtaaaaa
    13021 ctgtcattct ttttcatttt ttgaaacata tggctaaaca aatgaaataa aacgtgctat
    13081 acttttgaat gaaacaggtt tagaaactgt atcaattatt tgtgattatt tctaactaat
    13141 aattaaaaat aaagcataaa aaggacgatg ttcaatggac gaaaaaatga aacaaggttc
    13201 gaaatctgta gtagaaagtt tgattaatca tcatgttgat tatgtattcg gtataccagg
    13261 tgcaaaaatc gatggtgtat ttaatgaatt agaggatcaa ggtcctgaat taatcgtgac
    13321 tcgtcacgaa caaaatgctg catttatggc acaagcaatc ggacgtatta caggagagcc
    13381 aggagtagtt atcgcaacaa gtggtcctgg tgctagtaac ttagctacag gtttggtaac
    13441 agcgacagca gaaggtgacc cagttttagc aattgccgga caagtaaaac gaagcgatct
    13501 attgaagttg acccatcaaa gcatggataa tgcagcactg ttccagccaa tcacaaaata
    13561 cagtgcagaa atccaagatc cagaaacaat ttcagaagtg attgccaatg cttatcgtat
    13621 ggctaagagt tcaaaaaaag gcgcgagctt tatcagtatc ccgcaagatg tagtggatgc
    13681 acctgtccaa gggaatgtca tcaagccatt gagcgatcct aaattaggtt ctgcttctgc
    13741 agatgatatc cgttacctag ctgaacgcat tagagaagca aaactaccgg tactattagt
    13801 aggtatgcga ggatcaagtg aaaaagaaac gttagccatc aggcaattag ttggaaagac
    13861 agcattgcct gttgtagaga cattccaagc agctggagtg atctcacgtg aattagaagc
    13921 gcatttcttt ggtcgtgtgg gtttatttag aaaccaacca ggagatatgc ttttgaaacg
    13981 aagtgatcta gttattgcta ttggctatga tccaatcgaa tatgaagcaa gaaactggaa
    14041 tgcggaaaaa gatgcgcgga tcattgtgat cgatgaagcg ccagctgaaa tcgatccttt
    14101 tatgcagcct gaaagagaat tgatcggtga tatctcagcc actcttgatt tattgacagg
    14161 ttcattagag cctcaacaag tgtcagaaga tgcaaaagaa tatttagcat ctcttcaagc
    14221 agaattaacg gagcgggaca ttgttcaatc aaaaggagaa gcaggtatct tgcatccttt
    14281 agaagttatt aacacgcttc agtcaaaagt aacagatgat atgactgtta ccgtagatgt
    14341 aggtagtcat tatatttgga tggcacgcca ctttagaagt tatgaacctc gtcatttgtt
    14401 attcagtaat ggtatgcaaa cgcttggtgt tgcattgcca tgggcgattt ctgctgcatt
    14461 ggtgcgtcca aatacacaaa tcgtatcagt ttccggcgat gggggatttt tattctctgc
    14521 ccaggattta gaaacggcgg tacgtaaaaa attgaatatc gttcatttga tttggaatga
    14581 tggacactac aatatggtag agttccaaga aaaaatgaaa tatcagcggg cgtcaggtgt
    14641 agactttggt ccagttgatt ttgtgaaata tgcagaagct tttggcgcta aaggaatccg
    14701 agctacaagt gtagaggaat tagaaaaagc tttagaagaa ggatttgcga cagaaggacc
    14761 cgtaattatt gatattccga ttgattaccg tgataatgag aaattgggag aaacaatctt
    14821 accagatcaa ttctattaag gaagtgtcga catgactgaa aaaatcttat accagcacgg
    14881 gacattagga gctttgatgg ctggcttgat ggatggaaca gaaacgattg ctcatatatt
    14941 agaaaaagga actctgggat tgggaacatt gcatgggctt gatggtgaag tcatcttttt
    15001 agatggcgta gcttatcaag gaagatcaga tggctcagtt gcccaattag atggaagtga
    15061 acttaccccc tatgcagcaa ttacagattt tacgccagat actagttttt ctgtctcaaa
    15121 aactgcagat ggagaacaat tgaaaaaaga catattagta agagaagccg gggaaaacct
    15181 atttttggca gtgaagataa ctggattatt caaaaatatg cacatccgta tcatgccgaa
    15241 gcaagaaaaa ccttacagaa gactagcaaa aatttcagag tctcaaccag aattccaaca
    15301 aagcaatgtc caaggaacat tagtcgggtt ctttacgcct gaattattcc aaggagttgc
    15361 tgcagcggga tttcacttgc attttatcga tgatacacat acatttggcg gacatgtaat
    15421 ggattttgaa gtggctgaag gaaaagttga gattagccgc atctcgtccc ttgtacagca
    15481 ttttccagtt gatgatccga cttttgttca cgaaacgatt gattatgctg atttagcagc
    15541 agaaatccaa caagcagaat agaaaaagac agcgagatgc gttcaaggtg attaacctga
    15601 cgtatctcgt ttttttgagg aggaaaccta gcgtttacaa aaaaactgag ctataatctt
    15661 ctccatagat caaaaaggag cggtttttat catgtggaca gaaaaacaga aaaaagatgc
    15721 atacgaaatt ttattattac agcaacaagg attattagaa gcagaagata actggttagc
    15781 aaatttagca aattcatctg cattgttgaa tgagactttg cctgagactg tctttgcagg
    15841 atattattta ttcgatggta atgaattgat tttaggaccg ttccaagggc gagtttcttg
    15901 cacacgtatc aagatgggaa aaggagtttg tggagaatca gctgaaaaac aagaaacatt
    15961 gatcgttgat gatgtcaaaa cccatgcaaa ttacatttcc tgcgattcag ccgcacaatc
    16021 agaaatcgta gtgccaatgg taaaagaagg taatttaatc ggtgttttag atatcgattg
    16081 cggagtaacg aaagggtatg atgaaatcga tcaagcattt ctagaaaaat atgtagatct
    16141 tttattagca aattttaata taaatgcttt ttcatttatt aataactaaa atcgtctatt
    16201 gaaacaaaaa agaagagaaa tcaagaataa aaagagccgg aaagacagtg ggcatttctg
    16261 tttttcggtt tttttatttt tttataaaat cacaagtgtt attgattttc ataatcatat
    16321 tttaatggta ttatcatttt aaataatgga aaaacattaa ggatggatga aatatgaata
    16381 acgaaactat cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag
    16441 tggttggaga agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc
    16501 atgacgtcga tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc
    16561 acgtttatga gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata
    16621 ctttagtttc ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga
    16681 aggcacttta tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc
    16741 ctagagaatc tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc
    16801 cagaactgtc ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa
    16861 tcatttcaaa aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa
    16921 gcatttgtaa aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg
    16981 aagaagatga agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt
    17041 ctttaccctg ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt
    17101 tttctcattt gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat
    17161 gttctttgat gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa
    17221 cagaattctt ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc
    17281 agtattaggc aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt
    17341 aatacatata tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt
    17401 attaacgatt atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg
    17461 tatgatatta tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DACCDF010000055        17081 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCDF010000055 DACCDF010000000
VERSION     DACCDF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.058"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(17..538)
                     /locus_tag="IVK51_001891"
     CDS             complement(17..538)
                     /locus_tag="IVK51_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP6431472.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="IVK51_001892"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="IVK51_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP6431473.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="IVK51_001893"
     CDS             4618..5505
                     /locus_tag="IVK51_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP6431474.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="IVK51_001894"
     CDS             5502..6671
                     /locus_tag="IVK51_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431475.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="IVK51_001895"
     CDS             6695..8050
                     /locus_tag="IVK51_001895"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP6431476.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IVK51_001896"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="IVK51_001896"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP6431477.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="IVK51_001897"
     CDS             10336..10626
                     /locus_tag="IVK51_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431478.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="IVK51_001898"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="IVK51_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP6431479.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="IVK51_001899"
     CDS             11667..12326
                     /locus_tag="IVK51_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP6431480.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="IVK51_001900"
     CDS             12483..13415
                     /locus_tag="IVK51_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431481.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="IVK51_001901"
     CDS             13569..14858
                     /locus_tag="IVK51_001901"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP6431482.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="IVK51_001902"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="IVK51_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP6431483.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="IVK51_001903"
     CDS             16438..17025
                     /locus_tag="IVK51_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP6431484.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
BASE COUNT         5587 a         3065 c         3740 g         4689 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtattgaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag t
//
LOCUS       DACCDF010000056        17045 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCDF010000056 DACCDF010000000
VERSION     DACCDF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.059"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(103..1884)
                     /locus_tag="IVK51_001904"
     CDS             complement(103..1884)
                     /locus_tag="IVK51_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6431485.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(1910..2824)
                     /locus_tag="IVK51_001905"
     CDS             complement(1910..2824)
                     /locus_tag="IVK51_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431486.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(2851..3813)
                     /locus_tag="IVK51_001906"
     CDS             complement(2851..3813)
                     /locus_tag="IVK51_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431487.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(3813..4757)
                     /locus_tag="IVK51_001907"
     CDS             complement(3813..4757)
                     /locus_tag="IVK51_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431488.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(4758..5762)
                     /locus_tag="IVK51_001908"
     CDS             complement(4758..5762)
                     /locus_tag="IVK51_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431489.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(6030..6272)
                     /gene="acpP"
                     /locus_tag="IVK51_001909"
     CDS             complement(6030..6272)
                     /gene="acpP"
                     /locus_tag="IVK51_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP6431490.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(6394..7395)
                     /gene="plsX"
                     /locus_tag="IVK51_001910"
     CDS             complement(6394..7395)
                     /gene="plsX"
                     /locus_tag="IVK51_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP6431491.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(7444..9480)
                     /gene="recG"
                     /locus_tag="IVK51_001911"
     CDS             complement(7444..9480)
                     /gene="recG"
                     /locus_tag="IVK51_001911"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP6431492.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(9597..11279)
                     /locus_tag="IVK51_001912"
     CDS             complement(9597..11279)
                     /locus_tag="IVK51_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP6431493.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(11331..11693)
                     /locus_tag="IVK51_001913"
     CDS             complement(11331..11693)
                     /locus_tag="IVK51_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP6431494.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            11963..12151
                     /locus_tag="IVK51_001914"
     CDS             11963..12151
                     /locus_tag="IVK51_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP6431495.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            12389..15058
                     /locus_tag="IVK51_001915"
     CDS             12389..15058
                     /locus_tag="IVK51_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6431496.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(15149..15769)
                     /locus_tag="IVK51_001916"
     CDS             complement(15149..15769)
                     /locus_tag="IVK51_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6431497.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            15881..16282
                     /locus_tag="IVK51_001917"
     CDS             15881..16282
                     /locus_tag="IVK51_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431498.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANS"
     gene            16417..16755
                     /locus_tag="IVK51_001918"
     CDS             16417..16755
                     /locus_tag="IVK51_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP6431499.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         4809 a         3539 c         3080 g         5617 t
ORIGIN      
        1 aagtcatgaa atcagcgcct tgacctgaat attagagaaa taaacagaaa aatatcaaaa
       61 aaccgaaaaa ggacatcgcc ctttttcggt catttttagt gattatgaac gtgaatctgc
      121 tgtaacagaa acatttgccc atgggtttgg tacatcataa gaccaatccc agtctttgac
      181 acggtcgttg actggcaata cttcattacg gaacaacgtt ggaatcgcaa atgcttcttc
      241 aaatgcatat tgttgccatt tttgatatgc ttctttttgg tactcttcat caaatgcttt
      301 gcttgaactg atatcgttga tcaattttgt attttcttct gattcataac gagtgtagtt
      361 gaacgcagca tttggtcccc atagtccact tggatttgga tcagttcctg tattccaagc
      421 agcttggtaa acatcaattt caggatcatc attttctaat ttatcataga aagaattgaa
      481 ttcgatcaga cgacctgttg tgtattggac atccaaaccg atttctttcc actgttggat
      541 atagtaatct gccaatggtt gcgctgtttc cccaccggac atcgaagcaa agttgattgt
      601 tagtttattt ccatctttgt cttctcttaa accatcgtca tccacatctt tgtagccagc
      661 atcatctaat agttttttcg ctttgtctag atcttgtgtg aatcctttca tctcatcatc
      721 atggtatgac tggaatactg gaggaatcaa tgaagtcgca ttgcttctca aacctgcata
      781 gaaacgctca cctactgcat tattatctaa tgcatagccc attgcttgac gtaatgattt
      841 atcagccatt tttgaatctg gattgtattc cacactcttt gtatctgcat tccatttacc
      901 taacttgaat cctagataag tataagaagt ttgttcacgg cctaagtttg tatagccagc
      961 aatttcttta taggtatcgt aagtatctgt tggcatttgg aaaatcatat catataattt
     1021 agagttcaat gcttcagttg cacttgccat cggtacagat ttcatcgtga tctttttcaa
     1081 tttaggcgta ccattccagt agtgctcgtt tggcaagtat tctactgatt ctcctgcaac
     1141 gatattactc atgtaataag ggccaaaagt cactggattt ttacgtactt gatcgcttga
     1201 ttccatatct ttgattgcta caccttctaa cgtatgtttt ggtgctgcat atgaccaaat
     1261 accaccaccc gattgaagca tgcttgggtt tacttcttta tattcgattt caactgtttt
     1321 gtcgtctatt tttttgattc cggagattgt gtcggcactt ccatcatgat attcttccat
     1381 acctacgatg ttacggaaag tgtcgtcata acggatacca gtgtaatctt tattcccaat
     1441 gatttcgtaa gggaaaatca catcgtccgc cgttacatct tcaccatctg accatttgac
     1501 gttgtctttt actttgattg tcgctttttt cgtatcttca tctagatcaa gcgttgcagc
     1561 tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat agcggttcat gagaaggagc
     1621 cataaaagct gcatcatatg aatcttggta aaactcccac tggaacaatc ctttaaactg
     1681 actgtccgtt gcgaccgcag cttctaattc gccgtcttcg attgcatcat cattattttt
     1741 tgttgcaatt ggaaaagtta attcctcttt gttattgccg ttgcctccag agttgtttcc
     1801 agaggtatct gtacctccgc ccccgcatgc tgcaagaacg acagcagaca tcagcgctgc
     1861 aaatccaaaa atcgttttct tcattcctat ttcccccttc gcttctatac tagcctaacc
     1921 gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac ataattgata ctcaacatca
     1981 aaactaaaat aaggattgat gcgggtaacc agatccattg ccgattcact aagacttctc
     2041 cgttacttgc ataaccgatc aatgtaccta aactaggtac atttgccggc aagccgaaac
     2101 ctaagaaagt caaagttgtc tcgataccga tgtttgccgc aaagttcatc gtcaaattag
     2161 taatgattaa tgaacttaga ttcggcatga tttcgcggaa catgatttta aaatcattgg
     2221 ttcccattgt ttttgaggca ctgacataat ctcttcttgc ttcagataat gtcttgcttc
     2281 ggaataatcg ggctttagct acccaataaa atgcactcat gatgaatata aaggaccaga
     2341 cattgtatct ggaaataatc gatacgaaga cgataatgat cagcatgatc ggtagaatca
     2401 tgataaaatc aacgatccgc atcaagatgt tgtcaatgat ccctccgtag tagccagaga
     2461 tgatccctaa tccaaccccg atgatcgaag taaggatcgt gatcgcaaat ccgatcgtaa
     2521 cagagtttct agcacctatg atcaattgtc ctaatacatc acgtccgcct tcatctgctc
     2581 ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg tgcagcgtat ttgtcgaaga
     2641 tactaacata catcactttg tcttgatcgg tcagcatcgc accgataaag atcactaata
     2701 ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt atctttttta aattctcgag
     2761 caatcattcg tatccccata ggaggaatac tttcttgaac tgctgtattg tttttttcac
     2821 tcatcgatcc attctccttt ctgattggtt ttattgaatc cgaatacgcg ggtcaacgat
     2881 actcatgatg atatctgaaa gtaatgtccc cactagcgtc gcgattccta aaatcaatac
     2941 taaggatgtg atcacggtat agtcccgttg gccgatggaa tcaataaata gttttccgac
     3001 tcccggataa ccaaaaatct tttcgataac tacagaacca gcaatcaacg aagtgatttc
     3061 ataccccatt tgagaagcca tcggcaacgc tgcatttcgg aaaatatgtc gactgaatac
     3121 tttattcgtc ggtacccctt tggaacgggc tgttctgaca aaatcgagcg atttcgaatc
     3181 gattacttca cttcgcagat attggattgt gacggcagtc gccaaaaatg cttgggtaat
     3241 tgatggtaac agaatatatt tcaaacggtc tatccattga gtgataaatc caccttgcaa
     3301 acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa cgataaccaa aaacaaatag
     3361 catgatcaag ccgaagataa ataaaggaac agcaaaacta aagaagttgt agattacgac
     3421 aatcttatcc aagattgaat tttgataccg gccagcaagc atccccagag gcacagcaat
     3481 caaataagta aggacaactg tcaagatcga tagatataaa gtgttgatga tccgttcgcc
     3541 gatcaagtca gcaacagagc gtttgtataa gaaactttga ccaaaatcgc cttgcaaagc
     3601 atttccgatc caacggaagt actggacata ccatgggtca ttcaagccag ctgcttgtcg
     3661 catctgttcg atcactgccg gatcctgatt tggattgatc aaaccggtaa atggatcccc
     3721 cggcatcgct tgggctaata agaagatcag aacacttaaa ataataactt gcgggatcat
     3781 caataaaaca cgtcgcagaa tcgttttcca cattagcttg cacctccatc tttcaacgcc
     3841 actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc gaccagtcgc atcatagtat
     3901 tctttttggt tttggatata ctcttgttct actcttcgtc gattttcttt gtggctttcc
     3961 ctattttcta catcgatttg cggaatagct gaaagcagac gtttcgtata gatatgtcgc
     4021 gggtcattat aaatgtcttc tcttgtaccg atttctacaa atcgaccttt gtacataatt
     4081 gcgatattgt cgcacatgtg ttttactaca cctaaatcat gtgaaatgaa taaatagctg
     4141 aggccgtatt cttgctggat tcgtttcatg aaattcaata cttgcgcttg aacagataga
     4201 tccagagcag atacaggttc atcggctacg atcagttttg gattggtagc aacagcacgg
     4261 gctactccta gacgttgacg ttgtccgcct gaaaattcat gaggatattt atatagcgca
     4321 tcttccggca tcccaacgat atctagcagt ccttttacct ttttcttttc ttcttgatcg
     4381 cttaaccgtt caaaatttcg gatgggttca gcaatgatat ccaacactcg ctttttagga
     4441 ttcaaactgg acattgaatc ttggaagatc atctgcacat ccttgttata acccattttt
     4501 ttgcggttgc ttcgcttcgt tacatctttt ccttcataca tgatttgtcc ggctgtcacc
     4561 ttttctaatc ccacaacggc ttttcccgta gtagattttc cagagccaga ttctcccact
     4621 aaaccatatg tctttccttt ttcgataata aaatccacac catctacagc atatacatga
     4681 tccgttactc gattgaagaa tccactgcga atagggtaat gaactttcag gtctttgatt
     4741 gtgattaatt ctgtcatcta cacctctcct ttttcgtctc taaagtggaa atgcttatag
     4801 caggagcaac gtaccaagtg gttcggtgcg acttcatgaa ggataggctc ttcttcatga
     4861 tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac aacctttacg aggcatgttt
     4921 ttcagagaag gaacgacacc ttcgatcaca tggagttctg catcatgatt tccttcttgt
     4981 ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag gatgatcaaa cagttctttt
     5041 acacctgcta cttcaacgaa ctgtccgcca tacatcactg ccactcgatc agccatttct
     5101 gctacgactc ctaagtcatg agtgatcaag atgatccctg tttttgtttc ttcctgaagg
     5161 tctttcagta aatcaagaat ctgtgcttgg atggttacat ctagggctgt cgtcggttca
     5221 tctgcaatga cgattggagg tttgcaggcg attgcgattg cgatgatgac acgttgtctc
     5281 ataccgcctg atagttcatg gggatattgg cgtccaactc tcgcaggatt aggaatccct
     5341 acttgttcta gcaattctag tgcacgagct tgacgctgtt ctgcattcat ctttgtgtga
     5401 taggaaagtc cttctttgat ctggtcttcg attctcatga gaggatttaa tgcagacaac
     5461 ggatcttgga aaatcattcc gatatcattt ccgcggattt tattatagag cgtttcgttc
     5521 aggtcgatta aattcagatc gttatataaa atctcacctg taatttttgt gttattagga
     5581 tcaagtaatc ccataatcgt agtagccaag gtacttttcc cgcacccaga ttccccaacg
     5641 attgctaaaa tctcgttttt gtctaaagta aatgaaacgt catctactgc atcgtaatag
     5701 tcatctttga gtcggaatcc tgtatgtagg ttctgaactt ctaataattg gttatcggac
     5761 aatcgaatac ccctctctgc ttcctaaatg gaagtttttc ttaataaaaa aggacattac
     5821 acagaataaa gcgttttata acaaaggtta ttcttatctt tgtgtaatga attattaata
     5881 attatataga aaatcgcttt actttgcaac ataatttctg tttatcgcca gaattatcag
     5941 aaaaatgact tttacgtgtc aaaaagatta aaaaaagaag gataaaaagc tgtatgcagc
     6001 tttttatcct tctttgcttt tatttgaaat tatttaagat ggctgctgat ataatctact
     6061 gcgccaccga ctgtttcaat ttgttcagcg tcttcatctg aaatttccgt accgaattca
     6121 tcttcaagct ctaaaacgaa ttccataata ctaattgaat cagcttttaa atcatctttg
     6181 atgttcaatt catccgtcac tttatcggta tctatctcaa aatgatttga aataattttc
     6241 gctactttgt taaatacttc ttcgcgagtc aactttttca cctccaagaa tcatacactt
     6301 gtcaattacc tacaccatgg ctaattttac tggcattttt caggaatgtc cacctttttt
     6361 tcaatggaac ggattgcttc agatatccaa atactattct tcgctttttt caaattgttc
     6421 gactaattgt ccaactacat ctgtctcaag cattgtgtgg atttggcgga tcgtatagcg
     6481 aacagcatcg gggcctgttg ctccatgagt tttaatgact ggtgctttca agccaaacaa
     6541 aacggcaccg ccatgtttgg aataatccat ttctgctttc aaagaacgta agccgtcttt
     6601 taaaagcata gcgcccattt ttcctttgat cccttcattt agaatagcag attttaatag
     6661 attcatcata ttcattgctg ttccttcaat agatttcaaa acagcgttgc ctgtaaatcc
     6721 atctgtgacc actacatcag ccacaccatt gagtagctca cgagcttcta cgtttccaat
     6781 aaaattcaag cttgtttcat tctgaaggag ttcataggct tttttcgtca cttcactacc
     6841 ttttgttgct tctgttccgt tgtttaagag tgcgacacga ggacgtccga tacctcggac
     6901 tttacgtgca taaaaagaac ctaaaatacc gtattgcaac aaatgttctg gtttgttttc
     6961 tgcgttagcg ccaagatcca gcatatcgaa accggcacct tctttcccta ctactggcaa
     7021 ggtagacatc aagccagggc gttcgatatt tttgatacgt cctacgatga acaaccctgc
     7081 tgctagtagc gcgcctgtat tccctgctga aaatacagca tctgcttcac cgtttttcac
     7141 tgcttgtgca gcaaggacca ttgatgcagt cttttttcgt cgaattgctt ttactggctc
     7201 atcatcacta ttgatttttt cgtctgtatg gataatcgtg atgttttttt catttgttaa
     7261 gtatttcttg atttctgctt cttttccgta aagtaagaat tctacttctg gaaaatcttg
     7321 ctgcgctaaa gccacgcctt ctacaattgc ttgaggtgcg tgatctccgc ccattgcatc
     7381 tacggcaatt ttcattgttt ccactccttt ttccgtcatc agtataaagt atagcacagt
     7441 ctgctaatcg aaataacgat tatcctgatt ttctatttgg aggctttgcg ctaaaggctg
     7501 atactcagga agcatctgcc aatcctttat ctgccaaagg gctgcagcct cttctctggc
     7561 tacctccaaa acatttccat ccgtcaccaa gtcagcaatc gcaaattgag gcagaccaga
     7621 ttgacggaag ccaaatactt caccaggacc acgtaattcc agatcttttt cgcttagtac
     7681 aaaaccattg gtggtctctg tcatgatttt catccgttcc acacctagtt catttttagg
     7741 attagcaact aagatacagt aggaagcatc acttccgcgt ccaacacgtc cacgaagttg
     7801 atgcagctgt gccaatccaa atcgatccgc atccatgatc agcatgacgg tcgcgttcgg
     7861 cacgtttacc cctacttcaa ttacagtagt cgaaactaat atctgcatct ggttgtcttt
     7921 gaaggcttcc atgattactt ctttttcctg attcttcatt ttaccatgta acaagccaac
     7981 ttcatatttt ggtgcaaata gttcacgaag tttttcatag atttccactg cattttttac
     8041 atctaatgct tctgattctt caatcagcgg acagatgaca tacatttgat gtcctcttgc
     8101 cagttctttt ttcgtccaat ccagtacact gtccaattga ggtgttctta cccagcgtgt
     8161 ttcgatcggg atacgtccag ctggaagttc atcaatgata gatacatcca tctcaccata
     8221 tgctgtaata gctaatgttc taggaatcgg agtcgctgtc ataaataaga catctggtcg
     8281 ccagcctttt tcacgaagta ctttccgctg gttcacgcca aaacggtgct gctcatccgt
     8341 aataactaga cctaaattct gaaattcgac tccatcttgg atcaatgcat gtgtgccgat
     8401 cacgatgtta atttcccctt gctctagttt ttcaaggagc tcacgacgtt cttttgcttt
     8461 cgttgacccc gtaagcaatg cgatggttac ttcattggga tcaaacagct gttgcaagct
     8521 ttccatatgt tgttgtgcca agatttctgt aggcaccatc aacgcacctt ggaaacctgc
     8581 tgtaacggta gcgtataacg caatcgcggc aaccactgtt tttccgctac ctacatctcc
     8641 ctgcaaaagt cgctgcatat gctgcggact gcgaagatcc cgacagattt cattcgttac
     8701 acgtttttgt gcatttgtca gttcaaaagg tagcttttga gtaaattctt tcaatttttg
     8761 gacatcataa aggatctcca aaccattcgt ttctgctttt tctgcttttt tcaatccttg
     8821 aagacgcatt tgaaataaaa agaactcttc aaaaacgaca cgtcgtttgg cttgatggtt
     8881 ttcttcgtgg tcttttggaa aatgcatact tcttacagca gaagctcggt caagcagtcg
     8941 atatttctcc acaaggttgt ttggcagatt ttcttcaatc aatgtgccgt attcttgaaa
     9001 cccttttttg attagatcaa ctaatgtcgt ttgacgaatt gatttgttga cgtgatatat
     9061 cggcgaaaag tcatccattg tttgagaacc taaaatcttc atcccgttca atgctttacg
     9121 ctttgcatcc catttaccgt atacggcgat ttcctccgat aaaatgactt tttcttttag
     9181 ataaggttga ttaaaaaaag aaacgttgaa cacatcatgt tcttgcatca tacgaaattg
     9241 caatcggctt tttttataac caaatcgatt catcacagga ggcgagacta cgattccttt
     9301 caaagtaacc ttttcttgat cttggatctc gtttagattt ctttcctgaa tatcttcgta
     9361 gcggaaagga taataagtca gtaagtcttc aatcgtttgg atgcccaagc tggcaagact
     9421 agctgctctt ttttctccga ctcctgccag catagtgatt ggatcatcta aagaacgcat
     9481 aaatttttcc tccctccctt aacaaaaaaa ggacttggtc agcgatcata cctgatcact
     9541 aaccgaaggc cttttatgaa tgaatactgt gttagaaaga tattctgaca cagaaattat
     9601 tcagcagaaa atagataagg atagacaggc tgatctcctt ggtggatctc gatttccaaa
     9661 tcaggatgtt tttctgttag tttctcagca aattcatttg cttgttcaac tgttccttca
     9721 tcacctgcca agatcgttac gatctctgtg tcctcattga tcatttgttc tagtgtactt
     9781 aacgctgttg ctgtgatatc tgggttagag atcaggattt ttccatcgat catgcccaaa
     9841 aagtctcctt ctttgatctc tacaccgtca attgaggtat ctctgatcgc gtgagtgact
     9901 tgaccgctca caacgctaga caacatttct gtcatcgttt ctttgttttc ttctaaagaa
     9961 gcttgatcat tgaatgaaag catagctgtc aatccttggg aaatcgttct tgaaggaaca
    10021 actgctactg ggatatctgc tacttctgct gcttgatcag ctgccatgaa gatgttctta
    10081 ttgtttggca agacaatcac tttatctgcg tttacttctt gaatcgcttt aacaatgtct
    10141 tccgtacttg gattcattgt ttgcccacca ctgataacat aattagcgcc taaactttca
    10201 aacagctctt ggacgccttt accagcagcg atcgctacga taccatacgg gatacgtggt
    10261 gtttcttttt tcgcaaactc gttcacttct tcatcgtgtt ccaaaatcgt ctcatgctgc
    10321 aagcgcatgt tgtcgacttt gattttgact aatgaaccga atttttgtcc ataattcaag
    10381 acttctcctg ggttttctgt atgcacgtgg actttgatga tctcgtcatc attgaccaca
    10441 agtaaagaat ctcctatacc atctaaatag ttacggaatt cttcgtaatc gaatgtacta
    10501 tcgactgtag gtccttctcc gatttttacc atgatttctg tacagtaacc aaaatggata
    10561 tcttctgtcg ccatttgacc ttggacactg cgatgatgtt ctgcattgac catatcatcc
    10621 atttctgctg gagaaggttc atatgtttca tccgcttgga attctccatt caatgcactt
    10681 aaaaagccct cataaacaaa cagcagtcct tgccctccac tgtctacgac accgacttct
    10741 tttaggactg gaagcaagtc tggagtttta gctaaagcac gctttgcccc ttttacaaca
    10801 gctgtcatta cttcaacgac atcatcagta ctttttgctt tcttttctcc agctttcgct
    10861 gcttctctag ctactgtcaa aatcgttcct tctactggtt tcattactgc tttatacgca
    10921 gtttcaactc catgagtgaa ggctgcagca agatcggaag catttaatga gacaacatct
    10981 ggaatttgct tagaaaatcc acgaaacaat tgagacaaaa taacgccgga atttccgcgc
    11041 gcacccatca gcaatccttt tgaaagcatt gctgctaatt ctcctacttt ttctgaggtt
    11101 gaatctacta ctgccttagc tccactagtc atagatagat tcatatttgt tcccgtatcg
    11161 ccatctggca cagggaatac atttaatgaa ttgacgtatt ctgcatttac ttgcagacga
    11221 ttcgcacctg cctggaccat ttcctggaac tggcttgcac ttatttctgt tacactcacc
    11281 tagataatcc tccttcagat cgactgctga gttaccagcc agtctcctgc ctagtctggc
    11341 agtacacgaa caccttgtac aaagacatta actgaattgg cagttacgcc taataatgcc
    11401 tctagattgt atttcacttt ttcttggacg ttgcgggaaa cttcagagat tttcgtacca
    11461 tagctgataa tcgtataaac atctacagca ataccattat cttcttggcg gacaactact
    11521 cctcttgagt aattttcttt acgcaaaatc tcagtaatat tatctttaat ttgatttttg
    11581 ctggccattc ctacaacacc gaacacatct gtcgcggcgc caccaacaac tgttgcaatt
    11641 acatcattgg taatttcaat ggttccagct gacgtttgaa tttttacagc catgaatgag
    11701 cctccttaac gggtaaaaaa ctcccatttt tcttctatca tattttatca tagcgcaact
    11761 tgaaataaaa ggcagttacc caaagtactc aaaaaccttg caaaatttca ggtataacct
    11821 tgtttaattt acaattattc ttctcaattg tcaattgtat ctatgaaaat gtacttgcaa
    11881 atttccattc tatatgatat gttagtctgg tatgagctga attattctat tgctccaaat
    11941 caaggcaaag gaggaaatta ccatggcaaa agtttgttac tttacaggtc gcaagacaaa
    12001 aagtggaaac aaccgttcac acgctatgaa ctcaacaaaa cgtactgtta aacctaactt
    12061 acaaaaagtt cgcgttatgg tcgacggaaa acctaaaaaa gtttgggtgt caactcgtgc
    12121 tttgaaatca ggaaaagttg aacgcgttta attgaatgat acttggaccg ttcagatagt
    12181 gaacggtttt ttatttaata aaaattttta agggtgggac ttttgattcc tacccttttt
    12241 ttgcgtttat ttttgttata cgaacaaatt atgaagttat ttattctaaa ttttattttt
    12301 ttgtgaaaat tagactcttt ttaagcagag aaaaacaagg atatgataaa ctgaatacaa
    12361 cttcatagaa atttggtgga aaataaatat gaaaaaaaat ttaaagtcat tctttagaga
    12421 aaacggacgc tacgcagcag ctagtttcct gattccgttt ttgattttag cacttgttta
    12481 tctaagtatc gggatctatc ctggaagcag cagaagtatt ttggcaagtg atgccttttc
    12541 acagttttct aattttcatg ccagcttccg aaatgttctt cttggaaagc aaagtatttt
    12601 ttatacatgg aatgcttctc taggattgaa ttatttatct ttaatttctt actatctagg
    12661 cgggatcttc acaccactgg tgttcttttt tccaaaccag atgatgccag atgcactgta
    12721 tgttctgact ttgcttaagg tcggctgtgc cggattggct ttttggtttc ttgcacgtac
    12781 gttcaagatt cctcgctggg ggcatgttgc acttagcgtc agttacgcat tgatctcatt
    12841 tatcactgca cattcggaga tcatcatgtg gctggatgca tttgtttatc ttccattagt
    12901 gattttgggg attcatcgtg ttatggatct taaacggccg aaacttttat ttatcagtta
    12961 tttactcttg tttttatcca gtttttatat gggttttatg attggcgtat tttctttctt
    13021 atattttatc gcccgtctat tgacaaactg gtcagaatat aaaaaaacga ttgttcctta
    13081 tggtatcact tctttactgg caggcggcgc ttcaatgatc attgtattgc cagcagtgct
    13141 ggatcttcgg tcaaatggcg aggcactgag tcagatcact acgttgaaaa cagaagcgac
    13201 ttcttatctg gatctaatca tgaaaaatat gattggtgtt tatgacacga caaaatacgg
    13261 atcgattccg ttcgtttacg caggtctgct accattagtc ctctgtctgt tttattttgc
    13321 cagtcggaaa gtaccgttga aaaataaatt tttgtacggc agtcttttcg ccatacttat
    13381 cgcaagtttc tatcttgttc cattgaattt gttctggcat ggtatgcatg caccgaatat
    13441 gtttttattc cgttatgcct atctgttctc tttcttagtg ctcctgcttg caggaaaagg
    13501 atgggaaaag atagaacaag acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc
    13561 tgctctcttt gcggtggcat ggggcatcaa acctgaagga agctacacct atgtgacgat
    13621 gacttccttt gtattgacag tggttttctt agggatttat gcttttgctg tgggatttta
    13681 tcagcataaa aaactttcat tcaatcatct gagtatattg ttattattga tcatgtctgc
    13741 tgaagcactg gtcaatacga acagtatgat ccatggcatt cttgatgatt ggaattatgc
    13801 ttcccgcagt ttatatactg atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa
    13861 aaaagattcc gactcgtttt accgcttgga aaatctggat ccagtatctg caaacgattc
    13921 gatcaactat ggttacagtg gtatcagttt gttttcttct ataagaaatc gtaattcttc
    13981 ctcttacctg gataaattag gtttccgttc aagagggaca aatttgaata tccgttataa
    14041 caataatact ttgctaatgg atgggtttac aggaatcaaa tataacattt ctaaaaacga
    14101 aagcacgttc agtaaatatg gcttccgcca agctggtcaa agtggcgact atacactgta
    14161 tcagaatgtc aatgcattgc cactagcatt tactgcaccg ttgtccgtta atgatgttga
    14221 gcagccagtg acagataatt tgaccagtca gacaaacttg atcaatacat tgtctggttt
    14281 aaatgaacag tattttacat tctatacacc tactttgaag aatcagttga atacgacgat
    14341 cacaaagaca acgactggcg tcacttactc agaaacagca gataaccagc caaaagagct
    14401 cacttgggaa gtccatgtgc ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa
    14461 ttttgcacaa ttagaaagtt ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac
    14521 gcaaatcaac atttctggtc aatactatga tcttggctat tatcctcaag agacaacagt
    14581 gacatttact gcaagttttt atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt
    14641 tggtctagat gttgtagcct atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga
    14701 aatggaagcc actggcagaa aagcttctgg gactgtaaat accgctgaag attccatgtt
    14761 ggtgacaact attccttatg acaaaggatg gaaagctaaa attgatggga aaccagtcaa
    14821 ggtcagcaat ttcaaagacg cattcttgat gataaaagtt ccagcaggta aacatactgt
    14881 cactttcagt tacttgcctg aaggatttac gataggtgca atcctttttg tcctatgtat
    14941 tgcattgttt attctttatg tctggtacct tgaccgcaat cgcccctttt atggcacgat
    15001 tgatccagca atagaagaaa aaccgcgaaa tacacacaga agaagacgaa gaaaataaag
    15061 aaacgatgga atctgtgtca ctcttctatt taaatggcaa aaatttcaat ctttaaatga
    15121 caaaaaccac ctgatttttg gtggtttttt atatacctat gactaaacta tctcttgctg
    15181 cttcgacaac ttctacagtg atcttatcca tttgattgat tcttaaaatc cgatcaatct
    15241 gcgcaaataa acgatgaata agcctaaaat ttccttttgt gatcttaatg atagttgtga
    15301 tagcctcata atcagaaaaa tcttctagct tgatatcaat tcctaaatct gcccatttgt
    15361 attccaaaat atgatgtgtt tcgtccttac taaggttatc aaattcatgt gcaaatccta
    15421 tgcgagagta aagctgcgga taacgagaca atcttttttc aataccaggc ataccgataa
    15481 gaaccattgc cagatcattt ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct
    15541 ttaaacgatc aatttcatcg acaataatta agtcaattgc ttcataggtt ttaggcacag
    15601 gaggatcttc ttgattgtat tttagtacca tatgcattgc tttagtcaga cctattctac
    15661 tagttattgt gttaatatcc gaactgacac gagacatctt ttcagctggt acagtataaa
    15721 aaactttatt tacacttaat atttcatctg ttgcatttat caagaacaag aaaaagtctg
    15781 aatattcaaa aaataacggt gtacattttt aaaaggagat gtataatcga gatttagaaa
    15841 gcgttttcct aaaaatttaa tgaattagga agtgattact atgaaaaaat tatttagcgc
    15901 actttttatt ctgttatttg gctttattgg tattgggact tctggaaata tagttaaagc
    15961 tagcacgatg gttgaacaaa caagtgaaat ggcaaaaact tttagtcaat taacaccaga
    16021 agaacaaagt tacttcaaat ccaaaggatt tagtgataaa gatacttttt acactcaaaa
    16081 gatagagcct caagatccga aaacacgttt ttctgcaaat gtattgcggt taaccgcatc
    16141 tactaaaaaa attactaata cgcaggggcg gacagaatat attataacaa gtcccaattc
    16201 ggcgatgtat cgtatttcta cacgcttgag tttaggtagt gcaaaatcat atactcaaac
    16261 agtaaatcta gctaattctt aaggttatgg tggaggaatg tattttacat ttactgggaa
    16321 aagacaatat tacagtgtaa aattagctat gcaagtttat aatacatttg gtgtagggtc
    16381 agtctcttgc actgcgggag gattgacaat aggaaaatga taatttctta ctatgtctta
    16441 aatagaagtg atgatgaagg actcttcgtt tttcaatcat tacatgagga tacgatgcta
    16501 aaagagtttt ataatcggat taagaataaa cagatagaaa caaatttata ttttttaagt
    16561 gactctatct tgccttttat tagcaaccct aaaattctta acttgttgca gacaaaagat
    16621 ttgaaaagtt taccgattat tgtcattgat gaggaaattg taaaatatgg cgcttatcta
    16681 agcgtagaag aattagaaaa attaactaac attggaatga gtattcagga ggataatggg
    16741 gtacatggaa catagaatga atatgcacag ttgagaacgg aatttgacat ttatttagac
    16801 caacagggta taccctgttg gtctttttgt gcgttcatga tatacttggt gtcattttta
    16861 tatttaaatg gcacaaatat ttctaattat ttgtcattta acgctgaaaa tttttgctat
    16921 ttatctaaaa aagtgacagg atactcattt ggccttcata gaattttcta aatatgaatc
    16981 atcaatgaat ttaattaaaa aaataagtag ggcaaaatat aaatgttttt gccttacttg
    17041 tttcc
//
LOCUS       DACCDF010000057        16591 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCDF010000057 DACCDF010000000
VERSION     DACCDF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.060"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..779)
                     /locus_tag="IVK51_001919"
     CDS             complement(45..779)
                     /locus_tag="IVK51_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP6431500.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRFVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1105..1515
                     /locus_tag="IVK51_001920"
     CDS             1105..1515
                     /locus_tag="IVK51_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP6431501.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     EGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            1508..2209
                     /locus_tag="IVK51_001921"
     CDS             1508..2209
                     /locus_tag="IVK51_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP6431502.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2209..3822
                     /locus_tag="IVK51_001922"
     CDS             2209..3822
                     /locus_tag="IVK51_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6431503.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            3812..4033
                     /locus_tag="IVK51_001923"
     CDS             3812..4033
                     /locus_tag="IVK51_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP6431504.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4030..4791
                     /locus_tag="IVK51_001924"
     CDS             4030..4791
                     /locus_tag="IVK51_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP6431505.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPETFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTSGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRRKSGTIINMASIAGLVAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5172..5681
                     /locus_tag="IVK51_001925"
     CDS             5172..5681
                     /locus_tag="IVK51_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP6431506.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANMASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5751..6290)
                     /locus_tag="IVK51_001926"
     CDS             complement(5751..6290)
                     /locus_tag="IVK51_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP6431507.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVVVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IVK51_001927"
     CDS             complement(6430..7041)
                     /gene="rpsD"
                     /locus_tag="IVK51_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP6431508.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7357..8154
                     /locus_tag="IVK51_001928"
     CDS             7357..8154
                     /locus_tag="IVK51_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP6431509.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     QGQTDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8182..8817
                     /locus_tag="IVK51_001929"
     CDS             8182..8817
                     /locus_tag="IVK51_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP6431510.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVYYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8837..9610
                     /locus_tag="IVK51_001930"
     CDS             8837..9610
                     /locus_tag="IVK51_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6431511.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10018..10815
                     /locus_tag="IVK51_001931"
     CDS             10018..10815
                     /locus_tag="IVK51_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP6431512.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKKIQLIQINDDRPFME"
     gene            10793..11206
                     /locus_tag="IVK51_001932"
     CDS             10793..11206
                     /locus_tag="IVK51_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431513.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11190..13835
                     /gene="mutS"
                     /locus_tag="IVK51_001933"
     CDS             11190..13835
                     /gene="mutS"
                     /locus_tag="IVK51_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP6431514.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLKKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKNSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13853..15961
                     /gene="mutL"
                     /locus_tag="IVK51_001934"
     CDS             13853..15961
                     /gene="mutL"
                     /locus_tag="IVK51_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP6431515.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPVV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15983..16543
                     /locus_tag="IVK51_001935"
     CDS             15983..16543
                     /locus_tag="IVK51_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP6431516.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARIVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
BASE COUNT         5387 a         2964 c         3598 g         4642 t
ORIGIN      
        1 ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc cataaataat
       61 aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac tggtacccat
      121 gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg tacaaaacgt
      181 ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag tgaagcaact
      241 aatgtccata caaggaaacc gagtgctaag tgagctgtac caagattatc ttggaagaat
      301 ggtgcttttg taatacctaa ggcaccaaag aataaaacga atgaaccaaa gaattcattg
      361 acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc taacacatgg
      421 ttcacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat caattgtcca
      481 atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca agggaataga
      541 ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct gattccacca
      601 aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag catccagtca
      661 ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt acctagaacg
      721 attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga gtaatccata
      781 aatacctcca aaagttataa aaatgcaaaa aaaaagaaac atcaacacga aaagcatcgg
      841 tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata aaaaaatgaa
      901 ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt aaaagttctc
      961 cacaaaccac caagcttaac tataaatata ccaaaaaaaa aacgtttttt ttctcaaaaa
     1021 ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag taaaatgaaa
     1081 gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat tattattttc
     1141 ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc tggaagtatc
     1201 ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc tgaaaaaatc
     1261 ggagcaactg gtgatttttt attgaataat ctagctattt tatttgttcc tgctggtgta
     1321 ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact gggagcagtc
     1381 gtcgtgtgtt ccatcgtcac gatggttgca gagggaaaaa cggcagaagg tgtagaagct
     1441 ttgctgggct atgtccgtaa gaaaaaagca ggaagggtat ctgtaaacga aatggaggaa
     1501 aatcaaattg actgagtttt ttgtgaatcc actatttggc ttaagtttga ctttaggagt
     1561 gtatgtatta tctgataaat ttttgcggaa atataaactg cctgttttga atcctctcgt
     1621 attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct ataaagacta
     1681 taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg cacttgccat
     1741 ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa tggctgcgat
     1801 tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact tcttctctct
     1861 tcataaagag atggtgatct cattactacc taaatctgta acgacggcta tttctgtcga
     1921 tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg tctctacagg
     1981 tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg aaagtccagt
     2041 tgctcgcggt gtggcgttag gaagtacttc tcatgctatc ggtacagcaa aagcaatcga
     2101 gattggcgaa atagaaggaa tcatttccgg acttgcgata tgcgtaaatg ggatattgac
     2161 cgttttgttg ctgccgttat tctttcagcc ttttgctggc ttgttttgat gcgaaaacca
     2221 ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa agacgtgtat
     2281 ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat tctcctccta
     2341 gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt tgttcagctg
     2401 gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca gcagtttagc
     2461 ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt ttatgatacg
     2521 ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac gctgaaattc
     2581 cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt tgcgcaaaag
     2641 cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca gcaactgcaa
     2701 acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga agaaatcatt
     2761 cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg gccatttgaa
     2821 acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt ttttaatgtt
     2881 cgtctgaacg agatcaaaaa aagacgattg gaatacatgc taggtgcctt ttttttaaga
     2941 aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat ttctgacaat
     3001 ctgttgttcc agcgattttg ccaagtcatg gagccggtat ttcctaatta ttttcaagta
     3061 gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata ttatagtgat
     3121 cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc gccctttaca
     3181 gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg gctgccagca
     3241 gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt cgcctatctt
     3301 tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa tcatttagtt
     3361 attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag acacaaacat
     3421 cccaatcatc tggcatttaa aaatcatgcg tttttgatgg gtcggtatct gactgtgttt
     3481 aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt aatgacagat
     3541 ttcccattat ttgaagagca actgttagag gagggattac ggacattttt ccgtaatgag
     3601 tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct tctgatttcg
     3661 acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt gaccgaagaa
     3721 ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca aaaaaaaact
     3781 attaaaacaa taacaaagaa ggagaaaaaa gatgacattt gaagaaattt taccagaatt
     3841 gaaaaaaggt gcaaagatta ttcgtaaagg atggagtgga tttgaacttt atgttgttct
     3901 cgtatccgat gatgaatttg atggctgtcc agtgacacct tacttattga tcaaaacaag
     3961 tgacgaaggt ttttccagct tcgctcctac agtttgtgat attttagcag atgattgggc
     4021 gatcgtagaa tgaaaaacca gtatccagat ctagctaaac aggttgtatt tatcacagga
     4081 gctgcttctg gaattggtgc tgcgcaagcc cgagcgtttc ttgaacaaga tgcttttgta
     4141 tttggattag atcaaaagtt cggtaaaatg gaagaactct ctttgcgatt tcctgaaact
     4201 tttgcctacg cactaggtga tgttcgacaa atgaaagaac tccaacaagc ggtatcgaaa
     4261 tgccaggatc gttttggtga agttacgatt ttactgaata catcggggat attggatgcc
     4321 tacaaaccat tgatggaaac tgacgaatcg ctgtgggatc ttatttatga gacgaatgtg
     4381 aaaagcatgt atcagctgac aaaaatcatc cttcctgata tgatccgccg aaaaagcgga
     4441 acaatcatca acatggcttc gattgcgggt ttggttgctg gcggaggtgg gatcgcttat
     4501 acaagtgcga aacatgcaat cgtcggattt accaaacaac tcgctctgga ttacgcagga
     4561 cagggtatcc gagtaaaagg aattgctcca ggagccatcc aaacaccaat gaatgcggct
     4621 gattttgaag ggaaaggtga gatggctgag tgggtcgcaa atgaaacacc agtcaagcga
     4681 tgggcacagc cggaagaagt agctgagctg acgttatttc ttgcctctcc tcaagcaagc
     4741 tacatacaag gaacgatcat cccaatcgat ggcggatggt tgttaaaata accttgtgtt
     4801 tttttcaagc ttctgtatga taaaactaaa gagagaaaca acggttgatg ataaaagaaa
     4861 gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt ctttttaata
     4921 atcgttaata ttcgaaacta agcggctttt cacgaccttt tgatataaac ggtgttcaaa
     4981 agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct atttttattt
     5041 gactattctt attacttgtt gcagggcagc tttttcacta ccttctgcgg cacttcagtt
     5101 ggaattttct tcccatcctt tggtcgcagt cattaccaag ataaaattat cttgcaaagg
     5161 agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca acgacagatg
     5221 tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt ttgtctgtct
     5281 ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg tccttgtata
     5341 acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatg gcttctcctc
     5401 ttggactgat agatgttgta gtaggaagcg tcagcacgct gcttgtattg cttatcaact
     5461 ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggctgtgaca gcatgcgtat
     5521 ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa ctaccttttt
     5581 tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc ggtgggataa
     5641 ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt taatttaaaa
     5701 tatggcagcg tggaagagac tttgagtctt cttccatgct gcttttttga ttaatttgta
     5761 tgaagaaatc gatttagccg ttttttcatc aagataccga tataagctgc tccagttgcc
     5821 ttgattgctt ccggcagtaa aaacggcagt agtccactgg caaatgcagt tgtaaaagta
     5881 agatcaccac ttattttcag ccagatcgtt ccgcagatca atgcagcaag agagcccatt
     5941 agattggcaa agattgcata taagtaatga gcatccgttc tttcgatcat ccaaccagtt
     6001 atccacgttt gtaagagaaa tccaatcaaa tatccgccag ttggcccaaa taaaacagct
     6061 atacctccag tcatccctga aaatacagga agaccaatca agccaagtaa aagataaact
     6121 acaacagata tcaatcctag ttttcttccg agaaaagttg ctgttaatcc aatagcaaat
     6181 gtttgccccg tcaaaggaac caagccaaaa ggaatggtca tctgagaaag gacagaaata
     6241 atcgcggcaa attccgcagc aagtattaat tctttcaatg ttcggttcat aatgccctct
     6301 tttcgttaac ttaatatttt tatttggtta acgaaattgt aaaacaaagt gattactttg
     6361 taaatagaaa atataaaaaa agaccagcta tccaatagat agcggtcttt ttcaagaaag
     6421 cttttgaaat tataagcttt ggttgtagta ttcaacgaca agagcttcgt cgatttctgg
     6481 atatagttcg tcacgttctg gtaaacgagt caatgaacct tccaattttt cagcgtcaaa
     6541 gcttacgaat gctgggcgtc caacagttgc ttctacagct tcttttactg ttacgatgtt
     6601 ttgtgatttt tcacgaactg agatcacttg acccacttct acgtggtatg aagggatatc
     6661 aacgcgtttg ccatcaacta aaatgtgacc atggttaact aattgacgtg cttgacgacg
     6721 agtcgttgca agtcctaaac ggtaaacaac attgtctaaa cgttgttcta gtaagatcat
     6781 gaagttaaca ccatgtttac cttctttgat tttgctagct tttacgaaca agttacggaa
     6841 ttgacgttcg ttcattccgt acatgtgacg aagtttttgt ttttcagtta attgcatgcc
     6901 gtattctgat tgtttgccac ggctgttagg tccatgttga cctggtgcgt aagggcgacg
     6961 tgctaattct tttcctgtgc ctgacaatga gatgcctaag cgacgagaaa ttttccatga
     7021 tggtcctgta taacgagaca ttaaaatttc ctccaatgaa tataagtttt tggagtaaaa
     7081 taatccgttg aaaaattcat aatcatatag tttgttcttc aatcttcacc tttgcagccg
     7141 cggttacaca atcgaacccg agacggggca acaaactagg gatgagttat tatttttctg
     7201 ctgcattatt ttacacaaac tacagtatat gatagatcat ccacgaatgt caagataatt
     7261 agtacataaa aacgctttac aaacctaggc tgtctttata aaatgagagg gcatgtgcaa
     7321 atggaaaaaa gcgaaggaag tttaaggaga aaatcaatga atccggtatc accattattt
     7381 gagaaaatcg atgcttcaat tgaaaagaag gcgaatcttt ttgaaaacag cttttcacgt
     7441 tacgctgttc gagcggttct cgcctgtttg tttttgactt taggaacggc tgtggcattt
     7501 gcaatcgcga tgaaggggga agatatcgcg catggtctag gaaagatttt atacgcattt
     7561 atgttcagct ggggtctggt catgattcta tacatgaacg ctgaattagg aacatcgaat
     7621 atgctttata tgaccgtggg tgtttttcgg cagagacttg ctttaagtaa ggcagccaag
     7681 attctatttg cctgtatttt tttcaatatg attggcggta tattatgtgg atttcttatt
     7741 tctatgacta cgccatttca agatttaacg ttggacaact ttatgttaga atcaatagca
     7801 gtaaaactag caaaacctgc agcaacgatt ttggtggaag ggatgtttgc gaatatcgtt
     7861 gtcaataccg ctgtactgat cagcatgcgg atgaaagagg atgccgggaa agtgatcaca
     7921 gtcgtattca tcattttcat ttttgctttt ttaggatatg aacacgtgat agcgaatttt
     7981 cctgccttta cgcttgctta ctttgcttct caaggacaga cggatggaat gacagtcagc
     8041 aatgtgttac ataatttgtt ttttgctttg gcaggaaatt atattggtgg cggtttagta
     8101 atgggactgg gatatgcttg gctgaatcag tcaaaatcat cctatgtgga ctaaaaagtc
     8161 gttcattaaa ggggaagtgc catggaagtt ttagtttttc caaaaaatga ggaaaaagcg
     8221 ttacaaatgg aaaagtacat gaaaaatctt ttttcctttg ctggagtacc taagccggaa
     8281 cgcgctcttt tacaacaacc gttactggat gagagcaaaa atttatctat ttctgaagtt
     8341 ttagcttttg tttattatta ttatgaacaa atcgaaagag agtatcagta tgtagcgatc
     8401 gatttagtcc agaaaaacat caaacggctt acatttgaag acttagtcgt attgttgcca
     8461 ctagttcaga aaaaagaatg gtgggacagc atcgactctt ggagaaaagt atacagcaca
     8521 tggtgtaaag cctacccaga gcagcgtgaa gaagtctttc aattgtttga aaaaaaggaa
     8581 gatttttggc tacgtcgaat agcaatcacg ttgcaattag gttataaaca gcaaacagac
     8641 acaggactat tgatccgagc aatagacgaa gatcgtcaaa caaaggaatt ctttatccaa
     8701 aaagcaattg gttgggcatt acgcgattac agtaagaccg atgccgattg ggtaaaaaga
     8761 cagctgacaa cagatttgag taagttagct gttcgagagg caagtaagta tttataagaa
     8821 agcgagacga caagagatga gacgcaaatt atttgctttt gatattgatg gaacgttgtt
     8881 agattcaaac aaacaagctt tagatagcac gagagaagca ttggaaaaat tacgcaaagc
     8941 cggtcatctg gtcacgattg ctacagggcg gagccgattt catgcgcaag agatcattcg
     9001 cgatcttgat tttacgaact acattttatg taatggagcg gccggttttt tagaccatga
     9061 acaagtatat aagaatttac tagatcgcga ccagttggat gcgttcgttc aagaagcgca
     9121 tcatgagcag atcgatacag cttttgtcag cttagacaat atcaagcgtt cttcttccaa
     9181 tgatgtaacg gtgatggaag aagcgatgca ttctttcggt gctgtcttgc ctgaattaga
     9241 tcagtttttt gcagaggaac aagaaatata ccaagcgtta gcatttttcg atcaaacata
     9301 tgatgggaag ttctcttcat atgataaaat tcgatttgtc cgctggcatg agaacagtgt
     9361 agatgtcgta cctaaaaatg gttctaaagc agcgacaatt ctgcacttag cagaacaagt
     9421 aggcatccct catgaagaca tcatcagctt tggcgatgga caaaacgacc gtgaaatgtt
     9481 gcgcatgtca ggaattggga tagctatggg gaatgctgtg ccagaagtac aagcagaagc
     9541 taagatggtc actgatacaa acgatcagga tggaatttgg aaggcattga aagagttaag
     9601 cgtgatttaa aagaggttgt gaaaaagctg ttctgcatca agtaataagc ccagccaaac
     9661 aaaaatagct cctcctattt ttcgtttttc tgggcttatt accgcagatg caagcttttg
     9721 aacaccgttt aaggaagagg tcgtgacaga agtggtcagt ttcaagaaat aagaagggat
     9781 ttacgaaaat tgcttctcaa atttttgtaa aattacggct tatttctgaa gaagtagatg
     9841 aaacaacgtt tattcgggaa atgggactgt gacaagatgt ttgtcatagt cccatttttt
     9901 tattttattt ttcaaaacta agaaatacca ggaaagagtt ccttttgttt gaccttttct
     9961 ttcttattga aaacaactat aatatagagt gatgttaaaa gagaagagga atttagtgtg
    10021 cgggtattgt ttattggtga tgtagtaggt tcaatgggac gagagatgat tactgaatat
    10081 cttcctagat tgaagaagaa gtaccgtccg caagtaacga ttgtaaatgg tgagaatgcc
    10141 gcttctggac gtggaatcac agaaaaaatc tataaaaaat ttttgcaaga cggcgtagat
    10201 gtcgtgacga tggggaatca tacgtgggac aaccgcggaa tctttgagtt tgtaaatgaa
    10261 gctaaaaaga tggttcgtcc ggcaaatttt ccggaaggga cgcctggaca agggatggtc
    10321 tttgtcaaag tgaaccaagt cgaactggca gtgatcaaca tgcaggctcg ttcttttatg
    10381 gtggacttgg atgatccttt tcgtaaaatg aatgagctgg tagaagaagc cagaaaacgt
    10441 acgccgatca tttttgtaga ttttcatgga gaaacaacaa gtgaaaaaca agcaatgggc
    10501 tggtttttag atgggaaagt ttcagcagtg gtcggtacac atacacacgt tcagacaaat
    10561 gatgcgcgaa ttttgccaag aggaacagcg tatctgacag acgttgggat gacaggtccc
    10621 tatgatggta ttttgggtat gcgcagagaa cctgtaatcg aaaagtttct gaccgcatta
    10681 ccaaaacgtt ttgaagttgt ggaacaaggc cgatcgattt taagcggctg tatcttggaa
    10741 ttggatgata cgacaggtca tgcaaaaaaa atccagctga ttcagatcaa cgatgatcga
    10801 ccgttcatgg agtagaaaga gaggcagttt tttgcagcag gagccggaaa ttcaaaaaga
    10861 agtaaggaaa ctgacaaaac ttttaagaga gaatgaaacg atcattcgct acaaagaact
    10921 tgaagaaaag atccagcaaa accagtattt agctgagttg agagaaaaaa tcaaacaagc
    10981 gcaaaaagat gctgttcatt ttgcccacta tgacaaacca gcagcagaac aagaagcaat
    11041 caaacaagcg gatcaattca tgcaagaatt tgatcagcat ccgcttgttg tagcttatag
    11101 aaaacagctg ttggaagcag atgatctgct tcatcattta acaactatga tacaagaaga
    11161 aatcaatgga caaatagagg aggaaaaaca tgcctcaaaa aactaaaaat actcctatga
    11221 tggaacaata tctaagcatc aaggcccaat accaagatgc ttttttgttc tatcgtttag
    11281 gagactttta tgaactattt tacgaagatg cgatcaaagc gtcacagctt ttggaattaa
    11341 cgttgacgag cagaaatcga aatgcagaag aacctattcc tatgtgcggt gtacctcacc
    11401 atgcagcaca aggatatatc gatgccttga ttgaaaaagg ctataaagta gcgatttgcg
    11461 agcaggtaga agatccaaag acgactaaag ggatggtcaa gcgcgaagtg atccagctag
    11521 tcactcctgg tacagtcatg gacagcaaag gattgtcggc aaaagacaac aattttttaa
    11581 cagctgtagt tagtcaagga aatgaatttg gctttgctta tgccgattta agtacgggag
    11641 aattgaagac ggctcggttg catgatgaag aagctgtttt aaatgaagcc tctgcattgc
    11701 agacaaaaga actcgtatta ggcagtgaga tcactgatac ccttcgtgaa cagctaagcg
    11761 ggcgtttagg gctagtcttt tctcaacaaa atgagatgga agaaaacgcg gaatttagct
    11821 ttttgacaag cgaactagtc gatccgctga aaaaagaagc aaccggcaaa ctattgactt
    11881 atttatcggt gacacaaaag cggagtttag ctcatatcca aaaagcagta gaataccaac
    11941 cagatcattt tttgaaaatg gattattatt caaaattcaa cctagaatta agccaatcta
    12001 ttcggactgg gaaaaagcac ggcactcttc tatggttgtt agatgaaaca aaaacagcaa
    12061 tgggtggccg tttactaaaa caatggctag accgtccgct tatccaggca aaacaaatta
    12121 aagcaagaca agaaatggtc gcttctttgt tagacagcta ttttgaacgt atcgatttgc
    12181 agtcagcatt aacgaaagtc tatgatctgg aacgcctagc aggtcgagta gctttcggta
    12241 atgtcaatgg gcgagacttg atccagctga aaaattcttt agaacaagtg ccaatgatca
    12301 gagggttgat tgaaggaatc gatcaaggtc aatggcaaag tctgctttca gaaatgcagc
    12361 cgatggacca tctcgtccaa ttgatcgacc aagctatcca agatgatccg ccgttacaaa
    12421 taacggaagg aaacatcatc aaagatggat tcaatgagca gctcgatatg taccgttctg
    12481 ctatgagaaa cggaaagaaa tggttggcag aacttgaagc aaaagagcgt caagaaacag
    12541 gaatcaaaaa cttaaaaatc ggatttaacc gcgttttcgg ttattatata gaaatcacaa
    12601 aagcaaatct tggaaatgca gaactggaga agtatgaacg aaaacaaact ttagcaaatg
    12661 cagagcgttt catcacacca gaactaaaag aattggagac acaaatcctc gaagcagaag
    12721 aaaaatctgt tgatttagaa tatcagttgt ttttagctgt gagagaagaa gtcaaaaagg
    12781 ctatccagcc gctacaagta cttgcaaaag caatcagtgc agtagatgtg ctacaaagtt
    12841 ttgcaacgat cagcgaacgc tatcagtatg ttcggccaga acttgtcagt gacaagcatc
    12901 agcttctgat caaagacgga cgacatcctg tagtagaaaa agtattagga catcaggaat
    12961 atatccctaa cagcgtagaa atggcagaag acgaaatgat cttgctgatt acaggaccga
    13021 atatgtctgg taaaagtacg tatatgcgac agttggcatt aactgttctg atggcgcaaa
    13081 tgggctgttt cgttccggca aaacaagcag ttttacctat ttttgatcgg atttttaccc
    13141 gaattggtgc aagtgacgac ttaattgctg gacaaagtac ttttatggta gagatgatgg
    13201 aagccaatca agccctgcgt tatgctacgc caaacagttt gattttgttt gacgaactag
    13261 ggcgaggaac agcaacctat gacggaatgg cgttggccca agcaatcatc gaatatatcc
    13321 atcgtcatgt acaagcaaaa acactctttt ctactcatta tcatgaactg acggtacttg
    13381 agaaagaact tccgcagctt aaaaatgtcc atgttggagc cgttgaaaaa gacggagaag
    13441 ttgtcttctt gcataaaatg atggatggac ctgcggataa aagctacggc atacatgtgg
    13501 caaagatcgc aggattgcct tccaatctac tagaacgagc agcagatatc ctgcatacgc
    13561 tagaaaatgg cgataacgga caccatactg ttccagctga agtagaagag aaaactaaaa
    13621 cagccaaaca ggagcaaata aaaccacctg cagaaaaagt aaatcaagac catatacaag
    13681 accgtgtaaa agaagaaaac caacagcttt ctttattcag tgaattatcc gaaaatgaaa
    13741 cagaagtcat tgaatcactg aagcaaataa atttactaga aatgacgcca atggatgcac
    13801 taaacacatt atatgaatta caaaaacaac tgtaggaaga aggcggatat cgatggccaa
    13861 aatccaagaa ttatctgaac gtttagctaa tcagatcgct gcgggagaag tagtggagcg
    13921 accagcttcc gttgtgaaag aattagtgga aaatgcaatt gacgccggca gtactcagat
    13981 tgatatctta gttgaagaag cgggactgcg gacgatccaa gtgattgata acggagaagg
    14041 cattttggca gacgacgtag agaatgcctt caagcggcat gcgacaagta agatccataa
    14101 tcgcgacgat ctttttcgca tacgaacgct gggctttcga ggggaagctt tgccaagtat
    14161 tgcctctgtt tctgaaatga ctctggaaac ggcgaatcag gaagaaaaac aaggcaccta
    14221 tctgtcgctg aaaggcggag aagttattga gcatcgacca gcaccactgc gtcaaggaac
    14281 gaagattacc gtcagcaatc tgtttttcaa tacgccagcc cgcttgaaat acgtgaaaac
    14341 actgcacaca gaattagcga acatcgggga catcgtcaat cgactggcat tgagtcatcc
    14401 atcgattgct tttcgtttag tacatgatgg aaacaaaatg ctggcaactg ctggaaatgg
    14461 cgatctgaaa caaacaattg cgggaatcta cggattatct accgcaaaaa aaatgctgaa
    14521 aatcgaagga aaagatctag attttgagtt atttggctat gtttcattgc ctgaggtgac
    14581 tcgtgccagt aggaattact tatcgacgat catcaatgga cgttttatca agaacttttc
    14641 cttgaataaa gcaatcgttg caggttatgg gtcaaaatta atggtcggtc gtttccctat
    14701 tgctgtattg gaaatcaaaa tggacccgct tttagtagat gtaaatgttc atccgacgaa
    14761 gcaggaagtc cggctgtcga aggaaaaaga actaacagag ctaattagta acgccattca
    14821 aaaagcattg tctcaagaaa atctgattcc aagtgcagcg gataatctac ggtttaagaa
    14881 aaaagtacct gatcagccta aaacagaaca aatcgaaatc gatttgtcct acgcttttga
    14941 agaagaaaaa cctaaaaagc caagtagttt gaattttgat cgagagacag gtaccttttt
    15001 cgtagaagaa ccaaagaaca catttgttga cagaattgtt gataagtctg tggaaaatgt
    15061 tgaaaactat tctggttttt ctgaagaaaa caccacttat ccagaaaaaa cagcgaaaac
    15121 ggtggataag tttggtgaaa atgttgataa ctttgtagaa aaaacagtgg aaaaatctga
    15181 aaagaaagaa tttcccgttg tggataacat ggagaaaaca aatgaagaac aagcagataa
    15241 atccgtagaa gaggatgaaa agatttacga ggaagaattg acactccatc ctgaatttga
    15301 tttcactcaa ggaaacgaca aaacattaga acaagccatc aagaaattag aaacggaaaa
    15361 accagtacaa cgttttcctc agttggaata ttttggacag atgcacggaa cgtatctttt
    15421 tgcccaaagt agcgatgggt tgtacatcat tgatcagcat gctgcccaag agcgcattaa
    15481 gtatgaatat ttccgggaaa aaattgggga agtatctgat gatctgcaag aattgcttgt
    15541 tccatttgtg ttagactatc caaacagtga tgctttgaaa ttaaaagagc aaaaacactt
    15601 gctagaagat gcagggattt atttagaaga atttggtcaa aacagcttta ttgtacgggc
    15661 ccatcctact tggtatccca atggagaaga agaatcgatt attcgggaga tgatcgatat
    15721 gctgctgaca actggaagtg tgagtgtaaa aaaattccgt gaagcaacag caatcatgat
    15781 gagttgcaaa cgatcgatta aagccaacca ttatttgaat gaagcacaag cacgtgtgct
    15841 gctgaaggat ctggcacgat gcgaaaatcc tttcaattgt ccccatggcc gtccagtatt
    15901 gattcacttt accaattcgg atatggaaag gatgttcaaa cgaatccaag acccgcatta
    15961 agtcaaagag aagagggaaa acatgaagat tgtattagca tcccaatcac caagaagaaa
    16021 agagttattg gctcatatcg tttcagaatt cgagattgaa cctgctgata tcgatgaaac
    16081 gccatttcca gacgaacagc ccgtcgatta tgttcgtaga atggctgaag aaaaagcacg
    16141 aattgtctgg gaacaagatg aaaaagaaga ccatttagtg attgccagtg acacgactgt
    16201 tgttctcaat caagagatca tgggtaaacc agaagatctg gctgaggctg aattcatgct
    16261 gaaaaaacta agcggggaaa cacatactgt atatacagct gttgttttaa aaacaaagga
    16321 acgagaagaa cggatactag cagaagcaca tgtcactttt tatcctttga cagacgaaga
    16381 gatcaaacgc tacttggaga cgggggatta tgcggataag gcaggggcat acggcatcca
    16441 aaatgctgcg agtgtattcg tcaaagaaat ttctggagat tattacagta tcgtaggatt
    16501 tccgattggt gctgtcaatc aggcactgaa aacattcatt taaacaaaaa agcggactat
    16561 ctgattttcg attgaaaatc catgatagcc c
//
LOCUS       DACCDF010000058        16525 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCDF010000058 DACCDF010000000
VERSION     DACCDF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.061"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(139..414)
                     /locus_tag="IVK51_001936"
     CDS             complement(139..414)
                     /locus_tag="IVK51_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP6431517.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(657..1967)
                     /locus_tag="IVK51_001937"
     CDS             complement(657..1967)
                     /locus_tag="IVK51_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP6431518.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(2149..3378)
                     /gene="rpsA"
                     /locus_tag="IVK51_001938"
     CDS             complement(2149..3378)
                     /gene="rpsA"
                     /locus_tag="IVK51_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP6431519.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(3513..4193)
                     /locus_tag="IVK51_001939"
     CDS             complement(3513..4193)
                     /locus_tag="IVK51_001939"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP6431520.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(4242..4877)
                     /locus_tag="IVK51_001940"
     CDS             complement(4242..4877)
                     /locus_tag="IVK51_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6431521.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(4943..6346)
                     /locus_tag="IVK51_001941"
     CDS             complement(4943..6346)
                     /locus_tag="IVK51_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP6431522.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(6333..7364)
                     /locus_tag="IVK51_001942"
     CDS             complement(6333..7364)
                     /locus_tag="IVK51_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431523.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            7395..7616
                     /locus_tag="IVK51_001943"
     CDS             7395..7616
                     /locus_tag="IVK51_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP6431524.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(7781..8518)
                     /locus_tag="IVK51_001944"
     CDS             complement(7781..8518)
                     /locus_tag="IVK51_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP6431525.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(8671..10011)
                     /locus_tag="IVK51_001945"
     CDS             complement(8671..10011)
                     /locus_tag="IVK51_001945"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP6431526.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(10248..11771)
                     /locus_tag="IVK51_001946"
     CDS             complement(10248..11771)
                     /locus_tag="IVK51_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP6431527.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(12149..12760)
                     /locus_tag="IVK51_001947"
     CDS             complement(12149..12760)
                     /locus_tag="IVK51_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP6431528.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(12883..13008)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(13090..13806)
                     /locus_tag="IVK51_001948"
     CDS             complement(13090..13806)
                     /locus_tag="IVK51_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP6431529.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(13812..14381)
                     /gene="scpB"
                     /locus_tag="IVK51_001949"
     CDS             complement(13812..14381)
                     /gene="scpB"
                     /locus_tag="IVK51_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP6431530.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(14365..15162)
                     /locus_tag="IVK51_001950"
     CDS             complement(14365..15162)
                     /locus_tag="IVK51_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP6431531.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(15132..15503)
                     /locus_tag="IVK51_001951"
     CDS             complement(15132..15503)
                     /locus_tag="IVK51_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP6431532.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(15522..16409)
                     /gene="xerD"
                     /locus_tag="IVK51_001952"
     CDS             complement(15522..16409)
                     /gene="xerD"
                     /locus_tag="IVK51_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP6431533.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         4643 a         3577 c         2798 g         5507 t
ORIGIN      
        1 aaattaaata aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc
       61 ttatttattc cttttctgct aggaaataaa aagaaaagca cttgtagccc tagagccaca
      121 agcgcttttt caactgtctt atttaacagc gtcttttaat gctttacctg gtttgaatgc
      181 aggtactttg ctagctggaa tttcgatttc ttcaccagtt tgtgggttac gacctttgcg
      241 agctgcgcgt tcgcgaactt caaagttacc aaaaccgatt aattgaactt tttcgccgtt
      301 agctaaagca tcttgaatag ttgaaaatac agcatctact gctgcagttg catctttttt
      361 agttaaatca gtagctgaag caactttttc gattaattct gctttgtttg ccatggatag
      421 attcacctcc tccacaagag atatgataga aatatcattt agttaaaact ttctgagtgg
      481 tccagaagtt taaaaagaaa tattgattaa cttcaatatt ctgttatcac gatatcatat
      541 gaaccgctta atagcaaggt ttttaccaat tttcacatgc tttttttagt catagaaacg
      601 taacaaacga aactgtcttt ttcttttgaa aaaaaaacgt cgatatggta aaatgcctat
      661 tttctcctac gagggataat tcggatggac gtcccttcaa aagtaaatgc ttttctgatt
      721 tgattttcta agaaacgttc ataagaaaaa tgcatcaatt cctcttcatt gacaaaaacg
      781 acaaatgtag gtggtttgac agcgacttgt gtagcataaa aaatcttcag gcgttttcct
      841 ttatctgttg gtgttggatt gattgctaca gcatccataa tgatatcatt caacacagcc
      901 gaagagatac gaagattctg gttcatactt acttgttcga tcatttcagg cagacgttcc
      961 aaccgttgtt tagtcactgc agaaacaaaa acgattggag cgtaatcaag ataccggaat
     1021 tcatcacgaa tctcttgttc gaaatcacgc attgtattgg tttctttctt tacaagatcc
     1081 catttattca cgacgataat aattccacga ccagcttcat gggcgtaacc cgctactcgc
     1141 ttgtcttgtt cccgaatccc ttcttcagca ttcagtacca tcaaaacgat atcagaacgg
     1201 tcaatggcac gcattgcacg catcacgctg tatttttcag tattttcgta tacttttccg
     1261 cgtttacgca taccagctgt atctatcatt aagaatttct gaccactttc agaaacgaag
     1321 tgggtgtcga ttgcgtctcg ggtcgttcct tcaatatccg aaacaattac acgttcttct
     1381 cctaaaatcg cattgatcaa agaggattta ccaacatttg ggcgaccaat caaactaaac
     1441 ttaatcgtgt cgtcgtcctc ttcttcagtc tcttcagaga aatacttcac tgcttcatct
     1501 aaaatatcac caatccctag tccatggctt cctgaaacag gataaggatc gcctagccct
     1561 aaggaataaa attcatagat atcgtttctc atttctggat tatctacttt attgactgct
     1621 aaaatcactg gtttattact tctgtataaa attttcgcaa ccatctcatc tgcgtctgtc
     1681 acgccttcgc gtccgctgac tacacaaatg attacatctg cttcttcaat cgcgatttct
     1741 gcttgatgct tgatctgatc cataaaaggt tcatcgccaa gatcaattcc gcctgtgtcg
     1801 atcacgctga attctcgacc taaccattca ccttttgcat aaatacgatc acgagttact
     1861 ccaggagtat cttcgacaat cgagatacgc tcaccagcga tgcggttaaa aatcgtagac
     1921 ttaccgacgt tcgggcggcc aacgattgca attgttggat ttgccatatt atttcctcct
     1981 cacaagtttc caatctcact tagtttaacg aatgcattgc caactttcaa gcatagattg
     2041 tcaattaaac gaagtatttt tattccttat tgaaatacta atcgcttttc aaaagaaaaa
     2101 ctgagacaaa agcagctgct ttcatctcag tcacagatac aaaatcaatt atttttcaga
     2161 atcatcggtt gctttttctt ctacttcttc tttcaacgca tcacctaaaa tgtctcccat
     2221 agtaaatcca gtattttctt ctggtaattc ataagactca acattttttg gttcttcttc
     2281 tgaagctggt ttttcttcta aagctttgat actcaatgca atacgatgtt cttctggatg
     2341 gatctctaat actttcactt tcacttcgtc accttcatga agcacttcat gtggtgtcgc
     2401 aatatgttta tgtgaaattt gagagatatg gactagccct tctactccag ggaacacttc
     2461 aacaaaggca ccgaagcttg tcaaacgttt aactgttccg tctaatactg tgccaactgg
     2521 agcttttgta tcgatttctg accaaggtcc aggctgtgtg tctttgattg acaaggaaac
     2581 acgttcttgt tcaggattga tagacaacac tttgacattt acatcatcat tgacagccaa
     2641 tacatcactt ggttttgcta catgactatg tgaaatctct gagacatgga ctagaccgtc
     2701 aattccgcct aggtcgacaa atgcgccaaa atcagtaaga cgtgcaacac gtccactgag
     2761 aacatctccc tcatggatag ttgaaagaat ttcttttttc ttttcttctc tttcgttttc
     2821 cactaccgct ttatgcgata agatcaaacg attttctgat ggttcgattt cgatgatttt
     2881 aaagctcaat gttttgcctt tatagtctga aaagtctgca acaaaatgat cttccaccat
     2941 tgatgcaggg acaaaaccac gtacaccgac gtcaacgact aaaccgcctt tgacaacatt
     3001 tgtaacaggt gcttcgatga ccttgccctc ttggaaatct ctttcgatat cttcccaaac
     3061 ttttttagca tctaaacggc gttttgaaag taggtagctg ccgttttctt tatctttgcc
     3121 gatcgtactg ataacgacta aatctaatac atcaccgact tttactactt cgctgatatc
     3181 ttctaccggc aaagttgata attcttttgc cggaacgact ccttcaactc cagccccttc
     3241 gattccgaca atcacttgtt tgtcttcaat gactaaaact tcgcccttaa caacatcgcc
     3301 aacttttact tcttgtacgc ttttcattgc gtcttccatt gactggttgt tctcttgatt
     3361 ttgatcaaat tctgtcataa cacttgtcct cctaaccaac attctgccgt aagtacggta
     3421 ctgtttttag tttaattgat tattcaaaaa aaataaagcg atttcgttcg ttttccttga
     3481 aaaaacaatt gtttttcatt ttttttctta atttaaaaaa gaagataacc ttttttcaaa
     3541 acaacagctt ttattgctgc aacgacttct ggaatcgatt tgccagtcgt atcgatacga
     3601 acagcatcct ctgcttgctt cagcggagag acgtcccttg ttgaatcata atggtctctt
     3661 tcctgtatct ctttcgtcag tgtttcaaga tcagtcggaa ttcctttttc ttggttctct
     3721 ttgaaacgtc tttctgcacg ctcacttacg cttgctacta aaaagatttt cacttctgca
     3781 ttcggtaata cagctgtccc gatgtctctt ccgtccatga caacgccacc gcgttcccct
     3841 atttttcgtt ggacagcaac taattcttct ctgacttttc catgtgcaga gacttcagaa
     3901 acagcttttg tgacatctgg ttgacggatt tctaaagtca catcttttga tgctatgaat
     3961 acatgctgtc catcttcttg ttgcgaaaaa gagataggat actttttgat cagcgaaact
     4021 agcccttctt catctgaaaa agatacattg ttcgtaatag ctaaataagt tactgcgcga
     4081 tacatggcac cggtatctgt ataaatataa ttcaattctt ttgctaatat cttggcaacg
     4141 gtacttttcc cagatgaagc aggcccgtcg attgcaatac tgatttgtgt catgatataa
     4201 ctcctttttc tcagaaaaaa agccgcaaag cgcggctatt attatttaat tcttaattct
     4261 tgaccagggt aaagcataaa gttgtttgga tctaagccat tcaattcaaa caattgatca
     4321 gtggtgatcc ctgcacgctc agccacttgt tttggacctt cgcctgcttg aacagtcgtt
     4381 gttgaaccgg ctgcagctga tgaagaaggt gttgtttctg ctgcattgct gcttgaatcc
     4441 cctgcttgat aagttgcctc acttgaggaa gtcggtgttg tctctgctgc aatactgctt
     4501 tctgacgtta cacctgtatc ttgagacaca gtagattctt ccgcgattgt actagattct
     4561 actgttgaag tctcagttga ctcctctttt gttgtacttg tagaactttc tactactgat
     4621 gtcgaagttt cagaagaagt tgctgctgtt gcattgttgc ttgatccatt tctgatccag
     4681 aaataagcac ctgctggaat agcgatacat aagaccagta aaatcaaaaa tagcgaaaga
     4741 aataatgtat ttcctttctt ttgttgtctc tgagaacttc ttgatgaaac ttcatcatct
     4801 gtatcgtaga ttggttgttc ccatggttct tgtgtttcgt tattttctgt agattgatgt
     4861 ctatcttttc tgctcactgt tttacctcct aaactctatc gtgtgtatta taccatagga
     4921 aaaagtgtct tgtcctaaaa aatcaatgtt ccatgttttt tttaaataaa tttagtaaaa
     4981 tcgtttccca atgaagttgt tcagtcttag aaaaaagctt cctctttggc agaagttctg
     5041 cttttgctcc atcaatatca caacaattct gcggcttttc ggagagtttt tcgccaaaat
     5101 aagctaagat gaattttctg cggcagtttt cttcattaat atagcgaagc atctgctgca
     5161 ggcggaattc tttttctttt tcctgacgtt tgagccgttc cacccaattt tctggttttt
     5221 cagactgctg gatcagctcc atccattttt tttgcagctc atcaaaggga gcttgctttg
     5281 tggcatattc caaataaagc tcaaaacttt gtcgctgttc tctagaaagt tgattaaaga
     5341 aataatggat acgttcatcc ccactttgat aaaaaagaat tgctgcactt tcttcctgat
     5401 ctctgcctgc tcgaccgatt tcttgtacat agttttctag actgtctggc aaatcataat
     5461 ggaccacata acggatatca gatttatcga tccccatacc aaatgcattc gtagcaatga
     5521 gaaactgcag ctgatttttt acaaactgct gttgaagttg tcttctttga gctgcatcaa
     5581 gaccaccgtg atagtatcca actgtaaaac gttccctgaa taaatgatac aatcgttcga
     5641 cttctttttt tgttgcacaa taaatgattg ccgctccttg tgctctttcc atgaagtgtt
     5701 caagttcttg ttctttttgc tgcgttttcc gaacaaaaag cgagatattt tgccgattga
     5761 ctgattgtct gatctctttt ggtggtcgat caaatagtac atgacgtata tctcttgcta
     5821 ctaaatctgt tgctgtcgcc gttagtgcca gtgtgaccgg gtttcctaaa agctttcgga
     5881 tttttcctaa ctgttgatat tctggacgga aatcaactcc ccattgagac acgcaatgag
     5941 cctcatctac tacatataac gcaatatttt gttttttcag atgctctaaa agagaaggat
     6001 tagtcaacat ttcgggactc aaaaacaaaa atttatattg cgatagatga cggaggacgt
     6061 atcttctttc agattgcgaa agtaaactat tgaaagcagc tactcgtttc tcccctcgtt
     6121 tttgtaattg cgtcacttga tcttccatta gagaaagcag cggtgacacg atgatgacca
     6181 gaccttccat taaatacccc gtcaattgat agcaaaggct ttttcccgtt cctgtaggca
     6241 aaatagcgag agtatcttct ccatcaagca acgcctgaat aatttcttct tggccgggac
     6301 gaaatgaatc aaatccaaaa tattttttta attcatttat taacattctg tatcgcttcc
     6361 tttctcaata gatagatttg atacaaacga aattctccat aatctaattg tccatctaca
     6421 ttccattcgc taaagcgcca ctctcgcacg gaatccttat ttggttccaa acgttcgatt
     6481 gtttctgcag agagaatcca ctcgtatgga aaatcatcaa aaaacaaggc ccactcaata
     6541 agatgatctg ttactgttcc tctttttaaa tgacggattg ccatgatctg gtcaattgtc
     6601 tgaccattca taaacatctg cttggtgatc atcatgcttt gattgaaatt ttgctgtaat
     6661 aatgaatcga tcaaagcgtg ccaaagcgaa tctggccgtt tttcgatttg tgctaacaaa
     6721 aggtggatac atctgctttg ttgcagataa acctctgact catcattgtt ttttgccagc
     6781 tgataaggaa gcagccctgt tcgctcattt ccagataatt ggttagctaa aaaatctgct
     6841 gcttcttctg gtatctccga aaacagctgc accaaactct catacgcagt atcaatcaaa
     6901 atttcacgtg gcaattggct ttcactaagc cattttttta gttggaaagt ataaaaagga
     6961 cttgtctctg ctggaagata gtcttgaata cccgttgcca gattagaaat tacttgaaca
     7021 gcaaatttaa tgagccgcca actagtttcc cctgttctgc catagcggtc aaaacgcaaa
     7081 ccagtatgtt ctatttgatg ttcggacaac atatcagccc ctgcaggcgt tagctttgct
     7141 tcattttcat ttatttcgat ccaaccttgc tgcgcaattt tttgcaaaat ttgataaaac
     7201 ttatcctgct tcaattctgg aaaactgtta tggataaaca aaagttcgtg ggtaaatcca
     7261 taaataagta ctgaagtcgt tcgttttccc acaaggagtt ggtaaagaga actaatccgc
     7321 agcttgtgtc ctgatgaaaa tagcgataaa ataaaaacaa tcatattatt ctccttaagt
     7381 taggatggtt agaaatgtca ctactatgta aacttgtacc tgaacgctgt attgcatgcg
     7441 gtctttgtca aatcgaagcg ccagatattt tcgattatga tgaagaagga atcgttttat
     7501 ttgcccaaga accagaggct catcaacagt tcgtgatgcc ggaagaagaa acatatgtaa
     7561 aaaaagcgta tcaaaaatgt ccagttcgag cgattttatt agaaaataaa atataaatat
     7621 aaacgaaact ttaaaaacgt acactaagtt ctctaaactt gaaaataagc cgggatctgt
     7681 ttctcttgtt ccgtgaaatc ccgacttatt ttgaaagatt catttgctat ctatagttaa
     7741 ttgactagtt ttttcacgga ccctatagga cccatttata ttatttcaac tttttcagcc
     7801 agttttccaa tgtattgacg atcacagttt gctggtctgc gttagaaatt tctgcctctc
     7861 catctccttt ttgctcacca tagctgccaa aacctccatg atttccgcca ttgattgata
     7921 catactctgt tctttccggt aaatactgct tagcttcttg ataagcttcc tgattcaata
     7981 ctttatcttt tgtcgccgtg atcgataaaa caggaacagc tgattctgtc aatgttcctt
     8041 ttttgtcggg ataacttgcc atgaagaata ccccttttaa atgtttgtct gaatgattct
     8101 tattgacata acgacttgcc attacgccac ctaatgaatg gccaccgatc acataatcgg
     8161 aatttggaaa atcttctttg accttatccg ctttgtttcc gcccatcact gctagatcaa
     8221 gtggcatttt gacaatagcg actgaaaagc cgttttttgc taaatcttgc gcccaaatac
     8281 tataactttc tggctctacg agagcaccag gataaaaaat aagtaatgga gactttccat
     8341 gattcttttt ctctccttca aaaaataagt aatcttcttt gttttttcct tcttttacca
     8401 acacttgcgc ttctgacgat ggttcgtacg tattttgctg tatatagtaa taaccggctc
     8461 caccgagtaa tcctaaaatg atgactaagc caattagtat tcgtctaaat attttcactt
     8521 tctcccacct tttctatctg agtgacccct ttttcttaat caagtatgat tataccttaa
     8581 aaaaataaga gagaaatgca aactttccag catcaaataa aaaaagacgg aatatccatt
     8641 tgaatatccg ccctttttgt atgtttattt ttatttagca cataaacgat acacagcttc
     8701 tgcataaatt tccattgctt tatacatact acttaattcc catcgttcat tcgcctggtg
     8761 cataaagtca ggagtatcag ggaacatcgc accgaatgcc acacactggt tcatcgtacg
     8821 agcaaaagta gcgccgcctg atacaaacgg ttcagtcatg tctccagtaa tatcgcgata
     8881 tgtccccaat agatttttca ctaactcact gtctagtggc acgtataatg aatcaaggaa
     8941 gtcaaattct tcataagtca acccatattt ttctgctgtt ttagttaatt tagctactaa
     9001 atcatctttc ttgaatgtaa cagggattct catatctaca ccgatttttg tttgttctgg
     9061 tgtgatttcc aaacttgcaa agttcatcgt tagctcgccg gattgttcat cttctgtttt
     9121 gcccacgaca gcttcaccag ttgcattttc ttgaactaat tttcctaaga aattgattgg
     9181 gccaaaatca aatacttctg aaagagcaat tgccagacga gaaatagcat tcactccttg
     9241 tgctgcatct ttcgcgtgga tacttttacc aagaacgacg atgccttcac cttgttcttc
     9301 gaaatcgaat tcgtgttttt tcaatgcttc ttttacttca gaaagctttt ctccagagta
     9361 aggagctgca tcaggaacaa cattcaacgc tcctccagct tttacagaaa actcgttcgt
     9421 tcctggtcct gtcaagtaag cttgcaataa gcctttttct gcataaatta aaggaaattc
     9481 agcatctggc gcaaatcctt gcgtaattcc ttcttctttt tcgttatatt tttctaaaca
     9541 acgccataaa ttttcttcat ctgtaccaaa gataaaacga atacgtgtct tgaattccac
     9601 gccagcatca atcaaagctt tgacggcata cattgccgca atagaaggac ctttgtcatc
     9661 ttgggatccg cgaccaacta accagccgtc ttttatagtt ggatcgaatg gttttgtttc
     9721 ccaattattt tcgtcccctg ctggaacaac gtccatatga cacaagatac cgaataattc
     9781 atctccagag ccaatctctg aataaccgta atagccttct ggatctttaa atgttctaaa
     9841 tcctagcttt tcactgattt ccaatacttt atccaaagct tcaatcactt ttttaccgaa
     9901 gggatgtcct tgtcctgaat ctgcttcatc tagtacggaa ggaattcgga tcaattcgct
     9961 caatgattct aatgcttgtt catgatgttg ttctgtcaca aattttttca tgatattatc
    10021 ctcctaatat gttaaaaagg gtcatgtcaa attcctccga cacaacctct tcttctcaaa
    10081 taatgacgtt ttaagcctgc tccatcagca agcagaatgt tcttaacaaa tgatgaaaga
    10141 aaaaacactc actgaatgga gctattgact tgtccacgtg cttatcgata gccgtttatc
    10201 ggtttcttca ttcttatgat agaccttgta cctgtatttg tctaggttta gaacattgct
    10261 gctacaccta ggatcacaca gatggctaca aataatatcg caattaattt accggtaaat
    10321 ttccaccaca ctgaaacatc gaaacgagca attgctacag cacccattac gatagcagat
    10381 gttggtgtaa ttaagtttac taaaccagat gcagattgat aagcagtgat tactaaatcc
    10441 ttaccgacgc cggcaaattc acccataggt cccatgattc ccattgttgc agcagctaag
    10501 ccggaagttg aaggaatcaa gaatgacata gggatataga aaataaatgt aataatgatg
    10561 aatacacttg aagataatga acttaatcct tgttcgcccc aatgtaagat ggtgtctgtg
    10621 attaatccat tgttcataac cacttggata ccacgagcaa cagctactac gattgctaca
    10681 ccgatcaaat cagaagcacc agctaaaaat tcagagacaa attcagactc tttcatgcca
    10741 taagtgattg ctactacgat ggacattact aagaataaca tagtaatttc tgggaagtac
    10801 cagtcaccta atggaagcat gcttttacct agaaccgtac ctagtaccgg aaggtttgtt
    10861 aaccagtttg tcaatgattc aaaaatcgtg aaattcgcat tcaaggttgt ccaaggaatc
    10921 aaactgatga tcatgatacc aaaagtaata aagaacaata cattaacatg tttttgtttt
    10981 ttcgtgatta cttcttgacg accgattgct tcaatatcga aatgcttcat atcttcttta
    11041 cgttggttgt atactaatga ttttgttgga tctttttcga tctttgatgc ataacgatac
    11101 acatatacaa tagaaatagc taacaaaata acgaacatca agaatcgaag accaatacct
    11161 tcacccatcg aaatcccaac agcttgtgaa gctacgcctg ttgcaaaagg atttaccgtt
    11221 gaagctagac agcctacttg agaccctact aatacgattg ctacagctac tactgtatca
    11281 aagcctacag ccatcatgac tgggatcagt aaagcataga atggaatcgt ttcttctgcc
    11341 attccgtaag tagagccgcc taatgcaaat aacagcatca atactggaat aagcattttt
    11401 tcttttcctt tgaatttctt cacaactgaa gcaatcccgg catctagtgc ccctgttttg
    11461 ttgataatac ctaaaaatcc accaacaacc aaaatgaaga aagagattgg gattgcagca
    11521 ggtgtttctt tagttagctc ccccgctggt acacctagca tcccattgat tggcgccatg
    11581 aaaatatccc acagcccttg aggattttgt tgtactcgtt catatgatcc agcgacgatg
    11641 ttgcctgcat cgtctacact atagtgccca gctggtataa accaagtaag tacagcgatt
    11701 actgcaataa taataaataa gaccgtatac gctgaaggca tttgaaattt tttctttttt
    11761 gtttgttcca tcttcttctt cctttctttt ccgttagaga caacaatttt ggctctaatg
    11821 ttattttatc aaaaagagta tcagaacaaa aataataaca cgcattttgt aaaacttttg
    11881 ttcaaaaagt tcgtttttcc ctattccctc ctcttttttc aggtttttaa agctttcttc
    11941 tttttcattg tagcgtttcc ttttttttat aaacaaacat tttcgtcttt taaaaggaga
    12001 agaatttcac atttgcaaag aattgattga tttttatgca taaatcaaaa aaatactcaa
    12061 aacaaacaaa taaaacgttt acattattgc atgtcttgta tttttttcca aaaaaaaaga
    12121 ccaagaaatt tcttgatctt tttgatattt atttgactgc tgtatgacgt tgttcgaact
    12181 gatgttgttt tcgacttaac catggaagaa gttgcgcatg cagcactaaa aagacagcac
    12241 tcacgatgaa tcctttgatc aagttgaaag gaacgatccc aatcagtaca tagtttgcta
    12301 aagacatgcc cagcatctgt gttgcgttca gccctaaaaa cattaagtaa agaggcgtaa
    12361 taacgaaata atttgcgata ctcataaaaa tagttagagc gatggttcct gtaatgacac
    12421 ctaaaaattt gtttcttcct ttatgctgtt tctttacact aaagaaataa taaatcggga
    12481 atgtaaagat cgttgttgat aagaaactag cagtgtctcc taccaaatta tccggagaaa
    12541 aacctgtagt cagcagatgt aatactgaac gcaagaacgc tgttgcaact ccggccgctg
    12601 gaccgaataa gaacatgctg atcaataccg agatatcact aaaatcgatc ttcaaaaaac
    12661 taaaagccgg catgatcgga aaagcaaaat actgtaaaac taatgcgatc gcagcaaaca
    12721 tcgctactgc aaccattttc tgtacacggg tgttcttcat ttgaaatcct cctgttagga
    12781 ctcctcttca gcgaactttt tgacgtttct ctccaaatga aaaaactcta ttttccaggg
    12841 aaaatagagt tagagttatt tgatcaagcg tcgtcaaata agctctatct tctttatcca
    12901 gactatactg tcggtatcgg aatttcaccg attcagctat ttttttgcaa aataggtcgt
    12961 ggactataac caccggtcgg gaatttcacc ctgcccctga agacgaacct tatataattt
    13021 ttgtttacat attcattata catatctatt caaaaaacgc aacatctaac acttataaaa
    13081 agtaagtgtt cattttgagt ggttcagcaa actgctgact tcttttttgt tcagttcacg
    13141 gtaatcccca ggacgtaggc cttgtaaagt cagatctccg tatttttcac gttttagttt
    13201 ttgcacagga agaccaactg cttgaagcat atttttcact tgatgattac gtccttcgtg
    13261 gatcgtcaat tccacgatac tgtggtttgt cttaggatcc actgataaaa tttggaattt
    13321 agctggagat gtttttcgtc cttcgatctt tattcctttt gtcaaaggtg caagcatcga
    13381 ttttgtagcg atccctttta cctttgcaac gtaaacttta tccacttcat gttttggatg
    13441 tgtcaggcgc tgagcaaaat ctccatcatt tgtcagtagc aagatccccg aagtatcgta
    13501 atccagtcga cctacaggat agatacgttc ttttaccatc ggcaaaagat ccgtaacaac
    13561 ttttcttcct ttgtcatctg caacagcaga aataactcct ttaggtttgt acaataaata
    13621 gtaaccgtat tcttcttgat aaattgggac ttcatccact tccacagtgt catgtttccc
    13681 aacttttatc cctaattccc ttacaacttg tccgtttact tttacacgtc ctgcaaggat
    13741 gaattcttct gcttttcgac gagaagtgat gccagcgtgg gcaattactt tctgcaatct
    13801 ctccattaca attattctcc tttattcatt ccatcaaaga atagatctgg tgacacgtct
    13861 tcttcaagtg aaatttccat ttcttgtgta tcaggcagtt cttctaatga tttcaaacca
    13921 aaataatcta agaagtagcc agttgttcca tataatatcg gccgacctgg tccttctact
    13981 ctaccttttt cttcaatcag tttatgggca gctagacgct gtaccgctcc tgaaacctga
    14041 actcctcgaa tctgctcgat ttcagctctt gtgattggct gtttataagc gatgattgct
    14101 aaggtttcga cggcggcttg agataatgtg tttgatgtcc cttgcgcata tcgcttaagt
    14161 aagggagcca gctctttttt agtcgttaat acataatggc tttccgtttc aaaaatatgc
    14221 aaagctgagt gcgagttttg agtgtaagat tcttttaatt ctttcactaa acttgtgact
    14281 tctgcttcgc tgagatttaa cagtccgctt aattcttcat tactgatccc ttcctcgcca
    14341 acaataaaaa gaagggcttc aagttcactt aatttcgtca tgttcggtat ctcctactgc
    14401 tggataaagc ataattgatt catagttttc tttttgttgc gcaatgatct gccctttttt
    14461 catcaattct agcaaagcca taaaagtagt gacaacttca cttttagaag gtgactcaaa
    14521 aaaagaatcg aaagtacagc catgcgcatt tctttttata cgctcttcga taaataccat
    14581 tcgttcttcg atcgttgtgt catcgggcgt gatcgttttt tccacttttt gagaaaattt
    14641 cttacgttct aagattttct gcatggcacg gatcaaatcg atttttttga cttgtcctcg
    14701 cttcaacggc ttttcagcat ccgctaaccc atcgatgttc ataggttctt ttgtgtaata
    14761 aagacttcgt tcctctgctt tttctgtcag tctttcagct gcatatttat atttacggta
    14821 ttctaaaagc tgagtgacca attcttctcg aggatcttct tcccataatt cttcatcgtc
    14881 tgtgccgaat tcttggacag gaagcaatgt cttgcttttg attgccatca atgtagcagc
    14941 catcactaag tattctcctg caacagctag ctcgagtgtt ttcattgtat ggatataatt
    15001 catatattgc tccgtgatcg tagcgatcgg tatatcatat atatcgattt ccatttgctg
    15061 aatcagatga agcagcagat cgagaggacc ctcgaataca tctaatttaa tattgatctc
    15121 cgtcaactca ttcactcttt cttctttttc tttgctagcc attttccaat gtaaggctct
    15181 gtgatttcag ttgcacggat ggagacatcc gggtactttt cctgtaattc atctaacatt
    15241 tgaaagccca tattctcacc gcgataagac ggattcaaag aaatgtcatg gatgatcagc
    15301 tgatcgttcg ttgcccagta aataccaata atcccttgaa tgttggttga accttctggc
    15361 gtaaagaaaa aaagttcata atcgtcttca gattcgtacg tatcaatctc cttcaacaaa
    15421 gaagttttac ctgtcagttt cttatggaaa ctcaatagtc ccatagcaat tttacggttt
    15481 tctttccggt aatgtgttaa catcttctca ccttcctatg actaagccct tggaaaatgc
    15541 tgcttataaa catccgtcat tcttttcttc gtgatatgtg tataaatctg agtcgtagag
    15601 atatccgcgt gacctaacaa ttcctgtact gtccgcaaat cagctccatt ttctagcaga
    15661 tgtgtggcaa agctatgccg cagcgtatgt ggtgttactt ctttgtaaat cccagcctct
    15721 cgtactaact gcttcagatt tttccagatt ccttgccgag aaagaccttt cccatgatta
    15781 ttgacaaata catgcatttc agatgcgtcc tttacaagta atggacgtgc ttcgtctaag
    15841 tagcgttcca gccattggat cgcataatca cctaaaggaa tgatccgctc tttatctcct
    15901 tttccaactg tctgtaccag tccaagagaa agatgaagat ctcccaattt caatccaatc
    15961 aattcactga cgcgcatccc agtagcatac atgacttcca agattgcacg atctcttatc
    16021 cctaacgttt ttgtcgtatc aggtgcttca atcagctttt ctacttctgt cagagtcaat
    16081 gtacttggta atttctgtgc tttcttcggc gtatcgatat gttgcatcgg atcatgatca
    16141 gtgatacgct cttgccgtaa aaactgatgg aatcttctga gacttgagat catccgagta
    16201 acagttgctg tcgctttttt ttcttcatgc aacttctcca gaaaacggat gactaaaaag
    16261 cgatcgacat cctgccatga agcaattttc tgctcctgta gaaaatgcaa gtactgttcc
    16321 aaatcacgtt catagctttg tcgtgtattg gcggacaatc cccgttcaat attcaagtag
    16381 tggagataat cgttgatttg ctctttcata ataatccccc tcatgcctct ctatcatatc
    16441 agataccgct tcattctgat agtctttttc cataatctga gcaaagaaaa agccgtctag
    16501 acactcagac ggactggttt ctttt
//
LOCUS       DACCDF010000059        16358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCDF010000059 DACCDF010000000
VERSION     DACCDF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.062"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(143..799)
                     /locus_tag="IVK51_001953"
     CDS             complement(143..799)
                     /locus_tag="IVK51_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6431534.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            992..1270
                     /locus_tag="IVK51_001954"
     CDS             992..1270
                     /locus_tag="IVK51_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431535.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1289..2497
                     /locus_tag="IVK51_001955"
     CDS             1289..2497
                     /locus_tag="IVK51_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6431536.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2645..3010)
                     /locus_tag="IVK51_001956"
     CDS             complement(2645..3010)
                     /locus_tag="IVK51_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP6431537.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3157..3930)
                     /locus_tag="IVK51_001957"
     CDS             complement(3157..3930)
                     /locus_tag="IVK51_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6431538.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4104..4964)
                     /locus_tag="IVK51_001958"
     CDS             complement(4104..4964)
                     /locus_tag="IVK51_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP6431539.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4964..6382)
                     /locus_tag="IVK51_001959"
     CDS             complement(4964..6382)
                     /locus_tag="IVK51_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431540.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6439..6888)
                     /locus_tag="IVK51_001960"
     CDS             complement(6439..6888)
                     /locus_tag="IVK51_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431541.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7333..7596)
                     /locus_tag="IVK51_001961"
     CDS             complement(7333..7596)
                     /locus_tag="IVK51_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP6431542.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7695..8990)
                     /locus_tag="IVK51_001962"
     CDS             complement(7695..8990)
                     /locus_tag="IVK51_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP6431543.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8987..10258)
                     /locus_tag="IVK51_001963"
     CDS             complement(8987..10258)
                     /locus_tag="IVK51_001963"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP6431544.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10423..11292)
                     /locus_tag="IVK51_001964"
     CDS             complement(10423..11292)
                     /locus_tag="IVK51_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP6431545.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11584..13194)
                     /locus_tag="IVK51_001965"
     CDS             complement(11584..13194)
                     /locus_tag="IVK51_001965"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP6431546.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14387..15727)
                     /gene="glnA"
                     /locus_tag="IVK51_001966"
     CDS             complement(14387..15727)
                     /gene="glnA"
                     /locus_tag="IVK51_001966"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP6431547.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15755..16147)
                     /locus_tag="IVK51_001967"
     CDS             complement(15755..16147)
                     /locus_tag="IVK51_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6431548.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4666 a         3469 c         2783 g         5440 t
ORIGIN      
        1 ggctcacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
       61 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaacgct
      121 gatttcacaa cctctttgtt catcaaaaga taatattgtc ttctaacaag tccatattca
      181 aaataacgat atgatggtct ttaacggtga taataccatc ttttttcagt tgttttaaaa
      241 ttctgtttac actgctagca gttgagatgc cacaaaattt ggcgatttct tcattcgtga
      301 tcgaaaaatc gatcagcatc ccttcttctg tccgcacacc gaataaatca tataattcgt
      361 aaagttgcgt acaaacagct cccagctttc cgtttaatag catttgctgc atttttttca
      421 tcgaataaag cagacgatag cggtaatact cttttacata gtgttgtagc tttgggtctt
      481 ggttgatatt ttgccaaaag cgcacacgat tgattcgata aaactctgca tattctgatt
      541 caactcgaat attatacggc gaatcgatga attgtgaata ttcgtctttt aaaagagaga
      601 cgatttcaag atctttgata tagcgaaggt taaattctct cccatcattt gaaataacac
      661 ttgcttttac cgttccctct ttcaagatat acgtatggga atcttccatc ccttcgaatg
      721 tcaaatattt atgatatcct ttgcgtataa tcggaaaatc atttttatcc aaatattcct
      781 ttaagactct actatccaat gatgctcccc catccaaact ttcatctaaa aagatcatac
      841 agtactttaa aactttttaa agtccaaagg tattttacta atgatattaa catttgttaa
      901 gtaccttttt ccatcactta tgcgattttt ttcctttttt ttattcgtta tactgcagaa
      961 gtaaaatttt ttaagaatag agggaaaaaa catgaattac tggatgaaca caatcattaa
     1021 tcgattagaa acagcatatc aaacaagatt tgatatgaaa gcttctttag tctttttaaa
     1081 tgatgcgtat caaaactcga tcgaattgat caaagcagtg gatgagaatc caacaaacga
     1141 atgcgaagaa tttttaaatc tgtttatgtc tacaagagat ttatttattc gccaacttgt
     1201 tgatcgctat ccatccaatt atcacgatgt agaagttcaa atacaaaaat taaaagcata
     1261 ttcagcttaa ggggaatgta taacatacat ggaaaagaaa ttatttaata aatattttat
     1321 tggaatcaca ttgatcaatt ttattgtcta cctgatttat tatctgttga tggtcatcat
     1381 ggcagtcatt gcgcaagaca ccttacacgc cagcttgagc caagcaggtt ttgcttctgg
     1441 gatctatatc atcggaacat tgctggcacg cttattcatg gggaaacagt tagaactatt
     1501 tggtcggaag tttaccttaa gaggcggcgc gatcttctat ctcctttcaa cattagcata
     1561 tctttatacg ccaactatcg gggcattgta tttagttcga ttcttgaatg gattcggcta
     1621 cggtacagtt tctacagcta caaatgctat cgttactgca tatatccctg catctaaaaa
     1681 aggggaaggc atcaattatt atggattaag tactagttta gctgccgctg ttgggccatt
     1741 tttaggaatg attttgctga atatgactga ttttcgtttc attatctggt tttcaattgt
     1801 actggtcttc tttgttacga ttgcctgcct tgtctttcct gtaaaaaata ttccattatc
     1861 tgaagaacaa aaaatatctc ttcgctcatg gaatatcaat agttttattg aaaagaaagc
     1921 acttttcatt acaggaattg cctttttgat gggtctttct tattcaagtg ttttatcatt
     1981 tttatcttct tatacaaaag taatccactt agttgctgct gggtcatttt tcttcgtcgt
     2041 atatgctttg gtcatcacat ttacacgtcc attgactgga aggattttcg atgtcaaagg
     2101 agaacaatac gtgatgtatc ctagctattt attcttaaca gcaggattat ttttactgag
     2161 tgtaacgacg aatagtgtga cattgcttat ctctggagca ttagtcggcc taggttatgg
     2221 tacgtttatg tcaaatggac aggctgtttg tttaaaaatc gtccgcgaac atcgaatcag
     2281 tattgcttta tctacctact ttattggttt agacctaggc ttaggcgttg gtccttattt
     2341 gatggggatg ctaaaatctc ttacttcatt tagaggattg tatgtgattg ccggagtcat
     2401 tccattgata tgtacagcac tctatcttgt taatggccga aaacatagtc atgaagcaga
     2461 gggcgaattg attgaatcat tgaaaaatga aatttgatct tgatgaatag ataaaaaaaa
     2521 ccgttcagat ctgaaaataa tctgagcggt tttttgagta aaaatagttt atgattattc
     2581 ttaattaaac atctatttat ttttaaagct ttcatttatc gataagaata gtttaactgt
     2641 tgatttattc cattttccgt tcatctggta cgatgccttg acttagcaac cacttgtgga
     2701 agaaccattc tgccacaccg atcaatagag caggaatcaa tactttcatc caaatatcga
     2761 ctgtatcatt cataaaatat agatagagcc acaagataac tgcagctgaa ccagcatctg
     2821 cgatcgtcgc tgcgatgttc cctactttac gtaagatcaa cagatcccct agcacataga
     2881 tcatgactac aataattacc ccaacaatcg ttgaatccat aaagctaaca ccgtataaac
     2941 ctgtcagcac gacccacaga atcgcccaga tcatgattgc cttcactacc aacgccttca
     3001 catgtttcat tttctgcact cctttctcct ggataaaaaa gtaagattat ctaacaagat
     3061 aaccttttca ttattatacc atcggctgag agaagcgagc aaagataagg tcctatctaa
     3121 aaatgaggaa gccacttaga acattcacgt aataatttat ccgtacagac attcgatatg
     3181 gtgttccttt actttcgcat actgttcttt tgtcagttct ttacttgtca ccagataatc
     3241 gatttcatga aaagcaatgg cttgatgcat gaatttatgt gcaatttttt gctgtgtaac
     3301 taaaacggca agccggtctg attgttttct aaaagctttt ttgaccattg cctctccttc
     3361 atgagtttct gttactcctt ggctgaaatc tagcccgcga caactcatta tcgctaaatc
     3421 cgcataatat gacaatgcat cgttataggc ttttgaaccg ttgattgttg ctcgtttcgg
     3481 aacaattgcc cctcccaata cagatacttt tgccgttgta tattcactca acatagcagc
     3541 tgtgacgatg ccattcgtaa tgatctgcaa accatcaaat cttgctaggt ttctgactaa
     3601 gtgtaaagag gttgtactgg aatccaagaa taagaccata tcatctcgaa tcaaagaaga
     3661 agctgtatct gcaatcttac gtttttctgt ttcttcttct aatttggtga tcttcatcag
     3721 gtaaggcacg tcaatttttc gattgtcaat aagcatggcg ccaccgcgaa ctctacggat
     3781 caatccactt ttttccagaa aaacaaggtc acgtttaatc gtggcttcgc tataatttac
     3841 taactcgatc aaatcggtga ctcttgcaaa ttggttgttt tttagataat tcacaatctc
     3901 ctgctgtcta ttctccactt cattcaacat aaaatcaccc catttacttt atatagtaac
     3961 aggaaaaaag aggttgtgac agaagtgatt ggcttcgaga aataagaaga aatttttcga
     4021 aaaaactact tctgaacatc gtgtgtggtt aagagggtat gacaagacta ttgtcatacc
     4081 ctcttgacca cacattttta tttttagtat aaactagcct tgcctactgt gttcaaaatt
     4141 tccattttat gtttgaccac ttcttgtacc tttttattag ctgctggata aattgcattc
     4201 ggctcgtaca tcgatggatt ttcactcaat atttctctca aagcattgaa aaagacgctt
     4261 ttaagatctg agctcaaatt cactttggca actccgtatt ttactgcttc gctgacttct
     4321 gcgtccggat tacctgaacc gccatgtaaa acaaatggaa gatcgatttg ttggttcaat
     4381 tctttcagta agtcgatatt taattttgga gttctgtttt tcggatacaa gccatgcgct
     4441 gttccaattg ctacagcaag tgtatcgatt cctgtttctt tcacaaaaat cttcgcttga
     4501 tcaggatctg tataaatgat tgtttcagca cctccttcag cagaaccatt attcccaatc
     4561 gttccaagct cagcctccac tgagacattt accgcatgag ctaacgcaac cacttttttc
     4621 gtaatctgta cattgtcgtc gaatgatttc aaagacgcat cgatcatcac ggaagaatag
     4681 ccgtttcgaa tcgcccgtaa gacatcttct atcgtaccgc catgatccaa gtggataacg
     4741 atcggcagtt tacttgtata agcataggct cttactgccg caacaaactc atcacttaaa
     4801 taggcgattt catcaggatg aatttctaag attactggtg cttctaatct ctctgcttct
     4861 tcgatgattg ctttcatcat atcatagctg caaatattga aggctggtac tgcaaattga
     4921 ttctctttag ccactttcaa taactctttc attgtatata acattacgct tccacctcat
     4981 ttgttagatt ttcatttttc gtccacttgt cttgtttttt ctctttcact gtttttttca
     5041 atagtccaag cataaaggct gtagtgactg tacctgatgc aattgccaag acatacatag
     5101 gtaaattgtt cataacattt ggaattagca tcacccaaat ccctccatga ggtgccattg
     5161 aagtaactcc tactgccatg gaaatccctg cagcgactgc tgaacctgtc atcaaactag
     5221 gtatcacgcg gatcgggtca gataccgcaa acggtatagc accttctgta atatacgaag
     5281 cgcctaaaat ccagcatgat tgacctgaag tccgttcttc tttggtaaat ttatgtttga
     5341 ataataaagt ggctaaagca atacctaggg gaggtgtcat tcctgcagcc atacaagcag
     5401 caatcggtgc atttactcca gctgacatca aaccgatcga gaaagtcgaa gtcgatttat
     5461 tgatcggccc acccatatca gctgccatca tgatcccgat aagtaatccg aacaatagcc
     5521 cggatgtatt tcccaagcta ttcagccaat ttgtcaaagc atccaacacc catgtgatcg
     5581 gttgttcaat cacaaatacc atcaaaagcc caataattaa gatcgataaa aaggggacga
     5641 caatcagctg ataaatagca gaaaattctt tttttacgct aattttattt gccagaaaat
     5701 cacagatata ccctgctagc acgccaccaa tcattccgcc aagaaaacct gaaccacctg
     5761 tagaggccag taatccggca acaattcctg gggcaaaacc tgcttttcca gcaatcgatg
     5821 tagcaatccc cgcgcctaac gcaggaacca tcatggcaaa cgctgtatct cctccgatcc
     5881 ttgaaaatgc agctgctaac acattatagt ctgctgagtt gggatcagcc gctgtaatcc
     5941 caaaggcaaa actcagtgcg atgatgattc cgcctgcgat cacaaatgga agcatgtaat
     6001 tcacaccatt catgaaatga ttatagattg tcggcttttt ctcatttgca gctggttctt
     6061 tttcattaga cacaacgaat acgccaatat ctctcaatgc ttcgtctatt actacttgcg
     6121 gttctttgat tgctcggctt gtagagacac gctttacttt tttcccggca aatcggtcca
     6181 attcgatttc tttatctact gcaagaatga ttgcatctgc ctctttgatt tcttgttcag
     6241 ataactgatt ttctgttttt acaccttgtg tttcgaattt tgcttgatgg tttagatttc
     6301 ttgctgttac ttctaatttt tctgccgaca tataagtatg agcaattcca gtcgggcaag
     6361 cagttacacc aacgattttc atgattattt tcctcctttt tcttcactat cgatactcgg
     6421 acaaagactc tactattttc attcttgact aatctgtctg atgatatctg ctggcgtagt
     6481 ggcttctttt attccctttc taaaatctgc attcatcaat cgtctggaga gtgtgctgag
     6541 aatctctaga tgcatatctg ctgatttttc aggaactacg atggcaaaaa tatattcaat
     6601 tggcatttca tctatcccgc tatagtccgt taatttggct gtatgaaaaa aacacagtgc
     6661 tggttttgtc acatatttgc tttttgcatg gggaatccca aatccatctt ctattcctgt
     6721 ggagatctcc tcctctcgtt tgaatagtgc cttaacgact ttttgaacat ttcttacctt
     6781 atttgttttt ttcaacacat cccctattcg ctcaaataat gatccttttg aatctatcga
     6841 tggatcgaaa acaatcagtt cttctgcgat caattgatca aacctcataa aaaacctctc
     6901 ccccacaaaa aatgttaacg ttttcttgga actgaggtca gtatagcatc gctagctgta
     6961 ccgttcaata ttatgaatga acagaggaaa aagacccatc atccgcgtgt ataagtcatt
     7021 aaaatgaacg atctagctca caaacaatcg caagtctata acaaaacaaa aagccttcct
     7081 tgaaaggaaa gcttagcttc taattttagg taaatggttt tttacttcga aataacccct
     7141 cttcatttcg ttaatataag gtgtttttat caagtggata attaaaacta tatactagtt
     7201 tttcattgtt ttgtgcatta tgatcctacg actttttcag catcaaacga agttttaaat
     7261 tggcaaaaaa cataaaaaaa cagactatcc ctatcagata gtctgctaaa cgttgagtta
     7321 tcagcgtttt tattattccg cagcgtttgc gtctttgaga ccgtattttt tgttgaaacg
     7381 gtccacacgt ccgtctgctt gtgtgaattt ttgacgtcca gtgtagaatg gatgtgagtc
     7441 agaagttact tcgacacgga tgactgggta tgtattaccg tcttcccatt caactgtttc
     7501 ttgtgaagtt tttgttgaac ctgacaagaa tttgaaacca gttgttgaat ccatgaatac
     7561 tactgggtgg taatctggat ggatgttttc tttcatgatt ttttcgctcc tttgccctgc
     7621 tccatttgct gaaccagagt tgatttgtcg atttacaaca ttaacatctt aacatgtttc
     7681 agtcaaggtt gcaattatct tttcagattt ctggtcggat tttttttacc aaaggagaca
     7741 tcttgaaatt ctttgaaaaa ttgctgattg ttttttgttt tacgcaaaaa gcggatcagt
     7801 tgttccgtat attctaatga atctccagtc atattttttc gaagtttcca aatttcttct
     7861 aattcttcac ttggcatcaa taattcttct tttcgggtac ctgatttttt gatatcgatg
     7921 gctgggaaga tccgacgttc agataattca cgtgaaagtt gtaattctag atttccagtt
     7981 cctttgaatt cttcatagat cacgtcatcc atacggcttc ctgtatcgac taaagcggtc
     8041 gctaaaatcg tcaaactgcc accttcttcg atgtttcttg ctgccccgaa aaatttctta
     8101 ggtttataca atgcggctgg gtcaatcccg ccactcaatg tacgtccgct tggtggaacg
     8161 actaagttat aggcccgagc aagtctagta atactatcca tcaagataac gacatctcgt
     8221 ttatcttcga ctaaacgcat tgcgcgttcc aatactaatt cagaaactcg agtatggttt
     8281 tgcggctgca gatcaaatgt agaagatacc acgtcgcctt tcacgcttcg ttctagatcc
     8341 gttacttctt ccggtcgttc atcaatcagt agaacgatta attctacttc cggatagttt
     8401 tcggtgatac catttgcgat ttcttttaat acagaagttt tccctgcttt tggcggtgcg
     8461 acgatcaatc cacgctgacc aaaaccgatt ggagaaaaga tatcgatcat tcgggtagat
     8521 agtctttgcg gtgttgtttc cagttgaagc tgtctttgcg gataaagagg tgttaaggct
     8581 ggaaaatgcg gacgttcttt tgcttcttcc ggatcttttc cattaacgct ttccacgtgc
     8641 atcaaaccat aataacgttc cgattcttta ggtggacggg cttttcctgc cactttgtcc
     8701 ccatttctca aactgaatcg tctgatttgt gaggcagaaa tatagatatc ttctgcactt
     8761 ggtccataat tgattggccg taaaaaaccg taaccatctt gaccaacgat atccaatatt
     8821 ccttccatat aataaaatcc ttgtttctct gcttgtgcac gaatcacagc tagagataat
     8881 tcctttttat tcatcttact gtaataagga attttaaatt ctttggcata actataaact
     8941 tcttttaacg ttttgttctc tagttctgcc attgtcaaat aatcgctcat tcagctccca
     9001 cttcctctat catttcaatt gatgccccta aggctgttag tttctcgata atatgatcat
     9061 aaccgcgtaa gatataatct acattgtaaa tcgtcgttgt cccttctgcc aatagtccag
     9121 caatcactaa acaagcacct gcccgaagat cagaagccac gacttctgtg ccgtgaagtt
     9181 tatttggacc attcacgatg atcatatttc cttccacggt aatatcagcg cccattcgtg
     9241 ctagttccgg cacatggttc acacgtttcg catagatcgt atcgatgatt tctgaagtgc
     9301 ctgtagtcat caatagtaac gcagtaagtg gctgctgcag atctgtggca aatcctggat
     9361 aaggataggt catgatgttt gcgggtttta attctttcga ggggtaaact tcgatttcat
     9421 cttcgctgat tttcatatgg acaccaattt cctgcaattt tgctataaag ctttccaaat
     9481 gttcaaagat cacatttttc actttgatac catttcctac tgcagctgct aatgctagat
     9541 aggttcctgc ttcgatgcgg tcaggaatca tgaaatgccg acagccatgt aatgtttcga
     9601 ctccttcgat acggatcaca tctgtacccg ctcctcgaac ttttgccccc atattattca
     9661 gtaaagttgc cacatctatg atttccggtt ctcgtgcagc gttttcaatt actgtctgtc
     9721 cttttgcttt tacagcagct aacatcacat tgatcgttgc tccgactgaa accacgtcca
     9781 tgaaaatccg attgccacgc aatcctttat tttctgtccg taaatacatg gcaccatgtt
     9841 catttgtcac ttcagcacct agagcttcaa atcctttgac gtgtaggtca atcggtctag
     9901 ggcctaaata acagccacct ggcaggccta ctaccgcttc gccaaattta ccaagcagtg
     9961 ttcccataaa ataataagaa gcacgcaaac tattgatttt tccgtgcggc atcggaatcg
    10021 atacgacttc tcgaggatcg atttctagta tgttgttgga aaaatggaca gttgcgccca
    10081 tgatctctaa aatttcaatt aatgaatgaa catcttgtat gtccggcaca ccttccaaga
    10141 tcactgggga atcagctaag attgcagctg gaatcaatgc gactgcacta ttttttgcac
    10201 cgctgatcgt tacttcacca gctaaaggtc ggtttccttt gattacgatt tttttcatct
    10261 gtttccacct tgtttattaa tcttttttca tggatatgct tgtattttat catagataaa
    10321 aaaaagaaac gagcatggtc tgccgtttta agtaaaaaag aatggagagc gacttcaaaa
    10381 gtcgttctcc attcctatat atcgtatctg ttatgtggat gcttacgctt tgttagctga
    10441 accgaaccag tcgatatgag atttagcatc ttcaacgatt gctgctttac ctggagctaa
    10501 taatttacga gggtcgaatc ctttaccttc tttatcttta ccagcttcga tatatttacg
    10561 agtagctgca gcaaatgata attggaattc agtgttaaca tttacttttg agatacccat
    10621 tgagatagct ttttcgattt gatcttgagg aatacctgat ccgccatgta atactaaagg
    10681 catgtctgga cctacagcat cagcaattgc ttgtaagtgg tcaaaagcta atcctttcca
    10741 gttttctgga tattgaccat ggatgttacc gataccgcaa gctaagtagt cgatacctgt
    10801 agcaaccatt tgtttgcatt cttcaatatc agctaattcg ccgttaccga tgattccgtc
    10861 ttcttctccg ccgattgaac caacttcaca ttcaactgag ataccttttg catgtgcttt
    10921 ttcaacaacg tctttagcta atttcaagtt ttcttcaaat ggtagatgtg aaccatcgaa
    10981 cattactgaa gtatagccta attcgataca ttccaaagca gcttcgtaat caccatggtc
    11041 taagtggata gcaactggaa cagtgatgtt cattgcttca actaaatcag tgattaaatc
    11101 tttacagatt ttgtaaccac ccatgtattt agctgcaccc attgatgttt ggataagtac
    11161 tggtgcttgt tttgcttcag ccgcttcaag gattgcttga gtccattcca agttgtttgt
    11221 gttgaatccg ccgacagcgt aaccgccttt acgagcagct tttaaaaatt cagctcctga
    11281 tactactggc ataataaaat tcctcctaag ttttgtaaaa ctaaatttgt taagacaatt
    11341 cttaaccaac gaccctattt taccaaacat ttcggtagtt ttctactaaa atcaatgaaa
    11401 atgaaaattt tcaagaagtt ttttgcaaaa aataaaatat ccaatagcgc tttaatcgaa
    11461 aaaagtcttt tgctttctat cttttttcct acaaataata acaaatcaga aaaagagaaa
    11521 agtgacaaga aaattgctta tccccttctc ttgctacttt tctctttcta tcaagttcaa
    11581 aaattatttt gtttcgtgat ctgctaatga tgctccgaca aatgctttga ttaatttttg
    11641 cgggcgattt ggacgagaaa tcaattctgg gtggaattgg catcccacaa agaatttttt
    11701 ctctggcagc tctacgattt ctactaaacg gttatctggt gagacacctg aaaagactag
    11761 cccttgttct tcaaacaatt gacgatattt gttattgaac tcatagcggt gacggtgacg
    11821 ttcttgcaca acatcggctc catcatatgc ttcagccgtc ttcgttcctt ttttgatttt
    11881 acatgggtat aatccaaggc gtaaagttcc acccaaattc tcgatgtttt cctgatcagc
    11941 cattaaatca ataatattgt ttgctgtatc tggattcgtt tcagctgatg acgcatcttc
    12001 taatccaacg acgttacgtg caaattcgac acaagccatc tgcataccaa gacaaatacc
    12061 taaaaacgga acgtcatttt cgcgagcata acgaatcgct tcgattttcc cttctactcc
    12121 gcgatcacca aatccaccag gaaccaagat tccatctgcg tctttcaaac gttcttctac
    12181 attttctctc gttaattctt gtgctttgat ccaatcgatc tcgatgtctg aattataggc
    12241 gaaaccagaa tgtttcaatg cttcaactac tgagatatat gcatctggta actcgacata
    12301 tttaccgaca agcgcaatcc gtactttttt cttcaagttc aagacacgtt cttctaattc
    12361 tttccattct gtcatgtctg cttctggtgt atctagtttt aaatggtcgc aaacaatttg
    12421 atccattcct tgtgcttgta aaactaatgg gatggagtaa agtgtatcta catctggcga
    12481 ctcgataact gcttctggtt cgacatcaca aaattgtgcc aatttgtttt tcacgctttg
    12541 agaaacaggt tgctctgtac gtaccaccaa aatattcggt tggataccta aaccacgtag
    12601 ctcttttaca ctgtgctgcg ttggtttagt tttcatttct ccagcagctt tcaagtaagg
    12661 aatcaatgtt gtatggatat acataacatt atctgtaccg acatctgctt tcatttgacg
    12721 aagtgcttca aggaatggca aagattctat atcccctact gttcccccta cttctgtaat
    12781 gatcacatct gaatcagtca ttttagctgc tcgcatgatt ttttctttga tctcatttgt
    12841 aatatgagga ataacttgaa cagttgctcc caagtattcg cccttacgtt ctttacgcaa
    12901 aacttcagaa tagatttttc ctgtcgtcac gttggagtat ttgttcaaat tgatgtcaat
    12961 aaaacgttcg tagtggccca gatccaaatc tgtttcagca ccatcatctg ttacgaatac
    13021 ttctccgtgc tgatatgggc tcatcgtccc cggatccacg ttgatgtatg gatcaaattt
    13081 ttggatagtt acttttaagc cgcggttttt caataagcgt cctaaagaag ctgcaacaat
    13141 cccttttccg attgatgaaa cgacaccgcc ggtcacaaaa atatattttg tcataagttc
    13201 aaaaactcct ttttaattta tgtgcaggaa aagtctcaga caataaacaa agctccctat
    13261 tcttcaataa gaatagggag ccgaaatttc tgtaaacttc tgaccctcta aaagggtgcc
    13321 caaggtgtat aatacaagct gactaaggga aagtcaagta ctcaaatgaa tactatccgc
    13381 ttacaatttt ttctttttcg atatcctttg agaaattatt tcttaagtcc tttatgagtg
    13441 caaaaaaaat ttaacccaca taataagctt gatataaaca tagctaacgt taaacctaag
    13501 caaatcagaa aatcgttact gaaaaatttt aagtgaaata atagataaaa aaataaaaca
    13561 aacattatga tagataatat cccacctaac aaagaactac tttttcctac ttgcagcaac
    13621 agtttatata gcttatcgta gaaacaatct ttttctttca cccttattta cctcctttac
    13681 gtaaaaaacg tagtccacag ttctcggatt tttggcatat cttttctatt aaataatatc
    13741 aaaaacaaaa caattccact aattttttca aagatgacct ttttttcaac aaaagtgcct
    13801 tgtagggatt cattgaaggt aacgaatcac ataagtaaaa aataaggaga tgcgacaaaa
    13861 gtcgtgcctc cttatttcta aataaacggt gttctcctgc caactcctcg gtcacaagtc
    13921 acaatatcca tcaaacatga gaagctgttt ttgaaattat ttcttgtgat tcggagttaa
    13981 atggtaggct cacaacctct ggataaacgg tattcaaaag ccagtttctc ggtattaagc
    14041 caaatttatg caaaaatgta gaaagccatt ttcacaaaat ttttcttaat actcgaaact
    14101 aaacgtcttt ttcaaaacct cttggtaaac ggtgttcaaa agcttgcatc tgcggtaata
    14161 agttcagcca attcaaaaac aagaaaagct gtttttgctt ggctattctt attacttgtt
    14221 gcagggcagc tttttcacaa cctctccact aaacggtgtt caatcagctg accctcggta
    14281 ttagctcaaa aaacggcaaa atatgaggaa ctatttttcc gttagtttct ctaatactca
    14341 ggtcaaaacg ctgatttcac agcctctaat tcacggtatt cctgttttaa taaagttcta
    14401 gatattgttc tctttcccac tcggaaacag tttgtctgaa tgccgcccat tcgagacgtt
    14461 ttgcttcaac aaaatttgag aagatatgtt gtcctaatgc gtcaatcatt acattatcct
    14521 tacgcaattc tttgatagca ttatggatag ttgaaggtaa gtcctggata tgtgcagctt
    14581 ctctttcttc ctcatccatt acataaatgt tacggtcaac agctgctggc ggtgtgatct
    14641 gattacggat accgtctagt cctgcttgca acaatactgc cattgccaag tatgggtttg
    14701 cagatggatc aactgaccgc aattccaaac gtgtggataa accgcgagat tctggtacac
    14761 gtactaatgg cgaacgatta cggccactcc atgcgacata aacaggcgct tcataacctg
    14821 gaacgagacg tttataagaa ttgactgttg gattgcaaac ggctgtgtag gcacgagcat
    14881 gtttcagcaa accacctaag aaatgataag ctgtttcact tagttccaat tcaccgtctt
    14941 tatcatagaa aacatttcct tcatcattga acaaagacat attgcaatgc ataccagaac
    15001 cattgatccc aaataacggt ttcggcatga aagtcgcgtg aagtccatgc ttgcgtgcaa
    15061 tcgttttgac aacgagcttg aaggtttgga tattatcaca agcatctacc acatctgcat
    15121 atttaaagtc aatttcatgc tgtcctggcg ctacttcatg atgtgaagct tccacttcaa
    15181 agcctagact ttctaattcc aacacgatgt cacggcggca attttcacct aaatctgttg
    15241 gtgcaaaatc aaaataacca cctttatcat tcaaggtcgt tgtgatccca ccgtcttcgt
    15301 ctaatttaaa taagaagaat tctggttcag gtcctaaatt gaaagaggta aaaccaaggt
    15361 cgcgcatatc tttcaatgct cgtttcaaat tcccgcgagg atcgccggca aatggtgtcc
    15421 catccggatt atagatatca caaattaaac gagcaacttt tccatgagca ctttcccacg
    15481 ggaagatcat ccaagtggag agatctggat acaaatacat atcgctttct tcaattcgaa
    15541 caaatccttc aatcgaagaa ccgtcaaaca tcattttatt ttctaatact ttgtcgatct
    15601 ggctcactgg aacttctaca tttttgatcg tacctaagat gtctgtgaac atcaatcgta
    15661 aaaatcgtac gttttcttcc tcaatgattc gtttgatttc ttcacctgtt atctgtttct
    15721 ttaccatggt atccctcttc cttttttaat ttattcagtc aaagaattgt ctttgcaatc
    15781 cttgcgactg ataatgacct gttttgctta acccaccttg cgccaaaatc tcatcacgaa
    15841 aaatttgtcg aacgtcatga tctgtcaacg gcttcttgtt ttttgcttct tgttttttcg
    15901 cctgtgccat ttcgtataca cgtttgatcc cagccatatt caatccctct gaaagatagt
    15961 ctttaatctc aagcaacata tcaatatcat tcagcgaata catacgtcgg tttccttcgc
    16021 ttcgttccgg atgaactaaa tcttgttctt cataatagcg aatttgccga gcagacaaat
    16081 cagtcagctt cataactgta ccaattggaa atacggacat agatcggcgc aattcctttt
    16141 ctcccatcag acgtcccctt tcgttttata agttggctaa agtgtaccaa ttaaaaaaaa
    16201 tattgtcaac tgcatatgtt aggttttcta acatgagatc tctatttttt ataaaataac
    16261 aaaaaaataa gaacacaaaa aaggtatcta gttgattttc gtaccagata ccttttattt
    16321 tttattatac tgtactatcc tttattagtg tttttatt
//
LOCUS       DACCDF010000060        16075 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCDF010000060 DACCDF010000000
VERSION     DACCDF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.063"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IVK51_001968"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IVK51_001968"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IVK51_001969"
     CDS             complement(227..697)
                     /locus_tag="IVK51_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP6431549.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IVK51_001970"
     CDS             complement(856..1314)
                     /locus_tag="IVK51_001970"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP6431550.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IVK51_001971"
     CDS             complement(1485..2630)
                     /locus_tag="IVK51_001971"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP6431551.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IVK51_001972"
     CDS             complement(2643..3560)
                     /locus_tag="IVK51_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP6431552.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPNGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IVK51_001973"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IVK51_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP6431553.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IVK51_001974"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IVK51_001974"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP6431554.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IVK51_001975"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IVK51_001975"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP6431555.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IVK51_001976"
     CDS             7086..7967
                     /locus_tag="IVK51_001976"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP6431556.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IVK51_001977"
     CDS             7964..8680
                     /locus_tag="IVK51_001977"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP6431557.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IVK51_001978"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IVK51_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP6431558.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IVK51_001979"
     CDS             9397..10398
                     /locus_tag="IVK51_001979"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP6431559.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IVK51_001980"
     CDS             10395..11756
                     /locus_tag="IVK51_001980"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP6431560.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IVK51_001981"
     CDS             11716..12867
                     /locus_tag="IVK51_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP6431561.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IVK51_001982"
     CDS             12842..14011
                     /locus_tag="IVK51_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6431562.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..15565
                     /locus_tag="IVK51_001983"
     CDS             14186..15565
                     /locus_tag="IVK51_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6431563.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15846..16075
                     /locus_tag="IVK51_001984"
                     /pseudo
     CDS             15846..16075
                     /locus_tag="IVK51_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5257 a         2839 c         3428 g         4551 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacca
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atttgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg ataaaaaaac
    14461 agtaacggtt actagcgcat cagataacga aacaaaagaa tatccttatg acaaattgat
    14521 tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc tagaaaatgt
    14581 ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa atgatccggc
    14641 tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag cagaagcaag
    14701 ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt taggcactta
    14761 tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg gtattgaagt
    14821 tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg caatcaaaac
    14881 aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga aggcaaatac
    14941 agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa caaatgaata
    15001 cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg cttattctat
    15061 cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag aagcacgtta
    15121 tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag tcgtaggttc
    15181 ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata gctttacagc
    15241 agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac gaccaaaata
    15301 tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg atccaatgac
    15361 ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc aaggaaatgt
    15421 attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag ctgacttctt
    15481 cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc aacaagcgtt
    15541 aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt tgtgaaaaag
    15601 ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg tttttaactt
    15661 ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca aaaaagggac
    15721 tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa cttttggagg
    15781 tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt tcatgattat
    15841 ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa gcggaaatgc
    15901 ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat gaactaagaa
    15961 accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag ctaggaataa
    16021 aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga aaagt
//
LOCUS       DACCDF010000061        15802 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCDF010000061 DACCDF010000000
VERSION     DACCDF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.064"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            68..751
                     /locus_tag="IVK51_001985"
     CDS             68..751
                     /locus_tag="IVK51_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP6431564.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IVK51_001986"
     CDS             753..1589
                     /locus_tag="IVK51_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP6431565.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IVK51_001987"
     CDS             1600..3354
                     /locus_tag="IVK51_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431566.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IVK51_001988"
     CDS             complement(3630..3971)
                     /locus_tag="IVK51_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP6431567.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IVK51_001989"
     CDS             complement(4039..6210)
                     /locus_tag="IVK51_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP6431568.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IVK51_001990"
     CDS             6421..7278
                     /locus_tag="IVK51_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP6431569.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IVK51_001991"
     CDS             complement(7349..8206)
                     /locus_tag="IVK51_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6431570.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IVK51_001992"
     CDS             complement(8191..10893)
                     /locus_tag="IVK51_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6431571.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IVK51_001993"
     CDS             complement(10906..12195)
                     /locus_tag="IVK51_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP6431572.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IVK51_001994"
     CDS             12345..13391
                     /locus_tag="IVK51_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6431573.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IVK51_001995"
     CDS             13393..15546
                     /locus_tag="IVK51_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP6431574.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4486 a         3302 c         2915 g         5099 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gt
//
LOCUS       DACCDF010000062        15736 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCDF010000062 DACCDF010000000
VERSION     DACCDF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.065"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(24..722)
                     /locus_tag="IVK51_001996"
     CDS             complement(24..722)
                     /locus_tag="IVK51_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP6431575.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="IVK51_001997"
     CDS             complement(715..2067)
                     /locus_tag="IVK51_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP6431576.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="IVK51_001998"
     CDS             complement(2290..3690)
                     /locus_tag="IVK51_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP6431577.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="IVK51_001999"
     CDS             3851..4588
                     /locus_tag="IVK51_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP6431578.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="IVK51_002000"
     CDS             4713..5192
                     /locus_tag="IVK51_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP6431579.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="IVK51_002001"
     CDS             5189..5956
                     /locus_tag="IVK51_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431580.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="IVK51_002002"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="IVK51_002002"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6431581.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="IVK51_002003"
     CDS             complement(6859..7461)
                     /locus_tag="IVK51_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6431582.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="IVK51_002004"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="IVK51_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="IVK51_002005"
     CDS             8142..8726
                     /locus_tag="IVK51_002005"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP6431583.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="IVK51_002006"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="IVK51_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP6431584.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="IVK51_002007"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="IVK51_002007"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP6431585.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="IVK51_002008"
     CDS             10627..10806
                     /locus_tag="IVK51_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431586.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="IVK51_002009"
     CDS             10828..11853
                     /locus_tag="IVK51_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP6431587.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="IVK51_002010"
     CDS             12028..13272
                     /locus_tag="IVK51_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP6431588.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="IVK51_002011"
     CDS             13425..14372
                     /locus_tag="IVK51_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6431589.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="IVK51_002012"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="IVK51_002012"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP6431590.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="IVK51_002013"
     CDS             complement(15274..15516)
                     /locus_tag="IVK51_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431591.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="IVK51_002014"
     tRNA            15637..15709
                     /locus_tag="IVK51_002014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
BASE COUNT         5348 a         2823 c         3195 g         4370 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaagg
//
LOCUS       DACCDF010000063        15513 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCDF010000063 DACCDF010000000
VERSION     DACCDF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.066"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(330..632)
                     /locus_tag="IVK51_002015"
     CDS             complement(330..632)
                     /locus_tag="IVK51_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431592.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="IVK51_002016"
     tmRNA           complement(852..1218)
                     /gene="ssrA"
                     /locus_tag="IVK51_002016"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1389..2162)
                     /locus_tag="IVK51_002017"
     CDS             complement(1389..2162)
                     /locus_tag="IVK51_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6431593.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2306..2656
                     /locus_tag="IVK51_002018"
     CDS             2306..2656
                     /locus_tag="IVK51_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6431594.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2637..3353
                     /locus_tag="IVK51_002019"
     CDS             2637..3353
                     /locus_tag="IVK51_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431595.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3353..4801
                     /locus_tag="IVK51_002020"
     CDS             3353..4801
                     /locus_tag="IVK51_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6431596.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4871..5380)
                     /locus_tag="IVK51_002021"
     CDS             complement(4871..5380)
                     /locus_tag="IVK51_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431597.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5370..6095)
                     /locus_tag="IVK51_002022"
     CDS             complement(5370..6095)
                     /locus_tag="IVK51_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431598.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6318..8438)
                     /locus_tag="IVK51_002023"
     CDS             complement(6318..8438)
                     /locus_tag="IVK51_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6431599.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8668..9846
                     /locus_tag="IVK51_002024"
     CDS             8668..9846
                     /locus_tag="IVK51_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP6431600.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9862..10620
                     /gene="aroD"
                     /locus_tag="IVK51_002025"
     CDS             9862..10620
                     /gene="aroD"
                     /locus_tag="IVK51_002025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP6431601.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10643..11128
                     /gene="ybaK"
                     /locus_tag="IVK51_002026"
     CDS             10643..11128
                     /gene="ybaK"
                     /locus_tag="IVK51_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP6431602.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11199..12452)
                     /locus_tag="IVK51_002027"
     CDS             complement(11199..12452)
                     /locus_tag="IVK51_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6431603.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12569..13918
                     /gene="gdhA"
                     /locus_tag="IVK51_002028"
     CDS             12569..13918
                     /gene="gdhA"
                     /locus_tag="IVK51_002028"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP6431604.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14030..15376
                     /locus_tag="IVK51_002029"
     CDS             14030..15376
                     /locus_tag="IVK51_002029"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP6431605.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
BASE COUNT         5126 a         2750 c         3124 g         4513 t
ORIGIN      
        1 aaagacattt gggttatatt gtgagataat aataaagaag agtttaaagc gttccccaaa
       61 aaccacttcc ccataagtgt gttacgcttt aaactctttt atatttgaag ccattaaaaa
      121 gcataccata taactgtaaa aaataatggg aaaaagactt ataattggag tggtagttaa
      181 ttagtgactt atttttgatt ttatagcact gatactataa aatatagata tcatcatatt
      241 acacaatctt aatactaact taaaaaatat cccttttata agtatggtga taaaatccgt
      301 tccgggctac cttttttagg tagcctactt taatctttat acctttctgg atcaacgaaa
      361 gtatacttca aatagtcata acgccgatga tcgctccgtg cgtctggcac atcagtcacg
      421 atatcaaaca aaaaatacac atctttcttc attctggttt ttgttgccgg aattttaaag
      481 tagttcttat tagagtaata gagattgatt aataagctat cttcgattgc taaaaagaaa
      541 acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg atcaaattta
      601 aaccataatt gtgtttttcc ttccatcaac ataatgttca cctcaaaaag agtatacgaa
      661 ctaatgttct ttttgtaaag taggaatatc attgataata tggatggaac atatatatta
      721 aaaagtgttc tttttgaatg ctattttgaa tgccattttc attcatttaa cgaaaatctg
      781 cgaaaataaa aaatgctcaa acccttataa aataaggatt taagcatcta gcgaaatctg
      841 ctgaaatctc acaatggaga cggcgggagt cgaacccgcg tccaaacaca tcgccactta
      901 aaaatctacg ttcatagtca actcatttaa ggattcactt tacagcttgc cgagtgacag
      961 gcattctgta tcgctagtct gatgttctct ttttcaactt acagacggaa agttgaaacg
     1021 tatcccacta aagttaggac ccttactcga gcacatgggc gatgccgaga ggatctacgc
     1081 taactgtttt taggcagcta aagcgtaaga gttgttttcg tttttagcag ttatatttaa
     1141 ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat tcaagctcga ctgtgcctgt
     1201 cgaatccgta acgtccccgt tagctaacaa gggttacgtg tatttttcat acaaaattag
     1261 tatagcatat attgggataa attcaataaa aaagcgaatc atatcccatt atttatataa
     1321 taactgcccc tttcttaaga aaaaataagc gattttctta agaaaagagg tagcttcaaa
     1381 aaaagatctt aaatcagatc aaaatacttc aatgcgtgga caattccgcc ttctgtattt
     1441 ttctttgtga taaaggtaga aagttctttt aatccgtcat aggcgttgcc catagcgatt
     1501 ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac catcgccaaa tccaaaagta
     1561 ggaacttttt ccagctgaag ttctttttgc agacgtttga cacctgcccc ttttgagatt
     1621 cctttcttca ctgtatcgat cgagtaagga ccattccgat aaaaagttag ctcaggaaaa
     1681 tgttcgtgat agtattcgtc acctgattga gataagatca gcaacatatt tactgtgttt
     1741 tctttaaagc ctaaaggatc aatttccgga acatctgaat gaatgaagtc gtaagcattg
     1801 atcattgttt ggctatgacc ggtacaccag attttctgat cgttatagta agaaagttca
     1861 tgaccgttca actgtacatg atcatacatt ttttgacatt cttctgaaga aaatgtttga
     1921 gaatagattt cttcgccgtc tactcgaatg aaagaaccat tcatggtgac cgcagaagta
     1981 atgcccgcat cttccatgat agagtggatt tctgcttctg tacgtcctgt agcgatgacg
     2041 gggagaacat tgttttcttt taatgcgttc atagcttttg ccacttcggg tgtgattttt
     2101 gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag tgattgcacg ataatttgac
     2161 acaaataaaa ctcctttttt attggaataa gtttatctta acaaagaatg aacaaaagac
     2221 aaacctttcg tgaagaattc ctgaacatat tggttcagtc aacaatttag agtatactat
     2281 aagaagagaa tttaggaggg aaagaatgat ttttttcagt attggtatct tattcttaat
     2341 aattggcagt ctttttcgga ttctgccctc aaaaggaaat ctgccttttt atgggtatca
     2401 ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg gctcaaaaga caagcggaaa
     2461 ctggtttttt cttatgggct tactgatggc attgatcggc tattatttaa agacatctgg
     2521 gcatacgaat tattttctga ttgaaatgct tctgctagta tttccaatta tgcctatttt
     2581 tattatgaca gaaaagaaat tacagaaata tgacctggaa actggaggga acgataatga
     2641 atattttaat gattgaagat aatcaatctg tttctgaaat gatgcagatg tttttcttaa
     2701 atgaaggttg ggaagcaact ttcaagtatg atggaaaaga agggctcgat gcctttttag
     2761 aaaacccaca acattgggat atgattacac tagacctgaa tttgcccaca ctggacggta
     2821 tgctagtagg aagagagatc cgcaaagttt caagtactgt gccgattatc atgctgacag
     2881 ctagagattc tgaaagcgat caggtgatcg gcttagagat gggggcagat gattatgtca
     2941 caaaaccttt cagtcctctg acgcttattg ctcgaatgaa agctttgcat cgccgtagtg
     3001 agttggcaga agcaagagaa gaagcagtag tttcagatga tcattttgat atcgagacta
     3061 gtcatttcaa gatgaatacg aaaacacgag aagcgtatct ggacaaccgg ccaattgaag
     3121 gactgacgcc taaagagttc gatctattgt ttactttagc aaaaaaacca cgtcaagtat
     3181 tctctcggga gcagttgctt gaattagtct gggattatca gtactttggt gatgaacgga
     3241 cagtggacgc ccatatcaag aaattacgac aaaaaatcga aaaggtcgga ccacaagtca
     3301 tccaaactgt ttggggagtc ggctacaaat tcgatgattc tggtgttgcc taatgcgtta
     3361 tttgtatcag caactcttag ctttctgcgg aatggtcgcg ttgatcttat tgatcgttgg
     3421 agtttctttt acacaactga caaaacaaac gattgaagaa aacaattacc agcaactgtt
     3481 tggatatgct gaatcagttg aaaagacaac gcaaacctat gccgatacgc tcccgcagtt
     3541 tagtcaagat caggcgtttc aaaatgcatt gctgctgaca gagcaagtac tgacagaaca
     3601 aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc atttatccga ctaatacagc
     3661 tttactgaat tttaccttaa cagctgatca atggaaaaat ctgaggaatg gacgtcaaga
     3721 aaaattcaca tcgaataaaa atattttagg acaagcacag gcgacctctt acgcacttgt
     3781 gccatttaat ttgaatcatg aattctatgg agcacttgta gttagtcaac ctgcgcgaaa
     3841 tatcgataac agcgttcgtc caattacact gaacctgttc aaaggattta ttttttccag
     3901 tatcgttgca gttattgcca gctatgcttt tgcagcattc caagttaagc gtatcaatcg
     3961 gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt gatgtacagc tccccgtaca
     4021 tgacaaagat gaatttgatg agttagcaga tgatttcaat aaaatgacga attcattgaa
     4081 agaatctcaa gcagaaattg aagaacaaga aaatcgtcga agacaattta tggcagatgc
     4141 ttctcacgag atgagaacac ctcttacgac aatcaacgga cttctggaag ggttagaata
     4201 caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa ttgatgaaaa atgaaacaga
     4261 gcgtctgatc cgtttggtga atgaaaactt ggattatgaa aaaatccgta cgaatcaaat
     4321 cagtatggtc attaaaaaat tcaatggaac agaaacattg aaaaatattg ttgcacagtt
     4381 agaagctaaa gcagaagcag caggagatac attgacattg aaagccgttg atgatattga
     4441 tgtttacgct gattatgacc gatttgtcca aatcatggtc aatattattc aaaacgcgat
     4501 acagtttact gaaaacggcc aaatcacgat tactttagaa aaaggctatc ttgaaacgat
     4561 catcacgatt gaagatacgg gaattggaat gtctgaacaa caaatgaaaa gtatctggga
     4621 tcgttattat aaagtagatc cttcaagaaa aaatacaaaa tatggtgaat caggattagg
     4681 tttaccaatc gtgcagcaac ttgtacgctt gcataaagga aaattagaag tagaaagcga
     4741 actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac gtagaaatca aaaaagagta
     4801 aaaaacaagc ggtataaaag atcatgaatc gatcttctat accgcttgtt tttactataa
     4861 aatgattgat ttagcccgcg ggctctaaac gatcaatata aggactttga gcaatagtag
     4921 catcatgagt aaagagtaag ataccttgat tttcttcatg agctaaataa gaaagcaagt
     4981 ggagtagctc ttggatctcc aatacactaa gttcatcaaa tgtatcttca ataacaatat
     5041 tgtttcgctt ggaaagcaag gcatgaagta attgcagctt tactttttca aaagacgtca
     5101 actcagctgc cgcacaatta agcaaggaag gagagatatc aagtttttct tgataggcca
     5161 aacgatcttg tttgatttct ttttccggta catccaacag gacatggtct ttaagattca
     5221 gataaggaaa catcggccaa tttttttgga tgatcgcaaa attatccagt tttgcaacaa
     5281 gaacctcttt tttcaagtgc tccaatattt tatgctgttt ttcgagagga cacaaaataa
     5341 cggtcaagtg ttcagcatga atcaaatatt caggtttcat tacgtccagg cctcctactt
     5401 tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa gaatgagaca gcatccactg
     5461 agcccaataa gagtttgggc aaatccctta agtatatctg tgaaaaaaag atcattttgg
     5521 aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg taagtccgct taccgaagtc
     5581 aagatatcac ggatatctgg cagttcagta gtctttaaga caagaaaatt gatcctttga
     5641 agaagcgctt cataaactga ctggaagctt acgatccaca ataaaaccaa gatacaacaa
     5701 agaaagagag gaaagaacag ctgccagaaa ataaaaagga ataatttggt tttagaaaga
     5761 ccagtcaggt gccaattgat caattcctgc ttgttttgcc tgactgaata gtaaaagaaa
     5821 aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata tgccttttat ataaaatcgg
     5881 ctagctaatt ctaaagccgg aagaagctct gtagatgcag aagcgccaat aaatgaaagc
     5941 cgatcttgga cttgattggc taaagccgat tgcaaatggt aaagagtcag gcatgctatc
     6001 aataaaaaag cagaaaagct tgaaaataaa caatagagta ttgtctgctt tttgtaataa
     6061 attaatcgag cagtagcata gcggattatt ttcatacttt tcacctctat aaaaagtata
     6121 gaaaatgaat atgaataaag tatgaaattc ctttagtgaa atcacgaatt aaaaccagaa
     6181 aatattttgt aaaaagcaag aggttgtgaa gaagctgccc tattctatta ccgtagatgc
     6241 aagcttttga acactgttta ttcgaaaaga ggagcagcga caataatttt gtcgctgctc
     6301 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
     6361 gtttgcgttt tataaagatc aacagttgat tgtccgttgt gttcactaaa gacactagtt
     6421 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
     6481 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
     6541 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
     6601 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
     6661 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
     6721 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
     6781 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
     6841 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
     6901 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
     6961 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
     7021 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
     7081 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
     7141 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
     7201 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     7261 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     7321 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     7381 ctaatgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     7441 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     7501 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     7561 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt catgctggag accattctca
     7621 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     7681 ttttttcctc tagcgagccc gaacaaatcg tcatttggtg ctgcataatg ttcttttaca
     7741 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     7801 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     7861 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     7921 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     7981 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     8041 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     8101 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     8161 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     8221 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     8281 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     8341 aaataggcaa aaatcgtttt caaccaaagt aaaacgacta gtaaagaaaa aaatccgagc
     8401 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     8461 cttaatcaca gaagtgtcct gaaaccatgt tacacagctc acttattctc atttatttta
     8521 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     8581 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     8641 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     8701 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     8761 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     8821 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     8881 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     8941 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     9001 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     9061 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcatagg aggctatgag
     9121 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     9181 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     9241 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     9301 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     9361 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     9421 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     9481 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     9541 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     9601 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     9661 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     9721 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     9781 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     9841 cattagaagg agcgatgcag aatggttcgg gtaaagggat tgaatatagg aagcggtaga
     9901 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     9961 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
    10021 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
    10081 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
    10141 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
    10201 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
    10261 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
    10321 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
    10381 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
    10441 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggttggc
    10501 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
    10561 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
    10621 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
    10681 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
    10741 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcgta tatttaaaac
    10801 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
    10861 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
    10921 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
    10981 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
    11041 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
    11101 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
    11161 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
    11221 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
    11281 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
    11341 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
    11401 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
    11461 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
    11521 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
    11581 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
    11641 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
    11701 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
    11761 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
    11821 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
    11881 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
    11941 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
    12001 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
    12061 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
    12121 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
    12181 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
    12241 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
    12301 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
    12361 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
    12421 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
    12481 tctgaagaaa gaaaacagaa aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
    12541 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
    12601 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
    12661 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
    12721 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
    12781 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
    12841 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
    12901 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
    12961 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
    13021 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
    13081 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
    13141 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
    13201 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
    13261 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
    13321 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
    13381 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
    13441 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
    13501 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
    13561 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
    13621 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
    13681 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgtccagcg
    13741 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
    13801 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
    13861 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
    13921 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
    13981 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
    14041 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
    14101 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
    14161 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
    14221 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
    14281 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
    14341 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
    14401 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
    14461 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
    14521 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
    14581 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
    14641 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
    14701 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
    14761 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
    14821 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
    14881 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
    14941 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
    15001 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
    15061 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
    15121 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
    15181 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
    15241 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
    15301 caagaaaaac atgttcgcac ttctaggcaa accaggcttt gaagatcttg ctaaagaatt
    15361 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
    15421 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
    15481 ttcagaataa ggggctgtga caagacgttt gtc
//
LOCUS       DACCDF010000064        15347 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCDF010000064 DACCDF010000000
VERSION     DACCDF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.067"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..563)
                     /locus_tag="IVK51_002030"
     CDS             complement(<1..563)
                     /locus_tag="IVK51_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP6431606.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IVK51_002031"
     CDS             complement(750..1400)
                     /locus_tag="IVK51_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431607.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IVK51_002032"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IVK51_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP6431608.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IVK51_002033"
     CDS             complement(2912..3910)
                     /locus_tag="IVK51_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP6431609.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IVK51_002034"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IVK51_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP6431610.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IVK51_002035"
     CDS             complement(4926..5657)
                     /locus_tag="IVK51_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP6431611.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IVK51_002036"
     CDS             complement(5661..6926)
                     /locus_tag="IVK51_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP6431612.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IVK51_002037"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IVK51_002037"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP6431613.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IVK51_002038"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IVK51_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP6431614.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IVK51_002039"
     CDS             12268..13770
                     /locus_tag="IVK51_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6431615.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IVK51_002040"
     CDS             13772..14509
                     /locus_tag="IVK51_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP6431616.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IVK51_002041"
     CDS             complement(14554..14727)
                     /locus_tag="IVK51_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6431617.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..>15347
                     /locus_tag="IVK51_002042"
     CDS             14703..>15347
                     /locus_tag="IVK51_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP6431618.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLA"
BASE COUNT         4518 a         3288 c         2591 g         4950 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggct
//
LOCUS       DACCDF010000065        14975 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCDF010000065 DACCDF010000000
VERSION     DACCDF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.068"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(65..244)
                     /gene="rpmF"
                     /locus_tag="IVK51_002043"
     CDS             complement(65..244)
                     /gene="rpmF"
                     /locus_tag="IVK51_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP6431619.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(302..871)
                     /locus_tag="IVK51_002044"
     CDS             complement(302..871)
                     /locus_tag="IVK51_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP6431620.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1057..1959
                     /locus_tag="IVK51_002045"
     CDS             1057..1959
                     /locus_tag="IVK51_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6431621.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(1994..3778)
                     /gene="pyk"
                     /locus_tag="IVK51_002046"
     CDS             complement(1994..3778)
                     /gene="pyk"
                     /locus_tag="IVK51_002046"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP6431622.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3852..4814)
                     /gene="pfkA"
                     /locus_tag="IVK51_002047"
     CDS             complement(3852..4814)
                     /gene="pfkA"
                     /locus_tag="IVK51_002047"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP6431623.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(4982..8296)
                     /gene="dnaE"
                     /locus_tag="IVK51_002048"
     CDS             complement(4982..8296)
                     /gene="dnaE"
                     /locus_tag="IVK51_002048"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP6431624.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8527..8712
                     /locus_tag="IVK51_002049"
     CDS             8527..8712
                     /locus_tag="IVK51_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP6431625.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8774..9970)
                     /locus_tag="IVK51_002050"
     CDS             complement(8774..9970)
                     /locus_tag="IVK51_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP6431626.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10140..11576)
                     /locus_tag="IVK51_002051"
     CDS             complement(10140..11576)
                     /locus_tag="IVK51_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP6431627.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVVTTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11869..12678
                     /locus_tag="IVK51_002052"
     CDS             11869..12678
                     /locus_tag="IVK51_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6431628.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12864..14861)
                     /locus_tag="IVK51_002053"
     CDS             complement(12864..14861)
                     /locus_tag="IVK51_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP6431629.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4373 a         3174 c         2659 g         4769 t
ORIGIN      
        1 aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt ctaattaaaa
       61 tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa tttgcacata
      121 cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct ttgattgtca
      181 atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta gctggtactg
      241 ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat atattccaat
      301 cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt tttgcaagac
      361 gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct tcttcggaaa
      421 caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct tttgtcaata
      481 cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa ttcaataatg
      541 gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt tggaattgtt
      601 cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca ggttctagtg
      661 aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata tagcctttct
      721 tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt aaattgtctc
      781 gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt gtctcctggt
      841 attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca acaaaagcaa
      901 ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc agttcataaa
      961 aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga atcatttaca
     1021 ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga tatcgaatac
     1081 tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt ccgtgtgagc
     1141 caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac cgtacttatt
     1201 acaagagatc gatcgcatca cgaattaatc gtcacgcctt ctggaaagca gctgtttctc
     1261 catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac tcagttgcaa
     1321 aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt tcctaaactg
     1381 tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga tggaggctct
     1441 cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt aggctcaaca
     1501 cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg tttcatgatc
     1561 gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa agatatagca
     1621 gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt cactaagctt
     1681 tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt gacgatttta
     1741 aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc tgtccatcct
     1801 gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt cttgatttca
     1861 ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt ccaagttatc
     1921 catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg tttttatttt
     1981 ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg cgacgtgaat
     2041 caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca ccaacgatta
     2101 caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg ccttcttcaa
     2161 cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt gatggtacaa
     2221 ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa gcaataactg
     2281 catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta gaaccaatca
     2341 tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca gcagtgatca
     2401 agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct gctagttcaa
     2461 acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc cagtttaaca
     2521 tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca tctggacgat
     2581 attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt gttttcacgc
     2641 ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca tctgttttat
     2701 cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct tcttggtcga
     2761 tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat tcaccagata
     2821 gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct tccgcacgtg
     2881 ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata actgggatac
     2941 cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt tcagctggga
     3001 tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg atgatttcgt
     3061 caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca tgagtcatgt
     3121 ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt acaaaacttg
     3181 cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca tcttttggag
     3241 taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt gatcctagca
     3301 tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt tcaatgattt
     3361 gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca tcgtcaaata
     3421 atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga tccatagaga
     3481 tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa tcagcttctg
     3541 ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga ataccgacat
     3601 cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat tgttcttcat
     3661 ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc aattgagaaa
     3721 taatttctac tgagttactt gctggtccta atgtactaac gattttggtt tttttcattt
     3781 aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa atagcttggt
     3841 ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa gtctggttta
     3901 tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt gtcatggata
     3961 ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc accgaattta
     4021 cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg tcccaagata
     4081 gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc atttccgccc
     4141 attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg gccttcttgg
     4201 atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat ttcatccgct
     4261 ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat tacttcgata
     4321 acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga ttctagtact
     4381 gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc attatcgatt
     4441 gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc gccatgataa
     4501 gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa ttgttcgatt
     4561 ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata caagaatgta
     4621 ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat atctccagca
     4681 actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta aataccttta
     4741 cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact ggtcaaaatt
     4801 ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa gtatgactta
     4861 ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt ttctcacttg
     4921 tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt tttaccgtaa
     4981 gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgcccc tttatactct
     5041 tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt ttttcgtgat
     5101 acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat tgtttcatta
     5161 tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa ggatactggg
     5221 cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc tgattatatt
     5281 tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac aaattacgat
     5341 atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg tcggttcctt
     5401 caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc ttgatcatca
     5461 ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact tgtttagcta
     5521 aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct aagtactcct
     5581 cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct tttaatgcca
     5641 tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt tttccttcga
     5701 gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct actgctgtca
     5761 gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg aattgatcca
     5821 ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca aaatcacgcc
     5881 gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc aactcaaaac
     5941 tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta cgtgcttctg
     6001 caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg ctggtaaaga
     6061 aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat gaataagcaa
     6121 atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca tatacggtcg
     6181 ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc gacttttctt
     6241 catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct gactgcccta
     6301 atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag acgatcactc
     6361 catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg gcttcttttc
     6421 ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga tacaatgcat
     6481 tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg ttccggatac
     6541 cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag agcagcgttt
     6601 ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact cgcttaacag
     6661 ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc attttcaaaa
     6721 gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca tcagaaccgc
     6781 gttggagcgg aactagttca agtagattct tatcgctgat aactacacct gctgcgtgag
     6841 tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa agttggtttt
     6901 tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct tgggtcaatg
     6961 tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt tgcgataaac
     7021 caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca aatgttgcga
     7081 tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact tcttcacgtc
     7141 gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg tttaaaaagc
     7201 gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac acataggcta
     7261 ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt ctatgagcaa
     7321 aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga tggatgacag
     7381 ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt cggcgttcca
     7441 cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta ggaactggat
     7501 aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca gctaaacgct
     7561 gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc atttcttgtt
     7621 ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt tctaaaggta
     7681 tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct tcttgttgta
     7741 aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca tactgatacc
     7801 attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt ctagtaaaaa
     7861 cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg aaagcctcag
     7921 aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc atttctgcaa
     7981 gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc tgataaccta
     8041 tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta taactaagat
     8101 gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat tcaggtgcac
     8161 cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt tttgcttctt
     8221 gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta attgtataaa
     8281 gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac tagcgttaaa
     8341 aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta ttccacttca
     8401 tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat tttattgaga
     8461 tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag aaaagaggtg
     8521 ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca agtagtcggt
     8581 tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac aatcatttca
     8641 ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa agaaacctct
     8701 gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt ttattttcta
     8761 ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat aaagaaagaa
     8821 gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa ttataagcat
     8881 gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta aacatctgat
     8941 tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc agtgtattga
     9001 cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata cctaattgga
     9061 gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg agtcctccaa
     9121 ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt gaagaaaaga
     9181 cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata ttttctgtac
     9241 ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg attcctatct
     9301 gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc atttttgaaa
     9361 aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct tctttgacta
     9421 agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag aatacatttt
     9481 ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga tttccagcaa
     9541 tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt gcctgtgaag
     9601 caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa aaccgcatga
     9661 tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat gtcatccctg
     9721 ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt ccccagattg
     9781 gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa aacaattcga
     9841 tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc ataactaaac
     9901 taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt tttctccaat
     9961 taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc gaataaacaa
    10021 gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat atgtaacgtt
    10081 ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct tctctttagt
    10141 taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat ccacaaagca
    10201 acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa aataaatcct
    10261 gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc aaagaaggcc
    10321 ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat catcattact
    10381 ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat cagtggagag
    10441 aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc agtacgtcca
    10501 ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt accaaagata
    10561 gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt tgttttgaac
    10621 ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt gcggcgtcct
    10681 gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc gatgatcgtc
    10741 atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc ttcagaacca
    10801 aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga attcgtttgc
    10861 caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt ggtcactacg
    10921 ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt cagtaccaaa
    10981 ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg gacgattcca
    11041 ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga aatagctgac
    11101 tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc aacgaaaata
    11161 ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat aatcattttt
    11221 cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac cattgccaag
    11281 gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa tgcgttcaat
    11341 cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac cagcgtacca
    11401 ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc tgatgctgac
    11461 aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt tactcccgcc
    11521 attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt ttccataaaa
    11581 agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa taagtaaaat
    11641 agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt ttcaacgttt
    11701 ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg tttagctatt
    11761 tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt aattacctaa
    11821 aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt gaataaaaaa
    11881 ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat tacatctaaa
    11941 acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat tgctacagga
    12001 cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac accgatggtt
    12061 aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga aagagaaacg
    12121 ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct aaatttagat
    12181 tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt ctttgatgaa
    12241 aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa aaatttgacg
    12301 acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc tgtatctgct
    12361 gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg acctactgcc
    12421 atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga gattgctaac
    12481 tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa tgatttggaa
    12541 ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca actcaaaggc
    12601 attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt ttatctggaa
    12661 aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt gacaattttt
    12721 gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg tatgaagtca
    12781 aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac tcgaaactaa
    12841 gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata cgcgtccatt
    12901 atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt cggcaagaca
    12961 acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt actattgact
    13021 gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt tgcggaaagg
    13081 tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc atggacaact
    13141 tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac cgtattttct
    13201 actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc attgccatag
    13261 atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt tgaagcatat
    13321 tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc ggtaaagtta
    13381 cctggagcat accattcttt gccattatcc gcattgaact tcttcatata atcaacagaa
    13441 cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc aagaccaaac
    13501 tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa actaggtagt
    13561 cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat catatacgta
    13621 ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct tcgattaaag
    13681 acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa aacttgattt
    13741 ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac tacagaccct
    13801 ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt ggaaccttgt
    13861 tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat agccactgca
    13921 taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata attccttttc
    13981 aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa attagaacct
    14041 ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc aagggaaact
    14101 tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa ataactttgt
    14161 cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc atctgctgga
    14221 agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact tgctgcatta
    14281 tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc gcgttctgcc
    14341 acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt gtttaattca
    14401 tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc gtttaaatta
    14461 atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt ccctaattgc
    14521 ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc agccagccaa
    14581 aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat cagcgagttc
    14641 agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct cgtatatgtg
    14701 atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta gatcattccg
    14761 cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc ctccatctct
    14821 tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa taacgtaaat
    14881 ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa cgttttgttc
    14941 ttaggttctt gtgcaccttt tcttattttt tctaa
//
LOCUS       DACCDF010000066        14905 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCDF010000066 DACCDF010000000
VERSION     DACCDF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.069"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..506
                     /locus_tag="IVK51_002054"
     CDS             <1..506
                     /locus_tag="IVK51_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6431630.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
     gene            671..1052
                     /locus_tag="IVK51_002055"
                     /pseudo
     CDS             671..1052
                     /locus_tag="IVK51_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1183..2133
                     /locus_tag="IVK51_002056"
     CDS             1183..2133
                     /locus_tag="IVK51_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP6431631.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2651..3106
                     /locus_tag="IVK51_002057"
     CDS             2651..3106
                     /locus_tag="IVK51_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6431632.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            3417..3773
                     /locus_tag="IVK51_002058"
                     /pseudo
     CDS             3417..3773
                     /locus_tag="IVK51_002058"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3786..4196
                     /locus_tag="IVK51_002059"
                     /pseudo
     CDS             3786..4196
                     /locus_tag="IVK51_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4311..4475
                     /locus_tag="IVK51_002060"
     CDS             4311..4475
                     /locus_tag="IVK51_002060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431633.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4626..5605)
                     /locus_tag="IVK51_002061"
                     /pseudo
     CDS             complement(4626..5605)
                     /locus_tag="IVK51_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5610..6377)
                     /locus_tag="IVK51_002062"
     CDS             complement(5610..6377)
                     /locus_tag="IVK51_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP6431634.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6867..7292
                     /gene="lacA"
                     /locus_tag="IVK51_002063"
     CDS             6867..7292
                     /gene="lacA"
                     /locus_tag="IVK51_002063"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP6431635.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            7309..7824
                     /gene="lacB"
                     /locus_tag="IVK51_002064"
     CDS             7309..7824
                     /gene="lacB"
                     /locus_tag="IVK51_002064"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP6431636.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7835..8767
                     /gene="lacC"
                     /locus_tag="IVK51_002065"
     CDS             7835..8767
                     /gene="lacC"
                     /locus_tag="IVK51_002065"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP6431637.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8770..9750
                     /gene="lacD"
                     /locus_tag="IVK51_002066"
     CDS             8770..9750
                     /gene="lacD"
                     /locus_tag="IVK51_002066"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6431638.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9778..10095
                     /locus_tag="IVK51_002067"
     CDS             9778..10095
                     /locus_tag="IVK51_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP6431639.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            10095..11801
                     /locus_tag="IVK51_002068"
     CDS             10095..11801
                     /locus_tag="IVK51_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431640.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11943..13349
                     /gene="lacG"
                     /locus_tag="IVK51_002069"
     CDS             11943..13349
                     /gene="lacG"
                     /locus_tag="IVK51_002069"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP6431641.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13372..13644
                     /locus_tag="IVK51_002070"
     CDS             13372..13644
                     /locus_tag="IVK51_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP6431642.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13665..14564
                     /locus_tag="IVK51_002071"
     CDS             13665..14564
                     /locus_tag="IVK51_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6431643.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4877 a         2468 c         2882 g         4678 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaagata tttggagttc agagagggtg cgtttgattt
      541 tcaaacttcg caacagaacc gatgacacta tcgttcttca tcaacgtaag ttagatttgt
      601 tgaaagagca gaaaaaagga tttctacaaa aaatgtttgt ttgatatttt aaatagaaaa
      661 gagagataaa gtgaagtttt taaatttttt gaactataaa tatttaaact gggtattata
      721 tggtattaca ttatttttta ttatttatta cttttttacc gatgatgata tttttttaat
      781 tttagcaata ctttttcttg tttcatctaa ttttagtgat aacttaattg aagttcttac
      841 agaaccagaa attcggtcta tgacggatga agaacaacag attattaaag ctatgatttc
      901 ggaaaataag aaagacgtag tagttatcaa aaagattaga gagtatacaa atttaggtgt
      961 tttacaagcc aaaaaagtat atgaaaaaaa aaaaaagggt ctataattac aaattagata
     1021 atacattaga agctggagac aagattgtct agataatgtg tgagataaaa tttttggcag
     1081 gttatcatac ataagacaaa atgtttttaa aactgataac gtatttattc cacaacaatt
     1141 acgcatttat ttctgagaaa aatataaagc aggaggatta caatgctgaa aacttattcc
     1201 ttaacaaaag atacattatt agtttcgtca gaatcaaata tcaaaaattc ttctgttgtt
     1261 tatgtttatg gtgacgaacg agaatatatc gaatcttctg aacataaata tgactttaaa
     1321 ataggcaata tactaacgtt tgatgatcga gttgcatatg acacgatgat agataagaac
     1381 gatgaaaaaa gtttgctcgt aagttttttg tttcccgagg tacatgaagg tgggcatcta
     1441 gataccctga cgacatctgt tgttttcttg gtatttaaaa ataagttgat aatttttaca
     1501 aaacaaaaat taaaattcat tcccgaactc ttatccaata tggttttacg tgatgtcaat
     1561 catttcgtgg aggaagtact tttaaagatc atgcaaaaag atttctatat gatgcttaac
     1621 gaccttcgtg gagtcaagga aaaaattgat gatttagact cagaaatatc aacgtccggt
     1681 tcactcaaac caacgtttgg tgatttattg actcttcaaa aatacatgat agctttgtca
     1741 acaacttata aagctaacca taaagcgctt gatttcatta aaaaaaattt cacaactatt
     1801 gacgatattg attttagtac taaaaaaata attgatgatt tatacgatac aagtgataca
     1861 atggatagaa tcattttaag ttacagccaa tatttggaca aattggaaaa tatgataaac
     1921 aatatgattt cgtatcagct aaacatgatt atgaaaaccc taaccgagat atcaattgtt
     1981 ttgactattc cagcaattat ttttggcttt tggggaatca atgtccatgt cccgtttgaa
     2041 aagtcttctt atggtgtttt agccgtattg attatctctg ctatactaag tctaatttgc
     2101 tggcttttga tgcgacgcaa aacatatttg taaagtattt gtgataccct aatatgattt
     2161 aaagcagaat ttctttgttg actttttaaa aggctgattc ctttcaataa tttaatagct
     2221 tcagcggctg ctcagaagtc aataaattgg actattttta ttcagttact taggcaacat
     2281 ttgagatacg attgcgatta taaaaattga tataccaacc aatggctgct tggaagactg
     2341 acatattagc ctcgatatca tcgaataact aaatacagaa aaatacaata aatattatca
     2401 aactagggac tatactagaa aacatcagta cattccttaa ctcagggtct ataattagaa
     2461 aatcgcccct ttgaaaactg attaaaacag cccccattat ctagtgacta gataatgggg
     2521 gctgtttttt tgttaagtga tataataaac cataaaagaa gtttagaaat acggtcctgg
     2581 tcatcaataa ataaacaatt taattaatta tttatttaat tatttaattt cagaaaggta
     2641 tacatgaatc atggtgcaac aaattgtcct acccatcaaa gactccagcg tcttaaagat
     2701 ggtacaagat acacttctcg atagtttccg tgctggtcgc cgaaattaca ccgtttttca
     2761 agttggtaag gccacgctac tacgtgtgag tgatgtcatg acattaaaaa aatcagatgt
     2821 ctataatcca gacggctctg tcaaaaatgc aacctttatt catgataaaa agaccggtaa
     2881 agcaaacacg ttatatttaa agcctgttca gcaagacttg ttgcaatatc atgattggct
     2941 ggcccaagaa aatatcaatt ctgagtggtt atttccctcg acagcccatt atgatcttca
     3001 tatcactgag aagcagtttc ataaggtgat ggcgcatgtt ggtgatctct taggaatcaa
     3061 cttggaagac aaagagaaaa aattgcgagt cagaaaaccg ttttgagata tgaacagcat
     3121 ttgccttcac gccttagcga ttttaaataa tagccttgaa agtgattatg gaaaagccct
     3181 ctacgctcaa tgcaaaattg acgtagaaac tatttttgat cgattgaaag gtgtatttgg
     3241 tatgtgaaga acccatgtca gaggcaagaa cgttgcacct actgacattg gaatgatgct
     3301 tatgagcatg aatctaacca aattatccct tgaggctaga agaaaagcgg aagcttttca
     3361 acacaaagca gccaaaaata aaaaacgcga cgaagcaatc acatttatga ttattcatcg
     3421 cgttttttat atttagagct agttatttgc tagacccact atttacagat gaaacgttta
     3481 tcacctttgg ttttgggcat ttagtggaat tagcagaacc aggtcattat gacgaaaagt
     3541 ggcaaaattg gaaacttgaa tctttgccga tttttcctga tcgatacgat tttgaagttg
     3601 caaaagataa gggaaagcag tttaaaattg ttgcagaact tctcaaaaag gcaaatacaa
     3661 ttattgttgc aacagatagc gacagagaag gtgaaaatat cgcctggtcg attatccata
     3721 atgcaaatgc cttttcaaaa gataaaacat ttaaaagact atggatcaat agcttagaaa
     3781 aagatgtgat ccgaagcggt tttcaaaatt tgcaaccagg aatgaattac tatccctttt
     3841 atcaagaagc gcaaacacgc caaattgccg attggttgat cggcatgaac gcaagccctt
     3901 tgtatacgtt aaatttacaa cagaagggcg tacaaggtac attttcacta ggacgtgttc
     3961 aaacgcccac cttatacctt atttttcagc gccaggaagc catagagaat tttaaaaaag
     4021 aacctttttt cgaggtggaa gctaagtaaa acaagggtca tttaagggcg ttctaagccc
     4081 cacacagcgt tttaaaaccc aagaggagct ttttagcttt tgtttcttct aaacaagcta
     4141 aaataggcaa tcaagagggg ataattgctg atgttcaaac caaagagaag aaaacgaata
     4201 gtccgagttc gttttcttta agtagtttgc aatcaaaagt caatcagctt tataaagcga
     4261 cagcgagcca aactttaaaa gctatgcaag gactgtatga agcaaaatta ttgagttatc
     4321 caagaacaga tacaccattt attacagaga acgaatttgc ttatttaaaa gcgaattttg
     4381 gcaaatatag cggtttttta ggacttgatc ttgaaatgtt gttatcattg ataatgatac
     4441 gttcaaacaa cagcacccaa attttgacga attagtgaaa ctctatgaaa aagacgtagt
     4501 aaaacatgca acatcctatt ctaatcagct ggtaaagctt aattaggtta tataagcttg
     4561 aaaataaagg gtttgtccaa tatggacaag cctttttctt atgttttgcc acagatttgc
     4621 cacacttatt gtgtaagagt atcaaggata ggtataattt gatactttgt ttttttagtg
     4681 atgtgcaaat aaatttctgt tacacgacta cctctagaat gccctacacg gtcttgaatt
     4741 gcttctaggg gtattcctta ctctgctaag aaactaatat gcgtatgacg gaatatatgg
     4801 gtcgtaatat gtttattaaa actaagttta ttatcaatat caattcctgc tcgttttgga
     4861 ttaccttttc gatttaattt tccattttca ggtgagttga ggaaattgtt aaggacacta
     4921 taatgaagag gtgttccatt atagtcgggt ttatgacgac ttttggaagt aaaaatccat
     4981 ccattatctt tataatcagg attgaatctc atatgttggt gatttaactc aatttcctct
     5041 ttaagaattt ctatagccct tttagataat tggacatttc ggtaactttg taagtttttt
     5101 acattacctt catagctatt ttctgcagct cctccgtgag tggactgact aatgatgata
     5161 ttttgctttt caaaatattg ataggcacca atattttgga gtttcgactc tgtaagtcaa
     5221 cagggccatt agttagaata cctattggga tgcggttttt atgtagagcc agcagtagtt
     5281 gatgtatctc aggaatcatc gtaatctgat cgagttcata ttcatagtca gcttggaagt
     5341 ctagagcttc tgtcctagaa atactatcga tgccaaattc ttccatagtc ttctgacagc
     5401 gaaaaattct aagctgctca atactgatga gttttttcgt gtatttagga aaagcaacat
     5461 cagaccaata tctgaaacgt ttgtaaatgt tatctatttg ttggatatca atcgttggaa
     5521 aacatttctc aacgctacgt ttaaagggga gttgatggtc ataaagagtg tcatcaaggt
     5581 caaaaacaat atttttataa gtcataattc tagtccgctt ttaaaatagt agtgtactgg
     5641 ctaaattcct ctaagtgttg agggctaatc tggttatctg taatgatggt atcgagttga
     5701 tctagattat agaagttaaa gaaatcgtat cgatcgaatt tagtactgtc tactaataag
     5761 aatttttcta cagcattgtt tagggcaagt tgttgaatca caccttcctt gtcactatat
     5821 gtagcaatag aattatgggt aacagcattt gcactaacaa aagctttggc aaatctaatt
     5881 gcttttaaat ttgtcgaagc cattgaacct acaaaagctc cagttatttc tctatattca
     5941 ccgccaagaa gcaataaatc aatggtttcg ctatcattta gaatcaagaa cacagggaga
     6001 ctatttgtaa tgacacgaat tttataacct ttacgacctt tcaattctaa tgccagttgt
     6061 tcaagtgtag ttccaggtcc aataaaaata gtatcgccat ctttgattaa agagcaggct
     6121 tttttagcaa tgtcaatttt ttcttttgta tttagacttt tcttctcgat atgtgtcttt
     6181 tcaagtggct ttttagagga aagcaattgt gcaccaccat gagtacgtgt taaaagtcca
     6241 tcagcttcta gagcatcaag gtctctacgg gctgtcatat cggaaatatc tagttcatct
     6301 attatttctt ttatggttat ggtcccatca atctttaaca tatctaaaat tttttctaat
     6361 cgtcgttttt tgttcatatg aagacccttc tttcataaag taattttttt ccaaagataa
     6421 ttctctttta attgtatcat aaaagacaat attttcaagg taaaacaaac aatttcaaac
     6481 aaaaacaaat gttagatgat gaaataagaa cagaggattg acgtatatta gcttaggtca
     6541 gattttgtat aagacgaaaa taaagtagga cctcttaatc agtaagttat agaaagtaaa
     6601 agacttttgt aatacttgaa tagatatttc acgtccattt tgtgatggat taaatgaaca
     6661 aaaatgaaca ataatttaac ggtgttatct attttttaaa aaaacaaata aaaaaaacaa
     6721 aaaataaaca aaaaaagtgt tgctttttgt ttgaatgctt gatattatat aaacaaagaa
     6781 atgatgaaaa cgttttttcg aacattttgc aaaatgtttt ctacttctac gtagcatttc
     6841 tttctaaaat ttaggaggtg gtcaaaatgg ctattgttgt tggtgcagat ctcaaaggta
     6901 cgagattaaa agatgttgtg aaaaattttc ttgtagaaga aggttttgag gtaattgatg
     6961 tgactaagga cggacaagat tttgttgatg ttaccttggc agttgcctct gaagtaaata
     7021 aagatgagca aaacttaggt attgtaattg atgcttatgg agctggtcca ttcatggtag
     7081 caactaaaat taaaggtatg gttgcagcag aagtttcaga tgaacgctca gcttatatga
     7141 cacgtggaca taacaattca cgaatgatta cggttggcgc tgaaatcgtc ggtgatgagc
     7201 ttgctaaaaa catcgctaaa ggtttcgtca atggtaaata tgatggcgga cgtcatcaaa
     7261 tccgagtaga tatgcttaac aaaatgtgtt aagaaaagga gttaaggaat gagaatcgca
     7321 attggatgtg accacattgt cacagatgaa aaaatggcag tatcagaatt cttgaaatca
     7381 aaaggacatg aggtccttga tttcgggaca tatgaccacg cacgtactca ctaccctatc
     7441 tatggtaaaa aagtcggtga agcagtagta agtggtcaag cagacttggg agtatgtatc
     7501 tgtggtacag gtgtcggcat taacaatgca gtcaataaag ttccaggtgt tcgctcagcg
     7561 ttggttcgtg atatgacatc agctatatat gcaaaagaag agttaaatgc gaatgttatc
     7621 ggtttcggtg gaaagattac tggtggtctt cttatgaatg acatcattga agctttcatt
     7681 gaagctgagt acaaaccaac agaagaaaat aaaaaattga ttgcaaaaat cgaacacgtt
     7741 gaaacacata atgcacatca agcagatgaa gatttcttta cagaattcct tgaaagatgg
     7801 gaccgtggtg actaccacga ttaaggtata acaaatgatt ctgacagtca cactaaatcc
     7861 ttcagtagac atctcttatc ctctagaaac attgaaaatt gatacagtga atagggtgaa
     7921 agatgttagt aagacagctg gtggaaaagg cttaaatgtt actagagtct tgtatgaatc
     7981 tggtgataaa gttactgcta caggtttctt aggtggtaaa attggtgaat ttattgaaag
     8041 cgaattagaa caatctcctg ttagtccagc tttttataaa atctcaggaa acactagaaa
     8101 ctgtattgct atcttacacg agggtaacca aacagaaatt ttggaacaag gaccaacaat
     8161 ttctcatgaa gaagcagaag gctttcttga ccactatagc aatcttatta aacaatcaga
     8221 agttgtgaca atttcaggaa gtttaccttc aggacttccc aacgattatt atgaaaaact
     8281 tatccaactt gcttcggatg aaggagtagc agtcgttttg gattgttcag gtgcacctct
     8341 tgaaacagtt ctaaaatcgt cggctaaacc tactgccatc aaacctaata atgaggaact
     8401 ttcacagttg cttggaaaag aagtaacaaa agatattgaa gaactcaaag acgttcttaa
     8461 agagtcactt tttagtggta tcgaatggat agtggtctca ttaggtcgaa acggtgcctt
     8521 tgcaaaacat ggtgatgttt tctataaggt agatattcct gatatcccag ttgttaatcc
     8581 agtcggatca ggggactcaa cggtagctgg tattgcatca gctttaaata gtaaaaaaag
     8641 tgacgctgac ttattaaaac atgcgatgac attgggtatg ttaaatgctc aagaaacaat
     8701 gacagggcat gtgaatatga ctaattacga aacactaaac tcacaaattg gagtaaaaga
     8761 ggtataaaaa tggtacttac agaacagaaa cgcaaatctt tggaaaaaat ttcagataaa
     8821 aacggtgtta tctcagcttt ggcatttgac caacgtggtg ctttgaaacg tttgatggca
     8881 cagtatcaag atacagaacc aactgtagct caaatggaag aactaaaggt tttggttgct
     8941 gatgaactta caaaatacgc ttcttcaatg ttgcttgacc ctgaatatgg acttccagca
     9001 acaaaagcat tggataaaga agcaggtctt cttcttgcct atgaaaaaac aggttatgat
     9061 acatcaagta caaaacgttt gcctgactgc ttggatgttt ggtctgcaaa acgtatcaaa
     9121 gagcaaggcg ctgatgccgt taagttcttg ctttactatg atgtagacag ctcagatgaa
     9181 cttaaccaac aaaaacaagc ttatattgaa cgtgttggtt ctgaatgtgt cgctgaagat
     9241 attcctttct tcttggaaat ccttgcttat gatgaagaaa tctcagatgc aggttctgtt
     9301 gaatatgcaa aagtgaaacc tcgcaaagtc attgaagcga tgaaagtttt ctctgatcca
     9361 cgctttaaca ttgatgttct taaagtagaa gtaccagtta acgttaaata tgtcgaaggt
     9421 tttgctgatg gtgaagtagt ttacagtaaa gctgaagctg ctgacttctt taaagcacaa
     9481 gaagaagcga ctaaccttcc atacatctac ctaagtgcag gtgtatctgc taaattgttc
     9541 caagaaacat tgcaatttgc tcacgactca ggtgccaagt ttaatggtgt gctttgtgga
     9601 cgtgctactt gggcaggatc tgtagaacct tacatcaaag aaggtgaaaa agcagcacgt
     9661 gaatggttgc gtactactgg attcgaaaat attgatgaac ttaacaaagt tcttgttaaa
     9721 acagctagtc catggactga taaagtatag taaaaacata aaacggagga tattgttatg
     9781 aacagagaag agatgactct cttagggttt gaaattgttg cttatgctgg agatgctcgc
     9841 tctaaacttt tagaagcgct taaagcggct gaaaatggtg atttcgctaa ggcagatagt
     9901 cttgtagtag aagcaggaag ctgtattgca gaggctcaca attctcagac agctatgttg
     9961 gctcgagaag cttctgggga ggaacttcca tacagcgtta ccatgatgca tggtcaggac
    10021 cacttaatga ctactgtctt attaaaagat gtgattcatc acctcatcga actttataaa
    10081 agaggagcaa aataatgcat aaactcattg aacttattga gaaagggaaa ccgttctttg
    10141 agaaaatctc tcgaaacatc tatcttcgtg ctattcgtga tggatttatc gctggtatgc
    10201 cagtcatcct tttctcatct atctttatcc ttattgccta tgtcccaaat gcttggggat
    10261 tccactggtc aaaagacatt gagaccttct tgatgactcc ttatagctat tcgatgggta
    10321 tcctagcatt ctttgttggt ggtactaccg ctaaagcttt gacggactca atgaaccgtg
    10381 acctacctgc gaccaatcag attaacttct tatctacgat gctagcgtct atggtagggt
    10441 tccttttgat ggcagctgag cctgcaaaag aaggaggctt cttgacagcc ttcacgggaa
    10501 caaaaggtct tttgacagca tttattgcag cctttgtgac agttaatgtt tacaaggtct
    10561 gtgtgaaaaa taatgttact attcgtatgc ctgaagaggt tccaccaaat atctctcaag
    10621 tatttaaaga cttgattcca ttcactgtag cggttgttct cctttatgga tttgaactca
    10681 ttgttaaggg aactcttggt gtaaccgttg cagaatcaat cggtaccctt cttgctcctc
    10741 tcttctcagc tgcagatggt taccttggaa ttacacttat ctttggtgct tatgccttct
    10801 tctggtttgt tggtatccac ggtccttcaa ttgtcgaacc agcaatcgct gctatcactt
    10861 atgccaatat cgatgccaac ttgcatctta tccaagctgg acaacatgca gacaaagtta
    10921 tcacttctgg tactcaaatg tttattgtta ccatgggtgg aacaggagct acattgattg
    10981 ttccattctt gttcatgtgg atttgtaaat cagaacgtaa ccgtgccatc ggacgtgcct
    11041 cagttgttcc aacattcttt ggggttaatg agccaatctt atttggtgca ccaatcgtat
    11101 tgaatccgat tttctttgta ccgttcattt tcgctccgat tgtcaacgtt tggatcttta
    11161 aattctttgt tgacacattg aacatgaact cattctctgc caaccttcca tgggttactc
    11221 ctggtccgtt gggaattgta ctcggtacta acttccaagt tctatcattt atcttagccg
    11281 gactcttggt agttgttgat actatcattt attacccatt tgttaaggta tacgatgaac
    11341 aaattcttga agaagaacgt tctggtaaaa caaacgatgc tcttaaagaa aaagtagctg
    11401 caaacttcaa tactgctaaa gcagatgccg ttcttggaaa agcaggcgtt gctaaagaag
    11461 atgtagcagc aaacaataat atcacaaaag aaactaatgt ccttgttctt tgtgcaggtg
    11521 gaggtacaag tggcttgctt gccaatgcct tgaacaaagc agcagcagaa tacaatgttc
    11581 cagtcaaagc ggcagcgggt agttacggtg ctcaccgtga aatgttgcca gagtttgact
    11641 tggttatctt ggctccacag gtcgcttcaa acttcgatga tatgaaggct gaaacggata
    11701 aattgggcat taaacttgca aaaactgaag gagctcaata tatcaaattg acacgtgatg
    11761 gacaaggtgc tcttgcattt gttcaacaac agtttgatta ataaatttgt aaaagttatt
    11821 tgagacgaga ttattctttc aattccttat aatattgtta accatatcat tgcatttcat
    11881 ttccttggtt tccggaactc agaatatcgt ctcaaatgct ttttttgaaa ggatttatag
    11941 ttatgactaa aacacttcct aaagatttta tttttggtgg tgctacagct gcttatcaag
    12001 cagaaggtgc gactcacacg gatggtaaag gcccagttgc atgggataag tatcttgaag
    12061 acaattattg gtacacagca gagcctgcaa gtgattttta ccataaatat cctgttgatt
    12121 tagaattagc tgaaaaatat ggcgttaatg gtattcgtat ttcaattgcc tggtctcgta
    12181 ttttcccaac tggctatggc gaagtaaatg aaaaaggtgt tgaattctac cataatcttt
    12241 ttgccgagtg ccacaaacgt catgttgagc cattcgtgac tttgcaccac tttgacacac
    12301 cagaagttct tcactcagat ggagatttct taaaccgcga aaatatagaa cactttgtag
    12361 attatgccgc tttctgtttt gaagagttcc cagaagtaaa ctactggaca actttcaatg
    12421 aaattggccc aattggagat ggtcaatact tggttggtaa attccctcca ggcattcaat
    12481 atgatcttgc caaagtcttc caatcacacc acaatatgat ggtttctcat gcacgtgctg
    12541 tgaaattgta taaagataaa ggttataaag gcgaaattgg tgtagtacat gcgttgccaa
    12601 ccaaataccc ttatgatcca gaaaatccag cagatgttcg tgccgctgag ttagaagata
    12661 tcatccataa caaatttatc ttggatgcaa cctaccttgg acactattca gataaaacga
    12721 tggaaggggt caaccacatc ctagctgaaa atggtggaga acttgatctt cgtgatgaag
    12781 acttccaagc acttgaagca gctaaagatt tgaacgattt ccttggtatc aactactaca
    12841 tgagtgattg gatgcaagcc tttgatggtg agactgaaat cattcacaat ggtaagggtg
    12901 aaaaaggaag ttctaagtac cagattaaag gtgttggtcg ccgaatcgca ccagattatg
    12961 tgcctagaac ggattgggac tggattattt atcctgaagg cttgtatgac caaatcatgc
    13021 gagtgaaaaa tgattatcca aactacaaga agatttacat cactgaaaac ggtcttggat
    13081 acaaagatga gtttgtagat aataccgttt atgatgatgg tcgtattgat tacgtgaaga
    13141 aacacttgga agttttatca gacgcaattg cggatggtgc aaatgttaaa ggttacttca
    13201 tctggtcact tatggatgtc ttctcatggt caaacggata cgaaaaacgt tatggactat
    13261 tctatgtaga ctttgacacg caagaacgct atcctaagaa atcagctcat tggtataaga
    13321 aagtagcaga aactcaagtg atagagtaat attacttgaa aatgtaaaaa attgttatcc
    13381 gatactttag aaaaagtaca acacttattt agaccaggct ttgaaaaggt atatattgtt
    13441 agtttcgaag attgtcctat gattcctgag cttgaagcta ccccgttgct aaaatgtggt
    13501 aaatggtatg tttcaactgg aaaagagtgg atttgccatt ctgatttaga gttatcagca
    13561 tttgaatggg aatttttaca atcactcgat gtagaaatac gtgagactat tcacttcgag
    13621 gtaaattatt tgcctttcca ataataaaaa gaaaggaatt agttgtgact atcgaattaa
    13681 aaaacgagta tcttactgtt cagtttaaaa cacttggtgg tcaactgact tcgattaaag
    13741 acaaggatgg gatagagtat ctctggcaag ctgatccaaa ttattggaat ggacaggcgc
    13801 ctattctatt tcctatctgt ggtagtttac gaaatgattg ggctatttat agacctcaag
    13861 aaagaccctt ttttacaggt cttattcgta gacatggttt tgttcgaaaa gaagaattta
    13921 ttctggaaga agtcaatgat aatagcgtga cctttagtat taaaccaaat gctgagatgc
    13981 ttgataatta cctatatcag tttgaactga gagttgttta taccttgaat ggtaaatcaa
    14041 ttagaaccga atttcaggta acgaacttgg aaatggaaaa aacaatgcct tactttattg
    14101 gagcccatcc agcattcaat agtcctttag tagaaggtga aaactatgag gattattata
    14161 ttgaatttag tgaggttgag tcttgctcga taccaaagag ttttcctgag acaggattat
    14221 tagatttgca agatcggaca ccttttttgg aaaatcaaaa aattctcgat ttggactact
    14281 cacttttttc ccatgatgcc attacacttg accagctcaa gtctcgtagc gtaacgcttc
    14341 ggtcaaggaa gtctggtaaa ggtcttcgag tagattttga tgattttccg aacttgattc
    14401 tgtggtctac aactaataaa agtccattca ttgctttgga accttggagt ggtctctcca
    14461 cttcacttga agaaggtaat ttcttagaag ataaaagaca ggtaacgaaa atttcacctc
    14521 aagaaacttc tagaaaaatc tatgatatta ctatccttaa ttaaaatttg ttattatttg
    14581 agtcttcaat aagatttgaa tactcttacc ttgtgatgat agattggtct tctccgtgcc
    14641 agtcttacat cgcaaggttt ttaagttttt tgtgggtcta tgtcaactgt agtgggtgac
    14701 gaaaagctaa tatctaaaga cagtctggta gtaaaaaata agattattta ctacttcttg
    14761 atttaaaata atagtggagc cattaatttg tggcaaattt aatttaatat tttgcctcaa
    14821 attagccaca ctttttatag aactaagagg aaaaatgaaa ttttcttcct attaatatag
    14881 ttgtttgaaa tcttaaatat agcgt
//
LOCUS       DACCDF010000067        14293 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCDF010000067 DACCDF010000000
VERSION     DACCDF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.070"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            133..2604
                     /locus_tag="IVK51_002072"
     CDS             133..2604
                     /locus_tag="IVK51_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6431644.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2707..4443)
                     /locus_tag="IVK51_002073"
     CDS             complement(2707..4443)
                     /locus_tag="IVK51_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP6431645.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4440..5144)
                     /locus_tag="IVK51_002074"
     CDS             complement(4440..5144)
                     /locus_tag="IVK51_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431646.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5275..5778
                     /locus_tag="IVK51_002075"
     CDS             5275..5778
                     /locus_tag="IVK51_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP6431647.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5738..6076
                     /locus_tag="IVK51_002076"
     CDS             5738..6076
                     /locus_tag="IVK51_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP6431648.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6097..7515
                     /gene="ffh"
                     /locus_tag="IVK51_002077"
     CDS             6097..7515
                     /gene="ffh"
                     /locus_tag="IVK51_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP6431649.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7627..8205
                     /locus_tag="IVK51_002078"
     CDS             7627..8205
                     /locus_tag="IVK51_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP6431650.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8209..8730
                     /locus_tag="IVK51_002079"
     CDS             8209..8730
                     /locus_tag="IVK51_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6431651.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8809..9363)
                     /locus_tag="IVK51_002080"
     CDS             complement(8809..9363)
                     /locus_tag="IVK51_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6431652.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9616..9891
                     /gene="rpsP"
                     /locus_tag="IVK51_002081"
     CDS             9616..9891
                     /gene="rpsP"
                     /locus_tag="IVK51_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP6431653.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9903..10154
                     /locus_tag="IVK51_002082"
     CDS             9903..10154
                     /locus_tag="IVK51_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP6431654.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10279..11070
                     /locus_tag="IVK51_002083"
     CDS             10279..11070
                     /locus_tag="IVK51_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431655.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11240..11761
                     /gene="rimM"
                     /locus_tag="IVK51_002084"
     CDS             11240..11761
                     /gene="rimM"
                     /locus_tag="IVK51_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP6431656.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11751..12512
                     /gene="trmD"
                     /locus_tag="IVK51_002085"
     CDS             11751..12512
                     /gene="trmD"
                     /locus_tag="IVK51_002085"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP6431657.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12648..12995
                     /gene="rplS"
                     /locus_tag="IVK51_002086"
     CDS             12648..12995
                     /gene="rplS"
                     /locus_tag="IVK51_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP6431658.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13253..13546
                     /locus_tag="IVK51_002087"
     CDS             13253..13546
                     /locus_tag="IVK51_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP6431659.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         4794 a         2567 c         2905 g         4027 t
ORIGIN      
        1 tttttttttg ctatatgaca ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt
       61 tccatatctg ttctatactg gataggagaa gtttggaaat atccagaaaa cgaaatcaat
      121 cgaggtagcc acatgaagaa taaatggata aatcgaagta aaaaaaagaa agtaacacaa
      181 agatcatcag ataaagtcat ccgtcttttt ccatcgtata aagaaggatt aacggcagat
      241 caagtcaagg aacgaataga aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa
      301 acaaatcagc aaatcgtatt ggaaaacata tttacgtatt tcaatttgat ctttcttatc
      361 ttagccattt tgctttgttt agtagaatcc tataagaact tgactttttt accagttatc
      421 atagccaata caggaatcgc catttttcag gaaattcgat ccaaaaagat cttggacgaa
      481 ctaaatgttt taaatacagc aaaagtcaat gtcatacgag acgggataga acaaacaata
      541 gatatagaag accttgtaat agatgacatt gttattttag agactggata tcaaattcca
      601 gcagatgcag aggtcgtgga agggaaactg caagttaatg aggctttatt gacaggcgaa
      661 gcagatgaaa taaccaaaga aataggtgat ccattaatgt ccggcagttt cattgtttcc
      721 ggaaaagctt acgcccgatt agacaaagtt ggagcagatt cgtatatttc ccagctgaca
      781 gtcaaagcca agacaatggg agaaggagaa cagtcggaga tgattgcttc tcttaatcaa
      841 ttgatcaaat ggattgggat catcattatt cctatcggta tcctgctttt ttctcaaagt
      901 tactttttta atggaaacac gatcaaagaa agtgtcgttg caatggaagc agcattgatc
      961 ggtatgatac ctgaaggact gtacttgctg acgacgattg cattagcatt aagtgcgact
     1021 aaactagcaa agcaaagagt tcttttacac aatatgaaaa gcattgagac attggcaagg
     1081 gtaaatgtcc tttgtgtaga taaaacagga acgatcacag aaaataaaat gtctgttcaa
     1141 aaaatgatcg tctctaaaaa acaagaaata atcagtcagt cagatcaatt ggaagcatgc
     1201 attgctgact atgcaaaagc gatggcagga gacaatgcaa caatggaagc tgttaaagca
     1261 tattttacaa aaaccactga tcaatcctat caccatgtta ttcctttttc ttctgtccaa
     1321 aaattcagca gtgtagcgtt ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt
     1381 ttgcgcgatc aattgccgga atatgcggaa gagtttgttt ctttcgctga aaaaggctat
     1441 cgcgtgttgg tctttggtgt gtacgatggg attcttgaag aaccagtatt gacagaagca
     1501 gtcacaccat taggctatat tctgattgct aatcctatca gaaaagaagc aagagccaca
     1561 tttgattact tcaaaaaaca gcaagtagcc atcaaagtga tttctggaga taatccgctc
     1621 accgtatcca atgtagctgt ccaagcaggg atttcacatg ctgatcagta cgttgatgca
     1681 tctaaattat cagaagaaga atatgaagca gctgtcgaaa aatatacagt ttttggacgc
     1741 gtcaagccag aacaaaagaa aatcttcgtt caattgataa agcgtaacaa tacagtagct
     1801 atgacaggtg atggcgtgaa tgatattcta gccatgaaag aagcagactg cagtatagcg
     1861 atggcttccg gaaatgaagc cgccatgcag gcctcacaag tggtcttact tgattctgac
     1921 ttttcacgca tgccagaagt tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga
     1981 tcagccagct tgtttttagt gaagaatatt ttctcctttt tgctctcact gttttcagtg
     2041 atctttgctc tgacctatcc tttagaacca tcacaaatca cattgattag tttgtttact
     2101 atcggacttc catctttctt gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa
     2161 ttcatcatga atgtgatgga aaaagctgtg ccaggtggac taacagatat gatcgtagtc
     2221 ggtgcgttgg tgatctgcgg ggtgactttg aatctaaata aaacagatgt ttctacagca
     2281 tctaccatgc ttttgattgc agtagggttc ctcgttctct ataaaatctg ttcaccgctg
     2341 aataaattca gaagtcagat cattcttttc tgtgctagcg gtaacttttt ttcggtcatc
     2401 tttttgcata agctgttttc aatcaccggt atctcagctg tatcactttt gctgttgagc
     2461 attctatttt tctcggcaga ttcgattttt aggcatttga ccacattggt agaatatttg
     2521 tttgaacgta aaaatgaaaa agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta
     2581 tcgatattta aacgagaaaa ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac
     2641 cactcgtgta tctttcgaag caggatgagt catccaactt taaaacgcac gagcagttgc
     2701 tcttttttac attttcggaa tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc
     2761 tacagtaatc gtaccattaa ggagttgcac gtagtctttt acaatcgcta accctaatcc
     2821 ggtcccgcct gaatgtcggc ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg
     2881 ttcctgatcc tcaatgtcga tacctatacc aaagtcttgg acagaaaacg atagatcatc
     2941 tgttgcctga tagcggataa taatctcgct gtcgcttttt gaatattgga cagcattttc
     3001 aatcagattt ttgataattg gataaaggat atctgtttta gtagtaaatg gttcgttctt
     3061 tccaatcaat cgaatcgtca accgtttctt ttcgatgatt ggctgataat tttgaacgat
     3121 ttgctggaaa taaggttctg tataaattgt ctggatctcg taagaattct cgccatcttt
     3181 tgacagctgg atgatttccc gaatcagttt atctaaccgg atcgcatctt tttgcatgat
     3241 ctcaagaaaa gaagtcagag tttgcgggtc atcttttgca ccatccagta aagtctcggt
     3301 aaaaccaatc agtgaagtca caggagtttt taattcgtga gagacgttgc caacgaaatc
     3361 cttttgtagc ttttctaaac gtcgcacttt cgttaagtca tacgccaccc ctagaatctg
     3421 tccggtacct tctgaattag taaagtagcg caaactcaag tctaatgtct gccgaccttc
     3481 tgtaagggtg atctcctgat ggatcagcgg atgctcagga gtcacttgat gaatcagctg
     3541 aatgagtttt ggctcttgaa tgacttctgt gtaccgctgt tcaggttgat agcttaccac
     3601 gtttaaatgt agctgcatct ttggattaag cagtagtaaa cgagaatcag catcatcaat
     3661 caggaatacg ccaatcatca gttcgttcag taacgtatat aattgctctt ctgtggtagt
     3721 acacgcccga tacatcttac tcatttgttc gctcaacgca ttaatcgtct gatacaattc
     3781 ttcccattga tcgggtgttt gcatgattac ttccgcctga ttaggatttt gcaccatttt
     3841 ttttagtacg ggcagcacgg ttttcaatgg ctggtttttt tgatagatca gataatagat
     3901 cattcctgca agaacaagga agaaagacag aaagaagaaa aagacccaac ggcgaaaact
     3961 ctcagttctc ggtaaaaaaa cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg
     4021 gccacttttt ttgactggta atgcgacata taaaagatcc tcattcaaag tagtactttt
     4081 gcgcaaagcg ctacctaggt tcccaccatt caatacggct ttgatttcgg gacgggagtt
     4141 tcgctgttca ttcaatgttt cattgtttgt gtcaaataag atctttccag tagcatccat
     4201 taaagtaatc cgttcgttcg actgatggac gaattcttcc aaagcagttt tgttttgcgc
     4261 tgcttggagg ttttcgacat ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta
     4321 gctttcttgc tgttccacga tctgctgttg aaaataatgg gaaatcaatt gccagccacc
     4381 aaaaaacaaa ctaactaaaa gaataagaaa gaagagacgt tcataaattt ttttccattt
     4441 catcgtttgg gttcctgaaa cttatagcca aatcctctaa tagtcaccaa gtactttggg
     4501 tgttttggat ctcgctcgat tttttctcgc aaatgactaa cgtgtacatc tacgattcgg
     4561 ctttgtcctg cgaagtcaaa attccaaatg cggtctagca atgtgtctcg gtcgatcaca
     4621 cggtcttttc gtttcatgaa ataaacaagt aattcaaatt ctttcggtgt tagttcaatc
     4681 agatggtcgt ctacggtcac ttgataattg gtcaaatcag cagtgatttg tccaatcgat
     4741 agatattcag gctcagcttc atcttttgat tgcttacgag gttcaattcg tctgaatatt
     4801 gctttcatac gggctaatac ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca
     4861 cctatttcta ggccaatgat ccgatccact tggtcatctt tagccgtcag catcaaaatc
     4921 ggtgtttcat ttttttcttg acgcagccgc tgagtgatct ccatgccatc catcgttgga
     4981 agcatcacat ctaaaatgat gaaatcaaat gattgttcta atgctaattc taatccttcg
     5041 cctccatctg tggcactggt taccttgtag ccttcttttt ccaaattgaa cgtcaagagc
     5101 gttacaatag aaggttcatc atcaacaact agtatttttt tcattcaaat ctcctttaaa
     5161 taggtttcat tctgtttata ttttagcata gaatcagtac aaagggctat tttacaaaag
     5221 aaataaatgg tataatagga aggcactttg ctgaatagaa aggaagagca gtcaatgatt
     5281 ggaatcagtg catgtttagg cggtctggcc tgtcgctatg atggacagaa aaaagaagta
     5341 ccagaattga tggaactagt tgaaactgga gaagctgtca tggtctgtcc agaagtgatc
     5401 ggcggattac caattcctag agatcccgct gaaatcattg gtggaaatgg attcgatgtg
     5461 tggaaagatc aagcaaaagt atggacgaca agcggcgaag atgtaacgga agcgtacaaa
     5521 aatggcgcga tcaaagccta tcaaaaattg caggaaaaac acattgatct agtcatcatg
     5581 aaggaaaaaa gcccttcatg cggaacccat tcgatttatg acggaagttt ttccggaata
     5641 aaaaaaaatg gtgccggagt ggctactgct tattttattc agcaaaaaat gactgtgctc
     5701 tctgatgaag aatggctgaa aactagaggt gagcagaatg gaaatcgaaa agaccaatcg
     5761 aatgaacgcg ctctttgaat tttattcgac cttattgaca gaaaaacaaa tgaactacat
     5821 ggaattatat tatgcagatg atttttcatt aggtgagatc gcggaagaat acgaagtaag
     5881 ccgacaagca gtatatgaca atatcaagcg gacaagcaag atcttggagg attatgaaaa
     5941 aaaactccat cttttttcta attatattgt tcgagaacag gtattggaaa atatgactgc
     6001 atatattaca gataagtacc cggaagataa aaaactactt gaatatgtac agcaaattca
     6061 agcaatcgaa gaataaataa aaggaatgaa gaaaatatgg catttgaaag cttaactgaa
     6121 cgtctccagc aggcgatggg aaaaataaga aaaaaaggaa aagtatcaga agctgacgta
     6181 aaagaaatga tgcgggaaat ccgccttgcc ttactagaag ccgatgttaa cttgcaagta
     6241 gtcaaagatt ttactaagcg cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt
     6301 ttatcacctg cacaacaaat cgtaaaaatc gttgatgaag aattgacgat cacacttggt
     6361 tctgaaacag cagagttaaa taaatcacca aaaataccaa ctgttatcat gatggctggt
     6421 cttcaagggg caggtaaaac gacctttact ggtaaattag ccaattattt aaagaaaaat
     6481 gaaaatgctc gtccgctcct aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa
     6541 ttgaaagtat taggtcaaca gctagacgtt cctgtatttg atatgggaac tgaagtcagc
     6601 ccggtagaga tcgtccgtca agggatggag ttagcaaaag aaaagaaaaa cgactatgta
     6661 ttgatcgata cggcaggacg tctgcatatc gatgaaacat tgatggatga gttgaaacaa
     6721 atcaaagaat tgacgcaacc aaatgagatt ttgcttgttg tcgatgccat gactggtcaa
     6781 gatgccgtca acgtagctga tagcttcaac cagcaattag gtattacagg ggtcgtcatc
     6841 actaaacttg atggggatac tcgaggcggg gcagcactat cgatccgctc agtgacaggc
     6901 gcaccaatca aattcatcgg ttctggtgaa aaattgactg acttagaagt attccaccca
     6961 gatcgtatgg ctagccgtat ccttggtatg ggggatatgc tgacattgat tgaaaaagcg
     7021 cagcaagact acgacgagaa aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca
     7081 ttcgatttca atgactttat tgaacagctt gatcaagtga tgggtatggg cccactggaa
     7141 gatctaatca aaatgatccc aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta
     7201 gatccaaaag atgttgaacg caaaaaagcg atggtttatt ctatgacgcc agcagaaaga
     7261 gcaaatcctg atctgttgaa cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac
     7321 agtgttgttg aagtgaatcg aatgatcaaa caattcaagg aatcaagaaa aatgatgcag
     7381 caaatgtcca aaggggatat gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc
     7441 aaaggaaaac taggcaaaat ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag
     7501 aaaaaacgga aataagatga aaaaaacagc ttcggtacag actgaaaatg tctactgccg
     7561 gagcttttct atcagcaatt tttattcttt actaatacaa ttactctaaa cgaagaggtg
     7621 gcacagatga gatgtccgtg gagtgaacaa acagaaagta tgcggcaata tcatgatact
     7681 gtctggggtg ttcctgaata tgatgaccaa aagctgttcc gaaaattgat gcttgatatt
     7741 aatcaagccg gattgagctg gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca
     7801 tatgatcgat tcgatatcaa aaaagtcgca aattatggag aaacaaaaat cagtgaacta
     7861 atggaaaaca aaggaattat tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc
     7921 aaagcagtct tgaaaattca agaagaaatc ggcagttttt cagagtatct ttggtcgttt
     7981 tctaatggaa aaatcgttga ttcttcttat cattcacaag aagaagtccc aacaacaagt
     8041 cctctttcag atgaattagc aaaagatcta aagaaaagag gatttaaatt tatcggaagc
     8101 acaacaatct atgctttttt agaagctgtc gggatcatca atgatcacct tgacagttgt
     8161 ttccgcaaag aagaggtcgt acgtatcggc agaaagggcg aataaagcat gcgaaaaatc
     8221 atattttatg gtgctataag tttagatggc tatttggcaa cgaaaacgga tgatcttcaa
     8281 tggctttttg acactccaac aggtaagcaa actacttatg aagcttttta tgaaacaatc
     8341 gatacgacca tcatgggcag aaaaacttat caggaagcaa aaaaatattt ggagaccgaa
     8401 gcaatttatc cagagaaaaa gaatttcgtt ttttccaccc acgcacaatt acaattgcca
     8461 gatgctgagg tcgtaaacga agatcctgtt gcttttctaa aaaatctaca aatgacagaa
     8521 ggaaaagata tctgggttgt gggtggcggt cgattgatca aaccgctatt ggaacatcaa
     8581 atgattgatg aatggtatat ccagattacc cctgtattat taggggacgg gattccttta
     8641 ttccaaaaag gaacatatga agcgcgtttt gaaatgctag acacgacacg attcggtgaa
     8701 tttatcgaat tgcattatgt acgaaagtag aagtaaataa aataaaaaag gcaactatat
     8761 gcagaaatgt cctgtacata gttgcctttt ttattaccgt tatgcttttt actctgttaa
     8821 tttgttagaa aaatctagat acgcatctaa aatcgtttgg aagaaactta atgttccttc
     8881 aacgatatta ccattttcat caattagatt cgcaacgttt ccgatatatg cttccggttg
     8941 ctgcaaggtt ggcacattca aaaagacaag tgattggcgt agatggtggt ttgcgccaaa
     9001 gccgccgatc ccacctggtg atgcagtaac gactaaacct ggtttatgat cccaaacact
     9061 gtgtccataa ggtcttgagc ctacatctaa agcatttttc aatactgctg gcacagaacg
     9121 gttgtattca ggagtaacga aaacgacacc gtctagtccc ctcacttcct cacgaaaacg
     9181 agtccattca gcaggagcct gttctggagt ttcgagatcc tcattgtaaa atggcagatc
     9241 atcgattttt acaaatacac tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc
     9301 tacttttttg ttgtaagaat cttttcttaa actaccaata aaaaatccaa ttttttttgt
     9361 catacgattt ttcctccttc aaactatcta taataatctt acaaaagata agtaactgga
     9421 gcaaataaaa acacttactg acaaaagcta catagaagat agattactta tttttaacaa
     9481 aaaagaagtc taaaatcttc tattttctaa aaaaaatcga atttaatcaa gctgtaaaga
     9541 aaaaaagctt tacagggtaa aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg
     9601 aggtggaaat aaataatggc agttaaaatc cgtttaaaac gtatgggttc taaaaagagt
     9661 ccgttttacc gtatcgtcgt agctgattca cgttctcctc gtgatggacg tttcatcgaa
     9721 actgtaggta cttacaatcc tttgaaagac cctgcagaag tagttttaaa agaagactta
     9781 gttcttgact ggttatcaaa aggtgcacaa ccttctgaca ctgtacgtaa catcctttca
     9841 aaagaaggcg ttatgcaaaa acaccacgaa gctaaattct caaagaaata aggtgaccgg
     9901 atatgaaaga tttaagcgac ttagtcttaa caatcgttcg tccgctagtc acttatcctg
     9961 atcaagtcaa gttagacgtc gtagagtcga aagactttta cgagtacaat ctaacagtgg
    10021 ctcctgaaga tattggccgt atcattggaa aacaagggcg tgttgctaaa gcaatccgca
    10081 caatcgttta cggtgttcgg atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg
    10141 ataaggaaaa gtaaataaac gtgctgtact cgaattttcg agtgcagctt ttttgttttt
    10201 tttattaatt gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga
    10261 ggagggagtg aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta
    10321 agcgcttgta gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct
    10381 gaaactgtta ccagtccaga aaaaagtagt accaaaacgg cttccaccac tgaacaaaca
    10441 atggctacct caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca
    10501 aatgttccca tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca
    10561 catacaaagc aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat
    10621 gctgacgaat tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct
    10681 tctagtcaag aaacaatcca agttttgcag ccatttgaga tcgacacaaa tggtcagcaa
    10741 gtggacttag gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt
    10801 gaatggcaag aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat
    10861 cccttactat tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct
    10921 gaacaatttg gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta
    10981 agctggcaag aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg
    11041 aagatggcag tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt
    11101 aactccaagt cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct
    11161 taatacctta aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa
    11221 aaagaaatgg ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca
    11281 aggtatcaaa ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa
    11341 aaaaggaacg gtgctaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa
    11401 aagtcatcgc aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa
    11461 tgatgtggaa aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct
    11521 agctgaaaat gaattctatt accatgagat catcggtctg catgtattag aagatggaac
    11581 tgagttaggt aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca
    11641 acgtccgaaa aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga
    11701 tttagaagct ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg
    11761 atgtattgac tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta
    11821 aggctgtaga taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata
    11881 acaaacatca gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag
    11941 tccagcccat ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa
    12001 gagtaattct tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt
    12061 cacaagaaga acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc
    12121 gcacattagt gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag
    12181 gtgcgatggt catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga
    12241 cttcagctca aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc
    12301 cggcagaata taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga
    12361 ttgaagaatg gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt
    12421 tggaaacact tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag
    12481 aacaagaaaa aacagcagat gaagccaagt gactttttct aaaatcttct ttacagtagg
    12541 ttgataatat gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg
    12601 ctgtgagaaa agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa
    12661 tcgaagaatt aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca
    12721 ctgtgcgtgt tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag
    12781 gtgttgttat caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt
    12841 ctaacggagt gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg
    12901 aagtagttcg ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg
    12961 gaaaagctgc tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
    13021 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
    13081 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
    13141 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
    13201 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
    13261 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
    13321 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
    13381 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
    13441 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
    13501 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
    13561 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
    13621 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
    13681 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
    13741 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
    13801 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
    13861 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
    13921 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
    13981 cattttttaa gtgaatacaa gatcagctaa ttgaacaccg tttattcgtg atgagggaca
    14041 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
    14101 gaaaaatagt agataatccc gtcctaactc ttttattctt ccctcatagc ttccaaattt
    14161 aagcaatact tcaattaatc aagttttatc tttttttttc aataatcgta aaaaaagagt
    14221 attttttatt taaaaataaa atctttgtat taaaggacaa gactaaaata accttagaat
    14281 aaaaagagaa aaa
//
LOCUS       DACCDF010000068        14102 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCDF010000068 DACCDF010000000
VERSION     DACCDF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.071"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            140..460
                     /locus_tag="IVK51_002088"
     CDS             140..460
                     /locus_tag="IVK51_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431660.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IVK51_002089"
     CDS             912..1112
                     /locus_tag="IVK51_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6431661.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IVK51_002090"
     CDS             1259..4048
                     /locus_tag="IVK51_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP6431662.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IVK51_002091"
     CDS             4095..4622
                     /locus_tag="IVK51_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6431663.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IVK51_002092"
     CDS             4643..5833
                     /locus_tag="IVK51_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6431664.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IVK51_002093"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IVK51_002093"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP6431665.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            8224..8898
                     /locus_tag="IVK51_002094"
     CDS             8224..8898
                     /locus_tag="IVK51_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6431666.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8895..9236
                     /locus_tag="IVK51_002095"
     CDS             8895..9236
                     /locus_tag="IVK51_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431667.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            9266..9625
                     /locus_tag="IVK51_002096"
     CDS             9266..9625
                     /locus_tag="IVK51_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP6431668.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9828..10007
                     /locus_tag="IVK51_002097"
                     /pseudo
     CDS             9828..10007
                     /locus_tag="IVK51_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            10149..12230
                     /gene="cadA"
                     /locus_tag="IVK51_002098"
     CDS             10149..12230
                     /gene="cadA"
                     /locus_tag="IVK51_002098"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP6431669.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            12541..14007
                     /locus_tag="IVK51_002099"
     CDS             12541..14007
                     /locus_tag="IVK51_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6431670.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4677 a         2437 c         3016 g         3972 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtggaaatg gctttccaca tttttgcata aatttggctt
     7381 aataccgaga aactggcttt tgaacaccgt ttaaggaaga ggttgtgcgc ctgccgttca
     7441 gctccgagtc acaaggagta atttcaaaaa acagtttttc atgtttgatg actattgtga
     7501 cttgtgaccg aggagttggc cggagagcac cgtttattct aaaaaagaac tgcgaactct
     7561 cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag agaagaaagc
     7621 aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca atcaaacatt
     7681 tacaaagaag gtaattcaaa tggaaatctg atttgatgat gcttctgctg ttgaaataaa
     7741 gcagcatcta gcacctgaaa acaaactaat agtaacattc gaagatggag taggtccgta
     7801 ttttcagcat gcaataatcc atatgtaagt tcaattcacc cttgaaatca tcggaccaga
     7861 taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa gggacattta
     7921 ctagaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag tggtctgatt
     7981 gacggaagct taggtgtgat cgatctctct ggaccaaaca acgaaggaat caaggggaat
     8041 ccggcacgtt aagaaaaaaa tagcataaaa aactagacac ttgatgagcg ttcattcaat
     8101 tgtttgaagg gactaggctg gtgtcatttt ttatacatat attttctctt tcgtcttgat
     8161 agggcttatg ggataatata aggaagaaaa tcatcgtttt attcaaaaag gagtttttga
     8221 caaatgaaaa aacgtatatt atttatcggt gatagcatta cagacgcagg aagagaccga
     8281 gcagaagcaa ctgatctagg taaaggttat ccacttttga ttgccggaaa actaatctcc
     8341 caatatccaa cgatggaatt tgaatgcatg aaccgtggga taggaggaga caaaattgca
     8401 gatctgcaag tacgctggca agaagattgt ttagctttac aacctgacta tgtatcgatt
     8461 ttgatcggaa tcaatgatac ctggcataat actagcgatc aggctatatt cggaactgag
     8521 aaatctgctc agcaatttga atccgtttat cgaaaattac taaatgaact tcaaacagtg
     8581 ggaatctgca acatcatctt gatggagccg tttgtacttc ctataaccga cgaccgtcat
     8641 ggctggcgta aagacttaga ccctaaaatc caaattgttc gtaaacttgc taaggaatat
     8701 ggcgcattgc tggttccttt agatggtata ttcaatgctt tagggattgt ttatggtttt
     8761 tcgaaatata caagagaaga tggggtccac ccaacgattg gcggacatga gatcattgca
     8821 caaagctgga tagatgcaat tgccgcagtc gattttttta gtggagataa aaatgagcga
     8881 aagaaaagtg agaaatgatg gaaatacggt gggcagtaaa agaagatcta cctcaaatca
     8941 atcagctgat tactgatcat ccagaatcat tcgtaaaagc aaaaacggca gctgacctaa
     9001 atgataagaa taaactattg ctcgttgctg taacaaaaga aaaacggatt atcggatttg
     9061 cgcaagcgga cgtgcaacag tcagaagaac gattggatca gcgaccatca ggaaaactaa
     9121 tccaacacac attagtaaca aaagaaaata cggaagaaat ccaaaaaaaa tttgcagaat
     9181 atttgatact ggaagctaaa aaaagaagaa taaaatcatt ttttgcccag ttttaatcaa
     9241 aaacagtaat agaaggagaa atacaatgag ttgtttagga aatgtcatct ggtttatatt
     9301 cggcggacta atcggtggtc ttagctggtt agcagcgggg atactttggt gtatcacgat
     9361 cataggtatc ccaatcggta tgcaatgttt taagatcgcc tccctttctt ttgcgccttt
     9421 tggcaaagaa gtaatttatc accagtctgg catgtcattg attgttaata tcatctggct
     9481 gatcgtttca ggcctaccaa ttgctctcgc acatgtcgtc agcggcatcc ttctatgtat
     9541 cacgatcata gggatacctt ttgccaagca atctttcaaa cttgctcgat tggcgttgat
     9601 gccattcggt gcagaaattg tataaaagag aataataaag gttagataaa gcatagagtc
     9661 aggtgggata catgtatttc acctgactct atgcgttctc ctgtataagt tcttccatgt
     9721 gacttttacg tcttttattt atatggggtt actgtttatg gaaagttgaa ataattttca
     9781 ttaaagaact ctttctatat ttttgaaagc gtagtggagc ttcgaatatc tgggaaggac
     9841 attttgcagg gattgaaaaa agatattgtc ctatcgaaaa attaatgaat ttagacaaat
     9901 tgccagcaac aggcttcaaa cttagttgct atcctgtcaa gatcaagaag gcaagtgcgg
     9961 gttggataag agcagtggca atgatcgaaa agaaaaagaa agaataagcc aaaacatctg
    10021 tatggaagaa attttccgat gcagatgttt ttcatttttt ctttcggata ccttgacata
    10081 tgaataaccg ttcatttata attagttatg aagagatatt catatgttga ggcgaggagg
    10141 tcattaaaat gaaaagttac agattagaag gtttagattg tgcgaactgt gcaatgaaaa
    10201 tcgaaaaagg agttcagaag atcaatggag tcaaggaagc aacggtaaac ttcacttctg
    10261 gaaaactaac gattgacgca gaggaagatc atttggctac gattgaacaa gaaataaaaa
    10321 aagtagtgaa ggaattagaa ccagatgtca aagtaacaga aatagataag gaaaaagtat
    10381 ctgaacatgg aaatgaaaaa gagagaaata cgctgttccg gattttattt tcccttgcag
    10441 gtatcgcttt attgctatta tttgatttta atgaaccaat tcgcttgata gggtatttat
    10501 tgatttatct gctgatagga tatgatgtag tgaagaaggc agtgatgaat atcgtcaaag
    10561 gaaaaatctt tgatgagaat tttcttatgt ctgttgctac gattggcgct atgttgatcg
    10621 gtgaatatcc agaagccgta gcagtgatgc tattttatca aatcggcgag tatttccaag
    10681 gattggcagt cagtcattca agaaaatcga ttcgcgaatt gatggcgatc cgtccagaaa
    10741 cagctcacgt gcagacgacg gaaggattga tgaccgtgaa ccctgaagac gtgcagatcg
    10801 gtcagtttgt acttgtgaag cctggtgaac gtgtaccgtt agatggaacg atcatcgaag
    10861 gagaatcatt agtcgatacg tcagcactta caggagaatc agtccctaaa tctgtatatg
    10921 ttggtgaaac agtattaagt ggatttatca ataaaaataa accattttta gttcaagtag
    10981 aaaaatctta tgaaaattct actatcagca aattattaga attagtagaa aatgccagca
    11041 gtaaaaaagc accagctgaa aatttcatta ctaaattcgc gcgctattac acaccagtcg
    11101 ttgtaggatt ggccgtttta ttagctgttt tacctccact tgttgtttct ggagcggcgt
    11161 tttctgaatg gatctatcga gcattgacat ttttagtgat ctcatgtcca tgtgcattag
    11221 tgatctccgt accccttagt ttctttggcg gtatcggagg agcaagtaaa atcggtgtat
    11281 tagtaaaagg ttcaaattac ttggagttgc ttgcacaaac tgagacagtg gtattcgata
    11341 aaaccggcac attaacgaaa ggtgactttt cgatccagac gatcgataca aaaatcgatc
    11401 caaagatatt catgcaatat gttgcgagtg cagaacagtt ttctacacat ccgattgctc
    11461 aatctgtttt agaaggctac gcaggtccgc ttttaccaac agctaatatc caagaatttg
    11521 caggtgaagg gatcttagct gaagttgatg gcaaacaagt attagcaggt aatcacaaat
    11581 tgatggaaag atttgaaatc tcttttcctt caagtcaaga aatcggtaca ttgctttatc
    11641 tagcgatcga tcaaagttac agtggttatc ttgttattgc agataccttg aaagaagatg
    11701 ccgtagacgc attggttcaa ttaaaaaagg ctggtgtgaa gaacacagtc atgttgacgg
    11761 gcgattcgaa aaaaattgct gatcatatcg ggaagcaagt aggcgtggac aagatatata
    11821 gcgaattgct tccagaagat aaagtgcagc ggttagagga aatcttacaa agaaacaata
    11881 aaaagaccgc atttgtcgga gatggaatca acgatgcacc ggtgctagca agagcagatg
    11941 taggaatcgc aatgggtgga ttgggaagcg atgctgcaat tgaagcagca gatgtagtca
    12001 tcatgaatga tcaaccatcg aagattgcag aagcaatcca tttagcaaag aaaacattga
    12061 aaatcgtcaa acagaacatt gtctttgcta ttgggataaa aattttggta cttgccttag
    12121 gtgcttttgg ttttgcatca atgggagatg cagtatttgc agatgttggg gtgacagtat
    12181 tagctgtttt gaatgccatg cgaagcttgc atgtaaaaaa taaacgttga tcaatgaaga
    12241 ggacgaatga acgtcctctt ttttttatat ccttttctta atgaaaaatg taaagaaaat
    12301 agataggatt ataaaataac taatgttttt aattttgcag aaaattcaaa atagcttgaa
    12361 attcaataag gtaaatgaaa taaaagaaaa taacttattt ttttgatgga aaaaatatga
    12421 aaaaacgatg tgtattaaat aaaagaatac acacataaaa taacactgtc ttgtaagttt
    12481 gttaaaaatt gaatatgatt acatctggat aacaaatttt tatcataaag gagagaacga
    12541 atgaacaaca aacaattacg ctggtttact gttgcgttaa ttgcatttaa catggtttgg
    12601 ggcatgggga atgtagttaa taactatgct cagcaaggag tatcagttgt cacctcttgg
    12661 ctattgattt tggccattta ttttattcca tacgcattga tcgttggaca attagggtca
    12721 gccttcaagg atagtaaagg aggcgtcagt tcatgggtcg aaaatacgac atctaataaa
    12781 cgactggcat actatgccgc ttggacttac tgggtcgtcc atattcctta tttggcacaa
    12841 aagcctcaag caatcctgat tgctggcggt tgggcagtcc aaggaaatgg aaatatcgta
    12901 aatacgatgt cagtacaaat ggtagcagtt atttcactta ttatatttct tgccttttta
    12961 tatttatcaa caaaaggact ttctactttg aaagtgattg gcggactggc cggaacagcg
    13021 atgttcgtca tgtcgatctt atttattctt ttagctgtaa cggcaccgtt catcaatcca
    13081 acgatggaat ttgccacacc acatatgaat caagtgaaaa catatattcc aacatttgat
    13141 ttcagttatt tcacaaccgt ttcgatgcta gtatttgcag ttggaggagc ggaaaaaata
    13201 tccccttatg tcaactctac aagaaatcct gcaaaggaat ttcctaaagg gatgatcttc
    13261 ttagcagcaa tggtagggat ttgtgctgta ttaggctcag tcgcaatggg tatgctgttt
    13321 gctagcgata atattcctaa agacttgatg gcaaatgggg catacagtgc ttttcaaatt
    13381 ttaggtaatt actatggtat cggtaactca ttgatgatca tttacgcatt gacaaacatg
    13441 attggacaaa tctccgcatt agccttttca attgatgcac cactaaaaat cttgttagca
    13501 gatgcggatc cagaatttgt accaaactgg ctacgtaaac gtaatcgtaa aggtacattg
    13561 gtgaatggtt atcttctgac aggtatacta gtaggaatca ttattttgtt gccgatcttt
    13621 ggtatcaaag atatgaatga attagtaaaa tggttgacga acttgaactc agttgtcatg
    13681 ccaatgcgtt acctatgggt gttcttggca tatatgatgt tgaataaagc ttataagaaa
    13741 ttcaattctg aatataaatt tgttaaaaat ccaaaaatcg gttttatctt tggtgcttgg
    13801 tgctttgcct tcactgcgtt tgcatgtatc ctaggaatgg tgccaaaagt agattatact
    13861 gctgatccta aagcatggtg gttccaatta ttcacgaata tcatcacacc aattgtcttg
    13921 atcttattag gtatgatctt gccagcaatt gctcgtcgag acaaaaacaa agaaatcact
    13981 gaaccagaaa cagtggttca gccttaaaat agaaatactt gttacaagag gttgtgacag
    14041 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    14101 aa
//
LOCUS       DACCDF010000069        13836 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCDF010000069 DACCDF010000000
VERSION     DACCDF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.072"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(24..1043)
                     /locus_tag="IVK51_002100"
     CDS             complement(24..1043)
                     /locus_tag="IVK51_002100"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP6431671.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(1676..2680)
                     /locus_tag="IVK51_002101"
     CDS             complement(1676..2680)
                     /locus_tag="IVK51_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6431672.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(2693..3742)
                     /locus_tag="IVK51_002102"
     CDS             complement(2693..3742)
                     /locus_tag="IVK51_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6431673.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(3759..4580)
                     /locus_tag="IVK51_002103"
     CDS             complement(3759..4580)
                     /locus_tag="IVK51_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6431674.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(4567..5346)
                     /locus_tag="IVK51_002104"
     CDS             complement(4567..5346)
                     /locus_tag="IVK51_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6431675.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(5363..5833)
                     /locus_tag="IVK51_002105"
     CDS             complement(5363..5833)
                     /locus_tag="IVK51_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6431676.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(5845..6264)
                     /locus_tag="IVK51_002106"
     CDS             complement(5845..6264)
                     /locus_tag="IVK51_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP6431677.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(6345..9113)
                     /locus_tag="IVK51_002107"
     CDS             complement(6345..9113)
                     /locus_tag="IVK51_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6431678.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            9313..9537
                     /locus_tag="IVK51_002108"
     CDS             9313..9537
                     /locus_tag="IVK51_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431679.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            10316..12031
                     /locus_tag="IVK51_002109"
     CDS             10316..12031
                     /locus_tag="IVK51_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431680.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            12034..13797
                     /locus_tag="IVK51_002110"
     CDS             12034..13797
                     /locus_tag="IVK51_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431681.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
BASE COUNT         4065 a         2831 c         2402 g         4538 t
ORIGIN      
        1 acgattaact attgatcatc tatttaacga agagaaaatt cattctccat ttttccgtca
       61 cgtaacatga tttcttttgc tgcttttttg atatcttctc cattatacaa aacattatag
      121 attgtttgag taattggcat ctcaacacct agctgttgag ctagctctac tgctgctttt
      181 gtcgttgcca caccctcaac gaccattccc atgttttcta gaacttcttc cagcttatgt
      241 ccctgtccta acaagttacc ggcacgccaa ttgcgagaat gaacacttgt acaagtaacg
      301 atcaagtccc ccacaccact caaaccgata aatgtcagag gattggcacc catcgcgaca
      361 ccaagacggc tgatttctgc caatcctcga gtcatgattg ctgcttttgc atcatctccg
      421 aatcctaatc catggatagc gccggcacca attgcaatga tatttttcag tgctgcacca
      481 gtttctacgc caatcacatc attatttgta taaatcctga aataatcatt catgaacagt
      541 ttttgtacat aggctgctgc tttttgatca atacttgcag ctgtaattgt tgtgatatca
      601 tgtactgcca cttcctcagc atggctagga ccagataata caacaattgc ctgtcttttt
      661 tcttctggaa tttcttcagc caaaacttct gagattcgtt tatgcgtatc ttgttctagt
      721 cctttacttg catgtataat gattggctta gtattcattt taccaataag ttcctgtgcg
      781 actgaacgaa tcgcttttgt tggaacaaca aataaaatag catctgcgtt atccactgct
      841 tctgccaatt ctttatatcc gataatcgat tctgggatcg tcaaatcagg taaaaaatgc
      901 tgatttgtat gatacttatt gatttcatca atctgttcag gtacatttcc ccaaatacgg
      961 acatcatgcc cgttttctgc caatgtttgt gctaaagcag tgccccaaga tccaggacca
     1021 agaactgcga ttttttgttt catggatatt cctccaaata aataattgat aatttccatt
     1081 atagtataca tttcttaaag ttgggactaa tttccattgt ttttaagtgt ttgtttactt
     1141 cttttatcgt ttttacttga aataatcaat gatatgttac aatagtctac attttatttt
     1201 tacactcgtt atgctttgct tgttcctaag aggaagcagt tttgacaatg ttactaccag
     1261 cattactatt aggaggttag gaaacttctc ctagtgagta ttgatttaaa atacataaga
     1321 gacagattga acatttctaa atctgtcagg tcctgcaacg gacagattgt ctctgttgac
     1381 ggttgacata ggaacttttg ttttagtcaa cattctggat acaaaacgct atgtaaactg
     1441 gcgcaactcg ttgtacttgg acttttacta cggatccatg caacaactaa atagtactat
     1501 tgcataacaa aaaatggttg gcacccacat gtcccattaa acagattata agttgaactg
     1561 tagataatgc aaaaaatgtg ttttataacg taattgatgc caatatttta aaaaaataaa
     1621 gggagcagtt atcgctgctc tctttatttt ttatattctt tttatccagt ctatcctaat
     1681 aatcaaactg acgatagtag cggcgtatct ctaatggatg gcagttcaac tgctccacat
     1741 gagcatcgat gcgttgtgtc actacatgca tcaataagaa agacaaaaca cgtccgcgat
     1801 atttttcact gataccttct actggatagt cttttgtgtc tatcaatgta taattgctgc
     1861 aaatttttgg caaaagttta gccacacgtt cagataacac tctctgttca tcttcgccta
     1921 aaaataatgt gactggggtt gtttcttcta cgatctctaa cgtcccgtga agaaactcgg
     1981 cagcatggat agatttcgat ggaagccagc tttgttcttc ccagtaacac atggcatatg
     2041 aataaacagc tccccattga ttccctgctc caataaaata atgcatacta tcacgtcgat
     2101 gtttttctgc aaatgataag ccaaatgcat ctgctttttt ctctgcttcc actaaccctg
     2161 ttggtaaata acgttcgagc tgttcatagt attctgaata agcttcgaat tctccattca
     2221 aatacatcaa tcgatctgct gccataaaaa atttgatttg ttctgttcca ggagctggat
     2281 aagtgaccac aaaatcgcat agctgagaca acttactgtt cgctttatcg ataaatccaa
     2341 tcagtgtcgc tcctactttc ttgatttgtt ctactgcttt cactacttct tgagttgtac
     2401 cggtaacaga ggacaaaata acgatagaat cttttgttaa tcttttgttt ccagtagtgt
     2461 aatattctgc agcatgctgt acaaagacag gcaagttgct tttcccattc atataagtga
     2521 cagcttgcat cgaggaagca tacgttccac cgattcctag ataatagaca gcgtcaaatt
     2581 tttccgcata aagtttatcg atgatttctt ctacttgcgg acgtaaagct agtgcgcctt
     2641 ttattgcttt gatttgttct tcttcattga atttcagcat gatttccacc tcttataatt
     2701 tacttttcaa gactgtatca aagtcttcaa aaatcttttt gttcaagtca atacctttag
     2761 ctactgcggt tttataagcg aatagttgaa acggaacgac aagcgctaat acagacagat
     2821 attcatcaca ttctattgct aatccaagtg ttttctcgtt tcttgctttt tttgtggtaa
     2881 tggtgagagt ctctccaacg taccgactca tatatctttg caacttttgc gaacgttcct
     2941 tatttacact ttccccttca ataaagaata gcaaatgcga agcatctgcc tccaggtatg
     3001 ggccgtgcat ataagcttct aactcaaatc cttgtgacgg aacgcggatt gtttcagtca
     3061 atttcgtctc tgcttcttta gctgttcccc agtttggacc atatccaatg gtgacgaaac
     3121 gctgtgctaa acgaaataaa ctttgatatc catcaaaaaa ttcttccgtt ttttgaataa
     3181 tcgctggtaa atggttgatt attttgcgca actgttgcat gtagtcttgt tctatttttt
     3241 tgctgatttt atttttactt attccaattc ctatccctaa gagtagtaac tgtaaaacag
     3301 tcgcagaata gccttttgta acgaagccta ctgattctac cccggcacct aaatcaataa
     3361 tttgtgcagc ttctaaagca agtggacttt gcacgtcatt tgtgatagca acaacaggta
     3421 acccttgttt ttgtatcatt tttactgctt caacagtaga agcacttttc ccgctttgtg
     3481 acacagcaat cacagtatcg attgaaggag aaagttttcc ataattgaaa aaataaaaag
     3541 gttcttgaat atcgacagta agatcaccat aagattccaa tgctggcttc gctgctaaac
     3601 aagcgttata agatgaaccc gttgctaaga tcagtaaatt cactgttttt ttcatatgtt
     3661 ctcttgtcga aaaatcattc tttttcaaga tcgtacttag tacttccttt tcttcataga
     3721 tataatcttg catcgtaaac attttctctg ctcctttttt aaaaaatccc tatccacgaa
     3781 ccaaaaattc caaagaaagc tagtccgatc aagatcgtta atggtttcac ttctttcttc
     3841 aatagataaa acactatgcc aaatgctcca agacataaga tatttggaac gatatcatca
     3901 aaaatacttt gaacagtcac agcattttct cctgaaccga tttttaaagg aatattgatg
     3961 gtgatcatcg tggcagtcat tgctccaacg accatcagtc caataataga cgcgcctaat
     4021 gtcaaacttt ccatcatacc gtttgcctgg atctttttca aaaaacccgt tcctaagcga
     4081 tatccccaac ctgttgctag atagcgaaac agaatgtgcg gaatattgaa aatcaaaatg
     4141 aacaagatag ggcctaaaat atttccttgc atggctaaag aggtacccac acctgtggca
     4201 atcaatcgca aagttcccca aaagaaagaa tctcctaacc ctgcaagagg tcccatcaaa
     4261 gatgttttga tactatcgat cgcagtcaca tcaaattcag gatcatcctt attttcttcc
     4321 tccattgcag ccgtgattcc taaaatcagt gtaacgatat gtggggtcgt gttaaaaaac
     4381 tctagatgcc gtttcaaagc tgctgccatt tcttctttcg actgatataa ctttttcaaa
     4441 atcggaatca ttgcataaac ataagccata ttcatctgac gttcataatt ccaagaaaac
     4501 tccatttgaa aagaacgcca aaatacgtga tttagctctt ttttcgttat tttgctgttt
     4561 ttgatctcag aattcttcat cttcttctac ctcccctgca gttgtttgaa gtggtagttg
     4621 acggacattc ataatattga ctaccacaac tgctaaaatc gcaccaaaaa tagctactcc
     4681 ggtcacagga atctctaagt aagaagctat agcaaaacct aagaaaaaat atggtgcaac
     4741 ttgtttgttg atcagcaaac gagcaagcat cgcaaaacca agagctggaa tcaaccctgt
     4801 agctactgac aacccctttt gaacgaagtc aggaatcata tttaatagat ttccgatcgt
     4861 attgcttccc accatataag aaaccattac cacaacacca agcatcaagg aaagaccgat
     4921 accggcaagc aagtgcatat gctcgactcc tttataattt ccttcttctg cataattgtc
     4981 tgctttatgg ttcataattg gaatcaaaag ccctaagtaa atatttttca atatcaatgt
     5041 caacgtagca atcggcaaac ctagtaaaag cgcggtttct gtaccggctc cagctgtaat
     5101 agcaaaagcc gtccctaaaa tcccaccggt cacaacatca ggtggaatcg aagcacctac
     5161 tgagaacgat ccgataaatg ctaactctaa agttgcgccc ataatgatcc cagtttttac
     5221 atcacctaac acgattcctg taaacagccc cgtcacaatc ggacgagaaa gcaacgatgt
     5281 ccctagtgca tattctgatt gtgcgacaaa tgcaacaatt cctaataaga ctgcttgtac
     5341 aatcattgag gttcttcctt tcttacaaaa cttctcttac atccactttt ttatctgctg
     5401 gtacttgacg gatttctact tctatttgtt tgtcaattaa tcgtttcaag cgttcctcct
     5461 cttctggtaa aatatttacc gcttttccga tgctgcgagt accaggtttt gccttgatcc
     5521 ctcctaagtt gatggttttg atttctggat acgcttctgc cagcttatat gcatcctcga
     5581 tcgattccac tacgatgaat aacttatatt tatctgtcac cccgcttttc aacgcggcaa
     5641 tcgagtcatc aattgatttg atcactagtt tgactccttg tggcttggct aatttgatcg
     5701 ttgtctttcg tatctcattc gtaggtacat catcattagc aatcaaaata cagtctgcgc
     5761 ctattgtttg tgtccatgaa aaagctactt gtccgtgtaa taaccgatgg tcgactcttg
     5821 tcaatacaat catttttctt cctcctaaaa ttcgtcttct tctattttct gattgagccg
     5881 ctcgttacaa aactggatca ttttttttga atcagttagc acctgctgga tcaaaacagt
     5941 tgtctctact tcagcgtcca accgagatac tagctcaatc aatagtggca aattcaatcc
     6001 ggcaacgaga tagacatgat cccgattgat atattgtaag aattcgttgt tgacacttcc
     6061 gccaaaaaga tccgtcagaa caatgatttc tttatctttg tacctcataa aaagctgcct
     6121 tactgtttcc gctaaatcaa aattctcatc agtatagcaa tcgaatacag taatagaatg
     6181 atttttccca tagatcagct ctaatgaatc taatatccct gaagcaaatt ttccatggga
     6241 agctaagata atttctctat ccactttttc atctcctctt ttcacttaca catactaaaa
     6301 aagcaagttg catgccaaaa ttagtggcaa caacttgctt atgttcaaaa ttcttcttct
     6361 atcgagagaa actctgtatc ttcactcaaa atatcataga tataggcaat ctcagaattg
     6421 gggatcatga cactataatg ctcttcaatg acactaaacg cagatttaat agcagataac
     6481 ccttcttttt gacactgctt ccatttctct aatccttgat aggtttcgat tggcatttgt
     6541 cggatcaatc gttcaatgag acagctcaca tggacataca acgctaattt tttgggattg
     6601 ctgatgcttc gattcaactg cacctctaat tctctcataa aatgatcgat ttctcccatc
     6661 accttctctg tatctagaat agtcacggaa tccagtactt tttccaatga aaaattacga
     6721 atgatgcgct gattgaatat ttccttctct gaagcattca tagtagtgtc caaccattca
     6781 gctagtacag acatcttttc ccccgagatg aggtcttcta atgaaagata gtcgatattg
     6841 gtgatatcag gatctgtggt gccaatcatt cctacgactt cgtatatgga aaataatggg
     6901 tcactgtttt tcttatcttt taattgctga tattcatatg ggatgatttt caactgacaa
     6961 gccgtaggta agcttttttc tagcagttca cttaagtgga ccgccgtacc gattccagtg
     7021 aagcaagtag taatgagtgc tttgggtttg ttttcctctg gatagagcag tcgccattct
     7081 ggctggatct gtcgaacagt ctcttctgct atctcttcta atgatacatt tttttgcagg
     7141 cattccccta ctgtgattgc taaaggtgtc gtcacattat tcaaaatcaa aagcggtacg
     7201 gataactgtt ttttaaaaaa ctgatggatt tctttcagag atcccatgtc tactaatatg
     7261 atcaatccat ttgaaacatc gctgttttcg caataatcaa ggatttctga agcaatctgc
     7321 tgtggtgtga catctagtgg catatcaaat gattcaaata gatcctcttg taataaacga
     7381 tttactacat ttgccatact gctagcagtt gcataaccgt gagcagcaat catcgctttt
     7441 ggcagtcgtt tttcttttga ccatttcgct ttttttagat agatcgtcag taaaatccga
     7501 tccatacttg tgtcttcaat atctaatttg atcttactta attccagaat tcgttggaca
     7561 tagtgataac tagatggata agactgagaa acttgttgtt ctaattcttt gattagttgg
     7621 ataatatcat gttcttccgg taaccatttg atagtgcttc gctgatagag atagtaactc
     7681 aaggcataga cactgtttcc atcgaatttg atttgatagg cagtttccat ttgtcgaaag
     7741 gtctctcgga tagattgtgt ccaataaacc agcagctcgt gtttttcttg tctactggtc
     7801 tcaaacagca aaaaatcaaa taattgaatc acttcttgct ttaattttcc ttcgcaagaa
     7861 tatagcagac agttattttt cttgaaatca attaggattt gctcaaaact tttaacgatt
     7921 cgctgctgtt cgggctgttg cttctccatt aattgctcta aggtctggtc ttgcgtaata
     7981 tagattcctt gctgtgctgt atttttagta ataggctgcg caagtagttc tttttgcaaa
     8041 tgatggatcg tgacatggat ttcaggctgt tcacgctgtt cagcatatgc ttttgctgct
     8101 gaaactttga cgatattttt cagctctcct atgtttccgg ataatttctg acttgttaaa
     8161 agtgacaaag ctggtccact gactgacaaa gggctcccca tttttctttg ttcttcgaga
     8221 aaaaaagagt aaatcagttc tagccgttca ttacgactac gctgattaag tgcaggtagt
     8281 tcgattcgga taggtattct tcgaatgaaa gttgttaaga aatgactagt aatctcttca
     8341 gtcgtagcaa aaaagagacg tgttttgatc cggcgtgaca aattaggttc tcccatacga
     8401 taaatcacac cttgatcaag ataggtgaac agtttttcct gaccttctgg tgttaagcga
     8461 tgtacctcgt ctaaaaacaa gatcccacca tctgctgctt caaaagcccc ttttttattt
     8521 tcttctgcac cagtgtacgc tcccttaaca tgtccaaaca aattgctagt caaaagttca
     8581 ggattatctg catattgtgc acaattaaaa gtgatgaatg gtgcagaatc ttctagcaga
     8641 tcatgaagca gacaatactg atagaccaaa ttaactaaat aacttttacc tgtgccactt
     8701 tcgcctgtaa tcaaaagtgg taatccgcca tctggatagt aaagagctgt tttgatttgt
     8761 tcgatacttt ctttcaaact ggcgtcgtat ccgatcataa aagagaataa gtcttttggt
     8821 tgcgcgaaaa aaggctgttc ctctttgaga tcatcgatag aaaaataaaa attcgtgcgt
     8881 aacttaaaaa attgtttttc aaaagcttct ttgtggaaat aaatcactgg tcgagaattg
     8941 attttgacta gtagttgctc atcatttaac tggttcaaat aatgactgac tgtgtttctt
     9001 tttaccccaa aatgatcagc taacctttgt gctgtaaaga cctcgttcat atgttctaaa
     9061 tcgaaaaagg ctgtctggtt ctctaagtaa cataaaattt ccttcttcaa caacttattc
     9121 acccctcctc catcatagaa aacggattca ttttgtcaat aaaatcttta ataaagaatt
     9181 gaaaaatata tcttattata gacagcagcc tacttatccc ttataatttt tattctaaaa
     9241 aacgtacagt tccttccagt ttttatactg gaatttcaac atatgaaaga ataaggaggc
     9301 tatgactatt caatgaaatc ggataaatat aaaaacagga aacatacttc ctggaaaact
     9361 tttcgagaaa tcatcggatc ttatctcgct gtagttgtat ttttgccttt gctttatatg
     9421 gctatgacag aacaaactcg ttttattggc gtagatacgc aatatatagc cgaaacagga
     9481 tttttattca tctttatctt gatttttgcg ttctataaat ccttccatac aaaataagat
     9541 tcacagattt caactttcct gtctgttttt agctattaca aatccttata tgtggtataa
     9601 caacaattta ttggcataat gaggaaaaac gagttatgat gctttcagag agtaaagagg
     9661 tactgttgtc accatccttt ttattgatta cgtacaccac tcttagtttt ttcccattga
     9721 ttacgaaaac aaggtagaaa cattgatgtt cctaccttgt tttctttttt tattttcaga
     9781 tcaaaatctc aagtagcttt tgacttgatg tcgggaggtt gtcttcgttc catcaaacct
     9841 gatagaatct gcgccaaacc atagttttct ccactattat ttattcctct aagatagtac
     9901 ccacaataaa cgaataaata tttttctcat tttcatactc cttctttgaa aatcaattag
     9961 atttctctca acccactttt ttacaaaaat tacgtagcaa aaaagattgc aagaacaaat
    10021 taaaaaagaa gtaaatagag tttcatctat aaacatttac ttgatatatt ttttatgcag
    10081 aattcattta tagaaaaatt aagtaaaata gacgttccta tgaatgaaga gactggcatg
    10141 ttttctcatt ttcttttttt taaaaaaaat agtctattca atttctaaag cttcttcata
    10201 ataaaacttt catttatgaa aaggcttgtt ctcttttcat tctaaatttc ctaagctaca
    10261 atgtggtagt acttgttttt tcaattgtgg acaaataaat aataggagga atcatatgat
    10321 tcagttatta cgttatgcaa aagactatcg taagcaaatc attttgggcc cgttcttcaa
    10381 atttcttgaa gctgtcttcg aacttgtcct gcctttaatg atggcttcct tgatcgataa
    10441 tggactaaaa atgaatgacc gaggaaaaat cattgaaatg ggcttatgga tggtcgctat
    10501 gtctgtgatt ggtctgattt gtgctatcat ttgccaatac tatgcttcag tagcttcaca
    10561 aggatttggt acagagttac gtaatcagct aatcaaaaaa atcaatactt tctctcacaa
    10621 cgagttgaat cattttggaa cagatacctt gatcacccga atgacaaacg acatcaatca
    10681 gcttcagcta gctttagcaa tggtcattcg tttattgatc agagcaccgt ttttaagtat
    10741 cgggtcagtg gtcatggctt ttgtgatcga ctgggaagta ggtctgtttt tcttagcatt
    10801 attgcctatt ttctcaatca ttttattttt tatcatcaaa aaaaccgttc ctctctacca
    10861 aaaagtgcaa gaaaaactag atcagctcaa tgaacacgtt agtcagaatc ttagcggtgt
    10921 tcgtgtcatc cgcgcatttg cgcagacaaa aaaagaaaca gagaagttcg ataaaagcac
    10981 aaatgatcta gcgaacaatt atttacgtgt ttccaatatc tctgccctat tatcacctgc
    11041 tacaaccttg atcatgaatg tcgggatcat ctgtttgctg acagttggcg ggatcaaagt
    11101 aaatattggt agcttgcaac aaggacaagt tcttgctttg atcaattata tgaatcaaat
    11161 gcttttggca ctgatcgttg tttcaaacct cgtcgtgatc ttcacacgtg ccgaagcttc
    11221 aggaaatcgg gtgaaagagg tgctagacac agaaaattcg attcttgaag ctgaggtcag
    11281 tactactccc gatttttctt ctgaagcaat tatcgctttt gaccatgttg acttccgtta
    11341 tactcctgat tctggtcttt ctctccaaaa tattacattt cagctgaagc gaaatacagt
    11401 ccttggaatc attggtccaa ccggaagcgg gaaaacgact ttgacacaat tgattccgcg
    11461 tttctatgat gtcagttccg gtacagttat aatggacgga atggatgttc gttcttggca
    11521 gctggatcat ctccgtcagc aaattgctct tgttccccaa acttctgtac tatttaccgg
    11581 gactattcgt gaaaaccttc aatggggtaa gaaagacgcc acagaaaaag aatgttggga
    11641 agctctgaaa atcgctcagg cagaagaatt tgtccgtcag cttccaaatg gtttagatac
    11701 aaaagtcatg gaaaatggga aaaatttctc tggcggacaa aaacagcgac tgacgattgc
    11761 aagagctttg attgccaagc cagcattact gattttagat gactctttaa gtgcgttgga
    11821 ttatcaaaca gacttggatt tgcgtaaggc gctacagact tatcttggca gtacagtgat
    11881 tattatctcc caacgtgtcc gctctattca agaagcacat catatcttag tgatggatca
    11941 aggtaagatt gctgctcagg gtacccatga agaattactt gctcaatcag cagaatatca
    12001 ggaaatcgtc gcatcacagg aggaagaata gttatgaaag taaaaaaatt atccttgcag
    12061 acgctccgcc gattcattcc ttaccttact gcctatgcaa aagacatttg gctagctgtg
    12121 atactaggaa tcatcaatgg gattgcaaca gttgtgatga cgttacaaat tggtcaaagt
    12181 atcgatcaaa tgattggaga aggacaggtc cgcttcaatc gactgttcca tttacttatt
    12241 ctattcggtg gtatcgtttt aatcaacgta gtcagtcaat ggctcatcca agtgttaagc
    12301 aatcggttag cttacttatc cgttgcaaaa ttaagaaaag atgcatttgc tcatctgaat
    12361 cgattgccac ttcgttatta tgatcaaaac tcccacggaa atatcgtcag tcgatttaca
    12421 aatgatatgg acaatatttc cattgcaatt tctgcggcct tcaatcaatt attttctgga
    12481 gtggcaatca tcattctttc gtttattttt atgatgcgtc taagtccact tctgacattg
    12541 gtcgttcttt gttctacacc aattattttc cttgtgagtt ggatcgttgc tcgtgcttca
    12601 caaacaaatt tcgatagaca gcaaaggatt attggggata tctcgggctt tgtgaccgaa
    12661 cgaatcggta accagaaaat cgtgaaagct tttcagcaag aagaagctag tcaacagcaa
    12721 tttgaactgc taaatgctga tctatatgaa aaaggacaac gggcacaatt ttcttcttcc
    12781 ttgacaaacc cgtcttctcg ttttgtagat caccttgctt atttatcgat tggtttagtt
    12841 ggcggcctat tggcattgaa gagtggttcg tcaatcactg ttggtgtgat ttccagcttt
    12901 acgatctact caagtcagtt ttctaaacca tttatcgaac tttctggaat cacgacacaa
    12961 ctgcaaaccg ctttttctgg tctagaacga gcatttgatt tgatcgatca gccagaagaa
    13021 aagcctgatt tgccagatgc ctatgccttg gaacccaaaa atatcaaagg aaaagtctct
    13081 tttgatcatg tttctttttc ttatgacccc aagcagcgtt tgatcgaaga tttcaatttt
    13141 actgcttatc caggtcaaac cattgctata gtaggaaaaa caggggccgg aaaatccaca
    13201 cttgttaatt tgctgatgcg tttttatgac acaacaaaag gaacaattac agtcgatgac
    13261 catgacatcc gctctatcca acgtgataca ttacgtaaaa gttttggaat ggtgttacaa
    13321 gatacttggc tatttgacag tacattgcga gaaaatcttc gttttgggaa tccggaagca
    13381 agtgacgcag aaatcaatga tgccctaaaa gcctcttata tgtatgaatt tgtagaacgt
    13441 ttgccggaaa aattagatac taagattggc ggtcaagggt taaagatatc agatggacaa
    13501 cgccaactgt tgacgattgc tcgaacgatg atcagtaatc cccctatgtt gatcttagat
    13561 gaagctacca gctcagtcga tacattgaca gagaaaaaaa tccaacaagc cttcttagca
    13621 atgatggaag gaaaaaccag ttttgtgatt gcccatcgcc tgtccacaat caaaaatgcg
    13681 gaccagatat tagtgatgga tcagggagct attgtcgaaa tgggcaccca tgatgagctg
    13741 cttcaaaaag atggttatta ccgacggctc tatcaagcac aattcacagc acaataaaaa
    13801 caagcagtct tctgggatgg gaagactgct tgtttt
//
LOCUS       DACCDF010000070        13811 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCDF010000070 DACCDF010000000
VERSION     DACCDF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.073"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            131..505
                     /locus_tag="IVK51_002111"
     CDS             131..505
                     /locus_tag="IVK51_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6431682.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IVK51_002112"
     CDS             complement(619..1248)
                     /locus_tag="IVK51_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP6431683.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLPLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IVK51_002113"
     tRNA            complement(1382..1454)
                     /locus_tag="IVK51_002113"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IVK51_002114"
     tRNA            1668..1741
                     /locus_tag="IVK51_002114"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IVK51_002115"
     CDS             1696..1845
                     /locus_tag="IVK51_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431684.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3560
                     /locus_tag="IVK51_002116"
                     /pseudo
     CDS             2338..3560
                     /locus_tag="IVK51_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            3762..4595
                     /locus_tag="IVK51_002117"
     CDS             3762..4595
                     /locus_tag="IVK51_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP6431685.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4611..5348
                     /locus_tag="IVK51_002118"
     CDS             4611..5348
                     /locus_tag="IVK51_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP6431686.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5503..6471)
                     /locus_tag="IVK51_002119"
     CDS             complement(5503..6471)
                     /locus_tag="IVK51_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP6431687.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6703..7536
                     /locus_tag="IVK51_002120"
     CDS             6703..7536
                     /locus_tag="IVK51_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP6431688.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7570..8409
                     /locus_tag="IVK51_002121"
     CDS             7570..8409
                     /locus_tag="IVK51_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6431689.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8435..8995
                     /locus_tag="IVK51_002122"
     CDS             8435..8995
                     /locus_tag="IVK51_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP6431690.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9194..10915
                     /locus_tag="IVK51_002123"
     CDS             9194..10915
                     /locus_tag="IVK51_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP6431691.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11080..12222
                     /locus_tag="IVK51_002124"
     CDS             11080..12222
                     /locus_tag="IVK51_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP6431692.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12238..13449
                     /gene="thiI"
                     /locus_tag="IVK51_002125"
     CDS             12238..13449
                     /gene="thiI"
                     /locus_tag="IVK51_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP6431693.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4724 a         2275 c         2809 g         4003 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt aggggaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtgcg aatagttcaa attctcgaga tttgaataac tacttgaaca
     2761 aattactgcg taaagatgca gtagtgttac ggaaaatggt tgctcaagga gaaacattag
     2821 acgctattga agaaaagaaa gaagaaatgc tagaaagcat ttataacttc ttagctatct
     2881 cattaggaac acctccaaaa gaattcgatt ttgagtaccg cgatgaagaa aagaactatc
     2941 atcttgaccg aggactgaca ccacaaattt tttatgataa atacatcggt gtaaaattag
     3001 atgattatgt tagcgtgatc aatgcaccaa caaaagacaa accattcaat cgttcttata
     3061 cagtagagat gctaggaaat gtcgttggcg ggaaagaagt caaatatctg aatgttgata
     3121 tggaaacttt caaaaaatta gcgattgctc agcttgaaga aggagaatct gtttggtttg
     3181 gttgtgatgt aggtcaatct tctactagaa ctagtggcat catggcctta gatgcttatt
     3241 caatggatga tctatttgat acggatttta caatgactaa agctgaacgt ttagattttg
     3301 gagaaagtct gatgacccat gcgatggtat tgaccggtgt tgacttagta gacgggcaat
     3361 caacaaaatg gaaagtcgaa aatagctggg gcgaaaaagt tggaaagaat ggcttctttg
     3421 ttatgagtga tgcctggatg gacgaatata cgtaccaaat cgttgtaaga aaagatttat
     3481 tgacgaaaga gcaattaaat gcgttcaatg aagaaccaat tgttctatct ccgtgggatc
     3541 caatgggtgc tttagcatag acgagtattt caatgaagag tatgtagtaa agataaaaag
     3601 agctggctta agccagctct ttttatcttt aaagtccatt ccctgttttt tatggtccct
     3661 gtgctataat tctaaataaa ggataaggat tggaaataat ccttttatca ttaaaaatat
     3721 gatgacgaag tagaataaaa ttgggagtag gggggaaagt catggatatc caaactgttt
     3781 tatctgatct gaaattaaat ggtaaagtga ttccagttgt tggtggcgat gttaatcaga
     3841 cctatcgtat aaaaaccgaa catcgtgctt attttttaaa aatccatccg aatgtaaaaa
     3901 aaggattttt tgaagcagaa gtagacggat taaaagaact gtcagcattc gtacgtgtac
     3961 cggatacgta tatgctaggt gaaacaagtg aaggcgctta tttgttaatg gaatggatcg
     4021 agccgggaaa aggggatcaa cgagatcttg ctgcagcttt agctaatctc catcagcaaa
     4081 cagcgccaca atttggtttt cgtaaagata actatttggg tacacttgtt cagaaaaatt
     4141 cttttgaaga agattggtgg acttttttct ttaaagatcg gcttgaatcc cagatttctt
     4201 tggcagaaga aacgaatcga tggaatgtac agcgtcaaga aaagtatctg cgatttaaag
     4261 aacgtgtatt gaaatcggtt gaaccaaaaa agatcactcc acgcctcttg cacggagacc
     4321 tgtggagtgg taatgtgttc tttgaccagc aagggcatcc agtttttgtc gacccagctg
     4381 tttcttatgg gaatcgggaa caagatattg caatgagcca gttattcggt ggtttccgac
     4441 cggaattttt agatgcttat caaactatat ttccattaga aaagggatgg aaagaccgac
     4501 tgccgattta ccagttatat tatcttttgg cacatttgaa tatgtttgga gaaagttacg
     4561 gttcccaagt agatcaatta ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa
     4621 aatataccaa aaagcatgaa gtttcatatt atgagtgtga catcaaccaa acgatgacat
     4681 ttccttcgat gttaggagta gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa
     4741 gaggcagtga attcgtcaat agctttggac ttacttgggt catcacgaat tattccatgg
     4801 agattaatcg tctccctaga gtcggtgaag taatctcagt aacgactcaa gccatggagt
     4861 acaataagtt tttctgttat cggaacttct ggatccatga tgaagatggg aaagaattag
     4921 tcaaaataga atctgttttc gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta
     4981 atgaagagat cattgctcct tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa
     5041 aaattgaaaa gattgattcg ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg
     5101 atagtaatca gcatgttaat aatgcgatgt attttaattg gatcattgac gtattaggat
     5161 atgacttttt gactacgcat gttccagaaa aagtaattat tcgatttgat aaagaagtag
     5221 agtacgggaa tgagatcgaa agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa
     5281 caaaacatga aatccgaatg caagaacagc tgtattgtga agcaaatatt atatggaaaa
     5341 ataaataaaa aagaggttgt gacagaaggg ggcaacttca agaaataaga agaaatttac
     5401 gaaaattgtt tctcaaattt ttgtaaattt tcgacttagt tttcgaagaa gctatttttg
     5461 tcacagcctc tctttttaag tttagctatt agataatgat gtttagtttt ggataaacga
     5521 aatcaattct tcacccgaca aaccatagtt tacagcaatc aatagtttta aacgtgcttt
     5581 ttgactatta atactgttac agaaaataat ccccattttt tctagttctt ttcccccgcc
     5641 ttcataatca taaacaggct ctgcgattcc attgaaacaa cgagaaacaa gtactacagg
     5701 gattttttgg acaagaagct tttccaaagc agctaaggct tgtggaggga gattccccgc
     5761 acctaaagct tcgattacca aaccgtctac tttcgtatgg gcgatggctt ccaataagtc
     5821 cccttgcatc ccagcatatg ctttaacgat cgggatagtc ccatctacag cagaaatgtc
     5881 cagtcgcttt gtttctaaaa gttcctgcaa gaataaaatt cgattcttag ttactaagcc
     5941 gatgggaccc aatgtaggtg ttcggaaagt agcaacattt gtggtatggg tctttgtcac
     6001 gtagcgtgca gaatggatct catcgttcat gaccaccaaa acccccttat ctagcgcttc
     6061 ttcacaactt gcgacacgaa tcgcactttc aaaattataa aggccgtcac ttccaagttc
     6121 attacttgaa cgcattgccc cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc
     6181 aagaaaaaaa gcagtttctt ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat
     6241 acctgatgca taagcggaca agatacgttc ttttaatacc aacatgtgag aaggcatgat
     6301 gtgtggtgag ggaagattga agatatcttc aactaataat tctacatgct tggggattgt
     6361 gatttctgca tctaataatg ggtttgccac atcaggacta acaccgcccg tgagcatgtt
     6421 ttctttcatg gcgatcgttc cgccggtgtg tagtacaagt atttttttca ttttacagac
     6481 ttccttacga ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt
     6541 tgctatcagt gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa
     6601 atggaaaaga ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta
     6661 caagaaagca gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta
     6721 acatcgttta aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat
     6781 gcagggtttc tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc
     6841 tcagttgaga attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat
     6901 caaggaatca gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt
     6961 gcgcattttt tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta
     7021 gtccaattcc cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt
     7081 agaaagtggt ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc
     7141 gatgaatttc ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc
     7201 atcgatgagc cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg
     7261 aactttgctc tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa
     7321 gagtggcgta aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca
     7381 catgaagtca aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat
     7441 tctggtggtg atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca
     7501 gatggactca atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg
     7561 gcgaagtcta tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt
     7621 tcaaaagcgt tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta
     7681 tgcggtgtag caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa
     7741 ttagatatgt atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc
     7801 gaccaccctt ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat
     7861 cgacaaatcg tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga
     7921 caatcgattt ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg
     7981 atgaaaaaaa tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta
     8041 gagatattaa aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa
     8101 aagttagaac atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat
     8161 atcatcccaa aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa
     8221 attgaaatgg aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa
     8281 ggtgtaggaa ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac
     8341 tttgtgactc aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg
     8401 atttattagt caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa
     8461 agtagaaaca ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac
     8521 agaaaatgaa gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc
     8581 agctacttca cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt
     8641 agataaagca gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga
     8701 acaagtagat gcactgacag atatccttta ctttacttat ggttcttttt cattactagg
     8761 aatagtgcct gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc
     8821 agatggaaaa cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca
     8881 gatggaatat gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc
     8941 gattgctaag aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga
     9001 gtttcactat ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag
     9061 tgaatgcatg aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta
     9121 ttatttggat ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac
     9181 ggaggaacca taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt
     9241 cgccattgtc gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga
     9301 tgccttagaa aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga
     9361 tgttaaaaaa atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa
     9421 atggaatgac atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga
     9481 aaacctcaat gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc
     9541 tacaatggac caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg
     9601 ggaaacggaa gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact
     9661 gaaagaaaaa gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa
     9721 acagatcaaa gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga
     9781 ccctgttgaa gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc
     9841 tctgatgaaa aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact
     9901 tgaagaaatc agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga
     9961 agatcttgct gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct
    10021 tgaaaaaaca gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga
    10081 ttcgttatat gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca
    10141 atcaacgatt gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga
    10201 gctggatcac acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg
    10261 tttccaaacc gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg
    10321 tcaacacgaa attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt
    10381 agaagatatc gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg
    10441 cgaaaaagaa gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg
    10501 ttttgtagaa aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt
    10561 tgctactgac cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga
    10621 agaggtcaat cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac
    10681 acatgattta gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta
    10741 tcgtcataca cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa
    10801 agaatatcac tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc
    10861 tggtgctttc aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa
    10921 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
    10981 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
    11041 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
    11101 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
    11161 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
    11221 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
    11281 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
    11341 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
    11401 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
    11461 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
    11521 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
    11581 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
    11641 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
    11701 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
    11761 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
    11821 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
    11881 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
    11941 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
    12001 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
    12061 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
    12121 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
    12181 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
    12241 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
    12301 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
    12361 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
    12421 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
    12481 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
    12541 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
    12601 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
    12661 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
    12721 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
    12781 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
    12841 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
    12901 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
    12961 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
    13021 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
    13081 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
    13141 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
    13201 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
    13261 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
    13321 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
    13381 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
    13441 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
    13501 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
    13561 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
    13621 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
    13681 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
    13741 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
    13801 atcagaggtt g
//
LOCUS       DACCDF010000071        13743 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCDF010000071 DACCDF010000000
VERSION     DACCDF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.074"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            279..1064
                     /locus_tag="IVK51_002126"
     CDS             279..1064
                     /locus_tag="IVK51_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6431694.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1077..1871
                     /locus_tag="IVK51_002127"
     CDS             1077..1871
                     /locus_tag="IVK51_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP6431695.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKN"
     gene            complement(2051..2503)
                     /locus_tag="IVK51_002128"
     CDS             complement(2051..2503)
                     /locus_tag="IVK51_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6431696.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2968..3336)
                     /locus_tag="IVK51_002129"
     CDS             complement(2968..3336)
                     /locus_tag="IVK51_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP6431697.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3486..3968
                     /locus_tag="IVK51_002130"
     CDS             3486..3968
                     /locus_tag="IVK51_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP6431698.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4005..5015
                     /locus_tag="IVK51_002131"
     CDS             4005..5015
                     /locus_tag="IVK51_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP6431699.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5130..5843)
                     /locus_tag="IVK51_002132"
     CDS             complement(5130..5843)
                     /locus_tag="IVK51_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431700.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6033..6623)
                     /locus_tag="IVK51_002133"
     CDS             complement(6033..6623)
                     /locus_tag="IVK51_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP6431701.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7107..7334
                     /locus_tag="IVK51_002134"
     CDS             7107..7334
                     /locus_tag="IVK51_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP6431702.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7350..8516
                     /locus_tag="IVK51_002135"
     CDS             7350..8516
                     /locus_tag="IVK51_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP6431703.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8750..9793
                     /gene="hrcA"
                     /locus_tag="IVK51_002136"
     CDS             8750..9793
                     /gene="hrcA"
                     /locus_tag="IVK51_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP6431704.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9887..10450
                     /gene="grpE"
                     /locus_tag="IVK51_002137"
     CDS             9887..10450
                     /gene="grpE"
                     /locus_tag="IVK51_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP6431705.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10501..12330
                     /gene="dnaK"
                     /locus_tag="IVK51_002138"
     CDS             10501..12330
                     /gene="dnaK"
                     /locus_tag="IVK51_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP6431706.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVK51_002139"
     CDS             12481..13647
                     /gene="dnaJ"
                     /locus_tag="IVK51_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP6431707.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4611 a         2304 c         2888 g         3940 t
ORIGIN      
        1 aaaggggctg tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga
       61 agtagcttct tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc
      121 tttctttata gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca
      181 tttgctttag actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc
      241 gtaataacag gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca
      301 aagtcgcagt agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg
      361 ggaaagaaaa aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag
      421 cagtcaaagc aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt
      481 ctgaagaagg aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg
      541 tttgggttaa taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag
      601 attgggaaag agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt
      661 taaattactt tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg
      721 aacagatccc atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat
      781 ttaccgaaac ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc
      841 ctggtgctat ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac
      901 tcttggaaag tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag
      961 ctgcatggtt ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg
     1021 gtgggatgac tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg
     1081 gtttattgat tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa
     1141 agattggtgg cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg
     1201 gcttactcgt tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta
     1261 gtttactttc tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact
     1321 tgattggtgt ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt
     1381 tgattggtgt cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag
     1441 cagcaatcat tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa
     1501 aaaaaggcgg tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg
     1561 tatacagtgc attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa
     1621 tgatcggtgt ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt
     1681 ctgaagacaa gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg
     1741 cttatatttt ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa
     1801 acgtagttat ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag
     1861 aaaaaaatta acgattatcg gattattgct gatcgtagta ggaagcatgg tcacggtctt
     1921 tatttgagga taaagatggt aaaatttcat ttattaaaaa aatatttgtt tgaaccaaac
     1981 agtaagtact tataaaaaaa tagcgctaga atttccaaca tctctggaat ttctagcgct
     2041 ttttaattgt ttaagataca agggaaatat ctgtctggat caataatttt tcattgatcc
     2101 attgactaaa gtttacgaca taattgaagt taccaccgat tagtatctcc gcatgattgt
     2161 ctttataaag atcgtattgt aaaagacggt cttttttatt tgcgtaaaca tcgttatggt
     2221 actggactaa ttgttctcgg aattgattga ttgctgtaaa atcacgatat tgaaggttga
     2281 aacgcatcgt gattggtgca tttagcatga atcgctgcat ttcttttgct gcttcctctg
     2341 cttgatctcc gtataggaac tcaaaaagat tacgttcaga aaatttccat tgcccgccaa
     2401 cctttgttcc ttttagtttt ccttcatgaa gataatttct gatagaacgt gtagtaacac
     2461 ctaattgttc ggctaattca tttactgagt aaaaatcttt caatgttcac cctcctatgt
     2521 gaatggttct actgacttag ttgatatttg cttaatcacg aaatgtagaa agaacgattt
     2581 taattaaggt cattgtaaaa atcaagtcag aaaattctaa cttttatttc tctaaagaaa
     2641 ttcgttctct cctaaagtga tattatattt aaatatacct gaaaataatg ggcagtatta
     2701 tgaaagatca ctgatatttt ctcgtatttt ttccttattc aaaagttttg caggaaaatt
     2761 gctgaaaaaa taattttact aagtagaaaa ttcaaagcca ttcaatcagt gatttttttc
     2821 agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt gtttaaccag
     2881 ggatgataaa aaggatagga ttgaacaaaa cctgtataag gtgatgaata gtttcctcgc
     2941 cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac cttttttatc
     3001 ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac ttttccaagg
     3061 aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt tttcaagggc
     3121 acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac taaatgcaat
     3181 aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg tgtctagctg
     3241 gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat gttctttttc
     3301 tttgatttga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc attaagtggt
     3361 ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa acacaagtaa
     3421 tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag aaaagagaca
     3481 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
     3541 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
     3601 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
     3661 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
     3721 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
     3781 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
     3841 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
     3901 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
     3961 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
     4021 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
     4081 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
     4141 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
     4201 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
     4261 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
     4321 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
     4381 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
     4441 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     4501 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     4561 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     4621 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     4681 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     4741 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     4801 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     4861 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     4921 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     4981 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     5041 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     5101 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     5161 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     5221 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     5281 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     5341 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     5401 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     5461 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     5521 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     5581 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     5641 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     5701 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     5761 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     5821 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     5881 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     5941 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     6001 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     6061 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     6121 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     6181 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     6241 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     6301 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     6361 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     6421 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     6481 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     6541 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     6601 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     6661 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     6721 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     6781 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     6841 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     6901 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     6961 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     7021 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     7081 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     7141 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     7201 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     7261 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     7321 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     7381 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     7441 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     7501 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     7561 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     7621 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     7681 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     7741 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     7801 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     7861 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     7921 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     7981 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     8041 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     8101 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgaccac tattttgggt
     8161 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     8221 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     8281 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     8341 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     8401 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     8461 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     8521 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     8581 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     8641 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     8701 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     8761 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     8821 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     8881 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     8941 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     9001 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     9061 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     9121 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     9181 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     9241 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     9301 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     9361 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     9421 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     9481 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     9541 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     9601 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     9661 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     9721 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     9781 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     9841 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     9901 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     9961 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
    10021 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
    10081 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
    10141 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
    10201 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
    10261 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
    10321 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
    10381 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
    10441 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
    10501 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
    10561 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
    10621 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
    10681 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
    10741 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
    10801 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
    10861 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
    10921 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
    10981 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
    11041 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
    11101 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
    11161 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
    11221 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
    11281 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
    11341 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
    11401 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
    11461 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
    11521 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
    11581 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
    11641 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
    11701 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
    11761 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
    11821 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
    11881 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
    11941 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
    12001 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
    12061 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
    12121 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
    12181 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
    12241 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
    12301 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
    12361 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
    12421 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
    12481 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
    12541 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
    12601 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
    12661 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
    12721 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
    12781 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
    12841 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
    12901 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
    12961 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
    13021 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
    13081 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
    13141 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
    13201 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
    13261 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
    13321 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
    13381 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
    13441 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    13501 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    13561 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    13621 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    13681 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    13741 caa
//
LOCUS       DACCDF010000072        13536 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCDF010000072 DACCDF010000000
VERSION     DACCDF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.075"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(60..1007)
                     /locus_tag="IVK51_002140"
     CDS             complement(60..1007)
                     /locus_tag="IVK51_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP6431708.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1197..1466
                     /gene="rpsN"
                     /locus_tag="IVK51_002141"
     CDS             1197..1466
                     /gene="rpsN"
                     /locus_tag="IVK51_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP6431709.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1642..2004)
                     /locus_tag="IVK51_002142"
     CDS             complement(1642..2004)
                     /locus_tag="IVK51_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6431710.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2015..3136)
                     /locus_tag="IVK51_002143"
     CDS             complement(2015..3136)
                     /locus_tag="IVK51_002143"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP6431711.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYIHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3152..3502)
                     /locus_tag="IVK51_002144"
     CDS             complement(3152..3502)
                     /locus_tag="IVK51_002144"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP6431712.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGKVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3632..5143)
                     /locus_tag="IVK51_002145"
     CDS             complement(3632..5143)
                     /locus_tag="IVK51_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP6431713.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5465..6817)
                     /locus_tag="IVK51_002146"
     CDS             complement(5465..6817)
                     /locus_tag="IVK51_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP6431714.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAVKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6970..8046)
                     /locus_tag="IVK51_002147"
     CDS             complement(6970..8046)
                     /locus_tag="IVK51_002147"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP6431715.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8242..9987
                     /locus_tag="IVK51_002148"
     CDS             8242..9987
                     /locus_tag="IVK51_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP6431716.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLKKEEQTQASRKTEIVHEKEFGSFAFRASTSTEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10236..10703)
                     /locus_tag="IVK51_002149"
     CDS             complement(10236..10703)
                     /locus_tag="IVK51_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP6431717.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFCQALKKAIDQGLPSTEDSR"
     gene            complement(10687..12060)
                     /locus_tag="IVK51_002150"
     CDS             complement(10687..12060)
                     /locus_tag="IVK51_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431718.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGIMYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12381..12869)
                     /locus_tag="IVK51_002151"
     CDS             complement(12381..12869)
                     /locus_tag="IVK51_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431719.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12891..13493)
                     /locus_tag="IVK51_002152"
     CDS             complement(12891..13493)
                     /locus_tag="IVK51_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6431720.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3863 a         2938 c         2458 g         4277 t
ORIGIN      
        1 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
       61 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
      121 gcttggatga catgcttaag ggaagcagac agattcattg aaataaaaac atgaaaggct
      181 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
      241 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
      301 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
      361 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
      421 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
      481 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
      541 ccgaccgcac ccgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
      601 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
      661 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
      721 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
      781 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
      841 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
      901 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
      961 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
     1021 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
     1081 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
     1141 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
     1201 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
     1261 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgcaaa cttcctttag
     1321 atgctcgtcc agaacgattg aaaaatcgtg atttgatcga tggacgccct cgcggataca
     1381 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
     1441 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
     1501 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
     1561 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
     1621 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
     1681 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
     1741 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
     1801 tattcctgtt ccagtagctt tgatcccaat atgagtcgga agcttaggtt tagccatttt
     1861 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
     1921 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
     1981 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
     2041 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
     2101 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
     2161 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
     2221 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
     2281 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
     2341 ttccttcacc tgctgggagt tttttgactt gaattaattc agaaaccagc tcaagtgctg
     2401 gctgcagtgg ataaacttcc ggtagtgcgt gcccagaggg attcagtccg tacattgcta
     2461 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
     2521 tgacatgtat atatcgagga agttcgggaa gtttttttag cacttctata aagcgttctt
     2581 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
     2641 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
     2701 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
     2761 tctctagctt acttttctct aaaacagcag ctgctgcctg caaccattcc cgtgtacctg
     2821 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
     2881 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
     2941 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
     3001 gaccgtatcc gtttgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
     3061 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
     3121 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
     3181 ctgtttcatt tgtatgagta atactgaccc atactttgcc ttcatgaggt gaacgcgtca
     3241 caacaggctg accgttttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
     3301 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
     3361 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
     3421 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
     3481 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
     3541 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
     3601 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
     3661 cgcgtttttt atcgttgtga cgtttaccgc catctttttg gctgttatta tttttgctat
     3721 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
     3781 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
     3841 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
     3901 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtacttat
     3961 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
     4021 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
     4081 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
     4141 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
     4201 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
     4261 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
     4321 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
     4381 attcgtctac acggcgtttt gtacgtccga agacgattgt tagttcaggt gtttgaacat
     4441 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
     4501 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
     4561 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
     4621 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
     4681 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
     4741 tgatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
     4801 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
     4861 cgcgccctaa acgatataat tcttcttgtg tttggatagc taactcacgt gttggtgcga
     4921 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
     4981 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
     5041 ctagtggaat cgtttctgat tggataggtg ttgcttcttc aaaaccagcg cgttcgactg
     5101 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
     5161 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
     5221 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
     5281 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
     5341 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
     5401 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
     5461 atttttattt catttgctga agctgttcta ccacttcaaa taagcccatg ctgttgctgc
     5521 cttttagcat aatactatct gttgcttcaa gattttgttt tacagcatca atcaatttat
     5581 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
     5641 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
     5701 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
     5761 acacagcgat tctgcgtcct tttgttggta gatttttaaa agtatctaat accagtccca
     5821 tggctgttgg atttgcatta tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
     5881 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
     5941 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
     6001 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
     6061 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
     6121 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
     6181 cgtctggtac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
     6241 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
     6301 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaag acgatctttt
     6361 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
     6421 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
     6481 tggttttacc attgcttcct gtaattgcaa cgacttctgc acctactttt tccaagtaat
     6541 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatctt
     6601 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
     6661 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
     6721 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
     6781 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
     6841 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
     6901 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcttcttctt
     6961 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
     7021 taattgaatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
     7081 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
     7141 gtttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
     7201 agctaacttc gcgtactctt gcgctttttg ataaacttct tccggcactt cggctggaat
     7261 ctgcatttcg attttattat tgatatattt ggcttcataa tcatagaatg ctacgtcttt
     7321 tacgacttcg ccaggcaaag tcgtccgaac atcttcattt cctaatacag caacttcgat
     7381 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
     7441 taaagcattt tgcagctctt ctcggttttc tgcctttgta atgccgacac tagaacccat
     7501 attcgcaggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
     7561 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
     7621 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
     7681 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
     7741 cccgtttggc ccgtgtaaaa ctggaaaaac gatggctcct tcttctttga tttctcccgg
     7801 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
     7861 caagacatct ttgctagcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
     7921 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
     7981 aaatgcggaa agaatggaaa cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
     8041 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
     8101 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagttggttt ctaaaagaac
     8161 aaacgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
     8221 taaagaaaag gagggatgat gatggcgcaa acaaatccac gtagccgctt ggcagaaatc
     8281 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
     8341 caggtaaaaa aagcctttga agaactaggc cccaccttca ttaagatagg acagatgctt
     8401 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
     8461 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
     8521 tcattatcag atatattcga tgatttttcc aaatcgcctt ttgcctctgc ttctatgggg
     8581 caggctcatc gcgccaagct gaagaacggt gactcggtcg tcgttaagat ccaacaccct
     8641 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
     8701 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
     8761 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
     8821 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
     8881 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga accatttaat gaacgccgaa
     8941 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
     9001 aagcttttag ttgagaactt tatgaaacag gtatttgatg atggtttttt ccatgctgat
     9061 cctcatccag ggaatctgct attccatgta ctaaaaaaag aagaacagac acaagctagc
     9121 cgaaaaactg aaatagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
     9181 tctacagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
     9241 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
     9301 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
     9361 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
     9421 gatgaaatca acttgcaaga agtattcaca cacgtcgtga cgatttgtca tcaaaataat
     9481 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
     9541 atccgcgtcc ttgaccctga agtttccctc atggaagtcg cttcgccttt tgcccaacat
     9601 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
     9661 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
     9721 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
     9781 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
     9841 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
     9901 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
     9961 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    10021 aaataagaag tgattttgca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    10081 ttcgaagagg ctacttctga agcaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    10141 cagtaataag aacaatcaaa aaggaacatg gagcacattt atcctgtttc cctgttccct
    10201 ttttgatgta ttcgagcata aataaaatac cttcgttacc tactatcttc tgtacttggt
    10261 aaaccttggt caattgcctt ttttaacgct tgacaaaaag acatatgatc tttctccgta
    10321 aagggttttg gaccctttgt ccttatgcca gcttttctta actttccact atgataacgt
    10381 tgttctagaa gcgtgccgat attcgcttcg tcaaattctt ttccagagtg atggaacact
    10441 cggacgccct tcggcaatac aagtgaagct aaaccataat cttgcgtgat caaaaagtca
    10501 ttcttcttaa ccataccgac gattttataa tccgcactat cagctccgcg gtctacgtag
    10561 acgatttgga catgattagc gtattctttg gtcgtataat ggtccacact ggtcacgata
    10621 accacgtcaa gaccatagga ttctcctaat tttatgacat cttcttttac tggagaacca
    10681 tctccatcaa tgaatagcct cattcttttt cgccttcttc cataaatgat aaacagttac
    10741 taatccgctc agtaaaagac caacattgat ccattttgcc aagataagca gttgtacact
    10801 ttctatcggc actgattcat ggttcagcgt gatcaataat agatggattg gctctcccat
    10861 ttcttttgct aaaacaaaca aaagcgtcat caataaaaac tggtggatca cactcccttt
    10921 attttgagta gtatacttga caacgatccg gcgatccatt ggaatagctg catcagtttg
    10981 ataatattcc ttatttagcc agctgcctgt tactacgtgc cagaaactaa gcagaaagac
    11041 acctatcgca aaaccatttg gaatcaagag gaccaataaa gaagaactca gtccaagcaa
    11101 ttgcatagcc aatggagacc atgttaaacg tttcaaaatc catggtccga ctatttttgc
    11161 tgcaatcaag cctgctgcat aaggcaaata cataaaaatc gaaagttttt ctaatccgta
    11221 cattatgcca acataaaaac tgctaaataa aaaaaggaaa ttccctagca ttccatttag
    11281 aaaagtaaac agatttagcc agcttggcaa cttccattcc ttcttctggt gtcctaaaac
    11341 aaacacgaac aagatgaaca gcagcaagaa accataaata gcaagatcaa aaagcctttc
    11401 atcgaataac aatcgggcat ttctcaaaag aaacaataag ccaaagaata tcaaaaataa
    11461 aacaagctct cgcgtagcta ccagatttct tttgatgtct ttgaaatcca gttcgtaacg
    11521 cggataatga ctaaccgtat ggtaggccat gacgtaaaaa agtgtataaa tagtaaagct
    11581 tgctgcaatc tgagtcggtg caggcaactc aatcgcttga tacaggagaa atagccagat
    11641 agctgcaaat aagtactttt tcccatccat attgataaaa ccttgcgtct tttcgtgaaa
    11701 attgaccgtg gtattagcag gtacaagcca tgctgcactc agccctagca aaacacttga
    11761 aaaataaaat aaaggaaaat aaaatacgcc taatattcca agcaatgaac cagcaccacc
    11821 aatcagcaga gcgatcatca gcaacgtgta actatctaga ccaaatcgta gactgttgac
    11881 taaaaatagt ccagtcatcc gaaaggcata aaacagcgta aatggcaaaa tcgtcagcca
    11941 attatgcgag tctgttagtc ccaaaaataa aagataagga ataaaaataa ccgtgttact
    12001 taagataaag ggcgttgttt gtgtaaaatt acgcccaaat cgtttgagtt tttcgaacat
    12061 gacaatggct ccttaaacag tcttataact aatctgctct tcgattttag cattaaaggg
    12121 gaaataaacc aattttacag aatatattct tgtaaaaaaa atacagaaag aagcataagc
    12181 ttctttctgc atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag
    12241 tcttgcccga cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc
    12301 tccaaaatca attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta
    12361 ctttaacaca gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga
    12421 aatccttgac cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa
    12481 acctcgattg cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt
    12541 tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact
    12601 ttatttagca aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg
    12661 taaccgacag tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct
    12721 ttttgaatct gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca
    12781 atgatcggca caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt
    12841 tcattttctg ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga
    12901 ccttgcgctt gatggaataa aaagagcatg atcatgctat acacacccac cgaccacggt
    12961 ttcaagaaaa tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact
    13021 agacagatca agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta
    13081 atatctttcg tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa
    13141 ataagctgga caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca
    13201 atcactaggc caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc
    13261 gctactgagg agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga
    13321 acaaatatca tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc
    13381 gatcgaaaaa tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca
    13441 ataatgggca tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt
    13501 ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DACCDF010000073        13346 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCDF010000073 DACCDF010000000
VERSION     DACCDF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.076"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..1364)
                     /locus_tag="IVK51_002153"
     CDS             complement(45..1364)
                     /locus_tag="IVK51_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6431721.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IVK51_002154"
     CDS             complement(1361..2014)
                     /locus_tag="IVK51_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6431722.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IVK51_002155"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IVK51_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IVK51_002156"
     CDS             complement(2724..3440)
                     /locus_tag="IVK51_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP6431723.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IVK51_002157"
     CDS             complement(3564..3749)
                     /locus_tag="IVK51_002157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431724.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IVK51_002158"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IVK51_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IVK51_002159"
     CDS             complement(5356..6387)
                     /locus_tag="IVK51_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431725.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IVK51_002160"
     CDS             complement(6394..7224)
                     /locus_tag="IVK51_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431726.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IVK51_002161"
     CDS             complement(7221..8027)
                     /locus_tag="IVK51_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6431727.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IVK51_002162"
     CDS             complement(8033..8857)
                     /locus_tag="IVK51_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP6431728.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IVK51_002163"
     CDS             complement(8847..9947)
                     /locus_tag="IVK51_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP6431729.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCVSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IVK51_002164"
     CDS             10125..10910
                     /locus_tag="IVK51_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP6431730.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IVK51_002165"
     CDS             10943..11326
                     /locus_tag="IVK51_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP6431731.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IVK51_002166"
     CDS             11402..11533
                     /locus_tag="IVK51_002166"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6431732.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IVK51_002167"
     CDS             11502..11738
                     /locus_tag="IVK51_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6431733.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IVK51_002168"
     CDS             complement(11816..12787)
                     /locus_tag="IVK51_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP6431734.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2911 c         2306 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagacgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DACCDF010000074        13334 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCDF010000074 DACCDF010000000
VERSION     DACCDF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.077"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="IVK51_002169"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="IVK51_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP6431735.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /locus_tag="IVK51_002170"
     CDS             complement(740..1462)
                     /locus_tag="IVK51_002170"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP6431736.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /locus_tag="IVK51_002171"
     CDS             complement(1598..2479)
                     /locus_tag="IVK51_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP6431737.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IVK51_002172"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="IVK51_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP6431738.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="IVK51_002173"
     CDS             3719..4198
                     /locus_tag="IVK51_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP6431739.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="IVK51_002174"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="IVK51_002174"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP6431740.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="IVK51_002175"
     CDS             5499..5627
                     /locus_tag="IVK51_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6431741.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5631..6176
                     /locus_tag="IVK51_002176"
     CDS             5631..6176
                     /locus_tag="IVK51_002176"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP6431742.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6629..8320)
                     /locus_tag="IVK51_002177"
     CDS             complement(6629..8320)
                     /locus_tag="IVK51_002177"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP6431743.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IVK51_002178"
     CDS             complement(8740..9687)
                     /gene="arcC"
                     /locus_tag="IVK51_002178"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP6431744.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IVK51_002179"
     CDS             complement(9802..10821)
                     /gene="argF"
                     /locus_tag="IVK51_002179"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP6431745.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IVK51_002180"
     CDS             complement(10912..12141)
                     /gene="arcA"
                     /locus_tag="IVK51_002180"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP6431746.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12602..13303
                     /locus_tag="IVK51_002181"
     CDS             12602..13303
                     /locus_tag="IVK51_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6431747.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         3752 a         2712 c         2367 g         4503 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttcaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaaaaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacacaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaacc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacaa gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
     6481 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
     6541 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
     6601 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
     6661 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
     6721 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
     6781 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
     6841 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
     6901 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
     6961 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
     7021 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
     7081 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
     7141 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
     7201 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
     7261 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
     7321 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
     7381 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
     7441 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
     7501 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
     7561 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
     7621 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
     7681 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
     7741 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
     7801 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
     7861 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
     7921 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
     7981 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
     8041 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
     8101 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
     8161 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
     8221 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
     8281 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
     8341 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
     8401 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
     8461 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
     8521 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
     8581 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
     8641 ctagtcgttt cataaaaaaa aaatccagat ggcctaaagg agaagaaata ccacctggat
     8701 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
     8761 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
     8821 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
     8881 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
     8941 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
     9001 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
     9061 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
     9121 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
     9181 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
     9241 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
     9301 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
     9361 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
     9421 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
     9481 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
     9541 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
     9601 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
     9661 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
     9721 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
     9781 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
     9841 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
     9901 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
     9961 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    10021 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    10081 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    10141 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    10201 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    10261 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    10321 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    10381 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    10441 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    10501 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    10561 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    10621 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    10681 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    10741 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    10801 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    10861 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    10921 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    10981 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    11041 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    11101 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    11161 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    11221 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    11281 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    11341 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    11401 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    11461 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    11521 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    11581 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    11641 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    11701 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    11761 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    11821 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    11881 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    11941 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    12001 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    12061 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    12121 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    12181 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    12241 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    12301 ttttattctg tatacacttt caaaaaaata ttaattcatt gaaaaaattt ttttgaataa
    12361 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    12421 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    12481 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    12541 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    12601 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    12661 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    12721 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    12781 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    12841 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    12901 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    12961 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    13021 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    13081 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    13141 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    13201 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    13261 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    13321 agtgaccctg ataa
//
LOCUS       DACCDF010000075        13331 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCDF010000075 DACCDF010000000
VERSION     DACCDF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.078"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IVK51_002182"
     CDS             complement(365..1156)
                     /gene="msrA"
                     /locus_tag="IVK51_002182"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6431748.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
     gene            complement(1347..1982)
                     /locus_tag="IVK51_002183"
     CDS             complement(1347..1982)
                     /locus_tag="IVK51_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6431749.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(2248..3300)
                     /locus_tag="IVK51_002184"
     CDS             complement(2248..3300)
                     /locus_tag="IVK51_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6431750.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(3313..4251)
                     /locus_tag="IVK51_002185"
     CDS             complement(3313..4251)
                     /locus_tag="IVK51_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6431751.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(4702..5352)
                     /locus_tag="IVK51_002186"
     CDS             complement(4702..5352)
                     /locus_tag="IVK51_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431752.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(5452..5961)
                     /locus_tag="IVK51_002187"
     CDS             complement(5452..5961)
                     /locus_tag="IVK51_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP6431753.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(6324..6707)
                     /locus_tag="IVK51_002188"
     CDS             complement(6324..6707)
                     /locus_tag="IVK51_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6431754.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            complement(6948..7562)
                     /locus_tag="IVK51_002189"
     CDS             complement(6948..7562)
                     /locus_tag="IVK51_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431755.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            7706..8267
                     /locus_tag="IVK51_002190"
                     /pseudo
     CDS             7706..8267
                     /locus_tag="IVK51_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(8378..9352)
                     /gene="lacD"
                     /locus_tag="IVK51_002191"
     CDS             complement(8378..9352)
                     /gene="lacD"
                     /locus_tag="IVK51_002191"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6431756.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(9371..9826)
                     /locus_tag="IVK51_002192"
     CDS             complement(9371..9826)
                     /locus_tag="IVK51_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6431757.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            complement(9854..11299)
                     /locus_tag="IVK51_002193"
     CDS             complement(9854..11299)
                     /locus_tag="IVK51_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431758.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            complement(11296..12231)
                     /gene="pfkB"
                     /locus_tag="IVK51_002194"
     CDS             complement(11296..12231)
                     /gene="pfkB"
                     /locus_tag="IVK51_002194"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP6431759.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            12399..13157
                     /locus_tag="IVK51_002195"
     CDS             12399..13157
                     /locus_tag="IVK51_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6431760.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
BASE COUNT         4156 a         2794 c         2067 g         4314 t
ORIGIN      
        1 tagttattgt ttgcgctttt atacggaatt tagttcatca ttaatgtgga attcgattag
       61 gtgtctatta attatcaaga tgattctcta caaaattcca tagaaaaatc aagattctac
      121 aattcgaatt ctctttcaca tggaaataaa ggctagtaaa aaaacaggag ttgtgacaaa
      181 agacttgtca tacctcctgt ttccttgcga cttgaagctg aatgctttgc gccacaacct
      241 tttcctcggt gttctcaaag caactcctcg aaataattca taactgtcca aaaatatgaa
      301 aagctatttt tgtcagttgc tcattatttt gtcggagctg aacgctttgc gccacaacct
      361 tcctctatct atgtcgctga aaaaataaat gaagctgatg cgaatgcttg atctttttat
      421 atcttttagg ttgcttctga taaaattgtt gatgatgatt ttcagctggc caaaaagtct
      481 ttgcttggcg aatctctgta acgataggac ggctaaatcg gttagattga atcagttgtt
      541 ctcttgattt ttcagccaat tgcctttgct tttcattaac aacaaataga attgaacgat
      601 attgtttccc ccgatcttga aattgaccaa aggcatctgt agggtctgaa atttgccaat
      661 aaatctccaa cagctcttca taggttattt gacgtgtatc aaatatgatc tcaacagctt
      721 caacatgccc agtatatcca ccactgactt gatcgtaatt tggttttttg gtctctccac
      781 ctgtataacc agataacact gagattacac ctggttttgt ttcaaaaggc tcaaccatac
      841 accagaaaca gccaccggca aaaatcgccc gttcttggta taagggtgaa gttatcttat
      901 gtttggcata atcataagcc tttgcactcg ctgactcccc tgtgattctt aaataaaaat
      961 ccatcgcatc tggtgtcaaa ttatctcgta gtgctagcgg acgcaagttg acctctagtt
     1021 tttctatcac ctgctttatc gacatttttg ctaggtcttc ttttgcctct tcaaattgtg
     1081 ttctttccca ttctcttgta tttcgattta aaataatatt ataaaggtct atttccatct
     1141 gctccacatc taacatgaag tcaccctctt tcaagttttc ttcttattca ttcaaaaaat
     1201 gactacctga aatttagcac aacgcataac aagttaagac taacttgctt ttcgctctcc
     1261 tcatctctat tcttagtcga acacatccac acgaatggtt acccatagag aaattctttt
     1321 acttaatcag cttcacactg ctttttctac tttcgtagtt gaaaaaaact taaacaaatc
     1381 atcggcaaat agttaaagaa aaaacttaag cgttgaaaga agcccaattg cttaaagaag
     1441 gcaaactgac taatcgacgg aatccgtgct aatccgtaaa gacctgcaat tagtaaacta
     1501 ataactgtta aaacaagata aaaatggcta agcgtacctt gaccactttg acgataaagc
     1561 agaacaagta acaatgggaa aagtagaaag cctgcatagc ctagcccaga acctatatta
     1621 tggatccaag tcgatagatt ccaagagctt tcattcgtat tgatactgaa taacccagta
     1681 aaaatacaat cacctatccc atataaacca actgttgcaa cagcaagtat tgcaagtgtt
     1741 tttgatgttc caataaatag gtgatacaac gcaggcaacg aaagaacaaa aaaaagtcca
     1801 gatacaactg accagaccaa gaaagcacgt ctgactggac tatctacgtc accaaacaca
     1861 ctaattacgg tagtcatttg attcagtttg ggataaaaaa ggccaaggat atacggtgtc
     1921 gataaatcac taatcacacc taataataaa aaataaaaac catatttttg caaaaaaatc
     1981 attttcttct cctacttttt agtgattatt cgatacatcc aacaatcaaa ttttttctca
     2041 ttttaacaca tatttttatt ctagttgcct tttgaacaat caataacata aaggtgagtt
     2101 tcttctacat tttaaaaaat cgaatggcac tgaagagttc agttgtcagc atcaatctcc
     2161 tactcaaaaa ggcaaaaaaa cgtaagtcag atgttgttga catctcactt acgcatccat
     2221 tgattgaaaa tttcttagta aaatctacta gcctaaaaat ttgaagactt cttctcgatc
     2281 atacagttta aatactttct cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat
     2341 gactaaccaa caaagaacaa caagaaagcc atttagccct gcatctaaag aagccaacgg
     2401 accaacaagt ccgaccacgc cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag
     2461 agcgacagga atagctgtaa tcactgcact aactaaacaa ggaataagaa tcacaggttt
     2521 tctaaataga ttaggcatca tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc
     2581 tgacttatta accttccaag agttcactac taaaacaaaa gtggttgcag ctacgcccat
     2641 cgcagccgca ccagcagaaa caccatctag ttggattgcc attccgattg ctactgttga
     2701 aataggagag ataatcaaaa aggcaaagga acaaccaatc aagatagaca ttaacagcgg
     2761 ttgtaaggta gtgaagttat taatcaatga tccaattgca gaagtaattg ctgttacaaa
     2821 agggaataaa taaaaaccaa gtaaacctgc acctacacct acaacaatcg gtgttaacac
     2881 aatcgacact gacccaaaac tatttccaat cagtaaaatc agcccgacag caatcgatgc
     2941 tgtcagcatc gtattaataa tatcgcctgt accagcacca acgtaaacac ccgtttcacc
     3001 caaatcaggt gtgaatttga ctacaccaga accaacaaaa gaagctccgg ctacaaccat
     3061 cattctcatc ggatcaaatc caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt
     3121 tgctagttga aaaataactg ctaactgtgc gatcgttacc gcaaagggat acgcactaaa
     3181 atattttaaa atggcaccta atactgcatt aggaatcaat ccaataatga ttcctgttgc
     3241 tgtaccagcg aggactttat tcagaaaaat tttcggtgat attttttgtg aagtttccat
     3301 tcaatctctc ctttatttta cgttcaaact atcttctttt gcatggacaa gctcagtcaa
     3361 gaaatcacaa taaggcgtcg caacaccata tttcttccct tttctagaaa ttgcgccatt
     3421 gatataatcg atttctgtta atcgatggtt cttaatcaag tcttgataca ttgaaggata
     3481 atgtaaccca attgtttccg ggtcaaaaca agattcacaa tgtgcaatga cttctgggac
     3541 atctagttca atcttctcta ctgctgctac tttggcaaac tcattgacaa tcgtcgccac
     3601 cattttatga gcagtcgatg tttttcctag ctcagccata ttaacgtcta agattgtgca
     3661 tagtccgttc attgttccgt tcacacatgc tttgcgataa atagaatagt gaatattgtc
     3721 agaaaaatga gcatttaatc ctgattcaga caatttgtct gctaattttt ttgcagctgc
     3781 ttcttttcca tcacctaaat tttgtaattc tactgaacca cttccaaata atttgacctg
     3841 accaggacct tcaagacctg ccgtccacat ggtatttcca atatagatat tttccattgg
     3901 tacaaatttc tcaataatat cttcatgccc gataccattt aatagacata agacctctgt
     3961 atcttttttg attaatgatt ggatatcctg cagcattttt tccagctgca tcgctttggt
     4021 aaatagaata atcagatcaa cttgatcttc tttttctact tcggattgaa gaacgattgg
     4081 tagttttgct tctacctctt taccattaaa atttgcttgt aatccatgct ctttgatttg
     4141 ttggacatgt tctgcccagc catcgattaa taatacttcg ttcccacttt ggtgaagcat
     4201 taacccaaag cgacttccca ttgcgccagc gcctgcaatt gctattttca ttgattcttc
     4261 ctcctttttc tttctattat attcatttga gaatcatttc acaaccttgt tttgttattc
     4321 ttctcttatt tatcatgttt atatccacca ttcaagttat tttatcagca aatcaataaa
     4381 aactggaata gaatcccaat ttcatcgtaa gagaaactag actagcaata agaaaataga
     4441 aacattcata gaaaaaacct cactcctctg attataaaag agggggcatt aacctcgtgt
     4501 atactaaaac cttccattca ttcgatggat gattttcttt ttgtgaaaaa atattcattg
     4561 tagtacgatg ttgcgcaaca cagacggatg gtattgctta ttatttatta aaatcgaaca
     4621 atacacttat ttaaaaactt ctttcccaga ttttcttcca tttttacagt tataactagt
     4681 attatcatga aaaatagcct tttattgtct atgggaataa taatttaaaa ttggtacttt
     4741 tttattaatt agttcatcgc ccaataatct ctccagtttc ttagcatctc ctgactcaac
     4801 cggaattatc caagcccagt ccaaataatt ttttaaaatc tcatcatatt ttgcaaatat
     4861 ttcattgcca tattccgtcc ttaaaattat attttcagtg ccagacaagt gatttgtatt
     4921 gagttgataa aaaagatctc catctaaaaa aatatcatta gaagaagatt tccccacagt
     4981 aatgaccatt ccatcagctc tatgtgttac atacaaataa ttgtatgacc tcatttcacc
     5041 taatgaatca attatttctt gccctttagc tctaatatat tgatctctat tacctatctc
     5101 actcttattt aacaaaagtt ttttcatgtc aaatttaatc ggctgtttta tttttgtaaa
     5161 ttcgcttcta ctactaatcc aatccgagat taatttttca acatgtccca gagtaaactc
     5221 tgatttattc gctaaatact ttttttgttg tattaaagac atctttcccc gaagctcaaa
     5281 attgagccta catcgattct ctatctcttc aaaaagcatc ttatcttcat cattcttata
     5341 ttcatcattc ataaaaccaa tccagctcct cgattaagat aatattttaa tctcatacaa
     5401 aaactagctc tcatcaaaac aaccattgtt ttgatttctc atgaaatttc tttatacttt
     5461 tctccgatta taaattccta accccttgag aaaattacgc atatatttat ctccgcaagg
     5521 cttaaaattt cgttggcctt cttttctcaa aaatgccccg atttcgcctt ttgatggata
     5581 gagttctgcc tcagcaaaaa cttctaaaat atcatcagtg gtcatagaca aggcgatttt
     5641 taattttttt agcaacacat tgttgatata tttgatttcc ttgctttttt gcggcgtgtc
     5701 tactatcggc ttatcttttt gcggaccacg cgccaagaga atcaggccat ttaggaatgc
     5761 gtcaaaaact tggttgttga tggttttttc atacacatta ttttctgcat tatctttgtt
     5821 cttatcctgt actttggtca gtaccgcctg agcttcttct ttagtcagtg gtacaccgcc
     5881 tagctcaaaa gctttaatca aatccgtatc tttcaaatca atcgcataac gcaatcgcaa
     5941 taacctatca ttgttattca tttgttcctc cgaagtattt agtctttact ttcttacgta
     6001 ctttttgtct tgctaagatt tttaggatca atgataaaaa ccgcatcact tatgatacgg
     6061 agctgggtat catgagtgaa tgatatttat aaatcactgg atattgaagt atattctgtt
     6121 ttctcaactt atttagtcag attttttaat gaagattttt tgagtaaaat ttctataatt
     6181 cctacttatt gaaaagtagt ttgtagtgct gcttctacct ctctcatctg ttctacaaat
     6241 tccaaataac ttgacgcagc ttgcggaatg aaaaaatgat atgtgaatat ttctcctcct
     6301 agcccgctta atatgcctat tttttatacc catgatttgc aggaagggga tcggaagtag
     6361 gttgcagaaa gccgcctttc ataatctgtc ggaaaccgga catcaaaacg gattatttta
     6421 cttgtcacat ttgtattttc ttgtgaggaa gtgccaaata cattatttac ttgagtgaca
     6481 ttctcattgt cctgtctgat attgcttcgt ataaaagtac cagatccacg tctcgtatat
     6541 aacaaacctt catcaataag gagttgtata gctttgcgga tagtaacacg gcttgtttta
     6601 aactctctag ccagactttc ttgctcaggt aatttagaac cttcctgata ttttccctca
     6661 acgatcctct cttttagagc caaggctatt tctttatatt ttggcatatt ttcccctcat
     6721 ttcattgatc ctacaaaaaa attagtatat atacaaattt ttgtaaagta tatatactaa
     6781 taaatacaaa aatattttta ttgttggata cagaaatcta tttttaactg gggatatctt
     6841 tgccagctaa tatctttgag actacaccta tcttcatcta aatggatcaa ttactcgctt
     6901 tttccattga ccttcgtggt acttctgctt tctgatagta aaaaacatta ctgaatgatc
     6961 ttactatata acatacagcg gagatctact tctttattaa ggatcttcat gacgttatct
     7021 ttttcttttt tccgttcgaa accgcgtcgt tcataaaatt gatacgtaca gccgctatcc
     7081 gtgaacagaa atacttcttt tccttgctct cggcgttcaa attcttttaa taatttacta
     7141 ccgatcccct tgatctttaa atcagggttg gcagctaaaa agacaatttc tccatctggg
     7201 gaatgttttt ttaaatagtc tgaaagcaat tcgtcattgg tgtcgtcata cccgcctttg
     7261 ctgtctttca caaatgcatt ttgtaagaaa tcaaatagtt taacatataa cttttgacta
     7321 aatgaatgat gcttttttga ttttcctttg atgtctgcta ataaaacacc tgccaactca
     7381 tcatccgcat ataaagcaat gatttgtgtc gcgcgattca attctagata ccaaaagtat
     7441 cttccataaa gattcaaaag gagtttgttc ttggtatacc aatcaaagtg cataccttta
     7501 atcgcgaatt gaatggcttt tttgtagtct ttcttttcca gttgcttaaa ggctaattcc
     7561 atcacttatt ctcctattct ttaaatattg atatgctata cttatattat aatagacaag
     7621 gtgtcggtta tctatgttca gatagcggtg aaattgtcgg ataaccgaca caattccttg
     7681 attatgttga ttaggaggct gcaaaatgaa aaaagaggat cgccgcgtca aaaaaacaga
     7741 aaaagcgtta gcagaagcat tatcaaaatt attagtagat aaaaaaattc aaaacatcac
     7801 catccgtgaa ctgaccgaaa ctgctgatct tcaccgttcg acattttata cacattatac
     7861 ggatatctat gatctttatg aaaaactgga aaatagcttt ttccaagact tggatgaaat
     7921 catttcctac gatacgacac atagctacga ggatctatac gctcaattga tcgactatgt
     7981 ctatacgaat acgttcgtat acaagttatt cgtcatcaac cacggagata tccaatttca
     8041 aaagagatta aataatctga tcgaagaaaa ttatttaaaa atctggctac aagaagacaa
     8101 aaaaaccgtg atcacagaag aaatgcgtta cttcaccatc taccatatcc aagggtgcct
     8161 ctccgtttta aatcattggg tagaaaagca attttgcata ctctaagaaa aaaatgctga
     8221 ccttcttgcg cttattgaac gacaacatag aacctatcat gccttaaaaa caaacgaaaa
     8281 aggaagcgtc gggacgcttc caattcttta tttaaaagct caaattgatt attaaaaatc
     8341 attaggttac aaacttaagg ttatagttaa agctttctta caatttaata gctgttgctg
     8401 tttctttcaa aacattattc agctcttcga tgttcttgcg tccttgtgtc tgcatccatt
     8461 ctttggcagc atattcacct ttagtaacga atggttctac accattcgcc caagttgcgc
     8521 gcccacagag taccccattg aatgtagagc cagcctcttt tgcaaatttc aaggtttctt
     8581 ggaacaattc agcagttact cctgcgctca agaaaataaa tggcaggttg gtagtctcgg
     8641 attgttcttt gaaataagca gcagcttctt ctttggtata agcaacttca tttttagtgt
     8701 aaccatccac atagttcata ttaacaggaa cttccacttt caaaacgtca actccatagc
     8761 gaggcttaga aaattccttc atcatttcat ttactttatg cggttttttc aaggcatatt
     8821 ccttcgaaga agtatcagga tcgttggcat cataagaaac caattctaag aaaaatggca
     8881 aatcttcagc aacacattca gaaccaatcc gctccataaa cgccttcttt tggtcattga
     8941 tgtcatcaga ttcatctaca tcgtagtata acaagaactt acaagcatcg gccccttgct
     9001 cctttagacg ttttacagac caaatagata aaatatccgg taaacgtcct ggagttgaag
     9061 catcgtaacc agttttttca taggccaaca acagaccagc atttttatcg cgaacttcgg
     9121 ctgccggcaa cccatattct ggatctaata agattgctga agcatatgga gtcaattcag
     9181 aagaaatgat ttttttgaaa ttgatgattt gttcatcggt tgctgattta ttttgaaatt
     9241 taccgatcat ttttttcaag gcaccacgtt gatcgattgc taacgcactg atgatgccac
     9301 tttcattact tagttgacga atataagttt tctttttttc tgttaaaggc atgatttcac
     9361 tacttccttt ttagttttct tctaattttt ggtttaaaag ttcagctatt tcttcagcag
     9421 actgggcttg tttcagacct tctgtcacat ctttgtgcat caaaagtctg gctacctgag
     9481 ataaaaattt tagatgggtt gttccagcct ctgaatctgg aataaacaga gcgattacaa
     9541 aatccgttcc ttctccatct aaacttttcc aatcgacctt accgtttaac cgaacaatca
     9601 caatatcggc atccttgata ctagcatctt tggcatgagg gatggcaaaa ccatccatca
     9661 taccagtcgt gctttcttct tctctttcca tcaatttttc gtaaactgca tctcggtctt
     9721 cactaatgct caagacaatt gactgatctg caaggaagtt cattacctca tcttgcgtcg
     9781 taaaattttt attcataaaa actttatttg ttttgatcaa agtcatttga aatccttctt
     9841 tctagaataa cctttagttg ttggttgctt ctttcatttc aacagcagct gcttctgctt
     9901 cacgtttttc tacactacgt ttttggatca accctaaaat tactccgcca acgattgaac
     9961 caagtaaaat cgaccctacc catgcaacct taccagtaac aacaggtaat actagaaaac
    10021 ctccatgagg tgcgggaact tggacaccaa acatatatgt cagtacagca gcaattgatg
    10081 aacctagcat catagttggc agaacacgca gcggatcttt agccgcaaat ggaattgctc
    10141 cttcggtaat gtgcgttgag cccaagataa agttgactaa accagcgttg cgatcattcg
    10201 tgtcgaaata ttttccaaaa aacagtgcgg caaatccagt aatcaatggg ggcgcgatac
    10261 aagctgccga aacccctgcc ataaatgtat ggttaccttg tgccaacaac gcagtccctg
    10321 taacataagc cgctttattg actggacctc ccatatcaaa tgctgacata cagccaacga
    10381 taatccctaa aattaatgga ttagaatttt ggaatcctgc caagaaattc atcattcctt
    10441 ggttgactgc ttccattgga tttgctaata aagacatgac tacaccaaca aacaagacac
    10501 cgatcaccgg atataagaag atcgccttca gaccatccaa ttgtttaggc aagcctttca
    10561 tgaccttttg caatagtaag ataaagtagc ctgctaagaa acctgcaaaa attccgccta
    10621 agaaaccggc tccgccagaa ttggctaaca gtccgccaac aaacccaaca accaaaccag
    10681 gacgtttagc gattccttca gcgatataag ctgctaaaat aggaaccatc attcccattg
    10741 ccgcatcacc aacacccttt aacatagcgg cagtcggatt gtaagtagga tcattgggat
    10801 caaaagagta cacaccccag atggcaaacg aaattgcaat aattacccca cctgccacta
    10861 caaacggcag catatgagaa acaccgttca tgagattttt ataaatactg tgaccaatgc
    10921 ctgctgcaac ccccatctcc gctttctcac cagcagcgtc aactgttttg ttttctgcag
    10981 tgattgaacc tttaccagcc aacgcatctt cgatcaattg gtcagcatct ttaatcccct
    11041 tgctgacagg cacatcgatt acacgcttac ctgcgaaacg ttctgcttga acatctttgt
    11101 cagctgcaat gattacagcg tcagcgtttt ttatttcttc tggtgttaat tcattctcta
    11161 tccctacttg accatgggtt tcaactttga tagatacccc tttgcgctta gctgcctgct
    11221 ccaatgcttc ttgagccata taagtgtggg caattcctgt tggacatcct gttgcggcaa
    11281 caattttgta agttgtcatt ttttactcgc tccctctttg atttcgattt gtttttccaa
    11341 ctctgcaaca tccgaaaaat ctgttaatcc tttacgaaaa gcggttgaac ttccagctgc
    11401 taaacttttc ttcaaattaa tatctacctg ttcacctttt aaatatcctg caagaaatgt
    11461 tcccaacaaa gtgtcaccag cacacgctgt attgactact ttacctttag gagaattccc
    11521 agaataaacg atatccttat ttaaaaatat tcctccctct cctcctagag aaaggagaac
    11581 atttttcgct ccatcggcaa tcaagcgttc agcataatga atatatcctt cacgatcact
    11641 aagctcgaca ttataccaag attgcaattc ctcctcgttt ggttttaata agaacggctg
    11701 atacggcaga caatcaatta tttccggata gcttgaatca ataatcagat ggaccttatt
    11761 ttcaaaacaa atacgactga tatcaataag aatttcgggt gagacccctc gcggcaaact
    11821 gccagaaaca cacaaataat cgtcagcttg cagcttttgt atttgattta gaaaagtttt
    11881 gatttgttcc tgaggaatct caggtccttt attaaccaat ttgaattctt cattggtttt
    11941 attaacttgt gtaaaaacgt tgattctagt cattcccggt acgctaataa aatcagtgct
    12001 aatatctttt tgatgcaaat aatcctcaat ataattcccg gtaaaacctg ctttaaatcc
    12061 taaagctgta ctatcgatac ctaattgctt catgatcaat gaaacattca caccttttcc
    12121 attagcttgt atatcatcat cgtttgttcg attgacttgg gatggtttca tttcatctgt
    12181 ttcgataaaa aggtcgattg ccaagtttaa agtacatgta taaactgaca tttacgctcc
    12241 tcctttctta catattcatt atgtatcaag atcgaatgta aatgttttca taaatgacta
    12301 aattcattga aaacttttac aaaatatatt aaataaactt gaagtaaagg aaactcccgc
    12361 tttacaatag aattatccta actaatcgga ggcaaaatat ggatacaaaa ttaagtgaat
    12421 cggagaaata tctctgggat tttattcaaa gtcacatact cgaaatcccc tcctattcca
    12481 ttgtaaaact aagtgaatat gccaacgttt caacagcaac cgtcgttcgt actatgaaga
    12541 aaaaaggata tgaggggttt acgtctttta aacattcgtt aaaagaaaaa gaaaatacaa
    12601 atatcaattt tgccgctctt gataaagttg atgaaggaat aaagactgct attcttaaaa
    12661 atgagcaaga ggtaatccgc accatcaata tggtagaaag cgggaatatt gaagatgcta
    12721 ttcaacgaat caaggctgct agaagaatca tgatttttgc ccgaggtttt tcagaattga
    12781 ttgctcagga aatgatggtc aaatttcaac ttaccggtaa atattgtgag atgcatactg
    12841 atcccaatat cataaaaaac attagtagaa aattatccaa agatgacatc gtgatctttg
    12901 tttcactaaa cggtgaaaca aaggaattga ctaccgcagc tgaaaactgt tataaagctg
    12961 agatcgggac aattttgctt actgctaatc gtcattcaag tttgatggaa catatcgaga
    13021 ttccttttgt tggctttaaa tcagaaggct ccttcttccc agattatgag gtacgctcca
    13081 gacttccact ttctatactg gctagaatct tacttgactc ctatgcactg cgaacatcag
    13141 gattcacaca gcactaactc tatatctgta agactgtacc taaaatatat ctgtgtgaat
    13201 taactaaggt tgtctagaaa aatatttaat cgctatttgt attctcgtgc tttctatcat
    13261 tgctccttct ataacttctt agtttcaaga tagaatagtg cattgagact accaggacta
    13321 cttacgcaaa t
//
LOCUS       DACCDF010000076        12672 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCDF010000076 DACCDF010000000
VERSION     DACCDF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.079"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(147..1178)
                     /gene="queA"
                     /locus_tag="IVK51_002196"
     CDS             complement(147..1178)
                     /gene="queA"
                     /locus_tag="IVK51_002196"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP6431761.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKIGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1371..2546
                     /locus_tag="IVK51_002197"
     CDS             1371..2546
                     /locus_tag="IVK51_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP6431762.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESDTPEESKEV"
     gene            2550..3191
                     /locus_tag="IVK51_002198"
     CDS             2550..3191
                     /locus_tag="IVK51_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431763.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3188..4105
                     /locus_tag="IVK51_002199"
     CDS             3188..4105
                     /locus_tag="IVK51_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6431764.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVNNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4102..4773
                     /locus_tag="IVK51_002200"
     CDS             4102..4773
                     /locus_tag="IVK51_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431765.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMVIVTDWLLGILERRLDPA
                     SRTSR"
     gene            complement(4969..5803)
                     /locus_tag="IVK51_002201"
                     /pseudo
     CDS             complement(4969..5803)
                     /locus_tag="IVK51_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016619675.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6004..6270)
                     /locus_tag="IVK51_002202"
     CDS             complement(6004..6270)
                     /locus_tag="IVK51_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6431766.1"
                     /translation="MSNYTVAIKNSATVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6270..6533)
                     /locus_tag="IVK51_002203"
     CDS             complement(6270..6533)
                     /locus_tag="IVK51_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6431767.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            7008..7193
                     /locus_tag="IVK51_002204"
     CDS             7008..7193
                     /locus_tag="IVK51_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6431768.1"
                     /translation="MALNYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7335..9158)
                     /locus_tag="IVK51_002205"
     CDS             complement(7335..9158)
                     /locus_tag="IVK51_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP6431769.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9755..12460)
                     /locus_tag="IVK51_002206"
     CDS             complement(9755..12460)
                     /locus_tag="IVK51_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP6431770.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         3651 a         2636 c         2389 g         3996 t
ORIGIN      
        1 ttcaaggtgt cttttctata tgttttggaa aataaaaaag atgctggtgt catccaccaa
       61 catcaaaaag tatagagctc tttttctttg tcataaaatc tttgtaaagc ttttttgtta
      121 ttcaaaacta gcggcttttc ataaccttat ttcacgaaca tcgcatctcc aaaactaaag
      181 aaacgatagc gctcttcaat cgcatgctgg taagcagata aagtcaattc tcttcctgca
      241 aaggcactaa ctaacatgac taaagtcgat tttggcaaat ggaaattagt tgagaaagct
      301 tctaccactt tgaattcata gccaggcgta atgaaaatat cagtccatcc gctgtctgct
      361 ttgatttctc cattaaattt cgttccaatc gtttctaatg ttcgaattga agttgttccg
      421 acagccacga ttttcccgcc atttttacgt acttggttca atcgctcagc tgcttcttcc
      481 gtcaaacggt agaattcact atgcatatgg tgttcttcaa ttttatctac gctgacaggt
      541 cggaatgtac ctaaccctac atgtagtgtc agatagacta attcaacacc ctttgcttcg
      601 atctcatcaa gtaattcttt cgtaaaatgc aatccagctg ttggcgctgc agcagaacca
      661 ttttctttcg catagacagt ttgatagcga tctgggtctt ccagacgttc tttgatatat
      721 ggaggcaatg gcatttctcc caatgattca agaatctcta gaaaaattcc ttcatactca
      781 aagtcaatga tccgtccgcc gtgttccaat tcttctttca ctaccgcttg caatctgcca
      841 tcaccaaaag agattcttgt cccaactttg gcacgtttcg caggttttat caatgtctcc
      901 cacgtatctc cttgtgtatt cgttagaaga aggacttcta aatgcgcacc agtatctggt
      961 ttttctccat ataaacgagc aggcagaact cgtgtattat tcataactag cgcatcacca
     1021 ggctgtaact cttcaatgat ttcatggaaa tgtttatctt ccatctctcc aattttccga
     1081 tccaaaacaa gtaaacgaga atgatcgcgg tcttttaatg gtgtttgtgc gatcaactct
     1141 tccggcaagt caaaatcaaa atcttctgtt gttaacatat cgtcactcac tttctatcat
     1201 gtgcttcatc attctatcat gtttttcatt ctgttggcaa aatcagcact ttttcacttc
     1261 tattttttgt tttcctaaat aaaaaaacta ccctatttgt attttttcat tgttttaggc
     1321 atactagtaa tagatagttc ttcattgaaa atagaaaaag aggtggcacc atgattgaat
     1381 ttcaaaatgt gtcaaaaatt tataaaggtg gaaaagtcgc tgtagaggac gtcaatcttt
     1441 catttgaaaa gggtgagttc atttgtttta tcggtacgag cggaagcgga aaaacgacga
     1501 cgatgcggat gatcaaccga atgactgatc caacacaagg gaaaattttg attgatggaa
     1561 aatccatcca agaaattgat ccagtggaat tgcgacgtca gatcggctat gttatccaaa
     1621 gtattggatt gatgcctcac atgacgatcc gtgaaaatat cacccttgtc caaaaattgt
     1681 tgaaagtcag tcaagaagaa cggaataaaa cagctgaaaa aatgattgat cttgtggaat
     1741 tgccccgtga gatgttagat cgctacccac atgaattatc tggtgggcag cagcagcgta
     1801 ttggagtcgt tcgtgccctt gcagcgaatc aagatatcat cttgatggac gagccattcg
     1861 gtgcgcttga tcccatcact cgtgattctt tgcaagacct tgtcaaagat ttacaagagc
     1921 gcttgggaaa aacgatcgta tttgtaaccc atgacatgga tgaagcgatt aagctagcaa
     1981 gccgtatcgc catcatgagt gaaggccgtg tgatccaatt tgatacacca gaaaatatct
     2041 tacgtcatcc tgcaaacgat ttcgtagaag agttgattgg agaagatcgc ctactacaag
     2101 ccaagccaaa tgcgacaaga gtcggagaag ttatgctaaa tacagcaatc acgattacac
     2161 cggaaaaatc attacaagaa gcaatcaaac tgatgaggga aaaacgtgta gatacgttgt
     2221 tagttaccga taattcgaat gtattaaaag gatttatcga tgtagaaacg ttgaataaaa
     2281 agcgaggaaa agtatccagt gtcggcgaca tattaaataa acatgttttt tacgtaaaag
     2341 aatccgccta tcttcgtgat accttgcaac gcattttaaa acgcggactg aaatacgtgc
     2401 cggtcgttga tgaacagaac aaagtcgtag gtatcctgac tcgtgcatca ttagtggata
     2461 tcgtctacga tgtgatttgg ggagaagatc ccgcaactac tgatgttgca gagtcagata
     2521 ctcctgaaga atctaaggag gtctgaccta tgcagcagtt ttttcatcag cacggttcag
     2581 aaattctctc aaaaggtttt gaacatcttt atatttcggg acttgctttg atcattggta
     2641 tcatcattgc tgtaccgctt ggtgtcttac tgacacgttt tccaaaagct gccacgattg
     2701 tgattggctt aaccagtgcc ttgcagacaa tcccttctct tgctttattg gcattgatga
     2761 tcccgctatt tggcgtaggt aaattacctg cgattatcgc actctttatt tattcattat
     2821 tgccgatttt acgtaatacg tacattggga tgaaaaatgt cgattggaat tatcgggatg
     2881 tggcaaaagg catggggatg actgagttcc aatccatttt ttccgttgaa ttgcccattg
     2941 ctatgccaac gatcatggct ggtattcgtt tagctgccgt ttacgtgatt gcatgggcta
     3001 cacttgcctc ttatatcggc gctggtggtt taggcgattt gatttttagt ggactgaata
     3061 attaccaacc agatctcatt ttggcaggta cgatccctgt aacgatttta gcactattgg
     3121 ctgattggct tctaggtctc ttggaacacc ggctgacacc gatcgcactt agagaggaga
     3181 atgaagaatg aaacgtatac taaaaatgac catggttctt ttctctcttc ttatacttgc
     3241 gagttgttct ttccctggtc ttgccagcaa tacagatgaa gacaccattt cgatcacagg
     3301 tggaatcact tcagaagcac agatcttagc aagcctagtt gccggaatgg ttgaacatta
     3361 tacagataaa aataccgcaa tcatcaacaa tctggctacc acaactatca accatcaagc
     3421 gatgatgaac ggagatgctt caatttcagc ggctcgctac acaggaacag atttaacgac
     3481 tacattgaac cttcctcctg aaaaagatcc gaaaaaagcg tttgctaccg tgaaagatga
     3541 atttgagaaa agatacgatc aaacgtggtt cccgtcctat ggttttgaaa atacgtatgt
     3601 ctttcttgtt cgaaaagaca ccgcacaaaa ataccattta tctaaagtca gcgatctgaa
     3661 aaatgtagcg gatgaattag tagctggtgt agatacttct tggatcaatc gtaaaggaga
     3721 cggttatgac ggcttccaag aaacttatgg cttctccttc aattcgatat tacctatgca
     3781 gattggactt gtttatgacg cagtcgaagc aggaaaaatg gatattgttc ttggctactc
     3841 tacggatgga cggatcgcca gttatgatct agtcatgttg gaagatgatc gtcaattctt
     3901 cccaccatat gatgctgctc cagttgtaaa caacaaaata ttagctgata cgcctcattt
     3961 aaaagaagcc ttaaataagt taggtcatac catcagtacg gaaaaaatgc aggaattgaa
     4021 ctacgaagca gataataatc tagtggaacc atcagtagta gcacaacgtt tcttacaaga
     4081 aaaccactat tttgaaggga agtgaagcaa ttggataaaa tgaatgtctt tcaacaattt
     4141 ttctattatt tccaggaaaa tggctcctat atctttgcac aatttatccg tcacttcctg
     4201 atttctatct atggtgtcct ctttgcagca gttgtcggga ttccggttgg gatcatgatt
     4261 tctaggagac gtaaattagc aaactgggtg attcgtttgg cgaatatcat ccagacgatc
     4321 ccttctctag ccatgatctc cattttaatc attggattag gcctgggagt caatgtcgtg
     4381 attgttactg tatttttgta ttccctctta ccgatcatca agaatacgta taccgggatg
     4441 atccaagtag acaaaaacat ccttgatgtc ggcaaaggca tggggatgac tgctagacaa
     4501 cgactattca tggtagagtt accactctct gtttctgtta tcatggcagg cataagaaac
     4561 gcgttagttg ttgccatcgg gataaccgcg atcggtgcat tcgtcggtgc tggtggtctt
     4621 ggagatatca ttatccgagg aaccaatgca acagatggca catcgattat tttagcaggt
     4681 gcattgccaa ctgcattaat ggtgattgtc acggactggc ttttaggtat tttggaaaga
     4741 cggctagatc cagccagccg tacatcacgc tgatgttatt gtaaaaaaca aaagcttctg
     4801 cgaccatttg ttggtcgcaa aggctttttt agttctgtca ttttttaagg caataatcca
     4861 ctctgattct tccctcctta tgggcaaaga gttgcttttc ttagctattt atccattccg
     4921 tatactccaa cttcatactg ccaacgggtt catctctgca tccaccgact aagttttttt
     4981 cttatccgct aaggcatttt gccaatccat tttcccatca ctcgtcatgg aattcatacc
     5041 acatgatctc taaatactcg tctgcttgtt ctttgaattg ggcatcaatt cttacatcct
     5101 ttggcgtggc tttcatttaa gccttttcca tcagtcctcg attgttcgat gccttcagct
     5161 acatcatcaa aactggtgac tgccgtatcg gtaacttcga tcatctttta tacaatcggg
     5221 aacttgccat cctcatcctt ggctaaaaat atatctagcc attccagctt ctcgccatgg
     5281 gaggtttgcg gttcctaaaa ccggtacatg attcttatgg actgcatccg tcgcaactag
     5341 acttggcggt acgatgatcc ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat
     5401 attgccaata agaaaagaca ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt
     5461 tgatactgct attcatggcc gaaagcgcca cgactttgac ttcttccgtt tgagtgacat
     5521 tcatgttcgg caatgtcgct ttggataccc gctctgctaa agaaacgaca ctggtattgt
     5581 atttcgtatc tgtatctttt ccaaaagacc atgccaacga atcctctggg aaccaataag
     5641 acgactccgg ttgattgtcc attgcttttt tcaacatact tttggctgtt tctcgataaa
     5701 cgacatttcc aattgtcttc gcctcacttt ggcgataatc gatggtcgtt gccactgcca
     5761 acactaacaa cgatgctcca attttactga ttttccttgt cacacttctt cctccttgtt
     5821 ttacataaac ccgtcaacat tccattccta aactcaaaca atgacccttc aaaggtggag
     5881 atataggaac aaattaaaga aatcatgcaa taaatccaag aaggttggtt ttagaacaaa
     5941 aaaagagtct cctttttggg gagactcttt gttaactatc taaaaaggtt ggctcttctc
     6001 acttcactca taatgtgtcc aggcagaata aatttccaca actttatttt cctcatcaac
     6061 tttgtaaacc actcgatgct gtctattcag cctgcgtgag tatctgcctt catgtgtagg
     6121 tctcagcttc tcaaaagact gcgtgggcat atatggatct tccttcaatg tctgaatcac
     6181 ttcttcaaat tgtttcttta aattcgattg cttgattttt cttaaatcca ctgtcgcaga
     6241 gtttttgatt gcgactgtat agttactcat caaagagctt cccaatcaat atcatcgata
     6301 ttcgtaaagc cactgtcgtc tttttcgcgg tctcgtactt tatctaaagt acctgtttgc
     6361 tcaagaaaaa gggtttcttg aatcgctctc cagtcttcta gcccaatcaa tacagcattg
     6421 tttccactac gatcactaat aatctcgatt tcagtatgat tttcatttac ctctttcaat
     6481 aactgataaa aatttttacg agctgtgcta ggattcatcg ttgtattagc cattgtctct
     6541 cctccttttt ctgatataat tataacgtac ttgattaagt acgtcaaaaa atacaataaa
     6601 gcacttaaat tcagcaaact tttcctcttt tttacataga gaaaaagacc cctttttagg
     6661 gaggatcttt gtccacgctc acttcttact cctttacgac tagcggtagc caaacgtaaa
     6721 gtaattggta acaacgtatc atctttagca aaggtcgaat ccaaagcctg tgagtctatg
     6781 caacaacacc aatacacgaa ggttcctcgt tgcagtgtga cttgattgat atcttgaata
     6841 acctgaggat acccacgaaa gatttgtaac ggttattcaa aactgatttt ggccgtcaag
     6901 tcttctactg cctcaaaaat gattgcctct ttcgtctcct gccattcatg ttcactatcg
     6961 atttgatact tgcttagacc gtcgggtttg tgaatcacca attaaaaatg gcactgaatt
     7021 atggtaacaa aaaatggtca cttgacgagg ctgagatttc tacttatgaa gacacatggc
     7081 acttaagagt cgaggtaaag gaagaagctg aaaatatagg aggctgtaca ttatttggca
     7141 aaggattcgg aaattatgat caagacatca aaatcaaaat gttttacagc taaataggaa
     7201 gaataataac atttcatacg ctgttcccat ctttaatggc ttatagaggg cacctttagc
     7261 tgggaataag acaaaacaga ccctctagct tcaattaaga gctagagggt ctgtttttga
     7321 ttttaaaact tggtttattt cttcaaatgg tagctatagg tgctgacaac gatattttca
     7381 cgccaagaca atcgcaatct taaccgcaaa cttgtttggt tatggagaat attctgccag
     7441 cgtctgtttg gtacaaattg aggaatcaac acagtagttg tatagttttg tttcgctgca
     7501 tttttactta cgatatctac ataacggata attggatttt caatcgagcg ataagaagag
     7561 tggacgattg aaaaacggac atctgggaaa tgctttttaa attctgcttc gatttctttt
     7621 tctttttcga catcttcgtc tagcgaaaca tgcatcgcaa cgacataatc accgatcgaa
     7681 cgtgcgtaat tcaacgcacc gatatttacc cgtgtcacat tcccgactaa tacaagaacg
     7741 gtattcccgt cataatcatg tagttttgca tcattttcta aacgaagctg ttctgctact
     7801 tttttataat gatcatggat cttataaaaa atgaacataa cgattggcat aatgatgaag
     7861 aaaggccaaa tgtcccctaa ccgatagacg aacaaaatcg caatgattgc atatgagatg
     7921 aacgcacctg taatattggc aatcgacttg cttaaccatc tttttccttc ttttttccat
     7981 ttgacgacca tccctgtttg agataaagca aatggaatga acacgccgat agagtataac
     8041 ggaatcaagc gttctgttga gccatggaaa ataaacagca atacgatcga acctagcgct
     8101 agtgtgataa tcccattgga atagcctagt cgatccccac gatcttgata catgtgaggc
     8161 atgaatttat cttttgccag attgtatgca agaactggaa aagcagaaaa accggtgtta
     8221 gcagcaactg ctaaaatcag cgctgtagca aattgaagga cgtaatacaa aataccatgt
     8281 ccaaagacag ctttaccaat ttgagacaaa acagtgactt ctttttcagg aacgatccca
     8341 taccaatagt tgataaatgt gatccccacg aagaagaaac ctaagatcaa tgccatcatc
     8401 gtcaatgtag cagcggcatt ttttgctctt ggcttcttaa agaatggtac agcattacta
     8461 atcgcctcca ccccagttaa agaggaagaa ccagaagaaa atgctcgtaa aatcaaagcg
     8521 atagaaacac ccggcacagc cgctcctgga agtgcagtag cttgcaatgg cacagcacct
     8581 gtcacgatat tatataaacc aacaacgatc agtaacgaga tcacaaggat aaatgtgtac
     8641 accggaaata ataggaacga tgcagattcc ctcagcccac gaagattcag catcatcagt
     8701 agcaaaacga ttgtcacaga aatagcgact tgatgaccgt agagtgctgg caccgctgat
     8761 gtgatcgctt cagcacctgc tgagacagat actgctactg tcaacatgta gtcgatcaat
     8821 aaagagccac cggaaatcaa gccagcattc tttccaagat tctcactgct gacgacatat
     8881 gctcccccgc catgaggata agcatggatg atttgacgat atgatagcgt taatgaaatc
     8941 aataaaataa taacaaaagc ggcaatgggt agcgaatacc agatggctgc agcagacaaa
     9001 gcgactaaaa cgacaacgat ttgttccgta ccatatgcaa tagatgataa cgcatcagac
     9061 gacaataatg ccaaagcagc aaatcgggtt aatttgtgct catcattttc agcagatttt
     9121 aaaggcttac ctactaacaa cctttttaag taatccaatt tctaaactcc tttacacaca
     9181 attttacttc aagttttata gtcgaaaatt tatagtaaac aagatgttat agtacgctca
     9241 ctgaccttaa aagtcaataa actttaagaa aataattgga aaaaaacaga aaaatatcaa
     9301 gcgctttttc aaacgcttta atacagcaaa aatctaacat ttttttatgg gctttttctt
     9361 tgaattctat aataaaagga tagaacttga cttagaaaat caagttctat cctttttgat
     9421 atcttaactt ttgaaagaga gatgtaaggt tgctttctac ccatcattct tactactcga
     9481 agcatagcgc agttgtcaca acctcttgat aaacggtgtt taaaagcttg catctgcggt
     9541 aataagccca accaaattaa aaacataaga agctgttttt atttggtcgt tcttattact
     9601 tgatgcagaa cagccttttc acaacctctt ggtacacggt gttcaatcag ctgaccctcg
     9661 gcattagctc aaaaaacggc aaaatatgag gaactatttt tccatttttt tctctaatac
     9721 tcaggtcaaa acgctgattt cacaacctct ttttttaatg aaaaatatgg ctctcatgcg
     9781 ccgcttcttt gaatttcgtc attttttctt cgatgaacgg atagattgcc gttcctacga
     9841 ttccaaagac aacaaacaga ccgatcatga tccagattgc tttcgtagca tttggccagt
     9901 aaatgcctcc tgcagattcc cgtaacaagt ttaccgcatg agtaaatggc aagaacggat
     9961 tgatcatttg gaagaatttc cctgagactt ggatcggata gttcccgcct ccgccagaga
    10021 tcgacagtac taagatgata atagcgatcc ctttaccgac attatcgaat aatgcagcca
    10081 agacatagac gatcatcatg aacgctaagg cgatcaagac agcaaatgac acgctgtaga
    10141 ccggattttg gacatctact cctagtaaga aaatattccc taatgtaacg atcaagcttt
    10201 gcgcaatcgc cattgtcaaa aatgtcagca tacgagcaac gaattgttct cgtttcgtaa
    10261 aattgtttcg gtctttcttt cctaaatagt attctgtcgt agcaacactt gaaagaagaa
    10321 cagcgccgac ccacagacat agtgcagtat aaaacggggt gctagctgaa ccgttatttg
    10381 cgattggata aagcgcattg gtttgtagct caacaggttg tgtaaagaag tcactttctt
    10441 ttgttgcatc tagtttcaat aatttgatga cttcgcctag atcgacttct tgttcgcctt
    10501 ttcggatcgc gtctgctgct ttttggatac cagctttgat atttggccag tcattgttga
    10561 tcaagtccgc agcagcgttc acagctgttt cgacttctgg cattttttca tttactgttg
    10621 tcaatgtacc ggtgatttca ttttttagtg ctggccaatc attttggata aaatcagcgg
    10681 ccaatcctaa tttttgctga aggatcggta attcattgtt gtataggtct gccccttcat
    10741 tgatgccatt gacgatggta tccatatttc cattcagcat tgtattggca tcatggattt
    10801 cttgtccaat tgcaggcatt tctttttgat atttttctaa aattgccact gcattcgtta
    10861 cagttgcttt cgttgaactg agaagcgaat caaagtcgat ctggtccgct ttgcttagcg
    10921 ccccttgcgc agtcccaatc gtatcgatga cttggtctaa tagagaagtg acagtggaac
    10981 tgatttgacc tgtattgata ccagcaatga tgccattgat attattaaca acggcatcaa
    11041 tttgatctag caattcatca atagccttcg tgtcttcatt ttgaacagct gtgttcaaag
    11101 cagataggcg gttttgcaga ttgcttagca agggttttaa ttgatttagc tgatcaataa
    11161 ttggctgcaa tttcgtattt ccagcacttg tttgcaattt agtcaggaac tcgaccagtt
    11221 tatcgattgc attttgttgt tgagatattc cgtcaataaa gccgttcaat acattgctga
    11281 tgtttttctt ttcatcatca gtcagcttac catcttcaat agctgttctt acagtatcta
    11341 ttaacgaacc tgtcgttgtt gccacttgtc caattgattt caatgtaaca tctacactgt
    11401 ttgtgatgct tggtatcgca tctttcagct gagaggcacc tgattttgtt tggtttgcta
    11461 gatcacttgc ctgattttct aaatttttca catctggcaa gatatcctgt acttgttgga
    11521 tgatcgtcaa gccttgtttt gcttcatcga ttccttggtt cattgtctct tggatcgaag
    11581 caaaatcgcc gtcgatctct gctagttgtt taccggcatt ttggatctct gggatttttt
    11641 cttgcaaatc aaggatgatt tttgcttgat ctttcaactc aggcattttg tcattcaatg
    11701 caacgacttt ttcacccatc tcatctactt tagggatata atccacgaat tcatttgctt
    11761 tattcagttt ttccttgatt tcaggtagtt gactttgtaa ttcgaggact tgttttgtgt
    11821 agccgtcgat cgtgtccaaa ttttcatctg ttgataaaat catatctttg actttattga
    11881 tacttactag gttcgtatcg atgtcgtaac ctatctcatt gaatactttc aataaagtcg
    11941 aactggctgt tttgataaat tcatttgtga tttgcgattg gatcgaactt gctcctttat
    12001 cggtgatttt cggtgcaatc gcattgattt tttcatttac cgtatattcg atttttggtt
    12061 tcttgatatc accactagta aaactcagca gatcttctga gaaatccttt ggcaagtaaa
    12121 tacctgcgta atactttccg gaacggactc cgtcttctaa ttgttctttt gaatcaacga
    12181 attgccatcc taattgttta tttttatgaa gcgattcgat gacttgctcc ccaatggcca
    12241 cttctttacc ttgaaactct gctggcttgt ctgcactgta aacagcaatt ggcagctctc
    12301 cagtatttcc atatggatcc cataatgctt tgatgttaaa ccacgcatat agcgaaggga
    12361 tgatcatgat cgcgatgatc agaagtgttg caatcgggtt tttgaaaatc cgtttccagt
    12421 caagcaaata taatcgaagc gtatttttga ttgactgcat agttctcccc cttcttcttt
    12481 tttcagctga attactttac catgtatttc atactttttt caatcggctt ttttcaaaaa
    12541 agtcgaattt ttggacatat cacggcaatt gtctcattgt aatctcttac atattgagaa
    12601 ataattaagg attacagact ttactttatc atgaaataaa agggaaagat aattttatgc
    12661 caaagttttt at
//
LOCUS       DACCDF010000077        12670 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCDF010000077 DACCDF010000000
VERSION     DACCDF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.080"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..305
                     /locus_tag="IVK51_002207"
     CDS             6..305
                     /locus_tag="IVK51_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6431771.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVK51_002208"
     CDS             378..1739
                     /locus_tag="IVK51_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6431772.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVK51_002209"
     CDS             1761..2021
                     /locus_tag="IVK51_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431773.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVK51_002210"
     CDS             2035..2787
                     /locus_tag="IVK51_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP6431774.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVK51_002211"
     CDS             complement(2881..3486)
                     /locus_tag="IVK51_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6431775.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVK51_002212"
     CDS             complement(3539..4513)
                     /locus_tag="IVK51_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP6431776.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVK51_002213"
     CDS             complement(4891..5670)
                     /locus_tag="IVK51_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6431777.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVK51_002214"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVK51_002214"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP6431778.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVK51_002215"
     CDS             7161..8558
                     /locus_tag="IVK51_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6431779.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVK51_002216"
     CDS             8639..9079
                     /locus_tag="IVK51_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6431780.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVK51_002217"
     CDS             9227..9553
                     /locus_tag="IVK51_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6431781.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVK51_002218"
     CDS             9554..10303
                     /locus_tag="IVK51_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431782.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVK51_002219"
     CDS             complement(10383..10838)
                     /locus_tag="IVK51_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP6431783.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVK51_002220"
     CDS             complement(11265..11540)
                     /locus_tag="IVK51_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431784.1"
                     /translation="MKLEDKKVILKKIKRLPEQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVK51_002221"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVK51_002221"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP6431785.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2176 c         2586 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttgctct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACCDF010000078        12573 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCDF010000078 DACCDF010000000
VERSION     DACCDF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.081"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1014
                     /locus_tag="IVK51_002222"
     CDS             <1..1014
                     /locus_tag="IVK51_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6431786.1"
                     /translation="ELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQGTFI
                     DYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLNDTW
                     LVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDP
                     FTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQLVS
                     AHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGDINA
                     QILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQ
                     ERE"
     gene            1025..3229
                     /gene="topB"
                     /locus_tag="IVK51_002223"
     CDS             1025..3229
                     /gene="topB"
                     /locus_tag="IVK51_002223"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6431787.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            3470..4063
                     /locus_tag="IVK51_002224"
     CDS             3470..4063
                     /locus_tag="IVK51_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP6431788.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4076..4975
                     /locus_tag="IVK51_002225"
     CDS             4076..4975
                     /locus_tag="IVK51_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6431789.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            4978..5451
                     /gene="ssb"
                     /locus_tag="IVK51_002226"
     CDS             4978..5451
                     /gene="ssb"
                     /locus_tag="IVK51_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6431790.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFANQNGNR
                     EADFINCVIWRKPAETMATLAKKGGLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSTTSPSPTTFEQRDTNNGLDASQNPFGGQSIDISDDDLPF"
     gene            5817..6077
                     /locus_tag="IVK51_002227"
     CDS             5817..6077
                     /locus_tag="IVK51_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431791.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDQFTRLMGI"
     gene            6522..6728
                     /locus_tag="IVK51_002228"
     CDS             6522..6728
                     /locus_tag="IVK51_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6431792.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            6728..6979
                     /locus_tag="IVK51_002229"
     CDS             6728..6979
                     /locus_tag="IVK51_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431793.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTTETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTELEN"
     gene            6995..7411
                     /locus_tag="IVK51_002230"
     CDS             6995..7411
                     /locus_tag="IVK51_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431794.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGACYIMK"
     gene            7393..7665
                     /locus_tag="IVK51_002231"
     CDS             7393..7665
                     /locus_tag="IVK51_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431795.1"
                     /translation="MLHHEVIRRIQKDFSCMEEMKYFVQNVEEQLYLESETSQTKVTV
                     SHNRLDVYKRRPESLKKGAELLRLETEKYVMNVYLEKSELVENFMD"
     gene            7832..8233
                     /gene="tnpA"
                     /locus_tag="IVK51_002232"
     CDS             7832..8233
                     /gene="tnpA"
                     /locus_tag="IVK51_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6431796.1"
                     /translation="MSEIHYGRGYVYSIQYHLVWCVKYRHDVLYGQVDIDVKQFLKQI
                     ADDNNIKIIEIESDKDHIHLLIECTPQHYIPSIVKVFKGASARLLFKKHPELKQLLWG
                     GHLWNPSYFVATVSENTEEQIRKYIQNQKKK"
     gene            8245..9390
                     /gene="tnpB"
                     /locus_tag="IVK51_002233"
     CDS             8245..9390
                     /gene="tnpB"
                     /locus_tag="IVK51_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6431797.1"
                     /translation="MLQHRAYKFRIYPNQEQEILMTKTIGCSRFVYNYFLNLWNHEYT
                     TTGKGLSYNSCSAMLPQMKRNAETIWLKEVDSIAIQSSLKNLSDAFSRFFKKQNKRPQ
                     FKSKKHPIQSYTTKNVNNSIEIRNNFVKLPKLGFVKFAKSQEPKGRILNATIRKNSSG
                     KFFVSILCEEEIVEFPKSNSAIGIDLGITDFAILSDGQKIDNNKFTSKMAKKLKREQR
                     KLSKRALLAKKKGVNLFEAKNYQKQKRKVARLHEKVMNQRNDFLNKLSTEIIKNHDII
                     CIEDLNTKGMLRNHKLAKSISDVSWSSFVTKLQYKANWYGRKIIKVDKWFPSSQICSN
                     CGHKDGKKSLEIREWTCPVCHAHHDRDINASINILTEGLRLHVMGLA"
     gene            9628..9891
                     /locus_tag="IVK51_002234"
     CDS             9628..9891
                     /locus_tag="IVK51_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP6431798.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            9885..10235
                     /locus_tag="IVK51_002235"
     CDS             9885..10235
                     /locus_tag="IVK51_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6431799.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(10259..10873)
                     /locus_tag="IVK51_002236"
     CDS             complement(10259..10873)
                     /locus_tag="IVK51_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6431800.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            11323..>12573
                     /locus_tag="IVK51_002237"
     CDS             11323..>12573
                     /locus_tag="IVK51_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6431801.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         4586 a         2063 c         2488 g         3436 t
ORIGIN      
        1 gaactaaagc caaaacatga acggcaaaat ttcttagttc gagacgatcg tttagatcat
       61 gcggtcgcct ttttatggaa agatccacag acaaatgaga cagtaggggc gagttatcaa
      121 ggaacgttca ttgattatga acgttttggt gagagaggca catataagca tatagataaa
      181 aattcgacag cgaatcatgg ctttaaccta aagatcggtg accctaagca gttaaaattt
      241 tttgagagta gtattgattt acttagctat gctgcactaa atcgtgatca gttaaacgat
      301 acgtggttag tttcgatgga agggttaaaa caccatgtga tcagtcatta ttttggtgag
      361 gcagtatctg aattgagaaa gaaacaagcc tttcctcaat ccatcgagat ttgtgtggat
      421 aatgaccgtg caggacatat tttctacgaa aaagaacaat taatgggcgc agttgatcca
      481 tttacgaatc agaaagtacg ttgtgaacga ggaatcgcta atgattggca agtgccaaaa
      541 gaatacaaag tcatttatga agaagtcgca aaagaagaaa agatcacacc agaagctatt
      601 atggccatac acaagacaga aaacaatctg caattaacga accaattggt ttccgctcac
      661 aaagtaaatg cgtcttttgg tcagcagctt tctgttaacg attcaataga agctattaat
      721 ttgaaagata tttgtagaga agtcgcaaaa gaattaaaag cttgtgaacg cgtggatggt
      781 acgtacgatt ttgatcgttt ttatcaagaa aaaggggata ttaatgctca gattcttttc
      841 tcttataaag cagaacaata ctacaaaggc tataaaaatc atgaacatga atttgtacct
      901 gaagtaaaaa aggattggaa tgatcaattg aagcatgaaa tctaccaaca agagatcaga
      961 aagcaaaaac gtgccatgtt gtttcaacaa ggtagacagc aagaaaggga gtgaaatcgt
     1021 catgatgaaa accgttattt tagcagaaaa accgtcacaa gcgaaggctt atgccgatag
     1081 cttttctaaa gctacccgaa aagatgggta ttttgagatc caagatcgat tgttttcagg
     1141 agaaactgtg atcacgtatg gttttggtca tttagtcgag ttagattcgc ccgatatgta
     1201 cgatgaaaat tggaaacaat ggtcattaga gcatttgcct attttcccta cacattatca
     1261 ctatcatgtt cctaaagata agaaaaagca attcaaggtg gtgaaacagc aacttcaatc
     1321 ggccgacacc attattattg cgacagatag cgatcgtgaa ggggaactga tagcttggac
     1381 gatcattcaa caagcaggcg ccgatcatgg aaagatattt aaaagacttt ggatcaactc
     1441 cttagaaaaa gaagccattt atcagggatt ccagcagtta cgagatgcag aagagaccta
     1501 cccaaaattt gaagaagcac aagcacgcca gatcgccgat tggttgatcg gcatgaacgg
     1561 tagcccgctg tatagtttac tcttgcagca aaaaggcata ccaggaagtt tttccttagg
     1621 cagagtccaa accccaacgc tttacatgat ttatcaatta caggaaaaga ttagaaactt
     1681 ccaaaaagaa ccatattttg aaggaaaagc ccaagtaatc gctcaaaatg gcgcatttga
     1741 tgcgaagctt gatccgaatg aaacccaagc gacgcaagaa gcatttgaag attatttaaa
     1801 agaaaagggg gttcaattgg gaaaacagcc aggtacgatc caccaagttg aaaccgagaa
     1861 aaaaagtgcg gcaagtccac gtcttttttc tctatccagc cttcaatcta aaatgaatca
     1921 gctcatgaaa gctagtgcca aagatacttt ggaagccatg caagggttgt atgaagggaa
     1981 atatctaagc tacccacgaa cagatacccc ttatattaca gaaggggagt atgcgtatct
     2041 gttggatcac ttagacgaat acaaacactt tttaaaggcc gaagccattc ctactcctat
     2101 tcatacaccg aatagtcgat atgtgaataa taagaaagtc caggaacatt acgcgatcat
     2161 tcctacaaag acggtcatga cggcagcggc ttttgaacaa ttaagtccac tgcagcaggc
     2221 tatttatgaa caagtgttaa aaacaacggt ggccatgttt gcggaaaagt atacctatga
     2281 agaaacaacg atcttgacgc aagttcagca gcttcagcta aagacagtcg gaaaagtacc
     2341 agtagatcta ggttggaaaa agctgtttgg caaagagagc gacagaaaag aaaaggaaga
     2401 agaaccgcta cttcctaaag tgacaaaagg agaagcggtg acggtagatc tacaagtcct
     2461 tgaaaaagaa acgaagccac cacaacctta taccgaaggt acgttgatca cagcgatgaa
     2521 aacagctgga aaaaccgtgg atagcgaaga agcacaatcc atattgaaag aagtagaagg
     2581 aatcggtaca gaagcaacac gagcaaatat tattgaaact ttgaaacaaa aggaatatat
     2641 caaggtagaa aaaaataagc ttgtggtgac aaataaaggg attttacttt gccaagccgt
     2701 ggaaaaagag ccgctgctaa caagtgccga aatgaccgcc aaatgggaaa gttacttatt
     2761 aaagattggt gagcagaaag gaacacaagc tactttctta gctaatatcc aaaaatttgt
     2821 ctctcacttg ttggaagtcg taccgggaca aatccagtca acagattttg gatcaacgtt
     2881 acaagaggtg aaagcagcca gtgaaaaaca ggaagcaacg cgtcatttag gtgtttgtcc
     2941 aaaatgtcgg gaacaagaag tcttgcttta tcaaaatgtg gcggcatgta cgtctgaagc
     3001 ctgtgatttt aaattatgga caacgattgc caaaaagaaa ctcacagcca cacaactgaa
     3061 agagatcatt caaaacggcc gaacaagcca atcagtcaga ggactaaaag gtcagaaagg
     3121 ttcgtttgaa gcaacgatcg ttttaaaaga agactttaca acaggctttg aattttctga
     3181 aaagaaaaaa acgaactata agaaaagaac acgccgcact acaaaataat gaaggggtta
     3241 tcctatgcga aatacaacac acaaacaaca agaaatcgaa aatcaaaaag caaaaattcg
     3301 tttagaacaa gagaaattac gtgcaaataa aagaagactg caacaattaa agaaccagca
     3361 gcgcattcaa aaagaaatcg aattagatcg atagacgtta atcttgttac aaataaacta
     3421 ctatgatata tttgatgtag tgtatttgta acgaagggag gggaagtaaa tgcaagataa
     3481 actgaaagaa ttgttggcta ttcgcgatgg aaatttatct atgaaggaag ccaggaaatt
     3541 tggaatcgca gcaaccactg tacaaagatt ggtgaatcaa ggaaaattga tcaaagttga
     3601 tcgtggttat tatgtagagg atggtcatgg aatagatgat ttattttggc ttcagcaacg
     3661 attttccagg ggaatcttct ctcatgaaac gactttagat atttaccagc tttctacgaa
     3721 tatgccgaaa ttcattcact taacttttcc ccatggctat aacgttgatc gttcaattac
     3781 aaaagagcag cagttacaat ttcatttcgt gaagaaagaa gtttatgaac taggaaaagt
     3841 agagggaact tcctttcaag gaaatcctat tactatgtat gataaagagc gtacactttg
     3901 tgatatttgg aatccgagat atgaatttga atatgaaatg aaattagaag cattaaaaga
     3961 gtacatggaa gacccattga gagatccaag taaattaaga gattacatga caaaactaca
     4021 tgtagaaaaa gaaatgaaat accacatgca aagcttatat tgatggagga aaaagatgac
     4081 accgacacaa tttgcagcaa aaacaagaaa tttagcaaag agtacaggat taaatgcaca
     4141 gttagtgcaa cggcatttta tgatggatcg tctaattgaa caaatttctg aatcaaagta
     4201 caaaaatgac ttcattttga aaggcggctt tttattagga tcaaaatatg gaatcgatag
     4261 aaggacaaca attgatatcg atacgacatt tagaaatagc aaattaactg aagaaaaatt
     4321 gaagagcatt tttaatgatt taactagaaa acccacaaaa gaaggcattg ttttctctat
     4381 tcaaggaatg aaggaaacaa gagaagccga tttttatcct ggatttcaaa ttaaattggt
     4441 ggctaattta gagaaagtac gtgtaccgtt taaaatggac gtgacaacag gagattctat
     4501 ttatcctgaa gtagatacac actatcacca attaatgttt gaagataaaa aagtggagat
     4561 tccagcttat ccaacagaac aaattattgc agaaaagcta agtgctacgt tcagctttgg
     4621 cacagataat tcgagagcaa aagattacta cgacttattt accattccga aactagaaaa
     4681 tatcgatacg aaagagttat acaaaagtgt tcgaaataca tttacaaaac gtggggatac
     4741 aaaaccgttg tccttttatt atgaaagaga aatggataag atcagaggaa atgactattt
     4801 ggaaaagcag tgggaaaaat atcgctcagt taacacgttt gcgaattcca tttcatttac
     4861 agatacagtc aacgaaattg atcacctaat gaaaagtgtg atcaaaattg aaaatcaaga
     4921 aagaaaacgg cagtttaaaa gatcacaaca aatgaatcaa gaaatggaga gataaagatg
     4981 attaataatg tgacattagt aggacgctta accaaagatc ccgatttacg ttatactgca
     5041 agtggcacag cagtagcgac ttttacccta gccgtcaacc gaaatttcgc gaatcaaaac
     5101 ggcaatcgag aagccgattt tattaactgc gtgatttggc gcaaaccagc agaaacaatg
     5161 gcgacattag ctaaaaaagg cggcttgatc ggtgttgtcg gacggattca aacacgaact
     5221 tatgacaacc agcaaggaca acgtgtgtat gtgactgaag ttgtagcaga taactttcaa
     5281 ctattagaaa gtaaagcagc gacagagagc cgtgcgcacg ctgatcaatc aaccaccagt
     5341 ccaagcccca ctacctttga acaacgggat acaaacaatg gattggacgc ttcacaaaat
     5401 ccatttggtg gacaatcaat cgatattagc gatgatgatc taccattcta agtagcattt
     5461 tctattggtt gcatttgcta cggtttatgt tataatttag tcaacaaaaa gaattttcaa
     5521 tctataacca aaaaccacta gctggcgggc tagtggtttt cttttacgtt tctattataa
     5581 gaattaacgg gtaggcttac tgtctttctt ggcaaattta caatcaagcc atttgcagat
     5641 atagtgcgcg gctacttctg ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc
     5701 tataacctcc tccccttgcc agattaggcc ggggtagcag acagcaagac tattatagca
     5761 taattgaaat attcaaatga ttggattttt gctataattt tagcgaggtg attgtgatga
     5821 acaaatatgt tggtttgctt gataaaatcc gagtaataaa aacgcaaccg ttattggttc
     5881 gctttacttt acaaacgatt catgaatcga ttaattgtgt agttgcagat attgaaatta
     5941 tcgataaatt gctaattatg gatgatggaa aatacaatat tgccgtcaca ggtcacttca
     6001 ataaacgaaa ccagctagtt attgctagca tgtatgttag aaatcccgat cagtttacgc
     6061 gattgatggg catataaaaa aagaagagtt gcaatcctaa tgtaggactg cagctctttt
     6121 tgttttacat agaaacaaga aaaatgaaaa ggccttttta ggaaggcgcg agcgccttct
     6181 caattgaaaa acctctctca ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac
     6241 gttgcacgtt cactaagata aagggtcttg caaagcaaga caaaatagaa aaaacttcct
     6301 ctcaccaaaa ggttcgttcc tgttcgttat ctctttattg atttgccaga ccggcaacaa
     6361 gttgcctttt atcaccaaaa agcgacaagg acaagggtaa atggcacgct gcgcgccctt
     6421 gtccttgtcg cttttcgttg attcattctg gccaacaaat caataaagaa agaactaacg
     6481 aaaggaagta aaacctttcc taagaggagg cagaatcaat catgcgaaag aaatttgatt
     6541 attggggtgt tcccttcagc gaattattcc caaactatca tgcaccacat actgtcgaat
     6601 gcgattgtgg agaaagagcg aaatgtatca aaagctatcg tttgtatcaa tgtccaacat
     6661 gcgggaaaaa atataccttg tcttatggcg attatatttt gattgatgag aaaggaaaga
     6721 aacgttgatg aaaacacaac acaaattatt gactgtaaaa gaagtcgttc aaattttggt
     6781 ggacaaagaa gtcatttctg aaaaagtagc agaagcttta cttattccgc ggtactggac
     6841 gacagaaacc cctttagtag aggatgcacc gactatgcta tttgaagacc tagtgaagat
     6901 tgcaggatat gaactgcatt atcaagaaaa cttggaaact tatgaaaacg aatattggtt
     6961 aaccgaatta gagaactaaa aaggagaaaa aaatatggct caaaattact atgacgaatt
     7021 tgtcaaatta cctttggata aaatggcaca aaaaatggag gatatgacat tcctatacaa
     7081 tgagacacgt gttccaaaaa aacattataa ggaaagattg tcggtagctg tggaagaaat
     7141 gattgaatca ggtgtcgaaa tgaatctcat tgcgacgtat tatcgaacac tggaagaact
     7201 gaaaaagcaa aatgcaaaat ggtttttcca agccttacta tgcttggaag taggcgtcaa
     7261 accaagcaca atcaaacctt ctgaatacca agctttagaa ctaacgtatg cgaaatttat
     7321 cgaaacgaaa aaagctaaga ctgtttcaag tgaatggtta gattattttg aaaatatcaa
     7381 taagtatggc gcatgttaca tcatgaagta atcagacgga ttcaaaaaga ctttagttgt
     7441 atggaagaaa tgaagtactt tgttcaaaat gttgaagaac agttatattt ggaaagcgaa
     7501 acgagtcaaa caaaggtcac agtcagccat aatcgtttag atgtgtataa acgtcgtcct
     7561 gaatcattaa aaaaaggagc ggagcttcta cgactagaaa cagaaaaata cgtcatgaat
     7621 gtttacctag aaaaaagtga gttggtcgaa aattttatgg actagccgag aacacccgtg
     7681 attttaatca tgggatgaat cggattcagt tgtgacctag ctttagctag gttaaacaac
     7741 tgacaaatat aaactaaata atagtgaaat gtcttgcaat aattgatttt gtagaaatac
     7801 atatattaaa ataattgcga ggtgtttttg tatgtcagaa attcattatg gacggggtta
     7861 tgtctattct attcaatatc atcttgtttg gtgtgtaaaa tatcgacatg atgttttgta
     7921 tggtcaggta gatattgatg tcaaacagtt tttgaaacaa attgctgatg ataataatat
     7981 aaaaattata gaaatagaat cagataaaga ccatattcat ttactcattg aatgcacccc
     8041 acaacactat attcctagta ttgttaaagt atttaaaggc gcatccgcta gactgctgtt
     8101 taaaaaacat cctgaactta aacaactcct ttggggtgga catctttgga atccaagcta
     8161 ctttgtagct actgttagcg aaaatacaga agaacaaatt agaaaatata tacaaaatca
     8221 gaaaaagaaa tgaggtgaat ccgtatgcta caacataggg cttataaatt tagaatctat
     8281 cctaatcaag aacaagaaat attaatgact aaaactattg gctgttcaag atttgtctat
     8341 aattattttt tgaatttgtg gaatcatgaa tatacgacta ctggaaaagg attatcttat
     8401 aattcttgtt ctgccatgct tccacaaatg aaaagaaatg cagagacaat ttggttaaaa
     8461 gaagttgaca gtattgcgat tcaatcctca ttgaaaaatc tttctgatgc tttttctcgc
     8521 ttctttaaaa agcaaaataa acgaccacaa tttaaaagta aaaaacatcc tatacaaagt
     8581 tatacaacta aaaatgtgaa taatagtatc gaaattagaa acaatttcgt aaaactacct
     8641 aagttaggat ttgttaaatt tgcgaaaagt caagagccca aagggcgaat cttaaatgct
     8701 acgattcgaa aaaattcgag tggtaaattt tttgtttcca tcctgtgtga agaagaaata
     8761 gttgagtttc ctaaatctaa ttccgctatt ggaatcgacc ttggtataac tgattttgca
     8821 atcctttctg atggtcaaaa gattgataac aataaattca cgtcaaaaat ggcaaaaaaa
     8881 ctcaaacgtg aacaacgtaa gttgtcaaaa cgtgcattac tagctaaaaa gaaaggcgtc
     8941 aatctctttg aagctaaaaa ttatcaaaaa caaaaacgaa aagtagcacg actacatgaa
     9001 aaagtaatga atcaacgcaa tgacttttta aacaagttga gcacagagat tatcaaaaac
     9061 cacgatatta tctgtataga agacttaaat acaaaaggta tgttgcgtaa tcataaacta
     9121 gcaaaaagca tttctgatgt gtcttggtct agttttgtga ctaagttaca atacaaagct
     9181 aactggtacg gtcggaaaat catcaaagta gataaatggt ttccatctag tcaaatctgt
     9241 tcaaattgtg gacacaaaga cggcaagaaa tctctagaaa ttcgagaatg gacttgtcct
     9301 gtttgtcatg ctcatcatga ccgagatatc aatgctagta tcaatatttt gaccgaaggt
     9361 ctaagattac atgtcatggg attagcttag atccaatcat caaaaaccgt aggaactacg
     9421 gggatagctt ggtaaataag agaaaccgct gtgagtaaag aaatacgctt gcaagtatgc
     9481 tctattccca agaatctcgt gactttagtc atgagaggtt caaggattca tggaatgtta
     9541 atctaattta atagaaaagg ataattaagt atatacgttt tttcttatgt atgttatact
     9601 atgtatatac agaaagaggt gtctactatg gaaatgttac ctataaaaaa atggggaaat
     9661 agtaatggcc ttagattgcc aaagtatatt atggaatatc ttggaattca tacagaagac
     9721 aaagtgaaga taatacaaga agaagtaaac ggtcagaagc ggctaattat cgaagcggct
     9781 aatatagaaa atgaattaac aattgaacag ttatttgaaa attacactga tgaaagaaat
     9841 catgtgacga ttcaaaattt aggtgaagca gtaggtaatg aaaaatggtg agaaaaccta
     9901 gacaaggcga tattctatta ttgaatacag cacctcgatc tggacacgaa cagacaggta
     9961 aaagacctta tattgtttta agtcatgata ttattgcgga ttatagtaac gttgttacag
    10021 ttgctccaat ttctagtaca actagaaact atccgctata tgtaaatata aactcagaat
    10081 atgaaatgaa gacaacagga aaagtattat tagatcaact aacgacaatt gattatgaag
    10141 ccagagagtg tatttttttg gaaaaagcgc atgatagttt agttgaggag ttattgatga
    10201 aagttagaac agtttttcaa aaagtaagca aataaaatag aaacagcagc atgatcaact
    10261 attcgtgatt gaccatgctg tcttttttta ttctatatac ggtttgtctc gtaacattgt
    10321 agtcttttgc aatcttagcg atcgcaacac ctttttttag atcctcaacg atgtttttat
    10381 aaacaagacg acgttgggga tcttttgcat tggcactgta tagcttagga cgtcctttat
    10441 aaacaccgtt cgcttttgca atcttaatgc cttgtgcctg acgtcgttta gattcggtac
    10501 gttcttgttc agcgatcatg gccaaaattt gaatgatcaa atctttaatg aatttatcca
    10561 ggagaggatt gccaattgct tcggccatga tcggtagact tgtgatgatc agctgcacat
    10621 ttttcttctt caagtaattg actgaagcga tgatctcgtc gtaatttctt ccaagtcgat
    10681 caatagcttc cacaataaaa atatcttgat ctcggacgaa atccagtgct tcttgaaaaa
    10741 caggtcgatg agtaatcgtt gcgccagatt gtttctcaat gaaaattctc tctgcaccga
    10801 attttttcat ttcttcaatc tgccgttgtt cattttgatc gatggatgag acacgtatgt
    10861 atgcaactct caaaattttt acctactttc tgcaactatt taaaaaacac cctaatcata
    10921 cacgagaaaa ccagattttt caatgtcgcg gtcgtgtcta gttagggtat accttatata
    10981 gacaaaaact aatgatggag gaaatagtat gagaattttt tgtaagtgtc tactgataac
    11041 ttattctcaa atgttgtaag gattttattc atttgttaat attaaagaac ggaactccaa
    11101 aaaagtccgt actctaattt gcagtctctc actcttttag agtgagagtt tttattagat
    11161 tttcaaatta attagtaaag tgatagaata aattgatcat acaaatcaaa agaagaagca
    11221 gggtgatagc atacctatgc ttcttttttt atgtatttag ctatttcttc tttacaatat
    11281 cgaacatatg tttgtataat gtgaaagaat ggagtgttat ttatgatgga ttattcaaaa
    11341 gagccagtaa atgattactt tttaatcgat atgaaaagct tttacgcaag tgtcgaatgt
    11401 atagaaagaa atttagatcc attaacaaca gaacttgttg ttatgagtcg agctgataat
    11461 actggttcag gattgatatt agcttcttct cctgaagcaa aaaagcggta tggtattaca
    11521 aatgtgagta gaccacgtga cttaccacaa ccatttccta aaacactaca cgttgttcca
    11581 ccacgtatga acctatatat caagcgaaat atgcaggtaa ataatatttt cagaagatat
    11641 gtggctgatg aagatctact gatttactcg atcgatgaat caatcttaaa agtgacccga
    11701 tcactgaatc tttttacgac tgaaggaaca cgaagtcaac ggagaaagaa gctggctcaa
    11761 atgatccagg agaatattaa agaagagcta ggattgattg ctacagtagg tgtcggagat
    11821 aatccccttt tggctaaact agccttagac aacgaagcaa agcataatga aggatttgtt
    11881 gcggaatgga cgtacgaaaa cgttcctgaa aaagtatgga atattcctga aatgacggac
    11941 ttttggggaa ttggctctcg aatgaaaaaa agactcaatc agatggggat tatgagcatt
    12001 agagatcttg ctaactggaa tccttatacg attaagaata gactaggtgt gattggctta
    12061 cagctgtact tccatgctaa cggaatcgat cgaacagata tagcaatccc gccagaaccg
    12121 acaaaggaaa aatcttatgg taattcacaa gtgttaccac gagattatac tagaagaaat
    12181 gaaatcgagc tggtggtaaa agaaatggcg gaacaagtag ccattcgtat caggcagcat
    12241 aattgtaaaa caggatgtgt tcatttaaac attggtacgt cgatcttaga aacgagacca
    12301 ggattttcac accagatgaa aatacctatc acagataata caaaggaatt gcagaattat
    12361 tgtcttttct tgtttgataa gtattatgaa ggacaagaag taaggcacgt tggaataacc
    12421 tatagcaagc ttgtgtacac cgattcgctt cagctggatt tatttagtga tccacaaaag
    12481 caaattaacg aagaaaattt agataaaatt attgacaaga ttcggcaaaa atatggtttt
    12541 acgtcgattg tacatgcttc ttcaatgtta gaa
//
LOCUS       DACCDF010000079        12112 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCDF010000079 DACCDF010000000
VERSION     DACCDF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.082"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            126..1151
                     /locus_tag="IVK51_002238"
     CDS             126..1151
                     /locus_tag="IVK51_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP6431802.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1190..2140
                     /gene="manA"
                     /locus_tag="IVK51_002239"
     CDS             1190..2140
                     /gene="manA"
                     /locus_tag="IVK51_002239"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP6431803.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            2234..3937
                     /locus_tag="IVK51_002240"
     CDS             2234..3937
                     /locus_tag="IVK51_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431804.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            3939..5972
                     /locus_tag="IVK51_002241"
     CDS             3939..5972
                     /locus_tag="IVK51_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP6431805.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            5983..6423
                     /locus_tag="IVK51_002242"
     CDS             5983..6423
                     /locus_tag="IVK51_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431806.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6623..7750)
                     /locus_tag="IVK51_002243"
     CDS             complement(6623..7750)
                     /locus_tag="IVK51_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6431807.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(7753..8430)
                     /locus_tag="IVK51_002244"
     CDS             complement(7753..8430)
                     /locus_tag="IVK51_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431808.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            8694..10460
                     /locus_tag="IVK51_002245"
     CDS             8694..10460
                     /locus_tag="IVK51_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP6431809.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10475..10912
                     /locus_tag="IVK51_002246"
     CDS             10475..10912
                     /locus_tag="IVK51_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431810.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            10925..12076
                     /locus_tag="IVK51_002247"
     CDS             10925..12076
                     /locus_tag="IVK51_002247"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP6431811.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4088 a         2130 c         2504 g         3390 t
ORIGIN      
        1 aatagtatac cgggggaaac tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt
       61 gattcctcat agattaagct ttaagcttgg actgtaagaa tctgatagaa aagcaggaga
      121 aaaagatgta tccacacacg aaacagctta ttttgaaaaa gatggaagaa ggattttatc
      181 caggtgtagc agtcgccttc ataaaagaaa atcaagtaga aacatttaca gccggctttg
      241 cccaagtcgt tcctgaaaag cagctgatga cagaagatct gttgtttgat gcagcctcac
      301 tgacgaaagt cgttgcaacc acttcattag ttttacgtct attagaagat ggaaagatag
      361 aactcgatca accgcttcat aaatatcttc ctgaatttga aaatcagaca atcactttgc
      421 gtcatttact gacacatact tctgatatca aaacatggat accaaacaga gataagctat
      481 ctcaggaaga attgatcgcc gcttatttga agctagaacc aggtgagcaa ctaggaaaag
      541 aagtaaaata tacagatgcc ggtacgattc ttcttgggtt gatgctagag gaaatttacc
      601 aagaaactgc agtggaaatc tttcaagaac aagtattgga accattagga atgatgaaca
      661 gcttatttct tccttttcct tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg
      721 aggttttgcg tggacaaact catgatccga aagcaagagt tttggctgaa catgcaggta
      781 acgcgggtct ctttattgat ttgaaagaca gcattacttt tgtccaaatg tatttgaaca
      841 agggaaaaat ccgtaatggt cgttttttag aagaagagac gattgattct ttactgcaag
      901 atcaaacacc gaaaaaaaat ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag
      961 aagacggtca tgcgattctt tttcatactg gttatacagg tacgtttttc ttgattgata
     1021 taaaaacaca agaagcgttg atatttttgt caaatcgcgt acatcctgtc gatcatcgtg
     1081 cggagtatct catatgtcga gatgaaattg ttgaaaactt tttgaaagag aaagcggtat
     1141 taccgaaata atgctataat agctatgaaa aatgagagga gtttgaatta tgcaggaacc
     1201 tatgttttta aaaccagttt ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt
     1261 ctttggtttt gatatcccta acgataaaat cggagaagat tgggcgatca gtgcgcatcc
     1321 tcatggtgtc agtgtagtag aaaatggcga attcaaaggg aaaagattag atgaactatg
     1381 gtcagaacac aaggaattgt ttggccatcc ttcagaacca gtgtttccat tattgatcaa
     1441 gatcttagac gcagaagatg atctatctgt acaagtacat ccagatgatg cctatgggat
     1501 gaaacatgaa ggtgaattag gaaaaactga atgctggtat atcatcgatg ctgaacctgg
     1561 tgcagaaatc atctatggtc atcatgcaaa gacaagagaa gaactagctg agatgatcaa
     1621 agatgggcgc tgggatgacc ttttgaagaa agtaccagtt aaaaaaggtg atttctttta
     1681 tgtaccaagc ggaacgatcc atgctattgg taaaggaatc atgattttag aaacacagca
     1741 aagttctgac acgacttacc gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac
     1801 aagagagctg catatccaac aatcgatcga tgtaacaact gtccctgcga aaacaccaga
     1861 attacaaatc aaagaagtcc gaaaagggaa ttcatcgatc gtcacttatt tagaaactga
     1921 gtttttcaat gtatatgagt gggatattaa aggaatcaca tctttcaaaa aacaagcccc
     1981 ttatacattg atgacggtaa tcgaaggagc aggagacctt gtcgttgacg gaaaaacata
     2041 tccattagaa aaaggaacaa gctgtattat tccaaatggc gtttctgagt gggcggtcca
     2101 aggtgaacta gcaatcattg cgactgaacc aggcaaatag ttcccagata gctgaaagca
     2161 gcagacggtt tttcgtttgc tgctttttct acataaaatc cgtccatcaa taaatagaag
     2221 tatggtgaaa aagatggaat acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa
     2281 acaactgaga aaagctggac atcacttaaa tgaaatgatc atgccaaatc taagtctgtt
     2341 tatcgcttgg ggaatcttgt cattagcaac aaaaggtgtg tctggcgatt tgcacaccac
     2401 attagaagag gtatgccatt ggatgatcca gctgctttta cccatcttga tcagttattc
     2461 aggcgggaaa tcggtagggg gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg
     2521 gttgatcgct cgttccaatg ctccacaaat cgtaggagcg atgattatcg ggccgcttgc
     2581 cggcatcgta tatgaacaat ttgtttctag attccacgtg aaattcaagg ctggatatga
     2641 aatgcttgtc agcaacctac tagtaggact gactggtagc gttatttgta taattggaat
     2701 cgtttttatt gaaccagttt taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg
     2761 gctagtcaca atgaatctgt taccacttgt cagtctgatc ttagaacctc taaaagtact
     2821 atttttcaat aatgcaatca atcatggtgt attgacacct ttaggaatcg aattctcaca
     2881 aacagtcggg tactcttcct tatttttgat ggaggcaaat cctggacctg gtttagggat
     2941 cttactttct atcctttgtt ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt
     3001 gatgatccat gcgattggcg ggattcacga aatctatttt ccatttgtct tgctgcgacc
     3061 gtatctattt ttagctgtaa tggctggtgg ggcaagcggc acgttgatat ttcaatggtt
     3121 agatacggga ttagctgcac ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc
     3181 gccttatcca caactagctg gggtaagtat agggatagca atcagtacat ttgtgacctt
     3241 catttgtgca gcgatactat tgaaattacc aaataaagaa aaagagaaca aaacggtcaa
     3301 agaaagggga gaaatcttgg aaccatctat cattcattcc gtcatttttg cttgcgactc
     3361 aggtatggga tcaagtgcta tgggagccgc cctgctgaaa aagaaattag cagcagaaaa
     3421 aatcgatctt tctgtcggat acagctctat ctatgaattg aataaccaag taaatcagct
     3481 ggttatcgtg caacatgaac tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat
     3541 ctatgtactg gatcactttt tggaaacaga tatcttgctg tcacgaataa aagaagcgat
     3601 ccaatttcca aagcgggaag aaaaaaaaga gatagcagat atcacagaac aaaaaaagtc
     3661 gcctgctaat attgtatttt tgtataaaaa caacagacgc ggctcacaaa ctatgggcat
     3721 gacgatttta agtcaattag caaaaaaagc gaaaaaacag tttgttatct caaaagaatc
     3781 aatagaaaaa atcaaaatcc aagaagaaac gatttatatc gcacaaagag aactagctgt
     3841 aactgaacag ctagaagaaa agatccctcg gctagttcta gttgaacatt gggtagctac
     3901 gaatgaatac gaaaaactcg tgaagggaga aatataagat gtatttatcg cccagagaaa
     3961 aacaattatt agcagaattt ctcagtagcc cgtcacctgt atctatccaa aaaatgatga
     4021 atttattgaa ggtcagtaaa cggactattt accgcgaatt agataatctt gaagtctcat
     4081 tgaaatcggt aaatgcttct ttgaaaaaag cagctagagg ctcatttgtc attgaggcat
     4141 cagaagaagt attagagcaa ttaaaagaaa atacagatgg gaaaagtaat gacttatcca
     4201 ctgctaaacg acaacacgcg attttagccg agttgttact gacgacagag ccgttgagtc
     4261 ttgctcattt catggaatca tatcgcatca gtaataccac tttttatgca gatatcaaac
     4321 agttggaaga aagtattcgc caactgcctt tggaaatcat tcggaaccag ggatacgaga
     4381 tcgaaggacc ggaaaaatac cgccggcttc tgacagcgaa tgtattagaa ttagaaatca
     4441 atgaatatga actttttcat agtatttctt tcgatgcatc actgaattat ttttttcgat
     4501 tcgttgatcc gcaacatcta tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa
     4561 aaaagaccaa tctaagtgat agaaagcttg aacatttggt tttgatgctg acattgacga
     4621 tggagcgtgt gactaaaaat catttactga caaatgagac atataatagt ctggcaaaca
     4681 aagaattact acagacagct aaacgattgt tttcacggat cgcttctgag acaaaacaac
     4741 tttatccagt aaatgagatc gtcttttttg caggattatt aaatgacttc acgaactcat
     4801 ttgatcagaa tttctttgaa gagacgttcg atacacagct tgcttattta gtaaaacaac
     4861 tgattcaaga agtaagtgaa gagacgaatg tccattttta tgaagatgaa actttgtaca
     4921 aaatgctgtt gactcattta tctggtgtgt tttcaagagc cgttttacaa gaagagacac
     4981 tgaccaatcc gattttagaa aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc
     5041 gtaaagcagt agagcggatt tttcaagaaa aaaaattatc cgaagaagag atcgcttata
     5101 tggtccttca ttttgcgaat tcattggaac gaagtccgaa agaaagcaat atcagtattg
     5161 caggattttc gccaagtgga ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt
     5221 attttccctt catccaacaa attcatttct atcggattgc tgatttaaaa aaaatcgatt
     5281 tacaagaaga atatgatgtt gttatttcta catctatttt accaggatac ggtggaaaat
     5341 atattttagt ctccccgcta ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt
     5401 cgcatattga gaagaaaaaa aggcaaaaaa ttacgtataa cagcaataac gagtggatga
     5461 tagaagatac ttatgaggaa acaatgcatt tcatggatca aatcaatcaa ttactcggtc
     5521 gtttttttat ccaagaactg gataatcctg agacgatttc cgaaacgatt cgcagcttat
     5581 ttccttattt tcctgaagga ctagtaacgg atgaagaggt ggttcatcgc cggctgctta
     5641 agcgatatga acaagcacct atcggtattc ctcatacaaa tatggggtta ttccatacat
     5701 ccagctcatt gatttctaaa ccgatctttt gtattttcaa tttgaaacat ccgttgacca
     5761 ttgaagggat ggatagacag ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt
     5821 cgccaatcca agaaaaagac gggattcttt taggaaagat cagtggagca atcatcatga
     5881 atgacttgaa caccgagatc ttccgctcag gaaataaaga aatcgtttat cagttgttag
     5941 cgaaaatcct gattgaagaa ataaagaaat gaaggtgaaa agatgataca gctaccagca
     6001 gaaaaaatat tgctcgatca atcctatcaa aattggaaag aagcgttgac cgagatcggc
     6061 cagtggatga cccaacaagg gggaatcaaa ccggcatata tccaatcaat gatcgaaaga
     6121 caagaaaaag cgagtgtcta tatcgggaat ttcgtagcct tgccacatgg gactgacaaa
     6181 gatgaagaag tagtagaaga aggaatctat ttagtccaag tgcctgatgg tgtggacttt
     6241 ggaacaaaag aggaaccaaa aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa
     6301 catcagttag cggtgctcca agatttagcc tttttctgtt cagacatcga ccaagtaatg
     6361 gcgctcagtg atgcgaaaac cattgaagaa gtccaaatga tttttgaaca tggacatata
     6421 taaccaatgt tcacagtaaa aaaagctgcc ttgcatcaag taataacttc aaccaagata
     6481 aaaataagag gagctatttt tgttgggagg ttcttattac tgcggatgca agcttttgaa
     6541 aactatttat tctagaaaac cgtttatttg aaatagggga cggtgacaag gtttttgtca
     6601 ccgcccctat tatttttgtg ttttaatagc tcttttttat atagatagtg cccataccta
     6661 tgaataataa aacgatactc catataccaa gtgtcaagat accattcatg aaagtaacct
     6721 gttctgaagc tagtggcgag aaaatatctc ctcctatgat cacattttga aaatcaacat
     6781 aagaagttgg caagtaatga ctgattgatt ctatccatcc aatcgaagaa aacaagcgat
     6841 actctgctaa gaaaagacaa agaagtgtac ctaaaagatt gactagagaa tgttcaaata
     6901 aagcagatag aagaaaactg attgtactta aaaagagcac ccacaacaac aggaacagaa
     6961 agctctggaa aagatactca tacatcggaa ggattcgtat ctcctgacca atgatcgttc
     7021 ctacaggata acggaagtct cccaaaccat ttttgattat tactagtata gagacgatca
     7081 aaaatggcat cacgagactt agcatagctg caccataata aatggtaaaa cggatgctat
     7141 gtttctttac catactatcc ggtaatagat tgactagaga gattacctgc tttctttttt
     7201 cataagagat gatggaagaa agaaacaagg cggttataca taaaatcaaa aaactagaaa
     7261 tcattcctga aaaaatattt tcgtagtaat ttgctaaagg gagatgcgct ggcaagtcaa
     7321 aaatagaata tctttggatc tgatgttctt tcatataacg gagcaactcg acacgttttt
     7381 gttgttcaat gattggaatt ccttgtcttt gaccagaaat cagtcgatcc agtatatctt
     7441 tttcataatg ataagttgct tcgaccattt gctggacatt ccctgtgttt tttgcatgta
     7501 agattgcttc gatcagtttg ttgcttttct ctaaactttc cacaaattct ttctcattct
     7561 catcttcttt caacgtttca atcaaacgtt gcatctcttc atagttatga ttcagttcat
     7621 cgaattctgt ataagcaagt ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca
     7681 acaatagagg aaataaaaag tttactttgt tttttagtaa gagattgcgt tcaaatgtaa
     7741 gtaaagacat agttacacct catataattt acgataaata gctaaaatct cttccatatt
     7801 tgaggattct gtgacgattt ctccattttt tagaaaacat gctttagaac aaatcgattg
     7861 gatattcgcg atagcgtggg aagaaagaag aatcgtcttc ccctctttct gccattgtct
     7921 gatgaggcta ttcaagatat caatacttcc tgggtctaat ccgttcaacg gttcatcgaa
     7981 taacatgaca gatgaatcac tgatcaagta agcagcaagc aagacatgtt gtttcattcc
     8041 tagagacatt tttccaatcg gcaaagtaag gtactcagtc atcttcaatc gttggaaaac
     8101 agtttggcat tctactttgc tctgccaata gtatttgatc aaagtcaaat aatcaacagc
     8161 cgatagatag tcggataact gattcgttga ttctagaaaa aagaaagacc ggcgataatc
     8221 tgtataagca gtaggagaga tgttgtgaag ggacaattga tcataagttg ttggcagcag
     8281 ttgggcaatg ccattcagca aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc
     8341 tacttctcct gattctacta aaagattcaa ttgtcgaatc acacgttttt taccacgttt
     8401 catgttcatg tttttgatca gtaatgtcat agataggcct ccaatataaa atttaataac
     8461 ataatagaat ggaaaacatc taaattaaat gctaacaaaa gttattgttt gcatattata
     8521 aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt ctgataccga aatcttttta
     8581 ttttgctgac atactcaaac ctttggcaca aagtttcgtg ccaaagtacg actatttctc
     8641 tttttagtaa gcggatacaa ttagagtatc aaaaggagag agggagagac aaaatggaac
     8701 ggacgattcc caaaaacaac atttcgctaa aagcgagggt acaaaaatta ggcagtactt
     8761 tatcaagcat ggtcatgcca aatattggag cactgattgc ctggggtgta ttaacggctc
     8821 tatttatccc agatggttat ttaccaaatg aggctttggc taccatggta agcccaatgc
     8881 taacttatct gattccttta ttgatcggtt atactggcgg aaaagtaatt gctggtgacc
     8941 gcggatcagt tgttggggca attgcaacga tgggtgtgat tgtcggaaca gatattccaa
     9001 tgatgctagg cgcgatgatc atgggacctc ttggtggata tgccatcaag aaatttgatc
     9061 agctattcca aaaacggatc aaatccggtt ttgaaatgct agtcaataat ttttcagcag
     9121 gtcttatcgg ttttggatta gcgttattag gtttttctgc gattggtcca gttgtcgatg
     9181 cattgactca agcaatggca aaaggtgtag agatcatttt atctgcccat ttgattccat
     9241 tgacaagtat ttttattgaa ccagcaaaaa tcttattttt gaataatgca atcaatcatg
     9301 ggattttgac accattggga actgaacaag tagcagaggc tggaaaatct attctattct
     9361 tgcttgaagc aaatcctggt ccaggacttg gcgtattact tgcttttact ttgtttggca
     9421 aaggagcagc aaaatcttca gcaccaggag ccatgatcat tcacttttta ggcgggatcc
     9481 atgaaatcta ttttccttac gttatgatga aaccgttatt attcttatca gttataggcg
     9541 gaggaatgtc aggaagtttc attttccaat tgatggatgc tggactaaga gcgccagctt
     9601 ctccagggtc gatcattgcg attctagcga tgacaccgtt caattcttac cttcctgtta
     9661 ttttgggcgt gattgttgcc gcagctgttt cgtttggtat ttctgcagta attttgaaag
     9721 cggatgcgaa agatacaaat gaagacttcg aaaaatctgt tcaagagaca aaggcagcta
     9781 agcaagcttc caaaggtgcc caaggtattc ctcaaggagc tacattctct ggcgtacaaa
     9841 aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga
     9901 gaaaaaaagt acaggaagca gacttgcctc aaacggtgac caatcaagca atcagccaac
     9961 tgacagatga ctcgaataca ttgatcgtta ctcaggtcga actacaagaa agagcaaaac
    10021 aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt tttgaattca cctcgatatg
    10081 atgaaatcat tcaagaatta agcagcaagc agaaaacatc agtttctgaa attgagaatg
    10141 agccagcaga agagtttctt tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc
    10201 acgatgatcg ttccggatca gcaacaatgg ggatgaccgt gctcaaagat atattagaca
    10261 agaaccattt aactatcccg ttaagaaaag tcaatatcca agatttaaaa gatgaacctg
    10321 attcactgat cgtagccaaa gaaagtttag cggaaaaagt acagatggct gcacctcaat
    10381 ccattcattt atcagtcaac agcatggtca atccgcaaaa atacgaatcg attgcacaat
    10441 atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa agtttgccat tcgacatgat
    10501 cgagctcaat cgatctttta aaaccaaaga agaagcgatc cgctattgcg gtcagatgct
    10561 tgtagaagca gggtgcgttg atgaggaata tatcgatgcc atggtagata gagacaaaat
    10621 gttatctgtt tatatgggga attttatcgc gattcctcat ggaacagatg aagcaaaaaa
    10681 atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca gatggtgtag attttggtac
    10741 cgaagaagaa gaaaagattg cgactgtttt gttcggtatt gcaggagtcg gagatgagca
    10801 tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat atggacaatg ttgtccagct
    10861 agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta gcaattgctt aaggagagag
    10921 aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt cgaggattta ttggagaggt
    10981 tttggcagct aatggatttg agattacttt cgtagatgtg aatcacacga tcattgatgc
    11041 tttaaatgaa agaaaagaat acgaaatcga attagcagat gaatcaaaag aaaaaatcac
    11101 tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat
    11161 cgctgaagca gatttagtta ctaccgccat tggcccgaat atcttacctt ttattgctga
    11221 gttgattgca aaaggtatcc aagaaagaga aaaggaagga agcacaacat ctttagatat
    11281 cattgcctgt gaaaatatga ttggcggttc agcatttcta gaaaaagaag tgtataaata
    11341 tcttccggaa acatcattta ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg
    11401 aatcgtccca ttacaacacc atgaagatcc tttattcgtc caagtggaac ctttcaaaga
    11461 atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc caattaaaag gggttttata
    11521 tgtagatgat ctagaaccat atatcgaacg caaactattt agtgtcaata ctggtcatgc
    11581 gactgtcgct tatacaggtg ccttgttagg ttaccagacc attgatgaag caatgcaaga
    11641 tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa acaggaagtt tgctgattgc
    11701 caaatggggg tttgatgcag agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt
    11761 ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct cggacaccat tacgtaagtt
    11821 aggttatcaa gaaagattta cacgtccagt gagagaacta caagaacata acttgacttg
    11881 cccacatttg acggcaacaa tggggattat ttttaattac tatgatcctg aagatgaaca
    11941 aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg gaacaattga ttcaagaagt
    12001 cacaggaatc aatgatccga aaacaatcgg gaatatcaag caaaacgtga accgttatgc
    12061 caagcaagtc gcttaaaata ggatacgaaa aaggggaatg tgacgaaatt tt
//
LOCUS       DACCDF010000080        12065 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCDF010000080 DACCDF010000000
VERSION     DACCDF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.083"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            39..2057
                     /locus_tag="IVK51_002248"
     CDS             39..2057
                     /locus_tag="IVK51_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6431812.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2484..4898
                     /locus_tag="IVK51_002249"
     CDS             2484..4898
                     /locus_tag="IVK51_002249"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP6431813.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5246..6799
                     /locus_tag="IVK51_002250"
     CDS             5246..6799
                     /locus_tag="IVK51_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP6431814.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7180..8964)
                     /locus_tag="IVK51_002251"
     CDS             complement(7180..8964)
                     /locus_tag="IVK51_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431815.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8979..10700)
                     /locus_tag="IVK51_002252"
     CDS             complement(8979..10700)
                     /locus_tag="IVK51_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431816.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10800..11561
                     /locus_tag="IVK51_002253"
     CDS             10800..11561
                     /locus_tag="IVK51_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP6431817.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3956 a         2177 c         2439 g         3493 t
ORIGIN      
        1 ttattaataa tagataaatt gaagttggag gatagaagat ggaaaacatt gcaagaaaag
       61 aacggagacg tttagaacag accaaacgtt ttcgaaaagc aaaacgaggg gcagcaatcg
      121 tgggaactgc gatggtaggc tgttctgtcg ctgccccatt agtacagccg gttcctgttc
      181 aagcggatga aacacctgtt cagttcggcg caagaatcaa tacagcggca tttatcacag
      241 aaattgctac ttatgctcaa ccaatcgctc aagccaacga tctatatgca tcagtgatga
      301 tcgcacaagc agttgtagaa agcggttggg gcagtagtgc gttatctcaa gcaccttatt
      361 ataatctttt tgggataaaa ggcagctatc aaggacaaac cgtctacatg gatacgcttg
      421 aatttttaaa taatcaatgg gtaaataaaa aggagccttt ccgacaatat ccttcttttg
      481 ctgaatcttt caatgacaat gcctatgtac ttagaaatac ttcatttggc aacggttatt
      541 actatgccgg tacttggaag agcaacacga aatcctatac agatgcgaca gcttgtttga
      601 cagggcgtta tgcgactgat cctggttacg caggcaaatt aaataatatc atcgcaactt
      661 acggattgac ccaatatgat acaccagcta gcggaaacgc aggcggtgga gcgactacag
      721 gtggcaataa tactggaagt gcaggtagta ctggcggttc aagcaattct ggtaatacaa
      781 actcaggttc agcaacttcg accacttata ctgtccaatc tggtgattct gtttggggaa
      841 tctcaaataa attcggtatc acaatggatc aattgatcca atggaacaac atccaaaaca
      901 atttcattta tccaggacaa aaactgacaa tcaaaggtag ccaggaaaat ggctcttcga
      961 cgaataattc cggtaacaat accaactctt caggaaatgc tggtacatca aacggaggac
     1021 aaacaactgg tgcgaaatac actgttcaat ctggtgattc tgtttggaag atctcaaatg
     1081 atcacggaat tacgatggct caattgattg aatggaacaa tatcaagaac aatttcgttt
     1141 atccaggaca acagctaatc gtatcaaatg gcggttcttc taatgctgga gcaaatactg
     1201 gctcaaatac tgggaataca agtggctcta gttcgtccag ctcaagttct tctaatacag
     1261 cttcaggaac gaaatataca gttaaagccg gagactctgt ttggagcgtc tcaaataaat
     1321 acggaattac gatggctcag ctaatccaat ggaacaatat ccaaaataac tttatctacc
     1381 caggacaaca actgactgtt tcaaacagtg gatcaacaaa cagcacgtca ggtaatacag
     1441 gttcgtcaac caatacaaat aacagcagtt cagttaatac tggcagcagt tcgtctcaag
     1501 gcacttatac cgtgaaagca ggtgaatctg tttggagcgt ggcaaataaa aacggcatca
     1561 cgatggatca gttaatcgaa tggaataata tcaaaaacaa cttcatttat ccaggacagc
     1621 aattgatcgt taaaggtgga acttcggcaa atacaaatac aggttcaact acttctgcag
     1681 cgaaaccaaa tacaccgaat acgtctgcta cgacaagtac atcatccact ggtaatacga
     1741 tgtataccgt gaaagcgggc gagtcagttt ggagcgtagc gaacaaacat catatcacga
     1801 tggatcagtt aatcgaatgg aataatatca aaaataattt catctatcca ggacagcaag
     1861 tgattgtgaa gaaaggttca tctcaatcaa caaaccaaca atcagttact ggttcgaaaa
     1921 cctatactgt gaaatctgga gaatctgttt ggagtgttgc tgacagtcat ggaattacaa
     1981 tggctcaact aatcgaatgg aacaatatta agaacaactt tatttatcca ggtcaaacgt
     2041 tgattgtagc taaataaaat agctgttcga agaaataacg attgaattcc acaaaaatat
     2101 ttgttactat aaggctactt aaatatgaca acacttgaaa aggaagtagt aaaaagactg
     2161 aagtttcaga gagcgcatgg ctggtgagaa tgtgcacgga tgcttttgaa ctcgcctttg
     2221 agttgccctt gctgaatacc cgagtaagca agcggcggtg acggccgtta caacgttcga
     2281 ggccttttca ctaaggtaaa attaggtggt accgcgtaga ttacgtccta taaaaagagc
     2341 aatcttttta taggattttt ttatttcttt tgtgaagttt gagaaaacaa agcaaaagga
     2401 aataaaaata aacagattct gccggttttt tcgttatatt tataatagaa acaagtgttt
     2461 tcaatagaac aggaggacag caaatgagtt acaatcataa agaaattgag aaaaaatggc
     2521 aaaaatactg ggcaaaaacg aatactttca acacccatga tgatcctgaa aagccaaaat
     2581 tttacgcact ggatatgttt ccctatccat ccggtcaagg gctacatgta gggcatcctg
     2641 aaggttatac ggcaacggat atcctttcac gtgtgaaaag aagtcaagga tacaacgtgc
     2701 tgcatccaat ggggtgggat gcattcggtc tgcctgctga acaatatgcg ctagatacag
     2761 gaaacgatcc agctgagttt acccaaaaaa atatcgaaac attccgacgc caaatcaatt
     2821 ctttaggatt tagttatgac tggaatcgcg aagtgaatac aactgatccg gagtattata
     2881 aatggacaca atggatcttt acgaagcttt atgaaaaagg tctagcttat gaagcagaag
     2941 tagcggtaaa ctgggtacct gaattaggaa cagttatttc aaatgaagaa gtgatcgatg
     3001 ggaaaagtga acgtggtggc tatgatgtag tcagaaaacc aatgcgccaa tggatgctga
     3061 aaatcactgc ttatgctgat cgcttattgg atgatctaga attagttgat tggccagaaa
     3121 gcattaaaga aatgcaaaga aattggatcg gtcgttcagt aggagcaaat attgaattta
     3181 aagtggctgg tacggataaa tcctataccg tatttacgac acgaccagat actctattcg
     3241 gtgcaactta ctcggttctt gcacctgaac tagatcttgt tagagaaatt acgacccctg
     3301 aacaaaaagc tgctgtagaa gcttatatcg aagagacagc taaaaaatca gatctaaaga
     3361 gaacggatct tgcaaaagaa aaaacaggag tatttactgg tgcttatgca atcaatccag
     3421 ttaatggcaa ggaaatccca atctggatcg ccgattatgt attatcttca tacggcacag
     3481 gagccatcat ggctgttcca gcacatgatg aacgtgactt tgaattcgct caaactttcg
     3541 gactagaaat tcttccagtt atcgaaggtg gagatgttga aaaagctgct tacacagaag
     3601 atggtacgca catcaactct gaatttctag acggcatgaa caaacaagaa gcaatcgata
     3661 aaatgaatga atggctagaa gaaaatggcg tagggaaaaa agaagtcagc tatcgcttac
     3721 gtgactggct attctcccgt caacgttact ggggtgaacc aatcccaatc atccattggg
     3781 aggacggaac agtcacagct gtcccagaag aagagctacc attacggttg cctaagacaa
     3841 aaaacatcaa gccaagcggt actggagaat ctccattagc aaatatcgag gagtgggtca
     3901 atgtcgttga tcctgtaaca ggtaaaaaag gccgtcgcga aacgaataca atgccacaat
     3961 gggcaggaag ctcatggtat tacctgcgtt atatcgaccc tcataacaaa aaagaactag
     4021 cagattatga aaagttgaag cgttggctac cagttgacat ctacattggt ggagcagaac
     4081 atgcggtact tcatttacta tatgcacgat tctggcataa attcttatac gatatcggtg
     4141 ttgttcctac aaaagaacca ttccaaaaac tatacaatca aggaatgatc ttaggcgaaa
     4201 acaacgaaaa aatgtcaaaa tctcgtggta atgtagtcaa tcctgatgat gtggtcgaaa
     4261 cttacggtgc agatacactg cgcatgtacg aaatgttcat ggggccatta gatgcttcga
     4321 ttgcttggag cgaaaatggc ttagaaggaa gccgtaaatt cttggatcgt gtatggcgtt
     4381 tgatcgttga tgagaacaat aaaatgcgtg accgtatcac tacattgaat gatggcaagc
     4441 ttgataaagt gtatcatcaa acagtgaaaa aagtgacaga agactatgaa aatcttcatt
     4501 tcaatacagc aatctcccaa ttgatggtct ttatcaatga agcctataaa gtggacgctc
     4561 tgccttatga atatatcgaa ggatttgttc aactactcgc accgattgct cctcacatgg
     4621 gtgaagaact atgggcaatc cttggaaatg atggtggtat ttcttacgca ccatggccaa
     4681 catacgatga agcagcactt gttgaagacg aagtagaagt tgttttccaa gtaaacggta
     4741 aagttcgtgc gaaaagcaat gttccccgag acttaggaaa agatgagcta gaaaaagcag
     4801 cattagccaa tgaaatagtg caagaatata ttgaaggcaa aacagtcaga aaagttattg
     4861 cggtaccaaa caagttagtc aatatcgttg ctaattaatt ggctctttgt caataaggac
     4921 tgatgagttg tgcaaaatca aatctgggcc agaatgaact tcattctgtc tcagattttt
     4981 tgttatttca aattgattaa ttgattgatc aaaaaggttt tgattttcat gttctcaaac
     5041 acgttcaatg tctttttctt ctgaatggtc ttgtaagaga tctctgtaat catcaaattt
     5101 ttgtctatta atcttagtat tttttgatgt ataaactacc tgaaattgag agtacatacc
     5161 aaattcataa attttttatt agtgggacta gtgattgtat ggattttgac gcataatgaa
     5221 tgaagtatga gaaaaggaga gacaaatgga tcaagaacta tttattatcg aagtattatc
     5281 agctaaacaa aaaattatcg agaaagcgaa aaaaagagcg aaaagaataa tgttcaagct
     5341 tttaagctta gtatttttct ctggtttgct ttttttcgga gctgtatcgt tcctatatcg
     5401 ccatatgagt gcagtagtcc gttcagctga agggatgcaa aaattaaata atttgtatca
     5461 cttttcgtgg ggagttggcg gaataacaat tgttgtgctt attgtttttc ttctacttat
     5521 ttggctccgt taccatcaat tgtcgcgtca tttcgatcaa tggcttagtc aaagagcaca
     5581 acagttttta cataataaac caacttctca agatgatgac tatctttatt ttgagaagac
     5641 ttcgacgagt caagcttttc ggatacaaaa aagtgattgc cagttattgc tgagatcact
     5701 tgatgattta cctatctact taggcgaaag caaatcaaaa ttcggaatga acacattcca
     5761 tattttttta ctagaaaacg aacccagatg ggaaaaaagc cagtcttcgc ctggtggata
     5821 cctgacaact aagatgagta cattagtcac tggttttttc ctcattcttt tagctggaac
     5881 aatctattat gggagataca ctcagccaaa aatgtcttct catataaatg ctgcttctca
     5941 catgacaatg aactcaacta acgatacgca tatacaaaag acaaatgaag ggttgttagc
     6001 tgatcctaag caaaaggtaa atttgacaac acaaaagata aatgatctca aacttgatac
     6061 agagacgcac tctctctata tgacaacaga tagtggtgct aattgggtat ttgtgccgat
     6121 tgattcgcaa tggttgcgtt ttggcgaata caccctgaca acggggctga ttcctgaagg
     6181 ttattggatg gacaacacat atgatatttc cgccgatttc tcatggttta tctattcttc
     6241 tggggatgat gtacgtctgc tgacttcaag agataatggt aaaacatggc aaacgtctat
     6301 tatcacccaa tcagttcagc cgatacgtta tcgaaaagcg gtattttatg gcaacgatgg
     6361 tctatttatc tgtacatcag ccagtgggat gtctgcagaa actttgtaca tttacactac
     6421 tatggatggc ggaatcactt ggataaaaag tggaagtaca atgatcaatc agccagtaca
     6481 aaatgtcagc ttcgttacaa cttctttagg ttttgtttcg acacgtgaaa gtttgtttta
     6541 tacaaacaac ggaggacgct cattcaaaga atcagttctg tcgattccgg aaaactatca
     6601 gacagggggg atagatcttt ttcaatcacc aaatgaagtt actcaaataa gcgcgaatac
     6661 gttagagaca aaattttatt tgttgaaaaa aggaccaatc gatgcaggga aaatgtttgc
     6721 ctgcctcttc cgttcaactg ataacgggga aacatggcaa ttcgttcagc aactatcgca
     6781 agtagaacaa ggagattaaa gaagagagat tgtgagcctg acgttcagca ccgagtcaca
     6841 agaaacaaat tcaaaaaaca gcttctcata tttgttgaat agtgtaactc gtgattcgga
     6901 gttgacaaga gcaacaagaa gcgattttga agtatagctt ctcgtatttt acaagatcgt
     6961 gtcttattgc cgataggttg tctcttgaac accgtttatt cgtaaaaagg gattgtgaca
     7021 agtcttttgt cactacttac cgaacgaaac tgaaggagct atccaaaaag tatcattagt
     7081 attattcttt gtgtttctag tggcaccact agtacattta aaaaagctat cgatcatgga
     7141 ctaatcatac atgatcgata gctttttaga taatgtttgt tacgctatct gaaattgact
     7201 attataaaga ctggcataaa gcgttggtga ttggagtagt tcagaatgtg ttcctttttc
     7261 gacgatatcg ccattcttca ttacaaggat catgtctgca ttttcaatcg ttgacaaacg
     7321 gtgtgcaatc acaaaacttg tcctatcttt tgtcacagct tccattgctt gctgaatcaa
     7381 tgcttctgtt cgtgtatcta cgctagaagt tgcttcatct aaaataacga caggaggatt
     7441 cgctagaatg actcgagcaa ttgtcaataa ttgttgctgt ccttgagata acgtgccgtt
     7501 ttcgctagaa atgatcgtat catatccgtc tggcagtgtt cgaataaagt gatcacattg
     7561 tgcgatttca gccgcttcga taatttgttc tcttgtggca tccattttcc cgtaggcgat
     7621 attatcagct accgttcctt caaacagcca ggtattttgt aaaaccatcc caaaagaacg
     7681 acgtagttct ccacgtgaaa gcttggtaat atcaagaccg tcgaattcga tctttccttt
     7741 attcagttca taaaaacgca tcagcagatt gactaaagtc gtttttcctg caccagttgg
     7801 accaacgatc gctaccgttt gttttggctt tactgaaaaa tcgacttgat tcatcaatat
     7861 tttatctgat gtgtaaccaa actgtacatt tttgaaagct atttttcctt tcggttgttc
     7921 taaaagtctt gcatctggaa ggtctggaat ttcatctgtt tcatctaata tctcaaatat
     7981 cctatcgatt gctgccattg cggattggat cgagttgatc acataagaag cggtagaaat
     8041 tggttcagaa atctgattga tgtactgaag ataagcttgt aagaagccga tcgtcacacc
     8101 acctgataga acaagcatag caccgaatac cgcacttatg atgaaggcga gttgattaat
     8161 gaaacgaatg gccggataga tagcaaagtt cataaattga gcacgtttaa aagcgttgta
     8221 ttgcttttta tttacttcgt gtatagcagc ttctgcatga gcttgctgat tgaatgtttt
     8281 gaccactaga ttaccagcta agatttcttc cattcgatta tttaaaacac ccagttcata
     8341 ctgattctgt tcagaaatct gtttgttttt gttcgcaatc ttagttgtaa caattgaact
     8401 gccaacgatc agcaataaga cgagcaaagt aagcttgctt tcgatataaa acagcatcag
     8461 accagcagca gctattgtta cgacagaagt aaaaaattga ttgatacctg tcaataacac
     8521 ttgagaaagt tgattgagac cggtagttgt ccgactaatg atgtcaccta cttgatgatt
     8581 gtcaaaaaac gacatcggta atgttttgaa tttttttgtg atttcttttc gcaccctcaa
     8641 tgtgatcttt tcgcttaacg ctgccattgt ccgttcctga ataaatgaag tgatgctgct
     8701 taagactgaa cagccgatca gcaataagac agggaacaac aatgcttgtt tgatatcact
     8761 gaaagaaagc tgtccaatac ctgtttttct tatttctgat aagagatcat cgatcccgat
     8821 tcccatgatc aagggcatgg ctacgactaa taggttcccg aataaactgc atagaaacaa
     8881 tccgtaaaaa tatttatgtt caggtttgat cattttccaa aaacgtatca aagcacgcgt
     8941 gttcaacgtt tttttctttt ccatcgtatc ccttctttct atattccttg tgattttgca
     9001 aactcctgat aagtttggca attagacagt agttcttcat gagtaccttg tccaacgatt
     9061 tttccttcat ctaaaacaat gatgttatct gcttgttgga ttgtactgag gcgttgagca
     9121 acaatcacga gagttttctc cgacatgtgt ttatgaaggg ctgctcttaa tgctgcatct
     9181 gttttatagt ctaaagcgga gaaactatca tcgaatacat agatctctgc tggtttgatc
     9241 aacgctcgag caatacatat ccgctgtttc tggccccctg aataattaga accgccttga
     9301 gcaacaaagc tgtccaatcc gtcaggaagg gtgtccacta gttcttctaa ttgagcgatc
     9361 ttgatcgctt tagacaactc ttctgttgag gcatattcat ttcccatctg caagttgctt
     9421 aaaattgtcc cgctgaataa gaaagccttc tgagggacat aactgatgtg ggagcggatc
     9481 tgttcttgag tcatttgagt aagaggaata ccaccaaggg ttacttttcc tatcgtaggg
     9541 tcactaagtc tcaagagaag tttcgccaaa ctactttttc cagatccggt cccgccaacg
     9601 atcgctgttg tttttccttt aggtattgtg aaatcaatat cttctagaac tggatcagag
     9661 atttcagtat atttaaatgt gacatgttca aaagcagctg catttgtctg atcaaaggtt
     9721 ggttcagcta ctgttttgat cgtctcttcc tcaatttcga tctctgcttg cagtatttct
     9781 tctatccgtc gtaatgaggc aaacgattta gggataatga ccaataccat agctgcgatc
     9841 atcaagtaag caagtgtcag catagagtat tcgactacag cagtcacgac accgatttgt
     9901 aatgctcctt ctgctacaag atttccgcca aaccacaaaa tagaagcata aacgattccc
     9961 atgagcaaaa aagcaattgg agtgacccaa gcaaatattc ggttcacttt gatcatttga
    10021 ttagcatagt cagcaaagct cttattcgtt cgcttttctt catatgattg attatcaaag
    10081 gcacggatca tattaatccc agtaaagaat tctctcaatg tgatggtgat ccgatccatt
    10141 tttggctgga tactcaaaga taaaggtgcc cctcgtttca tcagtcgata aacaatccat
    10201 acataaaaaa caacagagat caatgtaacc catgttagta atagagaata acgaaacatc
    10261 atgagaatgg tgaaaacgca aataaccggt gctggtaaga tcatttggca aaataaaaca
    10321 atcatttgct ggacattatc gacatcattc gtcattcttg tcaaaagaga tccagaacta
    10381 aaacgactga tatccttcat tgacagctgt tggaatttac ggaaatattt ctcccgcgtt
    10441 tggaatccaa acctagcgga agccaatgct gaaaagtaac tagaggcaac tgcagcgatt
    10501 gttcctagaa acgctacaag cagcatttca actccaattt tttttataac tgaggcttcg
    10561 ccacttgcta tacctgtgtc gatcagcttt gctactaatt ttggtacacc taatgtaccg
    10621 gcgatttgta aacagatcat cacaaaggta aggagagcga gtcctttgtt tttctgtaag
    10681 aatgttttta agatagacat attattcctc cttttttcct gatacttttc gattctatac
    10741 cttcaagtaa cttgaaggtc aatagaaaaa agaataaact gaaaaagagg tgagaaaaaa
    10801 tgaaaaaaat gtatacgatt ggccaagtat cggaactatt tgatttgcca aaatcaacga
    10861 tccgctattg ggatgaacaa ggattaatcc gttcaagtag acaagaagag aatgattatc
    10921 gattgtttga tatcgatgat atttttatgc tttatgatat cgttttttat agaaaattag
    10981 atataccgat caaacagatg aagaatttat atggaaaaac attgacggag ttatatgaga
    11041 cacttgatga aacagaaaga agaatccata aagaactggt ggtgatgaaa caaaaacaaa
    11101 aagagataag ggagcaaaaa aaacagttga aattgatgat cgatacaaac gaggaagaat
    11161 ttcctgtaga agagatccct tttgactgta tgatctcaac ggagtttgaa gatattgtgg
    11221 aaatcaaaaa atttctacct aactattcaa gctttgggat gatgagtatg ccagcttcca
    11281 gttcacagac gatgtatgga ttttttatcg accctagtga agtacattta tttactcaag
    11341 acgtgatttg ggagaaaaaa gatacggctg tttatcgtcg attcttattg aaatccgaga
    11401 tgaaccatgc ggaacgtaat aatattttag aaatcagaga aagaatgtat gaaaaaggat
    11461 ggaaaacagg agaagtgatc ggccagtatc tattgacgaa tacggatgaa aacaatatca
    11521 gaactgaata ttatcatgca tggatagaaa tgaaaaaata ggaaatatgt gtttaagata
    11581 actcgaaaat gatggttgtt ttagttataa agaaaaattt tctggttcga ttacgggttt
    11641 aatgtggggg attattagtc gtggatagat agaattttag ttttttagag gaaatatgtc
    11701 tttaaaaatg gaatggttgc aattcttagc gatatatacg atgtaattgt cctcttaaaa
    11761 aagtatggaa aaataattgt attgggtgtt atgtataatt catggtgaac tgagactaat
    11821 gagaagaaaa attcatattg ttatgagtga gattgtatca gatgagaatg ataaattaaa
    11881 taggtgcata aaagcattac acttaaaata cattgttgct tacattatta caatatgtta
    11941 ataaggcaaa aataaataga tggattgaat aaacatattt tttaatcaaa tagtccctac
    12001 tgtgactttc ctcagctatc ggtgatacta ccgatagcat ttttttgctt ataaaataat
    12061 tataa
//
LOCUS       DACCDF010000081        12060 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCDF010000081 DACCDF010000000
VERSION     DACCDF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.084"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..197)
                     /locus_tag="IVK51_002254"
     CDS             complement(<1..197)
                     /locus_tag="IVK51_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP6431818.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEE"
     gene            complement(204..434)
                     /locus_tag="IVK51_002255"
     CDS             complement(204..434)
                     /locus_tag="IVK51_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431819.1"
                     /translation="MKYEIPLSEAGIQAIINGREVNIELPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDNTFRLGS"
     gene            complement(431..742)
                     /locus_tag="IVK51_002256"
     CDS             complement(431..742)
                     /locus_tag="IVK51_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431820.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDV
                     K"
     gene            complement(881..1696)
                     /locus_tag="IVK51_002257"
     CDS             complement(881..1696)
                     /locus_tag="IVK51_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP6431821.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            complement(1693..2019)
                     /locus_tag="IVK51_002258"
     CDS             complement(1693..2019)
                     /locus_tag="IVK51_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431822.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            complement(2016..2177)
                     /locus_tag="IVK51_002259"
     CDS             complement(2016..2177)
                     /locus_tag="IVK51_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6431823.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            complement(2192..2551)
                     /locus_tag="IVK51_002260"
     CDS             complement(2192..2551)
                     /locus_tag="IVK51_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP6431824.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(2548..3399)
                     /locus_tag="IVK51_002261"
     CDS             complement(2548..3399)
                     /locus_tag="IVK51_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6431825.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGSIA"
     gene            complement(3414..4214)
                     /locus_tag="IVK51_002262"
     CDS             complement(3414..4214)
                     /locus_tag="IVK51_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6431826.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            complement(4257..5147)
                     /locus_tag="IVK51_002263"
     CDS             complement(4257..5147)
                     /locus_tag="IVK51_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP6431827.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            complement(5149..6090)
                     /locus_tag="IVK51_002264"
     CDS             complement(5149..6090)
                     /locus_tag="IVK51_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP6431828.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            complement(6323..6658)
                     /locus_tag="IVK51_002265"
     CDS             complement(6323..6658)
                     /locus_tag="IVK51_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431829.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            6925..7149
                     /locus_tag="IVK51_002266"
     CDS             6925..7149
                     /locus_tag="IVK51_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6431830.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            complement(7458..7613)
                     /locus_tag="IVK51_002267"
     CDS             complement(7458..7613)
                     /locus_tag="IVK51_002267"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431831.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            7685..7915
                     /locus_tag="IVK51_002268"
     CDS             7685..7915
                     /locus_tag="IVK51_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP6431832.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            complement(8083..8349)
                     /locus_tag="IVK51_002269"
     CDS             complement(8083..8349)
                     /locus_tag="IVK51_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6431833.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            8549..9253
                     /locus_tag="IVK51_002270"
     CDS             8549..9253
                     /locus_tag="IVK51_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAP6431834.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            9340..10629
                     /locus_tag="IVK51_002271"
     CDS             9340..10629
                     /locus_tag="IVK51_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6431835.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            10695..12047
                     /locus_tag="IVK51_002272"
     CDS             10695..12047
                     /locus_tag="IVK51_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6431836.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
BASE COUNT         3634 a         2343 c         1960 g         4123 t
ORIGIN      
        1 atttcctccc atttacgatc atcatttaat atcgaaatcc caaacttacg aatagcatca
       61 cttgcatcag caacaaactg acttgccact ttatatgttt cttctgctga aattccatat
      121 tctttttcaa actttgtctt tagtacattt agttcctgtt ttcttagttt tgctactctg
      181 cggtgtctgg tgttcattgt caatcaactc cctaatctga aagtgttgtc tatacttgat
      241 cgaaattctt ttaagtggtt ctctaccaca gaatcagtca cgttaaaacg atcaattaat
      301 actggagctg ccatatcttt caaataactt tgtctgatga ctaattcagt accatcagga
      361 agttctatgt taacctcccg accattgata attgcttgaa tgcccgcttc acttagtggt
      421 atttcgtatt tcatttcaca tcctccaaat cactcgactt cacgaataca ccatctacca
      481 ttttccctgt gcgtcctttg atttcgttgt atgccatttc taaacactct tgtacgtttg
      541 tccctttttg catggaaagg ataatcagcg tgacgataac gtctcctacg ctatctttaa
      601 atagttcatc attacttctt gccatcgccg aagctatttc cccgaattcc tcagctactt
      661 tcaaaaactg tgcttttgga tctgcttgat ccagtccctt gtctttagcc cactgctcta
      721 cttttgtgat tagttcgtcc attatttctc ctcctaaaat ttcatttcat cgttgttgtc
      781 atcttctttg tcatcagttt gggaaaggag cgcatacacg agatatgctg ctcctactaa
      841 acctaagaac aagagaattt tagccataaa gaatcccata ttatttgcct gcttcatccg
      901 taactacagt atctgctcca tttactgtta cccatccatg ctttttgcga gcttgagctt
      961 cttcataacg aatcaaattg tctgttactg attcggcgac tttgcgatta gattctgcct
     1021 ctgcttctgc agcttttgtt ttcttgtaag cttcactatc agcttgagtt tttgcagttt
     1081 ctgcgtctag cttcgctttt tcattttctt gaccagctcg aatgatcgca tcgattgatt
     1141 tttgtgtttc tttatcgaca tctggaacac caagtgttac gtcttcgact tcaaaccctt
     1201 tagattcaac tgatttagca aagttcgtta atacctcagc ttcaacttta gaggaatctc
     1261 ctgaaaggac atcaagcaga ctatatttcg cataaacttc acgagcgacc ttttgaagct
     1321 tagattttaa ccatccactt tcgatatctt ccgaagtgat attcccaaat tccttgtaca
     1381 tttttgctgc tttagttgaa tcaactttgt agtcatattt gatatcaatc gttgtctttt
     1441 tgccgtcgct tgtagatact gaaatatttt ttgattggat agtttgcaag cgaattggat
     1501 attggatcac tttgtcaatc cctacaaatt tcacaccttg cgtcagtgct tcatctttga
     1561 taccgccgtt cattgaatag cgcacaccca catatccatt atcgattttt tcgaagaact
     1621 taaatcctcc gataactcca atacctacga ttactgctcc tgctacaccc aatttgatta
     1681 atttgttttc gttcattttt cttcctccaa atacttatat tcgtgccctt ttcgatcggt
     1741 gtgatttcgc caagcgtacg ttcgaatagt cccacgcgta tatccagttt ctgcagacag
     1801 ctcactagct gttccttgca tcaaaatttt gccttcgtgt aaaacaacga cgattttccc
     1861 tttgcgctgt tttcttttgt ctggcttttt tcgtatacgt ccgttagcaa tctttcctag
     1921 acgctgaact tcagcaacca ctgcttcatc ttcttgccag ttctcatctt gaatcagcaa
     1981 cattaatttt cgccaagccg cttccttatc cacgctcatt cctccaatct acggatttcc
     2041 cttcttaaat tctctatgtg caaatcgatt gcctttctag ccgtttcatt gaccatcact
     2101 gcctttgttc gttccagatc gtcaatctca cgccgaaggc ttcgaattcg catttgaatc
     2161 acttcttctg ttgtcatgat ggaccacctc gttaaaaacg ctcttccttg aacgtattcc
     2221 gatatttttt ggctaatatc aacggcactt gatattgatg acagaacaac tttgccttga
     2281 tcttaaagtc ttttgtctgc atccccttaa catctacgac tttgaccagc ttgccgcttt
     2341 tgtaaaatgt gaagtcggga atatactcga tcttgcgata cttcttgccc tctagttcaa
     2401 acttcggcat cagctcaaac ctttcctgga gcttcacttt ccaaccattc gcttcagctt
     2461 gccacaaggc tagatcgtaa tactctgctt ccgcgataga atcaaacttg atacctcgat
     2521 gaacagtttt tttattacgg tatttattca tgcgatacta cctttcactg gttttatgcg
     2581 cttgtctgct gtttgagaaa ataccatcgt aaatccatca gagttgacaa acattcttga
     2641 tattgttctc gtaccgtaag cttttttcag ttcttcacca agcaagttcg ttgtgataat
     2701 cgttgctagg ttctgtcgtg catccaagaa cgaatttagc gtgtttatgc caaacgctcg
     2761 actatctgat gcatcttttc ctagttctga accgatatca tcaatgatga ctaaatccgc
     2821 tgttttgata tctgcaatca acgatccctc aatcgttttt ctgagttcag gattgttgta
     2881 cgaaaatttg atttgatcca gcatttcctg taacccgata aataagattt ttttatcgta
     2941 gtttgatcgc ttcaagactt cccaagccgc tgccatcgct aggtgacttt tacctgttcc
     3001 ttgttttcca gttaaaacaa ggtgacttgg atttcctaac aagactgaat taacaaagcg
     3061 tttggttact tcaaccgctt gtcttgtttc ttgatcgaca atttgataat tctgcaatgt
     3121 gcaatcaaac aacgcttgat taggcacaac agaaccacct ttgaaaaaat tgattgctct
     3181 tgctttcagg ctctcgttgt aaattcgttc tgtttgtaag tcttcctgaa ctcgcaatgc
     3241 tttataccca cattgcatac aggttggctt acaacgttct gagccatcag ggttcttcgt
     3301 acgccatcca tacaaaggct gtccgcattc aggacattcg ccacgttgga caagcacttt
     3361 ctgtatcagc ttttccatga tttccccaac agtttccatg cctacgcctc ctctcaaata
     3421 ggcaactcat ctgtgctaaa tttctcgtat tcaagcgttt tagtttgttt tgttttataa
     3481 tcacgatttg cttgcgctgc cattgtgtcg tatttttctc gcaatttttt agcagataaa
     3541 atattcgatg cccaaaacac attgtgctga ctccattcaa tcattccccg tacctggttt
     3601 tcggtccgct tgtcgatctc gatcattttc cgaatgttat ctgcccaact ttgcagattc
     3661 ggctttttga tttcctgatt ctgacaaatc tgtttgaata actcctccgc gagaatgtag
     3721 taaactgagt cggtgtcgta aacacgcttt ttgcgtggtt gcgacgatga tgttttatta
     3781 ttcttttcat tcttataatt cttttcattc ttgtttgtgt gcacttgttg ttcacttgtt
     3841 gtgcgtttga tgttcatttg ttgttcactg ctttggtaca aagaccagtt ttttattgat
     3901 ataacgctgt atttcgtagt tgatttgatg ttcaacattc cgtttttttc aaattgtttt
     3961 agccatctcc atacagaacc gctgttcact tgatgttcac gtttcacgcc ttcgttcatc
     4021 tcagatgtga tagcatcgcg ccctgtgacg aattctccgc tgttcacttg tatctctttt
     4081 ccattaaaaa gtatcttgcg attctcatga ctcgctttca tcaaacacaa gctccataat
     4141 ttatacatat aaggattggt ccaaacgaat gaattcatca cttttcgatg taatttgaca
     4201 tatcctgtgt tcattcgtat ttctcctcta gaaaagcaag ggagaaagct ccctcattat
     4261 ttgtttaacg gcggattaga tgcatcaaat aatccagttt gtacatcttc attttcttca
     4321 gatataacct ctgcttcttt tctttcagga atatcttctt caacttctgt ttcagcaata
     4381 atgctgccgt cttcttgaac cctttggact ctctcatccg atgtggtggc ttcttgcatt
     4441 tcgatggaca agattcccca tttagaaaga agatttctca aaacagtttt tcgtgccatt
     4501 gcattgtaat cagatgccca cacaccactt aactttgtct tatcgcgatc tttattgtta
     4561 gcaatccgat gagcttcgat ttcttgtttg gtccaataga cagttttttt gaatccattc
     4621 agtaactcga aatagccaac atatccaatg acttcatcag acgttctacc atttggatca
     4681 aactcaaact cttctgtcag tcggttccag ctttttagtt ctccttcgta aacttcgatc
     4741 acatttaatg ctttgtattt acctgatcgt tgggctaatt ggatatatcc tttatagcca
     4801 agcatgaatt gagctttctt ttcccatttt cctgtttgct tgtttttact attgaatgga
     4861 actaaatatg cataacctaa attcttatct agcccaagat ttaatgttgc agcagttaac
     4921 gcaccactca tgatagacat cggttcacta tctgcaagat aactgtcatt agatacaaga
     4981 gtcataacat tcgacataaa agcattagca ttgtcatgaa gtacttcttc aaatttcttt
     5041 ctcattgttg gtgtattcat tagagcttta agccctaact gtcctggtgc aacttgtttc
     5101 tgtggctttt ctgccaattg attttttaac gattcatttg ttgccatatt atttgatctc
     5161 cttttcggtt agccttcttg attcagtaac gttataaatc tcttcatcat ttgcgacatc
     5221 tggatatttc tctgctagtt tcttcgtgtt catccgtctt gtacgaacaa gtttccagct
     5281 gataatgttt ctttgtgtga taccgatact ggcttcacgt ttacctagct cactgatgat
     5341 ctcgttgtct acttgacgaa tagatgattc aatttctttt ttcgtccgct tgagttctct
     5401 tttttgctcg ataagttcat caaaacgtga tggtagagct gtttgatttt cttctacatc
     5461 tgcatatttt tcttttaaga agtcagcagt cgctccactt ccgtcaatta caggctcgat
     5521 acctccaaga acgttcgttt cccaaaactc tactaattgt tcagtgattg tatcgatcag
     5581 ctcttgatct cgttcaatcc gcttccaaat gaatttttgt ccaccgatca aaacagcgat
     5641 ataacaatag tctttgttta aaacgttcat gtaatgctga acttgacaga gatagctaag
     5701 cggtacctct tcaccttccc actcttttcc gagaaattga ttggctgttt tacattccag
     5761 aatggcattt tctcccacta cttcccgatc aatatttgct cttaaaaatg gatgtaatgg
     5821 atgttcaaat acttggtttc ttctgcgtac ttttttgcct gtacgttcct gaaactcttt
     5881 agcaaccact tcttctaaaa cattacccca ataagctggt tcgctttcta aatctttcaa
     5941 ttcaatttga cccgttttct caagccacaa ttgataagga gatttgtatt tgtttaatcc
     6001 aagtacggtt gcaacatctg agccaccaat acctcttttg cggtcttcga gccattcttg
     6061 atggctcatt tctaaggtag atttactcat cttcatcctc ctcatcaatt ggcgcttcat
     6121 atggtggttt agcataatca ggatcagtta aatagttgtc aagatttgct aactcgttca
     6181 tgtttaacct cttttctgct tgctaacacg caaagcacaa gcatggtaat tgcgaataac
     6241 aagccaaaga ttacataact tttcgctaca atcactaacg tgaaaaataa aataatgagt
     6301 agatcaattg ttttttcatt catcattgaa attccccctt gagaatacga gtcatcacat
     6361 cagctaaatc ttccttgtta ttaataacaa aagtatgatt tgaaggttga ttttcttctt
     6421 catttttcgc ttgttcgtaa gcatccacag caattctacg cataatatgt gctgacaatg
     6481 ggagattttc atctaaatgt tcttcttgca tagcaagaca aagtaaaatt tctgttgtct
     6541 caccataaac gagactttta agttcccctt ctttttgggc tgtacaaata atagaaacac
     6601 cttgcttttg gcattcacgt ttcaattctt cgataagatt ttcaattttc ttattcatgt
     6661 gataaaatct ccttaggtat aatttttggt ttgtgactca ttgctttgga cggctgagtc
     6721 acttttttat ttctaaccac ttcttctggc gttcacgcca ttgttcccct agcaatctag
     6781 gtttcatttc ctcgtggtat tcctgattta gttttcgcat caagtgttga tgctcttgta
     6841 atgtcatttg atccctcctt tcgtttattg aatttctcca tttctatttg ataaaatatt
     6901 tctatagaaa tggaggtgaa aactatggga aaaaatcaac atgttgtacc taattccaat
     6961 ggtggttgga atgtcaaagg tgctggaaat tctaaagcga ctgcacatac taatacaaaa
     7021 tcagaagcag taaaaatcgc tagagagatt tctaaaaatc aaggatctga gctatttatt
     7081 catggcagag atggaaaaat tcaaagccgt gatagtcacg gaaacgatcc acacccacca
     7141 aaaggttaat catatttagg agttaatcga atacgataac cgtcagcagg agttgcatcg
     7201 gtcaatgtaa cttctgctac tttttcccct tcttctgttt caataattaa tcttgtgtaa
     7261 ttgttctcat tcaaaatccc tatttttgat tcttccttca tgtcatccct ccttcgtctc
     7321 tcgccgtcac cacctccaag aagaggagtt gccaaaatga agaaacgtgg actttggcaa
     7381 acttgaaagg agagcagcaa ctcctctttt cggaagtggc gagtgtgtga tataatatta
     7441 ttgttctatg gtgcccacta cttgcttgcc ggctgtgtgg gcttttcttt ttgttcaatt
     7501 atggttgcca ccacagtaac tccccaatgt ctagaacgta ttctagccat ttcatctgct
     7561 aatctttgaa gattaggtgt gccattcaaa gtgatttttg gcttgttact cacaatctca
     7621 cctccatcag tttgttttct tttcacctct taatagaatt aacttgaaag cgaggtgaat
     7681 tgttatgggt aaaaatcaat gggtatctcc tagaaatggc aaatgggctg tacgtggtga
     7741 aggtaattcc gaagatacaa aactatttga taataaatca gatgctgttg agtatggtaa
     7801 aaagatttct aaaaaacagc aatcagagtt tattgtccaa aaaaagaatg gacaaataca
     7861 atcaaaagat agttttggaa acgatcctat acctccacgt gataaagaac attaatcata
     7921 tctaggggtc attcgaactc gaaaaccttc gcatgattca atatcattcg ccgtaataac
     7981 tgcgattgtt ttcgggttct gctcgtctgt ttcgacaaca atttttgtat attcttcaag
     8041 agtcggtact tttatattct tctcttccac tattcacacc tcctagctga cttgttttgt
     8101 ttcacctgtt attgatttcg gtttttccga atcagggtct aaaaaaatat cggaaactct
     8161 aacacccaat gccttagcaa ttttttctag agtggcgtac tttccgttcc tcattttatc
     8221 tacatcgttt tcgtaaataa atattgttct tgatgtcact ttgcttttcc ttgacagttc
     8281 ttcttttgaa taacccttta aagctcttaa ttgtctcaat gtatattttt ctttcttcgc
     8341 ggtatccata ttttattcgc ctcctctctt taactttctg actaaattat atacttcggt
     8401 ttttccgaag tcaagtgcat atttcagttt tcctgaaatc gatttctatt ttgcgtaaat
     8461 acttctttaa aaacactaaa atattttcta agaaacctgt tacatttttt ccgaaacggt
     8521 gttacaatta actcaaggag gtgttactat gttcgctgaa aatctaaagt ttcttagaga
     8581 aaagtataat attgatcaac aagaactagc cgaaaaacta ggaagaaaaa gttcatcctc
     8641 aataagcgaa tgggaaaaag gaaaatatac accaaaatta aaaaccttaa atgagatagc
     8701 taagatattc aatgttgata tagatgattt gatgaatatt gatttatcta tatctaatca
     8761 gatatataat atccttccta tatataatca gcttaacgac gaaaataaag ttgctaccta
     8821 tgaatttgca agtaatcgat tagaagaaca aaaacaagaa aatgtcctag attttcctaa
     8881 aaaagaaaaa atcccaactg ttcacaattc ggcttcggct gccaatccaa cagaacttac
     8941 ttacggtgat acggttgtcg aagaggaaga atttgagaga gttccaagtg ctgctgactt
     9001 cgcagtacct attataggcg attcaatgga acctgttatc aggaatggac aattcgtatt
     9061 cgttaaagaa caaccggacg tagaagatgg ggaaattgct attgtggaat taggtggtga
     9121 cggagttaca tgtaaagaga tatataaaga ttacgaaaat caaacaatca ttttacgttc
     9181 tataaacgac ttatacgaag atagaattgt atcgccagaa cagattagaa ttatcggaaa
     9241 agttgtattt taaaaaaatt cccatgccgg agccgtcaac ttcggtaagg gtttatatga
     9301 ttttgacaat tattttataa aagaagcgag gcgttttgaa tgggagtact attgttttta
     9361 gttggaatag ttttaataat tatgggtagt agcaaattat ctgaagctaa aaaattaaaa
     9421 gataaaactt ctgccttaga gagtaaggta aaggaatatt ctcaagaatt aaatgctagg
     9481 gaaacgttta tatctgaaaa agagaaaaac ataagtaaat tgaataaaaa cttgtcaacg
     9541 ctagaaaaca aaattgatat tcttaatgct gaaagccagc ggttagacga tgaaattaat
     9601 caaaaatatg acacatctct ggtcactatc tataaatcag taattattcc gaatatagat
     9661 gaaacttctt catccgaaat aaaaaacaaa ctttctttat taaagattaa agaaaaggat
     9721 tttattttag acggtgcaat taatattcct tcttatttga ataatagaga agctaataat
     9781 attaaaaaga aaatgcttat accattagaa tcagaaataa ctggacttct caataaactt
     9841 actatttcta acgtagattc aactagagaa aaaattataa gaacttttga taaaataaac
     9901 aagcttttta aaaacgatga agcagaattc aaaaaagaat tacttgaatt aaaacttcaa
     9961 gaattagaac tcaactattc atacatagtt aaagttaacg atgaaaaaga acagcaaaaa
    10021 gcaataaaag aacaaatgat tgaagaagaa aaggtaagac gtgaaataga acgtgaaaag
    10081 aaacgcattg ataaagaaga acgacagttt aactcagaga taactaagtt aatggcatac
    10141 atgcaaaaag ctagtgctga tgttgaaaaa gaactctatg ctgataaaat taaagaactg
    10201 gaagagaaat taagagaact agaaactgtc aaagaagatg tattgcaaag agaacttaat
    10261 actagagctg gctatgttta tgtcatcagt aatatcggat ctttcggtga agatgtctat
    10321 aaaataggaa tgactagacg gttagaacct atggatagaa ttaaggaact tagtagcgct
    10381 tctgtacctt ttgaatttga tgttcatgca atgatatttt cagaagatgc accaacatta
    10441 gaaaatcaac tacattcgca ttttagaaaa aacgaagtca ataaagttaa tcaacgaaaa
    10501 gaattcttta aagtatctct tgatgaaatt gaaaaagttg ttttagaaaa ttataacgga
    10561 actgtatctt ttactaaact agctcaagct gaacaataca gaagatcatt agaattaaca
    10621 gatgtgtagt aaaaagatta gccttcgggc ttttctttta caacgataag aacatacatt
    10681 cgaaaggaga taacatgaaa cgagtagcat tatatatgag agtatcaact gaacaacaag
    10741 ccaaacatgg agatagtctt agagaacaaa aagaaaccct ctatgaatat atcgaacaac
    10801 acaaggattt gaaagtagtt aatgaatatg tagacggtgg tatttctggt caaaaaataa
    10861 atcgtgatga atttcaaaaa ctattacaag atgtaaaaga aaataaaata gatctaattt
    10921 tatttacaaa attagataga tggtttagaa atctacgaca ttatttaaac actcaagaaa
    10981 ttttagaaaa acacaatgtt tcttggaatg ctgtttctca acaatattat gatacaacta
    11041 cagcgtatgg cagaactttc attgcacaag taatgagttt tgctgaattg gaagcccaaa
    11101 ttgattcaga acgcatcaaa gctgttatgg caaataaaat tgcacaaggt gaagtagtaa
    11161 gcgggaaaac tccgttaggc tactctatag aaaataagaa attagtaata aatgatgatg
    11221 ctcctatcgt gatagatata ttcaactact ttctttctag tggtagttta agaaaaactg
    11281 tttattattt aggctctcag tatggaatcg ttagagatta tcaaagtgtt aaaaatatgt
    11341 tgacgaacaa aaaatacatt ggtgaattaa gaaataataa aaattactgt cctcctatta
    11401 ttgataaaaa acttttttat gctgttcaaa aagccttacc taaaaattta aaaacaaatg
    11461 caaaacgtga ttacattttt aaaggtttat taaaatgttc tgactgtcag ggatcagtag
    11521 ctggtcaaac tattaaagca agatataaga aaaaagacgg tacagaatct atatacgaaa
    11581 ggacttgtta tagatgtgta aaacgaagaa ataataaatt acgttgcaca aataagcgcg
    11641 ctttttacga aaaaaattta gaacgttata tttttgaagc tactaaacaa aaatttgaac
    11701 aaatacaaat aaactattca aaaaaacaac caaagattat taaaaagaaa aatagtaaga
    11761 aaaatattga gaataaatta gatagattga agaaagccta cttgaatgaa gttatagact
    11821 tagaggaata caaaaaagat cgagaagctt taatgaaaga attaaatgaa attgaagtag
    11881 agccagctaa aatagatata aaaaatgtcg aatttattct atcaaaagaa ttcgatgaaa
    11941 tttataaaga atcttcggaa gaagaaaaaa atgcattatg gcgttcaatt atcgataaca
    12001 taatagtatt tccagatggc aatataacag taaatttcct tatataattt atgtgtacta
//
LOCUS       DACCDF010000082        11860 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCDF010000082 DACCDF010000000
VERSION     DACCDF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.085"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK51_002273"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVK51_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP6431837.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK51_002274"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVK51_002274"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP6431838.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVK51_002275"
     CDS             complement(2169..2906)
                     /locus_tag="IVK51_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6431839.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVK51_002276"
     CDS             complement(2997..3524)
                     /locus_tag="IVK51_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP6431840.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVK51_002277"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVK51_002277"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP6431841.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVK51_002278"
     CDS             complement(4401..4886)
                     /locus_tag="IVK51_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP6431842.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IVK51_002279"
     CDS             complement(5039..5236)
                     /locus_tag="IVK51_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431843.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK51_002280"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IVK51_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP6431844.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK51_002281"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IVK51_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP6431845.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IVK51_002282"
     CDS             9211..9423
                     /locus_tag="IVK51_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP6431846.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IVK51_002283"
     CDS             9425..11104
                     /locus_tag="IVK51_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP6431847.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IVK51_002284"
     CDS             complement(11358..11558)
                     /locus_tag="IVK51_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP6431848.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..>11860)
                     /locus_tag="IVK51_002285"
     CDS             complement(11708..>11860)
                     /locus_tag="IVK51_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HAP6431849.1"
                     /translation="GNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRYFSLREE
                     GMMEEK"
BASE COUNT         3428 a         2567 c         2064 g         3801 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc
//
LOCUS       DACCDF010000083        11434 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCDF010000083 DACCDF010000000
VERSION     DACCDF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.086"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(362..1480)
                     /locus_tag="IVK51_002286"
     CDS             complement(362..1480)
                     /locus_tag="IVK51_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431850.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1951..3258
                     /locus_tag="IVK51_002287"
     CDS             1951..3258
                     /locus_tag="IVK51_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP6431851.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3377..4576
                     /locus_tag="IVK51_002288"
     CDS             3377..4576
                     /locus_tag="IVK51_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP6431852.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4603..5871)
                     /locus_tag="IVK51_002289"
     CDS             complement(4603..5871)
                     /locus_tag="IVK51_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP6431853.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6034..6680)
                     /locus_tag="IVK51_002290"
                     /pseudo
     CDS             complement(6034..6680)
                     /locus_tag="IVK51_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7088..7591)
                     /locus_tag="IVK51_002291"
     CDS             complement(7088..7591)
                     /locus_tag="IVK51_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP6431854.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSPSMRKRWQRY
                     FGKESSI"
     gene            complement(7714..8478)
                     /locus_tag="IVK51_002292"
     CDS             complement(7714..8478)
                     /locus_tag="IVK51_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP6431855.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8586..8861
                     /locus_tag="IVK51_002293"
     CDS             8586..8861
                     /locus_tag="IVK51_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP6431856.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9299..10249
                     /gene="yidC"
                     /locus_tag="IVK51_002294"
     CDS             9299..10249
                     /gene="yidC"
                     /locus_tag="IVK51_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP6431857.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10428..10628)
                     /locus_tag="IVK51_002295"
     CDS             complement(10428..10628)
                     /locus_tag="IVK51_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431858.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
BASE COUNT         3446 a         2398 c         2020 g         3570 t
ORIGIN      
        1 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
       61 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
      121 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
      181 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
      241 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacgga aaaatatgtg
      301 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
      361 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
      421 taaacacttt cccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
      481 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
      541 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
      601 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
      661 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
      721 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
      781 gtaatgcaaa taaataaatc ggccattgtt caggattttc gaaagaatcc ttcaagtacg
      841 tatcaacagc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
      901 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
      961 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
     1021 gagatacttc ttttacttgt ttgttgctga cctcatacaa tttcatggag tcgcggttca
     1081 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
     1141 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggtct
     1201 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
     1261 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
     1321 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
     1381 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
     1441 aagtcaattg tgtcaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
     1501 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
     1561 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
     1621 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
     1681 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
     1741 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
     1801 ctattcctgt aaaagtacgt aaaaacgacc gctaaacttc cataacgcgc gttcttatcc
     1861 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
     1921 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
     1981 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
     2041 gaagatacgt tcaaagtaaa tgcaaaagca gcttttgctg tagatgcgca aagcggtaag
     2101 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaatcatc
     2161 ggtctttaca tcgtccttga tcaagtaaaa gaaggaaaac tgtcatgggg tgataaagtt
     2221 tcgatttcag attatgcaga aaatttgagt atcaccccag atttatcgaa tgttcctttg
     2281 cataaagaaa acacctacac tgtcaaagaa ttattcgact ctgcaatcat ccagtcagcc
     2341 aatgcttcaa tggtggcctt ggcagaaaaa atctctggca gcgaagctaa attcactgaa
     2401 cgaatgaaag aacaattgaa agattgggga atcaaagatg caacaatcgt taatgcatca
     2461 ggattgaaca actcttatct tggcgaaaac cgaccagaag gcactggaga aaacgacgaa
     2521 aatcaaatgt ctgctcaaga tgtggccatc gttgcccgcc atttgatctt ggatttccca
     2581 gaaatactgg atgtatcaag tactacgacc caaatgtttg gcgaaaatac gcaatcacca
     2641 gtggaaatgg tcaactggaa ctggatgctc ccaggattcg ttaattacaa ggaaggcgtg
     2701 gacggattaa aaactggtac gactgagctt gcaggtgctt gtttcgttgg tacgatcact
     2761 aaaaacgatc aacggatcat cactgtcgta ttgaatgcgg acggccatgc ggaaaacccg
     2821 agtgcccgct tcaatgaaac agaccgtttg atggactact gctatgacaa ttggtcagaa
     2881 aaagagctcg gaaaagctaa cgcaagtatt cctagtctaa aaaccattga tgtaaaagac
     2941 ggaaaagaat caacagttcc tgttgtgctt aaaagccctg taaaagtatg ggtacgaaac
     3001 gatatggata ctggcaaatt aacgatcacg ccaacacttg acaagaagca agtcactgac
     3061 aatgagatcc aagcacctat ggataaaggt acaaaaatcg gtaccgctac gatcaccttg
     3121 gcagacgata aattaggcta tttagaagac aacaatgcac catcaacaga gatcattacg
     3181 gataaaacag tggaaaaagc gaatatattc gtgattggct ggcgacgcgt cgcagaattt
     3241 ttcggcaact tgttttaatt gaacaataag aaaaacatct ttccatcgga aagatgtttt
     3301 tttgcttttt ctagtttaaa taacagcttt tttcatctat tttcgttata ctgtataaga
     3361 aaaaggagtt gaatttatga atctaaccag agatctgatc tttttagttc tctatcttat
     3421 tggggtattc tctatcgttt attggcgaaa acagaagaag gatccttcct tgttctttta
     3481 cggcagtacg tggacattta cggtaatctt aacttatttt gtcgtgttgg aaatttttta
     3541 ttcgaccttt tattttttga ttccattgtt gttctttagt atctttgctt tttcttattt
     3601 cacagaaaaa cgaaggctac tgaatggtct tttattcaat gttttactca tcagtttcgg
     3661 catctacttg tttgtcttgc tgtatgaaac gcaaaatatt tttcttggcg gactgatcgc
     3721 cttgatcacg attccattgc tacttgtcct tttatttggt atttatggat tgattgtttt
     3781 tcttttttgg aacggggtca cagttttacg tagagaatcg cattcattag ctaatctttt
     3841 aacgttgata ctagccattt ttcttacact gtttcttgtt tttgactttt tcttgttaaa
     3901 atatttgccg caatggataa atgctttgtt tttctgcgtt cccctgatct tgatctatct
     3961 atttatcgta ttctacaact ttttgactgt ctcattcttg tatcagttca accggcctcg
     4021 atacaatcaa gacttcatcg ttgtgttagg cgctggattg atcaatggag aaacagtttc
     4081 tcctcttcta gccaagcgaa tcaataaggc aattgctttt tatcgggctc aaagccgtgc
     4141 tacattaaat ccgcccatct tattgatgtc tggcgggcaa ggagcagacg aaaaagtacc
     4201 agaggccatt gcgatgaaac aatatgccat ggaacaagga atccctgaaa gagacattct
     4261 tgtcgaaacc aattcgacaa ccactttgga aaacatgctc tattcaaagg aaatcatgga
     4321 tcagcaaatg aaagggccat accgcgcgat ctttagttcc aacaattacc acatttttag
     4381 agccggtctt tatgcgcggc aagccaaatt gaaagcaaat ggtattggtg caaaaacagc
     4441 tttctactac ttgcctaatg cgtttttaag agaatacctc gccatcttga tgatgaacaa
     4501 aaaaagacat atgatcattt gcggattgct tttcgcaggc tccgcattct tatcgctgat
     4561 tactttactt ttctaaaaaa acaaggtgtg acaaaacata tgtcacacct tgtttttttc
     4621 tttcattctc ttaactattg gcaaaaagag tatccctttt ttgaccgcta cttgctgagc
     4681 agaataaata ccgctgttgc ctgaacacat aaagctgatc aagcagatca taaagaagta
     4741 tactgccgct tgtgaaccga acaattcgat ccccatcaca aagcaggcaa tcggtgtatt
     4801 tgtcgcacca gaaaatactc cgataaagcc caatcctgca agaaaaggaa cagaaacatg
     4861 gagcaaaggc gctagtgctg caccaagcgt tgcaccaatt tcaaacaatg gtgtcacttc
     4921 tcccccttga tatcccgcgc ctaaagacaa aactgtgaag aataacttcc caataaaatc
     4981 atagacatgt gcctgtccat taaatgattc ttgaagtaac ggaagactca atccgagata
     5041 acgttgtgtt tgcaaaatca atgctgctgc cacaactaca gctccaccta tcgtatttcg
     5101 acaaacagga tgaggaatcc attgtgcata tcgcgctttt aaaaagacga tcgaacggct
     5161 gaatacccaa ccaatcaaac caaaagcaat cccagcaaca aaaactttga agaacagcaa
     5221 catactccat tcaggaatca cacccatttg gtaatgtaga tgagaaacac catagctttc
     5281 tgtcatgaaa tttgcaaaca gtgcagcaaa gaagctaggg aagatcgctt ctgttcggac
     5341 tttcccgatt gccaacactt ctaatccaaa taatgttcca gctaaaggtg tgccaaatac
     5401 agaactaaaa cccgcactga ttccactgat aatcaagatt tcccgttctg atttatccaa
     5461 tcggaacaaa cgtgccgtat tatctgctaa cgctcccccc atttggactg ctgttccttc
     5521 acggccaaca gatcccccaa ataaatgggt agttatggtt ccaaacagtg taagtggaat
     5581 aagccgcaac gggatttttt cttctccacc atttccttga tcgatcacta agttgtttcc
     5641 acgactagaa agtccgccgt gatacgcata gagataagca aaaagcgcac cactaactgg
     5701 tagtagacaa aggagccaag gatgtgatag tcggatatct gtcacaactg tcaggctatt
     5761 taagaaaaaa gcagacaagc tccccatgaa tataccgaga atcgctgtga tcaccagcca
     5821 cttcagcata tacactaaaa tgcgtactgg actcggtaat tgtttttcca taaaaaattc
     5881 ctcctcaata aaaaaacgcc tactaaagaa aggggctgtc cccagacttt agtaggcgtc
     5941 attagctatc tttcgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     6001 agatagcata tttttctgta aaaaacaata aaattatcgt tctttcttta cacgttcagc
     6061 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattggtcac tatcatgaca
     6121 aatataattc ccctttttcg tatacgtgcc atcagaacct gctttcgttg tagaaagaaa
     6181 aagcgaaaca ttcgatgttt tctgacagat tgcacagatt cccttattta tcgttggtga
     6241 taaagtaccg ctaatccctt ttaatttttc atcttcgtaa tagaccatga attttttctg
     6301 cgtacctgga tcattccagc cgatgtacgt atgttcttgc aagtcgattt cattccaagc
     6361 tggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctaggctg
     6421 cataaacggc tgaacgattt gcttcagctc ctccaatgct ctttctgctt gcgcttttgt
     6481 cagttttacg tctaaaactg tcgttaaaaa tgtatcgatt ctggcagttc gtgatctaat
     6541 atgtctcgaa tcttttcagc tgctagtgcc tgaactgttt gtaccgtttt tggatcattg
     6601 acagatgagt acgcatttaa taactgttct acttctctta ttataaaaaa atattggtat
     6661 ggttttattg tttgttccat gattttaaag tcctttcttc taaccaaagt gcatactttg
     6721 tagaaaaaag tgatcttctg caaagtatgt gttacctgaa gatcccggac tcttttaaat
     6781 aagatacgtt ttgcatctta tcatcacccc ctttaatgct tattattcta tgtacatgat
     6841 tcattcgata aataaaagta aaccgtatct tgaacagata gtcaactgca aacagaaaaa
     6901 aggactgtga caaaagtcgt gtcaaagtcc cttgtttcga atgaatggtg ttcaaaagct
     6961 ggcatctgcg gtaataagcc gaaacttcac aaaaatttga gaagcaattt tcgtaaattc
     7021 cttcttattt cttgaagctg accacttctg tcacaacctc aatatggtct attattggtt
     7081 ttatctatta aatcgaggat tctttcccaa agtaacgttg ccatctctta cgcatacttg
     7141 gtgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     7201 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     7261 ctcgctcaag atccgacaat tcggtgatct tttctgcatt ctttacagca attcttggat
     7321 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     7381 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     7441 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     7501 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     7561 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     7621 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     7681 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     7741 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     7801 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     7861 tgcgaagtta tctgattttt ctactttgtc taatgcgtgt gcttcttcat ttaattctgt
     7921 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     7981 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     8041 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     8101 catcgtccct acatttccag gatcttgtac attatctaac aacagccatc ctcctgaaaa
     8161 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     8221 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     8281 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     8341 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     8401 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     8461 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     8521 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     8581 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     8641 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     8701 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     8761 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     8821 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
     8881 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
     8941 acttaatgcc gtagatgcaa gcttttgaac accgtcgata aggttattct tgaggcgctt
     9001 tgcctcaaaa attaaggaca gcagtgaaaa agctgatcta tcacttgcag tgagttcatt
     9061 tgactccgct ttttcgggca gaaaaaaagt ttttagttta ttaaagtaag aaagaaacac
     9121 ttggaatcat tctacttttt cctagtattt ctttcttgat tttttgtctt tttttcaaaa
     9181 tttttcgcta cgtaaaagaa cgaatattta tatgaaattt ctatgaagtt gattgaattc
     9241 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
     9301 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
     9361 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcgggaatcg tttatcgagt
     9421 actcgtccat cctatgggcc aagcgattac atatctagtt gaaaatttca attggtctta
     9481 tggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat
     9541 tagtcaatcg aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
     9601 tgaagcagcc caacaaaaat tgaaagctgc aacaacacgt gaagaacaaa tggcagcaca
     9661 agcagaaatg cagcaagtat accgcgaaaa cggattgagt atgaccggcg gaatcggctg
     9721 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tactttacgg cacgttatac
     9781 agaaggtatc cgggaatcat ctttctacgg catcgattta ggctcaccaa gtatggtact
     9841 tgttgccatt gcaggggtcg cttatctgct tcaaggatac atctcaacga tcggtattcc
     9901 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtctt
     9961 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtcggtg gtgtgttcag
    10021 ctgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
    10081 agaagagttg aaacaaaacc caccaaaaca agtcgtcact ccaagaattg atgtgacaga
    10141 atcagctaaa ccagctgaca gcaaacaagc aaccaaacaa atcaagcaac ccaaaaacag
    10201 tacaaacaat aaaggccgca acgcaggaaa gcagcagaag cggaagtaga tagtaaataa
    10261 gagtgtaata aaaattcatt aacagtttta gcaattaaaa atatcgacca agatttttga
    10321 aaaagaaatc tggtcgatat ttttatttaa gtgaatgttt aatttgaatc atcgattgat
    10381 cgctatctaa ttgcagcagc atctttttat gaagaaacaa ctttttctta tctatcaacg
    10441 tagacaggtg ttgctgtttc ttctaatagt gtacttaatt ccttaatatt ttgttttcct
    10501 tcttctaaca tccaactact cgctttttct tcgccttcgg taacaaaagg cgccactccc
    10561 ttcgcccatg ttgctcgccc acataagaca ccattctcca taaaggacaa cgcggagata
    10621 ccacccatct aaataacatc cataaacctt tctagtagaa gaatagtgat agtatccatc
    10681 cctttttcta ctgggaatac aattacacga ttcaaagctt gatacacaac atttcccttc
    10741 ttataaaccg ctattgtaac cgaattgttt tgaatcacct ttttgggata gaaactggct
    10801 cctttccgca acttcttaaa tgttggtttc ttttatactt tctttgtagc aactacaaat
    10861 ttcttcaaat ctctaaatgt ttgcataaca accgcctttc tgacttttac tcaggatggt
    10921 atttaaaaat cagcaaaata aggcttctct ccatcttttt tatttcctag ctgctttaaa
    10981 gacactcttg ctgtttttta atctctatca agggggctct gaatctattt tgatgaactt
    11041 atcttattaa tttctcaaat gagctgtcag acacaactgt attttttcaa tctcataccg
    11101 gacaactatg ccagcttaaa aaaatttcct ctggcaaaac ttctttattg aaaaatgaca
    11161 atgaaaaaag accattcttt ttgttgaatg ctcattgtgt aaacatattt cctgcgactg
    11221 ctcttcgaac cattctcagt gcaatcccta atttatctat taaatcttct tttagttcct
    11281 catctttcat attcaaaatt tcctttgtat gttacacaag tttgttctct aaataacgcg
    11341 aattgtaaaa ttaagttaga aaaataaaaa ggcatttatg gtacactcaa attgtatttc
    11401 cgacgaaaga aaacaaagga gtgtaaccat aaat
//
LOCUS       DACCDF010000084        11340 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCDF010000084 DACCDF010000000
VERSION     DACCDF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.088"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..936
                     /locus_tag="IVK51_002296"
     CDS             <1..936
                     /locus_tag="IVK51_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6431859.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="IVK51_002297"
     CDS             939..3122
                     /locus_tag="IVK51_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6431860.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="IVK51_002298"
     CDS             3119..4120
                     /locus_tag="IVK51_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6431861.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="IVK51_002299"
     CDS             4135..5049
                     /locus_tag="IVK51_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6431862.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="IVK51_002300"
     CDS             5294..5410
                     /locus_tag="IVK51_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6431863.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="IVK51_002301"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="IVK51_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6431864.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="IVK51_002302"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="IVK51_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6431865.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="IVK51_002303"
     CDS             9380..10642
                     /locus_tag="IVK51_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP6431866.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11340
                     /locus_tag="IVK51_002304"
     CDS             10887..>11340
                     /locus_tag="IVK51_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6431867.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKI"
BASE COUNT         3735 a         1897 c         2376 g         3332 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa atggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaggat ttcgccgaat gatgcaatat aaaaaaatta
//
LOCUS       DACCDF010000085        11253 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCDF010000085 DACCDF010000000
VERSION     DACCDF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.089"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(40..1419)
                     /locus_tag="IVK51_002305"
     CDS             complement(40..1419)
                     /locus_tag="IVK51_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HAP6431868.1"
                     /translation="MTKVTLKKVNPPKKDNEAKIYQLTDETMKFRKEKVLAAMEKSEI
                     DTLVIYCDLEHGGNFSYLTGFVTRFEESLLVLHQTGEAFLVLGNENTKMVDYSRIEAE
                     LIHTPLFSLPDQPMDNEKPLTEVLTNAKISKGKKVGLVGWKMFTSEEDNYQIQDLPAF
                     IVSSIVELVGEDAVSNRTDLFIHSDYGVRSLNTANEVAYYEFGSSLASDCVMNAIDEV
                     EVGKTEMEIGNHLVKYGQPNNVIPIAATGERFENAYIYPTDKQVKLGDKMSITTGFKG
                     GLASRSGYAVATTDDLPEGQKDYIEKVAAPYYDAVVAWLENIKIGMQGKELYSVIGKV
                     LPKSEYKWHLNPGHLTADEEWMSSPMKKDSEITLKSGMLLQIDIIPSVAGYAGASCES
                     GIALADESLRDQLAKEYPNVWSRIISRQEYIRNELGIELPDEVLPLSSGVGYYTPFFL
                     EKNLALVKG"
     gene            complement(1440..2987)
                     /locus_tag="IVK51_002306"
     CDS             complement(1440..2987)
                     /locus_tag="IVK51_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6431869.1"
                     /translation="MGPLSIIVASGLLLGIVSILQNPTLVGESFANATVIQGFVGGVQ
                     AIVGTMFGLLPILFAISVAIGMSREDKEIAAFSVVIAFILFHVVINYLLQLRGITAES
                     TSVETLMSDGMSQIKAVETSNMYETLLGTFTYRMGVFGGIIAGLWTAMIHNRYHTKQL
                     PAAFSFFSGNRFVPIMIILTIPIISIASFFIWPFVNTAINALGGLIGKSGVIGTFLYG
                     FSERLLIPTGLHHVLNQLIRFTPIGGTATINGETVSGALTIFNTELALPVKDMDILRS
                     ATRFLTQGTHPFMVFGLPAACYAMYKTSYLAQRPKIKGMLIAAAATCFVTGITEPIEF
                     AFIFISPILWVFHAFMAGLSFMLMNLFGVAVGNAGGGFIDLAIFGILQGTYTRWYLVV
                     ALGLVYAVAYYFVFKYVIERFDVKTPGRSNDDVLEDQDEEMTENELGKLILQGLGGAG
                     NIQEIDNCISRLRLVLKDTATIDEHILKKTGSMGIVKINENNLQVVYGGQVENATRSL
                     KNEMKRK"
     gene            complement(3236..4015)
                     /locus_tag="IVK51_002307"
     CDS             complement(3236..4015)
                     /locus_tag="IVK51_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6431870.1"
                     /translation="MTLEQLVNEHHSKLNENDDYVLSFITQNMEISISQTVSELAKQA
                     NVSDSTIIRLTKKLGFKGYGEFRYYLKEEFARMQKTRVDKSSLFLPEVLLGDVQATTK
                     LFEDDKSVEDIYETIRKADKIFAYATGYGQRMMLNEFARCMWNLGIYIIVVPEKNELD
                     LISKALTSNDLLFVISLSGRVDRIDDTLRRIHMKGTQLVSVTRFGQNNLASLSDYSLY
                     YQVTNINDVNELNNSSFCTLNLALSLLYEGYINYYKQSSVD"
     gene            complement(4090..4548)
                     /locus_tag="IVK51_002308"
     CDS             complement(4090..4548)
                     /locus_tag="IVK51_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6431871.1"
                     /translation="MGFFSRKEKLVAPVSGNLIPLSQVSDPVFSQGMMGEGFAVEPTN
                     AKVVAPIDGVIKSIFPTKHALTLTSNQGRDLLIHIGIDTVQLKGVGFQVLVEEGQRVS
                     SGESLVIFDCDQIKAEKLSDVVMVLFPEDKGIKLEVSEKKVSEGELLFKI"
     gene            complement(4560..5819)
                     /locus_tag="IVK51_002309"
     CDS             complement(4560..5819)
                     /locus_tag="IVK51_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HAP6431872.1"
                     /translation="MKTNFERIKKDIENLAEYNATPGEGVTRSAYSKEDREARNYLIN
                     QMEQLGLEIWEDGFSTLFGRRAGKKADAPSVMIGSHYDSVVNGGRFDGVAGVVTALET
                     MRILEENNFENDYPIDLILMNAEEGETFGPSTGVSNSRAMMGTLTMSELETVKNRHGQ
                     TKLEALRAYGVEPDLEAAKRDPKTIKNFIELHIEQGPVLEDEGKDIGLIEFIPGIGRF
                     KARFYGETEDSTAPMNKRKDALVAASKFVLFVEQWMKNLGEGIAGTVGKLTIKPNSNQ
                     FVPDFVEALIEIRTFDTKTTNPQEIKEKLLNELSVIQGETGVRTELEEMARINYSNPT
                     NPSVMDQGNVEKMKHICDQLGYSYQVINNGTGHDAMMMTDFVPTNMIYIPSKGGITHH
                     PDEWTDFEDVKKGADVLLNLVMDISKD"
     gene            complement(5935..6249)
                     /locus_tag="IVK51_002310"
     CDS             complement(5935..6249)
                     /locus_tag="IVK51_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6431873.1"
                     /translation="MDYEIQKFISEKEDQYFDRKSARIKPRDIIKHIVAFANADGGKL
                     VIGIEDDGTISGFKFQGAIDPWICVNKVDAFIESFLNQSTLELCYETRKSSYGGIYDN
                     DF"
     gene            complement(6395..7891)
                     /locus_tag="IVK51_002311"
     CDS             complement(6395..7891)
                     /locus_tag="IVK51_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6431874.1"
                     /translation="MNHQEAKQFFLKTSSFFSTSLPNYIDLSVGIEKAVDLLKNKPFT
                     ELVRHKRSLSEAVDVNHRILANKDGNYAWRPLQILHPLVYVDLVNGITQKNNWNHIKN
                     KFEEFQADKRIECISLPVESISSKSDTAETILNWWENLEQAQISYALEYDYCIHSDIT
                     DCYSSMYTHTIPWAVHSKEWAKTHRKDTQGVGNLIDSKIRYLQNGQTNGIPQGNVLMD
                     FVAEIVLGYADKMLLNKIEKTEIKEFKILRYRDDYRIFSVRKDQAEVIIRLLSEVLSD
                     LNLKLNSNKTFLSDNIILDAIKPDKIYWDLKHASFTEKSNNKWKFKLSLQKHLLQVKL
                     LGDKYPNCGSLSKALSEVYTHRVSTLNKRPDDIYQLISILVNIMQKNPRTLEACIVIL
                     GKLFEFIAVEEINLYLDKILRKFVNTPNTDIVEIWLQRLSLIHSREKSYSAKLCKKVS
                     NPKGFTLWNSDWLKKGFDESGIVNETFISNLSLTTPAKDLELFISQYE"
     gene            complement(8069..8548)
                     /locus_tag="IVK51_002312"
     CDS             complement(8069..8548)
                     /locus_tag="IVK51_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HAP6431875.1"
                     /translation="MKVYTVQPVDFLQSVDSEGFIYPKKRYTELSVFFDNPNAVGALH
                     WLEEEYTKRIQLPLDRDLIWLWRDTCHLKYARKDNFTDFSKYNFYCFEIPIEFYRKNI
                     LWSRFIEWHEPLNHEWETDENQEFEHIFDIPKHPGYGDIQGVTTRLHKDWIKKITHN"
     gene            complement(8924..9169)
                     /locus_tag="IVK51_002313"
     CDS             complement(8924..9169)
                     /locus_tag="IVK51_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6431876.1"
                     /translation="MLFLKEEKLIDKENVIGSNIRRIRLEKGMGQTELVRELQLSKIA
                     MTRETLVKIEGGRQHIKLDQLRAIRDILQVSYEDLLQ"
     gene            9274..10047
                     /locus_tag="IVK51_002314"
     CDS             9274..10047
                     /locus_tag="IVK51_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431877.1"
                     /translation="MKKFAIILTCALLTGAISGCATSDTNSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSESNSIEKVLPIEIEKNQENIKLKAELPILNSTEIDW
                     HENIKVTIEKGALAQLYTEKVEQFFESGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMTLKKIKAYDHPLNLTLNNGLLSFVLAGYVDYEYSPDYLETPLQHENNDINFR
                     FVYESKSKTWLVDQLSTINTSFHELPKPENEADMVIKIF"
     gene            complement(10276..10524)
                     /locus_tag="IVK51_002315"
     CDS             complement(10276..10524)
                     /locus_tag="IVK51_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6431878.1"
                     /translation="MDTITPTNFKKNIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDLMKNEKEDDFKVV"
     gene            complement(10623..10937)
                     /locus_tag="IVK51_002316"
     CDS             complement(10623..10937)
                     /locus_tag="IVK51_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431879.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFRLFRDQIIGISQT
                     TK"
BASE COUNT         3500 a         2317 c         1684 g         3752 t
ORIGIN      
        1 tcatttcttc cgttttaaag aagaaatgat tttcatagat caacctttaa ctaaagctag
       61 attcttttct aaaaagaatg gtgtgtagta gcccacaccg ctggacaacg gtaaaacttc
      121 atccggcaac tcgatgccca gttcgttacg gatgtactct tggcgagaaa tgattcgcga
      181 ccaaacattt ggatattctt tggctagttg atcacgaagc gactcatcag ctagcgcaat
      241 cccactctca cagcttgcgc cggcatagcc agcaactgat ggaataatat caatttgtag
      301 taacattccg gacttaagag taatttctga gtcttttttc attggcgaag acatccactc
      361 ttcatcagcc gtaagatgcc ccggatttaa gtgccatttg tattccgatt taggcaagac
      421 tttgcctatt actgaatata gctctttacc ttgcattcct attttgatat tttccaacca
      481 tgcgaccacg gcgtcgtagt atggagctgc aactttttca atgtaatcct tttgcccttc
      541 tggtaggtca tctgtcgttg caactgcata gccacttcga cttgctaatc cgcctttaaa
      601 gcccgtagta attgacattt tatcgcctag tttaacttgt ttatcagttg gatatatata
      661 ggcgttctca aaccgttccc cagttgcagc aattggaatg acattatttg gttggccgta
      721 cttaactaag tggttaccaa tttccatttc tgtttttcct acctctactt catcaatagc
      781 gttcatcaca caatctgaag ccaaggatga gccaaattca taatatgcca cttcattagc
      841 agtatttaat gagcgtactc cgtaatcact gtgaatgaac aggtcggtgc gattgctaac
      901 ggcatcttcc cctactaatt ctacaatcga acttacaatg aatgccggca aatcttggat
      961 ttggtagtta tcctcttctg aagtaaacat cttccaacca acaagcccaa ctttcttacc
     1021 tttactgatt ttagcattgg tcaatacttc agttagtggt ttctcgttat ccatcggttg
     1081 atccggtaat gagaagagtg gcgtgtggat taattctgcc tcaattcttg aatagtccac
     1141 cattttagta ttttcatttc ctagaactaa gaaagcttca cccgtctgat gtaataccaa
     1201 caagctttct tcaaagcgag taacaaaacc tgttaaataa gagaagttcc cgccatgttc
     1261 caaatcacaa tagattacga gggtatctat ctctgacttt tccatcgctg caagtacctt
     1321 ttctttacgg aacttcattg tttcatcagt caattgatat attttagctt cattatcttt
     1381 tttaggtggg ttaacttttt ttagagttac cttagtcata ttgtcactac tttctgtttt
     1441 tatttacgct tcatttcatt tttcagagat cgagttgcgt tttccacttg tcctccatat
     1501 accacttgta aattattttc gttaatttta acgattccca ttgagccggt ctttttcaag
     1561 atatgttcat caattgttgc tgtatcttta agaactagtc tcagccgaga gatacagtta
     1621 tcgatttctt gaatattacc agcaccacca agtccttgca agattagttt gccaagttcg
     1681 ttttctgtca tttcttcgtc ctgatcttcc aacacatcat cgttggatcg accaggagtt
     1741 ttcacatcaa atcgctcaat gacatactta aatacaaagt aatatgcaac tgcataaaca
     1801 aggcccaatg ctacaactaa gtaccaacga gtataagtac cttgtaaaat tccgaagata
     1861 gccaaatcaa taaatccacc accggcattc ccaactgcga ctccgaatag attcattaac
     1921 ataaaggata aaccagccat gaatgcatgg aagacccata gaatcggtga gataaagata
     1981 aaggcgaatt caattggctc agtaatcccg gttacaaaac aagtagccgc tgctgcaatc
     2041 aacatccctt taatctttgg acgttgcgcc aaatatgaag ttttgtacat tgcatagcat
     2101 gctgccggca atccgaagac cataaatgga tgagtacctt gtgtcaagaa gcgagtagca
     2161 cttctcaaaa tatccatgtc ttttactggc aatgctaatt cagtattgaa gatggttaat
     2221 gctccagaaa ctgtttcacc attgatagtt gccgtaccgc cgattggtgt aaatcgaatc
     2281 aactggttta gtacatggtg aagacctgtt ggaatcagca ggcgctctga gaagccatat
     2341 aaaaaagttc caattacgcc tgacttacca atcaaaccgc ctaaagcatt gatagctgta
     2401 ttgacaaaag gccagatgaa aaatgaagcg attgagatga ttggaatagt taggatgatc
     2461 ataattggaa caaaacgatt gccactaaag aaactgaaag ctgctggtaa ttgttttgtg
     2521 tgatagcgat tgtggatcat ggctgtccat aatcctgcaa tgataccgcc gaaaacaccc
     2581 atacgatatg tgaatgtgcc aagtaaagtt tcatacatat tggaagtttc tactgctttg
     2641 atttgactca ttccgtcaga catcaatgtt tcaacagaag tgctttccgc tgtgatgcct
     2701 ctaagctgaa gcagataatt gataactaca tggaacagaa taaatgcaat aaccactgaa
     2761 aatgctgcaa tttccttatc ttctctagac atcccgattg ctactgaaat agcaaataga
     2821 ataggaagta gtccaaacat cgtaccaaca atagcctgta ctccaccaac aaatccttgg
     2881 atcacggttg cattagcgaa gctttctccc accaaagtag gattttgtaa aattgaaacg
     2941 attcctagta gtagtcccga tgcgacaata atagacaacg gacccattaa tgatttccct
     3001 aacttctgga tataagttct caatataaac accttcttct ttttagttga tataaacgtt
     3061 tacatccctt gtacaacgaa agtatagcac cctgaaaaag cgctaacaat acgataggtc
     3121 tgataaaaat caacattcag cacttggttg ttcattcaaa acgtgaatct tcaatcacag
     3181 aaaaatacgc gctatcaagc acgtatttta ggtgtaaata tatgcatcaa ctttattaat
     3241 caacgctact ttgtttgtaa tagttaatat acccttcata cagtaagcta agagcaagat
     3301 tcaaggtgca gaatgatgag ttgttcaact cattcacatc attaatgttg gtcacttgat
     3361 agtataggct atagtctgac agactggcta agttattttg accaaatcgt gtaactgaga
     3421 ccaactgtgt ccctttcata tggattcttc ttaaagtatc atcaatccga tcaacacgtc
     3481 cggacaatga tatgacaaat agtaaatcat tcgaggtcaa agcttttgaa atcaggtcta
     3541 attcattctt ttccggtaca acgataatgt agattcccaa gttccacatg cagcgtgcaa
     3601 actcattgag catcattctt tgtccatatc ctgttgcgta ggcaaatatc ttatctgcct
     3661 ttctaatcgt ttcatatata tcctcaacac ttttatcatc ttcaaacaat ttggttgtcg
     3721 cttgaacatc tccgagtaat acttccggta aaaacaaact acttttatcg actctggttt
     3781 tttgcatgcg tgcaaattcc tctttaaggt aatatcgaaa ttcgccatat cctttgaatc
     3841 cgagtttctt agttagtcta ataatcgttg agtcagacac atttgcttgt tttgctaatt
     3901 ccgatacagt ttgactgatg ctgatttcca tattttgagt gataaaactt aatacataat
     3961 catcattctc attcaattta ctgtgatgtt catttactaa ttgctccaat gtcataatca
     4021 gacctccaaa aataataaaa aagattgctc tctcatttta atacgaaagc acaatctttt
     4081 taagtactat taaattttga aaagaagttc tccttcagaa accttttttt cagagacttc
     4141 tagtttaata cccttatctt cgggaaatag aaccataaca acatctgaca gtttttccgc
     4201 cttgatttgg tcacaatcaa aaatcaccaa tgactcgccg gatgatactc gttgaccttc
     4261 ttcaacgagc acttgaaacc cgactccctt taactgaaca gtatcaattc caatatgaat
     4321 caataaatct ctaccttgat tactagtcaa tgtgagtgca tgctttgttg gaaatatcga
     4381 tttaatgaca ccgtcaatcg gtgctaccac cttagcgttt gttggttcaa cagcgaatcc
     4441 ttcgcccatc atcccttgcg aaaatacagg atcgcttact tggctaaggg gaatcagatt
     4501 tcctgatact ggagcaacta atttttcttt tcgactaaaa aatcccattg acttactcct
     4561 taatcttttg aaatgtccat cacaaggttt aaaagaacat ccgccccttt tttgacatcc
     4621 tcaaaatcag tccactcatc aggatgatgt gtaatgccac ctttggatgg aatataaatc
     4681 atatttgttg ggacaaagtc ggtcatcatc atcgcatcat gtccagtacc attgttaatg
     4741 acttgatagc tatagcccaa ctgatcgcaa atatgtttca tcttctcaac attaccttga
     4801 tccataacac ttggatttgt aggattactg tagttaattc gagccatttc ctccagctca
     4861 gttctaactc cagtttcccc ttgaatcact gataactcat tcaacaactt ttctttaatc
     4921 tcttgaggat tagttgtctt tgtatcaaag gttctgattt cgattaaagc ttcgacaaag
     4981 tcagggacaa attgatttga gttcggtttg attgtaagtt taccgacggt tccggcaatc
     5041 ccttcaccaa gattcttcat ccactgttca acaaaaagaa caaatttaga agcagcaact
     5101 aaagcatctt tccgtttatt cattggagca gtcgagtcct cagtttctcc ataaaaacgt
     5161 gctttaaaac ggccgatgcc aggaataaat tcaatcaatc cgatatcttt gccttcatct
     5221 tccaggactg gtccttgttc gatatgaagt tcaataaaat ttttgattgt ttttggatct
     5281 cgcttagcgg cctctaaatc tggttccact ccataagcac gaagagcttc caattttgtt
     5341 tgtccatggc gattttttac tgtttccaac tcactcatgg ttaatgttcc catcatagct
     5401 cgtgaatttg agacacccgt acttggccca aatgtctcgc cctcctctgc attcatcaga
     5461 attaggtcaa ttgggtaatc attttcaaaa ttattttctt ccagaatccg cattgtttcc
     5521 agtgcagtaa caacgccagc cactccgtca aatctgcctc cattaacaac tgaatcataa
     5581 tgagagccaa tcatcactga tggtgcatca gcctttttac cggctctacg accaaataat
     5641 gtagagaagc cgtcttccca aatctctaaa cccagttgtt ccatttgatt gatcaaataa
     5701 tttcttgctt cacgatcttc tttagagtaa gcagagcggg tcactccttc acctggagtt
     5761 gcattgtact cggctaaatt ttcgatatcc ttttttatac gctcaaaatt tgtcttcata
     5821 attaaatcct ccatttccta caagtgaatg ttagcaccca tagaaagcgg ctacaatata
     5881 atcaatgaag attgaatcga tattcgaaaa ctgaatattg cttcggttaa tgacttagaa
     5941 atcattatcg tagattccac cataagaact ttttctcgtc tcataacaca actcgagagt
     6001 ggattggttt aaaaaactct cgataaatgc gtccacttta ttgacacaga tccatggatc
     6061 gatcgcacct tgaaatttaa acccagaaat agtcccatca tcttctatac cgattaccag
     6121 cttacctcca tcggcatttg caaacgctac tatgtgtttt ataatatctc taggcttaat
     6181 ccgtgcactt tttctatcaa aatactgatc ttctttttca cttataaact tttgaatctc
     6241 ataatccatt taaaatttcc cttcttaaat ttttttatta tagagccgta tattcaattt
     6301 ccacaaaaaa ataatctaaa acaaaacaga caccttcatt ttgatatgtt ctcccacata
     6361 tgaatcggtc gctatacagc attacatata gtaattattc atactgcgaa ataaatagct
     6421 ccaaatcttt agctggcgta gttaagctta aattcgaaat gaaagtttca ttgactattc
     6481 cactttcatc aaatcccttc ttcaaccaat cagagttcca caacgtaaat cctttaggat
     6541 tagaaacttt cttacataat tttgcactat aggatttctc tcttgaatga attaaagaaa
     6601 gtctttgtaa ccaaatttca actatatccg tattaggagt attaacaaat tttcttaaga
     6661 ttttatccaa gtataagttg atttcttcta cagcaataaa ctcaaaaagc ttacctaaaa
     6721 tcacaataca agcctccaaa gttctaggat tcttttgcat tatattaaca agaatactaa
     6781 tcaattggta gatatcatct ggtcttttgt ttaaagtaga aactctatgt gtatagactt
     6841 cacttagcgc tttacttaaa ctcccgcaat ttggatattt gtctcctaaa agctttacct
     6901 gtagcaaatg cttttgaagg ctaagcttaa acttccactt attattactt ttctctgtaa
     6961 aacttgcatg tttaagatcc cagtaaattt tatcaggctt aattgcatct aaaattatat
     7021 tatccgataa gaatgtttta tttgaattta gcttcaaatt tagatccgat aatacttcag
     7081 ataacaatct gataatcact tcagcttgat cttttcgaac cgaaaaaatt ctgtaatcat
     7141 ctctgtaacg aagtattttg aactctttaa tctctgtttt ttctatcttg ttcagtaaca
     7201 ttttatcagc atacccaagt actatttcag ctacaaaatc catcaataca tttccttgag
     7261 gtataccgtt cgtttgtcca ttttgtaaat atcgtatttt agaatctatt aagtttccaa
     7321 cgccttgagt atcttttcta tgtgtcttag cccattcttt tgaatgtact gcccacggga
     7381 tagtatgtgt gtacattgag ctataacaat cggtaatatc tgaatgaatg cagtagtcat
     7441 actccaaagc ataactaatc tgtgcttgtt caaggttctc ccaccaattt aaaatagttt
     7501 ccgcagtatc gctctttgaa gaaatagatt ccacaggaag tgatatacat tcaattcgct
     7561 tgtcggcttg gaattcttca aacttatttt tgatgtgatt ccaattattt ttctgtgtaa
     7621 tcccgtttac taagtctaca tatactaatg gatgtaatat ttgaaggggt ctccaagcat
     7681 aattaccatc tttattagct aatattcgat gattaacatc aactgcctca gagagcgatc
     7741 tcttatgtct aactagttca gtaaatggtt tgttttttaa taaatccact gctttctcaa
     7801 tgcctactga taaatcaata tagttgggca acgatgtact aaaaaaactt gatgttttta
     7861 aaaagaattg ctttgcttct tgatgattca tctctaaaat actttttcta gtcctagtca
     7921 aattcaaaat ctcctagatc aattaagttg taatgtaaga ggatacccta aggttacaca
     7981 ccctatttac tattatacca gtattcattc gtgatacata agggtgtaac attaagaact
     8041 ctatctaaaa gactacaagt agtcaccttt aattgtgagt aattttcttt atccaatcct
     8101 tgtgcaacct tgtcgtcacc ccttgtatat ccccgtaccc cggatgtttc ggaatatcaa
     8161 aaatgtgctc aaattcctga ttttcatcag tctcccactc atgattcaac ggttcatgcc
     8221 actctataaa ccttgaccat aaaatgttct ttctataaaa ctcaatagga atttcaaaac
     8281 agtaaaaatt gtatttgcta aaatctgtaa aattatcttt acgagcatac tttaaatggc
     8341 aggtatctcg ccacaaccaa attaaatctc gatccaatgg taactgaatt cgtttcgtat
     8401 actcttcttc aagccaatga agtgcaccaa ctgcattcgg attatcgaaa aagacagata
     8461 attcagtata gcgtttcttt ggataaataa atccttctga atcaacagat tgcaaaaaat
     8521 ctactggttg aaccgtgtac actttcattt attcgccacc tttttttgac tacttatagt
     8581 catttaccaa tttgaaatct gcatactttt tcattcaatt gagcccagca ttaattgtac
     8641 acgagttttc ttcactaacg gatagtatca ggatatacta atactatcct gaatagcgaa
     8701 atgaggtagc caaaatgaga aaagtcgatg aatatatcaa aaagaaatcc caaaaaagta
     8761 aagtatggca aagaacattt gagttagagt ataagaaact ctcagatagc ctcaaaaagg
     8821 acacttataa tccaaatgaa caaagtaaag cagataacca ataaagatta ccctcccata
     8881 tttaacattg cttcacgaat cagtaatcaa tgactacaag tagtcattga agcaaatctt
     8941 cataagatac ctgtagaata tccctaatag ctctcaattg atccagctta atatgttgcc
     9001 ggccaccttc aatcttaacc aatgtttctc ttgtcatagc gattttactg agctggagtt
     9061 ctcgcaccag ttctgtctgt cccattccct tttctaatcg gattcgtctg atattgctac
     9121 cgattacatt ttctttatct attaactttt cttccttcaa aaatagcacg ccttttggac
     9181 taaaattagt cctttttctt attaatctta aagatttggt atgctacaat gggactagta
     9241 ttagtccata ttcaatttta aggaggaatt tttttgaaga aatttgcaat cattctaacc
     9301 tgtgctttac tcacgggagc tataagtggg tgcgccactt ctgacacaaa ttctacaagt
     9361 actgaaacaa gtacaacaac ctcagaaaag aattatccta tacatttttt tccagttcca
     9421 gatggatttg aagcttatta tcatgataaa aaactagatg ttccattgtc agaaagtaac
     9481 tctattgaaa aagttttgcc aattgaaatt gaaaaaaatc aagagaatat aaaactcaag
     9541 gccgagcttc ccatactgaa cagcacagaa attgattggc acgagaatat taaagtaact
     9601 attgaaaaag gagcgttagc acaactttat actgaaaaag ttgaacaatt ttttgagtct
     9661 ggtgccaata aagacttttc aaaaataagc gattattcgg ataacttttt taatgagacc
     9721 caagatgaaa tcacgaactc aacttttatg gctgaagata tgtacaatat gacgttaaaa
     9781 aaaataaaag cttacgacca tccattaaat ctgacactaa ataatggatt actctctttt
     9841 gtattagcag gttacgttga ctatgaatat tcaccagatt atttggagac acctcttcaa
     9901 cacgagaaca atgatattaa ttttagattt gtctacgagt caaaaagtaa aacatggtta
     9961 gttgatcaat taagcactat aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa
    10021 gctgatatgg tcattaaaat cttctaaaaa agaagcgttg aaggagaaat attcctttca
    10081 acgctctttt ttatatgctt agaaacataa ataaattgta tactttcttt ttagccccta
    10141 ttacttttca cgaaaaatat cttttttcga cactagcgtt tctctactta taatattgta
    10201 cacagttttt gtattattga aaaattactc attatttagc attaatccac atcgttttat
    10261 caatcgacct tcatactaaa ctactttaaa gtcatcttcc ttctcatttt tcattaaatc
    10321 agatacataa gcccatgtac ccgttcgttg taaataaagt tcttcttgta gcagctccca
    10381 ctttctctta gaaatcataa tgacgccatc gttcaatcca tttttagaat ccctagtaat
    10441 ttctatttca gaatttgaat gaactacttc ttgcaacact ttacctatat tttttttgaa
    10501 gtttgttgga gtaatagtat ccaaaatgaa cacctctctt ctctatacct ctattatgaa
    10561 gcgcccatca aataaagcaa gcgttgagga ataattcttt caacgcttgc tttattaagc
    10621 tttcattttg tagtttgact aataccaata atctgatctc taaacaatcg aaaaacttct
    10681 tgtggttgat aataatctgt atctatttca aatgtttctg agaacaattc attgaatttt
    10741 tctactgaaa tgatgatttt accattctcc tctcgactgt tttctattat ttctgcttct
    10801 aattcaaata aatctagtga cgaactaaaa tcataaatcc tgtcatattg aacaaaaaaa
    10861 tcaagaaaaa gttgctcaaa ctcttcctta tttatcacta gtgtttcaag cggaacaact
    10921 ttattttcat cagccattct tccaatttcc tcactttcat tttcaaaaaa tcgtaaaatt
    10981 gatatagatt ctttgtctct aatcactcgt gatattatct tgacagtcct acatctaatt
    11041 caattaaagt ataagccact ttcaaataaa agattacaaa gtaaatggca agccaacaaa
    11101 ataaccatct gcttcacgta ttacgatttg ataattctgg atttcgctcg gaagttcata
    11161 ataataatta acagtttctg ctttgccact ttcaatatat tctggattat tcaatatcat
    11221 agcatactca taaagttctt caccattttt att
//
LOCUS       DACCDF010000086        11096 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCDF010000086 DACCDF010000000
VERSION     DACCDF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.090"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(390..1337)
                     /locus_tag="IVK51_002317"
     CDS             complement(390..1337)
                     /locus_tag="IVK51_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAP6431880.1"
                     /translation="MHLEFSDYESLLVKICEDYYLEDINISEISKKYGITRYKALKYI
                     EDAREKQLVSISINSPYARNYELEEIFKNYFKTNIYILKSSEDLSQHDLLFSKFAADY
                     IQELIKKANVVALSWGDTVYKVIDQFKMGAREELIFTQFIGEIGKYNSLAGSTRLVQK
                     AAERYESSYLTLSIPLYVINETARELMALEPSLANTLATASHADMLISGIGTLSSIDS
                     VEPWNKHRKILFPSMTDTVGFLYGRPFNINGEFIKASEDKTFGLSLKEIFDIPKRIGI
                     CKSKFKAESCLGALNGNFFTDLFLDEKTAWKILSLIKKE"
     gene            1525..2712
                     /locus_tag="IVK51_002318"
     CDS             1525..2712
                     /locus_tag="IVK51_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HAP6431881.1"
                     /translation="MLDEVLEIHKKNVGVLRMETTEAVQDDQDLAKMYTPGVAELSLM
                     IEKNHALARELTISGKLVAVITDGSAVLGLGNVGNQAGLPIVEGKALLYKNLAGVNAI
                     PLAIEQKSVDEIVQTIVNLQNSFAGIHLEDIAAPKCFEIEEKLQEKLSIPVYHDDQEG
                     TAIVVLAGLINAAKIQRKTLTELRVLINGMGASGVATARLLIAAGIKNLTLVDKQGRL
                     KKQDSTLNKYQRELLDHIHDTNNNASVLEDAIVGQDVFIGLSIGNVLTPEMIGKMNDN
                     PIIFALANPVPEITPDLAKESGAKLIATGSSKYPNQVNNILVFPGLFKGLLLSKANKV
                     DLSLQLEVAKQLAAKVAEPTVEKFIPNVFDEGVANVVSQAVLDYLQKLKENEDNIHAE
                     NIK"
     gene            2693..3997
                     /locus_tag="IVK51_002319"
     CDS             2693..3997
                     /locus_tag="IVK51_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP6431882.1"
                     /translation="MLKTLNRIKIGFIPLSLYIILVLAYLAMTISNIVPENDMLVSLG
                     IMTVFAYLLEEIGKTIPILKDLGGKVLVVTFLPAFLVYKHWLPQSTVTVVSDFMNNNN
                     FLMFFITLLVVGSIASMNRQTLVKATSRIIIILILCDVIGSLIGTGVGILLGLSAWNA
                     YFFIVAPIMAGGVGEGAMPLSIGYAAILSGMGQNDIFGKILPCVMLGSLVAVLLSGML
                     KKIGEKYPQLNGQGNLIDGDDEHLENLKGSAKPVNIEKTLLAGVVAITIYFVAVYVEH
                     LLKSLLGFSIPSPVILLVSLMGAKMLGFIPKNIEEGGNSLYGFTVRGITPPLLFGVGV
                     AKTDWLELIEVFKNPATIVTIIVTVVAIVATAFIAAKFLKMYPVDTAIAVSCCSGQGG
                     TGALAILAAGDRMELMPFAQVAVRLGGAMTVTFAIFLMGLLS"
     gene            4023..5036
                     /gene="citC"
                     /locus_tag="IVK51_002320"
     CDS             4023..5036
                     /gene="citC"
                     /locus_tag="IVK51_002320"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAP6431883.1"
                     /translation="MFDQTDIQRLFLKNNYIKDQWENLLLDSGIQKKQIEDFQHLDQT
                     LGIYHKEKLVGTVSYQNNVIKYIAVSEKYKDGGHTFNVLVSAIIQAMSQQEIFHYIVF
                     TKPRYQKSFNFLGFETIVSTPKIAVLEMGDSSIKQYLERIPKVNGEKKAAIVMNANPF
                     TEGHYYLVKQAANNNDWVYVFVLEKEQNLFSTKDRLKLVQQGLQKLKNVIVLSGGEYI
                     ISPATFPTYFLREDDEIAKEQMVVDATLFKERIATYLDISARYVGEEPLSPMTRSYND
                     ILETILPPEIKVHVLARKKTEQHQVISASQVRKAYLAGQLEKIKYMVPETTYQYLKNK
                     RER"
     gene            5040..5333
                     /gene="citD"
                     /locus_tag="IVK51_002321"
     CDS             5040..5333
                     /gene="citD"
                     /locus_tag="IVK51_002321"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAP6431884.1"
                     /translation="MEINKSAIAGTLESSDVQIMISPNTEGIEIDLDSQVIEQFGKQI
                     KKVIYDTLAVYQIKNVKLKVVDKGALDCVIVARLKTAIHRSLGIQHQETPWEV"
     gene            5338..6243
                     /gene="citE"
                     /locus_tag="IVK51_002322"
     CDS             5338..6243
                     /gene="citE"
                     /locus_tag="IVK51_002322"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAP6431885.1"
                     /translation="MMKERLRRTMMFVPGNNPAMIKDAGIYGADSIMFDLEDAVSMAE
                     KDSARMLVAEALKTVDYGDTELIVRINDLHSEFGRADIFAMVKAGAHVLRLPKTETSE
                     DITEVEKVIIEAENYHNVPIGRTLMMAAIEGASGVLNAPAIAKASDRLIGIAIGAEDY
                     VTNMKTKRYPDGNELFFARSMIVHAARAAGISAFDTVYSDVDDMEGFLNEVRLIHQLG
                     FDGKSCINPRQIPVVNTVYQPSIKEIKRSLDIISAIEEAKEKGSGVISLNGKMIDKPI
                     VERAQRTLMLAKASRLIDEEGNIHA"
     gene            6236..7795
                     /gene="citF"
                     /locus_tag="IVK51_002323"
     CDS             6236..7795
                     /gene="citF"
                     /locus_tag="IVK51_002323"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAP6431886.1"
                     /translation="MHNKQRRSIPSSVLTKYKKEFQSVSLSPQTIQRCAPKVTTVTNQ
                     TNKLMHSIKEAIEKSGLKDGMTISFHHHLREGDFVFNQVMAVIREMGIKELTLAPSSL
                     TNTMNEMVIECIKDGTVTHITSSGMRGSLGEFISHGGMDNPVILRSHGNRPRAIESGE
                     LKIDVAFLGVPTSDDYGNANATVGKSVFGSLGYAKVDAQYADKVILLTDHLVPYPNTP
                     ISIPQTQVDYVVEVEAIGDPNKIGAGATRFTKNPKELKIAEMVKDVIVHSPYFKDGFS
                     FQTGTGGAALAVTRYLASEMEEKQIKASFALGGITKPTVDLLEKGLVEKVLDVQDFDK
                     GAAEGMARLENQQEIDASFYADPSNKGAVVNQLDVAILSALEIDTNFNVNVMTGSDGV
                     LRGAIGGHQDAATAKLTIISAPLVRGRIPTVVNDVTTLITPGKSIDVLVTEVGVAINP
                     QRKDLIAIFERIPQIPVFTIEELQQKAEKIVGIPEPLQFTDRIVAYVEYRDGSILDVV
                     RQVKEFEEERS"
     gene            7792..8349
                     /gene="citX"
                     /locus_tag="IVK51_002324"
     CDS             7792..8349
                     /gene="citX"
                     /locus_tag="IVK51_002324"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAP6431887.1"
                     /translation="MMFDTGEIQEIEDILIHKEQRANLQSNLLSKYSNSSLMVFTLNI
                     PGPIKNNPMIEQVFRQGQQSIQQMFLKKKVEMTYTKEINLPSGLDWYVIVTEAPLNLK
                     KWLVTLEEQDALGRLFDLDVLFKKGEQITIVSRQALNHGERPCFICGKSAKVCARNRT
                     HTVKEMQKKIEQIVADSLKGALKHA"
     gene            8342..9205
                     /gene="citG"
                     /locus_tag="IVK51_002325"
     CDS             8342..9205
                     /gene="citG"
                     /locus_tag="IVK51_002325"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAP6431888.1"
                     /translation="MHDNIQKIAQEAQLAMLYEVTCINKPGLVDPVDPGSHQDMDIFT
                     FLQSSVVLTPYFEEFIQTGLDRQHQPIEETFMAIRQIGLEAETAMFSVTNGINTHKGA
                     IFSLGIFLAICGRLTIWKVPCKKSFFQKEIQKMTKNLLNDFGKIDQTKPKAWTWGEYL
                     FVNHGLTGIRGEAKRGYPCVFDRGLPYYQAYQGSQQDKMIDTLLFLSMEVEDTNLIKR
                     SNNRHVFEEYQLLIRPYFELGGVKTIQGKKYLDYLNQQFKKKNWSIGGSADLLILTIF
                     LDKIMDKGWLC"
     gene            9439..9848
                     /gene="tnpA"
                     /locus_tag="IVK51_002326"
                     /pseudo
     CDS             9439..9848
                     /gene="tnpA"
                     /locus_tag="IVK51_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10485..>11096)
                     /locus_tag="IVK51_002327"
     CDS             complement(10485..>11096)
                     /locus_tag="IVK51_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6431889.1"
                     /translation="IGITKDGDREIIGFMIQSGESEETWITFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINL
                     AREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV
                     RRREKIIRIFPNCVSANRLIGAVLIDQHDEWLSSSRKYIKFAK"
BASE COUNT         3857 a         1730 c         2176 g         3333 t
ORIGIN      
        1 cgtggagaaa taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc
       61 aaagggtggg gaatgaataa taaagttaga tagaaccccc tttttatagc ataaaaaaga
      121 tttagaagga tatatgatcc tattgcatga gaagtacaat aaaattaata agaagtaaca
      181 ttaacgaaaa aacgtggaaa ttattcagtg aatacaaaga aattaaatct tgaaaagtaa
      241 tggagtatgg cttggaaaag ttcaaaaaat atcaagacaa gcttctcaaa aaaagggatt
      301 ttgatatcca ataaaaagag tgaatcattg tactgccctc caaaaaagta gtaccaaaaa
      361 tctaaatttt ttggaggatt ttacatgact cactcttttt ttatcaaaga aagtattttc
      421 caagcagttt tttcatccaa aaataaatct gtaaaaaaat taccattcaa ggcgcctaaa
      481 caactctccg ctttgaactt acttttacaa ataccaattc gttttggaat atcgaaaatt
      541 tcttttaaag aaagaccaaa agtcttatcc tcagatgctt tgatgaattc accattgata
      601 ttgaagggtc ttccgtaaag aaatccaaca gtatccgtca tgcttggaaa taggatttta
      661 cgatgcttgt tccagggttc gacagaatca atagaggata aggtgccaat tccagaaatt
      721 aacatatctg catgcgaagc ggttgctaag gtattagcta aagaaggttc tagagccatt
      781 agttcacgtg cagtttcatt gatgacatat aaaggaatac tgagagtcaa ataagaagac
      841 tcataacgtt cagcagcttt ttgaactaat cgtgtggagc cagctaagga attatatttt
      901 ccgatttcgc caatgaattg agtaaaaatt aattcttcac gagcacccat tttaaactga
      961 tcaatcactt tgtaaacggt atctccccaa gatagtgcaa ccacattggc tttcttaatt
     1021 aattcttgaa tgtaatcggc agcaaatttt gaaaataata aatcgtgttg tgataagtct
     1081 tcactacttt ttaatatata gatattcgtt ttaaagtaat ttttgaagat ttcttctagc
     1141 tcataattcc tagcatacgg agaattaatt gaaatactaa ctaattgttt ttcacgtgca
     1201 tcttcaatat attttaaagc tttatatctg gtgatcccat attttttaga aatttcacta
     1261 atattgatat cctcgagata atagtcttca caaattttta caaggagtga ttcatagtca
     1321 gagaattcca gatgcatagt atagctccta actttttcaa atagatattt tttctcatta
     1381 taccaaaaaa attgaattta gtcttttttt aaatttttaa aaataagcat cattttttta
     1441 aaatttaaaa tgaaagggtt tttttatttt aaaaaaagtg ttaaagtggt tttgtaatta
     1501 aaaaatgtaa aaaggagtga aggtatgctt gatgaagttt tagaaatcca taaaaaaaat
     1561 gtaggtgttt tacggatgga aacaacagaa gccgtgcaag atgatcaaga tttagcaaaa
     1621 atgtacaccc caggtgtagc agaactaagt ttaatgattg agaaaaatca tgctttggca
     1681 agagaattaa caattagtgg taaattagtg gccgttatta cagacgggtc ggcggtatta
     1741 ggtcttggaa atgtaggaaa ccaagctgga ttaccaatcg tcgaagggaa agcgctgctt
     1801 tataaaaatt tagcaggtgt caatgcgatt ccattagcca tcgaacaaaa atcagtagac
     1861 gagattgtac aaacaattgt caatttgcaa aatagttttg cagggatcca tttagaagat
     1921 attgcagcac cgaagtgttt tgagattgaa gaaaaattac aagaaaaact ttctattcct
     1981 gtgtatcatg acgatcaaga aggaacagcg atagttgttt tagctggatt gatcaatgct
     2041 gctaagatcc aaaggaaaac attaactgaa cttcgagttt tgatcaacgg catgggagca
     2101 tctggggttg caacagctag actattaatc gctgctggta tcaaaaatct aacattggtg
     2161 gataaacaag ggagattaaa gaagcaagat agtacattaa ataaatatca aagagagttg
     2221 ttggatcata tacatgatac aaataataat gcaagcgttt tggaagatgc aatcgttgga
     2281 caagatgttt ttatcggtct ttcaatagga aatgttttaa cgcctgaaat gattggaaaa
     2341 atgaatgata atccgattat ttttgcttta gccaatccag taccagagat cacacccgat
     2401 ttagcaaaag aatctggtgc aaagctaatc gctacaggtt cttcgaaata tcctaaccaa
     2461 gtaaacaata ttttagtatt tcccggattg ttcaagggac tattactatc gaaagcgaat
     2521 aaagtcgatc tttcattaca acttgaagta gccaaacaat tagctgctaa ggtcgcagag
     2581 cctacggtag aaaaattcat ccctaatgtt tttgatgaag gagtcgcaaa cgtcgtttca
     2641 caggcagttt tggattattt acaaaaacta aaagaaaacg aggataatat acatgctgaa
     2701 aacattaaat agaattaaaa ttggttttat tcctttatct ctttatatca ttttggtact
     2761 cgcgtatctt gcaatgacga taagcaatat cgttccagaa aatgatatgt tagtttcttt
     2821 gggtattatg actgtttttg cttatttatt agaagaaatt ggtaaaacta ttcctatttt
     2881 aaaagattta ggtggtaaag tattagttgt tacatttttg cctgcatttc tagtctataa
     2941 acattggcta cctcaaagta ctgtaactgt ggtttctgat tttatgaata ataacaattt
     3001 cttgatgttc tttatcacac tactagttgt cggcagtatt gcttcaatga atcggcaaac
     3061 attggttaaa gcaacttcac gtattatcat tattttaatt ttatgcgatg tgattggttc
     3121 tttgatagga acaggtgtgg gaatactcct tgggctatct gcttggaatg cgtatttctt
     3181 tatcgttgcg ccgattatgg ctggtggtgt tggggaagga gcgatgccat tatcgattgg
     3241 ttatgcagca atattatctg gtatgggaca aaatgatatc tttgggaaga ttctaccgtg
     3301 tgtgatgttg ggaagtttag tcgcagttct actgtctggt atgctcaaaa aaattggaga
     3361 gaaataccca caattaaatg gtcagggtaa tttgatcgat ggggatgatg aacatttaga
     3421 gaacttgaag ggttcagcta aaccagtcaa tattgaaaaa acattgttag caggcgtggt
     3481 agcaataacg atttattttg ttgctgtcta tgttgagcac ttattaaaat cactccttgg
     3541 cttttctatt ccttcacctg tgatcttatt agtctctctt atgggggcta aaatgcttgg
     3601 ttttatccct aaaaatatcg aagaaggtgg aaattcgttg tatggattca ctgttcgtgg
     3661 gatcacacct cccttacttt ttggcgtagg ggttgcaaaa acagattggt tagagctgat
     3721 cgaagtattt aaaaatcctg caacgatagt aacaatcatt gtgacggtag tagctattgt
     3781 cgctacagct tttatcgccg caaaattttt aaaaatgtac ccagttgata ctgccatcgc
     3841 agtttcatgt tgtagcggac aaggcggtac aggtgcttta gctatcttag ctgcgggtga
     3901 tcgcatggaa ttaatgccat ttgcacaagt tgctgttcga ctagggggtg ctatgaccgt
     3961 aacgttcgct atctttttaa tgggtctgct atcataagaa cataaagaaa ggacgtgacc
     4021 atgtgtttga tcaaacagat atccaacgat tatttttaaa aaataactat atcaaagacc
     4081 aatgggaaaa tcttttgctt gattcaggaa tacaaaagaa gcaaatcgaa gattttcaac
     4141 atttagatca aacattgggt atataccata aagaaaagtt agtaggaaca gtatcttatc
     4201 aaaacaacgt gattaaatat atcgccgtgt ctgaaaaata taaagatggt ggacatacat
     4261 ttaatgtact agtctctgca ataattcagg caatgagtca acaagagatt tttcattata
     4321 ttgttttcac gaaaccgcgc tatcaaaaaa gttttaactt tttaggtttt gaaacaatcg
     4381 tatctacccc aaaaatagct gtattagaaa tgggagatag ttctataaaa cagtacctag
     4441 agaggattcc taaagtgaat ggtgaaaaaa aagcagcaat tgtcatgaat gcgaacccat
     4501 ttacagaagg gcattattat ttagtaaaac aagctgccaa taacaacgat tgggtatatg
     4561 tatttgtact cgaaaaagaa caaaatcttt tttcgacgaa ggaccgactc aaattagttc
     4621 aacaaggatt acaaaaactg aaaaatgtca ttgttctatc aggtggcgag tatattatta
     4681 gccctgcaac gtttcccacc tattttttac gagaagatga tgaaatagca aaggagcaaa
     4741 tggtcgtaga tgctacttta ttcaaagaaa gaattgccac ttatttagat atctctgcta
     4801 gatatgtggg agaagaacca ctttccccaa tgactcgttc atataacgac atactggaaa
     4861 cgattttacc ccctgaaata aaagttcatg tccttgcaag gaagaaaacg gaacaacacc
     4921 aagttatttc tgcttctcaa gttcgtaaag cgtatcttgc tgggcaattg gaaaaaataa
     4981 aatatatggt tccagaaaca acctatcaat acttaaaaaa taaaagggag cgttaagtaa
     5041 tggaaataaa taaatcagcg attgctggaa cattagagtc aagtgatgtc caaatcatga
     5101 tttcaccgaa tacagaagga atagaaatcg atttagacag tcaagtgatt gagcaatttg
     5161 gaaaacaaat caaaaaagtt atctatgaca cactagcagt ttatcagatt aaaaacgtaa
     5221 aactcaaggt agtagataaa ggggcattag attgcgttat tgttgcacga ttaaaaacag
     5281 cgatccatcg ttccttaggt atccaacacc aagaaacacc gtgggaggtt taattagatg
     5341 atgaaagaac gtttgagaag aacaatgatg tttgttccag gaaataatcc agcgatgatc
     5401 aaagatgcag gtatttatgg tgctgactcg attatgttcg acctggaaga tgcagtgtct
     5461 atggcagaaa aagacagcgc aagaatgctt gtggctgagg cattgaaaac agtggattat
     5521 ggtgacacag aattaattgt tcgaataaat gacctgcact cagaatttgg acgtgcagac
     5581 atttttgcaa tggttaaagc cggcgcgcat gtattacgtt tacctaaaac agaaacctct
     5641 gaagatataa cagaagtaga aaaagtgatt atagaagcag aaaactatca taatgttccg
     5701 atagggcgaa cattgatgat ggcagccatt gaaggtgctt ctggtgtatt gaacgcacct
     5761 gcgatcgcca aagcatctga tcgattgatt ggaatcgcca ttggggcaga agactatgtg
     5821 acaaatatga aaaccaaacg atatccagat ggaaacgaat tgttttttgc tcgcagtatg
     5881 atcgttcatg ctgcacgtgc agcaggcatt tccgcatttg acactgttta ctctgatgtg
     5941 gatgatatgg aaggattttt aaacgaagta cgtttaatcc atcaattagg ttttgatgga
     6001 aaatcttgta tcaatccacg gcaaattcca gtagtcaata ctgtttatca gccgtcaatc
     6061 aaagaaatca aacgatcact agacatcatc tcggcaatcg aagaagcaaa agaaaaaggt
     6121 tctggcgtaa tttctttaaa tggaaaaatg atcgataaac caattgtaga acgtgcacaa
     6181 cggacattga tgttagcaaa agcatcacga ttgattgatg aggagggaaa tatccatgca
     6241 taataaacaa agacgtagca ttccatcaag tgttttaaca aagtataaaa aagaattcca
     6301 aagtgtttca ttatcgccac aaacgattca acgttgtgca ccaaaagtaa cgactgtaac
     6361 taatcagaca aataaattga tgcactcgat caaagaagct atagaaaaat cgggcttaaa
     6421 agatggaatg acgatttcat ttcatcatca tttgagagaa ggtgattttg tttttaacca
     6481 agtgatggcc gtgatccgtg aaatgggcat caaagaattg accttagcac caagttcttt
     6541 aacgaacact atgaatgaga tggttattga atgcatcaag gacggtacag taactcatat
     6601 cacaagcagt gggatgcgcg gcagcttagg tgaatttatt tctcatggag gaatggacaa
     6661 tcctgtgatt ttacgttctc atggtaatcg tccacgtgca attgaaagtg gtgaattgaa
     6721 aatcgatgtt gcatttctag gagtaccaac atctgatgat tatggaaatg ctaatgcgac
     6781 tgttggaaaa tccgtttttg gttcattagg gtatgcaaaa gtagatgcac aatatgcaga
     6841 taaagtcatt cttctaactg atcatttagt tccttaccct aatacgccca ttagtattcc
     6901 gcaaacacaa gtcgactatg tcgtggaagt agaagcaatt ggtgatccga ataaaatcgg
     6961 tgctggtgct acacggttta ctaaaaaccc aaaagaatta aaaattgctg aaatggtcaa
     7021 agatgtcatt gttcactcac cttatttcaa agatggcttt tcttttcaaa caggaacggg
     7081 tggtgcagct ttagcagtaa cgcgttactt agctagtgaa atggaagaaa aacagatcaa
     7141 agcttctttt gcattaggtg gcatcactaa accaacggtt gatttattgg aaaaaggtct
     7201 tgttgaaaaa gtgttagatg tccaagactt tgacaaagga gcagctgaag gaatggcgcg
     7261 gcttgagaac caacaggaga tcgatgcttc cttttatgct gatccaagta ataaaggagc
     7321 ggttgttaac caattagatg tcgctatttt atctgcatta gaaattgaca caaacttcaa
     7381 tgtaaatgtt atgactggtt cagatggtgt gctaagaggt gcgattggtg gacatcaaga
     7441 tgcagcaacg gctaaactta ctatcatctc agcgccgtta gtacgtggcc gaatacctac
     7501 agtagtaaat gatgttacta cattgatcac accagggaaa agtatcgatg tattagtcac
     7561 agaagtaggg gttgctatca atccccaacg taaagatcta atagctattt ttgaaagaat
     7621 tccacaaatt cctgttttta caatagaaga attacagcaa aaagcagaaa aaatagtagg
     7681 catcccggaa cctttgcaat tcacagatcg gatagttgcc tatgtagaat accgagatgg
     7741 ttcaatttta gatgtcgttc gtcaggtgaa agaatttgaa gaagagagat catgatgttt
     7801 gatactggag aaattcaaga aatcgaggat attttgatcc ataaagaaca acgtgctaac
     7861 ttgcaaagca acctgctttc caagtattca aatagttcat tgatggtctt tacattgaat
     7921 attcctgggc cgatcaaaaa taatccaatg attgagcagg tatttcgtca gggacagcag
     7981 tcaattcagc aaatgttttt aaaaaaaaag gtggaaatga cgtacacaaa agaaatcaat
     8041 ttgccaagtg gattggactg gtacgtaata gtaacagaag cacctttgaa tctcaaaaaa
     8101 tggttggtca cgttagaaga gcaagacgca ttaggtagac tttttgattt agatgtcttg
     8161 ttcaaaaaag gagagcagat aacaattgtc agtcgccaag ctcttaatca tggggaacgt
     8221 ccttgtttta tttgtgggaa atcagcaaag gtatgtgcac gtaatcgtac acatactgtg
     8281 aaagaaatgc aaaagaaaat cgaacaaata gtagccgatt ccttaaaagg agcattgaaa
     8341 catgcatgat aatattcaaa aaatagcaca agaagcacaa ttagcgatgc tttatgaagt
     8401 tacatgtatc aataaaccag gattggttga tccagtagac cccgggtctc atcaagatat
     8461 ggacattttt acttttctac aaagtagtgt agttttgact ccctattttg aagaatttat
     8521 ccaaacagga ttggaccgac aacatcaacc aattgaagaa acctttatgg cgattcgaca
     8581 gattggttta gaggctgaaa ctgcgatgtt ctcagtgaca aatggtataa atacacataa
     8641 aggtgctatc ttttcattgg ggatttttct tgctatttgt ggccggttaa ctatttggaa
     8701 agttccttgt aaaaaaagtt tctttcaaaa agaaattcaa aaaatgacga aaaatctact
     8761 taacgatttt gggaagattg atcaaacaaa accaaaagca tggacttggg gagaatatct
     8821 atttgttaac catggattga cagggatacg aggggaagcc aaaagaggtt atccttgtgt
     8881 ttttgaccga ggattgcctt attatcaagc atatcaaggg tcgcaacaag ataagatgat
     8941 agacacttta ctttttctat caatggaagt agaagatacc aatttgatca aacgaagtaa
     9001 taatagacat gtttttgaag agtatcaatt acttattcgt ccttattttg aattaggggg
     9061 tgtaaaaacc atacaaggta aaaaatattt ggattaccta aatcagcaat ttaaaaagaa
     9121 aaattggagt attggtggaa gtgcagactt attgatcctt acaatctttt tagataaaat
     9181 catggacaaa ggatggttat gttgatacta taatacatta ttttgtatta gtaagctaat
     9241 aactaaaaaa tggttcaata caaatatttt ataaaaaatg gtagtgccta tcctttcaga
     9301 ttgtagacaa agaaaaattt ctttgtctac aatttttttg ttaatgactt cagtcagttt
     9361 cgtgcgcaaa gcgtgcgaaa caagaaaacc atgcgtttct acacgtggaa gaactagcat
     9421 atcgaaagga gttttttcat gtcaaatgac gataaaagtt tagcccatac tcgttggaat
     9481 tttaagtatc atattgtatt tacaccaaaa tatcgacgaa aagtaatcta taggcaatta
     9541 cgaaaagata ttggaaaaat acttaggaag ttgtgcaaaa taaaaaacgt ggaaattata
     9601 gaagcacatg caacgccaga tcatattcac atgcttgttc aagtaccatc aaaaataagt
     9661 atatccagtt ttatagttat ctgaaagatc gtagtgctgt gctaatccac gagcagcacg
     9721 taaatcacaa atatagatac ggaaataaat cattttggtc ggaggaatat tatgtgagta
     9781 cagtcgaatt gaatcaaaag acaattgaaa aatacatccg tgaacaagaa gctgatgatc
     9841 gcatttgaga tagtataagt aaaagagagt agatgttgca ataacaaaag ggtaaaaatg
     9901 gactacctcc agtaaaatac cgaaaaatag tttaactttt ggagataagt ccatttaagg
     9961 gtctatagaa tgacaatttt tttaggggat tttactatta ttacttcaag acccgcttgg
    10021 attcgcaaag tgagtgaact cttaaaacta cctgatagta ggttgtttta agagttatct
    10081 gaaagtaaaa attgcattta tatttttgat aaatacgtaa atttatatat acgtatttat
    10141 caaagtttta ggccaaagga atattgtgta aatacactag gattgaatga atagaccaga
    10201 agaaattgcg tccaagaaca aaagaagcat gatagtgctc tatataaatt gggcattcaa
    10261 gaatctgaaa tttaaaggta gaattttagt tagactttta gattttaaac acaaaaataa
    10321 ccatacaagc aacaagaagc aaagtgctat attataaatt gtccgtccgt ttacttacat
    10381 aagatcattg gggctatttt taaaagagaa taatagataa taaatgaaaa aaaagtcaag
    10441 tccacaatcc tgtgtaaaat actatacaat gctctcacta ttttttactt ggcaaattta
    10501 atatattttc ttgatgaaga aagccactca tcgtgttgat caataaggac tgctccaatt
    10561 aaacgattgg ctgaaacgca gtttggaaag atacgaataa ttttttctct tctgcgcact
    10621 tcttgattca gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt
    10681 acggtatatt gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat
    10741 tttggttgat cgatataatc atgaatcaat cgatttttag cctcacgagc taagttaata
    10801 tctgtgaact taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga
    10861 atggtggtaa agatatttct taggaagtga acttggcatc tttgccaact tgcgttggtg
    10921 aaggattttc taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttctgta
    10981 ccttgtaaac cgcgttcttt taggtattca aaaaatgtta tccaggtctc ttcgctttcg
    11041 ccactttgaa tcatgaagcc gataatttca cggtcgccat ctttggttat tccaat
//
LOCUS       DACCDF010000087        10767 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCDF010000087 DACCDF010000000
VERSION     DACCDF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.091"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            79..1125
                     /locus_tag="IVK51_002328"
     CDS             79..1125
                     /locus_tag="IVK51_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP6431890.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IVK51_002329"
     CDS             1474..2106
                     /locus_tag="IVK51_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP6431891.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IVK51_002330"
     CDS             2694..4133
                     /locus_tag="IVK51_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6431892.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IVK51_002331"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IVK51_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IVK51_002332"
     CDS             4895..5110
                     /locus_tag="IVK51_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431893.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IVK51_002333"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IVK51_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IVK51_002334"
     CDS             complement(5770..6681)
                     /locus_tag="IVK51_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6431894.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IVK51_002335"
     CDS             complement(6700..7704)
                     /locus_tag="IVK51_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6431895.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IVK51_002336"
     CDS             complement(7701..9824)
                     /locus_tag="IVK51_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP6431896.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..10749)
                     /locus_tag="IVK51_002337"
     CDS             complement(9829..10749)
                     /locus_tag="IVK51_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP6431897.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3293 a         2032 c         2018 g         3424 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccacc
//
LOCUS       DACCDF010000088        10510 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCDF010000088 DACCDF010000000
VERSION     DACCDF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.092"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK51_002338"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IVK51_002338"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP6431898.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IVK51_002339"
     CDS             1085..2005
                     /locus_tag="IVK51_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP6431899.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IVK51_002340"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IVK51_002340"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP6431900.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IVK51_002341"
     CDS             3311..4789
                     /locus_tag="IVK51_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP6431901.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IVK51_002342"
     CDS             5066..7126
                     /locus_tag="IVK51_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431902.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IVK51_002343"
     CDS             7209..7778
                     /locus_tag="IVK51_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6431903.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IVK51_002344"
     CDS             complement(7953..8759)
                     /locus_tag="IVK51_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431904.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IVK51_002345"
     CDS             8897..10411
                     /locus_tag="IVK51_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP6431905.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2071 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACCDF010000089        10433 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCDF010000089 DACCDF010000000
VERSION     DACCDF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.093"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..305
                     /locus_tag="IVK51_002346"
     CDS             87..305
                     /locus_tag="IVK51_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431906.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(594..1106)
                     /locus_tag="IVK51_002347"
     CDS             complement(594..1106)
                     /locus_tag="IVK51_002347"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP6431907.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IVK51_002348"
     CDS             complement(1120..3417)
                     /gene="recJ"
                     /locus_tag="IVK51_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP6431908.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3559..3993)
                     /locus_tag="IVK51_002349"
     CDS             complement(3559..3993)
                     /locus_tag="IVK51_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP6431909.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4003..4791)
                     /locus_tag="IVK51_002350"
     CDS             complement(4003..4791)
                     /locus_tag="IVK51_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6431910.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4788..5729)
                     /locus_tag="IVK51_002351"
     CDS             complement(4788..5729)
                     /locus_tag="IVK51_002351"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP6431911.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5906..6493)
                     /locus_tag="IVK51_002352"
     CDS             complement(5906..6493)
                     /locus_tag="IVK51_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP6431912.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IVK51_002353"
     CDS             complement(6714..8027)
                     /gene="obgE"
                     /locus_tag="IVK51_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP6431913.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8315..9325
                     /gene="gap"
                     /locus_tag="IVK51_002354"
     CDS             8315..9325
                     /gene="gap"
                     /locus_tag="IVK51_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP6431914.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9410..10345)
                     /locus_tag="IVK51_002355"
     CDS             complement(9410..10345)
                     /locus_tag="IVK51_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP6431915.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2888 a         2356 c         1809 g         3380 t
ORIGIN      
        1 atcacttccc tctttacaaa ccgaacaaat attcgtatac taaatatacg aacaattgtt
       61 cgcataaatt aaagaggtga gaattcatga aatcaatgag acgtcttggc ttagtattaa
      121 ctgttttgtt tttaggtatt attattggta atttcggcat acgtccagct ttacttgtgt
      181 ttgttccgtt atttactttg tggttcatgt tgtgggatga aaagaagtac cgtcaatctg
      241 agcggcgcag aattcatgaa gatcatttct atcatgagcc ctactacaga tccaggcaat
      301 cataataagg attgactgct aattattcaa aagacaagaa gttaaacttt tacgggggtg
      361 tgacaaaaat cggtcacatc ctttttttcg aataaacggt gttctaaagc cagtttttcg
      421 gtattaagtc aaattgcgga aaaagtgatt ttttaaactt ctactggtct ttgcttgttt
      481 ccatttataa aaaaatagcg accctcaaaa gtcagataga gaatctgact tttgagggtc
      541 gctttaaaat cgcttcttta agaataacat caagaatagc ttcttttttc tgcttaatat
      601 tccattaatg taatgatgtc atatcctttg attttgtcgc gtccatggag atccatcaat
      661 tcgatcaaaa atgcgcagcc tacgactaca ccgcctaatt gttcgatcag ttcgatcgtc
      721 gctttgattg ttcctccagt agctagcaaa tcgtcacaaa tcagcacacg ttgacctggt
      781 ttaatggcat ccttatgcaa tgtcaatgta tctgttccat attctaatcc ataagtcact
      841 tcgatggttt ctcgaggtaa cttccctttt ttgcggactg gggcaaaacc gactcctaac
      901 tcataagcaa caggacatcc tacgatgaat ccacgggctt ctggcccaac taccatatca
      961 attcgttttt cttttgcata atttacgatt tgtttagtag cttcccgata agcgtcacca
     1021 tctgccatca gtggggaaat atctcgaaac acaattcctt ttgacggata gtcgggaata
     1081 ctggcaatat aatcttttaa atccactctt tgtttcctcc taggttgtta accatttctt
     1141 tatggtagga atatcgctca aaagcaaaaa ttcctcaact ttgattttat tgagccgtcg
     1201 ctggtataat ctgctctcag tcaaaggatg actttctggg ctagcgactt tttgtaaaac
     1261 accgttctct attgtaacaa atttcaattc aaaaaacacc tgtatcataa aaattaataa
     1321 tttttcaggg atacgcaaat attgagctat tgcttttatt ttatgacgta tatcaattcg
     1381 ttcttgagaa tggatcaatt taaacaattt tgcgtactgt tctcttgtgc ctaccccatt
     1441 caaataggcc tcatcaggtg aaatccccat cagataaaca cgattgaatg aataagaccg
     1501 aaaaacttct ttcagatccg ctaaatcatc tggacaatcc ataacaacta gagagtttgg
     1561 ttttcttttt tcaattattg cctttacaga atctagtcct tcgaaaacaa tcgcagcttc
     1621 ctggataatc tcaggcacga atttcaacgt cgaagaatca aatgctaaat aaaggctacc
     1681 ttccgacgtt cggctctgtt cacggaaacg ttttgcccgc caatcaaata cttgcagacc
     1741 acccacagaa aaatcagata ccatcagctg cggtttctta cgcccgttcc attcattgat
     1801 cgaaagcttt cctaccacat ctacctgatt attcagcagt tcttcttctt gagtaccaaa
     1861 accaaaagcg accgcatcaa gctgactcga tgcatcggaa aggacaaatt tcagatgctg
     1921 ttggtttgct ccgattcttt tgatattttc tgcttggact tgtcgaaata aaaactgagg
     1981 caaaggatta tccgtaccaa atggcgccaa tagtttcagc tcatcaattc gctctactgt
     2041 tacttcattt ggcagcaata cttcatcaat tctcaactca ggccctctca tcaagtcaat
     2101 ttgatgatcg actatatatt ggttcatctt ctcctgtagt atagtgacat tttcacttgg
     2161 catcgttaaa ccgactgctg catgatgtcc tccgaagtaa gtaaataaat ctcgcatctg
     2221 gtcaagcatt tcaaaaagat tcaaggcttc aatactacgt ccagatcctt tagctgatcc
     2281 atcttctttt aaagtaagga caagcgttgg tttacccgtt tcattcatta ttttcccagc
     2341 aacaatgccc aaaacgcctt catgccatcc tcggtttacc aatagatgga tatgattctc
     2401 cttattgacc atcgctaatg cttcttgtgt tatctgctct acgattgctt ttctttcttc
     2461 attgatctca ttcaattttt ttgcttgaac ggatgcttcc tcttcatcaa acgtcgcaag
     2521 taacgagacg gcaggacgtg gatcgcccat ccgaccgatt gcatttagtc tgggagcaat
     2581 cgaaaaaccg atcgttgttt cgtctgcgtc cctcattttt acgccgcttt cttcaaatag
     2641 cgcctgaagt cctatccgtt ctgaatgatg aatggcatcg attcctaatg aaacaaggat
     2701 tcgattctca tcagtcaatg aaactaaatc ggcgatcgtt cctatcgcta ctaaatccaa
     2761 aaattctgca ggcggttctt ccaataatgc agaagctact ttgaatgcta ctccgactcc
     2821 tgccaaatct ggaaatggat agtgccccgc tggatgacga gggtgaacaa tcgcataagc
     2881 gtctggtaaa ttctcaggta gttcgtgatg atctgtaatg atcacgtcta caccagcttg
     2941 ctgagcgtat gctaccgcat catttcctgc taccccgtta tcaacagtga tgatcaactg
     3001 gattccttct tcgatctttt cttggtatac cgattggttt ggtccatacc catgctcaaa
     3061 tcgatttggc aggaatactt ctacttctgc accaagtaat tccagtgttt ctttcattac
     3121 cgttgcactg gtgatcccat ctgcgtcata atcaccgtaa acgaggattt tttctccact
     3181 gattacagct tcttggattc ggctgattgc tttatccata tcgtgtatca gatacggatc
     3241 atgtaattct tgttcctttg gatgtaaaaa tcggttgata tccttttcct catggtaacc
     3301 tctttgccac agtaacttac cgacaaatgg agataatttc agttcacgga tcatttggag
     3361 aaaggattca gaaggctctg tctcgcttaa cttccaatca aaatttgctt gtctcacttt
     3421 ctcactccta acttagttat tatagcaaag gaaatcagca aaaaaaagca gaaagaaaga
     3481 ataaaaacag atcagatccg ctagacagat gttcagcagg acctgatctt ttgtacgacc
     3541 atttatgaaa tatccctatt agtcaaaata attgaagctt tgtttgggcg attgagtttt
     3601 ttgttcttcc ggcgctttca attgactgat ttgtgcggtt ttcgcttgta actccgcctg
     3661 ataagcagct tccaactccg cacgttcgtt tctaaactcg cgttgctgtt cttcttttgc
     3721 ttcttcaatt ttcttttgaa gttctgtttg atattctgac atttcctgag tcagctgttt
     3781 gatttgcttt ttttgctgcc atagtgttgc cgaagcagtc aaaaatacaa tcagtgcacc
     3841 aatcagcgca gaaccgatga tcactaagat caatggcgca gaaaaggaag tgaagccaaa
     3901 acttacagga acatttttat tgttcaatac agcaaaaaga acaataatca aaactaaaac
     3961 aaatccaaga atcacattgc tttgcttttt caattccttc acctactttt tgttaaaaat
     4021 tccgctggct aaataatcac caatcgttgg aaacagcgta taaaattttg atgcaacttc
     4081 taaaataaac ggttgattga tttcccgttt tggatgtctc atattgcgga cgatcatttt
     4141 tgccaacttg gcaggttcta aaacgatttg tccgactttt tccaaatagc ttccgcttgg
     4201 atcagcctta tcgaagaaat ttgtctcgat tggacctgga ttcactgtcg taacagctac
     4261 gccaagtggt ttcaattcta atcgcaaagc gttggagaag ccaaggactg caaatttcgt
     4321 ggcagaataa accgttgatt ttgcagttgc catttttcca gccatagagg cgatattaat
     4381 gatatgacct tgtcttcttt ctgccatatc gatcgcaaat ttttgtgtca aaaccatcat
     4441 tcctaagatg ttgacttcaa acatatcgta tgcttttcca aggtcgaatg taaggaagtc
     4501 ttcgaaaatc ccaaaacccg cattgtttac cagaatatcg acgcgcccga cttcttccat
     4561 caccttttca tataattcgt ccacgctttc aggattgctg acgtctacct tgaaagcata
     4621 agcctctttg ccgctcaagc gtctgcactc gtctctgaca ccttctacaa atgccattct
     4681 tcgtgcgcaa cttataacga ttgctccttg ctttgctgct tcataacaga tttgtgcgcc
     4741 cagaccgcct gaactacctg ttactaaaac aactttatct tctaaattca tgcctcttcc
     4801 tcctcgcgaa atggaatctc aatgctatca aaatctttca taatcgtcgt gttttcaaac
     4861 acttcttgtg cttctttttc aagctctaaa gctgcttttc ctaaatatct cgcactgata
     4921 tgagtaagta gcagccgttt tgcctgcgct tcttttgcaa cttcagcagc ttgatgagtc
     4981 gtggaatgga aatagttgtg cgccattctt gcttcatctt tattaaatgt gctttcatgt
     5041 accagaatat cgctattttc cgcaagcaca acggaattat gtgtttttct cgtatcgcct
     5101 aaaatagtca cgattcgtcc ttttttatct ggacctacga aatcttttcc attgatcgtc
     5161 cgcccatctt ctagttggat cgtttcacct tgcttcagtt tgccgtacag tggtccagct
     5221 ggaataccta attcttttag acgatccacc tgcaattctc ctttatgatc atgttcaacc
     5281 acacgataac caaaactgtc gatcccatga tttagttttt ttgtataaac agaaaattgc
     5341 tgatcagtaa aaatcggggc tgtttcattt agctcaatga atttcagcgg ataacttaaa
     5401 cgcgtttgcg aaatacctaa agagactttt atgtattcct ctatcccttt tggcccgtaa
     5461 atctctaatg gcgtatcccc accttgaaat gaccggctgc tgatgagccc gggaagtcca
     5521 aaaatatgat cgccgtgtaa atgtgtaatg aaaattttac cgatttttct cggacgaata
     5581 gtcgttcgca aaatctgcat ttgcgtgcct tcgccacagt cgaacaacca aacttcattg
     5641 cgctcgtcta aaagtttcaa tgctatactt gtcacatttc tatgttttgc aggaacacct
     5701 gcacctgttc ctaaaaattg taattccata atctacctga ctttctttaa gttcctcttt
     5761 aattttaggg taaaaagaag ggataatcaa ccgtaagtcg agaatgggtt caaattaaaa
     5821 ataacgttct aaagcgatag cgttttcttt gaaaattact ataaaaacag cagcgaaccc
     5881 gtttgagatc ctctgctgtt ttttattaat ttgaagatcc agaactttca ttgtcgttgg
     5941 aagtttgcgg atgtttgtcc ttccaagtct tttgtgcagc ttctttcgca tgattttcag
     6001 cttctttcgc atcatgcaca tctttttcga catcttcata atccaagcga gtaatttctc
     6061 cgcctaattc catcattacc aattgattca gtgggcgatt gtcctcttca tcagcaatca
     6121 agatcagtcc tgtagctcct tcaccgattt tatttcctac aaagtcgaac acggtctggg
     6181 catcttttat ttctttggca tcttgtgtcg caccgatcat tcctccggca aaccatccta
     6241 ataaaatccc tagcggacca cctaatacac caaccaacat tccgattaat ccacccgtag
     6301 aagatttgtt attaccggta aagtcgataa aatcttctat tttgaattga tgtgtcccat
     6361 taggatcatg gctgacaact gccatctgtt ctcctttaat cgaacgaaca gcatgtaact
     6421 ttttgatttc tgaaaatgcc tgatatgcct ggctttctac ttgaaaattt aaaatgatta
     6481 ctcgtttgct cattaaaaac gcccctttca taccttcttg ttatttattt tacctttaat
     6541 tgaaaaataa gaaaagaaaa gcactctttt tttccaataa aatagggctg tgacaaaagt
     6601 cgtatcacag ctcctttttc cgaataaacg gcgttcttga agctgatcac ttctgtcaca
     6661 acctccctta aatctgaatc tatatttttt ggtatttgtt tttatttgct tgcctattcg
     6721 acaaattcaa attcaaactc accgatacgt acaaggtcgc catctttcgc accgcgtgcc
     6781 cgaagagctt catccacacc cataccacgt aattgacgag caaaacgcat cactgtttca
     6841 tcatgatcga aattcgtcat ttggaataat ttttcgatct tctcaccaga taagatccac
     6901 gttgcatctg aatcacgatc gatttggaac tcaggacctt ctggattgaa tccatagtgt
     6961 acagtttctt cttccagttc ttcttcgtac aatgggaatt ctggcgttac ttctaacaaa
     7021 tctgctgtag cattcaataa cgcgtccaat ccttgtcgag tcactccaga aattggaaat
     7081 actgggatgt catcagcaaa ttcatcttct ttttccttgt tcagctgttc tttgaatttc
     7141 actagatttt ccggtgcatc cggcatatcc attttgtttg ccacgatgat ttgcggacgc
     7201 tctagcaagc gtaagttata agtagataat tctttgttga tggcaagata gtcttcataa
     7261 ggatctcgtc cttccatccc actcatatcg atcacatgta aaattactcg tgttctttcg
     7321 atatgacgaa ggaattgtgt acccagccca acaccttgcg aagctccttc aatcaagcct
     7381 ggcaagtctg ctgcggcaaa acttctgcca tcacttgtag taaccattcc tagattaggt
     7441 acgagcgtcg tgaaatgata agcgccgatt tttggacgag cagatgaaat aacggatagt
     7501 aaagtcgatt tcccgacaga tggaaaacct acaagtccaa cgtctgccaa tactttcaat
     7561 tctagttcga tttttcgctc ttgtcctggt tcaccgtttt cagcaatttc cggtgcagga
     7621 ttccgaggag aggcaaaacg gatattccct cgtccaccgc gtccaccttt tgccacaact
     7681 agtgtttgac cattttcaat taaatctcct aaaagagcac cagtctccgc atctcggaca
     7741 gtggttcctt gtggtacttt aacataggta tcttctgaac cacgaccgtg catcccttta
     7801 ctcatcccat tttcacctgg ctgcgctttg aaatggcgat tgaaccgaaa atccatcaat
     7861 gtacgtaacc cttcatcgac gatcaagatg acatcgccgc cacgaccacc gtctccgcca
     7921 gccggtccgc cgtcaggaac gtatttttcg cgacgaaatg caaccattcc gtctccgccc
     7981 ttgccagctt tcacatcgat cgttacttga tctaaaaaca tggacataat taagtcctcc
     8041 tattattcat ttatttattt gactgcctga atttccacaa taaacaaaag tcttacttat
     8101 tttatcggtg aaatctgatt ttgtctatca tttcagcaaa aagattgttt accttcttct
     8161 ttttttatca tttggtaagg tttccaacct tttttctgct accaagtata attttttgac
     8221 aaaatattct ttgcgtagca gaataacagt gtattaattg tattaattga ggatgccgtt
     8281 tcacagcacc cctaataaag gaggaacttt tgatatgaca gtaaaagtag gtattaacgg
     8341 atttgggcgt attggccgtc tggctttccg ccgcatcaaa gaagtctcag atgatattga
     8401 agtagtagca atcaacgact taacaagtcc aactatgtta gcacaattat tgcaatttga
     8461 ctctactcat ggtacttatc ctggcacagt tactgcaaca gaagactcaa tcgtagtgga
     8521 tggcgaagca actcgtgttt atgcagaacc cgacgcaagc aaaatccctt gggcaaaaga
     8581 aaacggtgta gatatcgtac tagaatgtac tggtttttat acatctgaag aaaaagcaaa
     8641 agcgcattta gatgctggtg tcaaacgagt agttatttct gccccagctg gtgctatgaa
     8701 aaccatcgtc tataacgtca atgatgatac attagatgaa aacgacaaaa tcatctcagc
     8761 aggttcatgt acaacaaact gtttagcacc tatggcttat ttcttaaata atgaatttgg
     8821 gatcgaagtt ggtacaatga caacagttca tgcctacact tccacacaaa tgctcttaga
     8881 tggacctgta aaaggcggta atttgcgtgc tgctcgttca gctgctgaca atactatccc
     8941 acattctact ggtgctgcga aagcaatcgg cttagttatc cctgagctac aaggaaaatt
     9001 acaaggacac gcacaacgtg ttccagtcgt tgatggttca ttaactgaat tggtttcagt
     9061 attgaaaaca aaagtaactg ctgatcaagt aaacgaagca atcaaaaaac acacagttga
     9121 caatccttca ttcgggtatg atgaccgtca aatcgtttct agcgatgtca ttggaacaac
     9181 tcaaggttcg atctttgacc caacgcaaac agaagtaaca actgctggtg atttccaact
     9241 tgttaaaact gttgcatggt atgacaatga gtatggattt acttgccaaa tgattcgctt
     9301 actagaaaaa ttcgctaact tgtaatttga tagatcaatc gataaaagaa gcctttgact
     9361 ggatccagtc aaaggcttct ttcttttcat cccctacttt taaactcgtt caatgaacat
     9421 attggcttaa aaattgttgg actacttgct gatatttttc aggatctgtc ttgtatgctt
     9481 ccgcatgctc ggctcctttt acaacatact tttcttttgg tcccttcgtt gcacgataga
     9541 ccttatctaa catttcaaat ggtacaaatg tatcttcgtc tccatgtata aagagcattg
     9601 ggcgctggtt tttcttcaat tgttccactg catttgcttc accgaaaaag tagccagcac
     9661 ggacttttgt cactagactc gttacttgca taagcggaaa tgctggaaga ctaaacatat
     9721 cgtctagctg ataagcaagc tcatcatgaa cggaagaata accgcaatct tcaatgattg
     9781 ctttcacatt atctggtagt ttttctccgc tagtcatcat aactgttgct gctcccatgc
     9841 tgacaccgta tagaacgatt tcttgcgatt ttccattgtt ttctagcact ttgtttatcc
     9901 actgtacata gtctttacgc tctggccatc caaatccaat gtaatcccct tcgctttttc
     9961 catgtcctct tgcatcagga acaaggacat tgtaacccat atcatgaaac atctttgcgt
    10021 aattggtcat cgtttcagcg tttcccatat agccatgagc cacaagagct gttttttcac
    10081 ttttcgtttc agcaggaaga taaatcgctg ataatttcag accgtctttt gaagtgatgg
    10141 tccaatcgct tcggttttct tttgaactaa accatttatg tgcatccgtt tctttggatg
    10201 tctgtgttgt accaggtgta tcttcgtcta aaaagtcttt ctcagaaggc acaacagcat
    10261 aattataaaa ataattccct gcaaacacta acgcaacaat cagagctaaa gcgacgccta
    10321 aaaggatttt catccaaagt ttcataaatc atccatctca tttctctttt ttcatataac
    10381 cttgaattag tataatcaaa gaatctctac tatgcaatgt tcatttttat aac
//
LOCUS       DACCDF010000090        10218 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCDF010000090 DACCDF010000000
VERSION     DACCDF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.094"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..410)
                     /locus_tag="IVK51_002356"
     CDS             complement(<1..410)
                     /locus_tag="IVK51_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6431916.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHFVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEK"
     gene            complement(492..842)
                     /locus_tag="IVK51_002357"
     CDS             complement(492..842)
                     /locus_tag="IVK51_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431917.1"
                     /translation="MINNYLHYKAINWNVIEDELDNVVWERATSLFWLDTRVPIENDR
                     SKWANLQLQEQEQLNRLLILLTNIATYQSNELGEIIRDSARSQQEIAIINNFQFTEMV
                     HTKAYNRILSIRQF"
     gene            complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK51_002358"
     CDS             complement(891..1334)
                     /gene="nrdI"
                     /locus_tag="IVK51_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6431918.1"
                     /translation="MQIIKVYYISLSGNTTSFLADLDTYCQKQYQKKVDYVNVKDLVK
                     LGKPLTFDINESYFVFLPTYLEGGNGIETGDVEILTTPLRQVITYNKNRDYCLGIIGS
                     GNRNFNKQFCLTAYQYADDFGFTVLDEFELKGTTEDIERIAETMQ"
     gene            1481..2146
                     /locus_tag="IVK51_002359"
     CDS             1481..2146
                     /locus_tag="IVK51_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016896696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP6431919.1"
                     /translation="MKNSGCLCTVPLFASLSLDEKNEIKCLLHHQTFKKGEIIFSPSS
                     EEQLLIISLGAMKIYRISRTGKEQLIRIVKEGDYDGENYLFGLPNDCLYGEAVEETKT
                     CTLYKSDFNKVMARYPQLSYRLLQLNACKTVAVEKQLQLLAFDRIEDRLALYLNQLSI
                     AAKMGEPITLPISMRELANYLATSPETISRKLRDFQERQLITRKGKQITLLEEFWFIF
                     NYI"
     gene            2136..2591
                     /locus_tag="IVK51_002360"
     CDS             2136..2591
                     /locus_tag="IVK51_002360"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAP6431920.1"
                     /translation="MIYKEEDIKIGIVIARFNDFLTKSLLSGCEDGLTRSGVLTENID
                     VAWVPGAYEIPLTAATMAETGKYDAIITLGAVIRGATSHFDVVVNQASSGVSQVGLTH
                     KLPVIFGILTVENIEQAIERSGTKAGNLGFTYATNTIEMINLFKEIKQQ"
     gene            2741..4120
                     /locus_tag="IVK51_002361"
     CDS             2741..4120
                     /locus_tag="IVK51_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAP6431921.1"
                     /translation="MTIQFKQTLAPTKDNSGKPIILSDATFAQRNEKVLQLMREYDFS
                     SLMIYADKEHGSNFEYLTGFIPRFEEAIQVINQDGTSTLILGNENYNKTSMARTQSEG
                     ILCPFFSLPNQPMDFTKTFADYLKDVKVDASKRIGLVDWKLLSNDMDNFHNHHSIPAF
                     IVDGIHEVFGSDKVVNATQLYMHPKEGARVTNNAEEIARYEYGASLASDAVLEAMNQL
                     TVGQSELAAGNHLNRDGQYPSVVTIAAFGDRFEGANIYPTDNTLNDGDKVALTVAYKG
                     GLSSRSGYAVKEESQLAEHDPGYLEEVVYPYFQAYKWWLDNLTIGRKGGDFYQAFSDF
                     YPQATYGWNLCPGHLVADEEWLSSPFYQDSDATIQSGYIFQLDFIPSQAGHNGVSAES
                     TIAVADAALREQIKAEQPELWARICNRKDYLKEALGLELSEHLLPLASTFAYYRPFLL
                     DKKTAIYFG"
     gene            4257..5099
                     /locus_tag="IVK51_002362"
     CDS             4257..5099
                     /locus_tag="IVK51_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP6431922.1"
                     /translation="MPLDEQIELLFTIDEGYLNPLKVALTSIRQNNPGQAFRIWLIHE
                     SIAMHTIRELQKLTDYLQFGFEAIKIDGSRWNSAKTEDRYPKEMYFRLLAGEILPKEM
                     KRVIYLDPDILVVNPLLDLWQTDLEGYMLAAATHVGLTDVSTRVNQMRLDVDHAYYNS
                     GVMVIDLDKAREIIKWSDIAQMIEKYNLLLILPDQDILNRLYGKYTKEIPEEIWNYDT
                     RKYMRYFTKSLAQHDIHWVMAHTAILHFCGGPKPWDDKHDNRFTSLYLTYQNQLKRIE
                     DLMK"
     gene            5173..6621
                     /locus_tag="IVK51_002363"
                     /pseudo
     CDS             5173..6621
                     /locus_tag="IVK51_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            6736..8508
                     /locus_tag="IVK51_002364"
     CDS             6736..8508
                     /locus_tag="IVK51_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP6431923.1"
                     /translation="MNNNETKKTYELRITADEIKSTDPTFTMKNLSLGDNYQPKDAYF
                     RTAEGEITRRNYGKNERKISNQTLPRLAIQMFEQSIVSLPESERATFPVCRYHSGDEI
                     IRGIFPDVDSFKKKRKSIEFLIYKYNEDKQIVIYCWSIFSTLLFVQECLKRFGTSGDE
                     FILVYKGKEKKEIRVGETQTMTNHLLKSYVLHLLKSKNLILRGAPGTGKTFLANEMAA
                     DVVSNGRTTQINELSEDEKSRIGFVQFHPSYDYTDFVEGLRPTTSDDGVVSFTLKDGS
                     FKSFVEKAKKTKKVNGQDNFEEAWAKFFDAVTEASIEGEGYNKLKTLTGKPVRNLLAY
                     ERNEIQGVYPAKTNTYLNHDQIYNVYRGLPGTPKGGFDAYRKAIVKHLKEEFGLKNYT
                     ASTVDSTNNQNYVFIIDEINRGEISKIFGELFFSIDPGYRGHKEGVYTQYANLHENPE
                     EKFYIPNNVYIIGTMNDIDRSVDTFDFAMRRRFTFLEIKAEDSAQNMLTQEKTKELMT
                     RLNEAIVAKEKGGLSTDYQIGASYFLSIDNGEETMEQLWDTKLYPLLKDYFHGEHKAK
                     EKLEILEKVYSNDGDLDEAETSTR"
     gene            8483..9775
                     /locus_tag="IVK51_002365"
     CDS             8483..9775
                     /locus_tag="IVK51_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine 5'-monophosphate oxidoreductase"
                     /protein_id="HAP6431924.1"
                     /translation="MKLKPLHDNQEINDSVVQHPIITNLVNRDLSSLEKENFIIFPQQ
                     LNQSKDLSEDNLIFQSRNGKIWTCNVVGILSDSTDELRIHSRFANQMKHEDFFLRYML
                     QRVLNYNVINDHFSASKKMTYYDLLVFLFPYYLNEAMRKGTYKEYVKREYNNANIKGA
                     VDVAKHIRSNVPFVGKVAYRTREFSYNNHLTQLIRHTIEKIQNEYDFLLSGDEDTKEN
                     VVLIKQTTPGYARLNQFNILQENILHPVKHSYYEEYSALQQICIQILSEEKSGFGSDK
                     NQIHGIIIDVSWLWEEYIGKVTGWKHYGRDKGLATMHLFQEPNRSPRYPDFTFNNIPI
                     DTKYKKHLDTRNDYNQLVTYIHIMNLDQADTMKGGFLQPTSDPLPANHGYKKIGILSG
                     LGGEIFTYHFFIPQAASSYLEFVEQMREVEAALQTTFQ"
BASE COUNT         3534 a         1665 c         1935 g         3084 t
ORIGIN      
        1 cttttttctt tatgtccgtt tggttttctt ttccctttca ttggtaaatc gtgagggtca
       61 aaggttttat ctttaaacat ccgatatagt gtacgtaccg aacaagcaat aggaaactct
      121 gctcgaccaa taaggacatc tggagtccac ccttgagcca cctttttttg aatgtattct
      181 tgttgcccat ctggtaacac aatcggacgt cttccgcagt tacgtttgtt tttcttatat
      241 tgttcatagt agcttattgc gcttccacca gatttaagaa aacgatagac attataaatg
      301 gtttgtcgag aacgttgtac tagatgggct acttttgaaa caaagtgtga ttgattgaaa
      361 taagcttcta tcatcacgag ttcagttggt gtaagatggg tataggtcat ttgtactcac
      421 tccttataat tctttcgacg gaactatttt gagtgtagca caaatgactt ttttagttgt
      481 ctagcttaat tttagaattg gcgtatgctt aaaatgcggt tataggcttt tgtatgaacc
      541 atttcagtaa attgaaaatt atttatgatt gcaatctctt gttgggaacg tgcactatct
      601 cgaataattt ctcctaattc attggattga taggtagcga tattagtcaa aagaatcaac
      661 aagcgattga gctgctcttg ttcttgtaat tgtaaatttg cccattttga tcgatcattt
      721 tcgatgggga cacgtgtatc caaccaaaaa agtgaggttg ctctttccca tacaacgttg
      781 tctaattcat cctcaatgac attccagtta attgctttat agtgtaaata attatttatc
      841 atagtacctc ctaaaatatt gtttgaaagg ggatgatatg gtgtcatttt ttattgcatt
      901 gtttctgcaa ttctctctat atcttctgtc gtacctttta gttcaaattc atccaacacg
      961 gtaaaaccaa aatcatcggc gtattgatag gctgttaggc aaaattgttt attgaagttt
     1021 ctgttgccac taccaataat tcctaaacaa tagtctcggt ttttattgta agtaatcact
     1081 tggcgcaatg gtgttgtcaa aatttcgaca tctcctgttt caataccatt tccaccttca
     1141 agataagtag gcagaaatac aaaataggat tcattgatgt caaaagtgag tggttttcct
     1201 aatttgacta aatcttttac attcacataa tctaccttct tttgatattg tttttgacaa
     1261 tacgtatcta aatctgcgag aaaactcgtg gtatttccac ttaaactaat ataatagact
     1321 ttaattattt gcatgtatca cacctctttt taataaataa ccacatatat agtgcttgtt
     1381 ttaattagaa acgctatata tagtgtttat tgtacatgac atatcgttat agaaaattga
     1441 cgatggtcaa ttattttgat tttagaggag agaaaattac atgaaaaatt caggttgttt
     1501 atgcaccgtt ccactcttcg cctctctttc attagatgaa aaaaatgaga taaagtgttt
     1561 gcttcaccat cagacgttta aaaaaggaga aattattttt tcaccaagta gtgaagaaca
     1621 actgttgatt atatctttag gggctatgaa aatatatcga attagtagaa ctgggaagga
     1681 gcaattaatt cgtatcgtaa aagaagggga ttacgacggt gagaactatc tctttggtct
     1741 acccaatgac tgcctatatg gtgaagctgt tgaagaaacc aaaacttgca ctctctataa
     1801 atccgacttt aataaagtga tggcacgcta tccacagctc agttatcgat tgttacaatt
     1861 aaatgcttgc aaaacagtag ctgtggaaaa acagttgcag cttctagcgt ttgatcgtat
     1921 agaggaccgg ttagccttat atcttaacca actatctata gcagcaaaaa tgggggagcc
     1981 tattacttta cctatctcta tgagagagtt ggcaaactat ctagctacca gtcctgaaac
     2041 tatttctcgt aaattaagag attttcaaga aagacaattg attactagaa aaggaaagca
     2101 gattacacta ttagaagaat tttggtttat atttaattat atataaagaa gaagatatta
     2161 aaattgggat tgtcattgca cgattcaatg attttttaac taaaagctta ctaagtggtt
     2221 gtgaagatgg ccttacgcgt tcaggcgttt tgacagaaaa tatcgatgtt gcttgggtgc
     2281 cgggggccta tgaaatccca cttacagccg caacgatggc tgaaacaggt aaatatgatg
     2341 ccattattac cctaggtgcg gtaatcagag gagctacaag tcactttgat gtggtggtta
     2401 accaagcgtc aagtggggta tctcaagtag gcttaaccca taaattaccg gtcatctttg
     2461 gtatcttaac cgttgaaaac atcgaacaag ccattgaacg ctctggtact aaagctggta
     2521 accttgggtt tacttatgca acgaatacta ttgaaatgat taatttattc aaagaaatta
     2581 aacaacaata agtcatcatt ctatatgaaa atatctgtaa cagcataggg ctagctttaa
     2641 ttttcaatca tggtaagatg gaagatagga aaataaagcg cattcaatta attatcaagc
     2701 ctttctgatg atttgcgcaa aaagcttaag gagacttcat atgacgattc aatttaaaca
     2761 aacattagcg ccaaccaagg ataattctgg aaaaccaatc attttaagtg acgctacctt
     2821 cgctcaacgt aatgagaaag tccttcagtt aatgcgtgaa tatgacttct catcattgat
     2881 gatttatgca gataaagaac acggtagtaa ctttgaatat ttaactggtt ttattccacg
     2941 gtttgaagaa gctatccaag taatcaacca agacggaacg tcgacactga ttttaggtaa
     3001 tgagaattac aataaaacca gtatggcacg tacacaaagt gaaggtattc tttgcccatt
     3061 cttctcttta ccaaaccaac ctatggattt cactaaaact tttgctgact atcttaaaga
     3121 cgttaaagta gatgcttcaa aacgaattgg tttagtagac tggaagcttt tatctaatga
     3181 tatggacaac ttccacaacc atcattcaat accagcattt attgtagatg gtatacatga
     3241 agttttcggt tctgacaagg tagtgaatgc aacccaacta tacatgcatc caaaagaagg
     3301 tgctcgtgtg acaaacaacg cagaggaaat cgctcgttat gaatatggtg cttcccttgc
     3361 ttctgatgct gttcttgaag caatgaacca gctaaccgtt ggccaaagtg agttagcagc
     3421 aggtaatcac ttaaatcgcg atggtcaata tccatctgtt gtgaccattg cagcctttgg
     3481 tgatcgcttt gagggtgcta atatttatcc aactgataat acgctaaatg atggcgataa
     3541 agttgcatta actgtagcct ataaaggagg cttgtctagc cgctctggct atgcagtaaa
     3601 agaggaaagt cagctcgctg aacatgatcc aggatactta gaagaagtgg tttacccata
     3661 tttccaagcc tataaatggt ggctagataa tttaaccatt gggagaaagg gtggcgattt
     3721 ctaccaagca ttcagtgact tctatccgca agctacttat ggctggaatt tgtgcccagg
     3781 gcatttagta gctgacgaag aatggttaag ctcaccattt tatcaagact ctgatgcaac
     3841 aattcaaagt ggctacatct tccaactaga ctttattcct tcacaagcag gccacaatgg
     3901 tgtttcagct gaatcaacga ttgcggttgc ggacgcggca ctacgtgaac agattaaggc
     3961 tgaacaacct gaattatggg cacgtatctg taaccgaaag gattatctaa aagaagcatt
     4021 aggtttagaa ttatcagaac atttactacc cttagcgagt acctttgcct actatagacc
     4081 tttcttatta gacaaaaaaa ctgctatcta ctttggataa aaaaatgccc ctcaagattg
     4141 tttgccaaac ttgagggttt tttgtctatt tttaagtgaa taatgacgga ctatccaaac
     4201 ggaatcgcca taaagatgtt aatatatgta taatattata aagaaattgg aggacaatgc
     4261 ccttggacga gcagattgaa ttattattta ccatagatga aggctatttg aatcccttaa
     4321 aagtggcttt aacatcgatt cgtcagaata atccaggcca agctttccgc atttggttga
     4381 ttcacgaatc cattgcaatg catacgattc gcgaattgca gaagctaacg gattatctac
     4441 agtttggttt tgaagcgatt aaaattgatg gtagtcgatg gaatagcgct aaaactgaag
     4501 atagataccc aaaagagatg tatttccgcc tgcttgcggg tgaaatatta cctaaagaaa
     4561 tgaaacgcgt gatctattta gacccagata ttttagtggt taacccttta ttagatttgt
     4621 ggcaaacaga tttagaaggt tatatgctag cagctgctac acatgttgga ttgacggacg
     4681 tttctacacg agtaaaccaa atgcgacttg acgttgacca tgcttattac aattctggtg
     4741 tgatggtgat tgatttagat aaagctagag aaattattaa atggtcagat attgcacaaa
     4801 tgattgaaaa atataacttg ctattaatcc tacccgacca ggatatcctc aaccgattat
     4861 atggtaaata cactaaggaa attccagaag aaatttggaa ttatgatacg cgtaaatata
     4921 tgcggtattt caccaaaagt ctcgctcaac atgatattca ctgggtgatg gcgcatactg
     4981 ctattctcca tttctgtggt ggcccaaaac catgggacga taaacatgat aatcgtttta
     5041 ccagtcttta tcttacctat caaaatcaat taaagcgtat tgaagatttg atgaagtaaa
     5101 gtgaaacaag tgtttttcat tacctacggt ggtttaatgc ttatttatta tcgttaaatc
     5161 atgcttatac actttacaaa atttgatttt cgaaatggcc gtccaacgaa gtctgcgatc
     5221 ttaaccacga gttccattga catggccaaa aagtattatc gagcgatcaa agaaatgacg
     5281 aaagaacctg actggttaac aaaagagttt cctaatcagc ccattcgtga aggaagaacg
     5341 atggaggatc ctgattttcc acgaatcgct attacctatt cattagaaga aaataaccaa
     5401 gattcaagtg cccaacaaga agaaatgaaa aaaattattg aggaatataa tcagtatttt
     5461 gatactgcct ggtcattagc agacattgag cgttataacg gagacatcaa taattgtttg
     5521 gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa tcgatttagt aatcgtagtg
     5581 gatcgtctat tgacaggttt tgacgcacct acgattcaaa ccctatttgt ggatcgtaat
     5641 ctagagtatg caggattgat tcaagctttt tcacgtacga accgtacgta tcctgaaaag
     5701 acaaaaggat tgattgtgac gtttcgtaag ccagcgacaa tggagaagaa tgtcgaggat
     5761 gcgacaaaac tgtattcaga agcaaaagaa gaatcgggtt tgatttatcc aagttatcag
     5821 gaatccaaaa aaagattcaa gcaagcgtat ggtaagttaa aggaggtttc atccatgaaa
     5881 tccattgatg aacatacgcc acttgaaatc cgtatcgagt acgttaaagc atttcaagaa
     5941 ttgaatcgtg cgtatgaatc gttagtgact tatgacgatt acaatgatga catggtgcag
     6001 tcttcaactt tgaacaatca aattcaactt ttggaagaac aaattggcgt gtacgagacc
     6061 gtgaaggggt cattaataga ggaagaacca gaaaaagagg gagaagattt ttcaacgatc
     6121 gaattttata gtgaaaatac cactaagttg tatgatattg attctactta tattgatcaa
     6181 ttactaggaa cttatgctgc gaatagttca gatgcacgag atgaaattga aaaagctttg
     6241 gctaaattga ataaaacaga aggagtcaaa gaagtttacc gacaaatttt aaatgcattt
     6301 gatgtgggga ctttagatag caatgaagat atttttgtca ttaaacgtcg ctattttaca
     6361 caagcaagtg atgaattgat tcatgccttt tccaatgagt ggtttgtttc tgagaaagaa
     6421 ttacatgctt ctaatctgca atatatgcca ggtgaagatc caattcctaa tatgaaagca
     6481 attatcaaca gtaaggatta cgaaggatat aaagccaaac atccaaaagc gaagccattt
     6541 aaatacccac aagaaatgaa gcgagcctgg cgtaaaatgt tagatgatga gttgattcca
     6601 cttgagaatg aattgaggta aacgagaaaa ttgaagaaat tgtatgataa acatgttaat
     6661 aaatgatgct cgaaataaaa tttggtttta tttttacatt gatacggtaa ataaaatttg
     6721 aaagtagaag aaaatatgaa caacaatgaa acaaaaaaaa catatgaact aagaataact
     6781 gctgatgaga ttaaatcaac agacccaaca tttactatga agaatctaag cttaggggat
     6841 aattatcaac caaaagatgc ttacttccgt acggcagaag gcgaaataac tagaagaaac
     6901 tacggtaaaa atgaacggaa aatttcaaat caaacattac cgcgattagc tattcaaatg
     6961 tttgaacaat cgattgtttc tctaccagaa agcgaaagag caacatttcc tgtgtgtcga
     7021 tatcactcag gagatgagat aatacgaggg atttttccag atgttgacag tttcaaaaaa
     7081 aaaaggaagt ccattgaatt tttgatttat aaatataatg aagacaagca gatagtcatc
     7141 tattgttgga gtattttttc aactttatta tttgtacaag aatgtttgaa acgttttgga
     7201 acatctgggg atgaatttat tcttgtttat aaagggaaag agaaaaagga aatacgagta
     7261 ggagagactc agactatgac caatcatttg ctgaaatcat atgttttaca tttactcaaa
     7321 tctaaaaacc ttattctacg tggcgctcct ggaacaggga aaacattcct tgcaaatgaa
     7381 atggcagctg atgtcgtttc aaatggcaga actacacaaa tcaatgaatt gagcgaagat
     7441 gaaaagagcc gaattggatt tgtccagttt catcctagct atgattatac tgattttgtc
     7501 gaaggtttac gtccaactac atccgatgat ggcgttgtta gtttcacctt gaaagatgga
     7561 agctttaaga gctttgttga aaaagcaaag aaaacaaaga aagtaaatgg acaagataat
     7621 tttgaagaag cttgggctaa atttttcgat gcggtaacgg aagctagtat agagggagaa
     7681 ggctacaata aacttaaaac attgacaggt aaaccagtac gcaatcttct agcctatgaa
     7741 agaaatgaaa ttcaaggtgt ttatcctgct aaaaccaata cttatctaaa ccatgaccaa
     7801 atttataatg tctatcgtgg attgccaggt acgccaaaag gtggatttga tgcttatcga
     7861 aaagcgattg taaaacattt aaaagaagaa tttggcttaa aaaactacac ggcgtcaacg
     7921 gtagattcta cgaataacca aaactatgtg tttatcattg atgaaattaa tcggggcgaa
     7981 atttccaaaa tttttggcga attatttttc tcaattgatc caggatatcg tgggcataaa
     8041 gaaggtgttt acactcagta tgcaaacctc catgaaaatc cagaagaaaa attctatatt
     8101 cctaataatg tttatattat tgggacgatg aacgacattg accgttctgt tgacactttt
     8161 gattttgcta tgcgccggcg atttactttc ttagaaataa aagcagaaga ttcagcacaa
     8221 aatatgctga ctcaagagaa aacaaaagaa cttatgacgc gtttaaatga ggcgatcgtt
     8281 gccaaagaaa aaggtggatt gtcaactgat taccaaattg gtgctagtta ctttttaagt
     8341 atcgataatg gtgaggagac aatggaacaa ttatgggata ctaagttata tccattattg
     8401 aaagactatt ttcatggtga acataaggcg aaagaaaaat tagaaatact agaaaaggta
     8461 tactctaatg acggtgattt agatgaagct gaaacctcta cacgataatc aagaaatcaa
     8521 tgactcagtt gtgcaacatc caatcattac gaacttagtt aatcgtgact taagtagttt
     8581 agagaaagaa aattttatta tctttccgca acagttaaat caatccaagg atctaagtga
     8641 agataacttg atatttcaat caagaaatgg aaaaatttgg acttgtaatg tcgtaggtat
     8701 ccttagcgat tcaacagatg aattaagaat tcattctaga ttcgctaatc aaatgaagca
     8761 tgaagatttc tttttacgtt atatgcttca gcgtgttttg aattacaacg tgattaatga
     8821 tcattttagt gcatctaaaa agatgacata ctacgatttg ttggtatttc tctttcctta
     8881 ttatctgaat gaagcaatgc gtaaagggac atacaaagaa tatgtcaaac gagaatacaa
     8941 taatgccaat atcaagggag ctgttgatgt tgcaaaacac atccgttcga atgttccttt
     9001 cgttggaaaa gttgcttatc gaacacgaga gtttagctat aataatcacc taacccagct
     9061 tattcgtcat actattgaaa aaatccaaaa tgagtacgat ttcttactta gtggagatga
     9121 ggatacgaaa gaaaacgtgg ttctaatcaa acaaacaaca cctggttatg ctagattaaa
     9181 tcaatttaat atccttcaag agaatatcct tcatccagtc aaacatagtt attacgagga
     9241 gtattcggct cttcaacaaa tctgtatcca aattttatcg gaagaaaaat caggatttgg
     9301 aagtgataaa aatcaaatac atggaatcat tatcgatgtg tcttggctat gggaagaata
     9361 cattggaaaa gtcactggtt ggaaacatta tggcagagat aaagggttag caacaatgca
     9421 tttatttcaa gagcccaacc ggtccccacg atatccagat tttacattta ataatatacc
     9481 aattgataca aaatacaaaa aacatcttga tactagaaat gactataatc agttagtcac
     9541 atatattcat ataatgaatt tagaccaagc agatacaatg aaaggcggct ttctgcaacc
     9601 tacttccgat ccccttcctg caaatcatgg gtataaaaaa ataggcatat taagcgggct
     9661 aggaggagaa atattcacat atcatttttt cattccgcaa gctgcgtcaa gttatttgga
     9721 atttgtagaa cagatgagag aggtagaagc agcactacaa actacttttc aataagtagg
     9781 aattatagaa attttactca aaaaatcttc attaaaaaat ctgactaaat aagttgagaa
     9841 aacagaatat acttcaatat ccagtcattt ataaatatca ttcactcatg atacccagct
     9901 ccgtatcaca aataatgata ttttttaaga gaaaaaatta tccctatttt aaataatagc
     9961 aataggtagt ggtagagaga aatttataaa taactttgtt atttgctttg gtagccaagt
    10021 aagttgcaaa agaaaaatag atactgttaa aaagataatg tagacataga aacaaatacg
    10081 taaaatttta gttagaatga tggcaaggag aaaaaacatg atagatgaaa aaaagtgttt
    10141 aaaaaatcta ttggaagata tggatgatat tgaagaaaaa ctatctaaat ggactgggaa
    10201 aacaaatata tttcagaa
//
LOCUS       DACCDF010000091         9873 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCDF010000091 DACCDF010000000
VERSION     DACCDF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.095"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(22..267)
                     /locus_tag="IVK51_002366"
     CDS             complement(22..267)
                     /locus_tag="IVK51_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431925.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(381..959)
                     /locus_tag="IVK51_002367"
     CDS             complement(381..959)
                     /locus_tag="IVK51_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP6431926.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1036..1566)
                     /locus_tag="IVK51_002368"
     CDS             complement(1036..1566)
                     /locus_tag="IVK51_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP6431927.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IVK51_002369"
     CDS             complement(1570..2430)
                     /gene="rlmB"
                     /locus_tag="IVK51_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP6431928.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2423..2818)
                     /locus_tag="IVK51_002370"
     CDS             complement(2423..2818)
                     /locus_tag="IVK51_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6431929.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2815..4227)
                     /locus_tag="IVK51_002371"
     CDS             complement(2815..4227)
                     /locus_tag="IVK51_002371"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP6431930.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IVK51_002372"
     CDS             complement(4224..4760)
                     /gene="cysE"
                     /locus_tag="IVK51_002372"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP6431931.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5082..6542)
                     /locus_tag="IVK51_002373"
     CDS             complement(5082..6542)
                     /locus_tag="IVK51_002373"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP6431932.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6712..7869)
                     /locus_tag="IVK51_002374"
     CDS             complement(6712..7869)
                     /locus_tag="IVK51_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP6431933.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IVK51_002375"
     CDS             complement(7903..9273)
                     /gene="radA"
                     /locus_tag="IVK51_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP6431934.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9336..9863)
                     /locus_tag="IVK51_002376"
     CDS             complement(9336..9863)
                     /locus_tag="IVK51_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP6431935.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2508 a         2214 c         1784 g         3367 t
ORIGIN      
        1 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
       61 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
      121 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
      181 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
      241 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
      301 tttttaaaat tttcaagttt accataaaaa aaaagaaatg caataaaaat aaataagaag
      361 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
      421 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
      481 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
      541 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat cgctaaaggt
      601 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
      661 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
      721 atttgattgc ttataatgct tttataataa cttccaaagc taacaccttt gcttttttcg
      781 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
      841 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
      901 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
      961 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
     1021 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
     1081 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
     1141 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
     1201 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
     1261 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
     1321 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
     1381 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
     1441 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
     1501 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
     1561 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
     1621 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
     1681 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
     1741 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
     1801 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
     1861 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
     1921 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
     1981 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
     2041 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
     2101 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
     2161 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
     2221 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
     2281 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
     2341 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
     2401 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
     2461 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
     2521 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
     2581 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
     2641 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
     2701 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
     2761 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
     2821 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
     2881 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
     2941 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
     3001 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
     3061 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
     3121 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
     3181 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
     3241 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
     3301 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
     3361 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
     3421 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
     3481 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
     3541 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
     3601 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
     3661 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
     3721 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
     3781 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
     3841 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
     3901 tgtagcaggc tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
     3961 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
     4021 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
     4081 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
     4141 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
     4201 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
     4261 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
     4321 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
     4381 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
     4441 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
     4501 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
     4561 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
     4621 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
     4681 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
     4741 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
     4801 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
     4861 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
     4921 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
     4981 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
     5041 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
     5101 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
     5161 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
     5221 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
     5281 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
     5341 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
     5401 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
     5461 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
     5521 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
     5581 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
     5641 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
     5701 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
     5761 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
     5821 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
     5881 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
     5941 gcaacagcaa aattataagt tggcatacca tcgcgttttt gtatgacaaa gtcgccaccg
     6001 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
     6061 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
     6121 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
     6181 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
     6241 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
     6301 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
     6361 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
     6421 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
     6481 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
     6541 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
     6601 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
     6661 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
     6721 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
     6781 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
     6841 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
     6901 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
     6961 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
     7021 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
     7081 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
     7141 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
     7201 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
     7261 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
     7321 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
     7381 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
     7441 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
     7501 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
     7561 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
     7621 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
     7681 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
     7741 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
     7801 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
     7861 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
     7921 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
     7981 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
     8041 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
     8101 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
     8161 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
     8221 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
     8281 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
     8341 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
     8401 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
     8461 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
     8521 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
     8581 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
     8641 aatggctatc ccatttgttt tagcgatttt gagtagttct gcagtggttt ctcttacttg
     8701 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
     8761 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
     8821 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
     8881 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
     8941 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
     9001 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
     9061 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
     9121 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
     9181 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
     9241 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
     9301 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
     9361 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
     9421 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
     9481 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
     9541 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
     9601 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
     9661 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
     9721 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
     9781 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
     9841 acttcaaatc ctctttttct caccggtttt cct
//
LOCUS       DACCDF010000092         9699 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCDF010000092 DACCDF010000000
VERSION     DACCDF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.096"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1023
                     /locus_tag="IVK51_002377"
     CDS             <1..1023
                     /locus_tag="IVK51_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP6431936.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1034..3043
                     /gene="topB"
                     /locus_tag="IVK51_002378"
     CDS             1034..3043
                     /gene="topB"
                     /locus_tag="IVK51_002378"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP6431937.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            3389..3985
                     /locus_tag="IVK51_002379"
     CDS             3389..3985
                     /locus_tag="IVK51_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6431938.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPEKTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3998..4897
                     /locus_tag="IVK51_002380"
     CDS             3998..4897
                     /locus_tag="IVK51_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP6431939.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            4900..5031
                     /gene="ssb"
                     /locus_tag="IVK51_002381"
     CDS             4900..5031
                     /gene="ssb"
                     /locus_tag="IVK51_002381"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6431940.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            5053..5385
                     /gene="ssb"
                     /locus_tag="IVK51_002382"
     CDS             5053..5385
                     /gene="ssb"
                     /locus_tag="IVK51_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6431941.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            5430..5663
                     /locus_tag="IVK51_002383"
     CDS             5430..5663
                     /locus_tag="IVK51_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431942.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(5822..5944)
                     /locus_tag="IVK51_002384"
     CDS             complement(5822..5944)
                     /locus_tag="IVK51_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP6431943.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            6134..6358
                     /locus_tag="IVK51_002385"
     CDS             6134..6358
                     /locus_tag="IVK51_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431944.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRKQLVIESMQIRNPDHFTRSMSI"
     gene            6801..7007
                     /locus_tag="IVK51_002386"
     CDS             6801..7007
                     /locus_tag="IVK51_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP6431945.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7007..7258
                     /locus_tag="IVK51_002387"
     CDS             7007..7258
                     /locus_tag="IVK51_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431946.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEVLLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            7439..7711
                     /locus_tag="IVK51_002388"
     CDS             7439..7711
                     /locus_tag="IVK51_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431947.1"
                     /translation="MNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQ
                     ALELTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            8156..8335
                     /locus_tag="IVK51_002389"
     CDS             8156..8335
                     /locus_tag="IVK51_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431948.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            8394..8750
                     /locus_tag="IVK51_002390"
     CDS             8394..8750
                     /locus_tag="IVK51_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431949.1"
                     /translation="MLKTKKNSYKLKLSILSIICLLGMIISGHLYRVAYTQFIEYKVH
                     SLGLAGDRYVEYVHYSLKCSSYILLWLIFSLFELLNLIAISSYHLDKLNTKSSRYNEV
                     FKENVKLESDLNKLKK"
BASE COUNT         3484 a         1613 c         1995 g         2607 t
ORIGIN      
        1 gaactaaagc cgaaacatga acggcaaaat tttttagttg gagacgaccg tttagatcat
       61 gcggttgctt ttttatggaa agatccacag acaggagaga cagtaggggc aagttatcaa
      121 ggaacgatcg tcgattttaa tcgtttcggt aaacggggaa cgtataaaca cattgacaag
      181 aatcctacgc caaatcacgg atttaattta aaaataggcg atccgaagca tttaaaattc
      241 tttgaaagta gtattgatct gttaagttac gctgcattga atcgggagaa actacaagat
      301 gcctggttag tttcgatgga cgggttaaaa catcatgtaa tcagtcatta cgttgaagag
      361 tcgatttctg aattaagacg aaaacaaaca tttcctcaat cgattgaaat atgtgtggac
      421 aatgatcgtg caggacatat tttttatgaa aaagaacaga tgaagggaat cgttgatcct
      481 tttaccaata agaaaatccg ttgtgagcga ggaattccaa atgattggca agtacctaaa
      541 gaatacaaag ctacttatga agcagtcgca aaagaaatga gtgttgaacc tgaagcaatt
      601 atggccatac acaaaactga aactaattta cagctgacaa atcaattggt gtctgctcat
      661 gatgttcaat ctacgtttgg aaagatgttg gcgaagggcg aaccagtaga aacaattgat
      721 ttgaaagaag catgtaccac ggtcgcgaag gaactgaaag tatgtgaacg cgcagatgga
      781 acatacaact ttgatcgttt ttatagtaga aaagccaaca taaaagacgt taatgcagga
      841 atccttcttt cctataaagc agaacaatac tacaaaggct ataaaaagca tgaacatgaa
      901 tttgtacctg aagtgaaaaa ggattggaat gatcaattga agcatgagat ccagcaacaa
      961 gaaatcagaa agcaaaaacg tgcgatgttg tttcaacaag gcagacaaca agaaagggag
     1021 tgaagtcgtc actatgaaaa ccgtcatttt agcagaaaaa ccgtcacaag cgaaggctta
     1081 tgccgatagc ttttctaaag ctacccgaaa agatgggtat tttgagatcc aagatcgatt
     1141 gtttacagga gaaactgtga tcacgtatgg ttttggtcat ttagtcgagt tagattcgcc
     1201 cgatatgtac gatgaaaaat ggaaacaatg ggcattagaa catttgccta ttttccccaa
     1261 tcaataccac tatcatgttc ctaaagataa gaaaaagcag tttagtgtcg tcaaacgaca
     1321 gctgcagtca gctgatacga ttgtgatcgc gactgatagc gatcgtgaag gggagctgat
     1381 tgcctggtca atcattcaac aagcaggcgc caatcaagga aaaaccttta aaagactttg
     1441 gatcaactcc ttagaaaaag aagccattta tcagggattc caacagttac gagacgcaga
     1501 agagacctat ccaaaatttg aagaagtaca agcacgtcag atcgccgatt ggttaattgg
     1561 gatgaacggt agcccactgt atagtttact cttgcagcaa aaaggcatac caggaagttt
     1621 ttctttaggt agagtccaaa ccccaacact ctatatgatt tatcaattgc aggaaaagat
     1681 tagaaacttc caaaaagaac catattttga aggaaaagcc caagtaatcg ctcaaaatgg
     1741 cgcatttgat gcgaagcttg atccaaatga aacccaagcg acacaagaag catttgaagc
     1801 ctatttaaaa gaaaaaggtg ttcaattagg aaaacagcca ggtacgatcc ttcaagtcga
     1861 aacagagaaa aaaagtgcgg ctagcccacg tcttttttct ttatcgagcc ttcagtctaa
     1921 aatgaatcaa ctcatgaaag ctagtgccaa agatactttg gaagccatgc aagggttgta
     1981 tgaaggaaaa tatctaagct atccgcgaac ggatacccct tatattacgg aaggggaata
     2041 tgcgtatctg ttggatcact tagacgaata caagcatttt ttaaaagccg aagcgattcc
     2101 gactcctatt catacaccga atagtcggta tgtgaataat aagaaagtcc aggaacatta
     2161 cgcgatcatt cctacaaaaa cggttatgac agcagcggct tttgaacaac taagtccact
     2221 gcagcaggct atttatgaac aagtgttaaa aacaacggtg gccatgtttg cggaaaagta
     2281 tacctatgaa gaaacaacga tcttgacgca agttcagcag cttcagttaa aggcaatcga
     2341 aaaggtgccg ctagatctag gttggaagaa gttatttgga aaagagagcg acggaaaaga
     2401 gaaggaagaa gagccgctac ttcctaaagt gacaaaagga gaaatggtga cggtggatct
     2461 acaagttctt gaaaaagaaa ccaagccacc acaaccttat accgaaggta cgttgatcac
     2521 tgcaatgaaa acagctggga aaaccgtgga tagtgaagaa gcgcaatcca tattgaaaga
     2581 agtggaagga atcggtacag aagcgacacg ggcgaatatt attgaaacct tgaaacaaaa
     2641 ggaatatatc aaggtagaga aaaataagct cgtggtgacg aataaaggga ttttgctttg
     2701 ccaagccgtg gaaaaagagc cgctgcttac gagcgccgaa atgaccgcca aatgggaaag
     2761 ctacttgtta aagatcggtg aacgaaaagg aacacaaacc acttttctag ccaatatcca
     2821 aaaatttgtc tctcacttat tggaagtcgt accagggcaa atccaatcca ctgattttgg
     2881 atcgacgtta caagaagtga aagcagccag tgaaaagcaa gaagccgcgc gtcatttagg
     2941 aatttgtcca aaatgccagg aacaagaagt cttactctat caaaaggtgg cagcttgtac
     3001 gtctgaagcc tgtgatttta aaaggtcaga agggttcatt tgaagcaacg attgttttaa
     3061 aagaagactt cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa
     3121 gaacacgccg cactgcaaaa taatggaggg ttaatcttat gcgtaatacc acacacaaac
     3181 aacaagaaat cgaaaatcaa aaagccaaaa ttcgttcaga acaggaaaaa atgcgtgcca
     3241 gcaaacgaag actgcagcaa ttgaagaacc agcagaggat tcaaaaagaa atcgaattag
     3301 atcgataaaa tcttcctaac tattgtatac gtataaactt atgttatact gtttttataa
     3361 gtttatacgt atacaaagga gggagaaaat gacaaaagca atggtataca agctgctaga
     3421 agaatttaat ggaactttgg ctttgaagga tgccaaaaaa gcgggcattt ctcctgtaac
     3481 gatcaaacgt atggtagatc gaggggagct ggatcgagag tatccaggtt ttttcacctt
     3541 acctggtcaa tttcctgatg aattgtttat ggcgcaaaaa aaatatgaac gcggcattat
     3601 ttcacatatt accgcgttag atttgtatga tttaacagat atgatcccta gacagatcga
     3661 tctgactgta ccttacggtt atcacgtttc tgaaaaagga ttgaaggaat tcgcagttga
     3721 gctacactat acaaaagcgg aatggtatga attaggtaag attgaaataa aaagtcgcta
     3781 tgggaatcct attatagctt atgatccaga aaaaaccttg tgtgacattt ggaatccctg
     3841 gtataacgtt gaggatgaaa tcaaagtaaa agcaatcaaa aactacatgg agtcggacag
     3901 aaaaaatctt agaaaattga atgagtatcg acgaattttg cctacagata aaacgatgcg
     3961 ctcttatatt atggctttaa attaaaggag aatacacatg acacctacgc aattaaaagc
     4021 aaaagtgaag aatatttcta gagaaaaaga agttgatccg cagcttgttt taagacattt
     4081 tatgatggaa aaattcttgg aaaaaatctc agagtctcct tatcgtgaaa attttgtgtt
     4141 aaaaggcggc ttcttaattg gctcaaagta tggcattgaa aatcgaacaa ctaaagacat
     4201 tgatacgact ttacgggaaa tgaaagtaac caaagaaaca ttgacaacgg ttctaaacga
     4261 tattttttca acgccgacga aagaaggcat tcaatttgag attcaaggaa tgaaagaaac
     4321 gagggaagct gattattatc cagggtttag cttacgtgta ctggcacatt tggaaaacat
     4381 gcggccagat tttaaggtag atgttactac aggtgactcg atctatcctg cgacgatcac
     4441 tcacagtcat aaattgatgt ttgaggatcg aacgattcct ttggaatcct atcctacaga
     4501 acaaattatt gctgaaaaat taagtgcaac ctttgatttt ttaacagata attcgagagg
     4561 aaaagatttt tatgatctct atactattcc taaaatggag aagatcgatg agaagacctt
     4621 gaaagatagt gtgagaaata catttacacg tcgaggaaaa actgatccgt taaagatata
     4681 ctatgaaaag gatatggatg tactgaaaaa tagtcacgag ctaaaagata cctggaagaa
     4741 atatcaaaaa agcaatcctt atgctgcgtc gatcacgtac gatgagacaa tggattcgat
     4801 tagtaatttg atggaaaaag tgattcacgt cgaagaaaaa gagcgaaaac tagcttatca
     4861 aagacaccaa cagatgaata gagaaatgga gagataaaga tgattaataa tgtgacatta
     4921 gtaggacgct taaccaaaga tcctgattta cgttataccg caagtggtac tgcagtagcg
     4981 acttttaccc tagccgtcaa ccgaaatttc acgaatcaaa acggcaattg agaagccgat
     5041 tttattaact gcgtgatttg gcgcaaacca gcagaaacaa tggcgacatt agctaaaaaa
     5101 ggtagcttga ttggtgtagt tgggcggatt caaacgcgga cttacgacaa ccagcaagga
     5161 caacgtgtat atgtgactga agttgtagca gataactttc aactattaga aagtaaagca
     5221 gcgacagaga gccgtgcgca cgctgatcaa tcgtccacca gtccaagcac cgccaccttt
     5281 gaacaacgga atacagcgac gcctaacaac aatggattga acgcttcaca gaatccattt
     5341 ggtggacaat caatcgatat tagcgatgat gatctaccgt tttagtttga gaaagaagga
     5401 gtaagcatgg caaaaaaatt agtctgacga tggatctatt gatacccgta attgcattta
     5461 tagacgggat ttctttgggc gtcactgaaa atcctttggt aaatagaatt gcgaaaggat
     5521 cactatttct tgtattcgtg tatcttgtct gttcttatct tttaaaaaga gcaggaaagg
     5581 ataattcctt ttggcgattg actaaagtca cagtagcagc gacagctgtt atacttacgg
     5641 ctattctacc gctttatatg tagagttaac gggaaatcgt tgaacttctt gcgaaatatt
     5701 gctataatga acatgaaaaa gaggagttgc gctaacaacc cctcattaac agcaccgatt
     5761 aaagaacggt ggcagaatat tagttatttg gaaataaccg ctgacctgct aaagtagacg
     5821 gttatttctt tttgtcattt ttaagcatat ttacaatcaa actaatcaaa gcaatggtaa
     5881 acataccaaa accaagaatg gtttgaattg tctcatatgc ggacaaaagg cttactcctt
     5941 tcatagattt tagcacttac attcataagc accaccacct ttccagaagt cgccaccgtc
     6001 ttcaaacttt actgtcttga ctattatagc atatctaaaa tcgaataaaa attctcaatt
     6061 ttagctgttt ttgctataat tttagcgagg tgatcgtgat ggaacaatat attggtttac
     6121 tcgataaatt agggtgatca agacacagcc attgcttgtg cgatttacat tacgcacgag
     6181 tcatgaacca atcaattgtg tagtggccaa tatagaaata gcggataaat tgctgatttt
     6241 ggaagaaggc aagtatacta tttcagtgac aggtcacttc aataaacgaa agcaactagt
     6301 cattgaatca atgcaaatta gaaaccctga tcattttacg cgatcgatga gcatataaaa
     6361 gaagagctgc agtcctaaat gaggattgta gctctttttg ttttacatag aaacagtaaa
     6421 aatgaaaagg cctgtttagg aaggcacaag cgccttctca attgaaagac ctttctctca
     6481 ctccaaccgc caccgccgtt cgagaagaaa ggaagtgaac gctacgcgtt cactaagata
     6541 aagggtcttg catagcaaga caaaatagaa aaaatttcct ctcaccaaaa ggttcgctcc
     6601 tgttcgttat ctctttattg atttgtcaga ccggcaacaa gttgcctttt atcaccaaaa
     6661 agcgacaagg acaagggtaa atggcacgct gcgcgccctt gtcttgtcgc ttttcgttga
     6721 ttcattctgg ccaacaaatc aataaagaaa gaactaacga aaggaagtaa aacctttcct
     6781 aagaggaggc agaatcaatc atgcgaaaga aatttgatta ttggggtgtt cccttcagcg
     6841 aattattccc aaactatcat gcaccacata ctgtcgaatg cgattgtgga gaaagagcga
     6901 aatgtatcaa aagctattgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt
     6961 cttatggcga ttatatttta attgatgaga aaggaaagaa acgctgatga aaacacaaca
     7021 caaattattg actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga
     7081 aaaagtagca gaagttttac ttattccgcg gtactggacg atagaaaccc ctttagtaga
     7141 ggatgcaccg actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta
     7201 tcaagaaaac ttggaaactt atgaaaacga atattggtta accaaattag agaactaaaa
     7261 aggagaaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
     7321 aatggcacaa aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaaaaa
     7381 acattataaa gaaaaattgt cggtagctgt ggaagaaatg attgaataag gtgtcgaaat
     7441 gaatcttatt gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg
     7501 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta ttaaaccttc
     7561 tgaataccaa gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac
     7621 tgtttcaagt gaatggttag attattttga aaatatcaat aagtatggcg catattacac
     7681 catgaaaaag gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca
     7741 tatcaacgat ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaatgtg
     7801 tgaaacaagg acaaaaaacg cctagtaact aaggaaatgg ttcttatttc gcaccaatcg
     7861 ctccaaccgc ccccgccgct taggtggtgc gaaataaacg ggcaaagccc gataacttaa
     7921 gagaaagaag gtgatccatt tgaacaagat tattccctta ttgttagtac tgtttgttgg
     7981 cgaaatactt atgcgagata gtgcagctgt acagttaaac ctcgctgtat ttttggtaat
     8041 actggtgttt tgtttgatct tttggcggtt tgtattagtc ctcgtgagtc tactattcaa
     8101 gatgattgca gccatatttt tcatagggtt atttcgatga aaggagtgag taccaatgcc
     8161 aattgcaata ggaaataaac ggttacccgt gacattagat gaaaagagac aaaaagaatt
     8221 gcagcaacta aagcagaagt atggcaaaag tgaatctaag attatgtgta ttgcgttaga
     8281 tctattgatc gcgcaagaaa aagcaggatt tgatgtacca gcgctaaaaa agtgacgtca
     8341 ctttttaatt tgatctatat aagtgatatc acttttacca aggagggaat taaatgttaa
     8401 aaactaaaaa gaattcgtac aaattaaagc ttagtatact atctattatt tgtcttttag
     8461 gcatgataat tagtggtcat ttgtatagag tggcatatac gcagtttatt gagtacaagg
     8521 ttcactcttt aggattagct ggggatcgat acgttgaata tgtccattat tcattaaaat
     8581 gttcttcata tattcttctt tggcttattt tttcattgtt tgaattgcta aatttaatag
     8641 caatatcttc atatcactta gataagctaa ataccaaatc tagccgttat aacgaagtgt
     8701 ttaaggaaaa tgtgaagtta gaaagtgact taaacaagct aaaaaaataa aaagaagaaa
     8761 acataagtta actaaaatta ttttattaag aatgatggaa tataaggata aggttaccaa
     8821 cataacgatc attacttctc tgatatgcta tggacttcta tcaatcagta ttttgtctat
     8881 agattatgga gtaactatac tgcaagcctt agttttggct atgcctatgt atctatcttt
     8941 gtttatcatg catgaaatat ataagctatt tgttttatca aaaaatgtag aaatacgggt
     9001 aaatattttg gtgaatagtt atcaaaaaga actcaaagaa agaagacggt ggcggtattt
     9061 attgaaagga tgaaaaagta ggatttgaca tactggtatt cagaaagtgc cgtcactttt
     9121 aaaacaatgg aaaaggtgga gataagatga agaaaaaaca aactaaacta tcattagcag
     9181 atatttaaca cagttaaccg caactgaaga tggtgttgtt gaatttgaac aatctgaaat
     9241 tgcggtagta gatgatcgtt accttaaaat gccttacttc tttgaccaag caaaggtcat
     9301 ttgcctttgt cactacgatg gggtacgtga ttttaggcgt taaaaagtag tgattatatc
     9361 aactttacga gtaaatataa atttagacat gaaactgaat tatccaatgg aaaatcatgg
     9421 tttataatct agaaaaatta tagtaatgat tggattatat atattctttc gtattttcgt
     9481 acaactgtag cttgtcaaaa ttgagaaatt taaccgatta ggtactttac atcgttattt
     9541 gggtaatttc taaaacatga agcttaccaa cgaataaact gagactatct tattaattga
     9601 tcaatataag aaaatatctt tgaggagatg atgtactatg ttttcgtcta tccacgattg
     9661 gttaggtggt cttttcggcg gtggaggtaa aggttttta
//
LOCUS       DACCDF010000093         9317 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCDF010000093 DACCDF010000000
VERSION     DACCDF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.097"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..2122
                     /locus_tag="IVK51_002391"
     CDS             <1..2122
                     /locus_tag="IVK51_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431950.1"
                     /translation="KKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQYK
                     DDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKY
                     GTPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFD
                     TIKDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTL
                     SIYHLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYW
                     ERTTNLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENN
                     KEFFIGQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYI
                     LVPKKYKEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSF
                     NTLYGKAKDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQIN
                     PYIKEFNLENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVS
                     FTINSKKIALKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIF
                     LINLITSIIIYKKNSFKYFKENIL"
     gene            2119..2739
                     /locus_tag="IVK51_002392"
     CDS             2119..2739
                     /locus_tag="IVK51_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6431951.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            3432..3812
                     /locus_tag="IVK51_002393"
     CDS             3432..3812
                     /locus_tag="IVK51_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP6431952.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            3794..4708
                     /locus_tag="IVK51_002394"
     CDS             3794..4708
                     /locus_tag="IVK51_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6431953.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            4948..5637
                     /locus_tag="IVK51_002395"
     CDS             4948..5637
                     /locus_tag="IVK51_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431954.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKI
                     EEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5758..5844)
                     /locus_tag="IVK51_002396"
     CDS             complement(5758..5844)
                     /locus_tag="IVK51_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP6431955.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            6489..7226
                     /locus_tag="IVK51_002397"
     CDS             6489..7226
                     /locus_tag="IVK51_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP6431956.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            7254..7793
                     /locus_tag="IVK51_002398"
     CDS             7254..7793
                     /locus_tag="IVK51_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP6431957.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            complement(8291..8752)
                     /locus_tag="IVK51_002399"
     CDS             complement(8291..8752)
                     /locus_tag="IVK51_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6431958.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFS
                     FTHGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
     gene            8885..9259
                     /locus_tag="IVK51_002400"
     CDS             8885..9259
                     /locus_tag="IVK51_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HAP6431959.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
BASE COUNT         3601 a         1415 c         1450 g         2851 t
ORIGIN      
        1 aaaaaaaata aacgctgttt tatcactttt tgtctgcatt acaatactat tcttttctac
       61 taatgttata aatggtataa agacttctga agcgctatat aaaaataccg ctgaaattca
      121 tatgcaatat aaagatgata tggactttaa cagttatata aacgctttgt atgaaatatc
      181 tacacaagaa aatattaata tttctcaata tagttttact gctgttaacc aactaactat
      241 tcttgctaca aaccctaacg ctaacaaaca ctggaaattt tctaaaaaaa atatattagc
      301 aagaaacgat tctaaagtcc attatactaa taacaaaaat tctaagtacc aattaaaact
      361 tccaaataat tttttaaata taaatattta tcctttttct catgtggaaa atattggatt
      421 aagtgaaata ttatatgtac aaggaaactc aaacaaatta attcctgtac tgaaaaaata
      481 tggaacacct actatttcca atatcaatca atcagatact tttcaaatta atactaatat
      541 tgttcttgtt atctgctatc ttagtatatt tattgtaatt actacaatta tatttgcttt
      601 ttcaaaaatg aaagaaatca ctctaaaaaa aatgttaggc tataactctt ttgatactat
      661 aaaagattct attaaagagt taaacctctt attttgttct ttgattataa ctttcatttt
      721 atctattttt ttgagcatat attacagtaa cgaaaatttt ttcctatcat ttcttctatg
      781 tagtattttt cattttataa ttagtttttt atttatcata gttactctaa gtatctatca
      841 tttatctagt ataaaaaatt atctaaaaaa ttcacgacca cttaagttaa atctttgtat
      901 attaaatatc tgtctattct taagcatgat ccttctgtta atagcttcta caaaagtaat
      961 aaatatccat aatgaagccg aaaataattc tcttaaatat tgggaaagaa ctactaattt
     1021 atataaaaca aatattacta atcaattaaa tagaaacaac accgtagaag aaaataacta
     1081 cttgaaaaaa gctagtaaat ttgtttataa aatacagaag aattataaaa cttttattat
     1141 cgcgccatac aattacgcaa ctatccaaga aaataataaa gaatttttta ttggacaaaa
     1201 agtttatcca aattttgaag actatgttag tcaacctgct ggtgcaggaa tatgtgttga
     1261 tttaaactat ctaaaatatt acaatcctat ttcttttgaa gaatctactg acttaaattt
     1321 aataaactct gatccactaa ctacttacat tctagtacct aaaaaatata aagaatatgc
     1381 aaaaatgatt gaaaaaaatt atttagaaga catacagttt agacttgaag actctcctcc
     1441 tcaagctcct tataaaactc ctaatttgaa aaatttaaaa ataaaactaa tatttgttaa
     1501 taataaccaa aagtatttta gttttaatac cttatatgga aaggcaaaag ataactatac
     1561 aataactgat cccatagtta gagttattaa tccagaaata acagaatcat tattttgggg
     1621 aaatatattg actagtgatg gtggattatt ttttgatcag aaacatactt caaaccaaaa
     1681 cttttttaat caaattaatc cgtatataaa agaatttaac ctagaaaata tcataaattt
     1741 ttcttattct gtttttcaag aaactggtga attattatct caaaaaaaag taaataaatt
     1801 aaatgcatat ctacaatatc ttttaatatt aggattattt ataataatta attttcaaat
     1861 gatttttgtt agttttacta taaattcaaa aaaaatagct ttaaaaaaaa tatttggata
     1921 ttcaccaata agtcaagttt tagatttatg tttactccct tttacaatag agttatttgc
     1981 tactatgtgt tttcaattga tattttcaaa aaacacttac ttgtatttta taatttttat
     2041 gatcttttta ataaatctaa ttacttctat tattatttat aaaaaaaata gttttaaata
     2101 ttttaaggag aatattttat gatattatca gttgagaatt tcaccctgtc aattaataaa
     2161 aaaaagatca aacaaaattg gaacttttca atatcagaaa aagattttat tatagtaaca
     2221 ggaaaaagtg gtagtggaaa aactactctg ttaaataatt taagcctaat agataaacaa
     2281 tataccggta acttattcta ttttgaaaag aaagtttcta aacatgatat acaacgtcta
     2341 cgaagaaatc atattagcta tttatttcaa aactatgctc ttcttgaaaa tcaaacagta
     2401 caatacaatt tcgaattagc aaaaaagttt aacagtaata tttccaatga tcatatttat
     2461 aatttattaa cagatttttc tttgccaaaa gatatcttaa aagaaaaaat ctttctctta
     2521 agcggtggag aacaacaaag aatagcatta attagaaata tgttaaaacc atttgatata
     2581 ttatttgcag atgaacctac aggaaattta gataaagaaa actcaacttt tataatgaac
     2641 tttctgaaaa atttaacaga aaaagaaaaa aaagccgttg tactagtaac acatgactta
     2701 aatgttctaa actatggaac taatattata gaattataat ttttacccgt gatgctagct
     2761 gatttaaaac tagcaccacg ggtatttatt ttatattcca tctctcctat cccaaaattt
     2821 aaataatcac ttaataaaaa ggaggcaccc aagtgatttt attgaaaaaa gagtatgaaa
     2881 aattttatga tagacttatt tattctttgc aattgccaaa aaaattaaac cgtcaattac
     2941 aaaagactta tgaaactaaa tcaactgaag aatgggagag ttattgctag atttttggta
     3001 gaagtaacaa aaagtggaca tattatcata atgactcgcc gaaagtctga taaaaaattg
     3061 gctaaaaata ttgctgaatg tttaataaaa ctatcagttt tagctaaata gttagtagtt
     3121 tacacaaaaa agcatttatg ttttttgttg caaagcaaca atttcatctt cggtgaaata
     3181 agcgaaactt tacaaaaagt ggctagcatt tgctagcttt ttttgtattg tggagcgtgg
     3241 cttttgaggg ggattcaagg gggagcttgc gcccctttga caagcggtta tttgcaccaa
     3301 atacggaggt atttggctag caaatataga gcttgccaaa cccttttagt gagctttact
     3361 cattgtacct ttttaggtac aatggtattg cgaacgatac gtccgtagga cgtactgaaa
     3421 gggtgggact tatgagcgaa caaaatcaaa atttggctag caattcttct aaaaaatata
     3481 cctatcgatc cgagccaaaa caaatcagtt ttcgagtgag cgaatccgaa tttgcaaagc
     3541 tgaagcaatc ggctgaagct ttgcaaatga gtgtgccggc tttcgtgaaa gccaaggcac
     3601 aaaacgcgcg tctggtgaca ccgaaagtgg cgccagacat tgcccaggca atcgcgcgcg
     3661 atttagccaa agctggaggg aatatcaatc aaattgctaa gtggtgcaat acacaccaac
     3721 atgacgttgc ttctggtgac gctcaacgtt tatccgaaaa cctaaaaatc atgcaaaagg
     3781 agctacaaaa gatatggcaa caattaaggt aagccgaacc acttcttgta gtcgtgcaat
     3841 taattacgca gaaccacgag caaccattaa aacaggaata aactgtgacg tcgattatgc
     3901 caaaagtgaa atgaaacaaa ttcgcatgct gtatggaaaa gacgaccgtg tacaggcaca
     3961 cttattgatt caatcttttc gaccaggaga aattaccgct gaaaaagcta atcaactagg
     4021 ggcagagtat gcaaaaaaaa tagcccccga tcaccaaata gctatttaca cacatacaga
     4081 caaagaccat attcataatc acattgtcat taactccgtt aatctggaga ctggaaaaaa
     4141 atttcaagct catggtcaag catttctaaa caaatgttat gacctaaatg acgagatttg
     4201 tcttgcacat aacttaccaa tcactgagcg aggaaagaaa ccagaaaaac gaaccatgtc
     4261 tgaaataaaa ttaaaagaaa aaaatgaacc tgtttggaaa gacgaaattc ggattgcgat
     4321 cgatcaaaca atgaaaaaca aaaaaaccag aacctatgac caattttgta attccctcaa
     4381 aacattgggg attcattgct tcaatcgcgg gaaaaatttc acttacgaat tgatccacaa
     4441 aaagaaaaaa gtacgatcca acagactagg aaaggactac gaaaaggaga cgattttaca
     4501 tgagctggac agacgagaac gaacctataa ccaacagcga accaacgagc tacgaagagc
     4561 tcgagctagc attcgcgaaa ctgcagaaga agtacgaaga gaacaacgaa ctcttacgcc
     4621 tgaacaaccg gaaattagac cggctcgaag aaaccatgaa caacacgttg aaagaggaac
     4681 agaccaccaa caagaattat cgctctaaaa cgttaaaaga catagaaaac gaaataaaga
     4741 aacaacgtgc ttttctcgtt ccttatacgc aagaaaggat acaaactacg attgatttac
     4801 aagttaaaaa actccatcaa gagctattag accagcgaaa taaggaacag aaaaaggtgc
     4861 ttattcaacg atcattggat tttcttgccc ccttatgttg cttagtcctg acactgttcg
     4921 cattttggtt agtatgggga ggctaaaatg gcattttatt taccctatct actcattttt
     4981 gttagtattt caggaagtat ttggctcatc tataaaattt ttcaaacaag atattcatta
     5041 aaaggctcta aaattcgatt taagcgattt ttctttcttg gctgtatatt ttccttgata
     5101 atcgtttcaa gcggcttatt cggcgtttta gagggaaata agcgcgtttc tagaagtatt
     5161 ttattaggaa atgtcactca aaagtatgaa tcagcgcgtg ataaaaagaa aaaagagcaa
     5221 gcattagcac aaaaaatgga agagtttacc acttgttatg aggaaatgaa cgatattttt
     5281 gtgaatcaag aaaaacgttt gacggataaa aacatggaaa aactcactcg tctttatcaa
     5341 aacttgccag aaaaacagca aaaagaggtt caagacaact atgagcgaac caaaaaagac
     5401 gtgcaatatg taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca
     5461 aacccttggt ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa
     5521 cgctatgaaa tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc
     5581 gccttaaact atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt
     5641 tgatttaaaa tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc
     5701 ccagagaggt cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta
     5761 cttcttgttt cggttattca accaataact gaataaagag acacaaactc ctacaagaag
     5821 ggggcaaatg aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat
     5881 aggcgacaag ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt
     5941 gttttagggt tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag
     6001 ggaaattaat caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc
     6061 ctctttgaac aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag
     6121 catagcgatc acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc
     6181 gcgaaccttt ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta
     6241 agatatactg ttagatatac tgttagatat actgttaata ataactgtta gggattagca
     6301 accttatggg agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg
     6361 gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa ttgtcacact
     6421 atgggggata aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt
     6481 gatttttatt gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa
     6541 gaaaatttac ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa
     6601 aaggtacaaa agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata
     6661 taaatgcacg tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta
     6721 ctttgtcttt tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat
     6781 ttaaaatcga taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata
     6841 tacttaattc aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa
     6901 agagtagcta cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta
     6961 aaaaaggtca ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa
     7021 gttatcgcat gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa
     7081 acgttaattc aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct
     7141 acaaaggaaa taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta
     7201 ccttagataa ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac
     7261 ccaaagaaac ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa
     7321 tctctaaaca tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata
     7381 aggttgtatt acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac
     7441 tatataggtt gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa
     7501 acgtgattgt aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac
     7561 aactttccct caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac
     7621 agctcaccct acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt
     7681 atacggaaga aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga
     7741 tagaaaaaca cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg
     7801 gaacattcat gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct
     7861 tcagtccgat gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga
     7921 atccaaatta ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat
     7981 aaaaaagact gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa
     8041 gcacgcgctt ataaagcgct accaaaattt tatcatattt tataaaaaat aacaatccag
     8101 taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaactaca gaaaatcaag
     8161 ccttaaaaag agctttttac ctagaccaat taaaaatcac aaagtgagcg tctctatcca
     8221 ttggtagagg ggctacttgg catagtgtac attatgtcaa ctatgcaatt aaataactaa
     8281 ctacttcata ttagcttaat aaaataatca aaatcactag gggaaaaaat attgtataaa
     8341 aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag atatctatta
     8401 ctattaatcc agatagagca aaccaagtaa atattccgtg tgtaaagcta aaaaaattca
     8461 tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata atagctgtca
     8521 ataccagtaa attattttgt ttatttttta aattatcttc atttaaaata gaatttatgt
     8581 tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt gttttgttat
     8641 tttcccactt tgaaatagtt tgacgtgtaa cattaaattt tcttgccaca tattcttgag
     8701 tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc attttttctc
     8761 ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg ccaaaaaagt
     8821 tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat aggagaaata
     8881 agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa ctagtggagg
     8941 aatagttagt gctacagaag ctttaaatct agatgtaccc gaagaccata ccgccattta
     9001 cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga gatatgctgc
     9061 tggcggcgga gatttcaact gtggggttaa tggatttaaa gtttatgcaa attactacca
     9121 tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag tacgatcagt
     9181 tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg gaaatactgg
     9241 ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta catttgaaag
     9301 gaaattcaaa atatgtt
//
LOCUS       DACCDF010000094         9154 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCDF010000094 DACCDF010000000
VERSION     DACCDF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.098"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            806..1006
                     /locus_tag="IVK51_002401"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IVK51_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IVK51_002402"
     CDS             1493..1672
                     /locus_tag="IVK51_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431960.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IVK51_002403"
     CDS             complement(1718..2323)
                     /locus_tag="IVK51_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6431961.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IVK51_002404"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IVK51_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IVK51_002405"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IVK51_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IVK51_002406"
     CDS             3358..4329
                     /locus_tag="IVK51_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6431962.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICKENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IVK51_002407"
     CDS             4469..5782
                     /locus_tag="IVK51_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP6431963.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQISGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IVK51_002408"
     CDS             5825..6691
                     /locus_tag="IVK51_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP6431964.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLMPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IVK51_002409"
     CDS             6688..7536
                     /locus_tag="IVK51_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP6431965.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IVK51_002410"
     CDS             7550..9022
                     /locus_tag="IVK51_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP6431966.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..>9154
                     /locus_tag="IVK51_002411"
     CDS             9039..>9154
                     /locus_tag="IVK51_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6431967.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         2920 a         1525 c         1871 g         2838 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg taaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaaatttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaatgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgtgttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggaaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttttaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caat
//
LOCUS       DACCDF010000095         8903 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCDF010000095 DACCDF010000000
VERSION     DACCDF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.099"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            250..936
                     /locus_tag="IVK51_002412"
     CDS             250..936
                     /locus_tag="IVK51_002412"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP6431968.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            956..4537
                     /gene="smc"
                     /locus_tag="IVK51_002413"
     CDS             956..4537
                     /gene="smc"
                     /locus_tag="IVK51_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP6431969.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            4546..5355
                     /locus_tag="IVK51_002414"
     CDS             4546..5355
                     /locus_tag="IVK51_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP6431970.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMVHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            5367..6365
                     /gene="ftsY"
                     /locus_tag="IVK51_002415"
     CDS             5367..6365
                     /gene="ftsY"
                     /locus_tag="IVK51_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP6431971.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            6552..8819
                     /gene="gshAB"
                     /locus_tag="IVK51_002416"
     CDS             6552..8819
                     /gene="gshAB"
                     /locus_tag="IVK51_002416"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP6431972.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
BASE COUNT         3159 a         1564 c         1961 g         2219 t
ORIGIN      
        1 acttctccaa taaacggtgt tcaaagtcca gtttctcggt attaagtcaa atttatgcaa
       61 aaatatggaa agccattttc acaaaatttt ttcttcatac tcgaaactaa acgaattttt
      121 cacaacttct cgtcaatcac taggattatt aaagctttac ttaccttttg accgaaccat
      181 ttcaaatttg acaaaaaacc agtataataa ggaagtatcg aaaaaatatc cataaaagga
      241 atgtggaaaa tggacaacca gctgacaaaa gaactaaaag aaaactataa catcgttttt
      301 cacgatctaa atttgcttga acaagctttt acccattcat cctatgtgaa tgagcatcgc
      361 aatctgcaat tatcggataa tgagcgactt gaatttttag gagatgccgt tttagaactg
      421 atggtttctc agtatttatt ccgtttatat cctcatttgc cagaaggaaa attgacaaaa
      481 acacgagctg cgatcgttcg cgaagacagc ttgtcgaaat ttgccaaaga atgtcacttt
      541 gatcaatatg ttttgttggg caagggagaa gagaattcag gaggacgtac gcgaccagca
      601 ttgctttgcg acctattcga agctttttta ggcgcgttat atcttgacca gggattcgac
      661 acgacccatg cattcatcga aaaagtgatt tttcctaaag tcaaagcagg tgctttttca
      721 catgagatgg atcataaaac aaaactacaa gaagtcttgc aaaaatcagg tgatgtatca
      781 atcgaatacc gattgatcaa tgaagaaggt ccagctcatg aacgagtatt ttggatcgaa
      841 gtttatgtag atgatcaatt gattggcact ggacaaggaa aatcgaaaaa attagcagaa
      901 caagctgcgg cggaaaatgc attggcagca ctgtaaaagt aagtgaggga aagctttgta
      961 tttgaaacga attgaaatag ccggttttaa atcatttgca gacaagacag tcattgactt
     1021 tgaaaatagc gtcacagcag tggttggacc aaatggcagt ggaaaaagca atattacaga
     1081 agctatccgc tgggtattag gagaacaatc tgctaaaagt ctgcgaggcg gaaaaatgcc
     1141 ggatatcatc tttgccggtt ccgatacccg taagcagctg aatattgcag aagtcacagt
     1201 gattttagac aatagtgatc attatctgcc attggagtac aatgagatca gtgtaacccg
     1261 ccgttatcga cggactgggg aaagcgagtt tttcatcaat aaacaaagct gcagattaaa
     1321 agatatccaa gaattgttct tagactctgg attaggaaaa gaatcgttct caatcatttc
     1381 tcaaggaaaa gtagaagcga ttttcagtag taaaccggaa gaccgtcgtg ggatctttga
     1441 agaagcagct ggtgtcttga aatacaagca gcggaaaaag aaagcagaac agaagctttt
     1501 tgaaacagaa gacaacctca gccgcgtcca agacatcatc cacgaattgg aagaacagtt
     1561 aactcctttg gccgcccaga gtgaggcagc aaaggagttt ttacggctaa aagaaacact
     1621 gactcaaacc gatgtctctt tgatggttgc cgaaatcaag acagcaaaaa aagattggga
     1681 caacaaacaa gcacagcttg cgaaattcaa tctagaatta ggcaaactat ctgagtcgat
     1741 ccaagaacaa gaatcaattt tggcgaagca gcgtaaagaa aatgcgcaag ccgatcgtct
     1801 gatcgaaaaa aatcagcaag tattattaga tctttcggaa aaactgaagc agacagaagg
     1861 acaaaaagat gttttgcagg agcggacaaa gcatacacaa aaaagctcgc aagaatatca
     1921 aacttcatta gcagaagcac aaaaaaaggt taaacatttt gaaaagcttc aagaaagctt
     1981 gttgaaagaa gtagcagaaa aagaaacaga aatccaaaaa gctgaagcaa gtctcataaa
     2041 aacacagcaa gaattagaaa aataccaaaa atcaacgaaa gaattactgg cagaactaag
     2101 agatcaatat gttgatttga tgcaagagca agcagctgta ggaaacgaac tgaagtatct
     2161 agaacgtcaa tatgtacaag aaacagcaaa aagtaagcag acgttagcca agcaatcgga
     2221 agtagaggca tcagtagacc gattgatctt acaaaaagaa gaattgacac aaaagcaggc
     2281 gcagctaaaa tcatctttaa ctgaaacaaa agagaagtta gaaatgatcc aacaaaacgg
     2341 gaaaaagttc caagaagcat tagcaaaaga acagcctaaa atgtatcaat taatgaatca
     2401 agtccagcag ctacgtgctc gtaagaaaag ccttcaagaa atccaagaaa actattttgg
     2461 tttttaccaa ggggttcgat tggtcttgca gcataaacag cagctttcgg gtattgtcgg
     2521 agcagtggct gaactgatcg atgttcctgc agatttcacg ttagctatcg aaacagcttt
     2581 aggtggggca gcccagcatg tcatcgttga aaatgaaaaa gatgctcggc aagccatcac
     2641 gtatttgaaa cagcaaagag gaggaagagc aacattcctt ccactgacga caatcaaacc
     2701 aagacaatta ccggctcata tattgacaca agcagcagct gtagaagggt ttatagggat
     2761 tgccagtgaa caagtcagtt atcccgatca aatccaaact gtggttcaaa acctgctagg
     2821 aacgatcctt ttagccaaag acttaactag tgccaatgca atcgcgcaaa cgatccgcta
     2881 tcaatatcga gtggtttcct tagaaggtga tgtgatgaat gccggaggat ccatgacagg
     2941 tggagcgaat aaacgaggaa accaaggcag tttgtttgta caaaatcaag aattgaaaca
     3001 gttgactagt gaatttgaag aagcagataa acaactccaa gcacaagaaa aaaaagttca
     3061 ggagctgcaa caagaaacag ctcgtttagc cgaagaacaa gaagttttgc gtacacgagg
     3121 cgaacaatta cgttttgaag aacaagaagc gacaaatcag ctgcaaaaca tcataaatga
     3181 acttgaacgt tttgaaaaag aaaagcaaat ctcaactttt gagacacgtg aactgcagca
     3241 gtttatcgaa gattacgaaa agcaaacgaa cgaattgaaa gacaaacaaa cagatcttga
     3301 atcgcaacga caaaaaatcg acgaagaaat caaatcgtta agtcaagaaa gcgaccagat
     3361 ggaagccaga agagcacaag tacaaagtca aaaagcacaa gaacaagcag atcttgctgt
     3421 cttgaaagag cagtttaatc atctgcagat ccagctccgc ggtgcccgag tccaaaaagc
     3481 agaggcaacc gaacgtcaag aagcgataga aaagcagttg gcgacattga cagcagactt
     3541 ttctgatcat gaagtcacag aagaaagttt ggaaaaacaa attaacgaat tatctgctca
     3601 gcgagaaaca ttgaaagccg agttggcaaa agccaaagag cagcgagata gaaggcaaaa
     3661 ggagatcgat aagcttgaag cagtcttggc agagcgtaat cgtgagcaaa aagcacgact
     3721 gtcggaacaa tccaaattag aagtccaaaa agaccgagct gaaatgattt tagataatca
     3781 tttgacctat ctgcagtcag aatatcagct gacatttgag aaagcttccc aagactatca
     3841 agaaacaacc gatatcgaag acagtagaac aaaggtttca tccttaaaag aacagattga
     3901 aaaactggga ccagtaaact taaattcaat cgaacaatac gaacaagtca gtgaaagaca
     3961 tacattctta gcaacacagc gagatgatct gttagcagcg aaaaaccaat tgtttgaaac
     4021 gatggatgag atggatgatg aagtgcggac aagattcaaa gaagtatttg aagctatccg
     4081 tcaagaattc aaaattgtct ttccaaatat gtttggcggc ggtaaagctg aattagtact
     4141 gacagaacca actgatttgc tgaaaacagg tattgagatt gaagtacaac ctcctggcaa
     4201 aaaactgcaa agtttaagtt tgctttctgg aggagaacga gcacttacag ccatcgcgtt
     4261 gctcttttct attatccgtg tctgtcctgt tccgttttgt atcttagatg aggtagaagc
     4321 agcgttagat gaagccaacg tgaaacgttt tggtcgttac ttgagcgatt tccaagatga
     4381 cacacaattt atcgtagtca cacaccgcaa aggaacgatg gtcgcagcag atgtactgta
     4441 cggagtgacg atgcaggaat ccggtgtatc aaaaatcgtt tctgtacgga tggaagatat
     4501 caatgaagag gcgaaaatca acgaaacgaa agtgtaggaa cgaaaatgat caaattagta
     4561 gcaatcgatt tggacggtac attattaaat gaacaaaagc tgatttcaga tgagaataaa
     4621 caagcattag cacaagcaaa acagcaaggg gtcaagatcg ttctatgtac aggacgcccc
     4681 ttagctgcga tggtgcatta tctgcaagaa ttaggactgg tagatgaagg tgactacagc
     4741 atcactttca acggcggttt ggtacaaaaa aatgatactg gtgaaatcat ggaaaagaaa
     4801 gtgatggaag tatcagatat ccaacgctta tacaaactgg ctcagcaatt ggatctgcca
     4861 ctagatgttt tgtctgacaa tgtggtttta cagctgccta gtgccccaag gaagcaatcc
     4921 ttgtacaatg tgttgaataa gctgctgcaa ttccaaccag cggatataag cgatattaca
     4981 gatgaatttg tgcttaataa ggcagtaatc gcttatgacc aagaagaatt agatcctaaa
     5041 atcaaagaaa taccagcaga attccatgaa atgtatgaga tcatcaaaac gagaagtatg
     5101 cttttggaat ttatgccaaa aggtgttaca aaagcttatg gtatttcttt attggcaaaa
     5161 gatcttggtt tgaaacaaga agaaatcatg gcaatcggtg atgaagaaaa tgatttaccg
     5221 atgatccaat acgctggact aggcgtagcg atggagaatg ctgtgccttt cgtcaaagaa
     5281 gcagcagatt tcgtgacagc cagcaatgaa gaaaatggcg tagccaaagt gatccaaaaa
     5341 tttgtattag agtagggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
     5401 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
     5461 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
     5521 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
     5581 cagcgatacg aatcaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
     5641 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
     5701 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
     5761 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
     5821 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
     5881 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggcggcgatc
     5941 cagcagctgt cgtattcgat gctttggaaa gagcaaagat agagcaggca gatgttttac
     6001 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
     6061 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtactag
     6121 atgcaacaac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
     6181 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
     6241 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
     6301 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
     6361 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
     6421 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
     6481 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
     6541 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
     6601 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
     6661 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
     6721 cagattttag cgaaacacaa acagagatga tcactcctgt taccgattct attcccgaat
     6781 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
     6841 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagcg atcattattg
     6901 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
     6961 agcgaaaaca gatggtaagc ggtatccatt tcaattttga atttggcgat gagttgctaa
     7021 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
     7081 atttgaaaac ggcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
     7141 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
     7201 gcagtattcg taacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
     7261 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
     7321 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
     7381 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
     7441 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
     7501 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
     7561 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
     7621 aagggatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
     7681 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
     7741 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
     7801 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
     7861 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
     7921 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
     7981 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
     8041 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
     8101 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
     8161 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
     8221 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
     8281 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
     8341 ttattggtga cggtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
     8401 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
     8461 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
     8521 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
     8581 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
     8641 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
     8701 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccctttgcag
     8761 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
     8821 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag cttttttgca
     8881 tgtaaaaatt tatgtgtttt ttc
//
LOCUS       DACCDF010000096         8812 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCDF010000096 DACCDF010000000
VERSION     DACCDF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.100"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            349..2391
                     /locus_tag="IVK51_002417"
     CDS             349..2391
                     /locus_tag="IVK51_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6431973.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2402..3022
                     /locus_tag="IVK51_002418"
     CDS             2402..3022
                     /locus_tag="IVK51_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP6431974.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3034..3492
                     /locus_tag="IVK51_002419"
     CDS             3034..3492
                     /locus_tag="IVK51_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6431975.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3511..3789
                     /locus_tag="IVK51_002420"
     CDS             3511..3789
                     /locus_tag="IVK51_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6431976.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3814..5181
                     /locus_tag="IVK51_002421"
     CDS             3814..5181
                     /locus_tag="IVK51_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6431977.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5196..6341
                     /gene="dgoD"
                     /locus_tag="IVK51_002422"
     CDS             5196..6341
                     /gene="dgoD"
                     /locus_tag="IVK51_002422"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP6431978.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6368..6859
                     /locus_tag="IVK51_002423"
     CDS             6368..6859
                     /locus_tag="IVK51_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431979.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7302..8501
                     /locus_tag="IVK51_002424"
     CDS             7302..8501
                     /locus_tag="IVK51_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6431980.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
BASE COUNT         2738 a         1442 c         1867 g         2765 t
ORIGIN      
        1 acacaaaatt attgacgctc ccaaaagcat tgtttttagc agtttaatat tattcttttt
       61 caattagact gacttgattt agttacggaa tagatacttt aatgtgttga agaagcaacg
      121 tcccgtgttt atttactaaa gcatccaata ttctaccgaa tatttaatat tcacattagc
      181 cctaaaataa ttgtcatacc atgttacatt tgatataaca gcgttcattt taaaaatagc
      241 tgttagtgtg acaaaacatt aaaccaagtg tgacaaaaaa cgtgatgaaa gctttccgga
      301 aacgtatgtg aacaagtata taatgatttt gtcaggagga tagtcaaaat gaataaacga
      361 attcgtgaaa tactcaaact gattattaaa aaccctgaaa tgaaactttc cactttaaca
      421 agtgaacttg atttgacacg tcgtcaaatc aattacgcga tcaatcaatt taatgaagaa
      481 ttgaaaacaa agaacattcc gttaattcag agaagtcatt ctggaaatat tacggtacca
      541 atcgaggtta tccaaatgat atcacagcct gattacgaca taggtgatga tcaggaactt
      601 gtcgttctca ctgatagtga aagacaagca ttgatagtga tgactctgat cgctaatatc
      661 gattacatca gtctggatca tttggtagat gcggttgatg ttagtaagac gactgttatg
      721 gaagatatca aacgaacgga accgttcatc agaaattata gtctgacaat tcgatataat
      781 cggatgaatg gttaccaatt ggttggttcg gaacaccgta tcttacagct gttatcggat
      841 ctagtaaaac ggtttccgat ttttcgccga gaaatgattc gcagaaaatt ggcacaagat
      901 gtttctgaag aagaaatcgt ccatttgatc cataacatgg aacaaatgtt gcatcttagt
      961 tattctgatg agtcggtaga ctatttgcag acatcagcga gatttttagt aagccgtggg
     1021 atcaagatgt cagataagca cgcgtttttt cccgatgatg tgacggatac tcctgaattc
     1081 cgaatgctga tgatcttggt acatgaaaca ggttggagtc tatccaaaag ttatatggag
     1141 tggttcacgt tattattttt aacttccaat atttttgaga gcaaaactac tcaaaattat
     1201 gattcggatc aagaattgcg aaaattgatc agccaaatgg ttgaaaattt ccaaaatcaa
     1261 acgttgattt tgatcgatga ccgagaagac tttgaacgta ggatactgag tcatctgcgt
     1321 ccagcctgtt ttcgaatacg ctataacttg agtttaggag tttattcaat agatagtctg
     1381 atcaaggata gtaatcatgc gattttgatt gatttgatga aagaattgat tattcctatt
     1441 gagaattggc taaataaggc ctttccaaat gatgaattag atcttttatc ctattacttt
     1501 ggctatcaat taagtaatca taataaattg aataagcaac gacaaagggc tgtagttgtt
     1561 tgtactaatg gtgtcatggt ctcaaagtta gtacgagcca atatggaaaa actttttcca
     1621 gagatccatt ttttagcttc cctatctgtg cgtgatttct acaaatttga agctgattat
     1681 gatctggtct ttactacgac gccactaaaa agttctatgc tgcagtttat gatcgagcca
     1741 atcatgacat atcagcaaca aatcagttta cgatatcgag ttttgagtga tttaggaatt
     1801 actaaggttg atcgagcaat ggatgaattg ttaacaatca ttaggaaata tgcaaatatc
     1861 accaatccag tttcgttgcg agaagaactg cagtactttt tgatcaagga agatggaagc
     1921 acgccgctgg acaacttcaa tgtattgcct tcattgactt attacttgcg gccgagttat
     1981 gtacaaatcg ttgaagaaga gatgtcttgg gaacgagcag tggaggtggc ttgccgaccg
     2041 ttattggaac atcaaatcat tgactcgcgt tttatcgctg attgcttgga tcaaatcaga
     2101 caaccgggat atgcaggtta cttgggaatg aagacttgca ttccccatac aacggtcgat
     2161 aaaggtgtaa tcagtgacgg tgtcagcatt ttagtttgta aaaagccgat acgatttcct
     2221 aatggtgaag atgtcagctt gattttgccg ctttcatttt ttgatttaac gaagcatctg
     2281 cgagccatca atcaaatcgc tgatatcgcc aaagatgatt cattgattga gcagttattg
     2341 attgctgatg acgaaaagac agtttatcaa ctattaagac aaagatctta aggaggaaaa
     2401 catgagacta gcagattatc ctaaattgac agtgatcatg cgtggttata cttttgatca
     2461 ggcagatgct attttacaag caatgcagga atttgaccat caatttgccg tagagatgac
     2521 aatgaacaca aaaggagctc ttgaaaatat acatgaacta aatcaacgtt atggtgacac
     2581 gacttatatc ggcgctggga cggtaaggac attagaacaa gcccaagcct gctatgaggc
     2641 tggtgcaaaa tttttattag gaccacatat gttcacaaaa gaaatgttgt cttacgccaa
     2701 taagaaagga ttattggcgg tacctgccgc aatgactcct tcagaagtcg accgtatgtt
     2761 taaagaagga gctgatattg taaaagtatt tcctgcagcg gttgttactc cacgtttttt
     2821 caaggatatt caagcacctt taggtaagtt accactaatg ggggttggtg gaatcagtaa
     2881 ggagaatgcg aaagagtttt ttgaacaagg tgctagctat ttaggtcttg gttccggtat
     2941 gttcaacaag caagacatcg aagagctaaa tgttaaaaac ttagcacgtt cgatgaaaga
     3001 attaattgag gctattaaat aggagtgagg actttggaac tttatttgaa tgaggaactc
     3061 gtttttcgaa atgttcaagc aaaaacagat agtgaagtct tagcgttttt agcaagcgag
     3121 atgtacaaaa aaaactacgt gaaggaggaa tatattcaag caatccaaga acgggaaaaa
     3181 gaatacccta ctggattacc atcaacacca ccaggaatcg caattcccca tgcaaactat
     3241 gaaatggtca ataaaacaac tttagcaatt gcgactctta aagatccggt cctatttcat
     3301 aatatggaag ataacaatga gcagttacca atccaaattg tgatcatgat ggcgattggg
     3361 gaaccacatg ggcaagttga aatgttacaa aaaattgttg gaatcataca agatgagcca
     3421 ctgcggcaag aaatgatcag agcaggcaat gatactgaat tattagaact attaaagaaa
     3481 gcggtttttt aatataagga ggaaatcata atgaaacgaa ttttagtagc atgcggaaat
     3541 ggtatcgcca cttcaacagt agtagcaaca aaagttagag agtatttgca acaacatggc
     3601 attgatgttt caacgactca aacaaaattg atggaagttc caggaaaagt tcaagactat
     3661 gatctattgg tgacgactgg tcaatttgat ggtcaaacag gtggtgtacc agtaatcaaa
     3721 gggatgccga ttttaacagg tatcggtgcc gatcagacaa tggaagagat tttgaactta
     3781 ctgaaataat agctgatcaa aggagctgaa aaaatgaaaa tcattatgaa cgtctttaac
     3841 ttctttattg atgctggacc aacagttatg ttgccagtca tcatcacgat tattggtttg
     3901 atttttgggt taaagatttc tcgtgctttt aaatcaggtt tgacattagg tatcggtttt
     3961 gccgggatca agttgatatt ggactttatg acgaccaatg ttggaccagc tgccaaggcg
     4021 atggttgatc gaactggtgt taaactagat gctttggatg ttggctgggg ttcgattgcg
     4081 gctgttactt gggcatcgcc aatcattcca atcttgattt ttgccattct attggtcaat
     4141 atcgttttgc tgattttgaa acgtacacat acgctggatg ttgatatctg gaactatcac
     4201 catatggcaa tcgttggtgt tatagtgtat tttgtgacta agaatgtctt cttaggtgta
     4261 ggtgcatcag tagtgatggc aattgcgact tttaagattt cagactggtc gcaaccgatg
     4321 gtagaaagtt tcttcggaat ccctggtgtt tctttaccaa ctgtttctgc gctttcatca
     4381 ctggttattg cttggccgtt aaattggctg ctggatcgta tcccgttatt cagaaaatca
     4441 aaattcacca tcaaagatgc acaaaaatat ttgggatttt ttggtgattc tatgatcatg
     4501 gggttagtta ttgggatggt gattggtgtt ttagccggct atgatatcaa agcggtttta
     4561 caattaggtg tcagcatgtc agctgttttg gttttgattc ctaagatgac ttcattattt
     4621 atggaaggat tgatgccaat ttcagatgct gcacaaaaat ggtcacaaaa aaaattcaaa
     4681 ggtacacgtt tatttatcgg attggatgct gcagttgttg taggtaatcc agatgttatc
     4741 acaacagcat tgatcattat tccactaacg attgcattag ctttagtatt gccaggaaac
     4801 cgtgtgttac cattcgcaga tttagcggtt gttcctttcc gcgtggcaat ggtcgttgcc
     4861 ttgactcgtg gtaatttatt aaaaaatatc attattggct tagttgtgac agcttcattg
     4921 ctatggtgtg ggtcagctac tgcgccaatg ctgacagcaa ttgccaaatc ggttggtatt
     4981 aacttaggaa cttcttcaat gttaatctca tcatttgctg cgacttccat gatccaaagt
     5041 tatttggtat ttatcgcatt tgcttacaaa ccaatcatcg gtattccagt gttgttatta
     5101 gcctttgcgg gcgcatggta ttacttcgat ggtatcaaaa aagttaatca tgaaatggat
     5161 gaaccattga aggcacaata gtaggagggt ttaaattgaa aatttcagat gtcacagtgt
     5221 ataaagtaaa accaagatgg atttttgtta agatctcgac tgacgaagga atcgaaggct
     5281 ggggagaaat gatctcagga actaaaacag aaacggttgt tgctggagct tatgagatag
     5341 gaaaccggct gattggacgt aatccgtttg aaatcgagcg cctcttccaa gaaatgcatc
     5401 gatctttctt ccgaggcggt cctattaatg gaaccatcgt ttcgggttta gaaatggctc
     5461 tgtgggatat caaaggaaaa gcgtttaatg ttccggttta tgaattattg ggcggtgcag
     5521 cacgtgataa aatcaaggtt tattcttgga ttggtggaga tcgaccaagt gaagttgcag
     5581 aacaagcaca agatcggttt gatcggggct ttacggccgt caagatgaac gcgacagaag
     5641 agctgcatta tatcgattcc taccaaaaag ttgatgaagt ggtggaacga gttgcgtcta
     5701 ttcgcgaacg ttttggtgat gcaatggata ttggtgttga ttttcatgga cgggtccata
     5761 aaccgatggc aaaagtgcta gcaaaagctt tggagccatt ccatccaatg tttttggaag
     5821 aagtcgtttt acctgaaaat gaagaacact ttaaagaagt agctgattct gtggctgtac
     5881 cattagctac tggtgaacgc ttgtacactc gttggcaatt caagaacctc tttaaacaag
     5941 gtactgttga tattatccaa ccagatgtgg ctctttgtgg gggaatcttg gaaacccgta
     6001 aaatcgcagc gatggcggaa gcttacgaca tggctgttgc gcctcatgcg ccatatggtc
     6061 ctgttgcact agcagcaact ctgcaagtag actcatgtac gccaaacgtc tttatccaag
     6121 aacaaagctt agggatccac tataacaaag gttttgatct gttagatttt gtgaagaaca
     6181 aagaagtttt ccaatataaa gacggctacg tagatctacc ttcaaaacca ggtctgggct
     6241 tggaaatgga tgaagacaaa atcaaagaaa ttgcacaaga aggtttgatt tggaccaatc
     6301 cgcaatggaa aaattatgac ggtacgatcg ctgagtggtg ataaatgaaa ataaaggatg
     6361 atgaaagatg gcagaagcac ttatgatcac agttttttta ggactgctga ttattttgca
     6421 aataatcgat caaaagaaaa ttatcgtgtt tgctcagagt agtcctgtaa ggaagagttt
     6481 gagtatttta ctgacagtcg ccatcctgtt gattttctgg ccggaacatc tgactgatca
     6541 gattaaactg gttgcttttg cttctgtcat tttgatcaat ggttttttga aggagggtct
     6601 agcaaaagat tctgttgtta gatttggagt attgaacggt aactttaatc gatatcctca
     6661 agtccaagta gaaaaactag gtgacgataa atcatttatt tcattttaca aaagcaagaa
     6721 taattctttt tcaatgattt ttgataaaga tcaaactgaa atgatggatg tctttcaaga
     6781 acttgatatc cattccaaaa ttactttaga tcaaagttcg gaagaaaaaa gagcagttgt
     6841 aaaaaaagat aatgaatgat tctactaccg aaattatgat ttctttatca cgaataatga
     6901 aacttgtttt aatgaatcat attgattgaa gtatacaatg tatattaagt tttggaaaga
     6961 aatatattgg tacaagatat agcaggaatc gattactcga tccctgcttt ttttaacttc
     7021 ctttgcagca aaaaaagctc gtaatctaag agatgatgga tagtttttag aatatatata
     7081 ttttcttcaa ttccagaaaa aaatgaccaa gctagtatgc taaaaaagta cgatgcttct
     7141 atgatctttt ttccgtcttt attaacagat gttttaattg atcaacgcat attgtaatct
     7201 accctaattt ccatagttct tcaccacttt taaaaatagt aaaaccccta caagtatgct
     7261 atagtaaatt gctaaagaaa aaccaataag gtgggagatg tgtgcgcgat caaacaagcg
     7321 gaaaaatgat tcgtttttat tatacaagtt tgcattttct ttattggatg atgttttgtt
     7381 ctgtctatgg gtttgcgaca ttgtttttga tttatttcaa gattgatcca gctaaaatcg
     7441 gagggatctt agcattggtc aatattgttt caacggtgct tcagcctttt gtcagtcaag
     7501 tgatcatcaa gaaaagaaag atcccactga tcaccgtgct gaagacgatg atagcaggat
     7561 taggagtgat tctcttagct ggggtattga ttccgaattt gccgatcatt acttatatct
     7621 tgggtgggat tttagtagtt tctatgcaat catttctcaa tgcgctggca tttgagtatt
     7681 caaataccgg ctacaggatc aactttggct tttcaagagc tggtggttca ttttcttatg
     7741 ctgtaacttc ttttttactg ggacagctgt tagcgattta ttctgccgcg attttgccaa
     7801 tcttggttat agttgaagca gtgatatttt tcgttatttt gcatctgctg ccggatgtaa
     7861 aaaatcaaga agtttttcct atgacaaaag aacaggatcg ctcagtttct ttgagaaaaa
     7921 aatatccttt cctgattttg ttatttttag gtttcagttt tctttttact ttccacacca
     7981 tgatcaacag ctttttggca cagatctttg aaaatatcca cggcggcagt aaagaagttg
     8041 gcatcgcatt gatgatcgcc gctttatgcg aattaccggg aatgatcttt tttgaacagc
     8101 ttgtcaaaaa gaaatccagc aaattttggc tgtctgttgc cagtatttca tttgctgtca
     8161 gaggattcat gatgctgctg gccagttcga tcctgatgat ggaaatgacg catctcctac
     8221 aagccttcag tttcgctttg tttattccag cttcggctta tttgttcaat caatttctgg
     8281 cggatgatga tcgtgtattt ggacaaacga tgatcatcat cgccacaact cttggtgggg
     8341 tgataggaaa tgtcttgggc ggcagtctgc tgcagcattt cggtgtatgg gagatgctga
     8401 ttttcggtac tggttttgcg gtgctaggtg ctttattaac tattttgggc attcgaaaaa
     8461 taccgaagac aggaaacgat agtgattctc ttgttgagta gcataaaaat acttagatac
     8521 tagttttata taaataacat atagtgatta ttggcgtaat tacaaaaaac gagaataact
     8581 taagtaagca tttctaaata attgagaaac ctcatctaaa aagataagaa aatatcccta
     8641 ataaaacttc tgtaacattt tagataactg attgtaatct gaataataaa ctataaggag
     8701 acggttagac ggtctccttt ttttgcgtat tataattcta gaattgttgg aaattgataa
     8761 taattaacaa gaaatattag acaggaactt cgaactgctt atcacattct ta
//
LOCUS       DACCDF010000097         8555 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCDF010000097 DACCDF010000000
VERSION     DACCDF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.101"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK51_002425"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IVK51_002425"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP6431981.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IVK51_002426"
     CDS             complement(1098..2201)
                     /locus_tag="IVK51_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6431982.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IVK51_002427"
     CDS             complement(2291..3226)
                     /locus_tag="IVK51_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP6431983.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IVK51_002428"
     CDS             3528..5462
                     /locus_tag="IVK51_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6431984.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IVK51_002429"
     CDS             5919..6335
                     /locus_tag="IVK51_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP6431985.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IVK51_002430"
     CDS             6349..6915
                     /locus_tag="IVK51_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP6431986.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IVK51_002431"
     CDS             6938..7924
                     /locus_tag="IVK51_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP6431987.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IVK51_002432"
     CDS             complement(8079..>8555)
                     /gene="scm"
                     /locus_tag="IVK51_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6431988.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2517 a         1650 c         1737 g         2651 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgct
//
LOCUS       DACCDF010000098         8461 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCDF010000098 DACCDF010000000
VERSION     DACCDF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.102"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(487..801)
                     /locus_tag="IVK51_002433"
     CDS             complement(487..801)
                     /locus_tag="IVK51_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP6431989.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(815..1075)
                     /locus_tag="IVK51_002434"
     CDS             complement(815..1075)
                     /locus_tag="IVK51_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP6431990.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            1245..1559
                     /locus_tag="IVK51_002435"
     CDS             1245..1559
                     /locus_tag="IVK51_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP6431991.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1561..3216
                     /locus_tag="IVK51_002436"
     CDS             1561..3216
                     /locus_tag="IVK51_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6431992.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            3299..5434
                     /locus_tag="IVK51_002437"
     CDS             3299..5434
                     /locus_tag="IVK51_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6431993.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5609..6118
                     /locus_tag="IVK51_002438"
     CDS             5609..6118
                     /locus_tag="IVK51_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6431994.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            6115..7419
                     /locus_tag="IVK51_002439"
     CDS             6115..7419
                     /locus_tag="IVK51_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP6431995.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFI
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEEISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            7515..8384
                     /locus_tag="IVK51_002440"
     CDS             7515..8384
                     /locus_tag="IVK51_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6431996.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
BASE COUNT         2869 a         1476 c         1733 g         2383 t
ORIGIN      
        1 aaaaaagggg tgtgacctat gtcgcattcc tttttttgat aaatagactc ccagtagcta
       61 cttctcgata ataagccaac caaaccaaaa acaggagaag ttgtttttga aattatttct
      121 tgtgattcgg agttaaatgg taggctcaca acctcttggt aaacggtgtt caatcagctg
      181 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctattttccc gtttttttct
      241 ctaatactta ggtcaaaccg ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct
      301 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt ttgtttggct
      361 gttcttatta cttgatgcag gacagcgttt tcacaacctt tgattcacgg ttttccagaa
      421 gtagcttctt cgaaaaataa gccgaaattt cttgaagctg atcacttctg tcgcaacctc
      481 tactgactaa ggcttgatat aagcataacc ttgggattgc agttcaacta aatcaaggac
      541 acctgctggt acaacgatta cttgttcagg taaagaactt tctgaaatat gattagcacg
      601 catagcattg gcgcaagcgt gaaaaacgac tccttgcaaa tctaagaatt ctgcattggc
      661 agagtctaga taaccggtaa tcgcgatgcc atttacagat acgacaatcg tcgtttctgg
      721 agaagctttt atcaggtttt ttacgttttt ccctgtttct gaccattttt ctaattcatc
      781 aatatggaaa acgactttca ttgaaatcat tcctttactt tttaataatc atcttgatcg
      841 cttgttctag tttagccgct tgttcttctg gattactatc aggtttctca aagcaatttt
      901 tcagattatc tgcaacgatc atgcccatta cgcgatcaat actagaacga acagcgctta
      961 attgagtgat gacatccata cattcttttt cgtcttcgat cattttttgg ataccacgaa
     1021 tttgtccctc tgtacgtttc aaacggttca ggatattttt attatcaatc tccataacac
     1081 attctcctct atttttataa ttagataata ccccttatcg gtattttatg caaatataaa
     1141 aagtagtgaa aaaaatgaag agtcattctg gttgacatat taataaagct cctgtttaat
     1201 ataccccatg aggtatttat aaaaatgtaa atgaggtgca gaaaatgaat ttgctatttc
     1261 aatcaataaa atcgataaca gtcccagaat taaaggaaaa gcttttggaa aatccagcgt
     1321 tacttgatgt tagaacacct gcagaatacc ggggcgggca tataaaagga gctaaaaatg
     1381 ttccattaca aagtataaac agatatgatg gagacaaaga aaagaccgtc tatgtgattt
     1441 gtcaatcagg gatgaggagt aaacaagcag ctaaagaatt aaagaaatct ggttatgatg
     1501 tagtgaatgt acgtggtggc atgaatcaat ggtttgatcg aacagtagga gggaaataag
     1561 atgaaaatcg ttattgtcgg aggtgtagca ggtggaatgt ctgctgctac acggcttcgc
     1621 cgattaatgg aagatgcaga aatcgttgtt tttgaaaaag gaccgtatgt ttcttttgca
     1681 aattgcggct tgccttatta tctttcggga gaaatcagtg aacgggaaaa tcttcttgtc
     1741 caaacgccag aatcattatc cgctcgtttt tgtttagatg tgcgtccaaa tcatgaagtg
     1801 acagccatct ttcccgaaaa caaaacggta gaagtcgtac atgagggtca aaaacacatt
     1861 gaacagtacg atgcattggt tttatctcct ggtgcaaaac cagttgttcc atcgattcca
     1921 gggataacag aagccgacaa tgttttttct attagaaatg taccagatat cgataaagtg
     1981 atgcatgcat tagaaaaaca gccaaagcgt gccgtgatcg ttggtgcagg attcatcgga
     2041 ttggaaatgg cagaaaacct aaaaagaaga ggtttagaag tcatggtgat cgaacaagca
     2101 ccacatattc ttccaacact ggatgaagaa atggcagctt ttatagaaaa agaattgtct
     2161 catcaaggag tagaagtgat tacttctcat gctgtcgctg gatttgaaga ccacgggaaa
     2221 cgattgcgac tggatgatgg gcgtaccatc cctgctgatt tagttatttt atccattggt
     2281 gttcgtcctg ataaccagct agcagtgacc gctggaatcg aattaggcat acgcgggggt
     2341 atcctagtag acgaacgtta tcaaacgaat attcctgata tttatgcggt gggggatgct
     2401 atcgttgtaa aacagcaaat cactggaaaa gatgcactta tttctcttgc ttcaccagcc
     2461 aatcgtcaag gtagacaagt tgcggacacg atttccggaa tttctcgaag aaatcaaggc
     2521 ggtattggaa cagcaattat acgaacgttt ggaatgactg ccgcatccac cggtttaagt
     2581 gaaagaacag ccaaagaaaa cgaactgtct tttgaagtca ttcatgtatc aggaaaagat
     2641 catgcaagct attatccaga agcaacagat attttactga agttgatctt ccatccagag
     2701 actggcgaga tttatggtgc acaaggtgtt ggggcaaaag gtgtggataa acggatcgat
     2761 attttagcaa cagcaatcaa agggcatttg acgatcttcg atttaccgga attagaattg
     2821 acgtatgcac cgccatttgg ctcagccaaa gatccagtaa acatgctagg atatgcagca
     2881 atgaacattg cagaagggct tagtgaaacc gtacaatggc atgaattgcc gacagaacta
     2941 gcaaaaggaa aaattttatt agatgtgcga acagcagaag aattggaaaa gggcaaattc
     3001 aaggaagcca aacatatccc tttgaatgaa cttcgggacc gattaaatga attagacagc
     3061 caacaagaat atatcgtcag ctgtcatagc ggattacgta gctatctagc ggaaagaatc
     3121 ttgaagcagt ctggctacca cgtaaaaaac cttgatggtg cattttcttt atatcaaact
     3181 gtccgacctg aagaactgat atatcctaac aaatgatact cgttaaaaag cttgcattcg
     3241 actcttaaaa gaggtaaact aatcggttcc accactttaa aaaaagagga gaatgtgtat
     3301 ggtcaacaca caaaaacagt tagaagaagc atatctgaag aacgtgtatc aaaagctaat
     3361 tgataaaaaa aacgaactgc gggaatcgat ggatacggca aaaatagaag gaaaccagtc
     3421 gttgaaagaa atggctggcg agcttcgatt gaattttgac agttacttag ataacctgga
     3481 tactttttcg atgattgaaa tgaaaaatag agaaatcgat cagatgaata tcaaacagaa
     3541 aaacgcagaa aatcagctag caaagatcga acgtttattg aaaagtccct actttggaaa
     3601 agtcgttgta gactttctgg ataatgagcc aaaagaatcc ttttatatcg gtacagcaaa
     3661 tttcacagat gatgcagatg aaaatcttgt ttatgattgg cgttcaccaa ttgccgaact
     3721 tttttacaat aacatgattg gtccatcttc ttatacggtt cgtcaaaata aaatcgatac
     3781 ggttattaaa gaaagacgac aatttctttt ggaaagagat cgtttggtca agttttttga
     3841 tacgtctgta gctattcaag atgacgtatt attagaagtc ttaggccaag atgagaccaa
     3901 tgaaatgaag gctatcactg ctacgatcca atcggaacaa aacacaatca ttcgggatgt
     3961 gaaaagtcaa aatatcttgg taaatggcgt agcaggaagt ggtaaaacct ctgctatcat
     4021 gcagcgtatt gcgtatctgc tctatttata tcgagatacg atgacatccg atgacttgct
     4081 gattctatca cctaatcatc ggtttatcga ctatatttcg aatgtcctac catcattagg
     4141 tgaaagaaat ccactgaatc taaccatcat acagctggtt tcacagcttt cagcagagga
     4201 aatagaaggt gaagaggctt atttcaaacg tatcacggga gaaaatgtat cggagcagac
     4261 agaacgtttg cgtagcaaaa agtttatcga taatctaaaa caatcagatc ctttgttttt
     4321 agatcatccg aattttatcc gtggacttac taaaaatgga aaaacagtcc tttctaaaaa
     4381 aacgatcgaa aaaatatatg agaaagttcc tgctcatcct aaattgatcg ataggcttca
     4441 agcaacgaaa aaggcactga tgagcgaatg gaagaatcat cttttgaaac aagcaaaaag
     4501 tcctgctgtg cagaatcagg tactctcgtt gacagaagat aggcagttgg aattattcgg
     4561 taaattgatt tctgatgatt cggaacaaag tattgtagct tatgcaagga aacttttaca
     4621 aaaaaaatat cgaaaaataa ccagacaaat cgaagaaatg gcatgggtgg atgagcatca
     4681 gttgtttgaa agaatttatg aaaaacgata tggctcagct tatgcttggc aacccacacg
     4741 tactgttgat gaagcagtca ttatattagc tattcgtcat ttacttgttg aaaaggtaaa
     4801 tgtaccagca tttcgctatc tattgattga tgaagtacaa gattataccc tggcacaatt
     4861 aggtctattg atcgaattat ttccaaaaac tcactttact ttagtaggtg acgagaatca
     4921 ggcaatcttt aattcttcca ctacttttgc tgatattatg cgttgtttcg acgactacca
     4981 tctgccgatc catcggtatg atcttagaaa tagttaccgt tctagcggag caatcacaga
     5041 gcttttcaaa acctatgctg tggatcaaga gaagatcgac atcgtatcga tccgaccgca
     5101 aggaaaagaa cccgaatacc gctcttttcg atcctctgaa gaattgttga aaatactagc
     5161 tgagattttg gtttctctaa aaggagaaaa agcggcagtt atcacccaaa cagaagagga
     5221 aacccaaacg ctacaagaat cgattaaaaa aaattcatat ggattagaaa actgccaaat
     5281 cattcctttg agtctagcaa aaggactgga atttgatcat gtaatcttat atccatttga
     5341 aaacgatgga gatgagcaaa gaagacgcag acaaatgtac acggcgatat ctagaggaat
     5401 gaaaagcatt gtcgtattgg aaagagcaac ataattaaga gataaaaaat aaaaagaaca
     5461 actgataaaa agaacaaaat atttttcatt tgctgatgag aaatgttttg ttctttttct
     5521 taggaaaaat ttaggaacac tttatgtgaa aagaaatgaa atgtatggta aactcctacg
     5581 tcgtaacaaa aaaacgtgag gtgaggatat gaaaaaagta acaagagaag aagttgcatc
     5641 aattttggtg aaagataagt atttttcaaa aggtaattac tggttaaaga tttggcaaac
     5701 gttagtggct cttttaggat ggatgatcgt tgttcttcct tttatatggg tctttttccc
     5761 attaacgaga ccagaaagag ccaaggattt ttccttgtac gcttttttat cagaaaagaa
     5821 aatgctgtat ttcttaatcg gcttttttgt attgattttt ttcagtttcc tgattacctc
     5881 atttgtattg actaggtgga acaatcgaaa tctatacaaa aaagtaaata aatcttttga
     5941 atatgaagat gaaaaattga aaaaaagaca agaattactt gaagaagtgt atacggaacg
     6001 ttttggaaca aaagaagaga gagaaacggt ccgtttttat tctgtctccg aagagaaaaa
     6061 tctagatacg acattcattg cagatttgta taaagaaaat ggggtggaac tgaaatgatc
     6121 tcaatcaccc atacaggtaa tttattctta gatacttgtt tgtctattat gtattttttt
     6181 ctcgtttctt atccgatttt aggcggattt gtatggttta ttggtgtctg gtgttatgtg
     6241 tttatataca aacataagca aaaagaatgg gtagatgttc ccttgagtgt cgaaccgttc
     6301 atcacgatca tggtgccagc ccataatgaa gaaattgtga tcgaatatac aatcgagtat
     6361 ttgatgacga aattgaatta tcacaactat gaggtattag tgacagacga tggttcaacg
     6421 gaccaaacac cagaaatatt agctagactg atgaaaaaat acgctaatct tcgtgtggtt
     6481 cggatcgaaa aaaataaagg aaaagcccac gcgtttaata tcggacttgc ctttgcaaaa
     6541 gggaaattga ttttaagcaa tgatgcagac acggtaccgg agccagatgc actgattagg
     6601 tatgtgaatt atttcattcg ccctggtgca agacatattg ctgcggtcac tgcaaatatg
     6661 gatgtccaaa atcgtaccaa attgattgca aaatctcaaa cggttgaatt ctcaagtatc
     6721 gtgggcatca ttaaacgaac acagtcagct gttttcggtg gtctttatgc gtacagtgga
     6781 gcaaatacga tgtatcgaaa agaagccttg atcgatgtgg gcggattcag acaagatcga
     6841 gcgacagaag aaatcagtat tgcttgggat catcagttaa atgactggtt atctgttttt
     6901 gctcctgaga ttatcttttt catggaagtt cctgtgacgt tgaagatgct atatcgacac
     6961 agaaaacgtt gggcaaaagg tggaacagaa gtctggctga cgaatttcaa aaaagtgatg
     7021 ctccatcctt ttaaacatat tggaagaacg atcatcttca ttgatcaaac attgagtatt
     7081 gtctggtcaa tattcttttg tatttccgta gttctttttg ctggattaat cgggcattat
     7141 gtatatcaag gaaactacga acagatttat attactttta cattcagttt tgtgttcatc
     7201 tgctttgaaa tggtcgcagg atttttccag ttgctagctt cgctgattgt agatgatcga
     7261 cgaagaaaat tgaaatacct tcttttcgca ccactatata tgcttttgtt ctggatagtg
     7321 aacgcgataa cgatcgtgac gactttcatt ccagcagtca aaacgatttt aggttatgga
     7381 agcgggacat ggaaaagccc ggaacgtaca aaaaaataaa agatgcaccg ttttttagac
     7441 aaacagcctg ttgtgtttat ggttcttcca aacatttttg cttataataa gaaattagtt
     7501 aaaaagtagt aaggatggga aaagtgatga aaaaaagaat gaagccgtta atctggccaa
     7561 ttgctgtttc cagtattttc ttattgggac agacagcttt tgcggaaact tctgaacctg
     7621 ctcaaacaga accttcaaca accataagct cttctggtgc tgaaaacggt gcctcggaaa
     7681 cagaaatgac ttccaatgta ccgacagata attcaggaga aacgatttca tcttcagaag
     7741 atacatcaat aaatagttct acctcttctg accaaatcga tcatttgcaa gacttggaaa
     7801 ataaaagaaa agaactcgaa gaagcattag aagctgcaaa aaacaaattg aaagaagacc
     7861 aagcagcaat cgatcaagat ttagctgatc ttaaaaataa agtgggaaat gaccgtgcac
     7921 aacttgagaa aaagcttgcc gaagcgaaag caaaagcgga agctgtccaa acagatcttg
     7981 acgggaagat ccaacaagca aaagaattca atcagcaatt aaatcaggtt gctcaaacag
     8041 cttctggaaa gctaagtcaa actcaagcgg atatcacagc ggccagtcaa gcaatccacg
     8101 gaaagatcag cgaagcacaa acagttgcta atgattttga agcaaaaaaa gctgacttag
     8161 cagctagttt ccaacgaata aaagatattt tgcattcggc aggagaaaaa tcagtgacct
     8221 ctggaagcac gacagaaaca gttcagcaaa cgacagagcc atccaaatca acaagtcaga
     8281 ctttgccaaa aacaaatgaa aaaacagctt ctgtcagtta ttccttagca ggggcgggtg
     8341 tgttagtcgc agcagcttac ggaatcatcc gcaaaaagaa ataaaaaaga gaggatggga
     8401 aggtagagta cctcgaagtt agactgaaaa atctaattct tgagtgccac tacatgtccc
     8461 a
//
LOCUS       DACCDF010000099         8425 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCDF010000099 DACCDF010000000
VERSION     DACCDF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.103"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            471..2108
                     /locus_tag="IVK51_002441"
     CDS             471..2108
                     /locus_tag="IVK51_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP6431997.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IVK51_002442"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IVK51_002442"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP6431998.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IVK51_002443"
     CDS             complement(3184..3819)
                     /locus_tag="IVK51_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP6431999.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IVK51_002444"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IVK51_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP6432000.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IVK51_002445"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IVK51_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8425
                     /locus_tag="IVK51_002446"
     CDS             6417..>8425
                     /locus_tag="IVK51_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP6432001.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2800 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaat
//
LOCUS       DACCDF010000100         8290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCDF010000100 DACCDF010000000
VERSION     DACCDF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.104"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..1511)
                     /locus_tag="IVK51_002447"
     CDS             complement(45..1511)
                     /locus_tag="IVK51_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP6432002.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IVK51_002448"
     CDS             1898..2494
                     /locus_tag="IVK51_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6432003.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSDGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IVK51_002449"
     CDS             complement(2552..3034)
                     /locus_tag="IVK51_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432004.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IVK51_002450"
     CDS             complement(3140..3337)
                     /locus_tag="IVK51_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432005.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IVK51_002451"
     CDS             3632..4390
                     /locus_tag="IVK51_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP6432006.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IVK51_002452"
     CDS             4450..4878
                     /locus_tag="IVK51_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432007.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IVK51_002453"
     tRNA            4924..4996
                     /locus_tag="IVK51_002453"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IVK51_002454"
     CDS             complement(5146..5517)
                     /locus_tag="IVK51_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6432008.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IVK51_002455"
     CDS             5732..5917
                     /locus_tag="IVK51_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432009.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IVK51_002456"
     CDS             complement(6000..6188)
                     /locus_tag="IVK51_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432010.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IVK51_002457"
     CDS             complement(6212..6475)
                     /locus_tag="IVK51_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432011.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IVK51_002458"
     CDS             6633..6890
                     /locus_tag="IVK51_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP6432012.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IVK51_002459"
     CDS             complement(6961..7152)
                     /locus_tag="IVK51_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432013.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IVK51_002460"
     CDS             complement(7293..7613)
                     /locus_tag="IVK51_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432014.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IVK51_002461"
     CDS             complement(7636..8070)
                     /locus_tag="IVK51_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP6432015.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2627 a         1635 c         1315 g         2713 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat cagacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg
//
LOCUS       DACCDF010000101         8284 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCDF010000101 DACCDF010000000
VERSION     DACCDF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.105"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            213..1406
                     /locus_tag="IVK51_002462"
     CDS             213..1406
                     /locus_tag="IVK51_002462"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP6432016.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1536..3008
                     /locus_tag="IVK51_002463"
     CDS             1536..3008
                     /locus_tag="IVK51_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6432017.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3091..3879
                     /locus_tag="IVK51_002464"
     CDS             3091..3879
                     /locus_tag="IVK51_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP6432018.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3946..4614)
                     /locus_tag="IVK51_002465"
     CDS             complement(3946..4614)
                     /locus_tag="IVK51_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP6432019.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4729..6441
                     /locus_tag="IVK51_002466"
     CDS             4729..6441
                     /locus_tag="IVK51_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6432020.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6444..8216
                     /locus_tag="IVK51_002467"
     CDS             6444..8216
                     /locus_tag="IVK51_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6432021.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2537 a         1387 c         1821 g         2539 t
ORIGIN      
        1 aaaagcttag cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa
       61 tgtaaaatat ttttatattt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
      121 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
      181 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
      241 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
      301 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
      361 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
      421 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
      481 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
      541 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
      601 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
      661 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
      721 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
      781 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
      841 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
      901 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
      961 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     1021 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     1081 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     1141 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     1201 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     1261 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     1321 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     1381 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     1441 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     1501 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     1561 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     1621 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     1681 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     1741 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     1801 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     1861 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     1921 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     1981 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     2041 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     2101 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     2161 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     2221 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     2281 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     2341 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     2401 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     2461 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     2521 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     2581 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     2641 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     2701 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     2761 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     2821 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     2881 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     2941 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     3001 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     3061 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     3121 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     3181 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     3241 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     3301 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     3361 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     3421 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     3481 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     3541 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     3601 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     3661 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     3721 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     3781 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     3841 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     3901 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     3961 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     4021 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     4081 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     4141 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     4201 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     4261 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     4321 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     4381 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     4441 agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     4501 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     4561 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     4621 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     4681 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     4741 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     4801 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     4861 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     4921 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     4981 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     5041 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     5101 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     5161 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     5221 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     5281 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     5341 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     5401 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     5461 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     5521 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     5581 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     5641 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     5701 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     5761 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     5821 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     5881 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     5941 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     6001 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     6061 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     6121 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     6181 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     6241 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     6301 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     6361 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     6421 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     6481 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     6541 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     6601 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     6661 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     6721 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     6781 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     6841 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     6901 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     6961 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     7021 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     7081 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     7141 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     7201 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     7261 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     7321 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     7381 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     7441 atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     7501 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     7561 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     7621 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     7681 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     7741 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     7801 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     7861 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     7921 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     7981 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     8041 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     8101 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat gagttattag
     8161 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa agttaaatta
     8221 aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt ttttataagg
     8281 ggca
//
LOCUS       DACCDF010000102         7623 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCDF010000102 DACCDF010000000
VERSION     DACCDF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.107"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(14..706)
                     /locus_tag="IVK51_002468"
     CDS             complement(14..706)
                     /locus_tag="IVK51_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432022.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVK51_002469"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVK51_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVK51_002470"
     CDS             complement(1143..1712)
                     /locus_tag="IVK51_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6432023.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IVK51_002471"
     CDS             complement(1786..2109)
                     /locus_tag="IVK51_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432024.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IVK51_002472"
     CDS             complement(2263..3075)
                     /locus_tag="IVK51_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432025.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IVK51_002473"
     CDS             3429..4277
                     /locus_tag="IVK51_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP6432026.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IVK51_002474"
     CDS             4422..5363
                     /locus_tag="IVK51_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP6432027.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IVK51_002475"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IVK51_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1511 c         1371 g         2469 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt aggatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcggcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggttcg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaaa
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag ttacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgagaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgaa tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tcgaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aac
//
LOCUS       DACCDF010000103         7596 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCDF010000103 DACCDF010000000
VERSION     DACCDF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.108"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(135..425)
                     /locus_tag="IVK51_002476"
     CDS             complement(135..425)
                     /locus_tag="IVK51_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432028.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(543..803)
                     /locus_tag="IVK51_002477"
     CDS             complement(543..803)
                     /locus_tag="IVK51_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432029.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(940..1353)
                     /locus_tag="IVK51_002478"
     CDS             complement(940..1353)
                     /locus_tag="IVK51_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP6432030.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="IVK51_002479"
     CDS             complement(1809..2294)
                     /locus_tag="IVK51_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432031.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2678)
                     /locus_tag="IVK51_002480"
     CDS             complement(2433..2678)
                     /locus_tag="IVK51_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP6432032.1"
                     /translation="MFYHLRKENSNENLDAIALTLLIVGGLNWLLVGLFELDLVAMIA
                     GGSTTIFAKIIYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IVK51_002481"
     CDS             2871..3671
                     /locus_tag="IVK51_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6432033.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IVK51_002482"
     CDS             3690..5558
                     /locus_tag="IVK51_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6432034.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRSYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IVK51_002483"
     CDS             5551..6042
                     /locus_tag="IVK51_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP6432035.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IVK51_002484"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IVK51_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP6432036.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IVK51_002485"
     CDS             6602..>7596
                     /locus_tag="IVK51_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP6432037.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1214 c         1442 g         2233 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaacaaatta gtcgctcctt tgatcagaag ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt cccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACCDF010000104         7401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCDF010000104 DACCDF010000000
VERSION     DACCDF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.110"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            69..152
                     /locus_tag="IVK51_002486"
     CDS             69..152
                     /locus_tag="IVK51_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6432038.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            277..1014
                     /gene="erm(B)"
                     /locus_tag="IVK51_002487"
     CDS             277..1014
                     /gene="erm(B)"
                     /locus_tag="IVK51_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6432039.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1184..1444
                     /locus_tag="IVK51_002488"
                     /pseudo
     CDS             1184..1444
                     /locus_tag="IVK51_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1607..2272
                     /locus_tag="IVK51_002489"
                     /pseudo
     CDS             1607..2272
                     /locus_tag="IVK51_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2308..2553
                     /locus_tag="IVK51_002490"
     CDS             2308..2553
                     /locus_tag="IVK51_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6432040.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2656..3525
                     /locus_tag="IVK51_002491"
     CDS             2656..3525
                     /locus_tag="IVK51_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6432041.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3506..4240
                     /locus_tag="IVK51_002492"
     CDS             3506..4240
                     /locus_tag="IVK51_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6432042.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4273..5185
                     /gene="ant(6)"
                     /locus_tag="IVK51_002493"
                     /pseudo
     CDS             4273..5185
                     /gene="ant(6)"
                     /locus_tag="IVK51_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5182..5724
                     /gene="sat4"
                     /locus_tag="IVK51_002494"
     CDS             5182..5724
                     /gene="sat4"
                     /locus_tag="IVK51_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6432043.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5817..6611
                     /locus_tag="IVK51_002495"
                     /note="aph(3')-IIIa"
     CDS             5817..6611
                     /locus_tag="IVK51_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6432044.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6719..6907)
                     /locus_tag="IVK51_002496"
                     /pseudo
     CDS             complement(6719..6907)
                     /locus_tag="IVK51_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6887..>7401
                     /locus_tag="IVK51_002497"
     CDS             6887..>7401
                     /locus_tag="IVK51_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6432045.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMI"
BASE COUNT         2593 a         1119 c         1555 g         2134 t
ORIGIN      
        1 ttgtgtataa taggaattga agttaaatta gatgctaaaa atttgtaatt aagaaggagg
       61 gattcgtcat gttggtattc caaatgcgta atgtagataa aacatctact gttttgaaac
      121 agactaaaaa cagtgattac gcagataaat aaatacgtta gattaattcc taccagtgac
      181 taatcttatg actttttaaa cagataacta aaattacaaa caaatcgttt aacttctgta
      241 tttatttaca gatgtaataa cttcaggagt gattacatga acaaaaatat aaaatattct
      301 caaaactttt taacgagtga aaaagtactc aaccaaataa taaaacaatt gaatttaaaa
      361 gaaaccgata ccgtttacga aattggaaca ggtaaagggc atttaacgac gaaactggct
      421 aaaataagta aacaggtaac gtctattgaa ttagacagtc atctattcaa cttatcgtca
      481 gaaaaattaa aactgaacat tcgtgtcact ttaattcacc aagatattct acagtttcaa
      541 ttccctaaca aacagaggta taaaattgtt gggagtattc cttaccattt aagcacacaa
      601 attattaaaa aagtggtttt tgaaagccat gcgtctgaca tctatctgat tgttgaagaa
      661 ggattctaca agcgtacctt ggatattcac cgaacactag ggttgctctt gcacactcaa
      721 gtctcgattc agcaattgct taagctgcca gcggaatgct ttcatcctaa accaaaagta
      781 aacagtgtct taataaaact tacccgccat accacagatg ttccagataa atattggaag
      841 ctatatacgt actttgtttc aaaatgggtc aatcgagaat atcgtcaact gtttactaaa
      901 aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca atttaagtac cgttacttat
      961 gagcaagtat tgtctatttt taatagttat ctattattta acgggaggaa ataattctat
     1021 gagtcgcttt tttaaatttg gaaagttaca cgttactaaa gggaatggag ataaattatt
     1081 agatatacta ctgacagctt ccaagaagct aaagaggtcc ctagcgccta cggggaattt
     1141 gtatcgataa ggggtacaaa ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc
     1201 taagtgtggc aacaatattg tattaaaaaa atcgttttat ggttgttcaa attatcctga
     1261 atgtaagttt actttagctg aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa
     1321 agaattactg gagggaaaag aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc
     1381 ctacaatgcc gttgtaaaaa ttggggaaaa gggatatatt gattttatct ctttttcaaa
     1441 ataaacatgg ggctgaccca aaagtagctc ctatatgaaa aatagaggtc tttccattta
     1501 gatagtagtg gaaagacctc tatttatctt ttgaaagtga atacaaaaat aaagcacact
     1561 ctgctataat ataggtgatt aatccatttg agagaggtgc tttatcatgt atcaaaatta
     1621 taccataaac caactttgtt tgcctataga tcttgaaatt aaattagaag aaaatgattt
     1681 tgctcatgcc attgtgcagt ttgttgactc tattcccgat gaagtctttc ttccttatta
     1741 tcaatctatg ggtagacccc aatatcatcc tcgtatgcta ctttccatta ttctttgtgc
     1801 ctatattcaa ggggtttatt cgggccgcca aatacaaaat atgctgatag atagtattcg
     1861 gatgcgctac ttatctcaag agcaattccc taactttcgt actattaatc gttttcgcgt
     1921 tcatccgatt atgaacgata ttctagatca ttcatttgtt caatttcgtg agctactggt
     1981 tcaaagtggc ttaatcagtg gcagtgctct atacattgat ggtacaaaaa ttgaagcgga
     2041 tgcgaataaa tactctttcg tttggaaaaa aagtatttta aagtataagg atagccttga
     2101 tcaaaaagcc cttgaaaact accaacaaat ggtggaaaca aaaatcttac ctgagttaat
     2161 agatgattta aaggatgaac tttctataga agagattgaa aggattcgtc aatctttgga
     2221 agaaaaggaa aatcaattaa ttgaagcgat tgaccaaacc gaaacagtag aagggggctg
     2281 acccattatt aataaggagt ggacaacatg attgatgtaa atgagctatt ggaagaagct
     2341 attagagaaa ctgaaaatct aaatgatggc gaagtttttc ttgttaaaga tttattcaag
     2401 ggatatgtgg ggaataggat acccagaaag gatcgacttc tgcttggaac gttattttta
     2461 aaccatgtta gcaaaatgaa tggtaattta aaggctattg aaaagacttc atctaatcag
     2521 cagaggtata aaaagacaat tgataagttt taactatctt ttgtgaaatt aaaaaaatta
     2581 taaatatgtt gagtagaagc aaaagatttg gcttggaatt tttacagcag tgaatcgata
     2641 aaaggagcat gcttgatggt agataatatt attaaatcag tagcagagaa attatcctct
     2701 ctgtcttata tagaaggtat tgttttaggt ggttcacgtg caaggggcac ccatacagag
     2761 gattcggata tagatatcgg catctattac aattcagaat catttgacat aaatactatt
     2821 aatcaattcg ctacaaagct ggatgatgag catagaaata accttgttgt acctcccgga
     2881 gcatggggtg attggattaa tggcggcgga tggttagtca taaacgggta tcatgtggat
     2941 ttaattttac gtgatattaa acgtgtggaa caaataatga aagatacaga gcacggaatt
     3001 gttactgcca attatcagac tgggcatccc catggttata ttagtgcaat gtatcgagga
     3061 gaattagcga ttagcaaaat actatatgct aagaatgaaa gcttatgcga attaaaaaaa
     3121 caggcagaaa cttatcccaa tgctttgcag aaaagtttag ttaacttttt tatgtttgaa
     3181 gcagggttct ctttaatgtt tgtaaaagca aattcgggaa cagacgataa atattatatt
     3241 gcgggtcatg tttttcgtat agtttcatgt ttaaatcaag tgttatttgc atgtaataat
     3301 gcttattgta tcaacgaaaa gaaagctata aaactgcttg aaacttttga acataaacct
     3361 gaaaaatata ccgagaaggt aaatcatatt tttgaagtac tcggtatctc actttttgaa
     3421 tgctacgaca tgaccgagaa gctttataat gaagtgaatg aaattgtatc ggagataaat
     3481 aactttttaa acgaggagag ttcagatgaa agaaaacaaa tatgatgata atatattttt
     3541 tcaaaaatac agtcaaatga gtcgctcgca gaaaggactg gctggtgcgg gagaatggga
     3601 gactttgaaa aagatgctac ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg
     3661 ctatggatgg cactgtatat atgcgatgga aaacggtgct tcctctgtag taggtgttga
     3721 tatttctcat aaaatgctcg aagtagcaaa aggaaaaacc cattttccac agattgaata
     3781 tgaatgctgt gccatagaag atgtggattt cccagaggag agctttgatg taatactaag
     3841 ttcgcttgcg tttcattatg tagcagacta tgagaattta ataaaaaaga tatataggat
     3901 gctgaaggct ggtggcaatt tagtttttac agttgaacat cctgttttta ctgctcatgg
     3961 aacacaagac tggtattata acgaaaaagg agaaatactg catttcccgg tggacaatta
     4021 ttattatgag ggcaaacgga cagctatgtt tttggaagaa aaggttacaa aatatcatag
     4081 aacactgacc acatatctaa atacactgct ttcaaatagt tttataataa atcagattgt
     4141 ggagccacag ccgccagaga acatgatgga tattccgggg atggcggatg aaatgcgacg
     4201 cccaatgatg ctgattgtat cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg
     4261 aggagtatgt aaatgagatc agaaaaagaa atgatggatt tagtactttc tttagcagaa
     4321 caggatgaac gaacgtattc gaattgtgac ccttgagggg tcacgcgcaa atattaatat
     4381 acctaaagat gaatttcagg attatgatat tacatatttt gtaagtgata tagaaccgtt
     4441 tatatctaat gatgactggc ttaatcaatt tgggaatata ataatgatgc aaaagccgga
     4501 ggatatggaa ttattcccac ctgaagaaaa gggattttcc tatcttatgc tatttgatga
     4561 ttacaataaa attgatctta ccttattgcc cttggaagag ttagataatt acctaaaggg
     4621 cgataaatta ataaaggttc taattgataa agattgtaga attaaaaggg acatagttcc
     4681 gactgatata gattatcatg taagaaagcc aagcgcaagg gagtatgatg attgctgcaa
     4741 tgaattttgg aatgtaacac cttatgttat taaaggattg tgccgtaagg aaattttatt
     4801 tgctattgat cattttaatc agattgttcg ccatgagctg ctgagaatga tatcatggaa
     4861 ggtcggcatc gaaacaggct ttaaattaag tgtaggcaag aactataagt ttattgaaag
     4921 gtatatatcc gaggatttgt gggagaaact tttgtccacc taccggatgg attcctatga
     4981 aaacatatgg gaagcattat ttctatgcca tcaattgttc agggcggtat ccggtgaggt
     5041 ggcggaaagg cttcattatg cctatccgga gtatgatagg aatataacaa aatataccag
     5101 ggacatgtat aaaaaataca ctggtaaaac cggctgcctg gatagcacat atgccgctga
     5161 tatagaagag aggcgggaac agtgattaca gaaatgaaag cagggcacct gaaagatatc
     5221 gataaaccca gcgaaccatt tgaggtgata ggtaagatta taccgaggta tgaaaacgag
     5281 aattggacct ttacagaatt actctatgaa gcgccatatt taaaaagcta ccaagacgaa
     5341 gaggatgaag aggatgagga ggcagattgc cttgaatata ttgacaatac tgataagata
     5401 atatatcttt actaccaaga cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg
     5461 aaccggtacg cttatataga agatatcgcc gtatgtaagg atttcagggg gcaaggcata
     5521 ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc ataaaaactt gcatggacta
     5581 atgcttgaaa cccaggacaa taaccttata gcttgtaaat tctatcataa ttgtggtttc
     5641 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
     5701 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
     5761 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
     5821 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
     5881 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
     5941 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
     6001 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
     6061 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
     6121 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
     6181 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
     6241 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
     6301 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
     6361 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
     6421 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
     6481 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
     6541 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
     6601 aattgtttta gtacctagat ttagatgtct aaaaagcttt aactacaagc tttttagaca
     6661 tctaatcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     6721 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     6781 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     6841 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     6901 cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat
     6961 tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga
     7021 agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct
     7081 acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt
     7141 tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat
     7201 caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa
     7261 cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg cagcaattca
     7321 gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga
     7381 accggaaagc agaatgatag a
//
LOCUS       DACCDF010000105         7202 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCDF010000105 DACCDF010000000
VERSION     DACCDF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.111"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(401..718)
                     /locus_tag="IVK51_002498"
     CDS             complement(401..718)
                     /locus_tag="IVK51_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6432046.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(719..973)
                     /locus_tag="IVK51_002499"
     CDS             complement(719..973)
                     /locus_tag="IVK51_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6432047.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1332..1736
                     /locus_tag="IVK51_002500"
     CDS             1332..1736
                     /locus_tag="IVK51_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP6432048.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            1753..2901
                     /gene="tnpB"
                     /locus_tag="IVK51_002501"
     CDS             1753..2901
                     /gene="tnpB"
                     /locus_tag="IVK51_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP6432049.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(3124..3342)
                     /locus_tag="IVK51_002502"
                     /pseudo
     CDS             complement(3124..3342)
                     /locus_tag="IVK51_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3608..3802)
                     /locus_tag="IVK51_002503"
     CDS             complement(3608..3802)
                     /locus_tag="IVK51_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432050.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            5154..6194
                     /locus_tag="IVK51_002504"
     CDS             5154..6194
                     /locus_tag="IVK51_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6432051.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            7018..>7202
                     /locus_tag="IVK51_002505"
     CDS             7018..>7202
                     /locus_tag="IVK51_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6432052.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQ"
BASE COUNT         2556 a         1114 c         1142 g         2390 t
ORIGIN      
        1 ccatagatat attggacttc ctcttttaaa acacaaaaat aaatactacc ttatttaggt
       61 agtatttatt aaaagttacg tcaattttga ttaaaaatat actacttgaa cctctcatga
      121 gtaaagtcac gaaatttttg ggaatagagc gtacttgata tcttattttt tcactaatag
      181 cagtgtctct tattcaccaa gcgatccccg tagttcctac ggttcttaat ttttctctag
      241 actaatgatt gtatctttag accttcggcc ataacatgga gcaaaataaa aaggtactaa
      301 gtcttatata tcaatcttcg agtaaccgac ggttaacaac catatcgagc ggcattaatc
      361 acccacctgt agaggatgag cgactttttc ctaattattg ttaaaagcct agtttcattt
      421 gtttagattg gaaaatacgt cctacaagta tctctttttt ttcatggtcg atccgataaa
      481 ataaggcata gcttttgccg atcacgatcc gatatgttgg gatatccatt ccatagattt
      541 cggaaacttc ctcatgcata gttggaaaat ttttggttag ttctaaagcc ttctggatcg
      601 cacctacaaa tctttttatg ctggctggag aaagagcaag ctcattttcc cattcttcta
      661 ttatcgtttc aaggctggtc tgaaaacttt tagaataaac tagcgtgtac tctttcatct
      721 attcactcca gccgtatttt ccgaaagctt ttaccatttc aatttctgaa actgttaaac
      781 ctttctcaaa gtcttggaaa ctttcttcaa tcaatttgcg atcgtgttct tttgctattt
      841 gagtagtatc taatatgaat aagttgtctt cttgtttggc ttggataatg tctccgcttt
      901 ttaatccaag agagcttgga aggatcactc ccacgctatt gccgatcttt ctaaccttta
      961 gttcttttcc catgttatca cactcctttt agtttttgat gaataaatta taacatgtta
     1021 taacaacaat tgccaacggt gtttcttaag ggattccact ccatattatt attggacaaa
     1081 ataagccact tagaacgctg aaaacatata caaacgatat caaaatacga gtgaactact
     1141 caccatacac ttccgttaac atccatttaa cagaattagg cagaagtcgc ccatcctcta
     1201 taggtgggtg atgaatgccg ttcggtatga gggttaccgt tggtaactcg aagaccgaca
     1261 actaaggcgt attgcctttt tattttgtcc catgttacaa tcagtctata caagaaatga
     1321 gttgatataa catggaatta gatacaaata atcattcagt atttcttctt tattaccacc
     1381 ttgttttagt aacgaaatat cgtagacaag tgattgatga tgaaatatct gattatgcca
     1441 aaactacttt tgaaagaatt tcagaatcgt atcacattac tttagtggag tggaatcatg
     1501 ataaagacca cgttcatatt atgttcaaag ctcaacctaa aacagaattg acaaaattca
     1561 tcaatgccta taaaagtgcc agctcaagat tgataaaacg tgactttcca agagtcaaac
     1621 aatttctttg gaaagaaatg ttctggtcta aaagcttttg tcttctgaca actggtggcg
     1681 caccaattga tgtgattaag aagtatatcc aaaatcaagg aaacaatcat aaatagaaag
     1741 taggtgaact ttatggaaca actaaaagca tacaaattca gaatctatcc aacagaggaa
     1801 caagaaatct tctttgccaa atcttttggt tgtgtccgta aggtctacaa tctaatgctt
     1861 gatgaccgaa aaaaggcgta tgaagaagtt aaaaatgatt cttctaaaaa aatgactttt
     1921 ccgacaccag ctaaatacaa gaaagaattt ccgtttttga aagaaattga tagccttgct
     1981 ttagccaatg cccaacttaa tttagataaa gcatacaaga atttttttcg ggataaatcc
     2041 gttggatttc cacgtttcaa aagtaagaaa aatcctgttc agagttatac aacgaataat
     2101 caaaatggta caattgcttt gattgatagt aaattcatca aagttcctaa attgaagtca
     2161 ttagtaagaa ttaagcttca tagacaacca aaagggatta tcaaatctgc tacaatatca
     2221 cgtcattcta gcggtaaata ctatatttct ttgttgtgta aagaagaaat tatcgaatta
     2281 cctaaaacta attccacaat tggtattgac ttaggtatta tggactttgc gattctttct
     2341 gatggacaaa aaattgataa taataaattc acgtccaaaa tggaaaagaa actaaagcgt
     2401 gaacaacgca aattgtctag gcgtgcttta ttggctaaaa ataaaggtac caatctattt
     2461 gaagctaaaa attatcaaaa acaaaaacgc aaagtagcta gattgcatga aaaagtaatg
     2521 aatcaacgta ctgattttct gaataagttg agtacagaaa tgatcaaaaa tcacgatatt
     2581 atctgtattg aagacttaaa cacaaaaggt atgttgcgaa atcataaatt agctaaaagt
     2641 atttctgatg tttcttggtc aaagtttgtg actaaattac aatacaaggc tgactggtat
     2701 ggacgaaaaa tcatcaaagt agataaatgg tttccgtcta gtcagatttg ttcagagtgc
     2761 ggacataaag atggcaagaa atctcttgaa attcgagaat ggacttgtcc tgtttgtcat
     2821 actcatcatg atcgtgatat caatgctagt atcaatattc tgaccgaagg tctaagacta
     2881 cattctatgg gattggctta gatccaatca tcaagaaccg taggaactac ggggatagct
     2941 tggtaaataa gagacaccgc tgttagtaaa gaaatacgct atcaagtatg ctctattccc
     3001 aagaagctcc cacttcaagc gttaagaacc gtcaggttta gctaagtggt gagtagttca
     3061 cgtaggtatt tttattatat atagatttaa catatgtcgg tcggttgatt gatatagcta
     3121 aagttataac tcaaccgaag ccgattcatc tacacgaata aagttgcgag tgttctcggc
     3181 taattcataa aaattgttat gtgttccata tagcaatact ggaataactt tattttcttg
     3241 aatctcataa gcaactctat acacggtgac cgcatactta aatccattag accaattacc
     3301 tgataaacct ccagtttttt gctcttactt aaatggatca atggcaattt catcacaaac
     3361 ctatagagga tgagtgactt ctgcctaatt acgttaaaaa atattgaaag taactctatg
     3421 tagcaaaaaa tatctagaat ttcatttcct caaaactgat cgatattgta tctaaaaata
     3481 tatactacct aaaataggta gtatatatta aaatatgtgc ttttgttaga aaaatcaata
     3541 cataaattat catatctgta tatgtcattt aggtatttca gttatgttat aatagttttg
     3601 ctgtctgcta ccccggccta atctggcaag gggaggagga gatataatga aatacctttt
     3661 tgattttgtt tttgcaatca agccacttgc agatgtagtg tgcagttacc tctgcaaatg
     3721 gcttgattgc aaaattgcca aaaaagacag taagcctacc cgttaattct ataatataga
     3781 aacgtaaaaa aaaccccatc agcctgcacg ctgatggggt ttggtgatat aatgaaatac
     3841 ctctttgtta ctcataattg taccataatt agtcacgaat gcaaccaata gaaaaaacta
     3901 cttagaacgg tagatcatcg ctaataacaa tgcattgtcc accaaatgga ttttgtgaag
     3961 cattaaatac attgttatta attgtcgctg cttttaacta ggcctctccc cttctattat
     4021 aaaagaagat atatatcatt tcttaattac ctactacctt aaaccaaaat ttaagtacta
     4081 cctaatttaa gtattactta aacaatctaa aaaatagtta tcccaattgt tatgctttta
     4141 taatacgatt atttgtaaca aaatgaatat tatataatta aacactacca tatttaggta
     4201 gtgtttaatt attagcaaaa caaaaaatat ttattctatt ctcttaactt caactaagtt
     4261 atgctgcaca cctttatata aatcatatgc aactcattag ctagacaaac tacaagcatg
     4321 tagttttatc tgttaattat gtactaccta aataaagtag tttataatta gcaagtatat
     4381 atatttaaaa atttaagatt aattttttta tgacttgtac aaatttatct gatattctct
     4441 tattaagaac tacctaattt gggtagttct tattttttac attttaagta gtatttaatt
     4501 gcaatttggt agtatttttt tattatctta ttcttagaac ggtagtactt aatataaata
     4561 taggtagtat ctaatttaag tattaatggt agtatctaaa ttaacaggta gtatgtaact
     4621 actttaaagt aatttttttc agattaacac agtagtatct tattaaataa gttaatttta
     4681 atggtagtac ataattatta tatatatctg acattggtag tacttattgt attttttagt
     4741 agtatataat ctattgtttt ctagcctaaa gccttgtctt ataagaataa tataatatat
     4801 actactgaaa taaggtagta tatattacta gtaatggtag tatgttttat atgcataagg
     4861 tagttcttaa tttatttttt gttttgacac tggtagtact taattatctt taattttaaa
     4921 actaaatttt attcatacgt ttttctggta gtatttaatt ataaataata tttatttaaa
     4981 gtagttctta atgactttaa aggtagtaca taattcattt tatttctgat tttaggtagt
     5041 atttaattaa aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga
     5101 aaagagtagg taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg
     5161 attttaaatt ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag
     5221 tctttattgt ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc
     5281 ttttaaaagc aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg
     5341 tttattttac ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag
     5401 cgatagcaat caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat
     5461 ttaacaaaca gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct
     5521 cagaaaatga tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata
     5581 aatcagaggg tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg
     5641 gggctcaaga gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga
     5701 aaatcatacc agaacttaat aataatatat tagacactaa tagacacaat atagacactg
     5761 aaaaagaccg cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg
     5821 acagcattgc tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa
     5881 gttacagtga agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa
     5941 tagaaagtgg tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac
     6001 aagaattata cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa
     6061 atatccagaa cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg
     6121 ctaaagtatc aagtgaaaaa cgtttgaatt atgaaagctc cccaagcact attacgaaag
     6181 actggttaga gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa
     6241 ttaaaaattt tttattattc taattatatt acgaatatta caaaataata gctattttaa
     6301 tagaatttat aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa
     6361 gtgttaaaag aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct
     6421 tttttttgag ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa
     6481 atcttgatat tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa
     6541 atttaattgt ctgctatgct tgtgatgaat tgattttcgt tctgcacttt tggatgataa
     6601 atttggacaa ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac
     6661 cttcgtcagc gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg
     6721 ttacggtatt acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt
     6781 ttggctgtgt taaggttaaa aatacgtgat attacgatga atctatgcct aagcacaatg
     6841 ttataaaaat gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc
     6901 atgtttggat gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt
     6961 gaaaaataac aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg
     7021 tcatatacag gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct
     7081 acagagaaaa tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag
     7141 aaatatataa tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa
     7201 aa
//
LOCUS       DACCDF010000106         7187 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCDF010000106 DACCDF010000000
VERSION     DACCDF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.112"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK51_002506"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IVK51_002506"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP6432053.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IVK51_002507"
     CDS             complement(1172..1867)
                     /locus_tag="IVK51_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP6432054.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IVK51_002508"
     CDS             complement(1880..2596)
                     /locus_tag="IVK51_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP6432055.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSILYK"
     gene            2926..3534
                     /locus_tag="IVK51_002509"
     CDS             2926..3534
                     /locus_tag="IVK51_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP6432056.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IVK51_002510"
     CDS             3859..4182
                     /locus_tag="IVK51_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6432057.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IVK51_002511"
     CDS             4364..5734
                     /locus_tag="IVK51_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP6432058.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            6081..>7187
                     /locus_tag="IVK51_002512"
     CDS             6081..>7187
                     /locus_tag="IVK51_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6432059.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKK"
BASE COUNT         2110 a         1445 c         1343 g         2289 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcgttatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcgaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttatata aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaacat actaagatta gaggggaaca tcattgacga atgatgttct cctctatttt
     5881 ctatttttag attatagtga agagtgaaag aaagttcgaa caaagccttg aggactacga
     5941 gtccctatca aaaaccgaac ttctttactg tcattttaga atcaggttta cgtatgttgg
     6001 ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa gcagtaaagt tctttcaaaa
     6061 cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt agatgtttca atgggacaca
     6121 gctacagtgt cctatacaaa gacgacacct gcttatttga agacgagatt gaacacactc
     6181 aaagaggatt tcatgcatta ctcgaagaga tccagcactt gcctgagcgt ccattgattg
     6241 tttttgaagc aacggggatt tattccagac cagtcgaaaa gttctttcaa gatcatcact
     6301 tttcttactg tttgttaaac ccgttagagg cgaaaaaaca gatggaagaa agcaccttac
     6361 gcagttggaa aacagataaa tcggatgccc accggctcgc acagactcat cttaaaaatc
     6421 aacgtcagcc aaaagaagta caaagcaatt tttacttaga gatgcgcgat ttagcccgtt
     6481 tttatcaaga aattgagaag aaaatcactc gtctgcgcat ggatttacac aactgccttc
     6541 aattgacttt tccagagctt gaacagttct tttcaaacag gctgacccct tacgcattga
     6601 cattgatccg tctttttccc catccagatt ttgtattggc atctactcgg acaaaaataa
     6661 aaaataaact gatcaacgaa acacggaaaa aaatctccgc gaatcgtgcc gagcaaaagg
     6721 cagatcaaat catccactat gcacagtgtg cctatccagc tgttgaaaaa gacagtatcc
     6781 actgtcaaaa aacgatctat tacgccgaac ttctccaaga tttactcgaa caaaaaaaag
     6841 cactggcgac acaaatgatc aaaaaagccg aaggctctcc ttgctttttg ttgtaccaaa
     6901 cgttccctgg gattggcgca ctcacagccg ctttattgct cggtgaatta ggagatatca
     6961 cacgatttaa aacccataaa caattgaatg cctttgtcgg aatcgacatc cgacggtatc
     7021 attcaggtaa gtatactggc caagaccgca tcaataagcg tggtaatccc aaagcacgga
     7081 aaattatttt cttcaccatt cgaaatatga ttcgccagca acgcgcagct cctaatcata
     7141 ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc taagaaa
//
LOCUS       DACCDF010000107         6644 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCDF010000107 DACCDF010000000
VERSION     DACCDF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.113"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1..859)
                     /locus_tag="IVK51_002513"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IVK51_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IVK51_002514"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IVK51_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP6432060.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IVK51_002515"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IVK51_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IVK51_002516"
     CDS             complement(2143..2373)
                     /locus_tag="IVK51_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP6432061.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IVK51_002517"
     CDS             complement(2603..3421)
                     /locus_tag="IVK51_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP6432062.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IVK51_002518"
     CDS             3582..4271
                     /locus_tag="IVK51_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP6432063.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IVK51_002519"
     CDS             4285..5787
                     /locus_tag="IVK51_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6432064.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IVK51_002520"
     CDS             5800..6276
                     /locus_tag="IVK51_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP6432065.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IVK51_002521"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IVK51_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DACCDF010000108         6635 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCDF010000108 DACCDF010000000
VERSION     DACCDF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.114"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            99..746
                     /locus_tag="IVK51_002522"
     CDS             99..746
                     /locus_tag="IVK51_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP6432066.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVK51_002523"
     CDS             1139..3784
                     /locus_tag="IVK51_002523"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP6432067.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVK51_002524"
     CDS             4094..5407
                     /locus_tag="IVK51_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP6432068.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVK51_002525"
     CDS             5397..6050
                     /locus_tag="IVK51_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6432069.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..>6635
                     /gene="radC"
                     /locus_tag="IVK51_002526"
     CDS             6105..>6635
                     /gene="radC"
                     /locus_tag="IVK51_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HAP6432070.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHN"
BASE COUNT         2182 a         1132 c         1455 g         1866 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataat
//
LOCUS       DACCDF010000109         6452 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCDF010000109 DACCDF010000000
VERSION     DACCDF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.115"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(95..550)
                     /locus_tag="IVK51_002527"
     CDS             complement(95..550)
                     /locus_tag="IVK51_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432071.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(704..1912)
                     /locus_tag="IVK51_002528"
     CDS             complement(704..1912)
                     /locus_tag="IVK51_002528"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP6432072.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2024..2356)
                     /locus_tag="IVK51_002529"
     CDS             complement(2024..2356)
                     /locus_tag="IVK51_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP6432073.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2504..3379
                     /locus_tag="IVK51_002530"
     CDS             2504..3379
                     /locus_tag="IVK51_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP6432074.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3461..4042)
                     /locus_tag="IVK51_002531"
     CDS             complement(3461..4042)
                     /locus_tag="IVK51_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP6432075.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4048..5307)
                     /locus_tag="IVK51_002532"
     CDS             complement(4048..5307)
                     /locus_tag="IVK51_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP6432076.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5309..6355)
                     /locus_tag="IVK51_002533"
     CDS             complement(5309..6355)
                     /locus_tag="IVK51_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432077.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
BASE COUNT         1798 a         1334 c         1100 g         2220 t
ORIGIN      
        1 tcccttattc tgaataaacg gtgtggaaga gacagcctat cagtagtaat aagacacatc
       61 tctttcacag cctcattttt gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat
      121 cttctacacg ttgatctgga ttgatcacga tatacaagtt acctgttttt tcgctgattt
      181 ttgttgattg atattgattg tctaatcctt ctgttccttt atctagatat gggaagtaca
      241 caagatcagg tgtgccattt cggtaaagga tttccattcg ctcgatatct tcgtatttca
      301 atgcgcgatt aaacatgccc atttccagtc cgcctaggtt gatatcatgt gtcgcaactg
      361 cgtccccttc atggaagatt tcgattttaa atccagcaca cggatggatt tcaacaaatt
      421 cactgccatt gattcgtcca aaacttgttg tgatttgttt gatccaaaga tcgccgatat
      481 gacggcggtt gatcgtccag gtttctccat ttctaaaata aaattttagc gctgtcattt
      541 ctttactcat taaaaagact cctttatgat gtaatttatt cttgtactac ctcagtttag
      601 cacaaattta tgtaagtggt cacatcttat gttttttctt ctaatgacgt atctgcccac
      661 tttaagtagc caacaaagct gagcagatac agtctagtgt tttttaaatc aatttttctt
      721 tcgataaaaa atccaaaacc tgttttttat cattttttat tctttgttct acgacggctt
      781 tttcagctaa ttgcaaagct tcactaaccc acttaccagg tttttttcca gtttcttcta
      841 gcaagatttt accatctact gctaattcgc ttaatgactt gataggtaaa cgatcatagg
      901 attctcctac ggcatccatc tgaggtgttt cgtcaaaata gatcatcaat tcttctactt
      961 gcagtgcatg ttttttgcct aagaagtaca attcgaatgg ttccaaagct ccagtttctc
     1021 gttgtcgtag tccttggaca agtgcttgaa cgtctttaat catttggtta gaacacttcc
     1081 acgatttcaa aaaggaacga atctcatttt cttttagatc cagttggctg atcaataatg
     1141 cccatgcttg gctctcttct ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg
     1201 cttcgccttc ttctttcaaa ccgggacagc aaatgtagca ctctgtttca ataaatgcat
     1261 ggattcccgc ggcccgttca tttcccaaga gcatcttgat gaattccaca tttatacgtt
     1321 ctacagatat tttacctaat agctcgtggt tttctttgat ggcgtagaag gtttcttcct
     1381 ctaatttgaa ccctagttga ctgacaaaac gcaacccgcg catcattcgt aaggcatctt
     1441 catggaaccg ctcatgagga ttgcctacag ctcgcaagat ctgttcttct agatcttgca
     1501 gaccatcgaa caaatcgatg atttctccat cttctttcaa agcaaacgca ttgatcgtaa
     1561 aatctcttct tttcaaatct tctgccaacg aacgaacgaa ttcgacatga tctggtctac
     1621 ggtagtcctg ataagttgat tccgtccgaa atgtggtgat ctcatattgt tcatcatttt
     1681 ctaataccaa cacagttcca tgttcgatcc caatatctat cgtttttgga aaaagctgtt
     1741 tgatttcttc tggaaatgcg cttgttgcga tatccacatc atggattggc tggttcagca
     1801 agatatcacg aacacttcca cctacaaaat aagcttcata tcctgcattc tctaattttt
     1861 tcagaactgg aattgccttt tggtattcca gaggtaattg ttttaatttc atttttccaa
     1921 cctttcctgc gtgtttcttg tcctgagata aagactcagt tgcatggtga ctgtttgaca
     1981 agtccgtttc agagtcttca aggccaggac cacttcatct tgtttactcg tccttacgct
     2041 caaagcgatt atggtctctt tgattgaatt tttccatgtt tttttcaaaa acttcagtta
     2101 agtcgatatt caatgaatta gccatgatca tcgtaacgaa taaaacatca ccaagttctt
     2161 ctgctaccga gttaactggt tcagaagcct ttttgctttt ttctccataa gtgtggttga
     2221 cttctcttgc caattcacct acttcttcag ttaatcgagc catctgagct aatggagaga
     2281 aataccccac cttgaattgc tggatatatt catccacttc ctgctgcata ctgtataaag
     2341 aacgcttttt atccatcttt ttccctccaa aactatctta ccaaaacttt aacgcaaatc
     2401 ctagataaga gcaattgaga aaatcgaatt tccatgctac atttaggaag gaatttctaa
     2461 atggaaattc tgtgaaaata cataattgaa agctggaatt catatggtca aacgaaaaac
     2521 agtaaaagaa gttcttttta tcattatagg aacaagtatt tatgcatttg gtcttgttta
     2581 cttgaacatc gctaatcatt tagcagaagg gggagtctca gggatcacct tgatcttaag
     2641 agctttattt ggtattgatc cagcttatac gactttatta atcaacatcc cattgatttt
     2701 actaggagga aagatcctcg gtaaacgttc cttggcctat actgttctag gtacactttc
     2761 tttgtcattc tttttatgga tctggcaaag ggttccacta gaaatcaatt tacagcatga
     2821 tctactgatc gtttctctac ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta
     2881 ccgcatgggt ggaacaacag gcggcagtga tattatcgct cggattttag aacaaaatta
     2941 cggcatcact atggggcgtt ccctattggc atttgatatc atcgtcttac ttgcttcatt
     3001 aacctatatc gacttgaaac gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg
     3061 tgtgattgat tcgatcttag atggcggata ttctgcaaaa ggtctacttg tcgtctccaa
     3121 caaaagcgaa gagattgcac cactactgat gaccggtctg caaagaggtg tgactttcct
     3181 ttctggagaa ggcgcattct ctggtaattc aaaaaaaatc atctatatgg tcgttagctc
     3241 tagtgaatta actgaagcta agaggatcgt ccacgagatt gacgaaaaag ctttcctttc
     3301 aatcatcaat gtgcatgaag tggaagggca aggctttact tatttgaagc cacagacaag
     3361 attactgaaa aaactataag aaaaaagaca aagataggct atgaggaaag attccacatc
     3421 cagctgacct ttgtcttttt tagtactata ctatttttaa ctaattgtta tgttcactta
     3481 cccggttact tttcttttgt gtctgcaaaa ggatctttcg gttaacttca tcagataatt
     3541 tcaaaaaagc atccctatct ccatcttcta gtgcttgatc gatttgagca taaagcattt
     3601 ttagttttgc ctcttcttct ttttcttgaa aatatcgttc aattaattca gtatcttctt
     3661 cactgactga agaatcccaa gaagaaaatg gattgtcttc taagatagat agatattctc
     3721 ttgtctgcca agcattttcg aatatacatt ccacatataa aggctttttc caattcaaac
     3781 ggatttcatg aaaaatctgg tctgtgtccg taaattcttt tccctgcaaa aacaatttca
     3841 tgggttcccc ttcaacagat agatcactga tctgcagtcc tcgatccgca aaattcgccc
     3901 cttcaacaaa gtggacattg cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc
     3961 aaaaaacttc tcttcggcta aaagatacat gattgaccat ccacgttaaa aaattcttct
     4021 tttcccgtac atcaattagc atttttctca cctctctgtt aggtcatcta ataatgacag
     4081 tgcctccata tctcctggtt caagtgttaa ataataagtc aataattctt gcgctctttt
     4141 cagctgccct tcttctctta agaaaaaggc atactctttc aagaaatctg gttcatgtga
     4201 tagttcatga taagcctgtt catagtgtac agctgcaacg gtaaagtctt ctaattcatt
     4261 ataagcatgg gctaggttcc attctgcata aggattagca gtttcttcca tctgattgat
     4321 cacttgtatc acatcttcat atctttcatc atccaagtaa agattactta aagtaagtaa
     4381 tgtttcatct tgtttgtctc ctacttctaa agctttcagt aaaagttctt ctgctcgctg
     4441 tctatcatgc agacggaaag cattttccga ggccaggtga taaagttcga cttggtaagg
     4501 attttctttg attccttctt ctaacactgt ttgtgcttct tcaatcattt cttcttcttg
     4561 aagagcttgt cctagatata gatataacga ttgataatgc ggattaactt ctcgaagttc
     4621 ttccagatag ttgattgcct gctgattttc tttcaattga agattcgtaa aagctagctg
     4681 gaacagtcga tcatctgtcc tttcttcttt cagtgcttct tctaaaggta cgatagctgc
     4741 ttcgaattct ccttgcatac ttaaggattg tcctaaacgt tcctgcatcg agattcctga
     4801 aatttcattt atcccgcttg ccaacaatgt ttcataaacc actgctgcct ctttgaaacg
     4861 atcaactgaa aaatacaatt cagctagcgc aaactggatc aatggttcat caggaagcaa
     4921 cccagctgct tctttcaatt ttgcttcact cacttcaggg attccgataa cttggtacaa
     4981 atcagctatc gccaataagc tttgcggata gtaatcactt tgcttatcca ctttttccag
     5041 gtattcaaaa gctaagtcga tctcattatc ttcgattgca atttctgcta acgggatgtt
     5101 cagtccgtca ttttctgggt attggttgat cagttgttca aaaatctgtt ttgcttcgat
     5161 caaaaagcca atagccagca attcttcgcc tagttcagct aatatatctg ctgggtcttc
     5221 ttttaatgct ttttctaaaa agatttgtgc ttgtgacaag tcttcatctg ataaagcttg
     5281 caacattttt tcgctatttg tattcatttc aattccctct tatctcactg acgatttgtt
     5341 gaaataccgt cattgttctc aataattctt tttcgtttgg atgttggtat ccgtctatta
     5401 tacctatttt ttctaaaaca agtatttttt tgcttttttc tgccaaaaaa agaacttttt
     5461 ccaaatactc catctttgaa atctgttcca gccagtggat tggatagcca agcaagtcca
     5521 gccacacaaa aaagtttttt agttggaaat caaaaggata tgcttggaga ttccacacga
     5581 aatgaaacat cattcctaaa aattttttca tactagcaga aagtagatgg ccattttcgg
     5641 tcagataaaa agcttgttca aacagtttac cataatttga caggttttct gcctctaatt
     5701 gataaaaata gatcatttct tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt
     5761 tatttggata gtttttatac agattttgca aaaaacgatg atcacaaatc attccgcaac
     5821 agataaaagt caaaaagtca atctgctttc cgtccacctg ccgctcgctg attgtttgat
     5881 cataaacgat gcgttctggc agattgatgg tttgcagcac tacacgattt ggtagctgca
     5941 aaatcgtccg ctgcggtgtt aatgctcggc ttagagaacg gatcgataca gggatcatcc
     6001 atagtgatga atcaagggtt gtatgttttt gaaaaaaacc tgccagatcc atgatccctt
     6061 catttccaaa tccgatgata aacatggatt gattttcggg atagcgtttg acgaaatcaa
     6121 gcaggttctt taattctgtt aaatgattgc attgagtgtt ggcacaaata taccagtcat
     6181 tgtttgcttg atttgcaaat aacgggttga tcttatctgc aaacaaatca taatagcgct
     6241 gattcgttaa aaataagatt ggctgatcaa tggaaagttc tcttagctga ctgactagcg
     6301 tttctccgta aaacactttc gtcaacgcat cttctacttg ataatcaagt tccatacgca
     6361 tccctcctct tcttattttt attttaccat tagtaagcaa attctagaaa agataagagc
     6421 aaagctcacc tcataacttg gaaataaata aa
//
LOCUS       DACCDF010000110         6328 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCDF010000110 DACCDF010000000
VERSION     DACCDF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.116"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            127..579
                     /locus_tag="IVK51_002534"
     CDS             127..579
                     /locus_tag="IVK51_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432078.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IVK51_002535"
     CDS             593..1282
                     /locus_tag="IVK51_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432079.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IVK51_002536"
     CDS             1407..3758
                     /locus_tag="IVK51_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP6432080.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IVK51_002537"
     CDS             3838..4233
                     /locus_tag="IVK51_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432081.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IVK51_002538"
     CDS             4558..5175
                     /locus_tag="IVK51_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432082.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IVK51_002539"
     CDS             5216..5515
                     /locus_tag="IVK51_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432083.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IVK51_002540"
     CDS             5549..5716
                     /locus_tag="IVK51_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP6432084.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6324)
                     /locus_tag="IVK51_002541"
     CDS             complement(5761..6324)
                     /locus_tag="IVK51_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432085.1"
                     /translation="MIFKLRNRTEGLNIDKLFQEKASGKDTERPEFQKLLNYIREGDC
                     VIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLDTAMFDMFLSL
                     LSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLVFKTVVDMLKQ
                     GIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2171 a          878 c         1258 g         2021 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttcggt
     6301 tctgttgcga agtttgaaaa tcaaacgc
//
LOCUS       DACCDF010000111         6172 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCDF010000111 DACCDF010000000
VERSION     DACCDF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.117"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVK51_002542"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="IVK51_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP6432086.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="IVK51_002543"
     CDS             complement(2203..4389)
                     /locus_tag="IVK51_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP6432087.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVK51_002544"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="IVK51_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP6432088.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="IVK51_002545"
     CDS             complement(4611..5051)
                     /locus_tag="IVK51_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP6432089.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="IVK51_002546"
     CDS             complement(5125..5664)
                     /locus_tag="IVK51_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP6432090.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1598 a         1387 c         1088 g         2099 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccaacccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aa
//
LOCUS       DACCDF010000112         6100 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCDF010000112 DACCDF010000000
VERSION     DACCDF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.118"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            132..1106
                     /locus_tag="IVK51_002547"
     CDS             132..1106
                     /locus_tag="IVK51_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6432091.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1088..1549
                     /locus_tag="IVK51_002548"
     CDS             1088..1549
                     /locus_tag="IVK51_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP6432092.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1599..2063
                     /locus_tag="IVK51_002549"
     CDS             1599..2063
                     /locus_tag="IVK51_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6432093.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2077..2826
                     /locus_tag="IVK51_002550"
     CDS             2077..2826
                     /locus_tag="IVK51_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6432094.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2826..3656
                     /locus_tag="IVK51_002551"
     CDS             2826..3656
                     /locus_tag="IVK51_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP6432095.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3656..4303
                     /locus_tag="IVK51_002552"
     CDS             3656..4303
                     /locus_tag="IVK51_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP6432096.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKINYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4401..5177)
                     /locus_tag="IVK51_002553"
     CDS             complement(4401..5177)
                     /locus_tag="IVK51_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6432097.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5259..5402
                     /locus_tag="IVK51_002554"
                     /pseudo
     CDS             5259..5402
                     /locus_tag="IVK51_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            complement(5519..6100)
                     /locus_tag="IVK51_002555"
                     /pseudo
     CDS             complement(5519..6100)
                     /locus_tag="IVK51_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         2057 a          851 c         1089 g         2103 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat ttttttgaaa aatagtattg caaaataaag
       61 caatttcata gataataggt atcgtaaagt tatataactt tataatacct attagagtga
      121 gggataaaga aatgagacaa ctacacaaag aacagattga aacagcaatg gcagcgttaa
      181 aggaaaagac aattgatcat gtatatttta cagcatgtgg gggttcacaa gccgttttaa
      241 tggctggtca atatctattt gataaagaat taactgtacc ttgtcatgtt tatactgcta
      301 acgaatttaa ctatgctata ccaaaaggat tatctgaaaa ttctttagtt atcacttgtt
      361 cccattctgg cacaacacca gaaactgtag aagcaacaaa aaaagcaaga gaatttggag
      421 caatgactat ttgtttatct aatgaagaag gatctcctct atgggaagca gctgaatatc
      481 ctgttcacta tgattggggt aaagatgttg atgctagtga tttaaataaa ggaattcttt
      541 atggtttatt atttaatatt ttacttgttt tagatggctc taaaaaatgg gaaacttgct
      601 tgaatgaatt tgataagtta aatgatcttg ttattgctga aaagagcaaa tttgaaataa
      661 ctgctaaaga atggggtaaa aaccaaaaac gagataaaat tatttacaca attggtagcg
      721 gtattaatta cggagaaatt tactccacag cagcttgctg gttcatggaa atgcaatgga
      781 ttaattctaa tgctattcat tctggggaat ttttccatgg tccttttgaa atcactgatt
      841 atgatgtacc attcatctta gtgaaaagta ttggagatac aagattttta gatcaacggg
      901 tagaagactt cactaaaaaa ttcagtaata aattaatggt tttagaccaa gctgattttg
      961 acttagaatc tatagatagt gtagcgaaag aatatattgc agctgtactt agtggcgttg
     1021 ttattcgcca aatggtagaa gcgattgctt ttgaaagagg ccattcctta gatgttagac
     1081 gctatatgtg gcaaatgaaa tattaattaa taggaggttg caaaagatgg caaatcaatt
     1141 atttcacatt gtgattacaa cacattctac cctttgtgag ggttatttaa aggcaacaaa
     1201 attaatttta atgaatgacg ccacaggtat tagtgctgtt ccatttaaag aaaatactag
     1261 tccagatgat tttgaggaga gtatgaaaga aataattagt cagcatcatt tgcaacctct
     1321 aattatttta acggatttaa ttggaggaac acctaataat gtttctatta aacagttgaa
     1381 caacaataga attcaaatta ttgctgggat taatttacct cttttactgg agttattaat
     1441 ggcccagtca gctggtaaat ctatagaaga aatagatttt aaggaattac tgaataactc
     1501 tcaaaaaagt atggttcata ttaattcgct tttaaaagat gtcagttaag gtggcaattt
     1561 ctattctata aattttaaaa attgaaggag gaaatagaat gattaaatta gtgcgtgtag
     1621 accacagatt acttcatgga caagtaatat tttcttggac taaacagtat gatattaacc
     1681 atattattgt ggctgataac aaagttccag gagatccaat gtctgtgatg gctttgacaa
     1741 ttgctaaacc tgctgattgc aaattagaca ttgttaaaat tagtgatgtt aaagggatta
     1801 tggaaacatc cccaaatgat aattacatga ttttaattaa aggacctaag gaagcttttg
     1861 aattagttca aaatttacca gaaattaaag aaattaattt tggcggtgtg tctaagaaaa
     1921 gcgagtccaa acaatatgga aaggcagtct ttttaactcc tgaagaatta gaagccacaa
     1981 aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca agtcccatct tcaagtattg
     2041 aaaatgtaga ttttagtaag taagggggga aaataaatgt tattaaaagc cattttatta
     2101 ggtttagttg gtgtggtagc tgttattgac tccaggttaa ttggtcgaca aaatattggt
     2161 agaccactaa ttttgagtac acttgccgga cttgtgctag gtgacttgca tacagggatt
     2221 gttcttgggg catctttaga attaatttct atgggattcg tctcaattgg ggcggctgga
     2281 cctcctaata tgcaacttgg tagtattatt gccactgctt ttgcaatttt aactaatagt
     2341 agtacagaaa cagccttaac aatagctatc ccagtggctg tagctggtga atttatgagt
     2401 attatcatgc gcatgataat tgctcaattt ggacatgctg cagataaagc cattgaaaat
     2461 aataaattta gaaaagctca aattattcat atttattggt catttatttt taatgctttt
     2521 gtttacttta ttccaatctt tttaacagta tattttggtg ctgacgttgt ggcagattta
     2581 gtagataaaa ttccagaaat gattacagac gccttgactg tctctggaaa tatgctttcc
     2641 gcattagggt ttgcaatgct attaagcaca atgttgagta aaaaattata tccttacttt
     2701 atttttggat tctttattgt tgcctattca ggtctttcac taattggcgt gacaattttt
     2761 gcagctctga ttgcctttgt aatggatcaa gttaaatatg gaaaaagaga agaggcgcat
     2821 ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac aaaaaaagat cttcataaga
     2881 tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta tgaaagacaa atgcatatgg
     2941 gatttatgta tgggatggcg ccaattttaa ataaagttta taaaaatgat gaagagaaaa
     3001 aaagagaagc ttatactcgt catatggcat tttttaactg tacgcctcaa atgactccat
     3061 ttattatggg actttgtgct tctatggaag aacaaaatgc tcaagcagaa gaaggaaact
     3121 ttaatactga atctatctcc atgattaaaa ccagtttaat gggtcctttt gctggaattg
     3181 gtgattcatt tttccaaggt actttaagaa ttattacatt tgggattgga ttgtcttttg
     3241 ctaagcaagg tagtatttta ggaccgattt tagctgttgg cctttttgct ataccttcta
     3301 ttttagctgt tttttacggc ggtaaattag gatatgagtc tggcaataaa taccttacta
     3361 aattgtatca agaaggtctt atggatcgag taatgcaatt tgcttctatc attggtttgg
     3421 ctgtagtagg tggaatggtg gctagcatgg tgccaattgt caccccttta aaattcacta
     3481 cagggggaac agaacttgtt ttacaagact tacttgattc cattattcct ggattattac
     3541 cacttgtatg tactttggga atatataaat tgattaagaa aaatgttagt acaaatatgc
     3601 tcttattagg ttgtattttg gcagggtttg ttttaagtat aataggtatc ttataatgat
     3661 taaggctcta atttttgata tggacggagt tttaattgat agcgagatta tgtatcaaaa
     3721 gtgggtattt aattttttaa aacaagaaaa tttaagctat cctttgtatt cttatcaaaa
     3781 aaagattgga actacagcaa gtatttttga tgatatagaa aagtacaatg agggatgtaa
     3841 cagtttagaa ttgaagggaa aatttactaa atattggaaa tcgcaaaaga ttaattactt
     3901 ggatattttt cctaatatac taattgaaca gcttgaatgg ttaaaaaaga aaaaaataaa
     3961 aatggcaatt gcttcttcca gtccaatgga tactataaat gaagttgtta ccacttgtaa
     4021 aatagagtca tattttgatt gtttaattag cggaagagat ttgccagaaa gtaaacctaa
     4081 tcctactata ttccttaaag cttcagaaca gctcagaatt ccagttgaag aatgtctggt
     4141 gatagaagat tcttataatg gtataaaagc tggtaaaaga gcaaatatga aggttcttgc
     4201 aattaaagat aaacgttttt ctcaagatgt aagtctagca gatgatgtta tttacgatat
     4261 taaatatctg cgacaagaag tacaggtgaa atttggaata taaataagtt ttttgatgta
     4321 tttagagaat attagtaaaa ataaaaatca tgcccaaatc taatcaaaat tgattgcttt
     4381 gggtatgatt tcttttaata tcaatctatt actttaggat ctgaatcatt tttatgcacc
     4441 gtatctaaag tgatattttc tgtgacttca ataacataac taattctatc acaacgaact
     4501 gtggattgag aataatgaaa tggttgatta tttttatctt tgccaacctt ttctaaaata
     4561 aatagcggat ctcctactat acattgcaat aagtcggaaa gctcaggtgt tgcagataaa
     4621 ccgtttattt ccataataga gctcgtagct ttgacatcat agtcatcttc tagaagttta
     4681 tataatgaag tatattggct acctttttca aataaagttg gaaatttttc ttctgatgcg
     4741 aaaaaattat caattcccat aggggcaccg tcttcccaca ttaaacgttt aatattgtaa
     4801 actctatcag ttacattgat ttgtaattta ttagctaaaa actcatcagc ctttataatt
     4861 ttagaatgta aaacagtata tctaatttta tgtcctcggg tagaaagaga atcgctaaat
     4921 ccatgaactc cagctaattt taaactttct tttttttgag ttacaaaggt ccctttcccc
     4981 cttaatcgtt caactgttcc actttctgtt aaaagtttaa aagcttttcg aatggtaatc
     5041 ttacttactc cataaatttt acataattct tcttctgtag gtaattgctc tcctggaaga
     5101 tatttttttt cacgaatatc gttacgtata gaagcttcta cttgtagata taatggtcta
     5161 ttatcttgaa aattcatagt acactcccta aaaagttata cttcattata tcacaattta
     5221 ttaaaataaa agattaagat ataaatataa atgaactagt ctttgactaa ccaatgcccg
     5281 cttttgaaag aaaacaaatt cattctgttt taagtaaaaa tccttatgtt accactcatt
     5341 ctgaagggga tgagccttat cgctacttgg ttgtggaacc aagtgataag acaattttat
     5401 aaattcaata ttaattaaaa taaaaacaaa caaagtagat tattttactt tgtttgtttt
     5461 tttattggga gcgtcaataa ttttgtgtaa ataactcgtc cttctgcaaa ataatgggtt
     5521 actcagtaaa cattgaagct aatgtatccg tcacttgttg gaagccttta tggcttctat
     5581 ttaagaattt ttgattgtat gtatcaaaga tggatactag gaaacgctct agtgattctt
     5641 cattttgaaa ctgctctttt ctgcggctgt atttcttgat ttgtttcttg aaagattcga
     5701 ttaaattagt tgagtaaatg cttcggcgga taccaggcgg aaactcataa aaagtcaata
     5761 aatcttgatt ttttatcagt gactgcgtca ctttaggata agttttttgc tacttctcaa
     5821 tcatactccc taaaaaggta tttgcctctt cctttgagct agcttgataa acagccttaa
     5881 aatcatcata gatttctttt cgatctttga cacgtacttt atgagcaata ttacgggaaa
     5941 cgtgaacaca gcaatgttgg tatttagctt taggataaat ctggtgaatc gtatctttca
     6001 tgccttttaa gccatcggta ataaacagta agacatcatg aacccctctg gagtaaatgt
     6061 cctgtaacaa ctcattccaa acgtaggtcg attcagtcgg
//
LOCUS       DACCDF010000113         6065 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCDF010000113 DACCDF010000000
VERSION     DACCDF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.119"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            832..1566
                     /locus_tag="IVK51_002556"
     CDS             832..1566
                     /locus_tag="IVK51_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP6432098.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="IVK51_002557"
     CDS             1582..2751
                     /locus_tag="IVK51_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6432099.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="IVK51_002558"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="IVK51_002558"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP6432100.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="IVK51_002559"
     CDS             3841..4767
                     /locus_tag="IVK51_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP6432101.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="IVK51_002560"
     CDS             4786..6036
                     /locus_tag="IVK51_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP6432102.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DACCDF010000114         5925 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCDF010000114 DACCDF010000000
VERSION     DACCDF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.120"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(76..1170)
                     /locus_tag="IVK51_002561"
     CDS             complement(76..1170)
                     /locus_tag="IVK51_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6432103.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IVK51_002562"
     CDS             1359..2120
                     /locus_tag="IVK51_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6432104.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK51_002563"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IVK51_002563"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP6432105.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IVK51_002564"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IVK51_002564"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP6432106.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IVK51_002565"
     CDS             complement(3760..4773)
                     /locus_tag="IVK51_002565"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP6432107.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IVK51_002566"
     CDS             4938..5858
                     /locus_tag="IVK51_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP6432108.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACCDF010000115         5907 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCDF010000115 DACCDF010000000
VERSION     DACCDF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.121"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(95..667)
                     /locus_tag="IVK51_002567"
     CDS             complement(95..667)
                     /locus_tag="IVK51_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP6432109.1"
                     /translation="MTNKDQNKNYLEAIILELQNNSKRINSVELEQFSEKIVQAKHIF
                     LAGAGRSGIAMQAFTNRLMHLGFSVSLVGEISSPHSQPGDLLIICSGSGETGSLKNLA
                     AKANQSGVSIALVTMKKDSSIGQLADSVLVLPGITKDENQREEDTFSQPMGSSFEQLA
                     FLTFDSLVLSLMDKTGESSETMFARHADFE"
     gene            complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK51_002568"
     CDS             complement(674..1336)
                     /gene="rpe"
                     /locus_tag="IVK51_002568"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP6432110.1"
                     /translation="MTIAPSLLNAPIAEVKELFEILKEKNVDYLHIDVMDGCFVPDMA
                     FGPSVIKDIRPLTDVVFDVHLMIDTPEKIVTRFVEAGADIVTFHIEATKHAMKVIQLV
                     KEAGAKVGITFNPATPVSFIEPVLSLVDQVLVMTINPGTENKHFIQETVVKIEQLDVI
                     RKQNDYTYDIEVDGKIDNQTIKVCSKAGADIFVSGGFIFNGKNTPQEQIDKLNQSLEE
                     VK"
     gene            complement(1352..1867)
                     /locus_tag="IVK51_002569"
     CDS             complement(1352..1867)
                     /locus_tag="IVK51_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP6432111.1"
                     /translation="MRFHHDTLTIETVSGRPSYHDIRKELLDTVDTAGIENGMLVISS
                     PHTTCSLFFEETMHDTNFFGDDYLHVDINNVMETIIPKMTSENQYNSPGPKHIDFGTR
                     LSDPNYPAEKWVMLNTDAHLRSSIYGTNSMTLIVQEGKLLLGEMGRVYFVDWDQLRER
                     TRTINLMIMGE"
     gene            complement(1869..2153)
                     /locus_tag="IVK51_002570"
     CDS             complement(1869..2153)
                     /locus_tag="IVK51_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP6432112.1"
                     /translation="MKKLLVICGAGHATSTIAVAKLSKWLEEEGLTDQVKIYQSKIAD
                     ELNKIDEYDAVVSTTIVPDVIKAKVTNGLGLLTGIGTDAIYADVKERLGV"
     gene            complement(2187..3515)
                     /locus_tag="IVK51_002571"
     CDS             complement(2187..3515)
                     /locus_tag="IVK51_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP6432113.1"
                     /translation="MDIVLNAFEWFIGLGSNVFLPLIIFVIGILFGLKPGKAFVSGVT
                     VGIGSIGLGLVLSLLSDGLGTAIQQMGEQYGGGLNILDIGVGVGGPLAYSTSLGILMI
                     PISLILNFIFVVLGWTKTLNVDIWNFWFAIFLGMITQSVTGNFWFGLIGAIVAVILQW
                     FLADRTQKTVSEFFGYPGISITHMMGLSGVLFAKPVNWVFDRIPGFNKIDADAETLTK
                     RFGVLGDTVVIGLIIGIVVGIMAGYDVAGIGNLGIMTAAVMKIMPKMVAMFMEGLMPI
                     AEAAKEFTNKKLNGRQVNIGMDAALTVGHPTIMSTSLLLVPISLLLALILPGNKVLPF
                     GDLAFFAFAICLMIPYFKGNIVRSLVGCSLYVVTTLYMATWLAPIITDVFTLASFDVG
                     TAGLVTMLLCGLWPVGLFVLLASYFGVIGIGGLGVVLVGASIYMNKVKKA"
     gene            complement(3529..3984)
                     /locus_tag="IVK51_002572"
     CDS             complement(3529..3984)
                     /locus_tag="IVK51_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP6432114.1"
                     /translation="MYLDKELCLFQLEVKDQKELFQVMSEQLKNAGCVKDSFLEGITN
                     REQEFPTGLEVNQIGFAIPHTDSAHVNSSQICFASLKEPLVFSDMTDKSHEIPVRLVF
                     MLAMSQPHEQIDTLQNLVSLFQNEEKVNELLACTTKEAFIELVHSAGVY"
     gene            complement(3987..>5907)
                     /locus_tag="IVK51_002573"
     CDS             complement(3987..>5907)
                     /locus_tag="IVK51_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP6432115.1"
                     /translation="KLLSRQEDFKPASFFSDKLAVSTKTIYQDIELLEDYVVNYQVTI
                     ERLPSLGIKLIGDRKNVEHLISSVENYLEKDIYSPEKRRVEIIKEVLFGDKPVSLTHL
                     SQSFMVSKTSLYNDLRIINKIIGSGEVKLQSSAEGISFTGNEQAIQRAIKQLVFYYAN
                     DMESKTFFQVLNMLFDSKVISVIHDLLFNDYSELTEKVSDYYVRSLISTLVIQCNRLL
                     KGHHLTVEEEFLFNSIRYMETYIVANGLIEELKAKLNLPFYDADKEYLCRQLFAHRVT
                     NNLKTSNEEYAMSVHKLIERMSEIEKIDLTQNDRLYQSLLYHVPAMVLRLKKGIKIQN
                     PLLENIKSQYSELFSIVWYAMSILEEEFDVTLTDDEVSLILIHFQIAIEDQSKANNII
                     IICQYGISSAQLIYNKVRRFLPAKDNVEISTLDKFHLAEIGEVDLIISSLSFDAGGIP
                     LVRVSPLVSKTDYMNIMEAYTKYLIEEPSRFIQNELIPKAVKVPTISKFVQSSLVKTG
                     VQVESKEECLDLMISELEANSLVTEEFRASIFSREKMGNTNLESGVALPHANPSMVIE
                     SNIFIVALKNAINWGDRKVKLVIMVSLAEEDVSEIRGVYEELYQFIGQKEMVDRLLQV
                     HDSEKLLRIFQQKGE"
BASE COUNT         1919 a         1147 c          836 g         2005 t
ORIGIN      
        1 aattattaag ttatgtctaa tcaaataaaa aaaccattct catatttgct gaataattag
       61 catttagaga atggtttttt atctttaaaa tctactactc aaaatctgca tgtcgagcaa
      121 acatcgtttc tgagctttca cctgttttat ccattaaaga taacactaaa ctatcaaagg
      181 tgagaaatgc taattgttca aaacttgaac ccataggttg tgaaaaagta tcttcttctc
      241 gttgattttc atctttggta atacctggta atacaagaac agaatctgct aattgaccaa
      301 tcgacgagtc ttttttcatg gtaactaaag caatgcttac accactttga tttgctttgg
      361 ccgctaaatt ttttaaacta cctgtttcac ctgagccaga acagataatt aataaatctc
      421 caggttgcga atgtggtgaa ctaatttcac cgacaaggct gactgaaaaa cctaaatgca
      481 ttaagcgatt cgtaaatgct tgcatggcaa ttccagagcg tcctgcacca gctagaaaga
      541 tgtgtttagc ttgaacaatt ttctcactaa attgttctaa ctctacagaa ttaatacgtt
      601 ttgagttatt ttgtaattct aagataatcg cttctaaata gtttttattt tggtctttat
      661 tggtcatgat cttttacttc acttcctcta atgattgatt tagtttatca atttgttctt
      721 gcggggtatt tttcccattg aagatgaaac cgcctgatac aaaaatatcc gcccctgctt
      781 tagaacaaac tttaatcgtt tgattatcaa tcttgccatc tacttcaata tcatacgtat
      841 aatcattttg tttgcgtatt acatctaact gttcgatttt aactactgtc tcttgaataa
      901 aatgcttatt ctctgttcct ggattgattg tcattactaa tacttgatcg actaaagata
      961 atactggttc aataaaactg actggtgttg caggattaaa agtaatccct acttttgctc
     1021 ctgcttcctt gactaactga atgaccttca ttgcgtgttt ggtagcttca atgtggaagg
     1081 taacaatatc tgctcctgct tctacaaatc ttgtgacaat tttttctggt gtatcaatca
     1141 tcaaatgaac atcaaacaca acgtctgtta gtggacgaat atctttaata acacttggtc
     1201 caaaagccat atctggtaca aaacagccat ccattacatc aatatgcaaa taatcaacat
     1261 tcttttcttt taagatttca aataactctt taacttctgc gataggtgca tttaacaatg
     1321 atggagcaat tgtcattctc tattcccccg tttattctcc cataatcatg agattgattg
     1381 ttctagttcg ctctcttagt tgatcccaat cgacaaaata gacacggccc atttctccta
     1441 ataaaagttt accttcttga acaattaaag tcattgagtt ggttccataa atagatgaac
     1501 gtaaatgtgc atcggtattt agcatgaccc atttttctgc agggtaattg ggatcagata
     1561 atcttgttcc aaaatcaatg tgttttggcc ccggactgtt gtattgattt tcactcgtca
     1621 tttttggaat aatcgtttcc attacattgt tgatgtctac atgtaaataa tcatcaccaa
     1681 aaaagtttgt gtcatgcatc gtttcttcaa aaaagagtga gcaagttgta tgcgggctcg
     1741 aaattactaa cattccattc tcaatacctg ctgtatcaac tgtatctaaa agttcttttc
     1801 gaatatcatg atacgaagga cgccctgaaa ctgtttcaat agtcaaagtg tcatgatgaa
     1861 aacgcatatt aaacacctaa tctttctttt acatctgcat aaatggcatc tgtaccaata
     1921 cctgttaata agcccaagcc attcgttact ttagctttaa ttacatcagg gacaattgtt
     1981 gtacttacta cagcatcata ttcatcaatt ttatttagct catcagcaat tttagattga
     2041 taaattttca cttgatctgt taatccttct tcttccaacc attttgataa ttttgctact
     2101 gcaattgtag aagtagcatg acctgctcca caaataacta ataatttttt catattgatc
     2161 tcttctttct tattttaatt ttatgattaa gctttcttca ctttgttcat gtagatagaa
     2221 gcacctacta aaacaactcc taatccacct attccaatta caccaaagta agatgctaat
     2281 aaaacaaaca accctactgg ccataatcca cataacaaca tagttactaa tcctgctgtc
     2341 cccacatcga aacttgctaa tgtaaacaca tcagtgataa ttggtgccaa ccatgttgcc
     2401 atatataatg ttgtaacaac atacaacgaa catcctacta atgatctcac aatatttcct
     2461 ttaaaataag gaatcattaa acagatggcg aaagcaaaga atgctaaatc tccaaaaggt
     2521 aatactttat ttcctggaag aattagtgct agcaataatg agattggtac aagcaataga
     2581 gaagttgaca taatagttgg atgacccaca gttaacgctg catccattcc aatgtttact
     2641 tggcgaccat ttaacttttt attcgtaaat tctttcgccg cttctgcaat tggcattaaa
     2701 ccttccataa acattgctac cattttaggc ataatcttca tcactgccgc agtcataatt
     2761 cctaagtttc caattcctgc tacgtcatag cctgccataa tacccactac aataccgata
     2821 attaggccaa taacaactgt gtcacctaaa acaccgaaac gttttgttaa tgtttctgca
     2881 tctgcatcta ttttattaaa tccaggaata cgatcaaaaa cccaatttac tggtttggca
     2941 aatagtactc ctgataatcc catcatatgt gtgatagaga ttcctggata gccgaaaaat
     3001 tcgctaacag ttttttgtgt acgatctgct aagaaccatt gtaaaattac tgcgacaatc
     3061 gcgccaatta agccaaacca aaagtttccc gtaacagatt gagtaatcat acctaggaaa
     3121 attgcgaacc aaaagttcca aatatcaaca ttcaatgttt ttgtccaacc caaaaccaca
     3181 aatatgaagt taagaattaa tgaaattgga atcattaaaa ttcccaatga tgtagagtaa
     3241 gccaaaggtc caccaacacc tacgccaata tctaggatat tcaggccacc accgtattgt
     3301 tcccccattt gttgaattgc tgttcctaaa ccatctgata ataaacttaa aactaatcct
     3361 aaaccaatag aaccgatacc aacagtaacg ccggaaacaa atgctttacc aggttttaat
     3421 ccaaataata tcccgattac aaaaataatc agtggtaaaa agacgtttga ccctaatcca
     3481 ataaaccatt caaaggcatt taatacaata tccatctctc tattcctcct aataaactcc
     3541 tgcagaatgt actaactcaa taaatgcttc tttcgtggta catgcaagta attcatttac
     3601 tttttcttca ttttgaaata ggctaactaa gttttgtagt gtatcaattt gttcatgagg
     3661 ttgtgacatt gccaacataa agactaaacg tacaggtatt tcatgagatt tatctgtcat
     3721 atctgaaaat accagtggtt cctttaatga ggcaaaacag atttgagagc tatttacatg
     3781 tgcggaatct gtatgtggga tagcaaaccc aatttgattt acttctaatc cagttggaaa
     3841 ttcttgttca cggttggtga taccttctaa gaaactatct ttcacgcaac cagcgttttt
     3901 taattgttca gacattactt gaaacaattc tttttgatct ttcacttcta attgaaataa
     3961 acataattct ttatctagat acatttttat tctccttttt gttgaaaaat tcttagtaat
     4021 ttttcgctat catgaacttg taacaagcga tcgaccattt ctttttgccc aataaattgg
     4081 taaagttctt catatactcc tctaatctca gatacatctt cttcagctaa actaaccata
     4141 atgactaatt tgacttttcg atcgccccaa ttaatcgcat tttttaatgc tacaataaaa
     4201 atgttggatt caatgaccat tgatggattc gcatgcggta aagcaacacc actttctaaa
     4261 ttagtattcc ccattttctc tctagagaag atagaagcac gaaactcttc agtaactaat
     4321 gaattggctt ctagttcgga aatcatcaaa tccaaacatt cttctttgct ttccacctgt
     4381 actcctgttt ttactaaaga tgattgaaca aatttagata tggtgggaac tttaaccgct
     4441 ttaggaatca gttcattttg aataaatcgc gaaggttctt ctattaaata tttagtataa
     4501 gcttccataa tattcatata atctgtttta cttaccaatg gagataccct tactaagggt
     4561 ataccacctg catcaaagga aagagaggaa ataatcaaat caacttcccc aatttcagct
     4621 aaatgaaact tatctaaagt tgatatttct acattatctt ttgctggtaa aaaccgtcga
     4681 actttattgt aaattaattg cgcagaagaa ataccgtatt ggcaaataat gatgatatta
     4741 tttgctttgg actgatcttc aatagcaatc tgaaaatgaa tcaaaattaa ggaaacttca
     4801 tcgtccgtta aagtcacatc aaattcttct tcaaggatgg acatggcgta ccatactatg
     4861 ctaaagagtt ctgaatattg agatttgata ttttctaata acggattttg aattttaata
     4921 ccttttttta aacgaaggac cattgctggc acatgataca ataaggattg atataatcga
     4981 tcattttgag tcaaatcaat tttttcaatc tcactcattc tttcaatcaa tttatgaacg
     5041 ctcatagcgt actcttcatt gctggttttt aaattatttg tcacacgatg tgcaaatagt
     5101 tgacgacata gatattcttt atctgcgtca taaaatggaa gattcaattt tgccttcaat
     5161 tcttcaatta aaccatttgc cacaatgtac gtttccatat agcgaatgct attgaagagg
     5221 aattcttctt caacagttaa atgatgacct ttcagtaaac ggttacattg gataactaaa
     5281 gtagaaatca atgaccgaac atagtaatca gaaacctttt cagtcagttc actgtaatca
     5341 ttgaatagca aatcatgaat gacactaata acttttgaat caaacaacat atttaaaact
     5401 tgaaaaaatg tttttgattc catgtcattc gcataataga atacaagttg cttgattgct
     5461 cgttgaattg cttgttcatt accagtaaaa ctaattcctt ctgcagatga ctgcaactta
     5521 acttctccac tgccaataat tttattgata attctcaggt cattgtataa cgatgtttta
     5581 ctaaccataa aactttgaga caaatgagtt aaagaaacag gcttatcacc aaacaaaact
     5641 tctttaatga tctcgacacg tcttttttct ggagaataaa tatctttttc aagataattt
     5701 tctaccgatg aaatcaaatg ttcaacgttt ttcctatcac ctatgagttt gatacccaaa
     5761 gaaggtaatc gctcaattgt tacttggtaa ttaactacat aatcttccaa caattcaata
     5821 tcttgataaa tggtcttggt cgacactgcc aatttatcac taaaaaaaga agcgggttta
     5881 aagtcttctt gtcgacttaa taatttt
//
LOCUS       DACCDF010000116         5665 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCDF010000116 DACCDF010000000
VERSION     DACCDF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.122"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..194)
                     /locus_tag="IVK51_002574"
     CDS             complement(<1..194)
                     /locus_tag="IVK51_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432116.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFE"
     gene            complement(737..2887)
                     /locus_tag="IVK51_002575"
                     /pseudo
     CDS             complement(737..2887)
                     /locus_tag="IVK51_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3033..3608
                     /locus_tag="IVK51_002576"
     CDS             3033..3608
                     /locus_tag="IVK51_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432117.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3822..4517
                     /gene="vanR"
                     /locus_tag="IVK51_002577"
     CDS             3822..4517
                     /gene="vanR"
                     /locus_tag="IVK51_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6432118.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4495..5649
                     /locus_tag="IVK51_002578"
     CDS             4495..5649
                     /locus_tag="IVK51_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6432119.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1770 a         1092 c         1073 g         1730 t
ORIGIN      
        1 tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat aataaatatt ttcccttaat
       61 gtaccaacaa tggtaattcg gtcaattgac cattgcattt ttaagggggt gcacatagtt
      121 tgtgcattta accgcctgtt agaaatacgg ttaagttttt cccactcatt tacgattatg
      181 ccttttttac tcaaaaatag ctcctcctca acgaaaaaag agattcttgg aaagaatttc
      241 cagagttcac gtaactctaa aaatccacat ccaaaaatct ctagcttgaa gaaacctaaa
      301 aatattcgta taatgaactt gagttacgga actaggtgaa gtagtttccg taaaggtcga
      361 gagtgcgtca acactgctcg actatttttt tactcttttt tctcattttc tttgattata
      421 gcactcctct agaaaaaata aaaagacctc agggaatttc attccccgaa ccccttgagc
      481 ctcctcctgc cgaaatagaa tggcgctgcc agcatttatt tcgttcagct atcggctaca
      541 caatctaaca cgaccgttcc gctaccagtc gccttacagg ctccattttc gctcctctaa
      601 cgtgtttcac cgattgtgcc gataactaaa cgaaataaac gctaaaaccg ctcagaacgc
      661 aaaatatgcc attttagagc tattgtagaa aatatagttt gaaatgcaat tttcttcttt
      721 cagcttttta tttcccactt gtaaatcggt agatggtggc tccttttcca gttccataat
      781 gtggatttgc atctgcatgt agtgatgaaa cacctagctg cattctcata ccatctgacg
      841 aagatgttgt accgtcgccc caatagaaag gcaattgtaa tttatgatga aagtttacta
      901 atatggcttg ggctttattc atggcatctt catacatgcg ccattgagat acattggcta
      961 gttgcttata tgtaagtccg ggtgtggctt cggccatctt gctcaagcca atattcattc
     1021 ccattcctaa aagggcagcc atgataatga ttgtttcttc cttatctggt tttcgattat
     1081 tggaagcatg agtgaattgc tcatgaaatc ctgttatatg ggccacatcc atgagtaaat
     1141 cagttaattt tattcttggt agcatctgat aaaggcttgc actaaatttt tttgcttctt
     1201 ctggaacatc tttttctaag cgtgcaagtg atagctttcc tttttcaaga gaaaccccat
     1261 ctaacttatt ggaattggca gctaaccact ttaacctttc attaaagctg ctggttctct
     1321 ccgttatata atcttcgaat gataaactaa ctgataatct cgtattcccc ttcgattgat
     1381 tccatgtatc ttccgaaaac aaatattcct caaaatccct atattgtctg ctgccaacaa
     1441 tggaaacatc tcctgcccga acatgctccc gaagttctgt taaaacagcc atttcatagt
     1501 aatgacgatt aattgttgta ccatcatcct cgtataaatg tcttttccat cgttttgaaa
     1561 taaaatccac aggtgagtca tcaggcactt ttcgctttcc agattcgttc attcctcgga
     1621 taatctcaac agcttgtaaa agtggctcat ttgcctttgt agaatgaaat tccaatactc
     1681 ttaatagcgt tggcgtatat tttcttagtg aataaaaccg tttttgcagt aagtctaaat
     1741 aatcatagtc ggcaggacgt gcaagttcct gagcctcttc tactgaagag acaaaggtat
     1801 tccattcaat aaccgattct aaaaccttaa aaacgtctaa tttttcctct cttgctttaa
     1861 ttaatgcttg tccgatgttc gtaaagtgta taactttctc atttagcttt ttaccgtttt
     1921 gtttctggat ttcctcttga gccttacgac cttttgataa caaactaagt atttgcctat
     1981 catgaatttc aaacgcttta tccgttagct cctgagtaag ttgtaataaa tagatggtta
     2041 atatcgaata acgtttattt tcttgaaagt cacggaatgc atacggctcg tatcttgagc
     2101 ctaagcgaga cagctgcaac aggcggttac ggtgcaaatg actaatttgc actgtttcta
     2161 aatccattcc tcgtatgtat tcgagtcgtt ctattatttt tagaaaagtt tcgggtgaag
     2221 gatgacccgg tggctctttt aaccaaccca atatcgtttt attggattcg gatggatgct
     2281 gcgaggtaat aatcccttca agcttttctt tttgctcatt tgttagagat ttactaaccg
     2341 tattaaatag cttcttttca gccattgccc ttgcttccca caccattctt tcaagtgtag
     2401 tgatagcagg cagtataatt ttgttttttc ttagaaaatc tatgcattca tgcagtagat
     2461 gaatggcatc accattttcc aaagctaatt gatgaaggta cttaaatgtc attcgatatt
     2521 cactcagggt aaaagttaca aagtcgtatt cacttcgaat ttctttcaaa tgatcccaaa
     2581 gtgtattttc cctttgagga taatgatcaa gcgaggatgg actaacacca atctgtttcg
     2641 atatatattg tatgaccgaa tctgggatgc ttttgatatg agtgtatggc caaccgggat
     2701 accgaagaac agctaattga acagcaaatc ctaaacggtt ttcttccctc cttcgcttat
     2761 taactatttc taaatcccgt ttggaaaaag tgaagtaggt ccccagtatc cattcatctt
     2821 cagggatttg cataaaagcc tgtctctgtt ccggtgtaag caattctcta cctctcgcaa
     2881 ttttcattca gtatcattcc atttctgtat tttcaattta ttagttcaat tatatatcaa
     2941 tagagtgtac tctattgata caaatgtagt agactgataa aatcatagtt aagagcgtct
     3001 cataagactt gtctcaaaaa tgaggtgata ttttgcggaa aatcggttat attcgtgtca
     3061 gttcgactaa ccagaatcct tcaagacaat ttcagcagtt gaacgagatc ggaatggata
     3121 ttatatatga agagaaagtt tcaggagcaa caaaggatcg cgagcaactt caaaaagtgt
     3181 tagacgattt acaggaagat gacatcattt atgttacaga cttaactcga atcactcgta
     3241 gtacacaaga tctatttgaa ttaatcgata acatacgaga taaaaaggca agtttaaaat
     3301 cactaaaaga tacatggctt gatttatcag aagataatcc atacagccaa ttcttaatta
     3361 ctgtaatggc tggtgttaac caattagagc gagatcttat tcggatgaga caacgtgaag
     3421 ggattgaatt ggctaagaaa gaaggaaagt ttaaaggtcg attaaagaag tatcataaaa
     3481 atcacgcagg aatgaattat gcggtaaagc tatataaaga aggaaatatg actgtaaatc
     3541 aaatttgtga aattactaat gtatctaggg cttcattata caggaaatta tcagaagtga
     3601 ataattagcc attctgtatt ccgctaatgg gcaatatttt taaagaagaa aaggaaacta
     3661 taaaatatta acagcctcct agcgatgccg aaaagccctt tgataaaaaa agaatcatca
     3721 tcttaagaaa ttcttagtca tttattatgt aaatgcttat aaattcggcc ctataatctg
     3781 ataaattatt aagggcaaac ttatgtgaaa gggtgataac tatgagcgat aaaatactta
     3841 ttgtggatga tgaacatgaa attgccgatt tggttgaatt atacttaaaa aacgagaatt
     3901 atacggtttt caaatactat accgccaaag aagcattgga atgtatagac aagtctgaga
     3961 ttgaccttgc catattggac atcatgcttc ccggcacaag cggccttact atctgtcaaa
     4021 aaataaggga caagcacacc tatccgatta tcatgctgac cgggaaagat acagaggtag
     4081 ataaaattac agggttaaca atcggcgcgg atgattatat aacgaagccc tttcgcccac
     4141 tggagttaat tgctcgggta aaggcccagt tgcgccgata caaaaaattc agtggagtaa
     4201 aggagcagaa cgaaaatgtt atcgtccact ccggccttgt cattaatgtt aacacccatg
     4261 agtgttatct gaacgagaag cagttatccc ttactcccac cgagttttca atactgcgaa
     4321 tcctctgtga aaacaagggg aatgtggtta gctccgagct gctatttcat gagatatggg
     4381 gcgacgaata tttcagcaag agcaacaaca ccatcaccgt gcatatccgg catttgcgcg
     4441 aaaaaatgaa cgacaccatt gataatccga aatatataaa aacggtatgg ggggttggtt
     4501 ataaaattga aaaataaaaa aaacgactat tccaaactag aacgaaaact ttacatgtat
     4561 atcgttgcaa ttgttgtggt agcaattgta ttcgtgttgt atattcgttc aatgatccga
     4621 gggaaacttg gggattggat cttaagtatt ttggaaaaca aatatgactt aaatcacctg
     4681 gacgcgatga aattatatca atattccata cggaacaata tagatatctt tatttatgtg
     4741 gcgattgtca ttagtattct tattctatgt cgcgtcatgc tttcaaaatt cgcaaaatac
     4801 tttgacgaga taaataccgg cattgatgta cttattcaga acgaagataa acaaattgag
     4861 ctttctgcgg aaatggatgt tatggaacaa aagctcaaca cattaaaacg gactctggaa
     4921 aagcgagagc aggatgcaaa gctggccgaa caaagaaaaa atgacgttgt tatgtacttg
     4981 gcgcacgata ttaaaacgcc ccttacatcc attatcggtt atttgagcct gcttgacgag
     5041 gctccagaca tgccggtaga tcaaaaggca aagtatgtgc atatcacgtt ggacaaagcg
     5101 tatcgactcg aacagctaat cgacgagttt tttgagatta cacggtataa cctacaaacg
     5161 ataacgctaa caaaaacgca catagaccta tactatatgc tggtgcagat gaccgatgaa
     5221 ttttatcctc agctttccgc acatggaaaa caggcggtta ttcacgcccc cgaggatctg
     5281 accgtgtccg gcgaccctga taaactcgcg agagtcttta acaacatttt gaaaaacgcc
     5341 gctgcataca gtgaggataa cagcatcatt gacattaccg cgggcctctc cggggatgtg
     5401 gtgtcaatcg aattcaagaa cactggaagc atcccaaaag ataagctagc tgccatattt
     5461 gaaaagttct ataggctgga caatgctcgt tcttccgata cgggtggcgc gggacttgga
     5521 ttggcgattg caaaagaaat tattgttcag catggagggc agatttacgc ggaaagcaat
     5581 gataactata cgacgtttag ggtagagctt ccagcgatgc cagacttggt tgataaaagg
     5641 aggtcctaag agatgtatat aattt
//
LOCUS       DACCDF010000117         5120 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCDF010000117 DACCDF010000000
VERSION     DACCDF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.123"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(207..1169)
                     /locus_tag="IVK51_002579"
     CDS             complement(207..1169)
                     /locus_tag="IVK51_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP6432120.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IVK51_002580"
     CDS             complement(1171..2610)
                     /locus_tag="IVK51_002580"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP6432121.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IVK51_002581"
     CDS             2795..4741
                     /locus_tag="IVK51_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP6432122.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5120
                     /locus_tag="IVK51_002582"
     CDS             5005..>5120
                     /locus_tag="IVK51_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010051189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6432123.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEET"
BASE COUNT         1665 a          855 c          841 g         1759 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat
//
LOCUS       DACCDF010000118         5027 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCDF010000118 DACCDF010000000
VERSION     DACCDF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.124"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(45..554)
                     /locus_tag="IVK51_002583"
     CDS             complement(45..554)
                     /locus_tag="IVK51_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP6432124.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(666..1664)
                     /locus_tag="IVK51_002584"
     CDS             complement(666..1664)
                     /locus_tag="IVK51_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP6432125.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1865..2449
                     /locus_tag="IVK51_002585"
     CDS             1865..2449
                     /locus_tag="IVK51_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP6432126.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(2526..3701)
                     /locus_tag="IVK51_002586"
     CDS             complement(2526..3701)
                     /locus_tag="IVK51_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP6432127.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            3783..4163
                     /locus_tag="IVK51_002587"
     CDS             3783..4163
                     /locus_tag="IVK51_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP6432128.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            4237..4599
                     /locus_tag="IVK51_002588"
     CDS             4237..4599
                     /locus_tag="IVK51_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432129.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1628 a          871 c          936 g         1592 t
ORIGIN      
        1 aaagaactcg tctaaataga cgttttcttt ctttttttgt tgaattattg agtaaggcga
       61 cttctttttt ctggttcttt gtgtgctaat cgactggcag cagcagcggc gatcgctccg
      121 acgatatcat ctaagaaggt atggacatga acgccatcgt ggctgtttag ttcttttaaa
      181 ataccaggtt tcaccttgtc gatataacca taattcgtaa agccgatcgt accgtaaaca
      241 ttcacgattg acaaggctag gatctcatcg ataccgtaaa ggccttcgtc atcttcgata
      301 atatgttgca acggactcat caattttttc ttttctgcta atatatctaa ttggattcca
      361 gtgataatgg cgttatgtac ttcacgcttt gttagcacgc tgttgacact ttcagtacaa
      421 tccgccaaag tcaaatcaga aatatagtct ttttgtaaaa acatgacaag ttgtgcgata
      481 tcttctacag ttacgccacg ttccttcaat agttctcttg ccttttcttc taaagtctca
      541 gttttgacga ccatattatt gctccccctt tattttttct ttctctattt catgttaacg
      601 gaacatgaaa aaaatgcaag aaactgcttt cgggtttaac ttgaaatgca aagagatata
      661 aaaaatcaac taggacgaag atttttatcg tataagcttg tgctgtgacc aggctgagtt
      721 cgttcctttg gattaatata gtgtaaagcg ttgttcactg cagtaggtgc ttcaccgagc
      781 ccagtagcaa tcagtttcac ttttccttca taggtacaga tgtcaccagc tgcataaacg
      841 cctggaatag aagtagacat atctgatttt acagtaatgg catttctggt actatcaagt
      901 ccccaagaag aaagatgttc taaattagaa gagaatccat aattgacgat cagagaatca
      961 atcatcaaat caatcgtctc gtcacttttc acttttttta accggatatc tgtcagttcc
     1021 ccattattgc ctgataatcc atcgatgaga tagggagtaa gcaagttgac agatgaagat
     1081 ttcaagcgtg atacactatg ttcgtgcgca cgaaattccg ggcgacgatg aatcaagtaa
     1141 acttcgcttg caataggctc taacatcaac gtccaatcga tagcagaatc accacctcca
     1201 gcaatagcga ctttttttcc agcatacttc atcaaatcat tcacgaaata atccaatccg
     1261 tggttttcaa atgattcagc attatctaaa ttcaattttc ttggctggaa tgaaccattt
     1321 cctaaagcta aaatgactgc tttggaataa tggatgcctt tatttgtagt aatctcgata
     1381 acttcgtctt cacgagtaag cgagagaact tcttctttta aatggaatgt atggttaaaa
     1441 gtagttaatt gtttttctaa ttgatcgatt aattcacttg cttttattgc tggataccca
     1501 ggaatatcgt aaatatattt ttcgggatac aaagttgcta attgaccacc aagttgaggt
     1561 agactgtcaa tgatttttgt tttagcttgg cgcattcctg cgtaaaaagc ggcaaacatc
     1621 cctacaggac ctgcaccgat gattgtaatg tcatagattt ccataaaaat tcctcccttt
     1681 tcacactata gcagaattta attgagaatg cttctcaaaa atgtttgaat tcgaaatcga
     1741 aacggtaagt tatagaatgt atctagtgat gaaaaataaa agaaaacaaa tctagtattt
     1801 ttacgagagg ttaggtatta tgttattatt attatgaagt atgcaaatat taggaggaaa
     1861 caatatggca ttcccacaac tagatttaaa aagtgaaaaa ggaccaaaag ctgttatcaa
     1921 aacaaaccgt ggtgacatta ctgttcaatt attccctgaa ctagcaccga agacggttca
     1981 aaatttcatt gaactttcca aaaaaggtta ttatgatggc gtgatttttc accgcgttat
     2041 tcctgatttc atgatccaag gcggcgatcc aactggaaca ggaatgggtg gcgaaagtat
     2101 ttatggcgaa agttttgaag atgaattcag tcgtgaactg ttcaaccttc gcggagcttt
     2161 atctatggca aattcaggtc caaacacaaa cggaagccaa ttctttattg taaataacac
     2221 aaatgtgcca gctaatatgc ttggacaact agaaggtgct ggattcccat cagaaatcat
     2281 tgaagcgtat aaaggtggcg gaactccttg gctagatttc cgacatacag tctttggaca
     2341 tgtactagaa gggatggaca cagtagatga aatcgcaaat gtacaacgtg gtccacaaga
     2401 ccgacctgta catgatgtag tgattgaaac aattgaaatt tcagaatagt ttggtatggt
     2461 acggagtaat tatgaaaaag gacgaaacct agtaaggtct cgtcctttta ttttgaataa
     2521 atgatttaat cgattgtctc taaagcatac ttgattcgat taagtccttc cagtaaagtt
     2581 tcttctggac aagcaatatt caagcgcata tgcccatgac cggaaggacc aaacgtgatg
     2641 ccaggattta atacgacttt tcctttccga acaagagtgt tctccaattc ttggtcgtct
     2701 gaaatatagg cggtaaaatc taaccacatt aagtaagttc cttcagggtt cattattctc
     2761 actttcggaa gatactgttc aaaaaactga ttaactgttt gaacattttt ttctagataa
     2821 ggaatcagtt ggcttaacca ttctccaccc gtctcataag ccgcttgggt tccaatcagt
     2881 ccaaaagtgt tgatttcgtg ctgttggttc atcactaaat gtttttcaaa atcagctttc
     2941 atctctggat ttttgataaa taccattgaa tttttgattg cagctagatt gaaagtcttg
     3001 gttgcggaag taaaagcaac tagtagatca tctaaattag gatcaatcgt tagcatggaa
     3061 gtaaatgtat gatcgaataa agttaaatct tgatggatct cgtcactaaa cagaatcact
     3121 tgatgtttta aacacaattc gcttaactgt ttcaattctt ctttgctcca aacacgccct
     3181 ccaggattgt gaggattgca taaaatcatt gcttttacat tgttttcttc gatttttttc
     3241 tccatgtcag acaagtccat gatgaaatga ttattttttt ctagcaaaga gcttcgaacg
     3301 atgtgacgtt gatttgtttc tataatagag gaaaatggag gataaactgg gtcatgaatc
     3361 aaaatagaat cgcctggttt tgtaaaagtt tgtactgctg cagctaaact ggttaacaca
     3421 ccacttgtaa aaacgatatt ttctttcgtc aactccacag catggtgttg gcgttcccaa
     3481 tgaatgatag cctgatatag tttatcagac agagtggcat aaccaaagat tccatgtttg
     3541 atgtattcac taaacgcttt agctacccca ccgggagaca agaagtccat atctgctacc
     3601 cacaaaggaa gtaagtcatc catttgatat gtctcagcaa ttgcgtccca ttttacactt
     3661 tctgtatctt ttcttgaaat ctgtttatca aattcgatca tcttaagacc tccagtcatt
     3721 ttcgtcgctt ctttttttga gctatttaca gtataatgat aaatgcgaag agagggaagt
     3781 agatgactta taaaattgga caaataattg aaggaaaagt gacagggata cagccttatg
     3841 gcgcattcgt ttctcttgat aacgagacgc aaggacttat tcatgtttca gaagtacaat
     3901 ccggttatac gaaaaatatc cattcattgt taaaagtcgg acagccagta acagttcaga
     3961 tcatcgacat tgatgaatat tccaaaaaaa tcagtttatc tttaagaacg ttagaaaaaa
     4021 caaatccagc tgttccatat cgcagaaaaa ggtatttcac gaataaaaat cgtaagatcg
     4081 gatttaccac tattgcggag cagttaccta tttggataga agaagcatta tctgaactag
     4141 aacggaaaga aaataagaaa taaaaagtgt attttttctg aaaatgtgct agaattttta
     4201 tgtaagccat agaacaagtt gatcgaggtg gaaattttgg caagcaaaag agttgctggt
     4261 acaattatgt tgcatttgga ggatggtact aaaaaatttt tggtccattc aattgatgac
     4321 aagttggaat ttgcgttagc ggaactttct gaaggaaaga ctggtttagc gaatatactt
     4381 aatttcttaa aagaaatagt tcatatcgat gttagcaaga ttagtttgat ggaattgaca
     4441 aatacacata ttaaagagga aaatgttccg ttgtttgtct ttgaaactga acaaaagaat
     4501 caaagagaag aattaggcga aggatatatt tgggaagaac caggtcaaat gcgctctgtt
     4561 cttggaactt acgaagtaga gggagtgcct ttcttctaaa atacagatta agcgaacaaa
     4621 gaagtttgaa tttttttcaa acttcttttt ttattgtttg gtccaaaaaa tacaatgaaa
     4681 tgttctgcta aagatagttt tttagtgcat tttattttca aaatatgttg aaaacggttg
     4741 tataacgaat aataataatt gatcgaaaaa tcatttcaca aagtaatgct taatttatag
     4801 ttgaccaagg aagaaataca tgataaattt aacagcgttg attctgaagt ggatgttata
     4861 tcacatttct aaaaaattta aaaaatagtt attgacatgt tactaacgac atgataagat
     4921 aactgagtcg ttaaaaaaga accacttgcg aagcttgatt ctatcgaaaa aaacttcaaa
     4981 aaagttcttg acaaataaca aacgataagt tatgataaat gagttgc
//
LOCUS       DACCDF010000119         4791 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCDF010000119 DACCDF010000000
VERSION     DACCDF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.127"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(75..1073)
                     /locus_tag="IVK51_002589"
     CDS             complement(75..1073)
                     /locus_tag="IVK51_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6432130.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
     gene            complement(1148..1444)
                     /locus_tag="IVK51_002590"
     CDS             complement(1148..1444)
                     /locus_tag="IVK51_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6432131.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            complement(1437..1730)
                     /locus_tag="IVK51_002591"
     CDS             complement(1437..1730)
                     /locus_tag="IVK51_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432132.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1876..2427)
                     /locus_tag="IVK51_002592"
     CDS             complement(1876..2427)
                     /locus_tag="IVK51_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP6432133.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2601..2900)
                     /locus_tag="IVK51_002593"
     CDS             complement(2601..2900)
                     /locus_tag="IVK51_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP6432134.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            complement(2927..3193)
                     /locus_tag="IVK51_002594"
     CDS             complement(2927..3193)
                     /locus_tag="IVK51_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP6432135.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            3480..4223
                     /locus_tag="IVK51_002595"
     CDS             3480..4223
                     /locus_tag="IVK51_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432136.1"
                     /translation="MVVSKLPRNEDYLTNYDGYVVTTEHFDLANPVTKVDDIPDYEMY
                     SQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDD
                     EVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPIL
                     DQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFD
                     DLFALLKANGVTELAIYQKGKRIMVPLPV"
     gene            complement(4260..4757)
                     /locus_tag="IVK51_002596"
     CDS             complement(4260..4757)
                     /locus_tag="IVK51_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6432137.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
BASE COUNT         1428 a          912 c          696 g         1755 t
ORIGIN      
        1 taaaaaaact gattctaaga aaattaaata atttttctca aaatcagttt ttctttgata
       61 aataatattt tcaattattt aatcatttga aaattgttag tcattccaat acgttgagaa
      121 attcttccat ctgtataaac acgaaattgt tttccaccag ccatggtcgt gtttccatca
      181 tcatccgtta caatattaat tgaatagtag cttccatttt catcttgcat tagatctttc
      241 atatattcat ttgtgctaat taaattagta tttgatacat ttttatcagg agaatcatac
      301 aaccataaag ctttattatt tagcacagat tctttagcag cttcccaact tgaaattcct
      361 gaactacttg tagattgtgt cgttgtagtt gttgcttgtt gagtagattc ttctactact
      421 ggtgtttgag gagtttgtcc attcacaata tcactgaaac cttgttttaa ctgttgattt
      481 tgagtttcgt taaagtacag ataattattt tcattacctt gattttgttg cgttaaataa
      541 actttaggtt gtccattttg aaaaccgaaa aagtaaacat gctgccctaa atattctcca
      601 gtttctacat cagaataaac tgctactaaa ttgaaatctg catgaccagt tccatcttct
      661 gaccattgta cggtaactgg tgcttcattg attgccattt tccaatcgcc attaatcacg
      721 gcttgtggta aaggagtgcc atacagactt acattattct gaagattgta gcttttatat
      781 tcttgaccta atgtctttcc ccattgtgtg acaaaagttt ctaatttgga agctttattc
      841 gtatcccaca aagtattctt aacctcttca gaagaagatt cagtgattga acttgcactt
      901 gtttccacag attgcttcat ttctgttgta gtttgttcag ttgattgcgt tttacttttt
      961 ttagtctcaa ttgttgtatc agtttctata gtcgctttag tcctcgtatt ctcagatgta
     1021 caaccagtga ataaaattcc cattcctatt agtaataaca gccatttttt cattatattt
     1081 ccccctttca atttctccct cacgtactta ttctatcaga caattaattg atctaaatgg
     1141 taatctttta atcgattctt tcccaataat gtcgatagcc cgtatggcag cggtctcttc
     1201 cttgaggact ctcagatgga ttaaaactag tagaaatttc ccttccacat cctctacagc
     1261 gataagttct cattattttt gggctattag tattattttt cttagttaga gctatgagtc
     1321 caactatgat taccccaact gtcttaatta cttttccttt atctatattc ggatgcgcaa
     1381 gcatatattt atataaccaa tatccaccgt aaattaaacc acctaataca atcagcttag
     1441 ccatgtactc gtctcctttc tttttgttta attacttttt gaatccatct tgcagagaaa
     1501 ccataaactt ctgtcaattg acggatatct acagtttttt cctgttctat catgttttcg
     1561 attctttttc taactaactc acgatcgtac attttcattg gaaaagaaat ctgttgccct
     1621 cgaaaagcaa gataaaattg atagagcaat tctgaatcca gttccatttc ctcaaaaaag
     1681 tcattcatac ttttataaga gttactcaaa caagtttcat caatctccat ctacacaccc
     1741 cctgttattt tttacataat gaaaatagca tattactggg tacattgata attcatataa
     1801 catgtatcat cacatgtatt tttaaagtca aaaagcatgt aaaactactt ataagtaaca
     1861 tgagaaaata aaatgttatt tttcttgaaa taataattga ctatctgcca attgccattc
     1921 attacgaaca tgaatactta gtgggtggga taacttacct tctgcattta tcaatacttt
     1981 ggtcatcttc ttttgtttcg cactcaattg atcgtaaata gtctttgctt gttcataagc
     2041 cacttgactc gtcgctgtaa tcgaaccaca agcaaaaata atggtattga ttttcttttc
     2101 tgtaaaaaca gtcgttaata tttccaaaga ttgttcatca tttcctcgaa gaaatacccg
     2161 aggacttttc gcttttgtgt acgaaaaaag attcacagca ataaaaccag taaatcctaa
     2221 ttgtcggata ttcttttcga ttaataacat actcaaatca ttcgtaaatg gttctgtatc
     2281 tgttggacga atagtaatga ctactgctaa tttatcttcc gcttcttttc ctttgatcgc
     2341 ccattgtcgt ttgaaaacat agcgatgggt atgatgagga tctcgccaaa cttctgtttg
     2401 tgtggtcact aattcttttt caatcatctc tttttctcct atgctatttt ttgttcaagc
     2461 acttcaatta ccattgccgt tataagtgtc aatactagtt gaaaatattt catattcttt
     2521 ctcctttttt cttcagaatt tgatgaatcg cggtaaggat atcttctaaa catttcgcaa
     2581 tcattctcaa ataatcgatc ttattcatcc gttcctccaa tctgactcca aaccaatcgt
     2641 ccttctacca gtaacccacg aacaaattct cgctggctgg taatatctgt aatgaaataa
     2701 actacatagt cttttccatc ttctggtgcg caataaatat aaagatgtgt catcactaaa
     2761 cgtggtagat gaacatcttc aggaagcata cccatttcaa tttcctcttt ttccatcgta
     2821 acggattcat agccccactc ttcagaagca gtgaccagct gtgcacactt ttccagtact
     2881 tcgatagccg tttctaacaa gtcctttcgt tcctttcttt tagatactag tattcttctg
     2941 gtaataacat cgtttgatac tcacccgaat caatggccca tattttttga gacactgatt
     3001 tcaaacgaag cttaggtgaa atcagatact cttttctttc ttccggttct tcttgacgat
     3061 gaaccaactt ttgatttcct ttttccgtag aaaattcaaa aatttgaaga tagtctaaag
     3121 gaaattgctt ttgaacgttt tgatcgatta gattccaaca taaagcttgt aattccaccg
     3181 gcaaactgtc cattactccc tttgtgacat aacgttatcc tttattttga aacatttctt
     3241 ttccctcctt tgccataaaa atagcccaat cttctattaa agaagattgg gcttcatgga
     3301 tataatatat atttgtaaat aaagttaatt cagttataat tatcaaaact acacaattat
     3361 taaagttttt catatctctt tgtttagtaa gtttttctaa atcttaaccc gtggtgctag
     3421 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     3481 tagtagtctc aaaactccca agaaatgagg actatttaac aaactacgat ggttacgtcg
     3541 tgacaactga acattttgac ttagccaatc ctgttactaa agtcgatgat atccccgatt
     3601 atgaaatgta cagtcaaaca atcgatagct taaataagcg ttttggcaat cgtgttttaa
     3661 aaggcatcaa aatcggttac atcgcttctg aaaaggatcg gatcatcgac tatttagccg
     3721 ataaagatta cgatttaaaa ttactcagtg tgcatcataa tgggcaattt gattatttag
     3781 atgacgaagt caaagatatg gatcctgcta ttgtgattcc tcagtatttt gcacaacttt
     3841 cagaagcact agttgttata gaagcagatg tctttgctca ttttgactat ggttttcggg
     3901 tatttggtct atccgttgct gaatttaaac aatatgaagc gcagttttta ccgattctag
     3961 atcaagtaat caaaaataaa ttggcattcg aattaaatgc taagagcgct tacttgtatg
     4021 acaatttagc gttatatgaa tatgtcatcg atttatatct ttcacgtggt ggtaccttgt
     4081 ttagtgttgg atcagatggg cattaccttg aacatttccg tttccatttc gatgatcttt
     4141 ttgctctgtt aaaagcaaat ggtgtgacgg aattagcgat ttatcaaaaa ggaaagcgaa
     4201 ttatggttcc attaccagtc taaaaaaacg tcctcagcat caaaagtgag gacgtttttt
     4261 tagatttctt tttctaaaat cggacaaata cgatcttgtt ttcctaaagg aaaatgtttg
     4321 atcccgattt ttttaaatcc atgacgttca taaaaacgaa tacctgcatg atttttttca
     4381 taaacagtca aacgtaaata acgtttttgt ttcgttttag cataacgatt cgcttcatcg
     4441 agtaaccgtt gccctaaccc tttgttttga taatctttga gcagatagag ccgttgaatc
     4501 tctatagcat ctttttcatc attgactttc atataaccaa tgagtatttg atgatcctta
     4561 ataccaataa atttactggt tgactgtgcc aattctactg ccaatatttc agttgtcaat
     4621 tgtgtctgta aatatccttg catatcaagt ggatctgcgc catcgataaa cgattcataa
     4681 aaactgacaa cacttacaac ttgtaaagca tctagatcat cataatctaa atacgctagt
     4741 gtgatttgat tgttcatcta actcctcctt ttgacatagt atactataaa t
//
LOCUS       DACCDF010000120         4772 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCDF010000120 DACCDF010000000
VERSION     DACCDF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.128"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            544..1107
                     /locus_tag="IVK51_002597"
     CDS             544..1107
                     /locus_tag="IVK51_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432138.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IVK51_002598"
     CDS             complement(1386..1673)
                     /locus_tag="IVK51_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6432139.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IVK51_002599"
     CDS             complement(1663..1992)
                     /locus_tag="IVK51_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6432140.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IVK51_002600"
     CDS             2297..2626
                     /locus_tag="IVK51_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432141.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK51_002601"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IVK51_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6432142.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="IVK51_002602"
     CDS             complement(3551..4462)
                     /locus_tag="IVK51_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6432143.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1562 a          820 c          721 g         1669 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ct
//
LOCUS       DACCDF010000121         4726 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCDF010000121 DACCDF010000000
VERSION     DACCDF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.129"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            130..1275
                     /gene="tgt"
                     /locus_tag="IVK51_002603"
     CDS             130..1275
                     /gene="tgt"
                     /locus_tag="IVK51_002603"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP6432144.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1372..1752
                     /gene="yajC"
                     /locus_tag="IVK51_002604"
     CDS             1372..1752
                     /gene="yajC"
                     /locus_tag="IVK51_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP6432145.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            2057..4654
                     /gene="adhE"
                     /locus_tag="IVK51_002605"
                     /gene_synonym="adhC"
     CDS             2057..4654
                     /gene="adhE"
                     /locus_tag="IVK51_002605"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP6432146.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     AQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         1655 a          834 c         1010 g         1227 t
ORIGIN      
        1 ataaaaaaag gataaaaaaa agaatattaa ataaaataga aggaagccga gggaacttgc
       61 aaaaagcact tgaaacgatt atgatggtgg gagttgtcaa gatattttat atagaaagta
      121 gggaaatgaa tgacgcaacc tgccattcgc tatcgtttaa tcaagaaaga aaaacatact
      181 ggcgcgcgtt taggggaatt gatcacgcca cacggaactt tccccacacc aatgttcatg
      241 ccagtaggaa cattagctac agtaaaaaca atgtccccag aagagttgaa agaaatgggt
      301 gctggcgtta ttttaagtaa tacatatcat ttatggctac gccctggtga agacttagta
      361 gaagaagctg gcggactgca taaatttatg aactgggatc aaccgatttt aacagattca
      421 ggcggctttc aagttttttc attaagtgat atgcggaata tcgaagaaga aggcgtgcat
      481 ttcaaaaatc acttgaacgg ttcgcgtatg tttttatcac cagaaaaagc aatcaacatc
      541 caaaataaat tgggctctga tatcatgatg agttttgacg aatgtccacc attcgatgaa
      601 agttacgact acgtgaaaaa atcagtagaa cgtacgtctc gatgggcaga acgaggattg
      661 aaagctcatg ctaacccaga ccgtcaagga ttatttggaa tcatccaagg agcaggattt
      721 gaagatcttc gtcgtcaaag cgcgaaagat ctagtcggga tggatttccc tggttactca
      781 atcggcgggt tatctgtcgg ggaacctaaa tcagaaatga atcgtgtatt agaattcact
      841 acaccgttga tccccgaaaa caaaccacgt tacttgatgg gtgtcggtgc tgctgattca
      901 ttgatcgatg gtgttatccg tggagtagac atgtttgact gtgtcttgcc tactcgaatc
      961 gctcgaaatg gcacatgtat gacttctcaa ggtcgtctag tagtgaaaaa tgcgaagtat
     1021 gctcatgatt tccgtccaat tgatgagaaa tgtgattgtt atacatgtaa aaactataca
     1081 cgtgcctata tccgccattt gatcaaatgt gatgaaacat ttgggattcg tttgacttct
     1141 taccacaatt tatacttctt gttgaatcta atgaaacaag tacgtcaagc aatcatggat
     1201 gataatttgc ttgaatttag agaagccttt tttgaagaat acgggttcaa taaagaaaac
     1261 gcaaaaagct tctaaaggaa aatcttttaa aaattaagta aaaaactagt gttagcagca
     1321 tttttctgct aaagtgtttt ttgtagatac agaattagtg aggtgaaaaa aatgggaaca
     1381 ttaccaatgc ttgtgatgtt tatcgcgcta ttagcaatgt ggttttttat gtcacgttcg
     1441 caaaagaaac aacagcaaga acgtcaaaac ttgttagata gcatgaaacc aggtgattca
     1501 gtcgtaacga tcggcggatt acatggagtt gtttcagaaa tcgacaatga caaacggaca
     1561 gttgttttag actgcgaagg gatcttcctt gagtacgatc gtgcagcaat caaaacggtc
     1621 aaaccaggga ctgtcgtaac aaatgatgca acaactactg ttgaaacaac agaagaacca
     1681 gctgagacag ttgaagtaaa aactgaaaca acagaaacgc tggaagagac agaagagaag
     1741 aaagaaaaat aagtatcaaa gcttatttaa agacgaggta gggcagaaag atgtcctacc
     1801 tcatttttta ttcaaaaaag aaagagataa gagtatagga tatattgctc aatattgaat
     1861 aatcgggtgt gtcatataaa taataataaa taactttata tatttttcta tacaaacgcc
     1921 tataaataaa gcaactttat atttaaaaat aactgtgaaa aaaatcacaa aaaagtgttt
     1981 acatagaaaa ataaatattg tatgatatac atgtgaatga tgtaacaaat aaccatgacc
     2041 cattaggagg ataatgatgg ctaaagtaat ggaaaaagaa aaaacgaaga caattgacgt
     2101 acaagcaatg attgatgatt tggcagaaaa agcaaatgca gcattgaaag aaatggaaag
     2161 ctttgatcaa gaaaaagtgg accatatcgt gcatgaaatg gcaatggcag ctttagatca
     2221 acatatgcca ttagcgaaga tggctgtaga ggaaacaggc cgcggtgttt acgaagataa
     2281 agcaatcaaa aatatgtatg cgtccgaata tatttggcat aatatcaaac atgataaaac
     2341 agttggtgta atcaatgaag atgcccaaaa aggattgatt gaaattgctg aaccagtcgg
     2401 tgtggtttgc ggagtaactc caacaacgaa tccgacttcc acaacgattt tcaaatcaat
     2461 gatcgcgttg aaaacaagaa atccaatcgt ttttgctttt catccaagtg cccaaaaatc
     2521 ttctgcagaa gcagcacgag tagtacgtga tgctgctatc gctgccggag ctcctgaaaa
     2581 ttgtatccaa tggatcgaac atccttcgat cgaagcgact tctatgctaa tgaaccatcc
     2641 aggtatcgcg atcgtcttag cgacaggtgg agctggaatg gtcaagtctg cttactctac
     2701 aggaaaacca gctttaggtg taggtccagg taacgtacca gcttatattg aaaaaacagc
     2761 aaaaattaaa cgtgcagtga acgatttgat cgtttcgaaa acatttgata atggaatgat
     2821 ttgtgcttct gaacaagctg tgatcgtgga taaagaaatc tatgcagcag taaaagcaga
     2881 attccaagca caccaagttt atatcgtaaa accagatgaa ttgcaaaaat tagaagatgc
     2941 agtgatgaac gaaggaaaat atgcggtcaa tccatcaatc gttggacatt ctgcgatgga
     3001 aatcgcaaaa ttggcaggaa tcagtgtacc aaaaggaacg aaaatgctga ttgctgaatt
     3061 ggaaggcgta ggcccagatt atccgctatc aagagaaaaa ctttctccag ttcttgccat
     3121 gatcaaagct aacaacacgg accatgcgtt tgatttgtgt gaaggtatgt tgaatcttgg
     3181 aggattagga catacagcag ttatccattc agaaaacgaa gagttgcatg tcaaattcgg
     3241 tcttcgaatg aaagcttgtc gtattttagt gaatacacca tctgcagaag gcggcatcgg
     3301 tgatatctat aacgaaatga ttccgtcatt gactttggga tgcggttctt atggtaagaa
     3361 ctctgtttct cgtaacgtct ctgctgtaaa cttaatcaat gtcaaaactg tagcgaaacg
     3421 gagaaataac atgcaatggt tcaaattacc gccaaaaatc ttctttgaaa agaactctct
     3481 attgtactta gagaaaatgg aaaacgttga acgtgtcatg atcgtttgtg accctggtat
     3541 ggtccaattc ggttactgcg atacagttcg tgaagtattg gcacgacgta aaaatgatgt
     3601 gaagatcgaa gtcttctcag atgttgaacc gaacccatcg acaaataccg tatacgcagg
     3661 aacgaaactg atggcagact tcaagccaga tacagtaatc gctcttggag gtggttctgc
     3721 aatggacgca gctaagggaa tgtggatgtt ctatgaacac ccagacacat ctttctttgg
     3781 tgcaaaacaa aaattcttgg atatccgtaa acggacatat aagatcgaca aaccagaaaa
     3841 aacacaattc gtatgtatcc cgacaacatc agggactggt tctgaagtaa cgccatttgc
     3901 ggtcatcact gacagtgaaa cacatgtgaa atatccgtta gcagattatg cattgacgcc
     3961 agatgtcgca atcgtcgatc ctcaattcgt catgtcagtt ccagcatctg tcacagcaga
     4021 taccgggatg gatgtgttaa cacatgcaat cgaatcctat gtttcagtca tggcttcaga
     4081 ttatacacgt ggattgagcc tacaagcaat caagttagtc tttgactatt tagaaaaatc
     4141 agtaaaaaca cctgatatgg aatcacgtga aaaaatgcac aacgcttcta caatggctgg
     4201 tatggcgttt gctaacgcat tcctaggtat ttgtcactca gtcgcacata aaatcggcgg
     4261 ggaatacggg atccctcatg gacggacaaa tgcgattctt ttaccgcata ttatccgcta
     4321 taatgcaaaa gatccacaaa aacatgcgat gttccctaaa tatgactatt tccgtgcaga
     4381 tacagattac gcagatattg caaaattctt aggtctgaaa ggcgaaacaa cagaggaatt
     4441 agtcgaagct ttagcaacgg ctgtttatga attaggaaaa tctgtcggca tcaacatgag
     4501 tctaaaagct caaggagtca cacaagaaac actagataca accgtagacc gtatggctga
     4561 attagcttac gaagaccaat gtacgacagc taatccgaaa gaaccattga tctctgaatt
     4621 gaaacagatc atcattgatg cttacaacgg ataagcaaaa gaaagcgtag aaaaaagata
     4681 agaaaataga tgaaaaagaa cttggaaaac gttgtttctc caagtt
//
LOCUS       DACCDF010000122         4571 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCDF010000122 DACCDF010000000
VERSION     DACCDF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.130"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(338..1291)
                     /locus_tag="IVK51_002606"
     CDS             complement(338..1291)
                     /locus_tag="IVK51_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP6432147.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1314..2069)
                     /locus_tag="IVK51_002607"
     CDS             complement(1314..2069)
                     /locus_tag="IVK51_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6432148.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2066..3025)
                     /locus_tag="IVK51_002608"
     CDS             complement(2066..3025)
                     /locus_tag="IVK51_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6432149.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3018..3968)
                     /locus_tag="IVK51_002609"
     CDS             complement(3018..3968)
                     /locus_tag="IVK51_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP6432150.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4118..4570
                     /locus_tag="IVK51_002610"
     CDS             4118..4570
                     /locus_tag="IVK51_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP6432151.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
BASE COUNT         1490 a          962 c          775 g         1344 t
ORIGIN      
        1 agaagagatc ggtacaaaat aaaaatatct aaatttaagt tttatctaag tttcgctttg
       61 tacctttgat tcaaatcaaa ggaggcgatc ttgatgctga aaaaatggat cacactattt
      121 tttgccactc cccattctag aatcaacccg tatgatgtag agaatgaatt cacgttgaaa
      181 aaagatttcc caaataaata aaaaccatcc gtaaaatcat caatacgatt ttacggatgg
      241 tttttgataa cttctttgag gataggctct tgctatttaa gaatgtaaga aacattattg
      301 ttacatagta tgagagttta atcctctgtt atttatgcta tttaaatgct tgattcacat
      361 cttcgatcat cagtttcatt gattctaaac cgcccccgct taaataccag acatctggtt
      421 caagggagat gatctgctga tttttaccag cattcgtttg tgctaccagt tcattggcag
      481 aaatatcatc tttgctgtcg tctccaccaa tagcttttgt tctgtctact acaaataaga
      541 tatccggatt tttttccagc acatattcat aagaaacact ttgaccgtga gtggatgctt
      601 cgatttgatc atctgcttgt tcaaagccaa ataaatcatg gatgaaaccg aatctagaac
      661 cagatccgta ggctgataat tgcccttcat tcacaagtgt gactagtgct tttttgtcag
      721 tagcttctgc tttctttttc gtctgatcga ttgcactaga taattcctct aattgagttt
      781 tagcttcttc ttgcttacca aagatctccc ctaaagcagt gacattttgc tggaaagatt
      841 cccatggatt tttcgcatct aaagaaagga aaatggtcgg agcaatttga cttagatctt
      901 tttgaaaatc tctttgccgg ccagaaatga tgatcaggtc tggctgaagc tgattgattt
      961 tttctaagtc tggttctttg atcccacctg ctgattccac ttttttatag gatgaaagat
     1021 actcaggaag gctgcttgta gctgctccaa ctaccttatc tccaacacca agagcatcca
     1081 tcgtatctaa agaaccattg tcgaatacga cgactttttc aggattcttt ggcacctcga
     1141 tttgatcgcc attactgtct tttatcgtta atgccgtttc agcttgcgta ctgcttttct
     1201 ttgtatctgc ctgactggtg ttgtcactat ttccacatgc tgctaaacca aatgatgcta
     1261 gaatcaagat acctactact aattttttca tctcttctct cctaatgtta gttttagaaa
     1321 taaagacaga atctgcgacc ttcaatctca caaattcgta tgtccatttc gtacaattcg
     1381 tccagtacat ctttttttat catttcttgg gtcgttcctt gcgtgaataa ctgcccatct
     1441 ttcattgcaa taatttcgtc tgcataactt gcagcaaagt tgatatcatg aagaacaata
     1501 acaattgttt tcccgatttc ttgtactaat ttttttaaga tctgcatgat ttgaaccgca
     1561 aaattcatat ctagattgtt caaaggttca tccagtaaaa tataatccgt gtcttgcgct
     1621 agcaccattg caatatacgc acgttggagt tgaccgcctg aaagagtgtc aatcgattga
     1681 tccgctattt cttctaaccc aaggtgttcc agtgattcct gaacgatttc ctcgtctttt
     1741 gtcgtcaatt ttcctttcgt ataaggaaat cgtccaaatt ctactaattc tttcacggtg
     1801 atttggacat ttatttcatt cgattgtcgt aggatcgata attttttggc taattcattg
     1861 gatttccaag cctttacttc gttatggtct aaatagattt cacctgtatc ttttggtatc
     1921 aaacgactga tcatcgacaa caccgttgac tttccagcac catttggacc gataaaagca
     1981 gtcaacttct tttcaggaat cgttaactgt acatctttaa ccactgattt tccatggtac
     2041 agtttattga ccttttgtaa atcaatcata ggatactctt cctttcgcga atcaattttg
     2101 tgacaaaata aattcctccg acaaattcaa cgacgacact cactgttgta ttccagccga
     2161 aaatctgctc tactgcgagc tgtcctcctg tcagaaacag catagctacc agaaagcttc
     2221 cgataaatag ctgaccatga cgataagtag aaaagacacg ataactgata tttgcgacaa
     2281 taaaccctaa aaaagtaact ggacccacaa gagccgttga tgtccctgtc aaaagactga
     2341 cacaacataa aatcaaaaat tcaaaacgat ctgtgtctat cccgaggttt actgcttgtt
     2401 catttccgag atggaaaacg tctagcacat gtcgatatcg aaataacagg taccagcagc
     2461 cgataatgat caaagaagca ggcaaaagat actgactctg gatattgcca aagcttgcaa
     2521 ataatctgcc ttgaagcaaa tcatattcat tcggatccat gatgacttgt aaaaaggtac
     2581 tgacactacc aaaaagcgta cctaaaatca tccctgacat cagtaataaa aaaagattcg
     2641 ctctcatttt tttcaaaaga aagaaaaata acaatagact cagcccgacc atcagcccaa
     2701 cgttcaataa gaaaaaaact gtacctgtct gctgaagcat cgtcactcct ccgataaaga
     2761 agaaaaggac cgtttggaca aggacatata acgactcaag tcccaatact gaaggagtca
     2821 aaaactggtt atgcgtaatt gtctgaaagc tgattgtggc actgctgaca gctagcgaga
     2881 caaggataaa tgcaaaaatt ttcttactcc tcaatgccca tgcaaaatgc cagtttccat
     2941 atgtatggat cgtcaagtac attcctgtaa caaataaaac aagaacaagc ataccaataa
     3001 aaatagatgg ctttttttta agcatatcgt cgccccctta tcagaagata aaggaagata
     3061 gccccaccga tgattccaac gatcacactg actggtattt cataaggaaa aatcaacacg
     3121 cgagcgacaa gatcgcaaaa tactaagaac aaactacctg ctaaagcagt tacgccaatt
     3181 gttttgtgca cttgatctcc atatattcta gagacaagat tcggaatgat cactccgata
     3241 aaaggaatgc ttcctaccat gatcaacatg actgcactag ccaatgatac taagccaatg
     3301 cctaaaaact gcatcttctg gtaatctatt cccaaattta ctgcaaacga ttcgcccaga
     3361 ccaatgatcg taaatcggta agcaaatagc caaaccaaga gcaacaatgg aacaacagca
     3421 tacaataact cataatttcc tcgtatcaca gtcgcaaaat ttccttgaag ccatgaagtc
     3481 atattttgta ccaactggaa ttgataagca aaaaaagtag caactgaacc aatgatattc
     3541 cctaacatga ctccaatcaa cggaagcatc aattgatttt tctgggggaa caatcgcgta
     3601 agggataaaa aaagcaatgt tcctgcaaaa gcaaagcaaa atgctgtcaa ggatcggaca
     3661 atcaaggatg caccaggtag gaaaatcata gccactaaaa tacctaaacg cgcactatcc
     3721 atcgtgcccg cagtcgttgg tgaaacaaat ttattttggg tcaaatgttg catgatcaac
     3781 ccacaaatac tactggtcgc tcccacaagg atcaaacaaa tcgttcgtgg caaacgagtc
     3841 actagcaaga tttgtaagct ttgcccatct aactggagca gttctgacaa ttgtacattc
     3901 gatactccga tgaacaatga aatactactt aaaataacta ataatgctac tagtcgtccg
     3961 tagcgcatcc aatccacctt tctaattgag aatgattttc attcttattt taagtgaatc
     4021 gttgaacaaa agcaaccgct ttttcatatg ttttaggtat tattgggaat atgctataat
     4081 cgtaagaaag acaaagagaa gaaggagggt attttttatg gcacaagatg atgtaaaaag
     4141 ccatttggat aagggacttt atggtacacc tttagtgaac cctgaagaac agcataaata
     4201 tttagggaca ttcagggaac gctgctatct tagtatgact gttgctgaaa tgatccaacc
     4261 aaaaaacaaa gaaaactttt taaaagaact caaaaaacat ccagatgctt ccatactctt
     4321 gaatggggct ctaccccttt ctgtccaaaa ccaatatatc cagctagcaa cgaaaaacaa
     4381 tagccgattt actgtagtca acgattacgt cactgcaaat cctgaggaat tcggattgct
     4441 cttaacagca aaagaagcag taaatgaacc tgtgattgat atcgaaaaaa aatacccaga
     4501 agaaaactct tcgccagttg aaaaagaaaa accaaaaaag aaaaaaagct tctgggatcg
     4561 tttattttaa a
//
LOCUS       DACCDF010000123         4427 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCDF010000123 DACCDF010000000
VERSION     DACCDF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.131"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(543..2048)
                     /locus_tag="IVK51_002611"
     CDS             complement(543..2048)
                     /locus_tag="IVK51_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP6432152.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVK51_002612"
     CDS             complement(2045..2731)
                     /locus_tag="IVK51_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP6432153.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVK51_002613"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVK51_002613"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP6432154.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1237 a          918 c          729 g         1543 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaa
//
LOCUS       DACCDF010000124         4401 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCDF010000124 DACCDF010000000
VERSION     DACCDF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.132"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(99..1619)
                     /locus_tag="IVK51_002614"
     CDS             complement(99..1619)
                     /locus_tag="IVK51_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6432155.1"
                     /translation="MTKMESELLVQIKQLTQLLEIQQQENTLLREQIAEMNRRLFGRK
                     KETPPVDGQIDLLDDSTFNEPEHTGQESQEPITVSSFKRRKRKGLKALSLEGLPEVPI
                     HYELKGADCLCEDCGHTLHDIGATRLREEPLYVPAHIEKQVIYQHAYHCRECEALGET
                     KIKKAPVPKPLINNSLGSSSIVTQSIWEKFVKKVPAYRQEKEWHALGFPLDRKKITNW
                     HIKVSEMVLEPIYNCLHQELSNQEVLHADETSYRVLNTHKEKTFYWLYASGKSEEKQV
                     VLYEHANSRSVDEPKSFLESFSGFLHTDGYAAYGKLPVNNVYCWAHVRRKFFEALGKQ
                     PSPKSHAKIGLDFCDKMFRIERTFNKLSAEERFVARKETLQPVMTEFFHWCESFAYLP
                     KSQLGKAVQYAVNHKDGLQIVLLDGRLELSNNRAERAIKELVIGRKNWLFSKSLKGAR
                     SNGIILSIIQTAVANGLNIRKYLNHLFTEIPNLSSMTPEALRAYLPWNQQIQEICK"
     gene            complement(1715..1840)
                     /locus_tag="IVK51_002615"
     CDS             complement(1715..1840)
                     /locus_tag="IVK51_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432156.1"
                     /translation="MYKRFESGYLQWPRSRKEVLLTGLSIEEKCTLKETKKGAIY"
     gene            complement(1862..2047)
                     /locus_tag="IVK51_002616"
     CDS             complement(1862..2047)
                     /locus_tag="IVK51_002616"
                     /inference="COORDINATES: protein motif:HMM:NF017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAP6432157.1"
                     /translation="MPMLIDATALKHIFIVCGKTDLRKGIDGLAAFVIEEYDLDVYDQ
                     ALFLFCGTKKDRFKALY"
     gene            complement(2031..2213)
                     /locus_tag="IVK51_002617"
     CDS             complement(2031..2213)
                     /locus_tag="IVK51_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432158.1"
                     /translation="MNKKDIVPVQFSSAAQKSQIDTKPKLACRIKSEDKEIFIYNGMD
                     KYLLYTLLKELNANAH"
     gene            2454..2672
                     /locus_tag="IVK51_002618"
                     /pseudo
     CDS             2454..2672
                     /locus_tag="IVK51_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            3167..3766
                     /locus_tag="IVK51_002619"
     CDS             3167..3766
                     /locus_tag="IVK51_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6432159.1"
                     /translation="MKTVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLADVLEISRGRVVVKDRLAMKEQKTAKHNSVYISNKLRKTILEYVQQEFPDQ
                     LLQQDFDGYLFPSRKGTNEPLNRQSLWRIIHDAGTAIGLKDIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            3830..>4401
                     /locus_tag="IVK51_002620"
     CDS             3830..>4401
                     /locus_tag="IVK51_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432160.1"
                     /translation="MSTLYIIGNGFDLSHGLKTRYTDFSKWVEKSHSEIFDKIDTMIN
                     ENNEVRSTADDKIGWFNFESSLHELIQSPVFEDYVEESKDYLIGYGHEDWSDSAHHDF
                     QNMVYDYVVSLEELQQLFKEWIRKEIQPTIEHKIPKYQFSSDVLFFTFNYTDVLEILY
                     KQPKENIYHIHGSYDDIIIGHSENFDNFIG"
BASE COUNT         1403 a          799 c          785 g         1414 t
ORIGIN      
        1 ataattcagt agttttgagc gtaagcgtgt catagaatgg tacctaaaca caaatagccc
       61 gatttgatac actcaaatca ggttatttgt ggcttatatt atttacagat ttcttgtatc
      121 tgctgattcc atggcaaata agcgcgtagc gcttcaggtg tcatcgaaga cagattggga
      181 atctctgtaa aaagatgatt taaatatttg cggatgttta acccattcgc aacagctgtc
      241 tggataatac tcaatataat tccatttgat ctagcgcctt ttaagctttt agaaaatagc
      301 cagttttttc gaccgatcac taactctttg atggcacgct ctgctcggtt atttgaaagt
      361 tccaagcgtc catcaagcaa cacaatctga agtccgtctt tatgattgac tgcgtattga
      421 accgcctttc caagctgcga ttttggcaaa taagcaaaac tctcacacca atgaaaaaat
      481 tcagtcatca caggctgcaa cgtttccttc ctcgctacaa acctttcttc tgcgctaagc
      541 ttattaaagg tccgttcgat tcgaaacatc ttgtcacaga agtctaatcc tattttggca
      601 tgactttttg gggacggttg tttacccaat gcttcaaaaa actttctgcg aacatgcgcc
      661 caacaataaa cattgtttac cggcagcttt ccataggccg cgtaaccatc agtatgcaag
      721 aaaccgctaa atgattctaa aaaacttttt ggctcatcaa cggaacgcga atttgcgtgc
      781 tcgtagagca caacttgttt ttcttcgctt ttacctgaag catataacca gtaaaaggtc
      841 ttttctttat gagtattcaa tacccgatag cttgtctcat ccgcgtgtaa gacctcctga
      901 ttgcttaatt cttgatgaag gcaattgtag attggctcta aaaccatttc acttaccttg
      961 atatgccaat tagtgatttt tttacgatca agagggaaac caagtgcatg ccattctttt
     1021 tcttgtcgat atgccggcac ctttttaaca aatttttccc aaatactctg tgtcacaatt
     1081 gaagaggaac ctagactatt attgatcaac ggtttaggaa caggcgcttt tttgattttt
     1141 gtttctccta atgcttcaca ttctctgcaa tgataggcat gttggtagat gacttgtttt
     1201 tctatatggg caggaacata caatggctct tctctaagcc ttgtcgcacc gatatcatgt
     1261 aaagtatgac cacagtcttc acacaaacaa tcggcacctt ttaattcata atgaatagga
     1321 acttcaggca gaccttctaa tgaaagtgct ttgagtccct tgcgttttct gcgtttaaaa
     1381 gaagataccg taatgggttc ctggctttct tgtccagtgt gctctggttc attaaaagtt
     1441 gagtcgtcta ataaatcgat ttgcccatct acgggtggtg tttctttttt tcgtccaaac
     1501 aagcgtcgat tcatttcagc aatttgttct ctaagaagag tattttcctg ttgctggatt
     1561 tcaagcaact gtgttaattg cttgatttgt acaagaagtt cagattccat ttttgtcatt
     1621 tttacaccac cattctagcg attttcttac atctattata ccattttatg acaacgaaaa
     1681 acagacaaaa tccttgattt aacaaggttt tcgtctaata tatcgcacct tttttcgttt
     1741 cttttagcgt acatttttct tcgattgaaa ggccagtcag taagacttct tttctggaac
     1801 gcggccattg aaggtaccca ctttcaaatc ttttgtacat taatacaaat ccattttgat
     1861 cctagtaaag agccttgaag cgatctttct tcgtaccgca aaataagaaa agcgcttgat
     1921 cgtaaacatc aaggtcatat tcttctataa cgaaagctgc tagtccatca attccttttc
     1981 gaagatctgt ttttccacaa acgataaaaa tatgtttcaa tgcagttgca tcaatgagca
     2041 ttggcattca actcttttaa caaggtatac aaaagatatt tatccattcc attataaata
     2101 aatatttcct tatcttccga tttgattctg caagctagtt ttggcttggt gtcaatttgc
     2161 gatttttgag ccgcacttga aaattgaact ggtacaatat cttttttatt cattcgtttt
     2221 tctccttcta tatttgatag tttaattata gaagaaagat aatcggcgta cgagatacta
     2281 tcctatgaca cgcttacatt taatttaata ttttgcctca aattagccac actttttata
     2341 gaactaagag gaaaaatgaa gttttcttcc tattaatata gttgtttgaa atcttaaata
     2401 tagcgtttta cgatgtaatg ggaagtatgg tgaataaatc aactatgttc gtacgaagca
     2461 tttactcaac taatttaatc gaatctttca ataaacaaat caagaaatac agccaccgca
     2521 aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt agtctcgtct tttgatactt
     2581 acaaccaaaa attcctaggt cgcagtcata aaggtttcca acaggccgaa ggcgagctcg
     2641 aacaaatgct gagtcaaccg atggagaatt agaaacaatc tacagtaggg aagaaccatt
     2701 gacacaaaat tattgacgct cccaactaaa agtgtcaaaa taatgatgaa atcatttagt
     2761 tcttataaat tagaaaagag aaaataaata ttccatatct actttattgg atccgtaaca
     2821 tgactaaatt ctgaatactt tttaaagctt atcatttggt aaaactgaat ataagttaat
     2881 tatatagact gaaaattttg aattttcaga atagaataac ttaaaagtac tgacaaaatt
     2941 ttttaaaggg gcaatattaa tgttctgaga taatcaaatc ctaccgaaaa tatcaaaaat
     3001 ccaatgtgac acaatcagat tgtgtctcat tcgagggaat cacacaatcc cacgtggctc
     3061 taggcagaat tgaatttttg cttggggctt tttccttata ttgattttga atgagactta
     3121 ttatatgtta taatgcaatt aagtatcatt ggagagggcg aagaaaatga agacggtaga
     3181 acctattcga gacaaaaaaa agatcgatgc gatgaaggca atcttggctt cgggaaaata
     3241 tggccaacga aatttggttc ttttttcgat tgggatcaac accgcctatc gcatttctga
     3301 tttgcgtcaa ttgaagctgg ccgacgtgct ggaaatttca cgcggtcgcg tggtggtcaa
     3361 agatcgatta gcgatgaagg aacaaaaaac agctaaacat aattcggtct atatttccaa
     3421 caaattgcga aaaacgattt tggagtacgt tcaacaggaa tttcctgatc aactccttca
     3481 gcaagatttt gacggttatt tgttccctag tcgaaaaggg acgaatgaac cgctcaaccg
     3541 ccaaagtctg tggcgaatca ttcatgatgc tggcacggca atcggattga aagacattgg
     3601 cccacattcc atgcgaaaaa cgttcggtta ctttctctat aaacagggga ccaaaacaga
     3661 aatcattcag tccttgttga accattcttc tcagcgcgaa acattgcggt atattggtat
     3721 tacacaagaa gacaaagata cagctgtaaa aagcttagat ttataatata aaagttaagt
     3781 ttaaacatgg atagttttaa attagtagat tgagtgttgg gaggaaagaa tgagtacatt
     3841 atatattata ggaaacggtt ttgatttaag tcatggttta aaaactagat acactgattt
     3901 ttcaaaatgg gttgaaaaaa gtcatagtga aatattcgat aaaatagaca caatgataaa
     3961 tgaaaataat gaggtgagaa gtacagctga tgataagata ggttggttta attttgaaag
     4021 tagtttgcat gagttaattc aaagtccagt ctttgaagat tatgtagaag agtctaagga
     4081 ttatctcata ggttatggac atgaagattg gtcagattct gcccatcatg attttcaaaa
     4141 tatggtttat gattatgttg tttctctaga agaattacaa caacttttca aggaatggat
     4201 acgcaaagaa atacagccga caatagaaca taaaattcca aaataccaat tttcgtccga
     4261 tgtactattc tttacattca attatacaga tgttttggaa attctataca agcaaccgaa
     4321 agaaaacata tatcatattc atggcagtta tgacgatata ataatcggcc actccgagaa
     4381 ttttgacaac tttataggag a
//
LOCUS       DACCDF010000125         4374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCDF010000125 DACCDF010000000
VERSION     DACCDF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.133"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(83..1051)
                     /locus_tag="IVK51_002621"
     CDS             complement(83..1051)
                     /locus_tag="IVK51_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6432161.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQ
                     LRRGKATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYK
                     LRDILKRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
     gene            complement(1498..2904)
                     /locus_tag="IVK51_002622"
     CDS             complement(1498..2904)
                     /locus_tag="IVK51_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP6432162.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3126..3488)
                     /locus_tag="IVK51_002623"
     CDS             complement(3126..3488)
                     /locus_tag="IVK51_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432163.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3492..3800)
                     /locus_tag="IVK51_002624"
     CDS             complement(3492..3800)
                     /locus_tag="IVK51_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432164.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3809..4228)
                     /locus_tag="IVK51_002625"
     CDS             complement(3809..4228)
                     /locus_tag="IVK51_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432165.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4221..4361)
                     /locus_tag="IVK51_002626"
     CDS             complement(4221..4361)
                     /locus_tag="IVK51_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432166.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1234 a          994 c          619 g         1527 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tcttattcct cctccgttac atctaaccct tgaagccaag
      121 tgtctaattc ttttttcaaa acatctgctg attccgaaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcttttca aaatatctct caatttgtat ttcattctac
      241 gctctaaaaa cccccacatt tcagggttag caatcagtcc aaaaatcatc accttatcat
      301 ttgtttttac atcaagtggt agaaaatgag ggctagcaaa actatttaaa ctttcatgaa
      361 ccattgcatg ccaatctgtt gctttcccac gccttaactg caattccagt cgccaccaag
      421 tttctatttc tggtggcaca attttacgct tggtttcctg ttccaatttt ttgttgtata
      481 gccgaatctg gcgttcgctg gcacgactgc cccaataagc tgtttccagc ttcccagacc
      541 gcccataaat tggcttgaaa gaaacaggat caaccactcg atattgtgta ataaactcat
      601 caggcacatc gataatatcg caagcaatat ccgcacgtga gaaatgtggt tctagaaata
      661 aatcatgaat gaaatttttc attgaactag ctaaaaaatg accaatctta ttcgggttaa
      721 aatcaatccg ccctttgcca tcttgccatt tcaaaatctc aatataagca atattttctt
      781 cgtattggtc taataattgc cagccgttat tcccaaccgc ttttaaatag ccattgcctt
      841 cattcacccg cattaattgc tcaaaatcta aaatcaacac atcattttga gtgtgataat
      901 aaatattttc cttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggggtgta catattttgt acatttaacc gcctgttaga tagggctgtt aaattttccc
     1021 actcatttac gattatgcca tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactatttt tttactcttt
     1261 tttcagttca tttgtttgct aactcaataa tagcacttct ctttagctat gcaaatatcc
     1321 aaaaaataaa accgcaccct ttgaaaaggg cgcccccaaa cttcgctaac gctaggttgg
     1381 ccaaaaaatg gactggccat tttttagcaa atgtcagctt aataggctag tggtttagcc
     1441 gtcaactcct tatcctcggc acaaaccgcc tccggtaccg ttgactgcac atcgtaacta
     1501 ccactcgatc tcatcttcag aacgctctaa tttacgctgt gagacgtttt gacgataaaa
     1561 taaggttttc tcctgctcta acacttctaa ttcctcagaa agccccatat ccttttctgc
     1621 aagaccaaaa cggatagaca aacgagtgat cgatttttga aatgcgtttt ttaatcgctc
     1681 taaacgctgg cgtaaagagc gattttcttc tttcagcttt ttattttcca cttgtaaatc
     1741 tgctaattgt tttctaccag acaaatgacg ctcttcttcc aatgtagcta catactcatt
     1801 taattctgta ttttgctcaa gcatctgtaa atattgctca ctcatgttat cagcatttct
     1861 cttggcattt gttgctctcg tagctcgttg atacaaatca acaaaatcat tcccagataa
     1921 atgatacgaa ccatcaggaa gcctcttgaa actttctaaa ggaaccatat ctttttggaa
     1981 aatgaccgcc atctcttttt ttacattcag catatttttc ttctcttctt caatcttcgc
     2041 tctctcagaa gccatcagag ccttatcacg ctcgttttct tctctttgcc tagaaatata
     2101 cctcaactca tcagaaacgc tctcacgctc ttttacagcc gtttggcgca attcagaaat
     2161 ttcactcatg atttctttgt attcgtgaat atctttaatt ttgttggtac cgacaatatc
     2221 ccttttccaa ccgtaatcat tcattaatcg ttctatagag tcaacttctt gatttctaaa
     2281 attattgaaa agcactcgac cattatcttt atcagcgtgt atcccttgtt gaaccagtgc
     2341 tttattaaag ctcggctgct tttgaacacc ccgtttataa ccttcagcaa ccggcacaat
     2401 attcaagtgc aggtgcggac ttatttcgtc attatgaata acagcattat aaacagccaa
     2461 attagggttt ctctcttgaa actccttgta atactgaacc aaaattttat tggcaatcac
     2521 tcgattttgt tcagcttgtt gtttcgatat gccagtagaa gaaccatctg ctcgatttgt
     2581 ctggaaatca tctaaaatgc ctatttgcac aataaattct cgttgaggtt ccaacttttt
     2641 atccttgcgc actttcgcta aatagtcctc gatttttcta tcggctcttt tttgtttcgc
     2701 attatatttt tctaccactt cgccaaacaa ttcctcatat ttatctgcta gattcgtctg
     2761 ctctaaataa atattttcat ccgatttcgt gaagtcaata tgcttatgcc aatcattggt
     2821 tttctggcga tcagttaact cacgattatt atgagaaata cttgtattct tcgttccttt
     2881 tttaaatgaa atactaaacc ccaagttaaa accctccttc gcgctgtcgc taataaaaag
     2941 attgtaaacc tttctgttac tctttgtcaa agagtaacat tttcctaatt gtgacatggc
     3001 tgtcacaatt ccaaatgctc tccgagggta ttcgctgtca caagtgacaa cgaaaaaagg
     3061 agccgaaagc gtgtcacgct ctcaactcct tttacgcttt attttgcttc acgcaaaata
     3121 atcgcttatc gtaaattcac gtcattaaat attgacgtaa atttactctc actcatctga
     3181 cttttcttat tcggtatcac tccgaataag ccgtcagatt catcgggata atctcgttcg
     3241 cttggcgctg atttttcatc agcaagccac tcattatcca ctgatttttc gtgttgctga
     3301 tcgtcagaaa tcgacgatag ccaccgctca aaatcagcaa aagtttcaaa cccaaaacgc
     3361 ttgcgaagag ccttgcccat tgcagcattt tctttctcaa tcaagcgttt tttctgttgg
     3421 cgaagtttcg cttgttcttt ttttaatttt tcttcttgcg ctttaatatc tgctaaagtc
     3481 attcccataa actacatctc cttttctttg gaattaataa cggaaatttg gtttaactgc
     3541 ataacatgac tcattaattt caaccaaaat tctttttctg attcatcagt tgtttgcgct
     3601 aaatgcttat gaacaaattc attagtcaaa actgaagatt ttgctagatc agcattgata
     3661 accgagttga gaacatccat cattcgttca agcatttcac tataattatc ttcatccggc
     3721 aaattcaaat aatcagctaa ccgttcatac atcaattcaa ttctatcgac tgtttcttct
     3781 ctcaaatcaa ttttatccat gtcacacctc acttccagaa ttgccaccat tttcgatttt
     3841 ttaattcttg attttctagt aacaactgat cgtttttttg ttgctgaagc aaagtcaatt
     3901 tttgttggtc acgaatcatt tccgttaatt cagaaatatg cctatctttt tccgctaatt
     3961 gttcacgcaa aaattcattt tctttttcta acgggaaatc attgcctgca tcattgaccg
     4021 gtaaatcacc agtcaattga ccaccttttg accgataaat tgaccgcaca acaccaatca
     4081 attccttgtc taacatgata accccattat ctttataccg agcaaggtca taacctgcat
     4141 tgaccagttg attgcgcact gtatttttag atatacccaa ctcatcagca aggtttttta
     4201 tggtcatttt ttctggcaaa tcagtcatta tcttctccgt tactttcagc taccccataa
     4261 acatatttaa cagcatgctt agggataatg attggggaat caattttatt atttttcaaa
     4321 atcaactcat actgcgttac atcaagtaac ttaccggaca tttttttgct atcc
//
LOCUS       DACCDF010000126         4362 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCDF010000126 DACCDF010000000
VERSION     DACCDF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.134"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            149..1072
                     /locus_tag="IVK51_002627"
     CDS             149..1072
                     /locus_tag="IVK51_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6432167.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1090..2331
                     /locus_tag="IVK51_002628"
     CDS             1090..2331
                     /locus_tag="IVK51_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP6432168.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            2465..3811
                     /gene="gor"
                     /locus_tag="IVK51_002629"
     CDS             2465..3811
                     /gene="gor"
                     /locus_tag="IVK51_002629"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP6432169.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
BASE COUNT         1502 a          689 c          940 g         1231 t
ORIGIN      
        1 caatctcttt ttatatttgg acagaatata tcgaacttta ttttgatttt ttgtgaaata
       61 attttcaaaa atccgacttt aaatagaaga agtattggaa agtttttaga gctatgctac
      121 tattatgaag ttgtaggagg agaaaattat gatcgaactc ataaccacag tagaaactat
      181 tgaacaagca gaacagttgc ttgctgcagg tgcagataca ctatatttcg gtgaagagca
      241 atttggattg cgtctgcctg cttcatttac acgagaagaa cagcgacaat tggtagaatt
      301 agcacacaag tatggtaaaa aagcgaatat tgctgtaaat gggatcattc atcctgaaaa
      361 aatgaaaaat gtacctgaat atttggcttt tttaaaagaa ataaacgtag atgtgatcac
      421 agttggtgat acaggtatta tttatacgat gaaaaaaaat cctgaattgc gtataccgta
      481 tatttatgat gctgaaacat tagtaacgag cgcacgtcaa atcaatttct gggcgaaaaa
      541 aggcgcagtt ggagcagtac ttgctcgtga agttcctttt gaagagatga aggcgatgga
      601 agaaaaactc gatattcctg tagaaaccct tgtgtatggt gctacatgca tccatcaatc
      661 caaacgtcca ctgctacaga attactataa ctatacgaaa caagacgaac aaaaagatcg
      721 tgaaagaggt ctttttatct cggaacctaa aaaagaagag acacattatt ctatttatga
      781 agatagtcat ggcacacata tttttgccag caatgatctg aatctctcga atgaattgaa
      841 tgaactttat cagcatcatt actgtacatg gaaattggac ggattattta ctccaggcga
      901 aaaattcgta aacatcactc gactattcgc cgaagcaaaa aaacaaattt tagaaggtag
      961 ctgggatcaa gaaaaagcgg aaaaaatgac tagcgaaatc agtaaattgc atcctgaaaa
     1021 ccgtgaatta gatacaggat tcttttatat tgatccttct gctatcaaat aaggagtatg
     1081 atgaaagaaa tgacagtaaa aagaaaattg aaacgaccag aaatcctagc gccagcagga
     1141 acactggaaa agttaaaaac cgcgatccat tatggagcag atgcagtata tattggcggg
     1201 aatgcatatg gtttaagaag ccgtgcaggt aattttactt atgaagaaat ggctgaagga
     1261 gttgcttttg caaaagaaca tgatgcgaag gtatacgttg ctgctaatat ggtcacccat
     1321 gaaggaaatg aagatggagc aggggaattt ttccgcagtt tgcgtgatgt cggtatttct
     1381 gcagtgattg tttctgatcc agctttgatc gaaatttgtg cgacagaagc cccaggtctg
     1441 ccgatccatt tatccactca agcttcagca actaattttg agacattgga attctggaaa
     1501 gaagaagggt tggaacgtgt cgttttagct cgtgaagttt caatggagga agtggcagag
     1561 attcgtaaaa atacggatac cgaaatcgaa gcatttatcc atggtgccat gtgtatctct
     1621 tattctggtc gatgcacatt gtccaaccat atgtcgatgc gggatgcaaa ccgtggcgga
     1681 tgttctcaat cttgtcgctg gaagtatgga ttgttcgata tgccttttgg tcaagaacga
     1741 aacagtttaa ctgatacagg cgaagtggaa gaagaattct cgatgagtgc agtggatatg
     1801 tcaatgatcg aacatattcc agaattgatt gaaaacggcg tagacagctt caagatcgaa
     1861 ggaagaatga aatcgatcca ttatgtatct acggtgtcaa atgtctataa agcggctgta
     1921 gacagttata tggaagatcc tgaaaactat gtctgcaagc aagaatggat cgacgagtta
     1981 tggaaagtag cacaacgaga attagctaca ggtttttact acaatacacc aactgaaaac
     2041 gaacaattat tcggtgaacg tcgtaaaatc ccagtatata aatttgtcgg tgaagtatta
     2101 tcttatgatg aggcaacaca gattgctacg atacgtcaga gaaacttgtt ttctgtagga
     2161 gatgaaatcg aattttatgg tccaggattc agtcatttca gtcaaaccgt tgaagttatg
     2221 cataatgaag aaggcgaatc cattgaccga gcaccaaatc caatgatgat tttaactatg
     2281 ccagtcgcac agccggtgaa acctggagat atgatccgaa aacgtaaata aggaaaaagc
     2341 tgatatatcc ctgttttaag catcttcgca tacaaaattc aagaaaagtg ttacacttgt
     2401 tttctgtaga aaaaggatcg ttaaataaag gttttgattg atctcttcaa taaggaggaa
     2461 caaaatgcaa atatatgatt acatcgtaat tggtggcggc tctggcggta ttgcttcagc
     2521 aaacagagca gggatgcatg gcgcaaaagt tttgctgatt gaagcgaatg aattgggcgg
     2581 aacatgtgtc aacgtcggtt gtgtacctaa aaaagttatg tggcaagcaa gtacgatttt
     2641 ggaaacaatt gaacgagatg cctcaagtta cggtatccaa gcagatttaa aaaatgttga
     2701 tttcgccggt ctagtagaaa gacgagaaaa atatattgat tttctacacg gtgcttatca
     2761 aagaggatta gatagcaatc atgtcgaggc tataaaaggt tacgctactt ttatcgatag
     2821 tcaaacgatc gaagtaaacg gtgaaaccta tcgtgctcct aaaatcttga tcgctactgg
     2881 aggccgtgca agtaaaatga ccattcctgg tggagaatac gcaattgatt caaatggatt
     2941 ttttgaatta acagagttac ctaagagagc aatcgtttta ggtgctggat acattgcggc
     3001 tgaactttca ggtgtatttc gtggtttagg tagtgaagtc atgtgggcat atcgaaaaga
     3061 acgaccactt agaacgtttg ataaaatgct ttcggacaat ttgatccaaa tgtaccaaga
     3121 agctggaata aaaacttacg cacatcatat tgctaaagaa attactaaaa acaatgacga
     3181 atatacggtg ctatttgaaa atggtgaaac cttaactggt gattgtgtac tttttgccgg
     3241 tggacgtgta ccgaataccg aaaaattagg tctggaaaat acaaatgtgg aattggacaa
     3301 aaaagggttt atcaaagtag ataaatacca aaatacaacg gatgaacata tttttgctgt
     3361 tggtgatgtg atcggtaaat tagatctgac tcctgttgca atcgcagctg gtagacgttt
     3421 atcagaacgt ttatttaatg gaaagaaaga ttcttatctt gactataaac ttgttccgac
     3481 agtcgtcttt acgcatccac caattgcaac aattggtttg acagaagaag aagcattgga
     3541 aaaatatggc gaaaatgaat tgaaagttta tcgttcacgc tttaccccaa tgtactttgc
     3601 attaaatgat tatcgccaaa aatgcgaaat gaaattgatc tgtgtcggaa aagaagaaag
     3661 aatcgtcggc ttacatgcta ttggtgttgg tgtcgatgag atgctgcaag gttttgccgt
     3721 ggctatcaag atgggtgcaa ctaaagaaga ctttgacaat acagtagcca tccatccaac
     3781 aggagcggaa gaattcgtaa cgatgcgata atttaatatt aacagttcca acagcttttt
     3841 attgaacaaa ccatgtaaaa ccagaaaatc agtatactga ttttctggtt ttttgttttt
     3901 tagttattta tgtaagattg cttcgataat tctcaaaacg aaataagtag ggaagttgga
     3961 gattttgatt atcttcgtaa ctgacgaaga gtaaatgaat ccatttggta tgcactctta
     4021 agttcaaaat attggtatcg atgtttaaaa gaaatagaaa tgtatcgaag aacagatgtt
     4081 taaaaaagtg tgactttgta taaataaatt agaagaagca aacgaggatg ttaaaagaat
     4141 accatatagt taccaaacca agaaagttct cgtcaagttc gctggtacga atcaaatcaa
     4201 gtgaaaaaag ggactgtgaa aaaaattgtg tcatagttag tcactttttc cgaacaaata
     4261 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     4321 tttccatttg tttctctaat actcaggaca aaacactgat tt
//
LOCUS       DACCDF010000127         4223 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCDF010000127 DACCDF010000000
VERSION     DACCDF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.135"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(384..1670)
                     /locus_tag="IVK51_002630"
     CDS             complement(384..1670)
                     /locus_tag="IVK51_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6432170.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1663..2931)
                     /locus_tag="IVK51_002631"
     CDS             complement(1663..2931)
                     /locus_tag="IVK51_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP6432171.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3183..3644
                     /gene="mscL"
                     /locus_tag="IVK51_002632"
     CDS             3183..3644
                     /gene="mscL"
                     /locus_tag="IVK51_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP6432172.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3703..4149)
                     /locus_tag="IVK51_002633"
     CDS             complement(3703..4149)
                     /locus_tag="IVK51_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP6432173.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1196 a          862 c          740 g         1425 t
ORIGIN      
        1 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
       61 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
      121 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
      181 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
      241 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
      301 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
      361 acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct
      421 tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt
      481 tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata
      541 tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg
      601 agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt
      661 tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga
      721 tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga
      781 tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag
      841 cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat
      901 ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca
      961 gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga
     1021 ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt
     1081 ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt
     1141 tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg
     1201 ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt
     1261 cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga
     1321 gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa
     1381 cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct
     1441 tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt
     1501 tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa
     1561 ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt
     1621 acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc
     1681 tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt
     1741 gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag
     1801 ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata
     1861 ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg
     1921 caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat
     1981 accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc
     2041 tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa
     2101 accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata
     2161 agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt
     2221 cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa
     2281 ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat
     2341 ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc
     2401 caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag
     2461 ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag
     2521 acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt
     2581 tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt
     2641 gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt
     2701 tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt
     2761 ctgatccgga taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt
     2821 gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg
     2881 ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg
     2941 ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact
     3001 tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta
     3061 cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata
     3121 ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat
     3181 tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg
     3241 gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca
     3301 caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac
     3361 tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta
     3421 gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa
     3481 accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg
     3541 cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata
     3601 cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa
     3661 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
     3721 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
     3781 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
     3841 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
     3901 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
     3961 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttg
     4021 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
     4081 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
     4141 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
     4201 aaaagcaaac gaaccttacg aat
//
LOCUS       DACCDF010000128         4222 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCDF010000128 DACCDF010000000
VERSION     DACCDF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.136"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..343
                     /locus_tag="IVK51_002634"
     CDS             <1..343
                     /locus_tag="IVK51_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432174.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            424..1044
                     /locus_tag="IVK51_002635"
     CDS             424..1044
                     /locus_tag="IVK51_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432175.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     EIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQIS"
     gene            complement(1374..2600)
                     /locus_tag="IVK51_002636"
     CDS             complement(1374..2600)
                     /locus_tag="IVK51_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6432176.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2672..2830)
                     /locus_tag="IVK51_002637"
     CDS             complement(2672..2830)
                     /locus_tag="IVK51_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6432177.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3518..>4222
                     /locus_tag="IVK51_002638"
     CDS             3518..>4222
                     /locus_tag="IVK51_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP6432178.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1449 a          590 c          689 g         1494 t
ORIGIN      
        1 aaatataaaa actagtacga aattaatttg taaattattt aagcttgata aaataagcaa
       61 tatcgatgat gtaaagtcca agatattaac aagaaaagta agcgaaccag taaagctaac
      121 ggcacaagat aaaaaaaatg gaattgttct ttatggtgat gatgcaaagt ataactataa
      181 ttgggatacc tcctcagaag ctgaggtatt aagtattcct actaccgata cttcagggga
      241 aaaagtcacc tttaaaataa attttataac ggcaatttct aagctggagt atgggaagca
      301 aaaaaagaga gataacaatg atcaatatct acctaaaaaa taacgaatta atgatgcata
      361 tgttaaagtt gtttttttga attaaacaac tcaatttatt cagttatatg ttcatcgcag
      421 caagtgaaca ttaatcgagt tataaaatta ataggagcta aaaatataaa taaagttcga
      481 tcagaattta tttttccaga agattttttt aactggtttt tttatcaatt cagtgaatgc
      541 aagcgactct taggtgagaa ttattactta aaaggaatcg gcggatttat ggggaatgtg
      601 gctgatgaac aaaatattat aaaaggaata tctgaacaaa catcagagct tacagtaact
      661 aaagcattta taagtaatgg tgaaagtttg aaaaatgtaa ctgcgcgaat tagaaaagaa
      721 aatgaagtag aaattgtttt tggaattgac catgaatgta attcagtgat atatgtaaca
      781 caatcagcaa atttaattct ttttgagaat ggttgcaagg acgattttat ggtggtctat
      841 ttgtacgata tactaattcc aaaacttcgt tccttatata tgtcttctga aagtattttt
      901 gtgagtgaac aaaagggatc tttctcgaaa aaaataggta ttgaagtaat tgaatcgatt
      961 atgtgtcata atcatattac gctagaagaa ttagaaattt tgatagggca aaaaaataat
     1021 aacacgaaaa tacaaatttc ttagaaatca ttttttattg agatatctag ataaatgatt
     1081 tattcttcaa tcaaccaact cgccaagctg aggtaatgtt ttgaggtaat gataacatac
     1141 aactttttat accctagtaa aactctataa acaaagactt attaatttcg catgaaatac
     1201 attcctgtaa aatgcaataa aatagagttt tgtttaacca ggtctcaaaa aggctcgtaa
     1261 agcatctcaa ttctctaaac gttaatatta tttatatatc aatgtttcaa ggcacttttc
     1321 aattgtttga aaagtgtctt tttcttgttt aagaacacat ttaagaacac atactaatca
     1381 catttattca atcctctctg aaaggcttca aaaacagttt tatctactac atctggtgta
     1441 tttacgtata ttctcgttgt agaaatatct gagtgtgtta aagcactaga tatttgattc
     1501 atatttgctc cgccttgtct agctatagtt gcataggtat gacgaagctt atgtggagat
     1561 aaatgagcca attcaggatg tcgtctttta attgaattga ttcgatagtt taggtaatca
     1621 gcgtgaacag ggcaatttac ttctccggat ggtttagtat aggtaaagag atactgatct
     1681 ggtgtttgat taatttttaa tctcaacaat tgctcagctt gcactgattt ccatttgctt
     1741 agtaatgcca ttacaatttc gggtacttga attacggtat cctttcgacc ttttgtaaat
     1801 ttcttttttt gatatttatc caaagcatgt ttaaggcgaa ccgtttgttt ctcaaagtcg
     1861 atatatttcc attgcaaagc atacgtctca ctttttctat caccaagata tagggtaagc
     1921 atgaaaagag tatagtcatg aagagtaagc aagtgattat tcaagtcagt gtttactgct
     1981 tctatccaag caataagctc ttctgacgat aatgcttgat taccttcccg tatccttctt
     2041 tcttctaaag caattttttt aggtgcagtg atacattgaa taattttggt agttcggtta
     2101 aattcaatat aatttagtat ttctgcaata tcaaacatag aacggacata tcctttgata
     2161 atttttatat ttgcgtattc tttcgatttt tttgttaatt catctgatat tatctcagta
     2221 tttgaattta gatagttcat tgcatactca ccaaacattg gaaggatgtg caatcggaat
     2281 atatctttag tattactaat tgttacgtaa cttggagctc tttttgatct agtagtctga
     2341 cctagttcat atctaggaag ccattttgat tcatagaatt ccttaaattt tatttttcct
     2401 tttaattcta gtgaatttgc attctcctct cttaaaactg tttcaatttt ccttttcatt
     2461 gcattttcgg ctttaatagc caattgccta gttggaaata ctttttgata cttttctgat
     2521 gtaattccag tgatttctct gaattttttt ggatacctca gattagtttt atatttccca
     2581 tttgaaagtt ttgtaggcaa aattcctcac tcccttaaaa agtaaggcag tatatttcgc
     2641 tattcttagt atatgcggcg agaagaaatt ttcaaattgt tttaaatgta ttcataaaat
     2701 ctttgatatc ctctatgtca aagcgaacta taccgtcaat tctgattact ggaaaattat
     2761 atatacgaat ccacttatct aaagtattat tatcaattcc tagaaactca caggtttgtt
     2821 ttttattcaa gtagactctc aattttataa aatcatctcc taaaaaagat tagaattttt
     2881 gaattgcttt gggtaagcac ataggaattt cacctctccc cagtttacgg cgagccttac
     2941 ggaagtatca ttatttgctg ctattgtcat ggcattttgt tttcccagaa caaaaagaaa
     3001 tctctaagta tttgtcgcta atcaggtcat ggcgtacttg agtttctagc tcgaatagca
     3061 gggatcgtta ttcaaaaatt gtatttaatt gtcaatgatc aaaaataggt tttttcctat
     3121 tcactattaa taacgataaa catgtataaa aagacacact acgaaaaaag ttaaatggag
     3181 tttactgatt ttcatatttg tatatggaac agacatagtc ttgaataatt tttataaatg
     3241 ttttgatgct tatcaactta ttcaacccgc tatccacttt aagtataatt tttccatacg
     3301 ccaataaagt agttttagga gcatgttctc atttgagaac gctccttttt tatatatttt
     3361 gaaagctact tactatacgc gtagtttcaa atagttttgt cattctaatc ttcagcaaca
     3421 gaaaaaaatc taaattataa aatttagggt tgactaaaat ataatttttg tataccatat
     3481 gatcgtgata atcattatca tttagaagga gaaagttatg ttagaagtga aaaagcttaa
     3541 tgtttgttat aacgattttt tagcattgaa cgagatttct ttaaaaatac aagaaggtgc
     3601 tttaacaggt attattggtc caaatggtgc tggaaaatct acattgctta aagctatgtt
     3661 aaatattatc cctcatcagg gagaggtctc cattaataat gaagatatca ataaaaaact
     3721 ggtgaagatt gcatatgtag agcaaaaagc tgatattgat tttacttttc caataaaagt
     3781 aaaagaatgt gtatctatgg gaacatatgc cggaatgaaa gtttttcaaa gaattaaaaa
     3841 tgcagaatgg caaagagttt caaaggcttt ggaaaaggtc gatatgggaa aatattctaa
     3901 tcatcaaata ggagaattat ctggaggaca atttcaacgt gtacttttag caagatgtct
     3961 tgctcaaaat gcggatttca tatttttaga tgaaccattt gtagggatag acttagttag
     4021 tgaacgaata attatggata cgttaaaaga attgaaacat cagggaaaaa cagtgctaat
     4081 tgtgcatcat gatttaagca aggtaaagaa gtattttgat aatattattc ttttgaatcg
     4141 aaatctaatt gctcacggaa gtgtagaatc tgtatttaat gaagagaatt taaaaaaagc
     4201 ctatggagat actatattta tt
//
LOCUS       DACCDF010000129         4218 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCDF010000129 DACCDF010000000
VERSION     DACCDF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.137"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            10..210
                     /locus_tag="IVK51_002639"
     CDS             10..210
                     /locus_tag="IVK51_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP6432179.1"
                     /translation="MALGRMSELGYSMMFGEVEKNFFMKHIKGRGYVDTGGSVISEFY
                     TPLVPKGYDFLDSISKLEKMND"
     gene            288..962
                     /locus_tag="IVK51_002640"
     CDS             288..962
                     /locus_tag="IVK51_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6432180.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            1195..1629
                     /gene="dcm"
                     /locus_tag="IVK51_002641"
     CDS             1195..1629
                     /gene="dcm"
                     /locus_tag="IVK51_002641"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6432181.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            1630..2337
                     /locus_tag="IVK51_002642"
     CDS             1630..2337
                     /locus_tag="IVK51_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP6432182.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            2327..2617
                     /locus_tag="IVK51_002643"
     CDS             2327..2617
                     /locus_tag="IVK51_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432183.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            2876..4060
                     /locus_tag="IVK51_002644"
     CDS             2876..4060
                     /locus_tag="IVK51_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6432184.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            4075..4194
                     /locus_tag="IVK51_002645"
     CDS             4075..4194
                     /locus_tag="IVK51_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP6432185.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         1364 a          692 c          979 g         1183 t
ORIGIN      
        1 aactttcgcg tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag
       61 gtcgaaaaaa acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc
      121 agtgtgatta gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca
      181 attagtaaat tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa
      241 gtaacgtata atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg
      301 attcttattt agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag
      361 ggaaatcccc cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa
      421 aatcagaggt tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg
      481 attactttcg cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag
      541 catcttcttg gttggtagag caggcattta gatatgtctt agatcatggc tattctttta
      601 ttctggatgc aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa
      661 aacattttcg agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta
      721 cacaagaacg tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt
      781 ttttcaaatc aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc
      841 atatcataat aaaagattat acgaataata tttccgaagt tcattatgct acggataatc
      901 tagaattatt tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact
      961 gagaaagaac aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg
     1021 gaaaatgcta ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat
     1081 gaaaaacaac ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat
     1141 gagccaagag caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga
     1201 tttttagatt tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat
     1261 cattgtatcg gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat
     1321 gatacaagta aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa
     1381 tccctcggac cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg
     1441 ggaaagcggc aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc
     1501 accactcttc tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac
     1561 gaaggagggg ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg
     1621 gagtggtagg tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt
     1681 atcggacatt ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca
     1741 acatctgatc aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt
     1801 ggacaagtct atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa
     1861 atcgcactgc cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa
     1921 caaggtaatc gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt
     1981 ggtacaaaaa tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac
     2041 agtgtgaatc tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt
     2101 gttcatacct tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg
     2161 aagctaacac ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga
     2221 gattctcaag tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt
     2281 ccggtcattt ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga
     2341 gttgattgga gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga
     2401 acaattgcga cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc
     2461 atatttaatg aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt
     2521 tgagcggatt gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa
     2581 agcagagttg gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt
     2641 taggcgctga ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca
     2701 tggacggcgt tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac
     2761 acaaaaaata agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt
     2821 gtataggtgt taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc
     2881 acaacggaat ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca
     2941 aaataaattg gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg
     3001 tggcgtaacc gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa
     3061 tcccgatttg cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga
     3121 tgcacgaaaa attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga
     3181 ttatgccttt tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc
     3241 tcatgaagaa gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga
     3301 aactttttta ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc
     3361 cggtggcgtg atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat
     3421 acctgagcta acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa
     3481 aagttatcgt tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt
     3541 atatatcggc agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca
     3601 atatatcaag ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg
     3661 actgaaaaat gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga
     3721 gagcaccacg ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa
     3781 aaggcgaacc aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa
     3841 agagattcaa ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct
     3901 aggccgacaa gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac
     3961 aacctatatc caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt
     4021 ggagcaacaa agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga
     4081 attgtaaaag atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc
     4141 ttgatgaatg caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat
     4201 gaatttcgga caaaattt
//
LOCUS       DACCDF010000130         4215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCDF010000130 DACCDF010000000
VERSION     DACCDF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.138"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            624..3263
                     /locus_tag="IVK51_002646"
     CDS             624..3263
                     /locus_tag="IVK51_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP6432186.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3431..4129)
                     /locus_tag="IVK51_002647"
     CDS             complement(3431..4129)
                     /locus_tag="IVK51_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP6432187.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIAAILLIMIPI
                     ILFATTIYDGSASVSDATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVIGLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
BASE COUNT         1278 a          805 c          911 g         1221 t
ORIGIN      
        1 ttctttctgt tctgtccaaa ttattctaag atttctcttt accaacatca tttgacaaag
       61 agccagaaaa agccgagctc tcgataagct cggctttctt gtacctcgga aggattcaaa
      121 taaacagaaa tgattgtaac tttccatcat acatgcaaag cacggtgttg aatttcttca
      181 tgttaaacaa aaattttgtt ttcgttacat cttcaacatt tgaaacatct cttgttccta
      241 ttcaattttc agacgtttac gttattcaat tgttacgtat cgcattactt cttgtttcgg
      301 atacttcgtg atccaaacat ccatacgcat tcactattta taggtttgta ctctacacgc
      361 atgtctgggt gaaacctgta cttctaacaa ctttatttgc ttacactgtt ctatcgagaa
      421 attcttctgt ctatttgatg gtatcctcct tccttacatc tatataatag catcctttgg
      481 tagcgtttgc aaatataagc actttaaatt ttacgctttc ttaacaaata atcgataagc
      541 cctttatttg aagtaaataa aaaaaggatg ttatagtaaa ataagtacct ttttttgtac
      601 gaagatttta aaggaggaat aggatggaag aatacaagca gacaatagaa gaagtaaaga
      661 aggtaacaga agcagatcca gatactgggt tgtcttcaca agaagtggat gaacggcgaa
      721 aaaagcaggg attgaataaa ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct
      781 ttcgaagctt atccgatttt acaacgatca tccttcttgt agcagcggtg atttcctttt
      841 ataccgcatt tgctacagaa catggggatt tatttgaagg actgctgatc atcgctatcg
      901 ttgtgatcaa ctcggtattg gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat
      961 ccttgcaaga tatgaataaa caaacggcaa ctgtcatacg agacggcaaa gtgaccactg
     1021 ttgagtcaga gcaactggta gtgggtgata ttcttgtttt agagtccggt gatgctattt
     1081 ctgccgatgc ccgtttgatc gaagcatcac aattacgtgt agaagaatcg gcgttgactg
     1141 gagaaagtga agcagttgaa aaagatgcag catttattgc tgaagaagat gagtcattag
     1201 gtgaccaact taatatggta tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga
     1261 ttgtcactgc cattggtatg gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa
     1321 atactatgca aaaaacacct ttacaggtcc gattgaatca gctaggcaaa cgaatcagta
     1381 tgatcgcttt agcggctgcg gctttagtat ttgtgattgg tgaactgcaa ggagaaccac
     1441 tccttgagat gttcatgact tcgatttcct tggctgttgc agctgtaccg gaaaccttga
     1501 ctgttattgt tacgttgaca cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga
     1561 tcatccgaca gttgccagca gttgaaacgc taggaaccgc taatgttatc tgctcagaca
     1621 aaacaggaac gctgacacaa aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg
     1681 aagtaacgaa tatcgaagat tcaatgacga acagcgcgat ggaaatattg aaaatggctg
     1741 cactatgtac agatgtgacg gtcgagcaag aagatgacga ccttgttatc aaaggtaatc
     1801 caacagaagt agccatcgta agggctgttg aagagaatta tcatacaaaa gcagaattag
     1861 aagaaaaata tccacgagta aacgagcttc cttttgattc tgaacgaaaa atgatgacaa
     1921 cagttcacca aatgggcgaa aaatacatct caatcaccaa aggagcattt gatgttcttg
     1981 ctccgcgctt tagttcaggc gatgtagaac aagcggcgat cgttaatgat cgctttggta
     2041 aacgggcact gagagtgatt gctgttggtt atgctactta tgacgaagaa ccgcaagata
     2101 tttcttctga agcactggaa aaagacttac gtttgattgg tctgatcgga atgatcgatc
     2161 caccacgtcc agaaagtaaa ggagcaatca agcgagcgaa aaaggctggc atcaaaacag
     2221 tcatgatcac tggagatcat gtggtaactg ccagtgccat agcgaaagaa ttaggtatct
     2281 tgaatgatcc gtcagaagca ctctcaggat cagaactgca tcaattatca gacgaagaat
     2341 tagatgcgcg tgtcaaagct ctctctgtct atgctcgggt aacgccagaa gacaagatcc
     2401 ggatcgtcaa atcttggcaa cgtacgggtg cagtcgtagc gatgactggg gatggtgtga
     2461 atgatgcacc agcgttgaaa gcaagtaacg ttgggtgtgc catggggatc acagggacag
     2521 atgtggcgca aagtgccgca gatatgattt tgacggatga caactttgcg acgatcgtag
     2581 atgctgttag tcaagggcgt tcagtttatc agaatattcg taaagcaatc aactttttac
     2641 taagctgtaa tatttcggag atctttattg ttttgattgc gatgctgtta ggatggggag
     2701 caccatttac agctgtgcaa ctactgtttg tcaatgtagt tgctgatgga cttccaggat
     2761 ttgctttagg aagagagcca gcggaacacg gcatcatgga tcagccaccc attccgaaaa
     2821 atgaagggat ctttgctcga ggtttattgc aaaaaattgc cataaatgca ggggtcttca
     2881 ccatcgtaac cttgtttggt tattatttag gtagttatgt cgatactatc agcccatggg
     2941 tagacgcaag tcagcatgtc ggtcaaactg ttgcattctt gatcttagct tattcatcga
     3001 ttctgcatgt gttcaatgta cgcagcagcc aatcgatctt caaagtcaat ttagcaacca
     3061 ataaagcttt ggtagaaatg gcgttactgg cattagctat tacaacggct gttgctttgc
     3121 tgccatttac gcaagaacta ttcggcctag tacacatcag tttgaatcac tggttcttgg
     3181 tagggatttt atctatcgta ccaatcgcgg tgaatgaact gatcaagttc catcgattac
     3241 cggaagcaga agaagaggaa tgattaactg tttctaagtt gattttcttt ttactggaac
     3301 tatacaaaaa ggaggtgtga catgaatgtt gtcacacctc ctttttgcta atgggctgaa
     3361 tgtttctttt actgcctctc gataaacggt gttctcctac caacccctcg gtcacaagtc
     3421 acactctctt ttaaatatct tttggcaatt gttcataaaa tgctgctttt gttgcggaaa
     3481 tgtatgggct gagccataga taaccaatac cagcagttgc taatgctaag atatgccagc
     3541 cgataaatga aagatctaac cagaaaagtt gtcctttata accatccatc aattttcgac
     3601 ttgcagtgat cgtatccagt actttaggtg tttgtcctgt ttcttggacg atatcgtagt
     3661 agataaaata gctttgagaa taagaatagc ttttaatgat tccaggaata actaatagca
     3721 aaccccataa tgttgtaaag atcgagatca gcacagctaa gccgatcact ccgccgataa
     3781 acaaaccttt gaatccacga aatgcatctt tgaatggttc gatctgatct ttttgtccac
     3841 gtaaaatatc aagataagtc caagaaatac cgctcatgaa caaagcgcta atgatgctgg
     3901 aaataattcc gccaccgcct cctgaactac cgcctcctga agttgtcgca tctgagacgc
     3961 tcgctgatcc atcatatatg gtggtggcaa ataagataat aggaatcata ataagaagaa
     4021 tagcagcaat accaatcaat gtagggatta gatttagtaa aacagcttgt ccccaacgtc
     4081 cttttagact ctcttttgct tggcgtttta attctgcaga agattgcatg atttttccct
     4141 ccttttcttt aataacacca atataccatg tatcaaaaaa tatttctgtc ataatgtttt
     4201 tctctttttt gttat
//
LOCUS       DACCDF010000131         4199 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCDF010000131 DACCDF010000000
VERSION     DACCDF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.139"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(2..1222)
                     /locus_tag="IVK51_002648"
     CDS             complement(2..1222)
                     /locus_tag="IVK51_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6432188.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1242..1847)
                     /locus_tag="IVK51_002649"
     CDS             complement(1242..1847)
                     /locus_tag="IVK51_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP6432189.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1896..2828)
                     /locus_tag="IVK51_002650"
     CDS             complement(1896..2828)
                     /locus_tag="IVK51_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP6432190.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLSNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3253..4110)
                     /locus_tag="IVK51_002651"
     CDS             complement(3253..4110)
                     /locus_tag="IVK51_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432191.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKNDTID"
BASE COUNT         1191 a          849 c          692 g         1467 t
ORIGIN      
        1 tttattttac agaagcactc gaaactgcat ctactgcagt ttcatcgtgt gtgctggcgc
       61 tggttactgc atctgttttt ccaccatgaa cagatgcact tgaaaccgca tcttcatttc
      121 caacaagttg ttgacccgtt tgttgtaaat accactcaat gattggtttg aaatcaagaa
      181 tgtattcatt gattccagca tactctccat tttcatcatg cattgcttgg taattatgca
      241 caatatattt ttctggcact tgttttggta catgaacacg taccgcatct gttgctccgc
      301 ttcgcagttg ttgaattacc cactctacat ttttatacga tttttctggg tgacagttgg
      361 ctaatggatt ccctacctga ccgggcacac gagcagctaa catctctttc gcttcttggc
      421 gcaaattata atataaaaat tggttattat tatctgcaaa agttaattcc atcggcatcg
      481 aatctaaaaa gtaattgatt tgatccactg tcaataaccc acgatttaat ttgacgtaag
      541 tatttccaga aacggcaccg actgcttcag aagctttttc caaccaatga tcatctgaca
      601 tatcaacatc gggtattgtc gtagcaattg ttcctgtcgc atgcaaatct tctggtggaa
      661 gctttttagg ccctgctaag gtagacacga ctttaatgaa ttttacaaaa cttgctaatg
      721 tactgcctaa aaagtcaaca gttcgtggat caatcaagcg atcttcttgg tcaaatgctt
      781 cttttgcttt tcccaataaa aattcatttc cgggcatcac taatgcattc acgcctggtg
      841 cgtccaaaat ttggcgcaag tgtaattgcg cgcgtgaagt tccttgatcg tagtaagaag
      901 cacccacaac cattacaggt ttattttcaa gtggatggat tttaaatgat agccattcaa
      961 gtgcactttt caatcctgct ggaaccgtat gattgtgttc tggcgtagca ataattaccc
     1021 catcagccat taagattttg cgatttagct gttgaatagc aggactattt gtttgatcat
     1081 catcttgatt gaacagaggt aaatctttga tttcaatgat ttctaaatta aatagttcat
     1141 gatattgttt ttggataaat gttaataatt tacggttata tgaaagttca gctgctgatc
     1201 cgacgatacc tattaatttc atctttgttt gctcccctcg attaaatcgt ttcccatgaa
     1261 aagctttcgg cttcacgttt atttgcttgg tgcgcatgtt gcaattgact agtgattgtg
     1321 acaaagagta agaaatcttt gaatagttca cgtaattttg cgacttgttc ttcatcaatg
     1381 agtcgtcctt gctcatcaaa ggcttggagt gaatgaccca ataaaaattc tgagcttggc
     1441 ataattcgtg cctttaactc tggtgaatcc aatatttggc gcaaatgtgc ttgtgcgcgt
     1501 gaagaaccta acgtgccata agacgctccg gtaatcatca ctggtttatc cacaaacgga
     1561 aaaataccat atgaaagcca atttaatgcg ttaatcaatg aagcaggaac cgcatggtca
     1621 tactctggtg tgctgatgat tacgccatct gcttgttcaa tttttccagc aatttcttta
     1681 acaacatttg gtagttctgt gttttcaggt ttgttaaata gtggcagctc cttgatttcg
     1741 acaatttcga tttctgcctc atttacaaaa taatttttca taaactgtaa taattcacgg
     1801 tttgttgacc gcgttgaatt tgttcctact aatccaataa atttcatctt cattctcttt
     1861 cctttctaac atcgatttct gactgttcgc acgtactaaa ttttttcaaa tccttttgta
     1921 tacaagaatt gattctcttt tgttacaact gctccctcga tacccggctg ttcttcaagc
     1981 gctttcaaca cttctaaaga agatgctcca aataaacgtg tagtccagat ttctccatct
     2041 acagaagaat cagacaaaat cgtcaggctt gctacgtcat ttttaactgg aaagccagtt
     2101 ttcggatcaa tgatgtgatg atacactgac gaaccatttt gtagataacg ttcgtagatt
     2161 ccagaggtaa ctaaagattg gttgcttagt ttaaccaccg caagcgactc ttcacgtgaa
     2221 cctttaggat ttcgcaaacc aatacgccaa gcaccatccg aacgtcttgg tgattcacca
     2281 aaaacaacga tatttcctcc taagttaact aaagcaaacg ctgcatttcg ctctcggcaa
     2341 tgtgtaatta atttatctgc aatgtaacct tttgctaacg cccccaagtc aatcatcatt
     2401 cctggtttgg ttaaaaaaac ggttttcatt ttttcatcta attgaatttg gtgtggatcc
     2461 gttagcagaa gtaactcagt tatctctgct tttgttggaa ccaatgcatc ttcaaatcca
     2521 atgtgccatt tttgtacaac cggaccaata gcaatattca gataactatt tcttgcacaa
     2581 ctatgtgctt ttcctaattt gatgagctca aaaagttcag gatgtacaat cacagcttgt
     2641 cgtccagcag cttgattgat ggccattaat tcagaagttg catcatgtgc gctgaagcga
     2701 tgttcataaa cgtgcaacca ttcgcctaac tctgctagta atcgactagg ctcttcatgc
     2761 ccaacttgta atgtaattgt tgtccccatc atttgccaga ttttcgtttc ttcgatcacc
     2821 tgattcattt tgtaccttct ttacttgaac tacggattac atgagtaatt agtttttatt
     2881 tttgttactg ttttcacaac cagatgatac gcctgctaga tttatttgtc aaccgatttt
     2941 atatattttt ttatataata aggtaatttt aatataaaat tttatattgt aaaaaaacaa
     3001 ataagggaaa caaacaacac atttttgtgt gttgcttatt tccctctaac tatcgttttc
     3061 tatttgatgg ttaacctact taaaataaat actgcaacga caaacggaga tcaagcatgc
     3121 tttgtaacgt attttacgag aattacgact ttattttatt actctatctt gctgccatac
     3181 ccgtctatcc ctaatcatct ttggtgaatt ttttcaaagt tagcataaca aaaataagta
     3241 ttttttcctt cgttaatcaa tagtatcatt ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctttaatt ggttaagat
//
LOCUS       DACCDF010000132         4176 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCDF010000132 DACCDF010000000
VERSION     DACCDF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.140"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            612..1424
                     /locus_tag="IVK51_002652"
     CDS             612..1424
                     /locus_tag="IVK51_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432192.1"
                     /translation="MILLAVSMSLLIYTHILSTFMSVIVIGFLFIGSLFSKRKERKEM
                     SNLISNMIKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTKLQERALNISDSLIGAVN
                     NDLVTFTMGTIGVVSFILPLFFKKKFANKEKIINFCAVTTWFLSTNLFPWEFLQKTPI
                     QIIQFPWRILGFQVLFGSLILIIVFLKWKTSNKKSMCSLVGIVLLIFTVTVATEANYS
                     QKIQSYKGRLIMTKKDVTLYTTSRTGGLYDYAPLDALKYKDHLKKHEVKVYD"
     gene            3059..3613
                     /locus_tag="IVK51_002653"
     CDS             3059..3613
                     /locus_tag="IVK51_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6432193.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
     gene            3867..>4176
                     /locus_tag="IVK51_002654"
     CDS             3867..>4176
                     /locus_tag="IVK51_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432194.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     L"
BASE COUNT         1543 a          516 c          661 g         1456 t
ORIGIN      
        1 atggcctttt tacttttaca caattataag gactttatcg aaaaaaagca gttatttgtt
       61 aaaatcattg atattattaa taataagtat tttttgaaag gggaaatata aaaggttttt
      121 gaaaataaat aaaaaatagt attacttgtt ctattgctag ttattgctag tctttattct
      181 tttttgtttt ttagggatgt acaaaagatc tcaaccacag gagacattgc ttttcacttt
      241 tctagaataa aatgcttgtc ctcagtattt tctggtccaa taaattttac cacatttaat
      301 agctatggcg gaggcgtgaa tttttttatc catatctaac atctttccag ccgtaatatt
      361 ttactggaca acaaacaatt tgattctaag ttatatactt actatacgtt gggatactga
      421 atatctgtac aattctagtg agctatcaat atggacaaaa gaatagatgc agccttctta
      481 ttcagctgtt tgtatagctt ttatggattg atatatatta gcgtactgca atttcagaat
      541 caattgcgtt gacgattgta ccagttgtct tgtattacac atatactctt ctttatgaaa
      601 gaaaaaactg cgtgattctt ttagctgtca gtatgagttt gctcatctat acccatattc
      661 tctccacctt tatgagtgtg attgttattg gcttcttgtt tatcggctct cttttctcta
      721 agcggaaaga gagaaaagaa atgtcgaatc tgatatcaaa tatgataaaa gctgttggtt
      781 ggaccattct tttaacagct tacgtttggt atccgatgtt acaacaagtc ctacatcaaa
      841 aaattaatcg tccttttcgc acaaaacttc aagaaagggc attaaacatt tctgatagtt
      901 taatcggtgc agtaaataat gatttagtta catttacaat gggaacaatt ggtgtggttt
      961 catttattct tccacttttc tttaaaaaaa aatttgccaa taaagaaaag attattaatt
     1021 tttgtgcagt gaccacttgg ttcttatcaa cgaatctttt tccatgggaa tttttacaaa
     1081 aaacacctat tcaaatcatt cagtttcctt ggagaatctt gggttttcaa gtgctttttg
     1141 gttcattgat cttaattata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
     1201 gtagccttgt aggaatagtc cttcttattt ttacagtgac tgtagcaaca gaagcgaatt
     1261 attcgcagaa aattcaatcg tataaaggtc gtttaattat gaccaaaaaa gatgttacac
     1321 tttatacaac ctcgagaact ggaggtcttt atgactatgc accattagat gcattgaaat
     1381 ataaagatca tttaaagaaa catgaagtaa aagtctatga ttgatgggag aatgcatctc
     1441 ataaagcaca taattctaaa ataatctata cagtaggaga tgcgaatgag caaaatatca
     1501 cgttacctgt atttaattac tgggaacagt tatgaaagta aatgggaaaa agagcactat
     1561 agaaaatgac aatggtttag tgtctattaa aggtacggga aaaagaacta tagttgaggt
     1621 ttcatctacg tatccaacta cgatgattct atctttatta attagttcaa taacttttat
     1681 attcttattt acacgttatt taataagatg ctgcggttaa atgaaaatag attagataga
     1741 tattataaat atgttgattc tattttcata gcaatggaga aaatacatta gcaatatata
     1801 aaagaaggta tcaagagtca cggtgaattt attccgaaaa gtgaaaagaa caaatataaa
     1861 atttaataaa aatatagaac taaataggaa aacgtagacg taattttcta cgttttcctt
     1921 ttcatatata ttaaatatta cggaaattta tataaactaa tttagtgttt tgtttgtaat
     1981 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
     2041 tgctcatttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     2101 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     2161 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     2221 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     2281 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     2341 aagaactagt agaagaagga ataatggctt gataataaag tactacctat taaaggtagt
     2401 actttattta atttagtata aattaacata ctactatatt ttaagtttca tattatataa
     2461 attaataata tactaccatt ttttggtagt ataatttaaa ggtaataagt tattctttta
     2521 ttactatgtt tgggttttat agcaaggaaa gtttaataag agtttggacc tagcaaacaa
     2581 tattaatgag gtcattaaat cttattatct ttagtgatat aacgaaatat ctacacagac
     2641 aaaattatga tcttgcgtaa agttataaaa tattaataaa aatattttat taatgaaaag
     2701 cttggtagca cggttattca tatgctaatt tatttcactt tatagtaagg ggagttctta
     2761 gtatattgtc caaatttaaa tttcatataa tttaattatg taaattagct attctaagaa
     2821 cttctacgga taactgcccg tctaaaattt aattaagagc ttagaatctt catataagta
     2881 aggttaattt ttttgtacaa aatagtcgga atattctgag ttactgaaat gatataaaat
     2941 cataaactaa accccttgat tttcttcact tttaacagag tatatatata agttcatgat
     3001 tatggtaaaa tatatatata atcatgaact cagaatattt ttaagaaggg aaacaatagt
     3061 gagctataat gtacaaccgt taaggacaaa tgaagagata caagattttc agttttggct
     3121 aagaagaaca agtaatccag aaagagatag ttttttattt ttgtttggaa tcaataatgg
     3181 gttacgtatg tcagatatta taggattaaa agttggagac atacgaggta aaagtaagcc
     3241 tattattgtt gaacataaaa caggtaagcg aaaaccgatt tttatagata atttgagaga
     3301 agagatttta ttatatactg aaggtaaaga agaaaatgat tggttatttc caagtcggca
     3361 acaaggtcga catataacaa gggatcgtgt ctatcagata tatgcagata tagcagaaaa
     3421 attaggaaga gatgatatag gaacacatac attaagaaaa acttttgggt atcactacta
     3481 caagaaaact agagacattg ctaccttaat gtttattttt aatcatagct cgcaagcaat
     3541 tacaaaaagg tacattggta taactgaaga tgaaataggg gcatcgttac gtggttttaa
     3601 attgggaggc taattgagat attaggatat attttttgtg tataatgaaa acttagaagg
     3661 tgagattaat gaagataact gaattttcgg aagtatttaa aacgtttagt aacatttaca
     3721 atagaatacc agagatagaa caaaatgttg tagttgataa tgattattat atagagaatg
     3781 attttaataa accaagaaaa tatgacaacc tatacaattt tatacacaat agaacaacaa
     3841 taaataacaa cagtaacact cgagttttga ttaattcagc atattttcat attaaatatt
     3901 taaagaaata tgagtatgaa gaagttgtat tagattatgg gctaaataga cagtcatttt
     3961 taatttatgc aataaataac ggaaatttgc caagcaataa aaagaacata attattgaaa
     4021 catatgccta tctggagggg ggatctaaaa aactaattaa gtcatgtaat atagagtatc
     4081 aattatcagt aggagaaatt aaatgtgttt tcgaagaaga tttagaactt gacttaaaaa
     4141 aaatttttgc cagcattcat gattatatag gtttga
//
LOCUS       DACCDF010000133         4140 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCDF010000133 DACCDF010000000
VERSION     DACCDF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.141"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(39..305)
                     /locus_tag="IVK51_002655"
     CDS             complement(39..305)
                     /locus_tag="IVK51_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432195.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(316..507)
                     /locus_tag="IVK51_002656"
     CDS             complement(316..507)
                     /locus_tag="IVK51_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432196.1"
                     /translation="MSNIDNGREAIKGFMKANNISEYDLATAYGRSRTWIQRVLSGKD
                     KGPAVNAFILEVIRDHKIR"
     gene            801..1139
                     /locus_tag="IVK51_002657"
     CDS             801..1139
                     /locus_tag="IVK51_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432197.1"
                     /translation="MTVFERIKFLAKKHSKTMKQVTIDLGYSENYFYSLKSGKQPSAE
                     KLTEIANYFNVSVDYLLGRTDNSEDSDSQLPPTDLDDALDGAMTWNGKPLTDQDKEAV
                     RIFLQGRKSE"
     gene            1151..1564
                     /locus_tag="IVK51_002658"
     CDS             1151..1564
                     /locus_tag="IVK51_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6432198.1"
                     /translation="MDNIEDLLKENGIDVEITNIESEGFYLPKLRTIFINQNLDELEQ
                     KKVLLHESRHALSHNELISLYSKTVFHSKMEDEANRFMIEVLLQDYMNLFALSVDQIN
                     YMKFMDYYGIGYDCEEYIKKLLVNYVTSSMYLNVI"
     gene            1578..1778
                     /locus_tag="IVK51_002659"
     CDS             1578..1778
                     /locus_tag="IVK51_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432199.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRAVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            1865..2752
                     /locus_tag="IVK51_002660"
     CDS             1865..2752
                     /locus_tag="IVK51_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6432200.1"
                     /translation="MKKMIGSLLVLASLLLFTACGKKVTTDDLKANDWLIEATKDDEP
                     NMIASFSDHIVSLSVDTSKMKSTASDEWDAMGEEFAKSLVDQMNYKFEYTLEGNVMTW
                     KDDKDKDNDAKYTVSKEDKNIILTPEKSNKSDDKEKLVLKPYKKEKTKDSRTTTSSTS
                     SNEQSSSTTTESTIASSSTVEQAAIAQNTPPETSSTYSAESVENYAEATPSSSVEQVS
                     STPSSTEQSVPQDYVPANQAQPTAPSSSETETLTLQPGEGPKQIAERAGITVDQLFAL
                     NGMDPNNYMLYPGQELRVK"
     gene            2890..4095
                     /locus_tag="IVK51_002661"
     CDS             2890..4095
                     /locus_tag="IVK51_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP6432201.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIEFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQSENLFFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
BASE COUNT         1535 a          702 c          695 g         1208 t
ORIGIN      
        1 actaatgttc gattaaactt tcccatgttt ttcctccttt aaattttgta tttagccatc
       61 agttcatcga tgtccttttt gtcatatctc aaaaagccat ctatacgaat ttcttttaaa
      121 ccgtgagcta ttaacttctc aaagccttga tcgtttacac ctcccacata ttttctagct
      181 tcgatctttt ttaaatatcg tgtttctgac aaattctttt ttgaaagttg actaagtgcg
      241 tcctttacaa cttcaacaat tgcgattctc aatcgagtca gaaactcttc gcttagaata
      301 ttcatgactt gcctcctatc gaattttatg atcgcgaata acttccagaa taaaggcgtt
      361 aacagctgga cctttatctt ttccacttaa aacacgctga atccaagttc tcgatctacc
      421 atatgcagtg gccaaatcgt attctgaaat attatttgct ttcataaatc ctttgatggc
      481 ttcccgccca ttatcgatat tactcacttc acacacttcc tttcttttta tttagaaaga
      541 aaattggata gaaaagtata tttttagttg actacgaact atactatagt gtagtatata
      601 gccatagtta aataagccta taacaaacct ttttttatcg cactcggtcg ccaaacttaa
      661 tgctataagg tgtgttttta ctttgctttt tttctatcca attaacttac aaaaccaata
      721 tacactatag tgttgtttag gtcaacacaa aactacactt ttttattggt tttttgtaaa
      781 gaaaaaagga gaatgctggt atgacagtat ttgagcggat aaaatttctt gcaaaaaaac
      841 attcaaaaac aatgaaacaa gtaacaattg atttaggata cagtgaaaac tatttctata
      901 gtttaaaaag tggaaaacaa ccatccgctg aaaagcttac tgaaattgct aattatttca
      961 atgtatctgt tgattatcta ctaggtagaa ccgataactc ggaggatagt gattctcaat
     1021 taccaccaac cgatttggat gatgctttag atggagcaat gacttggaac ggaaaaccac
     1081 ttaccgatca agataaagaa gcggtaagga tatttttaca aggtagaaaa agtgagtgag
     1141 gttgtagtgt atggataaca ttgaagattt gttgaaggaa aatgggattg atgtagaaat
     1201 taccaatatt gaatcagaag gattttatct tcctaaatta aggacaattt tcattaatca
     1261 aaatttagat gaattagagc aaaaaaaagt attgctacat gaatcacgtc atgcactaag
     1321 tcacaatgaa ttgatatcac tttacagcaa aactgtattc cactcaaaaa tggaagatga
     1381 agcaaataga ttcatgattg aagtactgtt gcaagattat atgaatttat ttgctttatc
     1441 tgtagatcaa atcaactata tgaaattcat ggattattat ggaattggtt atgattgtga
     1501 agaatatata aaaaaattac ttgtgaacta tgtcacttct tctatgtacc ttaatgtaat
     1561 ttaaatggag gcttcttatg aaaataggaa tgagaaaacc gagtataaaa aaatccatta
     1621 gcgctagaac tactggaaag gcaaaaagag ctgtgaaaaa agcagtgatt cctggttacg
     1681 gtaaaaaggg atctggttgg atcaaagatc caaaaaaggc tgcttacaat aaagtataca
     1741 agaaaactac attcagtttt tgggatttat ttaaataaaa aaagcccgtg cagcaacacg
     1801 gactcgaaac cttatttcta agattcccca ataaaaatca tatcatagaa atgaggaata
     1861 aaaaatgaaa aaaatgatag gaagtctgtt agttcttgct tccctactac tcttcactgc
     1921 ttgtgggaaa aaagtaacca cagatgattt aaaagctaat gattggttga ttgaagcaac
     1981 caaagatgac gaaccaaata tgatagcatc attttctgat catatcgttt ctcttagtgt
     2041 tgacacaagc aaaatgaaat ctacagctag cgatgagtgg gacgccatgg gagaagaatt
     2101 tgctaaaagc ctagttgatc aaatgaatta taaatttgaa tacactcttg aaggtaatgt
     2161 tatgacttgg aaagacgaca aagacaagga taacgatgcc aagtataccg ttagcaaaga
     2221 agacaaaaac attattttga ctccagaaaa atctaataaa tctgatgata aagagaaatt
     2281 agttttaaaa ccctacaaaa aggaaaaaac taaagactct agaacaacta ctagctcaac
     2341 aagtagcaac gaacaatcat cttcgactac aacagaaagt actattgcat cttctagtac
     2401 tgtagaacaa gctgctattg cgcagaatac gcctcctgaa actagtagta cttactctgc
     2461 tgaaagtgta gaaaactatg cagaagctac tcctagttca agtgttgaac aagtttcatc
     2521 cactcccagt tcaacggagc aatcagtacc acaagattat gtaccagcta atcaagctca
     2581 accaacggca ccttcttcat ctgaaaccga aaccttgact ttgcaacctg gtgaagggcc
     2641 caaacaaata gctgaacgag ctggaattac ggtagatcaa ctgtttgcat taaacggtat
     2701 ggatccaaac aactacatgc tttatccagg acaagaacta agagtgaaat aaaaaaagca
     2761 catttgccgc ccgaccaaga acgcaaatgt gcaaagagct acggtaaagt aggtctattt
     2821 gttgtaccct attttaccat ctcgaaatcc gctgtacaac cgaacaaacg tacgaaagga
     2881 aagataaaga tggcaaaaaa agaaattgat aaacgaatta aaaagcactt aacaaaaaaa
     2941 ggcgaagaaa aatatcagtt tagtctgtac ttaggagttg atcctctaac aggaaaaaaa
     3001 agaaaaacaa cgagacgagg ttttagtacg ccattagccg ctgagagagc tttaaaaaga
     3061 ttggaggcag atatacagga aaaaggactg caagcttcta cagctcaaaa gagcaagaaa
     3121 ttcgaatatg tttacggctt atggtttgaa aattataaaa agacagttaa agaaagcact
     3181 tggtcctcca ccaaacaaat ttttgacaca cacatcttaa aagtgtttgg agataagttc
     3241 attgacaaaa tagacgtttt tttctgtcaa gaagcagtaa acacttggtc cgatagccat
     3301 cctaaaattt ttaagaaaat aaaaaattac acatctaatg tttttgatta cgctgcttct
     3361 ttacaaatta taactagcaa tcctatgaaa ttagtttcta ttccacgtgg agaagctcta
     3421 gatatcgaag ataaaaatat cgaattttat actaaagaag aattaatcga atttttagaa
     3481 gcaataagaa acgatgatga tgaaagatat ttattctttt ccctattggc ttttacaggt
     3541 ataagaaaag gagaagcttt tgcactgacc tggtcggata ttgattttaa gagcaaaaca
     3601 ctcaatatta ataaaactgt aacaagaggt tatcaaggga gattgatcgt aaatactcct
     3661 aaatcaaaat ctggaaaaag aaaaatttat ctggacaatg atttgatcag tttattgaga
     3721 aagtattata caaaaaacaa aacgatcgtt acaatccaaa gcgaaaactt attttttcac
     3781 catgatggtc ttctctataa ccctacagtt tcacgttcgt ggcttaatgt catatataag
     3841 catcatccag aattaactaa aagaatcact actcatggtt ttcgacatac tcatgcctct
     3901 ctcctttttg aatcaggagc ttctttaaaa gatgttcagg aacgtttagg gcatgcagac
     3961 attcaaacta cttcaaatat ttacactcat gtaactgaaa ctcaaaacaa aaaagtcatc
     4021 aataattttg tagcatttat gaagaatagt actcaggggg agtcaaaagg ggagtcaaaa
     4081 aacattttag gctaaattac aacaaaaaaa gacacgatcg aaatcgtgtc taatcgttga
//
LOCUS       DACCDF010000134         4035 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCDF010000134 DACCDF010000000
VERSION     DACCDF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.142"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            178..774
                     /locus_tag="IVK51_002662"
     CDS             178..774
                     /locus_tag="IVK51_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP6432202.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            898..2697
                     /locus_tag="IVK51_002663"
     CDS             898..2697
                     /locus_tag="IVK51_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP6432203.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2975..3187)
                     /locus_tag="IVK51_002664"
     CDS             complement(2975..3187)
                     /locus_tag="IVK51_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432204.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT         1185 a          688 c          712 g         1450 t
ORIGIN      
        1 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
       61 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
      121 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
      181 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
      241 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
      301 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
      361 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
      421 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
      481 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
      541 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
      601 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
      661 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
      721 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
      781 ttttgtaaaa ttagtcattg attgacaatg ttaaatctgg gaaagggtct gcaaaacaca
      841 ctgcaccctt tcctttttca ttagatttgc gctacaatgt gaagagcagg tgataggatg
      901 aattatttga aacgtagctt aggtaatgct tgggactttt taaaaggaac acaagcttat
      961 ttccgtgatg tcttgctgat gcatggcttt attttattta tttgtttacc cttattgagc
     1021 agtacgaccc gttttatttt acaccgaggg gcaattgact atttgtctta tgacaatatt
     1081 ccttcaattt tttctcaaca tcctggggtt ctactctcgt taatcgtagt tttgatgacg
     1141 attttactgt tagtttattt cgaatttact tttcttttga tgagtgtttt ttttattaaa
     1201 aagaaagaac caatctcttt gaagcaactc ttacacttga cgatacttca gctgaagaaa
     1261 gtgagaccca ttactttttt gtttttcctt gcttattttt tattgattct accgatcagc
     1321 ggattaagtt ttaattctga tctactgtca aaaataaaaa tccccgcttt cattatggat
     1381 tttatttttg ctaatcgttg gatcatcgtc agttcctttt tactcgttta tattttcctt
     1441 ggctatattg ggattcgact gatttttgct ttaccggaaa tgattctacg ggatcgtcct
     1501 tttcgagcag ccattcggga aagctggtca ttgaccaaat cgcgtctttt ggccatcacg
     1561 ggccaattta ttgtgattgg cggtacgatt ttactccttt cttctttagg atatatcgtt
     1621 gttattttgg cacaaagtat ggttgaacaa tttttcccag actatgcatt gatcagcgct
     1681 gtgtttgcta tgacgctttt acaagggatt ttattattca atatcgtgat gtctaccgta
     1741 gggatttttt atatcattgt tgatttcatg gatgacgaag gcttcttgcc tgagatccct
     1801 aagtggttta tcccacaagc ccccaacttg cgtttctctg cgctgaaaaa cactggtttg
     1861 actctttttg cagttttttt cggtattggc gtctgtcttt acaatatgga ttatctgact
     1921 tcagctgttc agacaaaacc agtgactgtc tctcacagag gtgtgagtac ccaaaatggg
     1981 gcgcaaaata ctttagaagc acttgaaaaa acaagtagag actatcatcc tgattatgta
     2041 gagatggatg ttcaggaaac aaaagatggt catttcgtag tgatgcatga tgcaaatctt
     2101 cgtcacttaa caggggtaaa tggaacacca caggatctta cattgaaaga attgacaaat
     2161 cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt cttttgattc ttatttggaa
     2221 aaagcacata agctaaacca aaaattactt attgagatca agacctctaa aaaagattcg
     2281 gatgaccttg tcgaacgatt tgtccataaa taccgcaaag ttatcttaga gaaccatcat
     2341 attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa aagaagcaga accaaacttt
     2401 tatgtgggct acatcttacc tttcaatatt gttgggcccc ctgtgacgcc tgctgatttt
     2461 ctgacaatgg aatatagtac gatcaaccgt aacttcatta attctgccca ccaagacgga
     2521 aaagaagtat atgtatggac aatcaacgat tcagatgcta tgagtcgcat gatgttttat
     2581 ggtgtggatg gtatcataac cgatgagatg ggactactaa atgaaagtgt gaaacaaaca
     2641 gatgaagaaa tcacttattc agataaactg ctaaattttg ttttgggagt cggctaatca
     2701 aaaatcgtta atgaaaaaag tctttataca acaaaaaacg cataacctgc caccgcaggt
     2761 atatgcgttt tttgtttgac gctcataatg tgtgcttaac tttctcttga ttccttcatc
     2821 gcacgaacaa tagctaaaat agcaggaatt gcagagatca ctgcaagaat aattcccatt
     2881 ttccctgaac tttttctttt tggcatatct tctcgctccc ttctgtcttt atttatatta
     2941 taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga aaccaattca gcaggtgcgc
     3001 taatatcaca gaattttacg agcatgcgat acttgctttc attgataagt tgatagtctc
     3061 gttggtcata actttcaaca tacacgacag ccgtgttgat atatgtacgt tcaatacagc
     3121 cgattagttc atgagatact ccgatcggtt tgcatatata agatcttcct acttctgctt
     3181 ttaccatttc attccctctt ttctttattt ttattttgtt ttttctttat tttataataa
     3241 caaattacct tgtatttgaa aacgttttta tccatttttt tattttttat tttgagtatc
     3301 tttctgcttt ttagataact attttttaac acgatcttag acgttgtgac aaaaacgttt
     3361 gtttatattc ctatgttgtt cgtgcgtttt tttctttata ttgctaagtt atttataaag
     3421 aggtgagcat atgatttata ttcaagcaat agtatttgta accttgatga ttgggctact
     3481 tatcggatta aaagaaaata gctaaaccat ttaagaaaaa ataaataagg aaaatgataa
     3541 atgcttttta attgcatcaa aaaacgtacg atgctaaact tggtataagt tcgaaagttt
     3601 aaaggaaact ttcaagaact aaattacatt acaaaggaga tgaattttat gacgtttaca
     3661 aaaacaaata tgcaataccg cgtagctttt gatacaactt ctaaccgttt tatggcgatt
     3721 gatgcacaaa acgaaaataa cgttgcgttt ggtattacaa tcgaacaagc agtccgagca
     3781 ttaaacgaat ctagataagg agtgaaccat tccaaatggc gactgatgag atctcggaaa
     3841 atcaataaaa gacgtttgta acggtaatcc gcgacaaacg tctttttgta ttcattacat
     3901 atatttggaa attgtctctc tttatctcta gacctaaaca aaaacacaag ttctaatatc
     3961 aaccatctcg ttttttatga caattttgtt agctgtttcg atgataatat tccgttatta
     4021 tctgtataat attta
//
LOCUS       DACCDF010000135         4025 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCDF010000135 DACCDF010000000
VERSION     DACCDF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.143"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..185)
                     /locus_tag="IVK51_002665"
     CDS             complement(<1..185)
                     /locus_tag="IVK51_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6432205.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQ"
     gene            complement(1022..2107)
                     /locus_tag="IVK51_002666"
     CDS             complement(1022..2107)
                     /locus_tag="IVK51_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP6432206.1"
                     /translation="MSTENQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHKAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFIEK
                     AIAAKLANEQRETFNELPTVSTENWLE"
     gene            complement(2427..2834)
                     /locus_tag="IVK51_002667"
     CDS             complement(2427..2834)
                     /locus_tag="IVK51_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432207.1"
                     /translation="MPKDFDIPKMMENGEFEPLLPNNKRQDVKISGEYNFSNSALSNT
                     RNITLKKRGRPVSIPDKRNKVSVPKKISPALNSKLSILQDYMTELQSEKGRISFEKIV
                     NTLADSYIQHRLGVAKEEHIREEIQEEFEKLPK"
     gene            complement(2853..3671)
                     /locus_tag="IVK51_002668"
     CDS             complement(2853..3671)
                     /locus_tag="IVK51_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6432208.1"
                     /translation="MDTFQGTTFTVGNFKGGVGKTKIVSMLAYDNAMIRNRKTLIIDL
                     DPQANLTQVIARTFGITDIKTTITNGVANGELASSIIKVNENLDLLACDTSFRSFSAF
                     ANKHENELDQVTVLKKLLLPIKNNYQEIFIDVPPTISEFSDNAMAASDYSIISFQTAE
                     ESLEGVNKYVNYQKFMVDRYDLDLQIIDIVPCMVEPNDDFDEEILEEAIEKYGTVVSK
                     NLIHYQKRLRRYSKIGIHLRKYKNGNFDQWDYKAHQVFINILAELDARQEFLNK"
BASE COUNT         1232 a          648 c          557 g         1588 t
ORIGIN      
        1 ttttgtttcc caatgtaata aatcatttct tctgagactt tgtctttctg gattatatat
       61 ttcttacact gcacaacggt tttactttga ccagttgata gtttcttcgt gatcttttct
      121 gtagcggtac accgtttacc gtagtgacaa atatcttcga gactcaaaat tcctgtatat
      181 gacataaaat aatccaccca cttactttaa atatttttaa caagtttttc ttgttatttt
      241 tcaaaaatag agaattgatt ttcgttcatg acaaacaaaa aagagggaac gcattcaaac
      301 atgcgttccc tctaatttca gcgttcgtca tacggcaaag agccccacgg acatttttat
      361 aacattatgc ttaggcatag attcatcgta atatcacgta tttttaacct taacacagcc
      421 aaaaaaagtg gtatcacttt tgaatatctg ttatactaaa tccgttacgg gtaataccgt
      481 aacactgaaa ggtaaacgca ttaaggggta tagacttaat gccacctgtg gacgtcgacg
      541 aaggttggta gccttccgag tctcacaggg cctttctttt ttttgtattt tcaattgtcc
      601 aaatttatcg tccaaaagtg cagaacgaaa atcaattcat cacaagcata gcagacaatt
      661 aaatttaaaa caactaaatt tatacaaaaa agtgtaattg aatattagca gcactgcctg
      721 aaaagaagta atatcaggct tttcagcttg ggtaaataag tgaattacct ctcttcatga
      781 attattataa ctcaaaaaaa tacatatgca aacactagtt ccctgttttt ttgaatgaaa
      841 aataactaaa atatctaacg attatgacat taaaacctac gtttgtgatt gttttcataa
      901 acttttaatc taaaaactcc ggaataataa ctatttattt gtaacatttg taataaaatt
      961 aaaattgtaa aaaagttcta atttaaaatt gatcattttt atagtgttcg ctttttatca
     1021 actactctag ccaattttcc gtactaactg ttggtaattc gttaaatgtt tctctttgtt
     1081 cgtttgcaag ctttgccgca atagcttttt caataaacca gttttttaca tacacaaaaa
     1141 tcaaattctg caaattattt actttctcag tcttttgttt ttgataagct ttcagcaacg
     1201 tcatatataa tcctttatcc gcatcaattc cgtaagattc aagttcttca tacacaacta
     1261 ccgtcttctc aagttcctcg gctttatgtt ttgctttgtg tattgtggtg acggtttgtt
     1321 gggcttcttc gtaagtgtta gaaaatgttt ttatcaagct taataccttt tctggtacaa
     1381 atgtgtttat gctgtcttct ttcatgattt caacgaagtt atctagcaaa acttgatttt
     1441 gtaattggtc tgtttcagtg tcttttaagt gtcttttagt gtctaaagat ttattaatat
     1501 aataatcttg tgcgatttcc gcatttccag tgttatcaag ggtttcagct gttttttctc
     1561 gacgtgcgat ttccgcattt ccagtgttat caagggtttt agcagtttcc tctcgacgtg
     1621 cgatttccgc attccgagaa gaggttgatt tatcaatagt ttcgcttaaa tcgtcatcga
     1681 aagaattttg aataaaaaga tcatcggcac ttatttcagg ttgaagtaaa tagtatttat
     1741 ttttttcatt ttttttcgtt tttgggttaa attctccttt tacgactgaa atcaaattat
     1801 atgtttctaa ctcgtttaaa atggttttta ctttgccttg gtagcaattt aatttgtcca
     1861 ttaggtctgc ttgtgtaaat tccaaatata atagaccatc atcgtcgatc catttttcat
     1921 gtaaggctct acgaaattca tttttaaaaa gcatataggc tatttttgag ctgtctttca
     1981 ttttagagta tttagaattc tgaattaaaa aactaggaaa tttataatat ttttcaatat
     2041 aaacattcgt tgctttatat ccccaatttt taattatcat tttttgagat tgattttctg
     2101 tagacattaa atttctcctt cttcttagaa acttgtatat attcaggatt ttcaacaagt
     2161 gaactatacc atgtattaag ggtgaataaa acactttcag tatttaaggt ataagttata
     2221 tattaaaaaa ataagtataa gttatacttt aaatacaaat aatgtataac ttatacttta
     2281 aataataatt ttataatgtt gatttaatcg caaatttaca cgttgtttat taagttatac
     2341 cttaaatatt taatgtataa cttatacttt aatattcatt tatctagtat aacttatact
     2401 ttaattgaat accgcatgaa aaaaaattat tttggtaatt tttcaaactc ttcttgtatt
     2461 tcttctctta tgtgttcttc ttttgctact cctagtctat gctggatata tgagtctgct
     2521 aatgtattaa caattttttc gaaagaaatt ctcccttttt cactttgtaa ctccgtcata
     2581 tagtcttgta aaattgagag tttgctattt aatgccggag agatcttttt aggaactgat
     2641 accttgtttc ttttatcagg aattgaaact ggtcggcctc tttttttcaa tgtaatattt
     2701 cttgtgttac tcaatgcact attgctaaag ttatattcac cgcttatttt cacatcttgg
     2761 cgtttgttat tgggtaataa tggctcaaat tcaccattct ccatcatttt aggaatatca
     2821 aaatctttag gcattactta tgcaactcct tattatttat ttaaaaactc ttgtctagca
     2881 tctagttctg ccaaaatatt tataaatact tggtgtgctt tataatccca ttgatcaaaa
     2941 tttccatttt tgtattttct taaatgtata ccaattttag aataccttct caatcttttt
     3001 tgataatgaa tgagattttt ggataccacg gttccgtatt tttcaattgc ttcttctagg
     3061 atttcttcat caaaatcatc atttggttct accatacaag gtacaatatc tataatttgc
     3121 aaatcaagat cgtatctatc gaccatgaat ttttgataat ttacatattt gttgactccc
     3181 tctaaggatt cttcagctgt ttgaaatgaa attatcgaat agtctgaagc tgccatagca
     3241 ttatcggaaa attcgctaat ggttggtggt acatcaataa atatttcttg ataattgttc
     3301 tttattggaa gaagtaattt tttcaagaca gtaacttggt ctaattcatt ttcatgttta
     3361 ttagcaaaag cggagaatga tcgaaaacta gtgtcacatg ccagaaggtc aagattttca
     3421 tttaccttaa taatactact tgctaattct ccatttgcga caccatttgt tatagtggtt
     3481 ttgatatcgg ttataccgaa agttcttgct atgacttgtg ttaagttcgc ttgagggtct
     3541 aaatcaataa taagcgtttt tctatttctt atcattgcat tgtcataagc aagcatgctg
     3601 actatcttag ttttacctac tcctccttta aaattgccaa ctgtaaatgt agtcccttga
     3661 aaagtatcca tgtaaatcct cccaatcttt aattgataat taaagtataa cttatatatc
     3721 ggaaatggtc aacattaaaa gtataactta aataataata tctaaaaacg tttggatata
     3781 gcggttttta ataataaaag tataagttat accatatata tttatggtat aacttatact
     3841 tttattatta aaggtataac ttatacctta aatattgctt ttcgaaaata gctattctag
     3901 tctatctgtt tctttacttt tcgctgctga tttgaattga attgatcata ttcattttct
     3961 agtattaata acttttcgta tacatcggtc atattcatac tagaagcaag acttagatca
     4021 tatac
//
LOCUS       DACCDF010000136         3956 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCDF010000136 DACCDF010000000
VERSION     DACCDF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.144"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..156
                     /locus_tag="IVK51_002669"
     CDS             <1..156
                     /locus_tag="IVK51_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP6432209.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVK51_002670"
     CDS             175..1452
                     /locus_tag="IVK51_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6432210.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVK51_002671"
     CDS             1462..2118
                     /locus_tag="IVK51_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP6432211.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVK51_002672"
     CDS             2118..3494
                     /locus_tag="IVK51_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP6432212.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERTKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IVK51_002673"
     CDS             3624..>3956
                     /locus_tag="IVK51_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432213.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1595 a          423 c          692 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagaaca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACCDF010000137         3923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCDF010000137 DACCDF010000000
VERSION     DACCDF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.145"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(573..1130)
                     /locus_tag="IVK51_002674"
     CDS             complement(573..1130)
                     /locus_tag="IVK51_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432214.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVK51_002675"
     CDS             complement(1309..1920)
                     /locus_tag="IVK51_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432215.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVK51_002676"
     CDS             complement(2033..2290)
                     /locus_tag="IVK51_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432216.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVK51_002677"
     CDS             complement(2887..3165)
                     /locus_tag="IVK51_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432217.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVK51_002678"
     CDS             complement(3199..3450)
                     /locus_tag="IVK51_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432218.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACCDF010000138         3883 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCDF010000138 DACCDF010000000
VERSION     DACCDF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.146"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..3123
                     /locus_tag="IVK51_002679"
     CDS             82..3123
                     /locus_tag="IVK51_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP6432219.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3313..>3883
                     /locus_tag="IVK51_002680"
     CDS             3313..>3883
                     /locus_tag="IVK51_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP6432220.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1329 a          621 c          824 g         1109 t
ORIGIN      
        1 atagctgaat tgatggaact tgagagttat aaaaaagctc tcatctacga atacgtaacc
       61 ggtaaaaaag aagcctagga ggtgattcat ttggtagaga acaaagaaat tcgttttgag
      121 caggatattg agacttcact gactacgtat ggcaaatgga agaaagaaac attcaatgaa
      181 tcaaactttg atcccataat tggtttggat tgtcaaaaat tgctaggctt tgtccagaag
      241 actcaaccaa aacagtgggc acgtcaccaa cgaaactata acaatgatgg agaagtcaag
      301 ttccttcaac gctttaatca agaggtagaa acccacggca tgttacatgt attgcgcaat
      361 gggatcaaag accgcggtgt gcgctttaaa gtagcttact tcaaacctgc ttcagagctg
      421 aatgatgaaa cgatggaaaa gtatgaagca aatagtttta cctgtactcg ccagtttgct
      481 tacagtccta ataaccataa aaccattgat atggttttgt cgttaaatgg tattccgctc
      541 gttgctttag aattgaaaaa ccagtacact ggtcaaacag tagaaaatgc caaactccag
      601 tttcaaaaag atcgtgatcc aaaagaattg gtatttcgtt ttaatcgccg cttcttagtc
      661 tattttgcgg tggaccatac agatgtttat atgacgacaa agttggacgg taaaaaaacg
      721 tacttcttgc cattcaatca aggttccaat gggccgggaa gagttggtgg agcaggcaat
      781 ccgtccaata aagatggcta cccaactgcc tacttgtggg aaaaggtgct tactagagac
      841 aatttgatgg atattatcca acgttttatg aatctagaga ttaaaaaaga aaagcaaggc
      901 gacagagaag tcacaaagaa aacgttgatt tttcctcgtt atcatcagtt agatgtggtt
      961 cgaaaattgg ttgcagatgt aaaaagccat ggtagtggag aaaattattt gatccagcat
     1021 agtgctggtt ctggaaaatc taatagtatt gcatggctgg cataccattt agcctctatt
     1081 catgatcaac tgaactaccc aattttttca tcaattatta ttgtgactga tcgaacagtg
     1141 ttggatcgcc agttgcaaaa tactatcatg aactttgatc atcagacagg tttggtagaa
     1201 acgattgacg atcgcaaatc ctctcaagat ttaaaagaag ccattaatga tgggcgtaag
     1261 atcattatta ccactttgca aaagttccca gtgatctatc aggaagtaga tcaagtggaa
     1321 ggtcgtaatt ttgctgtgat tgtggatgag gcacattctt cacaaactgg gaacagtgcc
     1381 cagaagctaa aagccgctct ttcggacaaa tctaaatcct tggaagagta ttatgaactg
     1441 gaaggcgaag ttgacgatgt attaccagat gctgaagatg aactagttca acaattggta
     1501 gcacaaggta aacatcaaaa tttaagtttc tttgctttta ccgctacacc gaaagaaaag
     1561 acattggaaa tgtttggtac gaaacaacca gatgggcatt tcgaaccctt tcatgtttac
     1621 tcgatgcgcc aggcgattga ggaaggcttt atcttagatg tgctgaagaa ctatatgacc
     1681 tatagtactt cctacaaaat tgcccgtgaa attgaagata acccagaatt accaaagagt
     1741 gaagcaaccc gagcgattgt tcgtttccaa tcattgcatc cttgggcagt tactcaaaag
     1801 acggctgtga tggttgagca attccgagcg gttactcgta cagctattgg tgggcaggca
     1861 aaagcaatgg tcgttaccgc atcacgctta cacgctgttc gctatatgaa agaatttaaa
     1921 aaatatatag cgaacaaagg ttataatgac ttggatgtgt tggttgcttt ttctggttcg
     1981 gtagtagatg gagagactga atatacagaa acgaagatga atgtcacaaa atctggtgag
     2041 aatgttcggg agaatcaatt aaaggaagct ttccatacag ataattttaa tatcttgatt
     2101 gttgctgaaa aatatcaaac gggttttgat gaaccgttgc tgcataccat gtttgtcgat
     2161 aagaagttaa agggtgttaa agctgttcag actttatcac gactcaatcg aacaatgcct
     2221 gggaagaagg atacctttat tcttgatttt gtaaatgatg ttgaagatat tgaagctgct
     2281 ttccaaccct tttatgaaca aacaaatttg agagaagaaa tcaatgttaa cttgatttat
     2341 gatactcaaa gcaagctacg aaaatttaat gtttacaatc aaggtgatat tgaacgtgtc
     2401 atgaaattgg tgaaacaagc ccaaaagcaa cagaatgagc gcttgcttgg acgaatttct
     2461 agcatgttta agccgattat ggatcgctat gaggaaatga acaaggataa tcagtaccag
     2521 tttcgggtga ctctacgtaa ctttggcaag tggtatgatt atatttcaca acttgatcgg
     2581 ttattcgata tggaactatt ggaggaacga atttttactg gatacttgct gaagtttcta
     2641 cctaaagaaa cacgagaaaa tattgatatt tctgacaaag taaagttgga atattataag
     2701 ttacgtcagg acttttccgg tgaaatccat ctagtgaata aagaaggcga gctagtaaat
     2761 ccaaagtcaa ttgatgcagg aatcaaaccg ccggatgaac gagattcact agaagaaatc
     2821 attcataaaa tcaatgaacg atttccaaat gattttgacg aaggagaccg ggtgttagta
     2881 gagattttgt atcggtcatt tgccgataac ccagatttga aggttgtcag catggctaag
     2941 aataacgatg ctcagatgtt tgaaaaatcg ctgttccctg atgtatttaa agataaggtg
     3001 atggagcagt acgagaagaa caatgcagct tttgagaaaa tgtttgggca tgatgataag
     3061 tactttgatc tagtatatac attagttgct agagatttat acaagttatt acggacgaaa
     3121 taaaatttat aaataaggat tagacaatac aatttctctc aaaaataaag gagaagttgt
     3181 attgtttgct tttaagaaag ccctgaaaaa tttgttacgt actaaattgt ctgaactctg
     3241 tcatatcagc tatgtcagaa taaaggaaat agatttattt ttataaacag tgtcgtctcg
     3301 gagatttaaa ctatgtcaac gacaattgaa gtgaacaaac aaagcgtgaa gcagtttctt
     3361 gaaacaggga aaatcaaaaa attcgttatt ccagaatatc aacgtcctta tgcctggact
     3421 gacgagcaaa accaagtact atttgatgat ttagcggagt acacagcaaa caataacgag
     3481 agtacttact ttttaggaag cattgtggct tatgaaaatg atcataacga gcaagaaatc
     3541 attgatggac agcaaaggat aactacgcta tttttatttt tgagagctat ctatgctaaa
     3601 ttagaaaata gttgtgaaaa ggaagcttta ttcttgaaaa gccaaattga acccgcgtta
     3661 tgggaacaag atgatttaac aggtgaggtt aaaccagata aaattctgat tatgtctcga
     3721 gtaatggggg atgaggggaa tgaagaattt gctagtattc tagtaagtgg tgaagcagat
     3781 gttaaatcaa aaagtaatta ttcgaagaac tatattttaa ttcaacatct attaaatgag
     3841 tatgcaacaa acgaaccact atcattttat cgttttatca gta
//
LOCUS       DACCDF010000139         3810 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCDF010000139 DACCDF010000000
VERSION     DACCDF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.147"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            94..2193
                     /locus_tag="IVK51_002681"
     CDS             94..2193
                     /locus_tag="IVK51_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP6432221.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2389..3252
                     /locus_tag="IVK51_002682"
     CDS             2389..3252
                     /locus_tag="IVK51_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP6432222.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3357..>3810
                     /locus_tag="IVK51_002683"
     CDS             3357..>3810
                     /locus_tag="IVK51_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP6432223.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1524 a          636 c          772 g          878 t
ORIGIN      
        1 agtaaatcct aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag
       61 cgtaaaccta gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg
      121 aaaaaaatgg agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat
      181 cagcaaataa aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca
      241 actgaagatg cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg
      301 aaagatttac ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa
      361 gaaccacctg ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact
      421 gttcatgaga cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa
      481 aaaagaagag tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta
      541 tccgcttata tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc
      601 aaaatgatca gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa
      661 actgaaaaag actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac
      721 gtaaaaacgg atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa
      781 aaaacgaata aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag
      841 tcaacagagc ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa
      901 gagggtactg atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa
      961 gcagaagcga aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac
     1021 ttagaaaaaa cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc
     1081 gttttagcag cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa
     1141 aaagtagaag ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat
     1201 gagaaacttg ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt
     1261 catcaagatt tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat
     1321 tcagagttag aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg
     1381 aatgagtggt tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg
     1441 aaagcagata aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt
     1501 caacaagcac aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa
     1561 acagctatag tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag
     1621 acggcaaaag aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa
     1681 caattgaaac aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag
     1741 aaaaaagcag aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca
     1801 gcagctgcag ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg
     1861 gcatcctcga ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca
     1921 agtgatattg ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa
     1981 gtgattgcta catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa
     2041 gccagaatcg aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca
     2101 ttgatgaaag gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa
     2161 actggatggt tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag
     2221 cctgccgttt aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc
     2281 cttttttcac ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat
     2341 agcaaagaaa tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta
     2401 ttagcaacta tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa
     2461 aaaaacggac aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca
     2521 gttgaaggat tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct
     2581 aaaagccgta tcggtcatta cgcttttgga atcgttactg ctacacgaaa agatttaggt
     2641 gcgtttgtcg atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg
     2701 acaatgacag agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat
     2761 gcaaaagacc gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa
     2821 tatggaacag aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta
     2881 acaggtacgt acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga
     2941 tataatgaac ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac
     3001 ggtgtactga atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa
     3061 atgattttaa ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg
     3121 gatccagaag aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta
     3181 ggtaatcttt tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa
     3241 aaatctaatt gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa
     3301 catagtgtta tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg
     3361 tcatgaatgc tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta
     3421 agctgacacc gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt
     3481 tatctgcaga agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta
     3541 ctgtttatcg aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta
     3601 atgatggatt agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt
     3661 tactatgctt gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg
     3721 agcaagtagt agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg
     3781 gaatctgtca ggaatgctat gaaaaagaaa
//
LOCUS       DACCDF010000140         3799 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCDF010000140 DACCDF010000000
VERSION     DACCDF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.148"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(22..636)
                     /locus_tag="IVK51_002684"
     CDS             complement(22..636)
                     /locus_tag="IVK51_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432224.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            950..1372
                     /locus_tag="IVK51_002685"
     CDS             950..1372
                     /locus_tag="IVK51_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432225.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1382..1585
                     /locus_tag="IVK51_002686"
     CDS             1382..1585
                     /locus_tag="IVK51_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432226.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1796..2380
                     /locus_tag="IVK51_002687"
     CDS             1796..2380
                     /locus_tag="IVK51_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432227.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            2549..>3799
                     /locus_tag="IVK51_002688"
     CDS             2549..>3799
                     /locus_tag="IVK51_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP6432228.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLE"
BASE COUNT         1282 a          602 c          716 g         1199 t
ORIGIN      
        1 taataaaagc agcatgatca actatttgtc attgaccatg ctgtcttttt ttattctata
       61 cacggtttgt ctcgtaacat tgtagtcttt agcaatctta gcaatcgcaa caccattttt
      121 aagatcttca acgatgcttt tgtaaacaag gcgacgttgg ggatcttttg cgtctgcact
      181 gtataatttg ggccgccctt tgtagacgcc gtttgctttt gcgatcttaa ttccttgtgc
      241 ctggcgacgt ttcgattcag tccgttcttg ttcagcgatc atggccaaaa tttggatgat
      301 cagatcctta atgaatttat ccaggagagg attgccaatt gcttcagcca tgatcggtaa
      361 actagtaatg atcagcttca catttttctt cttcaagtaa ttgactgaag tgatgatctc
      421 gtcataattt cttccaagtc gatcaatagc ttccacaata aaaatatctt gatctcggac
      481 gtaatctaat gcttcttgga aaacaggtcg atgagtaatc gttgcgccag attgtttctc
      541 gatgaaaatt ctctctgcac cgaatttttt catttcttca atctggcgtt gttcattttg
      601 atcgatcgat gagacacgta tgtatgcaac tctcaaaact tttacctact ttctgcaact
      661 atttaaaata caccctaatc atacacgagg aagccatatt tttcaatgtt ataccagtgt
      721 ctatttaggg taaaccttat atagacaaaa actaatgatg gaggaaatag tatgtgtcac
      781 ttttcttttt aaatggcaat attttttgta aataaatgtc aatcaagaaa atttaaacga
      841 ttatgacatt aaaaatatat tttatgaatt ttctagtata taacttgatt tatctgttaa
      901 gatttattaa gaaaacgttt tcttattttt gatttcgagg tgatagacaa tgaaaaataa
      961 gtttatttta tctttaggta ttttaactat tggaattagt gtaactggaa ctacaattcc
     1021 tacatttgct gatagtacaa atgaaccaac tactgtagta gataataacc aagaattaac
     1081 tcctttagat aggaacggaa tagccattgg taacccaact aacgacttaa ctgttactga
     1141 aaattattta atagtttctc cattacttgc tcgagcagct tctaaaacga tacgagtgac
     1201 aggaacgatg atttttgata aagttggttg gccttctaga cctcctcaat cacggaattg
     1261 gaaagaaaaa agaaatggtt attggtacta tggaaaattg tatttacaaa gttataaatc
     1321 agctggaaac aattggattg cgacttattc aggaacctta actagaaagt agggattttt
     1381 tatgccagaa tttgtgaaca caccaaattt ttatcaagat agcgatattc taagtaaaat
     1441 taatttaccg aaagaagaat cagctttaca agttcagcga actttacagg agacacaaaa
     1501 aattcaaaaa tcaatttcta atctagaaga ttctgaagaa aaaagaaaaa aaagagacca
     1561 aaaaacatat caaaaagaaa aataatgatt ttaaaaaaag agtgtctagt cgacattctt
     1621 tttttctaat tgaaagtgtt cataaaatca cccatttctg gacactttct ttttttgctt
     1681 tatttttcaa aaaaacaatt catttcatgt tcataaaccc ataacaatat ctatgttcag
     1741 ttaaactata tggataaagt tatgttacaa taaaattaat gaaggagtga tttttttgaa
     1801 aataggatac gcccgggtat ctactggttt acaaaatttg gatttacaag aagacagcct
     1861 aaataaattt ggttgtgaaa aaatttttac tgatcatatg agtggggcaa aaagtaatcg
     1921 accaggatta gaaatggcca ttgattttgt tcgctctggc gatacacttg tcgtttggcg
     1981 cttagatcgt ttaggtagaa atatggaaga tttgatttca atcgtcaatc gactgaatga
     2041 gcgtggtgtg agttttcata gtattcaaga aaatattacg atggacaaat caagttctac
     2101 aggacagctc atgtttcatt tgtttgcggc atttgcagaa tttgagcgta atttgatcct
     2161 ggaacgatcc gcagcaggac gagcagctgc tcgtgcaaga ggacggtttg gtggtcgtcc
     2221 tgaaaaattg tctgaacaag atttggaatt actgaagact ttagtggata gcggtacacc
     2281 tattaagact attgcggaac ggtggaacgt ctcaagaacc accatttatc gttatttggc
     2341 aaaaatggag gagaaaaatg gaaagacaaa ttctcattag tttttgtcat ttaatgctga
     2401 aaatttttgc catttatcta gaaaagtgac aatagtatga gaattttttg taagtgtcta
     2461 ctgataactt attctcaaat atttagctat ttcttcttta caacatcgaa catatgtttg
     2521 tataatgtgg aagaatggag tgttatttat gatggattat tcaaaagagc cagtaaatga
     2581 ttacttttta atcgatatga aaagctttta cgcaagtgtc gaatgtatag aaagaaattt
     2641 agatccatta acaacagaac ttgttgttat gagtcgatct gataatactg gttcaggatt
     2701 gatattagct tcttctcctg aagcaaaaaa gcggtatggt attacaaatg tgagtagacc
     2761 acgtgactta ccacaaccat ttcctaaaac actacacgtt gttccaccac gtatgaacct
     2821 atatatcaag cgaaatatgc aggtaaataa tattttcaga agatatgtgg ctgatgaaga
     2881 tctactgatt tactcgatcg atgaatcgat tttaaaagtg acaaaatcac tgaatctttt
     2941 tacgactgaa gaaacacgaa gtcagcgaag gaagaagctc gctcaaatga tccaagaacg
     3001 tattaaagaa gagttaggtt tgattgctac ggtaggcgtc ggcgataatc cccttttggc
     3061 taaattagcc ttagacaatg aagcaaagca taatgaagga tttattgcgg aatggacgta
     3121 cgaaaacgtt cctgagaaag tatggaatat tcccgaaatg acggactttt ggggaattgg
     3181 ttcgcgaatg aaaaaaaggc tcaaccaaat gggaattttg agcattagag accttgctaa
     3241 ctggaatcct tatacgatta agaatagatt aggtgtgatt ggattacagc tgtacttcca
     3301 tgctaatggg atcgatcgaa cagatatagc aatcccacca gaaccgacaa aggaaaaatc
     3361 atatggaaat tcacaagtgt taccacgaga ttatactaga agaaatgaaa tcgagctggt
     3421 ggtaaaagaa atggcggaac aagtagcgat acgcattagg cagcataact gtaaaacagg
     3481 ttgtgtccat ttacacattg gcacgtcgat cttagaaacg agaccagggt tttcacacca
     3541 gatgaagatc cctatcacag ataatacaaa ggaattgcag aattattgtc ttttcttgtt
     3601 tgataagtat tatgaaggac aagaagtaag gcatgttgga ataacctata gcaagcttgt
     3661 gtacaccgat tcgcttcagc tggatttatt tagtgatcca caaaagcaaa ttgacgaaga
     3721 aaatttagat aaaattattg acaagattcg gcaaaaatat ggttttacgt cgattgtaca
     3781 tgcttcttca atgttagaa
//
LOCUS       DACCDF010000141         3743 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCDF010000141 DACCDF010000000
VERSION     DACCDF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.149"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..144
                     /locus_tag="IVK51_002689"
     CDS             <1..144
                     /locus_tag="IVK51_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6432229.1"
                     /translation="YRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
     gene            complement(760..1800)
                     /locus_tag="IVK51_002690"
     CDS             complement(760..1800)
                     /locus_tag="IVK51_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP6432230.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2149..2475)
                     /locus_tag="IVK51_002691"
     CDS             complement(2149..2475)
                     /locus_tag="IVK51_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432231.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2462..3250)
                     /locus_tag="IVK51_002692"
     CDS             complement(2462..3250)
                     /locus_tag="IVK51_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6432232.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1148 a          623 c          526 g         1446 t
ORIGIN      
        1 tatcgaagtt tacgcactgc ctctaccacg attaaaggca tggaagccat tcgaggatta
       61 tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac tgaaatcaag
      121 gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat tctttattta
      181 aaactttgca acagaaccgt ccgttgcggg taataccgta acactgaagg gcacgcatta
      241 aggcgtcatc ttaatgcttt cctgtaggac actgacggag attaccagtc tcccgagcct
      301 tacaggggcc tttctttttt ttgtattttc aattatcaca atttatcgtc caaaagtgca
      361 gaacgaaaat caattcatca caagcatatc agacaattaa atttaaaaca actaaaaata
      421 tacaaaaaag tgtaattgaa tattagcagc actgcctgaa aagaagtaat atcaggcttt
      481 tcagcttggg gaaataagtg aattacctct cttcatgaat tattataact caaaaaagca
      541 catatgcaaa cactagttcc ctattttttt gaatgaaaaa taactaaaat atctaacgat
      601 tatgacttta aaacctacgt ttgtgattgt tttcataaac ttttaatcta aaaactccgg
      661 aataatagct atttatttgt aacatttgta ataaaattaa aatcataaaa aagttctaat
      721 ttaaaattga tcatttttat agtgatcgct ttttatcaac tactctagcc aattttccgt
      781 actaactgtt ggtaattcgt taaatgtttc tctttgttcg tttgcaagct ttgccgcaat
      841 agctttttcg acaaaccaat tttttacata aacgaaaatt agattctgca aattgtttac
      901 tttttcagtt ttttgttttt gataagcttt cagcaaagtc atatataatc ctttgtcagc
      961 atcaattcca taagattcca gttcttcata aacaacaact gtttgctcta attcttcagc
     1021 ttttttcttt gcattatgaa tagtttggac ggttttttga gcgtcttcgt aagtactaga
     1081 aaatgttttt atcaagttta atacattttc tggtacaaat gtatttatac tgtcctcttt
     1141 catgatgtcg acgaagttat ctagtaaaac ttgattttgt aactgatcag tttcagtgtc
     1201 tattatgtgt ctattagtgt ctatagaatt agaatagaat agattgtctg cgattttcgc
     1261 aattacggtt gagtccttga tatgactgga ttttgccgta tttgcgattt tcgcaattac
     1321 ggtggtatca ttgatttctc gcggttttgc cgtatttgcg attttcgcaa ttacggtggt
     1381 atcagtattg tcttcctctt ccatgatttt aaagacgtct tgattcgtta cttctggctt
     1441 gcctaagtac aataaattag gcatggattc aatcttaccg tcacgtttat ttactcttcc
     1501 tctcttctgt ttcagaagac ctgctttttc taattccttt ttgattttag atactttacc
     1561 ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg aatatgaaaa atatattatc
     1621 atcagaatct acccagttat ttttgactga ataatcaaat cgatctttaa gtagcatata
     1681 agcaattttt gcatcatttg atagatccat gtatttttcg ttagtaaaaa ataccttagg
     1741 catttgataa tatttttcac gataaatttc ttttgatttg taataattaa aatcattcat
     1801 agccaatcct ctttctgtag gatagcgttt tgttcaaaac tatgttacaa tgaattaaac
     1861 aaatatatct atccactata tatttttcta ctgctgataa gtcgccaaac tttgttaaag
     1921 cagtagtctt tttttatctt tttttagttt accttaaaaa ataaattaaa gcaaaccgct
     1981 ctatttcaag gtttaagctc atagtaacta tatcacgata ttacaataat attatatcac
     2041 gatatcgtaa tattacaata tgaagataag cgacttatca gctttgatac tactgatttt
     2101 tataaaaaaa atatcacgat attatgatat cgtgatattg aatgtaaatt aaattttatt
     2161 ttgtttcttt gctagtttat atccttcaat caagtattct tcaggtatgg actttaatag
     2221 cgcatcttct ataacgtctg gtttactttt gtctgattca gcaatagtcg tccaatattt
     2281 gagtgctgac attagaattg gatttatacg aatgctatct gctgttctta cactacttcc
     2341 ttgatctttt atgactttac ttatattttt cttggttaat tcgatttctt ctacttcttt
     2401 tgatttaaaa ttctttttat ttttatttcc tagtaatcta cttactgcag cttgactgcg
     2461 attatcttct gccatcaaaa tcaccaactt cttttaaata ttcttctgta aatgctttat
     2521 agtcatcttc agctgttgag ggtttattaa aattgattag atctgatcca gcagcttgcg
     2581 cttcgtcaac ggttattgtc ctacgaatat aagttttgaa aatttttgca ccaatgactc
     2641 tagaaatttc ggttgctgca gacgtcattt ctttagaaat gttataacgc ttattatact
     2701 tagtaaatag aactcctcgt acttcaagct tgtcgttcgt atattctttt accgtttcaa
     2761 tagtttcact taactttatt agtcctttta atgagaaata cgcagcttca actggaatga
     2821 taatttcatc agaaacagta aatgcgttga ctgtaagcat tcctaaactt ggtggacaat
     2881 caattataat ataatcatag ttgtccttga ttggttgaag tactctttta agcctgtgtt
     2941 cacttcctac ttgagtaaat tccctctctg cagaagaaag ggctatgtcg gctggcaaaa
     3001 catcaattcc tttatagtcc agaatagcat ctgtaatgtc agcctcccct tttaatactt
     3061 cataaattgt aggttggttt gttccatcta tatcgaaacc tacattatct gtagcatttc
     3121 cctgaggatc aatgtccact aaaagtacat ttttaccgta aacaaatttc aaacatgctg
     3181 ctaaattaac gctagtcgta gttttagaaa ctccaccttt ttgattggct actgacaata
     3241 ttcttgccat taaaatatcc cccattcatc ttatattata atataattat atcataatat
     3301 tgtgatattg caatatcgta tatgcattat attgtaatat tgtgtgatta ttttgtttta
     3361 aagatatata tcggttcgga tataaagatt attcatatgt tattcgaaat taaaataatc
     3421 actttttatt actttaaaat tttttattaa tgataagaaa tatgttttgc gagcagcaac
     3481 tagaataatt taaacattac taaactgtct atattgttac taataattcc cttcttatag
     3541 tgcattttct tagaaaaaaa caatattacg atatcatgat attaagatat cgtaatatta
     3601 tacaattgtg attgatatta tatatgtttt tttatttttt tatcatgttt aatcttgaag
     3661 gaatcatatt ctttttctaa agacaggagc ttttcataaa catcagacat gtttaaacta
     3721 gaggccaggc ttaaatcata aac
//
LOCUS       DACCDF010000142         3682 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCDF010000142 DACCDF010000000
VERSION     DACCDF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.150"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..2279)
                     /locus_tag="IVK51_002693"
     CDS             complement(<1..2279)
                     /locus_tag="IVK51_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432233.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAP"
     gene            complement(2951..3219)
                     /locus_tag="IVK51_002694"
                     /pseudo
     CDS             complement(2951..3219)
                     /locus_tag="IVK51_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1057 a          801 c          568 g         1256 t
ORIGIN      
        1 ggtgctaaac cgtcaattgc cagttttcca tcttctcctg tagttaaacc ggtttgtagc
       61 gtttctcctt ctcggttctg taactcaaaa acagctcctt gaaggacttc accactgcct
      121 tcagcatgtt taattagttc aacaccaccc gtatcaagga tgttttcaat ggttaacgtc
      181 acttcttcta tggagtattc atttataaaa acatcatgta ccgtcgcatc taaaagatac
      241 ccttccggtg cttctgtttc tcggacttga tagctaccta caggaagcgg ttcacttaaa
      301 aattctccgt tttctgccgt tataccttct tgtatcactt gtgaatcgct tgataaaatt
      361 tcaaattttg ctcctgccag cctttggcca ttctcatcag ttttaattac tttaatacgc
      421 ccatcgattt tccttgttct tacagtactc gtattcaatc gattgtcact attcacaagc
      481 gttgcaaagt ttaaaatttg atcgccgacc gcatacgcct ctacattgac cttaaaagag
      541 agggtaaaac ttgtcggacc accaatcgta ttattcgcac tgaaacgaac tattccatta
      601 ttgacttcgg catcaatgaa agagggaata tctgatagaa atgacacaaa ttccatgcct
      661 tcttccaaaa catcttcaat aacaagcgta tttcttgttc cagaaaacat cgagacatgc
      721 agagtatacg ttagttcatt ttcattgaga ttgataacat ttttgtccac actttttctt
      781 aaagagattg ccggaagatt actttcaggg attctcaagc ggtagtctcg gctcattacc
      841 tgattattgt aggtcatact cgctcgattc cgagaaaacc catcctcttg tcttccttca
      901 ttgaacaagt cgacatcggt cacttcaaga gcatattcaa taaaatacga ttgaccctct
      961 gctagattac caaagtccac aacaagtgta ttcgtttcct tattaaaaga tactttgtcc
     1021 acaaattcct cggaaacata atgagttcta acgtttgctt cacctggatt cgttacttct
     1081 gcgatccgaa ccccacctga aataggttct ctatttccag tggaactcca tgtagtcgga
     1141 ttcggtgcca aagttgttcc ttgcggtagt acatcggtaa ccactacacc gtcttttcgt
     1201 gatccttcat aatttacctg caaaataaat ttattactgt tggtcacgtc ttgcccgtca
     1261 atcagcagat tcccaagatt tccttggctg tcgatttctc tagagccata gtataatttc
     1321 gaaaaaatgg gtcttgatga aggaataggc gtaattgaaa attccgtgtt tgctgctcgc
     1381 tcagcataag taagactcac ttgaaagtca ttatttggct cagtaccgcc tgattgaaag
     1441 aaagcaactt caaacgtgat actgaatgta tacgactgag cggcttcatt taatccttca
     1501 taatcaaagg taaatggaaa aacaacgtct gtgccttctt ggtaaaatgc cagtggcttt
     1561 aaaggtgaat catcagcgac ctccactcta atgaactcaa ttaagccaat cgatgctcta
     1621 ggaactcgta cgaccatctc cccatattct tcaagaatat cgccagcgcc tccttgaatg
     1681 tatcccttta acgtttgtgt atgcctgctc ggcaaagtgg ggcgctcagc agagatgccg
     1741 atgtcataat tcaagagctc ctcttgtctt tcaatctgag attcctgcgt tatagatggc
     1801 tcagatgatc tggcttcatt aaaagattca cttaagcttt cttctggtat attaaaagat
     1861 tcacttgtgc tttcttctgg tacattaaaa gactcacttg tgctttcttc tggtacactg
     1921 atttctattg agaccgtatt tgattgaata tcaccgcttc ttgcggtcaa ttcgtattta
     1981 cctgaatgct gggaagaata agtaatcaga cgttcgtaat cattgagttg cttcacttcc
     2041 ccttcaatca aatcctcact cttatcaatg accaatgcat ttagcaggtc tggtgttcct
     2101 ctgactcgca actggaactg ctcacccaaa gcagcctcct cttgatcaac ttcgatcgtc
     2161 agcgaacgct ctggctgttc tgctaaagac tcagctagaa ccgttatgtt cccaaattga
     2221 ttgatcacaa taagcataac tagaaacata cttaacagtt tatgaaattt gttcctcact
     2281 attactcccc cttataattt tgactaaaca acaaggtacc cgataatttt ctaaaaagat
     2341 tccccaactc aaaatggctg cgttacctcc ctcgtatttt cggttcaact caagcgttgc
     2401 aatgtaagct gtaaacaaag ctaatgattg ccgattgtct tctgttgatt gattcgatag
     2461 ctttgtatga cttttagttg tccgtgtggc ttaaccataa ttatgatttc acgattcctc
     2521 ctgccagtgc cttgcacaag ctggagcgta accctaatag ttattttgtt aatgtactta
     2581 tatactttta tacttttcca ttagaaacaa tcactaaatc gttgatacca aggtcagact
     2641 ctagaatttc atcagtgagt ccctgtattg gttaaccttc atcaatccca aaaaaatata
     2701 tcaatgctta gcatcaaaat gaatatatag tttagaatac ttgggattca tcgataactg
     2761 atcgcttatt tggccatatt atttggcact tggttcaact aaacgcattt aggatgttcc
     2821 ttcattctcg tcatggatgt tctcaaaccg tcattactaa taaactgtcc accgaatcga
     2881 tggttcattt cttgcatttt ccacgtccaa ttactctttt tgctctttat ttgggcgtag
     2941 tttcactttc ttaacttgta tcgtcttcca tcaactcctt aatcatcttc ttagctttat
     3001 tggctcggtt tccttgtaaa aggtatctac aaatagtgac aatctgtatt aaatcttcat
     3061 cattttctgt aatgtcaata atttctatat tctgtaccat atattgcaaa gggtttcact
     3121 aagttcataa ccgaagcgaa ttaaacaact tttgtataga ataactacct tttctatttt
     3181 tccattggta attctatcta ttaattggtt taatcctttt gttgtagttg attccactac
     3241 caagatgatt ctctcgaatt ttaaagcttg aagatgtatt gctatttttg ctaactggcg
     3301 tatttgcaac tatttttccc ttctattctt ccttcattga ttcccctcta aaattactat
     3361 ccataaaaaa ttgcataaat gacaatagta tttatattag tcatactgct ttttaacaca
     3421 gcaaattcca gaaaaacatt cgttacatat ttgtgaattt ttccaaaaaa taacattttt
     3481 ataaaacgac gaaaaataaa tcactaccag tcaacttcta taatattacc aaataacaaa
     3541 ctaactatcc atcaataacg tgttttattt tttaaaattt ctataaaaaa acaatcattc
     3601 ttaaaaagaa tatataaaaa taacaataac ctttgtgatt tttcttgttt gaagaatgtt
     3661 agaagaaaaa tctctaaata ct
//
LOCUS       DACCDF010000143         3681 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCDF010000143 DACCDF010000000
VERSION     DACCDF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.151"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..277
                     /locus_tag="IVK51_002695"
     CDS             <1..277
                     /locus_tag="IVK51_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP6432234.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IVK51_002696"
     CDS             420..641
                     /locus_tag="IVK51_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432235.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IVK51_002697"
     CDS             657..1499
                     /locus_tag="IVK51_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432236.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IVK51_002698"
     CDS             1562..3364
                     /locus_tag="IVK51_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP6432237.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYCWKYKD"
     gene            complement(3423..>3681)
                     /locus_tag="IVK51_002699"
     CDS             complement(3423..>3681)
                     /locus_tag="IVK51_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6432238.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSLLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1312 a          523 c          749 g         1097 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactgctgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aacaaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaatggata tagtaatgcc c
//
LOCUS       DACCDF010000144         3668 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCDF010000144 DACCDF010000000
VERSION     DACCDF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.152"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            170..514
                     /locus_tag="IVK51_002700"
     CDS             170..514
                     /locus_tag="IVK51_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP6432239.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IVK51_002701"
                     /pseudo
     CDS             515..934
                     /locus_tag="IVK51_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1026..1376)
                     /locus_tag="IVK51_002702"
     CDS             complement(1026..1376)
                     /locus_tag="IVK51_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432240.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IVK51_002703"
     CDS             complement(1342..2673)
                     /locus_tag="IVK51_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP6432241.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVNN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IVK51_002704"
     CDS             2929..3495
                     /locus_tag="IVK51_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP6432242.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         1364 a          619 c          708 g          977 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gtaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 attgacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccgg
//
LOCUS       DACCDF010000145         3564 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCDF010000145 DACCDF010000000
VERSION     DACCDF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.153"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..243
                     /locus_tag="IVK51_002705"
     CDS             <1..243
                     /locus_tag="IVK51_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432243.1"
                     /translation="FNFTENKWEEAVTQDYSKKIELLENLSIGLQVDNTALKKSNEEL
                     TEKADSMVQLNAKLMLNDLTINKKIEAIEKQIGGAS"
     gene            243..380
                     /locus_tag="IVK51_002706"
     CDS             243..380
                     /locus_tag="IVK51_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP6432244.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRQFVPLCITDEEADKIISKEESA
                     S"
     gene            418..711
                     /locus_tag="IVK51_002707"
     CDS             418..711
                     /locus_tag="IVK51_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432245.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKIAETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            734..931
                     /locus_tag="IVK51_002708"
     CDS             734..931
                     /locus_tag="IVK51_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6432246.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            942..1961
                     /locus_tag="IVK51_002709"
     CDS             942..1961
                     /locus_tag="IVK51_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432247.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYQYEYIFIMDYNTGKELARVNANGVS
                     RPDVNQTYGTYGNVGYHVSFNMRNFPNKKVYVMMRATNDPKGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(2068..2616)
                     /locus_tag="IVK51_002710"
     CDS             complement(2068..2616)
                     /locus_tag="IVK51_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432248.1"
                     /translation="MPKDLKKTYNQYLTNLDGKTAILTTPFTPLDCFYIKFDILQLPH
                     LLGLHKIYNEPPKSLCSKLASSSITYEKIQRHKMFGSIKDRITLFDFILDIFLEDYNG
                     SVIYVSEADRNGSSMKLDIAFNHPYKNKILTLGLREISPAIYIPVTFYVKKKIIAQDF
                     QQSKRAKILSLEFIGTKNILLR"
     gene            3338..3411
                     /locus_tag="IVK51_002711"
     tRNA            3338..3411
                     /locus_tag="IVK51_002711"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3372..3374,aa:Ile,seq:cat)
BASE COUNT         1250 a          553 c          676 g         1085 t
ORIGIN      
        1 tttaacttca ctgaaaataa gtgggaagaa gcagttacac aagattattc taaaaaaata
       61 gaactactag aaaatttatc tataggttta caggtagata atactgcatt gaaaaaatca
      121 aatgaagagt taacggaaaa agcagattcg atggtgcagt tgaacgccaa actgatgctt
      181 aatgatttaa caattaataa aaaaattgaa gcaatagaaa aacaaattgg aggtgcttca
      241 taatgtttag ttttagcgat gtaaaaatga tgtacgattg gggctgtttt actgacgatc
      301 aagttcgaca attcgttcca ctatgcatta cagacgaaga agcagataaa atcattagca
      361 aagaagagag cgcatcttaa ttgatgtgct ttttattttg attcaaggag ttgtcacatg
      421 attaatttag gagaatgggg aacaatcgca ggatcaatca ctgcgattgt ttctttgatt
      481 ttattagtaa taagaccagt cactgcatct ttctcgaaga ttgctgaaac tctttcaaaa
      541 gtaagtcaca atttagattt gctgactaaa gatttagaat cgagcaaatc agatcgattg
      601 atgattcatg aagaactaaa gaaacatgat gaaagattag atacacatgc agaaaaattg
      661 gtagaacaca cacaacaaat taaaacttta tttagagaaa gatctcggta aaaaagaaag
      721 gagttaagaa gaaatgattt tacccgataa gtattatcaa gttattaaat ggacggtttt
      781 aacagtttta ccagctgcat ctgctttagt agccatgtta ggcaaagcat atggatggaa
      841 tggaacagat atgacagttc tgactatcaa tgcagtagcc acgtttttag gtgttatcac
      901 tggtgtgtcg gcttataatt tgaaaaaata ggaggaaaca aatgaaaaag aaaattacta
      961 ttactgcgat gagcctgtta acggctcttt ttttattgcc gattaatggg tttgcctata
     1021 cgattaacaa tgaatttaat ttgggcccaa acgaaggtag ctcacaagta gcaaataatc
     1081 agtacatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     1141 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     1201 tttatcaagt cggtcaacct ggttatgtac agtacggtgc tggttcgtat gctaacgcta
     1261 acagtcctgt gcagattgag ttacagcaca cacatgataa agcaactttt gaaaagaact
     1321 ataaagcata cgttgaattg gctagagatt cagcgatgaa atatggtatt ccattaacat
     1381 tggacacgcc ttataaccaa ccaggaatca aatcgcattt atgggtaaca caaaacatct
     1441 ggggcgatca tacagatcct tatggttatc tttctgaaat gggcgtaagt aaagaaaaat
     1501 tagcatatga tttagctcat ggatttaccg atgaaaatcc gacaacttca gatgataaac
     1561 cagtcattga tccaactcga gcaggtgctg caaatcctac gctgacagat ggaacaaatt
     1621 acgcccacat tgatcagttc ggagaaatcg aaaacgcaaa cttgcatgtt gctggatggc
     1681 atattgctaa ctatcaatac gagtatattt tcattatgga ttacaatact ggaaaagaac
     1741 tagcacgagt aaatgctaat ggcgtttcac gcccagacgt aaaccaaacc tatggcactt
     1801 atggaaacgt tggttatcat gtatcgttca acatgcgtaa ttttcctaat aagaaagtct
     1861 atgtaatgat gcgtgcaacg aatgatccaa aagggaacac taaaggcgga gcacaagatt
     1921 ttcatgataa acgctggtat ttaaatattc cgcaacgata aaaatagccc ctcgatgagg
     1981 ggtggtacat aacgatattg actattatca gttgtctttt tacgctttgt gacaacttgt
     2041 attggttata gtagataaat tctaaagcta tcgtaagaga atgtttttag ttcctataaa
     2101 ctctaaactt agaattttag ctcttttaga ttgctggaag tcttgggcta taattttttt
     2161 cttaacgtaa aaggttaccg ggatatatat agcgggggat atttctctta aaccaagggt
     2221 caatatttta tttttataag gatgattaaa tgcgatatct aatttcatag aagaaccgtt
     2281 tctatcagct tcagaaacat aaataacgga tccgttataa tcttctagga atatatctaa
     2341 aatgaaatca aacagagtaa ttctatcttt gatagagcca aacattttat gtcgctgaat
     2401 tttttcatat gtaattgaag aagaagccag tttactgcaa agagatttag gaggttcatt
     2461 atatattttg tgtagtccaa gcaaatgtgg aagttgaaga atatcaaatt ttatgtaaaa
     2521 gcaatctaga ggggtgaatg gggttgttaa aattgccgtt ttgccatcaa gatttgttag
     2581 atattgatta taagtttttt ttaaatcttt aggcatagaa ggcgctcctc tgtttagttt
     2641 ttaacaaaaa aaaagagagt gcaaagtatg gataccgtcc catacagacc aacgctaggc
     2701 taacccgcct gcgggggaac gtgagtgcac tcttctcact ttgattgata tgattattat
     2761 caatctagag ctaactgaaa gatgaggtga tagatctatc ctcgtaatta atataacatt
     2821 tacgtaaaat ttatgcaagc gtaaaatata aaaaacaata agatgaaaaa ttaagaaaac
     2881 ttaatatata cgatttaata taactatttg tacgtgtggc acattatttt gatgcaatat
     2941 aattacaaat tataagtgat aaaataacgt ttttttgaat attgtaattc ttgctaataa
     3001 aaaaccattg atctcggaaa tcaatggcaa ggttatagat tctagacact agaattcaag
     3061 actaagctag ggattgaaca gattggatat ccgcgcaagt gcttgtaggc catgttgaca
     3121 gcatggtctt tttttgctta caatgtcatt ctatactacg taaaaaaagc gtcaataatt
     3181 tttttactat aaagctaagc aaatgaatgt taaatatact ctattttttc ttttagagtt
     3241 atgcaataat aaatatgcca tcacaacaaa gaatgaaacc cattattatc tagtctatct
     3301 ccattctttt tgttagcagg agttgtggtg gctttccgta cccttagctc agttggttag
     3361 agcagacggc tcataaccgt ccgatcgtag gctcgagtcc tacagtgtac atactctaag
     3421 tgatttaaat tgcgaataaa atataaaaat gactcccctt ttgactcccc caagcagtat
     3481 atggatgtct ctaaaagttg tttttttaga taataaaaag ctgataataa gcgaaaaaca
     3541 cttaaaatca gctttaaacc ctat
//
LOCUS       DACCDF010000146         3476 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCDF010000146 DACCDF010000000
VERSION     DACCDF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.154"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            647..964
                     /locus_tag="IVK51_002712"
     CDS             647..964
                     /locus_tag="IVK51_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP6432249.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLIGLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IVK51_002713"
     CDS             complement(1055..1420)
                     /locus_tag="IVK51_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432250.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IVK51_002714"
     CDS             1628..2110
                     /locus_tag="IVK51_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432251.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IVK51_002715"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IVK51_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP6432252.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1190 a          536 c          641 g         1109 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt ggtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tgtgaaaaat agttcctcat
     3301 attttgccgt tttttgagct aataccgagg gtcagctgat tgaacaccgt ttattcgaaa
     3361 aaaagagcta cgacatagaa ctgtcgtaac tttttttggc tcttcgtcaa atcgtgttgg
     3421 tgaagacaaa tttgaataaa acttagtccg atatgcggcg cgttctgtgc gccgca
//
LOCUS       DACCDF010000147         3460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCDF010000147 DACCDF010000000
VERSION     DACCDF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.155"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(862..1203)
                     /locus_tag="IVK51_002716"
     CDS             complement(862..1203)
                     /locus_tag="IVK51_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP6432253.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1203..1433)
                     /locus_tag="IVK51_002717"
     CDS             complement(1203..1433)
                     /locus_tag="IVK51_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6432254.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIGDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1720..2058
                     /locus_tag="IVK51_002718"
     CDS             1720..2058
                     /locus_tag="IVK51_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432255.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2263..2502)
                     /locus_tag="IVK51_002719"
     CDS             complement(2263..2502)
                     /locus_tag="IVK51_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP6432256.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2478..2825)
                     /locus_tag="IVK51_002720"
     CDS             complement(2478..2825)
                     /locus_tag="IVK51_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP6432257.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT         1089 a          647 c          537 g         1187 t
ORIGIN      
        1 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
       61 aataatattg acaaaaagtg actcaaaata aaaaaaaact tacattatac tggaatgaac
      121 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
      181 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      241 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      301 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      361 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      421 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      481 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      541 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      601 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      661 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      721 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      781 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      841 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      901 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      961 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
     1021 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
     1081 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1141 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1201 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1261 tcggcctctt ccccaccact aaatggatct ccaatttttg gaactaacgt aattgttcca
     1321 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1381 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1441 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
     1501 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
     1561 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
     1621 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
     1681 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
     1741 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
     1801 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
     1861 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
     1921 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
     1981 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
     2041 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
     2101 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
     2161 tatatgcagc ttatgagtat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
     2221 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
     2281 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
     2341 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
     2401 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
     2461 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
     2521 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
     2581 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
     2641 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
     2701 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
     2761 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
     2821 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
     2881 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
     2941 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
     3001 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
     3061 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
     3121 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     3181 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     3241 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
     3301 acctctccac taaacgatgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
     3361 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
     3421 caacctctaa tacacggtgt tccagaagta gcttcttcga
//
LOCUS       DACCDF010000148         3449 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCDF010000148 DACCDF010000000
VERSION     DACCDF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.156"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            116..976
                     /locus_tag="IVK51_002721"
                     /pseudo
     CDS             116..976
                     /locus_tag="IVK51_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            988..1815
                     /gene="panB"
                     /locus_tag="IVK51_002722"
     CDS             988..1815
                     /gene="panB"
                     /locus_tag="IVK51_002722"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAP6432258.1"
                     /translation="MKNTAVTFKESKLRNEKLTMLTAYDYSTAKIIDEAGINGILVGD
                     SLGMVCLGHEDTLSVTMEDMIHHTRAVTRGAKNTLVVADMPFMSYQTSVYDSVVNAGR
                     LIKEGRAQVVKLEGGIEVCDKIEAIVKASIPVMAHIGLTPQSVNAFGGFKVQGKDKEA
                     AKELIRAAKAVEKAGAFAVVLECVPTKLAELISKEISIPTIGIGAGAGCDGQILVYQD
                     MLGMYSDFTPKFVKKYANLSEEMNKAFTKYIEEVKDGVFPGPEHGFKISDDVLEKLY"
     gene            1830..2678
                     /locus_tag="IVK51_002723"
     CDS             1830..2678
                     /locus_tag="IVK51_002723"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAP6432259.1"
                     /translation="MVIFKTVNDVRSQVKEWKKQGLKVGLVPTMGYLHEGHESLIRKA
                     SKENDKVVVSIFVNPTQFGKNEDLGSYPRDLERDIEVCTRGRATAIFNPEVEEMYCDN
                     ASTFVNITGLTEGLCGASRPIHFRGVCTVVSKLFNIIPADRAYFGEKDAQQLAVIKRM
                     VRDLNIDIDVVGCPIIREEDGLAKSSRNTYLSLEERSSATILNKSLTLAKEALNNGER
                     DSLKIIEIISKNINTYNLAKIDYVEVVDSLSLQRVNYIEKSVLVAIAVFIGKTRLIDN
                     FTFELQ"
     gene            2688..3083
                     /locus_tag="IVK51_002724"
     CDS             2688..3083
                     /locus_tag="IVK51_002724"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAP6432260.1"
                     /translation="MKVTMLKGKIHRATVEQAELDYVGSITVDMNLLEAAGIYEYEKV
                     QIVDVNNGNRFETYTIAGERGTGMICLNGAAARCVSTGDKIILMAYCELNENEVKDHR
                     PKVVFVDDNNKVERVTSYEKHGRLSDIIL"
     gene            3285..>3449
                     /locus_tag="IVK51_002725"
     CDS             3285..>3449
                     /locus_tag="IVK51_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432261.1"
                     /translation="MSRRQRRTYSKEFKQQIVNLYLAGKPRAEIIREYELTPSSFDKW
                     MKQAQSTGSFK"
BASE COUNT         1336 a          374 c          687 g         1052 t
ORIGIN      
        1 gatattatga taaattaact atggatgata tttgaaaaaa acatactgta tatttgattt
       61 aatttttatt tttaagctca tatcgtaaga atgggacttg tagtctcact tctttatgga
      121 ggtgataatt atgaacgttg gttttattgg agccggcaag gtaggttgtt cattcgggaa
      181 gtatttccag gaacataaaa tacaagtaac aggattttat agtaaaagcg aagactcgtc
      241 attagcagct tcgaatttta catctagtaa acaatattta aacttaagag aattagtaga
      301 tgaaaatgat actattttca ttacgactcc tgatggtcaa attcaggagg tatggcaaga
      361 aattaaaaat tattaaatca aaaacaaatt aatctgccat tgcagtggtg caatatcttc
      421 agatatcttt tcaaatatac aggactatgg tgcttatggc tattctgtcc atcctatgtt
      481 ttcaatatca gataaatata attcatacaa aaaattaaaa aaatctttta taacaattga
      541 aggcgatgaa aaatatgcaa agtttttatg tgatttcttc aaaaagttag gtaatagtac
      601 tgtaattgta tctaaggaga ataaatctct ttatcatgcc gcatcagtag tatctagtaa
      661 ccttgtgcta ggacttatta atacaagcgt tacatatcta attcaatgtg gatttactga
      721 aaaaatggca attgaagctt tatatccatt aattgaattt aatattagaa atataaaaga
      781 aaaaggaatt atagaaagtt tgacagggcc agttgaacgt tgtgatattt caacaattaa
      841 aggtcactgt gaagttttat cagatgaaga cagagcttta tatatgctat tatcaaaaaa
      901 tgtattagag atagctatgt taaaaaatat taatagagat tatagtgaat tagaaaagta
      961 tttaggagag agttaggaga gaaatagatg aaaaatacag cagttacatt taaagaatct
     1021 aaattaagaa atgaaaaatt aacaatgctt acagcttatg actattcaac agcaaaaata
     1081 attgatgaag ctgggataaa tggaatatta gtaggagatt ctcttggtat ggtatgtcta
     1141 ggacatgaag atacactttc agtaacaatg gaagacatga ttcatcatac tagagcagtt
     1201 acaagaggag ctaagaacac tttagttgtt gcagatatgc catttatgtc ttatcaaact
     1261 tcagtatatg attcagtagt taatgcagga agattaataa aagaaggacg agctcaggtt
     1321 gttaaattag aaggagggat agaagtatgc gataaaatag aggctatagt aaaggcttct
     1381 atacctgtta tggcgcatat aggtttaaca cctcaatctg ttaatgcatt tggaggattt
     1441 aaggtacaag gaaaggacaa ggaagctgct aaagaattaa taagagctgc taaagctgta
     1501 gaaaaagcag gagcattcgc tgtagtgtta gaatgtgttc caactaagct tgcagagtta
     1561 atttctaaag agatatcaat acctacaatt ggtattggag caggtgcagg gtgtgatgga
     1621 caaattcttg tatatcaaga tatgttgggg atgtatagtg attttacccc taaatttgtg
     1681 aagaaatatg ctaatttatc agaagagatg aataaagcat ttacaaaata tatagaggaa
     1741 gtaaaagatg gagtttttcc aggtccagaa catgggttta aaataagtga tgatgtttta
     1801 gaaaaattat attaaaaggg tcaagaaata tggttatatt taaaacagtg aatgatgtta
     1861 gaagtcaagt aaaagagtgg aaaaaacaag gcttaaaggt aggattagtt cctactatgg
     1921 gatatttaca tgaaggtcat gaaagtttaa ttagaaaagc atcaaaagaa aatgataaag
     1981 ttgttgtaag tatctttgta aatccaactc agtttgggaa aaatgaagat ttaggtagtt
     2041 atccaagaga cttagaaaga gatatagaag tatgcacaag aggtagagca acagcaatat
     2101 ttaatcctga agttgaagag atgtattgtg acaatgcaag tacttttgtt aatataactg
     2161 gattaacaga aggtttatgt ggagctagta ggccaataca ttttagaggg gtttgcactg
     2221 ttgtaagtaa attatttaac ataataccag cagatagagc ttattttgga gaaaaggatg
     2281 cacaacagtt agctgtaatt aaaagaatgg ttagggacct taatattgat atagacgttg
     2341 tagggtgtcc aattataagg gaagaggatg gactagctaa aagttctaga aacacatact
     2401 taagtttaga ggaaagatct tcagctacta ttttaaataa atctttaact ttagctaagg
     2461 aagctttaaa taatggagag agagacagct taaaaataat tgagataata agtaaaaata
     2521 taaatactta taatttggca aagattgatt atgtagaagt tgttgattct ttatcattac
     2581 aaagagtaaa ttatattgaa aaatcagttt tagttgcaat agcagtattt ataggaaaaa
     2641 caagattaat agataatttt acatttgaat tacaataggg ggctataatg aaagttacaa
     2701 tgttaaaagg aaaaatacat agagctacag ttgaacaagc tgagttagat tatgtaggaa
     2761 gtattactgt tgatatgaat ttattagaag cagcaggaat atatgaatat gaaaaagtac
     2821 aaatagttga tgttaataat ggaaatagat ttgaaactta tacaatagct ggagagagag
     2881 gaacagggat gatttgcttg aatggagctg cagctagatg tgtatcaact ggagataaaa
     2941 taattttaat ggcatattgt gaattaaatg aaaatgaggt taaggatcat agacctaagg
     3001 ttgtttttgt cgatgataat aataaagttg aaagagtaac atcatatgaa aaacatggaa
     3061 ggttatcaga tataattctt taggttttgt aatgttattt atatatagct agactattat
     3121 tgaaatattt attgattact aattggatag atgtcaataa agtagacaca aaaagagaag
     3181 atacagactg tagacaaagt ccattttatg tgaatcggct acactagact agacagaaaa
     3241 aataaggtgt gtagaataat aaaaaacaca ctggaggatt tttcatgtca agacgtcaac
     3301 gaagaaccta ttcaaaagaa ttcaaacaac aaatcgtcaa tctctatctc gctggtaagc
     3361 ctcgcgcaga aattattcga gagtatgagc ttacgccttc ttctttcgat aaatggatga
     3421 agcaagcaca atcaacgggc tcattcaaa
//
LOCUS       DACCDF010000149         3392 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCDF010000149 DACCDF010000000
VERSION     DACCDF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.157"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(67..2559)
                     /locus_tag="IVK51_002726"
     CDS             complement(67..2559)
                     /locus_tag="IVK51_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP6432262.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2566..3033)
                     /locus_tag="IVK51_002727"
     CDS             complement(2566..3033)
                     /locus_tag="IVK51_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP6432263.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3169..3242)
                     /locus_tag="IVK51_002728"
     tRNA            complement(3169..3242)
                     /locus_tag="IVK51_002728"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3206..3208),aa:Arg,seq:acg)
     gene            complement(3300..3373)
                     /locus_tag="IVK51_002729"
     tRNA            complement(3300..3373)
                     /locus_tag="IVK51_002729"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3337..3339),aa:Pro,seq:tgg)
BASE COUNT          927 a          750 c          557 g         1158 t
ORIGIN      
        1 aaatcttgtc acaacctctt aatcaagtct tactattata aaaattcagc tacacaggcc
       61 aagtaactat agggaaccta ctgtttcctt tttaggtccg cgaacattca atgtgatctt
      121 accttttgaa gcacctactg ttacgtggtc acctaaacga atttctccgc caagcaaagc
      181 ctcgctcaat cgatcttcaa tttctttttg aagtgcgcga cggattggac gtgcaccata
      241 ttcaggatca aatccagctt tcccgatcac atcgattgca gatggtgtga ttttcacttg
      301 aatatcttgt tctttcaatc gtttgataat cgctttgctc atgatttgga cgattgtatg
      361 gatttcatct ttactcaatg aatggaagac gacagtctca tcgattcgat tcaagaattc
      421 aggtcggaaa gctttcttta actcttctaa gatacgtttt tgcatggctt tgtgatcttt
      481 agtaacatct tggacattga atccgacatt tttttcttct cgaatttgtg tcgctccgat
      541 atttgaagtc ataatcatga ttgtatttct aaagtccact tttctacctt tagaatctgt
      601 taaatgacca tcatccaata cttgcagaag taaattgaaa acgtctggat gtgctttctc
      661 cacttcatct aagagaatca ctgaatatgg tttttgacga attttttctg tcaactgtcc
      721 accttcatca taccctacgt atcctggagg agacccgatt aatcggcttg tactgtattt
      781 ttccataaat tctgacatat caacacggat cagggcatct tcacttccaa acatgacttc
      841 tgaaagtgct ttggccaatt ctgtttttcc gacaccagtt ggtccaagga acatgaatga
      901 accaattgga cgatcaggat ctttcaatcc gcttcgtgca cggcggatcg cacgtgaaac
      961 tgctttgaca gcctcatctt gaccaacgac acgttcatgc agaagtcctt ccagttctaa
     1021 caagcgttcg ctttcttttt tctccaactg ctgtagaggg acaccagtcc actccgatac
     1081 aactgtagca acatcttctt ctgttacacg atcagaataa ccttgctcct gttttgcttc
     1141 aattgcagaa actttttgca gctttttacg tagtgctttt tcttgtctgc gtaattgagc
     1201 agcattttca aagtcttgtt tttggatcgc tgcttccttt tcttgtacta gttcatcgat
     1261 ttctagtttg atcacagttg attttgttaa gtgatcggtt tgatccaaac ggactttcgc
     1321 tgctgattca tcgatcaaat caattgcttt atcgggtagt tgacgagaag tgatataacg
     1381 aacagaaaga ttgactgctg ctcgtacagc ttcatctgtg atctccactc catggtgctc
     1441 ttcatatctt gtacgtaatc cttgaagaat cacttctgct tcttcaggag ttggctcatc
     1501 tacttgaatg cgagcaaaac gtcgctctaa tgcagaatct ttttcgatat atttttgata
     1561 ttcatctagg gtcgttgctc cgatcgtttg taattctcct cgagctaacg ccggtttcaa
     1621 gatattcgaa gcatcaattg ctccttctgc tccacctgca ccaattaagg tatgcaattc
     1681 atcaatgaat aagattactt gaccatcatt atagatttcg tcgatgacct ttttcattcg
     1741 atcttcaaat tcaccgcgat atttcgtacc agcaaccaaa gatcccatat caagcatcat
     1801 caggcgcttt tcttgcatgt cttctggcac ttcacctaaa atgatttttt gagctaaccc
     1861 ttcagcaatg gctgttttgc ctacaccagg ttcacccacc agtacagggt tattctttgt
     1921 ccgtcggcta agaatctgga taagccgttt cacttctctc gaacgaccaa caactgggtc
     1981 cagcttgttt tcttttgcta atttcgtcaa atcacgtgct aatgaatcta gcgtaggtgt
     2041 tccttgcggt gtttgccgtt tatcttgtac atttacattt ctacgacgat tcattccttt
     2101 tccagcagaa tcaccagcta gccccatttt ttgtttcaga acttgacggg cttttgaaag
     2161 gctgatacct aagttgatca tgattcgagt agccatgata ctttcatctt ttaataagct
     2221 catcaaaata tgttctgttc caactttttg agaaccagtt ttcttcactt cgctgctagc
     2281 taaagcaaaa acttgtttca ttcttggtga atatggaaga aagactcctt tagggtaaga
     2341 acggatacca ccataaccaa tcagatgttc aatttcttca taaatttccg tttcattcaa
     2401 atccatttca cgtaatactt ttccagcaat tccattttgc tcgaccacta aggctagtaa
     2461 aacatgttct gaactaactg attgatgttt caaccgtttt gcttcttctt gtgcaatttc
     2521 taatactgct tgggctcttt cagtaaaaag ttcttccatc taatctcact tcccatcttc
     2581 ataacttaaa cgttccagaa atgctcggag aatccgagca cgtgtatgat cttcatattc
     2641 attgaaattc aatgtgtttt ttgcaattgc actcaacatc aagtttcctt ctttttttgt
     2701 aattatttgg tcttcataaa gtttttgaat aatagcaaaa gcatttttct cactgattgt
     2761 atcatcaaat aactgattta tctgctcaag taattgtttt ttatctgaaa tccttacttt
     2821 tgcaattcgt atataaccac caccaccacg tttgctttct actagataac cacgttggat
     2881 ggtaaaacgg gtattaatca catagttgat ttgtgaagga acacagttaa agaggtctgc
     2941 catttcaatc cttcgaatct cgatcatttc actattttcc agaattttct ttatatatga
     3001 ttcaattaaa tccgaggtat tctgatgtgc catcttattc attccctttg actaatattg
     3061 accttaatta taacgaattt gataatagaa agaaagaaga acgctttttg ttaaataaat
     3121 cctttagaat caacaaaaaa aaatacggaa tatactattc cgtatcgacg cgcccaagag
     3181 gagtcgaacc cctaaccttt tgatccgtag tcaaacactc tatccagttg agctatgggc
     3241 gcatattcaa tttaaaaaaa ttataaatca tttgaaaaca aaaagcaaga taaaaaaaat
     3301 cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa accaagtgct ctaccaagct
     3361 gagctacttc ccgtttaaaa aataaaaaag ac
//
LOCUS       DACCDF010000150         3374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCDF010000150 DACCDF010000000
VERSION     DACCDF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.158"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK51_002730"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IVK51_002730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP6432264.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IVK51_002731"
     CDS             complement(2261..2512)
                     /locus_tag="IVK51_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP6432265.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IVK51_002732"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IVK51_002732"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP6432266.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          920 a          740 c          626 g         1088 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttt
//
LOCUS       DACCDF010000151         3295 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCDF010000151 DACCDF010000000
VERSION     DACCDF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.159"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            135..1103
                     /locus_tag="IVK51_002733"
     CDS             135..1103
                     /locus_tag="IVK51_002733"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP6432267.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(1420..1944)
                     /locus_tag="IVK51_002734"
     CDS             complement(1420..1944)
                     /locus_tag="IVK51_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP6432268.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            2231..>3295
                     /gene="scm"
                     /locus_tag="IVK51_002735"
     CDS             2231..>3295
                     /gene="scm"
                     /locus_tag="IVK51_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP6432269.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         1049 a          667 c          611 g          968 t
ORIGIN      
        1 aatgaacgcc caccctcttc ttttttctct ttccttcttt cgtttttgct ttatgtatca
       61 accgttgttt cttcgaaaac tattttgttc tctccttctt cctgatagaa tggatgggaa
      121 aggagcttga tgccatgaca acgaaagaca aggtacttca attattgaaa aagacaccag
      181 attttctttc cggagaaaag ttggcacagc aactggaagt ttcccggaca agtatttgga
      241 aagccatcaa agaattggaa aaggcaggct atcgttttga gcacgggcct acagggtatc
      301 gttacctgcc ctctgacgta ctagatgcaa aagaaatcca gcaagagcta cggtcgttta
      361 ttcctgattt gaaagtacaa acgatgttga cttctgaatc tacgatgaaa gatgccaaac
      421 tggcagcagc tcaaggagat tctgtttcga agttatttgt tgctgataca caagaacaag
      481 ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg acgaggaatc tacatgagtc
      541 tcttgctcca tccagacaaa cggtttgatg aattgcctca atatacgatc atcgcagcca
      601 gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc tgcttcgatc aaatgggtca
      661 atgatatcta catggatgga aaaaaagtct gtgggatttt atctgaagca gtcagtgata
      721 tggaaagcgg caggatcagt catatcatca ttgggatcgg gcttaatttt tctgttcctc
      781 aaaatcattt tccagctgat ctccaagaaa aagccatctc tgtctttccc gacggacaag
      841 ctagtgtcac aaggaatcag ctaatcagca aaatctggca agaattgttc acttatcttt
      901 ctcacccaga aaaagcgttg gacttctata aagaaaaatc attcgtattg ggaaaaaccg
      961 tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc aaaagaaatc ggtgatcatg
     1021 gcgaactgat cgtccaaaca aatgaacaga agatgagttt gtcttctggc gaaatcagtc
     1081 tcacatctat ccgtacgcaa taattatcag atttcacaaa aggacacctg atctcataca
     1141 gtgtcctttt ttctgaattc tcttcaaaag actctcctta ttctatcagc tgacagtttc
     1201 tctatcctct agaaaaaggt attctaaaaa tatctataaa gcatggtaca ctttctttaa
     1261 atgaatcaat gcaaaaaaca agagagtcat cagtttacac gatcaaaaga gatagcaggc
     1321 cgcttccttt ttcaatctta tagatagacc atgggtaacg ataaaaacaa aaaagaactt
     1381 gcgacttatt ttgccgtaag ttctttttta gctagtgcct taatctctta atcccaacaa
     1441 acgtctcgca tagatcacct gaccagagtg catagcagca tcatcaataa tagaaacgag
     1501 acgcattcct cgtgtgactg gtggtgtcca gttctcgtca attacttcat ctagctcgga
     1561 aggttccagc cttcttaagt aagaaatagc aaaatcagca gctcctcgca agtatcctag
     1621 cagatcttct atttgattag cttggatctg ctttgcttgt tccagactat gcttccaatc
     1681 ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc cagtcttgac tatgccaaat
     1741 tggagaagtt cctgctaaat cagcaatctg gaaatctaat tctcgagcgg tatgccaagc
     1801 cagccaagtc attgatttga tctgcggggc tctatcttcc actggaaaac gattggcctc
     1861 ctcaatagag acgccttcaa acaaacgttc aaaccgatcg tatgcccgat ccacactctc
     1921 gcatagcata tctaaataat ccatttattt tcctcctttt tatttatcct acttactttt
     1981 tcctaattct aggataccta cttatatgtt actacacacc cttatttttt acactttaac
     2041 agtcaccatc atcaatagtt tggagattga aagaaaaaga tagtgtcgaa ctatcaattg
     2101 aacaagaagc aacaatatga acgtttcatt aatattcttt aaataaacaa tgatttttca
     2161 aacgaacagt ttaagactag gagaaaaaga gtataatagg aaaatgtttg aaaggaaagg
     2221 aaggggaaaa atgaaaaaaa gggcaagttt actagtccta gttgctttgt tgttcaatat
     2281 cttttcgtat ggtgtggccg tttcagcgga aacatacatt cctaaagacc ctgggaatat
     2341 ttataatgat ctaactggta actatggctt gttaggaatt gctagtcaat tccacgtatt
     2401 tgctaaaaat aagacaactg tgaatgcaca tacagacgga aatatagcaa cacatgaact
     2461 tgatgcaaaa aataattttg gtacagagct tcatgaaggt ttattgaacc aagaaataaa
     2521 ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc atacctacag gtagcacaag
     2581 aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca cttcaagatg gtcatcctac
     2641 agtcaatgga tccaggcttg atcacttaac tccagatgat ttttataaag atcgtgctgg
     2701 gcatgaatat attgatttcg agcaggaatt tgctaaattg aatgtagcag caaatgatct
     2761 tgcaacgatc actccagcta aaacatatac tgctgcagat tttccagaca tgaacaatcg
     2821 gacaatcgat ttaactgatt tatcagatac cgggttttta ttggtaaata tcgatgcaga
     2881 agtgttaacg atgaatacgc cacttcaaat cattaatcca aatgatcaag tagttgtatt
     2941 taatgtcatc aacagttctt ctgcattgaa cgtacagtca ccaatcaaat acaatgatcg
     3001 ttcaaatcat gaaacagaag atttttcgga tgcgaatatc tcttggaatt ttggaaatga
     3061 gatgactgat ttgacaatca gtgctccttt tcaaggaaca atccttgcgc ctaatgctac
     3121 tatccgcatt actcaaaacc aagacggaac gattatagga acaaatgtga ttttagatgc
     3181 agctaccaat cgttgggatc ctaacgaaat atttatcagc gacaatggta cagatacgac
     3241 agacacaagc gacagtacag atacatctac cacaagtgat agctcagatt catca
//
LOCUS       DACCDF010000152         3293 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCDF010000152 DACCDF010000000
VERSION     DACCDF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.160"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            38..589
                     /locus_tag="IVK51_002736"
     CDS             38..589
                     /locus_tag="IVK51_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAP6432270.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1265..1531
                     /locus_tag="IVK51_002737"
     CDS             1265..1531
                     /locus_tag="IVK51_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432271.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1589..2101
                     /locus_tag="IVK51_002738"
     CDS             1589..2101
                     /locus_tag="IVK51_002738"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432272.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2098..3135
                     /locus_tag="IVK51_002739"
     CDS             2098..3135
                     /locus_tag="IVK51_002739"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6432273.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1213 a          555 c          654 g          871 t
ORIGIN      
        1 gagcgagcaa gacaggaacg agagaacaaa catactcatg aaaagactag aggcttcgac
       61 ctcgaacttt aataataagt tagatgtttt tgaagaaaag actaagcatg caagattaaa
      121 ctttgaaaca actgcaaagc actatattaa acgacttgat gaagataatt tgaaattaga
      181 ttttcaacaa gctattcaag atgaattaag cgatactaaa gacgaaatac gtgaagtgac
      241 gaaacaagcg agagaagaaa caaaagagta taaagaaatc ttagaaagta aaataaaaga
      301 ccataacaaa gtggttgata aatcgaatac agcgctaaaa gtgatgacaa aaggcgttac
      361 aaatatattt tttgtgctaa ttatttttgt acttgtaatg cttgtgacag gtccgatagg
      421 tcatttcttt ggtattgaac atttatatag ctttattaat ggttttattg atgaccacga
      481 aagtgcatgg cgatatttga tgttgatact ttatgcagta ccctatgtat tcttcgctat
      541 cattctgtgg ctcatagcat tgttttttag agtatttaat gacctttagg tagatttata
      601 tataaaaagg gctaaatacg caaaataccc ccttaaaatg caaaataaat gcaaataagg
      661 gggtaactcc ttatgaaaaa acaactaata gtattataac acgtttttat gccgagaaaa
      721 cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt tggacttgaa
      781 ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt aggttccgac
      841 caataaggaa attggaataa agcaacgaaa ggagttgagt aaaatgagtg agaaaacgaa
      901 gaaaatcatt cgagtatcat tagctttttc acaagtagta gtgcaatcat tgatagttta
      961 ttatacttac cagcaatata aattatctaa agagaattaa gttttgatta tgtaataagt
     1021 tttggtacta aaaaagactt gatctaatta gaccaagtct tttgttagtg ttatattaat
     1081 aacaaaataa aaataagaag tcgctcactc cccgaccaaa gtttgtgagc aaaataaaca
     1141 gtcgatgttt gtttcttaat tgtatatctt gatattaaac gatttaagat tattcttcaa
     1201 gatatatatt cgcaaatgta attggaaaag cgacttctta aacaaaatta aggagtcgct
     1261 ttttatggca aaaataacga aaaatgagca agaaaatttg gtggaaaatt acacaaaaaa
     1321 accggcgtac tctaatagcc ggttagacgc acatttcgtg tgcacttcta atccgaaatt
     1381 gagctttgac gctatgacga tagtcggaaa ccttaataaa aacagcgcta aaaagctatc
     1441 cgattttatg agtttagatc cacaaattag actttgggat atacttcaaa caaaatttaa
     1501 agctaaagca ctacaagacc ccctatgtta ggctagttgt cgtataggat aataagttat
     1561 aataaagact atacggagga tgattaccat gacaaataaa aacaatgaac gttactctac
     1621 cgaactgaaa gcgacagcca tcaagaaaat gatgccaccc gaaaataggg ggattcgcca
     1681 attaagtcaa actttgggca ttccagaagc gactctttgt tcttggcgta aaaaagctag
     1741 aattcaaggg aatactacac ccggaacgaa aaaaaatgtc gaaaaatggt caagtgaaga
     1801 taaattttta attgtgatgg aatcattctc aatgaaccaa gtagaacttg cggaatattg
     1861 ccgaaagaag ggcttattta aagaacaaat tgaggcttgg aagaaaacgt gcttatctgc
     1921 gaatgaacag gaagaaaatc gaacgagaga acttgcaact gaactaaaag aagagaaaaa
     1981 acaagcgcgt caattagaga aagaattacg tcataaggaa aaagcattag cggaagccgc
     2041 tgctctctta ttactaagaa aaaaagcaca cgcgatttgg ggggaccaag aggaagaatg
     2101 atttcatctt cagatcgcaa acaagctgtt gaacttattc aagaagccaa tcgcaatggg
     2161 gcccgtctca cctatgcctg taatgagctg aatattagcg ttcgaaccta cgaacgatgg
     2221 acgcgtgaag gtacgatagc gcatgatcaa cgccctttgg cgaaacgtcc tgtacccaaa
     2281 aataaactga cggatcaaga acgcgaaaaa ataatagaaa ccgtttcaaa aaaagaattt
     2341 atgaatttgc cacctagcca aattgtacca aaacttgcgg attgctcgat ttatattgca
     2401 tcagagtcta gcttctatcg tgtattgcga gaaaaaaata tgcaacatca ccgtggccga
     2461 agtcaagttt cacaaaaacg cataccgcca agtcaccttg cgacgaagcc aaatgaagtt
     2521 tggacttggg atattacttg gctaaaaggt ccaataaaag gactctttta ccggttatat
     2581 ctcatcattg atttatttag ccgtaaaatg gtaggctggg agatttggga atcagaagaa
     2641 gctcgtcatg ccgaaaaact tattaaacag acggtgctta aggaacgtat ccaaggacag
     2701 ccgttggttt tacactccga taatggcagc cctatgaaag ccgcgacttt tcaagtctta
     2761 ctcgaaaaat taggaattca aagttcttat tcaagaccac gagtgagtaa tgacaatcct
     2821 tattcggaag cggctttccg aacgttaaaa tatcgaccag aatatcccaa taacggcttt
     2881 aaaacaatag atgatgctcg aaaatggacg aaagagtttg tcgattggta taacgattct
     2941 catttgcata gtgggattca atttgtgtct cctgcagcgt gtcatgaagg acgacatctc
     3001 gctattttag agaaacgaaa gcaggtttac gaggacgcta aaaagcgcca tcctgaacgc
     3061 tggtcaaaag gtgttcgaaa ttggactcca catcaatcgg tggcgttgaa tcctttaaaa
     3121 gaaacagaaa gttaacagga gcataatctt tcaagattat caaataaccc tcgtcaccca
     3181 ataggggaaa aagttattct tcccctattg gataacgagg ccaaccgacc atagggaggt
     3241 tctgcttttt gataggtgga aaataaaaat acgacaacta tattgacaaa cac
//
LOCUS       DACCDF010000153         3270 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCDF010000153 DACCDF010000000
VERSION     DACCDF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.161"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..238
                     /locus_tag="IVK51_002740"
     CDS             <1..238
                     /locus_tag="IVK51_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432274.1"
                     /translation="SNQPRTVEVRETRAEASQGDKTQKINIIEERKERPNEKVIVEQE
                     VKEKKRPRFTYENNPLFQDISDKPNPLFDSLFKK"
     gene            252..440
                     /locus_tag="IVK51_002741"
     CDS             252..440
                     /locus_tag="IVK51_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432275.1"
                     /translation="MQLILQISTQDFLELAELCDSVKKATNEKITVTNYIELMIQEEL
                     MMKNEIESNFKCCSLEKM"
     gene            452..1063
                     /locus_tag="IVK51_002742"
     CDS             452..1063
                     /locus_tag="IVK51_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP6432276.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVALIKQLNQIGNNLNQAVKALNIIAKYFREQSENKKEK
                     SLHFSFDVSRYNIFKDELANSIAKKMMISYPDDIFLESNRSTSSFLKLSEVEKDYFQY
                     WLEKQTRPDTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            1048..2439
                     /locus_tag="IVK51_002743"
     CDS             1048..2439
                     /locus_tag="IVK51_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP6432277.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDNSEE
                     ALQEMNLVRTRMNKSEDIAQAFHVIHSFDKNISKELSIEKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNIDMETCKRLRIDPKDLERMRKINDEVLVENGLEPVDNQY
                     YENLEKRTNLYSNDPSKLKNGKINYQEFVQNAIEATLENCEVNSFVRFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRDELITLGREKQSRKEKSQYVERTFSARKLGK
                     KYSFESVFERLEKNLEETHMNNFVDLDTEQIIALASGVDYKTGELNVPFVELDSIAQK
                     QFSDHEGAKKIIKYDDFLREVDRHYANTISLEDDQEYTIKLHTDVNPRTLRSTKSKTI
                     LVKKTDQKELFLPAEGFKNCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQRRQRDFEMDF"
     gene            2655..2957
                     /locus_tag="IVK51_002744"
     CDS             2655..2957
                     /locus_tag="IVK51_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432278.1"
                     /translation="MSDPYIKVYCSKETKTIFDSFYEQNKGIFGTKANMFRVMVSNLP
                     VLANQSNVKLSNPESKGIEQKISEIESIVVGKLDEIDQKLSYYLKNKCKTEEGKDV"
     gene            2950..>3270
                     /locus_tag="IVK51_002745"
     CDS             2950..>3270
                     /locus_tag="IVK51_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432279.1"
                     /translation="MFKVYFPFQWLFKGILLLLLGLILFGSYLTIKARYFPEEKPVKI
                     VTKDLKMGETYQADGVEITFKKVELTEKRNEFAQHDRVLQVTYTMKNDTENPIRPQQW
                     SIENK"
BASE COUNT         1287 a          456 c          634 g          893 t
ORIGIN      
        1 gtcaaatcaa ccaagaacgg tagaagtacg tgaaacaaga gctgaagcaa gtcagggaga
       61 taagacgcaa aaaataaata ttatcgagga acgaaaagaa aggcctaacg aaaaagtgat
      121 tgtagaacaa gaagtgaagg agaagaaacg tccacgattt acttatgaaa ataatccact
      181 gtttcaggat atttctgata aaccaaatcc gctgtttgat agtctattta aaaaataaag
      241 aggtgattgt catgcaatta attctacaga ttagtacgca ggatttctta gaacttgccg
      301 aattatgcga ctcagtaaag aaagcgacaa atgaaaaaat cacggtcaca aattatattg
      361 aactgatgat tcaagaagaa ttaatgatga aaaatgaaat agagagcaat ttcaaatgtt
      421 gctctctaga aaaaatgtga ggggataatc aatggatgat aaacaaaaaa tgtacgaaaa
      481 aaatcgcaaa catagtgagg ccttaaattt tcgtgtcgat aaaaagactt atcttgaact
      541 gacattgatt gcaaaacaaa gaggaatttc tagagcagat gtttgtcgtg agcggttaaa
      601 gggactcaaa tatgaaccgc cgttattggc tgaagatgat agggtagctc taatcaaaca
      661 gttaaatcaa attggtaaca atttgaatca agcggtaaaa gcattaaata tcattgctaa
      721 atattttaga gaacaatctg aaaataagaa agaaaagagc ctgcatttta gttttgacgt
      781 tagccgttac aacattttta aagatgaact tgcgaatagc atagcaaaaa aaatgatgat
      841 tagttatcca gatgacattt ttttggagtc caatcggagt acatcttctt ttctcaaact
      901 gtcagaagta gaaaaagatt attttcaata ttggttagaa aagcaaacta gacccgatac
      961 gaaagaagag tattcccaaa aagttgaccg gaatttaatt cactcgattc aaattattgc
     1021 aagtttaaaa gatgaggtga aacaactttg ggagctacta taacatcgaa aggacgcaag
     1081 aaaaatttat cctcgctatt acaatatgca gctaatcctt caaaaaaaga aaaatgcgtt
     1141 gcagtctcag gaataaattg tagtgataat agtgaggaag cattgcaaga aatgaattta
     1201 gtaagaacca ggatgaataa atctgaagat atagcacaag catttcatgt tatccattcg
     1261 tttgataaga atatttctaa agagttaagt attgagaaaa tgcacgaaat agctgtcgaa
     1321 tttgcagaaa aagcttttcc aaattcgcaa attattgttg caagtcacaa tgataaagat
     1381 cattttcata gtcatcttgt tattaataat attgatatgg agacctgtaa gaggttgcgt
     1441 attgacccta aagatttaga acgaatgcga aaaattaacg atgaggtgct tgttgaaaat
     1501 ggactcgaac ctgtagacaa tcagtactat gaaaatttag aaaagagaac gaatctctat
     1561 tcaaacgatc caagtaaact gaaaaatgga aaaattaatt atcaagagtt tgtacaaaat
     1621 gcgattgaag ctacgttgga gaattgtgaa gtaaattcat ttgtgcgttt ttcggatcat
     1681 ttagcaaaag aatacaacat cgaagtttat caattttcaa aaacaaataa caaacttggc
     1741 tatgttcttt acaaacaaga tgtaaatttc tatgagcgta gagatgagct aattacttta
     1801 ggaagagaaa aacaatctag aaaagaaaaa agtcagtatg tcgaacgtac ttttagtgca
     1861 agaaagttag ggaaaaaata ttcatttgaa tctgtttttg aacgacttga aaagaattta
     1921 gaagaaacac atatgaataa ttttgttgat ctagatacgg aacaaattat agcgttagct
     1981 agtggagttg attataaaac tggggagtta aatgtaccgt ttgtggaatt agattccatt
     2041 gcacaaaagc agtttagtga tcatgagggt gctaaaaaaa tcattaaata tgatgatttc
     2101 ttgcgagaag tagacaggca ttatgcaaac acaatatctt tagaagatga tcaagaatac
     2161 acgattaaac ttcatactga tgtgaatcct agaactttac gtagtactaa aagtaaaaca
     2221 attttagtta aaaagacaga tcagaaagaa ttatttctac cagctgaggg ctttaaaaat
     2281 tgttctcata caccttctgc taaacagatt cactatcaaa tcagtcgcat aatgaatgac
     2341 gatgtgaaaa tgtatcgtgc taaaaaagat tacgagttca cgcaacaaat gattgaaaat
     2401 cagcaaagga gacaacgaga ttttgagatg gatttctaaa ggaattagcc agtagacttt
     2461 ccagtttccc atcgatacgg agggaattgg aaagaggcgg ttttgagggc atacgccagc
     2521 tacccatcta cttgccagtc aatccgccct tgcttacgca gggctacttt ctggcaagtt
     2581 agatggggaa aacctctgag ggagcaagct ccctacagac tccctaaata aaaaaagata
     2641 aggagtaaaa acacatgtct gatccgtata ttaaagtgta ttgttcaaaa gaaactaaaa
     2701 caatttttga cagtttttac gaacagaaca aagggatttt cggaacaaaa gccaatatgt
     2761 ttcgtgtgat ggtatcaaat ttaccagtgt tagctaacca atcaaatgta aaattaagta
     2821 atcctgaaag caaaggaatt gaacaaaaaa tttctgaaat agaatcaatt gtggtgggaa
     2881 agttggatga aattgatcaa aagttatcgt attatctaaa aaacaaatgc aaaacggagg
     2941 aggggaaaga tgtttaaagt gtattttccg tttcagtggc tctttaaagg aattctttta
     3001 ttgctactag gattgattct ttttggaagc tatctaacaa ttaaagccag atattttcca
     3061 gaagaaaaac cagtaaaaat tgtaactaaa gatttaaaaa tgggtgaaac ttatcaggca
     3121 gatggggtcg aaataacgtt taaaaaggtt gaattgaccg aaaaaagaaa tgaatttgcg
     3181 caacatgatc gtgtattaca agtgacctat acaatgaaaa atgatacaga aaatccgata
     3241 cgtccgcaac aatggtcgat tgaaaataaa
//
LOCUS       DACCDF010000154         3235 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCDF010000154 DACCDF010000000
VERSION     DACCDF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.162"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            184..1138
                     /locus_tag="IVK51_002746"
                     /pseudo
     CDS             184..1138
                     /locus_tag="IVK51_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 105 protein"
     gene            1155..2179
                     /locus_tag="IVK51_002747"
                     /pseudo
     CDS             1155..2179
                     /locus_tag="IVK51_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            2185..2664
                     /locus_tag="IVK51_002748"
     CDS             2185..2664
                     /locus_tag="IVK51_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP6432280.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            2680..>3235
                     /locus_tag="IVK51_002749"
     CDS             2680..>3235
                     /locus_tag="IVK51_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP6432281.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
BASE COUNT          980 a          540 c          751 g          964 t
ORIGIN      
        1 acttggaaaa cgttgtttct ccaagttctt tttttaattt ccaagtaact gaaagttact
       61 tttgttattt aacatttagg tcaattttag acagtttttg ttcaaaaagc gatatttata
      121 gtggacggta ttactgatat attaaggata taaaatgaaa gcgtttaaaa aggtggcgta
      181 aagatgatta ttaaagaaga gatttttgta cctaaagaat tagtgaaaga aaaaattgac
      241 ctgctcgttg aaaacctgac aacgatcaaa gacgataatg gtgaattttt gttgggcttt
      301 gatggactga aagtagatga caagagctgg acagtgtgga attggccaca aggtgtcggt
      361 ctatatggta tttataaaaa ttaccagatg acaaaaaatc ctcgcgctta tcaagtggtc
      421 aatgaatggt ttgaggatcg tatggaagaa ggcgctccgc ccaaaaacgt caatacaatg
      481 gcaccgctat tgactatggc ttacttgtat gaagacacga aagattcacg ctatttgcct
      541 tatttagaac aatgggcaga atgggtcatg aatgacatgc ctcggacaaa tgaagacggg
      601 ttacagcatg ccacatatgg accggaaaat aagaatcagt tgtgggacga tacattgatg
      661 atgacagtgc ttcctttggc aaaaatcggg aaactgctgg ttgacgattg ctattccaga
      721 gatcattgaa atcttagaat tatcgaaaga agatgcattg cgcaaattat tgatcgaaac
      781 acttgaagca caagtgcgtg cattaaaaac ttatcagtct gaaagcggtt tatggcatac
      841 gttgttggat gatccttctt cttacgtaga atcttctgca actgcaggat ttgcttacgg
      901 tattttgaaa gcggtccata aacgttattt accacaagaa tataaagaag tcgcttatcg
      961 tgctatccaa ggattgcttg aacaaatcga tgaaaaagga gaagttcaaa acgtttcaat
     1021 cgggacagga atgggggaca gtttagattt ttatcggaat atcggaatca cagcaatgcc
     1081 ttatggacag tcgttaacgg tgctttgttt gacagaattg ctcgtctctt attgttaaaa
     1141 gaaaggaaac ccatatgaaa aaaacatcac tgctgtttgc agcaagttcg cttagcctag
     1201 gtctgctagg tctaagcggc tgcggaaata attctagtgt ggcaactgtc gcagatgcag
     1261 acgtaaatga gatagttacc ttgcgctttg cttatgcaag caacagccag taattgatgc
     1321 aatgaatgaa tttgggcgtt tagtaaaaga aaaaacagat ggttccgtac agatccagta
     1381 tttcccagat ggacaactgg gtggagaacg tgaactgatc gaattgacac agtcaggagc
     1441 agtagatttc acaaaagtaa gtgcctcagc gttggaaagt ttctctaaag attattcgat
     1501 tttttccgtt ccttatctat tcaatgatga agaacatttc tttaaggtga tggataatga
     1561 agaaataatg gagccaatct accaatcgac caaaaattta ggatttaccg gattgaccta
     1621 ttatgattct ggtcagctca gcttttatat ggtagacggt ccgatccata cgccggaaga
     1681 tttgaaggga aagaagatcc gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct
     1741 tttaggcggt tcgcccgtac ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa
     1801 tttgatcaac ggatcagaaa acaatgaatt tgtcttgtat acagctggac atggcggggt
     1861 agctaaatac tattcctatg atgagcatac acgtgtgcca gatatcatca tcatgaatga
     1921 tgccatcaaa gaacagctaa ctgatgaaca ggaaaaagcc attgaagaag cagcgaaaga
     1981 atcaacggtt tttgaaattg aagcttttgc aaaagcaatt gaagaagaaa aagctttagc
     2041 acaagaagaa catggagtcc aattcaatga tgtagataat acaccgtttc gagaggcagt
     2101 tgccccactt catgatatat tcaaaaacga tcctacctac aaagaccttt atcaaaagat
     2161 ccaagagcaa ggagcgtagc tgtaatgaaa atgattttaa acaaggtatt agagatcctg
     2221 ggagctgttt tgcttgttgg gatggtcgct gttgttttat ggcaagtatt ttccagaacg
     2281 gttttaggaa atccaaatac ggtcacggaa gagcttgttc gattcggtct tgtctggttt
     2341 gccatgttgt cttccgcgta tgttgtggga caaaaagcac gtttagccgt aacggtttta
     2401 agtgaaaaat taactggcaa aaaaagtaaa attttagaag ttgttgttca aagtttattt
     2461 ctggtatttg ctgcgatgat catggtgtat ggcggatgga atgctgtcac attgacaatg
     2521 gggcagatct cgccttcttt aagtattcct atgggatatg tttatctgtc agtacctgta
     2581 tcgggggtac tcatcattat ctatagcctg atcaatctaa tcgatacatt aagcaacaag
     2641 ccagcaattc aagaaaaaat ataaaaatgg aggaataaaa tggcaataga agccggatta
     2701 gtgttatttt ttgtatttgt tgtgttgctt gtattaggga tgccgattgc tataagtatt
     2761 gcattctcat cgatgatgac tttattgtta gttataccgt ttgatgtttc tgcttttagt
     2821 tcagctcaaa aaatggtggg aagtatcaat agtttttcgc tgatcgctat tccgtttttc
     2881 gttctctcgg gcattatcat gaataatgga gggatagcta agaaactagt ggattttgcg
     2941 atgttgttcg tcgggcgcgt gccgggggct ttggctcata cgaatgtgtt agggaatgct
     3001 ttatttggtt ccatctctag ctcggccatt gccgcttcta ccgcgattgg tggagtgttg
     3061 atccctcagc aagtggaaga aggatatgac cggaagtttg cgacagcggt caatatcgcg
     3121 tcagctccga caggtatggt cattcctcca agtactgctt ttatcatgta ttcacttgtt
     3181 gctggtggtg cttcgatctc tgctttgttt atgggaggtt atctcgtagg cgctt
//
LOCUS       DACCDF010000155         3166 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCDF010000155 DACCDF010000000
VERSION     DACCDF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.163"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            50..1438
                     /locus_tag="IVK51_002750"
     CDS             50..1438
                     /locus_tag="IVK51_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAP6432282.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3166
                     /locus_tag="IVK51_002751"
     CDS             1776..>3166
                     /locus_tag="IVK51_002751"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP6432283.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIV"
BASE COUNT         1164 a          495 c          573 g          934 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtgga
//
LOCUS       DACCDF010000156         3076 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCDF010000156 DACCDF010000000
VERSION     DACCDF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.164"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(565..1131)
                     /locus_tag="IVK51_002752"
     CDS             complement(565..1131)
                     /locus_tag="IVK51_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP6432284.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1131..2087)
                     /locus_tag="IVK51_002753"
     CDS             complement(1131..2087)
                     /locus_tag="IVK51_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP6432285.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2087..2743)
                     /locus_tag="IVK51_002754"
     CDS             complement(2087..2743)
                     /locus_tag="IVK51_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP6432286.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT          979 a          593 c          544 g          960 t
ORIGIN      
        1 tttattttcg gaaaaagcta atatagctaa gattgaaaca tccatctgga atttatcttt
       61 tttactgctt tgatcataaa agtctgcata ttcatcactg atattttttc taataaattc
      121 ttccatcata aaaatcacct caaaacgatt atacgaactt acgttcttgt tgtaaagcga
      181 tatttgaggg gcaaaaaagg ggcataattt gactgatatt aagttagcta ttgatatatg
      241 aatgacatac aaaaatataa aatcttttaa aaatgcttat atatcaatgt ttttgccatt
      301 cttttatata tctgttatat gtcaaaaaaa tgtgttcaat ttcaatttgt atttgaataa
      361 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt
      421 ctcatgtttt tgatttggtt ggacttatta ccgcagatat aagcttttga gcaccgtgaa
      481 taaagaggtt atgaaatcaa cgctctaacc tgagtattag agaaaaaaac gcaaaacata
      541 tcatttatgt tttgcgtttt ttatttatct ttttttctca atacaatata aaataggcgg
      601 ctggttcttt tggttgataa accgataatt cagcacatta tatgtttcct gtggcagttt
      661 ggaacaaaaa gaatcaacca tatccagttc tttttctcca ccttcatgac catagtagac
      721 gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat atttcttcca tagctgtgcg
      781 tgtagtctct ggcatagtga tgattgcttt atcacttttt gggagatacc ctagattgaa
      841 aatccctgct ttgagatttt gttgttcagc tatcacttct cctaacgttt cgtggccttg
      901 tagaaacaat gagacacgtt cttctaactg acgctgttct agcctttcac gagtagcatc
      961 gattgcttgt tgctggatat caaaactata gacatgacca gtttttccga tgtgttctgc
     1021 tagaaaaacc gtatcgtggc cattgcccat cgttgcatct acgacgtgat cccctggttg
     1081 caagatttct tgcagtaatg tatggctgaa atgcaaagca gtttgcaaca tcagacgctc
     1141 acctttttcc gtacatcata ttttccttgg aagctatctc tgcgaagaag ttcttcatca
     1201 attgcattca agacttccca ttttttcaag ctccacattg gtccaatcaa ggaatcccac
     1261 ggcgcatccc ctgtcaaacg atggatgatg atctcttttg gtatcatttc cagctggtcg
     1321 caaatgacat tgacatagtc aggacggctc attaattgaa ggcgaccttc atgatagtcc
     1381 cgcaacattc tcgttttgcg catcagatgc atcaaatgga gcttgattcc ttggatatct
     1441 gaatctaaaa tggtgcgtct gacattttct ttcatcattt ctaaactttc gcctggtaat
     1501 ccgttgatca aatgcgtaca aacacggata ttatgtttgc gtaatttagc cacgccatct
     1561 aaataagttt gataatcatg tgctcggttg atcagttttg aagtttcttc aaaagttgtt
     1621 tgcaatccaa gttctaccca taaatacatc cgctcgttca gttcagcaag gtaatcaact
     1681 acttcatctg gcagacaatc cggtcgtgta ccgactgata atcctacgac acctggtaaa
     1741 ttgactactt gctcaaatcg ttctttgatt atttcaactg gcgcatgggt gtttgtaaaa
     1801 ttttggaaat aaacgatgta ttgttggaca tgaggccatt tttgatgcat catgtcgatt
     1861 tctttctgga attggattgg aagagggtct tcaggtgcaa cgatcatgtc tcccgaacca
     1921 gatacactgc aaaaagtaca accgccatgg gcaacagtcc catctcgatt cggacagtca
     1981 aatcctccat cgatcggcac cttgaaaatt ttcccgccaa actcatttcg taaagcgtaa
     2041 ttccaagaat gatatctttt atttccttct gaataaggaa aagtcattaa aagtccttct
     2101 ttctgtaaga gttatctatt tgttttcttt tttctctata cactggataa gtcaggcaca
     2161 gccaagaaag actgagtaag tagccgccga tgatatcagt aggaaaatga acgcccagat
     2221 aaatgcggct tatcccgaca cttactataa taaacatcat cagcaattga agcaatgtgc
     2281 gtaaggttct tttttgaaca aaagctggaa gcagaaatag taaagaaccg taaagaatca
     2341 tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt ttcaactact aagtgcgcca
     2401 atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg gttgatgatt ccagcaatta
     2461 caataaatcc agaaacgaac caaaatgttt ctgccctttt tttcttatac cacatccata
     2521 aagccgcaca agcaaataaa atgatgacgg taagaggatt accgaattta gtcataaaga
     2581 gttggtagct gttcagatca ggataagaag ctctgacaat gcctgtaaac cactgatcga
     2641 aaccagaaag ccacgacaga tggaacttta cgatccagcc caaaatgaaa aacaggacaa
     2701 gaaagaagct accagccacc tgaaatgatg ttttatattt catacaattc ctcctttgaa
     2761 gcaatgactt attatagcat ttttggtcca gcgaaaaaaa cggtaaagag ataaaaccaa
     2821 tgtatgattc ctctttgaat aaccaatatt cttataaaaa aaataactgt aatcggaaag
     2881 acattttttg gtaatgcaaa tgaaatgtta gggaaataaa atcagtgttt ttcgaaatcc
     2941 ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg aacagaagtt gttcattacg
     3001 gtaaattaca atatgattac aaaaaacaac aaagctctta aataactttt aataaatatt
     3061 aagagaagtt ataatt
//
LOCUS       DACCDF010000157         3020 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCDF010000157 DACCDF010000000
VERSION     DACCDF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.165"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1259
                     /locus_tag="IVK51_002755"
     CDS             <1..1259
                     /locus_tag="IVK51_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP6432287.1"
                     /translation="VIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGT
                     VDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEV
                     TDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKK
                     GTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDL
                     PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITE
                     GDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGN
                     TSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNI
                     FTISGGLILLGTLGLLGYKNRKKENE"
     gene            1393..2736
                     /locus_tag="IVK51_002756"
     CDS             1393..2736
                     /locus_tag="IVK51_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP6432288.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2848..>3020
                     /locus_tag="IVK51_002757"
     CDS             2848..>3020
                     /locus_tag="IVK51_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432289.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSEL"
BASE COUNT         1199 a          407 c          572 g          842 t
ORIGIN      
        1 gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt ccagtagaag
       61 tgacagacaa tcgttctgac gccgataaat atgaaccaac agtagaaggc gaaaaagtag
      121 aaattggagg cacagtagat ttaacagata atgttactaa cttgccagcg ttaccagaag
      181 gaacaacagt aacagatgtt actcccggcg gtacaattga taccaacaca ccaggcaatt
      241 atgaaggtgt cattgaagta acgtatccag acggtacgaa agatacggtg aaagttccag
      301 tagaagtgac agacaatcgt tctaatgcag ataaatacaa tcctgaagga caaaaagtga
      361 ctacagactt aaataaagag ccagatgcat ctgagggaat taagaacaaa gaagatttac
      421 caaaaggaac tacttatgct tggaaagaga aagtagatgt tagcacagct ggaaataaaa
      481 aaggtactat tgtagtgaca tatccagatg gatcatctga tgaagttgaa gtagacgtta
      541 cagtgacaga tactcgttcc gatgcagata aatataatcc tgaaggacaa aaagtgacta
      601 cagacttaaa taaagagcca gatgcatctg agggaattaa gaacaaagaa gatctaccaa
      661 aaggaactac ttatacttgg aaagaaaaag tagatgttag tacagctgga aacaaaaaag
      721 gtactgtagt agtaacatat ccagatggat ctaaagaaga agtagaagtt actatttctg
      781 tagtagataa aaaagcaccg aataaacctc aagttgatcc tattacagag ggtgaccaaa
      841 ttgttactgg taaaactgaa ccaaatgcag aggtgacagt tacattacca gacggaagtc
      901 aacaccatgg gacagctgat aaaaatggta attttacagt taaagttcct aaattagagg
      961 cagggacaaa agtgatagta actgcaactg atgaatctgg gaatactagt gaacctacta
     1021 atgtagttgt ttcttccaac aaaaaagaca gtggaaaaaa cggtagtaaa ggtagtaaga
     1081 ctgacaatca aggtagcaac agcaatcaag acaaaaatag aggcaaaagc cagtcctcaa
     1141 aagtttttcc taagacggga gagagtgaca gtaatatctt tactattagc ggtggtttaa
     1201 tactgttagg aacgttagga ttattagggt ataaaaatcg aaaaaaagag aatgaataga
     1261 aaatcaattt cttaagtctt ctctgagaag aggaagaatc tttttcttcc tctttttgct
     1321 tttttatgat gggaaataag tgttagttaa agatgaatat actttttata gaaaggaaaa
     1381 gaatgtaatt aagtggaaaa aatagtaatt attggttcaa accattcagg gctagccgca
     1441 gctcgatttc taaacttaag cgattcaaat tatgaagtta ttctaatcga taaaaaatca
     1501 gaaattagtt acttggaatg tgggacatcg ttatttataa aaggagaagt aagtaattta
     1561 gaagatcttt tttatcaaaa tagaatgaca ctaaattctg agataaaaaa gttatttcta
     1621 gagacagaag ttgacaaaat tgatattcaa aataaaaaga ttagttgtgt aaaaaaagat
     1681 ggtacaagat tctctcaaga atatgacaaa gttatattag ctacaggttc aaagcaaatt
     1741 atgctagata taccggggaa taatttagct aatatttttt cgataaagaa ttttccaaat
     1801 gcaaaagaga tttttaaagc tttggatgat agtactatta aaaaagtagg aattattgga
     1861 gcaggatata ttggagttga aattgctgaa gccataagaa aaagagggaa agaagtatat
     1921 cttttcgatg ttgcagatag agtgttgtcg acttattacg accgtagttt ttcagataaa
     1981 gttgaagaaa tactatcaaa aaatggaata catctttgtc tatcagaaag tcctaaaaaa
     2041 tatttaggaa gaaaaaagat tgaaaaaata gtcactacaa gtggtaccca atactctatg
     2101 gatatggtcg tacaagctat aggatttcta tctaatagtc caattgggga aaaggaactc
     2161 ttgagagatg ttagtggagc atatctaact aatgaatacc agcaaacaaa tataaaagat
     2221 atatatgcta ttggggattg tgctacaact ttttttacgt ccattaatag aagtagtgtt
     2281 gatttttcta tttctaatgc tctaagaaca gcttatattg caactcaaca tattaacaat
     2341 caagatttta caccttctgg ttcccaattt acaaatggct tttctatatt taattacaat
     2401 ttttacgcag caggtttgaa tttaaaaaca gcacgattat taagtgagtc tatagattat
     2461 atagaaatag aagatttcat tagacctgct tttttgagaa aaaatagtcg tgtaaaatta
     2521 agaattagct ttgaaaaaaa tacaaagaga atagtaggtg tacaactttg tagtcaagaa
     2581 gatttatctg gactattatc aatgttttct cttgctatag aagaacaaat tacattagac
     2641 aaattgaaaa tgttagatta tttatttcat cctagtttta gccaacccta taattttatt
     2701 ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga aatcaagaaa
     2761 tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag aaaacactat
     2821 taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt
     2881 atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca
     2941 acatacaaaa ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc cgatgtaaca
     3001 gcagagagta gtgaactttt
//
LOCUS       DACCDF010000158         2924 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCDF010000158 DACCDF010000000
VERSION     DACCDF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.167"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1367..1885)
                     /locus_tag="IVK51_002758"
     CDS             complement(1367..1885)
                     /locus_tag="IVK51_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP6432290.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          968 a          529 c          414 g         1013 t
ORIGIN      
        1 gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt tggcaataat
       61 tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga ttgcaaaaaa
      121 catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag gccggggtag
      181 cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt tcaatatatt
      241 gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt aaaccagata
      301 taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt ggattaagat
      361 aacgactatt tattttatca tatttttact ataccctata cttttacttg gtatatttat
      421 gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat aaaaatacta
      481 aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt atttagataa
      541 cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct actcaatcga
      601 aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag accttttaca
      661 tcttgcagaa atcggtactg ggattaaact aagcaataaa aactaatgtt cccctcattc
      721 tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac cttcaggatc
      781 ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag gataggcgat
      841 aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg tatgttggct
      901 ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa tcgttcttaa
      961 caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt gccttaccct
     1021 tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc ggcggtctga
     1081 aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc ctttgtagcg
     1141 gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt tggccaacca
     1201 aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc acttgcattc
     1261 acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct tactgttaac
     1321 atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac aaagctaatc
     1381 tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta tttacatgaa
     1441 taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat tttcttttgt
     1501 gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata tcatcaatat
     1561 tacgattgag aatttcctct aattctttca atggtagatg tgcataggta tttgtaaacc
     1621 acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt ttaacagata
     1681 atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca acttgatatg
     1741 ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc aattcttcag
     1801 gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct ttttttaatt
     1861 cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat tgtctccttt
     1921 aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat atctgatatg
     1981 cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc tcatctaaag
     2041 aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg tagtaaaatc
     2101 ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact actatcaatt
     2161 actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa gtgaggatag
     2221 ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc tttatatagg
     2281 attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg aagtaaaaat
     2341 tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac gaaatgatca
     2401 actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg ctcgacgaac
     2461 tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag ctttttatgt
     2521 tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc tagggtgcgg
     2581 tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta aaattatgaa
     2641 taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct ggtgttatag
     2701 attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa accgatactc
     2761 tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa acttcactaa
     2821 cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt atcaaaaatg
     2881 ctgctcattt caacttatat gttttagtga actaccccca ctta
//
LOCUS       DACCDF010000159         2917 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCDF010000159 DACCDF010000000
VERSION     DACCDF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.168"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            204..1172
                     /locus_tag="IVK51_002759"
     CDS             204..1172
                     /locus_tag="IVK51_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6432291.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1165..2196
                     /gene="vanA"
                     /locus_tag="IVK51_002760"
     CDS             1165..2196
                     /gene="vanA"
                     /locus_tag="IVK51_002760"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6432292.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2202..2810
                     /locus_tag="IVK51_002761"
     CDS             2202..2810
                     /locus_tag="IVK51_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6432293.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          787 t
ORIGIN      
        1 aattttttag gaaaatctca aggttatctt tactttttct taggaaatta acaatttaat
       61 attaagaaac ggctcgttct tacacggtag acttaatacc gtaagaacga gccgttttcg
      121 ttcttcagag aaagatttga caagattacc attggcatcc ccgttttatt tggtgccttt
      181 cacagaaagg gttggtctta attatgaata acatcggcat tactgtttat ggatgtgagc
      241 aggatgaggc agatgcattc catgctcttt cgcctcgctt tggcgttatg gcaacgataa
      301 ttaacgccaa cgtgtcggaa tccaacgcca aatccgcgcc tttcaatcaa tgtatcagtg
      361 tgggacataa atcagagatt tccgcctcta ttcttcttgc gctgaagaga gccggtgtga
      421 aatatatttc tacccgaagc atcggctgca atcatataga tacaactgct gctaagagaa
      481 tgggcatcac tgtcgacaat gtggcgtact cgccggatag cgttgccgat tatactatga
      541 tgctaattct tatggcagta cgcaacgtaa aatcgattgt gcgctctgtg gaaaaacatg
      601 atttcaggtt ggacagcgac cgtggcaagg tactcagcga catgacagtt ggtgtggtgg
      661 gaacgggcca gataggcaaa gcggttattg agcggctgcg aggatttgga tgtaaagtgt
      721 tggcttatag tcgcagccga agtatagagg taaactatgt accgtttgat gagttgctgc
      781 aaaatagcga tatcgttacg cttcatgtgc cgctcaatac ggatacgcac tatattatca
      841 gccacgaaca aatacagaga atgaagcaag gagcatttct tatcaatact gggcgcggtc
      901 cacttgtaga tacctatgag ttggttaaag cattagaaaa cgggaaactg ggcggtgccg
      961 cattggatgt attggaagga gaggaagagt ttttctactc tgattgcacc caaaaaccaa
     1021 ttgataatca atttttactt aaacttcaaa gaatgcctaa cgtgataatc acaccgcata
     1081 cggcctatta taccgagcaa gcgttgcgtg ataccgttga aaaaaccatt aaaaactgtt
     1141 tggattttga aaggagacag gagcatgaat agaataaaag ttgcaatact gtttgggggt
     1201 tgctcagagg agcatgacgt atcggtaaaa tctgcaatag agatagccgc taacattaat
     1261 aaagaaaaat acgagccgtt atacattgga attacgaaat ctggtgtatg gaaaatgtgc
     1321 gaaaaacctt gcgcggaatg ggaaaacgac aattgctatt cagctgtact ctcgccggat
     1381 aaaaaaatgc acggattact tgttaaaaag aaccatgaat atgaaatcaa ccatgttgat
     1441 gtagcatttt cagctttgca tggcaagtca ggtgaagatg gatccataca aggtctgttt
     1501 gaattgtccg gtatcccttt tgtaggctgc gatattcaaa gctcagcaat ttgtatggac
     1561 aaatcgttga catacatcgt tgcgaaaaat gctgggatag ctactcccgc cttttgggtt
     1621 attaataaag atgataggcc ggtggcagct acgtttacct atcctgtttt tgttaagccg
     1681 gcgcgttcag gctcatcctt cggtgtgaaa aaagtcaata gcgcggacga attggactac
     1741 gcaattgaat cggcaagaca atatgacagc aaaatcttaa ttgagcaggc tgtttcgggc
     1801 tgtgaggtcg gttgtgcggt attgggaaac agtgccgcgt tagctgttgg cgaggtggac
     1861 caaatcaggc tgcagtacgg aatctttcgt attcatcagg aagtcgagcc ggaaaaaggc
     1921 tctgaaaacg cagttataac cgttcccgca gacctttcag cagaggagcg aggacggata
     1981 caggaaacgg caaaaaaaat atataaagcg ctcggctgta gaggtctagc ccgtgtggat
     2041 atgtttttac aagataacgg ccgcattgta ctgaacgaag tcaatactct gcccggtttc
     2101 acgtcataca gtcgttatcc ccgtatgatg gccgctgcag gtattgcact tcccgaactg
     2161 attgaccgct tgatcgtatt agcgttaaag gggtgataag catggaaata ggatttactt
     2221 ttttagatga aatagtacac ggtgttcgtt gggacgctaa atatgccact tgggataatt
     2281 tcaccggaaa accggttgac ggttatgaag taaatcgcat tgtagggaca tacgagttgg
     2341 ctgaatcgct tttgaaggca aaagaactgg ctgctaccca agggtacgga ttgcttctat
     2401 gggacggtta ccgtcctaat cgtgctgtaa actgttttat gcaatgggct gcacagccgg
     2461 aaaataacct gacaaaggaa agttattatc ccaatattga ccgaactgag atgatttcaa
     2521 aaggatacgt ggcttcaaaa tcaagccata gccgcggcag tgccattgat cttacgcttt
     2581 atcgattaga cacgggtgag cttgtaccaa tggggagccg atttgatttt atggatgaac
     2641 gctctcatca tgcggcaaat ggaatatcat gcaatgaagc gcaaaatcgc agacgtttgc
     2701 gctccatcat ggaaaacagt gggtttgaag catatagcct cgaatggtgg cactatgtat
     2761 taagagacga accatacccc aatagctatt ttgatttccc cgttaaataa acttttaacc
     2821 gttgcacgga caaactatat aagctaactc tttcggcagg aaacccgacg tatgtaactg
     2881 gttcttaggg aatttatata tagtagatag tattgaa
//
LOCUS       DACCDF010000160         2862 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCDF010000160 DACCDF010000000
VERSION     DACCDF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.169"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            5..2377
                     /locus_tag="IVK51_002762"
     CDS             5..2377
                     /locus_tag="IVK51_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432294.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2438..>2862
                     /locus_tag="IVK51_002763"
     CDS             2438..>2862
                     /locus_tag="IVK51_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432295.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYA"
BASE COUNT         1144 a          455 c          621 g          642 t
ORIGIN      
        1 ggacatggca gaagaaaaag aaccactaga caatagtcgc ttgggaaaat ccaaaagaaa
       61 attagtacga ttgcaaaatg aattaaacga acaaattgag aagatgtttg aacaccagag
      121 aaagaccaac ggtcagccaa tgaacgataa gagaaatggg cattcttggt ttaggcaaca
      181 agaacgttta gaaaacaaag tgcatagtct gagagaagag atcaagcaac aagaaaaaca
      241 ggtagaaaaa cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 cctggatatg acaatcgaaa atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg actttttcag ccgctacgat ccgaaaatac caacggaaat tggaaacatt
      421 agaacagctg aaagaaagat cagaaaaagg aaaagaaaat cttcttcctg aagtacaagc
      481 aattattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 agactatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgc
      661 gccaactgat tctttgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tagaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 aaaggaacaa aaacaaacgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaagcaaga
     1081 agtcaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtgaaa gaacttttga attttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ctgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaagga ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actacacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgggg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cttcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 ggcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctgtagaaac ggcagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtga ttttaaaagg
     2221 aaaaagttat ggaaaagaga ttcaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg aaacagtgtc agaagtggcc aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg cgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg tttgaaaatg accgaaaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggctgt
     2521 agtcaataaa ggacgagact atcgcttaga agaccatgat tcatttgttt ttgatcgaag
     2581 aaaacaacgt ttttattgga acagtcaaaa tattagtggc gacattattg aattagctaa
     2641 actttttttc atagacaaag agatccaaga ctctaagcaa caatttaaag cagccacaga
     2701 ttttattcta aagaatgaag acaaaacaga acgagtagaa aatcttcatt ttgagacaga
     2761 aaagtacaaa gatcaccccg ttgattatca accactcaca gaaaaaggaa ggaattatct
     2821 gaaggaagaa cgaaaactac cagagtggct gattgattat gc
//
LOCUS       DACCDF010000161         2860 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCDF010000161 DACCDF010000000
VERSION     DACCDF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.170"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..420
                     /locus_tag="IVK51_002764"
     CDS             <1..420
                     /locus_tag="IVK51_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432296.1"
                     /translation="EGVTGRKISLKDESSTTVSDANILANIILDFIFEHDIPFKNGYE
                     ILPQNEQYFFYKCLTNRVCCSCGKKNADIHHVDSVGMGNNRKKINNSGRRFMALCREC
                     HTKIHVEGFTTFTTKRKLTAVVLKDSDLKRLGLNIIE"
     gene            428..943
                     /locus_tag="IVK51_002765"
     CDS             428..943
                     /locus_tag="IVK51_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP6432297.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1052..1903
                     /locus_tag="IVK51_002766"
     CDS             1052..1903
                     /locus_tag="IVK51_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP6432298.1"
                     /translation="MADNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIEVLDNGAIYMSDI
                     QSFIGKSSTEADRKRKYRKEIEEAKQNLITSGQMSDKCPDKTTPELEIELEKDIELEK
                     DIENVTPPKKSKAKPIRHKYGEYKNVLLSDEQMEKLKTEFPNDYQERIERLSEYCESS
                     GKTYKNYLATIRSWARKEKSEPKNASGAYKRTGRREKLPEWAIDQEAYQKKKALERAN
                     RQSKAPF"
     gene            1915..2184
                     /locus_tag="IVK51_002767"
     CDS             1915..2184
                     /locus_tag="IVK51_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432299.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFADCVKGLQKHLSELEQV"
     gene            2346..2648
                     /locus_tag="IVK51_002768"
     CDS             2346..2648
                     /locus_tag="IVK51_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432300.1"
                     /translation="MNKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEGSS"
     gene            2645..2806
                     /locus_tag="IVK51_002769"
     CDS             2645..2806
                     /locus_tag="IVK51_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6432301.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
BASE COUNT         1103 a          437 c          627 g          693 t
ORIGIN      
        1 gaaggtgtca cagggcgcaa aataagcctc aaagatgaat cgagcacaac cgtaagtgat
       61 gccaatatct tagcgaatat catcctagat ttcatctttg agcatgatat tccgtttaaa
      121 aatggatatg aaattttgcc ccaaaacgaa cagtatttct tttacaaatg cctcacgaac
      181 agggtgtgct gtagttgcgg aaagaaaaat gctgatatac atcacgtaga ctctgtcggc
      241 atgggaaata accgaaaaaa aataaacaat tctggtaggc gctttatggc tctgtgcaga
      301 gaatgccata cgaaaattca tgtcgaaggt tttactacat ttacaactaa aagaaagctt
      361 acggcagttg ttctcaaaga cagtgattta aaaagattgg ggttgaatat cattgaatga
      421 attatggatg gatatcgaag gatacgaggg agtatatcaa gttagtagct taggaagagt
      481 taaatctaat cgccctaact atgtaattaa taagggatac gttaacaaag aaggatttat
      541 tatgaaacca tctgacaatg ggaaagggta tcaaattatc ttcttaagcg gcaaaggttt
      601 tagagataga aggtatgtcc atcggctagt agcttttcac tttttaaaag aagcgtattt
      661 cgaaggtgct gaagtcaatc ataagaacgg agataaatct aacaatactg ttgaaaacct
      721 agaatggctc agttctgatg ataataaaaa gcatgctaga aaaaatggat taactaactt
      781 gaaaggacca tcaaaactaa ctgatttgca agctttagct atcaagcgtt tatatacaaa
      841 caaattgatg agctccggag aaataatgaa actttttaat gtcagcaggc ataccgtgct
      901 gaatatagct tcagggaaga catttagttt cttggaagat taagagaaac acaaaatagg
      961 tgtgactaat ttcaaaaata agtatcaaat caaagggatt aagttaaacc aggaaacaat
     1021 taagaaactt agaataggag gataaaaaat attggctgac aacaaacgat actactattt
     1081 aaaactaaaa gagaacttct tcgatagcga cgagatggtt cttttagaaa gtatgccaga
     1141 tggatacatt tattctaata ttcttctcaa actttattta agaagtctga aacatgaagg
     1201 caaactaatg tttaatgaca ggattccatt caattctaca atgcttgcga ctattacaag
     1261 acactctgtg ggagttgtag aaaaagcagt acaaatattt cgtgatttac aacttattga
     1321 ggtattagat aacggagcaa tttatatgtc tgatatacaa agttttattg ggaaatcttc
     1381 aactgaagct gataggaaga gaaaatacag aaaagaaatt gaggaagcaa aacagaattt
     1441 aataacaagt ggacaaatgt cggacaaatg tccggacaaa actacaccag agttagagat
     1501 agagttagag aaagatatag agttagagaa agatatagag aatgtaacgc ctccgaaaaa
     1561 atcgaaggct aagcccatcc gtcataaata cggagagtat aaaaatgttc ttttgtcaga
     1621 tgagcagatg gagaaactca aaacagaatt ccctaatgac taccaagagc gaatagaacg
     1681 gctatctgag tattgtgaat catcaggtaa gacttataaa aactatttgg caactattcg
     1741 aagttgggca aggaaagaaa aaagtgagcc taagaatgca agtggtgcat acaaacgcac
     1801 aggacgacga gagaagcttc cagaatgggc aatcgaccaa gaagcctatc aaaagaaaaa
     1861 agcgctagaa cgagctaata gacaatcaaa agcaccattc taagaggtgg aaaattgaaa
     1921 atcgattatc tagaactgat caatgaaata gcaagttaca aaactggtga ggaaatagag
     1981 attctgagag acgtttatga acaacttgat gaagctggaa tcgaacgaat taagaatgat
     2041 cgttcaagtt ggagtaaact cagatactat ttcgcacttt atatcgatgc aacacaatta
     2101 agaaatttag cttatacaaa attactattt gctgattgcg ttaaaggatt gcaaaaacat
     2161 cttagtgaac ttgagcaggt gtaatcagat ggatctaaaa acatttacag cacagatcga
     2221 actaatgcat caagaagctt taagacaaag cgcctcgtac gaagacaagt ggctcaacac
     2281 gttccatggt ggacgtgaga gcgcacttga tcaagtgctc aaattattga aaggagaatg
     2341 tcgggatgaa taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
     2401 ggcaaaatga caaagaagct gcttcagaag tgatgagact tggaagagcg atgtgggcag
     2461 acaagagcaa caagccaaga ccgcgaaaaa tcgcaatttg gcacggtgac aaacttctag
     2521 tgacagggac agctgaacag ttagcaagtc tcacaggctt gcacgagaaa atcgtgagaa
     2581 aaagagctag gtgtggatac acagacgtta agaagagaac gtttagatac gtggagggat
     2641 cgtcatgaca acagaagaag tgattcaaat gcgtattcga aacattcagc gtgaaattga
     2701 cgatctagaa cgaacaaagg cagtgatggt caatgaaacg gctagaaagg caatcgattt
     2761 gcacatagag aacttaagaa gggaaattcg gagattggag gaatgagcgt ggataagaaa
     2821 gcagcaatga aacgaatcat cgaactgaca cattctgaga
//
LOCUS       DACCDF010000162         2778 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCDF010000162 DACCDF010000000
VERSION     DACCDF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.171"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..197
                     /locus_tag="IVK51_002770"
     CDS             <1..197
                     /locus_tag="IVK51_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432302.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IVK51_002771"
     CDS             314..811
                     /locus_tag="IVK51_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6432303.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IVK51_002772"
     CDS             898..1290
                     /locus_tag="IVK51_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6432304.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="IVK51_002773"
     CDS             1274..>2778
                     /locus_tag="IVK51_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP6432305.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DACCDF010000163         2775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCDF010000163 DACCDF010000000
VERSION     DACCDF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.172"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..129
                     /locus_tag="IVK51_002774"
     CDS             <1..129
                     /locus_tag="IVK51_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6432306.1"
                     /translation="SGITMEKTKKGLLKQWDRKSHELFISILKELEEHENWYAEVE"
     gene            130..678
                     /locus_tag="IVK51_002775"
     CDS             130..678
                     /locus_tag="IVK51_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432307.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            938..1666
                     /locus_tag="IVK51_002776"
     CDS             938..1666
                     /locus_tag="IVK51_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP6432308.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWCKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLGYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            1696..>2775
                     /locus_tag="IVK51_002777"
     CDS             1696..>2775
                     /locus_tag="IVK51_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HAP6432309.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEKAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYRKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAIDTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIE"
BASE COUNT         1010 a          371 c          593 g          801 t
ORIGIN      
        1 tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa aacaatggga tcgcaaaagc
       61 cacgaattat ttatcagtat tttgaaagaa ttggaagaac acgagaattg gtatgcggag
      121 gtggagtaat tggctgacat caaaggactt gatatttttg ggaacaacaa aggactcgcc
      181 aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag caattacacc atctgaaaat
      241 aagcaattag aagtgcacca agttcaagaa ataaaaactt cctccaatgt tgaaagacag
      301 agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa agtttgataa ggtttattcc
      361 accaagcatc ctttaaaact gtcgactttg ttaaatgcga cttccagagt tttagtagaa
      421 aagtatgaga ctaatatgac aagagatgaa ttgcttagaa aagctttaga tgagtatatt
      481 aaacagaatc ttacgcaaga agataagcaa gatttgttta atgacgtggt gaaagaacta
      541 gacttgttca gagaaaaata tccaattgtt ccagaattag atgatcgtgg agaaataata
      601 agaactacca gtcagataaa acaagatacc gaagactcac tgcgcaaagg gtggggaatg
      661 aataataaag ttagataata atcaaagaag aactaagtaa ctacctttaa acggtagtta
      721 cttagttctt ctttgatttg cgtgaaatag aggggattta tttttctggt tacgtgatta
      781 aaaaatccaa aaaatgagca taggaacggt tatgatttta gggtgtgttt taatgagata
      841 ttgtgatttc attaacaagt gtatgataat ctgttgttga gttagtaaat aagtaataga
      901 gctaactcta aaataaaact attaggagga gatgtaggtg aaaaagttta aaaagaaaat
      961 atatttagga ttggttgttg gggtgagtat gtttgggttc atggctcata ctaaacaagt
     1021 aactgcagat actaaccaaa aagaaaggat tatgagccaa ttagatgact ctcaaataga
     1081 gtatgtaccc aatgagctaa ttgtcagttt aaaggctaca caaagaattt caacagagaa
     1141 aaataaaccg caaaatttat tgggtgtaaa agttatggtg gaaaaagatt tgactcttag
     1201 gacaaatgaa agcgattata ataagcgaga tcaagtatta ttagtgaaat tgaacgaaga
     1261 aattagtgtg gaagaggcag taagaacatt taatgcttct gatatggtca attatgcaga
     1321 gccaaactat gtggttgaac tagagccgtt gaatgatgtt gaggagccaa atacaactga
     1381 ttctttagga aatcgatcaa ttattcttaa cgatcctaat tggtgtaaac aagctaattt
     1441 aagagagatt agcatgccat ccgcttggga agaaaccaag ggttctaagg atgtgaaggt
     1501 agcggtcatt gattcaggaa ttgattatca acatgaagat ctttctggaa atgtagatga
     1561 aagtttggga tacgattttt ttgacgatga tacagatcct atggatacta tgggacatgg
     1621 acacatattg ccgggattat tggtgctcaa acaaacaacg aattaggtgt tgctggaata
     1681 aattgggatg ttacgatggt acctatacgt gtatttggta gtagtggagg taccagtttg
     1741 gcaatcttaa ctgaagctat taattatgca tcccaaaaag ggtttccaat tatcaatttt
     1801 agtataggta gtgttgttta tagcagtaca gaaaaagcaa ttaaaaacta tcctggatta
     1861 ttcgtagcag cagctggaaa taatgggata gatatgactg ataaaccttt ttacccagct
     1921 tctttggata tacctaatat tattactgta ggaaattcta gtggttatga acgaagttct
     1981 gattcaaatt ataggaaaac aggtgttgat ttattcgcta gagggtttcg tgtttttagt
     2041 acatttctag gaagtaaata cacgtatatg tcaggtacct cgatggcaac acctcatgtc
     2101 actggtagtg ctgcattagc gttgtcacta gatcctgatc tatctactga tgaattaaaa
     2161 aaaatcattt taaatagtgt tgattcattt tatgaactat ctgatttatg tcttacaggg
     2221 ggaagactaa atacgaataa tgtcgtacga caagtaacgg gtaaacttac tgcatctaat
     2281 atcacattaa gattaaatag tgaatggtca gatgaaattg ctaaagagat gtgtcacgtt
     2341 cactggattg accagtcagg aaatgatttg acggatcaag tggttgttct aaaaaacgaa
     2401 gtggatacat ctaactttgg cgattacgaa gtagtttttg catcacctga tttaacagat
     2461 caaatctcag ttacagtgac cgtacctcga ttacgtaaac ttacgctagg aaaagaaaca
     2521 gcaaccattg atattggttc cgcatgggat agtgtcatag ccattgatac ttttcaagtg
     2581 aaagctatag atgattttgg gaatgatgtt acaaatcaag tacaggttac tattaatgct
     2641 gatatagata caattaattc aattcaagca gtgattaagt ttgaagttcc agaattaggc
     2701 ttagcccaaa ttggtagact gaatattact gcaaaaaata gtacaatcaa tggattgaat
     2761 gaagttagca tcgag
//
LOCUS       DACCDF010000164         2719 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCDF010000164 DACCDF010000000
VERSION     DACCDF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.173"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IVK51_002778"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IVK51_002778"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6432310.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1064 a          421 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tatcaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatca
//
LOCUS       DACCDF010000165         2650 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCDF010000165 DACCDF010000000
VERSION     DACCDF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.174"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            36..197
                     /locus_tag="IVK51_002779"
     CDS             36..197
                     /locus_tag="IVK51_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432311.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            210..713
                     /locus_tag="IVK51_002780"
     CDS             210..713
                     /locus_tag="IVK51_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP6432312.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            773..1162
                     /locus_tag="IVK51_002781"
     CDS             773..1162
                     /locus_tag="IVK51_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6432313.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1149..>2650
                     /locus_tag="IVK51_002782"
     CDS             1149..>2650
                     /locus_tag="IVK51_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HAP6432314.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKG"
BASE COUNT          788 a          439 c          610 g          813 t
ORIGIN      
        1 aatggttttt aaccaatgcc caatccttag tcttactggc aattgtggtc attggtttgt
       61 ttttaggatt taagcgggag ttttccaaac tgattggctt tttggtgatt gctttgattg
      121 cggtcggatt ggtctttaat gcttctgggg tgaaggatgt cttactcagt ttgtttaacc
      181 gaattgtcgg tgcgtaaggg ggaagcacga tggaacagat gcgtgtttat attgcgaacc
      241 tcgggaaata taacgaaggt gaattggtgg gggcgtggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacgaatt gggttaaatg acgaatacga ggaatatgca attcatgatt
      361 atgaacttcc ctttgagatt gatgagtata cgccaattga ggaaattaat cggttgtgtg
      421 gcttagcgga ggaacttgaa ggaacgccag ttggtgaagt tgcttctgag attcaacatg
      481 ctttcttcaa ttcttttgag gagatggtag agcatgtgga tgatattatc tattatccag
      541 attgcgatga tatgagtgat gtagcatatt tcttgattgt agaagctggt gattttggag
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtaga gacttggaga
      661 ttggaggcaa ttatttggta acgagtcgag gtgtttttga gtatccatat taacaaaatt
      721 attgaagggt agttgatttc taaactatcc ttctttttaa aggaatgatt tgatgaagaa
      781 aataaaaagc tacaccagta tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt
      841 tcaactgcct tttcctgtga cttttaacca gatggcttgg tttgttttat ccttgctgtt
      901 tgtcatcgta tttgcccatg tgcctccact atctatgata gagggggcct ttttaaaata
      961 cttaggtata ccggtggcag ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa
     1021 accattggga tttttacgat cttttattag ctatcactta cgatttaagg tcactttcgc
     1081 aggaaagaaa gtcaaagaac agaagaaacg atgggacgaa cccattactc tggtaaggag
     1141 tgtgaactat gtacccgatt aagtatattg aaaataatct tgtctttaat caagagggag
     1201 aatgttttgc ctactatgag ttaatacctt acaattactc ctttttgtca cctgaacaga
     1261 aatatcaagt ccacgataac tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc
     1321 atgccttaca aattgccaca gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg
     1381 aaattaccgg gcgattaaca gaagtagcga aacaacgaat tgatttacag acagaggcat
     1441 tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc ttcaaactga
     1501 ttgcgacaga tgaagaggtc aatctaaaga atctcaagaa atctttcttc tccggctttc
     1561 aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg
     1621 aagaaattcg ccgatatacc aagcttgaaa aactaatgga aagtaaattg gctcgaagat
     1681 ttaaagtcag acgagtcaca ccgagtgatc tgatgtattt gattgaacat atctatggcg
     1741 aaaagggtac accatttgaa gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg
     1801 aaactttggt gaaacgttac gacttattac gtcccagtcg ttgtttgatt gaagaaaagc
     1861 cccggtactt atgcatggaa catgagaatc atgaatcata tgtagcgtac ttaacaatta
     1921 atacaattgt gggggaaatg gagtttccct cctcagaact tttctattac cagcaacaac
     1981 aatttacatt cccaattgac actagtatga atgtggagat tgtgacgaac aaaaaggcac
     2041 ttgctaccgt tcgcaataag aaaaaggaat taaaagactt ggacaaccat gcctatcaat
     2101 ctgacaatga aacaaattcc aatgtcttag atgcattgga ttccgtagat gaattggaaa
     2161 ctaccttgga tcagtccaaa gaatccatgt acaaactgag ctatgtggtt cgagtaagtg
     2221 ccgaatcagt agatgaacta aagcgccggt gtgatgaagt gctagatttt tatgatgata
     2281 ccaatgtaaa actagtccgg ccttttggcg atatgatggg gctacatgaa gaattcttac
     2341 cttctagtaa gcgatacatg aatgattata tccagtatgt tacgtctgat tttctcgccg
     2401 gacttggttt tggcgccact cagatgttag gggagttgga aggcatttac tttggttata
     2461 acgtagatac ggggcgcaat gtttacctga aaccggcatt ggcctcacaa ggggtcaagg
     2521 gttcagttac caatgcgcta gcagcggcat tcctcggttc actcggtggc ggaaagtcct
     2581 ttagtaacaa tctcttagtt tattacgccg tcctttttgg tggacaagct gtgattgtcg
     2641 atccaaaagg
//
LOCUS       DACCDF010000166         2648 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCDF010000166 DACCDF010000000
VERSION     DACCDF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.175"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            706..2565
                     /gene="ltrA"
                     /locus_tag="IVK51_002783"
     CDS             706..2565
                     /gene="ltrA"
                     /locus_tag="IVK51_002783"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6432315.1"
                     /translation="MNKVKTNKTLRHSEYYGMTQIFDELYVKSDKKEVFTELMTIIMS
                     DENIRLAYRNIKNNKGSYTASTDKKSIRDIKTLSEDTFLTEVKVLFRCYKPRAVRRVE
                     IPKPNGKTRPLGIPSIWDRLIQQCILQVLEPICEAKFHNYSYGFRPNRSTHHAIATCN
                     RLANRGKLHFVVDVDIKGFFDNVNHSKLLRQMWTIGIRDKWLLGIIRSMLNAPIVLPT
                     GKVEHPKKGVPQGGVLSPLLSNIILNELDWWVASQWQNTTIETEKIMDRRDKGKGIDK
                     TNQYRALRRTNLKEIYIVRYADDFKIFCRKRSHADKIFHATKMWLKERLSLEISEEKS
                     KIVNLKKGKSEYLGFELKLIKKGKKYVVESHMSKKSMTRVKIQLKKQLRKVARPKNHC
                     EQAKEIGLYNSMVIGIHQYYGIATCVNLDCSKIAYTLKPFINHKLPTKKHGKILNTFI
                     KSKYGKSKEMRWLNNVPIVPLNYVQFRLAVPLSEGTCKYTESGRSKIHSKLKLDLALL
                     LHMMRNSNYERSIEFVDNRISKYSAQKGKCAITGKFLEYEEIHCHHIVPVKQQGTDKY
                     SNLIIIHKNVHALVHAIEEKIIHKYLNVLNLTNEQIEKLNELRVKAGNSVLTV"
BASE COUNT         1023 a          421 c          507 g          697 t
ORIGIN      
        1 ttaggcactt aaaagaagaa aacataagtt tccttttaag gagaactgat attttgagca
       61 gtggaatgat ggggtaacgc cctgaaacgc tcccttaata ctccgactgg ctgtttaaca
      121 tatagctgtt agatggtcag aagctcggtg aagtcggctg aaagttaccg taaatatgat
      181 aaattcatat acgaaagttg ttcagaggtg aatgatgtaa cctataggtc ggaggtctat
      241 aaaatactca cggtgagaat gtaagtaata ctactgacga acttgcgaat gaaagaatct
      301 atacacggac tgtatagaaa tgtacaggat tctaccgtat caacggaaca gaatgtatgt
      361 tgtggtttag taagattatt ggttatgaac aaccatgcaa tgttacaggc attggaaata
      421 tacaggttca aagcaagcac ctaaaagtat atataaagat aaaaatatcg aaacatggga
      481 agctggcaat gtggaggaaa ctatattcca ttgaaacatt tgtgacgaag aataataagc
      541 acatttatgc cagtgaaagt ggtggcacag taccaatgag gatatagaaa tatatcggag
      601 gaatagccac tagtcaaaag aaatcgttaa ttaaatgatg tatttcatag aatcgagtaa
      661 gacaaaataa acactacaac ctaagtaaac aaaggtagtg gtgctatgaa taaagtaaaa
      721 acaaataaaa cacttcgtca tagtgaatac tatggcatga cacaaatatt tgatgaactg
      781 tatgtaaaaa gtgataaaaa agaagttttt acagagctca tgacaattat tatgagtgat
      841 gaaaatattc gactagcata cagaaatatc aagaataaca agggcagtta tacagcgagt
      901 accgataaga aaagtattcg agacattaaa acactgtcag aagatacctt tctaactgag
      961 gttaaagttc tttttcgctg ttacaaacca cgagcagtta gacgtgtaga aattcccaaa
     1021 ccaaatggaa aaacacgccc tctgggcatt ccatcaatct gggataggtt aatacaacag
     1081 tgtattttac aagtcttaga accgatatgc gaagctaaat ttcacaatta tagttatgga
     1141 tttcgaccaa accgttccac acatcatgcg attgctacct gcaatcgact ggcaaatagg
     1201 gggaagctac actttgtagt agatgttgat attaaaggat tctttgataa tgtcaatcac
     1261 tctaaacttt tgcgtcaaat gtggacgatt ggaatccgtg ataaatggct attaggtatt
     1321 attcgatcca tgctaaatgc tcctatagtc ctaccaaccg gtaaagtcga acaccctaaa
     1381 aaaggtgtac ctcaaggtgg tgtcctatcc cctttactat ctaatatcat attgaatgaa
     1441 ttagattggt gggttgcttc acaatggcaa aataccacga ttgagacaga aaagattatg
     1501 gataggcgag ataaaggcaa aggcattgat aagactaatc agtatcgggc attaagacga
     1561 acaaacttaa aagaaatata cattgttcgg tatgctgacg acttcaaaat cttttgtaga
     1621 aaacgaagtc atgcagataa aatatttcac gcaacgaaaa tgtggcttaa agaacggtta
     1681 tcacttgaaa ttagcgaaga aaaatccaaa attgtcaatc tcaaaaaagg taaatcagaa
     1741 tatctaggat ttgaattaaa gctaatcaag aaaggtaaaa aatacgtagt cgaaagtcat
     1801 atgagcaaaa aatcaatgac aagagttaaa atccaattaa agaaacaatt acgtaaagtt
     1861 gccagaccga agaatcattg cgaacaagca aaagaaattg gattatataa ttctatggtg
     1921 attggaatcc atcaatatta tggaattgca acctgtgtaa atctcgattg cagtaaaata
     1981 gcctatacac taaaaccgtt catcaatcac aaactgccga caaagaaaca tggaaagata
     2041 ttaaatacat ttatcaaatc gaagtatgga aaaagtaaag aaatgcgatg gttgaataat
     2101 gtgccaattg taccgctcaa ctatgttcag tttagacttg ctgtccccct atcagaggga
     2161 acttgcaagt acactgaaag tggacgaagc aagatacaca gtaaattaaa attagaccta
     2221 gcccttttac ttcacatgat gagaaatagt aattatgaaa gaagtattga atttgtggat
     2281 aacagaatat ctaaatattc tgctcaaaag ggaaaatgtg ctatcacggg taaatttctc
     2341 gaatacgaag aaatccattg ccaccatatc gtaccagtaa aacaacaggg tacagacaaa
     2401 tacagcaacc tcattattat tcacaaaaat gttcacgcat tagtccatgc tatcgaagaa
     2461 aaaatcatac acaaatacct gaatgtatta aatctaacca atgaacaaat agaaaaacta
     2521 aatgaattac gtgtcaaagc tggtaatagt gttttaacag tttaacttga aaaatatatc
     2581 atacttaaca ttcttttgat ggaacgccgt atgaagtgaa agtttcatgt acggtgtgaa
     2641 gcggggga
//
LOCUS       DACCDF010000167         2625 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCDF010000167 DACCDF010000000
VERSION     DACCDF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.176"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="IVK51_002784"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="IVK51_002784"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP6432316.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          943 a          458 c          581 g          643 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaa
//
LOCUS       DACCDF010000168         2618 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCDF010000168 DACCDF010000000
VERSION     DACCDF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.177"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(493..1785)
                     /locus_tag="IVK51_002785"
     CDS             complement(493..1785)
                     /locus_tag="IVK51_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432317.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(2059..2319)
                     /locus_tag="IVK51_002786"
     CDS             complement(2059..2319)
                     /locus_tag="IVK51_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432318.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          886 a          483 c          398 g          851 t
ORIGIN      
        1 ttatattata aattcacatg attatcgaat tttattaagt tagtttttct tgtaaatctc
       61 tgttcccaat gaaactcgag acacttattt taaagaggaa acaatgtggt atttaaatag
      121 ccctcttgta tgggctactc tgtgatactt attagccatt aaactatttt acccacatag
      181 catatatttt gaaatcgttc attccgtaaa ttcaactaaa tatgcctaaa aaaataaaaa
      241 atctaagata gaataagatt tattcagtct tagatggatt tatagcaact ttttcgtaac
      301 aactttaaaa aatacgaaaa gaaagagctg ttttactctg actacgttgc taaattgtcg
      361 tttaatcatt catttaaacc aaatcttttt ttatagaatt cgttttaatc ctcctgaatc
      421 caaaaactat gaaaaccgta attaaatagt taagagtttt taaccaccat aattctctcg
      481 gctattttga aatcagataa attttgatcg gacacatagt tgaataaacc taccgttgtt
      541 acggaaggta aatcgcatac ttttcattct agaggagaag gtctcgttga tcattaactg
      601 attaagcacc atagcccagt tttgaattcg accacctgac cacttcgcgt gtaattcttt
      661 cgtgcgtaaa taaagtagtt ttaggagtgc attctcatta gagaacgctc ctttttttgt
      721 gacttttctg aagctggagt ggacactttc aaccgcattg gtagtgtaca taatttttcg
      781 aatggcacta ccataatcaa atagttgttc aacatgtgca aagttccgtt tccagacatc
      841 tacagcacca gaataatgag accaccgatt ttgaaaactg tcaaaagcag catgtgcagc
      901 gtttagagaa gaagcaccgt agaacttttt catatctcgg cagacttcct tatagtcctt
      961 acttggaata tagcgcaatg catttcgaac aagatgaaca atacagcgct gaacaattac
     1021 cgatggaaag atcgcttttg cgccttcttc gaggccagaa acaccgtcca tcgaaatgaa
     1081 aaagacatct tcgacaccac gtgctttcag ttcatcaaat acttgcatcc agcgattttt
     1141 agattctgtt tgatttaacc ataatcctaa aatctcctta tttcctttga gatcatagcc
     1201 aagaatggtg tatacagcat attctttggc ttcataattt tctcgtaaag taacatacat
     1261 acaatcaacg aatagaaagg cataacactt tgctaggggg cgggcttgcc attcttccaa
     1321 ttcaggaagg acagcgtcag tgatatctga aatcatttca tgggaaatat caaagccata
     1381 aatagcttcg acggttgcgg caatatctcg ttgactcatt cctcgtgcat acatggaaag
     1441 aaccttccct tcgatgtcgg agacatctcg ttttctctta ggaattaact ctggttcaaa
     1501 ggaagcttcc cggtctctag gaacatcaat agctacttca ccaaaactgg ttttaagcgt
     1561 tttagttcca tagccatttc gacggttatc gtgttcctta ggctctttag aatgggcatc
     1621 ataacctaaa tgattattca attctccttg aagcattttt tcaaaaaggg gcccaaacac
     1681 atctttcaaa gcatcttgca tgtcatcgac agattcaggt tgataggcat tcagaatgga
     1741 ttcagctaac ttttcggcat caggatttct tttctttcta gccatcgtgt aatcacctct
     1801 tgatcttatt gtagaaaaag aaaaaccgcg aagcaaccac ctgcctagga ttaatggtta
     1861 cttacacggt ttatattaca ctctcatcat taaaaaagta tctttcgata ttactaaaca
     1921 atgaaattat ctatttgaaa tttaaatctg ataatatatt gagtgttcca atgagggctt
     1981 tgatcctatt atccatatct ttttcagttt taatattgat acacattata agaaaaagaa
     2041 acttttggtt aatttatttc atcccagtgc aaaatataaa aaagtaaaat agaccatagg
     2101 caatagatta acaattaata taatacaagc aaatattcta ttcattgtat ttctgctttc
     2161 aaaaagttct aatattgaaa gtataattcc acaaatgcct ataataagta tgggaataag
     2221 attagaaaat aacaccccga gccaatctgt gaaaattact atatttacta atatccctat
     2281 taacatacct atcatattta ttaaatcaat ttttttcatt agatatttct cctttattaa
     2341 atctgaagta cttatattaa aataattcta aactatttac ctcaaaaagt gcaataaatt
     2401 atttctgaac aataaaatga tagtcaaaac aaaaaaatat tgttaatcgt tcagagccat
     2461 ccgagagtgt aaaatatttt gtgtaaatga aaaaatccat acaaaaaagg aaggcgcttc
     2521 tgtagaataa agttaacgac aaccaattca cagaaaagag gacttcccta tgaatgattt
     2581 tactacagaa attgtgcaaa ctctagtcac taaaggcg
//
LOCUS       DACCDF010000169         2483 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCDF010000169 DACCDF010000000
VERSION     DACCDF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.178"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(1014..1304)
                     /locus_tag="IVK51_002787"
     CDS             complement(1014..1304)
                     /locus_tag="IVK51_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432319.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2203)
                     /locus_tag="IVK51_002788"
                     /pseudo
     CDS             complement(1512..2203)
                     /locus_tag="IVK51_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          734 a          458 c          348 g          943 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgatttct acatttgttt
     1681 tttcttgtgt cagtgttgcg atttgttgat tcaaaccatt tacagtattt tgtaattctg
     1741 aaagttttgc ttgtgcttct tgtagtcttg ctgtccaatc tgcattcaga tgattgattt
     1801 catcattttt ttggttgatc tcatctagtt tgacattgat ttcattttgt ttgctttgta
     1861 gttgtgcatc cttgctgact aattgacttt ctaagttttg tttttctgct gccgattgat
     1921 ttttttggtt ttctaattca tttacttttg tttggtattc ttggacatct gcctttgaat
     1981 cttgcaatgt ctgttctttt tgtgctagtc gagcggtaag atctgtaatc gcttgctctt
     2041 tatctgtcaa tgtatgatca agctgtgtca gattctcatt gattgtatca atatctgtct
     2101 ctccacccca caaaataatt tcatttgcgt accgcgtgcc tgtgtagcca attagacata
     2161 gaaatgcaac gacaatccca ccagtaataa atttcttttt catttgcata gctccttaat
     2221 ccaatacttt tacatgctaa aagtaagtga tctcaaacac aatgtcaagc gtttttaaat
     2281 tacatatgta attttaatat aaatactatc aaatactcat ttaatcctcc ttttttatct
     2341 atttaaaaaa tcttttttat agagaaaaat gcaggttaat actttcttaa ataaatattt
     2401 atggtgaaca aaaacaaaaa agcaagtttt attaaacgta acccttttca tttcatagat
     2461 tactgtattt attacaaaaa acg
//
LOCUS       DACCDF010000170         2397 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCDF010000170 DACCDF010000000
VERSION     DACCDF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.179"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            206..2068
                     /locus_tag="IVK51_002789"
     CDS             206..2068
                     /locus_tag="IVK51_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6432320.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="IVK51_002790"
     CDS             2068..>2397
                     /locus_tag="IVK51_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6432321.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DACCDF010000171         2368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCDF010000171 DACCDF010000000
VERSION     DACCDF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.180"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..65)
                     /locus_tag="IVK51_002791"
     CDS             complement(<1..65)
                     /locus_tag="IVK51_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP6432322.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYL"
     gene            complement(139..420)
                     /locus_tag="IVK51_002792"
                     /pseudo
     CDS             complement(139..420)
                     /locus_tag="IVK51_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            882..2264
                     /locus_tag="IVK51_002793"
                     /pseudo
     CDS             882..2264
                     /locus_tag="IVK51_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          861 a          402 c          396 g          709 t
ORIGIN      
        1 aggtagtaac caacagcgac aataatgacg tcttttttga attgtttgcc tttaaaatga
       61 ttcattactc tgtcctctct gtcttttttc tcaattttac actaaaatag attttttgga
      121 aaactttgca acagaaccga aatgagtata gtatgaatta gtaatttggt tttcatctaa
      181 gtttagcttt gatttactaa aattgatatc tcgatacatg ttcaatacat ctaatacaaa
      241 tcgagaatct tctttcggaa atggctcaga aagatcactc caaatttcat catcataatg
      301 atactcgtaa ccttctctca atatttcgat ttttctttca taataagatc tgttttggtc
      361 ctcaaattta gccaataact caaattgatt tactagtgac aatctctgaa aactgtccat
      421 taaatgtttc ttcctatctt gttcattaac aaaaaattat aagcccttac aacgaatatt
      481 atatattaaa tttaattgta ggtgaacacg aattttataa atatttatgg tgttaatgac
      541 aaatgatatc cgactcctct tcatacgttc tggactattt gctaatagat taaaatttca
      601 gcatctattt ctgaccataa ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa
      661 ataaaggatc aaaaactttt aagtttctga ccccgtaact cacacttcaa aatcttactt
      721 aaaaaggagt ctgaaagaaa aaaagagtat tcaaaatttt tttctaacta gctacatcac
      781 atctatgcat tgacactcaa agaaccagaa taagtcatca gaaagcaagg gggtgcggac
      841 ataatcctgg acgaaaaaat aaacttagga ggaatgtccg tgtgttttcc cacaaagagc
      901 gtgtaagggc aattcagtta tttttaaaat acgattgctc ttatgctgca accattcgag
      961 aattagggta tccgtcgatt ggtgcactac ggaaatggta taaagagtat ttggaatccg
     1021 gagagctcca tcaggagcac cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag
     1081 cggtgaatca ttattttgaa tatggtcaat gttatgcacg aaccattcgc atgcttggtt
     1141 atcctaatag agagtccttg cggcagcggt gtgaagaact tgcaccagga gctagaaaac
     1201 tccgaaaaag tgcggtaaag ttaacacaag atcaaaaaga agctgtgttg aaaaaattct
     1261 ataaaccgca aactaatagg aaaaatttag ccgaatctgg aggaattagt agagttactc
     1321 tatatcaatg gaaaaacacg gttcttggaa aggattttcc attgtgtatg actttaaaaa
     1381 atcaggaaaa acaaaaagag ctacttttaa aggaagtaga agacttacaa aaacaagtac
     1441 accaattaca gctcgaaaaa gcattactcg aaggagcggc tgaactgcta aaaaaagaaa
     1501 aaggcgtcaa tcttctttgt ctatcaaatc aagaaaaaac gatattgatt gatgccttac
     1561 gaaatcagtt caccttgaaa gagcttcttc aacagctcca gtttcctaaa agcagctatt
     1621 tttatcaaaa acaagcactt gagaaaccag ataagtatta caaagaacgt caaattaatt
     1681 atcacgattt tcaatcataa cttctgtgcg tatggttacc gtagaattca ccaagcttta
     1741 aaaaatatgg gtaaaaagct ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat
     1801 ctatttgtta aattttctag acgaaaaaag tattcttcgt atgctgggga aatttctcct
     1861 gcacacccta atttattaaa tcggaatttt aaagcaaaga agccaaatga aaaatggtta
     1921 acagatatta ctgaatttag aattctcgca gggaaaatct acttatcacc acttgtcgat
     1981 tgttacgatg gtgctattgt tagttggact attggaacaa agccaacttg cttcactcta
     2041 tgtctaaaaa gggttattca ccagataatt cagcgtgcga aggtttcttt ggacgattaa
     2101 aaaacgagat gttttatcaa cgtgactgga agaatacaac aatcaatcag tttataaaca
     2161 aattggatga ttatattcat tggtataata atcaacgaat taagttatct ttaggagggc
     2221 taagccctct tcaatatcgg aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa
     2281 tatccgcacc cccttttttt tgtaaaaaac tattgcacaa tacaagtagc ctacggctat
     2341 cgaaatttcc ataactttcg agcaagaa
//
LOCUS       DACCDF010000172         2349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCDF010000172 DACCDF010000000
VERSION     DACCDF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.181"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            13..251
                     /locus_tag="IVK51_002794"
                     /pseudo
     CDS             13..251
                     /locus_tag="IVK51_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            253..1551
                     /locus_tag="IVK51_002795"
     CDS             253..1551
                     /locus_tag="IVK51_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP6432323.1"
                     /translation="MKHTHKIILAVTSVCTVIILGVSIFFVTQAATNNKLENTSQTTQ
                     HSSSSSKPVEDKQTTKQLDQAKQLAASYHYDEAIALLEKDDAKEAQQLLATLKKEKES
                     LVKWEDPTKISHVFFHSLIVDPAKAFHTQQAQGYKDYMVTISEFNKTIDQLYKNNYVL
                     VNLNGLVKKGTDGKLTFTGVSLPEGKKPLILSQDDVSYYEYMDNSGFPSKLIVDKQNQ
                     IKNIYIDNKKETVGDYDMVPLIDSFIKKHPDFSYQGAKGTLALTGYNGVLGYRTSKSE
                     YGDNEKTHKEIEAAKKVADQLKEDGWSFASHTWGHLNMTQASLADIQQDNERWQNEVA
                     PILGKTNILIYPFGADISDWQPYSEANQKFAYLKQQGFDIFCNVDASTPAWGQLGTDY
                     YRNARINIDGIRFEADLKGENPILDHFINVKEMYDQKDRG"
     gene            2198..2349
                     /locus_tag="IVK51_002796"
                     /pseudo
     CDS             2198..2349
                     /locus_tag="IVK51_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          836 a          400 c          429 g          684 t
ORIGIN      
        1 ttttagtttt tcttggaaat aaaaacagcg aaaatccgaa cattttcacc tctctcactt
       61 tataactttt acaaaatagt acttctcata aaaagcgctc tagttaaaga aaataagaga
      121 aaaatcaaaa aaaacaaaag tttcttttac acttgtttct atattcgtta caatggttat
      181 gggaagtttt taaaaaggtt aagaaaagct tacgggggtt tttgaaagca aaaagtagta
      241 ggggacgata aaatgaaaca tacacacaaa atcatattgg ctgtcacttc tgtatgcact
      301 gttattattc taggtgtaag tatctttttt gttacacaag cagcaacaaa caataaatta
      361 gaaaacacta gtcaaacaac tcaacactca agcagcagct caaaacctgt tgaagataaa
      421 caaacgacga aacagcttga tcaagcaaaa cagcttgctg caagttatca ttatgatgaa
      481 gccatcgcct tacttgaaaa agacgacgcc aaagaagcgc aacaattgtt agcaactttg
      541 aaaaaagaaa aagaatcctt ggtaaaatgg gaagacccga ccaaaatctc acacgtcttc
      601 tttcatagtc tgatcgttga tccagctaag gcttttcata cccagcaagc acaagggtat
      661 aaagattata tggtcactat ttccgaattc aataaaacca ttgatcagtt atataaaaat
      721 aactatgttc ttgttaatct aaatggatta gtcaaaaaag gaacagatgg aaaactgacc
      781 tttacaggtg tttctcttcc agaaggaaag aaaccgttaa ttctttcgca agacgatgtc
      841 agctattatg aatacatgga caattcagga tttccaagta agttgattgt cgataaacag
      901 aaccaaatca agaatattta tatcgacaat aagaaggaaa ctgttggtga ctatgatatg
      961 gttccattga tcgattcatt cattaaaaaa catcccgatt tttcttatca aggcgccaaa
     1021 ggaacattag ctttaacagg ctataatggt gtattaggtt accgtacatc aaaatcagaa
     1081 tatggtgata acgaaaaaac acacaaagaa atcgaagcag ccaaaaaagt tgctgatcaa
     1141 ttaaaggaag atggttggag ttttgcttct catacttggg gacacttgaa tatgacacaa
     1201 gcttctttag ccgatatcca acaagataat gaacgttggc aaaatgaagt tgcacctatt
     1261 ttaggcaaaa caaatatttt gatctaccca tttggcgctg acatcagcga ttggcaacct
     1321 tattctgaag caaatcaaaa atttgcttac ttgaaacaac aaggatttga tatcttctgc
     1381 aatgtcgatg cctcaactcc tgcatgggga caactaggaa ctgactacta ccgtaacgcc
     1441 agaatcaata ttgacggtat ccgtttcgaa gctgacctaa aaggagaaaa tccaatactt
     1501 gatcacttta tcaacgtcaa agaaatgtat gaccaaaaag atcgaggata gaggttgtga
     1561 aagaagcgtc cagctccgag caaataagaa ctagatttct aaaatagttt ctcatatttt
     1621 ggaaatctag cgcttattgc tgaggagttg cttcttgaac accgtgaata gaggttgtaa
     1681 aaagagcgtt ttgacttatt gttgaaggtc agctctttga acaccgcgtg ttaggttgtg
     1741 aaaaggctgg tctgcatcaa gcaataagaa tgaacagaga aaaacagctt cttgaatacc
     1801 gcagataggt tgtaaaaaaa ccgttttgac ctgacgataa ccagtaaaac agaggtgaga
     1861 ccattgctaa aagtagcagt ggtctcatct ctttttttgc acaagtcata tttgattgtt
     1921 tcgttatttt aatcaggata aagttctttt tttcatgcta aagagtgatt ctttcttctc
     1981 gattttcact ttcttcgttt aacttttggt aaatacttct gtcagaaaaa gctctaggta
     2041 gacacacgaa agcagataaa aagcctaact tttgcaacag tttatgctgt cgttcaaagt
     2101 taggctttta cagtttataa atagggttct gttgcaaagt tttccaaaaa atctatttta
     2161 gtgtaaaatt gagaaaaaag acagagagga cagagtaatg aatcatttta aaggcaaaca
     2221 attcaaaaaa gacgtcatta ttgtcgctgt tggttactac ctgcgttaca atctaagcta
     2281 tcgttaagtt caggaattgt tatatgatcg tggaataaat gtttgtcata ctacgattta
     2341 tcgttgggt
//
LOCUS       DACCDF010000173         2230 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCDF010000173 DACCDF010000000
VERSION     DACCDF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.182"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(94..390)
                     /locus_tag="IVK51_002797"
     CDS             complement(94..390)
                     /locus_tag="IVK51_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP6432324.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IVK51_002798"
     CDS             746..1102
                     /locus_tag="IVK51_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP6432325.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IVK51_002799"
     CDS             1112..2011
                     /locus_tag="IVK51_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP6432326.1"
                     /translation="MGKLAIISDLHVDIKKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYEPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT          728 a          380 c          424 g          698 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaagaaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ctggaacaaa ttaaatgcat ttttaggatc agaatcattc ggagaattac ttgaccgtta
     1741 ttcaaatatc aaacaagtag tttttggcca tacgcatcgt cgctttgaag ataaatccat
     1801 cggtcacacg atttacagct gcagaccttt tggttattat tatgaatggc agctgacccg
     1861 agattttgtc ttgaaagaac agctgttaga tagctatgaa cccatgaaat tacgtgtcct
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat
//
LOCUS       DACCDF010000174         2189 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCDF010000174 DACCDF010000000
VERSION     DACCDF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.183"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(325..528)
                     /locus_tag="IVK51_002800"
     CDS             complement(325..528)
                     /locus_tag="IVK51_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432327.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="IVK51_002801"
     CDS             complement(987..1952)
                     /locus_tag="IVK51_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP6432328.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DACCDF010000175         2171 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCDF010000175 DACCDF010000000
VERSION     DACCDF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.184"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..174)
                     /locus_tag="IVK51_002802"
     CDS             complement(<1..174)
                     /locus_tag="IVK51_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP6432329.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFS"
     gene            complement(192..512)
                     /locus_tag="IVK51_002803"
     CDS             complement(192..512)
                     /locus_tag="IVK51_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432330.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            811..1587
                     /locus_tag="IVK51_002804"
     CDS             811..1587
                     /locus_tag="IVK51_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP6432331.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1602..1805
                     /locus_tag="IVK51_002805"
     CDS             1602..1805
                     /locus_tag="IVK51_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6432332.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1821..2159
                     /locus_tag="IVK51_002806"
     CDS             1821..2159
                     /locus_tag="IVK51_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP6432333.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT          818 a          346 c          369 g          638 t
ORIGIN      
        1 gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac cgtatagatt
       61 ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt tcatctccat
      121 ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaaac
      181 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
      241 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc tgataaatcc
      301 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gacatcgaaa
      361 tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca ctttgctaga
      421 taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt tttcctcttc
      481 gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct aaatgtttac
      541 aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa tgagctatac
      601 tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat tgatatgtaa
      661 atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta attatgctct
      721 tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg ttaaatttgt
      781 ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt taatttcgaa
      841 caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg caaagacgtt
      901 gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt agatttggaa
      961 gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac aatcatcaac
     1021 gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa aaaatttaaa
     1081 cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta tctaactcca
     1141 gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc aactcaacta
     1201 aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta cgaacctaaa
     1261 atctcctatt tagatagcat attatcttct acagattcag taacaattag tcagattgca
     1321 gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact aggtattcag
     1381 aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg atacacaaaa
     1441 tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat gaataccaaa
     1501 tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg atattaccct
     1561 caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac attgcagaaa
     1621 tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag ttcttaactt
     1681 acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta attttaaacg
     1741 gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact atgtacgaat
     1801 tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt ccagaaggct
     1861 atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat actggtagat
     1921 ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg tttacttcta
     1981 gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac aaaaatggat
     2041 ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt aaaacacttg
     2101 aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg aaaatttaat
     2161 agagcgctag t
//
LOCUS       DACCDF010000176         2123 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCDF010000176 DACCDF010000000
VERSION     DACCDF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.185"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..414
                     /locus_tag="IVK51_002807"
     CDS             <1..414
                     /locus_tag="IVK51_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432334.1"
                     /translation="EGVTGRKISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYE
                     ILPANQEYYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIH
                     HTEKHKIGVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            417..1166
                     /locus_tag="IVK51_002808"
     CDS             417..1166
                     /locus_tag="IVK51_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP6432335.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1178..1447
                     /locus_tag="IVK51_002809"
     CDS             1178..1447
                     /locus_tag="IVK51_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432336.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1609..1911
                     /locus_tag="IVK51_002810"
     CDS             1609..1911
                     /locus_tag="IVK51_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432337.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1908..2069
                     /locus_tag="IVK51_002811"
     CDS             1908..2069
                     /locus_tag="IVK51_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP6432338.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
BASE COUNT          824 a          348 c          473 g          478 t
ORIGIN      
        1 gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac tgtgagtgat
       61 gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat tcctttcaaa
      121 gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg catcacaaaa
      181 agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga caaagcgcta
      241 ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc actctgcaga
      301 atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa atatcaaatc
      361 aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg gtaaaaatga
      421 cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac aaaagactta
      481 aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa agaggctttt
      541 gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag accatatcgc
      601 gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt tacaaagagg
      661 gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt gacgaaaaac
      721 gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat aatacattta
      781 ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag gctaagcccg
      841 tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag atggagaaac
      901 tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca gagtattgtg
      961 aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg gcaaggaaag
     1021 aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga cgagagaagc
     1081 ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta gaacgagcta
     1141 atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt atctagaact
     1201 aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga gagacgtata
     1261 tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga gttggagtaa
     1321 gctcagatac tatttcgcac tttatatcga tacaacacaa ttaagaaatt tagcatatac
     1381 caaattacta tttgttgatt gtgttaaagg cttgcaaaaa catcttaatg aacttgagca
     1441 ggtgtaatca gatggaccta aagacattta cagcacagat tgaactaatg catcaagaag
     1501 ctttaagaca aagtgtatcg tacgaagaca agtggctcaa cacgttccat ggcggacgtg
     1561 agagcgcact tgatcaagta ctcaaattac tgaaaggaga atgtcgggat ggataagaaa
     1621 gcggcaatgc agcgaattat cgaattgact tattcagaag attggcaaaa tgacaaagaa
     1681 gctgcttcag aagtgatgag gcttggaaga gcgatgtggg cagacaagag caacaagcca
     1741 agaccacgaa aaatcgcaat ttggcacggt gacaaacttc tagtgatagg gacagctgaa
     1801 cagttagcaa gtctcacagg cttgcacgag aaaatcgtga ggaaaagagc aaggtgtggc
     1861 tacacagacg ctaagaagag aacgtttaga tacgtggagg aatcatcatg acaacagaag
     1921 aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg gaacgaacaa
     1981 aggcagtgat ggtcaatgaa acggcgagaa aggcaatcga tttgcacata gagaatttaa
     2041 gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa tgaaaagaat
     2101 tgctgaatta accaagtcag aat
//
LOCUS       DACCDF010000177         2095 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCDF010000177 DACCDF010000000
VERSION     DACCDF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.186"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            8..514
                     /locus_tag="IVK51_002812"
     CDS             8..514
                     /locus_tag="IVK51_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432339.1"
                     /translation="MDIKWLVQQNDSNLELAIKYLEETIFEDEHLTDNFLQVLKYLEI
                     YSVKKNKLIGENDSPIKTPIELSLRNRMGILQRSEIVKELFYHKFSYEIRLDDTYEHY
                     RIVFFVYNSIEDATATTALTFGFTKNGTINSDKTRQAATESDDICKKVCNGEENYWIG
                     EEKLNEIY"
     gene            501..953
                     /locus_tag="IVK51_002813"
     CDS             501..953
                     /locus_tag="IVK51_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432340.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            946..1344
                     /locus_tag="IVK51_002814"
     CDS             946..1344
                     /locus_tag="IVK51_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432341.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
     gene            1656..1729
                     /locus_tag="IVK51_002815"
     tRNA            1656..1729
                     /locus_tag="IVK51_002815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1690..1692,aa:Ile,seq:cat)
BASE COUNT          763 a          278 c          376 g          678 t
ORIGIN      
        1 tagtgagatg gacattaagt ggttggttca acaaaatgat agcaatttag aattggcgat
       61 taaatatctc gaagaaacta tctttgaaga tgaacatctt acagataatt ttttacaagt
      121 attaaaatat cttgaaattt actctgttaa aaaaaataaa ttaattggtg aaaatgattc
      181 tccaataaaa acacctattg agctaagctt aagaaatcgg atgggaatac tccaaagatc
      241 ggaaatagta aaagaacttt tttatcataa atttagttat gaaataagac tagatgatac
      301 ttatgagcat tatagaattg tttttttcgt atacaattct atagaagatg ctactgcgac
      361 tactgcatta acattcggtt ttacgaaaaa tggaacaatc aattcggata aaacccgtca
      421 agctgctaca gaaagtgatg atatatgtaa gaaagtatgc aatggggaag agaattactg
      481 gataggggag gaaaagttga atgaaatcta ttaatgattt attaaaaaaa catcctgatt
      541 tatataaaaa attaaattct gaacaaagaa acctgtccaa acaattactt aaaataagat
      601 taaaaaaaca aaaaaacgtg acagaattct caaaaatgtt aggaataagt gaaaaaaaat
      661 atcttgaatt tgagttcgga gatatgcata ttcctgttgc agagtacaat aaacttttga
      721 aacaagttga aaatttgtcc tataaagatt caatgaattt cactattaag gttgctggcg
      781 gccctattaa atatgaccgt attgaaaata aaccttatat tcggaataaa agttactcag
      841 tatttaaaag tagaaagaga aaagaagtat cgtcaaatat ggtctatgct agtgttaatt
      901 accaagatac gagccgctat attgtagaaa aagaggtata tgtacatgga taaaggaaat
      961 agtgttactt ttgcacttga aaatattagt ttgaataaaa ttcattttga attaactgat
     1021 aattttagtt atggagattt agagtttaat attgaacttg gatataaaga agacgaagca
     1081 gagtatctag aaaaaaaact tgtttcttta cgagctcaca ttgtaggggg gatggaagga
     1141 gcaccggtag atattttact cttttcagtt attcgtgttt ctacaaatga acagccatat
     1201 aaaaaattag tagaagaatc tatgggagag ctagctaaac ctatcctgaa taaagcttct
     1261 aatttgattt ctaatttata tagcgaagta ggagttatgc ctacagttat tgatttatat
     1321 cagctatata gtcaacaaaa ataaaataca agtaatagag ttatctattt aaatttgcaa
     1381 gacaaagacc tcaaccagtc atccttggtt gaggcttttt tgctctgtaa ttaactcgta
     1441 tagatcctgc aagatagttt gtttctgaat aaatgattga tatgaagtat ttagatgact
     1501 gtattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata tcttcactat
     1561 cacccataat agtcacactc caagctatgc gataacagga ttgttgccac accttctact
     1621 ggttgattgt ttatggcttt atgtggcaac aacctgtact cttagctcag ttggtcagag
     1681 cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat aatttaatct
     1741 atttgataaa aaataaaatt caataatggt ataataaaat gtcggctcgt cgcttccaca
     1801 gagattttcg tgggaggagg tgtatgattt gtttgaattc actttggagc acgtgctctt
     1861 gccattgtta attggtttaa ctttggcatt atttagtcat tggctagata aacattagag
     1921 ccgacaatgg cgcttactgt ttcgcagcgg taagcgtctt tttttgaaaa agaaaaaaac
     1981 acaacgtatt cgcaggtacg ttgtgtttat ggtgtatatt tgtttaaatt cactttcaat
     2041 tattattata cttgttaaat aaattgtgtc aatataaaag atatttttat acgtg
//
LOCUS       DACCDF010000178         2051 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCDF010000178 DACCDF010000000
VERSION     DACCDF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.187"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..612
                     /locus_tag="IVK51_002816"
     CDS             <1..612
                     /locus_tag="IVK51_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP6432342.1"
                     /translation="IGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINL
                     AREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV
                     RRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            complement(742..>2051)
                     /locus_tag="IVK51_002817"
     CDS             complement(742..>2051)
                     /locus_tag="IVK51_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP6432343.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          694 a          359 c          222 g          776 t
ORIGIN      
        1 attggaataa ccaaagatgg cgaccgtgaa attatcggct tcatgattca aagtggcgaa
       61 agcgaagaga cctggacaac attttttgaa tacctaaaag aacgcggttt acaaggtacg
      121 gaactcgtta tttctgatgc gcacaaagga ttagtctctg ccattagaaa atccttcacc
      181 aacgtaagtt ggcaaagatg ccaagttcac ttcctaagaa atatctttac caccattcct
      241 aaaaaaaatt caaaatcttt cagagaagct gttaaaggaa tttttaagtt cacagatatt
      301 aacttagcgc gtgaggctaa aaatcgattg attcatgatt atatcgatca accaaaatat
      361 tcaaaagctt gcgcatcatt ggatgatgga ttcgaagacg cctttcaata taccgtacaa
      421 ggaaattccc acaatcgact aaagagtacc aatctaattg aacgactgaa tcaagaagta
      481 cgcagaagag aaaagattat tcgcatcttc cccaatcaaa catcagccaa tcgcttaatt
      541 ggagccgttc ttatggacct acatgatgaa tggatttatt cttcaagaaa atacatcaat
      601 tttgataagt agaaatggta aaaacattgt atagcatttt acacaggagt ctggacttga
      661 ctcacttcct ttattatttt tcattttttt aaactattta atactaatgt cttttataat
      721 agcttttcat atattatata atcaatcttt ataagtcctt ttataaattt cttttctacc
      781 attttcgata aattcctgtt taatattttt aattccataa acaatagttt caataggata
      841 atattcttca actatatctt gatattcttt tgctttctca atatctatat ttccatacat
      901 tcttaatata tcttctccaa aatttgttcc tatttcttct tcactatctt caagtaagta
      961 tataaaatca caatattcat ctataattcc agaatctcca aaatcaatta ttccagttaa
     1021 tctattattg ccatctaaca atagatgatt acaactaaaa tcattatggc ataaacactt
     1081 tttaccctca aaaactgttg ttgcatttag tctttccata aaactttcta tataatcttt
     1141 ttctatatca gttaaatcat tataaatagt ttcacgcaac aatatatact cttctaatac
     1201 attttgttta ttatcaatag tacattcact aatatctgta taatctaaac cgtgcatttg
     1261 tcttaaaaaa ctggcaatat ctcgttttaa caaattttgt tcttcttctg acatagtaga
     1321 ataaatttct ggtgttaaaa aagttccttt aatttcttta taacctagta tagataattc
     1381 atcactaata tacgaatatt caatattagg aatttttaca ttagtttcta aatttgtatt
     1441 taaaaaatta tatattgctt tttcttttgc ataacctttt ttcttattag tactaaattt
     1501 tgttttaaaa atgtattcat tattaactaa atatgccaca ctatcataac cactaccgat
     1561 tatttcaata ctatctactt tgaaattatc aaagtaatgc tcaattaaat atttcattgc
     1621 cttaacattt gtggcattat catcatatct atattccatt aaataacaat cttctttttt
     1681 gccctcgtgt aattcatgtt ctggcaaatc ttcaataatt ctaaaaccag atttttggta
     1741 tgcccttatt gctcttggat tatttttatg agggtctaaa ataactgcat tagcatttct
     1801 ttcttttttc aaaaattcaa aaatcaattt aatatatctt gtaccaattc ctttactcca
     1861 ataatttggc tctcctataa attgatccat accatagact atctcatcag tttttggata
     1921 atgataatca gtatataact catcatacat tttatatatt tgtccatatc caataggaac
     1981 attgttatat tcaataatta ctctaaaaac ttcatcttcc caaggctctg tataatgttt
     2041 ttttaatgat t
//
LOCUS       DACCDF010000179         1890 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCDF010000179 DACCDF010000000
VERSION     DACCDF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.188"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            715..1800
                     /gene="xerS"
                     /locus_tag="IVK51_002818"
     CDS             715..1800
                     /gene="xerS"
                     /locus_tag="IVK51_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP6432344.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT          704 a          317 c          257 g          612 t
ORIGIN      
        1 aaaaccacta gcccgccagc tagtggtttt tggtgtatca tgaattacct tttcgttaaa
       61 acaattatac catatttcct aaaaaataca attaatagac gaattatcat taccactttg
      121 atgaaaaaag ctgacataaa tttttctttt atttttataa tgaataaaca tttatcaata
      181 agcttttgaa aggcaggatc gtactttgtt gatgattgag atattgctac atcaatctca
      241 gcagttaaga tttttttgaa ttgttgcttg aattttttct ttatctaaaa aaattgtatc
      301 tccaggatgc aacccatttt ctttaagaaa ttccttttaa attgttaata ctactgagtg
      361 ccccacttct ctgaattttc tttaacttct ccctcccctt ttttatttgt atatacattc
      421 atatacgctt gttaatatca aatcaaccgt agtctctctt atgtctaatt atatagaaag
      481 attgttcttt ctatataatt tagacaattt aaaaaattaa gattgcctcc ttctgtttcc
      541 tttcctattc atgtatacca tttattttaa attattaaat ctatacctat ttatctgctt
      601 ttttgaaata aattaataaa tttcagagta taccaattga caaaacactc tataattggt
      661 atagtccaat tatagagata ctcctctccc ctacttatcg aaaggatgaa taacatgaaa
      721 aatacagaaa tatatcaaaa aatagataca atcatacttc atatgcccga ttatataaaa
      781 gattttgttc aagatcgtga ggataaagat caatcacctc gaactacttt agaatatcta
      841 aaaaattata agctctttta tgaatggctt ctgagtgagt ctatcgttcc cgatacttca
      901 atcacctcga taagacagtt aactgcctat gatctaaact tgtacaaatc tcatttgaaa
      961 cgtcgcacaa aggaaaatgt aaaaaaagag acaacaaaaa ctaagctcaa agataataat
     1021 aacttaggtc tatctacttc tactatcaat agaaatatca cagcattaaa ggttctattt
     1081 aaatatcttt cgaaaagttc caataatcct ttaggaaaac catacttaga aaataatcct
     1141 atggatcaag tagcaactat cacagataaa actacgttag ctgcccgtgc aaacgcaatc
     1201 gagaaaaaat tatttctaga cgaagataca caaaattatc tagattatat tgccaatgaa
     1261 tataaaaaaa ctttgagcaa acgagctttg atctattatc aacgagatgt agaaagagac
     1321 ctggcgatca atgcattgat tttaggatct ggattaagac tctccgaggt tgtaaatatt
     1381 aatttagagg acctatcttt agaaaagaat aatgtggtag taactagaaa aggaaataag
     1441 agagacgcag taaatatagc agcatttgca atggaatatt tagctaatta tctagctatt
     1501 cggaaagaac gatacaaagt aacagaaaac gaaaaagctt tatttctagc aatttatcaa
     1561 ggagaagcca agcgtatatc cggtattgca atcgaacgga tggtagcaaa atattcaaaa
     1621 ggattcagag tccaagtcag tccacataaa ttacgtcata ctttggctac tagactctat
     1681 caacagacaa attcattggt attaactgcc caacaattgg gacatacaag tacaaataca
     1741 acaactttat atactcatat agacaatgca gctacaattg atgctttgaa cgctttgtaa
     1801 atgtagtaaa aaatagtata ataatgaagc ctgtctctaa aattagataa aagcccaact
     1861 ttaaaggcaa gcttcattat catttttata
//
LOCUS       DACCDF010000180         1701 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCDF010000180 DACCDF010000000
VERSION     DACCDF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.189"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..255
                     /locus_tag="IVK51_002819"
     CDS             <1..255
                     /locus_tag="IVK51_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432345.1"
                     /translation="NFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSVI
                     SEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD"
     gene            258..411
                     /locus_tag="IVK51_002820"
                     /pseudo
     CDS             258..411
                     /locus_tag="IVK51_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            434..1639
                     /locus_tag="IVK51_002821"
     CDS             434..1639
                     /locus_tag="IVK51_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP6432346.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT          547 a          322 c          377 g          455 t
ORIGIN      
        1 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
       61 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
      121 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
      181 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
      241 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaacccct
      301 cggtatctaa caggggggta caaatcgaca ggaaacagtc aaaaaaacat tagaaaatcc
      361 tttggttaca agggatttac aaaatttcag cgtatgtcaa atgggcttta aaagttgaca
      421 tacgcctttt tgattggagg gatttttact gaatgaacaa acttggttac agcatttaaa
      481 agaaaaacgc ttggcttatg gactatctca aaaccgttta gctgttgcga ctggtattac
      541 aaggcagtat ctaagcgata ttgaaacagg aaaagtcaag ccatcagagg atttacagca
      601 gtccctttgg gaagctctgg aacgcttcaa tcccgacgct ccccttgaaa tgctgtttga
      661 ttatgtaaga attcgctttc cgacaacaga cgtacagcag gtggtcgaaa acatcttaca
      721 actgaaactg tcctattttc ttcatgagga ctatggtttc tattcttatt cagagcatta
      781 tgctttaggc gacatattcg tcctttgctc ccatgaactg gacaaaggag ttctggtgga
      841 attgaaaggt cgtgggtgca gacaatttga aagctatctt ctggcacaac aaagaagctg
      901 gtatgagttc tttatggacg ttttggtggc tggcggtgtg atgaaacgcc ttgaccttgc
      961 cattaacgat aagacaggga ttttaaatat ccctgtactc actgaaaagt gccaacagga
     1021 agaatgtatc tccgtcttcc gcagttttaa aagctatcgc agtggcgaac tggtacgcaa
     1081 agaggaaaag gaatgtatgg gaaacaccct ctatatcggt tcattacaaa gtgaagttta
     1141 tttctgtatc tatgaaaagg actacgagca gtacaagaaa aatgatattc ccattgaaga
     1201 cgcagaagta aaaaaccgtt ttgagattcg attgaaaaat gagcgtgcct attatgcagt
     1261 ccgtgattta ctcgtctatg acaatccaga gcataccgcc tttaaaatta tcaatcggta
     1321 tatccgtttt gtagataaag acgattccaa acctcgttct gattggaaac tgaatgaaga
     1381 atgggcttgg tttattggga acaatcgtga acgattaaaa ctaaccacaa aaccagagcc
     1441 ttactccttc caaaggacgc tgaactggct atctcatcaa gttgccccga ccttaaaggt
     1501 tgcgattaaa cttgatgaaa tcaaccagac gcaggttgta aaagacattc tcgaccatgc
     1561 gaaactgaca gaccgacaca agcagatttt gaagcaacag tcagtaaaag aacaggacgt
     1621 gataacaaca aaaaaataac tcaaatacaa attcattgaa tatagagagg agaacatttt
     1681 tatgaatttt ggacaaaacc t
//
LOCUS       DACCDF010000181         1679 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCDF010000181 DACCDF010000000
VERSION     DACCDF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.190"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(53..124)
                     /locus_tag="IVK51_002822"
     tRNA            complement(53..124)
                     /locus_tag="IVK51_002822"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(89..91),aa:Glu,seq:ttc)
     gene            complement(136..209)
                     /locus_tag="IVK51_002823"
     tRNA            complement(136..209)
                     /locus_tag="IVK51_002823"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Ile,seq:gat)
     gene            complement(222..292)
                     /locus_tag="IVK51_002824"
     tRNA            complement(222..292)
                     /locus_tag="IVK51_002824"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Gly,seq:tcc)
     gene            complement(297..369)
                     /locus_tag="IVK51_002825"
     tRNA            complement(297..369)
                     /locus_tag="IVK51_002825"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Phe,seq:gaa)
     gene            complement(373..445)
                     /locus_tag="IVK51_002826"
     tRNA            complement(373..445)
                     /locus_tag="IVK51_002826"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Asp,seq:gtc)
     gene            complement(464..537)
                     /locus_tag="IVK51_002827"
     tRNA            complement(464..537)
                     /locus_tag="IVK51_002827"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Met,seq:cat)
     gene            complement(550..639)
                     /locus_tag="IVK51_002828"
     tRNA            complement(550..639)
                     /locus_tag="IVK51_002828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(601..603),aa:Ser,seq:tga)
     gene            complement(662..733)
                     /locus_tag="IVK51_002829"
     tRNA            complement(662..733)
                     /locus_tag="IVK51_002829"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(698..700),aa:Glu,seq:ttc)
     gene            complement(755..842)
                     /locus_tag="IVK51_002830"
     tRNA            complement(755..842)
                     /locus_tag="IVK51_002830"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Ser,seq:gct)
     gene            complement(867..940)
                     /locus_tag="IVK51_002831"
     tRNA            complement(867..940)
                     /locus_tag="IVK51_002831"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Ile,seq:gat)
     gene            complement(954..1024)
                     /locus_tag="IVK51_002832"
     tRNA            complement(954..1024)
                     /locus_tag="IVK51_002832"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(990..992),aa:Gly,seq:tcc)
     gene            complement(1026..1101)
                     /locus_tag="IVK51_002833"
     tRNA            complement(1026..1101)
                     /locus_tag="IVK51_002833"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1066..1068),aa:Asp,seq:gtc)
     gene            complement(1120..1193)
                     /locus_tag="IVK51_002834"
     tRNA            complement(1120..1193)
                     /locus_tag="IVK51_002834"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Met,seq:cat)
     gene            complement(1206..1295)
                     /locus_tag="IVK51_002835"
     tRNA            complement(1206..1295)
                     /locus_tag="IVK51_002835"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1257..1259),aa:Ser,seq:tga)
     gene            complement(1319..1392)
                     /locus_tag="IVK51_002836"
     tRNA            complement(1319..1392)
                     /locus_tag="IVK51_002836"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1356..1358),aa:Ile,seq:cat)
     gene            complement(1406..1479)
                     /locus_tag="IVK51_002837"
     tRNA            complement(1406..1479)
                     /locus_tag="IVK51_002837"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1443..1445),aa:Met,seq:cat)
     gene            complement(1507..1579)
                     /locus_tag="IVK51_002838"
     tRNA            complement(1507..1579)
                     /locus_tag="IVK51_002838"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1544..1546),aa:Ala,seq:tgc)
     gene            complement(1599..1672)
                     /locus_tag="IVK51_002839"
     tRNA            complement(1599..1672)
                     /locus_tag="IVK51_002839"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1636..1638),aa:Pro,seq:tgg)
BASE COUNT          465 a          457 c          394 g          363 t
ORIGIN      
        1 taaaatcatt ctttcagata aaaagaaaaa gatactcaaa aagtatcttc catgacccgt
       61 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
      121 ggccaataaa ataaatgggc ctaaatggac tcgaaccatc gacctcacgc ttatcaggcg
      181 tgcgctctaa ccagctgagc tataggccca taaaaactaa aagcgggtga cgagaatcga
      241 actcgcgaca acagcttgga aggctgtggt tttaccacta aactacaccc gcataatggc
      301 gcgggacaga atcgaactgc cgacacatgg agcttcaatc cattgctcta ccaactgagc
      361 taccgagcca aacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc
      421 atgttaaccc ctacaccacg gaaccaatgt ttttagtttt taattgcggg agcaggattt
      481 gaacctacga ccttcgggtt atgagcccga cgagctacca gactgctcca tcccgcgata
      541 atatgaaaaa ggaggataag ggattcgaac ccttgcacgg ttttacccgc ctgacggttt
      601 tcaagaccgt tcccttcagc cggacttggg taatcctcca tgtaaaacta tattaaatta
      661 atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct
      721 tgaccaacgg gccaggtaaa acaaaatatt gaaacggaga aggagggatt tgaaccctcg
      781 cgccggtttc ccgacctaca cccttagcag gggcgcctct tcagccactt gagtacttcc
      841 ccaaaaccaa taagattttg tactaatggg cctaaatgga ctcgaaccat cgacctcacg
      901 cttatcaggc gtgcgctcta accagctgag ctataggccc ataaaaaact aaaagcgggt
      961 gacgagaatc gaactcgcga caacagcttg gaaggctgtg gttttaccac taaactacac
     1021 ccgcatggcg gttccgacgg gaatcgaacc cgcgatctcc tgcgtgacag gcaggcatgt
     1081 taacccctac accacggaac cagtgttttt agtttttaat tgcgggagca ggatttgaac
     1141 ctacgacctt cgggttatga gcccgacgag ctaccagact gctccatccc gcgataatat
     1201 gaaaaaggag gataagggat tcgaaccctt gcacggtttt acccgcctga cggttttcaa
     1261 gaccgttccc ttcagccgga cttgggtaat cctccatatg aaacttacaa cgccacaatg
     1321 gaccttgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc taaccaactg
     1381 agctaaaggt ccaggctctt aaaaatcgcg gcggagggga tcgaaccccc gacctcccgg
     1441 gtatgaaccg gacgctctag ccagctgagc tacaccgcga aaaagatatt cattatttaa
     1501 atgaaatgga gcctagcggg atcgaaccgc tgacctcctg cgtgcaaagc aggcgctctc
     1561 ccagctgagc taaggccccg tgtttaaatt tataattatc gggaagacag gattcgaacc
     1621 tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc cgtttaatt
//
LOCUS       DACCDF010000182         1637 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCDF010000182 DACCDF010000000
VERSION     DACCDF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.192"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(183..1502)
                     /locus_tag="IVK51_002840"
     CDS             complement(183..1502)
                     /locus_tag="IVK51_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP6432347.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDKRAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
BASE COUNT          485 a          300 c          325 g          527 t
ORIGIN      
        1 attctttggc acattttatt gaaatatgta tcactaatat ccctatcaac tgattttgac
       61 taaaaagtta cttcaaacat tcactgttca aaaatattga ggaataaatt ttgggtgtaa
      121 ggcattttga gcatacttct ccctggataa cttcccagta aaaaaatcgt tagattgttg
      181 gattagatcc attgactggc tcgcctcagg cgagcaaaga ccctgtagaa ttcattctga
      241 tacacatgat aactagataa tttgagatag actcgtctac ccgtttggac taatttccca
      301 gcaactttca gaaacttcaa tcgaatagaa tgaatggtca ttcccttttg gacttcttca
      361 aagccaatcg ttcttaagaa gttgactaag ttgtaagcta tcaagctgag catcattcgg
      421 acatgattct ccaaaaagcg aggactgtct gttttgtcaa agtaaaagcc agctttcgct
      481 tctttaatga agttctccat tgtgccacgt ttggcataga tagaaaagat ggtatcagga
      541 gaaacatttt ctgatagatt cgtcacgata aattcatgtc gaaagagtag ttcgcccgct
      601 tcacgtgttg agcgtataca cacgcgacga ctttgtgacc aggattgtgc ttgataagtg
      661 gtcgagaagt actgaacttc tcgttcttcc cacttttgat tatcgccata aagtactgat
      721 ttctcagcta tttgacctag tctacgatta ttcttcagtc gaataacata atgactcttt
      781 tttgattcac acgaatcata cacaccaggt gtagcgaacc cgctgtctcc acgaaccaag
      841 atgtcagtgt ttggtagaga gtgattatag tgctctaata aaggtgtgag aaactccttt
      901 acgccttttg atgtatattg atttcctgaa cgtagttcag cttttaagaa gtcaccagtc
      961 aatccgtcaa aagctaccaa tggatggtaa ccataggttt ggtagtgtgt attataattc
     1021 gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat ctaaatcaat gattaattcc
     1081 gtatcattac ggatgaggcg tgctttatcg ataagttctt ggttcaaagc ttgaagttca
     1141 tggatattct cctcagatag tcgatctaaa aatcgggaaa gtgaagattg agaagcgagt
     1201 tctttcctat ctaaaacagc tttaaacaca ggatcttgtc tcaggagatt agctgcagaa
     1261 tcagctgagt aaccagcaat taattgcata atgaactgct caagtatgga taagttgtca
     1321 tgagtaaaat acgctcgttt gtcttcaatg tgaatgtgtt gcttagccag ttcagaaaaa
     1381 ccaaagatgt tcatcagctc tttcactagg acgagacccg aatcactcga taattgacca
     1441 cctgtatgag aaatagtgat atttgaattg aattttactt ggtttttgtg taagctagtc
     1501 atgaaaagaa ctcctttctt ttggttggtt tagtcacttt aaccatagca gaaaggggtt
     1561 cttttttcat cacttaacgg gtgaagatga aaaagtaatt gtaagctgcc gtgaggcagt
     1621 ttcacatggt tttaatt
//
LOCUS       DACCDF010000183         1583 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCDF010000183 DACCDF010000000
VERSION     DACCDF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.193"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            291..1478
                     /locus_tag="IVK51_002841"
     CDS             291..1478
                     /locus_tag="IVK51_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6432348.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          316 g          504 t
ORIGIN      
        1 tttaaccttc cggttttgat tggataaccc tatgaaccat cgttatttta atttggtatt
       61 aactcatttc ttttgagata atatatttat tggattgaga ggctaagccg ttcccccctt
      121 gggccattga acccgcactc gagagactgg ctcctctctc tgataagtgg attgcgtatg
      181 agttagatgt tgaaagtcct tactttactc cgtatctcgt acaaggtagt gacgcttttc
      241 agttgagtgg atttcaatcc aaaattcttt ttttgaaagg tcagatatac atgtactttt
      301 taggcattga tatcggtaaa cgcactcacg ttgcttctat catgaacgag gaaggaaagg
      361 tattactaaa aggtttttca tttcctaaca ccactgaagg agcagagtca ttaattgagc
      421 gaatggtcga ctattccgga gcacccagtg actttgttat agggatggaa gccacggggc
      481 actattggct gtccatcttt tcataccttc acgaatcaga ttatttgatt cacgttgtga
      541 atccccttca aacagacgga tggcgtaaag gtacagaaat acggaaacga aaaaacgata
      601 ttattgattc tgttttgatt gctgacttaa tgcgctatgg ctcatttgtc gaaacggtct
      661 tatccgatga aaatgttttt tctttgaaac aactttctcg ttaccgaact tatttagtag
      721 gtacagcgag cgacttcaaa agaaaaatta tagccgtatt ggatcaagtc tttccagaat
      781 acgcgacgat ttttaccaag cagggcgttt tcggaaaagc ttctaaagag ttattgctgg
      841 cgtttagtgc cccgatcgac ttagaaaaca tttcctcaga aactttggcc caatacttag
      901 ctgagttaag caggaaccga atcaaggcag aaaaagcgga ccaattgaag caagcagctt
      961 ctaactcctt tggagtaaaa tttgctcaag aagcgttcac cttccaacta cgttcaatga
     1021 ttgaacaatt aaaattcatt gaaagccaaa taaaggaaac ggaaagagaa atcaagcaca
     1081 taatggacac tttaaattct gttattctca caattcctgg aattggtcct ataaacggtg
     1141 cgactattct tggtgagatc ggagatattc aaaaattttc taacccgaaa aaactcgttg
     1201 cttacgctgg gatagacgct tccgtgacac aatcagggca atatgaggca acgcataatg
     1261 ttatgagtaa acgagggtct ccatatttgc gaaaagcctt gttttcggcg gctttagtag
     1321 cttctcaatg tgatcctgtt tttagtgcgt tttaccagaa aaaagtttcc gaaggaaaac
     1381 atcatctaac tgccttaggt gcggtttctc gtaagctttg ttacgtgatt catgctattt
     1441 tgactaaaaa cgagccttat ataattgttt ctgattagct attgaacatt caaaaaaact
     1501 aatggtaaga agcttatttg ttgtaccctt ttttcggaat tatctgttat aaaatatttt
     1561 ttgatttggc taatttttac ttg
//
LOCUS       DACCDF010000184         1581 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCDF010000184 DACCDF010000000
VERSION     DACCDF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.194"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            128..1297
                     /locus_tag="IVK51_002842"
     CDS             128..1297
                     /locus_tag="IVK51_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432349.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          568 a          254 c          258 g          501 t
ORIGIN      
        1 atatcccata ttttaaaata aaagatagcg taaaatattt tgtgtaaata gaaatttagg
       61 gtagaaaaaa aagtccattc tgtaaaatta aagttaccac acagaaattt tataggagga
      121 ctttctcatg actaaattta ctatagaaat tatgcaaaca ctactcaata aaggggattt
      181 agatgaatta tttcaggatc atttagagcg agcgattaat tcacttctac aagcagagct
      241 aactgctttt ttagattacg aaaaatatga tcgaaatgga ttcaattcag gaaattcccg
      301 taacgggaat tattcacgta catttaaaac agaatatggc gagttgaatt taactattcc
      361 tagagatcgg aatggcgaat tttctcaaca aacactacca gcatataagc gaaccaatga
      421 ttcattagaa acgaccatta tccaattatt tcaaaaaggc attactatgg ctgaaatctc
      481 caaattaatt gaaaaaatgt atggtcacca ctatacgcca caaacaatct ccaacatgag
      541 taaattggtg gctgaagatg ttttagcttt taaagaaaga acgttagaag ccaattattc
      601 cgttatattt atggatgcga cgcatatccc tgtgaagcga cagactgttt cgaaggaagc
      661 agtgtatatt acgattggta ttcgtttgga tggaaccaaa gaagttcttg ggtttactat
      721 tgccccaact gagtctgctt atatttggaa agaagttctt caagatctta gaaaacgtgg
      781 gttagaagaa gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca
      841 tagtgtgtat ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc
      901 tcataaagtt cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca
      961 agcgaattca aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa
     1021 gcagtatcca agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt
     1081 ccctcacgcc atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca
     1141 gctaaaacga tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt
     1201 cttcatttct caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt
     1261 taaagaaatt caggatacat tagagtcgat gatttaactg taattaacag aatggatttt
     1321 ccatttacat ataattcttg acgctaccat aatctaaata accatcatat tcatctacta
     1381 cttatataat tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa
     1441 tctctgcatc cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt
     1501 tcaaatcctg atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt
     1561 tacttccatg gataataata a
//
LOCUS       DACCDF010000185         1516 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCDF010000185 DACCDF010000000
VERSION     DACCDF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.195"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(159..1178)
                     /locus_tag="IVK51_002843"
     CDS             complement(159..1178)
                     /locus_tag="IVK51_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432350.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1189..1395)
                     /locus_tag="IVK51_002844"
     CDS             complement(1189..1395)
                     /locus_tag="IVK51_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6432351.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          421 a          315 c          260 g          520 t
ORIGIN      
        1 caaccagtag aatgtgtggc aacaaacctg ttatcgcata gcttggagtg tgactattat
       61 gggtgatagt gaagatatgc gataacatca ctattttatc gaatgatttt tatagttgtc
      121 aatatagtta tgtaatgctc ctcaacgagg agctattttt atcgtttagg aatatttaaa
      181 taccaacgtt tgtcatggaa atcttgcgca ccgcctttag tgtttccctc tggatcattc
      241 gttgcccgca tcatgacata gactttctta ttagggaagt tacgcatgtt aaaagataca
      301 tgatagccaa catttccaga agtattataa gcttgattta catctgatct ataaattcca
      361 tcagctctta ctcgagctaa ttctttccca gtattgtaat ccataatgaa gatatactcg
      421 tatttatagt tagcaatgtg ccatccagct acatgcaaat ttgcattttc gatttctcca
      481 aactgatcaa tgtgggcgta attcgttcca tctgtcagtg taggattagc tgcaccagct
      541 cgtgttggat caatgacagg cttgttttca gaagttgttg gattttcatc ggtaaatcca
      601 tgagctaaat catatgctaa tttttcttta cttacgccca tttcagaaag ataaccgtaa
      661 ggatctgtat gatcgcccca gatgttttgt gttacccata aatgcgattt gattcccggt
      721 tggttataag gagtgtccaa cgttaatgga ataccatatt tcattgctga atctctagcc
      781 aattcaacgt atgccttgta gtttttctca aacgttgctt tatcatgtgt gtgttgtaac
      841 tcaatctgca caggactgtt ggcattagca tacgaaccag caccgtactg tacataacca
      901 ggttgaccga cttgataaac aattccgccg tctcccacaa tataagcagt ataagcgcta
      961 gtccatgaac gttgcatata ctgcgcttca ttgcgtcctg ttgctgtttc attagccgtt
     1021 tcatgcagta aaatatactg attatttgct acttgtgagc taccttcatt tgcgcccaaa
     1081 ttaaattcat tgttgatagt ataggcaaat ccgttaattg gcaataaaaa aagagccgtt
     1141 aacaggctca tcgcagtaat agtaattttc tttttcatat ctatcctcct atttatctga
     1201 tgaaattttc ttatacgttc gatttgatac acctaaaata ctccctagaa atgctccaaa
     1261 acctgtaata atcatcaccg ttatatctgt gtaccgccag ttaacacctt ttccgacaag
     1321 accgacaaaa gtcgctaaag cggggataac tactatagcg atccatttca aaatatcaaa
     1381 ggttctattg ttcatactat ccacctccct tcattggttg ggctactttc tttttcaatc
     1441 gcttgttttc ttctatcaat atttctcttt ctgaaagcaa ggcatcaatt tgcccgtcca
     1501 gttgttcaat tttatc
//
LOCUS       DACCDF010000186         1456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCDF010000186 DACCDF010000000
VERSION     DACCDF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.196"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            108..1040
                     /locus_tag="IVK51_002845"
     CDS             108..1040
                     /locus_tag="IVK51_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP6432352.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1456
                     /locus_tag="IVK51_002846"
     CDS             1112..>1456
                     /locus_tag="IVK51_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432353.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagat
//
LOCUS       DACCDF010000187         1433 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCDF010000187 DACCDF010000000
VERSION     DACCDF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.197"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(175..645)
                     /locus_tag="IVK51_002847"
     CDS             complement(175..645)
                     /locus_tag="IVK51_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432354.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            732..1430
                     /locus_tag="IVK51_002848"
     CDS             732..1430
                     /locus_tag="IVK51_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP6432355.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
BASE COUNT          515 a          191 c          287 g          440 t
ORIGIN      
        1 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
       61 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
      121 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
      181 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
      241 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
      301 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
      361 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
      421 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
      481 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
      541 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
      601 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
      661 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
      721 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
      781 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
      841 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
      901 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
      961 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
     1021 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
     1081 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
     1141 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
     1201 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
     1261 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
     1321 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
     1381 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agc
//
LOCUS       DACCDF010000188         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCDF010000188 DACCDF010000000
VERSION     DACCDF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.198"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(150..1337)
                     /locus_tag="IVK51_002849"
     CDS             complement(150..1337)
                     /locus_tag="IVK51_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP6432356.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCDF010000189         1414 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCDF010000189 DACCDF010000000
VERSION     DACCDF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.199"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>1414
                     /locus_tag="IVK51_002850"
     rRNA            <1..>1414
                     /locus_tag="IVK51_002850"
                     /product="23S ribosomal RNA"
BASE COUNT          380 a          304 c          430 g          300 t
ORIGIN      
        1 ggaaatgtct gtccaagcag taagtctgaa gaggagtcaa atgcttcttt tcttaaggac
       61 aagctgtgat ggggagggaa ataatagtac cgaagttcct gatgtcacac tgccgagaaa
      121 agcttctagt gagaaaacag cggcccgtac cgcaaaccga cacaggtagt cgaggagaga
      181 atcctaaggt gagcgagaga actctcgtta aggaactcgg caaaatgacc ccgtaacttc
      241 gggagaaggg gtgctgatca tacgatcagc cgcagtgaat aggcccaagc gactgtttat
      301 caaaaacaca ggtctctgca aaatcgtaag atgaagtata ggggctgacg cctgcccggt
      361 gctggaaggt taagaggagt gcttagcgca agcgaaggta cgaattgaag ccccagtaaa
      421 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      481 ccgcacgaaa ggcgtaacga tttgggcact gtctcaacga gagactcggt gaaattttag
      541 tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga gctttactgt
      601 agtttgatat tgagtgtctg taccgcatgt acaggatagg taggagccgt agaaatcgga
      661 acgctagttt cgatggaggc gctggtggga tactacccct gcgttatggc cactctaacc
      721 cgcaccacta atcgtggtgg gagacagtgt cagatgggca gtttgactgg ggcggtcgcc
      781 tcctaaaagg taacggaggc gcccaaaggt tccctcagaa tggttggaaa tcattcgaag
      841 agtgtaaagg cagaagggag cttgactgcg agaccaacaa gtcgagcagg gacgaaagtc
      901 gggcttagtg atccggtggt tccgcatgga agggccatcg ctcaacggat aaaagctacc
      961 ctggggataa caggcttatc tcccccaaga gtccacatcg acggggaggt ttggcacctc
     1021 gatgtcggct cgtcgcatcc tggggctgta gtcggtccca agggttgggc tgttcgccca
     1081 ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtcc ctatccgtcg
     1141 cgggcgttgg aaatttgaga ggagctgtcc ttagtacgag aggaccggga tggacttacc
     1201 gctggtgtac cagttgttct gccaagggca ttgctgggta gctatgtagg gaagggataa
     1261 acgctgaaag catctaagtg tgaagcccac ctcaagatga gatttcccat ttctttaaga
     1321 aagtaagatc cctgagagat gatcaggtag ataggtcagg agtggaagta cagtgatgta
     1381 tggagcggac tgatactaat cgatcgagga ctta
//
LOCUS       DACCDF010000190         1409 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCDF010000190 DACCDF010000000
VERSION     DACCDF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.200"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1359
                     /locus_tag="IVK51_002851"
     CDS             <1..1359
                     /locus_tag="IVK51_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP6432357.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACCDF010000191         1393 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCDF010000191 DACCDF010000000
VERSION     DACCDF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.201"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            132..473
                     /locus_tag="IVK51_002852"
     CDS             132..473
                     /locus_tag="IVK51_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432358.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            466..1137
                     /locus_tag="IVK51_002853"
     CDS             466..1137
                     /locus_tag="IVK51_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP6432359.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            1143..>1393
                     /locus_tag="IVK51_002854"
     CDS             1143..>1393
                     /locus_tag="IVK51_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432360.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFW"
BASE COUNT          512 a          229 c          286 g          366 t
ORIGIN      
        1 aaatactata aaccaatcat cctaagccaa caacatattg acaagcaaaa agcatggcaa
       61 caaataaaaa agtgactccg ccggcaagca aagagtcaca aagaaaacac atcataagga
      121 gattttagca tatggaaaaa gaactttcca ctctagatca atatttgatt gatcctgatt
      181 ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat cagacgaaat cttttgacga
      241 atgaagaact agcttgggat caagatgatt taggcaacca tgtaactatt tgggatcatg
      301 tttatcttat ccatctatcg aagcattcga ataaacctga atatatttac gtcatcgaag
      361 atggcttgat tgatgcgcta gaagagtacg acagagataa cttgattgat atctcttatt
      421 acggaccagg taagaaatac attgctgaaa tggaggcaga atttgatgag tgaaatcaaa
      481 gggacaacga actttgaaaa actttttagt cgtaagttaa ataaaattct caagaaaaaa
      541 ggaaattttg attatttatc ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca
      601 caggcaacgg taactattaa tgagtacaaa cactacaggg ttgtttctgg aactcatcaa
      661 gactttcttg ttgaggaata taaacctttt cttatggatg aaactgggac ttatgtatct
      721 gtctcagtaa cggttaaagg acacacggaa accgaattat ttcctgtttt agattatcga
      781 aaccaaccag ttgttaaacc aaatgcaatg caaatcaata actcattgaa gcgatgcttt
      841 gtgaaagcat tggctctaca cggactggga ttatatgtat ttcaagggga agatattcca
      901 acaccaccta gaatcgatac aaagaaatta aacatgctag agacgattct agaagctttc
      961 aatgagcaga tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca
     1021 gataaattag ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa
     1081 tgtgccttga tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat
     1141 gagtgtttaa accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag
     1201 acataagcgc aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg
     1261 gctacgatgg tgatcgagtc attgaaattc gttttattga tcctagacgt ttcaccgtac
     1321 agcaacgaaa cttcatctat gcgctgatag gcgatatttt tatcgataca ggcatgccaa
     1381 cggacttctg gaa
//
LOCUS       DACCDF010000192         1354 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCDF010000192 DACCDF010000000
VERSION     DACCDF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.202"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>1354
                     /locus_tag="IVK51_002855"
     CDS             82..>1354
                     /locus_tag="IVK51_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP6432361.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          460 a          261 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DACCDF010000193         1311 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCDF010000193 DACCDF010000000
VERSION     DACCDF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.203"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            200..1248
                     /locus_tag="IVK51_002856"
                     /pseudo
     CDS             200..1248
                     /locus_tag="IVK51_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180785.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          532 a          148 c          254 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tcttgatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc t
//
LOCUS       DACCDF010000194         1215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCDF010000194 DACCDF010000000
VERSION     DACCDF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.204"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            414..947
                     /locus_tag="IVK51_002857"
     CDS             414..947
                     /locus_tag="IVK51_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP6432362.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCDF010000195         1195 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCDF010000195 DACCDF010000000
VERSION     DACCDF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.205"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1076
                     /locus_tag="IVK51_002858"
     CDS             <1..1076
                     /locus_tag="IVK51_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6432363.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACCDF010000196         1188 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCDF010000196 DACCDF010000000
VERSION     DACCDF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.206"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            141..398
                     /locus_tag="IVK51_002859"
     CDS             141..398
                     /locus_tag="IVK51_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432364.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVK51_002860"
     CDS             complement(630..1043)
                     /locus_tag="IVK51_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP6432365.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          453 a          151 c          148 g          436 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatcc
//
LOCUS       DACCDF010000197         1156 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCDF010000197 DACCDF010000000
VERSION     DACCDF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.207"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(273..1154)
                     /gene="galU"
                     /locus_tag="IVK51_002861"
     CDS             complement(273..1154)
                     /gene="galU"
                     /locus_tag="IVK51_002861"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP6432366.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          294 a          259 c          180 g          423 t
ORIGIN      
        1 aaaaaattcg cctttttatt ctactatgaa tcttctggct tggctttcat ttctcttatg
       61 taatcatttt cttttataca acttaaaaaa aaattaaacc attagcataa tattttctga
      121 atctattcac gaaagtcttt ttctatcttt cgttttccac gactcaactg atctagagtc
      181 aaaaaactaa aacgacagtt gcgcaaatga cgcacaagct gccgctttaa taacaaatag
      241 cctttcattc tcagcaggcc acttatttcc agttaacttt ctgttcttag ctttttactc
      301 aactggataa tatatgcact taatgaagtc ccaatttccg aatgttgaag tccatattca
      361 atatttgtct tcatatatcc taacttatcg cctacgtcgt atctttttcc tttaaactct
      421 ctcgcataga ccgcctctgt ctggttaaga gtctgaagtg catccgttag ctgtatctcg
      481 tttcctgctc ctggttcttg agtttctaat atctcaaata ttttaggagt cagtagataa
      541 cgtccgataa tggctaaatt acttggtgct tttcccattt caggtttttc tacaaaccct
      601 tttactctgc aacgaccctt ttcgctatct gttttaggat caataattcc gtatttagat
      661 gtttcatttt ctggtacacg catgacggca acagcggaag cttgtgtttc ttcataatcc
      721 atcatcaatt gttgagtcaa tgggatatca tctttcatca aatcatcccc cagcatgacg
      781 acaaacggtt catttcctat aaatgctttt gcttgaagca cggcatcccc cagtcctctt
      841 ggatgagatt gacggatgaa gtgaagattg acttgagtcg tttcttcaac taaagcgagt
      901 aattctgttt tttccttttc ccgcaaattg ttttccaatt cgatatttgc atcaaagtga
      961 tcttcaatgg gtcttttttc ttttcccgtt acgatcaaga tgtcttcgat tcccgaatcc
     1021 aatgcttctt ccacaataaa ctggatcgtt ggtttatcca cgattggaag catttctttt
     1081 gccatggctt ttgttgccgg caaaaaacgt gttcctagcc cagcggcagg aatgactgct
     1141 tttttgacct tcactt
//
LOCUS       DACCDF010000198         1091 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCDF010000198 DACCDF010000000
VERSION     DACCDF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.208"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            6..980
                     /locus_tag="IVK51_002862"
     CDS             6..980
                     /locus_tag="IVK51_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP6432367.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          384 a          135 c          187 g          385 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaaact aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt
     1081 aatgttttta a
//
LOCUS       DACCDF010000199         1053 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCDF010000199 DACCDF010000000
VERSION     DACCDF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.209"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..267
                     /locus_tag="IVK51_002863"
     CDS             1..267
                     /locus_tag="IVK51_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432368.1"
                     /translation="MAGSITAIVSLILLVIKPITASFSKITETLSKVSHNLDLLTKDL
                     ESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            264..488
                     /locus_tag="IVK51_002864"
     CDS             264..488
                     /locus_tag="IVK51_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP6432369.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            485..>1053
                     /locus_tag="IVK51_002865"
     CDS             485..>1053
                     /locus_tag="IVK51_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432370.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLA"
BASE COUNT          365 a          167 c          216 g          305 t
ORIGIN      
        1 atagcaggat caataaccgc tatcgtttct ttgattttat tagtaataaa accaattact
       61 gcatctttct cgaagattac tgagactctt tcaaaagtaa gtcacaattt agatttgctg
      121 actaaagatt tagaatcgag caaatcagat cgattgatga ttcatgaaga actaaagaaa
      181 cacgatgaaa gattagatac acatgcagaa aaattggtag aacacacgca acaaattaaa
      241 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
      301 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
      361 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
      421 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
      481 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
      541 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
      601 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
      661 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac
      721 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
      781 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
      841 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
      901 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
      961 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
     1021 atcgcaaatg ggagttagca aagaaaagct tgc
//
LOCUS       DACCDF010000200         1020 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCDF010000200 DACCDF010000000
VERSION     DACCDF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.210"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..398
                     /locus_tag="IVK51_002866"
     CDS             <1..398
                     /locus_tag="IVK51_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432371.1"
                     /translation="FDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDDKYRFEVL
                     KIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVM
                     ITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            504..767
                     /locus_tag="IVK51_002867"
     CDS             504..767
                     /locus_tag="IVK51_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP6432372.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            767..>1020
                     /locus_tag="IVK51_002868"
     CDS             767..>1020
                     /locus_tag="IVK51_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP6432373.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          394 a          122 c          203 g          301 t
ORIGIN      
        1 cgtttgattt tcaaacttcg caacagaacc gaaaatttat accggaagat tttcaaaact
       61 tacagttaga aaacatgcgt ttagaagatt tatttagatt agcagatgat gataagtata
      121 gatttgaagt tttgaaaatt aaaaagaatg aactattgaa taagttatat cctgagaatg
      181 cgataaaagc acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat
      241 ttagagggaa aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg
      301 tcgtaatgat aacagatatc tacgtagaag atgaagttat aatcaaatat atgaagcctg
      361 tgataaaact tgttaataaa aataaaggaa acggataaca ctttacaatg ctgttttggt
      421 actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt agaatggaga
      481 tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc tcagcacgga
      541 aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt gaaatcatta
      601 gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgatttgaaa gcaatcaaag
      661 aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga gaaaaagatg
      721 atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga ataactattc
      781 tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt ctaatttgaa
      841 acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc ctacccaatc
      901 atttgagaag ttaaaaccta catatgaagg taggtattct agaagattga atagacaaca
      961 ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt cagcaaggac
//
LOCUS       DACCDF010000201          994 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCDF010000201 DACCDF010000000
VERSION     DACCDF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.211"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>994)
                     /locus_tag="IVK51_002869"
     rRNA            complement(<1..>994)
                     /locus_tag="IVK51_002869"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACCDF010000202          953 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCDF010000202 DACCDF010000000
VERSION     DACCDF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.212"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..226
                     /locus_tag="IVK51_002870"
     CDS             <1..226
                     /locus_tag="IVK51_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432374.1"
                     /translation="VAEVQKLGKSMWTEKPKRRTPRKIAIWHDDRILVTGTAEQLSEI
                     TGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            226..582
                     /locus_tag="IVK51_002871"
     CDS             226..582
                     /locus_tag="IVK51_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP6432375.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            542..787
                     /locus_tag="IVK51_002872"
     CDS             542..787
                     /locus_tag="IVK51_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432376.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            784..>953
                     /locus_tag="IVK51_002873"
     CDS             784..>953
                     /locus_tag="IVK51_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP6432377.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTG"
BASE COUNT          366 a          136 c          219 g          232 t
ORIGIN      
        1 agttgcagaa gtccaaaagc tcggtaaatc aatgtggact gaaaagccca aacggagaac
       61 gccgagaaaa attgcaatct ggcatgatga ccgaatttta gtaacaggta ctgctgaaca
      121 gttatctgaa attactggat taagcaaaaa cattatctgg gatagagcta ggagcttatg
      181 gattgattca aaaggacgac agtttaggta tgtggaggag agataatgga tctcattaca
      241 caatacagtg acatcatcct caagaaaatc atgatgaaga ttcagaaaga taaaaaatca
      301 aaagaacgag ctgaattagt taagttagaa atggctgaaa caggagcagg agtgcgaagt
      361 agcaggcatt ggaaagcagc agcaaacatt gaattttatt acaacgaaat tcaaaaaggg
      421 ttcgatcaga tgcgtgagct ggatcggcaa acaaattgga gcaagaaact tcatcaagat
      481 cgtttcaaat ttgtagagaa gtataaagaa atattagaag agtatttgag gaggacagca
      541 aatgataaaa aaactcgttc aattcagcat ggatttatat gatatcgaat caggagcaac
      601 actatctgtg gaatcggacc atctaatcat aaattttggt ggaaagcgcc agattatttt
      661 gtgggtagtt gatgatgtac tgtttccaga aattgttcat gatttcgaag aatcaaaagc
      721 ggttgagttt gaaatagtga aaaaagtaat ggaattgatt gaaaaatacg aggaggacag
      781 cgaatgatac cgaagtttag agcgtgggat aaacgaaaga acgtaatgag agatgtagcc
      841 gtcttgcatt ttactaaaaa cggcaaaaca aactttattg aatattggat aaatcctacc
      901 gaattgaaat catatcacgt gcgaaacatc gacctcatgc aatccacagg aat
//
LOCUS       DACCDF010000203          860 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCDF010000203 DACCDF010000000
VERSION     DACCDF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.213"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..856
                     /locus_tag="IVK51_002874"
     CDS             <1..856
                     /locus_tag="IVK51_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6432378.1"
                     /translation="KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHA
                     LLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFH
                     IVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRA
                     SFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPET
                     YTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIR
                     HGKTFLTK"
BASE COUNT          273 a          173 c          165 g          249 t
ORIGIN      
        1 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
       61 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      121 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      181 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      241 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      301 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      361 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      421 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      481 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      541 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      601 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      661 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      721 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      781 gtacggcttt cgcaactttt acaatttcaa actacgaatt atgattcgtc atggcaaaac
      841 atttttaacc aaataaacag
//
LOCUS       DACCDF010000204          841 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCDF010000204 DACCDF010000000
VERSION     DACCDF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.214"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IVK51_002875"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IVK51_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP6432379.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="IVK51_002876"
     CDS             complement(591..785)
                     /locus_tag="IVK51_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432380.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          244 a          168 c          146 g          283 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 t
//
LOCUS       DACCDF010000205          791 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCDF010000205 DACCDF010000000
VERSION     DACCDF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.215"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..782
                     /locus_tag="IVK51_002877"
     CDS             <1..782
                     /locus_tag="IVK51_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP6432381.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          340 a          107 c          102 g          242 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc t
//
LOCUS       DACCDF010000206          787 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCDF010000206 DACCDF010000000
VERSION     DACCDF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.216"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            24..716
                     /locus_tag="IVK51_002878"
     CDS             24..716
                     /locus_tag="IVK51_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP6432382.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          282 a          151 c          159 g          195 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 cttttat
//
LOCUS       DACCDF010000207          785 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCDF010000207 DACCDF010000000
VERSION     DACCDF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.217"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..582
                     /locus_tag="IVK51_002879"
     CDS             <1..582
                     /locus_tag="IVK51_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HAP6432383.1"
                     /translation="PTESTYVWNELLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPK
                     AKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASSKEEANTFLGSMIEKWQKTYP
                     KVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQIKKYSRRKEQFQNEESLERF
                     LVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          275 a          138 c          133 g          239 t
ORIGIN      
        1 ccgactgaat cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt
       61 catgatgtct tactgtttat taccgatggc ttaaaaggca tgaaagatac gattcaccag
      121 atttatccta aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tattgctcat
      181 aaagtacgtg tcaaagatcg aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct
      241 agctcaaagg aagaggcaaa taccttttta gggagtatga ttgagaagtg gcagaaaact
      301 tatcctaaag tgacgcagtc actgataaaa aatcaagatt tattgacttt ttatgagttt
      361 ccgcctggta tccgccgaag catttactca actaatttaa tcgaatcttt caataaacaa
      421 atcaagaaat acagccgcag aaaagagcag tttcaaaatg aagaatcact agagcgtttc
      481 ctagtatcca tctttgatac atacaatcaa aaattcttaa atagaagcca taaaggcttc
      541 caacaagtga cggatacatt agcttcaatg tttactgagt aacccattat tttgcagaag
      601 gacgagttat ttacacaaaa ttattgacgc tcccttttat aagcaattta gttaacataa
      661 tgtaataagg tagaaatgtt taaatactga aaataaatta caattgtatt accaaactag
      721 tttacgctgc ttttgattac aaattagcta ataatatgcg ctaatttata tatgtaccgc
      781 ccctc
//
LOCUS       DACCDF010000208          775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCDF010000208 DACCDF010000000
VERSION     DACCDF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.218"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..228
                     /locus_tag="IVK51_002880"
     CDS             <1..228
                     /locus_tag="IVK51_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP6432384.1"
                     /translation="IFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCATVDEDLE
                     YQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            225..>775
                     /locus_tag="IVK51_002881"
     CDS             225..>775
                     /locus_tag="IVK51_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP6432385.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVV"
BASE COUNT          277 a          111 c          180 g          207 t
ORIGIN      
        1 atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta cttagataat
       61 aaagtctgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc cactgttgat
      121 gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac gtacgaagtc
      181 accggaaata tatacgagaa tagcgagtta ttggaggaac agcgatgaat aaacaggaag
      241 caatcaaaaa gttagaaagt attaaagcga taggaaatga tgcaatagct gcttgctata
      301 acgagagtat aaattcaggt attacgttaa tgaaaaaaat agacgaaccg cagaaaccag
      361 ttattccaca actcgtggcc ggttggcttg agaaatctac ggaccctttt acaaaagctg
      421 aaaaaatagc gtatttaatc aaatctaaag atggtgattc atattatttc tgtgattggt
      481 ttgtacgaga tggcatagtg acgcaagagc aaggagaaga attactcgct tgggcaacaa
      541 gacaatcata tgaaacacta ttgagcctat acaacggcta cgaggttgag aaagagccgt
      601 tatgggcaat aaagaatgcc gatggaaact atcttactaa atgtgcttta tggggaaaag
      661 atggagtaaa ttatagtttt gaatgcaatc catctcatcg attgcttttc actgataaag
      721 caacagcgga tgctgcagca ttgttggtga atggaacagt ggaagaggtg gtaga
//
LOCUS       DACCDF010000209          774 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCDF010000209 DACCDF010000000
VERSION     DACCDF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.219"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(248..772)
                     /locus_tag="IVK51_002882"
     CDS             complement(248..772)
                     /locus_tag="IVK51_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432386.1"
                     /translation="MIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVE
                     LDVNNYRELYVFNSLDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKGKKIKKCDKC
                     FESAVVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQISSNLEDIKK
                     AFKIKTIGTAPNFK"
BASE COUNT          262 a          106 c           87 g          319 t
ORIGIN      
        1 gacgctccct taacaataaa tgaagacttt tttattgtta attgtttatt tttctattat
       61 tagatatgaa aatcgcattt agataaaata gaaaaatatg aatatatttt aaatattaag
      121 atgagagtca ttaacttatg atagtagtca atttgctttt cactaattac aaaatatgtg
      181 atataggaag tttagttatt gttctcataa tgccatcctg tttttagatt atctagtaat
      241 ttttttgtta tttaaaatta ggagcagtgc caattgtttt aattttaaag gcttttttta
      301 tatcttctaa attagaagat atttgtatat caatatggaa tatattacct tttttgtatt
      361 tttttatgtt atctttagct aaaacatcaa atacatgtcc ttgaaattgt atcgaggcag
      421 ctggatttag tttaaattga ggaaacttta ccacagctga ttcaaaacac ttgtcacatt
      481 tttttatttt tttacctttg taaaaatatt cgattgaata ttctatttgg cacgactcac
      541 ttggcagtat atgtatgtca atatttttca tctgtttcat aggtatttca aaatctaaac
      601 tattaaaaac atataattcc ctgtaattat ttacatctaa ctctactaaa tttatctttg
      661 gaacaataga tcctccaccc attccaccat aataaaaaaa atcttgttct ctatcaaaat
      721 agcacatatt aagctcagtt tgtttatcat cctcaataat tttataaatc aaac
//
LOCUS       DACCDF010000210          741 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCDF010000210 DACCDF010000000
VERSION     DACCDF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.220"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>741
                     /locus_tag="IVK51_002883"
     rRNA            <1..>741
                     /locus_tag="IVK51_002883"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          151 c          220 g          144 t
ORIGIN      
        1 caaagagggt aaaaaccccg tagacgaaat gtggaagaca cctaggagga tcctgagtac
       61 ggcggaacac gagaaattcc gtcggaatcc gggaggacca tctcccaagg ctaaatactc
      121 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccggaagggg
      181 agtgaaatag aacctgaaac cgtgtgccta caacaagtca aagcccgtta atgggtgatg
      241 gcgtgccttt tgtagaatga accggcgagt tacgattgca tgcgaggtta agttgaagag
      301 acggagccgc agcgaaagcg agtctgaata gggcgtttga gtatgtagtc gtagacccga
      361 aaccatgtga tctacccatg tccaggttga aggtgcggta aaacgcactg gaggaccgaa
      421 cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag cggagaaatt ccaaacgaac
      481 ttggagatag ctggttctct ccgaaatagc tttagggcta gcctcggaat tgagaatgat
      541 ggaggtagag cactgtttgg actaggggcc catctcgggt taccgaattc agataaactc
      601 cgaatgccat tcattcatat ccgggagtca gactgtgagt gataagatcc atagtcgaaa
      661 gggaaacagc ccagaccacc agctaaggtc ccaaaatata tgttaagtgg aaaaggatgt
      721 ggggttgcac agacaactag g
//
LOCUS       DACCDF010000211          708 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCDF010000211 DACCDF010000000
VERSION     DACCDF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.221"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            14..538
                     /locus_tag="IVK51_002884"
     CDS             14..538
                     /locus_tag="IVK51_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432387.1"
                     /translation="MADIVQLKEDGVAKYLKTHAKGIDGVEGVLVKATGNETVLGTKN
                     FKDGLQFNGLPVQAGMIERAITLADRSDTTNVTDVNGKIIRIGNIVFLTFNFKCGTWP
                     EGSETRWILKIPDGFKRDQGYPAQTALSLVRNASQPADARAFIDQSSIIQAKSGSGSS
                     YISGMWITQDPWPA"
BASE COUNT          242 a          114 c          163 g          189 t
ORIGIN      
        1 tggggacggt ttaatggctg atattgtgca attaaaagaa gacggagttg ctaagtattt
       61 gaaaactcat gccaaaggaa ttgatggtgt ggagggtgtt ttagtaaaag ccacaggtaa
      121 tgaaacggtc ttaggaacga aaaattttaa agacggattg caattcaatg gattgcctgt
      181 gcaagcaggt atgatcgagc gagctatcac actagctgat cgaagtgata caacaaacgt
      241 aactgatgtg aatggaaaaa taatcagaat cggaaacatc gtatttctta cttttaattt
      301 taaatgtggc acatggcccg aaggatcaga aactcgttgg attttaaaaa tcccggacgg
      361 tttcaaacgt gatcaaggct atccagcgca aaccgcgctt tcactagtta gaaatgcaag
      421 tcagccagcg gatgcacgtg cattcattga tcaaagtagc atcatacaag ccaaatcagg
      481 ttcaggtagt tcatatattt caggtatgtg gatcactcaa gatccttggc cggcttaaca
      541 gattactaat tggaggaaga gtaaaatgaa aacaatttat aaagtattgt atccaatggg
      601 atatgaagaa catcaagtag aagataactt tccaacatct ttaccatttg tcgaagttcc
      661 acctattttg tttgaaaaga aggaagatga aacggatgaa ggtttcgg
//
LOCUS       DACCDF010000212          637 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCDF010000212 DACCDF010000000
VERSION     DACCDF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.222"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            74..403
                     /locus_tag="IVK51_002885"
     CDS             74..403
                     /locus_tag="IVK51_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6432388.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..>637
                     /locus_tag="IVK51_002886"
     CDS             393..>637
                     /locus_tag="IVK51_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6432389.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCG"
BASE COUNT          244 a           70 c          123 g          200 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtgg
//
LOCUS       DACCDF010000213          621 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCDF010000213 DACCDF010000000
VERSION     DACCDF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.223"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>621
                     /locus_tag="IVK51_002887"
     CDS             <1..>621
                     /locus_tag="IVK51_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432390.1"
                     /translation="QLPFLKRYFSHFSREIREAVEWIVIDMYAPYVSLVKKLFPKAQL
                     IIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLDYT
                     RRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTNNI
                     MLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNA"
BASE COUNT          210 a          129 c          110 g          172 t
ORIGIN      
        1 caactgcctt ttttaaaacg ttattttagt catttttcac gagaaattcg tgaagcggtc
       61 gaatggatcg tgattgatat gtacgctcct tatgtatcgc tcgttaaaaa gctgtttcca
      121 aaagcacagc tgattattga ccggtttcac atcgtccaac atattggtcg tacctttcgc
      181 aatcatcgaa tcaaagaaac gaaccaacta ttaaaaagca aagaacaaaa acactatcaa
      241 ttaggtaagc aattaaaaag atactggaaa cttcttcaaa aagatgaacg caagcttgat
      301 tatactcgac gcctttggcg acctggcttc aaagctcatt taacagagac cgatattgtg
      361 gatcgcttac tgaaagggag ccctgctttg cgtgtaggct atcagttata tcaagacttt
      421 ctttacgctg taaaggaacg agactacgta tcgtttgaag aactacttac caataatatc
      481 atgcttcccg aaggctatca gacgaccttg agaacgttcc agaaatttct gccacaaatc
      541 aaaaatgctt tacaacaaag ttattcaaat ggcccacttg aatgtctgaa caaccatatc
      601 aaagtgttaa aacgaaatgc a
//
LOCUS       DACCDF010000214          614 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCDF010000214 DACCDF010000000
VERSION     DACCDF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.224"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..308)
                     /locus_tag="IVK51_002888"
     CDS             complement(<1..308)
                     /locus_tag="IVK51_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6432391.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          195 a          119 c           86 g          214 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgccc
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca cgtcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccgattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataaggaa
      421 aaggtagtac ataaataagg aaaagctgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aattttttca actg
//
LOCUS       DACCDF010000215          614 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCDF010000215 DACCDF010000000
VERSION     DACCDF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.225"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..308)
                     /locus_tag="IVK51_002889"
     CDS             complement(<1..308)
                     /locus_tag="IVK51_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP6432392.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     "
BASE COUNT          197 a          117 c           83 g          217 t
ORIGIN      
        1 tctgctggaa gtatatagag gttattgatt actttataga cagcctcttt aggtggaaga
       61 ttgtttatca acacgtcagt cacagtattt tcgtaaacaa tcggttcacc ttctccgcct
      121 ccttcttcgt tagtgaagtt catatgcgtt aagttcatca catcagaagt atttccttgc
      181 atatccaagt caattatcaa ggttcttttt ccttcattaa ctgctgattc aaaccctaac
      241 atttgcgcgc acgtactttt acctacgcca cctttaaaat taccaattac atatttcttc
      301 gctaccacgt tcctacctct tttcttttag caatattcat ttgatactat tataacatat
      361 ttatttttag ttactaccaa aaaaaagata tatatgtact accttataat taataagtaa
      421 aaggtagtac ataaataagg aaaagttgaa atatattgat ttatagcttt cctcaaagta
      481 atgacgttat ttaatttatc gagaagcaga gaatactcgc gactttagtc atgtagacga
      541 atcagatttt cgataaaaaa gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt
      601 aattttttca actg
//
LOCUS       DACCDF010000216          605 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCDF010000216 DACCDF010000000
VERSION     DACCDF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.226"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>605
                     /locus_tag="IVK51_002890"
     CDS             <1..>605
                     /locus_tag="IVK51_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432393.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFN"
BASE COUNT          173 a          129 c          137 g          166 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataa
//
LOCUS       DACCDF010000217          604 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCDF010000217 DACCDF010000000
VERSION     DACCDF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.227"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            35..>604
                     /locus_tag="IVK51_002891"
     CDS             35..>604
                     /locus_tag="IVK51_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6432394.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIIT"
BASE COUNT          207 a          112 c          115 g          170 t
ORIGIN      
        1 gttccgacca aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga
       61 tgaacttgta ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca
      121 ttgcttgaat cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa
      181 atcagcctta gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc
      241 gcttgtttta cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac
      301 acccgatgtg attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta
      361 tcgtatgttc aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa
      421 accgaatgga catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg
      481 tgaaaaggat tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt
      541 aggtctgaaa cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat
      601 caca
//
LOCUS       DACCDF010000218          603 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCDF010000218 DACCDF010000000
VERSION     DACCDF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.228"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..246
                     /locus_tag="IVK51_002892"
     CDS             <1..246
                     /locus_tag="IVK51_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432395.1"
                     /translation="MLDMPTKNPKTRKKSKFKVGDMVRCEAEEFVYPFRGYVKKILSN
                     SAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            323..>603
                     /locus_tag="IVK51_002893"
     CDS             323..>603
                     /locus_tag="IVK51_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP6432396.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVP"
BASE COUNT          202 a          107 c          143 g          151 t
ORIGIN      
        1 atgctagata tgccgacaaa gaaccctaaa acacgcaaga aatcaaaatt caaagtcggt
       61 gacatggtcc gctgtgaagc agaagagttc gtttatccgt tcagaggata cgttaaaaag
      121 atactgtcaa actcagcaat cattcgtatt gaaaatacaa tgaagtgtga tcgttcgact
      181 gcaaaacaca agcattattt agctgtagcg agattagttg acatggaagt aatcaagagc
      241 aaataaaaaa agccggatcg ctccgactga ttcaataaat ccaacacatt tattatatca
      301 cataaaggag cggtttgact tgatgcaatt gttacgagag gtagatttca aacagacaag
      361 atgtaatgcg agagatgtgc tgaagaactt tcggcgtttg gagcggatgg caggtcgctc
      421 tttgatagat attaagtcgc cgattataac ggatatgccg aaggcaccga agcatggcaa
      481 taaggcagaa gacgcgatta ttcagatgat ggatatagaa acggagagag atgcgattct
      541 agcggctttg atggctctta gtctgattag tcgtcagata ctctactaca gcttctgtgt
      601 gcc
//
LOCUS       DACCDF010000219          602 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCDF010000219 DACCDF010000000
VERSION     DACCDF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.229"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(318..581)
                     /locus_tag="IVK51_002894"
     CDS             complement(318..581)
                     /locus_tag="IVK51_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HAP6432397.1"
                     /translation="MKKNLLLVLPILGFAGFFVGVPMLSANIGISSYAAKKVIDIINT
                     GSTVATIISIVAAVVGGGLITAGIVATAKSLIKKYGAKYAAAW"
BASE COUNT          226 a          104 c           79 g          193 t
ORIGIN      
        1 gccttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcctgaatt
       61 ttttcatgtt gaaaacatca ctaatcaaag gcaaattata atttagaaat agaatgaaaa
      121 cactaaatga aaatagaaca ttagaaaaaa tagaatcaaa aaaacccaag actagtgccg
      181 aaataaggga aacaatcgaa gctacaaaaa ataatgattt cgttgaacag gaccaattta
      241 tttgtttccg atatttctca aaaagaactt tctttgggaa agttaaaggc gactgtaaag
      301 tcgcctttag tcctttttta ccaagctgct gcatatttag ctccatattt tttaattaat
      361 gatttggctg tagcaacaat accagctgta attaatccgc ctccaacaac tgcagcaaca
      421 atagaaatga ttgtagcaac cgtgctacca gtattaatta tgtcaataac ttttttagct
      481 gcataagaag aaattccaat gttagcactt agcattggta caccaacaaa gaatcccgca
      541 aaacctaaaa ttggtaaaac taataataaa ttctttttca taataattcc tctatttttt
      601 ta
//
LOCUS       DACCDF010000220          587 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCDF010000220 DACCDF010000000
VERSION     DACCDF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.230"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            26..412
                     /locus_tag="IVK51_002895"
     CDS             26..412
                     /locus_tag="IVK51_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP6432398.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT          221 a           93 c          118 g          155 t
ORIGIN      
        1 ataaaaataa aaaaggagat ctttcatgaa aaaaatttta gggttgatcg ttatcatatt
       61 gattgctttt ataggatgga aaggttggga ctactatcag tctacttatg taggtaaaga
      121 ttattatgcg gtagtcaaag ctccaatgcc tgcagaaact gatatcaaag cagataatgg
      181 ggaagtaatt ggaagaggtt ttaaatacaa cgtagatgct tatgctgaaa acggtgaaaa
      241 aagacagtta gatttcgatg tcattacttc tggtgactct gccaatggtt ctgcttaccc
      301 cgaaggaacg atcttgcagt tgaaagcaag cgaaaagcgg atcatagaga aaaaagtaat
      361 taccagcgat aaggtaccct catctgtaaa agaaaaatta ggcctatctt aagctaagaa
      421 tatatcgcaa aaaagcactc gaagtgagtg cttttttgct tactgattct agaaaacaaa
      481 aaaacagaat taaccttcga agtcagcctc actgttctta aagtcctaaa acaaactaaa
      541 gagcaaggca atcaatcgat ttctatacaa acaagggact gtgacaa
//
LOCUS       DACCDF010000221          577 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCDF010000221 DACCDF010000000
VERSION     DACCDF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.231"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..417
                     /locus_tag="IVK51_002896"
     CDS             <1..417
                     /locus_tag="IVK51_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432399.1"
                     /translation="TSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPS
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          197 a          108 c          112 g          160 t
ORIGIN      
        1 acttctgaaa acaagcctgt cattgatcca acacgagctg gtgcagctaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttgggg aaatcgaaaa cgcaaacttg
      121 catgtggctg gatggcacat tgctaactat aaatacgagt atattttcat tatggactac
      181 aatactggga aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaac
      241 caagcttata atacttctgg aaatgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctagtaaga aagtctatgt aatgatgcgg gcaacgaatg atccagaggg aaacactaaa
      361 ggcggtgcgc aagatttcca tgacaaacgt tggtatttaa atattcctaa acgataaaaa
      421 tagctcctcg ttgaggagca gtacataact atattgacaa ctataaaaat tattcgataa
      481 aatagtgatg ttatcgcata tcttcactat cacccataaa tagtcacact ccaagctatg
      541 cgataacagg tttgttgcca cacattctac tggttga
//
LOCUS       DACCDF010000222          575 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCDF010000222 DACCDF010000000
VERSION     DACCDF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.232"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            312..>575
                     /locus_tag="IVK51_002897"
     CDS             312..>575
                     /locus_tag="IVK51_002897"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6432400.1"
                     /translation="MSAVVTDLTYVRVGTNWAYICLMIDLFNREIIGHSCGNKKDAQL
                     VKRAIQSIPYSLQEIELFHTDRGKEFDNQTIQNLMNGLVIKFLS"
BASE COUNT          194 a          105 c           83 g          193 t
ORIGIN      
        1 ttttacactc tctattattc ttttcataaa tattgtaaat agaagtatta aaaatatata
       61 caaaaagtat tatttcgaga aagataaata ttttcaaaaa ccttttcata atcctcaccc
      121 tttcttacac tgataaatca tgcgtctaat attcaaatta tactcgatat gctttagcta
      181 atcagccaac tctgtttgtc tactatcatt atatcaatag acaatatctg ctcccgtctt
      241 tatgatcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga aagtttactc
      301 aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc gggacaaatt
      361 gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc cattcttgcg
      421 gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct tactcacttc
      481 aagaaatcga actcttccat acggatcgtg gaaaggaatt tgataaccaa accattcaga
      541 atctcatgaa tggtttggtt atcaaattcc tttcc
//
LOCUS       DACCDF010000223          574 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCDF010000223 DACCDF010000000
VERSION     DACCDF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.233"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..572
                     /locus_tag="IVK51_002898"
     CDS             <1..572
                     /locus_tag="IVK51_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP6432401.1"
                     /translation="IRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIK
                     GRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQY
                     QMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLN
                     EYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          248 a           71 c           94 g          161 t
ORIGIN      
        1 aaatccgaat aaagcgatat aaatcttata aaggaacagt aggaaaaatt gccaagaatg
       61 tgttaaaacg aaatttttca gtagatacac ccaataaaaa atgggtgaca gacgtcactg
      121 aattcaagat aaaaggaaga aaaatctatc tatctcctat acttgattta tttaacggag
      181 aaataattag ttatagtata tcaactagtc caacatataa actgattgaa gaaatgcttc
      241 aacaagcaat taaaaaaaag ggaactgagg gttcactgat tctacattca gatcaaggat
      301 ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga gaataacatt attcaaagca
      361 tgtctagaaa aggaaattgc ttagataatt ctgtaataga aaattttttt ggtgtgctta
      421 aatcagagtt tttttatcga gaaaaattcc gatcaattga gatatttcaa agtaaattaa
      481 atgagtatat taggtggtat aataacaaaa ggataaaact aaagttaaat ggactatctc
      541 cagtagaata tcggaaacag tccattaaat agtt
//
LOCUS       DACCDF010000224          560 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCDF010000224 DACCDF010000000
VERSION     DACCDF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.234"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>560
                     /locus_tag="IVK51_002899"
     CDS             <1..>560
                     /locus_tag="IVK51_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432402.1"
                     /translation="SGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF
                     QLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFL
                     YDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKNHQRVITRH
                     VWGDLMDEVEHLRLTDLNKSIYKKRK"
BASE COUNT          181 a          114 c          106 g          159 t
ORIGIN      
        1 agcggctact acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca
       61 tgcgacgaca atggatttat tcttagcaca atcatcactc ctggaaatat tcatgacagt
      121 caagtcgcct ttcagctcgt caaacaatcg aagagacttt ttcctgaaat caattgtgtt
      181 gtcgctgatg ctgggtataa gacacctaag tttgttcact ttttgactca tttgaacctt
      241 cgaccttgtt tgccttattc aaggcccaaa gggaaaaagg ggctattatc aaaaaatgag
      301 tttctctatg acgagtattt tgattgttat atttgtccgc aagatcaaat gctcgctttt
      361 tcaacggtta ctcgagaagg gtaccgagaa tacaaatcca atccaaaaga atgtgtgaac
      421 tgtcctctgc taaaccaatg taccataagt aaaaatcatc aacgagtcat cacgagacat
      481 gtttggggag atctgatgga tgaagtggaa catcttcgcc taacagatct gaataaatca
      541 atttacaaaa aacggaaaca
//
LOCUS       DACCDF010000225          534 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCDF010000225 DACCDF010000000
VERSION     DACCDF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.235"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..126
                     /locus_tag="IVK51_002900"
     CDS             <1..126
                     /locus_tag="IVK51_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP6432403.1"
                     /translation="IPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTAKGMGRLIG"
BASE COUNT          174 a           78 c          131 g          151 t
ORIGIN      
        1 atccccttaa aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg
       61 attggtcgaa gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc
      121 ggctgaattg atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt
      181 gtcgagactt ctaaaggaac ttagagtcac tagaaaaata aggagtagac ttgatctatc
      241 aacaattccc ttcggggagt gacggggtag gttttaacga ttggtaacgt aaaaaattgg
      301 tcaatcgagt aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataataagc
      361 gcagagtaaa caagagggaa tttagaagtt agacaagtta gaatagcgtc tagattaatc
      421 atgagcagct actaagggat ctagactttg acgcaaactc atggaataag gagtaggtct
      481 tctcgtaatg gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgta
//
LOCUS       DACCDF010000226          528 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCDF010000226 DACCDF010000000
VERSION     DACCDF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.236"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(5..90)
                     /locus_tag="IVK51_002901"
     tRNA            complement(5..90)
                     /locus_tag="IVK51_002901"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IVK51_002902"
     tRNA            complement(107..178)
                     /locus_tag="IVK51_002902"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IVK51_002903"
     tRNA            complement(190..262)
                     /locus_tag="IVK51_002903"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IVK51_002904"
     tRNA            complement(280..361)
                     /locus_tag="IVK51_002904"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IVK51_002905"
     tRNA            complement(369..441)
                     /locus_tag="IVK51_002905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IVK51_002906"
     tRNA            complement(445..517)
                     /locus_tag="IVK51_002906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACCDF010000227          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCDF010000227 DACCDF010000000
VERSION     DACCDF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.237"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..132)
                     /locus_tag="IVK51_002907"
     CDS             complement(<1..132)
                     /locus_tag="IVK51_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP6432404.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCDF010000228          491 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCDF010000228 DACCDF010000000
VERSION     DACCDF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.238"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          161 a           94 c           76 g          160 t
ORIGIN      
        1 gacactcctc ttaatataca gtcctattgt ataaagtgtt actatataca tagtaactgt
       61 atacataaaa aaagtcaagt attagaaaaa agttcaaaag atcaagtgtg cgcttcgctc
      121 ccactcccct cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa
      181 aagttttcgg gatacattct ccttctcttc ctaataatca attaaaattt taatctttat
      241 atttctgcta atcactcagg aaacagtaca gaaaaacaaa cacgtatcat agatataaat
      301 gtaatggcat agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt
      361 gtgtccgttt ttgggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgttct
      421 aagtcaaaaa actctgacag cgcctttata tagataaagt ccgtataatt gtgtaaaagt
      481 aaaaaggcca t
//
LOCUS       DACCDF010000229          488 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCDF010000229 DACCDF010000000
VERSION     DACCDF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.239"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..414)
                     /locus_tag="IVK51_002908"
     CDS             complement(<1..414)
                     /locus_tag="IVK51_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432405.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFF"
BASE COUNT          185 a           78 c           62 g          163 t
ORIGIN      
        1 aaaaaacggc tttccaaatg atgaaatacc taaatctaac ttattaagaa agttgaaatt
       61 tttatattca ggaaaccatt tattaatttc atgtgcagca cgtaatgcat tccctggagt
      121 tgtcaaagag aaaattaatt ctgctaagct tattattgaa aatactgata acttataatt
      181 atatcgtcca ttgacaatca aatataaact aactatactt gttagaacaa aaaaacaggc
      241 gttgacttgt tcttgattag ttaaaaatat caataaagga atacttaccc aatatatttt
      301 tctatttatt tcttctcctc ttaagagttg aaaaaacgga taaaaagtta gtaaactaaa
      361 tgctactggc cactgatagt tcaaggtggt tgctctccaa ccggtttccc ccattactgt
      421 caatggtatt aaacagtata tagagcaaac caataataaa tttttaacat ttattttctc
      481 aaaaacat
//
LOCUS       DACCDF010000230          487 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCDF010000230 DACCDF010000000
VERSION     DACCDF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.240"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>487)
                     /locus_tag="IVK51_002909"
     CDS             complement(<1..>487)
                     /locus_tag="IVK51_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAP6432406.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACCDF010000231          467 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCDF010000231 DACCDF010000000
VERSION     DACCDF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.241"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            1..467
                     /locus_tag="IVK51_002910"
                     /pseudo
     CDS             1..467
                     /locus_tag="IVK51_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          191 a           84 c           87 g          105 t
ORIGIN      
        1 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
       61 ttagctggaa agccacgtgt agaaatcatt cgagaatatg aactaacggc ttcagcattt
      121 gacaaatggg taaagcaatc taaaacgagt ggttcattca aagaaaaaga taatcttacg
      181 cctgaacaaa aagaattgtt agaactacgt aaaagaaacc agcaattaga aatggaaaat
      241 gatattttaa agcaagcagc gctgatattc ggacgaagag acaagtaatc gatgcgaata
      301 agcatcttta ccctatatca gcgatgtgca gaatattagg tctatcacgt cagtcctatt
      361 attatcaatc aaaaccaaag aaagacgaat cagaacttga agaagtagtc gctgaagaat
      421 ttatccgcag ccgaaaggcc tacggctcaa gaaaaataaa aaaagcc
//
LOCUS       DACCDF010000232          450 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCDF010000232 DACCDF010000000
VERSION     DACCDF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.242"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..430)
                     /locus_tag="IVK51_002911"
     CDS             complement(<1..430)
                     /locus_tag="IVK51_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP6432407.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          129 a           90 c           57 g          174 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt
//
LOCUS       DACCDF010000233          447 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCDF010000233 DACCDF010000000
VERSION     DACCDF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.243"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>447
                     /locus_tag="IVK51_002912"
     CDS             <1..>447
                     /locus_tag="IVK51_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432408.1"
                     /translation="HLEKAINTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKT
                     EYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYG
                     HHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMDATYIPLKRQT"
BASE COUNT          165 a           85 c           72 g          125 t
ORIGIN      
        1 gcacttagaa aaagcgataa acacactcct acggactgaa ttaacggctt ttttagatta
       61 cgaaaaatat gatcgcactg gttttaattc aggtaattcg agaaacggtt cttactttcg
      121 atcaatcaaa accgaatatg gtgaattaac attggaaata cctagagatc gtaatggtga
      181 gtttaaacaa caaactttac cagcctacaa aagaacaaac gatacattgg aaaccactat
      241 tatccattta ttcgaaaaag gtgttacgat gtctgaaatt gctgatttga tcgaaaaaat
      301 gtacggtcat cactatactc cacaaaccat gtccaacatg actaaagttc tgactgaaga
      361 agtaaatgcc tttaaatcca gagccttaaa tgataagtat gtcgctattt ttatggacgc
      421 tacttacatt ccactaaaac gtcaaac
//
LOCUS       DACCDF010000234          442 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCDF010000234 DACCDF010000000
VERSION     DACCDF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.244"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            46..>442
                     /locus_tag="IVK51_002913"
     CDS             46..>442
                     /locus_tag="IVK51_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP6432409.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEI"
BASE COUNT          138 a           83 c           86 g          135 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cg
//
LOCUS       DACCDF010000235          432 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCDF010000235 DACCDF010000000
VERSION     DACCDF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.245"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..417
                     /locus_tag="IVK51_002914"
     CDS             <1..417
                     /locus_tag="IVK51_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP6432410.1"
                     /translation="TSDDKPVIDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVDG
                     WHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPN
                     KKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          156 a           75 c           86 g          115 t
ORIGIN      
        1 acttcagatg ataaaccagt cattgatcca actagagcag gtgcagcaaa tcctacgctg
       61 acagatggaa caaatcacgc ccacattgat cagtttggag aaatcgaaaa cgcaaacttg
      121 catgtggatg gatggcacat tgctaattat aaatacgagt atattttcat tatggattac
      181 aatactggaa aagaattagc tcgagtaaga gctgatggaa tttatagacc agatgtaaat
      241 caagcttata atacttctgg aaacgttggt tatcatgtat ctttcaatat gcgtaatttt
      301 cctaataaga aagtctatgt aatgatgcgt gcaacgaatg atccagaagg gaacactaaa
      361 ggcggagcac aagattttca tgataaacgc tggtatttaa atattccgca acgataaaaa
      421 aagcccctcg at
//
LOCUS       DACCDF010000236          430 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCDF010000236 DACCDF010000000
VERSION     DACCDF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.246"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>430
                     /locus_tag="IVK51_002915"
     CDS             <1..>430
                     /locus_tag="IVK51_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP6432411.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTP"
BASE COUNT          143 a           84 c           73 g          130 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc
//
LOCUS       DACCDF010000237          396 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCDF010000237 DACCDF010000000
VERSION     DACCDF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.247"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..384
                     /locus_tag="IVK51_002916"
     CDS             <1..384
                     /locus_tag="IVK51_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432412.1"
                     /translation="RKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIE
                     NQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          152 a           71 c           60 g          113 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ctttaaggct gtttatcaag ctaactcaaa agaagaagcg
       61 aataccttct tatccggcat gattgagaaa tggaagaaaa actatcctaa agtgacgcag
      121 tcactcatag aaaaccaaga cttattaact ttttatgatt ttccacctag cattcgtaga
      181 accatttact caaccaatct aatcgagtct ttcaataagc aaattaaaag atacagccgt
      241 agaaaagagc agtttcaaaa tgaagaatca ctagaacgct ttctagtcag catttttgat
      301 acatacaatc aaaaatttct aaacagaagc cataaaggtt ttcaacaggt aaccgataca
      361 ttagtttcaa tgtttactga gtaactaatt attttg
//
LOCUS       DACCDF010000238          394 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCDF010000238 DACCDF010000000
VERSION     DACCDF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.248"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..285
                     /locus_tag="IVK51_002917"
     CDS             <1..285
                     /locus_tag="IVK51_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6432413.1"
                     /translation="EVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHM
                     LQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          117 a           73 c           92 g          112 t
ORIGIN      
        1 gaggttgcag aagatgatcc ttattgggaa atcgaagcct cctatattgc tcaatgtgcc
       61 tacaatacaa ccttgatgtt ttctccagat gtgatcattt ttggcggtgg tgtgatgaaa
      121 caacgccata tgctgcaaaa cgtccacgac gcatttgcaa aattggtcaa tgggtatgtc
      181 gaaacacctg agttaagcaa atatatcgtg accccagcat tagaagacaa cgctggaacg
      241 ctcggttgtt tggcattggc tcgagaagcg gtcttacatt cataaacgaa aaaaggctct
      301 tcgacattta gtgttggtag agaaagatac aactgaaaat ttagtcataa ccagttaggc
      361 agagtgctta actggttttt gttttacttg gaag
//
LOCUS       DACCDF010000239          374 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCDF010000239 DACCDF010000000
VERSION     DACCDF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.249"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            82..>374
                     /locus_tag="IVK51_002918"
     CDS             82..>374
                     /locus_tag="IVK51_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP6432414.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          133 a           62 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa ccca
//
LOCUS       DACCDF010000240          361 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCDF010000240 DACCDF010000000
VERSION     DACCDF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.250"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
BASE COUNT          123 a           39 c           58 g          141 t
ORIGIN      
        1 taggactcaa ttgttcatag atgagcattt gtaattaagt tatattataa taaaaaaata
       61 cgaattttag tgacgccatt atattgtttt tatttttcaa ttgtaatatt tcatattact
      121 tccataataa ggagtatttt ctattggaat atcttagctc ttttaaggat ttctcctttt
      181 ttgtttctca ttttttaaga ttatttggat ttcctttaca acaaacttcc acaagtatta
      241 ttgacataga tgtaattgag ggaagggggt tccagtgcta gaataaataa tgaaaagagg
      301 taacaaatgc tagcgttaag aggtgaacaa gtttgattga aagacatgta aatttattaa
      361 a
//
LOCUS       DACCDF010000241          341 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCDF010000241 DACCDF010000000
VERSION     DACCDF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.251"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>341
                     /locus_tag="IVK51_002919"
     CDS             <1..>341
                     /locus_tag="IVK51_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432415.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DACCDF010000242          324 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCDF010000242 DACCDF010000000
VERSION     DACCDF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.252"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>324
                     /locus_tag="IVK51_002920"
     CDS             <1..>324
                     /locus_tag="IVK51_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP6432416.1"
                     /translation="KQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFI
                     GTMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEG
                     FSHPEM"
BASE COUNT           84 a           67 c           85 g           88 t
ORIGIN      
        1 aaacaatacc aagcagattt agcagggatt gggattggct catttgggcc tatcgatatc
       61 catcgcgatt ccgccactta tgggtacatt acctcgaccc cgaaattggc atggcaaaat
      121 tttgatttca tcggtaccat gaaaaaagaa tttccgatcc cgatttcttg gacaacagat
      181 gtcaatgcgg ctgcctatgg tgagtatgtc ttcggcagtg gcaaaggatt gagcagtgtc
      241 gtttactata cgatcggtac gggtgttggc ggcggtgcct tacaagaagg gcgctttatc
      301 gaagggttta gtcatccaga aatg
//
LOCUS       DACCDF010000243          323 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCDF010000243 DACCDF010000000
VERSION     DACCDF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.253"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>323
                     /locus_tag="IVK51_002921"
     CDS             <1..>323
                     /locus_tag="IVK51_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432417.1"
                     /translation="FRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGSYDRT
                     VKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIADLIEK
                     MYGHH"
BASE COUNT           91 a           78 c           74 g           80 t
ORIGIN      
        1 ttccgtgtcc atttggaaaa agctgtcaat acgcttctca aaacggagtt aacggctttc
       61 ctcgattacg aaaagtacga tcgcattggt tttaacacgg gtaattctcg taacggctcc
      121 tatgaccgta cggtcaagac cgagtacggg gaacttcatc tccagattcc gcgcgaccgc
      181 aacggcgagt tcaagcaaca gactgttcct gcttatagac ggacgaatga taccttagag
      241 gagaccgtca ttcacctctt ccgaaaaggt attaccatgt cggaaatcgc agacttgatt
      301 gagaaaatgt atgggcatca cta
//
LOCUS       DACCDF010000244          322 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCDF010000244 DACCDF010000000
VERSION     DACCDF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.254"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(44..>322)
                     /locus_tag="IVK51_002922"
     CDS             complement(44..>322)
                     /locus_tag="IVK51_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432418.1"
                     /translation="GITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           87 a           81 c           60 g           94 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt cc
//
LOCUS       DACCDF010000245          307 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCDF010000245 DACCDF010000000
VERSION     DACCDF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.255"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            83..271
                     /locus_tag="IVK51_002923"
     CDS             83..271
                     /locus_tag="IVK51_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432419.1"
                     /translation="MLKKTMLVLGSISLGVLGFSPMLLSVIGKNNSKGELKLNPNYYN
                     SPNEPMFISEKQNDGQDK"
BASE COUNT          115 a           54 c           48 g           90 t
ORIGIN      
        1 aatttaaaca tttgacaaac tatagaacag cacttaaaat gtgtatacaa gtgacagcgc
       61 tttaaaaaaa gaggtgcagc ctatgttaaa aaaaactatg ttggtactag gttctatttc
      121 tctaggagta ttaggtttta gccccatgct attatccgta attggaaaaa ataattccaa
      181 aggggaactt aaattaaatc caaattacta caatagtcct aatgaaccta tgtttatttc
      241 agaaaaacaa aacgacggtc aggacaaata atcctgccgt cgttttgctc taacttctac
      301 cagtaca
//
LOCUS       DACCDF010000246          296 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCDF010000246 DACCDF010000000
VERSION     DACCDF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.256"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>296)
                     /locus_tag="IVK51_002924"
     CDS             complement(<1..>296)
                     /locus_tag="IVK51_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP6432420.1"
                     /translation="DWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSK
                     SAMYIADWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESG"
BASE COUNT           99 a           57 c           46 g           94 t
ORIGIN      
        1 tcaccgcttt ccaacatgaa aggaataaca ccagcaacat ataaatttgt atcaaattct
       61 ttcacaaaaa agtccatata ctcaagatat tgcatcgcat ttctcatcga ttttacttgg
      121 gtctgtaaga cgacgattgc ccagtccgca atgtacatcg cggatttact atatacagaa
      181 atagaaggtg ggacatctaa atagatgacg tcaaactcat caaataatgg ttctagcttt
      241 tctttcatat atttgaattg ggataatgaa tcattatatt ggtctttgat ccaatc
//
LOCUS       DACCDF010000247          291 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCDF010000247 DACCDF010000000
VERSION     DACCDF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.257"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            76..>291
                     /locus_tag="IVK51_002925"
     CDS             76..>291
                     /locus_tag="IVK51_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6432421.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIF"
BASE COUNT          111 a           55 c           50 g           75 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt t
//
LOCUS       DACCDF010000248          273 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCDF010000248 DACCDF010000000
VERSION     DACCDF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.258"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..>273
                     /locus_tag="IVK51_002926"
     CDS             <1..>273
                     /locus_tag="IVK51_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP6432422.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINH"
BASE COUNT          121 a           37 c           43 g           72 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cat
//
LOCUS       DACCDF010000249          268 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCDF010000249 DACCDF010000000
VERSION     DACCDF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.259"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            87..>268
                     /locus_tag="IVK51_002927"
     CDS             87..>268
                     /locus_tag="IVK51_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP6432423.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           55 c           43 g           86 t
ORIGIN      
        1 tttacctatg aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac
       61 tttttaacga ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa
      121 ctttggctgc cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata
      181 ccgttcaatc aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct
      241 tgatcaatgc cattgcagaa tcaaattc
//
LOCUS       DACCDF010000250          260 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCDF010000250 DACCDF010000000
VERSION     DACCDF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.260"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(<1..>260)
                     /locus_tag="IVK51_002928"
     CDS             complement(<1..>260)
                     /locus_tag="IVK51_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP6432424.1"
                     /translation="NQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGT
                     PSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNN"
BASE COUNT           85 a           46 c           41 g           88 t
ORIGIN      
        1 attatttcgt ttagatgcga cagattgaat aaaaaattca tctactgaat tgaaatccat
       61 agatttcaat aaaccatctc tacgtaacaa tccgttagaa ttctcgttta ggcctctttg
      121 agacggcgtt cctggatcag cgaaataaat ggcaatatca ttgacattac tgatctgttt
      181 ccaatttgaa aattcctttc cacaatcaaa agtgatggat ttgaataggt ttttcggtac
      241 agattcaaac cattgattta
//
LOCUS       DACCDF010000251          254 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCDF010000251 DACCDF010000000
VERSION     DACCDF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.261"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            46..>254
                     /locus_tag="IVK51_002929"
     CDS             46..>254
                     /locus_tag="IVK51_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432425.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNA"
BASE COUNT           96 a           36 c           62 g           60 t
ORIGIN      
        1 gcacacttgt tggtggtcat gcgggcggaa atgggggaat taaaaatgat tgattacaat
       61 gatcgtggac gtttaaagtg ggcagggttt tatttatctg atcatactga agaaattgat
      121 gccgacaatg aacaacgaac cagtcaaaat ttagcaaaag aacaaatgac gactgaagag
      181 attgcggaag tattgaacgc tgcagtcata aaaaatagat cagtatctat tcaaaaagaa
      241 gaacgaaacg ctga
//
LOCUS       DACCDF010000252          251 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCDF010000252 DACCDF010000000
VERSION     DACCDF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.262"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            47..>251
                     /locus_tag="IVK51_002930"
     CDS             47..>251
                     /locus_tag="IVK51_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP6432426.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQR"
BASE COUNT          100 a           42 c           41 g           68 t
ORIGIN      
        1 aaacgcatat agtagtctca aaactcccaa gaaatgagga ctacatatgc gttcatcaaa
       61 atatactgaa cattatacag atacttttat aacattcaag gaaattatga gaactgtttc
      121 taacatatac cacaattgtg taccagataa gattaaaaac cgaagaaata ctgatcaact
      181 gaagcaacgc gatacagtga ttattgcttg tgttatatgg ggcataatta atggctatac
      241 tagccaaaga g
//
LOCUS       DACCDF010000253          251 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCDF010000253 DACCDF010000000
VERSION     DACCDF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.denovo.263"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..159
                     /locus_tag="IVK51_002931"
     CDS             <1..159
                     /locus_tag="IVK51_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP6432427.1"
                     /translation="IKNHEKFLIRNYKISGGGCYLLECRFTSKKSMNSFLEQLNEYAN
                     YKLSIVID"
BASE COUNT          105 a           28 c           37 g           81 t
ORIGIN      
        1 attaaaaatc atgaaaaatt cttgattaga aattataaga ttagtggagg gggctgttat
       61 cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca gctttttgga acagcttaat
      121 gaatatgcga attataaatt atctattgtt atagattaat aataaaacta gcagatggta
      181 aaaatactaa tatgcgagac acacattcaa ccataactat ataacttatc aatttaaaac
      241 taaactgatg g
//
LOCUS       DACCDF010000254        23728 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.039, whole genome shotgun sequence.
ACCESSION   DACCDF010000254 DACCDF010000000
VERSION     DACCDF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.039"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            289..564
                     /locus_tag="IVK51_002932"
     CDS             289..564
                     /locus_tag="IVK51_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432428.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            577..2655
                     /locus_tag="IVK51_002933"
     CDS             577..2655
                     /locus_tag="IVK51_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP6432429.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2815..4701
                     /locus_tag="IVK51_002934"
     CDS             2815..4701
                     /locus_tag="IVK51_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP6432430.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4713..5660
                     /locus_tag="IVK51_002935"
     CDS             4713..5660
                     /locus_tag="IVK51_002935"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP6432431.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5680..6207
                     /locus_tag="IVK51_002936"
     CDS             5680..6207
                     /locus_tag="IVK51_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP6432432.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6270..6923)
                     /locus_tag="IVK51_002937"
     CDS             complement(6270..6923)
                     /locus_tag="IVK51_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP6432433.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7055..7897
                     /locus_tag="IVK51_002938"
     CDS             7055..7897
                     /locus_tag="IVK51_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP6432434.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8055..8951
                     /locus_tag="IVK51_002939"
     CDS             8055..8951
                     /locus_tag="IVK51_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP6432435.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8954..9667
                     /locus_tag="IVK51_002940"
     CDS             8954..9667
                     /locus_tag="IVK51_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP6432436.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9687..10205
                     /gene="msrA"
                     /locus_tag="IVK51_002941"
     CDS             9687..10205
                     /gene="msrA"
                     /locus_tag="IVK51_002941"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP6432437.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10202..10429
                     /locus_tag="IVK51_002942"
     CDS             10202..10429
                     /locus_tag="IVK51_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP6432438.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10581..11132
                     /gene="lepB"
                     /locus_tag="IVK51_002943"
     CDS             10581..11132
                     /gene="lepB"
                     /locus_tag="IVK51_002943"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP6432439.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11215..11757)
                     /locus_tag="IVK51_002944"
     CDS             complement(11215..11757)
                     /locus_tag="IVK51_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP6432440.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11994..12392)
                     /locus_tag="IVK51_002945"
     CDS             complement(11994..12392)
                     /locus_tag="IVK51_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP6432441.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12652..13512
                     /gene="ylqF"
                     /locus_tag="IVK51_002946"
     CDS             12652..13512
                     /gene="ylqF"
                     /locus_tag="IVK51_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP6432442.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13505..14272
                     /locus_tag="IVK51_002947"
     CDS             13505..14272
                     /locus_tag="IVK51_002947"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP6432443.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14328..15185
                     /gene="dprA"
                     /locus_tag="IVK51_002948"
     CDS             14328..15185
                     /gene="dprA"
                     /locus_tag="IVK51_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP6432444.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15307..17385
                     /gene="topA"
                     /locus_tag="IVK51_002949"
     CDS             15307..17385
                     /gene="topA"
                     /locus_tag="IVK51_002949"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP6432445.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17354..18730
                     /gene="trmFO"
                     /locus_tag="IVK51_002950"
     CDS             17354..18730
                     /gene="trmFO"
                     /locus_tag="IVK51_002950"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP6432446.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18928..19833
                     /gene="xerC"
                     /locus_tag="IVK51_002951"
     CDS             18928..19833
                     /gene="xerC"
                     /locus_tag="IVK51_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP6432447.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19870..20418
                     /gene="hslV"
                     /locus_tag="IVK51_002952"
     CDS             19870..20418
                     /gene="hslV"
                     /locus_tag="IVK51_002952"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP6432448.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20432..21832
                     /gene="hslU"
                     /locus_tag="IVK51_002953"
     CDS             20432..21832
                     /gene="hslU"
                     /locus_tag="IVK51_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP6432449.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21850..22641
                     /gene="codY"
                     /locus_tag="IVK51_002954"
     CDS             21850..22641
                     /gene="codY"
                     /locus_tag="IVK51_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP6432450.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22723..23598
                     /locus_tag="IVK51_002955"
     CDS             22723..23598
                     /locus_tag="IVK51_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP6432451.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
BASE COUNT         8212 a         4091 c         4999 g         6426 t
ORIGIN      
        1 tgattcatca gtcccataaa ttatagagcc gatttttgtc gcagccccat ttttttgttg
       61 tgataaaaga acaagaaagg caggaatggg aaagtaaaca tccttttgtc agaaactttt
      121 atctttctta agttttactt gatatctgat actatttgga atagaatgag gcgttgaaaa
      181 atttatggaa atttctaact agcaataaac gatgaaacaa tgaaatattt ttcatccaaa
      241 aagaactttg ctataatgat ggaagtttgt gtataggaga tctggaaaat gagagagagt
      301 tttgaacaac aaaaaaaact gttgcatgat cgctatggcg ccctttcgat ggatgaccgc
      361 cgtcaaatac tttgtaagtt gcgtaaaaga aatatattga tgtatcgtca gttggaacgt
      421 ctgaaacatg atctgcttcg tttggagtca aaacgtgtac agtgtgaatt agaggggaac
      481 caaacacagg ttgaagtagt cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa
      541 atgcttacac aaaataaaaa ataaagggga tggacgatgg aatttgctga gttagttatt
      601 gtatttgcag taacgatcac cttatcgaac atagctagcc gcattttgcc aatgattcca
      661 gctccactga tccaaatttt ccttggagtg gtattaggtc tgacagagtg gggacaatca
      721 atcgattttg aacctgaatt gttcttagtt atgattattg caccattgtt atttagagaa
      781 ggagaaaaag cagatatttc ttcgattctc aaaaatttcg ggacaatttt gttcttggca
      841 tttggaggag tcattttgac ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca
      901 agtgtcccac ttgctgcctg ctttgctttt ggagcagcgt taggtccgac agatgcggta
      961 gcagtcagtt ctctttctgg acgagtgaat atccctaaaa aggcgatgca tattttggaa
     1021 ggagaaggtc ttttgaatga tgcatctggt gttactgcct tccagtttgc tcttggtgcg
     1081 ctgatcaccg gatctttttc agcagtaaac gcggggatga gtctagtagt ttctagtatt
     1141 gggggagctt tgatcggctt tttgcttgtc tggttcaaac aaaaaataat tcatttgatc
     1201 gaaaaagcat cagcacaaga tgtcacaggg tatctgctga tcgaactttt gcttcctttt
     1261 ctagcctatg tattggcaga ggcatttgat gtatctggaa tcattgctgc agtggcagca
     1321 ggtattctcc aagcttctgg ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc
     1381 ttgtcccaca gtacatggac aactattgct tttacactga atgcattagt atttatattt
     1441 ttaggaatcg aattgacgca agtgttctcg ccagtatggg gagacggttt atatccaaac
     1501 ggtcttttat tagcgattat cgtattaatc tctgtcatgc tttttgtgat cagattcatt
     1561 tctatcagtc ttttttatgt attcaaagat ggcagtaaaa agttcaaaaa acagttgaac
     1621 gagatcttga tcttaacatt tggcggtgta aagggaacag tcagtttggc tacgattttt
     1681 attttgccat tttcaatcaa taatatgatg ttttaccagc ggtctttgct tttgtttctg
     1741 acggcgggtg tcatcttagt gactttggtc atagggatca ttgtcttgcc tatgctgacg
     1801 gaaacagagg aagcgaaaag cacggatttg aatgctttga tgattttaga agaggtcgtt
     1861 gaagttttgc gaaaagaaat aaaggaaatt gatcgtaata caaaagaatt tttagccact
     1921 gaagcagtga tagaaaatta tcaagaacgg atacgtgatc tgtacttgga agatttaacc
     1981 gatgatgaaa agcaagaagt acaagaaata caagcactga ttttgtcgat cgaacgggat
     2041 ggattggatg aaagttatcg ttcgggcaag ctttcttcta atggttatcg tttttactcc
     2101 cgttttcttt ctcgattcga acattctatc acaagccaga ttttgtcttt tatcggcttt
     2161 tggttcattg ttgtccgtag gttaatgcga atcgttctcc atccgaaaat gttttggcaa
     2221 agaagatatg ctgatcgtca gacatttatt agtgatgaag atatccaaga aatcagagag
     2281 acatatcaaa aaaatactca gttgattatt gaaagtttag ataacttgac tgatatttat
     2341 gatgaaacga tggttcaatt ttttatccag caacgcaaac ttgaaggaat aaaaatgaac
     2401 tcggggaatt tgatttcttc gtggatgatc cagcaagata gtctgtttac gaaaaaaatg
     2461 ctaagaggtt attatcttga aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc
     2521 acgacgtttt ctgcgaacag ctatcgcagg aatatcaatt tgttagaatc ttatacgatg
     2581 aacaaaccat ctgataactt ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg
     2641 agtccaataa aataaaaagg taaaaatttt caagttttat aaaaaacaaa acatatcaga
     2701 acaggatcga atcggtcctg ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa
     2761 attttcctga agttcactat ttttatgata caatatccaa gataaggagg gaacatgttg
     2821 aaagaattaa aagtaactga cctcaaaaaa acttatggtg aaaaagattt gtttgaccag
     2881 atttcttttt tggtccacga aaaagaccgt atcgggttga ttgggacgaa cgggacgggt
     2941 aaaacaagtc tcttaaatat tattgccggt atcgatagca gcgacggtga tcggcaaaca
     3001 gtgttttatc caactgatta ccgtattggt tatctttctc aagcagctga attttccgat
     3061 gaattaactg ttctccaagc agtctttcaa ggaaacagcc ccttgatcca aactgttcga
     3121 gcatatgagg aagcattgat cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa
     3181 cgttatgcta aagcagaaga acaaatgaat aaagaagatg cttggactac agataccaac
     3241 gcaaagatta ttttgcaaaa attaggaatc agtgaattag ataaaaaaat cagtacttta
     3301 tcaggtggtc agaaaaaacg agttagtctg gcgcaagtgt tgattgatga acccgattta
     3361 ctattactag acgaaccaac aaaccattta gattatgaag cgatcgagtg gttggaaaac
     3421 tatttgaaac aataccgtgg cgcactattg atggttaccc acgatcgtta ttttctagat
     3481 agagtagcaa accgaatctt cgaactttca tttggaaaat tatacgagta taaaggaaat
     3541 tatgaagctt acgtcttaga aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa
     3601 aagcgcaagc ggctttataa acaggaatta gcttggatga gggctggcgt acaggcacgt
     3661 ggcaccaagc agcaagcacg gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa
     3721 gtaaaccaag aagatgattt agagctgaac ctagcgacac aacgtttagg gaaaaaagta
     3781 ctggaaatca aagatggcag ttaccgtata aatgaccaaa cactgctaga acatttagat
     3841 ttattgattc aatctcggga aagactaggt attactggta aaaatggtgc gggaaaatcg
     3901 acattgctta atattctagc tggcagaatt cccctggata gcggtacaat gtctatcggt
     3961 gaaacggttc atcttgcgta ttatacacaa gaaaatgaag aaatggcacc agataaacgg
     4021 atgattgctt acttacaaga agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc
     4081 ggagtagcag aattacttga acgattttta tttccaagat ttatgcatgg gacactgata
     4141 ggcaaacttt caggcggaga aaaacgccgg ttatttttgt tgaaactgct gattcagcaa
     4201 cctaatgttt tattattgga tgaaccgacc aatgatttgg acatagcaac attgacgatt
     4261 ttggaagatt attttagaag ttttccaggt gctgtgatca ctgtttccca tgaccgttat
     4321 ttcttggaca aggtagcaga taagttatta gtattccaag gaaacggaaa acaagaactt
     4381 tattacggaa acatgtccag ttatttactg aagcaaaaag agacccagca gcctgctgaa
     4441 aaagcgaaac caaaaataca gagcaaggaa cctgctgaaa agaagaaatt atcttacatg
     4501 gaacaaaaag aatgggaaac gattgaagat gagattgcag aacttgaaga aaaaatctca
     4561 ttgctccaag aagagatgaa tcatcaagga gataatttca ctcgattaca agaattgcaa
     4621 aacgacgtat ctgaaacaga agcacaactt gaagaaaaaa tggcacggtg ggaatacttg
     4681 agtgaatggg tagaagatta ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa
     4741 gaaaattgtt ggaagaaggg catcgtaaag aagacagaac aggcacaggc acattaagct
     4801 tattcggata ccaaatgcgg tttgatctgc aaaaagggtt tcctttgtta acaacgaaac
     4861 gagtaccttt tggattgatc aaaagcgaat tgctttggtt tttaaaagga gatacgaata
     4921 tccgctatct attacaaaat aaaaaccata tttgggatga atgggcattt gaacgctaca
     4981 tcaaaagtcc agactataat ggaccagata tgactgattt tggccataga tgtttgacag
     5041 atcctgaatt caatgaagaa tataaaaagc agagcaagct tttttgcgag aagatcctaa
     5101 ccgatgattc ctttgctgaa acatacggcg atcttggaca catctacggc tatcaatggc
     5161 gtcactggga aacaaaagac ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa
     5221 tcaaaaagac tcctgattct agaagattga tcgtttctgc atggaatccg gaagatgttc
     5281 ctagcatggc tttgcctcct tgtcatacga tgtttcaatt ttatgttcaa gagggacggc
     5341 taagctgcca attgtatcaa cgcagtgggg atgtgtttct aggcgttccg ttcaacattg
     5401 caagttatgc gttgctgact cacctaattg cacatgaaac aggtttagcg gttggcgaat
     5461 ttgttcacac attaggagat gctcatcttt atatcaatca tatcgaacaa atgaaagaac
     5521 aattgagccg agaaatacgc gcatttccga ctttagtttt gaatcaggaa aagcagtcag
     5581 tatttgattt tgagatggaa gatatccaat tagaaggata tgacccacat ccggcgatca
     5641 aagctcctat cgctgtttag aatcagaaag gatgagaaaa tgttagctgc aatttgggca
     5701 caggatgaaa acggattgat tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat
     5761 ctgaaattct tcaaacaaat gacagaagca aatacactag tgatgggacg aaaaacattc
     5821 gaagggatgg gtagccgtcc gttgccgaat cgtcagacga ttgttttgac gagagattct
     5881 tcctataaag cagaaggcgt gcatgtcatg catagtgtgg aagacgtgct tgctctggaa
     5941 aaagaaacag acggtatctt tttcatcgct ggagggtcag ctgtttatca agaatttctg
     6001 ccattttgta cgatattata ccgtacagtc atccatcatg tattcgatgg agatgcttat
     6061 tttccacctg ttgactggtc tgattggtca ctgatcaata ctagtcaagg agaaatggat
     6121 gaaaaaaatc gttatccgca tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa
     6181 agcgggacag gcgaattgac gatataaaaa agctatggat agactagctg ttatttagag
     6241 ctgtcccatc catagcttta ttttttttat taagtaaaga ataaaacaga gaagtacata
     6301 aaacctgcac ctaacataac aaacaaatgc caaacaacat gcatatagcg gatagaattc
     6361 aagctataaa agaatgcgcc tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca
     6421 ccagtcattc caagagagtg ataaagagga acagcagcca cgacacagag ccaccccata
     6481 aaaatataga tgatcgtaga tacttttgat actctctccc gtttatgcaa cgtcaacgct
     6541 ttatatataa tgccaagaat cgcaaacaac cagattaata caaataaagt ccaacctagc
     6601 catcctcgga tactcagcaa acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc
     6661 gaactatgat cgaatacttg gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac
     6721 aaagtagacg ttaaaaataa aagaatcatc atcgatccat agatagcata agagacaata
     6781 tggagggagg aatgcaagtg ggcacctttt actaaaagaa ccactagtcc cgcgatactc
     6841 aatccagtcc caattccatg ggtgactgca ttaaacactt catttaatat ttcatacgtt
     6901 cgattaaatt tcgttttttc catgaaaacg gctcctttac taggtgtaat taatgaaaaa
     6961 tcattatttg tctgttatac taaaaataga aattgtctta catgtagttt aacatgaata
     7021 aggcatcttg ttaaagaaag agagagagaa aataatgaca aatgtaaaaa tcgtaacaga
     7081 ttcttcatgt acgatggaaa aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct
     7141 atccatcatg atcgacggaa cagtatatcc cgatgatgac caactagacg gcgaaagatt
     7201 tatggaaatg atggcgaaag caaaaaatct gcccaaaaca agccaaccac caattggaga
     7261 attcatctcg atgtatgatg agctaggaga agacggcagt gaagtgattt cgatccatat
     7321 gacaaaaggc ttgagtggaa cagtagaagc agcaagacaa gccagtaatc taacaaaaac
     7381 gaaagtaacg gttatcgaca gtgataccac tgaccaagga ttatcttttc aggtaattcg
     7441 agcagctgaa ttagcaaaag aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa
     7501 agtacaagac aatacaaaat tatacatcgg aatatccaca ctagataact tggtaaaagg
     7561 tggaagaatc agccgaacaa ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat
     7621 gaatttcgcc cattctgaat taatccctgt aactaagggg cgaggaaaga aaacgttcga
     7681 taaatggttt aataatttga aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg
     7741 tatttcacat gcggatgcaa aagaattggc gcaagaattc aaagagggtt tgcaagaatt
     7801 attcccggac atgcatatcc ctgtactgca tacgaatccg gttatcgcta cgcatactgg
     7861 taaagacgcg tttgcaatca tgtattatgt agattagtcg aaaagaggat aagccctcag
     7921 cctgaaaggc ttgcagctta ttctcttttt ctttataaaa ttaggaaaat aattgttttc
     7981 ttttttcata aagactacct ttttgataaa atttttgata acattagaac caatcgaata
     8041 caagaggtga aaaaatgaaa ttaacgaaac gacatttgat cctcgttttg ttcccattat
     8101 taattgcgat catcatctat gcaggtttag ctgcttctat accgaaagct ggcccactaa
     8161 tgaaaccagt aactactgtg agagaagatc caaataaaaa agaaaaactt cactacatag
     8221 ctatcggaga ttcgctgact gaaggagtag gagatcaaac caagcaagga ggattcgttc
     8281 cccttgtcgc aaatgatcta aaagaccgtt ataatttgac cgcggttgaa acagaaaact
     8341 atggtgtaaa tggtgaacga agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa
     8401 ttcgaaacaa tatcaaaaca gctgatttta tcacactgac tgttggcggt aatgatttga
     8461 tgaaagttat ccaaaataat ttattcgggt taacggtcaa ttcctttaaa aagccactga
     8521 aaaactatca ggaaaatgtc acagaactat tggaagaatt tcgatccctc aataaaaccg
     8581 cacctattta cattttaggc atctacaatc cattttattt gaactttcct gagatcactg
     8641 acatgcagac aattgttaac aattggaaca aaggaacaga aacggtgaca aaaaaaatgc
     8701 cgaactgcta ctttatccca atcaatgatt tgctttatca aggattgaat caggaagtag
     8761 gtgtcacaga agaaggaaca gatcaaacaa ctagcacgac agaaaatcta aatggcatca
     8821 aaaataatgt gctctatgaa gaagatcatt tccatccaaa taatttaggt tatcaattga
     8881 tggcaaatgc agtgagagat cagctgatcg agacacaaaa ccaatggtta gagaaggagg
     8941 atgatcaata aacatgaaaa caagatctga cgtatcttca aacaaaacaa agaaaacaaa
     9001 ttactggaaa ttcgccttcc ttatccttct tggggtagta cttggttcga ccgtattttt
     9061 aggcagccgt atttttgcta atcgggaacc agaactccca gaaataccag ctcttacgga
     9121 aagacaagga gatcctgtct taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat
     9181 cagctttttc cttagtgagt atcaaaaaga tagtgatatt gaatataaat tttatttgga
     9241 aaatgaagca ctactcaatg gtactttcga agtactaggt tttcctatcg atttctattt
     9301 atattttgat ccgtatgtga tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc
     9361 tatcggaaca ttgagtttgc caatcaaaga tgtgatgaat atgatcaaga gaaactataa
     9421 attaccagat tggatcgaag tcaatacaga tgatctgact atcatgctta ggttggatca
     9481 attccgaatg caaaacggga tgtatattaa agcagataaa atcgacttag taaatgatga
     9541 cattcggttt agtctatatt tgccaaaaga tacctctgat gcttcaaaca actcaaatac
     9601 caacaaaact agcaccagca gtactgaaag caagacaaaa gaaaataaaa aatcatcgga
     9661 tgagtagtat tagaggggga atcataatgg aacgagcaat atttgcagga ggctgcttct
     9721 ggtgtatgat ccagcctttc gacacgcttc ctggtatcca tacagtgatg tcaggatata
     9781 ctggagggca tgtaccagat ccgacttatg aacaagtaaa aagccaaacg actggacata
     9841 cagaagcagt ggagattatc tatgatccaa cattgatttc atatgaacaa ttagtagaac
     9901 tttactggca gcaaactgat ccaactgacg ctttcggaca attcgaggat cgcggtgaca
     9961 attatcgccc ggtgatcttt tatttgacag aagaacagcg gatcattgct gaaaaaagta
    10021 agaaacagtt agctgaaagt ggacgtttcg aacagccaat cgttacaaaa atcgaaccag
    10081 cccaaccatt ttatctagca gaagaagacc atcaagagta ttataaaaag catcctgaaa
    10141 attttaaaag aaaccatgca cgccgggctg cctttatcga agaaaattgg aggaacgaag
    10201 ggtgaatcga agcttttttc attatttaat gacactgcga ggcggtggca cacatgatgc
    10261 tttatctgtt ttcgctacag aagccggtaa agacatccaa tttcctaagc acagctctag
    10321 ctacgaagaa atttctaatt atctggaact gaatgtcgac tatttgccgt ccatggatat
    10381 ctttgacgaa gcatgggaaa aatatttaga aaataatcat gacatataag agatagtttt
    10441 gtactttatg atcaaaaaag ttttttgtcc aatagcctaa ttgaaagaat tatttttatt
    10501 tcagctatct agtcgcattt ttttgatatt tttcttataa taaagaaaga atgaaaaaaa
    10561 gagggatgga ttgatagaga ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg
    10621 tattcaaata cttacttgtt tctgtattcc ttgcgttcat gttgcgggga tttttattca
    10681 tcccggtacc agtggaaggc aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg
    10741 tgatggaaaa attttcggag atcagacgat ttgatattgt tgtttttcaa ttagcagatg
    10801 gcacgattta tatcaaacga gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg
    10861 atcaattgaa gatcaatggg aaagtagtta aggaacctta tctaactaaa aacctgaaat
    10921 cagatcatgc aaatgcatcc tatacgacgg actttacact acaagagcta acgggacaat
    10981 ccaagcttcc ggaagataat tattttgtac ttggagataa tcgaagagtt tcaaaagata
    11041 gtcgttcgtt tggcacgatc aataaaactg atattctagg taaagcaaga tttgtctatt
    11101 atccacttga tgaaataaaa tggatccagt gagaaacaat aaagcgtgca gtctgttgag
    11161 actgcacgct ttattgttta atccaaaatc agtttactac cttagtacgt aaaatcaaac
    11221 agctcgttta aacacttttc tagcctctaa agttttgata aatgaataaa caaccattat
    11281 ttgaataggg atttggatca tattcctgat gaatctacct agaaaaatta ctttgaaatc
    11341 tgcaccaaac aaaacagaca tcagccaaga attgataaaa aggctgactg ggatcataat
    11401 caataattgg gcagcgatga cggaaggcag gtttatcttt ttatgatata aaaataagcc
    11461 attaataaat ccaccaagga aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc
    11521 aaatatcgtg aaatttaaag tgttcgacaa catttcaagc aaaccagccc agatcggtcc
    11581 atatagataa cccataagcg ccatacttaa aaatccgaat gtgatttgaa aataagcagg
    11641 accaatcgct atcttgtata agatgacatc aagggctaat aaaagagcag aggtcgacaa
    11701 caatcttgca ttcatttcgg gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat
    11761 attctctcct ttgtcagtta ataataaaag gataccataa aatcaccaat gtgtcatgac
    11821 acattggtga tttacataca ctggcatcta acaattaaaa aaagggtgag aagactgctt
    11881 ttataaactg taaaaaagag attgtacgaa taaaaaaaat agaggcggag aatacttatc
    11941 gtatcatccg cctttgtttt tttgttataa aaaacgtcag attagttatc tgcttacatc
    12001 ttcatccata cgggttctaa tgtatttccg tctagatctt ggacttctaa tgaaaacatt
    12061 tcttcttcag gcattcccat atctacttcg tggaaagtac caccattttc ttttgcaatt
    12121 tgagcaaatt tttttacttc atccgcattt tcaacactaa acgaaatcaa agcaccactt
    12181 gtttttttat tatcagctat ttctttatct tttataaatt ttttataaaa atcatgagag
    12241 agaagcatta tccaaaaagt gtcatcccat accattgaac cagcttcttc agtagaaaat
    12301 tcttcatttt ttttaaaacc cagcttttca taaaaagcaa cggaacgatt gacatcagca
    12361 acaggaaaat taacaaaaac catactattc atttgtttcg tctcctttct gttttgttcg
    12421 tctatcatct cttatttagg aaacgctgtc aaaaatttag attcatatat agaaaccaaa
    12481 ataaaaattt gcatcatcaa aaaagtgaag atgtgataga atgaggactg gttcactttc
    12541 acttaactaa tagatggaat ttctttataa taaaaaagtg aatttatatt gaaaaagaaa
    12601 aaaacaagta aagccgggga atattccggt atgaaaggaa tggagagaac aatgaccatc
    12661 caatggtttc cgggacatat ggcaaaagcc cgacgagaag tttctgaaaa aatgaaatat
    12721 gtggacatcg tatttgaatt ggtagacgca cgattacctt tatcttctcg taatccaatg
    12781 ctagatcaga tcattcagca aaaacctcgc cttgttttat tgaataaagc ggatctggca
    12841 gatccacaac agacaaagct gtggcaagaa tattttcgta aacaaggcca tcttgcatta
    12901 gctatcacgg cacaagaaag taaaggaatt aaaggtctag tacctgcggc taaagaagca
    12961 ttgaaagaaa aacgggcaag agatgctgct cgggggatca agcctcgcgc tattcgggca
    13021 atggtcttag gtattccaaa tgttgggaaa tctacgttga tgaatcgact agtaggcaag
    13081 aaaattgcac agacaggaaa taagcctggc gtaacaaaag gtcagcagtg gcttcgttta
    13141 ggtaatgagt tggaattgtt agacacacca gggattttat ggcccaaatt tgaagatgaa
    13201 gaaatcggca aaaaactagc gctgacaggc gcgatcaaag accagcttgt ccatttagat
    13261 gatttagcga tttacggatt agactttttt gcccgatatt attcaggaag aatcagcgat
    13321 cgttatggtt tatcaatcga gcaagaagaa aaattggcac cggaattact gatggatatt
    13381 acagcaaaac gtgggtataa agaagactat gatcgtggca gcgaattagt cgtgcatgat
    13441 attcgtcaag gaaaacttgg acgatacaca ctggatcgtt gcgaagaaat ggagcaaaaa
    13501 agtgatgact gaatccatcc aaagtatcaa agaacgattg aaaagcatac agagaataga
    13561 tgatcctaca ctagaatcat taagaaatga tccacgcaaa ggcgtgcagc aagcactggc
    13621 ttcttttgaa cgacgattga aaaaggaagc attaattaag gaaaaatatg aagaaatgtt
    13681 ttgctttgaa aagcaagggt acgcacaagg tcaccaatgg attgccggca ttgatgaagt
    13741 aggaagagga ccattagctg gacctgtcgt ggcagctgca gttatcttgc cgaaaaatga
    13801 gatcatatta gggctgaatg actccaaaca attatcagag aaaaagcgag aacttcttta
    13861 tcaacaagtt caagaaaaag ccctggcaat cgggatcggg atcgttgatc agcagacaat
    13921 cgatgatgtg aatatttatg aagcaagtaa attagcgatg atccaagcag tagaaaaatt
    13981 gtccattgta ccaagctatt tgttgatcga tgcgatgacc ttggatttgc ctattcagca
    14041 ggaaaaaata atcaaagggg atgcacgatc tgtgtcgatt gctgctgcaa gtatcattgc
    14101 taaagtatat cgagaccaat tgatgaaaga atatgacgtg ctttatcctg gatatggttt
    14161 ctcgcataat gcaggttatg gaacaaagga acacttagaa ggacttgaac gacaaggaat
    14221 tacgccaatc catcgtaaaa gttttgcacc tgtaaaaaaa tatttacaat aaaaaacagc
    14281 cgcttttacg tatcttatta gaaagagaga cgaaaggcgg ttttatgatg tatcaaatag
    14341 aagaaaattt attgaaactt gcttatacaa aaggaatcag tagtcaagga aaatgggcac
    14401 ttgctaactg gatgatgcag tatccttata aagaaatcgg atttgaagac atcattaaga
    14461 taggtaaaat aaccaaacac cgggagctgt ttcgcaaaag ttggcaggaa attcacgcta
    14521 actggggcaa aatccaatcg aaacagtcgt ttatcacttg ctttgatccc aaatatcccc
    14581 ctcagctgct tcacttgacc tatccgccaa ttgtactgtt ttataatggt gatctatctt
    14641 tactttcttg taacatgtta agcgttgttg gtggaagaca cacttctgct ttagccaaaa
    14701 aaacagttta ttatttacta aaaccagtga tagaagctgg atatgtgatc gtcagtggct
    14761 gcgcgaaagg gatagataca tacggtcacc aagcagcaat ctctcatggt ggacggacaa
    14821 tcgcagtcat tggaacaggg atagagcaag tctatcctgc tgaaaatcgt gacttgcaag
    14881 aggtgattgc aaatgatcat cttcttttga gtgaatatcc tcctcaatcc ggcccgaaaa
    14941 agcattgttt tccaatgaga aatcggatta tcgcagcttt gtctaaagga acatgtgtct
    15001 ttgaagccaa gaaaaaaagc ggctctctga taacagcaca gcaagcattg gatcttggaa
    15061 gaacagtttt ttctgttcca ggaaatattt taacagaaca ttctaccgga tgtcatcatt
    15121 tgatccaaga cggtgcagtt tgcacggttt cgggtgaaga tatcttggca gaatttaaag
    15181 aataacacca tacttttcat ttgcttttct tgacaaacgt tttctgaatg gatatgatta
    15241 gctacgattt gtatgaatcc tcttttaata gtaggaacac gacaatggaa aggagtccat
    15301 tcagtaatgg cgtataaata tttagtgatt gtagaatcac ctgcaaaagc caagacaatt
    15361 gagaaatatc ttggacgaaa ctataaagta atggccagtg ttggccatat aagagattta
    15421 ccgaaaagta aaatgggtat cgattttgaa aacaattatg aaccccatta tatttctata
    15481 cgcggaaaag gcgatgtcat caaaagcctg aaagccgcag caaaaaaagc tcaaaaagtt
    15541 tacttggcaa gtgacccgga tagagaagga gaagcgattg cttggcattt agcgtaccta
    15601 cttgggttgg atctgaaaga aaaaaatcgg gtggtcttca atgaaatcac aaaagacgca
    15661 gtcaaagcag cttttaagga accaagaacg atcgatgtag atttagtaga tgcacagcaa
    15721 gctcgtcgta ccttagacag aatcgttggt tattcgatca gtcctattct ctggcgtaag
    15781 gtcaagaaag ggttaagtgc aggacgtgtc caatctgtcg ctttaaaaat tattattgac
    15841 cgtgaaaaag agatccgaga atttgttcca gaagaatatt ggagcatcga cggtaatttt
    15901 aaaaaagctc gcaagaaatt caaagcaaat ttctggggaa tcgacggtaa gaaaaagaaa
    15961 ttaccagatg cacaaagtgt aaaagaagtc actgctagaa ttgatggaac agacttcgaa
    16021 gttaaaaaag tagaaaagaa agagcgcaaa agaaaccctg cgaatccttt cactacaagt
    16081 agcctgcaac aagaagctgc ccgtaagtta aactttcgaa caagaaaaac catgatggta
    16141 gcacagcaat tatatgaagg aatcactttg ggtaaacaag gaacagtcgg cttgattaca
    16201 tacatgcgta ctgattctac ccggattgcc gatacagcaa aagcagaggc cgcagcattt
    16261 attgaagata cgtatggaaa agaattttct agacatgatc accgaaagat caaaaatgct
    16321 caaggcgcac aagatgccca tgaagctgtg cgtccaacga gtgtgcttcg tacaccagat
    16381 gaactcaaac aatatttaga taaagatcaa ttgaaacttt atacattgat ctggtcacgt
    16441 tttgtggcta gtcagatgac accagctatt ttagatacaa tgaaagtgac attagaacaa
    16501 aatggtgtca tcttcattgc gaacggttca aaaatcaaat tcaaaggatt catgcaagtg
    16561 tatgtggaag gtagagatga cggcaaagaa gagaaagaaa atattcttcc agaacttgaa
    16621 gaaggcgatg tagtccaatc tgttgatatt gaaccaaaac aacattttac tcaaccacca
    16681 gcacgtttta gtgaggctac gttgattcgt gcactagaag aaaacggggt agggcgacct
    16741 tccacttatg caccaacact tgatacgatc caaagacgct actatgtaaa attgacacaa
    16801 aaaagatttg agccgactga actaggagaa attgtcaatt cattaatctg tgaattcttc
    16861 cctcaaatcg tcgatatcca tttcacagca gaaatggaag gagatttgga taaaattgaa
    16921 gaaggcacgg aagcgtgggt caaagtggtt gatcgtttct ataaaccgtt tgaaaaagaa
    16981 ctgacgaatg cggaagaaaa aatcgaaaaa attcaaatca aagatgaacc agcaggattc
    17041 gattgtgatg tctgtggaca tccgatggtg ataaaattag ggaaatatgg gaagttttat
    17101 gcttgcagta attttcctga ttgtcggaat acgaaaccaa tcgtaaaaga aatcggtgtg
    17161 acttgcccag tatgtcatga aggacaagtc attgaacgaa aatctaagaa aaacagacta
    17221 ttctatggtt gttcccgttt cccagaatgt gaattcactt cttgggataa accagtgggc
    17281 agaaattgtc caaaatgtga cggatatctg gtcgagaaga aagtcaaagg cggtaaacaa
    17341 gtagtctgtg taaatggaga ttacgaagaa gacatccaaa aataatagaa aagaggttct
    17401 ttcattgaaa atggtaaatg ttgttggagc agggcttgcc ggaagtgaag ctgcttggca
    17461 gatcgcacaa gcaggagtcc ctgtccgttt atatgaaatg cgtccagtca aatcgactga
    17521 ggcgcatcac acaagtaatt ttgctgaact agtatgttcc aattcattaa gaggaaacag
    17581 tttggctaat gcagtagggt tattgaaaga agaaatgcgc cgattgaatt cggtagtaat
    17641 cacttcggca gatgaaacag cagtgccagc tggtggtgcc ttagctgttg accgcgattc
    17701 tttttctgaa acaattaccc gaaaagtcaa agagcatcca ttagtaacag tgataaacga
    17761 agaaattact gaaataccgg aaggaatcac cgtagtagca acaggacctt tgacttctca
    17821 tccattagca gaaagcatca aagcattcaa tggttcagat ggtttttatt tctatgatgc
    17881 agctgcacca atcgtagaca aagctacgat cgatatggat aaagtatatt tgaagtcccg
    17941 ctatgacaaa ggagaagcag cttacttgaa ctgtccgatg acagaagagg aattcaaagc
    18001 tttccatgag gcactaatca atgccgaagt ggcagaactt aaatcttttg agaaagaaaa
    18061 atattttgaa ggatgtatgc caattgaagt aatggcacag cgaggattca aaacgatgct
    18121 tttcggacct atgaaaccag ttggattgga agatcctaaa actggcaaac gtccttatgc
    18181 tgttatccag ctgcgtcaag acaatgctgc agcttcttta tacaacatcg tgggctttca
    18241 gactcacttg aaatggggcg agcaaaaacg tgttttccgg atgattcctg ggttagaaaa
    18301 tgctgaattc gttcgctatg gtgtgatgca ccgaaacagc tttatgaatt cgccagaatt
    18361 gctgaaaccg acttaccaat caacgaaacg cgaagatctg ttctttgcag gtcagatgac
    18421 tggtgtagaa ggctatgttg aaagtgcagc gagcggacta cttgctggaa tcaacgccgc
    18481 acgcttagca aaagaaatgg aaccagtcga gtttccacaa gaaacagcta ttggaagtat
    18541 ggcttactac attactcatg cggaaggaaa acatttccaa ccgatgaatg ccaactatgg
    18601 tctgtttcct gaactgccag agcgcattcg agacaaaaaa gcaagatatg aagcaattgc
    18661 acaacgagct ttaaaagcta atgaggcaat aaaaacagaa ttagaaaaaa ctgcaatccc
    18721 aaatgagtaa ggaaactcca tgaagagggt gagacggata gtctcgctct ttttcttgcg
    18781 tttttcttaa gaatttctca aacaaaatgt gaggaattgc gccaattgtg aacaaactta
    18841 gaaatattgg ttaaattctg acaatttatt tgtcttttta taaacgctgt gctaaattat
    18901 tctctgggac aaaaggaagg tgagaaaatg acagaagcca cgtgggaaca aaaatttttg
    18961 cgttatttga ttgtcgaaag aggatattca gaaaaaacga aagaagcata tgaagaagac
    19021 attctccatt tcaaacattt tttggaggaa tctggagacg ctgatctgct gaaggcggaa
    19081 tattttacgg ttcgtgtcta tttgagtgca ttagctgatc atcgtcctag ctatagcaga
    19141 aattctatca gtcgcaagat agccagccta cgttcgtttt atcagtatct tctcaaagaa
    19201 gaagtgataa aagaaaatcc attttcttac gttcatctca aaaagaaaaa tttgagactg
    19261 ccacgttttt tttatgaaaa tgaaatgcaa gcactttttg acagcgtagc aggagatagt
    19321 cctttggatt tgaggaaccg cgctttactg gaagtccttt acgggtcagg catccgttta
    19381 agtgagtgta gcagtttgtc ggtatcagac attgattttg attcagaggt catgttgatc
    19441 catggtaaag ggaacaaaga gagatatgct cctctaggtt cttttgctca agatgctctg
    19501 caagaatatt tcagggaagg ccgccaggta ttgatgacaa aatatcacga agagcatgat
    19561 atcgtttttg tgaatcatca tggaaaacca atcacgccta ctggaattga atatgtcttg
    19621 aatcaaatga tcaaaaaaag cagtttggat agtgatatcc atcctcacat gctgcgccat
    19681 acatttgcta ctcatttatt aaataatggg gcagatttgc ggacagtcca agaattgcta
    19741 ggacatgcca atttatcgac tacccagatc tatgcccatg taacaaaaga aagtttacaa
    19801 aaaaactatc gttctttcca tccaagagcg taaaaataag gaaaagaaca aatttaggag
    19861 gaaacaaaca tggtcgaatc accatttcat tcaaccacta tctgtgccgt cgaaaaagac
    19921 gggaaatttg ctatggctgg agatggccaa gtaacaatgg gtgaatctgt tgtcatgaaa
    19981 ggcactgcaa agaaagtacg acgtatctac aatggagaag ttgttgtagg tttcgccgga
    20041 agtgttgcag atgcgtttac tttagaagaa aaatttgaag gcaaactaaa cgaatataac
    20101 gggaatctac aacgcgcagc tgtcgaatta gctcaagaat ggcggacaca gcaatctatg
    20161 caaaaacttg aagcgatgct gatcgtaatg aatgataaag aaatgcttct tgtttcaggt
    20221 acaggagaag ttatcacacc agatgatggt atcttagcta tcggatcagg agggaatttt
    20281 gcgttggctg cagcccgagc aatgaaaaac tatgctcaaa acgacatgag tgcgaaagaa
    20341 attgcagaaa atgcgttaaa tattgccgca gatatctgta tctttactaa tcataatatt
    20401 atcgtagaag aactgtaaga ggtgagtaga tatgtatgaa ttaaaacaaa caccaagaca
    20461 aatcgtaagt gaattagatg aatatatcgt aggacagcac gcagcaaaaa aatctgttgc
    20521 cgttgctttg cgtaaccgct atcgccgttt gcagttagat gaaaaaatgc aacaagatat
    20581 tacaccaaaa aatatgctga tgatcggacc tacaggggtc ggtaaaacag aaatcgcacg
    20641 acgtttggca aaaatcgtca atgcaccatt tgtcaaagtc gaagcaacaa aatttactga
    20701 agtcggttat gtcggaagag atgtagaatc aatggttcgg gatttagtag aaaatgcgat
    20761 ccaaatcgta gaaaaagagc agtacagccg tgtatacaca agtgctaaaa aaaatgcaga
    20821 aaaacgtttg gtaaaaatct tggtacctgg tatcaaaaaa gaacagaaac aatccactaa
    20881 tcaatttgaa caaatgatga atctttttaa tgctagtcag caaaataccg ctgaaccgca
    20941 agaagaagtg acagaagata ttcgaatcaa tcgccgtaca gtcttggaac aactacaaaa
    21001 gggtttactg gatagtcgtg aagtaaccat tgaagttgaa gaccaaaaga aacctgcacc
    21061 aatgatgaac aatggattgg agcaaatggg gatcgaccta aatgaaactc taggtgcttt
    21121 gacaccgaag aaaaaagtgg aacggacggt aacagtcaaa gaagctttag agttattgat
    21181 caaggaagaa tcagcaaaat tagtcaagga tgctgatatc catagtgagg caatcaaatt
    21241 agcggaagaa agtggaatta tttttatcga tgaaatcgat aagatcactt ctaaatcaca
    21301 gcaatctgga gaagtatcca gagaaggcgt ccaaagagac atattgccaa ttgtcgaagg
    21361 ttcacaagta aacacaaaat acgggatcat ccaaacagat cacgtcctat tcattgcttc
    21421 aggagctttc catttgtcta aaccaagtga tctaattcca gaacttcaag gacgcttccc
    21481 gatacgggta gaattagatg atttgacagc tgaagatttt gtgaagattc tgaccgaacc
    21541 aaataatgct ttagtaaaac aatacgtcgc gttgatcggt acagaaaatg tgtcagttac
    21601 gtttacaaaa gaagcaattg aacggatcgc tcaaatcgct ttcgatgtca accgtgacac
    21661 agataatatc ggcgcacgca gactgcatac cattttggaa aaactactag aagatctatt
    21721 atttgaagca ccagatatgc aaatgggaga aataacgatc acagaagcat atgtggatga
    21781 caaacttggc tcaattgcgg caaatgaaga tttaagtcgc tatattttat aaaaaatcgg
    21841 aggacgacaa tgacagactt gctaacgaaa acccgcaaga taaacacact tttgcagcaa
    21901 aagaatactt ttgacatgca agcagatttg ccttacgata aaatggctgt cacactaggt
    21961 ggtattcttg acagcaacac ctatatcata ggaagtgacg gagtattact aggttataat
    22021 gaacgtcacg atgtgaacaa tgaacgaatc aaaacaatgt ttgttgagaa acaatttcct
    22081 gaatcttata ctgatacagt ggcacagttg atgaaaactg aagctaatat cccaattact
    22141 agtgatatta ctgcatttcc ggtagagtca agagagaaat atccatttgg tctgacgact
    22201 gtcgttccga tctttggagc tggcgaacgt ttgggtacga ttatcttatc tcgtatggaa
    22261 caatcgtttg cagatgacga tctagtctta gcagaatatg gagctacggt tgtcggtatg
    22321 cagatccttt atcagaagtc tcgaagcatc gaagcagatg tacggagtgc aacggctgtc
    22381 caaatggcaa tcaacacatt atcttatagc gaattgaagg ctgtacaagc tatctttgaa
    22441 gcattggatg gagatgaagg tcgtctgact gcttctaata ttgccgatag tatcggcatc
    22501 actcgttctg tgatcgtcaa tgctttacga aaattggaat cagctggaat cattgaatct
    22561 cgttcgttag gcatgaaggg aacttattta aaagtattga attcccgttt taaagacgag
    22621 ttagcaaaac acagttatta gaagtaaggg gactggggca gatagaaatg tttgttccag
    22681 ccttcccatt ttaaaaagta agtcgaagtt ggaggaatgg caatgactgt tattattcaa
    22741 aatgaccagc tgatcgccga aatcgctgaa cacggtgcgg aattgatcag tctgaaatca
    22801 aaagaacatg gtttagagta tatctggcaa ggtgatccag cgtattgggg cagacatgca
    22861 ccggttttat tcccttttgt tggacgttta aaagatgatc agtatgttta tcaagggaaa
    22921 acatatcaaa tggggcaaca cggatttgct cgggatatgg attttgaagt cattgaacat
    22981 ggacaagaaa tggctagttt catcttaagg agtacgccgg agacattaga aaagtatcca
    23041 tttgattttg aattagtgat cagctatgaa ttaggaggag acggcatcac ggttcattat
    23101 caagtggcaa ataccggtga aaaagaaatg ttcttttcta tcggtggaca tcctgctttc
    23161 aatgtacctc ttgaagaagg attgtcattt gaagatttct atctggcatt ttctccattg
    23221 aaatcaagat tacgcctacc attgaaaggc ccattcatcg atatggatca aaagacgtta
    23281 ggtcagacaa atacgaatct ggctttgaat cgtgaacttt ttgagcaaga tgccttggtt
    23341 tttgaaacaa ggggtctaaa tgcgtacgcc attcgaagtg aaaaatcttc ccatagcgta
    23401 actttaagct ataaagatat gccttatgta gggatctggt ctccttatcc aaaagaagcg
    23461 cctttcgttt gtatcgaacc gtggtgcgga atcgctgaca cggtgaacag tagtggaaac
    23521 ttgatggaaa aagtagggat caatcaacta ggtgcccatg aaatcttcaa gacaaaatat
    23581 tccatcacag tgaagtaaga ggttgtgacc gagaagttag cagggaacac cgtttaccaa
    23641 ggtgtgtaaa aaagctatcc tgtaagtaat aagggagtgt gacatcgatt tgtggctctt
    23701 tgtcaataag gactgatgag ttgtgcaa
//
LOCUS       DACCDF010000255        20985 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.046, whole genome shotgun sequence.
ACCESSION   DACCDF010000255 DACCDF010000000
VERSION     DACCDF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.046"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(134..577)
                     /locus_tag="IVK51_002956"
     CDS             complement(134..577)
                     /locus_tag="IVK51_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP6432452.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IVK51_002957"
     CDS             complement(795..2135)
                     /locus_tag="IVK51_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6432453.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IVK51_002958"
     CDS             2404..3300
                     /locus_tag="IVK51_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP6432454.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IVK51_002959"
     CDS             complement(3450..4391)
                     /locus_tag="IVK51_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP6432455.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IVK51_002960"
     CDS             4517..5638
                     /locus_tag="IVK51_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP6432456.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRKNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IVK51_002961"
     CDS             5896..7398
                     /locus_tag="IVK51_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP6432457.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IVK51_002962"
     CDS             7465..7992
                     /locus_tag="IVK51_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP6432458.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IVK51_002963"
     CDS             8203..9264
                     /locus_tag="IVK51_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP6432459.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IVK51_002964"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IVK51_002964"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP6432460.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IVK51_002965"
     CDS             10177..12159
                     /locus_tag="IVK51_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6432461.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVK51_002966"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IVK51_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP6432462.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IVK51_002967"
     CDS             12881..14305
                     /locus_tag="IVK51_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP6432463.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IVK51_002968"
     CDS             complement(14486..15259)
                     /locus_tag="IVK51_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP6432464.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IVK51_002969"
     CDS             complement(15256..16161)
                     /locus_tag="IVK51_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP6432465.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IVK51_002970"
     CDS             complement(16315..16635)
                     /locus_tag="IVK51_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP6432466.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IVK51_002971"
     CDS             17109..17795
                     /locus_tag="IVK51_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP6432467.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IVK51_002972"
     CDS             17797..18741
                     /locus_tag="IVK51_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP6432468.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IVK51_002973"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IVK51_002973"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP6432469.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVSFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IVK51_002974"
     CDS             19663..20940
                     /locus_tag="IVK51_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432470.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6779 a         3783 c         4361 g         6062 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaaaaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgtca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACCDF010000256        20863 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.047, whole genome shotgun sequence.
ACCESSION   DACCDF010000256 DACCDF010000000
VERSION     DACCDF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.047"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            267..1304
                     /locus_tag="IVK51_002975"
     CDS             267..1304
                     /locus_tag="IVK51_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6432471.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IVK51_002976"
     CDS             complement(1393..1716)
                     /locus_tag="IVK51_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP6432472.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IVK51_002977"
     CDS             complement(1703..2410)
                     /locus_tag="IVK51_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP6432473.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IVK51_002978"
     CDS             2496..2837
                     /locus_tag="IVK51_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP6432474.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IVK51_002979"
     CDS             complement(3008..3856)
                     /locus_tag="IVK51_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP6432475.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IVK51_002980"
     CDS             4454..5317
                     /locus_tag="IVK51_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6432476.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IVK51_002981"
     CDS             5417..5899
                     /locus_tag="IVK51_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP6432477.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IVK51_002982"
     CDS             complement(6162..6629)
                     /locus_tag="IVK51_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP6432478.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IVK51_002983"
     CDS             6752..7603
                     /locus_tag="IVK51_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6432479.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISILAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IVK51_002984"
     CDS             complement(7854..9812)
                     /locus_tag="IVK51_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP6432480.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IVK51_002985"
     CDS             complement(9809..10681)
                     /locus_tag="IVK51_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP6432481.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IVK51_002986"
     CDS             complement(11023..11895)
                     /locus_tag="IVK51_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP6432482.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IVK51_002987"
     CDS             12023..12592
                     /locus_tag="IVK51_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP6432483.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IVK51_002988"
     CDS             12659..13396
                     /locus_tag="IVK51_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP6432484.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IVK51_002989"
     CDS             13627..14895
                     /locus_tag="IVK51_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP6432485.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IVK51_002990"
     CDS             15382..15846
                     /locus_tag="IVK51_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP6432486.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IVK51_002991"
     CDS             15830..16459
                     /locus_tag="IVK51_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP6432487.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IVK51_002992"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IVK51_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP6432488.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IVK51_002993"
     CDS             18338..18784
                     /locus_tag="IVK51_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP6432489.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IVK51_002994"
     CDS             complement(18983..19498)
                     /locus_tag="IVK51_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP6432490.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20817
                     /locus_tag="IVK51_002995"
     CDS             19615..20817
                     /locus_tag="IVK51_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP6432491.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         6632 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt gtggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtata ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaac attgatgaca ggattgttta tcatcgttgt attcgctggt
    19921 cttggcggat tatctgggaa tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc
    19981 gggaatgctt tatttgtctc tactgcttta gcaacgatcg tcagtatttt agttggtaac
    20041 acagaaaaag caatcatgat gtatgaagca gcacttggct gcggaatggc tgttggccct
    20101 ctagtcggtg gggtcttggg cagtggttca tggcgttatc cattttttgg ggtagcattt
    20161 ttgatgtttt gcggttttat agccctagcg gtacttttac ctgaaactga gaaaccaaag
    20221 agaaaagtag gcctttggga aggtgtcaaa gcgttaagca atcataaact tcgttcagtt
    20281 ggactaatcg cattgttata taatttcgga ttctttactt tacttgcgta tgcgccattt
    20341 ttattgattg gctattctga attagaagtc ggttttgtat tttttggctg gggtgtgctt
    20401 ctggctattg cttcgatttt cgttgcaccg gctcttgagc ggaagtttac gacttatcgg
    20461 acaattttag cagcatttgt cttattcatt atttgtttgc tgttattagg agctggaact
    20521 tctatgccag tccttgtacc gatcagcatt gtgttagcag gatttttcca aggaattatc
    20581 aatacactgt tgacgacgat tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg
    20641 tccagctata gttttgtccg ctttttcggt ggagctttag caccatttat tgcaggtgtg
    20701 attggtgaac atattggtga aagctatgcg ttttattttg caggtgccat tgttcttatc
    20761 agtgtatgtt gtatcattta tcaccgtaat tattttgcta caaatgaaga agaataagaa
    20821 atcagaggtt gtgaaaaagc tgtcctgcat caagtcataa gaa
//
LOCUS       DACCDF010000257        11422 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.087, whole genome shotgun sequence.
ACCESSION   DACCDF010000257 DACCDF010000000
VERSION     DACCDF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.087"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            39..977
                     /locus_tag="IVK51_002996"
                     /pseudo
     CDS             39..977
                     /locus_tag="IVK51_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="IVK51_002997"
     CDS             980..3163
                     /locus_tag="IVK51_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP6432492.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="IVK51_002998"
     CDS             3160..4161
                     /locus_tag="IVK51_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP6432493.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="IVK51_002999"
     CDS             4176..5090
                     /locus_tag="IVK51_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP6432494.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="IVK51_003000"
     CDS             5335..5451
                     /locus_tag="IVK51_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP6432495.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVK51_003001"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="IVK51_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP6432496.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVK51_003002"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="IVK51_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP6432497.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="IVK51_003003"
     CDS             9421..10683
                     /locus_tag="IVK51_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP6432498.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11410
                     /locus_tag="IVK51_003004"
     CDS             10928..11410
                     /locus_tag="IVK51_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP6432499.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNGSVAKF
                     "
BASE COUNT         3771 a         1907 c         2388 g         3356 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaagga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatg gttctgttgc
    11401 aaagttttaa ataaagaata aa
//
LOCUS       DACCDF010000258         7959 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.106, whole genome shotgun sequence.
ACCESSION   DACCDF010000258 DACCDF010000000
VERSION     DACCDF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.106"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            128..1564
                     /locus_tag="IVK51_003005"
     CDS             128..1564
                     /locus_tag="IVK51_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP6432500.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IVK51_003006"
     CDS             1753..4200
                     /locus_tag="IVK51_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP6432501.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK51_003007"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IVK51_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP6432502.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IVK51_003008"
     CDS             complement(5690..6793)
                     /locus_tag="IVK51_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP6432503.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK51_003009"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IVK51_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP6432504.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACCDF010000259         7482 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.109, whole genome shotgun sequence.
ACCESSION   DACCDF010000259 DACCDF010000000
VERSION     DACCDF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.109"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            109..192
                     /locus_tag="IVK51_003010"
     CDS             109..192
                     /locus_tag="IVK51_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HAP6432505.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK51_003011"
     CDS             317..1054
                     /gene="erm(B)"
                     /locus_tag="IVK51_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP6432506.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1224..1484
                     /locus_tag="IVK51_003012"
                     /pseudo
     CDS             1224..1484
                     /locus_tag="IVK51_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            1647..2312
                     /locus_tag="IVK51_003013"
                     /pseudo
     CDS             1647..2312
                     /locus_tag="IVK51_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            2348..2593
                     /locus_tag="IVK51_003014"
     CDS             2348..2593
                     /locus_tag="IVK51_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP6432507.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            2696..3565
                     /locus_tag="IVK51_003015"
     CDS             2696..3565
                     /locus_tag="IVK51_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP6432508.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3546..4280
                     /locus_tag="IVK51_003016"
     CDS             3546..4280
                     /locus_tag="IVK51_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP6432509.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4313..5225
                     /gene="ant(6)"
                     /locus_tag="IVK51_003017"
                     /pseudo
     CDS             4313..5225
                     /gene="ant(6)"
                     /locus_tag="IVK51_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            5222..5764
                     /gene="sat4"
                     /locus_tag="IVK51_003018"
     CDS             5222..5764
                     /gene="sat4"
                     /locus_tag="IVK51_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAP6432510.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            5857..6651
                     /locus_tag="IVK51_003019"
                     /note="aph(3')-IIIa"
     CDS             5857..6651
                     /locus_tag="IVK51_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP6432511.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(6759..6947)
                     /locus_tag="IVK51_003020"
                     /pseudo
     CDS             complement(6759..6947)
                     /locus_tag="IVK51_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6927..7469
                     /locus_tag="IVK51_003021"
     CDS             6927..7469
                     /locus_tag="IVK51_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP6432512.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPVLLQSFK"
BASE COUNT         2623 a         1131 c         1567 g         2161 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccatt ttgtgtataa taggaattga
       61 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
      121 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
      181 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
      241 cagataacta aaattacaaa caaatcgttt aacttctgta tttatttaca gatgtaataa
      301 cttcaggagt gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga
      361 aaaagtactc aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga
      421 aattggaaca ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac
      481 gtctattgaa ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaacat
      541 tcgtgtcact ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta
      601 taaaattgtt gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt
      661 tgaaagccat gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt
      721 ggatattcac cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct
      781 taagctgcca gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact
      841 tacccgccat accacagatg ttccagataa atattggaag ctatatacgt actttgtttc
      901 aaaatgggtc aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat
      961 gaaacacgcc aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt
     1021 taatagttat ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg
     1081 gaaagttaca cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt
     1141 ccaagaagct aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa
     1201 ttcccactaa gcgctcggga ccccttgtag gaaaatgtcc taagtgtggc aacaatattg
     1261 tattaaaaaa atcgttttat ggttgttcaa attatcctga atgtaagttt actttagctg
     1321 aacattttag aaagaaaaaa ctaaccaaaa cgaatgtaaa agaattactg gagggaaaag
     1381 aaaccctggt aaaaggaatc aaaaacaaag agaaaaagcc ctacaatgcc gttgtaaaaa
     1441 ttggggaaaa gggatatatt gattttatct ctttttcaaa ataaacatgg ggctgaccca
     1501 aaagtagctc ctatatgaaa aatagaggtc tttccattta gatagtagtg gaaagacctc
     1561 tatttatctt ttgaaagtga atacaaaaat aaagcacact ctgctataat ataggtgatt
     1621 aatccatttg agagaggtgc tttatcatgt atcaaaatta taccataaac caactttgtt
     1681 tgcctataga tcttgaaatt aaattagaag aaaatgattt tgctcatgcc attgtgcagt
     1741 ttgttgactc tattcccgat gaagtctttc ttccttatta tcaatctatg ggtagacccc
     1801 aatatcatcc tcgtatgcta ctttccatta ttctttgtgc ctatattcaa ggggtttatt
     1861 cgggccgcca aatacaaaat atgctgatag atagtattcg gatgcgctac ttatctcaag
     1921 agcaattccc taactttcgt actattaatc gttttcgcgt tcatccgatt atgaacgata
     1981 ttctagatca ttcatttgtt caatttcgtg agctactggt tcaaagtggc ttaatcagtg
     2041 gcagtgctct atacattgat ggtacaaaaa ttgaagcgga tgcgaataaa tactctttcg
     2101 tttggaaaaa aagtatttta aagtataagg atagccttga tcaaaaagcc cttgaaaact
     2161 accaacaaat ggtggaaaca aaaatcttac ctgagttaat agatgattta aaggatgaac
     2221 tttctataga agagattgaa aggattcgtc aatctttgga agaaaaggaa aatcaattaa
     2281 ttgaagcgat tgaccaaacc gaaacagtag aagggggctg acccattatt aataaggagt
     2341 ggacaacatg attgatgtaa atgagctatt ggaagaagct attagagaaa ctgaaaatct
     2401 aaatgatggc gaagtttttc ttgttaaaga tttattcaag ggatatgtgg ggaataggat
     2461 acccagaaag gatcgacttc tgcttggaac gttattttta aaccatgtta gcaaaatgaa
     2521 tggtaattta aaggctattg aaaagacttc atctaatcag cagaggtata aaaagacaat
     2581 tgataagttt taactatctt ttgtgaaatt aaaaaaatta taaatatgtt gagtagaagc
     2641 aaaagatttg gcttggaatt tttacagcag tgaatcgata aaaggagcat gcttgatggt
     2701 agataatatt attaaatcag tagcagagaa attatcctct ctgtcttata tagaaggtat
     2761 tgttttaggt ggttcacgtg caaggggcac ccatacagag gattcggata tagatatcgg
     2821 catctattac aattcagaat catttgacat aaatactatt aatcaattcg ctacaaagct
     2881 ggatgatgag catagaaata accttgttgt acctcccgga gcatggggtg attggattaa
     2941 tggcggcgga tggttagtca taaacgggta tcatgtggat ttaattttac gtgatattaa
     3001 acgtgtggaa caaataatga aagatacaga gcacggaatt gttactgcca attatcagac
     3061 tgggcatccc catggttata ttagtgcaat gtatcgagga gaattagcga ttagcaaaat
     3121 actatatgct aagaatgaaa gcttatgcga attaaaaaaa caggcagaaa cttatcccaa
     3181 tgctttgcag aaaagtttag ttaacttttt tatgtttgaa gcagggttct ctttaatgtt
     3241 tgtaaaagca aattcgggaa cagacgataa atattatatt gcgggtcatg tttttcgtat
     3301 agtttcatgt ttaaatcaag tgttatttgc atgtaataat gcttattgta tcaacgaaaa
     3361 gaaagctata aaactgcttg aaacttttga acataaacct gaaaaatata ccgagaaggt
     3421 aaatcatatt tttgaagtac tcggtatctc actttttgaa tgctacgaca tgaccgagaa
     3481 gctttataat gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag
     3541 ttcagatgaa agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga
     3601 gtcgctcgca gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac
     3661 ctgattttaa gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat
     3721 atgcgatgga aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg
     3781 aagtagcaaa aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag
     3841 atgtggattt cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg
     3901 tagcagacta tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt
     3961 tagtttttac agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata
     4021 acgaaaaagg agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga
     4081 cagctatgtt tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa
     4141 atacactgct ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga
     4201 acatgatgga tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat
     4261 cggcaaaaaa gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc
     4321 agaaaaagaa atgatggatt tagtactttc tttagcagaa caggatgaac gaacgtattc
     4381 gaattgtgac ccttgagggg tcacgcgcaa atattaatat acctaaagat gaatttcagg
     4441 attatgatat tacatatttt gtaagtgata tagaaccgtt tatatctaat gatgactggc
     4501 ttaatcaatt tgggaatata ataatgatgc aaaagccgga ggatatggaa ttattcccac
     4561 ctgaagaaaa gggattttcc tatcttatgc tatttgatga ttacaataaa attgatctta
     4621 ccttattgcc cttggaagag ttagataatt acctaaaggg cgataaatta ataaaggttc
     4681 taattgataa agattgtaga attaaaaggg acatagttcc gactgatata gattatcatg
     4741 taagaaagcc aagcgcaagg gagtatgatg attgctgcaa tgaattttgg aatgtaacac
     4801 cttatgttat taaaggattg tgccgtaagg aaattttatt tgctattgat cattttaatc
     4861 agattgttcg ccatgagctg ctgagaatga tatcatggaa ggtcggcatc gaaacaggct
     4921 ttaaattaag tgtaggcaag aactataagt ttattgaaag gtatatatcc gaggatttgt
     4981 gggagaaact tttgtccacc taccggatgg attcctatga aaacatatgg gaagcattat
     5041 ttctatgcca tcaattgttc agggcggtat ccggtgaggt ggcggaaagg cttcattatg
     5101 cctatccgga gtatgatagg aatataacaa aatataccag ggacatgtat aaaaaataca
     5161 ctggtaaaac cggctgcctg gatagcacat atgccgctga tatagaagag aggcgggaac
     5221 agtgattaca gaaatgaaag cagggcacct gaaagatatc gataaaccca gcgaaccatt
     5281 tgaggtgata ggtaagatta taccgaggta tgaaaacgag aattggacct ttacagaatt
     5341 actctatgaa gcgccatatt taaaaagcta ccaagacgaa gaggatgaag aggatgagga
     5401 ggcagattgc cttgaatata ttgacaatac tgataagata atatatcttt actaccaaga
     5461 cgataaatgc gtcggaaaag ttaaactgcg aaaaaattgg aaccggtacg cttatataga
     5521 agatatcgcc gtatgtaagg atttcagggg gcaaggcata ggcagcgcgc ttatcaatat
     5581 atctatagaa tgggcaaagc ataaaaactt gcatggacta atgcttgaaa cccaggacaa
     5641 taaccttata gcttgtaaat tctatcataa ttgtggtttc aaaatcggct ccgtcgatac
     5701 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     5761 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     5821 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     5881 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     5941 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     6001 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     6061 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     6121 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     6181 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     6241 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     6301 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     6361 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     6421 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     6481 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     6541 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     6601 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagat
     6661 ttagatgtct aaaaagcttt aactacaagc tttttagaca tctaatcttt tctgaagtac
     6721 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagagt
     6781 tctatattag agatgtaaag agttttggtg aagaggtttg tgagtatggc ttttataacc
     6841 agggtgcaat gagaagcact aacagcagct agcttaagaa cgcataggat atacttttta
     6901 tggaagtcac agaggaggaa taaaatttga acaggattaa cagagtaacc tctattctta
     6961 ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat tgcacagcga tttaatataa
     7021 gcttaaggac agtttacaga gatatccgga cacttgaaga agccggaata ccaattggat
     7081 ctgaggccgg aaaaggatat tttcttgtag agggcttcct acttccgccg gtaatgttta
     7141 cagcggcgga agtgggtgca ttgattacag cagggaaatt tttaaattgc catggagatg
     7201 aatcatttat aaaggatttt gattcagcta tgtataaaat caaatctatt ttgaagcatg
     7261 gtgaaaaaaa ctatgcacag gagttggaga acagtattaa cgtgtacagc acatctggac
     7321 agaaaaatac attggcggat aacgttattg cagcaattca gactgcaatc tgcaataaaa
     7381 gggtaatatc aattcaatat cctgcatcag gagggcaaga accggaaagc agaatgatag
     7441 agccggttct gttgcaaagt tttaaataaa gaataaaatc cc
//
LOCUS       DACCDF010000260         4995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.125, whole genome shotgun sequence.
ACCESSION   DACCDF010000260 DACCDF010000000
VERSION     DACCDF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.125"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            complement(30..2180)
                     /locus_tag="IVK51_003022"
                     /pseudo
     CDS             complement(30..2180)
                     /locus_tag="IVK51_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2326..2901
                     /locus_tag="IVK51_003023"
     CDS             2326..2901
                     /locus_tag="IVK51_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432513.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3115..3810
                     /gene="vanR"
                     /locus_tag="IVK51_003024"
     CDS             3115..3810
                     /gene="vanR"
                     /locus_tag="IVK51_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP6432514.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            3788..4942
                     /locus_tag="IVK51_003025"
     CDS             3788..4942
                     /locus_tag="IVK51_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP6432515.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1560 a          939 c          967 g         1529 t
ORIGIN      
        1 aattttcttc tttcagcttt ttatttccca cttgtaaatc ggtagatggt ggctcctttt
       61 ccagttccat aatgtggatt tgcatctgca tgtagtgatg aaacacctag ctgcattctc
      121 ataccatctg acgaagatgt tgtaccgtcg ccccaataga aaggcaattg taatttatga
      181 tgaaagttta ctaatatggc ttgggcttta ttcatggcat cttcatacat gcgccattga
      241 gatacattgg ctagttgctt atatgtaagt ccgggtgtgg cttcggccat cttgctcaag
      301 ccaatattca ttcccattcc taaaagggca gccatgataa tgattgtttc ttccttatct
      361 ggttttcgat tattggaagc atgagtgaat tgctcatgaa atcctgttat atgggccaca
      421 tccatgagta aatcagttaa ttttattctt ggtagcatct gataaaggct tgcactaaat
      481 ttttttgctt cttctggaac atctttttct aagcgtgcaa gtgatagctt tcctttttca
      541 agagaaaccc catctaactt attggaattg gcagctaacc actttaacct ttcattaaag
      601 ctgctggttc tctccgttat ataatcttcg aatgataaac taactgataa tctcgtattc
      661 cccttcgatt gattccatgt atcttccgaa aacaaatatt cctcaaaatc cctatattgt
      721 ctgctgccaa caatggaaac atctcctgcc cgaacatgct cccgaagttc tgttaaaaca
      781 gccatttcat agtaatgacg attaattgtt gtaccatcat cctcgtataa atgtcttttc
      841 catcgttttg aaataaaatc cacaggtgag tcatcaggca cttttcgctt tccagattcg
      901 ttcattcctc ggataatctc aacagcttgt aaaagtggct catttgcctt tgtagaatga
      961 aattccaata ctcttaatag cgttggcgta tattttctta gtgaataaaa ccgtttttgc
     1021 agtaagtcta aataatcata gtcggcagga cgtgcaagtt cctgagcctc ttctactgaa
     1081 gagacaaagg tattccattc aataaccgat tctaaaacct taaaaacgtc taatttttcc
     1141 tctcttgctt taattaatgc ttgtccgatg ttcgtaaagt gtataacttt ctcatttagc
     1201 tttttaccgt tttgtttctg gatttcctct tgagccttac gaccttttga taacaaacta
     1261 agtatttgcc tatcatgaat ttcaaacgct ttatccgtta gctcctgagt aagttgtaat
     1321 aaatagatgg ttaatatcga ataacgttta ttttcttgaa agtcacggaa tgcatacggc
     1381 tcgtatcttg agcctaagcg agacagctgc aacaggcggt tacggtgcaa atgactaatt
     1441 tgcactgttt ctaaatccat tcctcgtatg tattcgagtc gttctattat ttttagaaaa
     1501 gtttcgggtg aaggatgacc cggtggctct tttaaccaac ccaatatcgt tttattggat
     1561 tcggatggat gctgcgaggt aataatccct tcaagctttt ctttttgctc atttgttaga
     1621 gatttactaa ccgtattaaa tagcttcttt tcagccattg cccttgcttc ccacaccatt
     1681 ctttcaagtg tagtgatagc aggcagtata attttgtttt ttcttagaaa atctatgcat
     1741 tcatgcagta gatgaatggc atcaccattt tccaaagcta attgatgaag gtacttaaat
     1801 gtcattcgat attcactcag ggtaaaagtt acaaagtcgt attcacttcg aatttctttc
     1861 aaatgatccc aaagtgtatt ttccctttga ggataatgat caagcgagga tggactaaca
     1921 ccaatctgtt tcgatatata ttgtatgacc gaatctggga tgcttttgat atgagtgtat
     1981 ggccaaccgg gataccgaag aacagctaat tgaacagcaa atcctaaacg gttttcttcc
     2041 ctccttcgct tattaactat ttctaaatcc cgtttggaaa aagtgaagta ggtccccagt
     2101 atccattcat cttcagggat ttgcataaaa gcctgtctct gttccggtgt aagcaattct
     2161 ctacctctcg caattttcat tcagtatcat tccatttctg tattttcaat ttattagttc
     2221 aattatatat caatagagtg tactctattg atacaaatgt agtagactga taaaatcata
     2281 gttaagagcg tctcataaga cttgtctcaa aaatgaggtg atattttgcg gaaaatcggt
     2341 tatattcgtg tcagttcgac taaccagaat ccttcaagac aatttcagca gttgaacgag
     2401 atcggaatgg atattatata tgaagagaaa gtttcaggag caacaaagga tcgcgagcaa
     2461 cttcaaaaag tgttagacga tttacaggaa gatgacatca tttatgttac agacttaact
     2521 cgaatcactc gtagtacaca agatctattt gaattaatcg ataacatacg agataaaaag
     2581 gcaagtttaa aatcactaaa agatacatgg cttgatttat cagaagataa tccatacagc
     2641 caattcttaa ttactgtaat ggctggtgtt aaccaattag agcgagatct tattcggatg
     2701 agacaacgtg aagggattga attggctaag aaagaaggaa agtttaaagg tcgattaaag
     2761 aagtatcata aaaatcacgc aggaatgaat tatgcggtaa agctatataa agaaggaaat
     2821 atgactgtaa atcaaatttg tgaaattact aatgtatcta gggcttcatt atacaggaaa
     2881 ttatcagaag tgaataatta gccattctgt attccgctaa tgggcaatat ttttaaagaa
     2941 gaaaaggaaa ctataaaata ttaacagcct cctagcgatg ccgaaaagcc ctttgataaa
     3001 aaaagaatca tcatcttaag aaattcttag tcatttatta tgtaaatgct tataaattcg
     3061 gccctataat ctgataaatt attaagggca aacttatgtg aaagggtgat aactatgagc
     3121 gataaaatac ttattgtgga tgatgaacat gaaattgccg atttggttga attatactta
     3181 aaaaacgaga attatacggt tttcaaatac tataccgcca aagaagcatt ggaatgtata
     3241 gacaagtctg agattgacct tgccatattg gacatcatgc ttcccggcac aagcggcctt
     3301 actatctgtc aaaaaataag ggacaagcac acctatccga ttatcatgct gaccgggaaa
     3361 gatacagagg tagataaaat tacagggtta acaatcggcg cggatgatta tataacgaag
     3421 ccctttcgcc cactggagtt aattgctcgg gtaaaggccc agttgcgccg atacaaaaaa
     3481 ttcagtggag taaaggagca gaacgaaaat gttatcgtcc actccggcct tgtcattaat
     3541 gttaacaccc atgagtgtta tctgaacgag aagcagttat cccttactcc caccgagttt
     3601 tcaatactgc gaatcctctg tgaaaacaag gggaatgtgg ttagctccga gctgctattt
     3661 catgagatat ggggcgacga atatttcagc aagagcaaca acaccatcac cgtgcatatc
     3721 cggcatttgc gcgaaaaaat gaacgacacc attgataatc cgaaatatat aaaaacggta
     3781 tggggggttg gttataaaat tgaaaaataa aaaaaacgac tattccaaac tagaacgaaa
     3841 actttacatg tatatcgttg caattgttgt ggtagcaatt gtattcgtgt tgtatattcg
     3901 ttcaatgatc cgagggaaac ttggggattg gatcttaagt attttggaaa acaaatatga
     3961 cttaaatcac ctggacgcga tgaaattata tcaatattcc atacggaaca atatagatat
     4021 ctttatttat gtggcgattg tcattagtat tcttattcta tgtcgcgtca tgctttcaaa
     4081 attcgcaaaa tactttgacg agataaatac cggcattgat gtacttattc agaacgaaga
     4141 taaacaaatt gagctttctg cggaaatgga tgttatggaa caaaagctca acacattaaa
     4201 acggactctg gaaaagcgag agcaggatgc aaagctggcc gaacaaagaa aaaatgacgt
     4261 tgttatgtac ttggcgcacg atattaaaac gccccttaca tccattatcg gttatttgag
     4321 cctgcttgac gaggctccag acatgccggt agatcaaaag gcaaagtatg tgcatatcac
     4381 gttggacaaa gcgtatcgac tcgaacagct aatcgacgag ttttttgaga ttacacggta
     4441 taacctacaa acgataacgc taacaaaaac gcacatagac ctatactata tgctggtgca
     4501 gatgaccgat gaattttatc ctcagctttc cgcacatgga aaacaggcgg ttattcacgc
     4561 ccccgaggat ctgaccgtgt ccggcgaccc tgataaactc gcgagagtct ttaacaacat
     4621 tttgaaaaac gccgctgcat acagtgagga taacagcatc attgacatta ccgcgggcct
     4681 ctccggggat gtggtgtcaa tcgaattcaa gaacactgga agcatcccaa aagataagct
     4741 agctgccata tttgaaaagt tctataggct ggacaatgct cgttcttccg atacgggtgg
     4801 cgcgggactt ggattggcga ttgcaaaaga aattattgtt cagcatggag ggcagattta
     4861 cgcggaaagc aatgataact atacgacgtt tagggtagag cttccagcga tgccagactt
     4921 ggttgataaa aggaggtcct aagagatgta tataattttg gctctttgtc aaatgatgtt
     4981 ggtaaagaga aatct
//
LOCUS       DACCDF010000261         4853 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.126, whole genome shotgun sequence.
ACCESSION   DACCDF010000261 DACCDF010000000
VERSION     DACCDF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.126"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            352..1263
                     /locus_tag="IVK51_003026"
     CDS             352..1263
                     /locus_tag="IVK51_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAP6432516.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1416..1901
                     /gene="vanZ"
                     /locus_tag="IVK51_003027"
     CDS             1416..1901
                     /gene="vanZ"
                     /locus_tag="IVK51_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAP6432517.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2188..2517)
                     /locus_tag="IVK51_003028"
     CDS             complement(2188..2517)
                     /locus_tag="IVK51_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP6432518.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2822..3151
                     /locus_tag="IVK51_003029"
     CDS             2822..3151
                     /locus_tag="IVK51_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP6432519.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3141..3428
                     /locus_tag="IVK51_003030"
     CDS             3141..3428
                     /locus_tag="IVK51_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP6432520.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3707..4270)
                     /locus_tag="IVK51_003031"
     CDS             complement(3707..4270)
                     /locus_tag="IVK51_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP6432521.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1699 a          735 c          834 g         1585 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccgg cagagcgata ttgcggtcat
       61 tatctgcgtg cgctgcggca agatagcctg ataataagac tgatcgcata gaggggtggt
      121 atttcacacc gcccattgtc aacaggcagt tcagcctcgt taaattcagc atgggtatca
      181 cttatgaaaa ttcatctaca ttggtgataa tagtaaatcc agtagggcga aataattgac
      241 tgtaatttac ggggcaaaac ggcacaatct caaacgagat tgtgccgttt aaggggaaga
      301 ttctagaaat atttcatact tccaactata tagttaagga ggagactgaa aatgaagaag
      361 ttgttttttt tattgttatt gttattctta atatacttag gttatgacta cgttaatgaa
      421 gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc caaagaacat
      481 ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga agaacaggtt
      541 tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga aagtgtgaag
      601 tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg gttgcttgat
      661 agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt caatgatgct
      721 gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt tgatgagcaa
      781 agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta tagtgagcat
      841 aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg agcccctgaa
      901 ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta tccagaggac
      961 aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt tggtttacca
     1021 catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga ttacctaaaa
     1081 gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt ttattatcct
     1141 gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat atcaggaaac
     1201 aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa ttcaaggagg
     1261 taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac tagcactttt
     1321 ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc tatactaatg
     1381 atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta tctagaggat
     1441 tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta caatacaata
     1501 ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt actgctactg
     1561 ggaatttcag agagatgata gataatgtta taatctttat tccatttggc ttgcttttga
     1621 atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg gttttaagtc
     1681 ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata acagatgtaa
     1741 ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc aataagcata
     1801 tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg ctcgtattat
     1861 tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa atcaagcaat
     1921 ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa acgggatgtg
     1981 gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta aaaagcttac
     2041 ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg aattggtctc
     2101 tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc ccaaactaag
     2161 atttcataga tagattttct gataacttta aaaaacacga atagcggaaa ggatgtcttc
     2221 attcgctaaa gagtaataaa ctacttttcc atcttgacgt gattttgcca ttccatgttt
     2281 ttttaaaaaa cgtaaatggt gtgacgttgt agcgacacta gcagtaagta tttctgctaa
     2341 atcacagacg cacatttcct tataggtttc taaagcatag aaaattttaa ttctagaaaa
     2401 atcgctgaaa catttcccta acacaagtaa gtttgaaaaa tctttttgat ccaatttatt
     2461 ttttattcca tttacttttt ctttatgtac gacagtcact tggcaaactt ttttcattca
     2521 tctcacctca cattcaaacg attatttgaa tgaagtatac ctatttgatc actcttttgt
     2581 caatctacct taattccaat acactattat aagcaatttc atgtattgaa gtaaacttac
     2641 ttgcaagtgc gtttttgatt ccaagttaaa aacatattat tattctacat aatttcctaa
     2701 tcaaataaaa ctcctttgaa aattgttcta aaggagtttt attattttgt atttctacta
     2761 aaaatagcgt acaatgtaat tactaagtaa ttacgaaatg taaaataatg gaggaaataa
     2821 tatgattaca aaaacaagaa aacaaggaaa ttctattatg ttaacggtcc ctaaagaatt
     2881 cgatgtgccc aatggtgttg aagtagaagc taagcttgta gagaatggta tcctttatga
     2941 gtttgtagag cctaaaaaag aattttttga ttttagtgaa gatattttgg ctgatattct
     3001 ttcagaaggt tatagcaaac aagagatctt aaaagaattt agaaatagaa aaaatgcgtt
     3061 aacttctgca tttaaatcta ttgctgaaga tactgttgca aattctaaac cgatgacaaa
     3121 agaggagttg gcagcagaaa ttggcttatg agattctacc ttctaaacac gtaataaagt
     3181 atctaaaaaa attaaaagag aaaacattaa aagaacaatt tttaacgata atttatgatg
     3241 agatagcagt tcgtcctcat tcgggagaac aaaaaacggg tgatttatca ggaatttggg
     3301 ctattggatt caagtatgca ggtacgacat atcgcgttgc atatgaaatt aaagataatg
     3361 cagtgatacc tatcttacta tgtggaacgc atgaaaattt ttatgaacaa ttaaaaaaga
     3421 ttaggtaaga acccaatgtt tcaacttgag aggatagacg acactttaaa agataatttt
     3481 taacagctga gagttgtaat accctataat tttaaaccac taacctatat atttatgaaa
     3541 aatagaaaaa attaaaacgc attttaagcc tctttcaaat taaaagcttc atccttatgc
     3601 acaaagaggg cttaaaatgc gttattttat attgtttttt agtaataata aaaaaatcac
     3661 tctaattctc ccataaatat tatagattcc taaagattaa aatagattat tccctattgt
     3721 cttttaagta gcggtagagt gttgccttgc taataccagt catttccgta atttctttta
     3781 cgctataaac ctgactttta tacaatttta ctgctcgatc aacatctttc tggttcactc
     3841 gtggacggcc acctttacga cctctcgcac gtgcactttt caaaccttct ttggtacgtt
     3901 caacaattaa atcacgttcg aactgactaa atgcttggaa gacagtcatc attaagcgtc
     3961 cgtgaggcgt gctggtatcg aagttttctt tcaagctcac cagcttaaca tggtgttctt
     4021 caaaataggt aacaaggtca atcaaatcct ttgtactacg acccaatcga gaaaaacttt
     4081 ctacaattac cgtatctccc ggacgaattt tatcttttaa acgatttagt tggggacgaa
     4141 ccgctttggt tcccgtcatt ttctcagtta ggatttcatt acaattttgt tcattgagta
     4201 agtctaattg acgatttagt tcttgtgaac gtgtactcac tcgggcataa ccataaatgt
     4261 atccactcat gcttttcttc ctttcgtact ttaattctgg cttgattgta tcataaaagg
     4321 gagttataga tacatagtta atgcaacaag ttatgataca aaatatgacc tcttttattt
     4381 aataaaagag gtcatttcat tttgtctcat aatcgattgt ttttgatacg agtgtgtata
     4441 cgaaataaac aaaaaaatct taagtaaatt tctagatacc taatttaatc ctaactagtg
     4501 tatcctaaag ttaataacga gtttatcaca atgaaaatta taaaatgctc ccatattaga
     4561 tataacatgc aatatttgaa ttagaactac aaaatttatt cgttttataa agtaaataat
     4621 gttgagatac taaggaatag aatattataa ggaattgagc tgaatgtatt ggataaaaaa
     4681 agaataactt tattagagat ggactataaa aatgctcgta attttcttct gaagtctgaa
     4741 agctatttga atgctcagct accagaatat attagttttg acgaaacttt aaaatctgct
     4801 aaaaaagttt taacttcgcc gtttgtataa ttaagcgaga caaataaaaa agc
//
LOCUS       DACCDF010000262         2999 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.166, whole genome shotgun sequence.
ACCESSION   DACCDF010000262 DACCDF010000000
VERSION     DACCDF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.166"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            245..1213
                     /locus_tag="IVK51_003032"
     CDS             245..1213
                     /locus_tag="IVK51_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP6432522.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="IVK51_003033"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="IVK51_003033"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP6432523.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="IVK51_003034"
     CDS             2243..2851
                     /locus_tag="IVK51_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP6432524.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          722 g          809 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DACCDF010000263         1650 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain Vancomycin resistant
            Enterococcus faecium isolate VRECG28-17-B
            SAMN13750740-rid10751803.guided.191, whole genome shotgun sequence.
ACCESSION   DACCDF010000263 DACCDF010000000
VERSION     DACCDF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN13750740
            Sequence Read Archive: SRR10270747
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-OCT-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000860329.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000860329.1
            Genome Coverage       :: 424x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/13/2020 19:08:42
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,036
            CDSs (total)                      :: 2,972
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 75
            CDSs (without protein)            :: 75
            Pseudo Genes (ambiguous residues) :: 0 of 75
            Pseudo Genes (frameshifted)       :: 35 of 75
            Pseudo Genes (incomplete)         :: 32 of 75
            Pseudo Genes (internal stop)      :: 21 of 75
            Pseudo Genes (multiple problems)  :: 12 of 75
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN13750740-rid10751803.guided.191"
                     /strain="Vancomycin resistant Enterococcus faecium"
                     /isolate="VRECG28-17-B"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Memphis, TN"
                     /lat_lon="35.15 N 90.04 W"
                     /collection_date="2008/2018"
                     /collected_by="St. Jude Children's Research Hospital"
     gene            <1..1348
                     /locus_tag="IVK51_003035"
     CDS             <1..1348
                     /locus_tag="IVK51_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP6432525.1"
                     /translation="DFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQI
                     YKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAV
                     ILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKY
                     LIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNT
                     NLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLR
                     QMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFE
                     GKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGE
                     DILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1478..>1650)
                     /locus_tag="IVK51_003036"
     CDS             complement(1478..>1650)
                     /locus_tag="IVK51_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP6432526.1"
                     /translation="KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEW
                     IYSSRKYINFDK"
BASE COUNT          671 a          148 c          278 g          553 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgaa tcattaaaaa aacattatac
       61 agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg
      121 atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac
      181 tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg
      241 aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa
      301 tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc
      361 tggttttaga attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga
      421 ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata
      481 tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg
      541 ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa aatttagtac
      601 taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa atacaaattt
      661 agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg aattatctat
      721 actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt ctactatgtc
      781 agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac aaatgcacgg
      841 tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg tattagaaga
      901 gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa aagattatat
      961 agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa agtgtttatg
     1021 ccataatgat tttagttgta atcatctatt gttagatggc aataatagat taactggaat
     1081 aattgatttt ggagattctg gaattataga tgaatattgt gattttatat acttacttga
     1141 agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa tgtatggaaa
     1201 tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt atcctattga
     1261 aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg gtagaaaaga
     1321 aatttataaa aggacttata aagattgatt atataatata tgaaaagcta ttataaaaga
     1381 cattagtatt aaatagttta aaaaaatgaa aaataataaa ggaagtgagt caagtccaga
     1441 ctcctgtgta aaatgctata caatgttttt accatttcta cttatcaaaa ttgatgtatt
     1501 ttcttgaaga ataaatccat tcatcatgta ggtccataag aacggctcca attaagcgat
     1561 tggctgatgt ttgattgggg aagatgcgaa taatcttttc tcttctgcgt acttcttgat
     1621 tcagtcgttc aattagattg gtactcttta
//
